LOCUS       DACCXO010000001        75361 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.001, whole genome shotgun sequence.
ACCESSION   DACCXO010000001 DACCXO010000000
VERSION     DACCXO010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 75361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.001"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            225..1508
                     /locus_tag="IVY70_000001"
     CDS             225..1508
                     /locus_tag="IVY70_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7957169.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            1524..2411
                     /locus_tag="IVY70_000002"
     CDS             1524..2411
                     /locus_tag="IVY70_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7957170.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            2428..3264
                     /locus_tag="IVY70_000003"
     CDS             2428..3264
                     /locus_tag="IVY70_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7957171.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            3267..3890
                     /locus_tag="IVY70_000004"
     CDS             3267..3890
                     /locus_tag="IVY70_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957172.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            3887..5179
                     /locus_tag="IVY70_000005"
     CDS             3887..5179
                     /locus_tag="IVY70_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7957173.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      5538..5635
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5751..6332
                     /locus_tag="IVY70_000006"
     CDS             5751..6332
                     /locus_tag="IVY70_000006"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP7957174.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            6335..7642
                     /locus_tag="IVY70_000007"
     CDS             6335..7642
                     /locus_tag="IVY70_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP7957175.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            8058..8546
                     /gene="purE"
                     /locus_tag="IVY70_000008"
     CDS             8058..8546
                     /gene="purE"
                     /locus_tag="IVY70_000008"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP7957176.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            8530..9663
                     /gene="purK"
                     /locus_tag="IVY70_000009"
     CDS             8530..9663
                     /gene="purK"
                     /locus_tag="IVY70_000009"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP7957177.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            9676..10971
                     /gene="purB"
                     /locus_tag="IVY70_000010"
     CDS             9676..10971
                     /gene="purB"
                     /locus_tag="IVY70_000010"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP7957178.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(11151..11528)
                     /locus_tag="IVY70_000011"
     CDS             complement(11151..11528)
                     /locus_tag="IVY70_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957179.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            11784..12512
                     /locus_tag="IVY70_000012"
     CDS             11784..12512
                     /locus_tag="IVY70_000012"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP7957180.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            12512..12766
                     /gene="purS"
                     /locus_tag="IVY70_000013"
     CDS             12512..12766
                     /gene="purS"
                     /locus_tag="IVY70_000013"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP7957181.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            12768..13439
                     /gene="purQ"
                     /locus_tag="IVY70_000014"
     CDS             12768..13439
                     /gene="purQ"
                     /locus_tag="IVY70_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP7957182.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            13440..15662
                     /gene="purL"
                     /locus_tag="IVY70_000015"
     CDS             13440..15662
                     /gene="purL"
                     /locus_tag="IVY70_000015"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP7957183.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            15647..17086
                     /locus_tag="IVY70_000016"
     CDS             15647..17086
                     /locus_tag="IVY70_000016"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP7957184.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            17118..18161
                     /locus_tag="IVY70_000017"
     CDS             17118..18161
                     /locus_tag="IVY70_000017"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP7957185.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            18158..18736
                     /gene="purN"
                     /locus_tag="IVY70_000018"
     CDS             18158..18736
                     /gene="purN"
                     /locus_tag="IVY70_000018"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP7957186.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            18733..20268
                     /gene="purH"
                     /locus_tag="IVY70_000019"
     CDS             18733..20268
                     /gene="purH"
                     /locus_tag="IVY70_000019"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP7957187.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            20284..21558
                     /gene="purD"
                     /locus_tag="IVY70_000020"
     CDS             20284..21558
                     /gene="purD"
                     /locus_tag="IVY70_000020"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP7957188.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            21680..22594
                     /locus_tag="IVY70_000021"
     CDS             21680..22594
                     /locus_tag="IVY70_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP7957189.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(22727..24292)
                     /locus_tag="IVY70_000022"
     CDS             complement(22727..24292)
                     /locus_tag="IVY70_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7957190.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(24285..25736)
                     /locus_tag="IVY70_000023"
     CDS             complement(24285..25736)
                     /locus_tag="IVY70_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7957191.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIRESKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(25750..26766)
                     /locus_tag="IVY70_000024"
     CDS             complement(25750..26766)
                     /locus_tag="IVY70_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7957192.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            26986..28074
                     /locus_tag="IVY70_000025"
     CDS             26986..28074
                     /locus_tag="IVY70_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7957193.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            28128..29654
                     /locus_tag="IVY70_000026"
     CDS             28128..29654
                     /locus_tag="IVY70_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP7957194.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            29656..30861
                     /locus_tag="IVY70_000027"
     CDS             29656..30861
                     /locus_tag="IVY70_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7957195.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            31118..32095
                     /gene="guaC"
                     /locus_tag="IVY70_000028"
     CDS             31118..32095
                     /gene="guaC"
                     /locus_tag="IVY70_000028"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP7957196.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            32359..33783
                     /locus_tag="IVY70_000029"
     CDS             32359..33783
                     /locus_tag="IVY70_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP7957197.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            34244..35353
                     /gene="pdhA"
                     /locus_tag="IVY70_000030"
     CDS             34244..35353
                     /gene="pdhA"
                     /locus_tag="IVY70_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP7957198.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            35356..36333
                     /locus_tag="IVY70_000031"
     CDS             35356..36333
                     /locus_tag="IVY70_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP7957199.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            36358..38001
                     /locus_tag="IVY70_000032"
     CDS             36358..38001
                     /locus_tag="IVY70_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP7957200.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            38009..39415
                     /gene="lpdA"
                     /locus_tag="IVY70_000033"
     CDS             38009..39415
                     /gene="lpdA"
                     /locus_tag="IVY70_000033"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP7957201.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            39577..39852
                     /locus_tag="IVY70_000034"
     CDS             39577..39852
                     /locus_tag="IVY70_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP7957202.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            39858..40625
                     /locus_tag="IVY70_000035"
     CDS             39858..40625
                     /locus_tag="IVY70_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP7957203.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            40793..42622
                     /gene="typA"
                     /locus_tag="IVY70_000036"
     CDS             40793..42622
                     /gene="typA"
                     /locus_tag="IVY70_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP7957204.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            42883..44655
                     /gene="recQ"
                     /locus_tag="IVY70_000037"
     CDS             42883..44655
                     /gene="recQ"
                     /locus_tag="IVY70_000037"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP7957205.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            44769..45071
                     /locus_tag="IVY70_000038"
     CDS             44769..45071
                     /locus_tag="IVY70_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP7957206.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            45074..46237
                     /locus_tag="IVY70_000039"
     CDS             45074..46237
                     /locus_tag="IVY70_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP7957207.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            46263..49691
                     /locus_tag="IVY70_000040"
     CDS             46263..49691
                     /locus_tag="IVY70_000040"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP7957208.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            49712..50836
                     /locus_tag="IVY70_000041"
     CDS             49712..50836
                     /locus_tag="IVY70_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957209.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            50826..51263
                     /locus_tag="IVY70_000042"
     CDS             50826..51263
                     /locus_tag="IVY70_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP7957210.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            51247..51585
                     /locus_tag="IVY70_000043"
     CDS             51247..51585
                     /locus_tag="IVY70_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP7957211.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            51704..52264
                     /gene="rsmD"
                     /locus_tag="IVY70_000044"
     CDS             51704..52264
                     /gene="rsmD"
                     /locus_tag="IVY70_000044"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP7957212.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            52261..52752
                     /gene="coaD"
                     /locus_tag="IVY70_000045"
     CDS             52261..52752
                     /gene="coaD"
                     /locus_tag="IVY70_000045"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP7957213.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            52742..53785
                     /locus_tag="IVY70_000046"
     CDS             52742..53785
                     /locus_tag="IVY70_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP7957214.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            53944..54672
                     /locus_tag="IVY70_000047"
     CDS             53944..54672
                     /locus_tag="IVY70_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP7957215.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            54788..55033
                     /locus_tag="IVY70_000048"
     CDS             54788..55033
                     /locus_tag="IVY70_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7957216.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            55030..55248
                     /locus_tag="IVY70_000049"
     CDS             55030..55248
                     /locus_tag="IVY70_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP7957217.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            55384..55923
                     /locus_tag="IVY70_000050"
     CDS             55384..55923
                     /locus_tag="IVY70_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP7957218.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            55916..58189
                     /locus_tag="IVY70_000051"
     CDS             55916..58189
                     /locus_tag="IVY70_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP7957219.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            58227..59273
                     /gene="holA"
                     /locus_tag="IVY70_000052"
     CDS             58227..59273
                     /gene="holA"
                     /locus_tag="IVY70_000052"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP7957220.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(59362..59616)
                     /locus_tag="IVY70_000053"
     CDS             complement(59362..59616)
                     /locus_tag="IVY70_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP7957221.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(60016..60285)
                     /locus_tag="IVY70_000054"
     CDS             complement(60016..60285)
                     /locus_tag="IVY70_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957222.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(60755..61210)
                     /locus_tag="IVY70_000055"
     CDS             complement(60755..61210)
                     /locus_tag="IVY70_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP7957223.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(61348..63276)
                     /locus_tag="IVY70_000056"
     CDS             complement(61348..63276)
                     /locus_tag="IVY70_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP7957224.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(63473..64306)
                     /locus_tag="IVY70_000057"
     CDS             complement(63473..64306)
                     /locus_tag="IVY70_000057"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP7957225.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(64498..65202)
                     /locus_tag="IVY70_000058"
     CDS             complement(64498..65202)
                     /locus_tag="IVY70_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7957226.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(65225..65851)
                     /locus_tag="IVY70_000059"
     CDS             complement(65225..65851)
                     /locus_tag="IVY70_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7957227.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            66177..68552
                     /locus_tag="IVY70_000060"
     CDS             66177..68552
                     /locus_tag="IVY70_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP7957228.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(68576..69067)
                     /locus_tag="IVY70_000061"
     CDS             complement(68576..69067)
                     /locus_tag="IVY70_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP7957229.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            69254..70174
                     /locus_tag="IVY70_000062"
     CDS             69254..70174
                     /locus_tag="IVY70_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7957230.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            70328..70672
                     /locus_tag="IVY70_000063"
     CDS             70328..70672
                     /locus_tag="IVY70_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP7957231.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(70862..71362)
                     /locus_tag="IVY70_000064"
     CDS             complement(70862..71362)
                     /locus_tag="IVY70_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7957232.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            71890..71973
                     /locus_tag="IVY70_000065"
     tRNA            71890..71973
                     /locus_tag="IVY70_000065"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:71924..71926,aa:Leu,seq:cag)
     gene            complement(72297..72935)
                     /locus_tag="IVY70_000066"
     CDS             complement(72297..72935)
                     /locus_tag="IVY70_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7957233.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            73270..73704
                     /locus_tag="IVY70_000067"
     CDS             73270..73704
                     /locus_tag="IVY70_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7957234.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            73727..74047
                     /locus_tag="IVY70_000068"
     CDS             73727..74047
                     /locus_tag="IVY70_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957235.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            74188..74379
                     /locus_tag="IVY70_000069"
     CDS             74188..74379
                     /locus_tag="IVY70_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957236.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(74450..74707)
                     /locus_tag="IVY70_000070"
     CDS             complement(74450..74707)
                     /locus_tag="IVY70_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP7957237.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            74865..75128
                     /locus_tag="IVY70_000071"
     CDS             74865..75128
                     /locus_tag="IVY70_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957238.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            75152..75340
                     /locus_tag="IVY70_000072"
     CDS             75152..75340
                     /locus_tag="IVY70_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957239.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
BASE COUNT        24620 a        12884 c        16247 g        21610 t
ORIGIN      
        1 tttgcgtaat tatcctccca tcattgaagc aagaagtgtt tttagcgatg aaagaagaat
       61 cagcactatc taatttttct tgaaagtatt gagttgttca tcctaggcag aacgataagg
      121 caagtcttgg tttttattcc ccttgaaaaa gatcgtttaa taaatatgtt ctgtataaaa
      181 atacagtata ttgaaaaata acaatggaag tagaggtgca gagaatgaag aaaagaatga
      241 gaatcatctt agctttaagt gttatgttat tcttcttgac ggcttgtggg caagaagatg
      301 acaaaaaaat cacgctgaac atggcttggt ggggttcgca agtccgacac gatgcaacag
      361 taaaagtaat cgagctttat gaagaaaaaa atccccatgt gacgatcgat tatgaatttt
      421 atgattatga aggatattac acaaaattag gtacactagc agcatcagat gatatctggg
      481 acattttcca actgaacaat caatttcctc aatacatcaa tcagattgaa ccattagatg
      541 aatatgtgga gcaagggata attgatattt ctaatgcaga tgaatcctat ttagcaacga
      601 cgacttacga cgggcaatta gttggactgt ctaacggggt gaattcgttt gcgattgctt
      661 acaataaaaa tatatttgaa caattaaata tccctgaacc agcttcaaat tggacatggg
      721 atgaatttga acaaatcagc tatcaaatca ctgaagaatt aggaatctat gctatttcaa
      781 gattcgaaga ttttatagct ggctgtatta ttcaaatacc acaagttgag aaaggattga
      841 atttttataa tcgggaagat ctatctaaca gcttgggatt tgaaaatcca gactatctaa
      901 cagatttttt ccagctgcgt aaaaatctgg tagacaaagg ggcccatcca gaaccaggtg
      961 gtggagctgc aagtacgacg gatctgtctt ttcaggcggt tagggataga gaagcggcaa
     1021 tggtgtttct atcaagtaac cagttgacag aaatattgtc aggggctcct gaagatatgg
     1081 agattcagtt gattaatcca cctcgcagaa aagcagatgg agaatctggt atccctttac
     1141 gttcttcgca aatgctgagt atggcaaaaa gctcagccca taaagaagaa gctgctaagt
     1201 ttattgattt ctttcaaaac agtgaagaag ccaatgaaat tttaaaagga gaacgtggtg
     1261 taccaatcat gtcgaatatt cgagaacagc tcgctcgtgc agatgatgca actttgcaag
     1321 cgacatttga ttatttagaa atgatttccg atatggacaa cggcgaagtg aatgtctttg
     1381 atagtgcttt gaatccggaa attcaagacc aatacaatct tttagttgaa aaagtcattt
     1441 atgaagaagt aacagcagaa gaagcagcag agagtcttta tgagtttgcg tcaaacattc
     1501 taggatagca gcaggaggaa ggcatgaaaa aagaaaaact aaaacggttc ttggatcaag
     1561 acaatgtggt cggttacgta tttgcttctc cattcatcct aggatttcta ttgataacag
     1621 tgataccgat gatcttgtca ctttattatt cgttcaccga ttatcgtttg ggaaatccag
     1681 tctcatggat cggcttggat aattatataa agatgctgag tgatgcccgt tttttgaact
     1741 ccatccgagt cacttttctc tatgtatttg catctgtacc tgtgaaactg atctttggtc
     1801 tattgattgc ttatctttta acgaggaata tcaaaggaac gactttcttt cgttctttgt
     1861 attatgtgcc tagtttgatc ggtggcagta tttctatcgc tctagtttgg cgggagttgt
     1921 tttctaaaaa cgggttagtg aatattttcc tggaacgatt aggcgcacct gcgctttcct
     1981 ggtttagcga tcaaaaactg gtcatgatcc ctcttgtttt gatgtctgct tggcaattcg
     2041 gttcggcaat gatcatcttt gctgcaggta tcaaagagat cccaggctcg tattatgaag
     2101 cggcagagat cgatggagcg aacaaatggc agacgtttta caagatcacg ttacctcttc
     2161 tttcgccgat cattttgtat aacttggtta tgcagacaat cgcggcgttt atgactttta
     2221 cgcaggcatt tatcattacc caaggtggtc cgaatgatgc cacgaacttt tatgctcttt
     2281 atgtgtataa ccaagctttt tcttttaatt ctatggggta tgctagtgct atttcgtgga
     2341 tcatgctgtt gattatggga gctgtcacct ttctgatttt caaatcatca aaaaaatggg
     2401 tcgttgtgtg aatgagagga ggaagaaatg aataatacag taacgaaatg gtttcaatac
     2461 ggttttattt tgctattagg tctagtcatg atttatccgg tcttatggat gatcgcaggt
     2521 tcttttaaaa cggatcaaga gattatgagt ggttcgctga atttgattcc agagactttt
     2581 caatgggaaa attacgcaaa aggatgggcg gggtttgctg gaatttcatt ttttactttc
     2641 tttaggaatt cgtttgtcat ctctgcagtt tcgacgatag ggacagttct gagctcgact
     2701 tgtattgctt acgcattatc gagaatcgat ttcaaaggaa aaagattctg gttcgtcgtc
     2761 atgttagtaa cgatgatgat tcctgcacaa gtgattttga ttccgcagtt tattatttac
     2821 aatcggctga atttggttgg gacgtatgtg ccgctgattt taccacattt ttttggacaa
     2881 gcatttttca tttaccagat catgcaattc atggtcagca tccccaaaga gttagatgaa
     2941 tcagcgatta ttgacggatg cagcaagtat agtgtcttta ccaaaattat ttttcctttg
     3001 ctgaaacctt cgatcatcac gacgatcatc atccagtttt attggaaatg ggacgacttc
     3061 atgggaccat tgatttattt aaacaagccg agatcttaca cggtatcgat tgccatcaaa
     3121 ttatttgcag acgctggatc tagtacgact tacgcgagta tgtttgcgat gtctacgttg
     3181 tcgctgctac cggtttttct gattttctta ttcttcaata aatatcttgt ccaggggatc
     3241 agtacaagcg gtctgaaggg ctgaatatga aaaagccaat agaagagatg tttctttaca
     3301 agctcaacta ctatgtctgt cggatttttt taaccagtct tatcagtaat gctcttttcg
     3361 ctttagttct aggatgtttg ttcactttag gattagtgct gtctgctcgt gttgtctatc
     3421 tgatttattt tttgatgatg tatccttctt attatctatc tgtccattac ttggcagaaa
     3481 aaaaggtatg ttggaggaaa ggaaaaatca aacggatgtg tttcatcatt acgttgtttc
     3541 agctgtttgt cattattttt gcagttgatt ggtatttatt agtgggaaag attggagctt
     3601 ggtatctgat tccttttcta ttgtgtctgt tgctttttgt atcccatagt ttttttagta
     3661 cgctaattga tatctgcaaa gaagataaag cagatagtct tgtttatttg atcaaaagga
     3721 atatctggct gttgtcaaaa aacgtcaagg ggtttctgtg gtatttatgc cttttgtatg
     3781 gactgttctg gtgtatacag catgattttt tactcactgc attactgtat ccagggctgc
     3841 catttgtcac agtattttgc ttaaaagaaa aggagaatga ctttaaatga agaaaaaata
     3901 tgtacaagtc ggacttggtg ggagagcgcg ttttttttat gaggcaattg cctctacgta
     3961 tcatcaaact tgtgaactag tcggtttttg tgatgtatct caaacaagga tggattatgc
     4021 aaatcggata cttgtgaaca agtatggaca gaaaaaagta aagacatgta tgccatcaca
     4081 atttgagcaa ctactggatg acgtacggcc ggctgaagtc attattacgt cagtagatcg
     4141 aacgcatcat gattatatca ttcgtgcaat gaaaaaagga tatgatgtta tttgcgaaaa
     4201 acctctgact atcgatgagg aaaaagctca agaaataata acggtccaaa aacagacggg
     4261 aagaagcctg cgagtcactt ttaattatcg ttatgcaccg tatcacacga agataaagga
     4321 actgatagaa agtgatgtga ttggtgatgt gttttctatc cacttcgaat ggctgttaaa
     4381 tacgcaacac ggggcagatt atttcagaag atggcatcga aataagcaca atagcggcgg
     4441 gcttcttgtt cataaatcta cccatcattt tgatctagtg aatttttggt tgaacagccg
     4501 tccccaaaag gtatttgcat tcggtggatt aaacttttat gggatgcata atgcggagaa
     4561 acgcggagta gaaatatttt acagtcgatg ctatggaagc gaactagcta aagctgatcc
     4621 atttgctata gacctgtcag ccaatgagga attgcgagaa atgtatttag aagcagaaaa
     4681 agacagtgga tatattcgtg accgcagtgt attttccgat gatatctcta ttgaagatac
     4741 actaggcgta acggtcaaat atgaaactgg tgcgattttg acgtattcgc taaacgcgta
     4801 tatgccgtgg gaaggattca atgtttcttt caatggcagc aaaggacgga tcgaaatgaa
     4861 agtgatcgaa aagtcttaca tcaatgcagg tggagataaa aaggatgaag gagcagccgt
     4921 tcttcagtcc atccatgtct atccgatgtt cggtgagcca tatgaggtaa tcgtcgaaca
     4981 agaagcgggc ggacatggcg gaggagatcc gcgtttatta gatgatctat ttggtgaaga
     5041 aaaagaagaa gatccgtgga atcgggcagc aactcacgta gatggtattt tgtcgatttt
     5101 gacaggaatc gcagcaaatc attctattgc ttcaggaaaa gcagtagtga ttgacgagct
     5161 tgtcagctta aaagaataag aatagattta cggaaattac ttcccgtagt ttataaaatc
     5221 ttgccttatt gccgataggc tgtcttttga acactattta ttcggaaaaa ggggctatga
     5281 catgcttttt gtcatgcctt ctttttatgt gggctgcttt tttagatagg agaaaataaa
     5341 aataactatt taaacccgaa caaaaaggaa taatgcatta aaaatcatta gattttacaa
     5401 aaaaataagt aaaccacgtt gtcagtttca gaaagtagtg ttagactgtt ttcaatattt
     5461 gaaacaacat tttttattca aatatggcaa actgccttca cgtgcaaaac agttgcaaga
     5521 aaatgaattt acactattat tagttttaca tacctactta tatatcgtca taatatggat
     5581 gacagtttct agccagtacc gtaaatgctg gactataagt aaaagattgg ctatttcacc
     5641 cttcttttct tatgataaag tggagggtgt ttttctttaa cagaaacata gtaggttttt
     5701 gcacaaaagc ggttatttcg gtattaataa atgataaagg agcaaataaa gtgaaagaat
     5761 tagttgaacg tattcaaaaa gacggacgta tattaggcga gggcgtatta aaagtcgaca
     5821 gcttcgtcac tcaccaagta gaccctgtat tgatggaaca aatgggaaaa cggtttgcag
     5881 aagtatttaa agaacaagga atcacgaaag ttgtgaccat tgaagcttct gggatcgcac
     5941 cagctttatt tgctgcaaga gaattagatg ttccaatgat cttcgctaga aaagcaaaaa
     6001 gtttgacgat ggatgaagaa ttattgacag cttctgttta ttcttttacg aaacaagtga
     6061 caagtacgat ttctatttct cgtaaatttt tatcttcaga agacaaagta ttgattatcg
     6121 acgatttctt ggcaaacgga caagcggcaa aaggattgat cgaattatgc cagcaagctg
     6181 gagcgcaagt agaaggaatc gggatcgtca tcgaaaagtc tttccaagat ggacgtcagt
     6241 tacttgaaga aatgggattg agagtcgttt ctcttgcgcg tattgcttcc ttgacgaatg
     6301 gacaagttga gtttcttgag gaggacgcat aatcgtggaa aaagaaacac aaaatggaaa
     6361 agctgcggtt ttagggctgc agcatctgct agcgatgtat gcaggagcag tagcggtgcc
     6421 tcttttgatc ggtacaggac taggctttaa tcaagaacag atgacttatt tgatttcaat
     6481 tgatatcttt atgtgtggat tagcgacatt attgcaattg acagttaacc gatttttcgg
     6541 aattggcttg cctgtcgttt taggatgtgc gatccaagca gtagctccac tgatcttgat
     6601 cggaaccaat gaaggagtag gagctatcta cggatctatt atcgcttctg ggatctttgt
     6661 tgtgttagtt tctggtgtct tttctaaaat taaaaaacta ttccctccta tagtaacagg
     6721 aacggttatc accgttattg gtttgacttt gattcctgta gcaatcgaga aaatgggtgg
     6781 aggtaatgca acagcagctg attttggtga caaaacaaat ctgctacttg catttgtgac
     6841 gattctccta attgtaggcg tgcaaatgct ggcaaaaggg ttcattcgtt caatcgcggt
     6901 attgattggt ttagtcggag gaagcatctt agctgctttc atgggaatga ttgatctgag
     6961 cgcgattgga gatgcgccgt tgttccatgt tccacaaccc ttttattttg gtaaaccaac
     7021 tttcgacgta tggtcgattt tactaatgat catcatttcc atggtcagca tggtagaatc
     7081 aacaggtgta tattttgcac ttggtgacat tactgggaaa aatgttggag aagaagaatt
     7141 gaaaaaggga tatcgtgcag aaggacttgc cgttatctta ggcggtattt tcaatacatt
     7201 cccttatact ggcttttctc aaaatgtcgg attggttcaa ctttcgggaa tcaaaacaag
     7261 aaaacccatc tatttctctg cattcttctt gattgtttta ggattattcc ctaaaatcgg
     7321 tgcgttagca caaatcatcc ctgagccggt acttggtgga ggaatgttag tcatgttcgg
     7381 gatggtggca gtccaaggaa tccgtatgct gttggaagtt gatttcagca atgataaaaa
     7441 tctattgatt gttgctgttt ctgttggatt tgggctgggt ttcaatgtta cgccgacatt
     7501 attcagtcaa atgccgcaaa cattgcaaat gttcactgga aatggcatcg taatgagtag
     7561 cattacggct atcatattga atttgatctt taatggattg aaaaaagatg aagaaatatt
     7621 ggaagaagcc ttcaagaagt aatagagcaa aagaaaggtt gtgaaatcag cgttttgacc
     7681 tgattgttag agaaacaaac ggaaaaatag ttcctcgtat tttgccgttt gtttgagcta
     7741 ataccgaggg tcagctgatt gaacaccgtt gaaggaagag gtcgtgagcc tgccgttcat
     7801 ctccgagtca caaggaacaa tttcaaaaaa aagcttctca tgtttgttga atattgttcc
     7861 ttgtgaccga ggagttggca ggagaacacc gtttatccat aaaaaggaat gtgacctaac
     7921 ttttgtcaca ttcctttttt ttatctttaa taaacacgaa caataaaatg tttttctttg
     7981 ttaacattcg aaatttcttt gacatcaaaa aaagtgattg ttaaaatatg aatgtagaaa
     8041 aactgggagg aaaaacaatg acagcaaaaa tctctgtgat tatggggagt acttcagatt
     8101 gggaaacaat gaaagaagca tgtcaaatat tggatgaatt cggtgtgtct tatgaaaaaa
     8161 aggtcgtttc tgctcatcga accccagatt tgatgtttga atatgcacaa aacgctcgaa
     8221 aaaaaggaat caaaatcatt attgcaggag cagggggagc cgcgcatctg ccgggtatgg
     8281 tcgcatcaaa aacaactttg ccggttatcg gtgtaccggt ccaatcacga acattaaatg
     8341 gtttggattc gcttctttcg attgtccaaa tgcctggagg agtacctgta gctactacag
     8401 caatcggaaa agcgggagca attaatgctg gactacttgc gatacagatg ctttccatgt
     8461 atgatttaga attggaggct aaattggctg aaagacgttc aatgttggct aaaactgtta
     8521 tagaaagtag tgatcaactt ggttaaacct ttgttgcctg gttcaacgat cgggatcgtc
     8581 ggtggcggtc aattaggtcg tatgatggcg attagtgcaa aagaaatggg ctttaaagta
     8641 ggtgtgctgg atcctgtgag cgattgtcca gccgcacaag ttgcagattg gcacattgaa
     8701 agtgtctatg atgacacctt tgcgctgaaa gagcttgcca gaagaacaga tgtcattacc
     8761 tatgaattcg aaaacgtcag tgtagaggcg ttaaatgcga ttttacccat gtcatttatt
     8821 cctcaaggga cagacttgct tgcgattacg caagaccgcc tgttagaaaa atcttttttg
     8881 gaaacgaata acatcgtgat tgctccttat gcaacgatcg tcagtccgac ggatatccaa
     8941 gatgcgattg acgggatcgg ctatccttgt gttttgaaaa ccactcgagg cggctatgat
     9001 ggcaaggggc agtatgtttt gaaaagtcgg gcggatctag ctcctgccat ggacctttta
     9061 agagaaggaa cctgtgtttt agaagcatgg atcccgtttg aaaaagagat ttcgatcatg
     9121 gtggcaggaa atggtcaagg agatttcacg acttttccgg tcgtggaaaa catccaccac
     9181 aacaatattc ttcatgaaac gattgctcca gccgcgattg atcaggatgt gatcgaggag
     9241 gctgagcgta ttgcccgtgt gattgcagaa gcagtgagcc tttcaggtgt tttaggggta
     9301 gaaatgtttt taacaaaaac aggcgggcta tatgtgaatg aattagctcc tcgtcctcac
     9361 aattctggac attattctat tgaagcttgt tctatgagtc agttcgatgc ccatattcga
     9421 ggtatttgtg gttggccatt aggagaaaaa gtacgtttga taagcaatgc agtaatggtc
     9481 aatgttttgg gagaacaact gctggaaagc tatcgcttga ttgagaataa gccagactgg
     9541 caattccatt tctatggaaa aaaacaagca aaaaaaggtc gcaagatggg acatatcact
     9601 atcttgacag aagacatcca gcaaacgctg gaagaaatca aacaaacgaa tatttgggat
     9661 tagaggagaa aaaacatgtt agaacgttat acacgccctg aaatggggaa tatctggacg
     9721 gaagagaaca aataccgtgc gtggctcgaa gtcgagattt tagcagatga agcttgggca
     9781 gaacttggtg aaatcccgca agaagatgtg aaaaaaattc gtgagcatgc atcatttgat
     9841 atcgatcgga tcttggaaat cgaaaaatta acacgccatg atgtcgttgc gtttacacga
     9901 gcggtttctg aaacattggg agaagaaaga aaatgggtcc attatggtct aacaagtacg
     9961 gatgtcgtag atactgctta tggctatctc atcaagcaag caaatgatat tttacgggaa
    10021 gacttgaaac gttttacgga aatcattggc gaaaaagcaa aagaacacaa atatacagtg
    10081 atgatgggtc ggacacacgg tgtccatgct gaaccaacta cttttggatt gaaacttgcc
    10141 acatggtatt cagaaatgaa acggaacatc gaacgatttg aacatgcggc aaaaggagta
    10201 gaagcaggaa aaatctcagg agcagtggga acgttcgcaa atatcccgcc atttattgaa
    10261 gagtatgtat gtaaacaatt ggggatccgt ccacaagaaa tttctactca agttctccct
    10321 cgggatctac atgctgaata tttctcagcg atggcgctta ttgctacaag tattgaacgt
    10381 tttgcaacag agatacgcgg attgcaaaaa tccgaaacac gtgaagtaga agaatttttt
    10441 gcgaaaggtc aaaaaggttc ttctgctatg ccgcacaaaa ggaatccgat cggttcagaa
    10501 aatatggctg gtctagccag agtggtgcgc ggacacatgg tcactgccta tgaaaatgtc
    10561 acattatggc atgaaagaga tatttctcat tcttcagcag aacgaatcat cttgcctgat
    10621 accacgattt tattgaatta catgttaaat cgttttggca atatcgttaa aaatctaacg
    10681 gtctttcctg aaaacatgaa gcggaatatg tctgcaacat ttggtttgat ttatagccag
    10741 cgcgttatgc tgaaattgat cgataaggga atgacaagag aagaagctta tgatcttgtt
    10801 cagccaaaaa ctgcgtacgc ttgggacaac caagtgcagt tccgcccgct gttagaagaa
    10861 gatccaacca tcacatcggt attatctaaa gaagaaatag acgatgcctt tgattatcat
    10921 catcatttga aaaacgttga cgtgatattc aaacgagtag gtttggaata aagaagagtg
    10981 agttgcaaaa ttcaattaaa atagaatagc aaaaataacc aaaaaagaag cagatccgtt
    11041 agcggttctg cttcttttag tttattatat tttcttatca ataataggaa cctgatttta
    11101 aaagcggcta cctctttacc gtgcctcttt acatacaaaa aatagaggaa ttaagcgtaa
    11161 atggcttggc tattataact aaaatgctgg ttatggagat gttgcagcaa gttttcgaga
    11221 aaagcgtatt ccatctcttg tccattgaat aagatctgga tacgataatg atttttgaaa
    11281 cgcttctgtt catgaatctg actgattgaa agttcgcctt tgtgttctgt ctgataaaga
    11341 aaagtccttt tacggcattc agcacagcgc ttggttgctc ggcggatttt tcggaatgta
    11401 taaaaactag tatctttatt gcataaataa acgtctttga tcgacagcaa atcattatat
    11461 agacaggaac gttcttcagc tgttaattct gataaaaaaa gctgaccttg ttttattgca
    11521 tcttccatcg gcaagtgcct cctcgaaccc attatccaaa tttttagtta attctaatat
    11581 accatcatta agtataaatg taaacgcttg ttgatggtat aagattttcg tttctgataa
    11641 aacacgaaca ttaaaagaat ctaatgcctt aaatgtacat atttttgttg atttataaaa
    11701 agatgattgc taaaatttaa agtgaaaaaa cgaacaaaaa acgatgtatt tgcttttaat
    11761 gtaaataaaa aggagagacg tacatggaga agaaagaact tttgtatgaa ggaaaagcca
    11821 aaaaaatata tcttacttct gatgagaact tgatttggat agaatattta gatcaagcaa
    11881 ctgcgttgaa tggcgcgagg aaagaccaga taagtggaaa aggagaattg aataatcaaa
    11941 tcacaagtgc aattttccgt tatctgactc aaagaggaat aaaaaatcat tttattgaac
    12001 aattgtctaa aaccgaacaa ttagcaaaaa aagtacaaat tatccctctg gaagtggtta
    12061 tccgaaatat tgcagcagga agtttctcaa aacgtctagg tgttcccgaa ggtaaagaat
    12121 tcatcaaacc aatcattgaa ttctactata aggatgacca gttggatgat ccatttatca
    12181 atgaagagca tatctatttc ttacaacttg caaagccgga agaaatcagt gagctgaaag
    12241 cccaagcgtt ggctattaat gaaattttga ccgaattatt tgctaaggtc ggtttggttt
    12301 tagtagattt caagctagaa tttggccggt cgaatggaca aattttgctt gcggatgaag
    12361 taacaccgga tacttgccga ttatgggatg aacgtacgaa tgaacatatg gacaaagacg
    12421 tttaccgcag agatctaggt gatttgatcc cagtctatca agaagtatat aaccgtctat
    12481 cggaactaac tgaagaaaag gagcgagtat gatgtatttc gtaaaagtat atgtaaccta
    12541 taaagattca gtgttagatc ctcaagggga ggcagtaaaa aaagcagtcc atcgtatggg
    12601 atatgaaaca atcgaagata tccgaatagg aaaatacttt gagatacaac ttgctgatgc
    12661 agaagaacca atcgatacag tgattgaaac gatttgtgac aagctgcttg caaatgtaaa
    12721 tatggaaaca taccgatatg aaatcaaaaa aatggaggaa gtataacatg aaatttgcgg
    12781 tcatcgtttt tccaggttca aattgtgacg cagatatgtt atgggcagtt cgcgagatca
    12841 tgggcgctga tgccgaattt gtacggcatg atgagtcctc tttagaagga tttgatgggg
    12901 ttttactgcc gggaggattc tcctatggtg actatttgag atgcggtgcg attgctcggt
    12961 tttctacaat catggaagaa gtgattcgtt ttgctgaaga agggaaacca gtattcggta
    13021 catgcaatgg attccagata ttgacagaag caggactttt gccaggtgta ctgagacgaa
    13081 atgagtcttt gcattttatt tgcaaaactg taccattgaa agtcaacccc aagacggcct
    13141 ttacttcaga gtatgaagcc gacgaaataa tctatcttcc agttgctcat ggggaaggaa
    13201 attatttctg tgacgaagaa acacttcaag aactaaaaaa aaataatcag attgccttta
    13261 cttataatga caacatcaac ggcagcgtgg aaaacattgc agggattacg aacaaagcag
    13321 ggaatgtgtt aggaatgatg cctcatccag aacgtgcagt agaagacttg ctaggatcag
    13381 aagatgggaa acggttcttt gcttccattt taaaaaactt cggaaaggtg accgtataga
    13441 tgaaaacatt gaaagaaccg acagctgaag agatcaagaa aaaaagaatc tatgccgaat
    13501 ggggattgac tgatgaagaa tatcgaatga tcgaagaaga tatcttagga agaatgccta
    13561 attatacgga aactggactg ttttctgtta tgtggagcga acattgttcc tataaaaatt
    13621 ctaaaccggt tctacgcaaa tttcctacta gtggacctca tgtgttgcaa ggccctggag
    13681 aaggcgcagg catcgtcgac atcggtgatg gactagcagt tgtcttcaaa gcagaaagtc
    13741 acaatcatcc ttcagccgtg gagccttatg aaggagctgc gacaggagtt ggcggaatca
    13801 tcagagatat tttcagcatg ggggctcgtc caattgcgtt gctagattca ttgaggttcg
    13861 gcgaattaga taatccaaga acgaaatatt tactagaaga agtagttgca ggtatcggcg
    13921 gatacggcaa ttgtatcggc ataccgactg taggcggcga aattgcattt gatccttgtt
    13981 atgaaggaaa cccattagtc aatgcaatgt gcgtaggttt gattgaacac aaacatatcc
    14041 agaaagggca agccgctggc gtcggtaatt cgatcatgta tgtaggtgca aaaacaggtc
    14101 gcgacggtat ccacggagca acttttgcct cagaagaatt taatcaagaa gaagaacagc
    14161 aacgttctgc tgtacaagta ggcgatcctt ttatggaaaa gctgctgctt gaagcgtgtt
    14221 tggaattaat cttgcatcat tcggattctc taataggtat acaggatatg ggagcagcag
    14281 gacttgtttc atctagtgcg gaaatggctt caaaagctgg ctcaggactt atattgacat
    14341 tagatgaagt cccacaaaga gaaactggga tgacacctta tgaaatgatg ttatcggaat
    14401 cacaagaacg gatgttgatt tgtgttaaat caggagagga agaaaaaatc caagaactat
    14461 ttcagaagta tgatctagat gcagtcacta tcggtaaagt aacagacgac gggcaatatc
    14521 ggctatatca tcaaggagaa gaagtagcaa atctgccagt cgatgcatta gctgaagatg
    14581 caccagttta tcataaagaa atgaaagaac cagctcgaat cgtagaattt cagcagttag
    14641 ctccttatca gccagttatc gaagaacctg gacaagtatt gcttgatctg ctgcagcaac
    14701 cgacgattgc ttcaaaacgt tcaatctacg aaacttatga ttcacaagta caaaccaata
    14761 cagttgtccg tccaggaagc gatgctgctg tgatgagggt tagagggacg aataaagcgt
    14821 tagctatgac cacagactgc aacgcgcgct atctgtattt ggatccaaaa atcggaggac
    14881 aaatagcagt ggcagaagct gctagaaata ttattgctag cggtggtaag cccttggcga
    14941 tcaccgactg cctgaactat ggttctcctg ataaaccaga agttttttgg gaattatcga
    15001 cttcagctga cggaattgct gctgcgtgtg aaacgttagg aactcctgtt atttcaggaa
    15061 atgtctcttt atataatgaa acggatggtc aagcaatcta tccaacacca atgattggta
    15121 tggtcggatt gattgaggat cataaacata tcactactca ggaattcaaa aaatcagggg
    15181 atctaatcta tatattaggt aagacgtttg cagattttga tggcagtgaa ttacaaaaga
    15241 tgcagcttgg aagaatcgaa ggtgtgatcc gaaactttga tctatccatt gaaaagagaa
    15301 accaagagtt agtattgaca gccatccaaa acggattgat tgaaagtgct catgactgtt
    15361 cagaaggtgg cttggcaatt gcactggcag aatcagcttt caaacatcag ctagggatat
    15421 ctgtacagtt tgaattgtcc tctgctcaat tatttgcaga aactcaatca agatttgtgt
    15481 tgacagtcgc accggaaaat aagacacgat tcgaagaaat gatgggagat gctgctgttt
    15541 tagcaggtaa ggtgacggat gaagcgataa tcgaaatttc agcaactgac ggtcagatca
    15601 aaatagaaac tgcagtagca agaaagtgct gggaggatgc aatcgtatgt cttatgaagt
    15661 gaagagttta aatgaagaat gcgggatctt cggtgtttgg ggtcatccag acgccgctag
    15721 tgtggcatac ttcgggctcc atagtcttca gcatcgagga caagaaggtg caggtatcgt
    15781 gtccaatgat cgaggaaagc ttaaggggca tcgagatttg gggctgcttt ctgaggtatt
    15841 caaagatcaa agaaaattgc agcagttaac aggagaagct gcaatcggac atgtacgtta
    15901 tgcaacagca ggcaatggaa gtgtggacaa tattcagcct tttctattca aatttcatga
    15961 tcagtcgatc ggcttagcgc ataatggtaa cttgatcaat gctcgttccc ttagaaaaga
    16021 gttagaggaa aatggggcga tcttccattc taactccgat acggagatat tgatgcattt
    16081 gatccgacga agtgaagaaa cagatttttt aaataaatta aaaagtgctt tgaatgaagt
    16141 caaaggtggc tttgcctatt tattaatgac agaaacggca atgattgccg ctttggaccc
    16201 aaatggtttt cgtccattag caatcggaca aatgaaaaac ggagcatacg tgattgcctc
    16261 ggaaacttgt gcattagaag ttattggggc acgttttata agagatgtcc agcctggtga
    16321 gatagtgatc atcaatgacg aaggaatcca gattgattgc tttacgaagg acacgcagct
    16381 ttctatttgt tcgatggaat atatctattt tgccagacca gattcaaaca tcgcagggat
    16441 caatgtgcat actgcacgga aaaatatggg acggaattta gcgaaagaat cgccagtaaa
    16501 agcagatatg gtcgttggcg tacctaattc ttctttgtca gccgcaagcg gttatgcgga
    16561 agctagtgga atcccttatg aaatcggatt agtaaaaaat caatacattg cccgaacatt
    16621 tatccagcca acgcctgagc taagagagca aggtgttcga atgaaattat ctgctgtacg
    16681 aggagtagtg gaaggtaaaa aggtgattat ggtcgatgat tcaattgttc ggggtacgac
    16741 aagcagacgg atcgtccatc ttttgaaaga agcaggggca aaagaagtcc atgtgaggat
    16801 tgcttctcct cctttgaaat atccatgttt ttacgggatt gatattcaaa cgcgtaaaga
    16861 actcattgcg gcaaatcaca cgattgatga aatcaaagaa tgtatcggtg cagattcttt
    16921 aggtttttta agtgaagagg gtctgatcga atcgatcggt ctggaaaaag atgctccgta
    16981 ttcaggattg tgtatggctt atttcaacgg agattatccg acgccgttat atgactatga
    17041 agaaaaatat caggaatcac taaaagaaaa agtatcgttc ttttaaaagc agaccaagaa
    17101 gggagtatat gaattcaatg gcaaatgcat acgcaaaagc tggcgtagat gtcgaagctg
    17161 gatatgaagt agtagaacgg atcaagaagc atgtcaaaaa aacggaacgt ttaggtatga
    17221 tgggcgctat aggaggattc ggcggttgtt ttgatttatc ggtctttcaa ttaaaagagc
    17281 cagtccttgt ctcggggacc gatggcgtgg ggacgaaatt agcagtggca atcgaagcag
    17341 acaaacacga tacaatcggg atcgactgcg tagcgatgtg tgtcaatgat atcttagcac
    17401 aaggcgcaga gccgttatat ttcttagact acttagcaac ggggaaaaat gatccgttac
    17461 gtttggaaaa tgtagtaaaa ggtgtagcag atggatgtgt tcaaggaaat atggcgctga
    17521 ttggcggaga aacagctgaa atgcctggta tgtactcagg taatgattat gatctagccg
    17581 gatttgctgt agggatagct gaaaaatctc aattattgac aggagaaacg atcaacccag
    17641 gagatgtgtt gcttggaata gcatctagcg ggattcattc aaatgggtac tctcttgttc
    17701 gcaaaatttt ctttgagcag cagcagtttt ctttatcatc aaaactgcca gaattgaaaa
    17761 aaacacttgg cgaagaattg cttgagccaa cgaaaatcta tgtttcttcc ttgcttccgc
    17821 tgatcaaaga aaagaaaata aaaggagctg cacatatcac tggtggaggg tttgtagaaa
    17881 atatcccgcg aatgttacca gaatctatgg ctgcacaaat caaacttggc tcatggccga
    17941 ttcttccaat attcggctgt ttacaaaaat atggtgaaat tcctgagatg gagatgtatg
    18001 aaatcttcaa tatgggcatc ggtatggtcc tggcagtcga agaatcgaaa gtaaaagaaa
    18061 tagtacacga aattgagcaa gctggcgaaa aagtttatga aatcgggaaa gtgatcgcca
    18121 aagaaaaaca tcgtgtggtg tttataggag aagaacgatg agaatcgctg tttttgcttc
    18181 tggaaacgga agcaactttc aagcgctagc cgattatcta tcgaaaaaag gattggaatc
    18241 ttcgattgac tggttgtttt gtgatcagcc tgaagcatat gttttgaaac gtgcaactgc
    18301 gttgagtgta ccggcagatt gtttttcacc taaagaattt gattcaaaaa aagagtatga
    18361 agaagcaatt ttgcataaat tgaaagaaaa aaagatcgat ttgatcgtat tagctggata
    18421 tatgcgaatc attggaccgg tattgctgaa aaattatgat aaaagaatca tcaatatcca
    18481 tccgtctctt ttacctgcct tccctggttt gcatggcata cgtgatgcct ttgaagctgg
    18541 agtaaaagaa acgggtgtca caattcatta tatcgaccaa ggtgtagata ctggaccaat
    18601 catccggcag gaaaaagtga gaattgaaca ggaggacacg ttcgattctt tagaagaaaa
    18661 aatccataga gtagagcatc gaatctatcc agaagtgatt agtgaaatta ttgaaaatgg
    18721 aggctatctg aaatgacaag agcattgatc agtgtatcag ataaaactgg gattgtagca
    18781 ttcgcccaag gtttgattga acaaggagta gaaatcattt caacaggagg aacaaagcat
    18841 gcgttggata aagctgggat caaaacgctt tcgatcgaag aagtgacagg gtttcctgaa
    18901 atgatggatg gccgtgtcaa aacacttcat cctaaaatcc atggtggtct tttaggaaga
    18961 cgcgatctcc ctagccatat ggaggcaatg gaaacggaaa atatccaacc aatcgacttt
    19021 gtttgtgtca atttatatcc tttcaaagaa acgattatga aaccagatgt cgaaatagca
    19081 gaagctattg aaaacattga tatcggtggt cctagtatgc tccgtagtgc agcaaaaaac
    19141 catgaatttg tcacctctgt tgttgatcca tctgactatg aagaagtact ttcagaatta
    19201 cgtcagtatg ggcatacttc actttctttc agacaaaaac tagcggcaaa agtctttcgt
    19261 catactgcgg catatgatgc actgatcgct caatacttaa cggattgggt ggaagaaaaa
    19321 gaaccggaaa aattgacact tacgtatgaa cgaaaacaaa cgcttcgtta cggtgaaaat
    19381 agccatcaag ccgctgcctt ttatcagtcc gctcttctgg aaaaatattc gatcgcagca
    19441 gcaaaacaac tccatgggaa agaactttct tataataata tccgagatgc agatgctgcg
    19501 ctacggatca tgaaggaata tgacgagcca acggtagtcg ccttgaaaca catgaatcct
    19561 tgtggtattg ggacagcatc tacgatttta gaagcgtggg aagcagcgta tgaagcagat
    19621 cctgtttcaa tctttggtgg gatcatcgtt ttgaatcgag aagtagatct gccaacagca
    19681 gaacagatgc ataagctgtt tctagaaatc atcattgccc cttcttatac acaagaagct
    19741 ttggaattac tgcaaaaaaa gaaaaacatc cgattactga cagtagactt tgcgcaggaa
    19801 gaaaatcaaa atcgagaaga aacggtctca gtcttaggtg gacttcttgt acaagatcaa
    19861 gatctggcaa tagaaaatga agaagagtgg aaagtcgtga caaaacgtaa gccgactcca
    19921 gcagaagcaa aagcattagt gtttgcttgg agagctgtca aacatgtgaa atctaatgcg
    19981 atcgtactgg caaacgaacg tcagacagta ggaatcggag ccgggcagat gaatcgagtc
    20041 ggttcagtaa aaattgcgat tgaacaagcg caagagaaaa tggaaggtgc agttttagct
    20101 agcgatgctt attttccaat ggatgattcg gttgaatatg cagccaaaca tggtataaaa
    20161 gcaatcattc agccaggagg aagcatcaaa gaccaagatt ctattgatat ggcgaataaa
    20221 tacgatctag caatggtctt tactgatgtt cggcatttta gacattagag aggagtcgga
    20281 taaatgaaca ttttggtgat cggtgcaggt ggtcgtgaac atgcaataag taaaaaactt
    20341 ttagccagtc cgaaggtgga tacggtgtat tgtgcgccag gaaatccggg gatgacaaaa
    20401 gatggtatca attgtgtcgc tatcagtgag cgagatcatt cagcgttaat ccattttgtt
    20461 aagaaagagg ctattgattg gagtatcgtt ggtcctgaag ttcctttgtt aaatgggatc
    20521 gtcgatgatt ttatggcgga aggattgaaa atcttcggtc cgacaaaagc agcagctctg
    20581 atcgaaggtt cgaaaagttt tgctaaacaa ttaatggata cttatcaaat cccgacagct
    20641 aaatccagaa gttttgaatg ttacgaaaaa gcagcagcgt atatcaaaga aaatggtgcg
    20701 ccaatcgtag tgaaagcgga cggcttagca gctgggaaag gtgtagtcgt ggctaccaca
    20761 gtgaaggaag cgctggatgc ggcagaacaa atgttgaacc agcatcgatt tggggatagt
    20821 agcaaaaaaa tcgtcgtaga agagtttttg gcaggagaag aattctccct tttggcgttt
    20881 gtccgtaata ccaaggtgta tccgatggtc atttcgcaag atcataagcg ggcatttgat
    20941 aacgataaag ggccaaatac aggcggaatg ggtgcttatt caccagtacc gcaaatttca
    21001 aaaaagatga ttgaaaaagc tgtggaagaa gttttaaaac cagcggcttt gggaatgaaa
    21061 gaactaggac gctctttcac gggtgtttta tatgcgggtt tgattgctac gaaggaagga
    21121 ccaaaagtga tcgaattcaa tgcgcgtttc ggtgatcctg aaacgcaagt cgttctttct
    21181 cggttgaaga gtgactttgc gcaagtaatc gatgatttat tagagaatcg tacagtagag
    21241 ttaaagtggc agcaagaagg atacaatgta ggcgttgtgg ttgcagctgc cggttacccc
    21301 gaagaatatg agacaggaat ggttctacca gattttctgg aagaagagct ttctgtttac
    21361 tatgctggag tttcctcaaa taacggcaaa ctagttggag caggaggacg tctttattta
    21421 gtagaagcat acggtgagac gatcaaagaa gcacagaaaa aaatttacac cgcgttaatg
    21481 gataaaacga cagatggaac attttatcgt acagatattg gagaaaaagc tgccaatagc
    21541 gaactgatcg tgaagtaaat cagctgcttc catttataaa aaatacaaca aacataatcg
    21601 gaagcgccga ttttagtgaa gccttttcat aaagagtgtg gtacactttt gttgtgtata
    21661 taaataagga ggagttcaga tgaaaaaaat tcaattaggt acttctgatc agaaagcagc
    21721 tgcagtgatc ttaggctgta tgcgtatcaa caatgcggaa gatccagtaa aagtgattga
    21781 gacagcttat gaaaacggca ttgatttttt tgaccatgca gatatttatg gaaacggtga
    21841 atgtgaagaa atctttgcag atgctctagc aaaaacatca atcaaacgag aagatctttt
    21901 tattcaaaca aaatgtggaa tcgttccagg tgtgatgttt gatttttcta aaaaacatat
    21961 cattgaatct gtagaaaaaa gtttgacccg tctgaaaatg gattatgtgg atgctttact
    22021 tcttcatcgt ccagatacat tggtagaacc agaggaagta gctgaagcgt ttgatgaatt
    22081 ggaacggtca ggaaaagttc gttattttgg cgtcagcaat caaaaaccaa tgcaaatcga
    22141 attgctgaaa aaatatgttc gtcaaccatt agtagctaat cagctgcaat ttggcttgaa
    22201 acatactgga atgatcgatc aagggattca tgtcaatatg acagatgaag caagtatcga
    22261 tcatgacggt agtgtacttg attattcccg attgcatgat atgacgatcc aagcatggtc
    22321 accataccag tatggatttt ttgaaggtgt atttatcggg aacgaaaaat tccctgaatt
    22381 aaaccaaaca ttagaagaac tagcagctaa atatcaaacg acaccaactg gcttggcttc
    22441 tgcttggatt ttacgccatc cagccaatat gcaagtgatc gccggtacga tgaacgtagg
    22501 gcgaatcaag gaaatcgctc aagcttcaga aattaatatg tctcgggaag attggtacga
    22561 attgtaccgt gctgctggaa acattttacc ttaaagattg tcataagcag gtcaaaaggg
    22621 ctataaatta aaaagacaga ataacttgaa agctgttttc agcttcgaga tattctgtct
    22681 ttttttattg caaatggaaa gagtatcatt gtacggattt cgtttattag ttatttttgt
    22741 ttttaaggaa aagctgtgtc cgatattctt ttggagaaat cccttgtttt ttcttaaata
    22801 aataactgaa atagttaggg tcattataac caatttcaaa agcaatatct gaaatacgat
    22861 agtctgattg ctgcaataat ttttttgcta attcgattct ttgttctgtc agataatcga
    22921 tgaatgtttg gttaaacgat tgagaaaaaa tcgtactgaa atgagaggga cttaatgcaa
    22981 cttgttgagc gacccaagtt aaggacatat ctggatcggt aaaattttct ttgataaaat
    23041 ccaatgcatg gttcaaaatt ggctgatatt tgttccttga aggctgtgag ggcagagcag
    23101 ttaagaaaga aatgaacata taaactgtcg aacggtaaag cgagtcgtcg gcgctgattg
    23161 ctaagcgttg actggtggat gtgcaatgaa ccatttcttg cgggattttc ttttctttca
    23221 ggcagacatg ctggagtagc ttagtcaaga caaataaacg atataatcgt gttcgagttg
    23281 gtgtgccttg tggtttcgtc aagtgttcga tgtacttaga tacttcgttt ttcgataatg
    23341 aagacaaatc aagtgagagg tcaaagtaaa agacagaatc aactggaagt ttcgcttctt
    23401 gaatcaaatc ctcatagctg ataatttgtt ctttaaggga actggcggtt tcaagtatca
    23461 tctgcgtgat ctcaaaagat gcaggtaatt cgctgattct ttctgcgaca ggaccaatgg
    23521 cgacagaaaa attatttttt tgtccctttt ctaattcatg gaccaacgtt tgggctacct
    23581 gataactggt ctcgatcaca ttttcttcaa gagaatcaac tattaaaaat ttgatgaatc
    23641 gagaggaaac agcagagaag atcacttgtt gattctccct aaataaaaac ttcaaataat
    23701 cactgagatg agcgtaatct tcaaagtctg tactgacttg gtttgctgct aataatacgg
    23761 tcattttttt ccctgctaaa gagcgattga aagattcagc aagtttcaat gtttcactga
    23821 tcgtaagtga gcctttgaac aaaccgttca agaaatgatt ttttttcaat tcgaaaacaa
    23881 gatcagaaga agtgtgcgtt tctttttgct gcaaatctaa agcagttact gctttttcta
    23941 ttgcttgttt caattcgcta cttttaatcg gtttaagcag ataatcatct gcctgtacct
    24001 ggattgctcc tttcatataa tcgaaatcgt cgaatccact aataaatatg agacgagtcc
    24061 aaggaagtag tttccttgct tcttttgaaa aagtcaaccc gtccatgaaa ggcatttgga
    24121 tatcagtcag aatgatgtcg ggctgtaagt cgataattga agacagtgcc atctcaccat
    24181 ctccagcttc gccgacataa gtgattggat aataagaacc aaggacagtt atttgttccc
    24241 gcaagttatt gcgaatgagg tattcgtctt cgatgataaa aactttatac atgtttgttt
    24301 cctccaattg ttttgggaga tagagggatc atcagtgtga cagttgttcc ttctcggtat
    24361 ttgctgtcaa tcgtgatagg tttttgatca tttccgtaat aaagaaggag acgcttgttt
    24421 acgttgtaaa gtccgtagcc gatactgaaa tctgaatcga tccctttggc taactcttta
    24481 cgtatttctg ccagtttttc tgcagggatg ccaatcccgt tgtctataac tttgaattgg
    24541 atcatctcat caattttgca tgtggtaata cgaataaatc ctttgtgacg gacgtacttc
    24601 gttccatggt atacagcatt ttcaatcaat ggctgcaaga tcatttttaa aatagaaaca
    24661 ggaaaaagtg tttctggtat atcgatctta tagtcaagca tatggccgta tcttattttt
    24721 aagatgacta gataatcatt gatatggcta atttcttttt caagaggaat ccagtcttgt
    24781 cctttgctta aagacgtacg aaaaaagccg gaaagtgcat gggtcatagt cagagcttct
    24841 tcaaagcagt cttgttcgat caaagaaata atggcatcca aactgttata aataaaatgg
    24901 ggagtaatct gagcttgcaa tacacgaact tcactttgtg ctaaatggta ttgtttcaaa
    24961 gcgttctccc gtaaagactg gtttagatct tccgccattt tattcatgct agcggtaagt
    25021 gtttgtaatt ccagtgtttc cggcagggtt aaacgaaagc caaggttccc tgaagaaagt
    25081 tgttctgaca tctcgatcag ctgattgatc ggttcttgga cctttctctc taaaaagcga
    25141 cgattctttt tcgcatagaa aaaaatggta ctaagaactc caagttccac tattgttaat
    25201 agaatgagcg agcggacaag tgtcgtattt tttttgcttg ctaagtcgat ctccacacga
    25261 acaaaatctt gtaagatatc tgacagcagt tgagtgacag aatctacctg attgatgatc
    25321 tcttcattgg tatccataaa atcttttgcc aagatattat tgatcagctc gttttgataa
    25381 tcttccagtg tatcgatcgt acgtagcgat acatctaacg tactaatttc tttaagtgta
    25441 tttgtattat ttttgctctc tcgaatatca ttcctaagtt cttctaggat attttcctca
    25501 ttgaactcat ctacggttac ttggccagaa atcaagtccc acatactttc caaaactttt
    25561 tgatcaacct tcaaagcaat tgcatctgct ttttggatat ttgcgatcgt ctgttgataa
    25621 atcaaaagct gttgcgaata taacagtatg ctcagtagca aagggataac agcaaataga
    25681 agcatggctt tgtttgcttg ggcaaaaatc tgttttatgc ttaaattttt tgtcatgata
    25741 cttctccagt caataatttc tggttgggat gttagctata ttctgctggg aaaagacagt
    25801 ctcggaaata aaaatatctt taggtaatgt ttttcctgtt tgaatatttc caaaataacg
    25861 agtaacggta ttggctacat accagccgac atcaggttta cactcgacta cacaattgac
    25921 gattcctgag cgtagcttct cgatcatatc ttcttgtcca tcaatagtta caatgatcaa
    25981 atcttctcca ggtttgattg atgtttcttt tatcgcagct aaagcaccgt gagtcatttc
    26041 atcactatgg ctgtataata catcgatttc ggataaatca tttgtttcta aatatcgttt
    26101 catctggtct tttcctttca tccgaataaa atcaccgaca attcggtgaa caatagtcat
    26161 cggaggttta tggtcgatgg ttccatcttt ttttagggtt ggtttacgat tgattgattc
    26221 caaaaatcct tcggagcgta gcttggtcgg tgtggagttc tctgaaccag caagttctag
    26281 gattctgatt tcctgtttct ttgaatttgt gaagtgattg ctgacaaata aacccgcccg
    26341 atttccttct gctttgaaac tggggccaat gtgggtgacg aaaagctcgg gatcattcgt
    26401 ttgaatatgt ctgtcgacaa gtatgacagg gatccctgct cgctttgctt cttttaatac
    26461 agagtcccac ccggattctt gcaaaggagt aaaaatgatc atatccactt tataggcaat
    26521 gaacgtccgt atatcttgga tttgccgttc ctgattcatg tagccatttc ggtagagaac
    26581 ttggtaatct tctttttcaa gcgcagtgat aattgattca gtatgctttt tccgccagct
    26641 actctccgtt cctgattgcg aaaaaccaac gactagtggg tcaggtgctt cctttgtatt
    26701 aggtgtacac cccatcattt ggtaagcgat tacaacaagg gctaaaaaaa ataaaacacg
    26761 tttcacttta aatcactcca tctggatttg ttagtactat cttatcagat aaaagggaaa
    26821 atcaaagtca aaaggaatca atcgaaaaaa atccaagaaa cttaaaaaaa tccaagacaa
    26881 aaaaagtaca cctaaaaaat ttaaagtaaa tcccataaaa aaatcaatgt taacgctttc
    26941 tttattagct atactcggtg tatcaattga aatgggggat aaaaaatgaa aaacaaattt
    27001 agaaagattg ttgggactat cgctattttg accgcaagca ttctattaag tgcgtgtggc
    27061 aattcaggat ctgcagatga tagtacgggg tatgtaggaa tcgctatgcc gaccaaatca
    27121 gcagaaagat ggatcgctga tgggaacaac atggtctcgg aattagaaaa actgggttat
    27181 aaaacagatc ttcaatatgg agaagataaa gtagaaaatc aagtagcaca aatagaaaat
    27241 atgattacaa aaggggtaga tacacttgtg attgcttcta tcgatggttc ggctttaact
    27301 gatgtgttag ctaaagcaaa agaagcagat atcaaggtga tcgcctatga tcgtttactg
    27361 atgaattcag aaaatgtcga ttattacgcg acttttgata attttggagt aggtgtatcg
    27421 caagcagctt atattgaaga gcacttagga ttgaaagaag gaaaaggacc attcacgatt
    27481 gaattatttg gtggctcgcc tgacgacaac aacgcactaa tcaattacaa cggagtgatg
    27541 tctgttttac aaccatatat ggacaacggt cagttggtcg tgccatctgg acagacgagt
    27601 ttcagtcaaa tcgctacgct tcgctgggat ggttctacgg cgcaagcaag gatggataat
    27661 ctgttaagcg caaattatac tgaccaaaca ttggatgctg ttttgtcgcc atatgatcca
    27721 ataagtttag gaatcatttc gtcattaaaa ggcgtggggt atgggagtga aagtaaacct
    27781 ttacctgtga ttactggaca agatgcgaca gttgctggcg ttaaatcgat cattgccggg
    27841 gaacaaacac aaacgatctt taaagacaca cgtatcttag caaaaaatac gattgaaatg
    27901 atcaaagcaa tcagtgatgg agaagaggtt ccggtcaatg atactgaaac ttatgataat
    27961 ggggtgaaaa ctgtccctac gtatttagca aatacagtat ctgtcgataa agataattat
    28021 caagcagagc tgatcgacac cgattattat aaagaatccg atctgaaaaa ttaagaggaa
    28081 ccaaacaagg aactaaagag atctaagtca gagagaggat aagaattatg gcggattgta
    28141 ttttaaaaat ggcagatatc accaaaaaat tttctggaat ccgagcgtta gattgcgtga
    28201 atctgatgat tgaacgtggg gaaatccatg cattgtgcgg agaaaacgga gcgggaaaat
    28261 caacattgat gaatgtcttg agcggcttgt atccatatgg ctcctatgac ggggaaatct
    28321 tttatgatgg aaagctgtgt caatttaaag atttgaaaga tagtgaagaa aaaggcatcg
    28381 tcatcattca tcaagaatta gcactaagtc cttatctttc aataaaagag aatatttttc
    28441 tcggaaatga acaagcagaa agaggcgtga ttgattggga actgacagaa aagaaaacat
    28501 atgaactgct gaataaagtc ggtttgtcta tcgacccgaa tcttctagta tcccagattg
    28561 gcgtagggca acagcaacta gttgaaatag ctaaagcatt atcaaaatcc gtccgtctgt
    28621 tgattttgga tgagcctacc gctgcattga atgaagaaga aagcgcgaat ttgctgagat
    28681 tgatccgaga gttgaaagag caaggtgtaa cctccatcat tatttcacat aagctgaatg
    28741 aaatcgtgga tatcgcagat cgaatcacca ttttacggga tggacagacg attgagacac
    28801 tagaaaatga aatgatcagc gaagagcata ttattcgcgg aatggtgggc agagatttag
    28861 ttgatcgtta tcctgaaaga aaaccggata ttggagaagt ttattttaaa gtagaagact
    28921 ggacggtcta tcaccctctt gatcacgagc gaatcataaa cgaccacatc aacttgcatt
    28981 tgaaaaaagg agagattgta ggaatagctg gactgatggg agcgggcaga actgaattcg
    29041 ctatgagtct atttggacat tcttatggaa gcaagatatc cggacgtata ttaaaggaag
    29101 gggcagagat atcagttaag accgttcctt cagccattgc aaacggactc gcttatgtct
    29161 cggaggaccg aaaatcttta ggactgaatc tgctgatgga tattcgggaa aacgtgacgc
    29221 tgagcagttt ggataaaata agtcaaaaag gagtattaga taaagaaaaa gaagtaactg
    29281 aggcagaaaa tttccggaaa aagatgcgta taaaaacaaa ctccatccat caaattgtca
    29341 gtagtcttag tggtgggaat caacaaaaag tcgttttatc caagtggctg atgacacaac
    29401 ccgatattct ttttttggat gagccaaccc gtgggataga tgttggggca aaatatgaga
    29461 tctacacgat tatcgaagaa atggcagcaa atggcaaagc tgtctgtctt atctcttctg
    29521 aacttcctga aatattaggg atgtgtgatc gaatctatac gatgaacgaa ggacgtatta
    29581 caggagaagt ctcgcgagaa gatgcagatc aggaagtctt gatgaaatta atgacgaaag
    29641 aagaggtggg ttaagttgga acaggcaaaa aatcttacgg ataaaaaaga gaaaaaacta
    29701 aatttgaaaa gtagagcagc tgatcttttt ggtcgctaca gcatggtgat cattttagcg
    29761 ggactactta ttgcgtttca attgatgacg gatggtattt tctggcgtcc attgaatata
    29821 actaaccttg ttctgcaaaa tagccatata ttagtactag ctgcagggat gttattagtt
    29881 gtgctcttag gtcatgtgga tttgtcggta gggtcagtga tggcgtttgt tggtgcaatt
    29941 gcgggcatga tgatggtcaa tcgccaaatg aacccgtgga tgacagttat cgcatgtctt
    30001 gcagtaggcg ggctgatcgg cgcatggcaa ggtttttggg tagcttatgc agggataccg
    30061 gcgtttatcg tgactttagc aggattgctg atgtttcgtg gactgacaca agttgttcta
    30121 ggaggccaat ctctagcacc ttttccagct gttttccaaa aaatgtctac gggttattta
    30181 ccagacttaa gcgaaggaag cagccttcac ttcttgacat tgattttagg aatgatcatc
    30241 acgatcgtgt tagttgttgg tcaatggcga acaagaaatc gtcggaaaaa tcatttattt
    30301 gaagtggaat ctccaacagc tttcttggta aaggcggtat ttacagcagt cgttgtacta
    30361 ggaataactt atatctttgc ctcttatcaa gggtatccga ttattttgat cattcttggt
    30421 ttgatcgttg cagcatatgg gtttttgaca aataaaacag tagcaggtcg acagatttat
    30481 gcgacgggcg gaaatcgaaa agcggctgaa ctatcaggga tcaaaacaaa gcgaatcacg
    30541 ttctgggtat tcgtcaacat gggagtcatg gcagctttag ctggattagt tttagctgct
    30601 cgattgaatg cggcaacacc tcaagcaggt acatcgatgg aactggatgc aatggcggca
    30661 gtatattttg gtggtgcttc gacttccggc ggcattggaa caatcatggg tgcgattgtc
    30721 ggtggcttgg tcatgggtgt attgaacaat ggcatgtcga ttttaggcgt cggggtagac
    30781 tggcagcagg caatcaaagg attgatcttg cttttagccg ttgtgctaga cttgtataat
    30841 aagaaaaaac gaacaacgta ggagaacgcc ggttattcag aaaaaagact gtgacaagac
    30901 atttgtcaca gtcttttttt cgctaaaagc gaacattcat tgtttcgttt gtatagacta
    30961 aaaataatat atgttttttt accattcgta taaattctct ttttgctctt gatttttttg
    31021 cttaattgag ttaaattaca tagggcgcta aaaaaagcct tgattaaatt aataatgttc
    31081 gtgttttgct ggatgtgaac atcaggagga taatgtaatg aaagtatttg attacgaaga
    31141 tattcaatta attcctaata aatgcatcgt aaatagccgt tctgaatgtg atacaacggt
    31201 cacattaggt aaacatacat tcaaaatgcc agtagtgcct gccaacatgc agacgattat
    31261 tgatgaaaca atcgcagaat tcttagcgga aaatggttat ttctatatca tgcatcggtt
    31321 tgatgaagca gctcgaattc cgtttatcaa aaaaatgaaa aagcgtggat tgattacctc
    31381 gatcagtgtt ggcgtcaaga aagaggaata ttcctttata gagaagttgg cagaagaatc
    31441 gctaaatcct gattatatca ctatcgacat cgcgcatggg catgctaact cagttattga
    31501 tatgattcag catattaaaa aatatttgcc tgaaactttt gtgattgccg gtaatgttgg
    31561 tacaccggaa gccgtccgtg aattggaaaa tgcgggcgca gatgcaacaa aagtcgggat
    31621 tggtccagga aaagtatgta ttacaaaaat caagacaggc tttgggactg gaggctggca
    31681 actggcagct cttagatggt gtgcgaaagc agctcgcaag ccgatcatcg ctgacggtgg
    31741 gatcagaaca catggcgata ttgcaaaatc tgtccgtttt ggtgcgacaa tggtcatgat
    31801 tggttcattg tttgccggtc acgaggaatc tcctggcgaa acaaaagtcg aaaatggaat
    31861 cgtgtataaa gaatactttg gttctgcttc agaattccaa aaaggcgaaa aacgaaatgt
    31921 tgaaggaaag aaaatctgga tccaacacaa aggatctttg aaagatacac tagtggaaat
    31981 gcaacaagac cttcaatcat ctatttctta tgctggagga agagatctgg aagccattcg
    32041 aaaagttgac tatgtcatcg tgaaaaattc gatttttaat ggagacacaa tctaaaaaat
    32101 aaataaaata ttaggatgtt gtgaaaaagt tgtcctgcat taagtaataa gaacagccaa
    32161 acaaaaatag ttctttatat gttttgtttg gctggaatta ttatcgcaga tacaagcttt
    32221 tgaacatcat tattcagaat aagggactgt gataagattt ttgtcacagt cccttatgtt
    32281 ttgcaaaaaa agtttttctt ttttctcgta aattcggtaa aatcaaaaga aaaaatagac
    32341 aatagaaaag agaagcttat gtgggaaaaa gtagagaaaa aacaaggacc gatttatcgt
    32401 caaattatgg aacagattat gagagaaatc gaaaacggaa ggatcaatcc tggaagtcaa
    32461 cttgaatcag aacgaaaact agggcaactt tttggtgtga atcgttccac cgttgtccat
    32521 gcattggaag agcttaggga gcttggggta ctaacgagca aacgtggcag cggatggttt
    32581 gttaatcaga ctgaatgggg gaaattcgct gttccaagaa tcgattggcg gcaaatgatc
    32641 tctcctcgtt atgagcagac ggactggtat gagcaaaaaa taagagaagc aaagcagatc
    32701 caaaaaacat cgtttcttga tttatacgct agcgagatgc cagatgagct tttgccgaat
    32761 tttgagtttc cttcttattc tcttgaagaa atcttagcgg aagaaaaaga aatggatctt
    32821 ttaggctata ctccgttacg taacaaaatc aaacggtatt tggaaagtaa atttgaattg
    32881 gcattttccc ccaaccagct tcttgtgact ggtggcggtc agcaagcaat ctttttgatt
    32941 ttacaaacga ttctttcagc tggagagggg atagctgtag agtcaccttc ttttttttac
    33001 cgattacctc ttttcaaagc ttcaggaacg cgtttatttg ggataccgat ggatgaagaa
    33061 gggatcgact tagaaaaact gaaagaatcg atccataaaa ataaattgaa agcagtgctt
    33121 atcaacccga attttcaaaa ccctacagga aaagtgatga gtcaaaaacg acgagaaaag
    33181 ctagtagaat tgtgtcgaca gtatcggctt ccaattattg aagacgatgt atttacggat
    33241 ctttcatttc taaaccaaga agaagtcctt cctatacgaa cgattgatcc ggataatgtg
    33301 ttatacgtcg gttcgttatc tagggtttta ggaaaaacaa cgaaaatcgg ttgggtgata
    33361 ggacctgaga cttttatctc gcaactagct caagcgcaag aaatgatgga gttttcgatg
    33421 aatatcctta cacaaatcac agtggctaat gtatttaatg aagcaattga tttgaaaatg
    33481 caagatgtaa gaaaaaagtt gaaagaaaaa agccgactga taagcgcttg gggagattca
    33541 caatcgtttt tcaagcttca ttcaatttta ggtggttact atgcctggat tacttgggat
    33601 gggaaaaaac tagaccccga actagcagaa aaagtagtag atatgggtct tggaattgca
    33661 ccaagtctac tttttgggca agatataaat ggaatcagaa tcaattacag taggctggat
    33721 gaaaaccaaa taccgttgtt ttattcaaag atgaaaattc tagaaaagtg gttaacagaa
    33781 taatttcaca aagtaaatag attaaagaat ataacagctt ggatttaaat ttcaagctgt
    33841 ttcttttttt tcggaaaaag aatacgatat aaagaaacta agaaaaaacc attatttgta
    33901 cgtccgttca cgatgtgtaa ataaaataaa tgtttgcaac aaaactgtat aacaccaacg
    33961 tttttaaata gaaacagatg gttgtaagtt tgattagaaa atagaaaaaa tgcagatgaa
    34021 atataaaacg ctttccttat tgtgatttta ttcactaatg ctgttaatat gcggattggc
    34081 ataatttcct ttcatcgtga aaatttgtgc ttttagcttg tatttctcat aaatccatga
    34141 taatttatta atgaagacgg ttaaatagtg atacagtttt attcactgtt tcaatcgcag
    34201 aatgaagaga ttttcttata gatgagagag gaatgtgtga caaatggcaa acaaaaaagc
    34261 accgatcgac ttccaagcac tgctagatga agtcaatgca gatttcccag tatatcaagc
    34321 acttgatcaa gatggaaacg tagttaacaa agatttagta cctgatttat ccgatgatga
    34381 actcgttgaa ttaatgactc atatggtatg gtctcgtgta ttagaccaac gttcaacagc
    34441 gctgaaccgc caaggacgcc taggcttttt tgcaccaact gctggacaag aggcaagtca
    34501 gctggctagt gcttacgcat ttgataaaga agatgttctt cttccaggtt atcgggatgt
    34561 acctcaacta gtaaaacatg gtttaccttt atctcaagct tttttatggt ctcgaggaca
    34621 tgctgcaggt aattcttatc cggaagaatt gaaagctcta ccaccacaaa ttatcatcgg
    34681 tgcacaatat gtacaagcag ccggtgttgc attaggtttg aagaaacgaa acaagaaaaa
    34741 tgtcgtcttc acttatactg gtgatggcgg ttcatcacaa ggtgattttt acgaagcaat
    34801 caatttcgct ggcgcttacc atgcaaatgc cgtattttat atccaaaaca acgggtatgc
    34861 gatctctaca cctcgtgaaa aacaaactgc agcaaaaaca cttgcacaaa aagctgtcgc
    34921 agctgggatt cctggtattc aagtcgatgg aatggatccg cttgcagtat atactgtatc
    34981 taaaatggca cgggaatggg cgatcagtgg aaatggacca gtcctaatcg aaacactgac
    35041 ttatcgttat ggaccacata cactatctgg tgacgatcca acccgctacc gtacaaaaga
    35101 cacagatgac gattggcaga aaaaagatcc attgatccgc ttccgtaaat atttgacgga
    35161 aaaaggactt tggtctgaag aaaaagaaga acaagtcatc gaagcaacta aagaagaaat
    35221 caaagcagct attgctgaag ccgacaaagt accaaaacaa aaggtttcag atttcttgaa
    35281 aaacatgttc gaagtatctc cacaatcaat caaagaacaa attgcaattt atgaagcgaa
    35341 ggagtcgaaa taatcatggc acaaaaaaca atgatccaag caatcacaga tgccttagca
    35401 cttgaactag aaaatgacga gaatgtcgtt gtttttggtg aagatgttgg gaaaaacgga
    35461 ggagttttcc gggcaactga aggattgcag gaaaagttcg gagaagaccg agtattcgat
    35521 actcctttag ctgaatctgg aatcgcagga ttgagtttcg gtcttgcgtt agaaggattt
    35581 cgtccagttc ctgaaatcca attctttggt tttattttcg aagcaatgga cgaagtagta
    35641 gctcaaatgg ctcgtacaag atatcgaatg agcggcacta gaaatctacc catcacaatt
    35701 cgttcgccat ttggtggcgg tgttcataca ccagaattgc attcggataa tttagaaggt
    35761 ttgatcgctc agtctcctgg tattcgagta gtgattcctt ccaaccctta tgatgcaaaa
    35821 ggactattga tttcttctat tagaagtaac gatccggttg ttttcctaga acacatgaaa
    35881 ctatatcgct cattccgaga agaagtgccg gatgaagctt acgaagttcc tttggataaa
    35941 gcagcagtta ctcgtgaggg aacagatgta tctattatca catacggagc aatggtgcgt
    36001 gaagcaatca aagcagcaga caatcttgca aaagaaaata tatctgttga aatcattgat
    36061 cttcgtacag ttgcgccatt agatgtagaa acgatcattc aatccgtgga aaaaactggt
    36121 cgcgtagttg ttgtccaaga agcacaacga caagcgggcg ttgccgcgca agtagtttct
    36181 gagatctcag aacgcgctat cctttcatta gaagccccaa tcggacgagt ttctgcacct
    36241 gatacagtct tcccatttgg acaagcagaa aatgtctggt tgccaaatgc aaaagatatc
    36301 gaagacaaag taaaagaaat cgcggaattt taagaaaaga agggacgaaa caaagtcatg
    36361 gcttatcaat ttaaattgcc ggatatcggt gaaggaatcg cagaaggtga aatcgtcaaa
    36421 tggttcgtca aaccaggaga tacgatcaat gaagatgata cattactaga agttcaaaat
    36481 gacaaatcag tagaagaaat cccgtcacct gttacaggaa cggttaaaaa tgtgattgtt
    36541 ccagaaggta ccgtagcaaa tgttggggat gtattagtag aaatcgatgc accaggacac
    36601 gaagacaatg aaggggacag tggtgtagcc gcagaatccc aaactccagc taaaccggct
    36661 gctgaaccca ctgttgatac agaatcagct ggatcgtcaa gcgaaggagt cttccaattt
    36721 aagttaccgg atatcggtga aggaatcgca gaaggtgaaa tcgtcaaatg gttcgtcaaa
    36781 ccaggagata cgatcaatga agatgataca ttactagaag ttcaaaatga caaatcagta
    36841 gaagaaattc cgtcacctgt cacagggaca gttaaaaacg tgattgttcc agaaggcact
    36901 gtagcaaatg ttggggatgt attagtagaa atcgatgctc ctggacacaa tagtgcaccg
    36961 tcaactagtg cgccatcagc tgaagcacca aaagaaaaag tggaaacatc aggttcagca
    37021 agtgtcgttg aagctgccga tccaaataaa cgtgtattgg ctatgccatc tgttcgtcaa
    37081 tttgcccgtg aaaaagatgt agatatctca caagtaactg caactggaaa aggtggcaga
    37141 gtaacaaaag aggacatcga aaacttcttg gcaggtggtc caagcagtgc accagcgaaa
    37201 tcagaagcgc cagaagcggc agcaccgaaa gaagcagctc ctgcagcaga aagcaaacca
    37261 gctgcaccag caaaaccatt caagtccaac ttaggagact tggaagaacg tgtagcaatg
    37321 acgccaacac gtaaagctat cgctaaagca atggtcaaca gtaagcatac agctccacac
    37381 gtgacattgc atgatgaagt agaagtgtct aaactatggg ataatcggaa acgatttaaa
    37441 gaagttgctg ctgcaaatgg tacgaaactt actttcttgc catatgtcgt gaaagcttta
    37501 acagcgactg ttaagaaata cccagtattg aatgcatcta tcgacgatgc aaaccaagaa
    37561 atcgtttata aacattacta caatatcggt attgcaactg atacggatca cggactatat
    37621 gtaccaaatg tgaaagatgc agaccgcaaa ggaatgtttg ctattgcaga cgaaatcaat
    37681 gagaaagcaa aattagctca tgacggcaaa ctatctgcag aagatatgcg caatggaaca
    37741 attacgatca gcaatatcgg atctgtaggt ggcggttggt ttacaccagt catcaactat
    37801 ccagaagtag ctattttagg tgtaggaacg attgcacaac aaccaatcgt aaatgctgaa
    37861 ggcgaaatcg ttgtcggtcg tgtgatgaaa ctgtcattaa gtttcgatca ccgtatcgta
    37921 gatggagcaa ctgcgcaaca agcaatgaat aatatcaaac gtcttttagc tgatccagag
    37981 ctattaatga tggaaggatg ataaacaaat ggtagttgga gattttgctg tagaattaga
    38041 tacagttgta attggtgcgg gacctggtgg ctatgtagct gccatccgcg ctgctgaaat
    38101 gggacaaaaa gtagcaatca ttgaaagaga gtatattggc ggtgtttgtt tgaatgtcgg
    38161 ctgtattcct tcaaaagcac tgatagctgc aggacaccat tatcaagagt cactagattc
    38221 tgaaatgttt ggagtaacta gtgaaaacgt caaactagat tttgcaaaaa cacaagaatg
    38281 gaaagaaaac aaagttgtcc atacgttgac ttctggtgtt ggtttcctat taaaaaaaca
    38341 taaggttgaa acaatcgaag gagaagcgtt cttcgtagat gatcacacac tacgcgtcat
    38401 tcatccagat tcagctcaaa cttattcgtt caataacgca atcatcgcaa caggttcccg
    38461 tccaatcgag attccaggat ttaaattcgg aggacgtgtg ttagattcta ctggcggatt
    38521 agcattgaaa gaagtaccta agaaatttgt gatcatcggt ggcggtgtga ttggtgccga
    38581 attgggcgga gcgtacgcaa acttaggagc agaagtaacg attcttgaag gctcaccaca
    38641 aattttacca acttacgaaa aagatctagt aaaattagta gaagatgatt tcaagaaaaa
    38701 aggcgtaaca gtcgtaacga atgcaatggc gaaagaagca gtcgataacg gtgacagcgt
    38761 gacagtgaaa tatgcagtgg acgggaaaga agaatccgtt actgctgatt atgtgatggt
    38821 cactgtaggt cgtcgtccga atacagatga tatgggctta gaacaagcgg gtgtcgaagt
    38881 aggagaacga ggattgatca cggtggataa acaaggacgt acgaacgtac ctaacatcta
    38941 tgccatcggt gatatcgttc caggggcggc attagcacac aaagccagtt atgaagcaaa
    39001 aatcgctgct gaagcaatct ctggcaaaaa agtagctgta gattataaag caatgccggc
    39061 agtagccttc actgatcctg aattggcttc tgtaggtatg acgatcaaag aagcaaaaga
    39121 tgctggtatc gaagcaaaag catataaatt cccattttca gggaacggtc gggcattatc
    39181 gcttggtaaa acagaaggat tcatccgtct agttacgacg atcgaagata acgtgcttat
    39241 tggcgcgcaa atcggtggag tcggcgcaag tgatatggtc tctgaattag ctttagcaat
    39301 cgaatcagga atgaatgctg aagatatcgc cttgacgatt catcctcatc catctcttgg
    39361 cgaaatcgtc atggatgctt cggaattagc attaggttta ccaatccata tttgatttga
    39421 ataatacagg cgaaacgcaa ctgaaaaatc agttgcgttt tgttttttag aattatcaat
    39481 aatattatta tgtaaactta aaaatatgac aacttcttgg tttattacca cttcctatgc
    39541 tacactgaag aggaatatga aataggagag ttgaagatgc aagaatatca gtatccactt
    39601 gatttagatt ggacaacaga cgaaatggtc attgtaatga agatgtggga agatctggaa
    39661 cgtgcgaatg aaaaagggat aaacaatgaa gcttttttga agacttatca gcagtttaaa
    39721 actgttgtca aatccattgg tgaggaaaaa cgattgggac gggaatttga aaaagcttcc
    39781 ggttattctt tatatcgtac agtgcagcaa gcaaaaaaaa atccaaacaa aactttgaga
    39841 atgaacgggt gagataaatg gaacagatga ttgatcatat caagtcctgg cttgaagaag
    39901 ctggtgagaa aatcaaatct tctcaacaaa cattaacaat ctcgcaaaaa tcaaatcgaa
    39961 cggatctagt aacaaatgtc gatcgaaaca tccagaattt tctaatcgaa aaaatccatt
    40021 cctttgatcc agaggcgaaa atattggcag aagaagaagg gtgtaatcaa ttatcggatc
    40081 tcgatggaag agtctttatc atcgatccta ttgatggcac cttgaatttt gtgattgaag
    40141 gggaaaattt ttgtattatg ctcgctgttt atgaatccgg cgttggaaag cttggtttca
    40201 tttatgacgt gatgagaaat gaactttttt ggggtggaaa aggaataggc gtgtataaaa
    40261 atgatgagcc attgcctaga ccagcaaata aatccttatc ggaaggctta cttggtgtga
    40321 atagttatct tttcggtcat aataaatatg gaatccgaga aattgcggaa agcagcatgg
    40381 ggacacgtat gtacggatgc gctggtcttg aactagtggc aatcttgaaa ggaaaccaca
    40441 ttgcttatat ttcaaacctt agcccttggg attatgcagc gggaaatgtc ttattggaag
    40501 agtttggtat ggtaacaagc ggtctttttg gtactccttt gacatttgaa aaaagggaat
    40561 actatttagc agggacttct tgtgcatatg cagaaatcgc agaccttttg aaaaaagaag
    40621 gctgaaaaaa aggtagaaat cattcacaaa aatgataaaa acgagcaaaa gattaatttt
    40681 ctcgaaaaaa tgaaaacatt ttactatatt tcatgaaaaa agtctgctat aattagcagt
    40741 cgagtaaaat ttgccctgtc ataaagaacc aataaaaaag gagttagtta cattgaatta
    40801 tagaaacgat atccgtaacg tcgcaattat tgcccacgtt gaccacggaa aaacaacatt
    40861 agttgatgaa ctattaaaac aatcagatac attagacgca catacgcaat tgcaagaacg
    40921 tgcgatggac tcaaatgcgc tagaaaaaga acgtggtatc acgattttag ccaaaaacac
    40981 agctgtagac tataaaggta ttcgcgtaaa catcatggat actccaggac acgcggactt
    41041 cggtggcgaa gtagaacgga tcatgaaaat ggttgacggc gttgtgttgg tcgtagatgc
    41101 gtatgaagga acaatgcctc aaacacgttt tgtattgaaa aaagcactgg aacaacatat
    41161 cacaccaatc gttgtcgtaa acaagattga caaaccttct gctcgtcctg agcatgtcgt
    41221 agatgaagtt cttgaactat tcatcgaatt aggagcagat gatgatcagt tagactttcc
    41281 tgtgatttat gcatctgcac taaatggtac gtcaagcttg tcagatgatc cagcagatca
    41341 agaaccaacc atggcaccta tttttgatac aattattgaa aaaatccctg ctcctgtcga
    41401 taacagcgac gaaccattac aattccaagt ttcattactt gactacaacg attatgtagg
    41461 tcgtatcgga atcggacgtg tatttagagg aacaatcaaa gtaggagacc aagtagcatt
    41521 aattaaatta gacggaacag ttaagaaatt ccgtgtaacg aaattatttg gtttctttgg
    41581 actaaaacgt ctagagattc aagaagcaaa agctggagac ttgattgctg tctcaggtat
    41641 ggaagatatc tttgtcggtg aaacagttac tcctgtggat catcaagatg cgttacctat
    41701 ccttcacatt gacgaaccaa cattgcaaat gactttctta gttaataatt caccatttgc
    41761 cggtcgagaa ggtaaattcg taaccgctcg taagattgaa gaacgtttga tggcagaact
    41821 tcaaacagat gtatccttac gagttgaacc aacaaactct ccagatgcat ggacagtttc
    41881 tggtcgtgga gaattgcatt tgtctatctt gattgaaaac atgcgccgtg agggatacga
    41941 attgcaagta tctcgtccag aagttattga aaaagaaatc gacggtgtaa aatgcgaacc
    42001 gtttgaacgc gtgcaaatcg atacaccaga agaatatatg ggtagtgtta tcgagtcctt
    42061 gagcttacgt aaaggtgaaa tgcaagatat ggtccataca ggaaacggac aaatccgttt
    42121 gacattctta acacctgccc gcggtttgat tggttattca actgaattcc tttcaatgac
    42181 acgtggttac ggaatcatga accatacttt tgaccaatat ctgccaatgc ttcctggtca
    42241 aattggcgga cgtcaccaag gggcgcttgt ttcgatcgat acaggaaaag caacgactta
    42301 ttctatcatg agtatcgaag aacgcggtac agtcttcgta gaaccaggta cagaagtata
    42361 tgaaggaatg attatcgggg aaaacagccg tgataatgat ttgactgtca acatcacgaa
    42421 agcaaaacaa atgacaaacg tacggtctgc tactaaagac caaacatctg tcatcaaaaa
    42481 accaaaacaa ttgacgttgg aagaatcttt ggaattcttg aatgatgatg aatattgcga
    42541 agtaacacca gaaagcattc gcttgagaaa acaaatttta gagaaaaatg ctcgtgaaaa
    42601 agcatctaaa aagaaaaaat aatctgtaaa aggatgaaga gagaaaccag cttgttataa
    42661 aaaatagctg gtttcttttt ttgtccaact cttttctgtg gagaagtcat ctttgtcaat
    42721 ggacataaat tcagaaaata cgccgaaaga cgtttaaaag acttttaaaa aacttatttc
    42781 tagagagttt ttgtttcata ataagataat gtttcacgaa aggagatcaa cttgtccttc
    42841 gaagaaaatc gaagtaaaat ggcaagagaa aggagcggga agatgaacga cttattaaaa
    42901 caatactttg gctatgatga gtttagacct ggtcaaaaag aaattatcca gaaagtcatt
    42961 gatcaggaaa atgtattagg aatcatgcct accggaagtg ggaaatcaat ctgttaccag
    43021 cttccagctt tactattaga tgggcttact gtggttgtgt cccctttaat ctctttgatg
    43081 aaggatcagg tggacgcggc gaatcaatta ggtatacccg ccacttttat caatagctcg
    43141 ttagatggtt acgaaacagc tagacgcttc caagaaatcg atagacagca gtaccgttta
    43201 ctttatatcg ctcctgaacg ttttatcatg ccggatttta tccaagcaat gaagcgatgg
    43261 aacgtccgta tgatcgctat tgacgaggca cattgtatct ctcagtgggg gcatgatttc
    43321 cgcccaagtt atttacagat ggcgaaccag ctagatcaac tgccaaatcg tcctgtaatt
    43381 gttgctttga cagctacagc gactgtgcaa gtagctgctg acataaaaag acttttaaaa
    43441 attccagaaa acaaccatat tcagactggt tttgaacgag aaaatctacg atttcaagta
    43501 attaaagatc aaaaaaaaga acaatatcta atcgaatatt taaagatcaa caaaaatcaa
    43561 tctggtatca tttatgcagc aacgagaaaa gaagtagacc gtctctatca tttgcttaaa
    43621 aaattcgatt tttccgtggg tagatatcat ggtggtctaa atgagaatga gcgaacagaa
    43681 atgcaagaag cctttttata cgatcgcttg cagctgattg tagcgacaaa tgcatttggt
    43741 atgggtataa ataaaagcaa tgtccgtttt gttatccatt atcaaattcc tggctctcta
    43801 gaagcatact atcaggaagc tggtcgggcg ggaagagatg gtctttccag tgaagccatc
    43861 ctgcttttct ctcctcaaga tatccaagtc caaaagtttt ttatccagca gtcccaaaga
    43921 gaagaagggc aaaagcaaaa agaatatgaa aaattaaaag cgatgacaga atacgtgtat
    43981 attgagtcct gtcttcagca atacatccta aactattttg gcgagacaag cagtccgtgc
    44041 aaccgttgtg gtaactgttt agatgacaga gaaatagtag aagtaacgac agaagcacag
    44101 atggttcttt cttgcttaaa acgtatgggt gagaactatg gtaaacaaat gctgatgaaa
    44161 gtcttagcag gatcgaaaga acaaaagctg caagctttag gttttggaca tttatctacc
    44221 tatggactga tgaaaaacca atcgcaaaaa gaaacgatgc agttaattga atatttgata
    44281 tcgaatggct atcttctgac catcaatggt gagtatcctg ttttgaaagt aacagaaaga
    44341 gggatacaag tattgaaagg acaagaatct gtttatcgaa aggaacccaa gaaagtccag
    44401 cagctatcgg atgaagaaac cgatacacta tttgaagtgc tgcgggaact gcgaacggat
    44461 ctagcatcag aagcaggcgt tcccccgtat gtcgtatttt cagattccac attgaaagaa
    44521 atgagccgaa tacgtccatc aagcagattg gagatgcttc agataaaagg agttggccaa
    44581 agcaagcttg ataaatatgg cgaagccttt ttatctcgga taaaaaatgc ggactctaac
    44641 gttctggata agtagaaaga acgaatacgt aaaaatgaat aaaaacaact ggttaaatct
    44701 gttttttttg ctccttatga aaatcaaata tgttataatt aaggaattag aatgggaggt
    44761 aagcacagat ggattctttg tcgaaaaaac ttgcgatcca agtattaaat gatgaagcag
    44821 aacggatcaa acgattaatc cgaaatcaaa acaacagtct ctgtatctca caatgcaaag
    44881 ccttcgaaga agtggtagat acacagatgt acggcttttc ccggcaagtc ggttttgctg
    44941 tccgtatagg agtaatcgga aagaacgagg gccagctgat gttaagtgag ctagaacgag
    45001 aattgaatca gttatatagt gaggtttacc aagaaattta tgataaaaaa gaagttggca
    45061 aggaggaata gacttgccta agaaagtgaa aaaaaggcat ctattagact atagcatctt
    45121 gattccctat ttaattttat gtgtattggg attgatcatg gtatatagtt ctacgtcgta
    45181 tctattatta gaaaacggac aaaatccgtc cgcatcagtc attaaccaga gtattttttg
    45241 ggtgcttagc ttgattgcta tcgcactact ttataaaatg aaaacagatg ttttgaaaaa
    45301 ccagcgtttg atcatggcag ccatcgcagt tttgacgatt ttgctgctga ttgttgtgtt
    45361 ttttggtaag gagatcaatg gggcgaaagg ttggctgcaa attgcaggat tctcgatcca
    45421 acctgccgaa tacctaaaaa tcatttctat ctggtacctg tctctgactt tatctaaaag
    45481 acaaaacagt gtacaaaaag attttcttgg gactgtgaaa cgtccacttg caatggttat
    45541 cggacttact gcattagtcg ctattcttcc cgattttggt aatgcggcag ttatcttttt
    45601 gattattttg gttctgcttt tagccagcgg agtcaattat gtgtatactt tgattgtagg
    45661 agtaggaggc ttttgtctaa gtacttttac gatctggctg atcaatatca caaacggaaa
    45721 gatcttaccc ggacgtttac agtatatcta taatcgattt gctatttacc aaaatccttt
    45781 ctcagacgaa ttaaataaag ggcatcaact agtcaacggc tactatgcca tgttcaacgg
    45841 aggactattt ggcaggggat taggtaacag tatccagaaa aaaggatttt tacaagaagc
    45901 acaaaccgat tttatttatg caatcgtagt ggaagaactg ggagtgatta tggggatttt
    45961 gatattagcg ttgctattct ttatgattgt gcgcattgtc cttgtaggta tccgttcaaa
    46021 agatccgttc aattcgcttt tgtgcattgg gatcggcgca atgtttttga tccaagtatt
    46081 tgtcaatctt ggaggaatta ctggcgttat tcctttgaca gggatcacgt tcccattttt
    46141 aagtcaagga ggctccagtt tgctgatgct ttcgatttgt gtcggctttg tcctcaacat
    46201 cagtgcggat gaaaaacgca agagcttagg tttataataa aaaaagatgt aggagtggtt
    46261 cgatgaaaaa agttttagtt gctaacaggg gtgaaatcgc tgtacgtgta tttcgtgctt
    46321 gtacagaatt aggaattaaa actgttggaa tctatgcaga agaagatgaa tattccgtac
    46381 accgtttcaa agcggatgaa gcatatttag taggacaagg aaaaaaaccg attgatgcgt
    46441 atctagatat tgaagggatc atttctatcg cgaaagaatg cggagctgac gctattcatc
    46501 cgggttacgg tcttttgtcg gaaaacttga attttgcgca acggtgcgaa gaagaaggca
    46561 tcatttttgt tggacccaaa ctgcatcatt tagatatttt tggagataaa atcaaagcaa
    46621 aagctgcagc tatcgaagcg gggattgctt cgattccagg cacagatgga ccaatcgctt
    46681 ctatagacga tgccttggag tttgcgaaac aatacggtta tccgattatg atcaaggcag
    46741 ctctaggcgg cggcggacgc ggcatgcgtg tagctcacga tgaaaagagt gcaagagagg
    46801 gctacgaacg tgcgaaaagc gaagcaaaag cagcctttgg tagtgatgaa gtgtatgttg
    46861 aaaaatacat cgctaatcct aagcatatcg aagtacagat tctcggggat acgcatggaa
    46921 acgtcatcca tctttttgaa agagattgtt ctgttcagcg ccgtcaccaa aaagtagtag
    46981 aagtagcgcc ttgtgtctct atgaatgaac agcaacgtca aaaaatctgc caggcagctg
    47041 tccaattgat gaaatatgtc gggtatgtca acgcaggtac agtagaattt cttgttgaag
    47101 gagatgattt ttactttatc gaagtcaacc ctcgcgtcca agtggaacat acgatcacag
    47161 aaatgattac agatattgat atcgtaacaa cgcaactgtt gattgctcaa ggtttggatc
    47221 tgcataaaga aatcggattg cctcagcaag aaggaattaa attaaatggt tcagccattc
    47281 aatgccgaat cactacagaa gacccgttga ataacttttt accagacaca gggaaaatcg
    47341 atacgtatcg ttcacctggt ggttttggcg tacgtctgga tgtcggaaat gcttacgcgg
    47401 gctatgtagt gacaccgtat tttgattcat tgctagtaaa agtatgtacg catggtgcta
    47461 cctttgaaac agccattcag aaaatggaac gttgcttgaa agaatttaga atccgtggag
    47521 tgaaaacaaa cattccattt atgctcaatg tcatcacaca tcctgaattc caatcaggaa
    47581 atgcaaaaac aaccttcatt gatagtacag caacattatt tgaatttcct cgactacgag
    47641 accgcggaaa caaaacgatg aaatatatcg gtgaaattac tgtgaatggt tttccaggta
    47701 tcgaaagtgg cgaaaaacca ttttatgaag aaccacgtat gcctaaagat ctaatcacaa
    47761 gaactgatta cgtaacggct aagaatgtat tagatgcgaa gggtgccgat gctttggtcg
    47821 aatggatcaa aggacaggaa aatttgttgt tgacggatac gacatttaga gacgcccatc
    47881 aaagtctgct agcaactcgt gttcggacaa aggatttcaa gcagattgca cgtttgactg
    47941 gagaaggtct tcctgaactc ttttcaagtg agatgtgggg gggagcaaca tttgatgtgg
    48001 cttaccgctt tttgaatgaa gatccttggc aacggttgag aaaaattcgt tctttaatgc
    48061 ctaatacgtt gctgcaaatg cttttcagag gttcaaatgc cgttgggtat tctaattatc
    48121 ctgacaatgt attggtggaa tttgtcaaag aagcagcagc gcaaggaatc gatgtatttc
    48181 gaatctttga tagtttgaac tggactccac aaatggaaaa aagtatccaa gctgtacggg
    48241 atactggtaa aatcgcagag gcagctattt gctatactgg cgatatcaac gatccaagtc
    48301 gggcaaaata caatgttcag tattataaag acatggcaaa ggaactagaa caactgggtg
    48361 cgcatatcat tgcaatcaaa gatatggctg ggctgcttaa accgcaggca gcttatcgtt
    48421 tgattagtga attgaaagaa acgacagatc tgccaatcca tctgcatact catgatacca
    48481 gcgggaatgg aatcatcact tattctgctg cttcaaaagc aggagtagac atcgttgacg
    48541 tggcaatgag tgcaatgagt gggaatacta gtcagcctag catgagcagc ttgtattatg
    48601 cactggtcaa tggtccacgg ctgccagaaa tcacgattga aaatgcacag aaattaaacc
    48661 attattggga agatgtgcgc atgtattaca aaccgtttga aaacggcttg aatgctcctg
    48721 aaacagaagt atatatgcat gaaatgccag gaggacagta ttcgaatcta cagcaacagg
    48781 caaaagctgt cggattaggt catcgctggg atgaaatcaa acaaatgtat catactgtca
    48841 acttgatgtt tggcgatatc gttaaagtaa ctccatcatc taaagtagtt ggagatatgg
    48901 cactatttat ggtccaaaat gatcttacag aagaagatat ttatgaaaaa ggcgaaacac
    48961 taagtttccc tgaatctgtt gtgactttct tccaaggaga actagggcaa cccgttggcg
    49021 gatttcctaa aaaattgcaa aagattattc ttaaaggcag acctgcattg aatgaacgtc
    49081 caggtctttt ggctgaatca gtcgacttca acgaagtaaa aaaagaattg gcagaaaaaa
    49141 ttggctatga accaaaacaa gacgaagtat taagctattt gatgtatccg caagtctttt
    49201 tggactatca aaaagcatat aatcaatttg gcgatgttac attactggac acaccaacat
    49261 ttttccaagg tatacgtttg ggtgaaacaa tcaatgtaca aatcgaacgc ggaaaaacgc
    49321 ttatcattcg tttagatgaa atcggagaac ccgatatcga agggaatcgc gtactgttct
    49381 tcaatttgaa tggacaaaga cgtgaaatca ctatcaaaga taattcgatc atcagtacgg
    49441 tccaaacccg attgaaagct gaaccgacaa accgagaaca aattggagca acgatgtctg
    49501 gttctgtttt ggatgtatta gtgaaaaaag gcgataacgt taaaaaaggt gatacgttga
    49561 tgattactga agcgatgaaa atggaaacgg ccatcgaagc acgttttgac ggagaagtcg
    49621 cacatgtata tgtgtcttca ggagacacta tttcgtctgg tgaccttttg attgaagtga
    49681 ctgagaaata ataagcgaaa ggtggggaaa catgaaaaga ctgggtttgt tttggctgat
    49741 tctgctatta gttattgtgg cattctacat ggaaccagtc ttgtttccgc caaaacttga
    49801 agtgaaggaa aactctgatc aacaaatatc aaaccaaatg acaacgtgga aataccagga
    49861 gcttaaagca actggttttt ctacttatat cggaaaaccg atcgatcagc tggaaaagga
    49921 ttttggccaa gcctatgatc gattaactag cggatttggt tttgaaacaa gatattatga
    49981 aaataaagct agtcatctca catttgaagc gaacatccaa gacagcagag tcacaactgt
    50041 taaagtactg caaacagggc ctgctgatat cgcgccgttt tatttaggga tgacgatgca
    50101 agatcttacg aaaatcacta ctatctatac taatttcact tttgagtacc atgatacaga
    50161 agttggaatc gaactaatgg aagaggatat gaattatcgt ccgctaattg cttttgataa
    50221 cgacactttt gctattttat tttttgatca aacaacaaac ggcctatttt cagttgtgta
    50281 tttagataaa gatagtcttt tgaaactatt gccatatcaa gtgtttggtg aaggccttcc
    50341 acattatcaa gcagataaat cggctgattg ggaatcaatc aatcgcgtta aagaacgaaa
    50401 aagtacgact ttattaaata tgcttagaag agaagacgaa cttcccatat acaatcaaac
    50461 acttgccttt caaaaccaca gtcagctgct actacatgat tttttgacaa ggcctgagga
    50521 aatattgtca gaggaacgta tggctgattg gcaaaaatcg ctaggcagtg ctcaaacgac
    50581 agtgaaattc tcactgacga atgatgaatt ggagaagtta atcagtaaac aaaaattaga
    50641 aaatactagt gggctattta ttcatccggc gattgacgct actttttcgt ttttatattg
    50701 gtacagcgat ccttacaacc atgaccgttt catggacccg ggaacggatt ctgtaggcat
    50761 tgctttttct aaagaaaata tgttagtctt attgcaagaa gaaaacggag aaagtagtga
    50821 tagcagtgat cgttaacgaa gagctgtttc aactggagga tcaatgcctc caagtcgctg
    50881 aaatgataaa aaagagtaaa acgatggctt cttatcttga acataaacag tcaatggaag
    50941 aatctgcaaa agtttgccag ttgaaagaag cgttcttgtc taagaaagaa gcatttgaaa
    51001 atgtagcggc atatggtgag tatgcacctg attatcgtga aaaaaaacga gctgttcgaa
    51061 aagcaaaaag acagcttgat atggatgagc atgttgcagc tttccgatat agtgaaacac
    51121 agctgcaaag tctgttggat caaattgtcc aagtacttgc cgaagaagtt tctaatgaaa
    51181 tcaaagtaga tgcaggtagc ccgttttttg aaacgaaaaa acactttgga tgcggaggta
    51241 attgtcatgc aattagacaa tagtgaattt gaaataacaa aacgacgcgg gctgatcgta
    51301 tgggtctaca gcttaaaaca attaaagaat ctgaaaaagt tcggcttgtt gcattatgtc
    51361 tcaagaagaa tgaaatatgt cgtgctttac ttaaatgaag agacattgga agaaacagaa
    51421 gaacgaatcc gtaaattaca ttttgttcga aacgtagaac gttcttaccg accggatatc
    51481 gaaatgaatt ttgccgaaaa aataggaaac aaaacagtat taaaagacga aggtttcgaa
    51541 attgaagagc taaatacaga aatccgatta gctgaacatc tttaaaaatc tcttatgagc
    51601 agtttttaaa taaaaaataa cttggataac gatcgatttt tgatcattgt ccaagtattt
    51661 ttttggtata ctagtaccag taccatttct aaggagtgag tagatgcgga taatatcagg
    51721 aaattatggc gggcgccgtt taaaaagttt agcaggggcg aacaccagac caactacaga
    51781 taaagtaaaa gaatccatat tcaatatgat tggaccatat tttgatggag agacagtatt
    51841 ggatctgttt gcaggaagcg gtggtttagc tatcgaagca gtatctagag gctgcagtca
    51901 tgcggtatgc gtagacaaaa attaccaagc gctaaaaatc atcaaagaaa acatcgaaat
    51961 tacgaaagaa ccagagaaat ttaccacaat aaaacttgat gcggacaaag caatcgaacg
    52021 gctagcagcg gagaaacaac aattcgacta cttgtttctt gatccgccat atgcaaaaca
    52081 aaaaataatc gatcaaatcc ataaaatgga agaactggga cttttttcta aaaccgcagt
    52141 tatcgtatgc gaaacggata aaacagtcga attgccagaa actgtggctg gattccatca
    52201 aataagaaaa cagatttatg gcataacggc cgtaactatc tatagaaaag agggaaatca
    52261 atgaaacgtg cattattccc aggaagtttt gatcctttta caaaaggtca cttagatacg
    52321 gtggaacgtg cggctaaatt attcgatgag gtagtaattg gtgtttttat caatacttca
    52381 aagaaaagtt tgtttccccc agaagaaaga atgacattga tcacaaaagc tgtttctcat
    52441 cttcctaatg taaaagtcat gcatcaagaa aatcaattga cggtagaaac agcaaaagag
    52501 attggtgcag atgcgttgat cagaggcata cgaagtatca aagactttga atatgaacga
    52561 gagattgctc agatgaatca tcatctatat gatgaattgg aaactgtctt cttattagca
    52621 aaaccagaat atagtcacgt gagttccagt attttaaaag aagtactgca ctttggcgga
    52681 gatgtgagca gctacttacc gccagtgatc aacgaagctt tagctggaaa gcgtgaatca
    52741 aatgaaagct aaacaatggt tgaaaacagt actgattatc atatttggaa tgttgatcat
    52801 tggatttgta gtagttccct tgccgtatta tgtagaatca ccgggagcca cgatcgatct
    52861 gaaagaacta attacggtca atgataagga agataaaggt tctgggtctt tctctcttac
    52921 ctctgttggt atcaggcaag ctacaggttt cttagcttta aaagctaaaa tgaccccttt
    52981 tgaagaattg atcagtgaag aagaattgac tggtggggcg acaaatgaag aatataatca
    53041 aatgcaacag tattatatgg attcttccca aaatgcagca atcgaacaag cgttaaaact
    53101 tgcagacaag ccttataaaa tggaattcaa aggcgtatat gtcatgagtg tagagaaaac
    53161 atcgaatttc tttggcaagc tttcagttgg agatacagta acaaaagtag acgggaaaag
    53221 ctttcaaagt acagaagcat ttatggatta tgtaaaaagc caaaaagttg gacaaacaat
    53281 cgaaatcagt tatttgcata atggtgaaga aaaaacagct tctggtgatc tgatcgagtt
    53341 accaacagat aaaaaggcag gaataggtat cacgcttact gaccataccg agatcgaatc
    53401 agatacagat gttcgtatta attctggctc tatcggtggg ccatcagcgg gattgatgtt
    53461 cactttagaa atctatgaac aagtcactgg taaaaacttg cgacatggta agcaaattgc
    53521 aggaactgga acgatcaaca gtgaaggaga agttggccga atcggaggaa tcgataaaaa
    53581 agtggcaagt gcggataaag ctggcgtaga gattttcttt gcaccagatg atacgatttc
    53641 agacgatgtt aagaaagaat atcctgaaat aaaaaccaac tacgaagaag caaaagctgc
    53701 cgctaaaaaa ttgaattcct caatgaaaat cgtaccagta aaaacagtac aagaagcgct
    53761 tgactatcta gagaaaatga aatgaaacta aataattaaa gaggggttgt gacacgagtt
    53821 ctgtcacaac ccctcttttt cgtcaaaaat cttcacgata aaaataaaaa caaaagaaaa
    53881 aattgacgta attagatgtt gaagggacga ttttgccaga gctcactgtt tcttcaattt
    53941 taaatgaaag gagtaatgaa tgatttttac tggtcattct ttgaaaaaag aacaatgata
    54001 gaaaaaatca gcaagaaaat actagcttca cccaaattga tcatttggat gtctatgata
    54061 gttcttatct tttgtatcgc aagtggtttt ttattatcaa atttgctatt ttatagagaa
    54121 acagaagaga ctgctgcaaa agaaatactt tcttcgttta gttccagtgg agaagatcca
    54181 tcagctgaag gaaacgatgt agaagaggat gctcgtccaa aggtcatgta caccgatatc
    54241 aaaggaagtg tgaaggagcc tggtatctat tccttttctt cagaagaacg ggtctatgat
    54301 gtactaaagc gtgcaggtgg attattagaa gaagcggata gcgatcgaat caacttttct
    54361 gcgaagatag aagatcagca agtcctatat atccctgctg taggtgaaga gctgccagaa
    54421 catcttgatc agttggcatc ctcggaagga aaacagccta cagcagattc cgagcctccc
    54481 aaaataaata tcaatacagc cactccatca gaacttcagc agattcctgg gatcggatca
    54541 gtcaaagcgc aagaaatcat tcgatttaga gaagaaaacg gatccttcca gaaagtggaa
    54601 gacctgcagg agatttctgg tatcggtgaa aagacagttg aaaaactaaa aaacttcgtt
    54661 acaattaaat agaatgattc aaaatcgact ggcacgagca ataaatggac gctaatggat
    54721 actaagaata atccaattct aagagcttgg caggacgagg aggtagtatt tttggatagg
    54781 aaatagcttg attgttcgca aagaaacatt aaaaaaacca atgctgaatg tctatctcca
    54841 aaataaaata tctgggattc atattatgaa tacggctgtc agcggcaata acagtcaagc
    54901 tctacgagaa cgctttgcca aagatgtact tagctataca gcagataaag tatttattct
    54961 cattgggacg aatgatctag cagagcataa acaactttct aaagaaacgt accaaaaaat
    55021 ctgcagtgga tgatagttca gctgaaaaaa gactatagtt ctgatcatgc tttctttatc
    55081 acgccaccag cagtcgatga gacaaaacaa aaatatcgga ataacaagtt gatcaaagaa
    55141 tatatctctg ttctcacaga agtagcaaaa cgccatgatt gtgaagtatc gatttttata
    55201 cggaatttct tgaacaaatg gattcgaaga atttttataa tgggctaatg gatgacggac
    55261 ttcatttcgg cagtgaaggg taggatcttt tgtcagacag gatgattcgt tatatttaaa
    55321 gaatattttg tttaagcaat gagtactttt taatcaactt gaaaaaacgg aggaatataa
    55381 aacatgttag aaaaatcttc aaaagcatgc gatacgaata aagaaaaagt tactgataag
    55441 tcacataatg aacgcattcc ttgggaccag tactttatgg ctcaggcagt actgttatct
    55501 ttacgtagta cgtgtacacg tttagaagta ggagcgacac tcgttcgaga aaaaaggatc
    55561 attgctggcg gctataatgg agcagtcagt ggagatgtcc actgcataga tgaagggtgt
    55621 tacgtcgtgg atggacactg cttgcgaacg atccatgcag agatgaatgc actcctacag
    55681 tgtgcaaaac ttggtatccc gacggaaggt tcagaaatct atgtcacaca ttttccatgt
    55741 ttggcctgta caaaggcttt gttgcaagct ggtgtgaaag ggatcaatta tctccatgac
    55801 tatcgaaatg atccttatgc ccaagcgttg atcgaacaat taggtgtatc tgtacaccaa
    55861 gttgctttag actcacagca tttttcaaaa cttcaaaatg aattaacagg aaaacatgtc
    55921 tagcgtttta gaaaggcatg ttcgaaatcg gttgattttt ccggcaatta cgtcgatgat
    55981 tatctgtttg ttgtattaca ctgacaattg gggttatcgt ttgcttcttg ccggaatttt
    56041 tttatggttt tcgctaaaag aaggacgttt tatcgcgtac ttgtgtattt tgtgtagcat
    56101 aacggtatcc atttcttata ggctacaaga gtattcattt ttagaggaaa aaacaacatc
    56161 tgcgcaaatt actgtattta cagatacgat tcgtgcaaat ggtaattttt tgacaatgga
    56221 aggaaggtta tcagagaata aacaaaagct ccagctttct tatcaagtaa acacagaagc
    56281 agaattgaaa acatggctta acttccgagg aaagggaata gaattgactg ctgaaggcga
    56341 gttcatctct ccagcaaaaa agcggaatct ttataatttt gaccaagaag cttacttcag
    56401 gaaccataga atttctggac ggttccaaat tcaaaagatt gaacagatca ccatgcaaaa
    56461 tcactggcga aactggcttc agagaaaaag aggatctttt atctcctttg tccaatctga
    56521 atttccgcaa aaaacaagtg tatatattaa ttctttatta tggggctata aagatgcagg
    56581 attcagagaa ccagaagaaa tttttaggga gagtggtttg ctgcatctgt tcagtctatc
    56641 gggtctacac atccaatttt atttagggtg gctgtattat ttatttcgac gtatcggatg
    56701 gccactacac gtcagtatta ttccgttgag tctactaact atctgttatg tttctattgc
    56761 aggaagttct atttctgttt tgcgtgctag tttgctgttt ttattgcgat tgctagtcaa
    56821 attgctgaat aagcattatt ccaccatgga cttatttgca gttgtctttt ggcttctttt
    56881 actatttgat cctcgatttc tttttcatgc gggtggacag cttagttttt tcatgagttt
    56941 tttgtttatc ctgatttctt ttgatggaga ttggttgaaa aaaacggcct ctcacgtcct
    57001 gtttactatg atcacgatgc cgttgattgt atggcatttt tatgagtggc ctattttagg
    57061 tagtcttttt acattactga ttgcaccggt attcaagttt ctttttcttc ctggtgtgtt
    57121 atttctcaca ctcttcaatc gctggctgcc ggaaacttta cttttgctga tagaagaggg
    57181 attaatctta tttgagcgaa taatcgattt ttcttcaggg tcgtctctca ttataggacg
    57241 tttgcccctt gtgctatctg ggatgtatat tttcggatta ctgatttgta tggataggtt
    57301 gaaagaacaa cctgtgaaac acttgtttta tgttggtctt ttttctttta tttttttgac
    57361 tttgccattt ttacgctttt cttcaacgat cgcttttatc gatgtcggtc aaggagacag
    57421 tatctttttg caaagtcctt ttcgaaaaga aacgattttg atcgatactg gaggtcagtt
    57481 acactttgaa agagaaaagt gggcgcaagg tatgatacgt cctcctgctg aaagaaatat
    57541 cgttccatat ctaaaaggtc aggggataag tgtaatcgat aaactcatct tgacacacga
    57601 cgatacggat catgtgggag aattacccac gttatctcag cattttacta tcaaaaaaat
    57661 ttatatcggt tggggcgctg gacgaaatga gccattacaa aggcatttat ctgaagctca
    57721 aaaaaatggt actgagatca ttgaaatcaa acatggggac aggatcagcg gttattatga
    57781 tctttatgta ttgactcctt ttgaaaaggg agagggaaga aatgaagatt cgattggtct
    57841 atggatggaa tataacaata ggcgcttttt atttttagga gatttgaatc aagcaatgga
    57901 aaaacaattg cttttgattt atcctaatct gaaagccgat gtagtcaaat taggtcatca
    57961 cggtagccgt acgtcaagta acacagattt tctttcccat attgaagcaa aacatgggat
    58021 tatatcctgc ggcgtaaata atcgttatgg tcacccgcac agcgaagtat tggagacact
    58081 agaagaaaat cagataaaaa catggcgaac ggatcaacaa ggaatgatca cctatcgctg
    58141 gcatccagtt tttcatcctc atgggctagt ggaaacaatg atagactagt aattctcatc
    58201 tgctcatggt aggataattt ttgaaagtgg tgagtaattt gagtatacaa gcagagttac
    58261 aaaaaatacg taaagaaaaa cttgcatctt gttacttgat tttaggaaca gaaaagtttc
    58321 tccaagatca agttcggaca gaaattgtca aaaaaatcca attagagggc gaagatgact
    58381 taaatttttt aagttttgac atggaaaatg caagtttaga tgaggtagta gctgaagcag
    58441 agacgttgcc gtttttcgga gatcaacggt tagtattcgt agagaatcct tattttttaa
    58501 caggtgaaaa agtaaacaat ggaatcgaac aaaatactga tttattgacg gcttatttga
    58561 aagaaccatt agaatcgact gtattagtat tctttgcgcc atatgaaaag ttggatgaac
    58621 ggaaaaaagt aacgaaacaa ttgaaaaaaa cagcaattat catcaatgtc caacagttga
    58681 atgaaaaaga agtacgtcaa tatctgatga ataccttaga gaattcctca atcaaaatgg
    58741 aacgaagtgc aattgattta ttcttgaggt tgaccgactt agatttatcc aaaatgatga
    58801 gagagttaca gaaattggtc ctttatggac aaaatcaaca gcaaatcacg gtaagagaag
    58861 tagaagaatt agttcctaaa acgttagaac ataatatttt tgacatgacc caatatattt
    58921 taaaagggaa gacggaacaa gctttgcgtt tatacgaaga tttagttatg caaggggaag
    58981 aaacaatcaa aattaatgcg attttgcttt ctcaattacg tttattttta cagacaaaat
    59041 ttcttgtaaa gattggttat cagcaggcaa atattgctga aacattgaaa atccatccat
    59101 atcgtgtaaa acttgccatg caggaagtaa aaaaattcga tgaacatttg ctggtacagt
    59161 tgtttgatca attagttgag atggattatc aaatcaaaac gggacaaatc gaaaaagaat
    59221 taagttttca actattcgtt ttgcgcacag ggcagttgtt aagaaatcga taagaagaaa
    59281 gacgagggca aagcatgcgg gaaggtcgtt tgtttttgtc ctttttttga caaataaaaa
    59341 aatcggccga agccgattct tttatttagc gatttttttg cttaaacgag atttgtcgcg
    59401 gttagctttg tttttgtgga ttagaccttt tgattcagcc atatcaatag ctttaacagc
    59461 ttctttatat aacgcatcta cgttgtcagc acctgaagct acagcatctt caaatttttt
    59521 gattgcagta cgcatagcgc ttgtttgaga tgaatttttc acattagcgt tttcgcttgt
    59581 acgaacacgt ttgattgcag attcaatgtt tggcattagt ttcacctccg ataacttaag
    59641 ttcttacgcg tgttaccgca taattcaaca ttcattatta tacctaaagg cagcgtgctt
    59701 tgcaatatga acatgacaag tttttttcct taacagtctt ttattaggat tttttttcag
    59761 taaaaacgga tagtacaagc tgaaaaaaat caaaaagacg ctattgtggg aggcaatagc
    59821 gtcaaaccaa actagatttt tatttatcta tttaaacaaa gtttgaatgg gaatgtttgg
    59881 tactaagtta ttatataaca aagagaaccg tttgcaaagt tttttttgaa aagggatacg
    59941 attccctgtt gttctttgtt tttctattaa gaaactgcag ttattttata accagtatcc
    60001 tttgatcagt aaaggctatt ctggtgtacc gattatttta atgtattcat cgatattttt
    60061 tatcaacatt ttttttatga ccaactggtt ccgacggttc catttgccac ttacttccag
    60121 cacatattta tttaggggca aacgtaagat atattttgcc aaatagcgat ctttaagcag
    60181 gccattgatc acacggtcct caagctgcaa gttgatccca gtcagcatca ttggctgttc
    60241 ttgtaaaacg tacgtatcaa ttaaaagacc ttcaaaagtt tccacaagcg tcttcctttc
    60301 ttttcaaagt aacctgaaaa caggcactaa ttcttccatt ttgatattcg ttgaagtgga
    60361 tgcgatcatt ctttcttttg tccactgtaa tctagtataa caaaaaagag cagaagagcc
    60421 aaaaagaaaa aagagcatcg aattctatag gaagtctcat tggcacggcc taaattatta
    60481 caaaacattg attaaaaaaa gcccctgcat cgcaggagca tcccctatat cgttttaata
    60541 ggtcaatcaa aaagcagtat caaacacatt ttgtatcaaa tcaggaaata atagataagc
    60601 taagcccaga atcaaaagta ccgctagaag aaaccagccg attgttccta tgaaatactt
    60661 taatttttcg aacattgtat cacctcgttg tcagtatatc acggatcagt aaattggggg
    60721 tgttaagaat ctcctaaaat agacaaaatt tgttctattt tcctaaagca aatacttgta
    60781 agatacagta agtgatacca gaagcaatga ccggtccggc ggcgattcct cttaagaaga
    60841 caacacctaa aatcgtgccg aaaacaagtg caacagtgat ttgtggatca gaagcaatca
    60901 atcctacacc cttggaagat aagacagcca ctagcccacc gcaaaaaaca gctacccagc
    60961 caatagggga tttaaaggct tctaatagat cacgtagacc gatttctcct gttgcgatag
    61021 ggattagaat agccgctgtg atgatcgtca ccccaatatt gattcctttg ccttgaagcc
    61081 aagggaaaaa ggaacgggag gctggtatga gcttcataat caaaacaaaa gctgccgcta
    61141 tcatcagact ttgattttta gctaaaattg ctacaagtaa aataattcct aaaaaaagcc
    61201 aactttccat gaaatatatt cctcactttc ttcgaaacaa tcataacatg tttttagaaa
    61261 gttacaaaag taggaattca tccttcaaga cctattcata agctgttttt ttcataaaat
    61321 aaacaaaatc ataaaaaaac actttgctta tttgacagaa gacagccgcg ctttgtatag
    61381 tggacaagac ttacattttt ttgaagaatc agaaagatca ggtaaaggtc ttagtaaacg
    61441 caagccattc agaataacca atattgtgct tccttcgtgt cctacaactc caagaggtag
    61501 atttaaaaat tgcaggaagt tagaagcaat caacaagaga ataacaagaa tcgaaaaaat
    61561 aatattttgt ttgatgatcc gccgcatttt taaagatagc cgatgactgt agacaaattt
    61621 atttaagtcg cttttcatta agacgatgtc tgcgatatcc atagcggcat ctgtcccttg
    61681 ccccatagcg acacctaaag aagcggctgc taaagcaggc gcatcattga ctccatcacc
    61741 aaccatcgca tttacttggt aaagttcatt ctcttttttg actagttgtg ttttttcttc
    61801 cggagtacag cttgcatagt aatgttcaat ccctacttgt tcagcaatgg cctttgctgt
    61861 gccagaatgg tcgccggtta tcatacttgt atggatgttt tgggatttaa agtaggaaat
    61921 tacttgttgg gtatttgctt ttggaatatc tagcagacct aaaacagcaa ttaactgctg
    61981 atctttagaa agataaatca cggttttgcc ttgattttcc aaacgttcga tcatttccgc
    62041 aatctctttg ctgattatca tcgtctcagg atcatatgcg tgcttgccaa ctttccattg
    62101 cgcacctaaa taggtagttt gtaatccaaa accagtaatt tcttctattg gtaagtattg
    62161 gatttcttca ggtattgtgc aatcgaaata ttgcaaaatc gcatgagcaa gtggatgcgt
    62221 cgttttttgt tccatagcaa caagaagttg ttgtgccaaa tccttttcat caaagaaaaa
    62281 gctgtcagtt acaactggtt ttcctttagt gagcgttcct gttttatcaa aggcaatcgc
    62341 ttttaattca gaaagctttt ctaaatggat accgcctttg attaaaatcc cattttttgc
    62401 agaatttgaa agagcagcta gagaagctgg tgtagcagat gcgacaagcg cacaaggaga
    62461 agccacaact aataatacca ttcctcgata aaagctttca tcccaactcc aacctaggaa
    62521 aaagtggggg agaaggatca ttagaggaac agcaagcaaa atcaatttta cataattatt
    62581 ttcgattcgt tcgataaatg aagctgtttg agttggcgta ttctgcgctt cttctactaa
    62641 ttggataatc ttagcaaaga cagtttcgga actcgttttt gttactcgaa tcgtgatggg
    62701 atttccttga ttcagcgttc ctgcatacat ctcatcgcct aaatattttt ctacaggcac
    62761 agattcaccg cttaatgcag actcatcgat cgttgcctgt gtgctttcta aaaagccatc
    62821 tatcggaaca gctgctcctt tagcgacaaa taccaagtta tccaccttta gttgagaaac
    62881 atctacttct tgcatttgtc cattttcttt taataattgg gctttttgtg gttgaagatt
    62941 catcaaagca gtaatttctt tttgactttt attagttgtg tattcctcta atgcaccgct
    63001 taaacaaaag atgaatgtca acattgctcc ttcaaagtaa ttaccgatca aacaggcacc
    63061 aatagcagct aaagccatca ataaatccac attcagtgtc cgatcattcc acaattcttt
    63121 caagccttcc gatgtttgtt tatatccacc gatgataatg gcactgatat aaaaaagtgg
    63181 ggtgatggta agagagaatc ggtcgaagat aaaaccgaag atcataaata aaaggcagcc
    63241 gattgttgct gcaaaagctt ttttctgatt agtcatcgtt attagacctc cttgtcaaaa
    63301 caaggatagc ataataaaaa tgtaattgat aataataaca gctaaatgag aacgataatg
    63361 gttatcattt ggtgattaag cttgatgttg tttttattat ttataatgat tattattaaa
    63421 gaaaataaaa aaccagaaca tttctcaaat aagagaagtg ttctggtttt ttctacattg
    63481 caataaacca ataaccaatc aaaatgatac caaggataat acgataccag ccaaaagcgg
    63541 taaagtcgtt tttcttcaaa tagcttagta agaatttgat agctaagacg gaaaccacaa
    63601 aagatacgaa tgttcctaca agaagaacaa cagtttcttc cattttgaat gaattccctt
    63661 cagctaaata tttgacgatc ttcaagccgc ttgcgccaaa catgacagga atccctaaga
    63721 aaaatgaaaa ctctgtagcg acaaaacgcg aagcaccaat caagatagat cctaggatcg
    63781 ttgcgccaga acgggatgtt ccgggaacaa gtgccaagac ttggaataac ccaacaagaa
    63841 gagctgcttg gtacgtaaag tcattcagag aagtccaacg aggtgttttg tttttgttgc
    63901 gtttttcgac aataataaag gcaataccgt aaacgatcaa aacaatggca actggtaaga
    63961 acttgtggaa atttgcttct aaccaatcat ccaaaggcaa accaatgatg atggctggta
    64021 tgcaagcaac gatcacttta aaccaaagtg tccacgtatc ttttttttct accgttgatt
    64081 tacttggaga aaatgggttt aacttgtgaa aataaagaac gacgacagcc aagatcgccc
    64141 ctaattggat aacgacattg aacatcgaca taaattgtga actggcatcc agtttgatga
    64201 attcatctgc taggatcaaa tgaccagtac tactgatggg cagccattcc gttattcctt
    64261 caataatacc gagaatcact gctttgataa tatttgcaaa aaacataaat tccttccttc
    64321 ccaattaaga tacatcttat agtataccgt tattttttga gaaacccaat ctgtttttta
    64381 atattaagaa aaacttgaga aataaagcag ataagtacac gaagagaaaa aagaagacaa
    64441 agccctcgtt cagactttgc cttttatgat tctctggtaa aaattaaaat atccccatta
    64501 tagaaacgag tcgcgactat gtgttttccc gttatcgata taagacgtat cattgatgcc
    64561 gtggatagaa aatcgaccgt ttgcataagt gacctttgtt acgctgccat ttaaaagccc
    64621 agcacgaaca ggctgtttag gatcgatcag attaagcaaa aaggcgatcg tcaaaccatg
    64681 agatacgact aaaacatttc caccaccatt tttttcgcag tgatgggcga tatcttcaaa
    64741 tcctgtccat acacggtctc gcaatgtttc ataagtttcc gcccacccag cggtatctaa
    64801 gttgtataca gcattagcaa tttgttcaaa agaaacttct gtggtaaaca tttcatcata
    64861 tgttttaaaa ttcaatacac gagggatcac accccacatt tccatgtcat atccaccttc
    64921 aagagacccg aaacaccatt ctctaatcct tttttcaata aaataaggaa tttcttttcc
    64981 cttagaatgt tctcctagaa taatacgggc agtttcgatt gcacgaccac tgtcactaga
    65041 ataagcaaat gaaaagtcga cattttttaa cccgcgacct aaataatgaa tgccttcttg
    65101 tccttgcttt gtgaggggtg tatcacacca gccttgtact cgttcaattg tattgaacat
    65161 cgtttttccg tgtcgtacga tataaagtgt agtttctgtc attttattct cctcctaagt
    65221 cgttttaacg attgaaaaaa ggatttgtcc ttttttcctg ttcaatcgtc gttgcatcgc
    65281 catgtcctgg atagactgca aattcatcag gtaatacaaa cagctgggtc cgaatactat
    65341 gaagtaattg ttctaaattc cctgtataca gatcggttct accgatactt cctctaaaca
    65401 gggcgtcgcc agaaacgaca aaatcatcaa aaataaagct gacgcttcct atagaatgcc
    65461 ctggggtagg gacgacgcga aaagacatgt ttcctaaagt ataagttttc atttcaaatt
    65521 catattctgc aggcgagaca ataacatcag caatatcatc atgtctaccg agtccagaaa
    65581 gattcaaaat cggatcacct agccattttt gctctagtgg gctgacataa actgggattt
    65641 gataatacgc gcgtaaaggt tccactgcac cgatatgatc gtagtgagta tgggtaagca
    65701 aaatcgctac cggctgttgg tttgtttttt tgatttcttg tttgattcgt tccgcatctt
    65761 ctccagggtc aatgatcaaa agtgcttctt cgttgtagac caaataacag ttttcttcaa
    65821 tcattcctgt ttttaatctt tcgatatgca tgtgcattcc tccttgatag ccaaaacttc
    65881 tttcatccat tagtataacg cattcattgc taggaaacaa aaacaggtac ttatttctct
    65941 ttacttatct gaattatcag actatatgaa aagtatttgg atattctaaa gagaaggaaa
    66001 gtaagttttc atagcatttt aatatgtcct tgttacatta gattcatcca aaaatccaaa
    66061 ttctatatcc tatatccctg cctaatcagc agggtgtttt tttatgtttg gaaagagagc
    66121 tctttttgct aaactaggtt ttttcaggta atcttagaga gaaagaggtg gtggaaatga
    66181 tcaaacagtc aaaaatatct gtgcggcact tagtagaatt tattttgcga cgtggaagta
    66241 tcgacaatcg taaaaaaagt aaccatactg cattagaagg agcgagaatc caccgcaagc
    66301 tccaaaaaga tgcgggagaa aactatgaaa aagaagtctt tttaaaaaca acagttcaat
    66361 tagatgaata ttacctgatc gttgaaggcc gtgcagacgg tattttcaaa agagacggta
    66421 tttattatat agatgaaatc aaaacctccg aaccacgatt tgaagattta gaaccagaac
    66481 aagtggaact tttctttcat caagcaaggg tgtatgccta tatctatagt caagaacatg
    66541 atttacaaga aataaatctg caattgactt acttccagac ggcagaagaa gtgatcacga
    66601 gaaaggttga acatcagaca agagagcagt tagaaacttt ttttaaaaag ctgactgaag
    66661 attataaaga atggcttgtt tttcaagaaa actggcgcac tgtacgcaat gcctctttga
    66721 tggctttgaa attcccccat gatgagtata ggaaaggtca gcgtgaacta gcggtagctg
    66781 cttataaaac gatccgtacg aaacagaaat tgtttgtaga agcacctacc ggaacaggaa
    66841 aaacaatctc tacgttattc cccgctttga aagctgtagg ggaagaagag ggagaaaaga
    66901 tcttttatct aacggctaaa acaataacta gacaagtagc tgaagatgct atgacagcat
    66961 taaaagatac aggagctgaa gtaaaaagtg tcactttgac ggcgaaagac aagatctgct
    67021 ttttagatga gacgacctgt aatccagatc agtgcccata tgccaatggt tattacaatc
    67081 ggattaatga aggtttatgg gatcttttga atcatgagaa tcagatcact cgtgaagtca
    67141 tcgaaactta tgcaagaaag catacacttt gtcctttcga actatcatta gatgtcagta
    67201 tctggtgtga cgtaatcatt ggggattaca attatttatt tgatccaact gtttatttaa
    67261 gacgtttttt tgaagatgaa aaaaacgagg actatctgtt tttgattgac gaagcacata
    67321 atttggtgaa tcgttcccga gagatgtatt cggcagaact atcttatgaa aaaacgaaac
    67381 gaagtaagga agctatacca aaggaattca aaaaacttca tcgccgtttc aacaaactat
    67441 taaaagaatt tgattcgatc cgagaaatag caaaagaaga tcattgggat tatcatcatc
    67501 aaaaagcacc ggctgaatct ttagtaaagg caggttatca attaagcgaa aaaattaaag
    67561 aatggctggc agaatttcct gagcacccat cacaagaaca attgcttcct tattattttg
    67621 atttgcttca ttttttgaag gtcagtgaat tttatgatga tcactacgaa acaacagtcg
    67681 aaaaaacgta tcgcgatctg atagtaaaag agttttgtat tgatccaagc ttgtttcttg
    67741 agcagtcgtt ggataaagga aaaagcagta ttcttttttc tgctagtttt tcaccactct
    67801 cttattatca ggaaacactg gggggacaaa aaagtctggc ttacaaactg ccgagtcctt
    67861 tcccagaagg aaaccagcaa gttttcatcg caaattacct agagacgact tatcgaaaaa
    67921 gggaacacag tctggcaaaa ttagtagaga cgattcatgc ttttgctgat ggaaaggtcg
    67981 ggaattattt cgttttcttt ccttcttatc aatatttaga tcttgcggtg gaagcattca
    68041 gacagcaata tcccgacagc aatgtgctaa tccaagaaac ggacatgaat gaggaagaac
    68101 gcgaacgatt tttaacaaaa tttcaaacaa atcccgctga aacattgatt ggattttgcg
    68161 tacttggcgg gattttttca gaaggaatcg atttgagagg ggaccgattg atcggcagta
    68221 tgatcgtagg tgtaggtttg ccgcagatga atcatgagca ggaactgatc aaatcatatt
    68281 atgatgagaa ggaacacctt ggatttgctt acgcttatca gcttcctgga atgaacaaag
    68341 tcttgcaagc tggtggtcga gtgattcgtg gcatggcgga tcaaggaatc gttgtattgg
    68401 cagatcaacg attttctagt ttttcttatc gacgtttatt cccacaacat tggcaaacag
    68461 caagagaagt acgaagtgtt ggggaattgg aagaaggaat acagcgtttt tggttatcaa
    68521 aagaagcagt aaatcaaaaa aacgaggaat gacagacgtc agcctcgttt tttgattaac
    68581 gatagttagt gaattgcaac tcgattggta agtcaagttt acgtaataaa gcaatcacag
    68641 cttgtagatc atcacggttc ttacctgtca ctctaatctg atcttcttgg atttgtgctt
    68701 ttactttcag tttttcattt ttgatagctg tagtgatttt cttcgcgttt tcacgatcga
    68761 ttccgttgac caaatcggca gtttgtcgag ctttgccacc tagagcgtgt tctgattcgg
    68821 aaaaatggat atttttgatt ggcacatttc ttttgatcag cttaccaaat aatacatctt
    68881 tgatctgttc gattttgaaa tcatcatcgc tcaatacaac tagattgctg ttctctaatt
    68941 tgatttctac tgtagaatcc ttgaagtcaa agcgattgga caattctttt gttgtttgct
    69001 gaatcgcatt ttttacttct tctgtattga cttctgatac aatgtcgaag ctagcatctt
    69061 ttgccatagg aaccctcctg atatacatat aaatatttac catacatcta tcttatcata
    69121 ggattagggc agattcttac ctgtttgcta aaattcttct catttgagtt cgaagtattt
    69181 tattttaata gataaactgt tattcttaca tttggaatac caaagtaggg gagaggtgct
    69241 tgtgagtaaa attatgactg cgtatcataa aaatgaaaga ttaaagaaaa tagtggttat
    69301 tttgataacc ggattgaccg ctgcggtcgc tttaaattat tttttgattc cagccaatgt
    69361 cttttctgca gggatgaatg ggatcgctca aatcatttcc tcgcttttat atgaatggtt
    69421 tcatatcaag atagacacag gaacatttat ttttcttttg aatatccctg tgtttgtatt
    69481 agggtttctc aaagtaggaa aacgagcaac gatccttagt tttgtgaatg ttgtatgtgt
    69541 atcggtgatg acgatgctgc ttccagtcgg acagatcact gataatattt tgatgaatgc
    69601 cctagtaggc ggtgttttat taggtcttgg cgtaggatta tctttaaaaa tgggctttac
    69661 aactggtggg atggatatca tctctcttgt actttcacaa acaactggaa aaacagtcgg
    69721 aaactacatg ctgattctaa atggtatcat tgtagtcatc gcaggcttct tctttaactg
    69781 ggaaagtgcc ttgtatacga ttatttccat ttatgcaatg actcaagtaa ttgatgccat
    69841 ccacacgagt catcaaaaag taacagcaat gatcgttacc gtcaagccag aagaggtaac
    69901 agaagaacta gcaaaacgga tggtacgtgg aatgacatta ctgccgtctg tcggtggcta
    69961 ttcaaaaaaa caagggaaca tgatcatgat ggtcatcacc cgctacgaaa tgtatgatct
    70021 tgatcagatc gttcatgaaa tcgacgacga tgcatttatc aatattttac caactcattc
    70081 agtatttggc cggtttgcaa atgaaaacga acaaaaaatg tatcgaacaa ctggcgtatt
    70141 gccagaaata aaaaatcgtt cacaaaaaaa atgaggaatc aggtttattt tagaataaat
    70201 ttaatctatt acatctaata agaacatttt tataaattta aacgagaatc ttacttctaa
    70261 attctcattt acaagcagct aaaaaaagag tatgataaat aaaaatcgtg ttaaggagtg
    70321 tcgtacgatg agcgaagcaa accaagaacg gtctggacaa gaaatagaac taatcaaaga
    70381 acgtattctt gctgccttgg aaatggtgat tgatcctgaa ttaggaatcg acatcgtcaa
    70441 tttaggcttg atttatgata tagaattcaa tcctgaaaat ggagaaacag tcattaagat
    70501 gactctaaca actatgggct gtccattagc agatatttta acagaatcta tccatgacgc
    70561 attaaaagaa gtgccagaag taacgaaagc agaagtcaaa ctagtctggt atccagcatg
    70621 gacgacagat aagatgagcc gttatgcacg gattgcgttg gggatacgat agaaatcttg
    70681 gctgactagc tatgagaagc aaataggaaa aacaaaaaag ccttttatcc tttctagaag
    70741 aaaagagata aaaggtttct ttgtttataa aattttgtct gctatattat aagttaatta
    70801 aataaatgtg ttctattgat aaaggacatt caaatccctt tccttttgga atccttcttt
    70861 tttacatcag tttgattttc gtatagtatt ctttctcatc ttcacgataa atcgcagccc
    70921 catttttttg ttgtgcttta agactgccaa gatttttttt atttacagac aaaagtgcct
    70981 catcaatgcc gatttttttt gcctcttgta gtactaatgc taatccttgt gtggcatatc
    71041 cttttttacg ataattaggg gagatgccgt aaccaatatg gccagcgccg tttcttaatg
    71101 tttcgttcag tttatggcgc aaattaaaaa ttcctacata ctcatgttca tcactttcta
    71161 aaataaagac ggttgcggga acgtaactct caggaagatc gattcctaaa gacgtttttt
    71221 cgcatttttt tacataatct aaaaattcag caaatgtata gccataagca ttgttgataa
    71281 atccactctc atttggtcca aagctagttt gtagctgata agctttcttt aaatctgctt
    71341 cccatagttt gatcagtttc atcgtattca cctctttaag taaccttatt catttgtttt
    71401 ctactatagc actattgttg taaatagcca tgcctatttt tatttattgt gtcatatcgt
    71461 attaagaatc atcgaacaat acaaatatga attacaataa aaaaattatt tgaagttcgt
    71521 tcattcgtta cttatgatct ctagaagaaa gaaaacgtga attacttcag cgtctagaat
    71581 tccagaagga tagtaatcaa cagaagaaat aatcaatatc caaatgaaag aagatagctg
    71641 aatcgagaaa aagaggaaaa aagaaatgaa gaaaaaaata aaatagttac taatcccaga
    71701 gtcgttcgtt taacattgaa atcgatcata tggataaaac agattttttc accaaccctc
    71761 aatagatgat ggagttgctc ggatgaatta tttgtgtaga tggcataagg gagggcgtac
    71821 agaaatgaaa aagaacaaaa atatgttctt gacaaaaaac acatgtaatc ctaaactaaa
    71881 agatgtgatg cggctatggc ggaattggca gacgcgctag cttcaggcgc tagtgagggt
    71941 gacctcgtgg aagttcgagt cttcttagcc gcataatttg tttttttata aagagaaggt
    72001 acacctggag cgatgagaaa gaaaataaca tattatctaa aagaatatag gaaaaatttt
    72061 aaacaacgga accagattca aataaatcaa aaaaaatttg tgtgaaggtg ggacagttta
    72121 aaacctaata gattgattca aatagtagat ttatagattc tgatacaaga aatactggca
    72181 aacgatagtt aaggaataaa gactaaactt aaactcaaaa agaggtcatg cgaaagaata
    72241 aatcgcatga cctcttacta tattttgaat gtgtttaaac gttcaaataa tgaatcttag
    72301 taccaaccgt ttgcttccca gaatgctttc gccgcttccc aagaaccata acgtgaagta
    72361 acatattgtt ctgctactct ttcttggttt gcagcagagt agtcaccatt taagtaagaa
    72421 gaatctaatt ggtaacggcc gatgtaacgt ccgttcgttg ctgtgtaaga accgcttgat
    72481 tctttttgtg cgatccattc ttttgcagag cttgtacttg ctggagtagc agcagtttct
    72541 gttacagcag gagcaggttg tgtttcagca acaggcgctg cttgtgtttc cgtttgaacc
    72601 gtttcttgta caacaggttg ttcagcttga acaggttcag cttggactgg tgcttcttga
    72661 acggctgctt gaggttcagc agctgcttgt gcagagcctt ctgttgggat tgttaattgt
    72721 tgtcctgagt agatcaggtt aatatcagaa attgaatttg attctgcaat ggcattgatc
    72781 aaggagttat caccaacgta tttttgagaa atcgttgata gagtatcacc tgattgaacg
    72841 gtataagctt cgtccgcatg agcagtcgtt cccatgaaga atgcagcgcc ggcagccaaa
    72901 gttgttccaa aaagtaaagt tttaagtgat gtcatgaaaa atctccttta ttcgttaaaa
    72961 agttttattt gttgtgttca acgaggaata ctttaacacg tgaaaatatt tcataggtaa
    73021 aagaagcatg acaatttatg acaaatccac gttttttgta acagaatgat gttcgctcga
    73081 tgtgaaaaat gatgaggaat ttcacataat gcagaaataa tgggattaga acaggtgttc
    73141 tgatgagaaa agaaatgtat aaataacttg aaagaaattg cttttgttca tcattttatt
    73201 tacatattgc gtatattttt tgtatttttg cttgagatgt tacaataaaa gaaaaaggag
    73261 gcggttccaa tgagtaaaag ataccagaac atcatggtag cagttgacgg atcaagacaa
    73321 tcgatcaaag cgttcgctga agcccttgac ttagcaaagg acaatgaagc ccacttattt
    73381 atcgtgtcaa tcatcaacaa agttgagctg acccatagtg cctatgcctt ttcaaaaatc
    73441 tatgcagatg aaaaacaaag aacagaagta gccatgctga aaaaaatcaa tgatgccaaa
    73501 gagtacggca ttgctgagat ccacgcaata gttgaaactg gagatccaag aaatttgatt
    73561 gccaatgtca tccctggaca agaaaatatt gatttgatcg ttatgggagc gacaggaaaa
    73621 ggtgctatac aacaagcatt agttggatcg accgcttctt atgtcgtatc acatgcccct
    73681 tgcagtgtgt tggtcgtgaa gtaatgagat aagggaggaa tagaaaatgg ttcgtcctca
    73741 agaagtccgt gcgccaaaag aaaaaataga aattttagca attattgaag acggcacaca
    73801 aacaaagaag ggatactcga tcgctttagt aaagtggaaa ggaaaaaaag gtgttgctct
    73861 gagatgggat ggagataacc aacaagataa aggattccct gtcacagcta acggttatca
    73921 tccagcgtgg tttgttctgc ctgataaatt tatggaatta tacagctatg attatgcaag
    73981 agccgcagca tcaatcaaag cattagataa attaacagaa gagcagaatg aaatggagga
    74041 aaaataaaaa agacgaacca atcagccttt agaaaactga ttagttcgag gagtaaaaat
    74101 gaaaaaaggt tatttgggat gaaactagaa taatgcgtat aacttaaata aaacttaaag
    74161 caataaaatg gcaagagggt gaggcaaatg gaagcaggaa aagtattaca ggaagtaaaa
    74221 gcactatgtg cagataaaaa atatgaagaa gcgaaaagcc tgatcgaaaa gaacaaagaa
    74281 aatttggaag aaaagtaccc gatcgcacaa gaatttatag aattaaaaca aggaacgatc
    74341 cttcaccgat tcaaacattt ttttggtata acagaataga aaaaacgatt aaagatcggc
    74401 agaaatttgc tgcatcttta atcgttttct ttttaaacat gcgagggatt caattctgat
    74461 tcttatataa aaaatagatc caaataataa gaaaaattac tgaaagcgtc aattcgatga
    74521 tgtgtgttat taatacagac gttgtttccc aatgcctaaa agaaaaaatt tgataagtaa
    74581 aatatacagc aggtaaagag gccagtaaaa ctgaaaactt cttttttgtc cattttatgt
    74641 aatagaaaag aatgcttatc ataataaaaa aaggaatata aaaaacaaat tgaccgatcg
    74701 cgatcatact attgcctccg ataaataaag tccatgatac aaaataacac tttgtgcgaa
    74761 atggcgcaag cagttttgtg gatttatccg gtaaaatctg tatcgatttt gaaattagag
    74821 gagaaacttg ctataatcaa agtgaaagaa ggagtgggat tttcatggat catgtagtgt
    74881 tagaaatcga tttgcatttg atcaataata ttcgggtgat ttactatgtt gtaagtaaca
    74941 gtgtcgaaca acgtgtactg acgaataaaa taaatggtat tttagcaaag aaagacgttc
    75001 accgattcaa taatggtgaa ggaagttatt atagtatccc agtagaaaaa attatttata
    75061 cgacagtcaa agtcagagaa gatctagata ccaaaaaagc atatgaacct atttttacaa
    75121 cttattgatt gacagcagat ggaggaaaaa aatgaatcca tatgaacaac ttattcaaga
    75181 tttgatcgat ggaaaaatcg aaaagattga tgtaaaaaga gaagaattaa tggcttttcg
    75241 agaagcgtgg ctgaagttgg agaatcgtaa atatgtggtg ggaaaagcct cactgaacgg
    75301 caatgtgact tatcattacg atccgacacg tttattttag tatgaagtat tcagaaaaga
    75361 a
//
LOCUS       DACCXO010000002        64099 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.002, whole genome shotgun sequence.
ACCESSION   DACCXO010000002 DACCXO010000000
VERSION     DACCXO010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.002"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(79..1341)
                     /locus_tag="IVY70_000073"
     CDS             complement(79..1341)
                     /locus_tag="IVY70_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7957240.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(1334..2233)
                     /locus_tag="IVY70_000074"
     CDS             complement(1334..2233)
                     /locus_tag="IVY70_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7957241.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(2378..4156)
                     /gene="uvrC"
                     /locus_tag="IVY70_000075"
     CDS             complement(2378..4156)
                     /gene="uvrC"
                     /locus_tag="IVY70_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP7957242.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(4338..4652)
                     /gene="trxA"
                     /locus_tag="IVY70_000076"
     CDS             complement(4338..4652)
                     /gene="trxA"
                     /locus_tag="IVY70_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP7957243.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(4739..7099)
                     /locus_tag="IVY70_000077"
     CDS             complement(4739..7099)
                     /locus_tag="IVY70_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP7957244.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(7214..7762)
                     /locus_tag="IVY70_000078"
     CDS             complement(7214..7762)
                     /locus_tag="IVY70_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP7957245.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(7820..8257)
                     /gene="zapA"
                     /locus_tag="IVY70_000079"
     CDS             complement(7820..8257)
                     /gene="zapA"
                     /locus_tag="IVY70_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP7957246.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            8459..9376
                     /locus_tag="IVY70_000080"
     CDS             8459..9376
                     /locus_tag="IVY70_000080"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP7957247.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(9632..10543)
                     /locus_tag="IVY70_000081"
     CDS             complement(9632..10543)
                     /locus_tag="IVY70_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7957248.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(10946..11524)
                     /locus_tag="IVY70_000082"
     CDS             complement(10946..11524)
                     /locus_tag="IVY70_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7957249.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(11544..12212)
                     /locus_tag="IVY70_000083"
     CDS             complement(11544..12212)
                     /locus_tag="IVY70_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7957250.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(12227..13297)
                     /locus_tag="IVY70_000084"
     CDS             complement(12227..13297)
                     /locus_tag="IVY70_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7957251.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(13468..14832)
                     /locus_tag="IVY70_000085"
     CDS             complement(13468..14832)
                     /locus_tag="IVY70_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7957252.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLTRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(14829..16388)
                     /locus_tag="IVY70_000086"
     CDS             complement(14829..16388)
                     /locus_tag="IVY70_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7957253.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(16444..18924)
                     /locus_tag="IVY70_000087"
     CDS             complement(16444..18924)
                     /locus_tag="IVY70_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957254.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(18908..19243)
                     /locus_tag="IVY70_000088"
     CDS             complement(18908..19243)
                     /locus_tag="IVY70_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957255.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(19257..20291)
                     /locus_tag="IVY70_000089"
     CDS             complement(19257..20291)
                     /locus_tag="IVY70_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP7957256.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(20381..21061)
                     /locus_tag="IVY70_000090"
     CDS             complement(20381..21061)
                     /locus_tag="IVY70_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7957257.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(21345..22448)
                     /locus_tag="IVY70_000091"
     CDS             complement(21345..22448)
                     /locus_tag="IVY70_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP7957258.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(23077..23547)
                     /locus_tag="IVY70_000092"
     CDS             complement(23077..23547)
                     /locus_tag="IVY70_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP7957259.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            23877..25517
                     /locus_tag="IVY70_000093"
     CDS             23877..25517
                     /locus_tag="IVY70_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP7957260.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            25671..26993
                     /locus_tag="IVY70_000094"
     CDS             25671..26993
                     /locus_tag="IVY70_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP7957261.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(27061..27495)
                     /locus_tag="IVY70_000095"
     CDS             complement(27061..27495)
                     /locus_tag="IVY70_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7957262.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(27560..28396)
                     /locus_tag="IVY70_000096"
     CDS             complement(27560..28396)
                     /locus_tag="IVY70_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP7957263.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(29138..29764)
                     /locus_tag="IVY70_000097"
     CDS             complement(29138..29764)
                     /locus_tag="IVY70_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957264.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(30059..30394)
                     /locus_tag="IVY70_000098"
     CDS             complement(30059..30394)
                     /locus_tag="IVY70_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP7957265.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(30381..30665)
                     /locus_tag="IVY70_000099"
     CDS             complement(30381..30665)
                     /locus_tag="IVY70_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP7957266.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPITVQALVQQMRGAYYANH"
     gene            complement(30721..32244)
                     /locus_tag="IVY70_000100"
     CDS             complement(30721..32244)
                     /locus_tag="IVY70_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP7957267.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(32237..33412)
                     /locus_tag="IVY70_000101"
     CDS             complement(32237..33412)
                     /locus_tag="IVY70_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP7957268.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(33416..33601)
                     /locus_tag="IVY70_000102"
     CDS             complement(33416..33601)
                     /locus_tag="IVY70_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957269.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(33567..35261)
                     /locus_tag="IVY70_000103"
     CDS             complement(33567..35261)
                     /locus_tag="IVY70_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP7957270.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(35258..35731)
                     /locus_tag="IVY70_000104"
     CDS             complement(35258..35731)
                     /locus_tag="IVY70_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP7957271.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(35800..35955)
                     /locus_tag="IVY70_000105"
     CDS             complement(35800..35955)
                     /locus_tag="IVY70_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAP7957272.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(36089..36469)
                     /locus_tag="IVY70_000106"
     CDS             complement(36089..36469)
                     /locus_tag="IVY70_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP7957273.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(36473..36688)
                     /locus_tag="IVY70_000107"
     CDS             complement(36473..36688)
                     /locus_tag="IVY70_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957274.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(36691..37098)
                     /locus_tag="IVY70_000108"
     CDS             complement(36691..37098)
                     /locus_tag="IVY70_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAP7957275.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVALCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(37364..38839)
                     /locus_tag="IVY70_000109"
     CDS             complement(37364..38839)
                     /locus_tag="IVY70_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAP7957276.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(38829..39677)
                     /locus_tag="IVY70_000110"
     CDS             complement(38829..39677)
                     /locus_tag="IVY70_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAP7957277.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(39714..40076)
                     /locus_tag="IVY70_000111"
     CDS             complement(39714..40076)
                     /locus_tag="IVY70_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957278.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(40376..41000)
                     /locus_tag="IVY70_000112"
                     /pseudo
     CDS             complement(40376..41000)
                     /locus_tag="IVY70_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(40997..41308)
                     /locus_tag="IVY70_000113"
     CDS             complement(40997..41308)
                     /locus_tag="IVY70_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957279.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(41352..41645)
                     /locus_tag="IVY70_000114"
     CDS             complement(41352..41645)
                     /locus_tag="IVY70_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957280.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVKISQDECDRFMEEYQK"
     gene            41839..42486
                     /locus_tag="IVY70_000115"
     CDS             41839..42486
                     /locus_tag="IVY70_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7957281.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            42547..43692
                     /locus_tag="IVY70_000116"
     CDS             42547..43692
                     /locus_tag="IVY70_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7957282.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(43773..44078)
                     /locus_tag="IVY70_000117"
     CDS             complement(43773..44078)
                     /locus_tag="IVY70_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP7957283.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(44103..44534)
                     /gene="ruvX"
                     /locus_tag="IVY70_000118"
     CDS             complement(44103..44534)
                     /gene="ruvX"
                     /locus_tag="IVY70_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP7957284.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(44531..44797)
                     /locus_tag="IVY70_000119"
     CDS             complement(44531..44797)
                     /locus_tag="IVY70_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP7957285.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(45052..45777)
                     /locus_tag="IVY70_000120"
     CDS             complement(45052..45777)
                     /locus_tag="IVY70_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP7957286.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(46138..46208)
                     /locus_tag="IVY70_000121"
     tRNA            complement(46138..46208)
                     /locus_tag="IVY70_000121"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46174..46176),aa:Gly,seq:ccc)
     gene            46325..47077
                     /locus_tag="IVY70_000122"
     CDS             46325..47077
                     /locus_tag="IVY70_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP7957287.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            47093..47572
                     /locus_tag="IVY70_000123"
     CDS             47093..47572
                     /locus_tag="IVY70_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP7957288.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(47823..49403)
                     /locus_tag="IVY70_000124"
     CDS             complement(47823..49403)
                     /locus_tag="IVY70_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP7957289.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(49396..50139)
                     /locus_tag="IVY70_000125"
     CDS             complement(49396..50139)
                     /locus_tag="IVY70_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7957290.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(50315..51091)
                     /locus_tag="IVY70_000126"
     CDS             complement(50315..51091)
                     /locus_tag="IVY70_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7957291.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(51339..52175)
                     /locus_tag="IVY70_000127"
     CDS             complement(51339..52175)
                     /locus_tag="IVY70_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP7957292.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(52186..53115)
                     /locus_tag="IVY70_000128"
     CDS             complement(52186..53115)
                     /locus_tag="IVY70_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP7957293.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            53307..53660
                     /locus_tag="IVY70_000129"
     CDS             53307..53660
                     /locus_tag="IVY70_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP7957294.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(53734..54390)
                     /locus_tag="IVY70_000130"
     CDS             complement(53734..54390)
                     /locus_tag="IVY70_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP7957295.1"
                     /translation="MKDHIIPKATDKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            54616..56550
                     /locus_tag="IVY70_000131"
     CDS             54616..56550
                     /locus_tag="IVY70_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7957296.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(56636..57859)
                     /locus_tag="IVY70_000132"
     CDS             complement(56636..57859)
                     /locus_tag="IVY70_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP7957297.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(57853..59751)
                     /locus_tag="IVY70_000133"
     CDS             complement(57853..59751)
                     /locus_tag="IVY70_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP7957298.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(59745..60863)
                     /locus_tag="IVY70_000134"
     CDS             complement(59745..60863)
                     /locus_tag="IVY70_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP7957299.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(60946..61779)
                     /locus_tag="IVY70_000135"
     CDS             complement(60946..61779)
                     /locus_tag="IVY70_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7957300.1"
                     /translation="MTKQRIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(61772..62689)
                     /locus_tag="IVY70_000136"
     CDS             complement(61772..62689)
                     /locus_tag="IVY70_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7957301.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(62712..62891)
                     /locus_tag="IVY70_000137"
     CDS             complement(62712..62891)
                     /locus_tag="IVY70_000137"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957302.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(62916..64040)
                     /locus_tag="IVY70_000138"
     CDS             complement(62916..64040)
                     /locus_tag="IVY70_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7957303.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT        18905 a        13103 c        10751 g        21340 t
ORIGIN      
        1 aaaaaataga aataggtcta tacaaaaaag aagctgggaa caataaatgt ccagcttcct
       61 tctatatttt tctatatttt atgctttttt tcgttcattt ctcataattg aaatcgattg
      121 tttcaatgaa ctccataatc cgccttcgct atacacgagt acattcgatt tgtacatgtt
      181 tgcagacaag atcatcaata agaatgttac ggctacaagt accaacaagg aaatcaatgc
      241 tccacccata ccgactgtct catttgctaa gcgaaccggc ataatatatg aagataagaa
      301 tggaatatac gaagtgatcc gaataacgat attgtttgga tctgaagctc ctagtatcaa
      361 tccaagcata tatccgccta gagatagata agtcactggc aaaattgctt ttgctgtatc
      421 ttctgctttg ttgaccaaag agccacataa agctgctaaa acagaaaaaa tcaaaatgcc
      481 aaagatcatg aatagaagag aataccacaa gaatggtcca atcaagttat ccaatgagat
      541 accgtctaat atatttttga ccatttccat atctttgaat tgactgtatc ctataccaaa
      601 aataataccg tataatgcca tttgcgttaa tgcaacaagt aaaacacctg ttagttttcc
      661 gtaaaaatgc ttttgtgcag tcgtactgga caggatcact tccatgatcc gcgtgccttt
      721 ttcagaagcg atctcttgtg caatgatctg cgcatacgtc aaaataataa tgaacaagac
      781 aatcgttgct acataactta cgacatattg gactgtcgag ttatcttctc caactgtcat
      841 ttttcctttt gaatcaaaat tgactttttg tcgagtcaaa cttgcttgtt ggctcaagct
      901 agctacttct tctggtgaaa tgcctaattg acttgctcga agcatcgatt gcaagccagt
      961 cagttgttgt tgtatcagta gttgtgtact ttgtcctaat gaattttcgc tataaagcat
     1021 cccatttatc ttatcattct cagttgagac tactaaatac gcatcgattt tttcttccga
     1081 aagctctttt tgtgcttttt cttcagaatc aactgctttg aactcaaaat catctgtttt
     1141 gacttggctc atttgctgac tgatttgctc attatcagcc aatacgccga ttttattgac
     1201 atcactattt tgttgagcaa agtttcctgc tacataaatg atacccagca tgataaaagg
     1261 gactaatatc atgatcaaga aagaaatcga cttaacattt tttaaataga catctttggt
     1321 aataatccaa aacttacgca tcgatttcac ctgctttcaa tttgaaaatc tcttccaaag
     1381 ttggcggttg ctggttaaac ataggaatgt agccgaactg agtcgcacga gtaaagattt
     1441 ctttcccagc ttcaggatct tctaaggtaa tctctaacgt attgtcttca tgaaggatca
     1501 cttttttcac gccttcgatt tcttctactt cctgttggct taaaccagat tccaagaata
     1561 acttcgtacg gccaaatgat tcacgaatct catgtacttt tccattcaaa accatttttc
     1621 cgtttcgcaa catgatcaaa tgatcgcaga ttttttctac attatccata ttgtggctag
     1681 aaaaaatgac acatgaacca tgttttttca attccaatat tccatctttt agcaattcag
     1741 catttaccgg atctaacccg ctgaatggct catccaaaat gatcaattta ggttcatgga
     1801 ttaacgtggc gatcagctgg actttttgct gatttccttt agacaaagat tttactttat
     1861 ccgtcttttt cccttttacc tgaaattttt ccatccaaaa atcgattttt ggttcaatct
     1921 cttttcttga tttccctcta agttctgcaa aataaagcag ttggtcttga atcgttactt
     1981 tcggataaag tccccgttcc tccggaagat aaccgatgac atcataatct ttctccccaa
     2041 gatgatggcc attccataac acttctcctt ggtcttgtgt caaaaaatcc aagatcaaac
     2101 gaaaagtcgt tgtcttccca gccccgtttt ggccaattag ccccatgatt tttccatcgg
     2161 ggatttggaa agagacatga tcgacagctg tgtagttccc aaaagtttta acaagatcct
     2221 tgacttctaa catttcttca ctccttcttc aagaaatcac atatatcctt agacaccaga
     2281 cgtaagcgat ttattattta ccaacaaaac gtaaacgctc tttttgttgt acataaaatg
     2341 ataggacact ttactttctt tgtaaagtgc cctttcctca ttcctgatgt aatttatcaa
     2401 atacgttttg tgcgacattt ttcggcagtc cgatttcttg taattcttct gtcgtggctg
     2461 ctgtgatatt tttcaacgat ttgaactctt taagcagcat tttttttcgt tttggtccta
     2521 acccttcaat ttcatctaat cgggaagcaa aactgttttt actgcgtagc tgccgatgga
     2581 aagtgattgc gaatcgatgg acttcatctt ggattcgctg gagtaagaaa aactctggtg
     2641 aatttcgctc aagcggtact acagccaaat ctataccgaa tagcaactca tttgttttat
     2701 gtttatcgtt ttttgccatg ccagcaatag gaatatcgat tcctaattga ttttctaaga
     2761 catcttttgc cacatctact tgtccttttc ctccatcgat caagatcaaa tcaggtaaag
     2821 gcaacccttc gcgaatcact cgtgaatatc tgcgatagat gacttctctc attgatgcat
     2881 aatcatccgg tccgttgact gtttttattt tatatttccg atattctttt cttgccggtt
     2941 ttccatcaat gaatacgacc atcgcagcca caggattcgt ccccatgata ttagagttat
     3001 caaaagactc gatacgtatt ggggtcggga tattcatcgc gtttcctaaa cgttcaactg
     3061 ctccgatcgt ccgttcctgt tttctggcaa tcagatcaaa ccgttcattc aaagcaactg
     3121 aggcattctt attcgccaat tcaactaatt ttttcttttc tcctcgtttt ggttgcagca
     3181 ctttcgtagc taacaaagct tctacactgg gcttgtcgat cgtatcagga atcaagactt
     3241 ctttaggaat gaaatgctca ttttcctgat aaaactgacc gaggtaagtc aagaaatctt
     3301 cttctgcttc gttatagaaa ggaaacattg aaacatctcg ttcaattagc tttccttgtc
     3361 ttacgaaaaa cacttggaca cacatccatc ctttgtcaat cgcataacca aatacatctc
     3421 gatccagtaa atcagtattt gtcatttttt ggcgagtcat gattgtctca atggcacgga
     3481 tctggtctcg gtattctgca gctttttcaa actccatgtt ttcagcagct ttttccattt
     3541 tctgatggat ctctgactcg attttttcgt gtcctccgtt taaaaacttt ttcacttcat
     3601 caacgatttt cgtatagact ttcggatcta cgtcgaaata ataaggacac agacattgtc
     3661 ccaaatgata gtaaagacaa ggctccttcg tctgactagg tttgcatttt ctcagaggaa
     3721 ataatcgatc gaggatcttc tttgtctcat tcgccgcacc gacatcggga tatggtccaa
     3781 aatacagtgc tttgtctttt agtactttac gagtaatcac caaacgggga tatttttcat
     3841 tcgttatctt aaggaaagga taggttttat catcctttaa cataatgttg tatttaggat
     3901 catttttctt tatcagattg atctctaaga gcaatgcttc gatattcgat tctgtaacga
     3961 tgtattcaaa gtcttcgatt tcactaacaa gccgttccgt tttcgtatta tggctgccag
     4021 taaaatatga gcggacacga ttttttaata ttttggcttt gccgacataa atgatcgttc
     4081 catttttatc tttcatcaag taacagccgg gctgatcagg aagtaaagct aatttgtttt
     4141 tgattcgttc gttcatctgg tactcccctt catattcaat taaagttatt atagcatgtc
     4201 tgtcaaaaaa acaatccagc ctgacaacgt aattatactc aaaaaatatt aatttgaata
     4261 accttaactc aaccgcaaaa aaaccgagga cggaactgga ttcagtccca ccctcagctt
     4321 atttttgctg taagttttta taaatattga tcgatcattt gacgtagttg ttcttttgaa
     4381 tgaacaccaa cagctttttc tactacttga ccatcttttt tcaacattaa tgttggaatg
     4441 ctcatgatac cgaattgttg aggagtttct ggattttcat ccacatccat tttagcaatt
     4501 ctgaattctt cttcatcata ttcttgttcc aactgatcca aaatcggtgc ttgcatacga
     4561 catggaccac accaagttgc ccaaaaatca atcaatacaa gtcctttatc tgtttcttca
     4621 ttaaatgtct tatctgtaat tacttgtgac atatcaaaga cctccatttt taaaatctat
     4681 gattagtata gcatttctat acttggatag cgaaccatct gcttaccttt agtaagcttt
     4741 acttaaattt aacaattgtc gcaccatttc cgccttggtt tgccggtgca aactcaaagc
     4801 ttttgacgct ccgatggttt ttaagataat cagtaattcc cttgcgaagt gcgcctgttc
     4861 cttttccgtg gacaatagtc acttgaggat aaccagctag aatggcagaa tccaaatatt
     4921 gatctacctc acttagcgct tcttcgtatc gttttcctcg aaggtccaat tgattcggca
     4981 cgtgactact agattcagat cgtacagcat ggataactcg ttgtgtcggc tctttttgag
     5041 gagcaacagg cgtcagctcg tcttcggaca cattcatttt taatataccg atttctactt
     5101 gccactgatt cccgttttta cgtaaaagcg tccctctttg gccgtaagtc gtaaccaaca
     5161 cttcgtctcc cggtttcaac gttttttgtt ctttcgcctt cttcaatact ttatttttct
     5221 tcaaagtttc ttcttgatgt agatttgcta attgggtctt tgcatcgatc aactgatgct
     5281 ctttcacatt tccttgccca atctgctgtt gcattttacg aatatcagca atgatttttt
     5341 ctgctttttc ttccgcctct gacacgactt cgtttgcttt tttctttgct ttttccattt
     5401 ctttttcacg ttcttcaaag aaatagctgt aagcttcttt caaatcatcg tgaagttctt
     5461 gtgcctcaga aacaaaatga cgcatttcca gatattcggt ctctgccatt ttccggcgat
     5521 tctctaaatc tgtgatcatt tcgtttaaat cttggctctc atcattcatc aactgcttcg
     5581 cttcatcaat aacttctgta tcaagaccta atcggctaga gatttcaaac gcattgcttc
     5641 gtccaggaac gccaatcagc aaacgatacg ttggacttag tgtatccaca tcgaactcca
     5701 tgctggcatt aatcgtattt gcccgattgt atccataaac tttcaattca ggataatgcg
     5761 ttgttgccat cacataagca gactttttac caagatcatc taagattgcg attgctagag
     5821 cagcaccttc ttgcggatct gttcctgcac cgagctcgtc aaataatacc agactttttt
     5881 cattgacttt tgataaaata tctacagtgt tcgtcatatg agaagaaaag gtcgatagac
     5941 tttgctcaat cgactgttcg tctcctatat ccgcaaatac ttcctcaaaa ataccgattt
     6001 gactttcttc acctgcagga atcggtaatc ctgcttgccc catcagttgt aacagaccta
     6061 aagtcttcaa cgtgatcgtt ttcccacctg tgttaggtcc tgtaatgacg atggcttgat
     6121 agtcttttcc gattgtgatg tcattagaaa ctactttttc ctgatcgatc agtggatgtc
     6181 ttgcttgttt caagacgaca tgattgtctt cactgattcc cggcacgatg gcttttactt
     6241 ctttaccaaa tcgtgctttt gcattcatca gatccatttt accaatcaca taagcattgt
     6301 ggatgatttc ttgacgatgg ggaacaagtt cagcagaaag ttcacttaaa atacgttgaa
     6361 tctcatttcg ttcagcgatt tggtattgac gcaaacgatt attcaaatcc accacttgtt
     6421 ttggttcaat aaataaagtc tgtcctgaag cactttgatc gtggactaca ccgccaaata
     6481 caccgcggta ttcttgtttg actgggataa cgtagcggtc atttcgcatg gtgatgatcg
     6541 catcacttaa atactttgca tttttcccac ggacgatccc atctaactgt tcacgaacag
     6601 cttgttcact tcgccgaatg ttctgtctta tgctctttaa ctcaggagaa gcgtcatcag
     6661 tcacgcgtcc gtcttcatcg attgcttcct ttaaacggcg gctcaaaaca gggatcgtga
     6721 ctaactgatc tgcccacata tacaaacgtg caaattcgat ttcactgtca gaaagatcat
     6781 caatgaaacg attcaattcg cttgtcgtag acaatactct ggaaacttgt gcaagctcca
     6841 ctccattcaa gtctgctccg atctcgatcc gtttcatatg gggacggata ttttcaagtt
     6901 taggtatagg gatcccgcct cgcagacgtt gcactttcaa tccatcctca gtttcttgaa
     6961 gccagtttgt gatcgtttgt ttctcactca ctggtaccaa ttctgccagt tcttcttttc
     7021 cttgtgcagt cactacaaat tgtctgacca tctgtttgac tttttcaaat tccaatgttt
     7081 caaggattcg gttattcatt ttatcacctt atctttcact atgaaaaaag agctgtgccg
     7141 gacatacgct acgcagcctt cttaaataaa ctactctgta caaaaaacga aatattttcc
     7201 agcactattt tccttaattc aaaatattcg ttacccaaag ctcatacatc ttgttggtaa
     7261 gaaatggcgt tctctcaacg ataaaacgag cgaaactatg ctcgccgaat tgattttgga
     7321 ccacatctaa tggaacaaag gcaagtaact gcaaaatcaa aaatatagta acgtaagcca
     7381 tgattagtga caggatgcct cctgcaatcc agtctacctg tctcaagatc ggcacatacg
     7441 tcaatccatg gacaaaaacc cccacaaatc gggtaagcag ccagccaatt agcaaaataa
     7501 tcagaaaagc agttcctgca taaaaagctt catctaggtg aaaagcaaat acctgatcaa
     7561 aaaatgctaa ctttgatgtt ggggtgacag ctgggtatgg aatgtataat tccaaatgac
     7621 ttgctaatgg tttgtaaaaa tgctgggcag caataaatga caatacatat ccaatcgcga
     7681 atatgatttg caaagcaaac ccgcgacgtg ctccagaaaa aaacgcaatt agcaataaaa
     7741 ataaaatcag caaactcagc atgctacgtt cctttctctt ctaacttttt taaatttttc
     7801 ttattttgat aaaaggatct taattagaag atttttgttt aatttgtcct tttcgtttct
     7861 cgtttaaaat ctgttgtgct tccacatgat ttttaatggt atgatctgct tgaccatttt
     7921 ctttcaatac ttcccgagct tcgttctcga ttgcttcaat gcgtttcaca cgattttcaa
     7981 gctctgtaaa tttgatcatt tttttcttta attctgctgt ttcttcttcc agctccaaga
     8041 ttctttcttg tttcttcaat tgatccgaaa gcgcattgac agccatcaag attgctgctt
     8101 gttcattatc catttgtggt gataattgtt tcaattctgc taactgttca ttgatcagct
     8161 tcgttacgat atccatatga tgcttggttt cccgtccgat aatagtatat gtttggttgg
     8221 caatcactgc tttgtagcgt gttttttcgt gcgccatacg aatcatttcc tcctatttga
     8281 tgaaagtttt atttagtgta ccatgaagtt ggttctttca ccgttctcca caaatatttt
     8341 tcacatactt aactattata tgataaactt taaaaaaatg ctatgtctcg caatctctta
     8401 gaaaacgaaa agtcgtgagt tgcggtatag ttttatgtat aaaaacgagg agaattttat
     8461 gagcaacgtc attatcaaag caacaaacga ccaacttagt caaatgaaag catactatcg
     8521 ctcttatttg ttaaataaac agattcctta tactagcttt gctgcaaaaa aagacgggac
     8581 gaccattacc gcttatacat ccggaaaagt gatgtttcaa ggtacgaatg cagcaataga
     8641 agctgataaa tggggggagg cacaggataa acaagccaag ccagcaaaaa cagcctcttc
     8701 tttgccaaaa aatatcagtc aattatccgt tttaggtagt gatgaggtcg gaaatggcag
     8761 ctattttggt ccgctaacag tatgtgctgc ttatgtgaaa cgtgatcagc ttgcagaatt
     8821 acgtaaactt ggtgttagag attcaaaaga gttaaaagat agccaaatca tccagcttgc
     8881 tgacatctta aaaaaaacca tcccctataa actgcttgtc ttatctccag aaaaatacaa
     8941 tgagatccaa ccaaaataca atgccgttcg gatgaaagtc gctttgcaca atcaagcgat
     9001 ccatttgctg ctaaaaaaaa tcgagccaga aaaaccagaa gcgattttga tcgatcagtt
     9061 tacgccggaa gcaaatttta aaaaatatgc gcgtttagaa aaaaatcaat tacaagaaaa
     9121 actgtacttc gttactaaag gagaacaata tcatttagct gttgctgctg cttctatcat
     9181 cagtcgtgcc agctttttgg aagagttaca caaagcatca gctgaagctg gaatcaccct
     9241 tccatcagga gctggaacga aatctgacca agtagcggca aaactattgg agaaaggcgg
     9301 catgcctatg cttgaaaaat atgccaaact tcattttgcc aatacagaaa aagctttgaa
     9361 gctgctaaaa aaataatgga actgtgacaa aagtcatgtc acagtccctt gttccgaata
     9421 aacggtgttc tcatgccaac tcctcggtca caagtcacaa tagtcatcaa acatgagaag
     9481 ctgttttttg aaattgctcc ttgtgacgcg gagttaaacg gcaggctcac aacctcttcc
     9541 ttaaacggtg ttcaaaagct tgcatctgcg ataataagcc cggccaaacg aaaaatatga
     9601 ggagctattt ttgtttggct gggcttattt attactgaaa ataagtggtc aatgcttgtg
     9661 tcaaacttgc agcaatctgc tgctgataac tgtctgttac taattccttt aaatcactat
     9721 cattattcat ataacccatc tcgattaaaa tagaaggttg aagattttct cgaagaactt
     9781 gatagttccc agtggagatt cctcttgaat ccagaggcag atcagtgaag gagtcagcaa
     9841 ttgtctgtgc taatgtttcc tcttgtccat agtaataata tgtcgtgatt ccagttgctt
     9901 cattcgcata ttccgtagag tctgcgtgta aactgataaa gacatctgcc tttttttctt
     9961 cactgtagtc gcatatatct ccaagctgta ctaattcatc tgtagtatgg gtcaaataaa
    10021 ccgttgctcc agcatcctct agcgcctttt ttacttttat ggctgtactt aatgttatat
    10081 ctttttcttc tgtttgttca tcattgctta atgccccagt atcatttccg ccatgcccag
    10141 gatctaatac aatcactgca tcagatagct cttccggttt tgtttgtata ttctttgtga
    10201 ctttggttcc atcttcgagg gctaaatatc cagtatagcc atcatttgtc ttgatctgat
    10261 aagcattttt tgtttcagaa agaaactccg ccagctctcc gccgtttata gttgcaagtg
    10321 gggtagttag cgagtcatct gtatatagtg tacggtcatt ttttccgatt ttgattaatg
    10381 tctcttgttc tgtatcatca aatgattcct ttgtctttgt aaccgtactg ttcgtactgc
    10441 tgaccgtttg tccaactatc tttttttgac caaaaacatc tatttgaaaa cagataataa
    10501 tgataatgat tgcaaataca aaagctccaa ttattttttt cattcttgac tctcacctcg
    10561 tagatccaga ctaagggtca aaacttaaaa tcagcttaag tacgcaaaaa aggggctgta
    10621 aaagatataa ccgtagaaaa taacattact gctaaaatga tttttttcat ttttggcata
    10681 tcctgaaatc tccttttgac tattcgtggt gattcatcta cccaataaat agtttccttc
    10741 attctacaaa caattaattt cttcgttatt aaattaaaac acttcctgga acaccgacat
    10801 aataaataaa aatataccct gataatcatg agagaataac aaatttgtat ttatgaaaaa
    10861 ctacctatat aaaaaaaaca agatataacg atcttcgtca tatcttgctt cttcacctaa
    10921 ctatttttct ccctatataa cagctttacg cttctcctaa tacaccattc aaatataaat
    10981 cgatcaattg cgccacattt agcggttctt ccacaggtct tttcgaccga gtgatgtaag
    11041 gtgctaacac taagaaaaaa tgccttgctg cgatttcttt tgaaatccca tttcttagca
    11101 cagattggtt tgctgaaaag atttcttcaa aaggttcacg ataatttttc ttccatagaa
    11161 agaaaagatg atctcttgtt tcctttgaaa gattgctttc aagatcgtga agcatcagaa
    11221 acaaatcgac cagatgtgtc tcaatcaaaa actgcgccat ttttgtcaag gttacttgaa
    11281 atcccgtttc gcttgtttga acgatttctc tcaaagcttt tccaacatct tttaagtggt
    11341 tttctaaaac tgccgtatat agtttttctt tatcaccgaa ataatgatag agattgggtt
    11401 gggtgatccc aactttttgt gcgatctctc ttgttgaagt gccgagatat cccttggaca
    11461 taaacaagtc agtcgcttct ttcaaaattt tttgctgtgt atctttgttg tatcgatgga
    11521 ccatttttcc acctgctttt tggttattct ttttctttca atacaccatc ttccatcata
    11581 aatattctat cgcatttgtc aatcagccgt gtatcgtgag taaccatgat cgttgcttta
    11641 tttttgtctt ttgattcttt tgccaagata tcgactactt ctcgtgcctt ttctgaatcc
    11701 aaactagcag ttggttcatc tgctagaata atggacggat cattgtacaa tgcacgagcg
    11761 attgcgaccc tttgccgttc tccaccagat agttcttctg gatacttgtt gatcaactta
    11821 tccacgccaa gctgctcaaa taattctttt gcttcttttt gacgatgttc atgtttgatc
    11881 ttatctacta ataataactg cttttttact gtcagaaaag gaaccagatt ggaagcttgc
    11941 aaaataaagc caatttcttt aaaacgtagt ttaacgcgtt cactttcttt ttgattactg
    12001 aattcttgat cattgataaa tacagagcca ctgctaggcg tctgcaatcc gcctgccaaa
    12061 gtcaaaaaag tacttttacc tgaaccagaa ggaccgatga ttgcaacaaa ttccccttta
    12121 tcgatactca tggttgtttc tttcaatgct ttaactactt ggtcgccatc ttggaaactt
    12181 ttttctactt ctttaaattc tattgctttc atgctcttcc tccttcttat ccaattgcct
    12241 tcaacggatc gatttttacg attgtccgaa cagaaaatag tgcgcctaac acagctacta
    12301 ccagcatcaa caagctaatg acgccaaaaa acagccaatt gctttggaat ggaaccgcat
    12361 caggtagaac aagggatgtt cccactgttc ctaatagacc aagaagaata ccaacaaatg
    12421 ataaaatgaa tgtttgtgcc actacagaac gcgcaataaa tcctcctgtg atcccttgtg
    12481 ctttcattac accaaagata ttaatctttt gcatcgtcag tacataaatg aaaatcccga
    12541 tcacaatagc cgcaataacg atcaaaaatc cgatcataaa tccaaaagtc aatacttgcg
    12601 cattatatcc aggtaattca ttgatgaatt tagaaatttt gatctgttct aaatcttcag
    12661 gtaaattact gattttacct ctgacaacta ttgcgttaat ccgtgcattt tcacttgtat
    12721 cttctttttc gaagcggatc tcttggtaag cattgatggt tgtatagaga actggtgaga
    12781 cattgaattt ggcatgatcc gtaaagccag ttagtttaaa cgtcttgtca ctaccagata
    12841 acttaactgt atctcctacg gctagaccat attcttcttt caaactaata tcgcctactg
    12901 cctcatcatc gttatcaaaa gcttttcctt caacaagatt gggcatgatg aattgatttt
    12961 tatcgatacc gaagaagcta acattgattt ttccttcttc tgtactgtct ttactagtca
    13021 cgactccagg tgtctgggca agatacgcca ctttcccacc ttcgacttct tctgctatct
    13081 tcttagtaat catggacatc cctagatttg aatttgcatc tttcgaaagc acaatcgaat
    13141 ctgcctccca cttgtcgact gctgttcgat tatcttgagc taagccataa gctaacccag
    13201 tcaaaaaaaa taccaggtag gcgatcaaaa acatcacccc tgcaactaac gcataacgta
    13261 actttgaatg cataatttca tttaatgcta aaaacattta actcctcctc ctgaaataat
    13321 cattttatac aacaatttta tcacttgata aatttttggc gaatattatg ctcaaaaaat
    13381 tcttccgcta tttcattata tgaatttttt attctcgatt gattaatatt tattatatcc
    13441 ccacacaata aaagaccgtg atatttctca cagtctttta tctactctat tgctatttgc
    13501 tctcttagct agtactttaa aagctaactc attgaggtag tgaaaacgag aggtgtgttc
    13561 agacagaaat ccaaaatcct caactgctag ctgtggaatc gtagttttgt gtttcacgat
    13621 atccgcaagc aggttcaaat ctgataataa gaattcttgg cttatcagct gtccgccaat
    13681 tatttctaat gtatccggat ggtagatcag ctttgcattt actgttttgt tttcttcatc
    13741 gtattgtgga aatagatcaa attctcctga acacgaaccg gtattttgtt ccaagaatgg
    13801 cgcttcatct tctgtcagcc ctacagttcc taaaaagtag tctataatga ctgctgctgt
    13861 tatcttttgt ctgaggtcgt aagcggttgg ctgaccactc aaagttagcg ctactgctct
    13921 tgccatatgt acagctcgag taactaaagg taaaaagaga tgccgtttat ttttgtcaaa
    13981 actgactggg acgatatctc caatcgcata gatgtccgga tcacttgtct gcagatattc
    14041 attgactatc acagtctcat cctcatacaa agccactttt tcttttaaca atgtcgtatt
    14101 aggacgagtt tgggcagaaa aaatgaccat atctgccttt attgcttgag atagaagttg
    14161 tacggatact actttctctt ctgatttttt ccaatcgatc aagaattcat ttggataaaa
    14221 attgatttgc ttagtttgca gctgattttg aaaactgttg atcatatcct tatcgaaata
    14281 tcttgttaaa acattcggca tataatcaac tagatgaata atgtatcctt tatttctcaa
    14341 agcatctgct aattccaatc caatataacc agcaccgaag atcactactt cttttgcttc
    14401 ttttaaagcg gcaagtactt gcttagattg cactagattt ttataggtgt agacattttc
    14461 agcttcaatt tgtttataca aggtgacagg agaggaacct gttgctacga ttaatttatc
    14521 ataggttatg gtctcgtttt ttccttcttt ggatgcgagg atgagctgat gtttgtctgg
    14581 atcaagttca gttactctcc attcggtgat cagttttgcc ccactttctt gaagagtccg
    14641 catgttatgg gcaacttcag ctaaactacg tatctttcct tttaaataaa ggttgattcc
    14701 atttgaaaca tagcctagtc catctttatg atcttcatcg atcagtaaaa cttctgcttt
    14761 cggttgaagt tttcttagat ttagggcagc tgtgatgcct gcatgggaag caccaatgat
    14821 cacgattttc atgttttatt catccttaaa ttcaattttt ctaaataaca ttcttttaaa
    14881 aattggttca aataggggaa atcataatta tattcgttgc tagtgaatac aaaaacttta
    14941 gcggacttct gtgacgggta ctctttcaat tggctagtca aaatgatatc atactgcttt
    15001 tctttctcgt attcgactaa atcatatttg atcgtattca gcaattgttc ctggatgatt
    15061 tctttcacga tatccgccat tagcctttca taaggaagat cataagcaat ttgcagacgt
    15121 aattctaact gcttgatcag cgtccgcaaa atagatgcat agcgagcaaa taattcaaag
    15181 cgggcatttt cctcgtctac ttcagtaaaa tcaacaatag caaatgtttg tctgaccaat
    15241 ctttcacata aaaccaggaa ttgtttatct tctacttcag ttaaccgctg ttttaatcct
    15301 tcttggccaa aaatattcat ccatcctcta aaataggcaa aatgagcgtg attttgcatc
    15361 actgtcaacg caatcttttg cgaaatctct tctggaaaaa cataaaaaga gaaatattct
    15421 tctatcgatt gtttaaacgt tcgattcaat tgagctacta cgtcgtcttt gtatttttcg
    15481 cttatcaaaa actcctggac atgcgagtta tgtgtatcta atgtaaagtt actgatcatg
    15541 aaaagataaa aaaccaacgt ttcattttga ttccaaggaa aggcatattg aaagaagtaa
    15601 cgggtcaaaa atgtatggag gtccaataaa aattgtgtcg tttgaaacaa tccaacagga
    15661 agattgctat aatctggttt ggattttttc gtacagcgca ttcgcttctt cataatggaa
    15721 agccatattt caaatttcaa tcgtccttct tctgtaatag taaaactaaa ctctttttgc
    15781 agcagatcta aaaaagtgtt tcctgataaa tcactgtatt cttttttgtt ttcaagataa
    15841 ggacgtccaa accaaaataa ttggaaataa aaataacaaa tttgactttc tggaccggtc
    15901 agccgcccgc tttttatttt cagatgaaac tcatttaaag aatcatttaa ttccttcact
    15961 ctgcgagaca aagtagccga actgatatta agttccatga caagtttctg ccgatcaaat
    16021 gagccatttt ccaacagata aagaagtatc tgatattttg tagaatagcg caagtagcaa
    16081 tgataataaa catcgactgc tgctatattt ccatgtctga ttaataaaat attcccttct
    16141 tggataacaa tttccaattg tttgatattt tccaagttag cttgtaaata aaacagatct
    16201 tctttcaagg tagcccgatc aactgataag cgggtaagta cgttattaac ggaaatctct
    16261 cctttttcct ctaataaaag ttggaaaagt ttgatttgcc gttgttcatt tttttcaagc
    16321 aaactgaaaa gttccaagtc cactcctcca agcatttctg attttaacag cagaagtttc
    16381 cttctcatcc ttgaaaaaga ggatctgtga cagttatcac agatccatac aaattctaat
    16441 cacttagctg gttggcccag ctgtcagttc ccatgtgacc atgccttgat acgttgcgcc
    16501 aggttgagca ttcgtactgc tatttacctc taagagtaaa ccatcggtat cgggaaagtc
    16561 aacgagaacc tctcctttag ctgttccatc actctcataa atcaacgtgt tttgaccttg
    16621 ctgaatcaat tgttttgatc cagaatgatt gaagtataat tttgttccgc ttaattcttg
    16681 accttgatca ttcttaaata aagaagtcaa tgtccctgtc agttgccaag gagacgtgct
    16741 tggaagccga gtatcccgca catcaaaaga aaaatcgcct tttgcttcat aataacttgt
    16801 cttcaacggt ttggggagat cgttaccaaa tgtaaaatca tctggtacat taactaatgt
    16861 taattcttgt ttggggacaa gaattgtttt agcagatgtt tggacaactg ggttaggaag
    16921 cttagcacca tctgctaatg taatattgct ggaaagtaca gctgcctttg tcagtatgct
    16981 gacggattgt acagaagtat ctacagtatt taattgatag ttgtaagtaa gatgaatggt
    17041 atctcctatt ttgctcaata gatctgctgg gacagaaact ccttgttgaa tgccggtatc
    17101 tgcgatagtt gtaccattta attttaagga accattgata taagtagtat tttcaggaag
    17161 tgagatcgtg atttttttgt cggttatcgc atccccagat tttgttaacg tgaatgtcac
    17221 attttcttga atcgtatctc ctgatagagc agacagggga tctcctgtct caggttgtct
    17281 gctaaggttt tttagctgac tatctgcact cacagatgga aaattagcca ctgttatttg
    17341 aggaagagtg gtgattccag cttgttcaga ctgccatttg tacgcaaaag acggatacat
    17401 gttagtaagt acatcgtcca ccaagcgata atcaaaataa tattgccctg aagccaatgc
    17461 aggtagtgta gaagtagctg tatttacacc cccgttattt gattggatcg tttggaaaag
    17521 ttgatctgtc catcctgtcg ctaatttttg ggttcctttc gaataaagcc tgtaatggat
    17581 cgaacccttt tttagttcag ttgctgacca attggccgtg acttggtacg atgcagaaga
    17641 aaggagcttt ccccactgac tatcggctgt taagtttgtt ggtaaaaccg ttccgtctag
    17701 cttgtactgc tccaatacct ttaaagcaac aggagaaagc aatccgcctt gcgcatcttt
    17761 cccactgtac acgatattgg ctgtctgatt caatccactt gtgcttgctg gagtatgagt
    17821 cagtattgct ccttgtgtaa cgttttttcc tgtaaaatag ttgactacgc gatcaaagcg
    17881 actaaatggc aactgatcaa aacgtccttc agtcagcccc atcgtcaatg tccgactatt
    17941 ggtagagcct ggaaggttct ggttatttcc atccacgtat tctgtggtat cagcaaatgt
    18001 attatatttt cctgttgaat cacttgttga gatgtaacta ttccacgtat ttatctctgc
    18061 acttgcagaa aatgcatatg ctcctggatt caagtttccg ccagttaatt taggataaaa
    18121 tctacctgca gctggagcta aaatcgaatt gaacatacga tcttggataa tattattcga
    18181 atcataaaaa tcaacagaaa aaacggcctt tttagacata gaaggttgta atttaccctt
    18241 ggtcaaaatt ttgtttggac tcgtatcata tttatctccg ttttctgtca tcttcgcgac
    18301 attagctgta acatcaaatt gaagcgggat atcgtccacg ttgaattcgc ctcgcgtaat
    18361 aatagcaata atatctgcca atagagtatt tgtatcccac gaccccatcc caaaacgcaa
    18421 ggtatggctt cctgctgata ataaactacc gctagtaacc ttgatggggg caccggcaat
    18481 atacaagtag gcgttgttcc agtcaattgc ttttaataca tcactgcttg atacatgttc
    18541 cggcaaattg atgatcgcca caaatgcaaa tccttgtgca gtgatattca ccaaatcaag
    18601 tacatttgtt agtttcacat ggctttccca atgaaaacgg accgtgccac ctttccaagg
    18661 atcatttgaa ccatcgttca aataagtagc agtcaattta ggcaattggt tagccccagt
    18721 tagttggaca agcttcccgc cattgactgc actacgtcca ccatttggag gtacgatatt
    18781 ttcatacgtg tatggatcat aagaaatggc aggaggcgtt gctccaacag cttctgccga
    18841 aggtgaaaag ataaatatac ttaaaacaaa aagcgaagta aaaaagaagg agcgaaaaaa
    18901 tgaaaattta ttttgtagca acatgttcat cctccttttc ctttcttctc cataacaaca
    18961 accacgcaag aagccataat tctataccaa tgatcaaata aagaaaacgg atcttttctt
    19021 cattcgtctc cgggaatacg ccatgttgat tagattcttt ataaatcggt agctgtgtct
    19081 gctgttttga tgcgccattg actgaaccac ttggatgcgg aactggactt agatcataaa
    19141 atgtaatcgt acctgaactt ttggtatctg cttggactgg ctcttgccag ctgaagaaca
    19201 gcaaacttgc aataatccct cccaataaga tcgatgtttt caacgaaatc acctcatcat
    19261 gttgtttctc cttttttctt tttcttgtgg acccacaaaa acagcaaaat caaccataac
    19321 actagaactg cagcaaccaa tcctgcataa agccatggga aagattgttc ttgtactgca
    19381 aaaacggaat tttcatttac tttttgtgct ttttcttttt tgatcgtaaa gttttgtgta
    19441 aatcgccatt cattactgtc gcctgatttt gctatgattt ctgcgcggta atcgccggct
    19501 tttgctttat caccagctaa atcaagattc aagtgaaaat tcgaatttgg tgcaactaaa
    19561 aattcattag acgtattttc aatataaagc tgattttcat aaaagatctt gctcgtaaat
    19621 atcaaattgc tgataatcct aggctgagga ttttggaaac gaagctgaac agttggcttt
    19681 gcgttgacct cgtccaaatc tcctgataaa agtttcagat ccggttctat cgtctcttga
    19741 ctttcttgta aaacgactgc gagattataa gcaatacgat ttttgatttg tgctccttct
    19801 gtagcatttt ctgtttcagc ttcttcttta ggtgaaacat gaatacccgc taatacacgt
    19861 cccttaaagc tttttgctgg gattttcact tctataggta tagttgtttc tgcatgtgct
    19921 gatacattga tttccttttt cgcaatgagc aaaacgtcac ggacatcaaa cggcaatgtc
    19981 tcatctaacg ttgggttttt ttgactataa tcgatcgttc caccatcact cgttaccgcc
    20041 ggattgacag agacctcaaa tgttcgttct tcatctgaag aattgctgat aattacttct
    20101 agtgtctgat tttgttccgg tgccactaat aaatcaaaat aaccgattgc tttatctatt
    20161 tggttctctg gaaataccgg tttgattcca aagtctcctg ctgaatcctt tgcttggctg
    20221 attgacggaa aaatgaaaaa tagacttaaa atagaaaaaa acgaaacaaa cagtcgttta
    20281 ctttgattca ttgtccacca catcctttca acaacaactg cggtaaaaaa tccttcacct
    20341 atagtatttt caccgcagtt tgttgaattt atttcatact ctatgctact gggccagcca
    20401 cgaggttcca agtcaaatca gccgtgaatg ttccagctgc tgctttcggc actttcagtg
    20461 tgatatttgc tggatcataa acagaaagcc atgttcccag accttgatct tttgctgctg
    20521 taaatattgg tgtcgctgca cttgctttac tcaacccagt tacagtttga ctagttggtc
    20581 cagtggattc gttatctgac gtacttttaa cagtggatgt tccaaaatct aattcagcgc
    20641 cttgcaaaac ttgtgaaccg tttacaaaat ctgaaaccga aactgtcacg ttccaccctt
    20701 gtgcttggcc atctgcacct acacctcgac gatctgatac ttggagataa ggtgtatcgt
    20761 tttttgaagc gtctacttga tacgtttttg ttccgctttc aatctcgtgt gtaccaaatg
    20821 gcaattcagg aactacatcc aatgttaaag aacctgtctg gccagttgct ggattttccg
    20881 gatctgctgg ctctactggt tttgaaggat cagtagggtt aagaggctca acggtatttg
    20941 ttggttcagt aaaggtaata tctccttttg aagtcgcttg accctcagca aaagctggtg
    21001 ttgccaagcc gcctagaaga agtgttgaaa gtaataatgt cccaatcatg ttctttttca
    21061 taataaatca ctcctttttc ttagtgacct aagtatagta gagacagtat gcaaaaaatc
    21121 ctcatgaatt catccgctta cttgaaatta tgaccctttt tgtataaaag agaaaaaaac
    21181 aataaaatcg tttacatgaa tattcttcct tttttattaa gtatgaaaaa atatttcaat
    21241 tttatttttt cgtttcaaat tattctatta gtgacatcct tgaactataa aaataggagt
    21301 acggcatcat ctgccgcact cctatttttt tcgtcattta tttcttaaat aaaacgattc
    21361 tcacgcaatt ttattttttt gatatcttct actgtttgtg ttccagctaa ttgcatgacc
    21421 atttccaatt ctgttttaaa gaaatcaaac acttgatgga ctcctgtact tcctcctaaa
    21481 gacaatccat aaataacggg tctgccaata gcaacaagat cggcacctga tgcgattgct
    21541 ttgaaaacat gttgtccacg tcgtacaccg ctgtcaaaca cgattggtac acgtttatct
    21601 actgcttctg ccacatattg caacgaatca aacgcagcag gccccccatc taattgacgg
    21661 ccaccatggt tagataccca aattccacca gcaccagatt ctaatgatcg atagacatct
    21721 tcttctgatt gaacaccttt cacgtagact ggcaaatcag aatgagcagc gataaattct
    21781 acatcttttg gactaagttt ttgtttggaa gatttatata cagcatccat cgtttggcct
    21841 actcctgatt gataagcctg aacgatcggc attggcagcg ggaaggtaaa cccgtttctg
    21901 cggtctgttt cacggtttcc gccaactgtt gcatccgctg tcaatacgat tgcttttgct
    21961 ccattacgct tggccacttc aagaatatcc aaattgattc cattatcttt gctcatataa
    22021 aattgaaacc attgcggtgc tttctccccg cctgcttctc ttatctcttc taaagtacaa
    22081 gaagcgtaag aacttgctgt atagatcgtc ccaaaatctg ctacaccttt tgcagaagct
    22141 ttctccgcct tcacatgagc taatccatga gcagcaactg gtgccatgat gattggtgca
    22201 gtcagcattt cttcgtcaaa atgtgtagtt gtatcgggta attctacatc tcttaataca
    22261 tgaggaataa tcaaacgatg attgaatgcc cgttcatttt cttgataagt aaataagtcg
    22321 ccagcaccac tgctgatgta accataacca ccagctggaa tcacttgtga agccgcgatt
    22381 tctaaatctt ccatattgat aaaatctact attccttcat ttgtacctgc ttgatatgtt
    22441 ttttccatct caatgccttc tctctataaa atttgaatga tgaacaatat gatagcgttt
    22501 acacttttta tattactctt cgacattgat acaggcaaat aatctgttta catttcagcc
    22561 attccagatg tcactactct ggaagaaatg attgtaataa aaatagataa aaaaccgatg
    22621 ggactttgac aaaagtagtg tcaaagtccc tttttttgaa taaacggtgt tcaaaagcca
    22681 ttttctcggt attaagtcaa atttatgcaa aaatgtggaa agccattttc acaaaatttt
    22741 tcttactact cgaaactaag cgactttttc acaacctctc gataaaacgg tgttcaatca
    22801 gctgaccctc ggtattagct caaaaaatgg caaaatatga ggaactattt tgccatttgt
    22861 ctctctaata ctcaggtcag aacgttgact tcacagtctc ttccttaaac ggtgttcaaa
    22921 aacttgcatc tgcggtaata agcccaacca aacgaaaaat aggaggagct atttttgttt
    22981 ggctgttctt attacttgat gcagggcagg tttttcacga cctctgattc acggtgtttc
    23041 tttttcatca acatttttac gaaatactgt ggaagactat tgaaataagt gtggattgtt
    23101 tctttgtgtt tcaatcgttt cttgccattc ttcatttaat tttggattac cgttttcttc
    23161 cttgaactca taaggtccta attcaaaaat cccagacagt atttgattca cgatttcata
    23221 tagttcagtg tgatcagaca catgattctt gaagatcatc tcattatcac cacgtttgaa
    23281 cctcacattc gggctctcat gatgaacctc tatgcatttt tctccttttt ttaactgacg
    23341 gacttctgaa aacatctcat aaatatcttc tccagtaaaa ccttcagcaa gcgccagact
    23401 gtccacaaga ccgcctactg tcagttcaga gattattgct tgattggaat ctaattttaa
    23461 tgtatagctt gtcgcttcta ctacgccaca tgcattgcaa ccgccttctg atagttcgct
    23521 ttttctaatc aattgaactt ctttcatttt ctcttcctcc tcttaatttt ctacttatgt
    23581 atttttatca cctgacatag gtgactccta tcaacgctat catgtgcttt tttcctcaga
    23641 aatcctattt atccattcca tctatccctt attcttctac ttttctttct ctagcatata
    23701 agagatacgc atagtattca agcaaggatt gtcgatataa aaagaacaaa tactgctatt
    23761 ttgatttctt ttggctatat tacatataaa taaaatttat catacctgct ctttttgact
    23821 atatcatcat ctgacttcta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
    23881 ttacagggtt aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
    23941 aagaacgggc aaaatatgga atcactggta tgcttccaag tactgtccaa acactggaac
    24001 aacaatctgt tcaagcttat ggtcaatact taagcaaaca aacagatttg gaaaaacgta
    24061 tcttcttaat gaatctattc aatacgaatc ggacattgtt ttataaattg atgggcgaac
    24121 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
    24181 acaacgaaat tttcttgaaa ccgcaagatg ctgcatttct ttcaatcgac gaaccagaaa
    24241 acatcaaagc aagtttgaag aacgcggcag atggacgaga cattcgtttg attgttgtga
    24301 ctgatgcaga aggaatctta ggtatgggcg actggggtgt gaacggtgtt gacatcgcta
    24361 tcggcaaatt gatggtctat actgctgctg ctggtattaa tccagcacaa gttttgcctg
    24421 tatcgatcga cgctggtact aataatgaag aattattgaa taatccctta tacttaggca
    24481 atcgtcatgc tcgtgtagaa ggcgaaactt actatgaatt catcgatcaa ttcgttcaat
    24541 cagctaccga attattccca gaattgttat tgcattggga agattttgga cgaggaaatg
    24601 ctgcagcgat cttagaaaaa tacgaagatc aaatcactac tttcaacgac gatatccaag
    24661 gtacaggaat tgttgtccta gcaggtgtat taggcggatt gaatatatcc ggtgaacaat
    24721 tgaaagatca aataattttg acatttggtg ctggtactgc tggtgtgggt atcgcaaaca
    24781 tccttttaga tgagatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
    24841 atcaagtaga taaacaaggt ttattgtttg aagatacaga aggattgact cctggtcaaa
    24901 tcccattcgc tcgcaaacgc tcagatttca ctaatgctga tgaattgaca aacttagaag
    24961 cagttgtaaa agcgatccat ccgacaatca tgattggtac ttctacccaa cctggagcat
    25021 tcactgaaga aatcgtgaaa gaaatggctg ctcatacacc acgtccgatc atcatgcctt
    25081 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttaatagaa tggacggacg
    25141 gaaaagcatt agttggaaca ggtatccctg ctgctgatgt tgaatataat ggtgtaactt
    25201 accaaatcgg tcaagcaaat aatgctttga tgtacccagg tttaggactt ggtttgattg
    25261 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
    25321 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
    25381 aattttctca aacgattgct gaaactgtcg caaaatcagt tgtcgcacaa aacttgaatc
    25441 gtgaagaaat cacggatatc aaagaagcag ttgaatctgc taaatgggtt cctgaatata
    25501 aatctttaga agattaagat cgttatagta aaagaacata cagaaacttt gtatgacgtc
    25561 taaaaagaaa aaagcgactg cggcaaaaca gcggccagcc aactcctgct cttttgtcgc
    25621 ttttctttgg cttttttcat atgatacaga ataagaaagg atctgtaaaa atggcacaaa
    25681 aaacaacaac tgcaactgca gttgaggaaa agaaaaaaga aggtttttgg tccacaaaaa
    25741 tcaccggcgt aagtttgcca ctttatttga tcatggtcct tgtcctgatc attgcaatgg
    25801 ctttagggaa attaccgacg aatatgattg gaccaatcgc agttttggtt attttcggta
    25861 atttatttca ttttatcggg acaaaaatcc ctatcgtcaa aagttatttg ggtggtggtt
    25921 ccgtttttgc gatcttcgct tctgctgcga ttgctacatt tgggatctta cctgaatatg
    25981 tagtgaaatc cacagaaaac tttgtcagca atatgggctt ttgggacttt tatattgctg
    26041 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
    26101 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtgg
    26161 gcatgcttat cggaaatggt tttgcaaatt ccgttttata catctcatta cctgcgatgg
    26221 ctggtggtgt tggagccgga gccgttcctt tatcaacgat ttatgcaaat gttttaggta
    26281 cggatgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcttag
    26341 ctatcatcgg tgcagcatta gttgctcgtg ctggacaatc aatgccaaaa ttcaacggca
    26401 atggtaaatt aatggaaaaa ggcgatcttg gcgatggaaa acctcgaact gtgaaacctt
    26461 ccattccaca gttaggtgtt ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
    26521 ttggtaacta ttttgttcct aaagtccatg cttacgcatt tatgattatt atcgttgtca
    26581 tctgtaaaat cgccaaccta ttaccagagt attatgaaga tgcagctatc atgttcaata
    26641 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttgctga
    26701 atctgaatgt attagcttct gctttgacat ggcaatttgt tgtcttatgt ctgacaagtg
    26761 ttatcgcgat ttcagttgtt tcaggatttg tcggtcgttt gtttggttta tatccaattg
    26821 aatcaactat tactgctggc ttatgcaaca actcgatggg cggaacagga aatgtagccg
    26881 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcgacttg
    26941 gtggtgccat cgttttgatc atctctggtt tcttgatgca attactttct tagtcaaaaa
    27001 aacaaaaata cctgatcatc aagtatcggc ttggtgatcg ggtatttttt tgataaagat
    27061 ttacaggatt ttcaaaaatc ttttttgcat tttatcggaa tgataaccga tgtgttcata
    27121 aaattggtgt gcttctactc tgtattctgc agaattaagc cttattgtcg ttattcctcg
    27181 ttttttcgct tcttcttcta cgccagtcat caaagccttt ccgatgcctc ttttttcagc
    27241 atatttagac acagcaagtg ccataatatt gaacattgga tcgctaaaaa tcgattcata
    27301 ggactccgct tgtacatagc cagctgcttt tttagaagta ggatcttcat aaaccaagaa
    27361 aaaatttttc tctggctcct tcagcagttt cttcatttgt ctcaccgtaa cggttaatgg
    27421 tatatcatac cctaattgat ccgcattgat ttcacgtagt gcttctgcat cttctaaacg
    27481 catttcccgt aacatccaaa tcaccagcca atttctgatt ttttaaaatg aattttttta
    27541 tcatagtata ctcttttttt catttagaac aatatgtttc agtgattttg acttgcgtta
    27601 gtctagattc tcctaatgtt tgtaagatga caaaggcatt gtaacaattg accaaaagaa
    27661 ccacgaggac aattacttga aaaataaagg tcactttttt tggagacaaa accctgctaa
    27721 atcttgctcc ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata
    27781 aatcaaagcg cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa
    27841 agaaaatcgt agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca
    27901 tcaacaaaga aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca
    27961 gaaccccccc acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat
    28021 gcttggtgta gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct
    28081 gcacatgctt ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta
    28141 ctcctccaac aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc
    28201 cgcttgatac ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt
    28261 aaaaagaaat ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca
    28321 cgcctccacc catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat
    28381 aaatcagacc aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac
    28441 tcttttcttt tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt
    28501 taaggaaatt tagtgctaaa cgaaaagagg ttgtgaaaaa gctgtcctgc atcaagtaat
    28561 aagaacagcc aaacaaaaat agctcctcat aattttcgtt tggttgaact tattaccgca
    28621 gatgcaagct tttgaacacc gtttaagaaa gaggtcgtga gcctgccgtt cagctccgag
    28681 tcacaaggaa caatttcaaa aaacagcttc tcatgtttgt tgaatattgt gacttgtgac
    28741 cgaggagttg gcaggagaac accgtttaag gaagagactg tgaagtcaac gttttgacct
    28801 gattattaga gaaacaaatg gcaaaatagt tcttcatatt tcgccgtttt tcgagcgaat
    28861 gccgaggggc agctgattga acatcgttta tttggaaaaa gggattgtga caagacgttt
    28921 gtcacaatcc ctttttttat atcttattaa ttcattcttt tgttctattc ttcttcttcc
    28981 gccatgaatg agaaacccat atcaaagaac tataaaggtt atcaaaacag cgtttatgac
    29041 taaatgaatt agagttatct atcactattt ttcaagcgat ttaaaccaaa tggcatacaa
    29101 tatggcatac aaaaacttca ataagccgtg ggggttttta aatttcttct tgttctttaa
    29161 aatcaatgag tggagctaca taatttgaat ccactttttc aaacgtcact ttttgcatcg
    29221 acgaatgttt tctttcccaa gataacgtaa atagatttga ctctggactt ataaagtcta
    29281 cttcaccttc tagtattact ttcttatgca aatattcctc taataaatat agacttgcta
    29341 aagcatacaa cacattttcc aaatttgctt ccttataatt tgaagaacga ccatgtttta
    29401 ctctgttata tattttccac caatctggtg tatcttcagg ccacccatct ccaaaaggtt
    29461 ttagtgaagt caatgttaaa gaattcataa ccctaacacc attatccaaa cacattaatt
    29521 cgctagattt aataacagct ttaaaatcag ttatgctttt attgctactg ctatcaaatc
    29581 cgcaaagttc tttgaaaata atctcacact ccgaaccaat cgctagtagt agtttttgat
    29641 attcaataga gaatgttttg aaattattct catcgagcat cacaaaatct gtggtattaa
    29701 taaactcatt ttctaaatgt agataatact tccaataaaa atttgtaaac tctgctttgt
    29761 ccattaaatc acctcttttg aattataaca aaaaaaacac gtccacaaca gacgtgttct
    29821 ttgatttcta cacaatcagc ttgccaaaac catccaatca gtcctagcat gcctgtatta
    29881 aaattgaacg ttcatcatct atcatctttc ttagtccagc atgtttgcaa ccacacctac
    29941 aattttcagt ccaacaatag atttcgacta gctaagtctt gcaaaattca ggcagctaac
    30001 ccttcattta ctaccttaat tatactaaat acccacaacg tttacaagtg taatcattct
    30061 aagaaatctg tttagctatg atcgttgtat aatctttaaa cgccgttcct tcattgattg
    30121 aaataatttt ataactcttg tttttccaag ctactttcat atcgtttgtt agttcataat
    30181 cttgatcgta tcgaataata aacgtcaacg ttccttcaag aatggtacct acactagcaa
    30241 gcacgtcttt tagcatttgt gaacgcacat tagcccaaca ttcaaataca gtttcttcac
    30301 tatccaccat ctgcccatct tcatctttta cccgttttgt ctttttaaac ttgactctct
    30361 cagtcaattc atttacattc ttaatgattg gcataataag cacctctcat ttgttgtact
    30421 aatgcttgta ccgttattgg tatccgttcg ctgctggctt cttgacggtt caaataccaa
    30481 tgttgagtaa gtaatgacac cgccaaatca aattgctgat aattatccat atctttatcc
    30541 gttgcgctac tatcaatagc actttttata tattcgcctg ctgtatcaat caattgttga
    30601 ataaagtcat catcaatagt gtgatcgatc cgcatacttt tttttatgct atccaaaatt
    30661 accattttat ctctcctaat gaaaaaaagg cagtgggata aaccgccgcc caatattttt
    30721 ctatactttt ggtgttacaa ctggcgtaaa ctcaatatac actgaagcat tttgatcaat
    30781 tttcttataa tcgttgcgaa cgatcactgc aagtccttgt gagtagtaat cgaatttttc
    30841 ccattgagtc gttacttgat ttctacgagc aacaaagata gattgtgcaa tatcccccat
    30901 aatcataggg aatgttccag ctttaggatt ggcaaacaat gtatctgcaa tcaatactac
    30961 tggcataccg aataatgatt taccactagg tgctgtcaca tcgggttgta aaatgtaacg
    31021 tccatctgaa tctttcaatg tatctaggtg gtaatatccg ctttggttta gaattaccat
    31081 tttatttaat gctgggtcta atgccacatt gtatagttgt ttcaaatcgt ccaacgtagc
    31141 agccgttttc ttagtgaatg tttttaataa atccatgata tgcttattgt cagtgttttc
    31201 taccaatttc gctaactgat ctttgacctc ttgtacaata ttcactgctg aatcttccac
    31261 aacctcattt gataaggcaa tcttaccagc acgagtttct actttataat caactgaagt
    31321 aaacatttca gcgtcaatat caccaatttc agcaagttca gcttttgttg ctaacactgc
    31381 ttgttggtta gttgctactg gatatttacc ttgtccattt gatactgttt ttactgttgc
    31441 atattgagct aagttataat ttgaacgttt caaatcaaag acttcaccga ttacttcttc
    31501 gggaacaact gccgctgcat ttacagtagt gactccatca cgcacttcgc cttgtgaacg
    31561 gatatattct tcatacccac gcaattctgt tttttcgtta tcgattaaag ttttttccat
    31621 gttattactc tccttttgtg ctttagaagt ggattctaag ctattgtttg tttgttttgc
    31681 ttggttgtta ctcataaacg attcgtaaga acgcttattt acttggacat ttgatgaatc
    31741 ataagcggga acggtcacga cactaatttc attaagtgct ttgattttat ctattgatcg
    31801 ttcaattgtg ccatcttctt ttttgtcgaa tgaatctaat ccaagcacga acccaaagga
    31861 catagaatca acaacccctt ttgagatatt ctcatacaca tcatttgaat aggtcgtgtc
    31921 ggttagctct gcttcaaaat gtaatcctgt atcatcgaca ttcaatttca acgtacctgc
    31981 tttgacacta gctagtggct ttgaataatc gtgtccatat aataagaaca catttgataa
    32041 atccacctct tttaatgctt cgggtgtaat cacttctaca aagccgccta aatcttttga
    32101 cggttgcccg aatttcaacg catagcctga aatagttttg ccgtctgttg gttgtttttc
    32161 attttctttc tctactttgg gagaatcggc tgttagctca gcttcttctg tcagccgttt
    32221 ttccttttct tcttcattat tcaactggtg taactccttc ctgttctttt aattgatatt
    32281 tctccaacgt atctaaatac gtaaagttca aacttgctag taaacgatcg ccaccatcca
    32341 tggggggtag ccccattttc gaccgcccct cattaatcgt gagaagtgac cctttgacct
    32401 gatccaatac attttttact tttgtttcag gatcagtctc tagtaactga tcggaattaa
    32461 agcggatatt ggtttcaagt ttcgtatcta attcactcac aaacacatta aagtaatgaa
    32521 tcagcgtact ttggatatac tggagattac tttgaacggt gctagaatgt tcattttcaa
    32581 cgcctaaacg ctcaatcggt acaccaaacg ccttggcaat ttgttttgtg ttccaatcat
    32641 tggaattgat aagcttcaaa acatcggtgt ttatttctaa tgttttgtag tccattgttt
    32701 catcaagaat gatcgttcta agagcattat ctccactact tgaaccgttg gcttcttcaa
    32761 acttttcacg aattgcgttc tttgctgatc cgtctaaatc tgacttatga accttcaaaa
    32821 tacctgaccc actgacacca cgagtaaaga aattgtgcaa tgtgcggttg ccagcttgtt
    32881 gaattttcag ttcatcacgt aacgcataaa gtggtggtaa tcctgttaaa ccatcttggg
    32941 tgaaatattt gaagtgcaaa atatcgctag acttcacacg tcttttttta tcaccaatct
    33001 gataagacaa cgtaccatta tctaattgag taaccgttac acttgagtta ggtaataagt
    33061 gaatttcttc taccttttca ccgttgcgtt taatctctgc aaagctatta ccgtttagca
    33121 acatattgac cgccaaagca aactttaaat gccaaccatc catttctgaa ttaggtttct
    33181 cgtttagtaa cttcaccagt tcattatcag cttgtggcat attgtttttg accaattgaa
    33241 tcggacttga agcaatatca ctagcaataa ttcgaacggc tgcaaacaca tcactatttc
    33301 ttaatgcacg aacgcttgta aagctggtgg tgtaatcatc cgattggatt gataccacat
    33361 gttgtaggaa aggatctccc gttgtatttt gtctcgcttt aaaaaatgcc atcttttaac
    33421 ctcccttctg ttctgtattt agcatgaccg ctaccccaat aagcactatg cccagcgcta
    33481 acaagccata gtagacattt gttagtaagg tgatagcaac caacacacac accaatccaa
    33541 gaagtaaaag aacggtatga atgtaattag aaagaaaatt cttcacttgt ataaaattca
    33601 ttgtctgctt tcgctccttc cgttgtggtg aagtaatcca ttgcaaacac ataggcattg
    33661 attaatgcag ctattggatc aattttgtta ctgttctttg atttattaat ctgaatgccg
    33721 ttgttatccg tttttaaaat agcgttgttc actgcatggg tgagaatcgt atttttattg
    33781 tgaataacgt tgccttcata aacttgttct ctaaaagttc gggtcggaac gtttagagta
    33841 atcgttcctt gtcttacttc taacattggg tagttggctt tttcagcttt actgataagc
    33901 gaattagcgt tatacgggtc ataacaaatc cccatacatt ccaaatcatt ttcttgaatc
    33961 aaatcttgaa taaactgaaa tacttggtca tagtccacga ttccactttc taattgagta
    34021 atggaacact caccagcttt ttctagttct cgataaggca aaccgtcgcg cttttctttg
    34081 tcttgaagcc catatttggt ggctacaaag ctatgtgaat cacaataaag ttcgccgtta
    34141 tctagcggta cgatccatga aacgctagtt aaatcatctg ttttagataa atccactccg
    34201 atataaacgg gcttgcctgt aatatcttgt ggttctactt cggttgcctg ccagtcttca
    34261 gcaatcatat agctgtcctc actcgcttgt ctccataaat tgaagttttt caccaataca
    34321 gaattaaggt tattttgttt caaagcaagg ttcacatcat cttgaatggt tggaatcatt
    34381 acttttttga tttcttcact ttcaaaaatc ggattggctt tgatccattt ctcttgatcg
    34441 tgaatttctt cgggatcgtc caattcccat atcgctataa agtacctgtc ggcttgttct
    34501 tcgcctttta gcacacgatc taacaataag tattcctcgt acataggaac gtttaaatca
    34561 agcccagcgg tactaataac acacaataag ccgttctttt gttgggtcat tcctgactta
    34621 atgacgttgt acgttttacg agtttttgct tcgtgccact cgtcaagaat tgcggttgtt
    34681 ggtgcaaacc catctagcgt gctggtttca ctagccagtg ccatagcgaa cgaattagag
    34741 ggcaagtgct gaatcttaga gttcataatt ttcagttgtg gtcttaaaaa cttactagac
    34801 ttgaccacgt ttctaagtga attagacagc atatcatagc ccaatttagc ttgtttcaaa
    34861 gcgttactta caaacaatac ttgccttgct tctgctggtt cttgttccat aatcaacgca
    34921 ttggcagcca tgccacttgc gatatacgtt ttcccattct tacggctcat actggtaaac
    34981 gctcgattga atcgcctgta attcccttct ttagtacgcc agccataaag actgcctaga
    35041 atgaattttt gaaatcctaa catcttgatt gctttaccgt ccgtagatgg tagcaactca
    35101 atgaatttaa tcgctttggt ggcttgttct tcatcgaaca cataagggaa gctatctgac
    35161 ttagaacgct ccaaatcgct tatatgacgc tcacacgctt ggataatttt cttgggtgca
    35221 atcagcttac ctgataggac tttctcaatg taatcattca tgaattcatc aactctttga
    35281 acgggtcttc gggttcatca tttttaacct tgttcaaagc tagttttgat cgtgattcta
    35341 aggtcatacc tagtgaggtg gcttgttttt tcaaatcact catagcttgt gattgaaccc
    35401 gaaccgctgg attggctttc tttactccgt tttcgcatgt gataaacgta cctgattttc
    35461 ttatctcggc ttcggctgtt ttcattcgag caaaggctaa acaataggct accaatgaac
    35521 cataatctgc ttcactcaat ggataatctt tttttaatgt tggtacgata cgattccatt
    35581 cacttctagc gctcgctgcc atccagtcag ggggcgtgat atttattaat ggttgctgct
    35641 tgaacaattc ttctctagcg tccacacgga ttgctttttc ttcacttgat atattgcctt
    35701 ttgtatcgtg taatatttta gggtgtccca tgttgtcctc ctttcagaca aaagaaaaag
    35761 gaacgtaagc aattaagctc acattccttc gtttttcgat cagaattttt ctttatctaa
    35821 tttatctata taaatgattt ttccgttctg catttgaatg gtcacttgac caaaatgagg
    35881 taactcaatg gtgcgctgtt tccctgtttt atcagtaata acaattgtat ttttcaataa
    35941 atctcctact ttcattatat actttttcac ttgtgattac aagtgtaatc gttatttgta
    36001 attaccctta tgccttttca caacggttat ttgtaccaaa atgagtaggc atcgattgtc
    36061 agtaagtttc acactatgcc cccttaactc aaagggtact cttttctcgt ctttgtcttt
    36121 cattggctgt cttggaatta tggtgtgcat ggcataaaga acgaaaatta gtttcatcta
    36181 accgtctatt ccaatcatct tttagttcaa ttttgtgatc tacaacatca gctttcctta
    36241 tcaagccttc ttctaggcaa tcctcacata ctggatgatt taaccgatac aagtatgaag
    36301 cctttcgcca tgttctactt tgatagaatt gaaaatattt accttcgctt tgctttcttt
    36361 cttcacgctt aattgccgtt tgctttgctt tatgcttccc acaatacttc tgcctataat
    36421 caatcaatgt tttacatcca gcatgattac attgtttttt aactgccata gattaccaac
    36481 caaagcgatc tcgttcgtca gaatcatctt gttcccattt tgatttgtat tcaatataag
    36541 gcgaaactat cttcaagcac gttccgtctt taaaattaac tttagtgatt ttatcttcct
    36601 caaaactaat aaagtctaca tcttcttgag tgtttgaata ctcaatgtgt ttctcaccat
    36661 cgaagtatct aatactttcc atatgcataa ttattctacc tccaataaat tagttatcaa
    36721 ttcatcatct gttagtagca aagccaagaa cttatcatgg atgtttaaaa acaaattatc
    36781 ctcattcgat gtaagctctc gttgtctcgc tcgatcatat aggttaagca gacaattaaa
    36841 ttcatccatt agctcacctg tatcatcata gttgccacta tctaccaatg atcccactac
    36901 tagcgattga cacaacgcta ctgactcctg cagccaatca ggttcttctt ccattatctt
    36961 taatacttct ggatcaaatg tggaataatc ttctgttacc aatcccacac taaatgccag
    37021 cgtgttcatt agaaaccgat ccatttcatc tgtatattga aagttcggtg ctttgtttgt
    37081 tgaaataata ttgagcattt actttcctcc ttgattcgta acacgtggta acacctacgt
    37141 aacaggtacc cgttaccact caatcccaac ggggacaagg gttctagcgt tttggtaaca
    37201 ggtaacgggt aaaatccagt aacattattc cccagcatat tagctaacta tttcaaaagt
    37261 tttaaggaaa taaggtgtta ccccgttacc aaattgctgt atcccttggg gcagtaaggg
    37321 taaactagta acgggctagg tgttactgac ccgttactac ccgttacttt ttgtactttc
    37381 cagctcgatt tttattttca taggcatttg acttattgta aaatcctcgt cgtttatttc
    37441 tttttggcag ttgcttccgt tcccaaccag gaatactgct aataatcaag gcaatctttt
    37501 tagtttctgc atctgatcga ccattcaata aatcctttgc ttgtttatca aaaacagctt
    37561 ccaaaatatc agcggtgagt acgctatcaa ctaaataaaa ctcttgttgc atagtagctc
    37621 cattatagaa atgctgtaaa tttttctcat tacatttact gtcaggatag ttctgataat
    37681 aagaacgtct cgcccacgct ggagcttctt cccatttata cgggatttcc atttccaaat
    37741 atttagtgat tgcttcttta attggattct ccatcttggc atcctcttga tagtcttttg
    37801 caatttctaa gacttcttta tcttcatcgg gactaaaata gattcgttgt cctttatcaa
    37861 aaagcacctt tgcttcagct aacacttgaa gaaaatattc atcctttgtt ttaaacggat
    37921 tcatctttgg cttattctta caaggcaaag gataaaaacg gcggtttcct gtatcatctt
    37981 tcaaatactg cccatcattc gacgttccaa taaatacaca ttgccttgcc caagctatga
    38041 cattccgacc ataaggaggt ctaattttat cgatcttggc actgatgaag ttttttatct
    38101 gttcaatttt agttttgttc attgaagata attctccaag ttcaataatt acattgcctt
    38161 gaagctgcat ataatcatct ttcttttcac ctagcgaatc taatgaatcg gtgaaaaaat
    38221 catccgtgta aagtaatgct ggaacggtac tcttaccaat cccttgcggt ccatccaaaa
    38281 ctggaacgat ctcaaacttt attcctggtt tatagatacg tgcgactgat ccgactatcc
    38341 aacgttttgt tacttctcgt gtgtaaaggt tgtcgtctac ccctaaaagg tcaataaaca
    38401 gtgtttccag tctaggctgt tgatcccatt taactttttc gatacgttct tttactgggt
    38461 tgaaaacatt ctttctagcc acatttacta ctgctttgtg tattttgttg tcatcaaaaa
    38521 ctacattgta gttttcttca atatatgccg ttaaggcatc ctcaaaactg tcttcccatt
    38581 ctccagcggt acgattgatc caaggagaat cttttaatag gtgaatgtat ccgctaaatt
    38641 cgttataagc aattgttcca gctaaattgc tatcattggt taaaatcaat tctgcattca
    38701 caacactgtt acttttaatc tgatcgtctt tattgcatct taatcgtgac atccaggttt
    38761 tcaattcaat gatattgtct tcacgctcgc tggcttcggg aaagtctaaa atactatcta
    38821 atttatgctt atgagccatg agctttcccc ctcaaatctt ttttaatcat cgatcgaata
    38881 atttgattca cttctttatc ttttagcggt ggttcattgt acgcatttgc tgtataaatc
    38941 aattctgcta aagtttgatt atctacaccg tgccatagta accaaccgca taacttagtt
    39001 aaatgatcgt ttctactacc ttcgccagcg ccttgaacaa tcatatctaa tttttttgct
    39061 gtccctgttt ttgctacggg tcggtattga gtctttattt cttgcggttt ttttagcagt
    39121 aggttcacta gccaatcggg agctggtgca atattattta gagatacgtt ttcaataggt
    39181 tcatatgctc tcccaacact tcttgaaata atcatagttg gcgctattgg aactccttta
    39241 gtgataatat caatcccgct taaactttta cctttatcat cacgcccgaa gatatcagca
    39301 aatgcacctg tttcttgttg gataattaca ccatcaggaa ttttaaagaa gaaatggatt
    39361 cctttacctg gtgtgatttc cgtataagta gaaggaaatg gatcatacat tgaagcaagt
    39421 tttttataat tctcaacgcc gtttcgtgtt tcagaatgtt gatctaggtc aactaccacc
    39481 atttgatgat cttcaagcat taacccaata tttctagttg catcttccgt ccaccattca
    39541 ttaatttgat tagaatcgtt agttgcatca ttccaaccac tagagccttt tattggaatt
    39601 ttagttccag gagataaggg ataaattgac catcccgact tttgtaattc cgttgctgct
    39661 tgataattat ttgccaataa acctctcctc ctttttcaaa atactggtat aaatcattca
    39721 gacacctcca tgagcttcaa ctgctcaagt tcgtatttca acccatcccg aacaaattca
    39781 gcacctttta attgattctg atactttacc catgtattaa atgttttcac ccttgcttct
    39841 tgcatatcag taatgtttaa ccatgcggtt aaaatctgat tttttagctc gttgatttct
    39901 cgacaaatta ttttgtgttc tgcatgtttt ttgagtacat acaaaactgt tagtttttca
    39961 ttgaatgaat ttccaaagta ggttttcccg atattatttt ctagttcagc gatcccttga
    40021 ttaatttcct ctaattcaag gctatcgagt tggtttaata gttttgtttt gtacatctac
    40081 ttatgccctc cagttcttaa ttgatagtcg tactcgtcat aagcagtgaa gagatataac
    40141 gctaatcctg caaagatagc tatttttgca attaatggta ataagccgaa aattccgccg
    40201 attaaaatgc ttcctataat taagtttcgt ttcataatgg ttccctccga ttttttaata
    40261 tggtataatc ggaacaaaga atatcgggtt ttatcccaat taagttgcct ttatcctgtc
    40321 cgccaagact gttatggata aggctttttc tttgttctcg atcatgttaa tgctcctaag
    40381 ttgtctgcaa ttcagcagaa cgattataaa ttttctcact cactgaaaac tgcaacccat
    40441 atttttcttt aacagtcatt aattctactg tctcatctaa aataggttca cgatcaacca
    40501 acatttcagg agtcatatct ttctttttta ccattttgga atgaccataa atagttgata
    40561 cagctttatt ggctatcaca ttcgctttaa tgtagtctac tggttttggt tcagtaatag
    40621 cgtggcttaa tttagtcatt gcttcttttt ggtgttcttt gtctagcatg cggaatactt
    40681 gaaagccttc aagtcctgtg gactggcgta gttctttgat gatgtcgaaa atccagtctt
    40741 gaaatgcctc ggcttctttt ttacggcttt tgaatgccat gcggtaaata ttttttttcg
    40801 ttgatgatgt ttacatcttg tgttctgccc aatgaatcga tgaccttact agtagtaacc
    40861 ccatctttag gcaaagagtg aatggctcta gtaacctgtt taagacctaa cgcttccgca
    40921 acatctttcg ccactgccca ccattcatca ttaatatcga caaatcttat aatgtgtccg
    40981 ttccaaattt cagttttcat tcaacgtcct ccagttcaag ccctagcaag ctcgctaaca
    41041 actcaacttg ctgatacaaa atttcttgat actcttcaat agaaagtcgt tctttaaact
    41101 ctgcttgtac taaatcgttc aagccacgca atactccgct taccgttggt tcttgtggta
    41161 gcaatttttg atagtccatt tgttcgtaca aacattcaaa cgtttcttct agcgctttgc
    41221 cttccaagtt tgctaattct gccgctgtgt cttctgtaag aattgttgat aatacttttt
    41281 cgattgcttt attttgtgtc gattccatta ttatttcctc ctattaatcc ctttgccccc
    41341 acggcttgcg tttatttttg gtattcttcc ataaagcgat cacattcatc ttgtgaaatt
    41401 tttacgactc catcaactat aatcaacttt aaaccgttag gaacatattt ctgcatcata
    41461 gtattgtatg aaacgttcat atacttagca gcttgtgcct tgttcatata ttttggaaac
    41521 tggtactttt tttctaataa tgaaagtaac gttccttgag tttgctctac ttgttgaaca
    41581 actcgatcgt caataatttt aaaagctgga tcatcaagcg tgagtttgtc aatttttaat
    41641 tccatattta tcccccctcg ttagcataca tttctataca aagattttaa aaaaatgcaa
    41701 gaggctcttg aatacatttc tatacctctt ataaactcaa tataccatca taccttttca
    41761 tttgcaagaa ttttaacata cttttttgaa cttttatgtt tcacacatat ttaaatcgtg
    41821 atatagtaga gaaaatatat gaatggtggt gaaaatgtgg ctagaaaaaa ataccccgaa
    41881 gcagtggaga caggacaaag aattagactg gctagaattg aaaagggact ctcaatggaa
    41941 caacttggag gtaaattatc cccaccggcc agtaaaggtg ccgttagtaa ttgggaaaat
    42001 ggctataatc ttcccaataa caccaggctt aaacaacttt caaaggtatt agatgtatcc
    42061 actacctatc tattaaacgg atcttataca ttaagagata tgcatatgat gcccgaaatt
    42121 aagcgaaatg tgcttgaaaa gcaattgaaa aaaactattg aaaaaaataa ctctataatt
    42181 gattctaatc tttctaatgc tatcaataac atcgaagaag ataacctgac tgtctcacag
    42241 aagatttatt taacaaacgc aataaaatat cttaaaacag gatctcgaat tgaatttcct
    42301 gacgggagta ccccaattgc ttatctcgca gctcttctaa atacgctttt acgatatgac
    42361 tcattaatta atgattcaga aaaaaataca gatgagaaaa aagagtatca agaattttat
    42421 gaaaatgatc ttctaaaaga gttcacagct cttgttactg agctaaataa acactattca
    42481 aaataataat aaactgcctc ctccaaccgc ccccacggct tgctacacta tggagggaaa
    42541 actattatgg caacattcaa gcaatatgaa actaaaaaag gtaaattttg gctctatgaa
    42601 gcctatcttg gaatgaacaa aatgactggc aaacctgata aggttcgtag acgaggttac
    42661 aaaacaaaaa aagaagccca gcttgccttg tcacgcttgc aagtggacta cgataaaaac
    42721 ggtattaaaa aagcgaacaa cgaaacgttt aaagaagttt atgacttgtg gctagaaaca
    42781 tataaaacca ctgtccgaga agccaccttc atgaaaactg aaatcaagtt tagaaagtgg
    42841 attcttccaa aatatggaaa tcttcgtgtg aatgaagtca cggtaaaaaa agcccaacaa
    42901 attgtaaata gctgggctga aactactgac caatacaagg tacttcattc aacttctaag
    42961 cgtattttta gctacgctat caatcttggg attattgaga ctaatccact agacaacgta
    43021 ttaatgccta aaagaaccac cactaagcat aaagaaaagg tcaaagttta cagtaaagaa
    43081 caactgatca ccttgtttga ttacctaaac agcaaacctg aaacttatcg aaacgacttt
    43141 gataaaacgt tactacgctt tctcttttat agcggggtta ggatcagtga agcccttgca
    43201 ttgaattggt cagacattga ttttcaagaa aatacggtca ccatcaataa aacactaagc
    43261 caatccaaaa atggctataa aatcagtgat cctaaaactg attgtagtgc tggggttctt
    43321 ccactagatg aaagaacaat ttctatatta aaaaaatggc agattaatca gcggaaatat
    43381 atgctgacac ttggtgtaac tgatccgact atgatttttt gtggtatcta taaagaacta
    43441 atcacacacc acgctattta tgcaagattg attactattt gtgaaaaagc tatggttcca
    43501 tttctcggtg tgcatgtagc aagacacact catgcttcaa tgctcttgga cgctggagct
    43561 actatggtag aagttcaaac tagattacgg catacaaaaa ttagtcagac gatcgattgt
    43621 tacggtcact tggcaaaaga aacgaaagaa aagactgttg aaaaattagt acaacactta
    43681 aatattgact agatggcata caatatggca tacagtaaaa aataaaaaaa ggagaaaccc
    43741 tatcatatca aggtttctcc tagctttata gtctattctt cttcttccgc catgaatgta
    43801 ttgaatactt cttcaatcat gtcccattct gcgtctgttt cgatttgttc taactcgcct
    43861 tctgttccat cttcgttttc gatgtatgca taagcttgta attctacatc ttcatcttct
    43921 gaaacaccag caggatataa aagaacatag ttacgtccaa attcttcttg tccgtcaact
    43981 gttagtagga tttcatataa tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg
    44041 tgtccttcat ggtcgtggga atggttatgt tctgtcataa gattacctct tttcttttat
    44101 tattaaaata attgcttacc tgagccgtct aaataatttt gtaagatcat cacagctgct
    44161 aatttatcga tgactttttt tcgtttggct cttgaagtat ttgcctgctc tactagcata
    44221 cgttctgcct gaacagtggt taatcgttca tcttgataat caactggcaa ggagaatagt
    44281 tctttgatct tttctccgta tgacatggaa gcttctgccc ttggtccgat ggaattgttc
    44341 atatttttag gtaatccaac tacaaaccgg tctacttcat attgtgctac taattccttc
    44401 aaccgatcaa agccaaactc ttcttctgct tcattgatcc gaatgatctc tactccttga
    44461 gctgtccagc caaaggggtc actgatagca acacctactg tcttagaacc gacatccaag
    44521 cccattactc tcataaattc actccatgat ttgagaggta gtccttgaca attacttcca
    44581 tgatctcatc gcgttcgtga cggcgaatca aatttcgagc atcacgataa cgaggaatgt
    44641 aggccgggtc tccagaaagc aagtagccga caatctgatt gattggattg tagccttttt
    44701 cttccaaagc ccgataaaca atcgctaatg tttcgctcac ttcttttttg cgactatcgt
    44761 caaaaccaaa acgtacggtt tcatctgtaa aacccatgat tctacacctc tttcccgcaa
    44821 ggtaattcca gcttaccttc tacaacctta ttttactcga agaaagggaa aactacaaac
    44881 aaatattcca aacttccgaa taaattaaga aacactattt gtatgttttt ttcctttttt
    44941 ctgtagcttt atcaaaagta ctcttttcat aaagtatacg ctatatttct ttgaatgtat
    45001 gtttttacac aattttttca taatattcat aaaaaattcg aatagctgct actaattttt
    45061 tgtttttgtt gcttgtcctg tctcataatt gatcgtgtag ccatcttgaa cattgtagat
    45121 aaagacattg aattcaattt ttttgtcttc aatgctttct gcttccaatt gcactcccct
    45181 ggctaccagt tcattcccct caaaatgagg agtgacacgg tagcgtacat gatgatttgt
    45241 ttctttgata tatgccgcaa tatcattttc gtgagcaagc atgtatggag cattcaacga
    45301 acgtgtccct gtcatcaagt ttttaatgtt gttattttga ccagtcagct ggaaaccaat
    45361 taaatggctg cgattatata accattgccc gtcacttaat ttcttttgtt tccaacctgt
    45421 cgggtcaata tacaaaggct ctctcttttc agtagggaac aaatcttttc caagcatagc
    45481 attggcttgt cctactctgt tcaatttatc aatatttgaa tagctttccc atgttccttt
    45541 ggaaaggcta agatcctctt ttgtaaattc cggctgattc ccattgattt ctatttcttg
    45601 atgttttcca tcatattctg gcacatccgc tacttgagaa gcttgtggtg tggaatcaaa
    45661 aatagttgta atctgctgaa agatatctga atctttttgt gtggtggaaa caccgaatgc
    45721 ccctgcaatc acagcagcga cgatccccac gatcgccttg gttgtttttt tgctcatttc
    45781 tttactcctt tactaacagc tccctaaatc ttaacatatg ttcccctata aataaatact
    45841 agaaagaaat agaaacagca aaaaataaaa cctgttttaa gatggcttaa aaatacaaaa
    45901 aagagacgga ttcctcgtct ctaatcagtc accactctat ctgtaaggta tttattatta
    45961 ttcaaaaatt ttcttcatac tcaagacaat acactgattt cacaaccgct tggtaaacgg
    46021 tgcgcaaaag cttgcatctg cggtaataag tccatccaag ttaaaaacat aagaagttgt
    46081 ttttgcttgg ctgttcttat tacttgttgc agagcagctt tttcacaacc tattttaagc
    46141 gggtgacggg aatcgaaccc gcgacatcag cttgggaagc tgggatttta ccactaaact
    46201 acacccgcaa tacaaaaaca gtatacgcct cttttattcc ttcctcaagt ttttttattc
    46261 tttacgcttc tggtatgttt tgtagtaaaa taaaactaat caatcaatta ttggagaaga
    46321 tgctatgacg ggaaaagaat tagatctgtt gctggacact tgtttactcg ctgggaaaat
    46381 catgatggaa agcaatgcgg agatgtatcg ggtagaagat acgatgagtc gaattgcttt
    46441 agcttctgga aatttccgtt tggtcagcta tgtcactcaa accggtctct ttattggtct
    46501 tgatcgtacc tcgaccatcc gaatggaaca aattaccaac cgttcgatta atttagaaaa
    46561 agtagtaaaa gtaaatgatc tttcacgtaa atatgttgct tcagagctaa cattagaaga
    46621 actttactgc gaactgcaag caatcgaccg agaccgttct ttctttcctg tctggttgca
    46681 aatcgttagc gcggcagtga tcagtggaac aattatggtg ttatttggcg gaaagatgac
    46741 cgatttacct gtgactttag tcattggagg attgggatac gcagcttatc tatatagttt
    46801 gaaatttttt agaatcaaat ttttatcgga atttttatct tctttattga ttggcatagc
    46861 cgctatctac agcgtacgtt ttggcttagg aagcaatcag gatctaatca ttatcggatg
    46921 tgtgatgcct ttagttcctg gagttcaaat tacgaacgct atccgtgatt tgctagctgg
    46981 acattatgtc tcaggagttt ctcgcggaac agaagcgatg atgactgcta cgatgatcgg
    47041 ttttgcgatt gcttttattt tccagctttt ttattaggaa aggaacacaa gtatggggac
    47101 gatatttatt cagttttctt ttagtttttt agcgactgcg gcatttgccg ttattacaaa
    47161 tgtacctagg cgttcattga tttgttgtgg tctttctggt gcttttggct ggatgatcta
    47221 ctggatctgt gttcaaatag gcggatctgc tgcttttggt tcattgcttg gttctttagg
    47281 agtagcattc atcagcgatc ttttctctaa acgtttgaaa atgcccgtaa cgattttcaa
    47341 tattcccggg atggttcctc tagtccctgg tggtttagct tatcaagcgg ttcgaaatct
    47401 cgttactgaa tcctatcgag aagcggctca ttatgcggtc caagcaatta tgattgctgg
    47461 agcaatcgct ttaggtcttg tattatcaga agtctttaac cataacatcc gtaatttcaa
    47521 agtaaagcgt gacgcaatca tgatgaaacg aaaaaaacgg ctttttaaat aaacgaagtg
    47581 taacaaagaa aaggttgtga aagagacgtt cagccttgag cgatgacaaa atatctcaca
    47641 aacattgcga caagtaaccg caggagcaat aagaagtaat tatgagtatt aaaaaaagtt
    47701 ttgtaaaaat agcttctcac atttttacat aaaattgact taatactgag aaactgactt
    47761 ttgaacaccc tctatctgta aaaaaggggc tgcgacatga tttttgtcac ggcccctctt
    47821 ctttattcaa attttttcca aaacgttcgt tgataataaa cgatccatag agcggaaagt
    47881 attagaacta aaccgatcac aggaatattc actaaccaaa atggataaaa caaagccaac
    47941 attccgtata agacgataag aataatactg atgatcaatg aacccatcat agttgcgact
    48001 aaacccaaac ttcctgaacc tctgttaaat aattggttaa tattggtcca gttcagcagc
    48061 agcaaccgat aatctctagc gaaataacgt agtgacaata agtagcagcc gaataaagaa
    48121 ccaaatagca agctagggat aaaaaacaaa ggcagctgaa aagacagtcc gcttaataat
    48181 gcgatcccac cagttaaaaa actctgcaat atccatccga atctgaactt ttcttttaaa
    48241 tattggttca tagagattgg caaagaacgg ataaacagaa aattttcttg atccaaagaa
    48301 ataagattgc tgataaaaga agtctgatta actgttaaga aagccaatgc gatccctacc
    48361 aggaaaacaa caccgattaa cttattatct aaatcagtta attttatttc gcctcctagg
    48421 gcaaaagcaa gaataaaaat gattggagtt aacaaggaag aagaaagaac ttgcataatc
    48481 aaattaggtt cttttactaa ttgagcattg taactaaata aaagctgatg cacattttgg
    48541 ttctttttat gggttctttt tattttgaac tcactgtttg aatcagtcag ttgctctgta
    48601 agctttggta ctatcagctg ttttacagca aggaaaaatc ccagagccgt gatagctagt
    48661 attccccaac ccaataaacc ttgttgagaa agtggctgac tggcagcatc atagaatggc
    48721 aataatatcg aaatcggatg ccgatctgtt aactcatttt caatagtccc cgtttgatga
    48781 ttcatccaca tgattccagc aaatgcgatc agcatcgagc tgccaagcaa gatactagaa
    48841 actgtttttc tatgtttttt aaacactgct gttcgagcca aaccaaaaac aatcaaacta
    48901 gccagacaaa aaagaataat caaaaataga gaaaacaaaa ggattgagat aatgatagta
    48961 agcaaaatcc ctagatcagc tcgccatccg gttaaaataa acatgactag aagcggaaaa
    49021 acaaaaggaa tgatggtcaa agcaactacc aaaatctttg ctgaaaaaac ctgttcttgt
    49081 ccaaaaggca gaggcaaaaa tacttgcagg tcatttcctt caaaaaagat attataaatg
    49141 actgatatac tttgagttaa acctaacata ccaaaaagtg ccacgtaata agtaaagaaa
    49201 cctggcaatt taacaaagtt catagcaaac atagcaaatc cgtaaatcag taaaaaaatc
    49261 agccctgaaa aaagattttg agacaaaaga tagatagtta atcgctttcc cttttttcct
    49321 ttctctcttg ctttattcgt gagctgtgga ttaacatata aaagattaac tcgaattaat
    49381 tctaataatt gtcgcttata catacggtcc atctcctgtc gcttgccttc cagccatttt
    49441 caaataaatc ttttctagcg tttcatcagg tgagccattc aataattcac taactgcccc
    49501 ctgataaatc aattcacctt tcttcaaaat agccagttta tcgcataact gttgtgcggt
    49561 atccaataca tgtgtagaaa aaatgactgt ttttcctttt gcggcatgag ccttcatcat
    49621 ttctttcaaa tcaaatgctg cttgaggatc aagtccttgc aaaggttcat ccaataccca
    49681 aatatctgga tctggcagca acgtgccgat caaaatcgtt ttttgacgca ttccatgtga
    49741 aaaacttgcg attgtttcat tttgatgact atacatatca aaaagcgctg tcaattctgc
    49801 taaacgcttt tctttttttt gtgtgtctaa ttcataggca gctgaaatca agtcccaata
    49861 ttctccagcc gtcaattgta aaaaaatatc tggggaatca ggcacataac cgattttttg
    49921 tttgatactc aatcgatttt ccgtcaattt cattccatct actaaaatcg ttcccgatga
    49981 tggttcaata atactaacta aacttttaat cgtagttgat tttccggcac cattatgtcc
    50041 taaaaatcca aaaatttccc cttgtcttat cgagagagac aagtctttta atgcttgttt
    50101 tttcccgtaa cttttactta cattgatgaa ttcaatcatt tctgtactcc ttttgtttgt
    50161 tattactttt tattgtaccc ttcgttattg gtggagtaaa tgtcaaaaag agtgcataac
    50221 aaaaaaggat agaaacgact aggctgctac ttgggttgtt ctcttctaca ggaaaacact
    50281 cttctaacag gtaagatatg agtctttgtt ttctttaaaa tgattcattt tctatctttt
    50341 cttgcataaa attctttagt ttatcaatac ttctctttcc catctctagt tgttctccat
    50401 ttgcaaatgt tactatttcg tttactctgc taatagatgt aatgttttca gtattaatga
    50461 tataagcttt taaatcttta cataaatact catattcgag tgatttttta tattttttaa
    50521 ttgtgccgtt tacgagaatc tcttcatctt tcaatactag gcctatttta tttttataac
    50581 ctggcatgct cattagataa aggatttcat cgtaatggat atagtgatcg ctattaccat
    50641 aacggacagt tgtccacctt tttttcgtag catgttgaac aggatagttt tctttttctt
    50701 tcaataagta actttggatg ctttttttat ttttttcata agtgaattct tttatcaaat
    50761 aaccactagg cgtaatcttt cgattaataa cagttattgc tttattcgga tgagacgtta
    50821 gataaacaat tttacacttt tcattctttt ctctaagcat ttcgcctagt tcgataccgc
    50881 tataataggc gttcaaatcg atgtctagga aaaaaatatc tgaggaatag atttcattat
    50941 ttttaatgaa ttcaacaaat tgtataacgt tcaatccctg aacgatctca aatttccaag
    51001 gacttaataa aattctggat tcttccaaaa tttctactag cattttttca aaaaccaaac
    51061 tatcatccaa aataaatata cgcgttacca taaaaagtac ctccactcga tattttacta
    51121 tcaaaatcat ttcatacaac ttttcaacat acatgttagg aataaattgt aattctcttc
    51181 acaatctttt ccaacacctt tgaaaccgat atcaaatact ttcaggatgc tgcttctaaa
    51241 ataaatatca aacaaatgaa tacatcatgc aaaaaaagcc agacaaccga cgtccttgcg
    51301 tgtcagctgt ctgagctatg tttatttatt ttgttctttt aaaaagaacg atacacatac
    51361 tgatttttcg gagcagagac tttctcagcg tttgtaacaa ctacagatgg gatttcacgc
    51421 ttgaatggtt gataatagtc tgattggatg gttccttcta ttttatacca ttcatcattt
    51481 ttaaccgtta ctccttccgg catatgcgtc aacaaaccaa agactcctga atctgccaca
    51541 caatggataa tgccgaatcg gaacaagaag atatctgtat tgctatcgtt cggtgtttgg
    51601 taaacaaatc cttcatagct gatttttttg ccaatgaatt cgctagggta attataaatt
    51661 agctccataa cttctaaata gttttcatct gtgatcttga tagtttgctt atccccatac
    51721 ttttctttca actcattcat ttggtctaag tagtctgatt tgttgaaata aatactggta
    51781 tccggcttta agtattgtgt ctgcatctcc ggatccccta ctgattcttt acttactggg
    51841 aaattaaatc ctttggcttc tacgatcgta gtgtcgagac taactgttgg aaacaacgtc
    51901 cccacgatta ctggcaaagc tagcagtaca taagcgattc cacgttgata aggttttgac
    51961 aacccatggt catgatcgtg ctcgtgtgcc ttttcttctg cttttccgtt tttgacccac
    52021 aaatacagct gaactaaagc caaaagagca gataaaatca ttgaaagaat agctaagtag
    52081 cgataatgga cattgatata ttgatcgagt ttgccagaaa cttgcaagta catcatcaat
    52141 tcgaaatacc cagataaaat caaaaaccgc agcatatttt ccctcctaaa taaataatgt
    52201 gaatccaata acgctaaggg caatcagtcc gatcagcgat aaaataaagc gtcctgtaaa
    52261 atatcgtttc atcatcagta gatttttgat atcgaccatt gggccaaaga ccaaaaaggc
    52321 agctacaggt gctgtgccga acagacttaa caaagaagat cctataaacg catctgcttc
    52381 tgagcaaaga gacatcgtaa aggccaataa cagcatgatg ataatggcta aaatctttgt
    52441 tgagccgagc gttagcataa ttctagttgg taaataagtc tgcatcgcag ctgcgattaa
    52501 tccgccaatg atcaaataac gacctgtatc aaaaaattcg tcgattgaat gtgtcaacac
    52561 atgcccagtt tgatggatca gctgattttt tggttcattc ttctcacttt gttcttcttc
    52621 atggacatga tgatggtgat tttctgctac tttagacgtg attttttctt tcaatacagc
    52681 gtctttttga aaataggcaa gccagacacc aatcacaagg gcaacaaaaa agctgcctat
    52741 cacccgccag cttgccatct gccaagaatt gccaaatgcg ataaaagtgg aaaaaatcac
    52801 gattggattg atgatcggag cagtcagcat aaaagcaaac gctgtgtgaa ctggtacgcc
    52861 ttttttaaca aattgatgaa cgatcggtac gatgccgcat tcacatgaag gaaagaaaaa
    52921 tcctaagatc gaaccagtaa aaatagagaa gatacgattt tcaggaagcc atctcttcac
    52981 acgttctggg gtcaaaaata cttgtaaggc gcctgagata agacagccta ataatacgaa
    53041 aggtaatgct tcaataacga tcgataagaa aatcgtactc atctgcaaaa ccgaatttgg
    53101 aataaaagaa aacatgagtt tcctcctttt tatttctagt tcattcgctt tgcaaatata
    53161 cgcttttatt caaaaaaaac aagttgctct gtaaattttt acacaactct tattttttac
    53221 caaagcactt caattgactt tttctgttat tcccattaaa ttaaggtctc atacatatca
    53281 cttctatcat aaaagggaga ataaaaatga aacaaaccac taacttgact gaagttcaag
    53341 acatactgca acagtcatcc gttgccgtca tttatctttc gatgcctaat tgcagtgttt
    53401 gtcacgctgt tcgtccacga ctagaggaac ttctcacaca ttatgatatt ccggcacttc
    53461 atttggatgc atttgaaaca ccagaagtag ctagtaaatt tgaagtgctg actgcgccag
    53521 ttgtcctgat ttttcatcaa ggaaaagaag tctttcggca agcacggttc atcgacttta
    53581 aaaaaatccg ttatctacta gatgaactga cagcagaaga tgattccctt tcttatgaag
    53641 aaatcttccg aacaaaataa tcgttgtcca aacagaaaaa agcgattgaa caagtgaaag
    53701 ttttgccttc atttgttcaa tcgcttttta tttttattct gctgtatttt cggaatgcgc
    53761 atttgcgtca gaatgcaaga aatagatcaa ggtttgcaat tcattcgtca aatcaacatt
    53821 ttggatcaat acatctgacg gagcattcaa tcgagcagcg gtaaagttca agattccttt
    53881 gattcctgct tccgttaact gattgactac ttcttgtgct ctttcagcgg gtatcgttaa
    53941 aattgctact tcgatttgtt ggatcttgat ttgttctgac atatcttcca ttggataaac
    54001 ggggataccg tctacgatgc gcccaacgat ttcctcattc acatcaaaag cacaactgat
    54061 acgtatgcta ttactttgat ggaatttgta ttttagtaaa gcacttccca agttccctac
    54121 accgatcagc gcaacatttg tcaattgatc gtcgtttaat gtctttgcaa aaaagtgcat
    54181 aagatcttct acatcataac catacccacg ttttcctaat tcgccaaaat aagaaaaatc
    54241 tcgacggatc gttgcactat ctacttgaac ggcttcactt aattcagttg aagacacttt
    54301 tttctttcca gcattattta aaatccttaa atagcgataa tacaaaggca gtcttttatc
    54361 tgtcgccttt ggaataatat gatctttcac gaaatgacct ccacgtattg tctaattaca
    54421 tctttgattt ctccatcata gcagtattta taggaaaaaa tcaattccat tgctcagata
    54481 ttgtgaaatt ttattcttta ttcatttatt tatcatttct gcaaatctcg cagtataatg
    54541 gaatttatgg tagactacta attgaaaaat agttttttat ttgaggagaa ttcacaaagt
    54601 agaaaagagg attccatgat attattacaa gccaatcaga ttgctcgcca ttttggtgca
    54661 gaagtcttat ttgaaaacat acatttagaa atcagtacag gcgctcgtgt cgctttagtt
    54721 ggcagaaatg gcgctggaaa atcgacttta ttaaagatca ttgctggaat tgaagcacct
    54781 gatgaaggaa agatcgctaa aaataaaaca gcgactctcg gatacctcgc tcaggacaca
    54841 ggacttgatt ccaatgaaac agtttggaac gagatgctga aagcttttga acaagttaga
    54901 caaatggaag agcggatgcg cgagctagag attttcatta gcgaaggtac gccagacaca
    54961 cctgcttata gttcttattt aaaagaatac gaccggctgc aacacgattt ttcagatcga
    55021 aacggctatg gttatgaaaa tgaaattcgt tccgtattgc atgggtttaa attcgattct
    55081 tcattttatg atcagaaaat cgatacttta tcaggcggac agcgcacacg tttagcttta
    55141 gctcgcatgc ttttgcaaca ccctgacatt ttgattctag atgaaccaac gaaccattta
    55201 gatatcgaga cactttcctg gcttgagaat tatctgcaaa gctatactgg tgcattattg
    55261 attgtttccc atgaccgtta ttttttagat aaagtagtca atgaagtcta tgaattaagc
    55321 agaagaaaaa tgcgccatta caaaggtaac tattcgaaat atctagattt gaaagcagaa
    55381 cagttagcaa gtgaatggaa ggcttatgaa aagcagcaaa cagaaatcca taaattagaa
    55441 gacttcgttg ctcggaacct tgttcgcgcc tctaccacaa aacgagcgca aagccgcaga
    55501 aagacattag aaaaaatgga ccggctggat cgtcctcaag gggatgaaaa acaagcaaac
    55561 tttctattca gtatagaaaa gccttctgga aatgtagtct tgcaaatcga agatgccgct
    55621 atcggatatg ataatgtcac cttatctgaa ccaatcaatc tagatatccg tcgtcaagaa
    55681 gcgattgctc tagtaggtcc gaatggtatc gggaaatcga cgttgctgaa atcaatcatt
    55741 caccagatcc cttttatccg tggaaaagaa acgctaggga caaatgtcac catcggctat
    55801 tacgatcaag gtcaagctga tctgcataca aacaagacga tcctagcaga attgtgggat
    55861 gagcatccta ccactccaga aaaagagatc cgaagtgctt taggtgcttt cttattttct
    55921 ggcgaagatg tagaaaaacc aatccctctg ctaagtggcg gtgaaaaagc acgggttgct
    55981 ttagccaagt tagcgatgaa caaagaaaat tttttgattt tggatgaacc aacaaaccat
    56041 ttagatattg acaacaaaga agtattagaa aatgcattga tcgattatga cggcacactg
    56101 ctttttgttt cccatgaccg atatttcatc aatcggatcg cgacaaaagt cgtcgaactc
    56161 tctgaaaatg gcagcaaagt ctatctagga gactatgatt attatttaga gaaaaagcag
    56221 gaagaagaag aaatcgccga attgttggca caagatgagc cagaatctgc tcctgctccc
    56281 acgaacaaat ctgattatta ccaaagcaaa gaacagcaaa agcttatccg tagccttcaa
    56341 cgaaaaatta ctcaggttga agaagaaatg gcgcggatcg atgaatggat cgatcagttg
    56401 gaacaagaaa tggtcgcgcc agaaaacctg gaagatcacg taaaactcaa tgagctgaac
    56461 caagatttag aagctgctag acaagatcaa gaaacaaaat tgacggaatg ggaagaacta
    56521 agcttggaat tagaagaata tgaaaattaa cgaaaaaacc aaaacagaaa ccattcatgc
    56581 tgttttggtt tttctttttt tattttatca aacaaaaatt ccatatttac ctttttcacc
    56641 aataagaaga ccaattacta gtcattctca ctaaagcttc taaataatag taatcacccc
    56701 aaatacaagg tgtatttacc ccattatttt tgggcttatt ataaacagac tgtgttaaaa
    56761 agccatcttc ttcttccgtt gtagcatatc tttctttcaa ttgtcgcata attttccaaa
    56821 cagcagactg gtataagttc ttttctgttt tgtctaatag acctgattct attaattcta
    56881 ataatccaca aaccatgatc accatactag atgtatcttt ttcttctctg ctagaatcat
    56941 caaagatcaa atcccaataa caaatggtat cttctggcaa ccgattcata aaataattgg
    57001 ctaatatttt gcttaaatca aaatattttt gttctttcaa ataacgataa cttaatgcaa
    57061 aaccgtaaat cccccatgct tggcctctag cccaacagga attatcacta taaccttgat
    57121 gcgtacttcc atatcttggt tttccatctt tgacatccat ataaaatgta tgataagttg
    57181 aaccatcttc tcttactaaa taatctgctg cattttgaat atgagcctga gcagcttttt
    57241 tatatttttg atttccagtt tcttcactcg cccaatacaa taatggaaga ttcatgcaac
    57301 aatccatgat cattctacct tgttgtgaag gatcactagg atctccccaa gcttgtataa
    57361 ctcctgcctt ctcccaaaaa cgctgaagta atgtttctgc agcatcaatt gaagcttcct
    57421 tcatttttcg atttcccgtt attttataat ctgctacggt tgataaagaa tacaaaaaac
    57481 caagatcgtg atgattagtg ataatttgac agtcaatcct gtggataaaa tctatatctg
    57541 ctttttcagc tgtttgttga aataaaggat cacccgtctt ctcaaataac aaccaattaa
    57601 ttcctgtcca aaatccttct gtccaaccaa tattgtctct taatggataa cgtcctttta
    57661 taggacactc gcttgggaat tgatactgac tgttactgat acgtttgatt aacctctccg
    57721 tgtgtctgcg acaacgctcg atctcattat tccattcttt ttccattaat tctttataac
    57781 attcatttat ccatttttct ggatgtatta tctcttcaaa catcacttta tcaagcattt
    57841 ccccaccagc ctttagcata attttagtct ttttttattg tttttatttt ggatagttag
    57901 cccggttgtc ccatcaaaat caattttggg acgattgttc catacttcta aactataatc
    57961 ccgatcttga tgagcaacca ctcctgttcc gagccaatgg gttccttttc caaatgtacc
    58021 ctctaatcca ggaataaaag tgttttccca tgtcatcaag tttgtattgg gcaaacccat
    58081 aatagctgtg gattttcgtt taatagtatc gtcacttaaa cagataatac ctgaaaagcc
    58141 agcttcactc accctatagt tttccttgtc ttcttctatg attattcctt ctttctcagg
    58201 agaacattca atagcaaacc ctgtctcata tagaacatat tctgtatcta attctacttt
    58261 gtgtacgcga atctgccagc cttcaaaagg aataagccaa gttctgattt ttgcctgtaa
    58321 agtaggttcc catgtagagg caagtacctc tttcttacat acaatgtctc gtacattatt
    58381 tcgaacgatg aactgtccat aggttttgat gctctcattc ttaaaactgg tcgtgatata
    58441 tgtgtccgct cgctgaatcc ctagcattga atccattgcc atttctgctt tcaattgatt
    58501 cgctcgcggt atcgaaaaac caaattgact agaataagca aattttaaat atttttcatt
    58561 ggtcaatgta tggaaatttg gccctaattg tcccgcattc aaaaaaaatg tatgtccatt
    58621 atcatgttgt atgagcaaat tggcttctga aagaagttta gttccttcac gaataggcat
    58681 aggcatttct ttagccgtcc aatactcatg gtcttcaggt agcgccaaca ataaaaaaat
    58741 cttattcagc cataatggag ataacgtact attatagggt tcagtcaatg tcaattgagg
    58801 gtaggtataa ccaatattca ggataccttt ttcatcaaat atttcttttt gcatccacca
    58861 tcttatattc cgattgacta accccttgat ttcacctaat ttaaaaggca aaagatcggc
    58921 ccacaacata gctgaccaaa agcttgaaac tgcaaaacga taaatcatgc ttcgaccaaa
    58981 aggcacattt gcaccatctt ccgcaaaaaa atgaatatag tctctagaaa aatcaatagc
    59041 tcggttctta tatatctggc taatgggctt ttctggttct agtacgctat aaatcaaacc
    59101 ataataatga aatgcaaaag gattataata atcttggcga cctcttgaac tgtctcgata
    59161 ccagccattt tgaagataac aattttctat attatcaaat gcttctttca gtgctgattc
    59221 tttaactgga taaccaaatt tttggataat tgttccgaca attatcttaa aaaattgcca
    59281 attaccatct gcacattcat attcaaaaat ttgcgagaac caatgaataa tctggctttt
    59341 ttctcctgta tctaattttt tccataataa ttgttcatga tgatataacg ctaataacat
    59401 tgcgggcatt tctaccatac gctggtctct ttcatgtatt tctccccaat aatcgggatg
    59461 ggaaggatct gttccagctt ttattcctga tagaattaat ttcaaccatc gttcatctaa
    59521 ttggtcaatt gcgggccccg ctccccataa aacccgagag aatccttcca ttcctgcaat
    59581 tttatttcca tatcctaccc cttgatgtat ataatcaatt tgtgcgccac ttgctcgata
    59641 aaacggctgg agtggttgat ttatcttttt aaataagtca aagtaatcct gtctggtcct
    59701 taatagtggt actggatgtt tcattgtatt ttctcctctt tttactacca aatagctaat
    59761 tccgactgtt ctaatcgaag taagccttca ataaaaaaat aatcagaata gatcagtggc
    59821 acattataag tcagttctct atggtatgct tctgctccgc ctccaatgat cccatcttca
    59881 tccatattcc aatttgcata gtgcttttct gtcttttgga gtatattgat agcagcttca
    59941 tgatacattt ttgatcgtac agaggaaaca tatcctgcta attccagaag accgcatgca
    60001 gcgcatactc cagcactggt atcaatattc gaatctttta cagcaaaatc aatgagagga
    60061 atataaccag ttttttcgat tttctgaata aaatttactg ctatcttttc tgctatagct
    60121 agatagtacg gattttttgt gtaacgataa cttaacgcga atccatataa agcccatgca
    60181 tttcctctgc tccaacaaga attctcattg tatccttgcc cgccaagtaa ccgaatatat
    60241 ttccctgatt ctgcatcaaa ttctgcgata tgcccggttg atccatcttt cctcactaga
    60301 tacttagctg ttgtatctgc atgctgccga gcaattttag agaacctcgg atcaccagtt
    60361 tccctcgaag cccaatacag taatggtaaa ttcatcatac tatcaataat catccaacca
    60421 ggtttatggt cattccatgc tactaaatag ttccctgaaa tattatagcg tccagcaagt
    60481 accgtagctg catgtaaccc tagttgtcga ctatgtttgt tttgagactg ttgataatgt
    60541 gcaatagccg atggtaagaa taaaaaacct acatcatgat gaacgtctga aaaatggtac
    60601 agggcagatt ctaataattg ttcttgttgt attgctgcct ctttgaactt ttctcgtttt
    60661 gtcttgtgaa agagctgcca taaaatacca ctccaaaaac cattggtcca ccagtcgatt
    60721 cctctgtcct ttattacgtc tgtatatttt ccgttgtaag aaatatatgg aattttattc
    60781 cccattctgt ctaattccgc atctatcttt tcagaagcta cattcaaaat ttttcttgac
    60841 cattccttcg tctgttcatt catggatatc ccgccattct tttaattttt atgaatgtgg
    60901 agttgctcct agaacgaact agatggttaa cattcgttat tttttttact tatttgcagc
    60961 tccaatgtca ttctccttaa atttagactg cgcccacaca taaatcaaga ccattgggat
    61021 cactgaagtt gctaaaatta gcataattgc gccataatta attggctcac ctgtactaga
    61081 aaccgtcata tttttgataa ttaagggaac cgtatattta tctttatctg aaagcatgac
    61141 taatggaaat agatattgat tccaacttcc cataaagata agtaaaccac tagtaataat
    61201 cgtaggtgat aaaatcggta cagcgatttt cgtaaaaatc acccactcat tggcaccatc
    61261 gattctggca gcctccatca gtgctgtagg aaattgactg ctatattgtc gcatcataaa
    61321 cactgaagtt gtactggcaa tcgaagggat gatcacacct gcataagtat tcgataatcc
    61381 taaacgactc ataatgtaaa ataaaggaat caatgtggta aaaaaaggca ccatcctaga
    61441 tagcataact gtattgaaca acagtttatt ccccataaaa cgatactttg ctaatgcata
    61501 acccgccatt gtagaactgg ctgtagctaa aactgtactg attacagcaa taacaatcga
    61561 attccaaata atcaagggat aattatatct cgccatgatg gcttgccagt tttttaatag
    61621 catatcccca aacttgaaat tgattccgcc acttaaaatt tcttgattac tttgcgttga
    61681 agctacaatc atatataaat atggcatcaa aaatattaag gcaccaaaca caataatgac
    61741 gtacgtaaaa attctgcttt gtatcctttg tttagtcatt tttatcacct ccgaatttaa
    61801 attgaatagc cgataatata acagatacaa acaccatcgt ccaggcaata gcagaggctt
    61861 ttccaaaatc aaccatttca aaacttgttt tatataaatt gactcctacg accaaagctg
    61921 aattttctgg tccattggaa gtcccaaaga atatatttgg gatattgaaa ttacccaagc
    61981 cattgatagt ggctaaaact gtggccatta aaatgattgg ctttagcatg gggatcgtta
    62041 ataaaaagaa ttgccgaaat ttcgatgctc catctatttc agcactttcg tatatctctt
    62101 tagaaatccc ttgaagacca gctaaaaata aaatagtgtt atatcctaca ctaagccaaa
    62161 tcatcgtgat gatcattgat attcgtatcc aaaaaggatt tgttaaccac tgtacaccgt
    62221 taattcctag cgcattcaaa aatccattga ataacccagt cggattgaaa aaagtcatga
    62281 atacggaagc tacagccacc gttgatgtaa tggttggaat ataatagaca gttctaaaaa
    62341 atccttttcc ttttaactgt gctgaattca ataaaacagc aaagtaaata gctaatagca
    62401 tcgttattgg aattgatcct gccatgataa tcaaaacatt cgtgtaagat ttaataaaca
    62461 atggatctcg gaagatagct agataattgt caaatccaac aaattcaaat gagtagccaa
    62521 ttttcataaa actaaaagca aacgagataa tgatgggaat caaaatcgtc acaataaaga
    62581 aaataacaaa aggtgtgacc aatccataag cacttttttt atgagacgta tctgttgctt
    62641 ttttcttgct gttctttatt gtcttgcttg gtaacgaaga ttgcatcatc gttaagtcct
    62701 cctaatattt tttaatcacc agttaatttt acgccgtact tatgagcaaa attttctgct
    62761 tcttttagat atgaattttc aaaatcacct gtttgccaat agttatatgc agcggtagtc
    62821 aaataagacg tagcatctga ataagtggct ggtaaaacag tatttgatgt attttcattg
    62881 attttattca atagttcata gtatttttgt cctccttagt agtcaaactt tttctcagct
    62941 gcttctgttt gataggccaa tgagttagat gtagccacgc ccagctccag tgcgttggct
    63001 aagcattctt catctgtcag cataaatgta agaaattgtt gtgccacttg tggatttttg
    63061 gcatctttag ggacataaaa acttgatcct cctgatactg caccttttcc tggattttct
    63121 tttgaaaaag gaatcagtgg agccatgcgc catttgtcag atgcatcagg aaaattcaaa
    63181 gacatattcg tagcatacca gccgccagca acaaacattg cagattcttg aaatgctttc
    63241 tcagcatctt gtgcaccgta gaaatgtaca atatctgagt cgatcatttc tttgattaac
    63301 tttgcagcat gtttcgcttc tttagtattc agacggatat ttccttcttc atcaagcaaa
    63361 gattgattct gctgggctag catgttgata aacaattctt ggtccccaga ttctggcaag
    63421 gcaattccaa aactaccagt tttttctttt aatgcttttg ccacctggat atattcttcc
    63481 caagaactga tttcttcaaa atctacacct atttcagcaa atttttttgg ttgataataa
    63541 caaagtgctg gggtaaaatc actagcccaa ggaataatcg tttcattact cgtttgttga
    63601 cgcaaaatat tcaatttttg atcataaaac tgatcgccat atttgtcata aaatccatat
    63661 tcatcagcac tataaaaagc atcttgaaat ttttccagca cacctgctgc tgaagtgtct
    63721 gccatggaga taatatccgg catatcttcg tgtgttacca attttggggt gatcatttgt
    63781 actgttgctg ttcccgaagc tacattcaca atttttaagt caatatttgc tccagttttg
    63841 cggttataga tttttgcagc attttcaaca tagtttgtca gtttgccgtt tgcgacatat
    63901 acttctaatg tagtggcttt ttttatcatt ggatctgcga tgtttgctgt tacttcttcg
    63961 ttagtttttc cacatgcact taagctgaat aatagggtaa tgtttagcaa agctatcacc
    64021 aatttgtatc tttttttcat gtctatccct tctttctttt caagttctta tataaaacgc
    64081 tttcacttat gtatattaa
//
LOCUS       DACCXO010000003        62503 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.003, whole genome shotgun sequence.
ACCESSION   DACCXO010000003 DACCXO010000000
VERSION     DACCXO010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.003"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            25..696
                     /locus_tag="IVY70_000139"
     CDS             25..696
                     /locus_tag="IVY70_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7957304.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="IVY70_000140"
     CDS             755..1195
                     /locus_tag="IVY70_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957305.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="IVY70_000141"
     CDS             1207..1875
                     /locus_tag="IVY70_000141"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP7957306.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="IVY70_000142"
     CDS             1939..2562
                     /locus_tag="IVY70_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP7957307.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="IVY70_000143"
     CDS             2618..3004
                     /locus_tag="IVY70_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP7957308.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="IVY70_000144"
     CDS             3217..4608
                     /locus_tag="IVY70_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP7957309.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5219..5587
                     /locus_tag="IVY70_000145"
     CDS             5219..5587
                     /locus_tag="IVY70_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP7957310.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5767..6708
                     /locus_tag="IVY70_000146"
     CDS             5767..6708
                     /locus_tag="IVY70_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP7957311.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6777..7508
                     /locus_tag="IVY70_000147"
     CDS             6777..7508
                     /locus_tag="IVY70_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP7957312.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7652..9832
                     /locus_tag="IVY70_000148"
     CDS             7652..9832
                     /locus_tag="IVY70_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP7957313.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9852..10334
                     /locus_tag="IVY70_000149"
     CDS             9852..10334
                     /locus_tag="IVY70_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP7957314.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10350..11111
                     /locus_tag="IVY70_000150"
     CDS             10350..11111
                     /locus_tag="IVY70_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP7957315.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11242..12576
                     /locus_tag="IVY70_000151"
     CDS             11242..12576
                     /locus_tag="IVY70_000151"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP7957316.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12883..13368
                     /locus_tag="IVY70_000152"
     CDS             12883..13368
                     /locus_tag="IVY70_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP7957317.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13361..14044
                     /locus_tag="IVY70_000153"
     CDS             13361..14044
                     /locus_tag="IVY70_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP7957318.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14170..16815
                     /gene="polA"
                     /locus_tag="IVY70_000154"
     CDS             14170..16815
                     /gene="polA"
                     /locus_tag="IVY70_000154"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP7957319.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16860..17696
                     /gene="mutM"
                     /locus_tag="IVY70_000155"
     CDS             16860..17696
                     /gene="mutM"
                     /locus_tag="IVY70_000155"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP7957320.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17660..18289
                     /locus_tag="IVY70_000156"
     CDS             17660..18289
                     /locus_tag="IVY70_000156"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP7957321.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            18341..18673
                     /locus_tag="IVY70_000157"
     CDS             18341..18673
                     /locus_tag="IVY70_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP7957322.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18985..19476
                     /gene="nrdR"
                     /locus_tag="IVY70_000158"
     CDS             18985..19476
                     /gene="nrdR"
                     /locus_tag="IVY70_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP7957323.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19499..20872
                     /locus_tag="IVY70_000159"
     CDS             19499..20872
                     /locus_tag="IVY70_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP7957324.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20872..21798
                     /gene="dnaI"
                     /locus_tag="IVY70_000160"
     CDS             20872..21798
                     /gene="dnaI"
                     /locus_tag="IVY70_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP7957325.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21927..22367
                     /locus_tag="IVY70_000161"
     CDS             21927..22367
                     /locus_tag="IVY70_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP7957326.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22511..23212
                     /locus_tag="IVY70_000162"
     CDS             22511..23212
                     /locus_tag="IVY70_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP7957327.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23209..24330
                     /locus_tag="IVY70_000163"
     CDS             23209..24330
                     /locus_tag="IVY70_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP7957328.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24345..25577
                     /gene="pepT"
                     /locus_tag="IVY70_000164"
     CDS             24345..25577
                     /gene="pepT"
                     /locus_tag="IVY70_000164"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP7957329.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25709..26617
                     /locus_tag="IVY70_000165"
     CDS             25709..26617
                     /locus_tag="IVY70_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP7957330.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26652..26924
                     /locus_tag="IVY70_000166"
     CDS             26652..26924
                     /locus_tag="IVY70_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP7957331.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26935..27981
                     /locus_tag="IVY70_000167"
     CDS             26935..27981
                     /locus_tag="IVY70_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP7957332.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            28232..30841
                     /gene="clpB"
                     /locus_tag="IVY70_000168"
     CDS             28232..30841
                     /gene="clpB"
                     /locus_tag="IVY70_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP7957333.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(30992..31663)
                     /locus_tag="IVY70_000169"
     CDS             complement(30992..31663)
                     /locus_tag="IVY70_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957334.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(31656..32150)
                     /locus_tag="IVY70_000170"
     CDS             complement(31656..32150)
                     /locus_tag="IVY70_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP7957335.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(32170..33390)
                     /locus_tag="IVY70_000171"
     CDS             complement(32170..33390)
                     /locus_tag="IVY70_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7957336.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            33537..35372
                     /gene="lepA"
                     /locus_tag="IVY70_000172"
     CDS             33537..35372
                     /gene="lepA"
                     /locus_tag="IVY70_000172"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP7957337.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            35568..36479
                     /locus_tag="IVY70_000173"
     CDS             35568..36479
                     /locus_tag="IVY70_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7957338.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            37475..37726
                     /locus_tag="IVY70_000174"
     CDS             37475..37726
                     /locus_tag="IVY70_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957339.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTLVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            37809..38081
                     /locus_tag="IVY70_000175"
     CDS             37809..38081
                     /locus_tag="IVY70_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957340.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            38171..38410
                     /locus_tag="IVY70_000176"
     CDS             38171..38410
                     /locus_tag="IVY70_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957341.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            38557..42840
                     /locus_tag="IVY70_000177"
     CDS             38557..42840
                     /locus_tag="IVY70_000177"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAP7957342.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            42939..43304
                     /locus_tag="IVY70_000178"
     CDS             42939..43304
                     /locus_tag="IVY70_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957343.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            43306..43758
                     /locus_tag="IVY70_000179"
     CDS             43306..43758
                     /locus_tag="IVY70_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957344.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            44372..45682
                     /locus_tag="IVY70_000180"
     CDS             44372..45682
                     /locus_tag="IVY70_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP7957345.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            45705..46016
                     /locus_tag="IVY70_000181"
     CDS             45705..46016
                     /locus_tag="IVY70_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957346.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            46377..47633
                     /locus_tag="IVY70_000182"
     CDS             46377..47633
                     /locus_tag="IVY70_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7957347.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            47630..48175
                     /locus_tag="IVY70_000183"
     CDS             47630..48175
                     /locus_tag="IVY70_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957348.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            48176..48676
                     /locus_tag="IVY70_000184"
     CDS             48176..48676
                     /locus_tag="IVY70_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957349.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            48757..49313
                     /locus_tag="IVY70_000185"
                     /pseudo
     CDS             48757..49313
                     /locus_tag="IVY70_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            49333..49677
                     /locus_tag="IVY70_000186"
     CDS             49333..49677
                     /locus_tag="IVY70_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957350.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            49696..50607
                     /locus_tag="IVY70_000187"
     CDS             49696..50607
                     /locus_tag="IVY70_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7957351.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            50686..51678
                     /locus_tag="IVY70_000188"
     CDS             50686..51678
                     /locus_tag="IVY70_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7957352.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            51675..51893
                     /locus_tag="IVY70_000189"
     CDS             51675..51893
                     /locus_tag="IVY70_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957353.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            51906..52316
                     /locus_tag="IVY70_000190"
     CDS             51906..52316
                     /locus_tag="IVY70_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957354.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            52368..54896
                     /locus_tag="IVY70_000191"
     CDS             52368..54896
                     /locus_tag="IVY70_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP7957355.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            54912..57122
                     /locus_tag="IVY70_000192"
     CDS             54912..57122
                     /locus_tag="IVY70_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957356.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            57130..58221
                     /locus_tag="IVY70_000193"
     CDS             57130..58221
                     /locus_tag="IVY70_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP7957357.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            58242..58784
                     /locus_tag="IVY70_000194"
     CDS             58242..58784
                     /locus_tag="IVY70_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957358.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            59006..59236
                     /locus_tag="IVY70_000195"
     CDS             59006..59236
                     /locus_tag="IVY70_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957359.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKGYPYGPTN
                     LKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            59250..59582
                     /locus_tag="IVY70_000196"
     CDS             59250..59582
                     /locus_tag="IVY70_000196"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957360.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            59627..60175
                     /locus_tag="IVY70_000197"
     CDS             59627..60175
                     /locus_tag="IVY70_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7957361.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            60182..60634
                     /locus_tag="IVY70_000198"
     CDS             60182..60634
                     /locus_tag="IVY70_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957362.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            60731..61066
                     /locus_tag="IVY70_000199"
                     /pseudo
     CDS             60731..61066
                     /locus_tag="IVY70_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(61791..62462)
                     /locus_tag="IVY70_000200"
     CDS             complement(61791..62462)
                     /locus_tag="IVY70_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7957363.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT        21216 a        10111 c        13313 g        17863 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca
     5041 cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt atttattatt
     5101 aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat tttcccattt
     5161 tcctatggta aactgacaaa gttgagaatg ttagaacaga tcgtttagga ggaaacagat
     5221 gtttacgaat gaaataaaaa tcatgcttta tgtggacgat gtagaaaaaa atgcaacgtt
     5281 ttggagagca gttggctttg cggaaaaaga tcgtcaagaa atggatggga cgcttataat
     5341 tgaagtagga gttgaggaca gtcaaacctc atttctgtta tatgatcgag catttgttga
     5401 gtcccattca cctggcacag ctactagtgc cccatctttg atgttttaca gtaaagatat
     5461 ctttggacta tataaaaaaa tgcagacgca atcagtcaca ttaggtgata tggtacagct
     5521 tggagaagaa tatgtcttta actttgctga tcccgatggc aactactttg cggttacagg
     5581 taaataatag aaataaagac tttgtttttc tgtgcgaaac aggaaagcga agtcttttta
     5641 attcaaataa taatattatg taaacaaaaa actttccatg cgtaattata cacaagcaaa
     5701 taaatcgtca aatgcgaacg ccttctttat actgtttagg aacgaaaaga aggaaggaag
     5761 aaatacatgg attatcaagt actgctttat tacaattata caaaaatcga agaaccagag
     5821 gaatttgcca aaaatcattt agccttttgc aaatctttgg atcttaaagg tcgtattcta
     5881 gtagctgaag aaggaataaa tggtactgtg tctggaaccg ttgaaaacac gactgcctat
     5941 atgaaagcaa tgaaagctga tcctcgattt gctgatacga tgttcaagat cgatccaagc
     6001 gaaggacatg cctttaagaa gatgtttgta agataccgtc cagagcttgt aagtttaaag
     6061 ctagaagagg acattgatcc aaaccaagtg acaggtgcct atctttcacc gcaagaattt
     6121 aaagaagcta ttttagatga acagacagtg gtgattgatg cacggaatga ttatgaatat
     6181 gacttgggac attttcgagg ggctgtacgc ccagatatac gcagttttcg agagcttcca
     6241 gactggatcc gcgagcacaa agaagaattt atggaaaaac gggtagtgac ctactgtact
     6301 ggtggcattc gttgcgagaa gttttctggt tggcttgtcc gtgaaggatt caaagatgtc
     6361 ggacagctgc atggcggtat tgctacttat ggtaaggatc ctgaagtcca aggagaatta
     6421 tgggacggca agatgtatgt gtttgatgaa cggatcgctg tcgatatcaa ccaagtagat
     6481 aaacaaatcg tcggtcgcga ctggtttgac ggaacaccat gtgaacgata tatcaattgt
     6541 agcaatcctg agtgcaatcg tcagattctt gcttccattg aaaatgaaga aaaacattta
     6601 ggagcatgta gcatagagtg tgcgaaacat ccgcgcaatc gctatgttca agaccatgga
     6661 atctctccag aagaagcaga actgcggttt acagctttaa ttaaatagaa aacaatagtt
     6721 ttgattgtcg accatttcca atatgcgcta tactagcgtt gaggatggtg atccacatga
     6781 aatcattgat ttatgctatt ggggatgtcc atggcaaaca tgagcttttt gaaaaactta
     6841 ttcactatta tgatccaaag atccatcagc ttgttttatt aggggatctc aatgatcgag
     6901 gtcctcgtac aaaagaatgt tttttattag gaaagcaatt agtcgaagaa acaggagcca
     6961 tttacctccg gggaaaccat gaagaatatt ttttgcaatt tctgcaatca ccggaggact
     7021 ggtatgcgcc ttatatgaga aatggtggaa aagaaacgtt tgaaagcctg attcacccag
     7081 gggcatcgga ggaatactcc ccaactgaaa tctcgatgat gatccaatcg agatattcag
     7141 aactgattaa atttttagtc cagcgcccct tgtattatga atgggaaaat tatctttttg
     7201 tccatgccgg tgtggatttg acaaagaaag actggcgtaa gacaagtcta agagacttta
     7261 tttggataag ggaaccattt catgaaggga aaaacaatac aggtaagaca attgtatttg
     7321 gacatacgat tacaccaatg ctccatggag atatgcagac aacagacctc tggattcagg
     7381 atcataaaat cggaatggac ggtggggctg tattcggagg gtcattgcat ggggttgttt
     7441 ttgacaagga aggtattctt caagatattg aatatcagaa caacgaagga ccttggcatg
     7501 gaatctgact aatggaaaga atgaaggagc tgtgactttg ttgcggctct ttttattttt
     7561 atagagaata ttgtttcttt cagtagttta acccaggata aaaagtatga tagaataaag
     7621 aagcaatttt tagcagaaaa gaggcatttt aatgacggaa aatttgaacc agacgatcat
     7681 tcagcttgta caaaaagaac tttcagacta tcgtcctaag cagttgacta ctgtacttaa
     7741 tttgttaaat gaaggaaaca ctgttccttt tatcgcgcgt taccgaaaag aaatgactgg
     7801 aagtcttgat gaagtccaga ttcgtgaaat cgaagagcgg tatgcctatc ttgaaaactt
     7861 agaaaaacgt aaaactgaag tgattcggct aatcgatgaa caagggaaac tgactcccga
     7921 gttagaagca gaaatcaccc aagccgtgaa aatgcaacaa gtggaagatt tgtatcgtcc
     7981 atacaaacaa aaacgacgga caaaagcaac gattgctaaa gaaaaaggac ttgaaccact
     8041 cgcgaagtgg ctgatgcagc ttacagatgg agaggtgcaa tctgaagcag aaaaatatat
     8101 tgacaaagaa aaagaagtct cttctgccga agaagcgttg cacggtgctc atgagattat
     8161 tgctgaacaa gtcagcgata atgcaaaatt tcggacttgg attcggtctt atacgtataa
     8221 caaaggtatg tatgtcagca atgtcaagga tgagcaagca gatgaaaaag gcgtatatga
     8281 aatgtattat gactttgcag aacccgtcca taaaatggtt tctcatcgaa tattagctac
     8341 taatcgtgga gaaaaagaag atattctgaa agtattcttt caagtagatg aagcagcaat
     8401 tttagcttac ttggatcgcc aaatagtcaa aaatcctgcc tctccttctt cttcattcgt
     8461 cagagaagcc tatcaggata gttataaacg ctttattcag ccagcaatcg agcgagaact
     8521 gcgcaatgaa ttgactgaaa aggcagatga gcaagcaatc gctattttcg gcgaaaacct
     8581 ccgtaatctt ttgttgcaac cgccactaaa aggtaaagtt gtcctgggat ttgatccggc
     8641 ttatcgtact ggatgtaaac tagcagtcgt agatgcgaca gggaaagttt tagcaatcga
     8701 agttatttat cctcataaac ctgcagcgca agcaaaaaga gaagctgctg gaccagcttt
     8761 tatccaatta attaataaat atcaagtaga tatggtagct atcggaaatg gtacagccag
     8821 tcgggaatct gaactttttg tggctgagca gttgaaatcc gccgaccata aaacctatta
     8881 cgctatcgtc aacgaagcag gcgcttctgt ttactccgct agcgaaattg cacgaaaaga
     8941 atttccgcat ctacaagttg aagaaagaag tgctgtttct attgcccgtc gcctgcaaga
     9001 tcctttagct gaattagtca aaatcgatcc taaagctgtc ggagtaggcc agtaccaaca
     9061 tgacgtttct caaaaacgtt tggctgaaca attggatttt gttgtggaaa cagcggttaa
     9121 ccaagtaggt gtggatgtga atacagcaag cccgcagctc ttgcagcata tatcaggatt
     9181 gaataaaaca actgcccaaa acattgtcat ttatcgtgaa gaaaacggag aattcaccgc
     9241 acgaactcaa ttgaaaaaag tgccacgctt aggaccaaaa gcatatgaac aagcaatcgg
     9301 attcttgcga gtccctggtg ggaaaaacat tttggacaac acaggtatcc atcccgaaag
     9361 ttattctatt gcaaaagaaa tattaacttc agtccaactt tcagaaaaag agcttggtac
     9421 agaagaagca gtagaaaaat tgacacgatt atccgtagag aaactggctg aaagcctatc
     9481 agtcggagaa gaaacgttag ccgatatttt agctgggctg acacaacctg gacgagatat
     9541 gcgtgatgag atgccagcgc ccttattacg tacagatgta ttgagtatgg aagacttgaa
     9601 accagggatg gaattgactg gaacggtccg taatgtcatt gacttcggcg catttgttga
     9661 tatcggtgtg aagcaagacg gacttgtcca tatatcgaaa ctcagcaaaa aattcgtcaa
     9721 gcatcctaca gatgtcgtgt cagtaggaga tatcgtaaca gtttggatag aacaagtcga
     9781 tacgaaaaaa ggcagaatca gtttgacgat gctttcccct tatgaagagt aacaatgagg
     9841 agtaacaaaa attgacacaa gaagaactgc agcagttagt agaagagata tcattaaaaa
     9901 gcttccagct tcctttcaag caccaagcaa tgttcaatcc acgcttacgt acaactggcg
     9961 ggcgctatca tttaaacgat catcatttag attttaatgc gaagctattt gaagagtcag
    10021 acgaagagac gattgcagga atcatcaaac atgaactttg ccattatcat cttcatctag
    10081 ctggaagagg gtatcagcat aaagatatgg atttcaagta tcttttgaaa aaaacaggag
    10141 ggttgcgcta tgcaccagcc ttgacgaaga aacagataaa gatggagtat tatcgatgcc
    10201 aagattgttc tgccacgatc tgccgcaaga gaagaatcga tgtacgcaaa tatcgttgtg
    10261 gaagatgtca tggagaaatc aaatggattg agacaaaaag agaagacttt aatgaagcac
    10321 aaaaaatcag atgaaaggat agaaggcctt tggaaaaaag tgagcagagg aatctaaaag
    10381 actggaatca gctattaaat tttttagccg cacctgaaga gggaaatttt gaggaactga
    10441 cggcacctac acttatttta gctggaaact gtcttcctat cttagctgat aaagcagcac
    10501 aaatgtattt aaatggccaa gtagatcaat tgtttttggt cggaggggtg gggcatgcga
    10561 ctagaatcct ttatgagaat tttgaaaagc aaggatttca ttttgaagat ggcatgagtg
    10621 aaagtgaaat ctgtcgtcag tatctaaaag aagtctatga tttgcccgat aaggcttttc
    10681 tgatagaatc gaagtctaca aattctggag agaacgccac tttttcactg gagattcttc
    10741 attccttagg agctgtacca gaaaaaatct tattgatgaa tgatccgaca ctgcaacgca
    10801 ggactcgagc aacttttgag aaagtatggc aaaatgaaca aactattttc gcgaatgctg
    10861 tgccatttgt gccggaaatc cttcactttt ctgaagagat catctttaca gcaaaagaac
    10921 tgaatcatca atggcctaag gaatatttcc atgcgctagt attaggagaa atggaacgtc
    10981 tccatgacga tgagaacggc tatggtccga aaggaaaaga ttttattcct cacattgata
    11041 tatcagagga agtttggtct tcttacacca gaattaaaga gtcaataaaa acggattttt
    11101 ctcgtacttg accattttag caagcgaatc ctcctttcca aataaatttg gttgaaattt
    11161 tcaatcgttt ataagataat gaggagcgtg ttgaatgtaa tagctaaaag aatggacgct
    11221 agataagaga agaggattca aatggaaaat caaaataaat tgtatcattt tgttggtatc
    11281 aaaggttcag gaatgagctc cttggctctt gttttacatg aacaaggtct aaatgtccaa
    11341 ggatcagaca ttgaaaaata tttctttaca caaagagatt tagaaaaagc aaatatcacg
    11401 attctgccat ttaacgcaga taatgtaaaa ccagggatga cgatcattgc aggaaatgca
    11461 tttcctgatt cacatgaaga aatccagcga gcaaaagaat taggtttgga agtgattcgc
    11521 tatcacgact ttattggtca ttttatccaa aattatacga gtatcgctgt aacaggctct
    11581 cacggaaaaa caagtacgac tggactgctc tctcatgtat tgagtggtgt ccgtccaaca
    11641 agttatctaa ttggagatgg aacaggacat ggtgatccgc aagcggaatt cttttcattt
    11701 gaggcctgtg aatatcgccg tcatttcctt gcctattcac cagactatgc aatcatgacc
    11761 aatatcgatt ttgatcatcc ggattattat acaagtatcg acgatgtatt tacagctttc
    11821 caaacaatgg ctggacaagt gaaaaaagct atttttgcgt atggtgacga tgcttattta
    11881 cgcaaattaa aagcaaatgt gccgatttat tattatggtg tcacagaaaa cgatgatatc
    11941 caagcacgaa acatcgaacg cacaacttct ggttctgctt ttgatgtcta tcatggggat
    12001 gaatttgttg ggcacttcac tgtaccagca ttcggtaaac acaatatctt aaatgcttta
    12061 ggtgttattg cagtagctta tttcgaaaaa ctggatctga aagaagttgc agaagaaatg
    12121 ctgactttcc caggagtaaa acgtcgattc agtgaaaaga tcgtagcaga catgaccgta
    12181 gtcgatgatt atgcgcatca ccctgcagag atcaaagcaa cgattgatgg cgcacgccaa
    12241 aaatatccag ataaagaaat aattgcagtt ttccaacccc atacgtttac gcgtacgatt
    12301 gcattgatgg atgaatttgc agaagcactt gatttagctg ataaagttta cttatgcgat
    12361 atttttggtt cggctcggga agaacaagga aacgttaaga ttgaagacct tggagcgaaa
    12421 atcaaaaaag gtggagaagt catcaaagaa aataatgtat ctccattgtt agattatcat
    12481 gatgcagtag taatcttcat gggtgctgga gatgttcaaa aatttgaaca agcgtatgaa
    12541 aaacttttaa gcagcacgac gaaaaatgta ctgtaataca attactgcag tgtccttata
    12601 aaaatagaaa gagggggagt ggcaaaaatc ttgccaagcc cccttttttg caacttatga
    12661 taaacggtgt tcaaaagctt gcatctgcgg taataagccc aatcaaatca aaaacatgag
    12721 aagctgtttt ttcttggctg ttcttattat ttgatgcagg acagcttttt cacgacctct
    12781 gtaaactata ggaatctgtt aaatcaaggg agcagaactt gccttttacg ctacttttgg
    12841 tacaataatg tatgattaaa aataaactgg agggtttcta tcttgaacat aggtaagccg
    12901 agaaaactag gaaaaacaat aaatgaaatc gaggaaggcg actcgttatc cttaacagaa
    12961 tctattgaag ataaccagct tttgctgtat ttgggattga cgaatgatgc aaatcctttg
    13021 tatatccaac atgactttgc acaaaaaaca gaatatcaac agccaatcgt tccatcagtg
    13081 atgctgatgg ggatcatcac aagtgcaatt tcaaagcatc ttcctggacc aggatcacat
    13141 gttgtcaatt tttctgtcaa ttttattgaa ccggttttcc attatgaaac actgactttt
    13201 cagtttgagg tcataaaagt agataagatg aaggatgtgg tgacgatttc tgtagaagcg
    13261 gagaatacga acgaagatcg tgtgttggat gctgtcgtta tggtccaacc gccacaagtt
    13321 cctgaattgg aaatagcaat cgaaagtgaa ggtgaattga atgaataatc caagtacatc
    13381 aatgtcaggt atcaatcgtt tttatgcaaa agtttatgga tttttaggta tgggacttgc
    13441 ccttagtgca gtcacttctt acttggctct ttgggtattt cctttgcaat tagcaacttt
    13501 tatcaataat tttccactag gctttgtggg tctgtggatc gttgaactga ttttagtaat
    13561 ggttctaggc gtaaaagcac aaaagaatcc gagcttagca atcggtggat ttattgccta
    13621 ctctcttttg aatggcataa tattagcggt tactcttgca tactacgata ttggaacagt
    13681 aacgcaagct ttcttgacag ctactttgat gttcatgggg atgtctgttg tcggagtaac
    13741 aacaaaaaga gatcttagcg gggttggaag agcaggacag gcagctttaa tcggagtgat
    13801 cattgcacta gtcttgaaca tcttcatttt gagaagtagc gtagttgagc tgttcatctc
    13861 cctactgtta gtcgtcatct ttgcagggat tacagcttat gataaccaaa gcatccgtaa
    13921 tatctatgtc cagacaaacg gaacagcaca tagtggaatt gctgtattca tggcattgaa
    13981 gctgtatttg gactttatca acttgttctt ggcgtttctg agaatctttg gacggaacaa
    14041 ctaaaaaaga aaaaaattca tcagttgggg ctataccgaa ttatttggta tggtcccgct
    14101 ttttcttttc gcattccgca ggattctttg ttagaatgaa taagaacttt acagtaagga
    14161 gttaagaaga tgacaaaaaa taaattatta ctcgttgatg ggaacagtgt ggcttttcgc
    14221 gcattctttg cgcttcacaa ttctcttgaa cgtttcaaaa atagaaacgg tcttcataca
    14281 aatgcaatct atgcattcaa tacgatgttt gaaaatgtta tgcaaaaaga gcaaccgact
    14341 catgttctgg ttgcgttcga tgcaggaaaa acgacttttc ggacagaaat gtatgcggag
    14401 tacaaaggtg gaagatccaa aacacctggc gaattcaaag aacaaatgcc atatatacgt
    14461 gatttgttga caggactagg ggtacagtat tacgaattgc ccaattatga agcagatgat
    14521 attatcggta ctttggcaga aaaagtagac aaagatcaat ttgatgtcgt cgtgctttca
    14581 ggtgatcggg atttgactca attagctagt aaagaagtaa aagtggatat cactgtaaaa
    14641 ggtgtcagcg atatcgaaag ctatacacca gaacatgtag cggaaaaata tgatgggttg
    14701 acgccaaaac agattattga catgaaggga ctagccggag atgcctctga caatatcccc
    14761 ggtgtgacta aaatcggtga aaaaacagct atcaagctat tgaaacaata tggctcagtc
    14821 gaaggaattt atgaacatat cgatgaaatg aagcaaagca aaatgaaaga aaatctgata
    14881 aatgacaaag agcaggcttt cttatcgaaa aaattggcga ccatcaatac ttcagcgcct
    14941 gtggatgtct ctatcgactc attgaagtat gaagggaaaa atctcgataa actcgttccg
    15001 ttttataaag aaatggattt caatcagttc ctttctaaat tgaatatcgt agaagaagaa
    15061 gtaaaaatgg atgatatttt gtttgaagtc gttcatgaat tcaaagagga aatgtttact
    15121 acggatatgg cgttgtacgt ggaaatgatg ggcgacaatt atcacacaga ggaaatcgtc
    15181 ggagtagcgt gggggacaga aaagaaaatt tatgtcacga acgatctatc tgtattcgaa
    15241 aaccgagcgt tccattcttg gatcactgat ccgactcggc tgaaaaaagt atatgatgcg
    15301 aaacgaactt atgttgcttt aaaccgctat actggaaaaa cgaaaggaat cgattttgat
    15361 gtcttgctag gtgcatacct tctcgatacg aatgaaaaaa gcacagatat cgaaggaata
    15421 gctgctcatt acggatacaa cgatatccaa tcagatgaag ctgtatacgg caaaggggcg
    15481 aaaaaaggtt taccagaaga agaggaactc ttttttgctc atttagcgcg aaaagtagct
    15541 gcaatcaatg cgttgacacc aaaattgagc caagaactgg cagaaaaaaa ccaagaagat
    15601 cttttccgga aaatggaact gccattgtct atcattttgg cagagatgga aataagcggg
    15661 atcaaagtag atgcgactcg cttacaagaa atgaagggcg agttctctgc tcgcttaaga
    15721 gagatcgaac aaaaaatcta cgaagaagcc ggcgaagaat tcaatttgaa ttcaccaaag
    15781 caattaggag tgatcttatt tgaaaaaatg ggattacccg ttatcaaaaa aacaaaaact
    15841 ggatattcca cagccgtaga tgtactggaa cagcttcgtg aacaagcacc aatcgtagag
    15901 gatatcctca cctatcgtca aattgcaaaa attcaatcta cctatgtcga gggactgctg
    15961 aaagtgatcc aatcagatgg aaaagtgcat acccgttatg tccagacatt gacccaaaca
    16021 ggacgattaa gctctgttga tcctaatttg cagaacatcc cgattcgatt agatgaaggg
    16081 agaaagatac gtcaggcatt tgttcctaga gaaaaagact ggctgattta ttcttctgac
    16141 tattcacaaa tcgagttacg cgtacttgcg catatctcaa atgatgaaca tttgaaagct
    16201 gccttcctag aaggtcaaga tatccatgcc agcacagcga tgcgtgtatt tggaatcgaa
    16261 aaggcagagg atgtcacacc aaatatgcgt aggcaggcaa aagcagtgaa ttttggtatc
    16321 gtgtatggca tctctgatta cggactttca caaaatctag gaatcagccg aaaagcagca
    16381 cagcaataca tcgatacgta ctttgaaaaa tacccaggtg tcaaagaata tatggaaaga
    16441 atcgtacgcg aagcaaaaga ccaaggctac gtcgaaacgc tgtatcaccg ccgtcgatat
    16501 ctgccagaca tcaattcaag aaattacaat atccgttctt ttgcggaacg aacagctatc
    16561 aatacaccaa tccaaggaag tgctgctgat attttgaaaa tcgcgatgat cgaattggat
    16621 aaacgattaa aagaaacagg tcttcaagct acgatgctgt tacaagttca tgatgaactt
    16681 gtcttcgagg taccggaaaa agaacttgaa tcattagata aattggtgaa ggaagttatg
    16741 gaacaagctg tttctcttca tgtaccactg atcactgaca gcagttgggg aaaaacgtgg
    16801 tacgaagcaa aataaacatt gtttaagaca gagcaatata atgaaggtga aaagaaaaaa
    16861 tgccagaatt accagaagta gaaaccgttc gtaaaggatt ggaacggtta gtagtaggaa
    16921 aaacgattca aaaagttcag gtcttgtggc ccaagatcat cgagcaacca gagacaccca
    16981 tctttgaggc ttctcttgtt ggcgaaacca tccaatccat tggacgtaga gggaagtttt
    17041 taatttttca tctcgatcat tgtgaactga tctcacactt gcgaatggaa ggaaaatatc
    17101 aatttacgaa agaaaataca ccaatcgata aacatacgca tgtacttttc ttttttgagg
    17161 atggaagcca acttcgctac aacgatgtac gaaaatttgg tcgtatgaca atcgtcgaaa
    17221 aaggagcaag tgcaacttac cgaggaatca tgaaattggg acctgagcct ttgccagata
    17281 gcttcttatt agcagatttc gcaaatggac tgaaaaagtc ccacaaagca atcaagccat
    17341 tgctgctaga ccaacgtcta gtgactggtc taggtaatat ttatgtagat gaagcattat
    17401 gggaagcgaa gatccatcca gaacaaccgg cgaacacact gcgccccaaa gaggtagaac
    17461 aattacgtct atcgatcatt gatgttttgg atcgtgcaat agaagccgga ggtaccacga
    17521 ttcgcagtta tttgaatgca ttaggagaat caggcggatt tcaagtagca ctgcatgtct
    17581 atcagcaaac tggcaaacca tgtatacgtt gtggaacgcc gatcgtgaaa acaaaagtgg
    17641 cacaaagggg gacgcactat tgtccgaatt gtcaaaggct aaaaatcgca aagtaggatt
    17701 tgttttaggt ttgacgggtg gaatagctac cggtaaaagt acggcagcca aagtctttca
    17761 gtctcatgga tttcctttga tcgatggcga tgtgattgcc agagaaactg ttgagcctgg
    17821 aacgcttgct ctgaggaaaa ttgtatcagt gtttggccaa gaggtattgc agcctgatgg
    17881 gcaactagat cgagggaaac ttgggatgat tgtttttcca tcaaaggaaa aacggcaaaa
    17941 acttgatcag ctattagatc cgtttatccg aaaagcgata aaagatcaaa tcgcctctct
    18001 aagttccaat catcctttag taattgtaga tatcccattg ctctacgaag gtcattatga
    18061 ccactatatg gatgcagtag ctgtcgttta tactacgcca gaaactcagc ttcagcgttt
    18121 gatgaaacgt aatcaactga cgctagaaca agcgcagcag agaattagta gtcaacttcc
    18181 aatcgaagaa aaaaagcagc gtgcggatat tttgttcgac aacaatggaa caaaagaaat
    18241 acttgtggga caaatcgaaa attggctttt tgctaatcgc tttctttgag tatctgtgtc
    18301 aattttgcta tcctgagtat atagaaagtg agggatagcg atgggatcat taaaaaatca
    18361 aaaggataaa gtcgtaggta acgcaaaaga aaaaatcggc gagatgctca ataaagaaaa
    18421 tctaatcgat gaaggtcgta cgcaatcaga aacagcagaa gaaaaagaaa agcagtacca
    18481 agcaagaatc gatgaaatag aagaccagca ggcagaagaa gaagcgaagc ggcaagattt
    18541 gactcaagaa cgaaaaaatc cgacacctga tcgtagtaaa acaccgctcc cagaaagtga
    18601 ccacattccc gatgagcaaa aaatcgagga acagcgaatg aaacactata caggaatcga
    18661 agagaagtta taaaatagta aaaaatcgag atgtgacttg aagcagatga gaaggtgttc
    18721 atctgcttca agttttttgt gctagatgac taaatcagct tttgtgtaag ttatttctca
    18781 gtaatcaagg tgatcagtat tggaaaattg aaatttagta aggacagggg acacaattac
    18841 gcatgaaagt agaaaaaatt gttcttatat ccgtgttttt tgggtaggac agtcgcgttt
    18901 gtgatttgtt tcgtgttata atacaactag ttgtaaagaa agacacatcc tttatataaa
    18961 atgcgatgca agaggtgaga caaaatgaga tgtcccagat gtcaacataa taattcacga
    19021 gtaatcgaca gccgtcaagc agatgatgga cgtgcgatcc gtcgccgtcg cgaatgtgag
    19081 aattgttcgt tccgattcac tacatttgaa cgaattgaag ctgctccgtt attagtaatc
    19141 aagaaaaacg gggaacgcga agagttcaat cgcgataaaa ttttaagagg actcatccgt
    19201 tcagcagaaa aaagacctgt agctatggaa caaatggaac aaatcgtcaa ccatgtcgaa
    19261 aaccgcgtac gcagtctcgg agaaaacgaa gtatcgacga atctaattgg tgaatatgtg
    19321 atggaagatt tagttgattt agacgaaatc gcttatattc gttttgcaag tgtttatcgc
    19381 cagttcaaag atatgtctgt tttcttaaaa gagttgcaag atatcgtgga taaagcaaaa
    19441 gaaaataata aagagaataa taaagaaaac aattaagaaa caaaaaggag gcatttcctt
    19501 ggaaaatgct tggaaagagc ttcaaccaaa aaatatttac caaggaagaa aagcggctcc
    19561 gctgagagaa gacagcaaga acgccttgct gtatctttat cagccactaa tcggcggaga
    19621 agccttgagt atctatttca ccctgttatc agaaatctct caagaatctg gcaaaggtcc
    19681 tgaagggatg catgcagatc tgcttagtag tctaggctgc ggtatcccgc aattttatga
    19741 tgcaagaaaa aagctagaag gaatcggctt actggatgtt tattttaaag aagatagtga
    19801 tctgggacct cgttttattt atgagctgtt agaaccactc attcctaaag catttttcaa
    19861 agactctgtt ctggcttttc ttcttctgga aaaagttggt gaacgacgat tccagaagct
    19921 tacccaattc tttgaaccta aaaggcatac agcagaggga tatcaaaaaa tcacaaaaaa
    19981 atttttagat gtttatcgct tcaatgaagc agtgtacgct gctaatcaag aaaacatcga
    20041 atcgctgaat aaatcttttg aagaatctga atcagtagca ttacttgaag cagattcgac
    20101 attcgattgg cagtttttga atgattggct ggcaaaaaaa catattgcaa gtcttgatga
    20161 tccaatcatc aaacaagtcg acatgtatca tcagctttac ggtttcgatg aattgacact
    20221 tggcgaactg atcagtcaat cttatgactt tagtgaacaa aaagtatctg aaaaagaact
    20281 gcaaaaagcc attttggcaa tgcagagacg tccggtgaag caaacaaaag aaacagttgc
    20341 ggtttctgaa aacagtaatg aaccctcgtc aaacgaacat ttgacaaacg cagcgcttca
    20401 gctgataaaa gaagcacaga gcattccacc gatgaaatat ctggaagcaa tcaagtcaga
    20461 aaaaggcgga tatgtttcta aacaagaaaa ctggttactg caagatcttg tttctcaatc
    20521 aggtctttcc agtagcgtga tcaatgtttt gctgaattat gtactagtga tcaagaataa
    20581 tgctagctta aatgccagtt atgtaaatac agtggcaaat gaatgggcac aaaagaaaat
    20641 cgccacagca gaagaagcga tccagcacat tcggcaaata agtaatcaag caaaacaatc
    20701 aaaacaaaag aagcaaacaa attatcaaac tggcaagcga aatgttcgac gggaaaagct
    20761 tcctgattgg gtcaaccagc caaaagatga aaaacagatc agcagcgaga aaaaagcaga
    20821 gattgaccgc cgttttaaag aatacctggc aaaaaaggag ggtgatatct agtggaagac
    20881 gtaggcaaag aattgaagag gatcatcagc aaacgcaatt atcaagaaaa gtatgctgaa
    20941 atgatcgctg aagtcttaaa tgatgcggat gttcaagctt ttctaagcgc acaccaagaa
    21001 gaattgacac aagcggatat tgaaaaaagt tacgctaaat tgtatgaatt cgtgcaagaa
    21061 aagcgtaaat atcaaaaaaa cgatccaacg atgatcgctc ccggctatga gccgaagctg
    21121 acgttgaatt ttcattatgt ggatgtcact tatgtaccaa cggaagcatt gttagcacgg
    21181 caaagacaag aagaaatcag aagccgagta aaagcaatgg atatgccaaa agatattcaa
    21241 tcggcttctt ttgaaaactt tgaacgtaca gatggacgag gatatgcgag cttagaagcc
    21301 ctagattttg tagagaaata tactgctgat ccaaaagggt atcataaagg actgtatcta
    21361 gtaggaagtt ttggtattgg aaaaacatat ttattaggag caacagcgag agaattagcg
    21421 attgctggat tcaccacgac gctagtccat tttccgacat ttgccgttga gatgaaacag
    21481 gcaatcggaa aagatcaagt ggcagaaaag ttagatgcag tgaaacgttc acctattttg
    21541 atgatcgatg acattggtgc agatgcgatg agtagttgga tacgagacga agtattcggt
    21601 gttatccttc aatatcggat gcaggaacag ttaccaacct tcttctcatc gaattttaca
    21661 atggatgaat tggaacagca tttgtccgtc actcaacgtg gagatgaaga gccgctaaaa
    21721 gcgaaacgga taatggaacg catacgttat ttgacaaaag aaatcgaaat gactggtcgc
    21781 aaccgacgca atggttgaac agttgacaag tccaactgac atttgaccta gtagtaaaag
    21841 caggtcagcg aatcattata tggacagaaa tgaccggaga caatcaccgc aacaagtaat
    21901 gcagaaactt aaacggagga ttttttatga gggctatttt ttctagtata tctgtagttg
    21961 gtctattttt tcttttagca ggctgcggta gcgcagatag caacacaggt tctgttgatt
    22021 cgattgatgc gggtcaaaca gtaaaaacaa gcagtacgaa aacggagaga caaagcatat
    22081 ttgtcggaac gattgaaagt atcactcaag tagaagcaga agaagaaacg cttcagttga
    22141 aactcgtcga tgtcatggcc aaagaagatc cagcgaatat gggaacgtca ttcagcaatg
    22201 acggagtgat cttaaatgct actgctgaaa aaataagcgt ggacataaat gaattgaaaa
    22261 tcggtgacaa agtccaattt acattagttg agatgccgat catgacgatg tctatcccac
    22321 cgcaagttcc tggagattcg attattgatg tttcattgaa caaataacca ataacatttt
    22381 ttcaaaaata agaaaatgtt gttttataga acgaaaaggt atatttttta aggagatatg
    22441 ggaatttttt tattcctagt ctccttattt tatttggaaa atgctataat tatctagaag
    22501 gagtgaagct atgaaccatt tagatttatc tcagcgctta gccgcagtgg gtgactttgt
    22561 ccctgaatcc gctcgtttag cagacattgg atcagatcac gcttacttac ccgttgcatt
    22621 gatattaaga ggaaaaatcg aatatgcagt tgctggtgaa gtcgttcaag gtccttatga
    22681 atcagcacgt aagcaagtca aaaaagatgg attagaagac cgtatcaccg tacgccttgc
    22741 aaatggtcta gatgctatcc agccacaaga tggtattaca gctgtcacga ttgcgggtat
    22801 gggaggttca ctgatccgag atatcctaga agctggcaag ttgaatcaac gcttaagcgg
    22861 acaagaacgt ttgatccttc agccaaacat cggagagcgt acattacgat cttggctgca
    22921 agaaaatcag tataaaatcc aatcagaaaa aatacttgaa gaaaataaaa aaatatatga
    22981 aatcattgta gccgaaaaag aacaaaaacc agttacttac aatgaaatgg agctgatgtt
    23041 cgggccattt ctgcttgaag agaaaagtga gatcttcaaa tcaaaatgga caagagagct
    23101 tcaacaaaga aaagctatcc gtgaacagct caaacaagca gctaatcaag aggcacgtca
    23161 aaaacaaatc gagcaggaaa tccaaatgat tgaggaggtt ttaaagccat gacaatcagc
    23221 ggacgcactt tcatccaaaa gtttgaaagc tattgtcctc agtggctagc tgaagaagga
    23281 gacccagtcg gactgcatat cggaacattg gataagccaa tccaacgagt aatgatgaca
    23341 ttagacgttc gtccagaagt ggtcgaagaa gcaatcaaga aaaaaatcga tttgctgatt
    23401 gctaaacatc ctccaatctt tcgtcctgta aaacggctgg ttactgatca gccacaagaa
    23461 aaaatgtatg cagatttgtt gaaacatgat attgccgtct atgcagcaca cacgaacatg
    23521 gatattattt gggatggact gaatgactgg ttttgtgaac ttctcggtat tgaagtggag
    23581 agttatttag taaagactca tgagattcat tataaaaaac tggccgttta tgtacctgtg
    23641 gatcatgcac agaaaatgcg tgaagtatta gctgcagctg gtgctggtac acaaggtgat
    23701 tataccggta caagttttac ctcgattgga cacggtcgat ttactccgga agctggtgca
    23761 caaccagcga ttgggaaagt agggaagaca gaacaagttc aagaggcaaa agttgaggtt
    23821 attttgccgg aaactatcga aaaacaagtg attcaagcca tgcggtcagc tcatccttat
    23881 gaggaacctg cttatgatct tttcgctata gatgaaccag tcgaaatgtt tgggttagga
    23941 cgagtaggtg aattgccgca agaaatttct atagaggcat ttgtagaaca agtaaaagaa
    24001 gcgttccaac tagatggact gagaatagtc cagccaaaaa atgcaaaatc ctctgtgaag
    24061 cgcattgcga tttgcggtgg aagcggtgaa aaattttatc cgcaagcaat cgcccaaaga
    24121 gcagatgtct atatcacggg agatatctat tatcatacgg ctcatgatat gcaaagcgct
    24181 gggttgattg caattgatcc cggtcattat attgaatcgt tatgcaagca acgatttatc
    24241 gagaaatttg aatcttggaa acaagaagaa aattgggata ttaacttttt cgtttctgaa
    24301 acaaatacaa acccatttca atttaattaa aaggagagaa tactatgtac gaaaatcttt
    24361 tgccacgttt tttacgatat gtcaaaacag aaacacgctc cgatgcaaca agcaaaacga
    24421 ctccatcgac tcagacacaa gttgcttttg cgcaagaatt gaaaaaagaa ttagaagagt
    24481 taggcttagc tgatgttttc tataatgaag ataacggatt cgttatggga acattaccaa
    24541 gcaatgttga tcatgaagtg cgttccattg gatttatttc ccacatggat acagcagatt
    24601 ttaatgcagt aggagtcaat ccacaaatca ttgaaaacta tgatggcgag tctacgatcg
    24661 tactagatgc ggaagaacgc tatacattga atacaaaaga ttttccaaat ttgaaaaatt
    24721 atgaaggcca cacattgatc acaacagatg gcagtacatt actgggagca gatgacaaat
    24781 cagggattgc ggaaatcatg actgcaatgg agatcttgat caataatcct tctatccctc
    24841 acggtaccat caaagtggct tttggtcctg atgaagaaat cggtgtcgga gcggataaat
    24901 tcgatgttga gcaattcgat gtagattttg cttatacagt cgatggtggt cctttaggtg
    24961 agttgcagta tgaaacgttt agtgctgctc aagcaaatat tacgatccaa gggaaaaatg
    25021 tacatccagg gacggcaaaa gatacaatga tcaatgctct acagttagca atcgattttc
    25081 ataatcagct tccagccgat gaagttccag aaaaaacgga aggatacgaa ggtttcttcc
    25141 atttgatggc attggatggg aatcctgaag aagcttctat gagttatatc attcgcgatc
    25201 atgatcgtga aaaattcgaa gcacgcaaag cacaaatcac acatatccaa gaagagttga
    25261 atcagcgatt tgatcaagaa cgaatcaaag tagatatgta tgatcaatat tacaacatga
    25321 aagaaatcat tgaaaaagat atgagcatcg tggaattagc gaaacaagca atgatcgagc
    25381 ttggtatctc tccagtgatc gaaccagttc gtggaggaac agatggttca aaaatctctt
    25441 acatgggtat tccgacgcca aatctatttg ccggaggaga aaatatgcac ggacgattcg
    25501 aatttgtttc tttacaagta atggaaaaag caacagacgt gattgtaaaa attgcagaat
    25561 tgaacacaaa gagttaaaat cgaaaaagaa cagcttatga caaggcgttc gtcccaatga
    25621 tggaatgtca tctgtctaag gctaaagggc aggacaatgg aattggttgt cccgccctct
    25681 tttttgaaga gtaaatagga ggtaaagaat ggaaaagctg ctagtttttt ataacaaaag
    25741 ttcagggaaa gatgagggtg agaaattagc acgctggttt caagattatg taaaagaaaa
    25801 gcagccagaa ttatatgtag aactagctga aactggccca tcgattgatg atgcggaatt
    25861 agtcaagaaa gcagaagcca tagaagctga tactttagta attataggtg gagacggcac
    25921 gattcatcat atcgtccaag cgttccaaaa aacattatct caataccatg tcggattact
    25981 tccaggtgga actgtcaaca accttgcacg tgttcttgag attcccctca aaaaagaaga
    26041 agctgctgac gtgatcctgg aaggcaatgt acggaaaata gactatggtc aagtcaatga
    26101 tgaagtcatt atctctacac tgacaatcgg aatcttagca gatacagcag tttggatcag
    26161 tcaagaagaa aaacagcgtt acggctcgtg gatctttatc aagaagtttt ttaagttatt
    26221 gctgaagaag aaaaaatatc atttggattt agaaacagac gaggagcatt ggaaaggaaa
    26281 atcccaactg ttgacagtca cgatgtctaa ctccgtgggc ggttttacaa attttgacga
    26341 ctcagcgacc ctagatgatg gaaaatttca tgtaacgatc gtaccttcct tagatatttt
    26401 ccgatttatt ttttatttgc ctagaatcat gagaggcaaa ttctatacga tccccggcat
    26461 tacttatttc acagccaaac gcatgaaaat ccaagcgcag gaaaaatctg tgggcactcg
    26521 aacggatgga gacacgacag atgatcttcc agttgaatta tctgttgtat caaaaggatt
    26581 aacagtttat tcaccaaaga aagaagaaaa agcttagaaa tagtagttaa cagaaagttg
    26641 gaagaagagt aatggatgta ttgttacaca atcctttgtg gttttacctt ttcattgtga
    26701 atttttactt attttgtctg atggggtatg ataaacatca ggctagaact agcggctggc
    26761 gtgttcctga gaataatttg atatttgtca gcctgttagg cggtggtttg ggaggattga
    26821 tctctcgaaa agttttccat cataaaactc ggaaaaagaa gtttactata ggattcgctg
    26881 caggtgtact tgttgatttg attctcattt attttttcca ttaaggagca ggagatgtcg
    26941 tctaaaggaa cgaaaactaa aatcattctg aatctgcttt tgctgattgt gatttttgcc
    27001 attatttatt atttaatcaa tcaatcattt gcagatatct ttgccgaatt gctgtctacc
    27061 agtgttgttg ttttggcagc ggctgttata ttagggacag tttaccaaat cgctgaaggg
    27121 cgctcaatca aagaaattgc tgaacctttc gcgccgaatt ttacaacgaa agatgggttc
    27181 ttgacctcta gctatattgc gttttaccga atcgtttcgt ttggtacagg aacgctgatc
    27241 tcggagattt atttttataa caaaaaagga atgaagctat cccagggagc aggggtgaca
    27301 gcactccata tgattatgta caaactggca gtgatcactt atgcattagc tggcttggtc
    27361 atccaattct ctctttttta ttctaatggt ccatcaatga tctggtttat catcgctggt
    27421 atccttttga cgatcgtgat tgttgccttt ttattggcga tgtccgtcag cttgaatgtt
    27481 caaatatttt ttgtcagctt atcgaataag ttatttaaaa gtgagcgttt gcggaaatgg
    27541 gtggatacat gcaatcagca gatctactct ttaagggaaa cggtccaaac cattatccaa
    27601 gacaggactg cgctattgcg gatttatcta tggaatatgt taaaacttgt attttggtat
    27661 gtacttcctt atttgattct gattgaaaac catcctaaaa tagattttct actgaccatt
    27721 tcgtttatca gctttgcagt agtgctttca ggggttattc ctacgcctgc tggtattggt
    27781 tcctttgaat ttgtctattt gttattattc agaccagttg ttggaacagt tgacgcagtt
    27841 tcttcgcttt tactgtatcg tttcagttcc tttatcttgc catttctcta tggatttgtg
    27901 tatgtgattg ctgaacggcg aagagtgatc aaagaagaaa taatagaagt aaaaaaagag
    27961 aataaagaaa atcaagggta ggtcttttat aagatctacc ctttttatca aatcaaagaa
    28021 aaacgttatg gataagataa ttttacgttt gtttggctct ttttttatat acatacaatc
    28081 aattttgttt ttttaagatt tatataagtc gagaaaaaaa gtcaaaaaac tatgtgtata
    28141 ttttgaccaa aattgaccaa agcgatataa tagtttttgt aaagatcaaa gaaataagtg
    28201 atcttacctt taattaggag ggtatacctg tatgaatatt gagaaaatga ctacgacgct
    28261 tcaagaagcg attgctgaag ctcaacagat tgcggtcact cgaaaacatc aagatatcga
    28321 cattgcccat gtatggaaaa tctttttaca atctaatcat tttggtcgaa atttctatac
    28381 ggatgccgga ttggatgtcg aatcttttga acatgaaatt gatcgtctac tggatgagta
    28441 tccagttgtt tctggtggaa acgtccaata tggacaaaac ctcagccaga atttctttcg
    28501 attattgaat gaagcagacc aaatacgtga aagcttcggc gacgagtttc tttcaacgga
    28561 agtcgttatt ttagcgttga tgaaattaaa aaattatccg ttaactgttt atttgaataa
    28621 aaacggatta tctgaaaaag aattgcgtaa aaacatcgaa gagatgagag gaggagaaag
    28681 agtgacctca caaaaccaag aagaacaata caaagcatta gaaaaatacg gcgttgattt
    28741 agtacagcaa gtcaaaagcg gaaaaatgga tccaatcatc ggtcgtgatg aagaaatccg
    28801 tgatgtcatc cgtattcttt cacgaaaaac aaaaaataat cctgttctga taggagaacc
    28861 cggtgtaggg aaaacggcaa tcgttgaagg gcttgctcaa cgtatcgtac gcaaagatgt
    28921 gcctgaaaat ttaaaagata aaacgatttt ttcattagat atgggtgcat tgatcgctgg
    28981 agcaaaattc cgtggtgaat ttgaagaaag actaaaagca gtcttgaaag aagtcaaaaa
    29041 aagtgatgga cgtatcctct tgttcatcga tgaaatccac aatatcgtag gggcaggaaa
    29101 aacagaagga agtatggatg ccggaaatct gttgaaacca atgcttgctc gcggtgaatt
    29161 acatttgatc ggtgccacaa ctttagatga gtaccgtcaa tatatggaaa aagacaaagc
    29221 gttggaacgt cgctttcaaa aggtattagt caaagaacca acagtggaag atacgatcag
    29281 tattctgcgt ggattaaaag aacgttttga gatccaccat ggtgttaaca tccatgacaa
    29341 cgcattagta gcagcagcta ctttatctga ccgttatatt actgatcggt tcttaccgga
    29401 caaagcaatc gatttgatcg atgaagccag tgcaacgatt cgggtagaaa tgaactctat
    29461 gccaacggaa ttagatcaag ttactagacg tttgatgcag ttggaaatcg aagaagctgc
    29521 actgaaaaaa gaatcagatg atgcaagtaa aaaacgcttg aaaaatttgc aagaagaatt
    29581 agctgaactt cgtgaagaag caaatgcgat gaagatgcag tgggaaacag aaaaagaaga
    29641 agtcaattct gtttcagcaa aacgtgctga aatcgataaa gcaaaacacg aattagaaga
    29701 tgcagaaaat aactatgatt tagaacgcgc tgctgtattg cgtcatggta cgatacctca
    29761 attagaaaaa gaattgaagg aattagaagc taaagcaaaa gacagcgaga ttaagatggt
    29821 tcaagagtcc gttaccgaaa atgagattgc tcaagtcgtt ggcagattaa cgggtatccc
    29881 cgtaacgaaa cttgtagaag gtgaacgaga aaaactaatt aaattgaatg aaacacttca
    29941 taaacgagtg attggtcaag atgaagcagt tgatgcggtc agtgacgcgg taattcgttc
    30001 acgggctgga ttgcaagacc caaatcgtcc tctaggttct ttccttttcc taggaccaac
    30061 tggtgtcgga aaaaccgaat tagcgaaagc gttagctgag aacttgtttg actcagaaga
    30121 tcatatggta cggatcgata tgagtgaata tatggaaaaa catgctgtgt ctcgtctagt
    30181 cggagctcct ccaggctatg taggttacga agaaggtggt caattaacgg aagctgtcag
    30241 acgaaatcct tatacaatcg tcttgttgga tgaaatcgaa aaagcgcatc ccgatgtctt
    30301 caatatcttg ttacaagtcc tagatgacgg acgcttgact gattcaaaag ggcgagtagt
    30361 agatttcaaa aatacggttc tgatcatgac cagcaatatc ggttcacagt tactgctgga
    30421 aggcgtgact gctgatggaa cgattccaga agcagtggct gaacaagtca acacgctatt
    30481 acgtggaaac ttcaaaccag aattcttgaa ccgaatcgac gacacgattt tatttacacc
    30541 attgagccta gacaacgtga aaggaatcgt tgacaaaatg gtggcgcaat tggcacaacg
    30601 tctggaacat caagaaatcc tgctgacaat cagtgatgaa gccaaaacgt ggattgctga
    30661 aaatgcttat gaaccagcat atggtgcaag accattgaaa cgatttatca ctaaggaagt
    30721 agaaacacca ctagctaaag aaatcgttgc aggacatgtt atgcctaaat caaaagtaac
    30781 gattacttta cttgatggac aacttcattt caaaacagaa gagctagaag aaatcgtcta
    30841 atcttccatt gagactataa aagaaaaaag gggtctgaca caactagtgt cagacctcct
    30901 tttttcaaat aaacagtgtt ccagaagtag tttcttcgaa aaataagccg aaatttcttg
    30961 aagctgcccg cttctgtcac aacctctttt attaggtatt tttcactttt tctaatttat
    31021 aactttcaaa gtaacgctca tcttttttac tttgagacca tgcaaacaat cccattaagg
    31081 cagcacctat cgtatttgtc agcaagtcgc ccatcgtatc catcaatgct gctcgtccga
    31141 caaacagtgt gccatcagaa gcagcaaaac gttgaagatt catgcctaag aactggtcgc
    31201 ataagaattc ccagaactcc caaaacacgc cgcaaagtcc agcaaacgca aagccgaata
    31261 agaggaacaa ccaaggactg gtttttgata tctcagcatc tttcatcaag taaccaatca
    31321 atccatagcc aagagctgtc aggaccattg gactcaccgc atgtaagatt ttgtcccaaa
    31381 aagagatgat actgatcaaa tgcatccctg tgcctaaaaa gacagagatg aacaagaaaa
    31441 accagtaaaa caaaacgatt gctgctggta ttttaagttt gaaaactttc aaaatgactt
    31501 gtggcatgaa aatcaatgca atccctaata aagattccac gataaatgtg attccttgag
    31561 aagtcgaata tttttgtgtg ataaattcat aaaaattata gcagacaacg aatacaccaa
    31621 aagcaagtaa agctatttgc acctttttga tttctttaat cactgaaagt ccctccatct
    31681 aaaaatcttt ttacttctga aagcaactca atttctgaag aaaccacttt tccgttcaat
    31741 ttgatcaaac ctattgtata taagttcagg tagtgaaatt gattttcagc tgtttcttgt
    31801 agtgcagcga tttttttcgg gtgggttccg ccttgctgtc gagtatcagt atataagccg
    31861 atcactggga tgcctttcgc ataagccaca ccgatttccg atgctacccc ggcatcgatc
    31921 gtcagcccat ctagtaaagc aatcattaaa tcactagcca acactttttc tgtgtcagca
    31981 agagcaatca ttcgactgtc tgcataagca gttttgtcat tgattgcttc attttcttga
    32041 ggaagataga tcgaaagctc agaagagagc tctctgattt ctttaactaa ctgttggtta
    32101 tataacaaat cacttttagc aaacattggt gcggcaaaat aaattttcat ctaaacatca
    32161 tcctttttat caaaaataat cgctaaagta agcttccatg tttggaatga tacgctctag
    32221 tgacttcagt gtttcttcat ctgtttctaa ccgttcgatt cttgccaaat aagaagcacg
    32281 gcgataattc atcatcaagc aagtcaatgt ttggatatcc agtcgaacgg attttcctat
    32341 aggttcattt aagacacgga cttgtccctg ttcatcccat gttaaagcga agacaccatt
    32401 attccaagga gcgacaggat cttcaatgat gaaatgaaaa gcatcagctg ttccaacaaa
    32461 cggaaaccgt tgaaggaatt cttttacatc aacgatccga gccataaaat atggttcgat
    32521 ctgctctttg atctgactat cttcaatcaa aaatgctaaa ggctcatttt tataaatatc
    32581 tccatgtacc cagtagatca tggaaaaatg tgcggaaata aagttccata agccatttcg
    32641 agcttcttga tcaagataga acatttcttt tacgtgaaat atttcttcag ctacccagta
    32701 gaacagaaca cctcttggtt catggttgct atcgtagtag acagcggctg tgcgctcatc
    32761 ttcattttcg aaacgccaat actcttccca atgaaaatca ctgcggaata aagcaccatg
    32821 gttttgttgg gcaaatttgt cataaacgga aaatacgtct ggatgatcaa ctggtaatcg
    32881 ctcaaccata ccacttactg ggatttgttt aggtagctgt gtatctctga ttttaaagga
    32941 aagtttatca gacatgattt cccatccttt acgccgatag taggggatac tgtaaggata
    33001 caaataagag atccattgct tattcttgcg catattatcc aatgcaacga taattagatc
    33061 ttgcatcaat ccatggttag catattctgg ataagttcca actccagtca caccacccat
    33121 tttataacgt gcaccgtgaa tattgacttc acaaggatag atagcgattt gagagatcag
    33181 tttattttcg tggaaccatc cgaacacttt cgatacttcc aaaatcggtt ttttggaacg
    33241 gataaaagct cgtttattct caaacccgct ttcctcgatg tctgcttcag tcacttggaa
    33301 cacataggaa agcaattcat tgaattgatc gatatgttct tgagtgaccg gttttaattc
    33361 aagattttct ctaaaatctt tttgatccat tttttcatct ccatttatta gcttgataac
    33421 agtatagcaa aattctacaa aaaaaacttc tatagctgac taattaaaaa ctctttaaat
    33481 tactgctttt cgttggtata atggaaagtg ctgattatat gaaaagagga gacgctatgg
    33541 atataaagaa aatgaaagaa cgccaagagc agattcgtaa tttttcgatc atcgctcata
    33601 tcgaccatgg gaaatctaca ttggccgatc gtattttaga aatgacgcac actgtgacgt
    33661 ctcgcgaaat gcaagatcag ctgttagact cgatggattt ggaacgtgaa cgtggaatca
    33721 cgattaaatt aaatgctgta gaactcaatt acacagcgaa aaatggagaa acatatatct
    33781 ttcacttgat tgacacccca gggcacgtcg acttcaccta tgaagtctct cgaagccttg
    33841 ctgcctgtga gggtgccgtt ttagtggtcg atgcagcaca aggaatcgaa gcacaaacat
    33901 tggccaatgt ttatttagcg ttagacaatg atttggagat cctgccagtc atcaataaaa
    33961 tcgacctacc cgcagcagat cccgaacgtg tccgtcaaga aatcgaagac gtaatcggaa
    34021 tcgatgctag tgaagctgta ttagccagtg ccaaatcagg gattgggatt gaagatattc
    34081 tcgagcagat cgtggaatat gttcctgcac caagtggaga cttagatgca ccgctaaaag
    34141 ctttgatctt tgactctatt tatgatagtt accgcggtgt tgttctaaat gtccggatca
    34201 cggacggtat ggtcaaaccc ggagacaaaa tcaaactgat gagtaatggc aaaacatttg
    34261 atgttacaga ggtcggggtc ttctcgccaa aagcagttgc tagagacttt ttgatggtcg
    34321 gtgatgtcgg ttatatcaca gcaagtatca aaactgtcca agatacacga gtaggggata
    34381 cagtaacgtt agcagacaat cctgccgctg aagctttacc aggttaccga aaaatgaatc
    34441 cgatggttta ttgtggtctt tatccaattg atacttctcg ttacaacgat ttgcgggaag
    34501 ctttagaaaa actgcaattg aatgatgctg ctttacaatt cgaaccagaa acctctcaag
    34561 cattaggttt tgggttccgc tgtggattcc tcggacttct tcatatggat gtcgtccaag
    34621 aacgcttgga acgggaattc aatctagaat tgatcacgac tgcaccatcc gttatttacc
    34681 atgttaataa aacggatggt acgactgcta cagtagataa cccggcagat tttcctgaac
    34741 ctgtaacgat tcaggatgta gaagaaccat tcgtcaaagc acagatcatg gtcccaaatg
    34801 attttgtcgg tgcagtaatg gaactttctc aacgaaaacg gggagaattc attacgatgg
    34861 attatttaga tgattaccga gtaaatgtcg tctataatat tccgctttct gaaattgtct
    34921 ttgacttttt cgacaaattg aaatcaagca cgaaaggcta tgcctcattg gattatgaaa
    34981 tgtctggtta tcaaaaaagc aagttagtga agatggacat cttgctgaat ggagaaaaag
    35041 tcgatgcatt aagctttatc gttcaccgag actttgccta tgaacgtgga aaagcaatcg
    35101 ttgaaaaact gaaaaaacta atcccacgcc aacaatttga ggtgccaatc caagcagcta
    35161 ttggacaaaa aatcgttgcc cgttcagaca tcaaagcctt gcgtaaaaac gtcttggcta
    35221 aatgttatgg tggagatgtt tctcgtaaac gtaaactctt agagaaacaa aaagaaggga
    35281 agaagcggat gaaacaaatc ggctccgtcg aagttccgca agaagccttc atggccgttc
    35341 tgaaaatgga tgagcaagat agtgtgaaat aacttataat caatcgaaaa acctattaaa
    35401 taaacctttt tgagtagatg caacactgag gattatctgt ataaaagatt ttaaactgtc
    35461 atttggttat cacggcgata ctaaaattaa caaaattgga atatttttct tgaagatttt
    35521 tagctgtcag tatatgatga aagcatctca taggaggtgc ttttttcatg gttgataatt
    35581 caagtggaag ggttcttaaa tctctaagaa aagagaaaaa actatctcaa aaaaagttag
    35641 ctgatttagc aggtatttca caaagtactt tagttaaata tgaaaaaggg tcaagaaaaa
    35701 tccctaaaga cgtagataat actctaagca agattctgaa tatagaaact ctaataaaag
    35761 atgaggaaga taagattgag attctcatcg gtaaactaat tgcatacaga gatatgaata
    35821 agttacttaa caaagagtta gctgataata taggaattag tgaagtatta ctcagttatg
    35881 tgttaaatag aaagagaaat ccgtcaaaag aaatgcaaaa aaaaatcgat atttttctat
    35941 taagtaatga aaaagaaatt ttgaaggaga ttaatcgtga tagtgagata tttagtttat
    36001 caaaagacga taaaattgtt atgggaaaaa gaatacgaga agtccgaaag aaccgggaag
    36061 agacgcttga aaaatttggt aaaaacttta ccatctatac aggcaaaaat gttataagta
    36121 gatgggaaaa aggtattaat attccagata ttgaaaaact aatgaatatt gcctatcttg
    36181 gtaaggtcac tgttccttac cttatgtacg gagaagatta taagaatata cttccaaaag
    36241 atgaaagagt tagcgatttt aaaaagataa attcttttag tatgggatta aggatgagga
    36301 aaattaggaa agattattat ctagaaaggg aggagtttgg aaagcttttt tctccttcaa
    36361 tttcaaaatg gtccatagat aggtatgaga atggtagaga tatacccaat actaatcgaa
    36421 ttatccaata tgcttatata ggaaatcttt ctttagaatt tttgatttac gggatataaa
    36481 aatagtttac gtcgatactt attaagttat cgacgttttt tattatagaa aaaatttata
    36541 aaaggaggtt tcttgatatg ataagaaaca aaaagggttt gttgaaagtt gctttactgg
    36601 cagctttttt ctaagtaact ttttgacaag ctcaatggca acacaagcgg aagaagtaat
    36661 acaagaacta actgttatga ataacagcta gataaaatgg gctatgtatg ttatccaaca
    36721 atttagtaat cactagaagc ctagcgaaag ttcgcttgat aagttataga tgagtattga
    36781 tttgtagtgt ttccaaatca aaagggtaca gagccacatg aaatatgttg tcctctgcag
    36841 cgtcaggatg ggaatccata ccatggagat tcggagatct gtgagggaat acttacgtgg
    36901 ttaatatgtc agttgtgttg gacgtagtaa tggtcatagt gggtagattg actgaattct
    36961 tatatgagaa aatgaaacca acaagaagat agggttattt atacagaatt ggggatcttt
    37021 tctggaaaag taatcggata atgcgagatt cgtacggtct tttttaaatt tctctaaaaa
    37081 aaacggcatc gaatgggttg ggtgattgat aagcaatgaa tcatagactg atatagaata
    37141 aaaaatgatt ctatttatct ttttacatct atcgtttttt caagtagccg caaattatat
    37201 cagcattcaa caatttttgt atacaagttc tagtaaaagg atctgaagaa aaagttggtt
    37261 gttgtgaaga taaaaaaaga atcaggtttt actgagtttt tcttcaaaat ttgtgttatt
    37321 aggttaggac aaagataaga agaagtatga agatttaaaa aaggaatgag gaaaagatag
    37381 taatttccga ttattggagt ttttttattt ataaagaaga agcgaataag ttgtgattcc
    37441 tagatttcgc gcgtgattaa ttaatgagca aaaaatgcgg aaagttttag tgtttaatac
    37501 acgaggacaa agtttcttag gtattgaagg cacacttgta acggttgatg ggtggtgtga
    37561 ttgtttcgag gttggaaaac atgttgaact tatggcattt actgagctaa aagataagaa
    37621 caacgtggaa tttttcgaga gtgatattgg ttgggacgat catgaagaag tttatggaaa
    37681 atttattttg cggaagggaa attcttatac gaatggggaa gcataagtag cgatttgtta
    37741 gagatcattg ataggattga aattgtcggg aatatttatg agaatctaga gttatttgag
    37801 gtgatagaat gagtgatggt tttgaacaag tatcaataat taaaaatcaa gttcgggaga
    37861 ttcttagaaa aaaaggctat ttggtagata gttattttga aggagattac gaaacatggg
    37921 taggtgtcta tgctcgtcca gagaataaac ctacctattt agatcctacg acgtcagaag
    37981 atgcctattt acaaaatagg tatcgagtag atggttttaa gcaagacttt gcggagtggt
    38041 ttgaatggga aattgaaaac ggagaagtaa aagaagaata agagtacttg ttgtatcaaa
    38101 ataagccagt agaacaacca aaccagtagc tagtaatgac aataatactg aggaatcgga
    38161 tcattatggt atgattgata tttctcttcc agaagatctt aagccagatc ctgtaacaat
    38221 tggcaatagt gtcatgattt ttgattcatt agatgaagct atgtcatatg gttggaaaga
    38281 aattaaggat gaaggtagta attggtatgg catgagattt ggtgatggtg aagttgccaa
    38341 aaaagctggt attccaattg ggaaatggac agttgatttc cacgaagcag gttctttttt
    38401 tgttctgtaa ttatagtggg agggtaaaat cattgatgaa atgtgttcag ataagagcgc
    38461 cagataaaaa tagaagagac agttcacgtc gttatcactt ttggtaacgg cgttttgttt
    38521 tattatataa aattctcagg aaaaggagga attcttgtgt atgcaaaaac gaaaatgaat
    38581 tggttgaaag cactcgcatt ggtggctttt tctcaattct gtttcttggt gggaactgct
    38641 caagcacatg cagaagaaac gacggctata caacctacga tggaagccat tgcccaagtc
    38701 gcttctgaag tgacagaagt ccaaccagca gaagccgggg tcccagctgg tgaagccact
    38761 gttgaacagg cccctgtgac agaagcgcca acggtcaatt ccaaaacgac tctcgatcag
    38821 acgattgggg aaacacaaca cccagggaat agtcctggta cttcacaacc agttgaagaa
    38881 actgcctcaa ttacaacccc tgcttcagaa acgtccactc tagaaggaga aaaagctgcc
    38941 gaagcaacgc cttctgtgca gccagagtct tctgagaaac aagcggaaga aaagaaacga
    39001 atagaagaaa cagtggaaca accagcggct actgctgaca aagaaggaaa aaatgaggaa
    39061 aacgttgaaa aaacaatctc ttcaaccgca gctagtcaaa atcaaccaac gaaaccaaac
    39121 aaagaatcta cggctagtat tgaaaatcaa aagaaaaaag agcagaacat tccaaaaaca
    39181 ggtacaccta cactgttagt aacagtacca accaatcgtt cggttcgtgt tcgccgtgca
    39241 accaatgcag ctgttttagg tggtcggaaa attgattggg gcgaaaatat tggtgttact
    39301 tctagtccga acaatctaaa cttcacttta aattatatgg ttcgttttga gattaatgga
    39361 caagtggcgt tttgtattga gcctggtgtc tcagccgcag aaggtcaagt ttatacagct
    39421 actgctttga aaaactattt gaaaaattca aatgtacgga aaaaaacaag cttgatctct
    39481 tatttaggat atgtcacaaa taaagacaaa tccgatgaac aatacattgc ggcacaattt
    39541 atgatttggg aagctttggg cacaaaaatc ccaatcaaaa atatccgtgt tgattatgat
    39601 cgtcgaaaag cagaaattca acgtttgatt aataactttg gtgaaaaagc aacttttaat
    39661 aatcaaacac atacggtaaa aattggcgaa acgttgaaag taaagaataa aaatgatttt
    39721 ttgaaatatg tgaaagacat tcaaaaacca aaaggtgtca atgtatctat tcacggcaat
    39781 gaagtatgga ttacagtcac aaaagaggca ccggataaag caaaaattca attgaataaa
    39841 attaaagttc aaggtgcgcc attagcctat cgtcgtccag gagctcaaac aattggtgtg
    39901 ttacatccaa cagaacctaa tgcgtctgtt attaatttac aaattctaaa acaaggaaac
    39961 attcgtgtgt ttaaagaaga tgcggaaact ggtgtgaaag cacaaggtgc agcttcttta
    40021 gaaaatgcgg tctatggttt gtatcaagca gatgggaaaa agattaaaga aattaccttg
    40081 aaaaaaatta acggcaaagt acaggcagaa attaaagatc ttgatcctgg caaatatgtt
    40141 ttaaaagaaa tcaaagcgcc aaaaggttat gtgctttctg atgaagcaat tgaaattgtt
    40201 gttgaacctg gaaaaactgt caatgcgacc gcaaaagatc aagtgatcaa aggaaccatt
    40261 gatttagtga aagttgctaa taaagattta gtcgattcaa cgaatccaga taacaaacca
    40321 aaattagctg gcattgaaat cactttgacg tcaaaaacaa ctggcaaagt ggtgaaaacc
    40381 gttgtgacag acaatgatgg ttatgctact tttggtaaaa ataccgttgt ctttgatacg
    40441 tacattcttt ctgaaaccaa aggaaaagaa gggtataaac tatttgaacc acttgaagtc
    40501 acaatttctg aaaatgggca aactttccat tatgttttag aagataaagt aattgaacaa
    40561 cgtcttaaag tggtgaaagt ggatcaagaa acaggaaaag tgattccact tgctaataca
    40621 gaattcaaga tttttgatac tttagccaat cgttatgtca caatggagat tccaaatgat
    40681 acagaaacaa cggatatctt taaaaccaat gaaaaaggct ttttcctaac taacggtaca
    40741 cttacttatg gaaaagatcg ctatcgtttg gaagaaatca aagcccccgt aaattacgtt
    40801 ttgaatcaaa acccattagt gttctcgatt accaatgata cagaaccgat caaagtgatc
    40861 cattttgcga accgtcaagc acggaaaagt gtaaaattat tcaagtatga agaatggaac
    40921 aagaagaaaa cgccgctcgc aggtgtgaac tttaccttgt ataacggtaa aaatcaagca
    40981 attggtgaat ataccactga tcaaaacggg gaaattttag tgaaaggtct agttgccgat
    41041 gactattact tcttggaaaa tgccccatta gaaagtttcc aaccaaatac agataaacaa
    41101 gcattcacgg ttgcattcaa acaaaatgcg ccagatgatg gggaaacgtt agtggtttct
    41161 gtacgtaatt acttgattcc accaaaacta gaaacacaag cgtcaaataa agccgatggt
    41221 gaaaaagtgg ttgatccatt agaaacgatc gtcattaaag acagtgtttc ttataccaac
    41281 ttgtttgtcg gaaaagaata caccatttca ggagtattga tgaacaaggc aacgaatgcg
    41341 ccaattttac aagatggcaa agaaatcaca agtaccttgg cctttattgc agacaaagtc
    41401 aatggcacaa aagaccttga atttgtggtt aatgctagtg ttctgaaagg tcaaacgatc
    41461 gttgtttttg aaaaactttt ccgtgatggc attgaagttg cagcacatgc ggatatcaac
    41521 gatgaagccc aaagtgtgaa agtgaataat ccaaccgttc ataccacggc tgtctttgaa
    41581 gacggattga cagtcgcaga cccaacagga ttgtttacct taaacgatac cgttgaaatt
    41641 accgagattc ttccaggaaa agcatacttg gtcaaaggga tcttaatgga taaagcaacc
    41701 ggtgaaccct tgcttgtgca tggaaatacg gtagaaacga gtgttggctt tattgccaaa
    41761 gcagaaaatg aaacggtgat cgtaccattt acctttgatt tgacaggtat aaatggtcgt
    41821 gaaatcgtcg tgtttgaaag tctttttaga aatgatgaag aactagcaaa ccataaagac
    41881 atcaacgaca aagggcaaac ggtacgagta actaacccaa gtgtacgtac aacggctaca
    41941 cataaaccca ctggcttgaa agaggttaat ccattaacga aagtcaccat tacagacgct
    42001 gtgaaatacc acgatttggt cgttggaaaa acatacacag tcaacggtgt tctaatggac
    42061 aaagtaacgg aaaaaccagt gctagtagat ggaaaagaag tcacaagtac cttaacgttt
    42121 gtggcagaaa caacggatgg tacagtcaat ctagacttta ttctagacgc acgtgcgttg
    42181 cgaggcaaag aagtcgtggt ctttgaagat ctgtttagag aaggcgtatt attggcttct
    42241 cacgcagata ttaccgacaa tgaccaaacc atcaaaatca gtaatccaac cattacgacg
    42301 aaagcgacga ataaaaatgg cggaaaagag atcttggcct tgccaaatca aactgtgctt
    42361 gaatgggtca aagtagacgg tcttgcgatt ggtcaaatct atcgcttaat tgttcaaggc
    42421 tacttaacac cagacggcac accatttgaa ggcactcatg cggaaaaagt ctttgcggcc
    42481 gataaagaaa tgatggaatt cttatttgaa ttcttagtgg acggtcgcaa tcttgcagga
    42541 aaaggcttga gttttgcgga atggctccaa gcgaaaacgg aagatgagaa agaaacgttt
    42601 gaagaagtgg cgaaacacaa tgtagatcta acaaacaaag atcaaacggt tcaaattgtg
    42661 gaagcgccaa aaccaccgca accagtgaaa ccagaacaac gggttaaacc agctactgtt
    42721 gtaccaatgt tgccaaaaac aggtgaacaa acaagtagta tacttatggt tcttggatta
    42781 gtagtattag tcattgcttt aggtataaca ctttataaag tacgtaaagc caaagattag
    42841 tcatgaggaa caagcggtta ggatttttaa ccgcttgttt gattgtaatg tagaaatatt
    42901 tagaatttat tttggttttt aagaggagga aaaactttat ggcagaaaaa gcattgcgta
    42961 tgaagaaaga gtttttaggt caagtatttt tattgggttc acgggaacct aatccaccaa
    43021 aggaaattaa tggtgtgtct gggcgatatg ttcaattaca agcagagtgt tcgcgagaag
    43081 aacagcctgc tttttttgtt tatctcacag ctgaagagga taaaagatta cgattaccag
    43141 aaaaagaggt gcgtatttca tttgaagaag cttattttgt ttttcgtaaa gaacgagtga
    43201 atagtcgtga aacaaatgat atgggacaaa cagagcgtcg aacaactggc tatcaagatg
    43261 ttggtgtctt tttaactggt gtaaaaattg gggaggtttt gtaagatggg attaaaattt
    43321 ccaaataatt atgacattac agaagcatta gatgaaactt gtttaggagc cgtacgcttt
    43381 ggcgcattag aagagtctga tcgtgtattt gataatgaaa caggcgatta tacgggagaa
    43441 attcgatcac agcgagcaca attagttttt ggaaatccag atgaaccaaa gattgcacga
    43501 cgagttaaat ttccagcaga tgtagatttg actggtttta aacgacgtga tcctgtgaaa
    43561 ttaattgatc cagaatatca tgtggtatat attccagcac aagatggcaa tcgtgaatat
    43621 tcagatttag tgatttatgc gaaaggatta gagaaagtag aagctccaac aaataatcct
    43681 aatatgaaac aatcacaaga aaaaggttta gatcaaaaac aaccaagtgt tgattctaag
    43741 gaaaaagaga aaaaataagg tttttttgaa agaataggaa ggagtcttcc tattcttttt
    43801 atctaagata gaaggaaggg gagattttga attcagataa aaccaatcaa atactttgag
    43861 agattgtgct tgttatcaaa atatccaaca attgattgag tattttggag acaatccaac
    43921 gatttttgta caaacaaatg catatatttg tgcggttatt gggcagtgta tgtttttacg
    43981 tgatcttgtt cgtttttcat taccgtacca gtggaaacaa gatcatcagg aagtgatttg
    44041 gattcgtata gtacgaatat gtaatatgtg tgcacaccct tataaaaatc gattaaatct
    44101 ttttcgatta tgttttttta ctaaatgtga tattcctgta ttagttgctt tttgtcaaaa
    44161 gtttttagca caggaaaagt aaaaacagaa aggaggaatg tgacaattga aagctcattt
    44221 tttcaagtat cgtggtcggc gaattcgcta tttcaatcgt tatttatggt tttgctatta
    44281 tggtttcttt ttgtttccat ttttgattct gttaggttgg tttagttgga aagtatttta
    44341 tccacgttta agtacgtttt cgaattggca aatgtatctt attcctggaa taagtattgt
    44401 gttgtatttg ttgctggtta gtgggttaat tttagggttg atgcattggt cgcgactaaa
    44461 agaaggatat tttgcctctg tttattggcg tcagttactt gttcgaatgt taattgacaa
    44521 tcggtttgtt tatacaaaga aacaagttag tgagaaacaa acaagggaaa aaatgagatt
    44581 accaaaagtt tatttttatc aaaagaaaca agaattagtg gtttcttttc ctttagatgg
    44641 tgggcgtttt cacgaacgat ttttaaaaat gggtcatttg ttatctgaag tatttttgcg
    44701 tgatttaatt cgtgaagaac gagaaaaagc ccgaatcaat tatgtattcc tttcagattc
    44761 tggtcggatt cctattgatc aatgtatagc agaaaatgga cgtattcatt taatgaaaac
    44821 tttggattgg gtatatgatc aatcaccaaa tatgttgatt gctggtgcaa tcggtggtgg
    44881 gaaaacctat ttattgtatt ctttaattca agcttgttta agtgttggga cagtagatat
    44941 ttgcgatgga aaagcggcag atttagcagc attaggtgac gtcgatgtat tcaaaggaca
    45001 tgtgttttac aaaataaatg aagaaatgat tatttgttta cgaaatgcat taaaagaaat
    45061 gaatcaacgg tatgcctata tgaaaaccat gaaagatcct aaatatgaac cagggaaaaa
    45121 ttatgcttat tatgggttac caccgcattt tatctttttt gatgaatggg cagcttttta
    45181 tggttcgttg gatatggaag cgagaaatag agtcgataaa ttgattcagc cattagtatt
    45241 aaagggtcgt caagcaggga tgtacattat tttagcaatg caacgaccag atgcagaata
    45301 ttttccaagt ggtgttcggg ataatttaac attccgtatt tctgttggtc gattgtctcc
    45361 cattggttat ttaatgactt ttggagaaga ttttaaaaat aagccatttt acaacaaaga
    45421 tcaacctggt cgtggatatg cttttccagg atttgatgta cctgtagagt tttttgcgcc
    45481 atttgtttac aaagattatc actttatcaa agaatttgag aaatttactg aaatgattgt
    45541 ctatgatttt acagtaaaag aggatagtca agatcaaacg atgggagatg ataatcagtt
    45601 gttacaagaa caactagagc gccaaatgta tttagcaaaa gatccaaaag gaacaagttt
    45661 ttctgatgcg catattttgt aatcaactaa ggaggtgaag ttaaatggca gagagttgtt
    45721 attatagagt agctacagca atacgaatga ttaatccttc tctttcttcc agaacgtttt
    45781 atgattggtt gaatcgtatt gaacaagtaa ctgattaccg ctttttgcgc aaagaacgtg
    45841 tttttacagg aaaagttatt aatcaagtat tgctaacgaa aaaagatata gaacgattga
    45901 ctcgattgta tcattatcga gtagatttag aagaggattt aactttgtca atttatcgcg
    45961 ttttttctcc tgaaaaatat agtgaaataa caaaattaga tcatttaatt ttgtagttat
    46021 tatctatcgg gagggctcta gacgtagtct ggtagccgca ctctgcctgg cggtgcgcat
    46081 taaggctgcg aagcatgggc ctataatgcg cactcgctag cagcgtgtgc agctcacaaa
    46141 gaggtcgtgc gcttgcgcac ggcctctttg tgtaaagccc cccgatatat aatatggggg
    46201 gcagaaataa cataacacat agaaaaaagt tttataaacc cttttgtatc aagtgattat
    46261 ctaaggtcag cagttgtact acttttccca aaaaacgagg gggactagtt gatatatcgg
    46321 tgtttctaaa gggttaaaaa aaatcgattt tgttatttta taaaaggagc gagaaaatga
    46381 caccgattga gctaaaaagc tacagaaaga agttaagtgc aattcataat ataagtggag
    46441 ataaaaagcc gctatcacaa gcaagatttg cgcaattgat gggctgctca aaagattaca
    46501 ttagaaagat ggaacaaggg aaaagaaaaa tcccagagga ttttgaacaa cgaatcaagg
    46561 aacgctttcg attgtatatg aaaagcggaa tagatcttcg agcaatgatt gattatttgc
    46621 gtctttcctt ttttgggaca gactacaaaa cagtgattca acgtgtatta cgaatggatc
    46681 tgacagaatt tgaaacacgt ccaacgggct tgtatctttt tgactgtctt tctgttcgtg
    46741 ggaatatctg ggtgttctgg cattcaaaac gaactgtaac agaaaatgta cttgtgcaat
    46801 tttcaggaca aggttgccgg gaatatgagg aagtcatgcg tgaacaacaa acagattggc
    46861 gaacatggct atattccatt tggaaaaatc aaggaaagtt ggaagctggt tatacacgtg
    46921 tgcaatgtag ccgattcgat attgcgttag atgaactttg gggattggaa acggaagaac
    46981 atttcgatct ctttgaatta ctagaaaaac aaaaagcagg tttattagaa atggacttta
    47041 agagattcca gaatattggc ggttgttttt tagatgatgg ctattttcga agtgaaggat
    47101 tatctttgta ttttggatcg cgaaagagtc ctctcttttt taatttttat gagaaacgtt
    47161 ttgaaattgc gaaccgagaa aaaatttcag aattagaagc attaacgaaa tatggtattt
    47221 ataatcgata tgagttacgg ttagccaatg agaaagcaac acaagcagta gaagcattta
    47281 ttttaggtga ttcaccgaaa cgtttaggag agattggcgt tggtttaatt aatgcaaaac
    47341 ttattgtttg ggatagactg gaaagtgatg cacggattgt tgataaacga tgggcaaaat
    47401 tatttcgttc gatggaagaa ttggatttta cagtaaagcc agaaaaattc tcattagatc
    47461 gttcagaacg ctggtttttg aaacaagtag ctccgacatt aaagttgtta caagaagcag
    47521 atcgtatttt aggaagagac tttgtacaag aagggattga taatgccgaa ctaaatgacg
    47581 agcaacaaaa ctatctagaa tggatgggaa cgtcaggagg gaaaattgca tgaatgcata
    47641 tacgacaaca gaatggctgc gcctgttgga tttaaaggct gcaatcgaag cattaaatga
    47701 aaagatggtt gatttgtctt attttcgttt tcgagttcct tatattgaac aagcggtaaa
    47761 agctggacgt tatcaggaaa aagagaactg gcaagaaatt gcacgcttat tagaagttcg
    47821 taagggatac gaacaagaac tcgaagagtt ggaatttagc cgaaggaaag gaaggctaga
    47881 attcattcgc ttttatcgat tttcattgcc aatacctgcg attttagcgg ttaaaaaagg
    47941 ctgcgataaa atgaagatat atgaaaactg tgtggcagca cttagcagcg aaaaacctct
    48001 tatggaagaa atttcattgg tgactgtcac ttgggaaatg agtcgccagc caacagaaga
    48061 acagacatat ctttctttag aagagattga gatagaatta gaggaaattg gtcgctatgc
    48121 cacttgttct acctattgtg gatcggtcat tagtattgct ggtgtgattg tctaaatgaa
    48181 aaaaattatg atttatgttg gtgcgtattg gtcacgtgat ccaacagtat tagaaaatcc
    48241 agaggcggta cactattgct tgcgccaatt attctatctg tataaagaac gattagaatc
    48301 gttgattcgt cagttaccat atactgacag acgtttagat gaacttcttc taaggtatcc
    48361 agcaatgtat aaacgacgaa aaaatagatt gttaccagaa gagtatccca ttgaaaaaca
    48421 tgaacttgaa gggcgatttg tggcttattt ctatgatgat gttcgtatgc gcttagtaga
    48481 acaacgaatg gaaatagaaa atgatcgcta ctattttatc aattattgta agaagcgtaa
    48541 gtatcaagta actgatgatt tttatgattg tattttacaa gatggggaag tgattctatc
    48601 caaaattgct ccaagttttc gtgaacttgt gccgattgat tttttgaaaa aatgtcattt
    48661 gcggattctt ccgtaaaaaa gagctataaa aggaaagaaa agtagaagcc aaatcggttt
    48721 ctgctttttt atttttttta ggaaggagaa gatggtatgt cactggaatt tttaggagag
    48781 ccaattgaaa aggaagagtt cgtttcgcat tatatggatt tgtttattca ggtgataaaa
    48841 aactttgtgc gattgatgaa ttttatccac gcgaacaaga ctatctagcc gctgaacgaa
    48901 taaaaatcag tttattatgg gaaaaattca cagatgaaaa taaccgaccg ccagattatc
    48961 gctatttatc agattgttta acgaataatg tgctttctaa agaataccta tttcacaaac
    49021 gtgagaaaaa tcaaatgtcg gttgaacatt tttgtgattg ttatattgca ttattgcgta
    49081 gtcaacagtt attaacagta gaacgatatg gtgcagagtt tggttatgtc ttgcaagctg
    49141 agcgtcatag agctacaaga tgctatgaaa aagaagatca agtgattgat ggctatgaag
    49201 aattatgtat tcgaaacaat cgctatttgc aacaacgttt atatgaacaa ttgatgatgg
    49261 atttttcaaa tctctataga gggtatgaat gttcgatgat tcaagaaagg tagaagaaaa
    49321 gggggaattt ttatgtggcg attattattt ttggtggtat gtggtatggc attgttctat
    49381 gtgtatcgtg tctatctcta tccacgaatg gaacgatgga caagttggta tagagatcct
    49441 ttaaaacaac gaaaaggatt gaagcggtat cgttatgaat tacttgttga attttcggat
    49501 gggcgacaag cacagtatta tttttttgca aactatccaa aatacaacac cagtgaacta
    49561 ttgattcaat tattcaaaaa taatccatat gttgggttag aagaaaacgg acgggagttc
    49621 ttttatcgtt cagataatat tagtgcggcg tctatgaaca aaacacgaat tgcataaaaa
    49681 attggaggaa taatcatgaa aaaaacaaca ttagcagtcg taacaacact aggaatttca
    49741 ttattttcgt ttcaattagg gagttcattg gtttatgcag atgaaactgt agaaacacct
    49801 aagtcagaaa ttgtagaaac attgccaggg gaaggattac cagatgttga gacagagcta
    49861 aatggaggaa caacggaaaa accagcagaa cctgaacaat tgcagtcaga gaaacctgaa
    49921 aaagagccag tgacaccaga accagagttt cctgaatcaa ctttaccgac tgattcgatc
    49981 gaagaagaag gtgtgcatag ttcaacaaca acggattcaa gtatagtcac aactccggaa
    50041 caatcaggaa caaccagttc ttctgaaaag aatactgatt cgtcaagtac ggaacagcca
    50101 acgatgtcgt ctaccagtga agaacaaaaa gaagaaccga aacaacctgt tgaaaaatca
    50161 gaagcaattc caaaagaaaa taaacctatt gttccagcaa aagaagtcac agtatcggta
    50221 accccaagcg gtgaaattac gacgaatact tctcaaggaa tgagtgtacc gattgtcact
    50281 agtaatgtag aggaattgac ccatattcca acaccaacca ccccattaaa agtagcaggt
    50341 gatcaaacaa ttgttggtgt aaaagatggt attccattaa tccaagatag tgaagggaat
    50401 ttggtaaagg atttgtcgat tcctgtaaag aaacttccaa gtggcaatat tgaaatcaaa
    50461 acagcagatg gaaaaacaaa agtcttacca aagactggtg aagaaataca tgttgcactt
    50521 tctgttctag gaggagtgtt gacaagtatt gctggatttg ttggatataa aagaaagaga
    50581 aaaggaatag aaaatgaggg taattgaatg gactattttt cagaaaaaca agcttatcgt
    50641 tgtcaatcgt acgtactagt aaaaacatac tagaaggagt agcttatgaa aaaacgacaa
    50701 ctacggcgga aagaaaaagt ttatacagag aaagtaccga agcaaaagaa aatccattca
    50761 aaaaaagcag tgaaatggat ttggtgtttg gttttatttc tttgtttttc agggggcttg
    50821 gcattttttc aatccatgaa aacgaaggtt caagtgtcgt ttttgaaaga tgaaatccaa
    50881 caattacaaa agaaagaagt acaaagtcgg tcagaattaa cgtacacacc tgaaattgaa
    50941 tcctttatga atcgttttgt tgctttgtat atgaatacat tcgataatca tgaacaagaa
    51001 caacaacgtc aagaaacatt aaaaaaagag ttttatgcac aaaactttat tcaagaacca
    51061 ggaagtgttt caggtgttcg aaaattaact gctgcttcgt ttattggatt aaaagaaatt
    51121 gaaaccattc caactgctag ttataaagta acttatgata ttcgctcgat gaatccagag
    51181 agtaagaaag aaacaagtgt gacaatcaca caagtactca atcttccctt ttctatttca
    51241 aacaagggct gtcgtattat tgcttatcct tattttacgg cactaccaaa gaatacagaa
    51301 aaacaacgaa tgattactta tgatgacaca ttatacgaaa cggttgatgc gtcggaagcg
    51361 aaagatgttg aaggatttgt ggaagatttc ttagataagt atgcgaaaaa caatgcaaaa
    51421 gatatgtcat acatgatgaa agaacctgaa ggactcaatc aaacagctaa agttgatcaa
    51481 gtagagatga aagtattgcg ttataaagaa acgaaaaaac cttttattgt aaaagcaacg
    51541 gtcgtctttc gagatggtga tacgtcactt ttacaaaaag aacaaatgac gattgtcatt
    51601 acgaaaaaaa caaatcagta ttatgttgaa aaattaacac ataattggtt ggataggaaa
    51661 ggagaagaac ggaaatgaca ctgaagggaa ttgtagattt tatattaacg caaggtggct
    51721 attttttaat aatggtggtt gtttatctgg gattgcgaaa ttttatgaaa tcaaaagtag
    51781 gaaatgcatt aatatctatc gcagcaggtg ccgcagttta tttcttctta aatgatccaa
    51841 cacgtgtatt tgatgcgttt ggtaaaatca ttgaaaaatt atttatggat taggaggata
    51901 gtgaagtgga ttgggaacct tatgattatt cgattgttta tgcggaacct tatcgcgtac
    51961 aacagattac aagcaattgg cgtcttccat ttccagttgc attagtcaat gcagcaactt
    52021 ttttgttggt cgtaatcttt gaatatttat ttttacgttc atttattggt tgggtcattt
    52081 ctttttttcc accagcagga gttgtttttt atataggact cccctattta tgtgtgttag
    52141 cactggcaaa actaaaacct gatggaaaaa aagtgcattt ttatttatgg gattggtttt
    52201 gttatatgat tcgtatgatg aatggtgatt gtgtgtatta taactgggac aaggtatcaa
    52261 acaatgcatt ggaagaatta ctttttgtga ataagtatgt ttatttagag gattagaaaa
    52321 agggacaatc aactggttgt tcctttttta ttgggaggtg aaattcattg gtgcttgtaa
    52381 aagaaccagt aaaagcattc aagaaaaatt gtttgttaaa tggagaggat tgtatctggg
    52441 cctactatca tgtttttcca cgaacaattt caaaaaacac accaaaaaag acacaggaaa
    52501 taaaagaaat gatggctcgt ttactgttgc ttttagcaga ttatgaagat gtggagttgg
    52561 tgatgtatcc acgaacaatg aatttacgga cgaagtttga cacattagga aagggatttt
    52621 ctcccgatag tcaagcaatt ggtgcgtatt atgcaaaaga aacagtgaca ttgttggaac
    52681 gagaaggtca agtctatcgt ccttcattta ttttaggaat caaagtcaaa gaaaaagcaa
    52741 cggtggagag ttttcatgat gtaataaatc taatctggtt agatttagca ggaagtttag
    52801 cacgcttatt tggattagaa gtagatgatt tggaaacttt gttttctgaa agtgatcctt
    52861 atgtaaaaga agtgacacaa ttactttata gcttttctat tcgtccctta gtaaaaaaag
    52921 agctgttgta tctcaatcgt ctgcaatata ttcgaggaat ggctcacaaa gtagaagaac
    52981 aaaataattg ggaagtactg aaagacaatc aaggaacgta tgtacgtata ccgaaggaac
    53041 ataaaggcct tcttcaatta gaagatgagt tagggagtag ttatttagca ttgttgccta
    53101 ttggggaaac acctgaaaat atgagaaatt gtgatctatt tcaatttgct caggatcttt
    53161 ctttcccagc agaattgcga atgaaaattc actatccacg cttgaaagga cagggaggta
    53221 ttggtttaaa actgaattgg ctatcggaac ggttcaaaac agagcataag gaattggttc
    53281 aaaatgatga agaacctagt gaacgcttat taactgttcg aggattagta aaacatttaa
    53341 agcaacgagt agataatggg caacctgtcg tcgattggat cgcatgtttt attgtatacg
    53401 gtcaaacggt tgaagaagtt cgtcaaaata gtgcaacggt tattcaacaa cttcatacga
    53461 ttatccatat ccaccgtgca aaaaatgatc aacgagaatt gttctataaa tgtttattag
    53521 ggcaaccatt gggacggcag ttacattgga agcaccgaac aactgtagaa gcagttggcg
    53581 aatggttgtt ttcagcagta tcagaattag gtatggaatc cggctggtat attggtcggc
    53641 aagatacatt aggggatggt ggttctcgag gagaaaaaac accattagaa gaattaattt
    53701 atgcgtcaaa tcgttttgtc tttttgaatc ttctagccat tgcggaagga atcaaaggtg
    53761 cgttgtttga tgcgcctcat gtagcagtaa ctggaaaaac cggtaaaggg aagacctttt
    53821 tagttggctt gctctttttg tatagttcgt ttatgaatgt acaaagttta tttgtggatc
    53881 ccaaaggaga aaagcgcaaa tggtttacga aactcattca agacccgtat tatcagaaac
    53941 attacccttt gtttgtagaa catttgcaaa aaatgagtta tgtcacgtta gatgctacaa
    54001 atccccgtaa ttatggtgta ttagatccaa ttgtttgttt atcccgagta gatgcgaaac
    54061 aagtagccca ggatatgatt gatgagttaa gtccgttagg taccaatcac cttttaaaag
    54121 gggcggtatt aaagggaatc caagaagttt tagttcaaaa agaagccgga caacaagttg
    54181 gcttgatgca tgtggtcgac tatttagaac aaatggaaca aaaagaggtt cgagaatatg
    54241 gtgagttttt acgcttgacg gtagaagatt cgattttacg tttaggattt tcttatggag
    54301 aaaacccggg attagacttt aatgccaaaa caacgatttt agagatccaa gatttgaaat
    54361 taccaaatga caatgtccga ccagaactat atacagatgc ggatcgaaag tctttgtgtt
    54421 tgatgattag tttaggtcgt ttttgtgaaa tgtttggaaa acgagatagt agtaagaaaa
    54481 cagcgattta ttttacagaa gcatgggtat ttaataattc gaacgcagga cgtagtatta
    54541 ttgcagcaat ggcacgtgtg gggcgttccc agatgaatca attagtgtta gatacacaat
    54601 ttattggtga cttaggttca gataaagaaa aagggaattt tggtgtcgtc tttgcttttg
    54661 atgaagacag tgagcgagaa aagattttga atcatttacg tttggaagta aatgaagcga
    54721 acttagctga aatgagccac atgattaaag gacagtgttg gatgttagat ccctatggac
    54781 gtgtaggaaa actcaatgta cacagtttgt ttgaagaaat gaccgctgca ttacaaacaa
    54841 cggaaaaaac aagtcactct caagtagaag aacaggatac gttaatcatg caatagaaat
    54901 gaggtgaaac aatggcttat ataaagcata aggaaaagaa atggtgcgtt ttgttttttt
    54961 taacgattgg attcggactg ctttttgttg gtggattggt gtatgcggca gaaaagcagg
    55021 tggatcaatc gatctatgag attgggaaat ttcatattga taccttttct tcttatttat
    55081 ctgatagtga attttcatta ggcaaaatgt tatttggggc aaatggcaca gattttctaa
    55141 aaatgatgac gaatatgttc tttagtttaa gtaaattgat ttggcaaggc cttgattttg
    55201 tcattgagaa gctctatgaa ggagctgcaa tgaacgagtt aattcaccag tttttcactt
    55261 tttcggatac tatttattca aaaatatacg atcctattgg tgcggggatt gtcgtcttgt
    55321 atgtgttata tctatttact cttcgtatat ttagagggaa acattacgca aaaatggtgt
    55381 ttattcgatt tctcttcgtt gtcatttttt caatggtttg gtttggctat ggtagtaacg
    55441 tacctagtaa aggagaagct tttaccaaag ggttaaatga tttgtccgtg gaggtagaag
    55501 gctttatttt ttctgctaca aatagtctag atgaattggc gacaaaagat gtcaatggtg
    55561 ttttagtgcc aacgacacaa aaggaagcca ttcaaaaagt gcgccaactt tattataaag
    55621 cggctgttgt tgatccgtat atattgttaa attatgggac aacaaattta gaagaattgt
    55681 atcaagcaga gattgaacca caagaatttc tagccaagga agcgtcaagt gaagaattat
    55741 ctgatattga gacaaaagtg accactgctg caggagaaga tagagggaaa aagaaggctt
    55801 atcgtgtcta tattcaacca actaaagctg tttataaagc agtcgttggt tctatggtgc
    55861 cattgcttaa tctaagtatt ggattaccac ttgtagtgat tggcatgatt cgttttatct
    55921 ttcaaatgtt ggtgttgtgt ttattacttg tgattccttt ttcattactc gcttcttttg
    55981 ttccagtatt tgattatatg ctcatgaata ccttaaagtc ctttttgggc ttgatgtttc
    56041 aaaaatcttt ttacagtgtg attattttgg tggcattttt gatctataac atagtagacg
    56101 atttaattcc aatgaatact gtttttggtt ttttaggaaa tatgtttgtc aaagcactcc
    56161 tatcaatttt actcattgta aaacggaaag atttattaaa gaaactaggg ttaggagctg
    56221 cagatcaaac tataaacaac acgaaaaaca tagcaagtca ttctttgtct caaatgaaac
    56281 aactgcctgg tcaactatca actgctggac aaaatttggc aatcaaaggt gcaggattag
    56341 cagggaatgt gtatcgagca acgaatccac tcgcttctgc agcgatacat ggatttcaaa
    56401 agtttcaaca aaaacgtaca ccacaagaat cggtaaaagg gaggcaagtg gttaatccct
    56461 ttgaactagg tgggcgttca aatcaagcct tgccttatag tggtcgtcat gttcgttatc
    56521 atggggtaga gcctgaaatg tcttctacca aatcgaaaac agagtggaat tctggtcttt
    56581 caaatggttt gattccaaat tcgtccatga attcagtaag accaaaacaa caggtacaat
    56641 cattggcaca attgagtaag caacaaggga ataatcaaag aaaaccattg ttaaataaag
    56701 gtatcgcttt tgttggtcaa tcgattccgt tgccttctgg aaaagtaccc gtgccacaat
    56761 taacagctgc tggttctctt tcacaacaga ttcgccaagt gaaaaatcag gcgaaaaatg
    56821 ggagttatcc agcaatgcag cagttattga accaatactc tgttcctaat tcgtcggtag
    56881 ccactctatc aggaaatcgt tctgtgcaac aaccggggca acgttcgtct gaaagtaaac
    56941 cgtcgatttc cgcaccaaaa tcgttacaac ctgcattttc ttctttagat aagccaacag
    57001 agcaatcgga aaaaatagaa aaggatccta aaacactgcc gattttggac gcaaaaccag
    57061 gaactgattt tactttacca aaaggacgaa cagtccaaaa accattatcg aagaaaggat
    57121 gaggaagaaa tgaagaaagg gtgtctaggt tgtcttcttt tctttattct tccctttttt
    57181 ctattgccat tctttttttc aggagaactt tctgagcatg gaggagaagc tacaggtcta
    57241 gctgtcgctt ttaatgggga atataccgaa ggattaccga ttttttcaga gattaaagga
    57301 cgtggtccaa tcacagatga acatgcgcaa gcagcagttg gtgcagctgt aaagtatcag
    57361 ttattaccga gtgtgattct ttctcaatta ggctatgaat cagcttatgg acagtcacaa
    57421 tctggaagaa acgatacgaa cttttttggg attacctggt ttcctggttg tccctaccca
    57481 caaggttctg cacgtggaat tggtggttct gaaggtggtt tctatatgaa gtttccgaac
    57541 ttaaaagctt gttatgacta ttatggcttt atggtggcga gccaaagcaa ctttaatcaa
    57601 tgtgtgggca ataaagatcc tggaagttgt ttattgattc taggtcgtgg aggctatgcg
    57661 gcagcaggta ttaccgaagg gagtccgtat tatcgaggag caatgagtat cataacgtcc
    57721 tataacttaa cggaatatga tacttttgcg atcaagcatt ggaatagcct accaacaaaa
    57781 aaaacttcgc ttattgcagg aaaaagtaaa ggcaatgcga aggttttgga tcggatcatt
    57841 ggacgatccg taaataatgg ggaatgttat ggtggtacgg catggtatgt atctcaatta
    57901 ggtggcccac agttaatggg ttctggacac atgtttgcga tgttaatagg ggaagattat
    57961 aactggacaa cctatggttg gcaagtaatc atgaacccaa aaccaagtga tttacaagca
    58021 ggagatgtgg tgaattggca agcaggtggt gcattgtctc ctggaatcta tggtcacacc
    58081 ggaattataa cagatgtttc atatggcgga caacatttct ctagtgtgga acagaatgcc
    58141 gagcaaggac ggattatggc tcggtatcaa cgcacgtaca atcaaacaaa aattcgtagt
    58201 ctggttcgta aggtgaaata agaaaggcag tgactagaaa cgtgaaaaaa gggataattt
    58261 tagtatttag ttggttatgt ttgataggtc ttactggttg tcattggttt tcttctgaaa
    58321 aacaaaaaac acaattgaga acgacagaag aaacaatcga agcagtaaaa gaaacaagtg
    58381 gaactcttca aagtactgaa agtaacagtc aaattggttc attagaagac gagacagcgc
    58441 aacaaatgca aatagatgaa atgacaaaaa atttcttaaa agattttgct agaaaatgga
    58501 gtaatttcac agatgtttat aaacgcaatc aagctgtgag ggactatatg acagatcgtt
    58561 gtattaaaga caatagtatt gatgtagacc cgcatgtaca aatgaaaaca actgggaaac
    58621 tttaccaggt gacacaagat atagagaatc cggatcaata tttgttattt ggagaagaaa
    58681 caatgaataa ggttactcaa tttgtattac ttcaattgaa agtagatcaa gaagctcaaa
    58741 agattgatgc gatcaagatt tattatgtac ggagtgcgta ttaaaataaa aaatgaacac
    58801 tcatatttat tatacaccga agaaatagaa aaaaacagac tgttttttta agaaaaacta
    58861 tgtcattctt tacttgaaaa aagttgttat aggacagaaa ggataaaaca aaaacggaaa
    58921 aaatatgtct tatgaataac tataagaact attctatttt ttaggagatg gatacgagat
    58981 gaaaaaacta attggctata gtgtgatgtg tattggattt gttggtgtgg gattgtttgt
    59041 atcgactgaa tctgcttctg cggctgaatg gtataatcct atctggtata atccgaacat
    59101 tgaatgtcaa aaaggctatc cgtatggacc gacaaatctg aaaaatggaa aatgtagagt
    59161 aaattggaat ggcgttgtag atagtgtaat tactggttca atggactctg ctctacatgg
    59221 ctttacgggt cactagaagg aggaagaaaa tgtctgaaga gaaaaaagaa atcgttgaat
    59281 cattagtagc tttaaaagat tctctttcta agattgaata tgttgatagg caagaaattc
    59341 aaaaactaat tgatgatact attattgaaa tccaagatgc gcgttgtgaa ggtataaaaa
    59401 tttctgtagc tttatctaaa gtaatagaaa aaatgaaccg ttcattagcg tttaatggat
    59461 tgaagctaga tagacaaact agtctgatat gggatcattt aaaagacttg tatgacaagt
    59521 ctaaaagaag tgaacggact gctgtatcaa tcttaaaagg cctttgggga atgaattcat
    59581 aaacaagata tttaaaaagt aacttatttt ttatggagga gaaattatga aaagaataat
    59641 taaaatattt ggtgttttag gtgttttctt attaggttta agtacttttg ttagtactgg
    59701 aacaagtgtg tatgccacag aaaaagtgaa tacagttgat ttagaaataa agaataaatt
    59761 agtagatgaa ttaacttttt attttgatac agttggtttt ttagacgaac atggtgtata
    59821 ccatataaag gattttggtg ctattcgtga acgaatcagt caagataacg aacaaggaag
    59881 agcagcaaga gtcttttatg aacatattat tgttcctaga atggagaaag ttgattggaa
    59941 acgatatgct ggatgtgtag ccattaattc tcttcctgga ggaggagtgg cttgggctat
    60001 aggtacgcag ggagatttta ttgaagcatt aaaagctttt aattttgaaa aagctgcgga
    60061 tttattatta aaaattgcag aaaaacaatt aggaaaagct gcagctaaag agttagctaa
    60121 ggtaaatatt gtagcaggaa ttgcagcagc tgctgctagc tgtgcattag agtaaaagtt
    60181 tatgaaaagt ttagaacaag agcatatggc ttttaaacaa gcaatgttta aagaaaatat
    60241 ctatttaaac cataactata ttcgtgtttc taaggcatgt tcaccagtat tgaatatgct
    60301 tggaggtgga aatggattat atcatttact gtttgtcgat gtttgttggt taattttttt
    60361 accagatgaa ctagttatag tcaatgaaaa aataacaagt aaaaatgaag tgtttaatta
    60421 ttctttaaca agaattaatt acaaagagat taccaagttt tcactagaaa aagttccttt
    60481 ttggggagaa ttttgtttaa aaattaaatg taactggaaa agaatgtatt tctatattga
    60541 tggtgatgat gctcttacat tcggtaaaac tactttttct agttttaatt ttcagtttct
    60601 attaaaaaat aatttttatg gtttattgaa ataaggttgc gaaggaagat gtagagttat
    60661 ttgcactgtt gtgttggtat atctcttaat gctgtgtctt tttggggaga ttaattagtg
    60721 gagtgattat atgagtatag atgattgttt agaagaacaa ataagattat ttttaaagtt
    60781 tttaaaagtt aaagttcaaa ataactactt cacatgttat agttatgcac accctgtttt
    60841 aaatagtctt ccttttaatt ctttagtgca aggattgatt acaaatattg ttttattagt
    60901 ctgtacacca aatgaaatat ttgtaaaaaa gataggcagt ggtatttctt cagtaagatt
    60961 aaaagagaaa gaatatactc aaaagattat tcatattcct aagcctgaaa ttaagcgttt
    61021 tgagataaaa gactattctg ctttagggct gttctcaatt ggttactggt gataaaaaat
    61081 aagaatacta aaactatacg tagaatataa gtaatcggca tcaatcctaa ttatgtgatt
    61141 gaatgccgat ttttgttacg cgctattttt aattatctgt aagaagaata attttatttt
    61201 actcatattc gttttcactt tgaagaagta aattgcaatt tcttataagt taagcagcta
    61261 tgcaaaaggc tatttattct attgtgaaat ataagatgta gttgaaacag gatatataga
    61321 atcaaaatac gaatcttatt ttgatttttc tagttttttg aaaaaagcac cctttgatcg
    61381 ttgtattagt ggaaaagaga aaaaggagca tacttatcat gtcacaaaac tacaactaaa
    61441 tatctaaaca acaccaattt gatgcctatt gaaaaaaagt attgcgaaat gaagccaagt
    61501 ctattagaaa atgaaataat agaataaaag aaaatgggaa accgctgaat gatgtaaatg
    61561 aaggtaagta tccacgaact aattttgatg aacaagatat ctatttttta tttgtattgg
    61621 agatactagt ttaatatcaa aagttatcag cagtcataat tagttatcca atgtaaaaag
    61681 tacaaacaat agttgttatg cagaatagtt tatgaaatat attacataat aagaaaacaa
    61741 gcgatatttt tctgatcaga acaaccattt aatttaaaaa gagttaaaag ttatatattt
    61801 ttgaaatatt ttggatgagt agctaaaaca atattttgct cattaagcat taaatgaaga
    61861 tgcaacgatg ttagaaagtt agcttcttct ggatcttgtt ttgttaatgc ctcaaatagt
    61921 tgataatgtt gttccataat tttttcccac tttaatcctt ccgtttttac tcgtagccat
    61981 cgaaatcgtt ctaagtgtgt attgttattt tctaaccatt gccatacttc ttctcgctct
    62041 gcaatttcaa aacaagcttt atggaataaa ttatcattat gaaaaaaact acgagcattt
    62101 ttttgactaa cagctttttc ttgttctgaa attatagttt ctaaaatccg ttttttttgg
    62161 ttattcattt tggcacaaca ctctaacata atttgtcgtt ccaaatgttc tctgacaaat
    62221 cgagcatttt tggccgaatc taaatcaatt aatgagatat aatttccgct ttgaggaatt
    62281 gtatcaacaa gaccttgttg tcggagttga atcagtgctt cacgtattgg tgtacgacca
    62341 atagaaagtg tttcttctaa ttttttttct gatacttttt ttccaggttc taagtcagca
    62401 tagataattt gtcggcgaat agattggtaa gcttgatttt ggagactagt tttaattgtc
    62461 atacacaatg ctccttaact tttattttaa ctgatatttt aat
//
LOCUS       DACCXO010000004        61759 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.004, whole genome shotgun sequence.
ACCESSION   DACCXO010000004 DACCXO010000000
VERSION     DACCXO010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.004"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(57..1829)
                     /locus_tag="IVY70_000201"
     CDS             complement(57..1829)
                     /locus_tag="IVY70_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7957364.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1783..3537)
                     /locus_tag="IVY70_000202"
     CDS             complement(1783..3537)
                     /locus_tag="IVY70_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7957365.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3647..4216)
                     /locus_tag="IVY70_000203"
     CDS             complement(3647..4216)
                     /locus_tag="IVY70_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP7957366.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAIIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4234..5658)
                     /locus_tag="IVY70_000204"
     CDS             complement(4234..5658)
                     /locus_tag="IVY70_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7957367.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(5660..6328)
                     /locus_tag="IVY70_000205"
     CDS             complement(5660..6328)
                     /locus_tag="IVY70_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP7957368.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(6458..7042)
                     /locus_tag="IVY70_000206"
     CDS             complement(6458..7042)
                     /locus_tag="IVY70_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7957369.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(7373..7606)
                     /locus_tag="IVY70_000207"
     CDS             complement(7373..7606)
                     /locus_tag="IVY70_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP7957370.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(7621..8571)
                     /locus_tag="IVY70_000208"
     CDS             complement(7621..8571)
                     /locus_tag="IVY70_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP7957371.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(8568..9410)
                     /locus_tag="IVY70_000209"
     CDS             complement(8568..9410)
                     /locus_tag="IVY70_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP7957372.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(9412..10131)
                     /locus_tag="IVY70_000210"
     CDS             complement(9412..10131)
                     /locus_tag="IVY70_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP7957373.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            10823..10981
                     /locus_tag="IVY70_000211"
     CDS             10823..10981
                     /locus_tag="IVY70_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7957374.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            11053..12279
                     /locus_tag="IVY70_000212"
     CDS             11053..12279
                     /locus_tag="IVY70_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7957375.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(12367..12759)
                     /gene="rpsI"
                     /locus_tag="IVY70_000213"
     CDS             complement(12367..12759)
                     /gene="rpsI"
                     /locus_tag="IVY70_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP7957376.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(12775..13218)
                     /gene="rplM"
                     /locus_tag="IVY70_000214"
     CDS             complement(12775..13218)
                     /gene="rplM"
                     /locus_tag="IVY70_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP7957377.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(13737..14204)
                     /locus_tag="IVY70_000215"
     CDS             complement(13737..14204)
                     /locus_tag="IVY70_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP7957378.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(14324..15862)
                     /locus_tag="IVY70_000216"
     CDS             complement(14324..15862)
                     /locus_tag="IVY70_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP7957379.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            15942..16628
                     /locus_tag="IVY70_000217"
     CDS             15942..16628
                     /locus_tag="IVY70_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP7957380.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(16829..17356)
                     /gene="msrA"
                     /locus_tag="IVY70_000218"
     CDS             complement(16829..17356)
                     /gene="msrA"
                     /locus_tag="IVY70_000218"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7957381.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(17440..18117)
                     /gene="pgmB"
                     /locus_tag="IVY70_000219"
     CDS             complement(17440..18117)
                     /gene="pgmB"
                     /locus_tag="IVY70_000219"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP7957382.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(18114..19244)
                     /locus_tag="IVY70_000220"
     CDS             complement(18114..19244)
                     /locus_tag="IVY70_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7957383.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(19244..21427)
                     /locus_tag="IVY70_000221"
     CDS             complement(19244..21427)
                     /locus_tag="IVY70_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP7957384.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(21424..22449)
                     /locus_tag="IVY70_000222"
     CDS             complement(21424..22449)
                     /locus_tag="IVY70_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7957385.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(22494..23321)
                     /locus_tag="IVY70_000223"
     CDS             complement(22494..23321)
                     /locus_tag="IVY70_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP7957386.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(23318..24385)
                     /locus_tag="IVY70_000224"
     CDS             complement(23318..24385)
                     /locus_tag="IVY70_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP7957387.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(24411..25247)
                     /locus_tag="IVY70_000225"
     CDS             complement(24411..25247)
                     /locus_tag="IVY70_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7957388.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(25244..26113)
                     /locus_tag="IVY70_000226"
     CDS             complement(25244..26113)
                     /locus_tag="IVY70_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7957389.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(26183..27466)
                     /locus_tag="IVY70_000227"
     CDS             complement(26183..27466)
                     /locus_tag="IVY70_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7957390.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(27480..29165)
                     /locus_tag="IVY70_000228"
     CDS             complement(27480..29165)
                     /locus_tag="IVY70_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP7957391.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            29440..30465
                     /locus_tag="IVY70_000229"
     CDS             29440..30465
                     /locus_tag="IVY70_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7957392.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(31060..34713)
                     /gene="rpoC"
                     /locus_tag="IVY70_000230"
     CDS             complement(31060..34713)
                     /gene="rpoC"
                     /locus_tag="IVY70_000230"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP7957393.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(34792..38409)
                     /gene="rpoB"
                     /locus_tag="IVY70_000231"
     CDS             complement(34792..38409)
                     /gene="rpoB"
                     /locus_tag="IVY70_000231"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP7957394.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            39137..40105
                     /locus_tag="IVY70_000232"
     CDS             39137..40105
                     /locus_tag="IVY70_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7957395.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            40123..41028
                     /locus_tag="IVY70_000233"
     CDS             40123..41028
                     /locus_tag="IVY70_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7957396.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            41025..41831
                     /locus_tag="IVY70_000234"
     CDS             41025..41831
                     /locus_tag="IVY70_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7957397.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            41988..42956
                     /locus_tag="IVY70_000235"
     CDS             41988..42956
                     /locus_tag="IVY70_000235"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP7957398.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(43273..43797)
                     /locus_tag="IVY70_000236"
     CDS             complement(43273..43797)
                     /locus_tag="IVY70_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP7957399.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            44084..45895
                     /gene="scm"
                     /locus_tag="IVY70_000237"
     CDS             44084..45895
                     /gene="scm"
                     /locus_tag="IVY70_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP7957400.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(46050..47036)
                     /locus_tag="IVY70_000238"
     CDS             complement(46050..47036)
                     /locus_tag="IVY70_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP7957401.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(47059..47625)
                     /locus_tag="IVY70_000239"
     CDS             complement(47059..47625)
                     /locus_tag="IVY70_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP7957402.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(47639..48055)
                     /locus_tag="IVY70_000240"
     CDS             complement(47639..48055)
                     /locus_tag="IVY70_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP7957403.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(48512..50446)
                     /locus_tag="IVY70_000241"
     CDS             complement(48512..50446)
                     /locus_tag="IVY70_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7957404.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            50748..51683
                     /locus_tag="IVY70_000242"
     CDS             50748..51683
                     /locus_tag="IVY70_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP7957405.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            51773..52876
                     /locus_tag="IVY70_000243"
     CDS             51773..52876
                     /locus_tag="IVY70_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP7957406.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            52889..53842
                     /gene="menA"
                     /locus_tag="IVY70_000244"
     CDS             52889..53842
                     /gene="menA"
                     /locus_tag="IVY70_000244"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP7957407.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(54777..56123)
                     /gene="gor"
                     /locus_tag="IVY70_000245"
     CDS             complement(54777..56123)
                     /gene="gor"
                     /locus_tag="IVY70_000245"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP7957408.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(56257..57498)
                     /locus_tag="IVY70_000246"
     CDS             complement(56257..57498)
                     /locus_tag="IVY70_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP7957409.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(57516..58439)
                     /locus_tag="IVY70_000247"
     CDS             complement(57516..58439)
                     /locus_tag="IVY70_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP7957410.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(58660..61152)
                     /locus_tag="IVY70_000248"
     CDS             complement(58660..61152)
                     /locus_tag="IVY70_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP7957411.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(61159..61626)
                     /locus_tag="IVY70_000249"
     CDS             complement(61159..61626)
                     /locus_tag="IVY70_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP7957412.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT        18367 a        12738 c        10805 g        19849 t
ORIGIN      
        1 gaaaaagaat tataactata tcccaaaaat tgtaggttaa tttaaagcct atttcatcat
       61 cgtgattgag cttctttttc atagacgaat ttctcagata gtttagtttt ctttatgaga
      121 tttttgtata tttctgacct tcgatacaac tcattatgag taccttgatc aactagtttt
      181 ccttgatcca tcactacaat tttatttacc ttttcaatag acttcaagcg atgcgatata
      241 attagtactg tcttatcttg tattaaacga tttaaacttt cctgaatacg tttttcattt
      301 tccgcatcta aactggcagt aatttcatct aaaattaaaa ttggcgcatt tttcaaaaaa
      361 gcccgtgcta tggataggcg ttgtcgctct cctccagaaa gtgaacttcc gttttctccg
      421 accaatgtat gataaccgtc aggtagtttt tcaataaagt cttcgcagtt agctaaacga
      481 gctgcttttt tcacttcctc atctgacgct tgactatttc caatacgtat attttccaaa
      541 atacttgtat taaacaaagt tacctcttga aaaacaatag cgattttgct aaacaaagaa
      601 gctggagata cacgtttaat atcataccca tcaattaaaa tacagccttc atcataatca
      661 tacagtctag acactaattt caagatacta gttttcccgg aaccactagc acctactaac
      721 gcagtcactt ctccttgttt agccgtaaaa gagatatgat ctaaaattgg ggtattgtta
      781 tcatatgaga aagagacatc tcgcaactca atgtcaaaag atttcagagg actatctgat
      841 ccctcttgga tactggtttc cttcatagct ctaattcgct gtattttagg agctaaataa
      901 aatatttcaa gcactgcttc tttcatagaa tcgaaggaat cctttatttt tattgcggct
      961 agtaagtaac caacgacgta gagtatagtc acttcacccg ttaacagtaa gtgaacacct
     1021 actagaatga ctactgctaa actaacatat gagaaaatag aggataaggc catgactgaa
     1081 aaagtactta attctacctt taaatgaatc ctttcactct cttccatttt tttgaaaagt
     1141 ctgtcttgaa cttttttaga tagattaaag ctattaatct cttgctgcaa ttctatagtt
     1201 tcttgaaatt cttccgagtt ttctctcaaa gtatcgtaat atttagtatt ggctttcgtt
     1261 tgggatttct ttgataacaa gattaagaca aaactaaata acgttggcaa aataacagct
     1321 aatcccattt tgacattacc aaccaaaagc atcactgaaa taaaagggaa aaacagagcc
     1381 ataccgccgg ctttaggtat tgcatgactc atcgcatgct caataccttc aacgtcagac
     1441 atgatagttt gagataaatc tgataaatta tgttttgaaa aataggataa tggtaaattt
     1501 gacaaatcgt ctgcaatttg cactcttaaa tgcgcacttt ctttataagt actattatat
     1561 aagttttcat actccctatc taacaaccaa tacatgaaaa gcaaagtaac aaacgatacg
     1621 gcaaaataaa gccaacgggg ctttgtattt tctaaaacta attcgtctat cagcatcatt
     1681 ataatgaatg ctggtaccat attaatgcag tatacaaaaa aactagaaat agaagcttta
     1741 gttaaagctt ctgcgccctg atcacttaaa gcaaatttgt ttttataaaa acttttcatt
     1801 gtcaagcctc caatcatttg cattctcata attagtgacc aattgtttat acacttgatg
     1861 tttagccatt aattcattat gatttccacg ttctactatt ttcccttctg acaagacaag
     1921 aatttcatct acagatttaa tgctggataa tcgatgagct atcataataa ctgttttttc
     1981 tttcatcaga tgttgaaaag ccttttgtag ttcatactca ttatctggat caatagatgc
     2041 actggcttca tccataataa caatctttgc atcttttaaa attgctctgg caatagctat
     2101 acgttgtttt tctcctcctg acagataaac tcctttacta ccaattaagg tatgctctct
     2161 ttcgggaaat ttttctaaaa tcgattcgca accggctaat cttagcgcgt caaatacttt
     2221 acttgctggc gcttgaggat ttgctataga aacgttatcg taaatactct gattgaatag
     2281 tttcgtatct tgaaagacaa aagcaattgt gttaataatt gtgtcttttg aatagtgatt
     2341 taaaggctta ccaccaattt taatctgtcc cttatccaca ttgtagaaac ctgaaattag
     2401 tttagctatc gtactctttc cgcttcctga ttttcctact aatgcataaa ttcttttttg
     2461 ttctaaaact aagttgaaat tttctaaaac acactcttct ttgtatgaaa agctgacatt
     2521 ttcaaatgca atgtcacaat ttttaatctg tgtctcgttc ccatatttta gttggtcttg
     2581 cttcatttct aaataaaggg agtctagtgt ttcgactgca tatcttgctt caaacaagtg
     2641 catacttaca tacattattc tcatgaaaga aacaaaaata attccactta aaaaaaacaa
     2701 catcaacaag tcaacagcta atatcttggg agtggtaaga gaagttataa aaaaggttat
     2761 aggaactatc aaaatgcaaa ctatgccaaa aaatagccat tgaaaaagaa caaaaggtgt
     2821 tttcccactc aaagaatact tatatgcaaa cttagcataa tcgttaattg ctttattcaa
     2881 ggctttaaat gatgttactt tagcaccaaa tatctttata acttgaattc cgcggatata
     2941 ttctaccgtt tcagagctta acacatctaa cgattcttga tatttttgaa taaaatttgt
     3001 attctctgtc attttcttta acaagaatac accaattact aacatggcga ttagaaccaa
     3061 tccaatttta aagttaatga aaaagcctaa ggcaactgat aaaacaggtg ttactatggc
     3121 ttgagcacta tctggaatca agtgagcaat tgccatatgt gttttagccg cgttgtcatc
     3181 aattgtcttt cgaatatatc ccgaagaatt taaatcaaaa aaacgaaaac ttgcttctgt
     3241 taaaccgcta attccctttt ttctcaaatt agtctctaag cgaaacccaa ggatatgaga
     3301 aaataagcca gaaaaaatat aaaataaagc tcctaaagtt aaccaagcaa cagtttgaac
     3361 cgcataactc ttagctaaat attcatctcc tacaataatt agagactgaa gaaatttgta
     3421 aatactgtaa tatccataga ctgttaatac gacagaaaaa tcagaaaata aagtggctaa
     3481 ccatccgtag tatttcattt tagggacata actaaataat tttttgtata ctttcatttt
     3541 ttcctccccc gcattcaatt tcttctctta ttcgtttaca actgttaatg ctttccgtaa
     3601 tagcgtaaat tagagatgta caaacagata aaaggtaaac tacgttctat tttttgtatt
     3661 tttttaagac ttttcttcca atcaatacac caatcataga taaacttgct acaactaaca
     3721 ctgaaatgat tccccatata cctaccgcat agtggataat tgcatccatt gttgcagcag
     3781 attgtccttt atctatccaa tcttgacgaa aacgatctgt aaagaaccat ataggaatcc
     3841 actgtccggc agcactgcct aattgcatta gagcccaaga cgcaacttga ggaattgctc
     3901 gatcatatcc aaatacagat agaataatat cagctaagat cgccatacta atcgaactga
     3961 taaaccaagg taagtacgcc ccgcccatta acgtaaaaaa taacaataaa acaattgtgt
     4021 aaatagtaaa aattcctttt ctagggtagt tatagctaat ataaacaaat atgattccag
     4081 ataataatcc aaaaatcccg ggggatatgc tgtgaccaaa cgccccacca atactactaa
     4141 ccattattga aaaaaagtaa attactaggg aaataactgt agctaaacca ataaaaatat
     4201 aatcttttgt tttcatatat attctcctca attctatttc gttatgtcat taaaaatctg
     4261 ttctatcaaa gttgaatctt ctactctaag ttttatatct ttttttattc caccattttt
     4321 taaataaata attctattgc atgttttata aagaaattcc ggatcatgag tagctataac
     4381 tactgcaccc ttttctttta agtctttaat aaggtcagca atcctttcca tattaatata
     4441 atccaaccca cttgtaggtt catcaaatag aaataaatta gtttttgaca atgaagctat
     4501 agaaaccaat aatctttgtt tttctccacc ggataaatca aacggtaatt gatctttcat
     4561 tttataaagt tccatttttt ttaaaatttt ttcttgctga tctttactaa ctatttcatt
     4621 ccccagggct aactcgctcc tcactgattc tgtgaaaaat tggtaatcca tttcttgcat
     4681 tactaaaaac ggcgatgaca aatttttttt attttcgatt atctttcctt tggtagggcg
     4741 aataatcttc atgagtattc ttagtaaggt tgttttcccc acaccgtttt tccctaccaa
     4801 accaataaca tctccaactt tgacatcaaa atttatgtct tttagaatat tttcataagt
     4861 aatttcttca acttttaata catctgcact taccttttcg atatagggaa cttcaatcga
     4921 actattaaaa atgtcaaatt tccttaatgc gttattgttt tcagttttta acaactgaat
     4981 agttgtagtt tttacagttc tatttttaac aaatagaact tgatcaatta attctttcaa
     5041 atagtaaaaa cgatgttcag ctataacaat agtaaatcct ttatttttta aatcacaaag
     5101 taagtcagca agtttcttag tcgctttgaa gtccaaattt gaagacggtt catccaatat
     5161 aattaattta gagtttagcg ataaactaca tccaatagaa attttctgcc gttcaccact
     5221 tgatagcgaa aaaatgtttt tatctaatat atttgaaaga tctaataatt cagctaattc
     5281 attgattctt ttatccatat gctcttttgt aaatccaaag ttttccatcg aaaatactaa
     5341 ctcactcgtt gtatttgttg taaagaattg tcctctagga tcttgactta cgtagccaat
     5401 attttttgat atactttctt gtgttaagga ttgtagcgag ttattttcaa ataaaatttc
     5461 cccaaccaat tttccgtcat aaagtcttgg aataagtcca ttcattaact ttaatagtgt
     5521 ggatttccca ctcccagaag aaccagtaat caatgtaaga ctgccttttt ttattgaaaa
     5581 attaaggtca acaagcgctt gttccttaac atccttatat gaaaaagaaa cattgttaaa
     5641 ttcaaacaat tctttcacct caaatcaaaa tacttaagaa aaaaatagta acggaaaaca
     5701 ggatgatata gtctaataag ctcatgtttg ttttatagta agaacttctt ttcacaggag
     5761 attctatccc ctttgttatt gcattagcgg tcatttcttc agcaatcata gttgcacgta
     5821 taagaacggg aactaacaga aattccaaat attgcagagg ctgaaaaaaa gacaagcgaa
     5881 tgtttctcat ttttaaagat tctttaatca ttcttatttc agaacgataa gtcggaaaga
     5941 atctgatcac tactgtaata cttaacatta tttttttcgg taaatgtatt ttttctaaag
     6001 aggataataa ttcattgcta gtatagacac taaccaacaa ccatgagatc attacgaccg
     6061 gcaacattcg aaatacaata tataaggatg tcgcaaaaaa tatagtaaaa aactctatct
     6121 tgatagcatc aataattttc attactaaaa acagaactga ataaatcaag atgaaataaa
     6181 tacatgtctt tattttacga aacatcaata ctaaaactgt agcaataatg aaacacccca
     6241 cttgaaatat atttcccttt cctaaaaaaa taatgaccgg aaaaaaaata tttaaagtaa
     6301 tcaaggttaa cggattgatt tttgacactc tttcacctcc aaaaaataag atcgcactgt
     6361 tatcttaagc tgataaaaat gcaccgttat tcagataaca gtgcgatctt atcaaaaagc
     6421 ataactgaga acaattctca atgcaagctt tttttaatta actcagtaat tcaatccacc
     6481 caggaacaga aaattgatac aatcttctgg ttaatagcat agtcttttct tttgaatagc
     6541 tatgcttcaa tatatcatag aatgtagcgt agaatgagta aatgtagaag tggatttctt
     6601 cttcattaaa gactaaattt tgattgatca ttccgatagt ctttaactta tctagacaac
     6661 agactgtctt tttcgtcaca aatgatgtaa ttttttcaac aatatcttga tatttggttc
     6721 cagctgattg gaataaaatc aaatcgaata tacgtttatt ctcaaaaata taattaataa
     6781 atggatctaa ttcattgaag ttctctctcc aacttttctc tatatctata ttttctaagt
     6841 ttaagaggta tttggtaaac tcttcattat acatattaat aagcgcatta atatgcggct
     6901 gaataatttc ttcaaataac gcttccttag aagtaaaatg tttataaaaa gcacccgtag
     6961 ttacgcctgc ggtcaggcac agttgcctta tcttagtttg ctgaaatcct tctttttcaa
     7021 aatggcttag tccactttct attaactttt ggtgagttat aatataatct ctggtttcta
     7081 tgggtacttc ctcctaataa gatgaattaa ctttattatc ttacacagga cttgaagttt
     7141 caatcgttaa cgtttattac tgaactcatt ccttagagct aaactaatga gaaagagcag
     7201 aataactgat acaaaaagca atcctacaag aattaataga ctcatctaaa tcattcacct
     7261 tttctaaaat aatccgagaa agatttaaaa aaattatatc cagctattag gataagtcct
     7321 atcataatca ctccaatagc cattttctta tcctccctaa aaataaagtc ttttacttaa
     7381 aacctttttt gtaatcgtca ataaattttt tccctttatc agttaattga aatcctatat
     7441 ctaaatagtc ctcatatcta agtttagcca gcatctcact tacagatgct ggactaacac
     7501 ttagaacttt tgctaaatca ttatttttta atttcttttt tctttttgat aattcctgaa
     7561 ttgctagtaa ataaattagt atagtagtcg ttttaatttg taccatacct accctccaaa
     7621 ttatttcgaa aggccttcag caattttatc caggttccat ttcatcatcg catagtaact
     7681 atcaccatct tgtccttttt ctgcaattga atcagtaaaa atcgttgaat agataggaat
     7741 attggtatct tttgatactg ttttcattgg tctatcatcc acactgcttt ctacgaataa
     7801 ggagggaact tttgttgtgc gtaatttttc aactaagtgt tttatttgat ctggtgttcc
     7861 ttcttcttca gtgttgattt cccaaatgta agcagaaggc acattatacg ctttcgagaa
     7921 atatttaaag catccttcac ttgtaacaat cattttttta tcattcggaa ttgaagcaaa
     7981 tttagattta gcttctttgt ctagagaatc caatttttca atatacttat ctagattttc
     8041 tttatagaat tttttattat caggatcttt ttccgctaac tgtttttcaa tatttttagc
     8101 gtaaataata ccgttttcta aatttaacca agcatgagga tcttccttcc ctttctcact
     8161 ctgaccctct aagtaaataa catctatgcc atcacttgct gcaaaatagt ctttgttttc
     8221 ctctttgttc gcatttttaa ctaattttgt aaaccaagca tttcctccag tttccaagtt
     8281 aacaccgtta taaaaaatca aatctgcttt tgaagttttt tgaacatctt caggcaaagg
     8341 ttcatattcg tggggatctt tcccaatagg tacgatactg tgtaaatcaa ttttatcttt
     8401 tgctatattt tcggtaatat ctgctaaaat cgaattagta actactactt ttaatttttc
     8461 gttcgaagcc actttatctt tgtctttact atttattgaa catgccccga atataaaaag
     8521 agcaatcaaa atccccagac ttcccagtac tgctttccat tttctcatca taacgatctc
     8581 tccttctttc ttaaaaattt ttgttttggt gagattagaa aactaattac aaataagaat
     8641 gccgctgtta aaacaatact tgatcctgct gctatgttaa aactataacc aataaacaat
     8701 ccaagcaatg atgaacttgc tccgactgtt gcagaaataa atatcatagt tttcaagcta
     8761 tttgcatata aatatgctgt agctgctggt gtaattaaca ttgcaacaat taatatagtt
     8821 cccacacttt gcatagctgt tactgctact aaagttaata gaaccatcaa tagataatga
     8881 taaaaactga cattcatccc tattgctttt gccattaatg gatcaaaaga agtgattaat
     8941 aattctttaa aaaatagaac tactataacc aacactaaaa ccgaaacgcc aattgtaatc
     9001 cacttatcaa tatcttgtac agctaaaata tttccaaata aaatatgaaa taaatccgtt
     9061 gagctatttg caactcctat aaggataact cctagtgcta aaaaagaact gaaagtaata
     9121 ccaattgctg tatctccttt tattacacta ttgcttttta taaaagtaat aatgatagaa
     9181 gctaatagac caaatacaat cgcaccaata aaaaaattaa tacctaaaat ataagaaatt
     9241 gctactcctg gcaaaacagc atgagaaata gcatcaccca taagagacat tcctcttaaa
     9301 ataataaaac aacctactgt tccagaaaca atccctataa ctacagatgt aattagagca
     9361 ttctgtaaaa agtgaaagtc atacagacca ttaataaaat tttgaagcat attattcccc
     9421 ctctccaata aatatagtat ctccataggc tttttttaaa ttttcttcat taaatacaga
     9481 ttctacactt ccgtgagcaa ttagatttcg attcaaaaga ataatattat caaaatactt
     9541 ctttaccttg cttaaatcat gatgcacaat tagcactgtt tttccctgat gtttcaattt
     9601 ttttaacgta tccataatta ttcgttcact aactaagtct atccctacaa atggttcatc
     9661 taaaaatatg aaatccgcat tttgagcaag acatcttgct aaaagtacac gttgaaattg
     9721 tcctccagat aattctccta tttgatgatt agaatatttt cccatatcga ccttttccaa
     9781 agcctttgaa actctttgcc attctgcatt tttaattctt tgaaaaactt tcattccggc
     9841 atatgttccc atagatacac attcttttac ttttattgga aaagtaaaat caatatcagc
     9901 tttttgctct acatatgcaa tcttcaccag ttttttattg atatcttcat tattaatgga
     9961 gacctctccc tgatgaggga taatatttaa cattgcttta agcaatgtgg attttccagc
    10021 accatttgga ccaataatac ctgttaaagc accttcttgt atttttaaag aaatctcgtt
    10081 caatgctaaa aaatcgttat aacaaacatt aagctttttc acttctaaca taactttctc
    10141 cttctaaatg ataatgatta tcacgatcat atggtataca aaaattatat tttagtcaac
    10201 cctaaatttt atagtttaga tttttttctg ttgctgaaga ttagaatgac aaaactattt
    10261 gaaactacgc gtatagtaag tagctttcaa aatatataaa aaaggagcgt tctcaaatga
    10321 gaacatgctc ctaaaactac tttattggcg tatggaaaaa ttatacttga agtggatagc
    10381 gggttgaata agttgataag catcaaaaca tttataaaaa ttattcaaga ctatgtctgt
    10441 tccatataca aatatgaaaa tcagtaaact ccatttaact tttttcgtag tgtgtctttt
    10501 tatacatgtt tatcgttatt aatagtgaat aggaaaaaaa ctatttttga tcattgacaa
    10561 ttaaatacaa tttttgaata acgatccctg ctattcgagc tagaaactca agtacgccat
    10621 gacctgatta gcgacaaata cttagagatt tctttttgtt ctgggaaaac aaaatgccat
    10681 gacaatagca gcaaataatg atacttccgt aaggctcgcc gtaaactggg gagaggtgaa
    10741 attcctatgt gcttacccaa agcaattcaa aaatttcata actactattt taggagatga
    10801 ttttatagaa tccagagtct acctgaataa aaaacaaacc tgtgcatttc taggaatcga
    10861 taataatact ctagataagt ggattcgtat atataatttc ccagtaataa gagttggtgg
    10921 tgtagttcgc tttgacgtag aagatattaa agactttatg aatacattta aaataacttg
    10981 aaattttctt ctcgccgcat atactaaaat tagtgaaata tactgcctta ctttttttaa
    11041 ggagtgagga ttttgcctac aaagctttca aatgggaaat ataaaactaa tctgagatat
    11101 ccaaaaaggt ttaaagaaat aacaggaatt gcatcagaaa agtatcaaaa aacatttcca
    11161 aataggcaat tggcgattaa agccgaaaat gatatgaaga agaaaataga aaaagttttg
    11221 agagaggaga atgcaaactc attagaatta aaaggaaaaa taacgtttaa aaaattttac
    11281 gaatctaaat ggcttcctag atatgaatta ggtcaaacaa ttagatcaaa caggcctcct
    11341 agtgatataa ccattagtaa tacaaaagat atatttcggt tgcatatttt acctatgttt
    11401 ggtgaatacg caatgaatta tctaaatatc aatactgaga taatatcaga tgaattaacc
    11461 aaaaaatcga aagaatatgc aaatataaaa atcataaaag gatatgttcg ttccatgttc
    11521 gatattgcag aaatactaaa ttatatcgaa tttaatcgaa ctactaaaat tattcaaagt
    11581 atcactgcac ctaaaaaaaa tgctttagaa gaaaaaagaa tacaagaagg taagcaagca
    11641 ctatcatcaa aagaacttac taattggata gaagcagtaa atgatgattt taataatcat
    11701 ttacttactt ttcatgatta cacacttttt atgattaccc tgtatcttgg tgatagaaaa
    11761 agtgagactt acgctttgca atggaaatat attgattttg aaaaacaaac ggtccgtctt
    11821 aaacatactt tagataaata tcaaagaaaa aagtttacaa aaggtcgaaa agataccgtc
    11881 attcaagtac cagaagttgt aatgacattg ctaagtgaat ggaaatcggt acaagctgat
    11941 caattgttga aattaaaaat taagcaaacg ttagatcagt atctctttac ttatactaaa
    12001 ccatctggag aagtaaattg tcctgttcat gccgattacc taaactatcg aatcaattca
    12061 attaaaagaa ggcatcctga cttagttcat ttatctccac acaaactccg tcatacttat
    12121 gcaactatag ctagacaagg tggagcagat atgaatcaaa tttcaaatgc tttaacacac
    12181 tcagatattt ctacaacaaa gatatatgtg aacacaccag atatagttga taaggctgtt
    12241 tttgaagctt ttcagagagg attgaataag tgtgattgat atgtgttctt aaatgtgttc
    12301 ttaaaaacaa aagaagcact ttccaaataa ttgaaaagtg ccagaaacgt tgatatataa
    12361 aggatattaa cgttttgaga attgtgaagc tttacgtgct ttcttaagac ctggtttttt
    12421 acgttcgacc atacgtgcgt cacgagtaag taagccagca cgtttaagag ctaaacggaa
    12481 gtcaggatca acttctagca atgcacgagc gataccatga cggatagctc ctgattgtcc
    12541 agcgtatcct ccgccgttta cgttaacaaa tacgtcatat gcgccttttg tttcagtaac
    12601 agcgaaaggt tgattgatta cttcacgcaa gtcagcatgt ggaatgtatt cttcaacatc
    12661 ttttttattt acagtaattt tgccagttcc tggtactaga cgtacgcgag caactgcgtt
    12721 tttacgacgg cctgtgccga tatattgaac ttgtgccaat gaaattccct cctattagat
    12781 taagtttgtg atgtctaaaa cttctggttg ttgtgctgca tgtggatgtt ctgctccacc
    12841 gtatacgttc aattttgtaa attgtttgcg gcctaaagtg tttttaggaa gcataccttt
    12901 tacagaagtt tcgatcaaac gacgagaatt tttagcacgc aattcgccag cagtgattga
    12961 tttcaatcct cctgggtagt tgctgtgacg gtaataaatt ttgtcagtcg cttttttacc
    13021 tgttaatttc actttgtctg cattgatgac gatgacgaaa tcgccagtat ccaaatgagg
    13081 tgtgaaagtt ggtttatttt taccacgtag tacagatgca acaactgttg aaagacgtcc
    13141 caatggaaca tcagttgcgt ctactacata ccatttacgt tctacttcgc ctggtttggc
    13201 catatatgtt gtacgcacgt tattttcctc caatttaagt tctgatgttt gacatacact
    13261 tcgagtttcc ggggctcttt gtggggcaaa caataccatt taacatatta cccgctaatc
    13321 tagtcaatgt caacgttttt ctgcttgatt gaaaaattta tatgaaactt tacttctttt
    13381 ctttctttta tataaaaggg tcgtttcctg tcaaaatgaa aaacaaaaag aggctgtgac
    13441 aaaagtcttg tcacaacccc ttattttgaa taaacggtgt tctcctgcca actcctcggt
    13501 cacaagtcac aatatccata aaacataaga agctgttttt taaaattgtt tcttgtgact
    13561 cggagctgaa cggcaggctt acaacctctt ggtaaacggt gttcaatcag ctgaccctcg
    13621 gtattagctc aaaaaacggc aaaatatgag gaactatttt gacgtttgtt tctctaatac
    13681 tcaggtcaaa acgctgattt cacaacctct ttttcggtca agttcaacaa cagtttttac
    13741 gcgtcgatct ctggcgcttt gcccaaatga gcttggatca tccagatacg tttttctgtg
    13801 gctgttttga attcgataaa gatatcttgt gttggatcgt cgccttcttc tccagaaact
    13861 tcgattcctt tttggtaaag atctgctaga taacgatagc ctgcaacaag tgtttccaaa
    13921 cgttctggaa tagtacgatc ccagcggcca atttcatcag ggatttttgt gttatctgca
    13981 aattcacgca atgtagaata aggtgaaccg tctaatgtga tcaagcgttc ggatacttca
    14041 tctaactgag cgttcaaatc atccatgaac tcatccatca ttgggtgcaa tgtaaggaaa
    14101 cctggtcctc tcatgtacca atgtgtttgg tgaacaacaa cagacatttg gcttagatct
    14161 gcaactaatt gatttaatac ttctttggtt tgttggaatt tcatctttct tgcctcctaa
    14221 cattttctta cacttaaaat aatagtagac tttcaatgaa aagtacaaca aaatcattta
    14281 cctgagaacg attttcagtt cgctttttta tccgtaaagc caatcatttt tcagaatcag
    14341 ctaattcttc tcgcagttga gcaaattgct gataagtctg tgctaaatgc ttttgtgtcg
    14401 aaacaaataa tggatagatt ttctgatatt ttttatgatc agtttcttgt actccaactt
    14461 gctctttcaa ttctatcaat tcctgtgctt cactgatact ttggatcttt ccgatgcttc
    14521 tccagcctaa aatcacagct cctaatgcgg atgcttctgt gacagtaggg aaaacaagcg
    14581 ggtaacccag tacatcagcc atcatctgac gaaaaatcgg agattgagca aacccgcctg
    14641 tagcacggat ctctgtgagc tgattttcct ggcaaactcc ttttaaaatc cgataaaggt
    14701 tgagacagat cccttctaga atcgccttca tcatttcttt ttctgtatgt tgccgctgta
    14761 aaccgataaa actgccaaac gcatctgcgt cccaaagcgg tgctcgctca ccaagtaaat
    14821 agggatgaaa aatcagacca ttggcacccg ctggcacaaa agcgatagcc gaaagcagtg
    14881 catcatatgg attttctcct ttttctttag cagattgaac gacttcctgt aaaaatgtat
    14941 gccctgccca atcaaagatg ccagcaccat tgctagtcgc tcctcccact acccaatgtt
    15001 tctgatccag aacataacaa aacgtctcca tttctggatg gaactgaggc tgagtagtaa
    15061 taaaacgaag tgctccgctc gttcctacgg tcaaagcggc gatcgtatca ccaaaagcac
    15121 ctaaccctag attagaaagc ggcccgtctg cccctcccaa aacaaatgat atatcatttg
    15181 gcaaacctag ttctgctgct gtttttgatg gtagacctgt tacttgatag gtggaaggaa
    15241 agacttgcgg caattgctct attcgaatgt tcaagtagtc aagcacttct tctgcccatt
    15301 ggaattcatg gatatcaaaa tatccagttc ctgaagctgt tccgtagtcg ctgatcagtt
    15361 cgccaaacaa tctataaaag atatagtctt tgataccgac aaaataagca gtttttttga
    15421 ataattcagg ttggctgttt tgcagccact tgattttgtt caatggtgtc attggatgaa
    15481 tcggcgtccc agtctgccga taaaattctt tgccaagcac ggtatatttc agtttttctg
    15541 cttcgtccgc tgctcggtta tctgcccaag tgatcattcg agataacggt gttttctctt
    15601 catccaacaa tatcaggcca tgcatcgcag aggagaagga gacaaattga atgattgtgt
    15661 ctgaaggcaa ctgtttaatt acttctttta taacggtgca gacaccttga aagatcaatt
    15721 cctgatcttg ttctgccatc ccttgtgttt cttggatcaa agggtagcga aaataagcgg
    15781 atgctgcttc ttgggcttca tctgtgaaag caactgcttt ggtttgtgtg gttccgatat
    15841 ctaatccgat tgaaacgttc attttttcac ctcaaatttt atgtatgtac aaaaatttag
    15901 tttaagcttg cgtatcttgg ttttgaaaag ggggagatgg cgtgttactt tattttatca
    15961 ttggttgctg tatcggatca tttttatgtt taaccgccca gcggattccg ttaggtcact
    16021 ctattattta tcctagatct cattgtgtca aatgctgtcg ttcgctttct tggtatgaat
    16081 tgattccgat tctctcgatc atcgtccaac gatttcgctg ccgctattgc aggtgtcggt
    16141 tgcccttcta ctatttgttg gcagaagcct tatgtggtgg tctttttgcc tggttcttta
    16201 ctttttcttc cgcaagaaat ttttcgacgt tcatatggat gctttcggct ttgctatttt
    16261 cgttgatgga tttattttac ttcatggtcc attctcacac gctattctgt tcttgggcaa
    16321 ttttatggat tttttggttg caggccggcg tcttccaatg gcaaagtgtg ctgttaacgt
    16381 ttatcgtagg cggtgtgtgc cttcgctatg gtcaatccta tctcggagct ggcgatactc
    16441 tgttgatgct ttcttggagt ggtggcttgt ctctagaaga gttgcttcag atcttgtttc
    16501 ttgctagtct gtttggaata tccgtttttt ctgtttactt tatctgtcat aaaaaaaagt
    16561 tggaaaagct gcctttcatt ccttttttga gcagtgcgtt gctgattgtc cttcatctta
    16621 ggtattgaaa aaaggttgtg acaaacgact tgtcacaacc tctgatatat agtgttctct
    16681 taccaaatcc ttgtgacttg gagctaggcg acaggttcac aacctctcca ctaaatggtg
    16741 ttcaatcagc tgaccctcag tattagctca aaatgctggt tttacaacct ctgatatacg
    16801 gtgttccata agtagctttt tcggcttctt atttcttgac gccccaatgt cgacgaagga
    16861 aacctgcacg tccagaacct tctcgataaa gattgtaatg gagcttgttt ttcttgtaat
    16921 aatcctgatg gtaatcttct gcaggataaa attcgcttct tggttcgatt tttgtcacga
    16981 ttggcttatc gaatcgcccg ctgtcttgca atgcttgttt tgaacgctct gcaatatttc
    17041 gttcttcttc atcattatag tagatcactg gacgataaga atcaccgcga tcagcgaact
    17101 gtcctaatgc gtcagtcgga tcggtttgct gccaatagat ttccaccaac tgatcataag
    17161 aaataacttc tggatcatat tcgatctcta ctgcttctgt atgccctgtt gtgcctgttg
    17221 ttacttgttc ataagttgga tttggtacat gcccgccagt ataaccggat gtcacagaaa
    17281 tgatccctgg ctgctcttca aatggtttga ccatacacca aaagcatccg cctgcgaaaa
    17341 ctgctttttt tgtcatataa aatccccctt ttttctcatc ataccttgat ttccaaaagg
    17401 aggataacgt tttgcttgtg acagaaaaaa cggaaactat tatatacgtg atacggcttc
    17461 tgataaatgc caaaacgaac gtacttgatc aaaaacggtt aaatcaggaa tcacatattc
    17521 agcagaagga agatttatgt tttcacctat tcccaataca tgcatgttac cagccaaagc
    17581 tgcttgacaa ccagcttctg aatcttcaat gaccaagcat gcatttggcg gtacattcaa
    17641 ttgctgtgct cctttgagaa aaacttctgg atcaggtttt gcttttgata cttgcgtgcc
    17701 gtcaataagg acatcaaaat aacttgtcaa attcgttttt tctaaaatga ggcgtgcatt
    17761 tttgcttgct gatcccaatc cgatcttgat gtttgttttt ttcaagtact caagaacagc
    17821 tattgcacca ggcaaaacgg atgtttcatc cattttttga atcgcttgaa gatatagatt
    17881 gtttttttgc tcagcaaagg ctgatttctc tttttctgag aatttctgtt ccatttttcc
    17941 aaatgacagc aatcgctcta gtgactcggt tctggatatt cctttcagtt gctcgttttc
    18001 tttctcagtg aatggaatgg acagctggtt agctaaaacc agccacgctt cgtaatgata
    18061 acgagctgtg tctacaagaa cgccatctaa atcgaatagt gctgctttga ttttcatcga
    18121 ttacctcctt gaaaatgaac gacttgaagt aaagatgcga tgtttttagc tgtgcgatga
    18181 agattatcag tggcttgctg taaagcttct tgtaaaggca cagctgcagg aaggatcgga
    18241 aaaacggccg aaatgccaac atctgctgct ttttcgcttc ctttaccaac acttccgcaa
    18301 atagctatca caggaacgcc ctctggtact cttctagcaa tcccaactgg cgcttttcct
    18361 tttagacttt gtccatctag acgtccttcg cctacgatta cgaggtctgc ttgctgtacc
    18421 cgtttatcga actccagtaa atccaatacg tagtcgatgc cagagactat tttccctgaa
    18481 gcaaatgcta acagtcctgc acccattcca ccaccagcac tactgcccga tgcctcgatt
    18541 acttgagggg caaatctcat ataaaaatca tgcatggctt ggtcagccac tgctaattca
    18601 ttttctttca agcctttttg tggtccgaaa acaaaagaag cgccttcttt tccgcataaa
    18661 agattagtaa catcagtaat gacagtgatt ttggctttaa tcagctcaat aggcacgttc
    18721 gttttatcaa ctgtagcaat tttacctaga ttttctccca gtgcttctac tttttgatta
    18781 tcttgatcgt aaaaagcata acctagacct tctgccatgc cgatgccgcc atcgttggta
    18841 ctacttccgc caacgccgat gatgatgtgt cgaacacctt gttgcaacaa ttgtcgtatc
    18901 agttctccta ctcctcgcgt ggataaccta agtggctgtt gttggtttag tgggacatct
    18961 ttcaatccga cgatttctgc catttcaaaa atcgcagttt gaccgtctga agcgtactgg
    19021 atttctttgt ttttgccaaa tgcattagta acgactgctg tttgccgctt caaccttaaa
    19081 ctgtctgcta atgatgaaac cgttccttct cctccatcgc ctataggtag cagatcatat
    19141 tcagcctgaa caaaaacttc ttgaaaacct gattgaatgg cttgtgctgc ctctaaggct
    19201 gtcagacttt ctttgaaaga atctggcgca atcagaatac gcattatttt cccctccgtt
    19261 ccaagatcag ttctttttcc agctggtatt ggtctccatt gacaaaaata ggtaatgttt
    19321 ctttcgtctc tttttttatt actatttgtt tttttgtcaa aacaaatagc aacctgtgat
    19381 ttttccaaat aaaaggaaaa gaaatttttt cccatgctga aggaagtctt ggagaaatag
    19441 aaagagttcc attcgttact gtaatacctg aaaaaccaaa tataactgct agccatagtg
    19501 ctcctaatga agcagcatga attccgtcat ctgatgaatg cggatctggt cctaaatcaa
    19561 tcaggcacgc ttcttggaat aaatgatacg cccattccat ttcttggata cggcaagcct
    19621 ctatggcgtg aatcgcttta ctcaacgatg agtcgtggat tgttttgttt tcatagtaat
    19681 gcaaattctt agaaacgatt ggttgagaaa aatactcagg taataagtat aacagcatga
    19741 caagatctgc ttgcttgctg acttggagtt catttacttc gctacgggca taatcaagta
    19801 aaatagactg gctgccagga cgttctttgt aagtggtcaa atcaataact ggcttactga
    19861 gatacgtgtc atcttgcgga atcagctgtt ctttgttagg cactggcaaa tataacttat
    19921 ctaaaaaatt ttgacattct ttcaaaaaaa gcggatcgcc agcttgatat ttaacagcta
    19981 aggccacatt ataatgcgcc agatagttcg tatatgcatt attgtcgata tattctgtgt
    20041 attcatctgg accaatgact ctgtgtatct ctaaatgatc atcgactttt acagctctgc
    20101 tcagccaaaa agctgctgtt tcatgaatga gcgtttttcc ctttgtttct aaaaacgatt
    20161 gatcctctgt tgcttggaca tactcttgaa cggcataagc aaggtctgca ctgatatgat
    20221 gttctgcggc agctgaagca acttcttgtc ttgttccggt tttgatattc aatgcagcaa
    20281 atttgggtgt ctcttcctga ccagtgtgtg cgctttccca tgggaaaagt gcaccttgat
    20341 agtgataatc tcttgctttt tgccgtgcgc cttctaatcg atcataacgg tacagcagca
    20401 aattctttgc tacttcagga aactgatata aaaagaaagg caggataaaa atttccgtat
    20461 cccagaaaac atgcccttta taaccttcgc cagttagtcc cttcgcccct acactaacac
    20521 ttggatcatt gcggggagtc ataatctgta aatggtaagc agcgaagtcc atcgctaact
    20581 gatcgaaaga agaatcgcct tggatcgtta tccgatgctg atgccaaaaa tctgtccaat
    20641 aagacatcga ctgctctaaa atttctgtat aactaacgct tttctgggct tgggcaatac
    20701 ttttcttaaa cgggtctgtt tgctgaagag aggtagagag cacgatgatc ttctccagcg
    20761 taaaggacgt attttttctg atcttttctg aaaaggaagc ggtgatccgg cgattttttg
    20821 cttggaatac acctgcttta ttgaatttag ctgcaatccc aatatcaatt ttactttctg
    20881 ttgtctggta ggatgcactg atcaaatcgt caccaaatac tcgcagttct ttttcaacca
    20941 actgctgtgt accaaaattg gtttgctgtc catcgatacc cgttttcaca tctacttggc
    21001 agtcttcggt caacggcttg atgaccactt tcagggctgc ctgatggaga ttattttgat
    21061 cagcaacacg acaagtaatg acttccactt tttttccttg agcagtttca atgagcgcat
    21121 agcgtttaag ctctcccatt tctaaatcta gtgcccgctc gtatgcaaga actttcatcg
    21181 tctcaagaga aaaatgttca ccgtcgattt ttatttccca tctgatgaca tctgggagat
    21241 tcactaactc ggctgattct gctcctaaag gtcggttata tacaccagct gcaaacattc
    21301 cccttacttg attccgatag tcttcctcct ggcttcctct gataccaaaa aatccatttc
    21361 caatggtcat ctgactcgca tacttattga tatactctaa atcaaactgt ttttcttcta
    21421 ctttcataag gtaataactt ctcctgtttc tgccgaacga tataaatttt ccaccagatt
    21481 ctggatgaca tatccttgcc agccatctgc aatcatctga gtctggtcac cgagcgcccg
    21541 attcacaaaa tgagccatac ttagttcatg tgggtcatta actggcgtgg tcttttgtaa
    21601 ggaaaccagc tttccatcta catcttcgaa tagttcgcct ggatacagat tagcacctgc
    21661 ctgatcgcca caaaaagcca cattcagttt gtcttctctg atattcaaag caaacgaagt
    21721 attcagttgg atgatcttgc cgttttctaa ttccagtgtg ccaaaaattg catcttctac
    21781 tgtgaatttg cttggatccc atttcccgaa tttccctgaa ttttttttcg taccaatttt
    21841 ttggaagctg tacgcccgaa cgctttttac tttaggaaat ccagtaatat acatcgctgt
    21901 atcaagcatg tgaatgccat aatcgatcaa aggcccgcct ccttgaagtt ctttgtcgat
    21961 aaacgttccc cagcctggaa caccagaccg acgaagcgca ttcgcttcta cgtaataaat
    22021 atccccgaga acatcaagat gattcttgat cattgttgct tcgtcagaaa aacgatgctg
    22081 gaatccataa gccaaaatct gcccgttttt atcggctgtc tctgccatct ttcttgcttg
    22141 atcagctgtc atagctggtg gtttctcgca catcactgaa gcattagctt cgagggcagc
    22201 caatacatgt tcgaagtgaa agcgattggg tgtacagaca ctcacgatat cgggagaaat
    22261 ctcattcagc atttgtgtaa ctgatgtata cgcatgagaa aatcctgtac gtcggcaaaa
    22321 gtcttctgct cgctggctga ctggatcaac cacagcggcc aattctaaga catctgatct
    22381 tttttgataa taagcagcat gtactttttc tgctacttgt ccagcgccta tgattgctac
    22441 tttcttcatt tatttctccc tcctttgctt atttacttat tgttattctt tctctattct
    22501 ttctctaaat catttaaatg acgcgctgtt tccagacagt ttttcaagta ttgcaatgat
    22561 tcctgataaa ttttcacttc attttctcct cgcaaacgac attcatatac catataacct
    22621 tcatactcat tttgaagtag cgtttgaaaa tggcgagtaa aatcaattgc gccagatcct
    22681 ggttggtagc gatgattatc tgctaaatgg atatgaccca catactcttt ttgcgtagtc
    22741 aatgcttttg aaatatcatc ttcttcaata ttcatatgat aaaaatcagc gatgatctgc
    22801 acatgtttca attgattaga gtcaatgtaa tgacgcgcat ccgctaacaa attgatcata
    22861 tggtcttggt atcggttcaa aggctccaaa taaataaccg tacctgtttc ttcggctact
    22921 ttatctaaat aacgaagtga ttcgctgact gctttatagt ctccttcttt gcttcggggt
    22981 gaaaccatcg gcggtaatcg ataagtgaac attccccaag ctgctgggat aacgattcct
    23041 ttgcctccaa cctcgcttag tgcacgcaaa atctcagcaa tttgacgtac accattaaga
    23101 cgccgttctt cgataaagtc accgatccac ccatcgtagc cgttacaagc tgtcactact
    23161 gggatccctg tttctttgat tgcctgtttg actgtgtcga tattttctat taacaactta
    23221 ccgtcgattt cgtattcttc aaatccacag tctttgataa atctaaattt ttctaaaatg
    23281 tctcttggga aaaatgcttg atcttgcgta ccgaatttca ttgttctcct cctcggactt
    23341 gaacgcccat tttgatactt tcttctgggt gctggtctac ataacgcatg taagcttctg
    23401 cgctttctgc aaatggcaca actggatcaa tgatttcttc acaatccaag taaccgttca
    23461 tcaataattc ccaacatgtg tcctcgattc gtttacggtt ccatcttggg taatctgggt
    23521 ttggttcact agatgctcga gcaaagacga tttttgcatt attgaaatgt gcttcgcgac
    23581 caagattaaa cccagctgga aaaggtttag caaaggcaac ataagaaatg atcccgccat
    23641 agccgatccc tcttaaagaa gcttgtaatg cggcagcact gccactcgtt tcaataactg
    23701 aatctactcc acgtttatct gtcaattcct ttagacgaaa accgacatct gctttcatag
    23761 gatctagcgt gtaatcggca ccgtattttt ctgctaattc tctacgctgc gctatgggat
    23821 cgatcccaac gatggtagta gcacctgctt ttttagctaa ttgaatcgca atttgcccga
    23881 tagcgcctaa accgaagatt gctacagcat cacctgctcg aacatgtgct tcccggatac
    23941 cacctaaagc gaattgagca ggatcgtaac atactgcatt tttccattga tccaaccgat
    24001 caagtttacg tagtttataa ttatcaacac cgtttgtaat cagcgtttca cgaatgggtc
    24061 cataagtgca gactaactct cctttttgat actctgtcac caaactgccc gtttcttcga
    24121 tcgtaccgac aatcatattt cctaattgaa attcgccaaa taccacaccg cgttggcttc
    24181 cttcttctct tggcaagaat aggcgccatt cttctgaaaa ttcttcatcg ataaatggac
    24241 ttaatcctct aaagtcgaca atttctgttc ctggttttgg agtagcgaaa attactcgga
    24301 tacgtacttc gttcttttcg atctcacggt cttcgtaatc tttgaatccg gctattcttg
    24361 gttctaatgc aactaatttt ttcatctttt ctttcctctt tcttttcttt ttatcctttt
    24421 acgccgcctt cgactaaatc acctttaata aatcgttcag ataacgcata cataatgatg
    24481 actggtaaag cggtcaccaa tgaagcggcc atcattcgtc cccaaatgta gtctggtgta
    24541 ctgaataatg tgttcaaacc gattggtaaa gtaaattttt cggaggtcga caggaaaatc
    24601 gaagcaaata aataatcatt ccacgaaacc ataaagcaat aaacgaagac agaaatgatt
    24661 ccagaaatag ccaatggtat aatgatccga ataatgatct ggatccggct aagaccatcg
    24721 atcattgctg cttcttcaat atcagtcgga atcgtgtcaa aataactttt tagcatgaat
    24781 atcgcagtag gaagtgtttg aacgaccatc gtgataatca gtgctgtatt ggtatcatac
    24841 aaacctagac cggaaatgat tttaaataat ggaacaacga tcaaaatacc ggaaaacata
    24901 taaacggtgt aaaagctagc attgattttc gagcgagctt tgaagcgtag ttttgacaaa
    24961 gcgtatgccc cgctgatccc aatcactact gcagttacgg aggctatcaa ggagatcact
    25021 aaactatttt tgaaatacat gatgaatgga aaaatctcag cattgaaaat atcctgataa
    25081 tgttcaaaag tccattcttt cggaaaaata gtttgtgtcg ttgcaattgc ttcttgtgaa
    25141 tccttgaatg atgtcatcag catgatcaaa aatggaaaca aattgacagc taatagcaac
    25201 aaaatcgtcg cgtaaaataa gattttcctg ctctttttat ttttcatttc aagtccacct
    25261 tctttcgtga gagcaagacg accgctgcaa taatgatgaa caatagaatc gatatagctg
    25321 ctgcttttcc aagatcattg aacgcaaagg ctgtctgata caaataaacg cctaagatat
    25381 tgactttatt cgtcagtaga tagacatctg taaacatata gaacatccag atagatcgta
    25441 aaacaatcac agttgctagt actggtttga ttgccggcag tgtcacgatc ttgaacttct
    25501 gccaagcatt tgcaccgtcc atttcagctg cttcataaag cgttttgtcg actgtttgca
    25561 aaatagccaa aaatgaaata aacgcatacg gatagtatct ccagatagca aacaaaacga
    25621 ccataaagaa gctagcaact ggctggtcga accacagcgg tgcttcgttg aaaagctgca
    25681 acctatctac taacagatca ttgacgattc cataactatt attgaacata tatttccaag
    25741 caaaaatcaa agaaatagat ggcgtaacat aacttaataa aatcagtgaa cgcaccgttt
    25801 tcctaaatct gaacttgcgg ttaagcaaaa tagctacggc taatcctaaa gctgtactgc
    25861 ctgctacaac taagaatgtg taagcaactg ttaaacctaa agaacgataa aattcactat
    25921 cacttaaaat ttcaagataa ttgcttaatc cgataaaaac ggcgtctgag ttaggattca
    25981 gcggcatatc gaaaaaactg atctgaatat tggaaagcat tggaatcagc actaaaacag
    26041 ctaataaaag catgcttggt gctaaaagtc ccatggctaa ttttaagtca gaatgtttcc
    26101 ttggacgatt cattgaaacc tccccctgat aatctgccaa tagctatcta cttttttcga
    26161 acaccattgg cagaacttct tgttatttcg tatttttttg tgcgtcagct aaagcttgat
    26221 cgacatcttc gtttccgaca gttacttggt tcactgcttt tccgatcaca cctgagcttg
    26281 tgatatctcc cattttcgtg aaattctttt ccccgactag tccaaagact tgtacttcat
    26341 caaaagcact agcaatctca gatggcagct cgccaaaaga cttgatgact tcattttctt
    26401 tgtaaccatc cagttcgact acttgtttat tcactggttg agcaccacca ggagacatca
    26461 agacccactt ttccatgttt ttcggttgag ataagaaagc tgtgaatgct tcagcagcct
    26521 ctttttgttt ctcgtctaga ccagaagaaa tcgtcaatcc agatactgtg ccatagacag
    26581 cttgatcttt ttgtgttggg atagcaaaac cgatattttc tgagttcccc gcttcataaa
    26641 cagaaggcaa aatgtaagtt gaatagattg ccattggtgc tgttccattc atgaaagcat
    26701 ctttgatttc tgtcacatca ttagagcctg gcatcgtgta ttgggataag ttttgataaa
    26761 acgacaatgc ttctctcatc ttttccgtat cgatcgttac ctcgccttca tcatccaaaa
    26821 catttgctcc atttgacaaa gcaaattgag agaacgcttg ctcactcatt gttccttcag
    26881 cagtaggcat agcaattcca tattgtttat tctctttatc tgtgaaatgt tgagcaattt
    26941 ttaaaatatc atcccaattt tctggttctg aaaaaccagc ttctgataat ttttctttgt
    27001 cataccaaat accttgaacc cagccgctaa ttggtgcagc aatatagctt tttccatctt
    27061 ctgaacgaac aaggtttttg gctccgtcat aataattatc ttctccagct tcctcgataa
    27121 cgttttggac tgccgtctga tcaatcagtt catctttatc catcacttta gcaaaatcct
    27181 gactgacttc cattacttct ggtaatttac cggaacgagc taacgtaacg atttttgtat
    27241 tgaacgctga ttcttctacc ggcacttgct tgattttgat gtctggattt tctttttcaa
    27301 aatcagcaac cagttcattg atcacactta atcgttcctg ttcgacagaa gagtgcatga
    27361 attgaatcgt tgtctgttgt gattcgttag agctttgact ttcaccacaa gcagtaagaa
    27421 gaaatccagt agctagcaga caggtagtaa caataatttt tttcattttt ttccacctct
    27481 tattttatta ttttcagcca catatattca tacggctgga atatcaactt tcgttcttga
    27541 ctgcaatgga taatttgtcc agaaaacagg tctaggtaat ctccttttgg taaaagataa
    27601 cctgctgatt gattggaaaa attatggata actaagactt tctcttcctt cgcttcgcga
    27661 ataaaactga taaaagaatc tgctgagtcc agtaagtaaa tcaaactatt cggatggaag
    27721 gctttttctt tttttcgaat agatagcatc tgggtcaatt caccatatac tgctgttctt
    27781 aatggatctg tctgcaactc tttctcaata tctgctaaca aaaatttttg ccgattgatt
    27841 tttcgattct ctcctgtttt ttctacgcct ttgtaatcat ttcgacttcc cagcacactt
    27901 tgaatataaa ttgccggcac gcctggtaac gttaccaaaa atgaatgagc agctaaaaag
    27961 cgtttgcttc tccattccgt cgtatctgtt ggagaactta acgcatctag atacgtagcg
    28021 tttatctcat atggactggt cgttccatct gggtttttct tgtagtttac tcgggtaccc
    28081 tctttcttca gctgttccac taattcaagg atcgtttttt ccggcagaat acctcgtacc
    28141 ggattcaatc cgataccgtc atgggaggct aaaaaattaa aaaaagttgt ttttttcgat
    28201 gcgaaatcga tggtttttaa ccattctttc aacttacttg tattacccga acgaatcgct
    28261 tgaagggtca aaggtggtag cgaaaactga tagaccatct gtgcttcatt ctcgccattc
    28321 ccaaaataac tgatattatc tttgtgtggc acatttgtct cagtgatcaa taccgttcct
    28381 ggagctgctt catcagcgat tgcacggaat aattggatga ttttatgcgt ttctggcagg
    28441 tgtatacaag aagttcctag ttctttccat aaaaatccca cagcgtccaa gcgaataaat
    28501 gaagcgcctt tttctaagta aaacaaaaga atatcgatca ttttcaataa aacttctgga
    28561 tttttaaaat tcaagtctac ttgatcttcg ctaaaagtcg tccatacgtg tttttctttc
    28621 ccgtttttgt caatgaacgt tgtaagtaac gggctggtcc ttggtctagt cacgctgctt
    28681 gtatctgtat ccagtggcaa atcaataaaa aaatctttgt acgtatcatt caagtttaaa
    28741 tattcctgaa accaagtact ttcggcagac atatgattgc agacaaagtc gaacattaac
    28801 cgtgtttcgc ttctcatttc ttctatatct tcccatgtgc ccaaacggtc gttgattgcc
    28861 tgataattta caacggagaa accatcatca gatgtatatg ggaaaaatgg caagaaatgt
    28921 acaactctaa atgtatctgc caaatacttt tgataaaatt tcttaaatgt acgcaaagtt
    28981 ggttgctgct cttcaaaaaa ttgatcccca taagtaatca agaaaatatc ttcttcgtcc
    29041 caatgcagtt tgcctgcctg gcctttcttc tgctgataaa cactgacttt ttgttcaatc
    29101 atagaggcgg ttttctcaga caaatctgag tcgcgataaa tcgcttgtaa aagttttgta
    29161 tacatcggat tcccctttct tttttgggag cgttcccaac gatttgatca tattatggaa
    29221 aacgcttact gtcaatagca tttttttaaa taattttgat atacacaaaa atacaaataa
    29281 gaaagcttat gtatcaataa ttaatagctt cttaaaaaaa tatggtctct catgatcatg
    29341 caagctattc ctaaattttc aaaatcatag acaaaaaaga atgtctattg tactatggaa
    29401 ttattctaaa acgctcccaa aaaggaggtg catataaaat tgaaacctac tatttatgac
    29461 attgcgaaaa aagctaatgt ctcaaaatca actgtctcca gagtattaaa taaccagcca
    29521 aatatctctg ataaagcaaa agcaagtgta cttcaagcaa tcaaagaatt gaattatgaa
    29581 ccgagcaaaa ttgcaagagg actgtcagga aatggttttg acgcgattct agcattatct
    29641 catcgtactt ctcatacgac ctatggcaat cctttttttt cagatgttat tcaatccatt
    29701 tctgccgttt ctgaacaaaa taacttcgac ttgattctac aaacggcaaa aacaccagaa
    29761 gaagagctag aaaaatgcgc gaaaaagatc caagaaaaaa tgatccgagg aattattatt
    29821 ttaagctcta cgatggatga aacacttctt tttgaattgg atcaatacaa tattcctatt
    29881 gtggtaatcg gaaaagtcgg aaacgactac acgaacatct actcagtgga cacggataat
    29941 ttccaggata gttacgattt ggtggaaaca ctgatcaaga atggacaccg tcatatcggc
    30001 tgtctccatg ctccgacaga agtttatgta tccaatgatc gtctcaatgg ttaccgtcaa
    30061 tgcttatttg atcaccacct agatatccgt aatgaatgga ttatagacgg cggctttcgc
    30121 ttagaagata gcatggcagc aatgaatgat ttaatgaagc tacgagaaag accaactgcg
    30181 attttttcta ccgatgcatt aaaatcattg agtatttaca aagtggcaga acaatctgga
    30241 aagcagatac ctgatgatct ttcgatcgca ggattcagtg atcccttatt gtcctcccta
    30301 ttttcgccac agttgactcg gatagacatt ccgacaaatc agttaggaat caaagccaca
    30361 gaacttttat tccaactaat ccaccaccaa taccctgaag aaaaaagaat cattctttca
    30421 accgagatta ctctcacaga ttcgatccaa caacaagaaa tgtagaacag aattataaaa
    30481 gaacagaaac actgaggata agtcagttac tctgttcttt tttcaatcac tcatccctcg
    30541 ttaccccctc ccggtatttt agcgaatttt tcttattttc catctgtttt agatagctgt
    30601 ggataactat aatgtttgtg ttaaactatt ttttccacag ggaagaaaaa actattttta
    30661 accacgttgt gtataagttt ttatagttcg tttttttaaa cacatttacc cttatttcaa
    30721 tccatgttat ttggggatac ttatctctaa aacataaata gaataacaga catgtctact
    30781 ggttttgttt tattcgagga attattcaat aatagctagc aaaatattaa aaataaccgc
    30841 tctatcttcc catcaattta ttttgcattc aaaaaatgat ctatctttac ttttgttgat
    30901 tgacttctct tggaagatga tcgttccgat accttaattg gatacgaacc gatgttgtta
    30961 agatcctttc ccacttttct tctagttaga caaattattt tagacaaacc aaaaaagagg
    31021 ttgtgacaat aatttgtcac aacctctttt ttaaaatatc tattttgttt gattgttcat
    31081 tgcgtcttct gcagccattt gcgcttcaat atctgaaata ctgtagacat tttcgcttgc
    31141 tacaccgact tcttttggtt ccatgtcacg ataacgttgc ataccagtac cagctgggat
    31201 gatcttacct ataataacat tttctttcaa accaagaaga ggatctttct taccgcggat
    31261 cgcagcatcc gtcaagacac gagttgtttc ttggaatgat gcagcagata agaaactatt
    31321 tgtttctaat gatgcttttg tgatacctag taagacaggt cgagaagttg caggaacgcc
    31381 tcctgagact agcgtattgt agttttgatc tttaaagtct gcgatgtcta ataatgtacc
    31441 tggtaagata tctgtatcac ctggatccat tacacgaact ttacgcagca tttgacgaac
    31501 cattacttcg atatgcttgt cgccgatttc taccccttgc atacggtaaa cacgttgtac
    31561 ttcacgaagc aagtagtttt caacagataa tacatcgcga acttgcaaca attgttttgg
    31621 atcgattgat ccttctgtca atggtgcacc acggtggatc atatcgcctt ctgctacttt
    31681 catacgtgct gtataaggaa cagtatacgt acgtgtatct gtttttcctt tgattgttac
    31741 ttctttggta cgtgttgcag gatcttctga gatatcgatg acatcccctg ttacttctgt
    31801 gatcactgct tgccctttcg gattacgtgc ttcaaagatt tcttggacac gaggaagacc
    31861 ttgagtgata tcgtctccgg caaccccacc tgtatggaat gtacgcatcg tcaactgagt
    31921 accaggttca ccgattgatt gagcggcgat cgtaccaact gcttctccga cttcaacgtc
    31981 agagccagtt gcaaggttgc gtccgtaaca atgtttgcag acaccatgtt ttgtattaca
    32041 tgtgaagaca gaacggattg tcactgtttc gacacctgca tcaacgattt gttttgcgat
    32101 gtcttcggaa atcaattggt ttcctgcaat gatcaattca tttgtttcag gatgacgtac
    32161 ttctttacgg gtgtaacgtc ccactaaacg atcttctaat gattcgatga tttcatttcc
    32221 ctcgcggatg gcttggattt caagacctcg gtcagtaccg caatcttctt cacggatgat
    32281 tacatcttgg gccacgtcaa ccagacgacg agtcaagtaa cctgaatcgg cagtcttcaa
    32341 ggctgtatcg gtcatccctt tacgagcccc gtgagttgag ataaacattt ccaagacgga
    32401 tagtccttca cggaagttcg aaatgattgg caattccatg attcgaccat taggagcggc
    32461 catcaaacca cgcattccgg caagctgtgt aaagttggaa atgttaccac gagctccaga
    32521 atcagacatc atgaagattg gatttttcgc ttccaaacct tccatcagtt tttgttggat
    32581 gctgtctttt gcagcattcc aaacagcaat gacacgttca taacgttcgt catctgtgat
    32641 cagaccacga cggaattgtt ttgtaatagt ttcaacttgt ttatgggcat tttcaatgat
    32701 ttcttgtttt tcattcagta cactgatgtc agcgatccct actgtgatac cggcatgtgt
    32761 cgaatgtttg taccctaagt ctttcatacg gtcaagcatc ttagaagttt ctgttacttt
    32821 gaatcgtttg aagacttctg cgatgatgtt tcctaagttt ttcttcttga acggtaatac
    32881 taattcttgc tctttgatat gtgcaggaat atcagtacca gcttcaacaa agtatttgtc
    32941 cggtgtttgg acggtcaagt tgaagtctgt tggttcattc agataaggga attcaggagg
    33001 catgatctcg ttgaagatga tcttaccaac tgttgtgatc atcgtacgtt ctttttgcca
    33061 ttcagtaaat ggtttttcac ctaatgagtt agggttcaca ccgatacgtg aatgtaaatg
    33121 aacatatccg ttgcgccatg cgataacggc ttcgttttga tctctgaaga ccattccttc
    33181 gccttcgcgt ccttcttctt ccattgtcaa gtagtagtta cctagaacca tatcttggga
    33241 aggagtaaca actggtttac catctttagg gttcaagatg ttttgagcag ctaacatcaa
    33301 catacgtgct tctgcttgtg cttcttcatt caatggaacg tggacagcca tttggtctcc
    33361 atcgaaatcg gcattataag cttcacatac aagtggatgc aaacggattg cgcgtccttg
    33421 tactaaaact ggttcgaatg cttggatacc aagtcggtga agcgtaggtg cccggttcag
    33481 caatactggg tgttccttga ttacgtcttc taaaacatcc catacttcgt cttcttgacg
    33541 ttcgatttta cgtttcgcat ttttgatgtt tgaagctagt tcgcgttgaa caagctcacg
    33601 catcacgaat ggtttgaata attcaatggc catttctttt ggaagtccac attggtacat
    33661 cttcaagaaa ggtccaacaa cgataacgga acgacctgag tagtcgacac gtttacctag
    33721 caagttttga cggaaacgac cttgtttccc tttcaacata tgagaaagag atttcaatgg
    33781 gcggttacct ggtccagtaa ctggacgtcc gcgacgaccg ttatcgatca atgcatctac
    33841 tgcttcttgc agcatacgtt tttcattttg aacgatgatt ccaggtgcat tcaagtctaa
    33901 caaacgtttc aaacggttat tacggttgat tacacgacgg taaagatcat tcaagtcact
    33961 tgttgcaaaa cgtccacctt ctaattgaac cattgggcgt aaatctggtg gaataacagg
    34021 gataacgtcc ataaccatcc agcttggttg atttcctgaa gcgcggaatg cttctaaaat
    34081 atccaaacgg cggattgcac gtgtacgttt ttgtccttgt gcagatttca attcttcttt
    34141 caattcggct gcttctgcat ctagatcaac attatcaagt aattgtttaa tcgcttctgc
    34201 acccatagca gcttggaatt cttgtccata ttgttcacgt ttttcgcgat attcgcgttc
    34261 tgtcaacaat tgttttttct ctaaattcgt atttcctggt tcgatgacta catatgaagc
    34321 aaaataaatg atttcttcca aagcgcgtgg gctcatgtct aatacaagac ccatacgtga
    34381 aggaatccct ttaaagtacc agatatgtga aacaggagct gctaattcaa tatgtcccat
    34441 gcgttcacgg cgaactttcg aacgagtaac ttccactccg catcgatcac aaacgattcc
    34501 tttataacgg atacgtttgt attttccgca ggcacattcc cagtcttttg taggaccaaa
    34561 aatccgctca cagaacaacc cttcgcgttc aggttttaag gtacgatagt taatcgtctc
    34621 aggttttttt acttcaccgt aagaccagct tctgattttt tcgggagaag ccaaacctat
    34681 ttgcatactt tcgaatttat ttacatcgat caaaaggggt tccctccatt tcatttaatg
    34741 gcaaaccgca agacggctga tcttcgaccg ccttgcagct catagaccgg attagtctaa
    34801 attatcttct gcggtttcaa agttttggat gacatcgtct ttttcatctt ccacttgttc
    34861 agcagctttc ttttctaatt ctttggcagt ttgctgttcg gcaaattttg tcaaagcatc
    34921 aacagtgatc aaatcgtcgt cttcatcgtc catgtcgcga agttcgatct ctgtttcttc
    34981 gatatccaag acacgcatgt ccagacctag tgattgcaat tctttcacca atacgcggaa
    35041 tgattccggt acacctggtt ttggaattgg ttcacccttg acgattgctt cgtatgtttt
    35101 cacacgtccg actacgtcat ctgatttgta tgtcaagatt tcttgtaaag tataagccgc
    35161 accgtaagct tccagtgccc atacttccat ttcaccaaaa cgttgtccac caaattgagc
    35221 tttacctccc aatggttgtt gtgtaaccaa tgagtaaggt ccgattgaac gtgcatgcaa
    35281 tttgtcatcg accatgtgag caagtttgat catgtacatc acaccgacag acacacgtcc
    35341 atcaaatggt tcacctgtac gtccgtcgta cagaacagtt ttcgcatcgc tggccatacc
    35401 ggcttcacga actgtttccc atacgtcttc atcacttgca ccatcaaata ctggcgttgc
    35461 tacgtggata cctaactgac gtgcagccat acccaagtgt aattccaata cttgcccgat
    35521 gttcatacgt gaaggcaccc ctaaaggatt cagcatgata tcgattggtg ttccatcagg
    35581 taggaatggc atatcttctt ccggcataat acgggataca acccctttat ttccgtgacg
    35641 accggccatt ttatcccctt catggatttt acgtttttga acgatataaa cacgaactaa
    35701 catgtttacg cctggtgaca attcatctcc agcttcacga gtaaagattt tcacatcgtg
    35761 aacgataccg ccgccgccat gtggtacacg taatgatgta tcgcggactt cacgagcttt
    35821 ttcgccaaag atcgcatgca atagacgttc ttctgcagat aattctgtta ccccttttgg
    35881 cgtgactttc cctacaagca aatcgccatc ttggacttct gcaccaatac ggatgatacc
    35941 catttcgtct aaatctttta atgcgtcttc cccgacatta gggatttcgc gtgtgatttc
    36001 ttcaggtcct aattttgtat cgcgggcttc tgattcatat tcttcaatat ggatagaagt
    36061 gtatacgtca tcttttacca aacgacggct catgatgatc gcatcttcat agttgtaccc
    36121 ttcccaagtc atgaaaccaa ctaaaacgtt ttggcctaga gccatttcgc cttgttccat
    36181 agatggtccg tctgccagag tatccccttt ttcgactttc tcacccaaat gaacgattgg
    36241 acgttggttg tagcttgttc cggagtttga acgacggaat tttgttacat cgtatttatc
    36301 taatgcaccg ttgtcgcggc gtacgcggat ttcagaagca tcaacgtatt caacgacacc
    36361 gtcatgttta cataacaaag cagctcctga gtcatgcgct gatttgtatt ccataccagt
    36421 accaacccat ggtgcttgag gattgatcaa cggcactgct tgacgctgca tgttggcacc
    36481 catcaaggca cggttggagt catcgttttc caagaaagga atacatgctg tcgcaacggc
    36541 aactacttgt ttaggtgata cgtccatgta gtctacttta tcaatagata cttctaagtt
    36601 ttcactttga gcacgagcca taacaacgtc ttgagcaaat gttccatctt cattcaatgg
    36661 gctgtttgct tgggctacga tgtaatggtc ttcgatatca gctgtcaagt agtcgatttg
    36721 gtcagtaaca cgacctgttt cacggtctac acgacgataa ggtgtttcga taaatccgaa
    36781 tttatttact ttcgcataac tggacaaact attgatcaac ccgatgtttg ggccttcagg
    36841 agtttcgatc ggacacatac ggccatagtg ggagtagtga acgtcacgaa cttcataacc
    36901 ggcgcggtca cgagtcaatc cgccaggtcc taaggctgaa agacgacgtt tgtgtgtcaa
    36961 ttcgccaaga gggtttgttt gatccatgaa ctgtgacaac tgagaagaac caaagaattc
    37021 tttgatactt gccacaactg gacggatatt gatcaattgt tgaggtgtca atgtttcagt
    37081 atcttggatc gacattcttt cacgaaccac acgttccata cgtgctagcc cgatacggaa
    37141 ttggttttgt aaaagttcac cgactgaacg gatacgacga tttcctaagt ggtcgatatc
    37201 atcgacattg ccgatacctt ccatcaagtt caagaaatag ctcattgaag caatgatgtc
    37261 ggctggacga accgttttta cagctgcttc tggatagccg tttccgatca cgtttacttc
    37321 acgttcaggg tctcttggtg aaaatacttt gatgacttgg acagtcattg gatctgtgac
    37381 cacaccgtct tctgatggat agtaagtcac tgaatttagg ccattatcaa tgaaaggagc
    37441 tagtgtttcc atgacttggt gagtcagaac tgttcctttt tcaacgatga tctcgcctgt
    37501 ttctggatca actaatgttt cagctaatgt cagattcaat agacgtgttt tcaaatctaa
    37561 ttttttgttt actttgtaac gaccaacgtt tgctaagtcg taacgttttg gatcgaagaa
    37621 acgagcattc agcaagttac gtgagctgtc tgctgttttt ggttcgcctg gacgcaaacg
    37681 ttcgtaaacg tctttcaaac cttcttctgt tcttgagtcg cttgcgtttt tatgcaagtc
    37741 tttttcgact gtattgcgta atgtttcgct gtcgccgaag atttcaaaga tcgtatcatc
    37801 ggaaccaaat cctaacgcac gaactaatac ggttaaagga atcttacgtg tacggtcgat
    37861 acgtacataa gagatgtctt ttgcatcagt ttccatttct agccaagctc cacggttagg
    37921 gataacagta gaaccgaatc cttcttttcc gtttttgtcg acttttccat ggaaatagac
    37981 acctggtgaa cggactaact gagaaacgat tactcgctct gccccgttga tgatgaatgt
    38041 tccttgttct gtcattaatg ggaaatcacc gaagaatact tcttgtgctt tgatttcgcc
    38101 agtctcgcgg tttgtcaaac gcaatgttac atgtaatggt gcagagtagt ttgcatcatg
    38161 cgcacgagct tctgctactg tatactttgg ttctttcaat tcataatcta caaattccaa
    38221 agatagattg ccattgaagt catcgattgg caaaatgtct tcaaacattt ctctaagtcc
    38281 ctcatctaag aaccattggt aagagtctgt ttgaatttcg atcaaatttg gtaattccaa
    38341 cacttcactg attcttgcga aacttctacg ttcgcgatgt tttccgtatt ttactacgtg
    38401 tccagccaag ctcttcaccc ctctaaaatg tcattcaaaa agactacccg atcaattgtt
    38461 acaataatat ttcaaatatt atttttaggt aacaacagac attttttgcg gcttttaggc
    38521 aaaaaaaaac caaaaataga acattctctt gaaaccttac ttcgtgagct tctacttttg
    38581 tttccttata ttaaagccga aacggacaat atttcgtttc tgccttttct tcatcagata
    38641 gtttttgcaa cttcatatac tacacttatt ttctataaaa gtcaacactt cctggcgttt
    38701 cttctgcttt ttcgcaacat tttcttaatg ttttcaattt tatgttttca attatctgac
    38761 aattcttaat tgacagacct ttttatctcg gttatacttt agaaaattca ttttgtggag
    38821 gaacttctat gcgaaccaac atcaaccaaa tcgcaccaac acaactgaat aacttttact
    38881 ttagctactt tagataggtt tgtattcatc atgatggata caaacgttaa acgacagttt
    38941 gtatctatga tggctaaaga actttgtatt gcagcaaaaa gccacgtaga caaaatgcta
    39001 agtggctgag cagtgaaaat acaaagtaaa agtatcaagc attctacttt ttcaatttat
    39061 tttccgactt ttcaatccat ttggacattt tgtctgaatg gattttttgt ttcccaaaat
    39121 taaggaggaa aaagaaatga aaaaaaatat aatcatcgga ttaggcgctg ccctgctact
    39181 agctttagga ggttgcgcac aaaaagacaa ttccgcagag gcatcagatg gacaaaaaac
    39241 aattggcgta ttgcaacttg tacaacataa ctcgttagat gctgcctata aaggattcaa
    39301 agaaggatta gcagaagaag ggtataaaga aggcgataat ctaacaatcg attatcaaaa
    39361 tgcccaaaac aatcaagaca acctgaaaag tatgagtgag aaactcgtca aagaagatcc
    39421 tgatttgcta cttggaatcg ccactccagc agcacaatct ttattaaatg aaacaaccac
    39481 tatcccgatg actgtcacag cggtcacaga cttggaacaa gcccatttag tcacatccaa
    39541 tgaaaaacct ggcggcaatg tgactggcac gacagatatg gtgccaatcg aaaaacaaat
    39601 cggcttactg ttaaatatcg tgccagacgc caaaacaatc ggcatcatgt ataacaacgg
    39661 tgaagcaaac tctaagatcc aagctgattt ggcagaaaaa gaattgaaaa aagcgggcgt
    39721 caaagtcaaa gtactgacag caaattccac aaatgatgtc caacaagtga cgaaaagtct
    39781 agctaaagat gtagatggaa tctatattcc tactgataac acgttcgcaa gtgcggcaag
    39841 tgttgtcggt gaagtcgcaa aagaaacaaa aacacctgtc gttgctggcg cagttgatca
    39901 agtcaaagac ggcggtctag caacagtagg aatcgactac gaaagcttgg gtaaacaaac
    39961 agggatcatg gcagcaaaaa tcttagacgg caagaatcca agtgaacttc ctgtcgaaac
    40021 agcgaatgat ttggaactgt atgtcaataa agacatggcc aaagcattag gcatcaatcc
    40081 agacagcatc aaagaagctg aataaaacac aggaggattc tcatgacaaa tttgctctct
    40141 cttttcttat catctgcatc gcaaggggtc ttatgggccc tacttgcgat tggtgtatac
    40201 gtcactttcc gtatcttgga cattgccgac ttgactgctg aaggaagctt tcctttaggt
    40261 gcagggatcg cggcagtaag tattacaaat ggctatcatc cattgacagc ttgtcttcta
    40321 ggctttttag ggggtgcggc agcagggctt gtttccggac tgcttcacac aaaattgaaa
    40381 attcccgccc ttcttgcagg gatcatcacc atgactggtt tatattctgt cacatcccgt
    40441 gtgatgggtg cgccgaacat ctctttattg ggtcagccaa cgatctttca ttgggtcgag
    40501 tcattcggca taacaaaaga caacgctgta ctgatcgtcg gtctgctttt tgctttgatc
    40561 gttgtatgtt tacttgtttt gttcttacgt acagaaacag gacttgctat tcgtgcaact
    40621 ggcgataatc tggcaatgag cgaagcaaac gggatcaaca cagataatat gaagatcatc
    40681 ggttatatga tttctaatgg atgcattgct ctttctggct ccttgcttgc gcaaaacaac
    40741 ggctttgctg atttaaattc tggtattgga acgatcgtaa ttggacttgc ttctattatc
    40801 atcgcagaag ttttgttacg taaccagcca ctatttctcc gtctcttgac tgtcgtttta
    40861 ggtgcggtca tttatcgttt tattttagcg ttagtcttcg aactgaatgt tgaaccaagt
    40921 gattcgaaac ttgcttctgc tttcgtgcta gtgatctgtc tttctttccc gcagatccag
    40981 caaaaattcc atttacctca aaaaaagaaa ggcggtagtg tcaaatgaat gacgtcttaa
    41041 cattggaaaa tctccatcat acctttgaaa aaggaacgat caacgaaaat catgtcctga
    41101 aaggcattaa tctttcctta gatcccggcg acttcgtgac gatcatcggt ggaaatgggg
    41161 ctggtaaatc tactttgctg aacagcatcg ccggtacatt ttctgtcgat cagggtcaaa
    41221 ttttattgaa tggaaaaaat atcacgaaaa aatctgttgt cgaacgttcg aaatccatca
    41281 gtcgcgtatt ccaagatcct aaactaggga cagccgttcg tttaacggta gaagaaaatc
    41341 ttgctcttgc tatgaaacga ggaaaaaaac gcggcttctt ccgtggtgtc aaaccacaag
    41401 accgaacatt tttcaaagac cagctggttc ggctaaattt ggggttggaa aatcgtttat
    41461 cctcagaaat cggtttgctg tctggaggac aacgtcaagc tattacgtta ttgatggcca
    41521 cattacagcg tccagaactg atcttattag atgaacacac tgcagcgctt gaccctgcta
    41581 cttccaaaac ggtgatggaa ttgacagaaa agctgatcac tgagcagaat ctcactgctt
    41641 ttatggtcac tcatgacatg gaagacgcga tccgctatgg gagtcgactg atcatgctgc
    41701 atcaaggaag gatcgtcgta gatatccagc aagaggaaaa acagcagctc acagtgcccc
    41761 aattattagc gttgttcaaa caaaacagcg gcacggcatt aaaagacgat caagtacttt
    41821 tatcttcata atcagaaaga ggatgggaca aaaaatgaac gcccaccctc ttcttttttc
    41881 tctttccttc tttcgttttt gctttatgta tcaaccgttg tttcttcgaa aactattttg
    41941 ttctctcctt cttcctgata gaatggatgg gaaaggagct tgatgccatg acaacgaaag
    42001 acaaggtact tcaattattg aaaaagacac cagattttct ttccggagaa aagttggcac
    42061 agcaactgga agtttcccgg acaagtattt ggaaagccat caaagaattg gaaaaggcag
    42121 gctatcgttt tgagcacggg cctacagggt atcgttacct gccctctgac gtactagatg
    42181 caaaagaaat ccagcaagag ctacggtcgt ttattcctga tttgaaagta caaacgatgt
    42241 tgacttctga atctacgatg aaagatgtca aactggcagc agctcaagga gattctgttt
    42301 cgaagttatt tgttgctgat acacaagaac aagctcgtgg acgttttgga cggccttttt
    42361 tcgctgaacc tggacgagga atctacatga gtctcttgct ccatccagac aaacggtttg
    42421 atgaattgcc tcaatatacg atcatcgcag ccagtgcctg tgtgaaagcc attgaatctg
    42481 taaccggtcg ttctgcttcg atcaaatggg tcaatgatat ctacatggat ggaaaaaaag
    42541 tctgtgggat tttatctgaa gcagtcagtg atatggaaag cggcaggatc agtcatatca
    42601 tcattgggat cgggcttaat ttttctgttc ctcaaaatca ttttccagct gatctccaag
    42661 aaaaagccat ctctgtcttt cccgacggac aagctagtgt cacaaggaat cagctaatca
    42721 gcaaaatctg gcaagaattg ttcacttatc tttctcaccc agaaaaagcg ttggacttct
    42781 ataaagaaaa atcattcgta ttgggaaaaa ccgtgtcgtt caatcaaaaa ggaacgactt
    42841 atgtcggtct tgcaaaagaa atcggtgatc atggcgaact gatcgtccaa acaaatgaac
    42901 agaagatgag tttgtcttct ggcgaaatca gtctcacatc tatccgtacg caataattat
    42961 cagatttcac aaaaggacac ctgatctcat acagtgtcct tttttctgaa ttctcttcaa
    43021 aagactctcc ttattctatc agctgacagt ttctctatcc tctagaaaaa ggtattctaa
    43081 aaatatctat aaagcatggt acactttctt taaatgaatc aatgcaaaaa acaagagagt
    43141 catcagttta cacgatcaaa agagatagca ggccgcttcc tttttcaatc ttatagatag
    43201 accatgggta acgataaaaa caaaaaagaa cttgcgactt attttgccgt aagttctttt
    43261 ttagctagtg ccttaatctc ttaatcccaa caaacgtctc gcatagatca cctgaccaga
    43321 gtgcatagca gcatcatcaa taatagaaac gagacgcatt cctcgtgtga ctggtggtgt
    43381 ccagttctcg tcaattactt catctagctc ggaaggttcc agccttctta agtaagaaat
    43441 agcaaaatca gcagctcctc gcaagtatcc tagcagatct tctatttgat tagcttggat
    43501 ctgctttgct tgttccagac tatgcttcca atcttgttcg ttatctgcta tcgtcatcgg
    43561 aaaccggctt tcccagtctt gactatgcca aattggagaa gttcctgcta aatcagcaat
    43621 ctggaaatct aattctcgag cggtatgcca agccagccaa gtcattgatt tgatctgcgg
    43681 ggctctatct tccactggaa aacgattggc ctcctcaata gagacgcctt caaacaaacg
    43741 ttcaaaccga tcgtatgccc gatccacact ctcgcatagc atatctaaat aatccattta
    43801 ttttcctcct ttttatttat cctacttact ttttcctaat tctaggatac ctacttatat
    43861 gttactacac acccttattt tttacacttt aacagtcacc atcatcaata gtttggagat
    43921 tgaaagaaaa agatagtgtc gaactatcaa ttgaacaaga agcaacaata tgaacgtttc
    43981 attaatattc tttaaataaa caatgatttt tcaaacgaac agtttaagac taggagaaaa
    44041 agagtataat aggaaaatgt ttgaaaggaa aggaagggga aaaatgaaaa aaagggcaag
    44101 tttactagtc ctagttgctt tgttgttcaa tatcttttcg tatggtgtgg ccgtttcagc
    44161 ggaaacatac attcctaaag accctgggaa tatttataat gatctaactg gtaactatgg
    44221 cttgttagga attgctagtc aattccacgt atttgctaaa aataagacaa ctgtgaatgc
    44281 acatacagac ggaaatatag caacacatga acttgatgca aaaaataatt ttggtacaga
    44341 gcttcatgaa ggtttattga accaagaaat aaattatata caaaaaactg attctttcgc
    44401 taattcttca ggcataccta caggtagcac aagaatgaat aaatttgtcg ttggcagtaa
    44461 cgtggatgta gcacttcaag atggtcatcc tacagtcaat ggatccaggc ttgatcactt
    44521 aactccagat gatttttata aagatcgtgc tgggcatgaa tatattgatt tcgagcagga
    44581 atttgctaaa ttgaatgtag cagcaaatga tcttgcaacg atcactccag ctaaaacata
    44641 tactgctgca gattttccag acatgaacaa tcggacaatc gatttaactg atttatcaga
    44701 taccgggttt ttattggtaa atatcgatgc agaagtgtta acgatgaata cgccacttca
    44761 aatcattaat ccaaatgatc aagtagttgt atttaatgtc atcaacagtt cttctgcatt
    44821 gaacgtacag tcaccaatca aatacaatga tcgttcaaat catgaaacag aagatttttc
    44881 ggatgcgaat atctcttgga attttggaaa tgagatgact gatttgacaa tcagtgctcc
    44941 ttttcaagga acaatccttg cgcctaatgc tactatccgc attactcaaa accaagacgg
    45001 aacgattata ggaacaaatg tgattttaga tgcagctacc aatcgttggg atcctaacga
    45061 aatatttatc agcgacaatg gtacagatac gacagacaca agcgacagta cagatacatc
    45121 taccacaagt gatagctcag attcatcaac ttcaagcgac agtacagata ctacctctac
    45181 cacaagtgat agcacggata catcagcttc aagcgacagt acagatacta cctctaccac
    45241 aagtgatagc acggacacat cagcttcaag tgacagtaca gatactacct ctaccacaag
    45301 tgacagcacg gacacgtcag cttcaagcga cagtacagat actacctcta ccacaagtga
    45361 tagcacggac acatcagctt caagtgacag tacagatact acctctacca caagtgatag
    45421 cacggacacg tcagcttcaa gcgacagtac ggatacatct accacaacaa gtgatagcac
    45481 ggacacgtca gcttcaagtg acagtacaga tactacctct accacaagtg atagctcaga
    45541 ttcatctacc actacaagtg atagcacgga cacgtcagct tcaagtgaca gtacagatac
    45601 atcctctacc acaagtgata gctcagattc atcaacttca agcgatagta cagacacgtc
    45661 gtctaccaca agcgatacac gtcatgcaag tgtctccttc tcaaaaacaa gttcggataa
    45721 caggacgaac tttaacttaa ctaataaaac aagcaatgac aatggaaaca aatcaaaaag
    45781 agcacttcct aaaactggaa gtcaatcgaa taattggata acactcgcag gcgttatatt
    45841 acttgtaatc ggtttaagaa tcacttttat gagttataag aaatcgaaaa ggtaaaaaaa
    45901 tattcaagat aaaaaagagc aacgtcgcta acggacgttg ctctttattt ttgcttttta
    45961 tcataaatac aagaactaca attcaatttg tcaaaaaaca cgtagatttc cttctgagaa
    46021 atttatttac gtatcgaacc tttccctatt cagtctttgc gatccaaaat cagttgggta
    46081 agggaaaaga gattctcttt tgttcgaaga gaggtggcag gtaatttctt tatttccttc
    46141 aaagcctttt ctgtgtaaga ggccgctaac ttcctagctt cttccacgcc tcctgtatgt
    46201 tcaacgattt tgcgtacggc gtcgacatct tcttctgtca ttgccgcttt cttctctaaa
    46261 taaggtagta gctttggttt gtttttttgc aaagagtaga tcaacggtaa agaatagacc
    46321 ccttgacgca tgtcttctag tacaggtttg ccgatttcct ggctctcttt tgtataatct
    46381 aaaatatcat cgataatctg aaaggccatc ccgatagaaa gtccgatatc tcctgctttt
    46441 tttgcgaagc gctcgcttgt tccactttca aaagcgccaa cagaacaact taattggaac
    46501 agttcagctg tcttacctga aatattttcc aaatattctt ttacagaaag ttcataatta
    46561 tagcgatcat ccatctgccc taattcgcca ttcaatactt tttccatact gcgagaattc
    46621 aattggagac ttttcaagga agtggagtaa tcagacaata gtttaaaaca gcagacaaat
    46681 aaatagtctc ctgcatagac agaaacgaca ttaccaaact gactacggat attaggctga
    46741 ccacgtcgca gatccgcttc atcaacgata tcatcatgga tcagtgtggc cgtatggagc
    46801 atttcgatag aagctgccaa agcgatggct ttgtctcgat cttgctctgg tccaaattgt
    46861 gagaacaata gctgataggc aggacgcaac agctttccgc cggcatggat cgtttctttg
    46921 attgcttgtt cgacaggttt gtttttcagg cggacagttt cttccatccg ttttagtgtt
    46981 tctgtcaatt ctttacttaa agcaggatag ttttcccaca ttggatgtag cttcataaaa
    47041 aaactccttg tgtgacgatt attttttggt aaacattggg ctccattttt gtttggttcc
    47101 ctgctcataa tctttttgga atcgttgaag tgtctgcaca tgtcgtaaaa gataattcgc
    47161 tgcgatcccg attgcaatcc ctgataaaat ccctaaaact gaaaggactg gcaagtacaa
    47221 catgactgtc catgattgtg caatgaaaga agccatcaaa agctgtccga cattatgcat
    47281 gaaaccgccg gccgcactaa ttccgatgat actgacacgt tttggtccta attgcttgac
    47341 caataacatt ccaaaataac taagcaaagc accactcata ctataaagga atgtggaaat
    47401 cgttccgcct agcaaagtag tcaggaatag tcgtagccaa agtagtaaga agctgtctcg
    47461 ttttttcatg gtaaaaatgg caacgatcgt gattagattg gctaagccta gttttgcacc
    47521 aggcgcaaaa gaaaaaggat aagggatcat attttccaat aaaccaatga tcactccttg
    47581 tgcaacaagt agcgaaatat aaatcatttt ttgtaattta ctcatggtgt gtgaaggtct
    47641 aataaatcag actgtcttcg tcacctccat ctgttgaacg gacttcgatc actaatttat
    47701 gtggcaaaca gacgatggtt tcgccatttt tcgttgccca gcctcgacgt acgcagatct
    47761 ggtcatcgca atctgcttct tttatccgta ctcgatcgcc atctatctcg atcaagttat
    47821 aatctccgtg ttcatctttg taagtatatg tgttgctctc ttctccagcc atcaaaggga
    47881 atgttttgat ttctgtgcca tccacacgca gtaccgcttc tttttctgct gttgcggtgt
    47941 tttgctgaag accaaaaatc atgactggta aaaaagaaag aacagtcaaa agcaaaataa
    48001 taaaaacatc ccaaggtttg agcagacttt tttcagcaaa ttctttaagg ctcatttttg
    48061 ttctcctttt tccagattaa ctcacccaat tttagcataa aaacaagagg ttgtgacacc
    48121 tgtgtcttgc tttgagtaat aagaacgaag aatagaaaac agctttccat gttttctaat
    48181 tctttgggct tattaccgag aagcagcagg tggaacaccg tttataacaa gaggttgtga
    48241 cagaagtggg caacttcaag aaataagaag gaatttacga aaattacttt tcaagttttt
    48301 gtgaaatttc ggcttatttt tcgaagaagc tacttctgga acaccgttta taacaagagg
    48361 ttgtaacaga ggttgtgaca cctgtgtctt gctcatggag gcttatctcg ggcatcttat
    48421 tcctttaatt ttacataaaa aggaggctgt gacggtttgt ctcagcccct ttctattgaa
    48481 atcttgcatt caaataacgt tctttatcta tttatttctt gccgtactgg gaacttggtc
    48541 taccgtacca ccaattacct agtgtacgtt gtagccatgg aattacccag cgatccaacc
    48601 caatcgcacg tccggaaccg ttcatcaatg cgaaagcaac aaaaatgaac caaatgttta
    48661 cccagtaaaa catgcctgat aagcagaacg tgatcaccaa tacaatcgtt gtcgcactgc
    48721 atagccatgt gaataatcca gcaatcaatg ctaatgccaa ggcaatttca aagaatacca
    48781 tgaatttttg gaagaacatt gctacatcat tgtttggcat gatcacttgc atgatctttg
    48841 tgaaccagtc tggtgacttc tcaatcacag ccattggttc ttcaccgtat gcataactca
    48901 aaccaaaatg tgcagcttga gtcgctgctt ctccggcact tgcccctgct ccgctggcag
    48961 ctgtggttgc atcaccagca gcagaagcac cactggcaac atctgttaca ttgtattccc
    49021 atgtccaagg gaacacactg tttccagtga accatgatcc ttcaccaaac caagtacttg
    49081 gtttgaacac ttcaccattc ccaatgattt ttttggaagc ttcaacgaac caaactaaac
    49141 catagaaaat acgaagtggc acactccata agacattgcc gtaacgagaa gaatgtcctc
    49201 gagtcacgtt gcgatcatct ttgatatgga aaaactcatg cattaaatat tggaacatat
    49261 aataaccaga acgaatatca aagaagtatt tcaaattcac gatatgtttc attacgatag
    49321 ctaaaaatcc gcttaaatgg atcttatcaa ataggttagc taccccccat ttggcaccaa
    49381 tagaaaccat aaatccttga tagtttcctt taaatttatg cttttctccg cctttgatcg
    49441 aagccacgat attcgctgcg gctgtgtgtc ctgtttgttc agcagcttga acgatctgcg
    49501 gtgtcggtgt tcctgggaat tcttcataaa agactaaatc accaatgata tagatgtttt
    49561 tatcttcgta gccttttgcc tgcatatatt cattggcgat caaccgctgt ccgcgtgctg
    49621 attctaatcc aaagtcagca gcgtcagaag ttcctttgac accagctgtc caaatcagtg
    49681 tgtgagtcgg aatgttgcgt ccgtctttca atttgatatg atcagcagcc acttctacga
    49741 ttggtgcatt caataataat tccacgttgt gcttacgcaa atagcgttca gcttttgcag
    49801 catcatttcg atccaacata ttcaagatcg tcggcattgc ctctacgacc atcagtgtga
    49861 tttcgtttgg atctaattta taatctttgg ctaggcgatt tttccagtcg atcagttccc
    49921 caatcatttc aataccggta aatccagaac cacagacaac gaaagtcagc atggctttac
    49981 gcagttcatc atctggttcc aatgcagctt ttgctactgt cttctcaata tgttcccgaa
    50041 tccttacaga atcttcaaat gaccaaaggg tgaatccgtg tgctttgaca ccaggtgtac
    50101 caaagtcatt cggctctccg cccattccaa ggatcagctg atcaaaagga taggaaccta
    50161 atttggtctt gacgattttt cgttctttat cgataccgat tacagtgtct gtgacaagtt
    50221 gtacattctt ttgacgagca aacaaatgct gcagatcgta ttggattgct tctggttcta
    50281 cacgtccgcc agctacttca tgcaactcgg tcatcatagt atggtacgaa tgacgatcga
    50341 tcagagtaat tgctacatct tcttttttca gtttctttgc caagtatttt gtagctgaaa
    50401 caccggcata tcctgctcct acaacgacaa tattttgctt tgccattgat catccgctcc
    50461 ttatagacta tctatataat gaatatatga taattctttt agtttgatac ttattacaca
    50521 atctccatat attatataga tgttctgttc ttttgtctaa ctccttttgg atttttttca
    50581 aattttaaaa taaagcgttt tttcacgaaa tttgctgaaa gaaaacgttt gaaatattac
    50641 atgggttatt tatcaaaaat agttgactta taggctaaaa agattagaat gtataatgaa
    50701 tcacaaagaa aaattgtgga ctagaaatgg aatggtgggg tattaaaatg aagaaaaaac
    50761 gttttgtaac tggatttgcc gtattagcat tttcagctct agtgttaggc gcttgcggcg
    50821 cagatgacaa cggaagctca aactcttctt ctgaatcatc tacagcacaa tcttcgactg
    50881 caaaaagcac aacagaatca tctgcaaaag ttgtagctgg cggagatttg caagacggca
    50941 catataaatt agaagaaaaa aattattcaa atggttaccg tgcagtcttt gaaatggttg
    51001 taaaagacgg caaaatcact gaatcaaaat atgataacgt caatgaaaac ggcgaatcaa
    51061 aaacaaaaga tgcagaatac aacaaaaaca tggaagcaaa atctggcaca aacccagaaa
    51121 aatacattcc tgagttgaat gaccaattct taaaagcaca aagcgcaagc ggtgtggaag
    51181 tcgtaactgg agctactcac tcatctgaaa gcttccaaaa ctacgcacaa caattgatcc
    51241 aagctgctca agctggtaac acagatacaa tcgaaatcga caatggtgct gatttgaaag
    51301 acggcactta caagcttgaa gaaaagaact actctaacgg ctaccgtgtg caattcgaaa
    51361 tgacagttgc tggcggcaaa gtaactgaat caaactttga ttatatcgac aaagacggta
    51421 aatcaaaaca agatgataca gaatataatg aaaatatgaa agcaaaaagc ggtacagaac
    51481 caaaaactta catccctact ctgaacgacg aattcgtcaa agcaatgggc gaagaagatg
    51541 gctcacctgc taatgtagaa gttgttacag gtgctacaca cagctcacat tcattcatca
    51601 tgtacgcaca acaattagtg aatgcagctg aaaaaggcga cactcaaaca atcgaagtag
    51661 ataatatcgt aacagaaaaa taatcacata atcatactaa taaaagttgg agggtgggac
    51721 gatagccaga ttaggcagtc gtctcacctt tcaaaatgaa gagaggaaaa agatgcggaa
    51781 aaaactacta ttttccttaa tcacactggg catctctttt atggtgctaa ctggctgcag
    51841 caactcagca aataaaacaa ccactgaaag ttcagcagct accaagatat tgaaagatcc
    51901 atattctgat gaacagttct tgttagggac ttacgttcgt atccgtgtgt atgatgaagg
    51961 caaagaatca gcattgaaac ctgccttcga tcgagtaaaa gaacttggtg ataaaatcac
    52021 gatcaatcaa aaaggttctg agatcgatga agtgaatgag caagctggga tcaaaccagt
    52081 aaaagtttct gatgacgtct acacgttagt caagcgggcc tacgaatata gccaagattc
    52141 tcaaggcgga ttcgatatgg cgattggtgc aatcacacaa ttatggcgaa tcggttttga
    52201 tgacgccaga aaaccttccc aagaagaaat cgatcaggca ttgaaattag tggattatca
    52261 taagatcgaa ctaaatgaca aagaaaaaac tgtttatcta aaagaaaaag gaatgatcat
    52321 cgatttaggt gcaatcgcca aaggttacat tacggacgaa gtagtgaaag tcttgaaaaa
    52381 acaaggagtg accactgcca tcgttgactt gggcggtaat gtctatgtct taggccacag
    52441 cccaagaggc gaaaaccaag actggacagt cggcatccaa gatccgaaca tggctcgcgg
    52501 atctgtttta ggttctatta aagaacggaa caaaacatta gttacctctg gaatttacga
    52561 gcgttattta gaagtagatg gcaaaaaata ccatcatttg tttgatccaa aaacgggtta
    52621 tccgttcgac aatgatatcg caagtgtcac gatcataaca gataaatcaa ttgatggtga
    52681 cgggttgtct acagcagtct tttcaatggg tgtgaaaaaa ggactggaat acgtcgaaag
    52741 cgaattgaat aacgggacgg aagcgatctt cgtgacaaaa gacgataaag tatatgtgac
    52801 cgatccaatc aaagacacct tcaaattatc ggaggactcc cattacacga tgggtgaccg
    52861 ttctgaacta aaataaggag tacgaaccat gacactaaaa atctttttag aagtagttga
    52921 aatacgaaca aaggtggcta gtgtatttcc ttttgcggta ggggtattat tctctatcgc
    52981 tttttttcat gagatacatt ggctaaacac ttttttattt tttattggaa tgctagtttt
    53041 tgatttagcg actaccgcta tcaataatta catggattat gaaaaggctc attctgaagt
    53101 atataagtat gaagaaaatg tcattggccg cagaaatcta tctcctgtat tgattcgaaa
    53161 catgatttta gctatgattg catttgtttt tgttatcggc gttattctaa cgatcaatac
    53221 gggttggctt ttacttttga tgggatttat ctgttgtttt attggagtat tctatacatt
    53281 cggaccgatt cctttatctc gaatgccttt aggggaaatt ttcagtgggg tgactatggg
    53341 ccttggaatt tttgccatgg tcgtttatgt caatacattc ggttcagatg tctatgatct
    53401 gacagtcaac ttttcatctg gacaatttgc tttggtagga aatctttggg ctatagcagc
    53461 aatcgtatta gcttccttac ccttggtgtt tacgattgcc aatatcatgt tggctaataa
    53521 tttgcgggat cttgaccgtg atattgaaaa tcatcgctat acactagttt tttatatcgg
    53581 acgtcctgcg ggaacagtct tattccagct attgatgtat gcttgctatt tcgtgatttt
    53641 aatggggttg ttgagcggta tctatgaatg gccgatcttg gtcaccttct tgaccttgcc
    53701 aattgtgaag aagcatttgg atcaattcaa gcaaacgtta ccccagccta tcagtttcag
    53761 ctattccatc aaaaatatgg tcttattcaa tgccagttat gtattggggc tattactaac
    53821 aatcattatg caagccattt aacagcatga agaggttgtg aaaaaactgc cctgcatcaa
    53881 gtaataagaa cagccaaata aaaatagctc cttatatttt tcgtttggtt gggcttatta
    53941 ccgcagatgc aagcttttga acaccgttta tcaggaggtt gtgaaaaagt cgcttagttt
    54001 cgagtattaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttggctta
    54061 ataccgagaa actggctttt gaacaccgtt tatcagaggt tgtgaaaaag tcgcttagtt
    54121 tcgagtatta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt
    54181 aataccgaga aactggcttt tgaacaccgt ttatcagagg ttgtaaaatc agtgttttgt
    54241 cctgagtatt agagaaacaa atggaaaaat agttcctcat attttgccgt tttttgagct
    54301 aataccgaag gacagctgat tgaacactat ttgttcggaa aaagtgacta actatgacac
    54361 aatttttttc acagtccctt ttttcacttg atttgattcg taccagcgaa cttgacgaga
    54421 actttcttgg tttggtaact atatggtatt cttttaacat cctcgtttgc ttcttctaat
    54481 ttatttatac aaagtcacac ttttttaaac atctgttctt cgatacattt ctatttcttt
    54541 taaacatcga taccaatatt ttgaacttaa gagtgcatac caaatggatt catttactct
    54601 tcgtcagtta cgaagataat caaaatctcc aacttcccta cttatttcgt tttgagaatt
    54661 atcgaagcaa tcttacataa ataactaaaa aacaaaaaac cagaaaatca gtatactgat
    54721 tttctggttt tacatggttt gttcaataaa aagctgttgg aactgttaat attaaattat
    54781 cgcatcgtta cgaattcttc cgctcctgtt ggatggatgg ctactgtatt gtcaaagtct
    54841 tctttagttg cacccatctt gatagccacg gcaaaacctt gcagcatctc atcgacacca
    54901 acaccaatag catgtaagcc gacgattctt tcttcttttc cgacacagat caatttcatt
    54961 tcgcattttt ggcgataatc atttaatgca aagtacattg gggtaaagcg tgaacgataa
    55021 actttcaatt cattttcgcc atatttttcc aatgcttctt cttctgtcaa accaattgtt
    55081 gcaattggtg gatgcgtaaa gacgactgtc ggaacaagtt tatagtcaag ataagaatct
    55141 ttctttccat taaataaacg ttctgataaa cgtctaccag ctgcgattgc aacaggagtc
    55201 agatctaatt taccgatcac atcaccaaca gcaaaaatat gttcatccgt tgtattttgg
    55261 tatttatcta ctttgataaa cccttttttg tccaattcca catttgtatt ttccagacct
    55321 aatttttcgg tattcggtac acgtccaccg gcaaaaagta cacaatcacc agttaaggtt
    55381 tcaccatttt caaatagcac cgtatattcg tcattgtttt tagtaatttc tttagcaata
    55441 tgatgtgcgt aagtttttat tccagcttct tggtacattt ggatcaaatt gtccgaaagc
    55501 attttatcaa acgttctaag tggtcgttct tttcgatatg cccacatgac ttcactacct
    55561 aaaccacgaa atacacctga aagttcagcc gcaatgtatc cagcacctaa aacgattgct
    55621 ctcttaggta actctgttaa ttcaaaaaat ccatttgaat caattgcgta ttctccacca
    55681 ggaatggtca ttttacttgc acggcctcca gtagcgatca agattttagg agcacgatag
    55741 gtttcaccgt ttacttcgat cgtttgacta tcgataaaag tagcgtaacc ttttatagcc
    55801 tcgacatgat tgctatctaa tcctctttga taagcaccgt gtagaaaatc aatatatttt
    55861 tctcgtcttt ctactagacc ggcgaaatca acatttttta aatctgcttg gataccgtaa
    55921 cttgaggcat ctcgttcaat tgtttccaaa atcgtacttg cttgccacat aactttttta
    55981 ggtacacaac cgacgttgac acatgttccg cccaattcat tcgcttcaat cagcaaaact
    56041 tttgcgccat gcatccctgc tctgtttgct gaagcaatac cgccagagcc gccaccaatt
    56101 acgatgtaat catatatttg cattttgttc ctccttattg aagagatcaa tcaaaacctt
    56161 tatttaacga tcctttttct acagaaaaca agtgtaacac ttttcttgaa ttttgtatgc
    56221 gaagatgctt aaaacaggga tatatcagct ttttccttat ttacgttttc ggatcatatc
    56281 tccaggtttc accggctgtg cgactggcat agttaaaatc atcattggat ttggtgctcg
    56341 gtcaatggat tcgccttctt cattatgcat aacttcaacg gtttgactga aatgactgaa
    56401 tcctggacca taaaattcga tttcatctcc tacagaaaac aagtttctct gacgtatcgt
    56461 agcaatctgt gttgcctcat cataagataa tacttcaccg acaaatttat atactgggat
    56521 tttacgacgt tcaccgaata attgttcgtt ttcagttggt gtattgtagt aaaaacctgt
    56581 agctaattct cgttgtgcta ctttccataa ctcgtcgatc cattcttgct tgcagacata
    56641 gttttcagga tcttccatat aactgtctac agccgcttta tagacatttg acaccgtaga
    56701 tacataatgg atcgatttca ttcttccttc gatcttgaag ctgtctacgc cgttttcaat
    56761 caattctgga atatgttcga tcattgacat atccactgca ctcatcgaga attcttcttc
    56821 cacttcgcct gtatcagtta aactgtttcg ttcttgacca aaaggcatat cgaacaatcc
    56881 atacttccag cgacaagatt gagaacatcc gccacggttt gcatcccgca tcgacatatg
    56941 gttggacaat gtgcatcgac cagaataaga gatacacatg gcaccatgga taaatgcttc
    57001 gatttcggta tccgtatttt tacgaatctc tgccacttcc tccattgaaa cttcacgagc
    57061 taaaacgaca cgttccaacc cttcttcttt ccagaattcc aatgtctcaa aattagttgc
    57121 tgaagcttga gtggataaat ggatcggcag acctggggct tctgtcgcac aaatttcgat
    57181 caaagctgga tcagaaacaa tcactgcaga aataccgaca tcacgcaaac tgcggaaaaa
    57241 ttcccctgct ccatcttcat ttccttcatg ggtgaccata ttagcagcaa cgtatacctt
    57301 cgcatcatgt tcttttgcaa aagcaactcc ttcagccatt tcttcataag taaaattacc
    57361 tgcacggctt cttaaaccat atgcattccc gccaatatat actgcatctg ctccataatg
    57421 gatcgcggtt tttaactttt ccagtgttcc tgctggcgct aggatttctg gtcgtttcaa
    57481 ttttcttttt actgtcattt ctttcatcat actccttatt tgatagcaga aggatcaata
    57541 taaaagaatc ctgtatctaa ttcacggttt tcaggatgca atttactgat ttcgctagtc
    57601 attttttccg ctttttcttg atcccagcta ccttctaaaa tttgtttttt tgcttcggcg
    57661 aatagtcgag tgatgtttac gaatttttcg cctggagtaa ataatccgtc caatttccat
    57721 gtacagtaat gatgctgata aagttcattc aattcattcg agagattcag atcattgctg
    57781 gcaaaaatat gtgtgccatg actatcttca taaatagaat aatgtgtctc ttctttttta
    57841 ggttccgaga taaaaagacc tctttcacga tctttttgtt cgtcttgttt cgtatagtta
    57901 tagtaattct gtagcagtgg acgtttggat tgatggatgc atgtagcacc atacacaagg
    57961 gtttctacag gaatatcgag tttttcttcc atcgccttca tctcttcaaa aggaacttca
    58021 cgagcaagta ctgctccaac tgcgcctttt ttcgcccaga aattgatttg acgtgcgctc
    58081 gttactaatg tttcagcatc ataaatatac ggtatacgca attcaggatt ttttttcatc
    58141 gtataaataa tacctgtatc accaactgtg atcacatcta cgtttatttc ttttaaaaaa
    58201 gccaaatatt caggtacatt tttcattttt tcaggatgaa tgatcccatt tacagcaata
    58261 ttcgcttttt taccatactt gtgtgctaat tctaccaatt gtcgctgttc ttctcgtgta
    58321 aatgaagcag gcagacgcaa tccaaattgc tcttcaccga aatatagtgt atctgcacct
    58381 gcagcaagca actgttctgc ttgttcaata gtttctactg tggttatgag ttcgatcata
    58441 attttctcct cctacaactt cataatagta gcatagctct aaaaactttc caatacttct
    58501 tctatttaaa gtcggatttt tgaaaattat ttcacaaaaa atcaaaataa agttcgatat
    58561 attctgtcca aatataaaaa gagattgtga caaaaatctt gtcacaacct cttaatcaag
    58621 tcttactatt ataaaaattc agctacacag gccaagtaac tatagggaac ctactgtttc
    58681 ctttttaggt ccgcgaacat tcaatgtgat cttacctttt gaagcaccta ctgttacgtg
    58741 gtcacctaaa cgaatttctc cgccaagcaa agcctcgctc aatcgatctt caatttcttt
    58801 ttgaagtgcg cgacggattg gacgtgcacc atattcagga tcaaatccag ctttcccgat
    58861 cacatcgatt gcagatggtg tgattttcac ttgaatatct tgttctttca atcgtttgat
    58921 aatcgctttg ctcatgattt ggacgattgt atggatttca tctttactca atgaatggaa
    58981 gacgacagtc tcatcgattc gattcaagaa ttcaggtcgg aaagctttct ttaactcttc
    59041 taagatacgt ttttgcatgg ctttgtgatc tttagtaaca tcttggacat tgaatccgac
    59101 atttttttct tctcgaattt gtgtcgctcc gatatttgaa gtcataatca tgattgtatt
    59161 tctaaagtcc acttttctac ctttagaatc tgttaaatga ccatcatcca atacttgcag
    59221 aagtaaattg aaaacgtctg gatgtgcttt ctccacttca tctaagagaa tcactgaata
    59281 tggtttttga cgaatttttt ctgtcaactg tccaccttca tcatacccta cgtatcctgg
    59341 aggagacccg attaatcggc ttgtactgta tttttccata aattctgaca tatcaacacg
    59401 gatcagggca tcttcacttc caaacatgac ttctgaaagt gctttggcca attctgtttt
    59461 tccgacacca gttggtccaa ggaacatgaa tgaaccaatt ggacgatcag gatctttcaa
    59521 tccgcttcgt gcacggcgga tcgcacgtga aactgctttg acagcctcat cttgaccaac
    59581 gacacgttca tgcagaagtc cttccagttc taacaagcgt tcgctttctt ttttctccaa
    59641 ctgctgtaga gggacaccag tccactccga tacaactgta gcaacatctt cttctgttac
    59701 acgatcagaa taaccttgct cctgttttgc ttcaattgca gaaacttttt gcagcttttt
    59761 acgtagtgct ttttcttgtc tgcgtaattg agcagcattt tcaaagtctt gtttttggat
    59821 cgctgcttcc ttttcttgta ctagttcatc gatttctagt ttgatcacag ttgattttgt
    59881 taagtgatcg gtttgatcca aacggacttt cgctgctgat tcatcgatca aatcaattgc
    59941 tttatcgggt agttgacgag aagtgatata acgaacagaa agattgactg ctgctcgtac
    60001 agcttcatct gtgatctcca ctccatggtg ctcttcatat cttgtacgta atccttgaag
    60061 aatcacttct gcttcttcag gagttggctc atctacttga atgcgagcaa aacgtcgctc
    60121 taatgcagaa tctttttcga tatatttttg atattcatct agggtcgttg ctccgatcgt
    60181 ttgtaattct cctcgagcta acgccggttt caagatattc gaagcatcaa ttgctccttc
    60241 tgctccacct gcaccaatta aggtatgcaa ttcatcaatg aataagatta cttgaccatc
    60301 attatagatt tcgtcgatga cctttttcat tcgatcttca aattcaccgc gatatttcgt
    60361 accagcaacc aaagatccca tatcaagcat catcaggcgc ttttcttgca tgtcttctgg
    60421 cacttcacct aaaatgattt tttgagctaa cccttcagca atggctgttt tgcctacacc
    60481 aggttcaccc accagtacag ggttattctt tgtccgtcgg ctaagaatct ggataagccg
    60541 tttcacttct ctcgaacgac caacaactgg gtccagcttg ttttcttttg ctaatttcgt
    60601 caaatcacgt gctaatgaat ctagcgtagg tgttccttgc ggtgtttgcc gtttatcttg
    60661 tacatttaca tttctacgac gattcattcc ttttccagca gaatcaccag ctagccccat
    60721 tttttgtttc agaacttgac gggcttttga aaggctgata cctaagttga tcatgattcg
    60781 agtagccatg atactttcat cttttaataa gctcatcaaa atatgttctg ttccaacttt
    60841 ttgagaacca gttttcttca cttcgctgct agctaaagca aaaacttgtt tcattcttgg
    60901 tgaatatgga agaaagactc ctttagggta agaacggata ccaccataac caatcagatg
    60961 ttcaatttct tcataaattt ccgtttcatt caaatccatt tcacgtaata cttttccagc
    61021 aattccattt tgctcgacca ctaaggctag taaaacatgt tctgaactaa ctgattgatg
    61081 tttcaaccgt tttgcttctt cttgtgcaat ttctaatact gcttgggctc tttcagtaaa
    61141 aagttcttcc atctaatctc acttcccatc ttcataactt aaacgttcca gaaatgctcg
    61201 gagaatccga gcacgtgtat gatcttcata ttcattgaaa ttcaatgtgt tttttgcaat
    61261 tgcactcaac atcaagtttc cttctttttt tgtaattatt tggtcttcat aaagtttttg
    61321 aataatagca aaagcatttt tctcactgat tgtatcatca aataactgat ttatctgctc
    61381 aagtaattgt tttttatctg aaatccttac ttttgcaatt cgtatataac caccaccacc
    61441 acgtttgctt tctactagat aaccacgttg gatggtaaaa cgggtattaa tcacatagtt
    61501 gatttgtgaa ggaacacagt taaagaggtc tgccatttca atccttcgaa tctcgatcat
    61561 ttcactattt tccagaattt tctttatata tgattcaatt aaatccgagg tattctgatg
    61621 tgccatctta ttcattccct ttgactaata ttgaccttaa ttataacgaa tttgataata
    61681 gaaagaaaga agaacgcttt ttgttaaata aatcctttag aatcaacaaa aaaaaatacg
    61741 gaatatacta ttccgtatc
//
LOCUS       DACCXO010000005        58293 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.005, whole genome shotgun sequence.
ACCESSION   DACCXO010000005 DACCXO010000000
VERSION     DACCXO010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.005"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            131..502
                     /locus_tag="IVY70_000250"
     CDS             131..502
                     /locus_tag="IVY70_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP7957413.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(776..1468)
                     /locus_tag="IVY70_000251"
     CDS             complement(776..1468)
                     /locus_tag="IVY70_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7957414.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1471..2256)
                     /locus_tag="IVY70_000252"
     CDS             complement(1471..2256)
                     /locus_tag="IVY70_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7957415.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2493..3893
                     /gene="lacG"
                     /locus_tag="IVY70_000253"
     CDS             2493..3893
                     /gene="lacG"
                     /locus_tag="IVY70_000253"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP7957416.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            3918..4235
                     /locus_tag="IVY70_000254"
     CDS             3918..4235
                     /locus_tag="IVY70_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7957417.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4248..5921
                     /locus_tag="IVY70_000255"
     CDS             4248..5921
                     /locus_tag="IVY70_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7957418.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            5935..7566
                     /locus_tag="IVY70_000256"
     CDS             5935..7566
                     /locus_tag="IVY70_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP7957419.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8057..9622)
                     /gene="guaA"
                     /locus_tag="IVY70_000257"
     CDS             complement(8057..9622)
                     /gene="guaA"
                     /locus_tag="IVY70_000257"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP7957420.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9681..10604)
                     /locus_tag="IVY70_000258"
     CDS             complement(9681..10604)
                     /locus_tag="IVY70_000258"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP7957421.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10800..11405
                     /locus_tag="IVY70_000259"
     CDS             10800..11405
                     /locus_tag="IVY70_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7957422.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11713..12729
                     /locus_tag="IVY70_000260"
     CDS             11713..12729
                     /locus_tag="IVY70_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7957423.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            12870..14171
                     /locus_tag="IVY70_000261"
     CDS             12870..14171
                     /locus_tag="IVY70_000261"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP7957424.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14217..14879
                     /gene="deoC"
                     /locus_tag="IVY70_000262"
     CDS             14217..14879
                     /gene="deoC"
                     /locus_tag="IVY70_000262"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP7957425.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            14900..15298
                     /locus_tag="IVY70_000263"
     CDS             14900..15298
                     /locus_tag="IVY70_000263"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP7957426.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15364..16446
                     /locus_tag="IVY70_000264"
     CDS             15364..16446
                     /locus_tag="IVY70_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7957427.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16658..18220
                     /locus_tag="IVY70_000265"
     CDS             16658..18220
                     /locus_tag="IVY70_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7957428.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18213..19346
                     /locus_tag="IVY70_000266"
     CDS             18213..19346
                     /locus_tag="IVY70_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7957429.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19346..20302
                     /locus_tag="IVY70_000267"
     CDS             19346..20302
                     /locus_tag="IVY70_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7957430.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20401..21567
                     /gene="deoB"
                     /locus_tag="IVY70_000268"
     CDS             20401..21567
                     /gene="deoB"
                     /locus_tag="IVY70_000268"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP7957431.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21591..22409
                     /locus_tag="IVY70_000269"
     CDS             21591..22409
                     /locus_tag="IVY70_000269"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP7957432.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22426..23133
                     /gene="deoD"
                     /locus_tag="IVY70_000270"
     CDS             22426..23133
                     /gene="deoD"
                     /locus_tag="IVY70_000270"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP7957433.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23431..24117)
                     /gene="gpmA"
                     /locus_tag="IVY70_000271"
     CDS             complement(23431..24117)
                     /gene="gpmA"
                     /locus_tag="IVY70_000271"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP7957434.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24289..24870)
                     /locus_tag="IVY70_000272"
     CDS             complement(24289..24870)
                     /locus_tag="IVY70_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7957435.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25003..25686)
                     /gene="rpiA"
                     /locus_tag="IVY70_000273"
     CDS             complement(25003..25686)
                     /gene="rpiA"
                     /locus_tag="IVY70_000273"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP7957436.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26005..26418
                     /gene="rpsL"
                     /locus_tag="IVY70_000274"
     CDS             26005..26418
                     /gene="rpsL"
                     /locus_tag="IVY70_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP7957437.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26502..26972
                     /gene="rpsG"
                     /locus_tag="IVY70_000275"
     CDS             26502..26972
                     /gene="rpsG"
                     /locus_tag="IVY70_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP7957438.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27066..29153
                     /gene="fusA"
                     /locus_tag="IVY70_000276"
     CDS             27066..29153
                     /gene="fusA"
                     /locus_tag="IVY70_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP7957439.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29325..30512
                     /gene="tuf"
                     /locus_tag="IVY70_000277"
     CDS             29325..30512
                     /gene="tuf"
                     /locus_tag="IVY70_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP7957440.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            30919..31227
                     /gene="rpsJ"
                     /locus_tag="IVY70_000278"
     CDS             30919..31227
                     /gene="rpsJ"
                     /locus_tag="IVY70_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP7957441.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31258..31887
                     /gene="rplC"
                     /locus_tag="IVY70_000279"
     CDS             31258..31887
                     /gene="rplC"
                     /locus_tag="IVY70_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP7957442.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            31915..32538
                     /gene="rplD"
                     /locus_tag="IVY70_000280"
     CDS             31915..32538
                     /gene="rplD"
                     /locus_tag="IVY70_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP7957443.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32538..32828
                     /gene="rplW"
                     /locus_tag="IVY70_000281"
     CDS             32538..32828
                     /gene="rplW"
                     /locus_tag="IVY70_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP7957444.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            32867..33700
                     /gene="rplB"
                     /locus_tag="IVY70_000282"
     CDS             32867..33700
                     /gene="rplB"
                     /locus_tag="IVY70_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP7957445.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33743..34021
                     /gene="rpsS"
                     /locus_tag="IVY70_000283"
     CDS             33743..34021
                     /gene="rpsS"
                     /locus_tag="IVY70_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP7957446.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34044..34391
                     /gene="rplV"
                     /locus_tag="IVY70_000284"
     CDS             34044..34391
                     /gene="rplV"
                     /locus_tag="IVY70_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP7957447.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34405..35061
                     /gene="rpsC"
                     /locus_tag="IVY70_000285"
     CDS             34405..35061
                     /gene="rpsC"
                     /locus_tag="IVY70_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP7957448.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35064..35498
                     /gene="rplP"
                     /locus_tag="IVY70_000286"
     CDS             35064..35498
                     /gene="rplP"
                     /locus_tag="IVY70_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP7957449.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35488..35676
                     /gene="rpmC"
                     /locus_tag="IVY70_000287"
     CDS             35488..35676
                     /gene="rpmC"
                     /locus_tag="IVY70_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP7957450.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35701..35967
                     /gene="rpsQ"
                     /locus_tag="IVY70_000288"
     CDS             35701..35967
                     /gene="rpsQ"
                     /locus_tag="IVY70_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP7957451.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36025..36393
                     /gene="rplN"
                     /locus_tag="IVY70_000289"
     CDS             36025..36393
                     /gene="rplN"
                     /locus_tag="IVY70_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP7957452.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36430..36738
                     /gene="rplX"
                     /locus_tag="IVY70_000290"
     CDS             36430..36738
                     /gene="rplX"
                     /locus_tag="IVY70_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP7957453.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36766..37305
                     /gene="rplE"
                     /locus_tag="IVY70_000291"
     CDS             36766..37305
                     /gene="rplE"
                     /locus_tag="IVY70_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP7957454.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37324..37509
                     /locus_tag="IVY70_000292"
     CDS             37324..37509
                     /locus_tag="IVY70_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP7957455.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37545..37943
                     /gene="rpsH"
                     /locus_tag="IVY70_000293"
     CDS             37545..37943
                     /gene="rpsH"
                     /locus_tag="IVY70_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP7957456.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            37975..38511
                     /gene="rplF"
                     /locus_tag="IVY70_000294"
     CDS             37975..38511
                     /gene="rplF"
                     /locus_tag="IVY70_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP7957457.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38672..39028
                     /gene="rplR"
                     /locus_tag="IVY70_000295"
     CDS             38672..39028
                     /gene="rplR"
                     /locus_tag="IVY70_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP7957458.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39049..39549
                     /gene="rpsE"
                     /locus_tag="IVY70_000296"
     CDS             39049..39549
                     /gene="rpsE"
                     /locus_tag="IVY70_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP7957459.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39564..39743
                     /gene="rpmD"
                     /locus_tag="IVY70_000297"
     CDS             39564..39743
                     /gene="rpmD"
                     /locus_tag="IVY70_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP7957460.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39787..40227
                     /gene="rplO"
                     /locus_tag="IVY70_000298"
     CDS             39787..40227
                     /gene="rplO"
                     /locus_tag="IVY70_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP7957461.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40227..41522
                     /gene="secY"
                     /locus_tag="IVY70_000299"
     CDS             40227..41522
                     /gene="secY"
                     /locus_tag="IVY70_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP7957462.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41584..42231
                     /locus_tag="IVY70_000300"
     CDS             41584..42231
                     /locus_tag="IVY70_000300"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP7957463.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42422..42640
                     /gene="infA"
                     /locus_tag="IVY70_000301"
     CDS             42422..42640
                     /gene="infA"
                     /locus_tag="IVY70_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP7957464.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42673..42789
                     /gene="rpmJ"
                     /locus_tag="IVY70_000302"
     CDS             42673..42789
                     /gene="rpmJ"
                     /locus_tag="IVY70_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP7957465.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42807..43172
                     /gene="rpsM"
                     /locus_tag="IVY70_000303"
     CDS             42807..43172
                     /gene="rpsM"
                     /locus_tag="IVY70_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP7957466.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43200..43589
                     /gene="rpsK"
                     /locus_tag="IVY70_000304"
     CDS             43200..43589
                     /gene="rpsK"
                     /locus_tag="IVY70_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP7957467.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43669..44607
                     /locus_tag="IVY70_000305"
     CDS             43669..44607
                     /locus_tag="IVY70_000305"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP7957468.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44635..45015
                     /gene="rplQ"
                     /locus_tag="IVY70_000306"
     CDS             44635..45015
                     /gene="rplQ"
                     /locus_tag="IVY70_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP7957469.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45287..46126
                     /locus_tag="IVY70_000307"
     CDS             45287..46126
                     /locus_tag="IVY70_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7957470.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46102..46971
                     /locus_tag="IVY70_000308"
     CDS             46102..46971
                     /locus_tag="IVY70_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7957471.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            46961..47758
                     /locus_tag="IVY70_000309"
     CDS             46961..47758
                     /locus_tag="IVY70_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP7957472.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            47961..48707
                     /gene="truA"
                     /locus_tag="IVY70_000310"
     CDS             47961..48707
                     /gene="truA"
                     /locus_tag="IVY70_000310"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP7957473.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(49162..51831)
                     /locus_tag="IVY70_000311"
     CDS             complement(49162..51831)
                     /locus_tag="IVY70_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP7957474.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(52069..52257)
                     /locus_tag="IVY70_000312"
     CDS             complement(52069..52257)
                     /locus_tag="IVY70_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP7957475.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            52527..52889
                     /locus_tag="IVY70_000313"
     CDS             52527..52889
                     /locus_tag="IVY70_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7957476.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            52941..54623
                     /locus_tag="IVY70_000314"
     CDS             52941..54623
                     /locus_tag="IVY70_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP7957477.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            54740..56776
                     /gene="recG"
                     /locus_tag="IVY70_000315"
     CDS             54740..56776
                     /gene="recG"
                     /locus_tag="IVY70_000315"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP7957478.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            56825..57826
                     /gene="plsX"
                     /locus_tag="IVY70_000316"
     CDS             56825..57826
                     /gene="plsX"
                     /locus_tag="IVY70_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP7957479.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            57948..58190
                     /gene="acpP"
                     /locus_tag="IVY70_000317"
     CDS             57948..58190
                     /gene="acpP"
                     /locus_tag="IVY70_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP7957480.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT        18591 a        10631 c        12746 g        16325 t
ORIGIN      
        1 gagaacgggt tggtttttaa tagatttaat aagtactgat aatgaaattt ctcctggttg
       61 ggaaaacgaa gttggttacc ctgtagaaga attttttcaa gattactttg cggagtatgc
      121 cgataggata atgagagatt acgtaacaga taaaactcaa gaagtcatca atcaatccca
      181 gtcattaaat gaattatctg ataaggcgaa agaagaagca gttgccatta tttcttcagg
      241 gattagtgac tatattgata tgagtggtga aatcaattat ggcgataaag acaaaattgt
      301 acgcatgata aaagctttta tcaaaacttt tgatgtgaag gcagatctta agtttaatga
      361 tacttaccta gtaggaaaat taattaatat ccttgggaca gagaaagata cggaaacgtt
      421 tatttcctct ttgtctatgt tgtttacaga taagcatttt tcaactcagc ttggtggaga
      481 ggaactagtg gaaatctttt aaccattacg cccagcatta ataaaagtat atcaaaaaag
      541 tactcctgat gatttttaag attggtttga gaaataatgt gaaaacacta tttttaatgt
      601 taattataga tagagatgaa cgcgtcatat ggttaaaaga atgaaaaagt caaagataca
      661 ctgctatatt tcactaagga tatagcagtg tatctttcat gtcgtttcac tggcagtttt
      721 ttataaaagg aggtgtgcat ttcagttgct tttctttggt agttagttga atcgtttaag
      781 aattaacgga tgtggcagac aggtgctctg tccaaaaacc agtaaaatct ttgatggttc
      841 catctttatt tagctgtgcc tgttcaaata ctgttttgtt tttaccgaat ggatcaattg
      901 caataattgt tccgatcccc gcacgtttgg cagcaagtaa tcctgagtaa gcatcttcga
      961 taacaagaca atcagccgga gctaagctca gtttgtctgc cgctttaaga aaaatatccg
     1021 gttgcggttt cccagggaaa gagccgtcgt catagacaat cttgtcccag tcaaaccagc
     1081 gggctaaatc gaaaatatca aaataaaaag caacattctc tttgacagta gccgtggcaa
     1141 tcgttaatgg aatctgttgg tcagtcagct ggtcaagagt cgcttcaagc ccttttgtca
     1201 actgaagctc gttttcattt tgcaagcaaa gcatacgata atagctttct ttttcttctg
     1261 acaatacggc gatctcagag gcggtcaaat gctctgaaat gaaatgtttc aaaatctccg
     1321 tattcgtccg accatgaata ttttttaaaa tatcctcgtc tgataaacca tcttttgtat
     1381 gatcatgtat catatgcagc caagcagctt catgcagatg agagtccaaa aacattgttc
     1441 cgttgaaatc aaatataatc cctttcatag ttacgctcct atagtggtat tttcggttga
     1501 atcagcaatt tgcagatcga catattgtgc atagtgctct tgaacctccc gcgagatctg
     1561 gcgatccgta atgagcagat cgaaatcatg caaattataa aacacataaa aatcatctcg
     1621 attgaatttt ttattatcaa cgagtagata tttcttacgg gaattattta gagcgatctg
     1681 ctgtgcttct ccttcttcgg tactgtaagt agagatcgct tcgttgtgaa cgccatttga
     1741 actgataaat gcttttgtaa actttagtct tcttaaattg tcgttggtga tcggtccgac
     1801 aaatgcgccg gtatttttcc ggaaatcacc gccaatcagt agaatttctg gtccatctgc
     1861 ttgagcaaga atatcaaaaa ctggataatt atttgtaatg atccgaattt tcttgttcag
     1921 taaatgcgtc gccaatagtt cgattgttgt acctggtcct aagaaaatcg tttctccctc
     1981 gtgaatcaag gaggctgctg tggcagcaat ttcttttttt tcttgaatct gcagagtggc
     2041 tttttcagtg tgagaaagtt cttgatctaa agtgtaggaa agactttgag cccctccatg
     2101 gacacgcagc agcttgcccg atttttctaa ttcatctaaa tctctgcgaa cggtcatatc
     2161 agaaacatct aattgattga tgatttcatt aacggtaagg attccttttt tattgaccat
     2221 ttctacgata gtaagcaatc tttctttttt caacattgtc ttcacctcta atttaaggat
     2281 aatctatcat ataaacggac aacagtcaat ctaattcgaa caaaagtaaa catttagggt
     2341 ggtttttatg tttgttacaa tagattttat tgctgttttc tggtttttat gattatttgt
     2401 gtttgttttt gttgacaaat gttttggatg cgtttacaat gcaatcagtt agaaagagcg
     2461 aaaactgatt atagatttga ggagagatgt acatgcttaa attaccagaa gattttattt
     2521 ttggcggagc cacagcagcc tatcaggttg aaggagccac taaggaaggt ggcaaaggcg
     2581 ctgttgcttg ggatgacttt ttagaggaac aagggcgatt cagtcccgat ccggcaagtg
     2641 atttttatca tcaatatgca aaggacattg aattgtgtga acgttttggt gtaaatggtc
     2701 taagattatc cattgcttgg agccgcattt ttcctgatgg agctgggaaa ccaaatccag
     2761 aaggaatcgc tttttatcat cgtgtgttcg aagaatgtaa gaaaagaaac gtaacgccgt
     2821 ttgtgacgtt gcatcatttt gataccccta aacgtttgtt tgaccatgga gattttttga
     2881 atcgagaaac aatagaagca tttgtttcct atgccatttt ttgttttcat gaattcaaag
     2941 aagtcaaggt gtggagtacc tttaatgaaa tctatccagt tgcaaccaat caatatttgt
     3001 taggtgtttt cccaccagga atcaaatatg attttacaaa gatcgtcgcc tgtcttcata
     3061 atatgatggt tgctcatgcc cgtgtagtga actatttcaa agaaaacgaa cttccgggtg
     3121 aaattggtgt agttcactca ttagagacga aatatgcggc aacagacgcg ccggaagata
     3181 agcatgctgc ctttttagac gatgcattgt cgatccgttt cttattagat gctacctatt
     3241 taggctatta ttcaactgaa accttgactg ctcttgatga gatatgtgaa gcaaatcagg
     3301 ccagctatca ttttcctgaa gaagatttcg ttgaattgaa aaaagcatcc acgcgtaatg
     3361 actacttagg gatcaatcac taccaatgcc attttgtcaa agcttacgat ggagaaaatg
     3421 caatccatca taacggtacg ggtgaaaaag ggacctctgt ttataaagtg aagggaatcg
     3481 gagaacgtat ttataaagaa ggaattcctc gaactgattg ggactggctg atttatccag
     3541 aaggtctata tgacttgttg ctgcgaatta aaagcgatta cccacattac aacaaaatct
     3601 atatcactga gaacgggatg gggtataaag atcagtttga agatggcatt attatggatc
     3661 agccgagaat cgactatttg agagtctatt tggaatcctt aagcaaggcg ataacagccg
     3721 gggtaaatgt gaaggggtat tttttgtggt cgcttatgga tcttttctca tggacaaatg
     3781 ggtataacaa aaggtatggc ttgttttatg ttgattttga aacccagaag cgttacccaa
     3841 aagaatcagc ttactggtac aagttggtca gtgaaaccaa aacgattatt taataagtga
     3901 taatggagga agaaaagatg aataaagagg aattacaaat gcttggtttt gagatcgttg
     3961 catattcagg cgacgctcgc agtacattat tgaaattatt gaaagaagta agacaaggaa
     4021 actttgaaca tgttgattcg gcgttgaaag aagccgacga aaatttaacc cttgcacata
     4081 attcgcaaac aaaaatttta gcggaagaag catctgggaa agagatggag atgggattca
     4141 tttttatcca tgggcaggat catttaatga ccactttatt gctgagagat ctgattcaag
     4201 actttattgt actgtacaga cagaatcaag gataggagaa gaacgctatg gatggaatca
     4261 ttaaacaaat cgaaaaaggg aaaccatttt ttgaaaaaat ctcacgtaat agttatttag
     4321 gagccgttcg tgacggattt ttaacagcga tgccagctat tttattttca agcattttca
     4381 ttttgattgc tgcggttcct gagatttttg gttaccaatg gcctgaatct gtctccacat
     4441 ggctatggaa agtctataat tattcgatgg gtgttgtagg gttactcgtt tcagcaacca
     4501 ctgctcgctg cttggctgat tcgatgaatc gtcgcttacc gaaaaacaaa gtaatcaaca
     4561 ccacctctgt gatgctggcc tctgtggtaa gtttcctgtt gttaagcgtc tctccgattg
     4621 aaggtggatt ttcaacggaa tatatgggga caaagggact actggcttcc tttgtcgctg
     4681 catttttaac agttaattta tataagtttt gtgtgctgaa agatattacc attaaaatgc
     4741 ctaaagaagt tcctggaacg atttcgcaaa ccttccgaga tatttttcct ttctcttttt
     4801 cagtatttgc ggccgtttta attgatttag gcattcgaca aatcttccaa atctcatttg
     4861 cagaagcaat cattacggtg ctgcagccgc tatttacagc agctgacggg tacttaggaa
     4921 tcgctattat ctggggagcg atggcgctgt tttggtttgt cggtgttcat ggaccgtcga
     4981 tcgtggagcc ggcaattgcg gcaattatct atgccaatgt ggaaaccaat ctgcaattgt
     5041 tcaattccgg tgaacaagct tctaaagtac tgacggtggg tttagggaat tttgtaggaa
     5101 ctatgggagg aactggggca acattggttg ttccttttat cttcctactc tttgctaagt
     5161 ctaagcagtt gaaggcagtt gggaaagctt cctttattcc agtaagtttc gctgtcaacg
     5221 aaccattgct atttgcggca cccatgattt tgaaccctta tttttttatt ccatttttgt
     5281 taacacctgt tgtaaacgtt tgcctgttca agttttttgt cgatgtattg ggcatgaaca
     5341 gctttatgta cgttttgcct tgggcaactc cagcaccgat cggtttggtt ctaggaacgg
     5401 gcatgggtat cttagctttt gttctagcag cggttctaat cgtcgttgac ttcctgatct
     5461 atctaccatt ttgcaaagca tacgatgctg tcttagtaga gaaagaagca caaaaagcag
     5521 aggtgatcgc aaccgaggaa ggtgcttctg ccgatgtacg tgaggaagta cctgtggcag
     5581 cgagtcaagt tatcagcgat gcaaataaag aaatcaatgt gttggtactt tgtgctggcg
     5641 cagggacaag tgggatgtta gctaatgctt taaaaaaagg tgcatctgaa tcgaaagcac
     5701 cgatcagtgc aatggcgggt gcttatggtt ctcattacga aatgctgcag gattttgata
     5761 tggtggtctt agcaccgcaa gtcaattctt actatgatga tatcaaagaa gacaccgatc
     5821 gcttaggaat caagctggtt gcgacaaaag gagcagaata tatcggatta acgagagatc
     5881 ctgaaaaagc tgttcagttt attctgaacc aatttcaata aaggaggagc cataatgaaa
     5941 atgggaattt ggttgcgtaa agctgtttta tttgcagctg tgttaggggc tttgggggct
     6001 gtttcaaaaa ctgttgaagc atctgccagc gaatttattc gcggagcaga tatttcgatc
     6061 ctgtcagata tggaaaaatc tggtgcaaaa tattatgaag acgatatttc aaaagatgcc
     6121 ttagagattt taaaaaataa tggtgtcaac tatgttcgac tgcgtttgtg gcaggatcct
     6181 tataatagta atggagaaag ttatggagca gggacaaatg atctggaaac gacgattgac
     6241 cttgcaaaac gagcaaaaaa tctgggactt aaggtgctgc tggattttca ctacagtgat
     6301 ttttgggttg atcctgggaa acagaatctt cctaaagcat ggcaaggtct gacctttgaa
     6361 gaaatgaata cggcattgta cgactataca aagaacgttc tgtcggaaat gaagcagctg
     6421 gacgtttatc ctgatatggt tcagatcggc aatgaactga atagcggaat gctctggcct
     6481 tatggcaagt cttggggaga aggcggggga gaatttgatc gattggctgc ttttttgaag
     6541 tctggtatcc aagctgttcg agacactcaa cctaaaacaa ctccagtaat gctccactta
     6601 gctgatggtg gcgatacagg agcatttact tggtggtttg atgaaatcac gagtcgaggt
     6661 gtgtcatttg atttaattgg agtgtcgtat tatccgtatt ggcacggttc aatgaatgaa
     6721 cttcaaacca atatgaatgc gatcagccaa cggtatggta aagaagtgat cgtagttgaa
     6781 acagcttatg ggcatacaac agcgaatgcc gatactatgc caaatgcttt tggtgaagcc
     6841 gaagcagctg cggggggata tgaaccaaca ccgacaggac aagcagagta tttgctggat
     6901 cttgccgatc ggattcaggc agtaccgaat aatcgcgggg cgggcttctt ctattgggaa
     6961 cctctttggt acaacggaaa tgtgagttgg gcaacacaag caggtatgaa ttatctcagt
     7021 gttcaatcca ctatgggaaa cgaatgggac aaccaagcga tgtttgattt tgctggaaac
     7081 gccttgccaa gcttgcgtgc gtttaaacaa gcaggggcgc aaataaatct agttaaaaat
     7141 gcaagtttcg aaagcgatgg accaacgacc agtccttcca attggcaaac ctggtttcaa
     7201 caaggaaccg ctaagggtac gatcaaaacg gaagcgggca ctttttttgg ggactacaaa
     7261 ctaacttttt ggcaggatca agcatacgag gcatcagctt atcaaaatgt tagtggcata
     7321 agcaaaggaa cttacacatt gagtgcctat gtaatgtctg ggggagagtt tgatactaat
     7381 cagttatata tcaaaaatta tggtggagcg gaactaaata ctgcggttgt taaaacaagt
     7441 gaatggacaa aaattacaat caaagacatt ccaattacaa caggtgtttg tgaaattggg
     7501 gtttatagca aggcttcagg aaatgcttgg acgaacgttg attttgtttc attggtgaaa
     7561 caatagactg cggatcgttt cgattcaaga tcggaggttc ccttcagcca catcgttagc
     7621 gtagaaggca ggaatcgaac ctgcacctcg cctttgccaa aaataaccag aaacgatttc
     7681 cagtcatttt taccaaaacg agtaaagcac cagacttcat ctacactttg tattatgcta
     7741 aagagagttc aacgatttcg taagaagcaa tgaaaaaatt gcaaaaagtt gtgcagataa
     7801 cataaagttt gtttaagaaa gggtaacctt agaattccgt cgtgaatatg atttgagcac
     7861 ttagatggat agaaaaaagt tctcggaaaa cagttcgttt ttcgagaact tctttttatg
     7921 ataagaatga aacaaattaa cttatattat agtctctttt ggtagggaac catcttattt
     7981 ttaatgaaaa tcaagcattt tagctattga agaatgttga tttataggga ttccccctcc
     8041 atattatgtt ggctctttat tcccactcca ctgttgctgg tggtttagaa gtgatgtcat
     8101 acacgatacg gttgacgtgg gctacttcgt ttacgatacg tacagagatt ttttgcaaga
     8161 cttcccatgg gatacgggca aagtcagcgg tcattccgtc gattgaagtt acagcacgga
     8221 taccgactgt gtagtcgtaa gtacgtccgt cacccataac accgacagag cggatccctg
     8281 gaagaacagt gaaatattgc cagatatcac ggtcaagtcc agcagctgcg atttcttcac
     8341 gcaagatagc atcagattcg cgtacgattt ctagcttgtc ttcagtgatt tcgccaagaa
     8401 cacgaatacc aagaccagga ccagggaatg gctgacgcca aacgattgaa tcaggcatgc
     8461 ctaactgagt ccctaattca cggacttcgt ctttgaataa tgtgtttaat ggttcgatca
     8521 attcaaattg catatcttct ggcagtccac ctacattatg gtgagatttg atcgtctgtg
     8581 ctgtttctgt accagattcg ataacgtcag tatacaacgt tccttgtgct aggaaagaga
     8641 cgccttcttc accagcaagt tttgttgctt cgtcatcaaa aacatatacg aattcattcc
     8701 caatgatttt acgtttttgt tctgggtcag atacaccttt caacttgtct aagaagcgat
     8761 ctcttgcatt gactttgatg atgttcaagc cgaattttcc gcctagactt tccattactt
     8821 gttccgcttc gcctttgcgt agcagaccgt ggtcaacaaa gatagatgtc aattggtcac
     8881 cgatcgcttt ttgtaaaaga accccaacga cactagaatc aacgccacct gaaagaccaa
     8941 gcaagacttt tttgtcacca acttgttcac ggattttttc tacttgcata tcgataaagt
     9001 tatccattgt ccagttacct tcacagtgac aaacatcaaa ggcaaagtgg cgcagcaatt
     9061 cgattccgta ttcggaatga cgtacttctg catggaattg gataccataa aaattacgtg
     9121 ttgtgtcttg gatagaagca atcggacaat ctttgcttgt tccgactgtt tcaaagcctt
     9181 caggaacttt agttaccaag tcaccatggc tcatccatac tgtttgttgt tttggtgttt
     9241 ctttaaacaa gacagcttga tcgcttgtta cttctaacaa ggctttccca tattcgcggt
     9301 ttttagcagg ctctacggaa ccgccaagtt tgtacgtgat caattgcata ccatagcaaa
     9361 tccctaatac aggaatacct aaattgaaga tctcaggatc gatcccaaat gcattctcat
     9421 catatacact gtttggtccg ccagaaagaa tgattccctt tggattcatc gcttttactt
     9481 cttctgcagt aatgcggtgg ctcaataatt cggaaaagac tccaagatca cgaatacgtc
     9541 ttgtgatcaa ttggttatac tgactaccgt agtctaatac gataatcttt tcaacgttcg
     9601 tcatatcggc aacgtttgtc aaatttatta cccctatccc tttaaatttt atctcaaaat
     9661 agtcaaacta ttttaaaatg ctaatatttt cgcatgaaaa tcgaatcgat gatatgattt
     9721 tcagttttat ggagaattat atcggctctt cctcgtgtag gcaagatata ttcctgtaaa
     9781 tttttcaaat tcacattttt ccatacatct tttgccattt taaaggcttc ttcacgatct
     9841 ccgatagcaa aacgataata ataattctct ggattttgga aagccgtatc cagtaacgca
     9901 ccaaaacgat ccaaatacca tttttcgatc agcacgggat cggcatcgac atagacagaa
     9961 aaatcaaaaa agtcgctgac gtagatttgc tgattcgcag gtaattgaag cgtattgatc
    10021 ccctcaacga tcaagatatc aggctgctgg atcaattcgt acttgtctgg aatgatatca
    10081 tacacatcat gtgagtaaac aggtactttt atctcttcct gaccagattt tacacggttc
    10141 aagaaatcga tcagcatctc catatcataa ctttcaggaa atcctttacg gtccatgatt
    10201 ccacgctctt ttaatatacg atttggataa agaaatccgt cagtcgtgat cagctgaacg
    10261 tttcttcgtt tgaaggtacg agacaaaatc gtctgcagca gtctggctgt tgtacttttt
    10321 ccaacggcta cacttccggc gatcccgata ataaaaggcg gtacttctac gtatttatga
    10381 agaaataaac ctttactcaa gaacaaagat tcaaattctt tcatataaag ataaatcaga
    10441 tgagcaagag gtacatagat atctcgaaca tcctgcatgg aaatgcggtc attcaaactt
    10501 ttgatattat ctagttcatc ttgtgtcaaa ggtgcttgcc cattacgata aaattctcgc
    10561 cattcttctc ttgccacacg gtagtagttc attggatcat tcatattagc ctcctgcgtt
    10621 ctcccatcaa tggaactatt ttaacacaaa ataaacgcaa accccaccac ttaagagaga
    10681 ttgccgatct ttttgtataa tctttataat agaaagaaac ggagtcttat tcttgacttg
    10741 cgcacggata cataaaagga tagactgttg tcatgaaaga aatgagaagg atggaagaca
    10801 tgacaaatca ttactattct gaacgcccag atacggcgca cgattttgaa caatgggctt
    10861 ttgagttaaa aggcagaacc tttcaatttg taacagattc tggtgtattt tcacgtgaaa
    10921 cagtggatta tggttcacgt gtcctgattg atgctttcga ttgggaagag ttacctgatg
    10981 aaggaaagat cttagatgtg ggatgcgggt atggaccgat tgggttagct ttggcatttg
    11041 cgacacagcg gtttgttgaa atggtcgaca ttaatgaacg agcggtaagt ctagcgcaag
    11101 gaaacgcaaa gcgtaaccaa atcgaacatg ttgatatcca tcaatcgaat atctatgaag
    11161 cagttcatga agaaacctat gcagctatcg taagcaatcc gccgatccgt gctggaaaaa
    11221 aagttgtaca tgaaattttg acaggcgcat atcctcggtt gaagaagggc ggtacactga
    11281 caattgtgat ccagaagaaa caaggagcgc cgagtgcgca aaagaaaatg gaagaaacat
    11341 ttggtaatgc agaaatcgtc acaaaagata aaggatatta tatcatcaaa agtgtgaaag
    11401 aataactagc tgaaaaatag tggagcgacg aaagagtgga actgggcgtg catgtacggg
    11461 tacgtatcag ttcttttttt gttgagaagt ttttaaaaac aaagaactat tttttcttca
    11521 aaaagaaata gtaggaacag agtcgttgtt ttttacgata aaataaataa gatacagaac
    11581 ttgtatagaa ggctgccaag tcggtagata aaggaggttt ttatcaaaaa acatactttc
    11641 cttttttctc tattcttttc ttgggggttt tggtacactg atagggaatt tagatagaag
    11701 ggagccaata ggatgaagct gaccatcaga gatatcgcag aaatggcagg cgtatcagta
    11761 acaactgtct cacagatttt gaataacaaa ggaagcagat ttagtgataa gacaagaaaa
    11821 aaagtgttgt cgatcgtgaa tgaacatcat tataagcccg attatttcgc ttcaaatgtg
    11881 atcaatcgac attcaaagac catcggaatg atcgtccctg atgtcactga tttcttcttc
    11941 tccaaactga tcgaaggtgt agagagctat ttgaatcctt tgggctatgt gatcatgtta
    12001 tgcaattctc gtcatagcca agaaaatgag atcaagtatt tacaagagct ttcccaccgc
    12061 tcagtggacg gaatcttgct ggccacacca aatattttac ctgagaaata tgcattagac
    12121 agcggttttt atcaaaaaat gccaatcatt ttaatcgatc gcggattgaa tcgtcgggat
    12181 aacggacggc ttatcgtaaa ggaatatgag ggcgcctatc aagctgtttc gctgtttatt
    12241 gaaaatggcc atacgaagat cggcatgctg aaggaaacca ctggatacta tcaattagaa
    12301 gaacgtttca acggatatcg tcatgcgctg aaagatcatg gcctgccttt taatgggaaa
    12361 tttgttgaac atggagagtt gactgtccaa ggtggatata ctgcatcaaa aaaattactc
    12421 aaacataaag atatcacggc tattttttgc gggaatgatg ccatggcgat cggatgctat
    12481 caggcgatcg atgaattggg caaaaaaatc ccagaagata tctcagtcat aggattcgat
    12541 ggattaaagc tgtcagaata catgattccg caattgacga cagtgcagca gccaagtttt
    12601 gatattggat tttatgctgc acgtttcctg atcgatacga tcgaatttcc tcaaagaaaa
    12661 gttccgaaca aagtgttcga aacaaaatta attattcgtg agagcgtaaa agtattgaca
    12721 aagggctaac tcggcggtat attgcttatg taagcattta caaaggctag ttagtgcttt
    12781 ttttattttt agtaaaccag tttactaaag tggtttacct ttcctaaaaa taatgtataa
    12841 taccgaatga agacaaggag gatcaggcaa tgcgaatggt ggatctgatt gaaaaaaaga
    12901 gggatggaaa cgagctgtcg aaagaggaaa tcgagtacat cgtcacgaac tatacgaatg
    12961 gaaagattcc agattatcaa gtcagtgcat tattgatggc aattttttat caagacatga
    13021 caaatgaaga aatcacgaac ttaacattgg cgattgctaa ttctggtgat gtcatcgatt
    13081 tgagttctct tgaaggaata aaggtagata agcattcgac aggtggcgtt ggtgacacga
    13141 ccacattgat tttagccccc ctagtagcta gtgtcggagt gacagtggct aaaatgtctg
    13201 gacgcggtct aggttacaca ggtggtacac tagacaagct agaagcaatt cccggatttc
    13261 agatcgagct ttcggatgaa gaatttgtac gcattgttaa tgaaagtaaa gtagcggtaa
    13321 ttggtcaatc aggaaacttg gcaccggcgg acaaaaaatt gtatgccttg cgtgacgtca
    13381 cagcgaccgt tgattctctt ccgctgattg ccagttcgat tatgagtaaa aaaatcgcgg
    13441 caggagcaga tgcaatcgtt ttggatgtga ccactggaga cggtgctttc atgaagaaca
    13501 tcgaagatgc aagacgtctg gcaaagacca tgacgagcat tgggaaactg gctaaccgag
    13561 aaacagtggc ggttatctca gatatgtcag aacctcttgg cgaagctatc ggaaatagtc
    13621 tagaagttgt cgaggcaatc gaaacattac aaggaaatgg tccagaagat ctagtagaaa
    13681 tgtgctacgc cttaggaagc caaatggttg ttcttgctgg caaagctaag acaaccgatg
    13741 aagcaagaac gttgcttcaa gaagctctgg aatcaggtaa agcgttagca aaattcaaag
    13801 aaatgatcca aaatcaaggg ggagacccag cgatcgtgga acatccagaa cgaattttga
    13861 ctgcacgcta cacaatggag cttcctgcta aacaaagcgg agttgtgtca aaaatcgtgg
    13921 caaatgaatt aggaattgct gcaatgatgc taggtgctgg gcgtaaaacc aaagaagaag
    13981 atattgatca cgctgttgga ttgaaactgc ataaaaaaat tggtgacact gtgacaaaag
    14041 gtgagtcact attgacgatc tatagtaacg acgaagaaat cacttctgtc attgaattat
    14101 tatataaaaa tattgaaatt ggtgaatcgg caatgaagcc aacgttgatt catgacatca
    14161 tcacagaata aaggaaaaaa gcggacttta ccgttttaaa gaaaagggga aaagaaatgg
    14221 aactaaaccg aatgatcgac catactattt tgaaagcaga cgcatctaaa gaagctgtga
    14281 tgcaaattat cgaagaagca aaaaaatacc atttttattc tgtctgcatc aatccagcat
    14341 gggttgcgct agcgaaagaa caattacaag gtgagccagt agccgtatgt acagtaatcg
    14401 ggtttcctct aggagcaaac acacccgaga caaaagcatt tgaaacaaca gatgccatca
    14461 ataacggtgc agatgaagtc gatatggtca tcaatatcgg tgctttgaaa tcaaaggatg
    14521 atcaacaagt ccaaaaagat atcgaagcag ttgttgaagc agcaaaagat cgtgcgcttg
    14581 tcaaagttat tattgaaaca tcgttattag atagagaaga aatcatccga gcttgcgaaa
    14641 tagcaaaagc tgcaggcgct gattttgtca aaacatcaac tggtttctca acaggcggag
    14701 caaaggtaga ggatgtccgt ttgatgcgtg aaactgtagg accagaaatg ggtgtcaaag
    14761 cttccggcgg tatccataac gaagaagaag caatggcaat gatcgaagct ggtgctacaa
    14821 gaatcggaac aagcgcagga gtagcaatcg tatctggttc aactggtgaa ggctattaat
    14881 tttttcaaag gagaagctca tggcagaact caaacaagaa tggatcaaca cagctattga
    14941 agcgttagat aaagcctatg ttccttattc ccattttccg gtaggcgctt gtctcgtaac
    15001 ggaatcaggc aagatttatc aaggcatcaa cattgagaat gcatcatttg gattaacgaa
    15061 ttgtgccgag cgcacagctt ttttcaaagc agtatctgaa ggagaaaaaa catttacgca
    15121 tctagtcgta gcggggcaca cccccgatcc tatctctcct tgtggtgcat gtcgacaagt
    15181 aatggcagaa ttctgttccc ctgatatgcc ggttacgcta gtaggagaca atggtgtgac
    15241 gaaggcaacg accgttcgtg cactgctgcc ttatgcattt actgaaaaag atctataact
    15301 catgttcatt cagtcagctg actgttgaaa aataaaacaa ttattttagg aggctttaca
    15361 gaaatgaaaa aagcaaaatt atttggttta ggtgctgtcg cactagcagc agggttattt
    15421 ttaggtgcat gcggcaacaa cggctctact gattcatccg gtggcaaaag ctcatcagac
    15481 acaacaactg cagcattgat cacagacaca ggtggtgtag acgaccgttc attcaaccag
    15541 tcagcttggg aaggactaga aaaatggggg aaagatcaag gactttcacg aggaaatgat
    15601 ggcttccaat atttccaatc ttcaaatgaa tcagattaca tcccaaatat tgaccaagca
    15661 ttgaatgctg gattcaaaac gatttttggt attggttata aactaaaacc agcgatccaa
    15721 gaacaagcaa caaacaatac aggtacgaac tttgttatta tcgatgatgt catcgatgga
    15781 ttagataacg ttgtatctgc aactttcaaa gacaacgaag cttcttatct tgcaggtgtt
    15841 gcagcagctt atacgactga aacaaacgta gtcggcttta tcggcggtgt aaaaggtgaa
    15901 gtaatcgatc gttttgatgc tggattcaaa gccggtgttg atgcaggtgc gaaagaatta
    15961 ggaaaagaaa tcaaagtatt gaatcaatat gctggtgact tctctgctcc agataaagga
    16021 cgttcaattg ctcaaggtat gtatgcacaa aacgcagata tcatcttcca tgcttcaggc
    16081 ggtacaggta acggtgtatt ccaagaagca aaatcattga acgaatcagg cgacaaaaaa
    16141 gtttgggtta tcggtgttga ccgcgaccaa tcagacgaag gcgaatacac attaaacggc
    16201 gaaaagaaaa acttcacatt gacatctaca ctgaaagcag ttggaacagt agtagaagat
    16261 ttggcacaaa aatcagcgga cggtaaattc cctggcggcg aacacactgt ttatggattg
    16321 aaagaagacg gtgttggtct aacagaagga caactttctg acgaagcgaa aaaagcagtt
    16381 gatgaagcaa aagaaaaaat catttcaggt gacgtaaaag ttccagaaac tcctgaagaa
    16441 aactaaaacc atttctatag ccttgttaga cacatgtttg acaaggctat ggtttgatta
    16501 gaacaagaat tgttcattta tccaagctgc cttgtgacag tttggataag ggaacaactc
    16561 cctgaagtaa tgtttgtttc aaaagaagaa cgctcactaa gcgtgaatct tagtcaatcg
    16621 ttgccctaga tagaaatcgt taggatgtga aaaaaaggtg gttcaagaaa atattgtcat
    16681 agaaatgcag aatattacca aagaatttgg aacattcaag gcaaatgaca acatcaattt
    16741 acaagtaaag gcaggagaaa ttcatgcgtt gcttggagaa aacggtgctg gcaaatctac
    16801 attgatgaac gtgctttccg gattattaga gccgacatca gggaaaattt tgatgcgtgg
    16861 gaaagaagta aagatcacaa gtccgaccaa agccaaccaa ttagggattg ggatggtcca
    16921 tcagcacttt atgcttgttg atgcctttac tgtaacagaa aacatcgtgt tgggaagcga
    16981 gcctagtcgc gcagggatgc ttgaccataa aaaagcacga aaagagatcc aaaaagtttc
    17041 tgaacagtat ggattgtcag tcaacccaga tgcttacgtt cgtgatattt cagttggaat
    17101 ggaacaacgg gtagaaattt taaaaacact ttaccgagga gcagatgtac tgatttttga
    17161 tgagccgaca gctgtattga cccctcaaga aattgatgaa ttaatcgtga tcatgaagga
    17221 attagtcaaa gaaggcaagt cgatcatttt gattacgcat aagttagatg aaatcaaagc
    17281 agtagctgac cgctgtacag ttatccgccg tggaaaagga atcggtacag tcaacgttaa
    17341 agacgtaacc tcacaacaat tagccgatat gatggttgga agaacggttt cattcaaaac
    17401 gatgaaaaaa gaagcgaagc ctcaagaagt cgttttgtct attgaaaatc tagtggtgaa
    17461 agaaaatcgt ggattagaag ccgtgaaaaa cttgaactta gaggttcgtg ctggcgaagt
    17521 acttggtatc gctggaatcg atggaaacgg gcagtcggag ttgatccaag ctttgactgg
    17581 tttgcgaaag gcagaaagcg gacatatcaa gctaaaaggg gaagacatca ctaataaaaa
    17641 accccgaaaa atcactgaac atggtgtagg acacgtgcca gaagaccgtc ataaatacgg
    17701 gttagtccta gatatgacat tatctgaaaa tatcgcacta caaacctatc atcaaaaacc
    17761 ttacagtaaa aacggcatgc tgaattattc agtaatgaat gaacatgcca gagaattgat
    17821 cgaagaatat gatgttcgaa caacgaatga acttgttccc gcaaaagctt tatcaggcgg
    17881 aaatcagcaa aaagcaatta tcgctcgtga agtcgaccga gatcctgatc tgttgattgt
    17941 tgcaaatcca actcgtggac tggatgtagg tgcgatcgaa tttattcata aacgtctgat
    18001 cgaacaaagg gacaaataca aagcagtgtt actgatcagt ttcgaattag aagaaatttt
    18061 aaatgtttcg gatcgtattg ctgttatcca tgaaggagaa attgtcggta tcgttgatcc
    18121 gaaagaaaca tctgaaaatg aattaggtct attgatggcg ggctatacct tagaagaagc
    18181 acgaaaagag ttagaaaagg gggctggcgc acatgaatga tcgcagcgaa aaattccgta
    18241 atttattagt gcctgtactt tcagttgttt taggattgat cttaggcgcg atattgatgg
    18301 ctgcttttgg ttttcagccc atccaaggtt attcttctat gctagatgca gcccttggtt
    18361 cgcaaagagg gataggtgaa acgttaaggc aagcaacgcc gttgattttt acagcattag
    18421 gtttttctgt agctaactca gctgggtttt tcaatattgg gctttctgga caagcacttt
    18481 gcgggtggat cgtcagtgtc tgggtggcac ttgcgtttcc agatctgcca aaagttgttt
    18541 tgcttccgtt atgcgtgatc ttaggtgcct tagctggtgc gatggcagcg gctgttccag
    18601 gtttacttcg agcatttttt ggaacaagtg aagtgatcgt aacaatcatg atgaactata
    18661 tcttattata cgtaagcaca tttgctttac atgaagtgat gcctgaaagc tatcgctcaa
    18721 gtttggactc ttccaatatg atcagtcaaa atgcctcact gcggttacca tggttaacac
    18781 aagcttttgg cggctctcgc gtaaactcag gattattttt agcaatcatc gcgcttattt
    18841 tcgtttggct gctgatgaag aaaacgactt taggatttga aatccgttct gttggattga
    18901 atccttttgc ttctgagtat gcaggaatga gtagtaaacg tacgatcgtt ttgtcgatgg
    18961 tcatttctgg cgctttggca gggcttggcg gtgtggttga aggattagga acgtatcaaa
    19021 acttctttgt tcaaacgact tcgctatcta ttggatttga cggaatggct gtttccttgc
    19081 ttggttctgg atcagcaatc ggtattcttt tatcagcgat cttgttcagt attttgaaga
    19141 tcggtggact tggcatgcag acaggagcag gtgttccgtt tgagatcgtc aatgtatcga
    19201 ttgctttgat tatcttcttt gtcgggatca acttcctgat tcgtttcgtc atggctaaag
    19261 tcttccaagg gaaaaaacaa gaagagatcg ttgcgacaat agaatcgaaa ccaacgaatc
    19321 aaaaccatga agggggcgaa ctataatgtc gactatcgaa ttaattgcct tgatccttac
    19381 ttctacactg gtttattcaa caccgctaat tctaacgtca ctaggcggaa ctttttctga
    19441 acgaagcggg atcgtaaacg tagggctaga gggaatcatg gtcatgggtg cttttagttc
    19501 catcgttttc aacttgacga tgagtccatc acttggttct atgacaccat ggatcgctat
    19561 tttagtgggt ggcgtcgtag gtatcctatt ttctctgatt cacgcagtag caacagtcag
    19621 tttgcgtgct gaccatatta tcagcgggac agtaattaat ctgatggcac ctgctttagg
    19681 tgttttcttg atcaaagcat ggtatggtaa aggtcaaact gataacatca cgcagaatct
    19741 ggggtatttt tctttcccag gattagcaga tattccggtc attggaccaa ttttctttaa
    19801 aaatacattc ttacctgctt attgtgctat tttgattgct gtcctagctt gggtgatcat
    19861 cttcaagacg aaattcggct tgcgcctccg ttcagttggt gaaaatcctc aagctgccga
    19921 cacattaggt atcaatgtat atgctatgcg ttattcaggc gtactgattt ctggcttgtt
    19981 aggcggtatg ggcggtgctg tttttgctca gtcgatttcc ggtaactttt cagctggtac
    20041 gatcgttgga caagggttca tctccatggc tgctatgatc tttggtaagt ggaatccttt
    20101 aggggcaatg ggtgcctcat tgttctttgg ttttgctcaa agcttgagta tcattggcgc
    20161 acaattacca gttatttctt cgattccgcc agtattgctg caaattgctc catatttgct
    20221 gacaatcatt gtgctggttg ttttcttagg gaaagcttct ggtccaaaag caaacgggaa
    20281 gaactatatc aaatcaaaat aatcttttag cgaataaaaa gggtgggtta cgggaagtgt
    20341 ttttcgtctc gccctttttg ttttaaaggc aataaaaata aaaagaaaga aggaataatc
    20401 atgtttaaac gcgtacatct gatcgtcatg gattcagtag gaattggaga agcaccagat
    20461 gccgataagt tcggtgatgt tggttctgat acattaggac acattgcgaa agaagcagga
    20521 ttgaacgttc cacatttaga gcagcttggt ttaggaacga tccgtcctct tgaaggtgtg
    20581 gaatcagtga aagatcatca aggctatgcg acgaaattag aagaagtttc tgtagggaaa
    20641 gatacgatga caggccactg ggaaatcatg ggattgaata tcaaaactcc atttcgtgtg
    20701 tttcctaatg gattccctga tgatctgatc aaacaaatcg aagaattttc tggccgtaaa
    20761 gtcgtatgta acaaacctta tagcggaaca gctgttattg acgattacgg tgaacaccaa
    20821 atgaaaacag gcgatttaat tgtatatact tctgccgatc ctgtccttca gatcgcggca
    20881 catgaagaaa tcattccttt agaagaatta tatcggattt gccaattcgt tcgtgatata
    20941 acaaaagatg atccgtatat gattggccga atcattgcac gtccgtacgt gggtgaacca
    21001 ggagacttca cacgaacaag caatcgccat gattatgcgt tgaatccatt cggacacaca
    21061 gtccttgatt ctttgaaaga agcaggaaag gacgtgattg ctgttggaaa gatcaacgat
    21121 attttcaacg gacaaggaat cactgaatct gtccgcacga aaagcaatat ggatggcgtt
    21181 gaccagctgc tgaaagttat gaaaaacgat ttttcaggaa tcagctttac caatttagta
    21241 gattttgatg cattatacgg tcatcgtcgg gatgtagtcg gctatgcgca tgcgattgaa
    21301 gaatttgact tacgaattcc agaattgctt gaagcgatgg aagaagacga cttgctaatg
    21361 attacagccg atcacggaaa cgatccgacg tttactggta ccgatcatac gcgagaatat
    21421 gtaccgttat tggtttacag taaaaaaatg aaagaacaag gggcattgcc tcaaggatat
    21481 tattcagata tttcagcaac gattgctgag aactttagtg tttcttcgac tgaaaatggc
    21541 gaaagcttct tgaaattatt aaaataatct ttttaaatag gagaaagcaa atgaccaagt
    21601 taagcgagat gttacaagaa acgacccagt ttatccttga taaaggagta aaagaaattg
    21661 attttggttt gattctaggt tcaggattag gtgaattagc agatgaaatc gaagatgctg
    21721 taatgatttc ttatgatgag atcccgttct ttcctgtttc gacagtagtt gggcatgcag
    21781 gaaagctagt ttatgggtct ctggcaggca agaaagtgtt agccatgcaa gggagattcc
    21841 actactatga aggtcattcc atgcaaacag tgacctatcc agtacgtgtg atggccggac
    21901 taggggccca ttcagtcatt gtgactaatg catgtggtgg tgtcaacgaa tcatttgtac
    21961 caggagattt gatgctgatt accgatcata tcaattttac tggtgaaaat cctttgatcg
    22021 gtccgaatga agatgcaatg ggccctagat ttccagatat gagtcaagct tatacgcctg
    22081 actatcaagc aacagcaaaa gcagcagcag aaaaactagg tttggatctt aaagaaggcg
    22141 tatatatggg ctattctgga ccaacttatg aaacgcctgc ggagatccga atgtctcgga
    22201 caataggagc cgatgcagta gggatgtcga ctgttccaga agtcatcgta gcagctcaca
    22261 gcggattaaa agtgttagga atctcttgta ttaccaactt agcagcaggt atgcaagcga
    22321 atctgagcca tgaagaagtt gtagaaacaa ctcagagagt caaacaatct tttaaagcat
    22381 tggttaaaga aacattgaca ttattgtaat aggaggaaca ataaaatgag tgtgcatatt
    22441 gaagcgaaag aaggcgagat cgccgataag attttacttc cgggagatcc tttgcgagct
    22501 aaatatattg cggagacttt tttggaagat ccagtttgtt acaatcaggt acgcggtatg
    22561 ttaggcttta ccggcaaata taaaggagaa cgtatttctg ttcaaggaac aggaatggga
    22621 atgccatcag ccaccatcta tgcccatgaa ttgatccagt cgtatggtgt aaagaaattg
    22681 attcgagtag gtacatgcgg tgcattgtca aaagacgtcc atgttcgtga tttagtacta
    22741 gcacaaggtg cagcgaccag ttcttcgatg atcgaaaaaa atttccaagc ctttcatttt
    22801 cctccaatca gtgatttcaa tctattactg aaagcatatg agatagctaa agaaaagggc
    22861 tatacggttc atgtaggaaa tgttctttcg gaagattctt tctacaaaga tgatttgaca
    22921 gagacgttcc aattagctga acttggcgtt ttaggagtgg aaatggaagc agcggctctt
    22981 tattacttag gcgcgaaata ccatgtgcag acattaagtt tgatgaccgt tagcgaccat
    23041 ttgatcaccg gtgaagaaac aacagctgca gaacgtcaat ctacgttcaa tgaaatgatc
    23101 gaagtaggat tggaaacagc gatagcagaa taagaaggaa atgaaaaagc tgccctgcat
    23161 taagtaataa gaacatccaa gcaaaaatag ctcctcatgt ttttgacttg gctggactta
    23221 ttattgcaaa tgtaagcttt tgaacaccgt ttaccaagag gttgtgaatc tgccgtttaa
    23281 ctctgagtca caaggaacaa tttcaaaaaa cagcttttca tgtttgatga atattgtgac
    23341 ttgtgaccga gaagttggca ggagaaaacc gtttatacga aaaaagggac tgtgacaaat
    23401 cgtgtcacag tccctttttt atagtgtttt ttataggtag tattttttag caactgttaa
    23461 gtcatcattt aattcataaa caagtggttg acctgttgga atttcaagat ccatgatgtc
    23521 ttcgtcagag ataccttcga tatgtttagc tagggcgcgt aatgagttac catgtgcagc
    23581 tactaaaaca gttttgttgt ctagtaatgc tggagcaatt tcatcttgcc agaatggcaa
    23641 agcgcgttct aatgtcactt tcaagttttc tccaccagga atatcgcgtt tgtctaacat
    23701 tgcgtaacga cggtcatttg ctgcagaacc ttcgtcagtt tcttccatta atggaggaag
    23761 agtatcgtaa gaacgacgcc agatgtgcac ttgttcgtcg ccgtattttt cagctgtttc
    23821 ttttttgttc aaaccttgta atttaccata gtgacgttcg tttaagcgcc atgatttgat
    23881 ttgtggtacc caaagttgat ctgaatattc taagatcaag ttacatgttt tgattgcacg
    23941 agttaaaaca gaagtgtaag caacatcaaa ttcgattcca gcttctttga ttttgcgtcc
    24001 gccttctttt gcttcttcga ttccttctgg tgctagattt acatcagccc aaccagtaaa
    24061 ttggttcaat gcattccatt cactaagacc gtgacgagaa aaaactaatt ttggcatgtt
    24121 tagctctcct tttaagatag gtttatattt ctgaaatttt cagttctgtt ttattgtaca
    24181 attttttgga agagatttcc atcgaaaaat tagataagat tctatgagaa aacaagtaga
    24241 gaatataaaa aaacgaacag tgcccacgca actgttcgtt tttaggtatt agatgcataa
    24301 ttgctcgatt tctttttcag gtaaattgat atatttataa catgttccaa ctgaaacacc
    24361 gcattttgaa gaaataaatt ggatcgtttc ttttttttca taatagagac gtcggatttt
    24421 tttgatcgtt tttgcatcga tttttggtct tccgccgatt tttccttttt tacgagcttc
    24481 atctaatcca actaatgtac gttctttgat caatgcgcac tccatgtttg ctaggttttc
    24541 cattaattgg aaatagattt ttcccatttc gcgcgtatcg attccttcat caagactaat
    24601 caagtgcagc ccgcgatctt tgaataattg cgtgagttct gttaattgcc gcgtagattt
    24661 gcctaatcga tgaagttggc aaatgactag tgcatcgcct tgttgcatgt cagcgatcac
    24721 tgactccaaa tgaacagcag tagaatcatt ttgttccact tcatatgtct catggaaaat
    24781 ttcttgacaa ccaaagtcag ataattgatc aacctgagtt gttaaatcaa cgtcatttat
    24841 ggttgtgcgt gcataaccaa taattttcat ggtgattcct ttctagcagt gattttctaa
    24901 ctggatataa cagtaaaaat gtagcataaa gtacgtcaaa aagatagttt tttcgcaaat
    24961 tttccgctgt ctttttttat tggatgatca agcagttttt ctttatttac gacggattgc
    25021 gtcaagaatc tcaacgcctt tttctgagcc gacaactact ttagaaacca tgtttaggaa
    25081 aagcccatgt tctactaccc caacaagttc atctaaatag gtagctaagc tttctggctg
    25141 ttcgatgact tctagatgca gatcaatgat gtaatgacca ccatctgtgg ttaagacttc
    25201 tccgttttcg tctgtacgca agacgggttg gaatcttttt tcttcaaaaa tatgaagtag
    25261 ctgttgggag ccataaggga tcacttcaac tgggagtggg aacttcccaa gtttatcgac
    25321 taatttagaa gaatcaacga tccaaatcgt ttcttttgag tacgtagcga ctattttctc
    25381 gaaaagcaaa gcagcgccgc cgcctttgat cccttgaaaa tcagcactga tttcatcagc
    25441 accgtctatt gtcaaatcga ctacggggac ttcatcaata ctctttaacg gtattcctaa
    25501 tttttccgct tgttcttttg tcgcatttga agtagtaact cctgtgatac gtaaaccttc
    25561 ttcacgcata cggcgaccaa tctcttctac catataatag gcggttgaac cggtcccgag
    25621 acctacgatc atcccgtcct ttacgaattt cgcggcttca atgccagcta attgttttaa
    25681 attcatcttt gcgtccacct ttttttagtt tgagcatcgt gctttcctca ctattttagc
    25741 gtgatttacg gtaaaaagat accattcttt tttattccaa taaagctttt tcaaaaaaaa
    25801 ttgcatctct tgttgacatg ttttttcatt cgtgatattc taacaaaggt gctttatcta
    25861 caggtttctg tgaagaaacg tgctgactgt ttatcaaagc atatttgata aggatgcaga
    25921 cattgcattt attttttatc acaaaaaaag aaccacctgg atgtgtggaa ctagacgcaa
    25981 atcgtgggaa ggaggaaaca aggaatgcct acaattaacc aattagtacg taaacctcgt
    26041 aaatcaaagg tggaaaaatc taattcacct gctttgaaca aaggatataa cagctttaag
    26101 aaaactcaaa ctaacgtaaa ctcacctcaa aaacgtgggg tagcaactcg tgtgggtaca
    26161 atgacaccta aaaaaccgaa ctcagcttta cgtaaatatg cacgtgttcg tttgtcaaac
    26221 ttaattgaag ttactgctta tatcccaggg attggtcata acttacaaga acacagtgtt
    26281 gtattattac gcggtggtcg tgtaaaagac ttaccagggg tacgttacca tatcgttcgc
    26341 ggtgctttag atactgctgg tgttaacgac cgtaaacaaa gccgttctaa atacggaact
    26401 aaaaaaccta aagcttaatt ttaattatta aacaaatgaa acttgcttct actatataga
    26461 agtaattact atgaacaata tttcggaagg aggagttacg gatgccacgt aaaggtcctg
    26521 ttgcaaaacg tgatgtttta ccagatccta tttataactc aaaattagta actcgcttga
    26581 tcaaccgtgt aatggttgac ggaaaacgcg ggattgcagc taacattatc tataatgcct
    26641 ttgacttgat caaagaatct acaggtaacg atccattaga agtgtttgaa caagcaatga
    26701 aaaacgttca acctgttctt gaagttaaag cacgtcgtgt tgggggttca aactaccaag
    26761 taccagttga agttcgccct gaacgccgcg ttactctagg tcttcgttgg gtagtaaact
    26821 acgctcgctt gcgcggtgaa cacacaatgg aagaacgctt agcaaaagaa atcatggatg
    26881 ctgcaaacaa cacaggtgct tctgttaaaa aacgtgaaga cactcataaa atggctgatg
    26941 ccaaccgtgc gtttgctcac tatcgctggt aagattctct ctgtttggtc ggcttttata
    27001 agtcgacctc agatagtgtc cttattttta gcaactagat agtaaagaga ggagaaacaa
    27061 ggagaatgac cagagaattt tctttacaaa acactcgtaa tatcggtatc atggcccaca
    27121 tcgatgctgg taaaacgact gctactgaac gtatcttgta ctatactggt aaaatccata
    27181 aaattggtga aactcatgaa ggagcttcac aaatggactg gatggaacaa gaacaagaac
    27241 gtggtattac gatcacttct gctgctacta cagcacaatg gaagggtcac cgaatcaaca
    27301 tcatcgacac accaggacac gtagacttca cagttgaagt tgaacgttca ttgcgtgtac
    27361 ttgatggtgc tgtaactgtt cttgatgcac aatcaggtgt tgaacctcaa actgaaacag
    27421 tttggcgtca agcaacaact tatggtgtac ctcgtatcgt atttgcaaac aaaatggaca
    27481 agatcggtgc tgacttctta tattcagtat caactttaca tgaccgtttg caagcaaacg
    27541 ctcacccaat ccaattacca attggtgcgg aagacgactt cacaggaatt atcgaccttg
    27601 taacaatgaa ggctgaaatg tatacaaacg atcttggaac agagatcgaa gaaacagaaa
    27661 ttcctgaaga ataccgcgaa ttagctgaag aatggcgcga aaaattggtt gaagctgtag
    27721 ctgaaactga tgaagaactt actttgaaat acttagaagg tgaagaaatc actgaagctg
    27781 agttaaaaga aggtatccgc cgcgcaacag ttaacgtaga attctaccca gtactatgtg
    27841 gttcagcatt taagaacaaa ggggttcaat tactattgga cgctgttctt gattacttgc
    27901 catcaccgtt agatatccca gctatcaaag ggattgaccc taaaactgat gaagaagtag
    27961 aacgtcctgc agacgacagc gctcctttct cagctttagc atttaaagtt atgactgacc
    28021 cattcgtagg tcgtttaact ttcttccgtg tgtactctgg tgttttacaa tcaggttcat
    28081 acgtacaaaa cgcaacaaaa ggcaaacgtg aacgtgttgg tcgtatccta cagatgcatg
    28141 ccaactctcg ttcagaaatc tctgaagttt acgctggaga tatcgctgct gctgttggtt
    28201 tgaaagacac aactactggg gacacactat gtgatgaaaa gagtctagtt attcttgaat
    28261 ccatggaatt cccagaacca gttatccaag tcgctattga acctaaatca aaagctgacc
    28321 aagataaaat gggtgtggct ttacaaaaac tatctgaaga agatcctact ttccgtgctg
    28381 aaacaaacgt ggaaacaggc gaaacaatca tcgctggtat gggtgaactt cacttagata
    28441 tcatcgttga ccgtatgcgt cgtgaattca aagttgaagc taacgtaggt gcgcctcaag
    28501 tatcttaccg tgaaactttc cgtgctggca ctcaagccga aggtaagttt gtacgtcagt
    28561 ctggtggtaa aggtcaatac ggtcacgtat ggattgaatt tacaccaaat gaagaaggtg
    28621 ctggtttcga atttgaaaat gcgatcgtcg gtggggttgt tcctcgtgaa tacattccag
    28681 ctgttgaaac tggtttgaaa gatgctatgg aaaatggtgt cttagctggc tatccattag
    28741 tagatatcaa agctaaactt tacgatggtt cataccatga tgtcgattca aatgaaactg
    28801 ccttccgtgt tgctgcttct atggcattac gtgcagcagc taagaaagct aatccagtaa
    28861 tccttgaacc aatcatggct gtagaagttg ttatccctga agattattta ggtgatgtta
    28921 tgggtcacgt aactgctcgt cgtggacgtg ttgaaggtat ggaagctcgc gctggcggcc
    28981 aacaagttgt tcgtgcgatg gttccacttg ctgaaatgtt tggttatgct acaactcttc
    29041 gttctgctac tcaaggtcgt ggtacattta caatgacatt tgaccactac gaagatgtac
    29101 caaaatcagt acaagaagaa atcattaaga aaaacggcgg aaaagctgaa taatttcttg
    29161 aaaccggata gtcctgcaaa gagattatcc aaagagacta tatttttaat atagtttcgt
    29221 gtaaaataat tatcgatgat atgggcatgt ggtgtacctt gtgtacatca tatacctatc
    29281 agacaaataa aaaatatatt gtatttttag gaggacattt taaaatggca aaagaaaaat
    29341 ttgaccgttc taaaccacac gttaacattg gtactatcgg acacgttgac catggtaaaa
    29401 ctacattaac agctgcaatc acaactgtac tatctaagaa aaacggcggc caagctatgg
    29461 cttacgatca aatcgatggt gctccagaag aacgcgaacg cggaatcaca atctcaacag
    29521 ctcacgttga gtatgaaaca gatacacgcc actacgctca cgttgactgc ccaggacacg
    29581 cggattacgt taaaaacatg atcactggtg ctgctcaaat ggacggagct atcttggtag
    29641 tttctgctgc tgacggccca atgcctcaaa ctcgtgaaca catcctattg tctcgtcaag
    29701 ttggtgttcc ttacatcgtt gtattcttga acaaagtaga catggttgat gacgaagaat
    29761 tactagaatt agttgaaatg gaagttcgtg acctattaac agaatacgaa ttccctggtg
    29821 acgatgttcc tgtagttgct ggatcagctt tgaaagctct agaaggcgac gcttcatacg
    29881 aagaaaaaat tcttgaatta atggctgcag ttgacgaata catcccaact ccagaacgtg
    29941 acaacgacaa accattcatg atgccagttg aagacgtgtt ctcaattact ggacgtggta
    30001 ctgttgctac aggtcgtgtt gaacgtggac aagttcgcgt tggtgacgaa gttgaagttg
    30061 ttggtattgc tgaagaaact tcaaaaacaa cagttactgg tgttgaaatg ttccgtaaat
    30121 tgttagacta cgctgaagct ggagacaaca ttggtgcttt actacgtggt gttgcacgtg
    30181 aagacatcca acgtggacaa gttttagcta aaccaggtac aatcacacct catacaaaat
    30241 tctctgcaga agtatacgtg ttgacaaaag aagaaggtgg acgtcatact ccattcttca
    30301 ctaactaccg tccacaattc tacttccgta caactgacgt aacaggtgtt gttgaattac
    30361 cagaaggaac tgaaatggtt atgcctggcg acaacgtaac aatggaagtt gaattaatcc
    30421 acccaatcgc tatcgaaaac ggtactaaat tctcaatccg tgaaggcgga cgtacagttg
    30481 gtgccggtgt cgttacagaa atcaaagctt aattcactta agttttaaag taaagcaaca
    30541 tttattcgga cgtaatcgct cggaacgaag gtcttaggat ctttgttccg agctttttta
    30601 tttattgcag gatcaggaaa aaagacagga gtattataaa tagaagaaac acgaaataaa
    30661 agagtcagta ttttctaaat gaaactttat gaaaacaaaa tgttttttac aagaaaaagc
    30721 cttgctttgt gagtttatat atattataat gacaaaggtg cgtttacttt agtgaattgc
    30781 acagacgtaa tcagaatgat ttcgtcggct aagaaacgag aggttgcgac acgcccggac
    30841 gctttgccac gaacgagcgt gacggaaatt ttcgtggagc tatgtctact tttcaaaata
    30901 ggcgaaggag ggaacaagat ggcaaaacaa aaaattcgta tccgtttaaa agcgtatgaa
    30961 caccgtattt tagatcaatc agcggataaa atcgtggaaa ctgcaaaaag aactggagct
    31021 agcgtgtctg gtccaattcc attaccaact gaacgcagtc tttacacaat catccgtgcg
    31081 actcataaat acaaagattc acgcgaacaa ttcgaaatgc gtacacacaa acgtcttatc
    31141 gacattgtga acccaacacc aaaaactgtt gatgctttga tgaagctaga cttaccatct
    31201 ggtgtaaaca tcgaaatcaa attataaaac taaaacaaaa ataatggagg tgtactcatg
    31261 accaaaggaa tcttagggaa aaaagtggga atgacacaaa tcttcactga atctggtgaa
    31321 ttaattccag taactgttgt agaagctact ccaaacgtag tactacaagt gaaaacaatg
    31381 gaaacggatg gctacgaagc tattcaagtt ggttaccaag atatgcgtga agttttatca
    31441 aacaaacctg cgaaaggtca tgttgcaaaa gcaaacacgg ctcctaagcg cttcattcgt
    31501 gaattcaaaa atgttgagct aggagaatat gaagtaggaa aagaaattac agtagatgtt
    31561 ttccaagcag gagacattat tgatgtaaca ggtactacga aaggtaaagg attccaaggg
    31621 gttatcaaac gccacggaca aagccgcgga ccaatggctc acggttctcg ttaccatcgt
    31681 cgtcctgggt caatgggtcc agttgcacct aaccgtgtat ttaaaaacaa acgtcttgct
    31741 ggccgtatgg gtggcaaccg cgtaactatc caaaacttgg aagttgtacg tgtcgatgca
    31801 gaaagaaatg ttatcttgat caaaggtaat gttcctggag ctaaaaaatc tttaattact
    31861 atcaaatcag ctgtgaaagc taaataattg taggaagaaa ggaggaacta aggaatgccg
    31921 aatgtagcat tattcaaaca agatggaagc caaaacggtg aaatcacact taacgaagaa
    31981 atcttcggaa ttgaacctaa tgaaagtgtt gtttacgatg caatcgtaat gcaacgtgcg
    32041 tcattaagac aaggaacaca cgcggttaaa aaccgtagcg ctgttcgcgg cggcggacgt
    32101 aaaccatggc gtcaaaaagg aacaggtcgt gcacgtcaag gatctatccg ctcaccacaa
    32161 tggcgtggag gtggcgtagt ctttggacca acaccacgtt cttacagcta caaacttcct
    32221 aaaaaagttc gtcgtttagc aatgaaatct gtattgtcag aaaaagttgc tgaaaacaac
    32281 ttggtagcaa tcgaaggctt gaacttcgac gcaccaaaaa caaaagaatt caaacaagtt
    32341 cttgctaact tatctatcga ctctaaagta ttggttgttc tagaaaaagg taacgacttt
    32401 gctgctttat cagcacgtaa cttaccaaac gtttctgtag taacttctga taatgtgagt
    32461 gtgctagacg ttgtatcaaa tacaaaagtg ttagcaacac aaactgctct tactcaaatt
    32521 gaggaggtgc ttgcataatg aacttactag acgtaatcaa acgcccagtg atcactgaaa
    32581 aatcaatgct tgccatggat gacaagaaat acacttttga agtggacact cgcgcaaaca
    32641 aaactttagt aaaacaagcg gtagaagctg cttttgacgt taaagtcaaa aacgtgaaca
    32701 tcgtgaacgt acgtgcgaaa ttcaaacgta tgggcaaata tgcaggatat acaaaaaaac
    32761 gtcgcaaagc gatcgttaca ttgactgaag attcaaaaga aatccaatta ttcgaagctg
    32821 ctgaataagc actaatagat aactcattaa attaggaggg aaaaacgtgg cgattaaaaa
    32881 gtacaaacct acctcaaatg gtcgtcgtaa catgacaggt tctgattttg ctgaaatcac
    32941 aacatcaaca cctgaaaaat cattgttaca accattgaaa aaccatgctg gacgtaacaa
    33001 caacggccgc atcactgtac gtcatcaagg tggcggacac aaacgccaat atcgcgtgat
    33061 cgacttcaaa cgtaacaaag ataacgttgt tgcaactgtt caaacgatcg aatatgatcc
    33121 aaaccgttca gctaacatcg cgttagtaca ctatgaagat ggagtgaaag cttacatctt
    33181 agcacctaaa ggactagaag tgggtatgaa acttgtttca ggtccagaag cagatatcaa
    33241 agtcggaaat gctcttccat tagaaaacat tccagttggt actgttatcc acaacattga
    33301 aatgaaacct ggtaaaggtg gacaattgat ccgttctgct ggtacaagtg ctcaagtact
    33361 tggtaaagaa ggcaaatacg tattgatccg cttgaactct ggtgaagttc gtatgatctt
    33421 agctgcatgc cgtgctacaa tcggttctgt aggtaacgaa caacacgaat taatcaacat
    33481 cggtaaagct ggacgctctc gttggatgcg taaacgccca actgtacgtg gtagcgtaat
    33541 gaaccctaat gatcacccac acggtggtgg tgaaggtaaa gctcctatcg gacgtaaagc
    33601 accagtttct ccttggggtc aaccagctct tggttacaaa actcgtaaca aaaaagctaa
    33661 atcagacaaa cttattgttc gtcgtcgcaa aaataaataa ttactttgac catgtgtcgc
    33721 acattttgag aggaggttca ccatgggtcg tagtttaaag aaaggacctt ttgtcgatga
    33781 gcatttaatg aaaaaagttg aagctcaagc aggggttgaa aagaaaaaag ttatcaaaac
    33841 ttggtcacgc cgttcaacag ttttcccaag ttttgttgga tataccatcg cagtttatga
    33901 tggacgtaaa catgtaccag tttacatcca agaagacatg gtaggacaca aattaggtga
    33961 atttgcacct acaagaactt atcgtggaca tgctgctgac gataagaaaa cgaaacgcta
    34021 atttgagggg aggataaatc caaatggcag aacaaatcac atcagctaaa gcaactgcaa
    34081 aaacagttcg cacttcacct cgaaaagccc gtttagtaat tgaccttatc agaggtaaaa
    34141 gtgtcgcaga tgcaatttca atcttgaaat tcacaccaaa caaatctgct ggaatcattg
    34201 aaaaagtatt gatgtcagca gttgcgaatg cagaaaataa ctttgactta gatgttgaaa
    34261 acttggtagt aagcgaagct tttgttaacg aaggacctac aatgaaacgt ttccgtcctc
    34321 gtgcaaaagg atcagcttct ccaatcaaca aacgtacaag tcatatcacg gtagtcgtat
    34381 cagaaaaata aggagggata atcagtgggt caaaaagtac atccaattgg aatgcgtgta
    34441 ggcatcatcc gcgactggga tgctaaatgg tatgctgaaa aagagtatgc tgagttctta
    34501 cacgaagatt taagaatccg taaatttatt tcaactaaac ttgctgatgc tgctgtatca
    34561 acagttgaaa tcgaacgcgc aacaggtcgc gtaaacattt caattcacac agctaaacca
    34621 ggtatggtta ttggtaaagg cggttctgaa gtcgaaagcc taagaaaaga attaaacaaa
    34681 ttaactggta aaagaatcca tatcaatatc gtggaaatca aaaaaccaga tctagatgca
    34741 aaattagtag gtgaaggaat cgcacgtcaa ttagaaaacc gtgttgcttt ccgtcgcgct
    34801 caaaaacaag ccatccaacg ctcaatgcgt gctggcgctc aagggatcaa aactcaagta
    34861 tctggtcgtc taaacggagc agatatcgct cgttcagaag gatactcaga aggaactgtt
    34921 cctcttcata ctttacgtgc ggacattgat tacgcatggg aagaagcaga tacaacatac
    34981 ggaaaactag gagttaaagt gtggatctat cgtggagaaa ttcttccaac taaaaaaaac
    35041 actgagaaag gagggaaata atcatgttag tacctaaacg tgtaaaacac cgtcgtgaat
    35101 tccgcggaaa aatgcgcggg gaagctaaag gcggaaaaga agtagcattc ggtgaatacg
    35161 gtttgcaagc tgttgattca cattggatca caaaccgcca aatcgaagct gctcgtatcg
    35221 caatgactcg ttacatgaaa cgtggtggga aagtatggat taaaattttc cctcacaaat
    35281 cttatactgc caaagcaatt ggggtacgta tgggttctgg taaaggggca cctgaaggat
    35341 gggttgcacc agtaaaacgt ggtaaaatca tgtttgaaat cgcaggcgtt cctgaagaag
    35401 tagctcgtga agcgttacgt ctagcttctc acaaattacc aatgaaaact aagatcgtaa
    35461 aacgtgagga aatgggtggt gaatcgaatg aaggttaaag aaatcagaga attaaccact
    35521 gccgaaatgc ttgatcaaga aaaacaatta aaagaagaat tgtttaatct tagattccaa
    35581 ttagcaacag gtcaattaga aaacactgca cgtattaaag aagtacgtaa atcgattgca
    35641 cgcatcaaaa ctgtgttgcg tgaacaagct aagtaattat ggaaggaggc cattaagcgt
    35701 atgactgaag gaagaaatca acgcaaagtt tatcaaggtc gcgtggtttc agacaaaatg
    35761 gataaaacaa ttaccgttgc tgtcgaaaca aagaaaaacc accctatcta tggcaaacgc
    35821 atgaactatt ctaagaaata caaagtgcat gatgaaaaca acacagcaaa agttggcgac
    35881 atcgtgagaa ttatggaaac tcgtccatta tcagctacaa aacgtttccg tttgttggaa
    35941 gtagtcgaag aagcagttat tatctaataa cacgagattt tcctatatag attgaatgca
    36001 aaatctgaaa ggaggataca caacgtgatc caagcagaaa gtcgtttaaa agtcgctgat
    36061 aactcaggcg cacgtgaaat tttgacgatc aaagtccttg gtggatctgg tcgtaaaact
    36121 gctaacatcg gtgatgtgat cgttgctacg gttaaacaag caacgccagg tggggttgtc
    36181 aaaaaaggtg acgtcgttaa agccgttatc gttcgaacaa aatcaggagc tcgtcgtaca
    36241 gacggttcat atattaaatt tgatgaaaat gctgcagtga ttattcgtga tgataaaagc
    36301 cctcgtggaa cacgtatctt tgggccagtt gcacgtgaac tacgtgaaaa caacttcatg
    36361 aagatcgttt cactagcacc agaagtttta taatcttgag acacttatca aaggaggtgc
    36421 gaaacagtta tgtttgttaa aaaaggcgat aaagttaaag ttatcactgg aaaagacaaa
    36481 aacaaagaag gcgttgtact agcagcgttc ccgaaaaaag ataaagttat tgtcgaaggt
    36541 gtaaatgtcg ttaaaaaaca ccaaaaacct tctcaagctg ctcctcaagg tggaattgtt
    36601 gagatggaag cgccaattca tgtttctaac gtgatggtgg ttgactctac tggtgtagct
    36661 ggccgagttg gctacaaaga agtagacggc aaaaaagtcc gtgtttctaa aaaaaccggt
    36721 gaagttttag ataaataaaa gtttaaggaa ggagggtctt actgaatgaa ccgcctaaaa
    36781 gaaaaatatc ttaaagaagt aactccatca ttgatggaaa aatttaacta tagctctgtt
    36841 atgcaaacac ctaaagttga aaaaatcgtc atcaacatgg gtgttggtga tgcggtatca
    36901 aacgctaaaa acttagataa agcagttgaa gaattaactt tgatcactgg tcaaaaacca
    36961 atgattacaa aagctaaaaa atcaatcgct ggattccgtt tacgtgaagg aatgccaatt
    37021 ggtgcaaaag ttacattgcg cggagaaaga atgtacgaat tcttagacaa attggtttct
    37081 gtttcactac cacgtgtacg tgacttccat ggtgtaagta aaaaagcctt tgacggtcgt
    37141 ggaaactata ctttaggtat taaagaacaa ttgatcttcc cagaagtaga ttatgattta
    37201 gtagataaag tacgtggtat ggacatcgtt attgtaacaa cagcaaactc tgatgaagaa
    37261 tctcgtgagt tgttgacaca attaggcatg ccattccaaa aataattaaa ggaggcgaac
    37321 tacgtggcta aaaaatcaat gattgctaaa aacaaacgtc ctgcaaaatt ttcaacacaa
    37381 gcttatactc gttgcgaacg ttgcggacgt ccacattcag tttatcgtaa gttccatctt
    37441 tgccgtattt gcttccgcga acttgcctat aaaggtcaaa ttcccggcgt gaagaaagct
    37501 agctggtaaa aagtaaattc gcataagaag gaggtaaaag atcaatggtc atgacagatc
    37561 caattgcaga ttttctaact cgtatccgta acgcaaacat ggttaaacat gagtctttag
    37621 aagtacctgc atcaaaaatt aaacgtgaca tcgctgaaat cttgaaacgt gaaggtttcg
    37681 ttcgtgatgt tgaatacatc gaagatgaca aacaaggcgt gatccgtgtt ttcctaaaat
    37741 ttggtaaaaa cggcgaacgt gttatcacaa acttaaaacg tatctctaaa ccaggtttgc
    37801 gtgcttatgt caaagctgac gaagtaccaa aagtattgaa cggtttaggt atcgcaatca
    37861 tttcgacttc tgaaggtgtt gtcactgata aagaagctcg tgaaaaaaat atcggcggcg
    37921 aagttatcgc ttacgtatgg taatcaagta gaatctacaa ggaggtgtct ttaagtgagc
    37981 cgtatcggaa ataaagtcgt tgttatccct gaaggagtaa cagttactca agacggaaac
    38041 aacgttacag ttaaaggacc aaaaggtgaa ttgactcgta ctttttcagc agatatcaaa
    38101 atgaatatcg aaggaaacga agtaactttc actcgtccaa acgacagtaa agaaatgaaa
    38161 acaattcatg gaacaactcg tgcgaacttc aacaacatgg ttgtaggtgt cagcgaaggt
    38221 ttccaaaaag cgttagaact aatcggggtt gggtaccgtg cacaattaca aggatcaaaa
    38281 cttgtattga acgttggtta ctcacatcca gtagaaatca caccaccagc tggagtaact
    38341 gttgaagtac cagctaacac acaagtaatt gttaaaggtg cgaacaaaga agaagttggc
    38401 gaattagctg caaacattcg tggaactcgt cctccagaac cttataaagg taaaggtatt
    38461 cgttatgttg gcgaatatgt acgccgtaaa gaaggtaaaa ctggtaaata atcgtttaga
    38521 aaaattgcat agaagccagc agcaggaaaa aggcatttgg ctgaagttta ccttcaaaaa
    38581 aatacatttt tacttgtgcc gcagcttcta tttttcgaaa cgtatttact gcttgaaata
    38641 gtagtacaat aaaaataaag aggtgacaat tgtgattaca aaaccagata agaacaaaac
    38701 acgtcaaaag agacatcgtc gtgtacgtaa caatatctct ggtactgctg agcgcccacg
    38761 cttgaacgtt tttcgttcta acaaaaacat ctacgctcaa attattgatg acgtagcggg
    38821 tgtaacgcta gcaagtgcct ctaccttgga taaagaaatt tcaggtggaa caaaaacaga
    38881 agcagcacaa gctgtcggta aactagttgc tgaacgtgca gctgaaaaag gcattaaagt
    38941 agtagtcttt gaccgtggtg gataccttta ccatggccgt gtgcaagctt tagctgaagc
    39001 tgctcgcgaa aatggactag aattttagaa gaaggaggaa taccattcat ggtttatatc
    39061 gatccaaaac atttggaatt agaagaccgc gttgttgcaa ttaaccgtgt aacaaaagtt
    39121 gttaaaggtg gacgtcgtct acgttttgct gctttagttg ttgtcggtga caaaaacgga
    39181 cacgtaggat ttggtacagg taaagctcaa gaagttcctg aagcaatccg taaagcaatt
    39241 gaagatgcga agaaaaactt agttgaagta cctatggttg gttcaacaat cccacacgaa
    39301 gtaatcggtg cttttagtgg tggtagaatc ctaatgaaac ctgctgtaga aggttctggg
    39361 gttgctgctg gtggaccagt tcgtgccgta ttagaattag ctggggtagc agatatcact
    39421 tctaaatctt taggctcaaa tacaccaatc aacgttgttc gcgcaacagt tgaaggtttg
    39481 aaacaattga aacgtgcaga agaagttgca gaacttcgcg gcaaatcagt tgaagaatta
    39541 atcggataag gaggacaaca ataatggctg aattaaaagt tactttaaaa cgcagtatta
    39601 tcggacgtcc tcaaaaccaa cgcgatacag ttaaagcgtt aggtcttaca aagattaaca
    39661 gttcagtggt taagccttct aatgaagcaa ttaaaggcat gatcaatact gtttctcacc
    39721 tagtggacgt tgaagaaatt taatttagaa aaatcataac tagaacatta aggaggtgcc
    39781 aaacctatga aacttcatga attaaaacct gctgaaggtt cacgccaagt acgtaaccgt
    39841 gttggtcgtg gtacttcatc tggtaacgga aaaactgccg gacgcggaca aaaaggacaa
    39901 aaagctcgtt caggcggcgg tgtgcgttta ggatttgaag ggggacaaac accattgttc
    39961 cgtcgtttac caaaacgagg attcacaaac gtgaaccgta aagactatgc agtcattaat
    40021 cttgatgtat tgaaccgctt tgaagacgga gctgaagtaa ctcctgttgc tttagttgaa
    40081 gctggaatcg tgaaaaacga aaaagctgga atcaaagtat tagctaatgg ggaactaaac
    40141 aagaaattaa ctgtgaaagc agcaaaattc tctaaagcag ctcaagaagc aattgaagcg
    40201 gctggtggat caatcgaggt gatctaatgt ttagactatt aaaagatgct tttaaagtaa
    40261 aagacattag atcgaaaatc ctatttactg tatttgtgtt gtttgtattt cgcctaggtg
    40321 cacatattac tgtaccaggc gtaaatgctg cttcattgca aaacctttct tctttgccat
    40381 ttttcaacat gttgaacttg gtaagtggta gtgcgatgca aaacttttcc attttctcaa
    40441 tgggggtttc accatacatt actgcttcta tcgtcattca gcttcttcaa atggacatcg
    40501 tgcctcgatt tgttgaatgg tcgaaacaag gggaagttgg tcgtaaaaaa ctgaatcaag
    40561 cgactcgata tctgacatta gtattggcat ttatccaatc tgtcggtatc actgctggat
    40621 tcaatcaata cgctcaatta ggtttcgtga ataatcctaa cgtagcaact tacgtaacga
    40681 tcggtttgat tctaacagcc ggtacgatgt ttgttacttg gctaggagaa cagatcacag
    40741 aaaaaggtat cggaaatggc gtatcaatga tcatttttgc aggtatcatt tctagattgc
    40801 ctgaaagtat cagagaaatt tatgaagatt actttataaa tattgatcct tcagacatat
    40861 ggaaatctgc tatttttgtg gccattttac tagtagcggt cttgttgatc gtgacattcg
    40921 taacattttt ccaacaagct gaacgcaaga tcccgatcca atatacgaaa cgcgtagctg
    40981 gcgcgcctac aagcagctat ctgccgttga aagtaaatgc agctggagtt attccagtta
    41041 tctttgctag ttcgtttatt gctacgccaa atgctgtttt acaagcattg agcggtagat
    41101 tcggttctga aaactggtac aaagtgatga tggagatctt taactataat acagcacctg
    41161 gtgctacgat ttatacgatt ctgatcgttg ccttcacttt cttctacgcc tttgttcaag
    41221 taaaccctga gaaattagcg gaaaacttgc aaaagcaagg aagttatata ccaagcgtac
    41281 gccctggtaa ggggactgaa gagtatgtct caagattact gatgcgttta agtacagttg
    41341 gtgcagtctt ccttggttta gtggcattgc ttccaatcat tgctcaaatg gtttgggatc
    41401 ttccacaatc aatcggatta ggcggtacaa gcttactgat cgttatcggt gtagctttag
    41461 aaactgcgaa acagttagaa ggcttaatgc tgaaacgtaa atatgttgga ttcattgagt
    41521 aagaaagcgt gttgaggttt cccctcaaca cacgttctca cacatttatt aggagggaaa
    41581 acaatgaacc tcattttaat ggggctgcca ggcgcaggta aaggaacaca agctgaaaaa
    41641 atcattgatg cttatggtat tccgcacatc tcaacaggag atatgttccg cgcagcgatg
    41701 caaaacgaaa cagctcttgg cctggaagca aagtcttata tggataaagg tgcattagtt
    41761 cctgatgaag taacaaatgg aatcgtgaaa gagcgactag ctgaaccaga tacagagaaa
    41821 ggcttcttat tagatggctt tccacgtacg ctagatcaag caaaagctct ggacgcaatg
    41881 ctaaaagatt tgaataaaaa aattgatgct gtcatcgata tccatgttgg tgaagaaatc
    41941 ttggtcgaac gtctagccgg ccgatttatc tgcagcaatt gtggagcaac ttaccataaa
    42001 attttcaacc ctacaaaagt tgaagacaca tgtgatcgtt gcggtgggca tgaattctat
    42061 caaagagaag atgataaacc tgagacggtt aaaaatcgtc tggctatcaa catcaaaaat
    42121 agtgaaccaa tcttggctta ttataaagaa caaggtttgc taaacactat tgatggtgct
    42181 cgtgaaatcg atgctgtatt tgcagatgta caaaaaatca ttgaaaaata gcacgattgc
    42241 ccgtagccga cttgcattat gcagttactc ttgtcaagtt caataaatta tgatagaata
    42301 tgtcagtccg acttctagag aatcctctag atgatccatt ttgagaattt tgaggtggga
    42361 actgcagttt ccgccatttt atcgtgtgaa aatgcgaaac aagtacaagg aggtactgtg
    42421 cgtggcaaaa gaagatatga ttgaagtcga aggtacagtc gtcgaaactt tgccgaatgc
    42481 aatgtttaaa gttgaactag aaaacggaca ccaagttctt gctactgttt caggtaaaat
    42541 tcgtatgcat tacatccgta tcttacctgg tgacaaagta acggttgaat tgtctccata
    42601 cgatcttaac cgtggccgta ttacttatcg ctttaaataa ttgtactccg taaaactaca
    42661 ggaggtatta tcatgaaagt aagaccatca gtaaaaccaa tgtgcgaaca ttgtaaagta
    42721 attcgtcgta aaggacgcgt tatggtgatt tgcccagcaa atccaaaaca taaacaacgt
    42781 caaggataat tggaggtgct ataaatatgg ctcgtattgc aggagtagat attcctcgtg
    42841 ataaacgtgt agtagtttca cttacttata tctatggtat tggtaacact actgctaaaa
    42901 agatcttagc tgatgctggt gtatctgaag atgttcgcgt tcgtgaatta acgaatgaac
    42961 aaacagatgc tatccgtgca gaagtagata aattaaaagt tgaaggggat cttcgtcgtg
    43021 aagtgaactt gaacatcaaa cgtttgatgg aaatcggttc ttaccgtgga atccgtcatc
    43081 gccgtggatt gcctgttcgt ggacaaaaca cgaaaaacaa tgcacgtact cgtaaaggcc
    43141 cagctcgcgc tatcgctggc aagaaaaaat aattcttaag tgaaggaggt taaaacttca
    43201 tggcagcaaa aaaagtgaat cgtaaacgcc gtgtgaagaa gaatatagaa gcaggtattg
    43261 cacatatcca ttctacattc aataacacta tcatcatgat cactgatact cacggaaatg
    43321 cattagcgtg gtcatcagct ggttcattag gtttcaaagg aagcaaaaaa tcaactcctt
    43381 ttgccgctca aatggcagca gaaactgcta caaaagcagc aatggaacac ggattaaaga
    43441 ctgttgaagt aacagttaaa ggacctggtt ctggacgtga agcagcaatt cgttcattac
    43501 aagctacagg tttagaagtg actgcaattc gtgacgtgac tccagttcct cataatggtg
    43561 cccgccctcc aaaacgccgt cgtgtttaat gagtgccgct cattttgagt tttatcagta
    43621 acgtcattga acaagacact tttcgttttg aaaggggtaa agataagaat gattgagttc
    43681 gaaaaaccaa gaatcgcaaa aattgatgaa gaaaaagatt atggcaagtt catcgttgaa
    43741 cctcttgaaa gagggtatgg gactacttta gggaattccc tacgtcgtat tttattatct
    43801 tctttgccag gtgctgccat cactaatatt caaatcgatg gtgtactgca tgaattctct
    43861 accatcaaag gtgttagaga agacgtcact caaatcatct tgaacattaa aggcttagcg
    43921 ctgaaaatgt atggccaaga agaaaaaacc cttgaaatcg atattaccgg acctgctaca
    43981 gtaactgctg gcgatattat cgttgatagt gatgtagaaa ttcttaacaa agatatgtac
    44041 atctgtagtg tctctgaagg agctacgttc catgctcgct taacagtgaa acctggacgt
    44101 ggttatgttc aagcagatga aaataaaaaa gaagatatgc caattggcgt tcttccagtc
    44161 gattcaatct acacacctgt tcgtcgtgtg aactatcaag ttgagaacac acgtgttgga
    44221 caccgtgaag attttgataa attgacgatg gagatttgga cagatggttc aattgaaccg
    44281 ttggaagcaa tgagtttgtc tgcgaaaatc atgactgaac atttggatat ttttgtgaac
    44341 ttaactgatg aagcaaaaaa tgctgaaatc atggtcgaaa aagaagaaac acaaaaagaa
    44401 aaaatgttgg aaatgacgat cgaagagtta gacttgtctg ttcgctctta caattgtttg
    44461 aaacgtgcag gaatcaatac tgtacaagaa cttacaaata aatctgagcc tgaaatgatc
    44521 aaagtccgta acttaggacg taaatcatta gaagaagtta aagcaaaatt gcacgactta
    44581 ggtttaggtt tacgtaaaga agattaaaac cttttacgaa ggagggaaac caacgtgagt
    44641 tatcgtaaac taggacgcac atctagccaa cgtaaagcga tgttgcgtga tttaacaact
    44701 gatttattaa tcaacgaacg tattgtgact actgaagctc gtgcgaaaga agttcgctca
    44761 actgcagaaa aaatgatcac tttaggtaaa cgcggagatt tgcatgcacg tcgtcaagct
    44821 gcggctttcg tacgtaatga agttgccagc gtgcgtgaag aaaatgagga catcgtcatt
    44881 gaatcagctt tacaaaaact gttcaacgat attgcacctc gttacgcaga acgtcaaggc
    44941 ggatacacac gcattttgaa gacagaacca agacgcggtg atggtgcacc aatggttgtc
    45001 ttagaacttg tctaaaatca cactaataac atcactttat agatgattct tttagagtgt
    45061 tatgatgttg gaagatattt ttccgagtct agctcagcgc tgctcctcaa gcgaatatcg
    45121 cttggggagt aagcactcat cataaagtgt ttttttatac cttcaattac ctgtcatgac
    45181 agatatgttt gaaggcataa aaaaacattt taagcgagaa ataattgaaa attttgtctt
    45241 tttttgatag tgttagtagg aactttagga tgaagaagga gtaatcatgg agccaattat
    45301 tgaattagaa aagataaatt ataaatacca gccagatgat cttcgccccg cattgaaaga
    45361 tgtctccttt acaattgata aaggtgagtg gatcgctatt attggacaca atggttcagg
    45421 aaaatcgaca cttgccaaaa cgatcaatgg attgcttctg ccagaatcag ggatcgtgaa
    45481 agtgggaaat caaatattgg atgaagaaaa tatttggacg atccgacaga tggttggaat
    45541 ggtttttcaa aatccagaca atcagtttgt tggttcgaca gtagaagatg acgttgcttt
    45601 tggtcttgaa aatcaaggga ttcccagaga agaaatgcta gtgcgtgtga aagatgcact
    45661 tgagaaagtt agaatggctg agtttgcttc acgcgagcct gctcgtttat caggagggca
    45721 aaaacaacgt gttgccattg ccggcgtggt tgccttacga cctgatatca ttattttaga
    45781 tgaagcaacc agtatgctag atccagaagg tcgtgaagaa gtgatctcta cgatcaagaa
    45841 gatcaaagaa gaaagtcaat tgaccgttat atctatcact catgatattg atgaagcagc
    45901 taatgccaat cggatactag tgatgagaca aggcgaactt gtccgtgaag gtacaccaaa
    45961 agaaattttt tctgctggtc ctgaactgat cgacttaggt ctagatctac catttcctga
    46021 aaaactgaag agcgcactga aagaacgtgg ggttgatgtg cccagtgaat atatgacaga
    46081 agaaaggatg gtggattggt tatggacatc cgttttgaac aagtagactt cacttatcag
    46141 ccgaatacac catttgaaca aagagcctta tttgacatca atatgacgat caaagaaaac
    46201 agttatactg cattagtagg gcataccgga agcgggaaat caaccttact tcagcatttg
    46261 aatgcgcttg ttaagccaac aagcggtaca gtccatattg gagaacgaga tattcagccg
    46321 gatacggata ataagaattt aaagcccatc cgaaaaaaag ttggcatcgt ctttcagttt
    46381 ccagaagcac agctattcga agaaacggta gcaaaagaca ttgctttcgg tcctaaaaac
    46441 tttggtgtca gcgaagaaga agcactagtc ctagcaaaag aaacattaga acaagttggg
    46501 ctggatgaaa gctatttgga acgttcgcca tttgaacttt caggagggca aatgcgtaga
    46561 gtagcaatcg ctggtgtgct tgccatgagg ccggaagtac ttgtattgga tgaaccaacg
    46621 gcaggacttg atccccaggg cagaaaagaa atgatggaga tgttttggcg tcttcataag
    46681 gaacagcaga tcactattgt attagttacg catttaatgg atgatgtagc caattttgca
    46741 gattatgtct atgtgttgga aaagggacga atcgttaata gcggggagcc gcaagaggtc
    46801 tttcaaaaca ttgaatggct aaaagaaaaa caactaggcg tacctactgc caccgagttt
    46861 gcagaagaat taatggctaa agggatgaac tttgctacct tgcctttgac agctgaagaa
    46921 ttagcagacg ctatcgtcca ttatgctgga ggaaatgccc atgatgaata aattgatatt
    46981 tggtcgttat atgcctggag attccttgat ccatcgattg gatccccgag ctaaattatt
    47041 agccagtttt tattttatcg gaatcatttt tttagcaaat aattgggaaa cgtacctttt
    47101 gttggcattt ttcacattac tttctgtctt cttgtcaaaa gtagatttga aattttttat
    47161 ccgaggcatt cgcccattga tctggctaat cctcttcact gtagctttgc aggtcttgtt
    47221 tacaagtggc ggagaaactt attggagttg gggtgttttc catatcactg aatttggtgt
    47281 gatgaacggc ctctttattt tctgtcgatt cgtgttgatc atttttatgt cgactctact
    47341 cacgcttact acaccgcctt tggaattatc ggatgcgatt gagtacttgc ttcgtccatt
    47401 gaaagccgta cggttcccag tccacgaaat ttcattgatg ctgtcgattg ctcttcgttt
    47461 tgtgcctaca ttgatggacg aaacagaaaa gatcatgaac gctcagcgcg ctcgcggtgt
    47521 tgattttggt gaaggaaacc tagttcagaa aatgaaagca gtcattcctt tgctgattcc
    47581 tttatttgtc agcagcttca accgtgcgga agatcttgcg actgctatgg aagcaagagg
    47641 ctacaaaggt ggggaaggaa gaacgaaata tcgtgtattg cattggcata atagagacac
    47701 gatggttatc attgcttttg tattattaac tgtgctactg atctttttaa gaggataaac
    47761 gttcagtggg ccgctatcta ttcttttctt gctgatctgt ttgcaagaaa ggatgcaagc
    47821 agtgtatgac aagaaatatt agggtctgtg gcagactaag gtcacaggcc tctttctttt
    47881 atctgaaaag aaagaacgaa aaaattttat agaaaaatca ttattccaga taacctttta
    47941 ggaacaaagg agagaaaaac atggtacgtt acaaagcaat cattgcgtac gacggaacaa
    48001 attttaatgg attccaaaaa cagccaaatg ggcgtactgt tcaagaagaa gtagaaaaaa
    48061 cattacaaaa aatggcaaat ggaaaagaaa tcacggtctt tggttcggga aggacagatg
    48121 ccggagtaca tgcgatcggg caagtaatcc attttgatta tccagaggag cgtccgttag
    48181 aacgaatgcg tttcgcgcta gatacccagt caccagagga tattgctgta agacaagtag
    48241 aaattgtttc agaagacttc cacgcaagat atttggtcaa agaaaagacg tatcaatttc
    48301 gagtggatat agggaaacca cgcagtcctt ttcgacgcca ttatgcaagt tattttcctt
    48361 acccacttga tttggaaaaa attcagcggg cgcttcctga tttattaggg actcacgatt
    48421 tcacctcttt ttgtgcatca ggaagctcga tagaggataa aatacgtacg atctacgagg
    48481 ctaagatgga ggtaaatgaa acaaaagacg aattgttgtt tacatttagg ggaaatggct
    48541 tcttgtataa gatgatacgg atcttagtag gaacgctttt gaaaattggc aatggccgat
    48601 tgcctgaaga tagtatccca gatattattg cgaaaaaaga tcgtaatgca gcaggaccta
    48661 cagcacaccc tgaaggactt tatttgtatg aagtgaaata tgaataagag gttatgatag
    48721 aagtggtcag cttcaagaaa taagaaggta tttacgaaaa ttacttctca aaattttgca
    48781 aaatttcggc ttattttcga agaagctact tctggaacac cgtttaagga agaggtcgtg
    48841 aaatcagcgt tttgacctga gtattagaga aacaaacggg aaaatagctc ctcatatttt
    48901 gccgtttgtt gagctaatac cgaaggtcag ctgattgaac accgtttaag aaagaggtcg
    48961 tgagcctgtc atttaactct gagtcacaaa gagcaatttc aaaaaacagc ttctcatctt
    49021 tgatgaatat cgtgacttgt ggacgaggcg ttgataagtg aatcatcttt ttgttaccta
    49081 ttttttctgt aaccaaaaaa cgatggaacg ttgttttgat tgcccttggt gcggacagtc
    49141 tgaacaggtt ccatcgtttc tttattttct acgtcttctt ctgtgtgtgt ttcgtggttg
    49201 ttcttctatt gctggatcaa tcgtgccata aaaggggcga ttgcggtcaa ggtaccagac
    49261 ataaagaata aacaatgcaa tacataggac aaaaaggatt gcacctatcg taaatccttc
    49321 aggcaagtaa ctgaaagtga cagtatgttt acctgctgga acttttatca tcaagaatgc
    49381 gtctttgaaa ttgctgacct tgactggttt cccatcaatt ttagctttcc atcctttgtc
    49441 ataaggaata gttgtcacca acatggaatc ttcagcggta tttacagtcc cagaagcttt
    49501 tctgccagtg gcttccattt cgacaccttt ttctttgatc tgattcattg cggcttgata
    49561 ggctacaaca tctagaccaa cgactttagg ctccatgaaa cttatttctt tcgtcccata
    49621 aaaacttgca gtaaatgtca ctgttgtctc ttgaggataa tagccaagat catagtattg
    49681 accagaaatg ttgatttgcg tttttcgcga tgtaccattg acagtgatcg tcgcactaga
    49741 actttctaat tgcgcaaaat tggttgggaa caaacttaag taagcttgtg tatttgcagg
    49801 cacatggact tcccaagtga gctcttttgg ctggttatct gctgtttctg agtaagtgac
    49861 gccagtcgtt gtctttgtga tcgtcgtatt caactgattc ttcaaagtag gtgtatagaa
    49921 tgtaaaatac tgttcattta gaccagacaa tgtattgatc aagtttgtct gactggtcaa
    49981 attatctgtc actggctgct caacatcatt aacggacaac ggtgcagtaa atgctagtgg
    50041 caatgcattg acattctgat acagtgtata gtcgccgctt tgaccagctt ggcggaagcc
    50101 atatttactg aacgtgcttt cgtttttaga aatgttatat ttgattcctg taaacccatc
    50161 catcagcaaa gtattattgt tataacggat attcaaattt gtccctcttg aacggaaacc
    50221 taatttatcc aggtaagagg aagaattacg atttcttata gaagaaaaca aactgatacc
    50281 actgtaacca tagttgatcg aatcgtttgc agatactgga tccagatttt ccaagcggta
    50341 aaacgagtcg gaatcttttt ttgtttcatt tacaagagct ttcaagtcag gataaggatc
    50401 agtatataaa ctgcgggaag cataattcca atcatcaaga atgccatgga tcatactgtt
    50461 cgtattgacc agtgcttcag cagacatgat caataataac aatatactca gatgattgaa
    50521 tgaaagtttt ttatgctgat aaaatcccac agcaaaagca taaatcccta agaaaaccac
    50581 tgtcaataca aaggaagtca tcgtcacata ggtgtagctt ccttcaggtt tgatgcccca
    50641 tgccaccgca aagagagcag ctacgaccaa aatgattcct aacaacagcc cacgatcgtc
    50701 ttgttctatc ttttcccatc cttttcctgc aagcaggagc actaagaaag agaacagata
    50761 ggcataacgg aataaaaaca tattcggtgc atgcatacca tgccagaaca aattcaatgg
    50821 aacaagataa aaacttgcga taagtatggc gaaaagactg ccgtacaaaa atttattttt
    50881 caacggtact ttccgactgg caaaataaaa cagacagagg actaatggta gcagacctgc
    50941 gtaaacgaac ggaatcgatc cgtattttgt cgtgtcataa acaccaatca tatttttcat
    51001 gattagatcc agataagaag tcgcttctgt tttcaacgta gtgatctgac tcagtgcctc
    51061 gccatttgac cgaagatcca gcactgctgg caatacaatg atcattgaag cgccgcctgc
    51121 cagtaaagaa gtgataccat aaggaacaat cgttttttta tattctgacc agtttgtcaa
    51181 tagacgggcg ataaaatata agaaagaaaa tacgccaatc ataaaaccca tataaaaact
    51241 ggataaaaac aagagtaaat aactgataaa taaaagtttc ggccgtttaa gatccataac
    51301 acgatgaatc cccaaaatca ctaatggaag ataaaaaaat gcatccagcc acatgatgat
    51361 ctccgaatgt gcagtgataa atgagatcaa tgcgtaactg acgctaagtg caacatgccc
    51421 ccagcgagga atcttgaacg tacgtgcaag aaaccaaaaa gccaatccgg cacagccgac
    51481 cttaagcaaa gtcagaacat acagtgcatc tggcatcatc tggtttggaa aaaagaacac
    51541 cagtggcgtg aagatcccgc ctagatagta agaaattaaa gataaataat tcaatcctag
    51601 agaagcattc catgtataaa aaatactttg ctttccaaga agaacatttc ggaagctggc
    51661 atgaaaatta gaaaactgtg aaaaggcatc acttgccaaa atacttctgc tgcttccagg
    51721 atagattccg atacttagat aaacaagtgc taaaatcaaa aacggaatca ggaaactagc
    51781 tgctgcgtag cgtccgtttt ctctaaagaa tgactttaaa ttttttttca tatttatttt
    51841 ccaccaaatt tctatgaagt tgtattcagt ttatcatatc cttgtttttc tctgcttaaa
    51901 aagagtctag ttttcacaaa aaaataaaat ttagaataaa taacttcata atttgttcgt
    51961 ataacaaaaa taaacgcaaa aaaagggtag gaatcaaaag tcccaccctt aaaaattttt
    52021 attaaataaa aaaccgttca ctatctgaac ggtccaagta tcattcaatt aaacgcgttc
    52081 aacttttcct gatttcaaag cacgagttga cacccaaact tttttaggtt ttccgtcgac
    52141 cataacgcga actttttgta agttaggttt aacagtacgt tttgttgagt tcatagcgtg
    52201 tgaacggttg tttccacttt ttgtcttgcg acctgtaaag taacaaactt ttgccatggt
    52261 aatttcctcc tttgccttga tttggagcaa tagaataatt cagctcatac cagactaaca
    52321 tatcatatag aatgggaatt tgcaagtaca ttttcataga tacaattgac aattgagaag
    52381 aataattgta aattaaacaa ggttatacct gaaattttgc aaggtttttg agtactttgg
    52441 gtaactgcct tttatttcaa gttgcgctat gataaaatat gatagaagaa aaatgggagt
    52501 tttttacccg ttaaggaggc tcattcatgg ctgtaaaaat tcaaacgtca gctggaacca
    52561 ttgaaattac caatgatgta attgcaacag ttgttggtgg cgccgcgaca gatgtgttcg
    52621 gtgttgtagg aatggccagc aaaaatcaaa ttaaagataa tattactgag attttgcgta
    52681 aagaaaatta ctcaagagga gtagttgtcc gccaagaaga taatggtatt gctgtagatg
    52741 tttatacgat tatcagctat ggtacgaaaa tctctgaagt ttcccgcaac gtccaagaaa
    52801 aagtgaaata caatctagag gcattattag gcgtaactgc caattcagtt aatgtctttg
    52861 tacaaggtgt tcgtgtactg ccagactagg caggagactg gctggtaact cagcagtcga
    52921 tctgaaggag gattatctag gtgagtgtaa cagaaataag tgcaagccag ttccaggaaa
    52981 tggtccaggc aggtgcgaat cgtctgcaag taaatgcaga atacgtcaat tcattaaatg
    53041 tattccctgt gccagatggc gatacgggaa caaatatgaa tctatctatg actagtggag
    53101 ctaaggcagt agtagattca acctcagaaa aagtaggaga attagcagca atgctttcaa
    53161 aaggattgct gatgggtgcg cgcggaaatt ccggcgttat tttgtctcaa ttgtttcgtg
    53221 gattttctaa gcaaattcca gatgttgtct cattaaatgc ttctgatctt gctgcagcct
    53281 tcactcatgg agttgaaact gcgtataaag cagtaatgaa accagtagaa ggaacgattt
    53341 tgacagtagc tagagaagca gcgaaagctg gagaaaagaa agcaaaaagt actgatgatg
    53401 tcgttgaagt aatgacagct gttgtaaaag gagcaaagcg tgctttagct aaaactccag
    53461 acttgcttcc agtcctaaaa gaagtcggtg tcgtagacag tggagggcaa ggactgctgt
    53521 ttgtttatga gggcttttta agtgcattga atggagaatt ccaagcggat gaaacatatg
    53581 aaccttctcc agcagaaatg gatgatatgg tcaatgcaga acatcatcgc agtgtccaag
    53641 gtcaaatggc gacagaagat atccattttg gttactgtac agaaatcatg gtaaaaatcg
    53701 gagaaggacc tacagtcgat agtacattcg attacgaaga attccgtaac tatttagatg
    53761 gtataggaga ttctttactt gtggtcaatg atgacgagat catcaaagtc cacgtgcata
    53821 cagaaaaccc aggagaagtc ttgaattatg gacaaaaatt cggttcatta gtcaaaatca
    53881 aagtcgacaa catgcgcttg cagcatgaga cgattttgga acacgatgaa gaagtgaacg
    53941 agtttgcgaa aaaagaaata ccacgtatcc cgtatggtat cgtagcgatc gctgctggta
    54001 aaggcgtcca agagctgttt gaaagtttag gcgctaatta tgttatcagt ggtggacaaa
    54061 caatgaatcc aagtacggaa gacattgtta aagcgattca agaagtaaac gcagataaag
    54121 tgattgtctt gccaaacaat aagaacatct tcatggcagc tgatcaagca gcagaagtag
    54181 cagatatccc agtagcagtt gttccttcaa gaacgatttc ccaaggattg acagctatgc
    54241 tttcattcaa tgatcaagct tctttagaag aaaacaaaga aacgatgaca gaaatgttgt
    54301 ctagcgttgt gagcggtcaa gtcactcacg cgatcagaga tacctcaatt gacggtgtag
    54361 agatcaaaga aggagacttt ttgggcatga tcgatggaaa aatcctgatc tctaacccag
    54421 atatcacagc aacagcgtta agtacactag aacaaatgat caatgaggac acagagatcg
    54481 taacgatctt ggcaggtgat gaaggaacag ttgaacaagc aaatgaattt gctgagaaac
    54541 taacagaaaa acatcctgat ttggaaatcg agatccacca aggagatcag cctgtctatc
    54601 cttatctatt ttctgctgaa taatttctgt gtcagaatat ctttctaaca cagtattcat
    54661 tcataaaagg ccttcggtta gtgatcaggt atgatcgctg accaagtcct ttttttgtta
    54721 aaggagggag gaaaaattta tgcgttcttt agatgatcca atcactatgc tggcaggagt
    54781 cggagaaaaa agagcagcta gtcttgccag cttgggcatc caaacgattg aagacttact
    54841 gacttattat ccttttcgct acgaagatat tcaggaaaga aatctaaacg agatccagga
    54901 tcaagaaaag gttactttga aaggaatcgt agtctcgcct cctgtgatga atcgatttgg
    54961 ttataaaaaa agccgattgc aatttcgtat gatgcaagaa catgatgtgt tcaacgtttc
    55021 tttttttaat caaccttatc taaaagaaaa agtcatttta tcggaggaaa tcgccgtata
    55081 cggtaaatgg gatgcaaagc gtaaagcctt gaacgggatg aagattttag gttctcaaac
    55141 aatggatgac ttttcgccga tatatcacgt caacaaatca attcgtcaaa cgacattagt
    55201 tgatctaatc aaaaaagggt ttcaagaata cggcacattg attgaagaaa atctgccaaa
    55261 caaccttgtg gagaaatatc gactgcttga ccgagcttct gctgtaagaa gtatgcattt
    55321 tccaaaagac cacgaagaaa accatcaagc caaacgacgt gtcgtttttg aagagttctt
    55381 tttatttcaa atgcgtcttc aaggattgaa aaaagcagaa aaagcagaaa cgaatggttt
    55441 ggagatcctt tatgatgtcc aaaaattgaa acaatttact caaaagctac cttttgaact
    55501 gacaaatgca caaaaacgtg taacgaatga aatctgtcgg gatcttcgca gtccgcagca
    55561 tatgcagcga cttttgcagg gagatgtagg tagcggaaaa acagtggttg ccgcgattgc
    55621 gttatacgct accgttacag caggtttcca aggtgcgttg atggtgccta cagaaatctt
    55681 ggcacaacaa catatggaaa gcttgcaaca gctgtttgat cccaatgaag taaccatcgc
    55741 attgattacg gggtcaacga aagcaaaaga acgacgtgag ctccttgaaa aactagagca
    55801 aggggaaatt aacgtcgtga tcggcacaca tgcattgatc caagatggag tcgaatttca
    55861 gaatttaggt ctagttatta cggatgagca gcaccgtttt ggcgtgaacc agcggaaagt
    55921 acttcgtgaa aaaggctggc gaccagatgt cttatttatg acagcgactc cgattcctag
    55981 aacattagct attacagcat atggtgagat ggatgtatct atcattgatg aacttccagc
    56041 tggacgtatc ccgatcgaaa cacgctgggt aagaacacct caattggaca gtgtactgga
    56101 ttggacgaaa aaagaactgg caagaggaca tcaaatgtat gtcatctgtc cgctgattga
    56161 agaatcagaa gcattagatg taaaaaatgc agtggaaatc tatgaaaaac ttcgtgaact
    56221 atttgcacca aaatatgaag ttggcttgtt acatggtaaa atgaagaatc aggaaaaaga
    56281 agtaatcatg gaagccttca aagacaacca gatgcagata ttagtttcga ctactgtaat
    56341 tgaagtaggg gtaaacgtgc cgaacgcgac cgtcatgctg atcatggatg cggatcgatt
    56401 tggattggca cagctgcacc aacttcgtgg acgtgttgga cgcggaagtg atgcttccta
    56461 ctgtatctta gttgctaatc ctaaaaatga actaggtgtg gaacggatga aaatcatgac
    56521 agagaccacc aatggttttg tactaagcga aaaagatctg gaattacgtg gtcctggtga
    56581 agtatttggc ttccgtcaat ctggtctgcc tcaatttgcg attgctgact tggtgacgga
    56641 tggaaatgtt ttggaggtag ccagagaaga ggctgcagcc ctttggcaaa taaaggattg
    56701 gcagatgctt cctgagtatc agcctttagc gcaaagcctc caaatagaaa atcaggataa
    56761 tcgttatttc gattagcaga ctgtgctata ctttatactg atgacggaaa aaggagtgga
    56821 aacaatgaaa attgccgtag atgcaatggg cggagatcac gcacctcaag caattgtaga
    56881 aggcgtggct ttagcgcagc aagattttcc agaagtagaa ttcttacttt acggaaaaga
    56941 agcagaaatc aagaaatact taacaaatga aaaaaacatc acgattatcc atacagacga
    57001 aaaaatcaat agtgatgatg agccagtaaa agcaattcga cgaaaaaaga ctgcatcaat
    57061 ggtccttgct gcacaagcag tgaaaaacgg tgaagcagat gctgtatttt cagcagggaa
    57121 tacaggcgcg ctactagcag cagggttgtt catcgtagga cgtatcaaaa atatcgaacg
    57181 ccctggcttg atgtctacct tgccagtagt agggaaagaa ggtgccggtt tcgatatgct
    57241 ggatcttggc gctaacgcag aaaacaaacc agaacatttg ttgcaatacg gtattttagg
    57301 ttctttttat gcacgtaaag tccgaggtat cggacgtcct cgtgtcgcac tcttaaacaa
    57361 cggaacagaa gcaacaaaag gtagtgaagt gacgaaaaaa gcctatgaac tccttcagaa
    57421 tgaaacaagc ttgaatttta ttggaaacgt agaagctcgt gagctactca atggtgtggc
    57481 tgatgtagtg gtcacagatg gatttacagg caacgctgtt ttgaaatcta ttgaaggaac
    57541 agcaatgaat atgatgaatc tattaaaatc tgctattcta aatgaaggga tcaaaggaaa
    57601 aatgggcgct atgcttttaa aagacggctt acgttctttg aaagcagaaa tggattattc
    57661 caaacatggc ggtgccgttt tgtttggctt gaaagcacca gtcattaaaa ctcatggagc
    57721 aacaggcccc gatgctgttc gctatacgat ccgccaaatc cacacaatgc ttgagacaga
    57781 tgtagttgga caattagtcg aacaatttga aaaaagcgaa gaatagtatt tggatatctg
    57841 aagcgatccg ttccattgaa aaaaaggtgg acattcctga aaaatgccag taaaattagc
    57901 catggtgtag gtaattgaca agtgtatgat tcttggaggt gaaaaagttg actcgcgaag
    57961 aagtatttaa caaagtagcg aaaattattt caaatcattt tgagatagat accgataaag
    58021 tgacggatga attgaacatc aaagatgatt taaaagctga ttcaattagt attatggaat
    58081 tcgttttaga gcttgaagat gaattcggta cggaaatttc agatgaagac gctgaacaaa
    58141 ttgaaacagt cggtggcgca gtagattata tcagcagcca tcttaaataa tttcaaataa
    58201 aagcaaagaa ggataaaaag ctgcatgcag ctttttatcc ttcttttttt aatctttttg
    58261 acacgtaaaa gtcatttttc tgataattct ggc
//
LOCUS       DACCXO010000006        57806 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.006, whole genome shotgun sequence.
ACCESSION   DACCXO010000006 DACCXO010000000
VERSION     DACCXO010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.006"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            96..1613
                     /locus_tag="IVY70_000318"
     CDS             96..1613
                     /locus_tag="IVY70_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP7957481.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGSWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            1603..2874
                     /locus_tag="IVY70_000319"
     CDS             1603..2874
                     /locus_tag="IVY70_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7957482.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            3049..3945
                     /locus_tag="IVY70_000320"
     CDS             3049..3945
                     /locus_tag="IVY70_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7957483.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVEAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            3959..4822
                     /locus_tag="IVY70_000321"
     CDS             3959..4822
                     /locus_tag="IVY70_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7957484.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            4828..5391
                     /locus_tag="IVY70_000322"
     CDS             4828..5391
                     /locus_tag="IVY70_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP7957485.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            5401..6924
                     /locus_tag="IVY70_000323"
     CDS             5401..6924
                     /locus_tag="IVY70_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP7957486.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            6936..7895
                     /locus_tag="IVY70_000324"
     CDS             6936..7895
                     /locus_tag="IVY70_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP7957487.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            7981..9060
                     /locus_tag="IVY70_000325"
     CDS             7981..9060
                     /locus_tag="IVY70_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7957488.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(9312..10712)
                     /locus_tag="IVY70_000326"
     CDS             complement(9312..10712)
                     /locus_tag="IVY70_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP7957489.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            10930..12591
                     /locus_tag="IVY70_000327"
     CDS             10930..12591
                     /locus_tag="IVY70_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP7957490.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            12548..13246
                     /locus_tag="IVY70_000328"
     CDS             12548..13246
                     /locus_tag="IVY70_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP7957491.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            13262..14686
                     /gene="araA"
                     /locus_tag="IVY70_000329"
     CDS             13262..14686
                     /gene="araA"
                     /locus_tag="IVY70_000329"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP7957492.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            14993..16153
                     /locus_tag="IVY70_000330"
     CDS             14993..16153
                     /locus_tag="IVY70_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP7957493.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            16271..18130
                     /locus_tag="IVY70_000331"
     CDS             16271..18130
                     /locus_tag="IVY70_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP7957494.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            18240..18674
                     /locus_tag="IVY70_000332"
     CDS             18240..18674
                     /locus_tag="IVY70_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957495.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(19113..20135)
                     /locus_tag="IVY70_000333"
     CDS             complement(19113..20135)
                     /locus_tag="IVY70_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP7957496.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            20263..20616
                     /locus_tag="IVY70_000334"
     CDS             20263..20616
                     /locus_tag="IVY70_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP7957497.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20628..21794
                     /locus_tag="IVY70_000335"
     CDS             20628..21794
                     /locus_tag="IVY70_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7957498.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(21886..22506)
                     /locus_tag="IVY70_000336"
     CDS             complement(21886..22506)
                     /locus_tag="IVY70_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP7957499.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(22698..22994)
                     /locus_tag="IVY70_000337"
     CDS             complement(22698..22994)
                     /locus_tag="IVY70_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP7957500.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            23198..23848
                     /locus_tag="IVY70_000338"
     CDS             23198..23848
                     /locus_tag="IVY70_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7957501.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            23864..25591
                     /locus_tag="IVY70_000339"
     CDS             23864..25591
                     /locus_tag="IVY70_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7957502.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            25588..27480
                     /locus_tag="IVY70_000340"
     CDS             25588..27480
                     /locus_tag="IVY70_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7957503.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            28184..29053
                     /locus_tag="IVY70_000341"
     CDS             28184..29053
                     /locus_tag="IVY70_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP7957504.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            29262..30287
                     /locus_tag="IVY70_000342"
     CDS             29262..30287
                     /locus_tag="IVY70_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP7957505.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            30326..31276
                     /gene="manA"
                     /locus_tag="IVY70_000343"
     CDS             30326..31276
                     /gene="manA"
                     /locus_tag="IVY70_000343"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP7957506.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            31370..33073
                     /locus_tag="IVY70_000344"
     CDS             31370..33073
                     /locus_tag="IVY70_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7957507.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            33075..35108
                     /locus_tag="IVY70_000345"
     CDS             33075..35108
                     /locus_tag="IVY70_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7957508.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            35119..35559
                     /locus_tag="IVY70_000346"
     CDS             35119..35559
                     /locus_tag="IVY70_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7957509.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(35759..36886)
                     /locus_tag="IVY70_000347"
     CDS             complement(35759..36886)
                     /locus_tag="IVY70_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7957510.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(36889..37566)
                     /locus_tag="IVY70_000348"
     CDS             complement(36889..37566)
                     /locus_tag="IVY70_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7957511.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            37830..39596
                     /locus_tag="IVY70_000349"
     CDS             37830..39596
                     /locus_tag="IVY70_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP7957512.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            39611..40048
                     /locus_tag="IVY70_000350"
     CDS             39611..40048
                     /locus_tag="IVY70_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7957513.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            40061..41212
                     /locus_tag="IVY70_000351"
     CDS             40061..41212
                     /locus_tag="IVY70_000351"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP7957514.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(41274..41972)
                     /locus_tag="IVY70_000352"
     CDS             complement(41274..41972)
                     /locus_tag="IVY70_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP7957515.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(41965..43317)
                     /locus_tag="IVY70_000353"
     CDS             complement(41965..43317)
                     /locus_tag="IVY70_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP7957516.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(43540..44940)
                     /locus_tag="IVY70_000354"
     CDS             complement(43540..44940)
                     /locus_tag="IVY70_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP7957517.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            45101..45838
                     /locus_tag="IVY70_000355"
     CDS             45101..45838
                     /locus_tag="IVY70_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP7957518.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            45963..46442
                     /locus_tag="IVY70_000356"
     CDS             45963..46442
                     /locus_tag="IVY70_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP7957519.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            46439..47206
                     /locus_tag="IVY70_000357"
     CDS             46439..47206
                     /locus_tag="IVY70_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957520.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            47436..48005
                     /gene="lepB"
                     /locus_tag="IVY70_000358"
     CDS             47436..48005
                     /gene="lepB"
                     /locus_tag="IVY70_000358"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7957521.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(48109..48711)
                     /locus_tag="IVY70_000359"
     CDS             complement(48109..48711)
                     /locus_tag="IVY70_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7957522.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            48817..49286
                     /locus_tag="IVY70_000360"
                     /pseudo
     CDS             48817..49286
                     /locus_tag="IVY70_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            49392..49976
                     /locus_tag="IVY70_000361"
     CDS             49392..49976
                     /locus_tag="IVY70_000361"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP7957523.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            49979..51052
                     /gene="prfA"
                     /locus_tag="IVY70_000362"
     CDS             49979..51052
                     /gene="prfA"
                     /locus_tag="IVY70_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP7957524.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            51045..51884
                     /gene="prmC"
                     /locus_tag="IVY70_000363"
     CDS             51045..51884
                     /gene="prmC"
                     /locus_tag="IVY70_000363"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP7957525.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            51877..52056
                     /locus_tag="IVY70_000364"
     CDS             51877..52056
                     /locus_tag="IVY70_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957526.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            52078..53103
                     /locus_tag="IVY70_000365"
     CDS             52078..53103
                     /locus_tag="IVY70_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP7957527.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            53278..54522
                     /locus_tag="IVY70_000366"
     CDS             53278..54522
                     /locus_tag="IVY70_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP7957528.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            54675..55622
                     /locus_tag="IVY70_000367"
     CDS             54675..55622
                     /locus_tag="IVY70_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7957529.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            55756..56385
                     /gene="upp"
                     /locus_tag="IVY70_000368"
     CDS             55756..56385
                     /gene="upp"
                     /locus_tag="IVY70_000368"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP7957530.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(56524..56766)
                     /locus_tag="IVY70_000369"
     CDS             complement(56524..56766)
                     /locus_tag="IVY70_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957531.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            56887..56959
                     /locus_tag="IVY70_000370"
     tRNA            56887..56959
                     /locus_tag="IVY70_000370"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56920..56922,aa:Thr,seq:cgt)
     gene            complement(57078..57716)
                     /locus_tag="IVY70_000371"
     CDS             complement(57078..57716)
                     /locus_tag="IVY70_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP7957532.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT        19213 a        10112 c        11955 g        16526 t
ORIGIN      
        1 aaaatcgata aaaaataagt aagtttaaca tttatattat tgacaacgct ttcaacgagg
       61 tgtattattc atgtataaat taaaggaggt cagttatgga agccacaatg ttgattagta
      121 gtgtcaagcg tgtaggcaaa gttgatccgc ggttgtacag ttcatttatt gaacatatgg
      181 gacgagcagt atatgaaggg atttatcagc cagatcatcc atcagctaat aaaagtggat
      241 ttcgccaaga cgtcattcac ttggtaaaag aattgaacat tcccatgatt agatatcctg
      301 gtggaaattt tgtgtcagga tttcgttggg aagacagtat tgggcccata gcaaatagac
      361 ctagaaggtt ggatttagct tggcggacaa ttgaaacgaa tgaagttggg attcatgaat
      421 tttataactg gtgtcagcat gtgggggccg aaatcaatat ggctgtcaat ctgggtactc
      481 gtggagtaga tgcagctcgt aatttagtcg aatattgtaa ttttcctaaa ggaacttatt
      541 ggagtgattt aaggcgttcg aatggtcaag agagcccttt tggaattaag acatggtgtt
      601 tagggaatga aatggacggt tcttggcaga ttggacataa aacagcggaa gaatatggac
      661 ggttagcaga ggaaacggct aaggcgatga aactcgtaga tgattcaatt gaggtggttt
      721 tatgcggaag ttcgaatcgg caaatgccta catttggtga ttgggagtta acagttcttg
      781 atcaagcgta cgatcaaata gattatttgt ctctgcatca atattatggc aaccaaaaaa
      841 atgatctttc tcattatctt gcacgttcct tagacatgga tcggttcatt tctgaagtga
      901 cagcgatgtg cgatacagtc aaggcgaaga aacatagtaa gaagcagatc aacctgtcct
      961 ttgatgaatg gaatatttgg tatcattcag cggaacaaga tgagcaagca gcgccttggc
     1021 agattgctcc tcctttacta gaagataact ataattttga agatgcatta ctattaggct
     1081 gtctattaat cactttattg aaacatgcag atagagtaaa gattgcttgt cttgctcaat
     1141 tggtaaatgt tattgcacca atcaggacag aaacaaacgg agaagcttgg agacagacga
     1201 tctattatcc atttatgcaa gctgctgtgt atggacaagg agaggtgtta agcccacaaa
     1261 ttcgttcagc aaactatgcg acggaagatt ttgaagaagt gccgtattta gaaagtatag
     1321 ccgtaataaa tgacaaagaa cttgtcatat ttgcagttaa ccgtggagaa gaagaaatga
     1381 atctaactgt tcacttgtct gaattatcat tagagggagt gattgatttt tctgaaatga
     1441 gtggatttga tacaaaagca actaatcttc ctaaaagtga acaagtaaaa ccaagtcatt
     1501 caacaaatgt aaaagctgaa gatgggaaaa tcaattgttt attgaaaccg ctatcttgga
     1561 atgtggtcag atgtcgttta tcagatgaag gaggaacaaa aaatgaaagg taaaaaatgg
     1621 ttaattggct ctttgttggt agctgcaaca ggtattttag gagcttgcgg tggcggaagt
     1681 tcagcagaat ctgattctga taaagaagtg actttgactt tttggaatgg gtttactgca
     1741 tctgatggtg aaatattgca agaaatcgtt aatgaattta ataaaacaaa tgataaaaac
     1801 atcaaaattg aaatggatat catgacttgg gccaatttga atgaaaaact tcctacagcg
     1861 atttcttcta aaaaagcacc tgactttatt gcattaaatt atccagattt tgcacagtat
     1921 gtagaaaacg gagcggtaca accattagac gatttctggg aatatgatgg agtagataaa
     1981 tctgatttta aagaaacggc tattgaatta ggtgaagtag aaggtaaaca gtattttgtc
     2041 cccatgcagg tacaaggcat gtatatgtat tggaataatg atttgtttaa acaagctgga
     2101 ttagatactg aacaaccacc tactacttgg gaagaattga cagaaatggc accaaaatta
     2161 actgatagta gtaaaaatgt atcaggtttt gtcttcaata aggatggaac tgcaccgtta
     2221 tataactgga tgttagcaaa tggcggtaaa ttagtgaacg atgattatac taagtctgaa
     2281 tttgcgtctc ctgagaacct taaaacgtta aaagcgatcc aaaaaatgat ccatgtagac
     2341 aagactggtc cagaatcgat ttctggcgcc gaaatggaca atttaatgaa tgctggtcaa
     2401 ctagccattg aaatcaatgg gccatggtta aataacggat tgaaagcaaa cgagattgat
     2461 tataatgtga ctaccttacc acaagggagt gattcagaca aaaaaacagc tattttagat
     2521 ggcgtaggat atgccattcc ggcaagtaca gataataata aaaaagaagc tgtctatgag
     2581 tttttgaaat attggaatac aactgaaatt ggtaaaaaat ggagtatcga aaatggtttt
     2641 cctgcctact tggattcagt agcaaaagat gaagaagtaa aaaatgatcc tattgtatct
     2701 gaattatcga aacaaatgga ctatgcagag ccatatttac caggttttac ccaaatctct
     2761 gcgatcaata acgatatcat caatccactg attgaaaaat tattagcagg agaagatcct
     2821 gagacactca tgaacgatgc agatgatgcc atcaatcaat tactgcaaaa atagtacaga
     2881 actttataca ggatagactg ttggcaagat acagatatct gaatgatcga cctagtattt
     2941 gcagataggt gttattgcaa aaatcacagc gattgttcgg atatcttatt cagattcgtt
     3001 taaaccaaga ttgtatattt tatcaaaatg aaagatgagg tggactatat gaacggattc
     3061 accaaatatt ggaatcgtcc tgatcgtgct gcgtatttgt ttttaactcc atctttttta
     3121 gttttattat tatttactgt cataccgctt ttaggaacat ttgctattag ttttacagat
     3181 ttgaatattt tctttacatc aagaaatttt gtaagttttg aaaatttcac acgtatattt
     3241 agtgatgaac gagcagttaa ttcgttgatt cattctttat atttcaccgt actcgaaacg
     3301 ccaacacaaa ttatcgttgg tttattagca gcagtagcat tatctaaaaa taccagattc
     3361 aatcgttttt gtcgttcgac attttacatt ccagttattt gttcattgac gtcaattgcg
     3421 attatttttt ctatgttgct tgatccgaat gtagaggcga ttccttactt gttttcacaa
     3481 gtcggcttgc cgatccctca attttttcgc gatccaaccc ttgctatgcc gacggtagcc
     3541 atcatgactg tttggaagaa ttttggtgtt actatgacga ttctcttgac tgctattcag
     3601 ggaatctctc cctctttgta tgaatctgct caaatggatg gtgcaacaaa tcgacagcag
     3661 ttttttaaca ttacgctacc tcagatcgtt ccctccttag gtttttgtat tttaacgaat
     3721 ctgattggtt cgatgcaagt gtttgaccaa gtatatgttg caaccgctgg tggcccacaa
     3781 tttaagacag aaacagctgt tcaatatatt tacagtcgag gatttacagc accttatgaa
     3841 ttgggctatg catcagcact gtcttcggtt ttgtttatca ttgttgcaac tatagctatc
     3901 tccactaatg tgtacatgtc acggaaagaa aaacaaatga aataggaggg aaacctgtat
     3961 ggcaccagaa agctcgatta cagttactgt gaatagtcga aaagcagtta gtggtaaaaa
     4021 aatcattcga acgattagta tgctagttat attgttggtt attgcgtttc cttttttgtg
     4081 gctgattatt tcttctttta aacacgaaaa agacattatc tcatttccgc ctagaatatt
     4141 tgcagatagc tataccctag ataactatat taaggtttgg acaacgatcc ctttattaga
     4201 ttacatcaaa aatacagtga tttttgctgg agggacagtg atcacttctg tattctttga
     4261 ttctcttgcg gggtatgcat ttgctcgtat gcgtttcaaa ggaaaatcag ttttgttcta
     4321 tttcgttttg ctgacaatga tgatcccatt ccaagtcttc atgattcctc tatttattga
     4381 agtcaatttg cttggcatgc ttgatactta tgcaggtttg atcattcctc gtatgacaac
     4441 tgcatttggt atcttcatga tgcgttcctt ttttattact ttaccagatt ctttggaaga
     4501 ggcagcacgc attgatggat taagtgagtt taatattttt ttgaaaatca tgttgccatt
     4561 gagcaaacct actcttttat cattagggat tttcacgctg atgaatagtt ggaatgactt
     4621 gctttatcca ttgatattga caagtagttc aaaaatgaga actttaccag cagggttagc
     4681 tttatttaca gggcagaaca tttcttttta tggacctgta atggctggaa cggttatttc
     4741 aatgctgcca ttactagtgg tttatatttt tgcacagaaa tattttgttc aaggaacagc
     4801 tatgtctgga atgaaagagt gaggaagatg aaaatagttg agactaaact ttatggacag
     4861 ttgattgtgg tttctgatta catcttatta ggtattctct gggtaatcgt gtgtttgcct
     4921 gttattacta tcgtgcctgc tacatgtgct gttttttatg taatggcgca atggaaaggt
     4981 gagggatcag gaaatatcat ttattttttc tttcaaggaa taaagcaaca ttttggtaag
     5041 aacttgttcc ttagtctgtt aacgatcatt atttattttc ttgttaatca attattagaa
     5101 gaacagacaa tcgtactttc aattagtgga tatgtagttt tacttattta ttccatgttt
     5161 cttttatcct ggatatatgt gatttctgaa aacaaagata tcacattttt acaaattttt
     5221 gaacaaagtg catggaaagt attttttgat tttttgagaa atattctttg ttgcgtattg
     5281 ttattaggat ttgtactgct catatttctt ttccctcctt ttatttttat ctttgctggt
     5341 ggcgtatgga aacttgtatc tattatcttg actataggaa atgaaaggta gggaaagctg
     5401 ttggacgaag aagtgaaagt aaaaatcaga gaggatttta cggtaggaga gatcagttct
     5461 cggctgtttg gctcatttgt tgagcatatg ggaagtgtcg tctatacagg gatttatgaa
     5521 ccgggccatg aaacagctga tggtgacggg tttagacaag atgtcctctc ttatgtaaag
     5581 gaattagaat taggtgtcat tcgctatcct ggaggaaact ttacatctgg ttatgattgg
     5641 gtggatactg taggtaggaa agaacaacga ccaaaaaaaa ttgatttagc atggcgaggt
     5701 atagaaccaa attcttttgg actccacgaa ttttttaaat ggatcagtca tgtacaagcg
     5761 aaaccgatat tcacggtgaa tttaggaaca aaaggaattg atgaagcaaa aaatttagta
     5821 gaatattgta attttccaaa aggaaccttc tggagtgatt taagaataaa aaatgggcaa
     5881 aaaaagcctt ttggcgttaa attatggtgt ttagggaatg agctagacgg tccatggcaa
     5941 gtgggggcta agagtgctta tgaatatggt aggttagcaa atgaagcctc aaaagtcatg
     6001 aagttagtgg atgattcaat tgaaacaatc ttagttggaa gctctacgcc acgtttacca
     6061 tcgtaccctg aatgggatcg tatcgcatta gaagaagctt atgagaatgt agattattta
     6121 gctttgcata actatattga taagtatgat gaagaagact tgactaaacc tcctaaaaat
     6181 gagcgtgatg atacagcgac ataccttgca aaagcttatc gattcgatcg tcaaattgaa
     6241 gaaatcgttg ctacttgtga ttatgtaaaa ggatatttga gaagtgagaa aacaatgtat
     6301 ctagcgtttg atgaatggaa tgtacatgcc caaccggaaa aaagacatag ggattttgaa
     6361 gtcggtagtc ccatcgattg gtgttacttt actatggaag atacattatt atttggttca
     6421 ttagggctgg ctattttacg acacgcagat cgcgtaaaga ttagttgtca atcattgtta
     6481 gtaaacacaa tacctttaat actgacagat gctaaaggac aagcatggag gaatccaact
     6541 tattatgtga tgcaacactt atcgaaatat gctaaaggtc aagtattgaa acaacaaatc
     6601 atttgccctg tttatgattg tgaaaagata aaaaatgttc cttatgtgga cagtgtgatc
     6661 gtagataacg gcaatcagct gactattttc ttgatcaatc gcacaaatga acaacaaaag
     6721 ataacgattg aacatgattt caatttgcta gatcaaggag agcactacac attaaccagt
     6781 agcgatctgt tggacaaaaa tacacgtgaa gaaccagaaa aaataaaacc tgaaataaac
     6841 tatttttcaa tagaaaaaca tgacgaatta aatgtaggga tagaaaaata ctcttggaat
     6901 gtaattagaa taaagaaaat gtgaggaaat ttgttatgat gaagacactt tataataatc
     6961 ctatcgttcg gcaacgggca gatccttggg tatataaaca tacagatggt tattattatt
     7021 ttacaggatc ggttccaggc taccaagtga ttgaattaag aagggcaaag tcactgaatg
     7081 aattagaaca tgctgcaagc ctgattgtat ggcaggctca tgaagaagga ccgatgagtg
     7141 aattgatctg ggcacctgaa gtccattata tcaatggaaa atggtatatt tactttgctg
     7201 caagcaatca taaaactata cgtgatgaat ctcatcatca ccgcatgttc gtacttgaaa
     7261 atcagcatat agatccaatg aatacagatt ggatagaaaa gggacaaata aaaacgaaaa
     7321 gggaaacttt cagtttagac ggaacaactt tctcttctca aggaaagcta tattatgtgt
     7381 gggcccaaca agatcctgaa attactggga attctaatct ttatctatcc gaaatggata
     7441 atccttggac gttaaagggg aagcagatcc tcctttctat acctgagtat gaatgggaaa
     7501 aacggggttt ttcagtaaat gaaggtccag cagtccttac tcgcaatggg aaaatattca
     7561 ttacgtattc tggtagtgca acagatgaaa actatgccat gggcctgctg tgggctgatg
     7621 agcacaagaa tcttttatta aaagaatcat ggcataaatt atcggagcca gtattcgttt
     7681 cttctgaaaa aaataagcaa tttggtcctg gtcacaactc gtttacggtt tcagaagacg
     7741 ggtgctttga tattttgatc tatcatgctc gaccagagaa gaaccaaaaa ggggatccgt
     7801 tagacaatcc taatcggcac gcgaacgcgc aatgctttac gtggaatgag caaggatttc
     7861 cagaatttgg tgaacctgtt ccgtatgcct tatgagcaca agaaaccttg atttttgctt
     7921 gaataaacct tgcagactcg ttatgatata tgtacgtaca aataaaaagg ggcaaggcaa
     7981 atggcaaaat atcaagatat tgcagaagag ataaaacaaa agatccttcg tgaggaatac
     8041 gaagtaaata caaccattcc ctcagaattc aagctgcaag aaatatatga cgtcagcaga
     8101 catacgattc ggcaagcaat cgctgtgttg gtaaatgagg gctatctgcg taaagaaaaa
     8161 gggtcgggca catatgtaga tgacagctat aagaaagaaa aaacaccatt gaacagaaca
     8221 aaaacgatcg gtgtcatcac cacatatctc tctgattata tttttccgtc gattatccga
     8281 ggaatcgaaa atgaattacg agaatctggg tattcattgc ttttagccag tacgaataac
     8341 gatcacgaat tggagaaaga gtgtttgaag aaaatgattg cttatggggt agatggtttg
     8401 atcgttgagc ctacgaaaag taatcagtat aacccaaact tagcacttta tgttgctttg
     8461 agagaacaag gcattccgat ggtgatgatc aatgctgctt atgaagagtt aaatgcctct
     8521 tatatttgtt tagatgatgt gaaagtcggt tttatggcag cagaatattt gataaaaaaa
     8581 ggacatcagc gtctgttgtt catcacaaaa atcgacgatc ttcaaggaaa atacaggatg
     8641 aaaggtttta tcagcgcgtg tgaacagtat aatgtgcgtt ttttgacgga agatatcatt
     8701 actttcacca cggaaacacg agaaacaatc gctgaaaaaa cagtacaacg gttgaaagaa
     8761 gaacaagctt tagatggcat catttgctat aacgatcaaa tagctagttt aatgctagat
     8821 aagttgacag aaaatggcta tcatattcca gatgacctat cgatcgtagg aacagacgat
     8881 tcttctttaa gccatttagg gaataaaaaa ttgacaacat tgacgcatcc aaaagaaaag
     8941 ttgggacaag atgccgcacg gtggatcgtc acaacgattg aaaaaggaca ggcacagcca
     9001 aatattctct atgaacctgt actagttgaa cgggaaacag tgaaagaaaa cgaaaaataa
     9061 gagacgggtt tttaccgcct cttattgctc ctacggttac tcgtcgcaat gtttgtgaga
     9121 gcttttgtca tcgctcaagg ctgaacgttt ctttcaccac ctctccacta aacggtgttc
     9181 tcctaccaac tggtcacaag tcacaatatt catcaaacat gagaagctgt tttttgaaat
     9241 tgttccttgt gactggaaac taagcggttc cttcttattt cttgaagctg accacttctg
     9301 tcacaacctc tttaagaatg atggatagaa atacgtttag gataagtttt tttagatgca
     9361 ctttgttcga tttcctctaa agatttccca cgagtttctg gtacgatctc ttgaataaat
     9421 aaaaccccta aaaagcagat gatcccaaaa atgccgaaaa cgatttcttg agacatagaa
     9481 gcagtcatca caggaaaaag caagccaact aggaaagaac caatccaatt aaaagaagag
     9541 gctaggccag atgctcgccc gcggacagct agtgggaata tttcgcctac gattacccat
     9601 gtcagtggag cccatgtgca agaataaaat gcgacataaa tacataaaaa caagagaatc
     9661 aacaacgaat ttgtatgagc atccagtaca gttcctaaaa cagctggtag gatgaaagaa
     9721 agtcccatca cgctaccgcc tattttcaat aaagttcgcc gattgaactt gtctgcaata
     9781 accaaaaaga gcaaagaacc agcaactaaa atgattcctt ggatgatcgg ccacatcaat
     9841 gcatcgctcg ctgcatgccc agtcgctttt tctacgatca gcggaatata gtaaaaaata
     9901 gcattagctc cttgaaattg ctggaatact gctacaccaa taccggcaat gactaaggaa
     9961 cgatattttt ctaaaaacaa agtatgccaa gaagttccgg ttgtcttttc ttgtttgaca
    10021 ttcagctgga tttgttcgaa ttcctgttca gcttcttttg gtgtccgaat atagttcaat
    10081 actctttttg cctcttccaa gcggccagat tgcataagaa aacgcggtga ctctggtaga
    10141 gctaaaacac cgaaaaataa aatcagcgcg gggacagcag cgagccctaa catcacgcgc
    10201 caagccatcg tttcaggcaa cccttttagc aaataatctg caacatagga aaggagcatg
    10261 ccagaagcaa tcatcacttg attgatccct gacaatcttc cccttagtct agcaggagcc
    10321 atttcagaca tataagcagg aactagggca gatgcagcgc caacagctaa acctagaaga
    10381 atccttgctg caatcagaaa ataagaacca ttgtgagggg cagatgcaga aaggacagaa
    10441 ccagccataa aaataagtgc agaaagaaga atcatttttc gtcgtccaag cttatcagaa
    10501 agaagaccag ataaagagcc gccaaggatc gcacctaaca tgaccgaaga cgtgatccat
    10561 cctatcaaag aagctttacc tgccaaaccc caatcatgct gtaaaaaagg taaggcgccg
    10621 gtcataacac caatatcata tccaaataga atgccgccaa atgcgccaaa aaaataaata
    10681 aacgatgagg aaactttttt ttcttttttc ataagtattc tctccttcta gaatttcgag
    10741 aaggcggttg atcagatttt gtaagcaatt tcattttata acttatgtac gtacaaaaca
    10801 agtgtttttt attgtattct tgtaaataac tgcttgacat gtacgtacaa aagtatacaa
    10861 taatcattgt aagcgctact aaaaagacgg tttgatcagg tttgacgaga aggagggagt
    10921 taaatcatta tgaaggaagt aaatgcgcaa agtatgttag atgtcataga tggtcgtaca
    10981 tctttaggga ttgagtttgg atcaactagg atcaaagctg tattgattga ttcatctttt
    11041 actcctattg ccgctggtag ttatgagtgg gaaaacaaac tagaaaatgg tatctggacc
    11101 tattcattag aagatatttg gaaaggactg cgcgtcagtt accgaaaaat ggcagcagaa
    11161 gtattcgaaa tctatggcga gaatcttacg acaatcgggt cgattgggtt cagtgcaatg
    11221 atgcacggtt atctagcatt tgatagcaag gaacagttac tagtaccatt tcgtacttgg
    11281 cgaaatacaa tcactgaaga agcagaacga atattaacgg aaaaatttca atacaatatt
    11341 ccgcagagat ggagtatcgc tcatttgtat caatctattt tgaataaaga agaacatgtg
    11401 aaagaaatta attttttgac aacgcttgca ggttatgtcc attggcagct tactggtgag
    11461 aaagtactag gcgtaggaga tgcatcaggc atgtttccta tcgatccttc aacgaaaacc
    11521 taccatcaag aaatgaaaaa tatttttggt cagttaatcg aaaaagcgca cgtagcgatt
    11581 gacttggatc acattttgcc taaagttcta gtagcaggag aagaagctgg caagttaagc
    11641 gaatcagggg cttatctgat cgaccctacg ggaaatttga gaccaggaat cccggtttgt
    11701 ccgccagaag gggatgcagg aaccggtatg gtcgctacga acagtgtagc caaagggaca
    11761 gggaacattt cggctggtac ttccgccttc gctatgatcg tattagaaaa agaactagaa
    11821 aatgtccatc cggaaatcga catggtcact acaccttcag gagaactagt aggcatggtt
    11881 catacgaata actgctcttc ggatatcaat gcttgggtca gtatttttga agaatttacc
    11941 aattctttgg gtatggagat aagcagagat aaattattca cggtattatt caacaaagcg
    12001 ctagaaggcg atacggattg cgggggatta ctttcttatg gttatttttc cggtgaaaac
    12061 attacaggag taaatgaagg aaggccatta tttgtacgta caccaggtag ccgttttgat
    12121 ttagcgaatt tcatgcggat ccatttagcc agtgcttttg gtgcaatgag aatcggaatg
    12181 gatatattga aatctgagaa tgtccaaatt gatcgtttag tggggcatgg ggggattttt
    12241 aaaacaccag aagttggaca gcggattcta gcatctgcta tggaggcacc agttacagtg
    12301 atggacacag ccggagaagg tggagcgtgg ggaatcgcgt tattagcagc ctatatgatg
    12361 gatcgacatg ggaagtcttt agaaaacttc ttggcagaag acgtgtttgg acaagaccaa
    12421 ggaatcacca tcacaccttc agcagaagaa atagcaggct atcagatatt tatgagacgt
    12481 taccgtgagg ggatcgacat tgaaaaaaca gcgattcatc aattgcaaga aaaaggagtg
    12541 atagaaaatg ctggaacaac tgaaagaaga agtataccaa gcaaatcttg acttgcctaa
    12601 acacggactc gtcaaatata catggggaaa tgtgagcgct tttgatcctg aaacacgata
    12661 tttcgttatc aaacctagcg gagtcagtta tgaagagatg acagcagacg acatggtcgt
    12721 tgtggacttg gacaatcata tcattgaagg taaactcaat ccctcttcgg atacgcctac
    12781 tcatgctgtt ttatatcgtt catttcctca aatcggagga atcgtccata ctcactcgac
    12841 atgggcgacg atctgggcac aagcaggatt ggatgttcct gcgatgggga cgacgcatgc
    12901 agatactttt tatggatcgg taccttgtgc tcgatttttg acacaacagg aaatcgacag
    12961 cgggtacgaa tatgaaacag gaaaagttat catagaaaca ttcaaagaac gcaaaattga
    13021 tcccttagca attccagggg tacttttaca cggacacggt ccatttactt gggggaaaga
    13081 tgcgcaaagt gcggtaatga acgcagtcgt actagatgaa gtatgcaaga tgaatttatt
    13141 taccagacag ctcaattcat tttcagaaga gctgcctcaa cggattttag ataaacatta
    13201 tttacgaaaa cacggaaaaa atgcgtatta cggacaaaac aaataaaagg agtggctaat
    13261 tatgttgaat attggagaaa aagaattttg gtttgttgta ggatcacagc atttatatgg
    13321 ggaagaagca ctgcgagaag taaagaaaca tgcacaagaa atggtcgatg aactgaatga
    13381 aaatggccaa ttgccttatc caatccgcct tcaagaatta gcagttactg ctgatacgat
    13441 cacaaaaatc atgaaagagg tcaattatcg agaagaagta gcaggtgtga tcacttggat
    13501 gcatactttc tcaccagcaa aaatgtggat ccgcgggaca aagcttctac aaaaaccatt
    13561 gcttcattta gctacgcaat ataacgaaag cattccttgg aaaacgatcg atatggattt
    13621 catgaattta aatcaatccg ctcatggtga ccgcgaatat gggttcatca atgctcgatt
    13681 aaataaacaa aataaaatcg tcgttggcta ctggaaacga cctgagatcc aaaaagagat
    13741 tgctgattgg atggatgttg cagtagccta taatgaaagt tttggaataa aagtggctcg
    13801 ttttggtgac aacatgagaa acgtgggtgt cacagaagga gacaaagtgg aagcacagat
    13861 tcaatttggt tggactgttg attattttgg tatcggagat cttgtacaag taatcgatcg
    13921 cgtatcagac gaagaagtcg atcaattatt tgaagagtac aaggaattgt ataccttcga
    13981 ctatggtgat tatgaagaaa agacatggga ggaacatgta aaagtacaag ctcagcagga
    14041 aatcggtatt cgccgtttct tagaagaagg aggctataac gcttttacga ctaattttga
    14101 agacctgtat ggaatgaaac aactacctgg actagctgta caacgtttga tggcagaagg
    14161 atacggtttt gctggggaag gcgactggaa aactgcagcg attgatcggt tgctgaaaat
    14221 catggctcga gggaaagata caggattcat ggaagattat acttacgagc tagctagtgg
    14281 ccaagaagca atcttggaat cccatatgat ggaagtagat ccgacgcttg ctgcgacgaa
    14341 accaagaatc gttgtttctc ctttatcaat gggagataga gaagatccgg cgcgtcttgt
    14401 atttgatggg aaagcaggag aaggggtagt cgtatcgatg gcagatttcg gtacgcatta
    14461 taaactgctg atcaatgaag tagaagcctt tgaaccaacg actgaagcac ctaatttacc
    14521 tgttgcacgt gtgttatgga aaactaaacc gaatttccat gaaggtgtcc attcatggat
    14581 tcaggcaggt ggcggtcatc ataccgttgt ctcgctaaat ctaacaacag atcaaattga
    14641 aacgtgggca aaattagttg agttggaaac agtggttatt agataaaaag aggttgtgaa
    14701 aaagccactt agttttaaga aataagaaga gattgacgaa atttgctttt caactttttt
    14761 gaaatttagt cttattatca atgtcagcta acggaacacc atttatcaag atatctcacc
    14821 ctcaatattt tgctctcgaa aataaggctt atggcagtaa cttgtcatag ccttattttt
    14881 atcactcagc ctactctgct ctaactgctc tttttgcttc taggaagtca aatggtaaac
    14941 agttgtctaa ttgcagaaat ttcgagatac ttgttagtaa ggagggggtt ttatgtatca
    15001 cgttcatttg attgatgcta ttccagaaca agagatagaa aagttacaag gggaatattt
    15061 caaaataata gatgatttgc cagccgaagg aatagttatt cgtagtacga aagtgaaaga
    15121 tgaatggctt acaccagatt tgctagcaat ttcaagagct ggtgttgggg tgaacacgat
    15181 caacgttgaa aaagcttctg aaaacgggac gatcgtcatg aatacaccag gtgtaaatgc
    15241 taatgcagta aaagagcttg ttttgtgctg tttgctctta tcttcccgtc cgataatcga
    15301 agcaagtcgt atggtacaaa cactaacagg tcctaatatt ttagaacagg cagaaaacaa
    15361 aagaagtgct tatgtgggac gagaattgca aggcaaaacc atcggtcttc ttggtcttgg
    15421 agcgataggc acaaaagtag cgctaagttg ctatagctta gggatggatg tcttgggtta
    15481 ttctatccgc gatgcccagt tggattatgt tcggcaagca gatttagaaa cagttctttc
    15541 tacctcggat tacatcgtag tcatgctgcc gttgacagaa gatacaaaag gattgatcga
    15601 tcaggcgaat atagaaaaaa tgaaaaaaga tgctgtactt atcaatgtag gaagaagtga
    15661 aattgttgat aaatatgctg ttatgcaagc attggaaaaa caacatctgg ctaaatactt
    15721 gactgatttt ccagaagaag agttcttaga aaatgaccgt atacttatgt tgcctcatct
    15781 agggggatcg actcaggaag ctttggcaga cagcggacgt ttggcagttg aagccttaaa
    15841 agattatctt ttgttcggta cggttagaga agcggtcaat tatccatccg cacgaatgtt
    15901 tttccaagcg ccgtttcgat tcactatatt ttatcaaaag aaaaccaata tattagcaga
    15961 gatattttct ctgctgaatg agaacgagct agcgatcgca gatatatcgc gtaaccataa
    16021 gaatgagcat gtctacatat tgattgacat agacagtact gattttgaac agctcaatca
    16081 agtgaaacaa cagctagaaa aaatttctgg agtacgaaag gtccgcttat taaagaaacc
    16141 agaaaggcca taaagaaaga gaatatttgg atttcattaa ttcttctttc cgttctttac
    16201 tttcgaattt cgtacaacta aaataagaga agcaacattc ttttattgat agaaatgaag
    16261 gagcaagcat ttgaaaagat atataagcag actttcgacc aaacagaaaa agattttaaa
    16321 aggaacagca gcaatcatca gtatcttttt tcttgttgtt tttagtaatt tatttttgca
    16381 gtggtgtcaa aatgatctat ccgtagacct tgcgttgaaa tttgcttttt cttggcatac
    16441 ggaaaaattc ttcttagcct gtctcgtctt gctgattatt cttatttttc tgattgcact
    16501 agcaggatct gtccctttag gttcccttac gtatgtcgta gccatcggtg tattaggttt
    16561 tgcaaattat atgaaaatga gttatcggca agaaccgatt tatccagacg atttgaagat
    16621 gatcactgaa atcggattgc tgaaagatat gaccggcact atgctgttta cagtgatctt
    16681 agctgccgca ggaaccgtga taggattgtt ttgctggtac atgtttcgaa gcctgaaaaa
    16741 aggaaggcga ttccaattga tccgattaac gacactgctt gtggcaattg gactgctagg
    16801 gtatatcagt aatttcaaca atcccgataa tttgttgcga aaagcatata ataaaacggc
    16861 actatggata ccttacagcc agaaaatgaa ctactataac actggtttta tcggtgggtt
    16921 tttgtacaat ctgaaggtag aaccaatgga tgaacctgaa ggatattcga aagaaaagat
    16981 caaagaaatc actgaaaaat accagaagct agccgatgaa aaaaataaaa ctgtagagga
    17041 agagtcgccg aacatcgtgt tcgtgatgag tgaaagtttc tcagatccta gtcgattaaa
    17101 cggcgtagaa gtatctggtg agccattggc agattattat gaagtagctg atcaaacata
    17161 tagtggcaat atgctttctc aaaactatgg cggtggaaca gcaaatatcg aatttgaagc
    17221 attgactggt ttttctatgg cgttatttaa tgcgcaactc accacccctt acacgatgct
    17281 tgtaccaaaa atggatcagc ttccttctat cgtttcagcg ttaaacgcac aatcatacca
    17341 gacgacagcg atccatccat acaatacttc gatgtataaa agagaggatg tttatcagac
    17401 attaggattt gaccagttca tcagcgagcg tacgatgacc tatacggata cgatagagaa
    17461 taatccatat atttctgatg agtcagctta caaagaagta atgacacttc tcaaggaaga
    17521 aaaaacacct cagttcattc atttagtcac gatgcagaca catatgccct acgatggaaa
    17581 gtatgataat ctagactatc ctgcaaaaac ttcagatgga agtgggacac ttgacttaga
    17641 gaattatctg caagatattt cctatagcag cacggcactg aagcagttca cggaagaatt
    17701 gaagaatctt tctagaagga ctttagttgt tttttggggg gatcatcttc cagggatcta
    17761 ttcagatacg atccaagcaa aaaatgacaa acaaacattg catgaaacac aatttcttat
    17821 gttcgacagt aaaggagaat tagaaaagca aacgactcac gatgcaatca ccagtccgtt
    17881 ttattttgca gcgaatctaa tggaacagac gaatcagaca acaaatggct tttatcagct
    17941 gcttttatca ttagaacagg aacttccggc gtttgaacga gaactgtact atcaaaacgg
    18001 acagtggtat aaagaagcgc aattcaatcg ctcgcaacaa gagatttacg acgaatatca
    18061 attgatccaa tatgacattg tcgcagggaa acaatatagt ttagcagatg gtttctttga
    18121 gcacgaataa gaatggcgag ctgtatagaa accattaaat tttttcaagt acttgtcatt
    18181 tgaggataaa aacaagtaga ataaataata acaacaatgg agaaagtagg aaaacgaaca
    18241 tgttaccgaa tattggctat tatgtgcagt ccatcgatca aatcgtcaag gaaacagaag
    18301 caatcggaga aggaatgcat ccgaaatatg aagtgatcag aaacgcaatt gatgaaggaa
    18361 aaacaagtga actggacaaa tcgactcttg aagagatcat cctgttattt gaagaaggaa
    18421 caaaaaaata tcgggcaatg cttatgaaag tcagtacttt gcgtccacca gcaaaagcaa
    18481 tgggcattca taaaaatttt gaaaaagctt atctttcata tgtagcaggc tgtgaagaaa
    18541 tgatccaaag tatcgatctt gaatcagggg tagatgaaga tctattcaat acatctgaag
    18601 caaaacaaga ccaagcgaca gaccaattga cagctgccat ccaaaaaatg actaatttgt
    18661 tgttgaaaaa ataaaagaaa tctttgataa caataaaaac tagagttatc taatataaat
    18721 gtaatattac ctttatgata gagtgatcaa aatcctttat gataatgaca tacctataac
    18781 caacaacaac cttgtactta ctcaaaatcg agtaaatact ccttttccgt cgagtctaac
    18841 catctcggcg gttttttatt tgtagaaaag aacatcagga tagacggaaa agtcttgtcc
    18901 tgatgttctt ttcagatagt atggaaagct gattagtttt ctacattctg tacatattgt
    18961 tcaaagtagt tctgtaaatc ggctttgagt ttctgatatt cctcttcgct atactcttta
    19021 tcttcgattt tcccatcaaa gaaaggaata tttagttctt cccgtagtct gtctaatacg
    19081 tcttgctgat tcatttcatt ctccttttct tactaacgat caaaataaac acagacacat
    19141 tctggtacat agacatcttt tttcagtaat tccagtaatc ctgtttcagg atgacggcga
    19201 tataatgtca gattatcggt gttctgattg gcacacacga caaaattatt gcttggatct
    19261 agtgcgaaat cgcggggaaa atccccttct gttgagatga tttgcttgtt ttgaatgtgg
    19321 gaaccatctt caaaaactgc gaatacagca atcgagttat gtcctcggtt agaagcataa
    19381 aggaacttgc catcatttga aatgcggata gctgcccctc cattttcacc gtcgaattct
    19441 tctggcaagg tcgatatttt ctgtaattct tcaaaagcac ctgtttcttt attataagaa
    19501 agcacgcgga ctgtgctgtc taattcacca aataaataag cgaattgtcc atttggatga
    19561 aagacaagat ggcgagggcc tgttcctggc tctgcagtga aggtagttac aagattcaat
    19621 ttgccgtttt cggacacatc ataagtatac acacgatctg taccaagatc acatacgact
    19681 aatcgttggt caggagtcag atccgtatag tggacatgcg catgatcttg gtttttatga
    19741 ggacctgtcg cttcttgatg gaatattgaa tctgctgctt tcagttcgcc atctcctaat
    19801 atatggtaga catttacttc acctttatga tagttcgccc cgtatagtaa ttgacgtgct
    19861 tcatcaaccg ctacataaca caaaggagcg ccagactctg ttaccgcatt caaaaaatga
    19921 aaatccttat cgtacgcacc ggcaccacct aatccatcca cactagtaac tgtgtaaaga
    19981 ttgccagctt tactttttgc tacataggta gggctatttt cttcaacagc taatgaaagg
    20041 ttggctaatt cttctttttc tgtatcaagg tcgatcgtat agattccttt actttcgcga
    20101 cgcgtatagg ttcctaaaat tatcttttcg atcatatgag ctgattcctc ctaattgtaa
    20161 tgaaatagtt ttcgttttta ttctatcaca taattattcc acacggctaa agaatgagaa
    20221 tgtgttatac tgtttttgaa agcaattaaa ggagagagtg caatgattca ggcaaagatt
    20281 acagaaattg ggacagaagc aataaacaag gaagaaccca tgctgatttt gttcgataaa
    20341 acagcaacag ctgccttacg aaaatattcg gtgattcaag aagtatcaaa agacgaacct
    20401 ttttctttga aagcaggcgg aacaattgct tttgatgatc aggtatatac gatcgattat
    20461 gtcggtccga tggccaatgg gaatttaaag acagtagctc acgtatcatt gatttttgat
    20521 gcttgtccag aagaaggaca aattgcaaat ggaatctatc ttcatccgca tagactgcct
    20581 gctattggtt tgggaagcca aattttttat aactaacagg aggaattatg gaaggaaaaa
    20641 ataagaagaa cagaccggta tcgtggtttt ggcgctggtt tttaaataat caagtagtca
    20701 cagccttact tgttgtattg ttaatattgc tgatcgtctt tttatttaca aaagtttcat
    20761 atttgtttgc accaatctgg caatttttag caatcgtagg tctgcctatc attttagcag
    20821 gaatcttgta ttacttgatg aatccggtag ttgattactt tgaaaagcga aaagtaccaa
    20881 ggctttacag tatcattggg ctgtttgttt tggttgttgc actaatcgca tgggggagcg
    20941 tagtgatcat ccctaagatt caggaacaaa cagtgagctt catcagtaac ttccctcaat
    21001 atgtagatac gattgataat aaattaaccg agattttgcg ggacccgctg ttcaaccaat
    21061 ttcgcgaaca gttggaaaca gcaggtgata agttcatgaa ttcggcaggc gatatgatcc
    21121 aagatatatc taagagtaca gttcagagtc ttggaagttt tgtcggcgcg gtagcgacta
    21181 ttttagtggc tctgctgacg atgccgttta tcttatttta tctattgaaa gacggtaaac
    21241 aattagcacc ttattttgtc aaatttttac cgacacgcat gcaaaaaccg acgttgaatg
    21301 tgctgagtga aatgaatagt caagtttctt cttatataag aggacaacta actgtggctt
    21361 ttgctgtggc aatcatgttt atgattggtt ttgcggtcat tggtttagag tatgctgtca
    21421 cgttggggat tattgcgggt tttttaaacc tgatccctta cttaggatca tttttagcga
    21481 tgattccggc tatcttttta gccattgtag caggtccatt catgatcatc aaagtactga
    21541 tcgtttttgc agtagagcag acaatagaag gacgcttgat ctctccatta gtactaggga
    21601 atcagttagc aatccatcca gtaacgattt tattagttct gctgacttct gggaaattgt
    21661 tcggaatcat cggtgtgatc ttggggattc ctgtttatgc agcagctaaa gtagtgatta
    21721 ctcatgtttt tgaatggtac acaacaatct cttctcttta tgaagaaaat ccgttgaaaa
    21781 agcagaaaga ataacaaaaa gagacagatg ggaaaatatc caaggcacaa atgctgcttt
    21841 gaagattttt tcctctgtct cttttgtatg catttatctc aatttttaaa atgatttacc
    21901 taaagcagca gctttatcta atgcattttg catcaactca tctgcacggt cagggtcata
    21961 atcgattcct tcgatgaata gctgatctac ttgatcgatg cctacaaagt ttaaaatacc
    22021 ttttacatat tgagaggcaa agtcttgtcc gttgtataca ccgccattag attggatgtg
    22081 cagtgctttt ttacctgtca ctaatccttc tggcccgttt tctgtatagc ggaacgtttt
    22141 tccagctaca ttgacggtat cgaaccaagc ttttaatttt gttgggatat ttagattcca
    22201 taaggcgttt gcgataacga ttttatcagc agcaaggaac tgatcagtca attcattgaa
    22261 acgagcaatt ttttgttgtt gactttctga taaggaagtg aattctgcac cagcacgtaa
    22321 tgcttcccaa cctgataata attcttcatc gatttccgga atatcatcgc gataaagttc
    22381 caaaacagtc acttcatcat ctggatgatt ttctttataa cttgttaaaa aagacgtcaa
    22441 tgccttaact gtgcgcgatt cttctgcagt taggggatga cctttgacta ctaaaacaga
    22501 tgccatatgc ttaccttctc cttttattgc tatgattgtt ataactatga ctaacaaaat
    22561 cagtttactc gaaaaagaaa aggctatcaa gtcatattga taaggttgaa gaataaataa
    22621 ctaaaaaaag acagggtata aaagaggctg tgacacgatt ttttgtcaca gcctcttttg
    22681 aatctaacaa gcaatattca agcaacactc gactgaagag cttccatccc accaatgata
    22741 taaacagcac gaaaaccatt agcagtaagt tgatcggcta atgcttctga tcgcctgcct
    22801 gtatagctta gaatataata aagtttcttt ggatctagat tttttaattg tcttggcaag
    22861 atagcagcgg ggatagaaag cgagtggctt aagtggtttt ttgtgtaatc taaggtattt
    22921 cttgtatcta aaacgatgct gtcagcatcg agaggcaatt tattgaagtc ttggacacta
    22981 attgattggg ccatttatct catcctttgt ttagtatgtg gctattttaa tgggcaactc
    23041 tcattaattc aaatggaagg attcctctta tcactttttt aatgtatcgg atgataatga
    23101 aaaaaactat gcaaatgtct tgcaaaacag gtaggaggtg ttataatgac atagcgtcat
    23161 atgacaatgt gtcatcttga aagaaaggag aatccatatg ccgacacaga catttttcca
    23221 tttgcctaaa gaaaaacaaa aaagactaat cgaggctgcg agaatcgagt tttctagagt
    23281 acccttaaaa gaagcttcga ttgctaatat cgtcaaatta gcggatatcc ctagaggcag
    23341 tttctatcag tattttgaag ataaagagga tctttatttt tattactttg agacaatgag
    23401 acgtgacagc tctaaagata tgttacaact aatgaaagaa tccgatggtg atctattcaa
    23461 aggattcgaa tcctattttt ctaaaatgat ttatgagatt ttggaaggcg agaatgcttc
    23521 tttttatcgt aacttgttta tgaacatgga ttaccgtgcc tcaagaaaag tcgctcctca
    23581 tacgagtcgc caaaaaacgc ctcacgatcc acataaagaa cacaaacaag atgcaaaaga
    23641 gttgcttgaa ttgatcgatc ggacgaagct gaaagtaaaa aatgatcatg aattggaatt
    23701 actgatccaa ttagtgatga atactgtatt tacaacaatt gcgcattcgt atcgcttatt
    23761 ttcaacatct gaagattact cagtagagca agcaatcagt gaattcaatt tgaaactttc
    23821 ttggctgaaa aacggagtat ataaatagaa agaagggaac tagatgctaa aaatcgtaaa
    23881 acggatctcc ttgacttcag caattgcagc tgcagttttc atggtgatcc aagtactggc
    23941 agatttatat ttgccgacac ttacctccaa tattattgat aaaggggtag cgcaaggaga
    24001 tgtcgattat atttggcata caggttttgt gatgatcggc ttttctttga tcagtatctt
    24061 agctgctatc gcaaatacat tttttgctac tcgtgaatca cagaaactgg gtaagcaatt
    24121 gcgtacggat gtctataaaa aatcagagag tctgacaaaa gatgcgtttg ataaatatgg
    24181 aacggcctct ttgatcacaa gaacgaccaa tgatgtgact caaatccaaa tggttacaca
    24241 aatgttttta cgtatgatga tcaatgcacc aatcacgttg attggcgcga gcatacttgc
    24301 ttatcaaaaa gatcatcagt tgacaaaaat cttcttagtt gtcatcccag taatgattat
    24361 tttgatcggc ggaatcatgt actttgctgt tccattattc aaaagtatgc aaaagaaaac
    24421 tgaccgttta aacctggtat ttcgagaagg attgacaggg gtcagagtga ttcgagcatt
    24481 cgataagaca cgctttgaag aaaatcgttt tgatttagcg aataaagatt atacaaatac
    24541 ggcaatcaaa gttaatacga tcgtcgcatt gatgatgcca atgatgacat tgatcatgag
    24601 cggtacaaac gtagcaatca cttggtttgg tggacactac atagcagata tgactttgga
    24661 agtcggtaat ctgattgcat ttatgactta tgcgatgcag atcttgatca gctttatgat
    24721 gctttccgct atttttatca tggtgcctcg tgcacaagct tcagctgacc gtattaatga
    24781 agtgttggat gaaaaaatcg gtatccatga ccctgaaaat cctaaaacag tttcttttgc
    24841 tggaaaaaac gcaacattgg ccttcaatca tgtcaattat cgatatcatg gagcagaaaa
    24901 acttgcattg gaagatattg atttccaagc caagtcgggc gagatcgtcg caatcatagg
    24961 ggggactggt tcaggtaaaa cgaccttagt caatttgatc cctcgattat atgacatcga
    25021 atctggttcg atccaaataa atggaacgga cattagtgac atgactcaat ataatttgcg
    25081 agaattaatg ggctttgttc ctcaaaaagc agttctgttc tctggaacga tccgagacaa
    25141 tatgcagtat ggtaagccgg atgcaacaga tgaaatgatt tggaaagcat tagaaatcgc
    25201 acaagctaaa gattttgttt cagagatgga agatggctta gatagccatg tggaacaagg
    25261 aggaggaaac ttctctggtg gacaaagaca gcgtctggca attgcacggg cattagtcaa
    25321 aacagcagat atctatgtgt ttgatgattc cttctcagcg ttggatttca aaacggatgc
    25381 caatcttcgc caagcattga aaacgaatat gaaagaaagt atcaccgttt tagttgctca
    25441 acgagtaagt acagtaatgg atgcagatat gatccttgtg ctagatgaag gaaaactggt
    25501 tggcaaagga acacatgaag aactattagc aacaaacgaa acttatcaag aaatcgttca
    25561 ttcacaacta agagaggagg acttagcatg agtaaaacaa acacacgtcc ctctcatgga
    25621 ccagcaggcg gtccaggaca tggtggacct ggcagaaatc tcggtgtaaa aggtgaaaag
    25681 cctaaaaatt tctggggaac ggtacgccga ttgtttggct atatgtctaa acgtatggtt
    25741 gcgatcatcg ctgtatttgt actagcaata gcagccgtga tcttccaaat ccaaacacca
    25801 aaggtcttgg ggaaagcaac cacagaaata ttcaaaggtg taatggctgg cgctcaacag
    25861 atgcaacaag gccaacaagt aacaaaattt ccgatagact ttgataaaat cgcgcagatc
    25921 attgcgacag taatcgtgat gtatttgatt tctgctgtat tcaacttttt acagcaattt
    25981 attatgacac gtgtttccca acgaacagtt tatgaattac gtcatgattt ggaagccaaa
    26041 atgaatcgtg taccgatttc ctattatgat acacatacaa atggggatat catgtctcga
    26101 gcaatcaatg atatggataa catcgcaagc acgctgcagc aaaatttgac tcaatttgta
    26161 actagtgcag tcacattgat tggggtcatc gtgatgatgc tttcaatcag ctggcaattg
    26221 acgttagtcg cgttattgat gatcccttta agcttgatca tcgtgatgat cgtagcgccg
    26281 aaatcacaag tgtttttcgc agatcagcaa aaaagtttag gtttattgaa taaccaagta
    26341 gaagaaacat atggtggtca taccattgtt aaaacattca atcatactga aaaagaccag
    26401 gaagtatttg aaaaagaaaa tcagaatctg tatgccgctg gttggaaagc tcaatttatt
    26461 tcggcaatca tcatgccgtt gatgaatttc gttaaaaatc taggctatgt gttcgtcgct
    26521 gtactaggcg gagtaaaagt tgcaaatggg aacatgacat taggggatgt tcaagcgttc
    26581 ttgcaatata caacacagtt ctctcaacca atcactcaac ttgcaagttt gatgaacact
    26641 atccaatcaa cagttgcttc agccgaacgg gtatttgaag tgttggatga agaagagatg
    26701 tccaatgaaa aatctggcct tccagaagaa aaagatacac catacaaagt tcgttttgaa
    26761 aatgttcaat tcggctacac accagataac ttgctaatga ctgattttaa cttggatgtc
    26821 aaacctggtg agatggtcgc tattgtaggt ccgactggtg cagggaagac gacactgatc
    26881 aaccttcttg aacggttcta cgatgtttct ggaggtagta ttcgttacga tggtgtagat
    26941 acccgtgatc tttcacgtga tgaattgcgc gcacagtttt caatggtact gcaagatact
    27001 tggctgttta caggaagtat ctatgacaat atcaaatatg ggaacgacga tgcaacagac
    27061 gaacaaatca ttgaggctgc taaagctgct cacgtagacg attttgttcg taaattgcca
    27121 gaaggatata acactgtatt aaacgaggat gcaagcaata tttcgcaagg gcagcgtcag
    27181 ctaatcacaa ttgcgcgggc attcttagca aatccagatg tcctgatcct tgatgaagca
    27241 acatctagtg tagatacacg aacagaaata ctgattcaaa aagcgatgaa ccgcctattg
    27301 gaaaaccgga caagtttcgt tgttgctcac cgcttgtcta caatacgcga tgcagacaac
    27361 attattgtga tgaatcatgg ttcgatcgta gaaacaggaa accacgatac attgatggaa
    27421 aaagacggat tctatgcaga tctttataac agtcagtttt ctgaagaaga agcttcatag
    27481 aatagtacaa atttattatt ttttttaggg gctgtgacaa aagtcgtgtc acagtccctt
    27541 tttccgaata aacggtgttc aatcagctgt ccttcggtat tagctcaaaa aacggcaaaa
    27601 tatgagaagc tatttttccg ttttgtatct ctaatactca ggtcaaaccg ctgattttac
    27661 aacctcttcc ttaaacggtg ttctcctgcc aactcctcgg tcacaagtca caatagtcaa
    27721 caaacctgag aagctgtttt ttgaaattgc tccttgtgac tcggagttaa acggcaggct
    27781 cacaacctct tggtaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg
    27841 caaaaatgta gaaagccatt ttcacaaaat ttttcttact actcgaaact aagcgacttt
    27901 ttcacaacct cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc
    27961 aaacgaaaaa taggaggagc tatttttgtt tggctgttct tattacttga tgcagaacag
    28021 ctttttcaca acctcttttt ataagagttt ttactcatta atttacaaaa aacaagcaaa
    28081 atgcgaattt tctcacattt tttttaacaa aatcaaagtt attgcttgaa agaagaagaa
    28141 tccatcgtta aaataaagct tgacgtagag gggagcaaga aatatgaaag attttcaatt
    28201 gggggctgcc acctatttaa aaagtgtagc cattcgggtg aaagaccgag ataaaatgat
    28261 tgatttttac aagaacgttg ttggctttga tttgaaaaga gaagaaaacg aattagccat
    28321 tttaggaaca gaagaagctg gaagtgaaat gttgctgctg gaagaaagtc ctagagcgaa
    28381 tgatcacatg ggggtgatca aaaagctgaa ccgtttttca ttgattatcc ctagcacaga
    28441 agaaatggca gatatccttt gtcgtatccg taaaatgaat tatccaatca aagaagcttt
    28501 agaagatcat ggacgtatgg ggattttgct cgtagaccca gaagagaatg aattggaaat
    28561 ttaccatgaa gagaataaga acgaccaaag agaaattcca gaaacaatgg atcaagaagc
    28621 attattaaga aaatctgaag aaaaattcag taaactatca acaggttctt attttgaaaa
    28681 aatccattta aatgttacta acttaacaaa agaacgccaa tttcttaatc atgtacttgg
    28741 tttgaaggta cataatgaag aagagggcat ccatgtattg aatgaaggag atttccatgt
    28801 tggtttgaca gaagctagcg gaggcgcaat tgatttgcct acagatgaag tgattggact
    28861 tgattttctg aaattcatga tcgatactga aagcatggaa ggactgattg ctcacttgca
    28921 gcaactatca gttgatttct tcttagacaa aaagcacaca gtactcacaa tctatgatgc
    28981 tataggaatc gaatggtggt tcgtcagtga gccaaaagaa aagaataaaa ctgacattcg
    29041 atgcgacgct taactttttc tttcttaacg attgtcgcat gtctcgtcat tttatgaacg
    29101 gtcatactat cgcttattct agagatagaa agagaaaata gtataccggg ggaaactgcg
    29161 cggtttttcg cgcagttttt ttgtgtaaaa aaggttgatt cctcatagat taagctttaa
    29221 gcttggactg taagcatctg atagaaaagc aggagaaaaa gatgtatcca cacacgaaac
    29281 agcttatttt gaaaaagatg gaagaaggat tttatccagg tgtagcagtc gccttcataa
    29341 aagaaaatca agtagaaaca tttacagccg gctttgccca agtcgttcct gaaaagcagc
    29401 tgatgacaga agatctgttg tttgatgcag cctcactgac gaaagtcgtt gcaaccactt
    29461 cattagtttt acgtctatta gaagatggaa agatagaact cgatcaaccg cttcataaat
    29521 atcttcctga atttgaaaat cagacaatca ctttgcgtca tttactgaca catacttctg
    29581 atatcaaaac atggatacca aacagagata agctatctca ggaagaattg atcgccgctt
    29641 atttgaagct agaaccaggt gagcaactag gaaaagaagt aaaatataca gatgccggta
    29701 cgattcttct tgggttgatg ctagaggaaa tttaccaaga aactgcagtg gaaatctttc
    29761 aagaacaagt attggaacca ttaggaatga tgaacagctt atttcttcct tttccttcca
    29821 aaaaaatcgt tcccacagaa caactgcctt cgggtgaggt tttgcgtgga caaactcatg
    29881 atccgaaagc aagagttttg gctgaacatg caggtaacgc gggtctcttt attgatttga
    29941 aagacagcat tacttttgtc caaatgtatt tgaacaaggg aaaaatccgt aatggtcgtt
    30001 ttttagaaga agagacgatt gattctttac tgcaagatca aacaccgaaa aaaaatggct
    30061 atcgttcttt aggatgggac ttaaaaaagg atgtagaaga cggtcatgcg attctttttc
    30121 atactggtta tacaggtacg tttttcttga ttgatataaa aacacaagaa gcgttgatat
    30181 ttttgtcaaa tcgcgtacat cctgtcgatc atcgtgcgga gtatctcata tgtcgagatg
    30241 aaattgttga aaactttttg aaagagaaag cggtattacc gaaataatgc tataatagct
    30301 atgaaaaatg agaggagttt gaattatgca ggaacctatg tttttaaaac cagtttttca
    30361 agaaaaaatt tggggaggaa gtcgtttacg ttcagtcttt ggttttgata tccctaacga
    30421 taaaatcgga gaagattggg cgatcagtgc gcatcctcat ggtgtcagtg tagtagaaaa
    30481 tggcgaattc aaagggaaaa gattagatga actatggtca gaacacaagg aattgtttgg
    30541 ccatccttca gaaccagtgt ttccattatt gatcaagatc ttagacgcag aagatgatct
    30601 atctgtacaa gtacatccag atgatgccta tgggatgaaa catgaaggtg aattaggaaa
    30661 aactgaatgc tggtatatca tcgatgctga acctggtgca gaaatcatct atggtcatca
    30721 tgcaaagaca agagaagaac tagctgagat gatcaaagat gggcgctggg atgacctttt
    30781 gaagaaagta ccagttaaaa aaggtgattt cttttatgta ccaagcggaa cgatccatgc
    30841 tattggtaaa ggaatcatga ttttagaaac acagcaaagt tctgacacga cttaccgcgt
    30901 gtatgactac gacagaaaag atgcgcaagg acaaacaaga gagctgcata tccaacaatc
    30961 gatcgatgta acaactgtcc ctgcgaaaac accagaatta caaatcaaag aagtccgaaa
    31021 agggaattca tcgatcgtca cttatttaga aactgagttt ttcaatgtat atgagtggga
    31081 tattaaagga atcacatctt tcaaaaaaca agccccttat acattgatga cggtaatcga
    31141 aggagcagga gaccttgtcg ttgacggaaa aacatatcca ttagaaaaag gaacaagctg
    31201 tattattcca aatggcgttt ctgagtgggc ggtccaaggt gaactagcaa tcattgcgtc
    31261 tgaaccaggc aaatagttcc cagatagctg aaagcagcag acggtttttc gtttgctgct
    31321 ttttctacat aaaatccgtc catcaataaa tagaagtctg gtgaaaaaga tggaatacaa
    31381 atggaaagca aacaacagca gtcgcgtgtc tatgaaacaa ctgagaaaag ctggacatca
    31441 cttaaatgaa atgatcatgc caaatctaag tctgtttatc gcttggggaa tcttgtcatt
    31501 agcaacaaaa ggtgtgtctg gcgatttgca caccacatta gaagaggtat gccattggat
    31561 gatccagctg cttttaccca tcttgatcag ttattcaggc gggaaatcgg tagggggaca
    31621 aaaaggggca gtcgccggtg caattgcttc tttagggttg atcgctcgtt ccaatgctcc
    31681 acaaatcgta ggagcgatga ttatcgggcc gcttgccggc atcgtatatg aacaatttgt
    31741 ttctagattc catgtgaaat tcaaggctgg atatgaaatg cttgtcagca acctactagt
    31801 aggactgact ggtagcgtta tttgtataat tggaatcgtt tttattgaac cagttttaaa
    31861 tagtgttcac ttgttgctcg cctccgttgt gtcttggcta gtcacaatga atctgttacc
    31921 acttgtcagt ctgatcttag aacctctaaa agtactattt ttcaataatg caatcaatca
    31981 tggtgtattg acacctttag gaatcgaatt ctcacaaaca gtcgggtact cttccttatt
    32041 tttgatggag gcaaatcctg gacctggttt agggatctta ctttctatcc tttgttttgc
    32101 agaaaaacag gaaaaagtaa atgcgagtgg agcattgatg atccatgcga ttggcgggat
    32161 tcacgaaatc tattttccat ttgtcttgct gcgaccgtat ctatttttag ctgtaatggc
    32221 tggtggggca agcggcacgt tgatatttca atggttagat acgggattag ctgcacctgt
    32281 atcgcctggg tcactactcg tgatttttgc caatgcgcct tatccacaac tagctggggt
    32341 aagtataggg atagcaatca gtacatttgt gaccttcatt tgtgcagcga tactattgaa
    32401 attaccaaat aaagaaaaag agaacaaaac ggtcaaagaa aggggagaaa tcttggaacc
    32461 atctatcatt cattccgtca tttttgcttg cgactcaggt atgggatcaa gtgctatggg
    32521 agccgccctg ctgaaaaaga aattagcagc agaaaaaatc gatctttctg tcggatacag
    32581 ctctatctat gaattgaata accaagtaaa tcagctggtt atcgtgcaac atgaactagc
    32641 gggtcttgca agagaaaaag tacctcaaag tcaaatctat gtactggatc actttttgga
    32701 aacagatatc ttgctgtcac gaataaaaga agcgatccaa tttccaaagc gggaagaaaa
    32761 aaaagagata gcagatatca cagaacaaaa aacgtcgcct gctaatattg tatttttgta
    32821 taaaaacaac agacgcggct cacaaactat gggcatgacg attttaagtc aattagcaaa
    32881 aaaagcgaaa aaacagtttg ttatctcaaa agaatcaata gaaaaaatca aaatccaaga
    32941 agaaacgatt tatatcgcac aaagagaact agctgtaact gaacagctag aagaaaagat
    33001 ccctcggcta gttctagttg aacattgggt agctacgaat gaatacgaaa aactcgtgaa
    33061 gggagaaata taagatgtat ttatcgccca gagaaaaaca attattagca gaatttctca
    33121 gtagcccgtc acctgtatct atccaaaaaa tgatgaattt attgaaggtc agtaaacgga
    33181 ctatttaccg cgaattagat aatcttgaag tctcattgaa atcggtaaat gcttctttga
    33241 aaaaagcagc tagaggctca tttgtcattg aggcatcaga agaagtatta gagcaattaa
    33301 aagaaaatac agatgggaaa agtaatgact tatccactgc taaacgacaa cacgcgattt
    33361 tagccgagtt gttactgacg acagagccgt tgagtcttgc tcatttcatg gaatcatatc
    33421 gcatcagtaa taccactttt tatgcagata tcaaacagtt ggaagaaagt attcgccaac
    33481 tgcctttgga aatcattcgg aaccagggat acgagatcga aggaccggaa aaataccgcc
    33541 ggcttctgac agcgaatgta ttagaattag aaatcaatga atatgaactt tttcatagta
    33601 tttctttcga tgcatcactg aattattttt ttcgattcgt tgatccgcaa catctatctc
    33661 ttgcacgcaa agtaatcggg gaagaattga tccaaaaaaa gaccaatcta agtgatagaa
    33721 agcttgaaca tttggttttg atgctgacat tgacgatgga gcgtgtgacg aaaaatcatt
    33781 tactgacaaa tgagacatat aatagtctgg caaacaaaga attactacag acagctaaac
    33841 gattgttttc acggatcgct tctgagacaa aacaacttta tccagtaaat gagatcgtct
    33901 tttttgcagg attattaaat gacttcacga actcatttga tcagaatttc tttgaagaga
    33961 cgttcgatac acagcttgct tatttagtaa aacaactgat tcaagaagta agtgaagaga
    34021 cgaatgtcca tttttatgaa gatgaaactt tgtacaaaat gctgttgact catttatctg
    34081 gtgtgttttc aagagccgtt ttacaagaag agacactgac caatccgatt ttagaaaaaa
    34141 tcatggttca atacgaggaa gttgcgaatg caatccgtaa agcagtagag cggatttttc
    34201 aagaaaaaaa attatccgaa gaagagatcg cttatatggt ccttcatttt gcgaattcat
    34261 tggaacgaag tccgaaagaa agcaatatca gtattgcagg attttcgcca agtggattag
    34321 cttctactag catgttggaa ctacgattga aaaggtattt tcccttcatc caacaaattc
    34381 atttctatcg gattgctgat ttaaaaaaaa tcgatttaca agaagaatat gatgttgtta
    34441 tttctacatc tattttacca ggatacggtg gaaaatatat tttagtctcc ccgctactgc
    34501 tagaagatga agtcaagcag ctaaaagaag ctttttcgca tattgagaag aaaaaaaggc
    34561 aaaaaattac gtataacagc aataacgagt ggatgataga agatacttat gaggaaacaa
    34621 tgcatttcat ggatcaaatc aatcaattac tcggtcgttt ttttatccaa gaactggata
    34681 atcctgagac gatttccgaa acgattcgca gcttatttcc ttattttcct gaaggactag
    34741 taacggatga agaggtggtt catcgccggc tgcttaagcg atatgaacaa gcacctatcg
    34801 gtattcctca tacaaatatg gggttattcc atacatccag ctcattgatt tctaaaccga
    34861 tcttttgtat tttcaatttg aaacatccgt tgaccattga agggatggat agacagccag
    34921 tcgatttgtc ccgtatgtta gttatgctgg cgccttcgcc aatccaagaa aaagacggga
    34981 ttcttttagg aaagatcagt ggagcaatca tcatgaatga cttgaacacc gagatcttcc
    35041 gctcaggaaa taaagaaatc gtttatcagt tgttagcgaa aatcctgatt gaagaaataa
    35101 agaaatgaag gtgaaaagat gatacagcta ccagcagaaa aaatattgct cgatcaatcc
    35161 tatcaaaatt ggaaagaagc gttgaccgag atcggccagt ggatgaccca acaaggggga
    35221 atcaaaccgg catatatcca atcaatgatc gaaagacaag aaaaagcgag tgtctatatc
    35281 gggaatttcg tagccttgcc acatgggact gacaaagatg aagaagtagt agaagaagga
    35341 atctatttag tccaagtgcc tgatggtgtg gactttggaa caaaagagga accaaaaatc
    35401 gcgacactct tatttgtagt ggctttaaaa aaagaacatc agttagcggt gctccaagat
    35461 ttagcctttt tctgttcaga catcgaccaa gtaatggcgc tcagtgatgc ggaaaccatt
    35521 gaagaagtcc aaatgatttt tgaacatgga catatataac caatgttcac agtaaaaaaa
    35581 gctgccttgc atcaagtaat aacttcaacc aagataaaaa taagaggagc tatttttgtt
    35641 gggaggttct tattactgcg gatgcaagct tttgaaaact atttattcta gaaaaccgtt
    35701 tatttgaaat aggggacggt gacaaggttt ttgtcaccgc ccctattatt tttgtgtttt
    35761 aatagctctt ttttatatag atagtgccca tacctatgaa taataaaacg atactccata
    35821 taccaagtgt caagatacca ttcatgaaag taacctgttc tgaagctagt ggcgagaaaa
    35881 tatctcctcc tatgatcaca ttttgaaaat caacataaga agttggcaag taatgactga
    35941 ttgattctat ccatccaatc gaagaaaaca agcgatactc tgctaagaaa agacaaagaa
    36001 gtgtacctaa aagattgact agagaatgtt caaataaagc agatagaaga aaactgattg
    36061 tacttaaaaa gagcacccac aacaacagga acagaaagct ctggaaaaga tactcataca
    36121 tcggcaggat tcgtatctcc tgaccaatga tcgttcctac aggataacgg aagtctccca
    36181 aaccattttt gattattact agtatagaga cgatcaaaaa tggcatcacg agacttagca
    36241 tagctgcacc ataataaatg gtaaaacgga tgctatgttt ctttaccata ctatccggta
    36301 atagattgac tagagagatt acctgctttc ttttttcata agagatgatg gaagaaagaa
    36361 acaaggcggt tatacataaa atcaaaaaac tagaaatcat tcctgaaaaa atattttcgt
    36421 agtaatttgc taaagagaga tgcgctggca agtcaaaaat agaatatctt tggatctgat
    36481 gttctttcat ataacggagc aactcgacac gtttttgttg ttcaatgatt ggaattcctt
    36541 gtctttgacc agaaatcagt cgatccagta tatctttttc ataatgataa gttgcttcga
    36601 ccatttgctg gacattccct gtgttttttg catgtaagat tgcttcgatc agtttgttgc
    36661 ttttctctaa actttccaca aattctttct cattctcatc ttctttcaac gtttcaatca
    36721 aacgttgcat ctcttcatag ttatgattca gttcatcgaa ttctgtataa gcaagtttat
    36781 gatcaaaaaa aagtgggaat gcaaagagca cgaccaacaa tagaggaaat aaaaagttta
    36841 ctttgttttt tagtaagaga ttgcgttcaa atgtaagtaa agacatagtt acacctcata
    36901 taatttacga taaatagcta aaatctcttc catatttgag gattctgtga cgatttctcc
    36961 attttttaga aaacatgctt tagaacaaat cgattggata ttcgcgatag cgtgggaaga
    37021 aagaagaatc gtcttcccct ctttctgcca ttgtctgatg aggctattca agatatcaat
    37081 acttcctggg tctaatccgt tcaacggttc atcgaataac atgacagatg aatcactgat
    37141 caagtaagca gcaagcaaga catgttgttt cattcctaga gacatttttc caatcggcaa
    37201 agtaaggtac tcagtcatct tcaatcgttg gaaaacagtt tggcattcta ctttgctctg
    37261 ccaatagtat ttgatcaaag tcaaataatc aacagccgat agatagtcgg ataactgatt
    37321 cgttgattct agaaaaaaga aagaccggcg ataatctgta taagcagtag gagagatgtt
    37381 gtgaagggac aattgatcat aagttgttgg cagcagttgg gcaatgccat tcagcaaagt
    37441 tgttttgcca aatccgttcg gggcaatcaa agcagctact tctcctgatt ctactaaaag
    37501 attcaattgt cgaatcacac gttttttacc acgtttcatg ttcatgtttt tgatcagtaa
    37561 tgtcatagat aggcctccaa tataaaattt aataacataa tagaatggaa aacatctaaa
    37621 ttaaatgcta acaaaagtta ttgtttgcat attataaaaa aattaaaata acagttttat
    37681 ttaaaattga agttttctga taccgaaatc tttttatttt gttgacatac tcaaaccttt
    37741 ggcacaaagt ttcgtgccaa agtacgacta tttctctttt tagtaagcgg atacaattag
    37801 agtatcaaaa ggagagaggg agagacaaaa tggaacggac gattcccaaa aacaacattt
    37861 cgctaaaagc gagggtacaa aaattaggca gtactttatc aagcatggtc atgccaaata
    37921 ttggagcact gattgcctgg ggtgtattaa cggctctatt tatcccagat ggttatttac
    37981 caaatgaggc tttcgctacc atggtaagcc caatgctaac ttatctgatt cctttattga
    38041 tcggttatac tggcggaaaa gtaattgctg gtgaccgcgg atcagttgtt ggggcaattg
    38101 caacgatggg tgtgattgtc ggaacagata ttccaatgat gctaggcgcg atgatcatgg
    38161 gacctcttgg tggatatgcc atcaagaaat ttgatcagct attccaaaaa cggatcaaat
    38221 ccggttttga aatgctagtc aataattttt cagcaggtct tatcggtttt ggattagcgt
    38281 tattaggttt ttctgcgatt ggtccagttg tcgatgcatt gactcaagca atggcaaaag
    38341 gtgtagagat cattttatct gcccatttga ttccattgac aagtattttt attgaaccag
    38401 caaaaatctt atttttgaat aatgcaatca atcatgggat tttgacacca ttgggaactg
    38461 aacaagtagc agaggctgga aaatctattc tattcttgct tgaagcaaat cctggtccag
    38521 gacttggcgt attacttgct tttactttgt ttggcaaagg agcagcaaaa tcttcagcac
    38581 caggagccat gatcattcac tttttaggcg ggatccatga aatctatttt ccttacgtta
    38641 tgatgaaacc gttattattc ttatcagtta taggcggagg aatgtcagga agtttcattt
    38701 tccaattgat ggatgctgga ctaagagcgc cagcttctcc agggtcgatc attgcgattc
    38761 tagcgatgac accgttcaat tcttaccttc ctgttatttt gggcgtgatt gttgccgcag
    38821 ctgtttcgtt tggtatttct gcagtaattt tgaaagcgga tgcgaaagat acaaatgaag
    38881 acttcgaaaa atctgttcaa gagacaaagg cagctaagca agcttccaaa ggtgcccaag
    38941 gtattcctca aggagctaca ttctctggcg tacaaaaaat catttttgct tgtgatgcag
    39001 gaatgggatc aagtgcgatg ggcgcttcga tcttgagaaa aaaagtacag gaagcagact
    39061 tgcctcaaac ggtgaccaat caagcaatca gccaactgac agatgactcg aatacattga
    39121 tcgttactca ggtcgaacta caagaaagag caaaacaaaa agcaccgagc gcgcgttttg
    39181 tctcagtaga aaactttttg aattcacctc gatatgatga aatcattcaa gaattaagca
    39241 gcaagcagaa aacatcagtt tctgaaattg agaatgagcc agcagaagag tttctttcag
    39301 caaacatcaa acaaatcaaa gagattctac ttgtccacga tgatcgttcc ggatcagcaa
    39361 caatggggat gaccgtgctc aaagatatat tagacaagaa ccatttaact atcccgttaa
    39421 gaaaagtcaa tatccaagat ttaaaagatg aacctgattc actgatcgta gccaaagaaa
    39481 gtttagcgga aaaagtacag atggctgcac ctcaatccat tcatttatca gtcaacagca
    39541 tggtcaatcc gcaaaaatac gaatcgattg cacaatattt gaaacagaca gcttaaggga
    39601 ggaaaacagc atgcaaagtt tgccattcga catgatcgag ctcaatcgat cttttaaaac
    39661 caaagaagaa gcgatccgct attgcggtca gatgcttgta gaagcagggt gcgttgatga
    39721 ggaatatatc gatgccatgg tagatagaga caaaatgtta tctgtttata tggggaattt
    39781 tatcgcgatt cctcatggaa cagatgaagc aaaaaaatat gtgaaaaaat ccggcatctg
    39841 tgtcgttcaa gtaccagatg gtgtagattt tggtaccgaa gaagaagaaa agattgcgac
    39901 tgttttgttc ggtattgcag gagtcggaga tgagcatttg caacttgtac agcaaatcgc
    39961 actttattgc agtgatatgg acaatgttgt ccagctagca gacgcgctca gcaaagaaga
    40021 agtaacggaa aatctagcaa ttgcttaagg agagagaaaa atgagagcag tacattttgg
    40081 tgcaggaaat atcggtcgag gatttattgg agaggttttg gcagctaatg gatttgagat
    40141 tactttcgta gatgtgaatc acacgatcat tgatgcttta aatgaaagaa aagaatacga
    40201 aatcgaatta gcagatgaat caaaagaaaa aatcactgta aaaaatgtca aaggaatcaa
    40261 caatcaaaca aatccagaaa aagtaatcca agaaatcgct gaagcagatt tagttactac
    40321 cgccattggc ccgaatatct taccttttat tgctgagttg attgcaaaag gtatccaaga
    40381 aagagaaaag gaaggaagca caacatcttt agatatcatt gcctgtgaaa atatgattgg
    40441 cggttcagca tttctagaaa aagaagtgta taaatatctt ccggaaacat catttactga
    40501 caaatatatc ggctttccaa atgcagcagt cgatcgaatc gtcccattac aacaccatga
    40561 agatccttta ttcgtccaag tggaaccttt caaagaatgg gtcattgacg atagccaaag
    40621 aaaaaataaa aaaatccaat taaaaggggt tttatatgta gatgatctag aaccatatat
    40681 cgaacgcaaa ctatttagtg tcaatactgg tcatgcgact gtcgcttata caggtgcctt
    40741 gttaggttac cagaccattg atgaagcaat gcaagatgcg ttagttgtgg cacaattaaa
    40801 atcagttttg caggaaacag gaagtttgct gattgccaaa tgggggtttg atgcagagca
    40861 gcatcatgcg tatattgaaa aaatcattca tcgcttccaa aacaaaaata tctctgatgc
    40921 gatcacacgt gtcgctcgga caccattacg taagttaggt tatcaagaaa gatttacacg
    40981 tccagtgaga gaactacaag aacataactt gacttgccca catttgacgg caacaatggg
    41041 gattattttt aattactatg atcctgaaga tgaacaaagc cgtcaattgc atgaaatgaa
    41101 gatccatgaa aacttggaac aattgattca agaagtcaca ggaatcaatg atccgaaaac
    41161 aatcgggaat atcaagcaaa acgtgaaccg ttatgccaag caagtcgctt aaaataggat
    41221 acgaaaaagg ggaatgtgac gaaatttttg tcacattccc ctttttcgaa taactaggct
    41281 gtttgtcctt caagataggg cagatccaat tctttaccgt aacgttgttc taaagctagg
    41341 cggatcaaac gagctgccag gttctcattt cttgcaagga taggtccatg gaaataagaa
    41401 ccgaagacat ttcggtaaat gacaccttca gattggtctt caccattatt tccttgacct
    41461 tgtaccactt tccccaaagg tcgttcacct tcacctaaga aagtccgacc attgtggttt
    41521 tcaaatccat aataagtttc attgaattct tcgttatgga taacgatatc accgataaac
    41581 cggttatttt cctgacttaa cgtgtagtga tccagtgcac tgattccttg gatcttttct
    41641 ccattagctc ccatgtagta gtgacctagc aattggtagc caccacaaat ggctaacatg
    41701 acgccatcat tttcgatata gttgatcaaa gaatctttct ttttctggat gtcttttgaa
    41761 atgattaatt gctcaaagtc ttgaccaccg ccaaagaaaa ccaagtcata tttatcagca
    41821 tcaaaaggtt catgtatact aacgatctcc gtatggcaag aaacccccat tttttctgct
    41881 atatatttca acatcaaaag gtttccgtta tcaccatatg tgttcatcaa atttccataa
    41941 aggtgggcga tattgagttc ataattagcc acggttcatt cctcctttga taaatccttg
    42001 agcagctagt tctttacgaa gctgcaatac agcagtgtaa gtagctaaaa tatagacatg
    42061 ctcagttggc aattctttga ttttttcgat tacttctggt aattcttttg tttctgtcaa
    42121 tttattttct ggaatcccgg caacttttag acgtaaagtc atatctgtat gacggtctcc
    42181 tccagaaata acagctggga tatccatatc ggcaaagttt tcaaaattac catcccaaat
    42241 ccagctgaca tcgattccgt ctgcgtaatt ggcatttaat aaagccacta aagaaaatgg
    42301 ataaggagct agaccaatca tatcgattac ttgattgata cctacaggat ttttgacaag
    42361 tactaatgta cattttttcc cattgatatt gatgacttct tgtcggccga atactttctc
    42421 gtcatatcct aaaccttgtt tgattttatc aggatcgaca ttgaagtact ctgccactgc
    42481 agtagctgct aaagcattgt agacattata cataccgcca acttcgattc cgtattcatg
    42541 accatcgatc acgaaatctg ctgacacatt gtccatccgt accatatcag ttaatcgata
    42601 atccaattca ggacgtttga agtcacaatt tggacagtaa tatttcccta agtttgcata
    42661 agtgatcatt ttataatgca agatgtgatg acaatttgga cataagacac cgtctgtatt
    42721 gtaatgagcc atttgttcac gatcttcttc atggtcaaag ccataatatt ttctaggatt
    42781 gactgtttcg atagaattga agattggtga atcaccgtta ctgataatcg ttgcatttgg
    42841 tgactttgcg gcaccgtcta caatcatttt ataggtcgta tagatttctc cataccgatc
    42901 catttgatca cggaaaatat ttgtaaataa gaacagttct ggtttcatat attctgtgac
    42961 tttgctcaaa cttgcttcat ctatttctag gacagcaaac tttttctgtc ctttttttgg
    43021 ttttgcctgc aagaaagtcg atacaattcc ctgtaccata ttcgcaccag tagggttggt
    43081 tagaacttca tcgaattgct ggcgcaaaat attgactgtt aaagcggtag tcaatgtttt
    43141 accgtttgtt ccggtgacaa cgacgacttg gtagtctttt gccaaactat ctagtatatg
    43201 tggatcgact ttcagcgcta gtttacctgg taaacttgat ccgcctttaa aaaaagtttt
    43261 taatgcccat tgcgacgttt ttccaactgc aatcgcaaaa tgactgcgta ttcccattat
    43321 gtaacctcca aaaacgattt gctgacgaat aaattcaact aatcatacca tatgttgaaa
    43381 aaaaggttaa attgcttttc ttattcttaa gaaaaggtta cggctgaaag tgcatgaaat
    43441 cggacaaaat cgcatgattt aacaaaaaaa cagcttgaaa caaaatgatt gtttcaagct
    43501 gctgtgattt tattattccc tcttacctat attcccattt taaccgataa taattttctc
    43561 gcttggatat tcatagccta gatctcgtgt ttcttttggc acgaataaca tcaatgtata
    43621 tagcattccg atccgcccaa taaacatcag tgtagcaatc gtgattttgc ccacaacaga
    43681 caagtctccg gtaatcccta aagataaacc agttgttcca aacgcagaag ttacttcgat
    43741 gatgatcgag atcagcgttt gttcttctgt agcagataaa aaaacgatac aaaagaaaca
    43801 cataccaaga gaaagcataa agacaacgac tgattttcgc acatcatctt gatcgattct
    43861 ccgtccaaaa atattgatgt tatcttcact tttgaggaag gaatatagat acaaaccgat
    43921 gattgctaca gtagttgttc gaatcccgcc gcctacagaa ctaggactgc atccgataaa
    43981 catcagtaaa gaaaaaatga tcagtgttgt gatttgaaaa tcacccaaat cgtgaatctg
    44041 cagaccagca ttacgagtcg taatcgaata aaacatcgaa ttgatccatt tgacactcat
    44101 attggaatct tggaataaat ggtccttttc caatagataa atcaagacag ttcccgaaac
    44161 aaacaagata acaaaagcta acacagctaa tttggtaaac aaggaaaaac gaaacggaag
    44221 cttcgcattg gaacgtttgt ataatagcca ttcccggcac tccatcaata caggaaaacc
    44281 aattcctccg acaaaaatca aaaacatgat caggatcaag aaaaaatagt catgtgcaaa
    44341 gggcttgatg gaatccccag tgacgtcaaa cccagagttc gtgacagccg atatcgcctg
    44401 ataaaaaccg taaaaaacgg catcacgcca acgatcaaaa tagcctctgt aataaaaata
    44461 aatagaaaag aatgtcccaa aaagtaattg aaaccagatt aaaatagcaa aagtgatccg
    44521 aatcagtcgg acgataccag acaaacgcgg ttgattcata tccgtcatga tcagctgtcg
    44581 ctgtttcagt gtgatccgtc gtttagaaaa aatgacaaaa gcagtagaaa tcatcatgat
    44641 ccccaaacca ccaacttgaa ataatatttc cagcagtata ataccgttat cattgaacac
    44701 agaattgata tcaaaagtcg atagtccagt cacactcact gtactgattg ccatgaacag
    44761 catgtccaca aaagaaacat ggctatctgg ttcgcgaaaa atcggcatat aaaatagaac
    44821 gagtgataag accgtcatca atatataata aaaaacgatg atttggatcg atgagaaatg
    44881 attggcagca aacttctgtc ctcgccgctg cagccttcga aagaataaat caacatgcac
    44941 agtactatcc tcctgacaat agtcgtttct accagtatac aaggggaatt aggaatactc
    45001 aaagaaatta tcaaacagat aacaatacag ctttcgatta aaatagaaat ggcttataat
    45061 aaggaaaatc ctagaaaaaa gaaggaggag gaataacaaa ttgattaatg ctcaacaaat
    45121 cgtagaaaaa atgaaaaatc aaaaaataaa ttatgataca gttctgaaaa aaatgatcat
    45181 gcaatgggag aaagaagaag taaagcctaa gatattaata catacatgct gtgctccttg
    45241 cagtacgcac acattggaat tcatgtctca atatgcagag atcacgttat ttttttcaaa
    45301 ttcgaatatt catcctaaaa gtgagtattt gcgaagatta catgaacaaa aacgatttgt
    45361 agaccaattt aatgagaaaa ctgaccatca tgttgcgcta atcgttgatg aataccgtcc
    45421 aaatgaattc accaaaatgg ttttaacaaa ccatcttgaa aacgaaaaag aaggtggtgc
    45481 tcgctgcagt gcatgcttca atatgcgatt agatcttgtt gcgcaaaaag cgcaagaact
    45541 aggatacgat tactttggaa gtgccctgac catctcgcca aagaaaaatg cagctttgat
    45601 caatcagatc ggaatggata tccaaaagat ttacgggaca caatacttgc caagtgattt
    45661 caagaaaaac aaaggctatg aacgctcttt gcaaatgtgc aaagagtatg atatttatcg
    45721 ccaatgttat tgtggttgcg tgttcgctgc caagcagcaa ggatgcgatc tgaaagaaat
    45781 caatcgggaa gcaaaagctt acttaaaaac atcaaccgta gtttttgagg aaaattaaag
    45841 aaatctttcg gaaaaacgta agaaaaaaat aaagaattta tggtagaaat aaggtagact
    45901 gtataatttg tccgtagtaa agtaaggaaa caagaataaa tttcaatgaa ggaagagata
    45961 agatgcgaaa agtagcacct attttgaagc caatcgctgt attaacaatc tcgacgattg
    46021 tcgcgactac tacaggtatt gtgaatagtt ccagctaccg tgcaccaagt tcaagttcta
    46081 gtactgtaga agaaacaaca ccaactactt cagtctcgac atctgaacca acaacaacgg
    46141 aaagtttgaa tgaaaccact acgacaagta catgggacga tacaacaact agttcgtcgt
    46201 atgatgagac atatacaact gacagctcaa gcgaaacaag taatacggta ccagatggca
    46261 ctccatcgga ttcaaccaat ccaagtacaa gtccggatac aagtgctagc tccgaaacga
    46321 taccaagcag tacgactgta gatgataaca cgcaaaatga tgatgcagca cgtacagcac
    46381 agcaaaacca agattcagga aatacccaaa cgtataacca gcaagcaggt gattctcaat
    46441 gacattttta ctagacctta tcaacggggt gcataaattc cttggctatt tagacatcag
    46501 tcctaaatat cttaaccgtg gatatacgat tttaagtgtg attcccacgc tttatttatt
    46561 gagaattgtt tatggacttt ggcaaaacca aaactatctt caatttttct tatatggact
    46621 tgcatttcta gtgcttgttt attttactgt tttaaatgta ttttattact ttttagataa
    46681 aaacacgaaa gccgatgtca ctcagctttt tgtgaagtat ttacctgatg aagcgttcaa
    46741 catccagaca gaagaatcga tagataatgg aaattcgatc gataaagtga atacacaaga
    46801 ggtcatggtt tcttatgacg aagattatca attgaaatta gccgagaata tgcggtattt
    46861 gattggaaat ggtgagatca aaacaaatga actcggtcgc atggatggat ttctggtcga
    46921 tagaaataca ttgtatcctt attactatgt caagaaaaca ggagagaaag agtatgttct
    46981 aaatattgga cgaagctact cggacatgga aaaagtaggg accattcgac ttgactcaac
    47041 agaagacaca ttggaacctg tcggattgtt catcgtcggc ggtgattttg tcaaagacgg
    47101 gttacgctat catgagcctt atcgtctgaa attgattgca aaaaaggcac agtcgcagga
    47161 agaaccagtg gtttatagtc gtagccaaag aagaagacat tcataatgtg atgaaacgcc
    47221 gtgtatcagt ggttgcgata aaaacgttta ttcggaataa gggggatgtg acacgtcttt
    47281 tgtcacgttc cttttttata taacgagaaa gataagacga acacatcact tattctttcc
    47341 tttcatttat aaacattgta gaataaagaa agtacaacga agtattgtga aaaaacgtaa
    47401 taaataacga ggaaaataca agtaggaggt aagctgtgaa agaatttttg aaaaattggg
    47461 ggattttgat ccttgtatta gcggctattt tattagctag agtgtacgtg tttacaccag
    47521 ttactgtaaa cgggcattcg atggacccta cattaagtga tggacaacga ttgatttcat
    47581 ctaagatctc aaactatgaa cgaatggata ttattactac aaaggaaccg ggggatgaag
    47641 aacggatgat tgttaaacgg atcatcggta tgcctggaga tacagtcaag atggaaaatg
    47701 accagttgac cattaatggc aaaaagtatg atgaacctta tctggatgag ttcaaaaaag
    47761 aattttcaga agataagtta caaggagaat atgcttatag ctcaggattc caagcacaag
    47821 cagaaagttc atccaccttt acgagtgact ttgaatatac tgtgccaaaa ggaaagtatc
    47881 ttgttctagg cgataaccgc ttgatttcaa aagacagccg aatgtttggg ttagtcgaca
    47941 aagatatgat ccaaggaaaa gttgttttcc gttattggcc tctttcagaa attaaaatca
    48001 tgtgatgaaa aaataaaaag aagctgtgac atgttttttg tcacagcttc tttttccgaa
    48061 aattccttct tatttcttgg agttgctcac tttttttgca atctcatttt aagctttttg
    48121 gctgatggat aagaaaagcg aagcgttttt gttcataaat agcttttccc cacgatggaa
    48181 ggtatgacta gtagcagaga caaagcccgt tttttccaat cgattattca attcttcttg
    48241 gctaaagcca ttatggactt tcggatgact aatttgttca ttcttgtcaa agtctacgat
    48301 gatcaattga ccgccaggag ctaaaatttc ataaagtttt gtaagaatat tctccgtatc
    48361 aggaatgtgt aaaagtacaa gtgatagaag gatgagatta gcttggacag caggaaattc
    48421 gacagaagca tcagcgtgga tcgttcggac atttttcaga tctgctgctt ggatcttttc
    48481 ttctgccatt ttcagcatag tttctgaagc atcggcaatg atcagttctt tgaatctttc
    48541 cgcaagcggt aaactaacaa gcccagttcc gcctccgtaa tccagcagga ctttttggtg
    48601 gctgtcgcgc ggtagataag tagttagttc ttcgcgaata atcttggcta atgcttgacg
    48661 gtcaggggaa tcataatggt tagcgatgga atcaaaaata ttttttgtca tcaaatagct
    48721 ccttacgtaa aactttgaaa ttttaactta ttatagcacg tgaaaagaaa gaaagcatgc
    48781 tatactcaat tcaaaaaggg agaatctaga aagaatatgc cgatatcgtt tgtaaaagat
    48841 agagaggaaa aaggaaagtg tgtccgagaa atattactag acttgcctga gtggtttggg
    48901 cttcctgaat caacggaaaa atatatcgag gaatcaagta aactaccttt atggtgtgaa
    48961 aaaagaaaag aagaatattt aggctttatc actctttcac agacaagtga agatactgcc
    49021 gaaatctatt ctattgtatg ggaataaaaa aagactatca tcaccaagga ttaggaacaa
    49081 aactttttaa agaagcagaa ggttacgcag caaaacatta caaatatttg caagtgaaga
    49141 ctgtagacga ggggcactac tctatatatg atcaaactat ttgtttctac gaatcattag
    49201 gattttcaag gttggaagtt tttccaaatt tatgggatga atggaaccca tgtttagttc
    49261 ttgtcaaaaa attggaacaa aagtgagcaa tagatttaat actaatctgt gcttgatgaa
    49321 aattctcact gtaaaaaatg ctgaaaatca agtataatac cataggaaca aactggaagg
    49381 tgaaaaagag catggcacaa ctatttttta agtatggcgc aatgaatagc ggcaagacga
    49441 tcgagatcct aaaagtcgca cataattatg aagaacaaaa taagccagtt gtcttaatga
    49501 caagcggtat tgatacaaga gatggtttag gcgttgtttc aagccgaatc ggactgaaac
    49561 gtaatgcgat gccgatattt gaagaaacag acgtatttga cgtaattcag aaaatggaaa
    49621 gcaaacccta ttgtgtgctg atcgatgaag cgcaattctt aaaaaagaaa catgtcattg
    49681 cttttacgag aattgtggac gaattggata ttccggttat ggcatttggt ttgaaaaatg
    49741 attttcgaaa cgagttattt gaaggatcaa aatatttatt gctttatgca gacaaaatcg
    49801 aagaaatgaa aacaatctgt tggttctgcc acaaaaaagc aatcatgaat ttacattata
    49861 ttgatggaaa ccctgtttat gaaggcgatc aagtgcaaat tggcggaaat gaagcttatt
    49921 accccgtttg tcgaaaacat tactttcacc catcaatgat tacagaagga gaataagcat
    49981 gtacgatcaa ttacaagcaa ttgaagaccg ttatgaagag cttggagaac ttttaagtga
    50041 cccagaagtg atttctgata caaagcgttt tatgcagctt tcaaaagaag aagcaaacac
    50101 gcgcgaaaca gtcgaagttt atcgtcgtta taaagaagta gtacaaggaa tcaccgatac
    50161 agaagaactg cttggtgaaa aactggatga tgaaatggct gaactagcaa aagaagaatt
    50221 atctgaatta aaaaaagaaa aagaagtctt agaagaaaaa atcaagatct tgcttttacc
    50281 aaaagatcca aatgacgata aaaatatcat catggaaatc cgtggagcag caggcggaga
    50341 cgaagcagca ttattcgctg gcgatctttt caatatgtac caaaagtatg ctgaagcaca
    50401 aggctggaaa acagaagtga tggaagctaa cgttaccgga atcggtggat ataaagaagt
    50461 gatcatgatg atcaccggag acaacgtata ttcgaaatta aaatatgaaa gtggtgcgca
    50521 ccgcgtccag cgtgttcctt caactgaatc gcaagggcgt atccatactt ccaccgcaac
    50581 agttgtcgtg atgccagaag ctgaagaagt agagatcgat attgaagaca aagatatccg
    50641 tacagatatc tatcatgctt caggtgctgg cggacagcac gtcaataaaa cagcatctgc
    50701 tgtacgtttg actcacttac caacagggat cgttgttgct atgcaagatg aacgttctca
    50761 aatcaagaac cgtgaaaaag caatgaaaat cttgcgtgct cgtgtgtacg ataagatcca
    50821 gcaagaagca caaagcgagt atgatgccaa tcgaaaatca gcagtcggaa ctggggatcg
    50881 ttcagaacgg atccggacgt ataatttccc gcaaaatcgt gtgactgatc atcgtatcgg
    50941 attaactatc caaaaattag atcagatttt agcagggaaa ttagatgaga tcgttgatgc
    51001 attgatcatg tatgatcaaa catcaaaact ggaagctatg caaaatgggt aaaatgacct
    51061 atcgagaagt cctgtcacgg gcttcttctt ttttagagga tcatgggaaa gaagcatata
    51121 gtatccaatt tttatttttg gaacgaaaac aatggaaaaa acttgattgg ctccttcata
    51181 tgaacgaaga gatttcagaa gaagaacagc ggttgatcga aaccgatctg cgacttctgc
    51241 ttgcagatca tccacctcag tatttgctag gatatgcaga tttttatgat catcggttaa
    51301 aggtaactga agcaacactg atccctcgac cagaaacaga ggagttagtc gaatggtgtt
    51361 tagacgagac gcctggtgta ccgttagaag tgatagatat tgggacaggg actggagcaa
    51421 ttgctattag tttgaaagca gcaagaaaaa attggcatat atctgctgtg gatctttcag
    51481 aagaagcatt agaagtagcg aaagaaaatg ctcagaaaga aggaacgaaa atcagttttt
    51541 atcatggaga tacattagga ccagtaatgg atcagcaatt tgatgtgatt atttctaatc
    51601 cgccgtacat cagtcggaat gaatgggaac tgatggatga aagtgtccgg tcattcgaac
    51661 ctaaaatggc cctttttgct gagaacgatg gattagctat ttatgaaaaa atagcagttg
    51721 aagcaccaag cgtactaaaa tcaaatggaa agattttttt agaaatcgga tttcgccaag
    51781 gtgaagcagt aaaaaaaata tttcagcaag catttccaga taaaaaagtg gcgataaaaa
    51841 aggatctttt tggcaatgaa cgaatgatcc aagtttatgg ttgaagttgt ggatgaaaaa
    51901 ttttttattt gtttttttat tataaaaaaa cacttatttg acaaggatgt aaacgacttt
    51961 ttggtttatc aacaagttta tccactattg acaaatagat tatccacaaa cagtggaaaa
    52021 cttgaggata aactcagaca aaataagaat gtatgaaaaa ggggttgtgg ataaactgtg
    52081 gaaacgaaac gatatgatga aacagaattg caagaggcag ccgaagcact gaaagaagga
    52141 gaacttgttg cttttcctac cgaaacagta tacggattgg gcgccaacgc tcttttacca
    52201 aacgctgtca agaaagtgtt ttctgtaaaa gggagaccgc aagataatcc attaatcgtc
    52261 catgtcgcct cttttgaaca agtaaaagag tatgtggaca actttcatcc ggcagcagag
    52321 aagattgtga agaacttctg gcctggacca ttaactttga tatttaagat caaaaaagac
    52381 actttgccct cagttgtgac gggaggatta tccacagctg catttcgtat accggataac
    52441 aaaaaaacac tagaagtaat cgaactttcc ggtgttccgc ttgtcggtcc aagtgcaaat
    52501 acgtcgggaa aaccaagtcc aacgactgca gatcatgtat atcatgatct acaaggaaaa
    52561 attaccggga ttattgatga tggagctacg cgaattggtg tagaatccac tgttcttgat
    52621 ttaagtgatc caacagccat gcctatgatt ttacgaccag gtgcagtaac gaaagaacaa
    52681 atcgaagcag tcatcgaatc tcctgttgct attgatcagc atcttgtaaa agaaaatgaa
    52741 acaccaaaag cccctgggat gaaatacaaa cactactcac ccgatacacg tgttctgatg
    52801 gtcagagatg gcgattggtc aactgcagtt caatgggcaa aaaataagaa gatccgtgca
    52861 ggtgtcatag caagccccga aattgcggat caagtgcgga cagatactgc agctgtttat
    52921 atgtataacg acaactcagt agaagcggcg gctaaaggat tgtttgcagg tctaagagga
    52981 ttagatgaac caacacttgg attagatttg atttttgtac aagtgtatcc agaaactgga
    53041 ttggggaatg cttacatgaa tcgcttgaaa aaagcagcag gacaaaatta ttttgaaaaa
    53101 tgatcaattg gttattgtcg cattctttta gtatgataca atgctatcaa gtaaatcttc
    53161 tatcagtgaa cgaaaaacta agcgtaacta atcgtaatag aagagatacg aatatttggc
    53221 aatttgtttt tgatgggaaa aataaaattg taggaaaata aaaaggagct gttgtttgtg
    53281 gtagattaca aaacgtttga ccctgatctt tgggctgcga tcgccaaaga agaagaaaga
    53341 caagaacata atttggagct gattgcctct gaaaactttg tctccgaagc agtgatggct
    53401 gcacaaggaa gtatcttgac aaataaatac gcagaaggtt atccagggca tcgctattat
    53461 ggtgggtgtg aatttgtcga tatcgttgaa aatttagcga ttgaccgtgc aaaagaatta
    53521 ttcggagcga aatttgcgaa cgtccagcct cactccggct ctcaagccaa cacggctgct
    53581 tatctcgctt tggtagaacc aggggacacg atcttaggaa tggatctttc cgcaggcgga
    53641 catctaacac atggatcacc agtcaacttc agtggcaaaa cctatcactt tgttgcttac
    53701 ggtgttgatc cgacaacaga agttattgat tataatgtgg tacgtatttt agctagaaaa
    53761 catcaaccaa aattgatcgt agcaggtgcc agtgcttacg gacgaacgat cgactttgcg
    53821 aaattcagag aaatcgcaga tgaagtaggc gcaaaactga tggtagatat ggcacatatc
    53881 gcaggattag tagcagcagg acttcatcct aatccagttc cttatgcaga tatcacaacg
    53941 actaccactc ataaaacatt gcgtggccct cgaggcggca tgattttgac aaacgatgaa
    54001 gcattagcaa aaaaaataaa tagtgcggta tttcctggta ttcaaggcgg ccctctagag
    54061 catgtgattg cgggtaaagc agtagcattc aaagaagcat tagatcctgc atttaaagaa
    54121 tatagtgaac aaatcatcgc caatgcaaaa gcaatggtca aagttttcaa tcaagcaatt
    54181 ggaaccagag tcatttcagg ggcgacagat aatcatctga tgttgatcga tgttcgcgag
    54241 ttaggtataa atggaaaaga agcagagagc attttggaca gtgtgaatat cactgtaaat
    54301 aaaaactcga ttccttttga aacactaagt ccattcaaaa caagcggtat tcgaatcggc
    54361 acgccagcaa tcactactcg cgggttcaaa gaagaagatg ctgtaaaagt ggctgaacta
    54421 gttgtcaaag cattacaagc aaaagatgat aacgctcaac tagacgaagt caaaacaggt
    54481 gtacgcgaat taactgaaaa atttccgttg cataaaaaat aaaaatttaa attccattag
    54541 taaacagaaa agttcaatgt tgggcttttc tgtttatttt ttgggaaaac ggattctttg
    54601 acaatatcgg tgatgctctc tagtatatga ggtgtaattt tagtcttcac actaaatctc
    54661 agggaggaaa agtcatgact gatcgtatct cgttagaaaa ggaagcagta gcattcagtg
    54721 atgcaaatgc gcctcatccg cgtatctatg aactagcacc ggaagaagga agagttttac
    54781 tagaagaagt acaagaatca cctgtgaaca aatattccgt cgatattgaa gacttgatgg
    54841 tggatactgg cgaatgggga aaactcaata ttcgtctcgt acgaccagaa gggaatatgg
    54901 ataaattacc agttattttt tatattcatg gagcaggctg ggtttttggc agtgctcaaa
    54961 cccatgataa attgatcaga gaacttgcgg ttcgtacgaa ttccgtcgtt gtctttccag
    55021 aatatagtcg atcaccagaa gcaaaatatc caacagcaat tgaacaaaat tatgctgtgt
    55081 tgcaacagct gaataaatta gccaagtcaa aaaatctgga tctcgagaat ctaatcgttg
    55141 caggagattc agtaggtggt aacatggcta cagtcatgac gatcttaaca aaagaacgaa
    55201 agggcctccc aatatccaag caattgctgt attatccagt cacaaacgct gattttgata
    55261 cagattccta tcatgaattc ggagaaaact attttttgac aagagaagga atgatgtggt
    55321 tttgggacca atatacgacg aatgaagaag agcgtgctga aatcacagct tcgcctttga
    55381 gagcaaatat aaatgaacta aatgatcttc cttcagcaat gattttaacg ggagaagcgg
    55441 atgtattaag agatgaaggt gaggcttatg cgcgcaaact tcgtgatgca ggggttcctg
    55501 ttacacaagt acgcttccaa ggaatcatcc atgattttgt catggtaaat gctatggatc
    55561 agacaaaggc aacgcgtgca gcaatggcat tatctacttc ttggatcaag aaaaaagaat
    55621 aagctttgct tgtccgcagg aaaacttctt ttcaaaaacg gattttctta ggaaaaatgg
    55681 cgagtttgac tagtcaatca cttgattttg ttttacaatt aaaaagaaac gacagaacaa
    55741 aggagagctt aaaaaatggg caaatttcaa gtgatcgatc atccattgat tcaacacaaa
    55801 ctaactatta ttcgggacaa aaactgcgga acaaaggttt ttcgtgaagt agtcgatgaa
    55861 atcgctatgt tgatggcata tgaagtatca cgggatatgc cattggaaga catcgtgatc
    55921 gaaacaccga tcactgaaac aacacaaaaa acgttgtctg gtaaaaaagt agcgattatt
    55981 ccaatcttgc gcgcaggtat tggaatggtt gacggtatct tggaattgat tccagctgct
    56041 aaagtgggac atatcggtct ttatcgagat gaagaaacat tggaaccaca tgaatatttc
    56101 gtcaaacttc cagaagacat tgatgcacgt cagttgtttg tagtagatcc aatgttggca
    56161 acaggcggat cagcaatcat ggcaatcgat gcattgaaag aacgtggcgc gtcgaacatt
    56221 aaatttgttt gtttggtagc agcacctgag ggagtaaaag ctttgcaaga agcgcatcct
    56281 gatattgaca tctatacagc ttctctagat gaaaaattga atgaacatgg ttatatcgtt
    56341 cctggactag gggatgcggg tgaccgtttg tttggtacaa aataagtaaa tacgaaaaag
    56401 ggtgtgacac aacttttgtc acatcctttt ttggtcattt ttaacgtttg atgcagaaca
    56461 gctttttcac agctttcgtt tgcatataaa aaaaccatcc caaaaatgca gagatggtca
    56521 aattcaaatc ttttttagtt ctttgtagtt aacgattact acacccttac tttgcgtcat
    56581 caagttcgca ttttctgcag tttcatcaat ctcaacaaca gcggaatttt tcaattgctt
    56641 tgaaatcgtt ccagtttgcg acttgccgtg aattgtgaac attacgtgag tattgatctt
    56701 gaatttttca ccaatttccg atggtacgat agttgtatca aattttccaa aagttgccat
    56761 tttcataaaa cctgcctttc acatcaatta taccacgttt tatgaaaaat atcatgaaat
    56821 taaactaatt ttccatcgga ctctttacaa tcagccaaat agagtatata atttcttttg
    56881 gataaggcca gcttagctca gttggtagag caacgcactc gtaacgcgta ggtcgtaggt
    56941 tcgatcccta tagctggcat taaaagagaa gacagatata tcaaggattt ttaatccatt
    57001 gatacatctg tctttttgtt taaaggaaat aaagtatgct ttcttagcaa tagagcatgt
    57061 attatttcta taaaaattta ggagaataaa acgactagca atatagctgt gaaatcaaat
    57121 atcacatgaa ctgcccaact tgtaatgatc gagttgcttt taatggcagc ttggttgaat
    57181 aacaatcttg ccaccccttg tatcaaaagg atgtgtgcta cagtgtgtaa aatatttcca
    57241 ttatcataag tactgtaatg gataaatcca aagatgattg ctgacaagaa actagcgaca
    57301 ggaatgctga acttactaga aaaaatagcg agaaaataca ctgagaacaa ttcttctcca
    57361 agtatcatga taggcaaaat aataaataat aacgggctga aagcctcatt tattggattg
    57421 cctgttaaac cccatttcaa aatttgttgc aaaaataccg agactgcggc actgattact
    57481 acattgataa aaaaatattt ggctatattt ttgacaggag cgacaggttt cgagaacatt
    57541 tttttcgtat cttttttacc aaaaacaagt gtaaatgcta taaatccagt gatagttaaa
    57601 gaaaaaatgc taataatgga tggcgcaaat agcaacccta acaaaccgag tacaacgaca
    57661 atagtgccca atatcccttt tttaagttct attttttttc ctaaaatgtt attcatgata
    57721 ataactccta ctattcagtt tttgaagata gagtcagtat acatgaagag ttaaaatagg
    57781 caatgttttt ttatggatat ttgtat
//
LOCUS       DACCXO010000007        53567 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.007, whole genome shotgun sequence.
ACCESSION   DACCXO010000007 DACCXO010000000
VERSION     DACCXO010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.007"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            169..2334
                     /locus_tag="IVY70_000372"
     CDS             169..2334
                     /locus_tag="IVY70_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7957533.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            2638..3471
                     /locus_tag="IVY70_000373"
     CDS             2638..3471
                     /locus_tag="IVY70_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP7957534.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(3573..5273)
                     /locus_tag="IVY70_000374"
     CDS             complement(3573..5273)
                     /locus_tag="IVY70_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957535.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            5780..6940
                     /locus_tag="IVY70_000375"
     CDS             5780..6940
                     /locus_tag="IVY70_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7957536.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6956..7399
                     /locus_tag="IVY70_000376"
     CDS             6956..7399
                     /locus_tag="IVY70_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7957537.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(7882..8412)
                     /locus_tag="IVY70_000377"
     CDS             complement(7882..8412)
                     /locus_tag="IVY70_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP7957538.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            8517..9068
                     /locus_tag="IVY70_000378"
     CDS             8517..9068
                     /locus_tag="IVY70_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7957539.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(9221..9694)
                     /locus_tag="IVY70_000379"
     CDS             complement(9221..9694)
                     /locus_tag="IVY70_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7957540.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            9832..11304
                     /locus_tag="IVY70_000380"
     CDS             9832..11304
                     /locus_tag="IVY70_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7957541.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            11301..12011
                     /locus_tag="IVY70_000381"
     CDS             11301..12011
                     /locus_tag="IVY70_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP7957542.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            12363..12647
                     /locus_tag="IVY70_000382"
     CDS             12363..12647
                     /locus_tag="IVY70_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP7957543.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(12928..13347)
                     /locus_tag="IVY70_000383"
     CDS             complement(12928..13347)
                     /locus_tag="IVY70_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP7957544.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(13349..13933)
                     /locus_tag="IVY70_000384"
     CDS             complement(13349..13933)
                     /locus_tag="IVY70_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP7957545.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(13980..15455)
                     /locus_tag="IVY70_000385"
     CDS             complement(13980..15455)
                     /locus_tag="IVY70_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP7957546.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            15636..15947
                     /locus_tag="IVY70_000386"
     CDS             15636..15947
                     /locus_tag="IVY70_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957547.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(16062..18398)
                     /locus_tag="IVY70_000387"
     CDS             complement(16062..18398)
                     /locus_tag="IVY70_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7957548.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            18540..19208
                     /locus_tag="IVY70_000388"
     CDS             18540..19208
                     /locus_tag="IVY70_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7957549.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERVGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            19211..20254
                     /locus_tag="IVY70_000389"
     CDS             19211..20254
                     /locus_tag="IVY70_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7957550.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(20491..21372)
                     /locus_tag="IVY70_000390"
     CDS             complement(20491..21372)
                     /locus_tag="IVY70_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7957551.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            21531..22259
                     /locus_tag="IVY70_000391"
     CDS             21531..22259
                     /locus_tag="IVY70_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP7957552.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            22415..22732
                     /locus_tag="IVY70_000392"
     CDS             22415..22732
                     /locus_tag="IVY70_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7957553.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            22725..23333
                     /locus_tag="IVY70_000393"
     CDS             22725..23333
                     /locus_tag="IVY70_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP7957554.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            23349..24179
                     /locus_tag="IVY70_000394"
     CDS             23349..24179
                     /locus_tag="IVY70_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP7957555.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            24317..24805
                     /locus_tag="IVY70_000395"
     CDS             24317..24805
                     /locus_tag="IVY70_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP7957556.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            24856..25728
                     /locus_tag="IVY70_000396"
     CDS             24856..25728
                     /locus_tag="IVY70_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP7957557.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            25904..26266
                     /locus_tag="IVY70_000397"
     CDS             25904..26266
                     /locus_tag="IVY70_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7957558.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            26275..27027
                     /locus_tag="IVY70_000398"
     CDS             26275..27027
                     /locus_tag="IVY70_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7957559.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            27041..28843
                     /locus_tag="IVY70_000399"
     CDS             27041..28843
                     /locus_tag="IVY70_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7957560.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            29120..30160
                     /locus_tag="IVY70_000400"
     CDS             29120..30160
                     /locus_tag="IVY70_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP7957561.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(30212..32356)
                     /locus_tag="IVY70_000401"
     CDS             complement(30212..32356)
                     /locus_tag="IVY70_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7957562.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(32713..34086)
                     /locus_tag="IVY70_000402"
     CDS             complement(32713..34086)
                     /locus_tag="IVY70_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7957563.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(34086..34481)
                     /gene="rbsD"
                     /locus_tag="IVY70_000403"
     CDS             complement(34086..34481)
                     /gene="rbsD"
                     /locus_tag="IVY70_000403"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP7957564.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(34509..35426)
                     /gene="rbsK"
                     /locus_tag="IVY70_000404"
     CDS             complement(34509..35426)
                     /gene="rbsK"
                     /locus_tag="IVY70_000404"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP7957565.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(35534..36547)
                     /locus_tag="IVY70_000405"
     CDS             complement(35534..36547)
                     /locus_tag="IVY70_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7957566.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(36628..37227)
                     /locus_tag="IVY70_000406"
     CDS             complement(36628..37227)
                     /locus_tag="IVY70_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7957567.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            37413..38114
                     /locus_tag="IVY70_000407"
     CDS             37413..38114
                     /locus_tag="IVY70_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7957568.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            38124..40835
                     /locus_tag="IVY70_000408"
     CDS             38124..40835
                     /locus_tag="IVY70_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP7957569.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            41286..42914
                     /gene="treC"
                     /locus_tag="IVY70_000409"
     CDS             41286..42914
                     /gene="treC"
                     /locus_tag="IVY70_000409"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP7957570.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(42974..44155)
                     /locus_tag="IVY70_000410"
     CDS             complement(42974..44155)
                     /locus_tag="IVY70_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7957571.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            44407..45930
                     /locus_tag="IVY70_000411"
     CDS             44407..45930
                     /locus_tag="IVY70_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP7957572.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(45999..46727)
                     /locus_tag="IVY70_000412"
     CDS             complement(45999..46727)
                     /locus_tag="IVY70_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7957573.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(46740..48020)
                     /locus_tag="IVY70_000413"
     CDS             complement(46740..48020)
                     /locus_tag="IVY70_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7957574.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(48161..48763)
                     /locus_tag="IVY70_000414"
     CDS             complement(48161..48763)
                     /locus_tag="IVY70_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP7957575.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            49008..49214
                     /locus_tag="IVY70_000415"
     CDS             49008..49214
                     /locus_tag="IVY70_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957576.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            49526..50245
                     /locus_tag="IVY70_000416"
     CDS             49526..50245
                     /locus_tag="IVY70_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP7957577.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(50326..51009)
                     /locus_tag="IVY70_000417"
     CDS             complement(50326..51009)
                     /locus_tag="IVY70_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP7957578.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            51227..53413
                     /locus_tag="IVY70_000418"
     CDS             51227..53413
                     /locus_tag="IVY70_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP7957579.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        17431 a         9414 c        10811 g        15911 t
ORIGIN      
        1 aagcttaaaa aaataaattt ttaggagaaa attatagaag ggaacacacg tattataggt
       61 tttatagaat ggtatagaca gaaaaataaa aaataaagca tttacaaaaa agaaaacgca
      121 cgctataata cgtttaacga aaccgattgc gtaaaggagc taaacggaat gaaaaagata
      181 tttagttttg agttttggca aaaatttgga aaggctttaa tggttgtggt agcagttatg
      241 ccggcagctg ggttgatgat cagtataggg aaatcaattc ccttgatcaa tcctgattgg
      301 acgccgcttg tcacaacggg cggagtaatt gaaaatattg gttgggcaat tattggtaat
      361 cttcatattt tatttgcttt agcaatagga ggaagttggg caaaagaaag agcaggcgga
      421 gcatttgcag ctgggatctc atttatttta atcaatcgga tcactggggc tatctttggt
      481 gtcacaagtg acatgcttgc gaatgaagat gcatttaccc acactctgtt cggtacgaag
      541 atcatggtta aaggattttt tacaagtgta ttggaagcac cagctttgaa tatgggagta
      601 ttcgtaggga tcatcgctgg atttgtcggg gcaatggctt ataataagta ctataattat
      661 agaaaattgc cggatgcttt gtcatttttc aatggaaagc gctttgttcc gtttgttgtt
      721 atcttatggt caacaatcgt ggcattgatt cttgctgtgg tgtggccaaa cgtacaggct
      781 ggaatcaata attttggttt atggattgca gaatcacaag aaagcgcacc gattctagca
      841 ccgtttttat ttggaacatt agaacgttta ctattgccat ttggattgca tcatatgctg
      901 acgattccta tcaactatac acagcttggc ggatcgtatg agattttatc aggcgcacag
      961 gcggggacac aagtatttgg gcaagatccg ttatggttgg cttgggccac tgatttagtg
     1021 aatcttaaag gtgcagggga tatgtcgcaa tatgagtttg tgttgcataa ctggacgccg
     1081 gctcgtttca aagtaggaca aatgattggt tcttcaggta tcttgatggg aatggctttt
     1141 gcgatgtatc gtaatgtcga tcctgataag aaagcaagat ataaatcaat gtatttttca
     1201 gcagcgttgg ctgtctttct aacaggtgta acagaaccat tggagttcat gtttatgttt
     1261 gctgctgtgc cattatatgt catttattca gtgatccaag gggctgcatt tgcgatggca
     1321 gatatcttgc cattgcgggt ccattcattt gggaatatcg aattgctgac tcggacaccg
     1381 ttagctatca aagcgggtct aggtggagat cttatcaact tcgtgttgtg tgtgatcgct
     1441 tttggtgttg ttacgtattt cttagcaaat ttcttgatca aaaaattcaa ctttgctaca
     1501 ccgggacgta acggaaatta cgacaatgac agtgaagaaa ccgttgtatc taacagcgga
     1561 acaggaacag cagatcagca agtggtccag atcattcatt tgctgggagg aaaagaaaat
     1621 atcaaagatg tcgatgcttg tatgactcgg ttacgcgtaa gtgtaaatga tcgagaaaaa
     1681 gtagggactg aagaagcctg gaaacgagca ggggcgatgg gattgatcgt caaagacaat
     1741 ggagtccaag cagtttatgg accaaaagca gatgtcttga aatcagatat cgaagatctg
     1801 ctgcaatcag gagcagagat tccagagcca gaaatggttt ctacaggaaa tggacaagca
     1861 ggtagtaagc aatacttagg tatagaacaa gaactagttt ccgcagcttc tggagaagtg
     1921 attcctttat ctgaagtaaa agatccggta ttctcacaaa aaatgatggg tgacggcttt
     1981 gctgttattc caacatcaag agaagttgtt gcgccgatcg ctggaaaagt gacaagtatt
     2041 ttccctagca aacatgcaat tgggctggaa acgaaagatg gtatcgaagt tttgatccat
     2101 atgggaatcg atacagtcca aatgaatcag ccagcttttg agattgctgt caaagaaggc
     2161 caagaagtag gaacaggaac gaaattagct gagatggatc ttgatgtgat ccaaaacgaa
     2221 gaaaaagatc caacgatcat gatcgtattt acaaacgata aagtagaaga agtagtgatc
     2281 aagcaattag gtacagtgac agctggtaaa gtgatcggct ccattaaaat gtaaggagag
     2341 gttgtgaaaa agctgtcctg catcaagtca taagaacagc caaacaaaaa tagctcctcc
     2401 tatttttcgt ttggttgggc ttattaccga gaagcagcag tttgaatact gtttagtgga
     2461 gaggtcgtga aaaagctgcc ctgcaccaag tgataaaaac agccaaacaa aaaatagctc
     2521 ctggtccatt gagcttatta ctgaggagtt agcaggagaa cactatttgc tggagaggtt
     2581 gtgacaaaaa ttttgtcaca acccttttgt ttcgaaatga agacaaggag aaaaccaatg
     2641 aaattcatga cactcaacac gcatagctgg cttgagccag aaccagagaa aaaattgcaa
     2701 gaattagcag ataagatcct gttagaagat tatgaagtga ttgctttaca agaaatcaac
     2761 caattactgg aatctgaaga agtagaacct gcgaagctaa tgaagttctg tccggtaaag
     2821 aatcaagtac ctatccgtaa agataatttt gcctaccgtt tagttcagct tttaaaggaa
     2881 cacggaaaag agtatttttg gagttgggaa atgagccaca tcggttatga taaatatgaa
     2941 gaaggaaatg cactgcttac gaaaaaaaca ttggaaagtc aggtccttac cgtctcccaa
     3001 tttcaaaaga aagaagatta tcagacacgc aagatcttga ttgggaaaac aaagatagat
     3061 gatcaggaca tttttgtctc gagctgccac ttttcttggt ggacagataa aaaaagtggc
     3121 ttttattttg agtggaaaaa cttggaaaac tactttttgg aaactcgcgt accacttttt
     3181 ttcctaggtg atttcaataa tccagtagac tctcaaggtt attatactgt aagagaaagt
     3241 tgccttcttc ttcaagattc gtatgttgtc gcgaatgaaa aaggaaaagc agctactgtg
     3301 gaaaagaaaa tagatggttg ggaacaaaat acagaaaaat tgcgtataga ttttattttc
     3361 gtccctgaag gaatgcaggt taaaaaatat caacggattt ttgatgggat cgatagtcca
     3421 atcatcagtg atcactacgg ggtggaaatc gaattagatg tgaacgaata aaaaatataa
     3481 catataacat aaaaaaactg attaaaataa aactctccaa acgattatac cgtttggaga
     3541 gttttttgat cggatgtatt tatccctttt gcctaagcac gtaaaagctt gtatctatga
     3601 taaataaata aacatagcaa ggtaaaccag ccaatcaaag taagagaaag aatgaaatag
     3661 aaatagattg gcgtttgata atgaagcgtg agctcgttga ttcctttttg ctgcttcacg
     3721 attggggtcc cgatatctgt tacttcataa tcagtcaatg tttttccatt ttgctgtagc
     3781 acagtccgat cgtagacgat aacagggata tgaacagttt tgtccccagt gtttttccac
     3841 ttgattgtca aaagaccgcc tggatgtgtt tttataaaag ttgtttcgtt ttctagtatt
     3901 tcttcgttat accggtcgta tttattgtct tttgtctcct tatagattgg cagatagtca
     3961 ggtgtggttt tttggaaaac gaacaagctg gtagacatat ctttgctgaa gaatgttttc
     4021 cttgcttctt ccggcgtatc gaatagataa gtatgtcgac tgacaaatgt ttcattttcc
     4081 cacttttcca gatgctggta taagttctgg atcgtctgtg ctgaaaaaat aagaatgcca
     4141 aatatactta aaagacggaa ccacttttgt tcagaccact ggttcagtga aagggcaagg
     4201 taaagtagaa gcaatagcgt aaatggcacg aaaaatcgaa atggaaactg gatcaaacta
     4261 ataaaaggca catttttttc gatcaatgtc gtccatggaa tcaagctagt agaaagaaaa
     4321 aagaaaacaa gacttgtgta caacacgatt ttttggaata aggtgttctt ttgttgaaag
     4381 aaaataaaaa gcaaaaaacc tgataatatc aatataggaa aggccttagg gtaatggagc
     4441 caataactgc catccagatc tatcgccttt tgactcattt ctctgttaac aaagggagga
     4501 agaatcttat tcccactgta aacaatagct aatcctgccc aaatatttgc tgtcagaaca
     4561 aaataaagca agaccgactt aatcagttga agcagtgctt ttcctttttc agtctggtta
     4621 aaaaagtaat aaagataaaa agggatatac atcaaagcta acatgatcga agacagtaaa
     4681 tggatctgtg tcataagtcc cactgagaca gcgactttga taacagggaa ctttttatga
     4741 aggacgaaat cagcaatagg gatcatacat aaaggataaa aacaagtccc ccagctagtg
     4801 aatccttggt tgatagacca gtattgaata gaatacgtac tcatgaataa aacggctact
     4861 ggtacactta cccaatttcg aacattgaca tatctcagta aaatcagcat agaaaatcct
     4921 gaaataagat aaagaatgaa atttgaaaga atctgataat gaaaccagct acctgcaagc
     4981 aacacgatca atccttgtaa ataagcgaaa aatgggccgt aaacggcatt cacgatcctg
     5041 ccactttgct ggaatccata aagggaagga aaataatgga aatccccata tttaatttgt
     5101 tgcgcggcat catagaaccg attgtaatgg aatggggcat cagcacccaa aattagttta
     5161 tgtgtgaata aatgaggcat gagaaatgcc aaagcaatga aaaaaattgc taaaaatggg
     5221 ccatattttt tcaattgtcg aactcctttg caaactttag ataaattcat catatatcct
     5281 atgcgagagg taggcaagaa attagaaaat tcatgaaaat atttatgaat tttacaagaa
     5341 aaacataagt gaaagttcta aggagagagg atgcgacata agttttgttg catctccttg
     5401 ttttgaaaca gaatttttct taatactcga aacaaagtag ctttttcatg acctctcggt
     5461 aaacggtgtt caatcagctg cttctcggta ataagctcac tagcctagaa aatatgagaa
     5521 gctattttct gactagcgct cttattactc aaagcataac gcagttttca caacctcttc
     5581 cttaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaacccaac aaaaaatatg
     5641 agtagctatt tttttgggct gtacttatta cttgatgcag gacagctttt tcacaacctc
     5701 atcggctttc attttctatt tttttcctga acccatataa taaaagaaag aacaaaaaaa
     5761 cttgcaaggg aggacacatg tgaagcagag taagcaaaaa cagtctcgtc gacttttgtt
     5821 tcgtttgttt ttattaatat gtatcgtcgt agggttactg atcgctttat atccatttta
     5881 tgtggattct ttaaatagtt ttattgatca aaaacgtatc gaacaagtac aaaaacggac
     5941 cgcagctgaa attgaagtgc agcgaaagaa aatggaggag aaaaaccaac gattaactga
     6001 ccaaggattc aatcctggta ctgatccttt caatgaaaag aacagaaatg aaagtacaac
     6061 atccagccaa ttagaggaat ggctgatcgg aagtatcaat atacctaaga tccaattaaa
     6121 tatttcactt tatgatcggc tcaatggtag gatccttgaa aatggagcag gcgtcttgca
     6181 aggaacctct ttcccgcttg gtggaaattc gacgcattca gtgatctctg ctcacagcgg
     6241 tttacctaat cgacggttat ttaccgaatt agatcgttta gaaaacggag acacgtttat
     6301 tttgactgtt ttgggggaga aattggctta tcaagttaaa aatatacaag tggtactgcc
     6361 agatgacacg tctgttctaa cgatttcaga aggagaagac ttagttacct tgcttacatg
     6421 tacgccgtat atgatcaaca cgcatcgttt gctagtgaca ggacatcgga ttccttataa
     6481 cgaatcggta aaaaaagaag aaggaaaagg aaatcaggaa agaaccatgc ggcaatggtt
     6541 tattcttgca ggaacgataa tagctgtagt catactgtta gtgtttatcg gacgactaat
     6601 ctatcagtat agattaagta aaaaagtgct tgatttttca tttattatta gtgattcgtc
     6661 agggaaaccg gtaaatggta gttcattcat tttgaaacat aagaaaaaaa cacttactag
     6721 aaatggtgtg ccgttttcag tacaaagcga ccatcatggg aaagtcaagc tggatcaatt
     6781 gcctggtgga acttatcgaa tcgtttcagc tgatgatcct aaagttgctg tttcttttgg
     6841 tattagaaaa gtaaaacaag agaagatata cttttttgag gggcgaaaac ttgtaaaaga
     6901 gcttcaaaaa aatggttttt attttaaaat caatgaataa gtaaggagag aaaaaatgat
     6961 ccgcacaaca acaaaattgt ctgccaaaga actggaatct gttctatcga tttggctgca
     7021 tgctaacttg gattctcatg aattcattcc atcaacttat tgggagaatc aagtagaatc
     7081 tgttaggaaa gctttaccag atgcagtgat ttacttagca gaaatagatc aagaaattat
     7141 tggttttgct ggggtgattg atcagtttat cgcaggaatc tttgtgaaaa gggaataccg
     7201 gcaacaaaga atcggtacgc aactaatgaa aaaaataaaa gaaaccaata aaacactgac
     7261 tttgacagtc tatcaaaaaa atcaatcagc tattcatttc tatctttcac aaggttttac
     7321 aattcaaaca cagcaaaaag atgaagcagt aaatgaggag gaacttgtga tgaaatggaa
     7381 tcaaaacgct gatttttgat gaacagaata acgaaataat gctcccagcc taattttttg
     7441 ctgggagcat tatttcattt tgaagtcact aatttaactg tgtgaatatg cagttgttac
     7501 aatctttgat aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa
     7561 atatgaggaa ctatttttcc atttgtttct ctaatactca ggtcaaaacg ctgatttcac
     7621 aacctcttga taaacggtgt tcaaacggct gcttctcggt aataagccca ctagcctaga
     7681 aaatatgaaa agctattttc taactagcgt tcttattact caaagcataa cgctgatttc
     7741 acaacctctt gataaacggt gttcaatcag ctgaccctcg gtattagctc aaaaaacggc
     7801 aaaacatgag gaactatttt tccgtttctt tctctaatac tcaggtcaga acgctgattt
     7861 cacaacctct ctttcacaac cttattttac tcgtttataa ttttcatcga agtattttcc
     7921 gctacggata tcatctggca ttccatcgta tggttcttcg ctaataacat cttcattgtc
     7981 tatacctgca tcacccatat aagtgatcgt agcaaattca ggaacaagta attttggata
     8041 agtttcatat ttttcacgag ctgcttccat ttctgcgata tgttcgtttt ggaaagtaac
     8101 agtgatttct ggatgttctt caacccattt agggaaaaag atcattccgt gaagtttctt
     8161 ttcatttggc ataaagtcca atgccacgtc tgtccctgtt ggctctgtcc aagtgatttt
     8221 gaatacgccg tccgtcaatt taacgatatc agcttcttgg tcttttaccc aacggccttt
     8281 gaccattccg ccatggatac ggtaatcaac agtatggtca tttttggcat accattcata
     8341 ttcccaacca ttgtcatacg tatagataaa gtgagttcca ataaaatctt ccaattcttt
     8401 aaattctttc atgtttaagc atctccttat aaattaaaac atgtaataaa ctacatgttt
     8461 tcgattactc atttatgata gaatggattt aaagatttgt caaggaggga aaactgatgg
     8521 caagaaaaaa tacacttcga tatattttgt tagggttatt aagtaagaag aagatgacag
     8581 gatatgaatt gaatcaatcg ttcaaaaatg aaataggaga attttggcaa gcaaaacata
     8641 gtcagattta tcctgaactt gcaaaaatgg aagagcaagg aatcattcag catcaagtag
     8701 agatcacagg tgaaaaattg gaaaagaaag tttatgaatt aacagaagaa gggaaggaac
     8761 aattggcaga gtggattcat acacccacca atgaactacc ggttaatcga gacgaatttg
     8821 ttctgaaatt gtatttcgtc aaagacgtga atgatccggc attagtagaa attattcagc
     8881 agcagcgtga ccttcatgaa gaaaaacgac ttcatctatt agagcgaaaa aaaacgatct
     8941 ttcctactga aaaagaaaaa catgaaaatt acggacacta cctgatttta gcgcatgcga
     9001 tcaatcggga aacagaatat gcaaattggc taacagatgt attagaagaa agtaaccgaa
     9061 aaaaataaaa aaatatccga acagtgatgg agaattcctt tctatatgaa agaaacgcag
     9121 atcaccgttc ggatattctt atgttcaaac aggccttttt tcaagtattt atgtagcatc
     9181 tacttattaa aaagcttggt acttaggaga ttagtggcgt ttagagttct tttaacaact
     9241 gttcgtagtc agctttttca tcgacaggaa ggagcttgta agttaattcg tctgtctcgt
     9301 tgtcatgaat gatgtacatc ttagcattct cttctttgaa gttaccgatt tgtaaaatcg
     9361 tatagcgttc gtcgatcaat tcgatatctt tgattgcttt tgaaaaaact ttacggacat
     9421 ggtgcatttt cttttggcgg atttctgcct tttctgcagc ttttgacaaa gtaaatccgt
     9481 catctaaata ttttttgatc agttctactt ttatgactgt aggtaaacga tattttctag
     9541 gacaattcgc atcagcttcg atcgaacgaa tgaatccctt ttgttcccaa taccttaatt
     9601 gacgtggaga cacacctacg atatcactta attcactgat gccaacaacc aattgatcat
     9661 tttccaacaa acgtttaagg gaagccccaa ccatttttgt cactcctttt gtcctcttgc
     9721 ttcccttaat tatatccgta ttaacttatg tgtcaagtag gttgacaaat agcgaattta
     9781 ggactaaagt attctagtag tttcagaaaa aattcagaaa gaaggagaaa tatgagtaac
     9841 aaccaacccg tagatattta cggaaaacca tataatcgga cacttttagt tgttgtctta
     9901 ttaattggta cattttgtac cgttttgaat cagaccttat tgacgacagc ttttcctaca
     9961 ttgatgaaag ctttcgatat ttcagcgtct gatgtgcaat ggttaacgac tggtttcttg
    10021 ctagtcaacg ggatcatgat cccaataaca gcatggctga tcaataaatt cagttcaaga
    10081 aatttatatc tttctgccat gacgatcttt ttgatcggaa cgattacttg ctttacagca
    10141 cctaatttta gtacattact aatcggtcgt ttgatccaag cttgcggtgt gggagtatcg
    10201 atgccattat tgcaaaatat catgctatct attttcccgc cagaaaaaag aggatcagca
    10261 atggggatgt cagggatcgt tattggactt gctcctgctt taggccctac attatctggt
    10321 tatatcattg ataactacca ttggcgtgac ttgtttggta tggtactgcc aatcgttgtt
    10381 cttgtcttgg ttttagcatt cttcctaatg aaaagtgtta ttccattgtc aaatcctagc
    10441 attgatattt tatccgctat tttatcaacg attggattcg gtagtttact ttacggattt
    10501 tctagtgtag gagatgacgg ctggggaagc ttgaaagtaa ttggtttctt agttgttggt
    10561 gtgattttca tcgccttgtt ctcatggcgt caattacatt tggaacatcc attcttggaa
    10621 cttcgtgtat tcaaatcatc tacattcacg attgcagctg ttttagctgg tgtaacaaat
    10681 atggcgatgg taggggcaga aatggtcgta ccattgtata ttcaaaatat tcgtggagaa
    10741 tcagcattcc attctggctt gatgttgttg ccgggtgcat tgatcatggg ggctatgatg
    10801 cctgtgacgg gtgcgatttt cgataaatac ggcgcgaaac gtttagccat cacagggatg
    10861 ttcattttga cagcggcaac tgcaccattt gctttcttga caaaaacgac tccagtttta
    10921 tatatcgtga tcctttatgc gatccgtatg tttgggatct ccatggtcat gatgccggta
    10981 acgacttctg gtatgaatgc attgccaaat aacttgatca gtcatgggac agcggtaaat
    11041 aatacattcc gtcaaatcgc aagctcgatt ggtacagcta ttttgatcag tgtgttgacg
    11101 aatgtgacaa aagcaaatat cccaagcaaa tctgtattgg ataatacgcc attagcttat
    11161 aaagatcagg caatcaatgc cacactgtca ggttaccatg ccgcgtttat cgtagcgatc
    11221 atctttggga ttcttggctt tattattgcc tttttattga agaaaaaaga acctgttcta
    11281 atgaaagcgg gggataatgc atgattattg gagttttgat tgtcagcttg cttgcttttg
    11341 ctttaacgaa cgtgttcgct aaaaaaacat ggcaaacctt tttatctttg attttcggac
    11401 taatcttcgt cgcttcacta agtttgatcg tcgctaattt gtccaatcac tttggaatgg
    11461 aaaaagtaac ggaaacaaaa acagaaaaaa ttgtttccag tgctgattcc caaggagcag
    11521 atatgctttt gtataaagct ttagggaatg gaaaagaaaa agtgtatctt taccgaacaa
    11581 atgaaaaaca agaaaaacca aaagcaactg gtacggataa cgaaacaaat aaagtagaaa
    11641 agacagacgg cgatgctgaa aaagtgacga aaactactta ttgggaatac aaaaacgaca
    11701 tgtataaatt ctggttcaat attgctgata acaatcacga atatgacagc cgagtgaata
    11761 cattcaaaat cccagagaca tgggtagaat taagtacgga ccaagcagcc aagttagctg
    11821 aattagtgaa gaaacagcaa agtacgatgg aaagtgaagc aaaagcttat gttcaagacg
    11881 gtatggtaaa agcaatgact gaaaatccaa aaatgagcaa agcagagcaa gaacaacgta
    11941 cgaaagcctt agctgctgaa ttccaacaac aagcattcgc aaaattagtg aaagaagcaa
    12001 aaggcgaata atattttgaa caccatgtag gaggttgtga aaaagctgct ctgcatcaag
    12061 cagtaagaac aatcagataa aaatagcact ccatattttt catctgattg ggcttagtgc
    12121 cgtagatgca agcttttgaa caccgtttaa ggaagaggtt gtgagcctgc cgtttaactc
    12181 tgagtcacaa ggagcaattt caaaaaacag cttctcatgt ttgttgacta ttgtgacttg
    12241 tgaccgagga gttggcagga gaacaccgtt tattcgaaaa aaggaactgc gacaagactt
    12301 ttgtcacagt tccttctttt ttttgctatg ctatactaga tgtaaaaaat aggagaaata
    12361 aaatgaggga aatttattct tttgaatcaa aaatctatgc ttccgaagtc ggcaagggcg
    12421 gggcatatat tatttttccg tatgatataa gaaaagaatt cggtgctgga agagttaaag
    12481 tccaagcaac ttttgacaat gtgccgtatg aaggcagtat cgtgaatatg ggcgtgaaaa
    12541 atccagatgg ttcagtctgc tacatccttg gactacgaaa agatattcgt aaaaacatcg
    12601 gaaaagaaat cggtgatatt gtagcagtta cagtgaagca aaaataaaag aggttgtgac
    12661 aaaagacgtg tcacaacctc ttgataaacg gtgttcaaaa gccagtttct cggtagtaag
    12721 tcaaatttat gcaaaaatgt ggaaacccat gtttacaaaa tttttcttac tactcgaaac
    12781 gaagcggctt tatcacaacc tcttgataaa cggcgttcaa tcagctgtcc ctcggtatta
    12841 gctcaaaaac ggcaaaatat gagaagctat ttttccattt ttttctctca tactcaggtc
    12901 ataacgctga tttcacaacc tcttctttca taatggtttt tcaccatctt tataataaac
    12961 tggttctttg atatggaaat ctattttttc caaccaatct aaaatcttgt aacctaactc
    13021 ttctgacatc ggaaaagagg cgttgatttg tgcattggag atatggtcaa catggatacc
    13081 ggaagtgatc gtacaacttc ccggaacggc agttttgatg atcacagcta ttttttcggc
    13141 taagacatcg tctttatgga accgaccatc gtggctaggg aaacgtacag tttgcatctc
    13201 tgtatcacga gaaaatgtag tgactgttcc gatatgtggt gtatctccgc ctgttaaagt
    13261 gatcaataag tctttaccga ttactttaac agcagcttgg atcgtgaaac ctagtttggt
    13321 tacttcaact atcgtttctt tttccatatt aatcaccctg ccaacgtttt gaatgctcat
    13381 ttacaatttc aaattgatcc aaaagtttca ttgtatggtg attaacaaga tcttggatcg
    13441 tttgaggctg attataaaaa gcaggaattg gtggtatgat ttgcacacct agtcgtgcta
    13501 atttagtcaa gttttccaag tgaatggcat ttaatggcgt ttcacgagga acaatgacta
    13561 actttcgttg ctccttcatt gttacatcag cagcacgtgc aatcaggttg tcgcctaaac
    13621 caacagagat acctgcgact gttttcatac ttgccggtac gatgatcatt ccatcggtca
    13681 aaaatgatcc gctggcgaca gcagcaccta aatctttgtt tgaataatgg taatcaaaca
    13741 ggctttcgaa ttcagttagt gataagtcgg attccagctt taagttctgt tttgcccatg
    13801 gactgatgat tccgtgtgtt tctatcatcg gatattcttt tagtttcttc actaggttga
    13861 tggcataaat ggtgcctgaa gcgccactta caccaatcac gatttttttc ttccgttgtg
    13921 ccaattgttc cattttccgc actccttttt atgatgtcaa aacagttgca gatcattcat
    13981 tatttcaaat atttttccca atcttctact tcttggaatt ttgcacgtac aaaatgttct
    14041 ttcatatcga aaggaactgt accgtcaaag atagtttttg cacttatccc acggaaacga
    14101 atagagccag gattatattc tggtaattct gatgggtcca atggatggtt cctcattcct
    14161 gggatcacga tgatatcttt atctccttgg aaacgagtgt tgaaagtcca aactacatcg
    14221 ttcatgtcga aaatatcaac atcttcatcg actaaaatga ctgtcttcaa ttctttgaag
    14281 gctgataaag caagaatagc tgcttgacgc tgaatacctt catccgcttc gttttcttta
    14341 cggatttgca tgatggtcat caatttacca ccacctgcag gaggattgta gacatttact
    14401 acttttcctg gaatagcttt atcgaccaag gcaagaatac ttgcttctgt aggaataccg
    14461 gccatagata cgtgttcttc actgggacca atcgttgttt gcatgattgg attgtctttt
    14521 cggtgagtaa ctgctttgac tttcactact tgtacagctg gattagcatc cccagtatag
    14581 ccaggaaatt ctggcattgc ttttcctgag ttcgtgttga tatcttcttg cattgtttca
    14641 tttggcatga tttctgcttc aatgatgaat tcagcacgag caatacccac ttcatcgact
    14701 gctataccat caacgatttg aataggttcg ttacgcaaag ctcctgcaca ccatagttca
    14761 ttgtagccaa gtggagtggt aggtggttcg aaagtcgtgc caatggcaat tgctggatct
    14821 aaaccaatat tgatagtgat aggcattggt ttattcaagg cttcgaattg tttttggaag
    14881 tgaccaatgt ggcgtccgcc aggcatgatg tacataccga tcgtatcttt atcttctaga
    14941 accatacgat ggatcgttac atcgctcatt gttttttctg gatctgatcc taagactaca
    15001 cccattgtga tgtatggacc agcatcttca ggagtatttg taggtgcagg caaaatgttg
    15061 cgaatatcga agtctgcatc ggtagctaga tgaacgactt cttgtgctgg agcgtctgct
    15121 ttgtctactt tcacaggttt gattggattt tctacagcgt ctttcaagaa acgaccaagt
    15181 gttcgataat catggtgcaa aatttcacct acacgtttac gactagccat tgttccgatg
    15241 ttcacacgtg tgccggggaa acctttgata ttgttgaaag tcatagctgg tccttcttga
    15301 gtcggacgtt ttactgtacc gccagcaccg atataacggt aaacaccaga taattctgct
    15361 tcaggatcga tttcaatatc tgtttcgtga tattgacctg gaagtgtttt tagttcttcg
    15421 atgacttttc ttaaatcata cggttgatct gtcatgagag attcctcctt agttgtcttt
    15481 acctatctat agtaaaaggt gaaaaagagg aaaacaattc aacttgttcg gtaaagtatt
    15541 ctacaatgga atggatgcgt ttttttgata atagaataaa ctatgatata cttatcacta
    15601 aaagaaagcg catacttagt gttggaggaa ggaaagtgaa aaaaatggag gagtcatttc
    15661 ctaaagcagt aaaagtggaa aatatagcga atattttgaa agttacattt gaaaacggag
    15721 aagtaaaata cgtgaaatct cattggacag aagagataac cgatgccttg caatttggaa
    15781 aaaaaggaag aggaaaaaga aaaaatcttc tcgcactttc tcgtaatatg tggattggga
    15841 ctgaagtaac gattgaagca gatggaacag tattcataaa tggaaaagac aggtatacgc
    15901 cagaagagtt gtggtacaaa ggaaagaaaa gtataccaga attataaaaa ataattaaaa
    15961 aaaacagcgc gaaacaagga acatattact tcctcgtttc ttgctgtttt ctactatttc
    16021 agcgctctta tgactcaaat ctaaatgtgg ttgtcacaac cttattctgc acgcaaagcg
    16081 atagcggcgt cttttttcgc tgccattcgt gcaggaagat gaccgccaag catcgtcaat
    16141 atagtagaga cgatcactag aataatcgca tggacaggat ttagttgagc aacatttttt
    16201 agatccgtca tgttgtataa gatgctattg atagggaacg tagctagcca tgcaataaac
    16261 acacctaata tcccagaaga aatacctaaa atacacgttt ctgcgtcaaa tacgcgagtg
    16321 atgtcttttt tacgtgcacc taaagctttt agtacaccga tttcttttgt ccgttcaatc
    16381 acagatgtat aagtaatgat actgatcatg atcatacttg ttactaaaga gatccctgca
    16441 aaagcaataa ggacataagt gatagcatcc atcaatccgc cagtcagttc agtcatcgtt
    16501 ccagctaaat cagtataaat gattttatct tcatctgatt tgcctttatt gtaatcatct
    16561 agataatcca agatcttttc tttgtcttca aaattgttcg gataaatcat gatacttgaa
    16621 ggcaagctgt ctccacctaa ataactaagc aatgtttgtt tggcattttc gtcaactttt
    16681 tcagtagtta agacatttac accactgtct ctttgagctt tgacgatttc tgattctttg
    16741 ttttcgttga cgatttgagt cgttaattga tcgctataag cgattcctgg agataagaga
    16801 ttcattgtag aagaagattt cacgcgtagg atccccgaga tcttcaattc atcagaagca
    16861 ttctgataca tcgcatcata atctgtatta gggataaaat tcccagttgg aagttttgta
    16921 tagtaagtat tgttgtttac taatttgaat gttgtaccaa cgatatcatc aaaatcaagt
    16981 ttttcgtctt ctttcacatc aaatccaaga ttcttcaatg cgttgatatt agtattatta
    17041 ttgccgtcta cgatcagcac gacatctgtt gctgaggctg gataacttcc ggcaagtaaa
    17101 gaataattgt cttttaaaaa gttttctttg ctagtgtcaa gctgtgtagg gaaacttgac
    17161 actcccacac ctgtcattgc tgacatcgta ctagataaag aaagagattc cgtatcagga
    17221 ttttgattcg agaagctgac aggctgtact ttgccattta catcacgtaa aagattgatt
    17281 cctgtagtac gggtaaatcc gatattatta cttaagttag gatcgatgtc tgttacgtaa
    17341 tcgacgtatg tttgatcaat cttattcgtg tgctgtgctt tatcttcttc gctgactttt
    17401 gcagtaatcg ttttgctgtc tggataatcc gctttgcttg cacccaatcc ggcatcgtca
    17461 cgagtctggt tagtcgttac cttagaaata gtaatcggaa atttcgccat cgtttcagct
    17521 tgcgtgttat cgatttgttt ttggaatcca gaagaaaggg acagaacgat cgcgattccg
    17581 atgatcccaa tactggaagc aaaggaggtc aagaaagtac gtcctttttt tgttcggata
    17641 ttgttgaagg aaagctttaa cgctgtccaa aaactcatct ttgttctgcg taagttaaat
    17701 tgatcatctt ttggtcgttc gatgtgagga tgggaatctg ttaagatctt accatcagaa
    17761 aattcaatga ttcgatctgc gtattgatgg gccagttcag gattatgtgt gaccatgatc
    17821 actaattttt ctttcgataa ctcttggatc aattccatga tctggatact tgtttctgta
    17881 tctaacgctc cggttggttc atcgcacaaa aggatatctg ggtcattggc tagtgctcga
    17941 gcgatggcca ctcgctgcat ttgtccacca gataactgat ttggcttttt atgcatatga
    18001 tttgtcaagc cgactcggtg caatgcatct tctgcccgtt ttcgtttttc gtctttagaa
    18061 acaccactta atgtcatccc aagttcaacg ttttcaataa tgcctagatg accaatcaag
    18121 ttgtaacttt gaaaaatgaa accgatagag ttgtttcggt aggcatccca gtcactgtcc
    18181 ttaaagtctt tagtagactt tccgttgatt accatatcac cggaatcata attatctagt
    18241 ccaccaatca cattcagcat agtcgtttta ccagaaccac taggacctaa aatcgctacg
    18301 aattcttttt gtctgaaagc gacagacaca ccatccaatg ctttggtggt ggtttcgccg
    18361 actttataat atttttttat atcttttaat tgaagcataa tcacccttct cccgtattga
    18421 aatattcggt atactaataa gtatatcaga acaatgatcg tagtatacga aataaatgtt
    18481 aactgaagat gaactacaag aaaaaatcta gaaatttttc aaaaaacaga gggagatcaa
    18541 tgaatcgtat tttagtagta gaagatgatc cgcaattaca gttgctttac cgttctgtgt
    18601 tggaacgagt gggcttttca gtcatcaccg cattgaatgg agaggatgcg ctgaaaaaac
    18661 tagaagcgac acgtatagaa ttaatcatta cagatatcat gatgccgaaa atggatggct
    18721 atgacttgct agactcatta cgtagcacca gatttgacac acctgttcta atgattacgg
    18781 caaaggccga ttttgaggat aagaaacgcg ggttcaaatt aggagcagat gattatatga
    18841 caaaacccat cgatgtcaat gagatgatcc tacgtgtgga agcattgctt cgacgttcaa
    18901 agatcaatta cagcagtagc ttgataatcg gacaaacaaa attagagcta gatgcttata
    18961 cagtgttaca agatcagcat tcgttgattc tgcctcaaaa agaatttctg cttctgtata
    19021 aattactgtc ttatcccaac aagatcttca ctcgccaaca gttgatggat gatatttggg
    19081 gattagatac gaatacagaa gaacgaacga tcgatgtaca tatcaaacgt ttaaggaccc
    19141 gttttgaaaa gacggaagat tttcaaatca tcacagtacg gggactaggc tataaggcgg
    19201 ttatttaaag atgaaaaaaa agctattttc acaattatgg atctattttg ctagtattgt
    19261 cttcatttcg atcttagtga cactgctgtg ttttctcggt tttattttct tattgtctcg
    19321 acattctatc tcgcctgctg aatcggctgg aaaaccaacg ttatacccgt tattcgtctt
    19381 tgcggggttc agtatggtcg tgggtacagg aatatcgatt tttgttgggc ggcgtatcct
    19441 gcatccaatc agtgcgctag ggacaaatat gagtctggta gcaacgggag atttttcgat
    19501 tcgaatggat gaacagcaga aagtagcaga agtacagcaa ctgtataaag atttcaacgt
    19561 tatggtccaa gaactcaata gtatcgagac actgaggaat gactttgtgt ccagtgtatc
    19621 ccatgaattc aaaacaccac tagcaacaat ccaaggatat gttcaattgt tacaagcccc
    19681 aaatctctct gacgaagaac gccaaatatt tttatatcgg attatcgaaa gtatcacgca
    19741 gctttcacaa ttaacagaga acatcttaaa attgaacaaa ctggaaaacc agcgtatcca
    19801 actggaaaag aaagagtatc gattagatga acaaatcaga gaagtaatcg ttttttttca
    19861 gcctaaatgg gagaaagaac agctagaatt ggatatcgaa cttgctgcag taaattacac
    19921 aggcaatgaa gagtttttgt accaagtgtg gctgaatatc atggataacg ccataaagta
    19981 caatcaaatc aatggacaga ttcatatcaa actatttgag acagccacag agattgtact
    20041 ggaagtgacc gattcaggtg taggaatgaa tgaagagacc agagatcgaa tgtttgaaaa
    20101 attttatcaa ggagatacaa gtcgtcaaat ttctgggaat gggctaggac tttcactggt
    20161 taaaaaaatt ctagaactgc atgatggacg tattgattat agcagtatcg aaggagtagg
    20221 aacgacagca atgatacggt taagtaaaca ataggaaaaa gaataaaacc cctaaaaatt
    20281 ctattgaagg tttttagggg tttttagtcg tttttttgtg attagggaaa tgttcttacg
    20341 gttcttttgg cataaaaatc gctgctttct tattgaacta cgagaaatgt tagaggtgtc
    20401 attgaaacaa ggacatgatg caagaaagag agcgaaagtc atgcgtcgct ctcttttttt
    20461 gggtggatag atggacagaa aatgtagggc ttagttttct tcttgattta aatactccca
    20521 aaatgtatct gaagccaaag aatgctcacc gactttacga gtaaatgcaa gataactctc
    20581 tgttgttgga gaaataggga gaatcacagt attttctttt aatagattct tgactgtttt
    20641 ttccataaca atagcaatcc ccatgtcatt tgcaatcata ttgatgatca agtcgattct
    20701 tgctccttta taagagattt ttggttcaaa tccagcttcg cgacacagat ctagtacagg
    20761 ttgatacaag ttcgtagtgg ggccaaggag caaaaagttc tcctttttca gttcgcttag
    20821 atccaatttc tttttactag ctaaaggatg atttttaggt agaactgcta caaaatcgtc
    20881 tgtttcagta ggcaaccact caatcgaact tggtggggcc tggaaacttc tagcaaagtg
    20941 gatcgtgtca ctatgttcat tggcgtcatt gtaagttaat tcgacacttt ccacttcttt
    21001 caactggact gtaaattcag gatggcgttt caaaaactcg gtgattttgg aaaaaccttt
    21061 ataattgatc attgtcggta tcgttaagat atttaatgaa agattgttgt ctttcgtata
    21121 ggtttcaagc acttcttgca tagcatggta ttgttctacg acatttttta catatgggag
    21181 caataagctt ccagcttcgg tcaattcgat ttttctatgg gcgcgttcga ataatttgac
    21241 gtctaattct gtttctaacg aaagaatctg tttagaaata tttccttggg tggtgaattg
    21301 tcgatcagca gttttggtga aattcattgt ttcggctaaa tcaagaaaaa cttcgaattt
    21361 atggatatcc atcatattcc tcctacttat tccaataaag aatactttaa cacaaaaaat
    21421 gaattggtgt aatcttctct gtctgttaca atctttcttg cagcgaaagc aataattaca
    21481 gaaatttttc tgattttttt cgcttcgtag aaaaagagag gagttttttg atgccaactg
    21541 gagttttgat caatgtaggt tccgttttgt ttggcggact aattggcgga ttagtaggaa
    21601 ataaattaag tgaacatttt aaagcacagc tgacgatggt ttttggtgtg tgttcgatgg
    21661 ggatgggaat ctattccatt gcgccgatga aaaatatgcc ggctgttatt tttgctttag
    21721 tgataggaac agcgattggt ttgattgttc atttaggtaa tgggataaat aagggggctg
    21781 cgctgatgca agtacctatt tctaaaatct tccctagtga aaagcttggt atgacccacg
    21841 atgaatttat cagcacatta gtgacggtca ttgttctatt ttgtgcaagt ggtacgggga
    21901 tctatggaag tttagattct gggatgacag gagacagtac gattttgatt tctaaatctg
    21961 tccttgattt tttcacagca gctatttttg cgtgtaattt agggtatgtc gtttctgtga
    22021 tagcaattcc ccaattcatt attttttata ttttgttctt attggcaaaa tttatttatc
    22081 cattgactac accagacatg atcttagatt ttaaagcttg cggcggattc ttgatggtcg
    22141 ctaccggttt tagaatgatc aacgtgaaaa tgttcccagt agctgacatg atacctgcaa
    22201 tgatcgttat catgccactg agctggatgt ggacaaattg gattatgcca ctgttataat
    22261 aaaaatcaaa gagaagccat tttttgctga ctaaaaagga tcaaatcttt tagtcagttt
    22321 ttttgtattt gacaaactga ttttgaaact atattatatg ttgtataata tagattgaaa
    22381 taaaatatta cacttgagaa tagaaggtga cgtaatgcac atccagattc caactgttct
    22441 tcttgatggt actgtgctag cagcattaag caaggaagat atgtatgggt atgccttaac
    22501 aaaaatcgta caagaaagtc tctctgtcag tgaatctaca ctatatccgg ttttgagaag
    22561 attgaaaaaa gaaggatatc ttgagaccta tgatgaacct tttgaaggca gaaaccgtcg
    22621 atattactcg ttgacaccaa tgggaagtcg tcatttagaa gaaattattg atgaatggat
    22681 tgtttttcga gattctttag accggttatt ggaggagagc acaaatgaat gaatatttga
    22741 atgaagttgt ggatcatctt caagaaattc ctgaagacga tcgttttgat ttgatccagt
    22801 attatgagga atatttttta gattctggaa aaacattaga tcagatcatt gatgaatatg
    22861 gtacaccaaa gcagtttgcc ttaaaattaa aaatcagtta cttttcagaa gaagaacaag
    22921 cagaaaacga gttagtgaaa aatccaaaaa gacaaattcg cctgatttgg ttaatcatta
    22981 tcggtttatt tgcttctcct gtcctcattc cactagcact ggggtttata ttggttattg
    23041 ggggactatt ggttgcttta ctagcgctca tttttagtat ctatgtttta tgtatcagta
    23101 tcttaggtgt tggcctattc atgatcatct caggaatcag tgtgtttgga caatcgattc
    23161 tgagtggatt attctttatc ggtgtcggtc tgttcgccac aggaagcgca gtatttattt
    23221 cgccactgtt gctgaaagga acaaaagggc ttttttatgt catgatgaac tttgttaaat
    23281 ggatgggacg acgttttatt acaaaaagag atctgcattc agcgggcgtt tgaaggagga
    23341 cacataaatt gagattgaaa cattttttga ttaccggtat tggtctgatg cttactggtg
    23401 gcctcctagc aggtgcaact tatacattag gcgctcaaaa gagtctgact tgggataatg
    23461 gtcctcggtt gatcgatatg gttactgaga caaaaaaaat caaggatgca aacaaaatcg
    23521 tcatccatgc agaaaatcaa acagtttcgg tcatacgcgg attggatttt gagataaaaa
    23581 cgacttatga taagacagaa aagccagaaa taaaggagaa aaatggcgtg ttgactgtga
    23641 atgcagtcaa aaaatcaaca ggtgcaatca ttagtttaga agacacaagt gcacaaatca
    23701 ctattgttgt ccctgcagat catgaattat ctgaattgac aatcgaaggg aataatagtg
    23761 caattgatat caacgaagta tcaatcaaaa aagtgaatac taagacacat gatggctggg
    23821 tatcgttcaa tgaatttaca ggaggaacaa taactgcgaa agatgaatta ggctcacttc
    23881 gattagatga ggccaatgtg gataacttga tcgtcacagg tgaaaacagc aacttccatc
    23941 tagaagacag tttgatcagc aaagaaggaa aagtcatcct aaacggtggc aatttatctt
    24001 tgcatgagat ggaaaacagt ggctatacta tccaaacgtc tggtgaatct tatattgaaa
    24061 aagactacca gccaataaca aatactttgt tagaacaagg acagaaaaag ctaaagatca
    24121 ctgctaaaga cacatcgatc aatattacga cggatcaaga ggacaatgaa acagattgat
    24181 cctgtactca actggatatt ttaagaaaaa acagcaaaga tccttccaat ctttgctgtt
    24241 tttcatcgct taacgcacat aactatagta aaataagaaa gaaaatatct aaaaagaaaa
    24301 cagggtgaaa gagtatatga aggtagaaga aggtcaaaga gaaaagttaa aaacagaaat
    24361 ggaaagatta catacttata tcactcaact aagccaaacc ttctatgatc cagacaaaga
    24421 aaaagtgatg gttaattacc cgaacaattc agaaggtcgc caattagagc aagtgtacca
    24481 tgaagttttc aaacatttat tgacggtgaa gaaagagctg gattattatt ctttaccgat
    24541 tatcgataca ggaatattga agtatgatgg gaagaaggaa cgatttatct ttaaatctgt
    24601 tcgggaagat ttgcctcttt cagcagggat ggatctagag gtcttggtag aagactattt
    24661 tacagaagaa aagcactggg ttcgaacaaa attggattat ttaccgcagg cagccggagg
    24721 gacacaggca agcggttggt acatcactga agacaaagag ttagaactag aaggtgtaat
    24781 ggcgcgtatt cgaaaaaaaa attaaaaaag taacattaga tatttattga aggataaagg
    24841 agcgcaggaa gtagcatgag agaaaaaaga gcattttata tcaatggtta tgcagggatt
    24901 gtcggattgg tcgtattagc tttagttgga ttgtttttct tctatttagg tatgtggcaa
    24961 gcgaaagttt gggcactttt tttaagtatt tttctttggt tgatcacgtt attgttgtta
    25021 agttcggcaa ctgtagtcag tcctaatcaa gcaaaagtaa ttttgttttt tggacaatat
    25081 ttaggtacga ttcgagaaaa tggctttttc cttacgatac ctttggcaca aaaaatgact
    25141 gtttcgttga aagttcggaa tttcaatagt tctgttttaa aagtcaatga tttggatgga
    25201 aaccccatcg aaatctcagc agtagtagtc tttaaagtcg ttgatacagc aaaggcatta
    25261 ttcgatgtag cgtattatca agattttgta gaaatccaaa gtgagacagc cattcggcat
    25321 atcgctagcc aatacccgta tgatacattc aatgagaatg atttgacgct tcgaggaaac
    25381 acgacggcag tgtctgacga gttgcaaaaa gagctgcaag agcgtttagc agtagcaggt
    25441 gtcgaagtaa tcgaaacacg cttgaaccat ttagcctatg caacagaaat cgctagtgcc
    25501 atgctccaac gtcaacaagc aaaagccatt ttgtctgctc gacaaaccat tgttgaagga
    25561 gctgtcacaa tcactcaaat ggccttagag caaatcgaag atggacaaga aatcaatttc
    25621 accgatgatc ggaaagtcca attgatcaat aatttacttg tctcaatcat tacagacaaa
    25681 ggaacgcaac ctgttatcaa tacaggcgac atgaaagacc gtaagtgaca tactttttgt
    25741 aagataggaa gaaggagttt aggacggatt attgtcttga actctttttt tagttgtttt
    25801 aaagaaaatg acaaaattga aagaagaatg atagttgatt cgaaggcaga attgttcgct
    25861 tttttctata atcagtatat aaagtaagag agaaggggaa aaagtgaaaa aacttctgat
    25921 cggtctactt gttttcatcg ttgtggtact tgcaggggtg aaaatcgcag attacgtagt
    25981 tatgggtgga gaagaatact atgtacaaat tacgacagat ggtacaaaaa cagtttcaaa
    26041 atcagactct ggacaagaaa cagtcaacta tagatatatc ctgcctggct atacgaaaga
    26101 gggtaaggaa aaagaactgg acttcaaggg acaattgact cgaccgcttc gtaaaggagc
    26161 atatttaaaa atcacctgga acaaaaataa aggtgtcact tcgtatgaag aagtcgaaaa
    26221 aaaagacgtg ccgaaaacag cactggaaaa actaattaaa gggtagggaa agaaatgaac
    26281 aaaatagtcg aagtacagaa tgtaacaaaa gtatacggaa aaaacaatga gaaaaagacg
    26341 caagcattaa gcggcatcag cttcgatgta gaaaaaggcg aatttattgg gatcatgggc
    26401 gcttctggtt ctggtaagtc gacacttttg aatattttat ccacattaga taaaccaaca
    26461 gatgggcata tccgaatcaa tcaaaaagat gtgaccacat taaaaggaaa tcaactagca
    26521 gattttcgtg cgaatgagat tggctttatt ttccaagatt tcaatttatt agaaagcttg
    26581 acagcacaag aaaatatcgc tgttcctctt tctttacaag gcgtcagacc aaaagaaatc
    26641 aagcaacgtg tccaaaaaat tgcagaacgt ttatccatct ctcatatttt ggaaagctat
    26701 ccttctgaga tttccggtgg acaaaaacaa cgggtcgccg ccgcacgtgc attgatcaca
    26761 caaccaacga ttctattagg ggatgagcct acaggtgcgc tggattccaa aagtgcccgg
    26821 gatcttttag atacgatgga cgaattgaat acaaaagatc atgtatcgat tcttcttgtg
    26881 acgcatgatc cgttttctgc aagttattgc caacgtattt tattcatcaa ggatggcggt
    26941 atccatcaag aagtgaaacg tggagatcaa agccgtgatt ccttctatcg agaaatacta
    27001 tctattcttg gaaacttgga acagtagggg gagattgact atgctttgga aattatcact
    27061 tactgggatc aagagccgtt taagagacta cttggtgtta ttctcaggat tagttatggc
    27121 atctgctatt ttttacatgt ttgaatcaat ggcaagcaac gaagcttttt tgaaaagtaa
    27181 tacgatcgtt gcggctacag ttatcatttt ccgattcggt tctgttttac tagggatcat
    27241 cacattcgtt tatattttat atgcaaattc ctttttgatg acgatgcggc aaaaagacta
    27301 tgcaatgttt atgatgctgg gagcaaaagg cagaaaaatc gcacagatga ttttttctga
    27361 aacattctta gtagggatcg cagctacttt agtgggttca gctgtcggtg taggattgac
    27421 aagtatcgtc caccgattat tagtggatca gttagatctc aatatttcgc attttacacc
    27481 ttttagtatt cacgggttgt tgatcactgt tttgtttttt gctgttttgt ttttattagc
    27541 tgctgttgtc aatgcttttt cgattgtcaa aaaaccgatt ttagcattga ttcgtgcgga
    27601 tcagacacca acaagaatca agcagcataa attcttgttt ttccttgaag tagtactggg
    27661 aattgctttt ctaggctgcg gttactatat gatgaaacac gtggggacct atcaagtctt
    27721 tggtataggg gtagccttag ttacgatcgt attaggaact tatttcattt tccattctgt
    27781 gatcattttc tttttatcat tactaaagaa atcagaacag atttcattga aaaaattgaa
    27841 caatttcaca ttgtcacagt taaactttcg tattcgtgaa tatacacaaa tgctctcaat
    27901 ggtcgctatg ctgtttgcct tggcactggg cgctttgacg gtaggattag gttttaaaaa
    27961 tgaaattgca gacttgacag aaaacaattc tggatatgat ctagtgatga ataatcctcg
    28021 ggcagaagat cagcaaaaaa tcaaagaact ttcaccaaca ctttctgctt cttatactca
    28081 aaaagaagat gcgggaacga tttactataa tgcaagtgaa tttgcggctc aaccattgat
    28141 catgataaaa catgaagatg gaacaccgcc gaagatcacg aaagaaaaag ttcctgctaa
    28201 aaaaatgaat gaactgactg tacaagagga acttcgaagc tatgaattgc cggaacagca
    28261 agaaaaagaa atcaaggtag tcagtcaatc agaatttgat caactgaact tgccacaatc
    28321 cactttacag ctagttcagg taaaagactt ccaagcaaac ctgaaaccaa tcgaaacact
    28381 tgttgctcaa aataaaatga acaatccatc tttgcaaaaa gtagagtaca gcaaccagaa
    28441 atatgaaatg tatgaagtgt ataatggtct attctctggt tttgaattta tgggattctt
    28501 cctaggttta gctttcttga ccatgcttgc tagctgtttg atgttcaaga tcttgtcagg
    28561 aagcaagagt gacattgctc gttatgaaat gcttgaaaag ataggaacac gacgcgggtt
    28621 gcttaaacaa tcgatccgta gagaagtagg cgtgttattc ttagcaccag gaatcttagg
    28681 tgtagtccat gtcttattcg gattacaaat gtttggtcta ttcatgcaaa atccatacaa
    28741 agatatttgg gttccgttta cgatattctt cgtattgtat ttcatctact atgtactgac
    28801 gacatggtta tatactggaa tcgtattgaa aaagagattg tgaaaaagct gtcctgcatc
    28861 aagtaataag aacagccaag caaaaatagc tcctcatatt tttcgtttgg ttgggcttat
    28921 taccgcagat gcaagctttt gaacaccgtt tgttcggaaa aagaggttgt gacaatattt
    28981 ttgtcacaac ctttttttca cggtctttta ttagaatata gtaaattttt tctcgttact
    29041 cacggaataa gctgttaaga aaacggtttc catgtatact ggaatcagtg caaatgaaag
    29101 aaaagagggt gcaaggaaca tggaaatcaa tgaacaaaat tttggcgaaa actatcactt
    29161 gattacgatc acgaatcaaa atgggacatc tttgtctgtc agtgatcttg gggcacgggt
    29221 tgttcatttt caaacaataa tcgataatga aaaacgagag ttgatcttag gttttgatga
    29281 agcaaaagaa tatctagaaa aagaccctta tatcggtgct tcaatcggac gtatggctgg
    29341 acggattacg aatggcacat tcaaattaga tgatgaaacg ttcgaagtaa aaacagaccc
    29401 ggacactggt cattgtttac atggtggtgc acctagcttt gaagccaagc agtggcatta
    29461 tacagtcatc aatggtgaaa acgaagcatc ggtcattttc tatcagacta gcccagatat
    29521 ggaaaacgga tttcctggaa ctttagatgt agaagtacgg tatacgctga cgaacgatga
    29581 tacatggcga gtgacggtcc aagggatcag tgaccgcaaa acagtattca atcctacaaa
    29641 tcatgtctat ttcaatttaa ccggtgatcc agcacagtca atcgacgaac atatactttg
    29701 gttgaatagc cgattatttg ctacgctcaa taaagataca acgcctacag gagaagtagt
    29761 ttcagtaagt ggcacagcat ttgacttcca acgtcctaaa aaattaggtg aggtctttgc
    29821 cagtgatttt cgccaatcaa tgatagtggg cggcatcgat catccatttt tgttgaaaaa
    29881 acaatctggt ttactaaatg ctgcggaatt gacaagccca gatgaaaaag tatcgatcca
    29941 agtaaaaaca gatgcttcag gaatcgtgat ttttacagca aattttggag aagatggacc
    30001 ggaaatgcgc gggaataaat tagtaaatca tggcggtatc acatttgaga ctcaagaact
    30061 gccaggagcg gagcgacttg aaagttttgg agatgtcact ctggaaccta accaagtacg
    30121 cgagtttgta acggaataca aaataaatat aaagaaatag aataaattaa aaagggagtg
    30181 tgacagtgtc acatcccctt tttaatttat tctatttctt taaaggcaga tcaagccaga
    30241 atgatttgtc taaacttaac aaggttagta caggaacagt tgcagcttta actgtacgag
    30301 tagttgtttc tggtatctgt cttgccgtaa aattgttcca caaatcccag attgaagcga
    30361 cacggaagat ggaagaagcg ttgttgacgc gttttatcgt tgaaaattct aatcctgtga
    30421 ttggcacgcc atctgttgtc catttgattg gcacatcgaa tcgcttagac atattgagga
    30481 tttcagcgtt tgcttcttca gttgttgctg cttctttttt cggttcatca tattttgtca
    30541 ggtcgtacgc ttcgatgatc gcgttcaatt tttgtgaata ttggcgatct gtagcataac
    30601 gtccttccaa atatttggtt gcttctttgt agtcttttgt ttccgacttc caagttggtt
    30661 tgtagaaatc aggattgcca gtcagtccat ttttgatcaa gtcggcatag tcttccaaac
    30721 tttccttgta agaaggatat ttacggaatt gtgcgcggat ttggtagctt tgtccggaac
    30781 tatcttgttc aagtgtattg aaggtcacgc tgcttccttt atatgatcct ttgataccaa
    30841 aaaggttatg atttggttcg cttgataata agctgtttcc agatccactt tcaaggatag
    30901 cttgagccat catgactgat gcgtagattc cttctttttg cccgatttcc tgtgcatcat
    30961 ctgcaatctt tttcaagaac tgatcagttg tttggttttg actgaaatgg aacgagccat
    31021 ctaatccatc taaaatagat gagccgcctg tcagcgaacc tgaagcagca ggtaaacctg
    31081 ctaagtttac tcggacagca aaatcaggcg tgaaatctga aatagaagtt actcgaactt
    31141 tatctcctgg ttgaggcgca tggataaatt gtccgttccc aatatagata cctacatggt
    31201 gagtaacacc ggaagaaggt ccccagaaga cgaggtcgcc aggttgggcc tgacttactg
    31261 gaatctgcgt acctgcatat tgctgttcgc ctgtccagcc accgatataa tgaccggttg
    31321 ctttcatata tacgtaataa gtcaaaccag aacagtcaaa agtatttgga ccttttgctc
    31381 cccatacata tggttttcca agatgcttga atgcttctcg aacgattgct tgttggacat
    31441 tcgttgtatt tgtaggcaat acagcatcaa ttggtgattt tacaacaaaa tcagaagaag
    31501 cagaagatgg attctctata ccgctttgat ttgctgtatt tggacgagat gaagcattgt
    31561 tcgttccgcc attcgatgaa ccattgtttg gatggtttgg ttctctctga gtatttccat
    31621 tgttattcga tccagatggg tttgtttgat tcgaagaatt cccgctgttc gaattattgt
    31681 tattattgtt tgaattattt gaaccattat tattgttgtt attacccaca tctggtttgt
    31741 ttggcgtttc cggatctggt gtactaggga cttcttcact aggatcagtt ggtttctctg
    31801 ttgaagtatc cggtccagct ttaaattctg cagtaataga taaattgttc gtacctactg
    31861 taaaggtctt tgcatcataa tttacttctt tggcaccatt aacagcgact gaagcaattt
    31921 gttcaccttc agctggcgta atagtgtaag ttaccttcgt ttccgctaat aaaccttcta
    31981 ccgttccact ttccgtaata tccaaaggtt gctgttcagg ggaatccgct gtcgaagcag
    32041 tcagttggat cttaccaatc aaagaaggag caattgtaag ggaatatgtc tcggctggct
    32101 tttcattttc tgatggatca gccggatcaa tttcagttgg ctcggtcgat cctgttgttg
    32161 agttagttgt tccagttggg tcttctgtaa aagaatccgt tgtttcacta gtagctgagc
    32221 tggatgtagt cgtatctgtt gtgactgttt gatcagctat ttgattatct acggcaaata
    32281 cggtattggt cgacaaaaca gacggagcaa tcagccaagt agcagctagc aaacgtatcg
    32341 tttttttatt tttcataata aactagtcct tttcctttca ttgattttct gtcttattca
    32401 gcattcatta gacatttttt ggaacagata atcgtgtcga aaagcttttt cttttcataa
    32461 atctgtctgt gtattctaaa taaaacaatt tgtaaataag caacgagcat tcattcgttt
    32521 ttctttgacg ttcattttat tgcatagacc acctttgttt ggggataaac aagtggggat
    32581 aaaaatggtt atgcatatgt aaattatacc aaagtttgga aagtatcaca aaaatttata
    32641 aaaataatga tcctaaaaag aaaaagactg aaatatgtga taaacatctc atacttcagt
    32701 ctttttcatt aactattcat aagacattag gtcttcatca aacccgctaa cgagagtagt
    32761 gatcattgct acgactagac taacagccag tcctgctaag taccagaaaa aagtaccgga
    32821 aatgaatgca ggcaatgtgg tcagactacc aaatacaaaa gcgatcgctt tcacttgggc
    32881 aaatcccatt acagctccac cagcagctgc accaatgcaa gcagcaacaa atggtttttt
    32941 caatgggatc gcaattccgt aaacagttgg ttcggtcact cccgcaagta aaatcgaagc
    33001 aaaagcagaa ccggagatcg aacgggtttt tgaacgttta cttcgcagaa atacacctaa
    33061 agctgcacca gccattccaa aattggctgc tcctgtcaac ggaacgattc ggtcgaaccc
    33121 ataagcagag atattactga tcatgacagg gtttacagcc cattggatac caaatattac
    33181 caaaatattc caaccaccag cgatgattgc gccactgatc ggtccgttaa tatccatcat
    33241 ccaagtaatt gcggcagcta aaccttcgcc aaggtaaaca cccgcaggcc cgaataccat
    33301 taacgtcaaa ggaaccgtga taactaatga aagtaaaggt acaaatacta attgcatatt
    33361 ttcgcgaata tattttttga ggaatttttc aacaaaagaa taaaagaaaa tacctaaaat
    33421 cgccggcatg acagtagacg tatactgcat cagtactaca ggaatcccca gaaaatcagt
    33481 agctgctttg ccactgtctg aaagtaaaga ggtcagattc ggttcaatca gtgctgcagc
    33541 aatgacagcc gcaatatatc cattcacttt aaacgttttg gcagcagaaa aagcaagaaa
    33601 gattggcaga aaataaaaga cggcattcga cgcagcagac aaaatatgat aagttccgct
    33661 tgtttcacta agccagccaa tcgtagtcgc taaaacaacc agacctttta atataccaga
    33721 accggcaaac agcggaatga tcggcgtaaa gatttccgat atcaaaccaa atagtttttc
    33781 gatcggattt agcttttctt tcggagctga atgatcgatt tgacgcgtca attccttgta
    33841 agcaggagcc aatccctgat ccatgattat ttctgcatat ctatcgagta aaatgaaata
    33901 cttaatggaa gggatttgct gttttaattt ttctgcattg gctactggct gttttttcaa
    33961 tttgatacga agccgagtcg cacatggaag gacagaatca atattgtctt ttcctcccaa
    34021 ctgttcaatc agcatatgaa tctgctcgga cagtaccatc tgttcaggat tcattgtcgt
    34081 ttccattaga agaccactcc actcactaat ataatattgg cgtaaggtgt catttcgcct
    34141 gtacgaataa atgcatgaca gttattcaac tcttttttca tttcttcatg tgggataaag
    34201 ctaatcggtg tatctggtaa caataatttg attttttcca atatttccgg gttcattgtc
    34261 tttatttctt ccgccaattc aatcgattgt acctcaagtt cttctaaaac gttttctaaa
    34321 acagataaaa aacttggaag accgtttgag acagccaaat ctattttttc agtaccaaag
    34381 gggacaggca tgcctgcatc cccgatactt aatttatcaa agtgccccat ttgtgcgatt
    34441 actcgtgaaa tatctgaatt gattactttt ccttttttca tgtttgtttc ttcctctcat
    34501 tgttaatttt agactgctga ttgttctaaa gcttcgcggt aagggataga aggttgcgca
    34561 ccataagact gcacagcaac agaagaggct agatttccat aaaggatcgc ttctttcaag
    34621 ttactaaggt ctggctgaag tgtgctgctt aatgcgccaa taaatgtatc acctgcggct
    34681 gttgtgtcaa cggcatccac tttaaaagca gggacgatac cgtgttcttt ttctgtatga
    34741 tagaaagcgc cggcactgcc tagtgtaatg attactgttc caatacctaa ttcatggagt
    34801 ttttcggctg ctgcgaccaa actgttatga tctgtcactc tgattcctgt aatgatttct
    34861 gtttctgttt catttggtat aatgatatct gtcttttcaa gtagttcagt aggaatcgtt
    34921 tcacgagctg gtgcaggatt caggatcgtt gtttttcctg cgtcttttgc aatagaaaaa
    34981 gctaggatcg tcgcatctaa gctactttca aactgagcaa taataaagtc actgtttgta
    35041 atcagttgct tgttttttag gacttcttgt tcatggaaag cattatttgc ccctgcatgg
    35101 atcaagatac tattttctcc tgctgcgtct accataatca ttgcttgtcc ggttgtggcg
    35161 ccttggattt cagctatccc agatgtatcg atgttttctt ttgtcagtag atctaataac
    35221 tgttgtcctt cgctgtcagc cccaacgcct ccaataaaag atgtccgtgc gcctgaacgt
    35281 ttggctgcca ctgcttggtt ggcaccttta cctccgccag cgtgaaaaat ctcatgcgca
    35341 tgcatcgttt cgcctggctt gggcatgttg gttaaacgaa gggtagtgtc catattaata
    35401 cttccaatta ctgtaaccgt gttcatttta caaacctcca actatctcat ttattttgat
    35461 gggaaaatta ttttttaaaa cgttttagta aaacgtttta atagttcgat aaaaatatag
    35521 cacaccttga aagctatttc aagggtgcag ttgaaaatct tttttctaat cgtacaggta
    35581 atcttttttc ttcccattct ttttctggga attggatccg atctaacaat aacttggcag
    35641 atgtttgtcc caattcgaag attggttgtg cgatcgttgt cagtttcggc ttgatatact
    35701 cgcacatatc tatattatca tagccaataa tgctgtagtc ttcagggatg ctttttccag
    35761 cttcctctaa tccgcgatat aatccaaaag ctaattcatc atttaaagca aacacaccag
    35821 ttgcagcaga atcaagaagt tctgctgtga tctggtagcc accttgtttt gagaactgtg
    35881 taggtaagag gatcagttgg tcgtgaggga tttggtaaac atcaagagca gatttaaacc
    35941 cctcaatccg agcgtggaca ttttccggtg gattttcagg atagacaagg gcgatcgttt
    36001 gatgtcctag actaagcaag tgcatgcccg ctaggtaacc accgcgaaaa tcgtctgttc
    36061 gtacggcatc actaaatcct tcagattttt tctgatccaa aacaatgaag ggccggcctt
    36121 gttttttcaa attttcgttg attgcatctg tagagacagc agaagtcgcg ataatgaatc
    36181 catccacacc tcgacgtgtg agctcggcta gatattcgat ttctttttga tgatcagaat
    36241 cagcattaca taatatcgtg acgaaattct gcttatacaa gatatcctca attcctcgca
    36301 tcagtgtact gaagaatgga ttcgtgatat caggaacgag tacaccaatt gttttcgttt
    36361 ccttggtaat catttgtcta gcaaaatagt caggctgata gcctaattct tctttggcag
    36421 ctaaaacttt ctgaacagtt ttcggactga atttctccgt atttccattt aatatcaagg
    36481 agacagttgt gattgaaacg ccggaaagtt tagcgacttc tttgattgtg atcttttttt
    36541 tcaccatgtt catactccta tctaaatcct gtggttattt tcccatgaaa tcaaaaagaa
    36601 gtaaatgaaa atcacgtgtg ccagtactta tttgctttgt tggatagctt cgattatttg
    36661 tttcgttgag cgtactcgtg cagttttcgg aaatatcttt tttatgaaca attgatggga
    36721 ttctttgtca cgatcactgc aagcatcttc agcaagcacg atatggtacc cgtgttgaaa
    36781 ggcatctaat gcagtagcat atacaccgtt tgttgtcgct acaccgctta aaatgatagt
    36841 ttcgattcca cgccggcgaa gttgaagatc aagagaagta ccaaaaaaag cacttggatt
    36901 gtattttgtg atagtcaaca tattatctgt atctttcaaa gaatccttca aaagtaaatc
    36961 agtgaattct ggaggtacag aagcagtcat tccacccata tcagtttgtt gactcaaata
    37021 agggaaagtg gagatgtcta cgttcactaa aactttcaag gcagcggtac gtttcaatgt
    37081 tcttactaac aaatcattgt ttgtcagaat agatgctgca gtgtgtggag agagtgtacc
    37141 gttatttacg atctcttttt gcatgtcgat cgcgacaaaa gcagtttttg ataaatcaaa
    37201 aatcatattt tcctttaaat cattcatgat gttccttctt tcaaataaca atgtttttta
    37261 tgtaagtaaa tcataaatcg tttatttgtc aactaaatta aaggattcct ttctttagtc
    37321 tagagaaatt cagtggtaaa ataaataagt gtgagaaaac gcattcgatg catagatata
    37381 gcgagaaata tcgtgaaaat ggaggaatca gagtgaagta tgtcgaagta aaaaatgaat
    37441 acaaacgata caaaatggga gaaacgacca ttacagcaaa tgacgatatt tctttttcaa
    37501 ttgaaaaagg cgaattagtg attatacttg gtccaagtgg agcaggaaag tcaacgattc
    37561 ttaatatttt aggtgggatg gatacacctg atgaaggtca gatcattatt gatgacacag
    37621 atattgcgca attttcagat aaacagctta ctgcttaccg acgaacagat gtagggttcg
    37681 ttttccaatt ctacaatctc gttcctaatc taacagcaaa agaaaatgtg gaattggcaa
    37741 cggaagtttc tcctgatgcg cttgatcctg tagaggtatt gaggcaagtg ggcctagctc
    37801 atcgattgaa taactttccg tcccaattat caggaggcga acagcagagg gtttcaattg
    37861 ctcgtgcact agctaaaaat cccaaacttc ttttatgtga tgaaccgaca ggcgcgctgg
    37921 attttgaaac gggcaagcaa gtattgaaat tattgcaaaa cgctagcaga caacacggca
    37981 ataccgtttt gatcattaca cataattctg ctttagcgcc catcgctgat agagtcattc
    38041 acattaatga tgctaaagta aggtctgttg aattaaatga tcatccttca tccattgatg
    38101 agattgtatg gtagggggat tttatgaaaa caagaacata tcttaaagca ggttttcgcg
    38161 aaattcggca atctaaagga cgattcattg caatcatcct tattgtcatg ctaggaacac
    38221 ttttatttgt aggtgtcaaa actgctggac ctgtcatgca aaaaacgatg gatcactatg
    38281 ttggtacagc aggactttct gatttgcaaa tcgtttctac tggtggcttg acacaaaaag
    38341 atattgctca agcagaaaag atcccagatg caaaagtaga aacaggaaaa cagatctatt
    38401 attccaatac tggcaaaaac gaagtgattc aaatattttc ttataacaaa aataacaagc
    38461 agaacaagct acaattaact gatggaaaac tcccggaaaa accaaaccag cttgtattgg
    38521 atgaaaaagc ggaagatgaa gggtataaaa tcggtgatat ttatcagatt gattcagatg
    38581 aattgaaaga aaaagagtat accatcacgg gctttgtccg ttctccattg ttcatcaaca
    38641 atttggagag aggatacgcg aacgtcggaa atggtagcgt cgattatttt gtctatttac
    38701 ctgaatccgt ttttaaaaca gatatttatt ctgtcttata tcttgatttt acaaacgtaa
    38761 agaatttgaa tacgtacagc aaagcttaca aagataaaat ggagcaaaat caagaaaagg
    38821 cagaaaagta tttacaagat cgacccgaag aacgcttaga agagctgaaa aaatcggcca
    38881 acgaagaatt agaaccggca cagcaaaaaa tagcggatgg caaagcacaa acaacgcaag
    38941 caagaacacg gttagaagat gctaaaaaac agttagcgca acagtcagca actatccagc
    39001 agcttcctga agcgcaacga acaatcgctg ttcaaacatt atctcaacaa gaagctcaac
    39061 ttgctgaaca agaaaggcaa ttagttgaga aagaacgtga actggcctct gcagaggaag
    39121 aattgaatcg aaatgcagaa aaaattgaaa atttaactaa accatcatac ctatacaatg
    39181 aacgttcaga aaatgtcggt tttcaagaat ttggtgatct gtctgatcgt atcgctgcga
    39241 ttgccaatgt ttttcctgtt ttctttttct ttattgccgc attgatcacc tttacgacaa
    39301 tgacacgtat ggtagaagaa aaccgcaggg aaatcgggac attaaaggcg ttaggttata
    39361 cgaaagttga aatcgcaaaa aaatatgcaa tctatgcaag tctagcctca gggataggta
    39421 ttgttttagg aacgatatta ggaacgaatc ttttgccaag gatcattttt gagctgtcta
    39481 acgagcggta tgatattggg agtgcgctta ttttttatga ctggccgccg attttacaag
    39541 cagctgccgc attcttcatt gccgcatttg gcgctgcaat gatcgtttta ttcaaagatt
    39601 taagagaacg gcctgctgca ttgcttcaac cgaaagcacc gaagccaggg aaacgcattt
    39661 ttcttgaata catcactccg ctgtggtcac gccttagctt taatcaaaaa atcagttacc
    39721 gaaatctctt ccgatataag tcaagaatgc ttatggcgat tatcggaatt gctggatgtg
    39781 ccgggctgat ggttgcaggc gtgggattga aagattctct aagttctgtt tcagccaaac
    39841 aatttggacc gatcattgac tatcaagcaa tcgttacatt ggattcatcc acggaagata
    39901 gtcgtcaaaa agcagcggat atgctgcaga agcaagaaaa agtaaatgag atcctagcta
    39961 ttcatactca aaccgtagaa ttgagaaaaa aaggacatgc ttcgcaaagt ctgacagtga
    40021 tcgtaccaag taaaacagct gatttggatc gttttatcca tttaactgat cttgaagaga
    40081 acccacgctc cttgcctaaa acaggaatcg ctattacgca aaaagtagca gaactatttg
    40141 acttatctac aggagatcat atttcgcttt atgatgaaga tcaaaaagaa tggaaagtcg
    40201 aagtaaaaga aatcgtgaaa aactatctag gtagctttat ttatatgtcc cctgaatatt
    40261 atgcttcagt tactgatcaa tcgatgatgg ataatgcatt tttgctgcgt acagataaga
    40321 tgacaacaaa agaagaagaa atgctttccg agaaactgct tgccacagac gctgtgacaa
    40381 atacgtcttt catgtccaaa caaatcgaaa cgcaagaaga atcaatggcg aacttagact
    40441 cggttgtctt aatctttgtc gtattatcag gactgctggc ttttatcgtc ttgtataatc
    40501 taactaatat caatatttcc gagcgtgtac gcgaattatc tacaataaaa gtccttggat
    40561 tttttgataa agaagtcacg atgtacattg ttcgagaaaa tatcatcttt actcttttag
    40621 gaatcatcgg aggatttggt gtaggatata tattgactga cttcatcttg caacaagcgt
    40681 ctatggagaa tgtcatcttt ccattagtga tcacatggtg ggcttatgtc ttatcagcag
    40741 gattaactat cgtatttacg gttattgtga tgattgttac ccatttcaaa ttaaagcata
    40801 tcgatatgat tgatgcgttg aaatcgaatg aataaaaaca gaggttgtga aatcagcggt
    40861 ttgacctgag tattagagaa acaaacggca aaatagtttt tcatattttg ccgttttttg
    40921 agctaatacc gaaggacagc tgattgaaca ccatgaatca gaggttgtaa aaaagccgtc
    40981 ctgcatcgag caaaaagaat actcaaataa aattagctcc tcatgtttta catttgagaa
    41041 gactgattgc cgtagataaa agcttttgaa caccatttat agaagaaaca agaatatttt
    41101 gctattttaa acaagaggat agacaaatac atgtctgatc tcttgttttt tattgggcaa
    41161 cagacctctt agaatgtata aagataaatt ttacaaacgc tttcggttca gttataataa
    41221 taaatgataa cttgtatata taagttgtgg taggtaataa atcagttgaa tggaggtaaa
    41281 tatccatgac atttaaagac aaagtcattt accaaatcta tcccaaatct tacaaagaca
    41341 gtaatggtga tggaattgga gatttaaaag gaatcaaaga aaaactcccg tatctgaatg
    41401 atctaggcat cgacatgatt tggctaaacc ctatttatcc aagccctcaa aaagacaacg
    41461 gttatgatat ctctgattac acagccatcg atcctatctt tggtacgatg gaggaattcg
    41521 aacagctaat agccgaagca aagaaatacc ggatagaaat catgctcgat atggtgctga
    41581 atcacgtttc gacagaacat gaatggttca aaaaagcact agcaggggac cagcattatc
    41641 aggacttctt cattttaaga gaagagccaa cggattgggt atcaaaattt ggtggaagtg
    41701 cttggtcgcc atttggagat acggggaaat attatttgca cttatatgat cggacacaag
    41761 ctgacttgaa ctggcggaac caagcagtaa gagaggaact attcaaagtt gttcgttttt
    41821 ggatggaaaa aggggtaaaa ggattccgtt tcgatgttat caatgttatc ggtaaagacg
    41881 aacaattgaa aaataataaa gaaaatgatg gcaaacctga atatacggac aaaccaatca
    41941 cccatacgta tctaaaagaa ttgaaccaag caacgtttgg gacggattcc actatcatca
    42001 cagtcggtga aatgagctct acgacgatcg aaaattgtat cttgtataca gaacctagta
    42061 gagaagaatt atcaatggtc tttaatttcc atcatttgaa agtcgattat gaaaatggga
    42121 ataaatggtc tactcctcca tttgattttg cagaattaaa gcgactattt catacatggg
    42181 gagaaaagat gagtgagggt aacggctgga atgctctatt ttttaataat catgatcagc
    42241 cacgtgcgct caatcgattt gtagctatcg atcagtatcg aaaacaagga gcagagatgc
    42301 tagcaaccat gatccatttg aaccgtggga cgccctatat ttatatggga gaagaaatcg
    42361 gcatgatcga cccagactat tcatcgattt ccgactatat tgacgtagaa agtttgaatg
    42421 cttataagtt actccttgaa caaggattca cagaaaaaga agcgtttcgt cggatcaaag
    42481 caaaatctcg cgataactct cgaacaccca tgcaatggtc agacacagaa caagcaggat
    42541 ttactacagg gaaaccttgg ttaaaagttg gaggcaagct agaagagatt aatgtagaaa
    42601 aagaactatc ttctgaagat tctattttgt cctattacaa gaaattgatt cggctgagaa
    42661 aaacatatcc aatcgttgca caaggagatt atcatgctta tgaagcaaat catccggaag
    42721 tttatggctt tcttcgccaa tttgaaggac agcagctgct tgtattaaca aatttctatg
    42781 ataaagatac aaggatcgcc atcccagaag gattcacaga agccaatatt cttatcgata
    42841 attattccga tactgttctt catcaaagtc tggagctaaa accataccag gcaattgctt
    42901 tgctgaaaaa ttaaaacaaa aacacaaaac gtcgttccta cgagctattc tcgtaagatc
    42961 gacgttttgt gttttattta aaataatctt cttcagaaaa tgaagagttt tcatactctc
    43021 ctttatcgcc ataaaattta ttgtagcgtt gtttgaattt acggaaaata aaagtaacga
    43081 gtaatttgac taacgtatag ataggaatac caaaaataac gcccacaatt cccattagat
    43141 cacccatgac aagtaaaacg attaagatcg taatcggatg gatctgtaaa cgatcaccca
    43201 ttactcgagg aatcaccaag tcaccgtgga gaagttgtac ggcaaaccag acaatgacaa
    43261 atttgacagc cataaaagta gagtcttgga atgcaatgat caaacctgga aagaaggcga
    43321 taaacggccc aatatatgga atgatacata acaacccagc agaaatagct aaaacactag
    43381 caaattctaa tccgatcagt aagaaacaaa cccagtagac tgctccaagg ataaatgaag
    43441 caatgatttg cccctttaga aatgaaccca attgttgatt agcaatcttt gtcaaatttt
    43501 taaaatctgt acgaaatgct ggtgggacaa tcgctaaaac tgactgataa aatttttgag
    43561 gatctctcaa aaggaaaaaa gcgacaattg gaccagtaaa taaagtaata aaaatagctg
    43621 taacagttga aaaaatgttg ctgatccgtt gtgcgccact ttcccaataa tttccaataa
    43681 atccagctat atcagacgtc acgtcgtcaa tagaagcgaa aaattgatta aatgaatcgg
    43741 caaaaggtgt tttctccaaa aattgtcgga gactctcttg aaaatcttga aacaaatctg
    43801 gcaaactact gatcaatgtc tttgcctgtt cttcaagaaa aggaataagc catatgcttc
    43861 caagccccaa taaaaataaa ataatcacat aaacgataat gactgacaag ttcctcgaaa
    43921 ggtgcttgct caaacgtttg atgactggtt ccaaaagata atacaagatc agtgcaaaca
    43981 ataatggggg caagatagta gcgataatga ttccgacagg cttgaatata aacgagatct
    44041 gatggaaaaa ataaatgatc agcgcaagta agataactat tcctaaaaca tagtaagagg
    44101 tttttcctcc taaaaagcgg atcaaacgat tcgttgtatc tgtttctttt ttcactcttt
    44161 ttcctccttt atctataaat tcattataga caaaagcggg tataaaagga aaatgataga
    44221 ggttgattgc aaaaatatcg tcaatcattc atataaactt tctcatcaat taaaataaga
    44281 aaacgcttga cagttctttt aaccatatga taaattaaat cctgttgcta aaacgtaact
    44341 attacggttt gcgaaaagcg aatcggaaca ttcaaatgaa agaagatgag aagaggagtg
    44401 tcataaatga aacgattgat aggtatattg gccatgctgg ttttagcagg tatgttaact
    44461 gcttgcgggg caagtgggaa agccgaggac agtaaagaaa aattgagcgt gatgacgacc
    44521 ttttatccca tgtatgattt tacaaaagca atcgtcgggg atgaaggaga agtagagtta
    44581 ttgattccag cagggactga ttcccacgat tatgaacctt ctgctaaaga catggcaaag
    44641 atccaagata cagatatctt tgtttataat gatgaaaaca tggaaacatg ggtccccgca
    44701 atccaaaaaa cattacaaga aggcaatgtc catacgataa aagcgacaga aggtatgctt
    44761 ttacttccgg gaagtgaaga aggacatgat catgaccatg aacacgggga agaaggccat
    44821 acacatgagc tagacccaca tgtatggcta gctccaagtt tagcaatcaa gcaggtagca
    44881 aacatacgtg atcagctgat tgaagcttac ccagaaaaac aggaagtatg gacaaaaaat
    44941 gcagcagcct ataccgagaa acttcaagca ctccatcagc tttatcaaga aacctttaag
    45001 caagcaaaac aaagatcttt tgtcacgcaa catgctgcat tcaactatct agcgttagaa
    45061 tatggattga atcaagtatc gattgctggt ctttcttcta gtgaagaacc gtctgcggcg
    45121 agaattgctg aactgaaaca ctttgttaaa gaacatggaa tcaactatat ttattttgaa
    45181 gaaaacgcca aagatagtat tgccagaacg ttagcaaacg aagcaggtgt atctttagaa
    45241 gtattgaatc ctttggaagg attgacgaat gaacaaatag aaaatggaga aaactatctt
    45301 tctatcatgg aagcaaacct cgaagcgttg aaaaaaacga cagagacaga aaacccgcta
    45361 gaagatactt tgacccccaa aaaagaaaag agcgtctaca acggttattt tgaagacgag
    45421 gatgtacaag atcgctctct ttcagattgg gcaggaactt ggcaaacagt agatacgtat
    45481 gtagaagatg ggacttttga tcctgttttt gaatataaag aaaaattgaa tcaggataag
    45541 acagcacaag aatataaaga atattatacg aagggctatc aaacagatat tgcttcaatc
    45601 aagatcagtg agaaagagat ggcatttacg tttaagaatg gaacaacgaa acgttcaaat
    45661 tatcgttatg tagggaaaga aatcttgacc tacgaggcag gaaaccgtgg cgtaaggtat
    45721 ctattcgaag cagaagataa ggaaagtggc gcttttagat atattcaatt cagtgatcac
    45781 acgattgcac cggctgactc cgaacatttt catttatatg caggaaatga aagtcaagaa
    45841 gtattgctga aagaaatgga gaattggcca acattttatc cagatacaat ggatggacgt
    45901 gaaattgcac aggaaatgct tttgcactga taggcaaaaa agaaccgaag ggtttggatc
    45961 ggctggtcat ttagtcggtc caaactattc ggttcttttt atgaaatcaa tttctttaat
    46021 ccacggatag aggctgggat ggtctcgccg tttgccaacg tgacttctct ggttttttta
    46081 ttaatcgacc gcacattttg tgtattgata acataggatt tatgacaacg gataaaattc
    46141 cgatgcattt tctcgatttc tttgatcttc tcatagaatt gtaattgggc atgagtcaaa
    46201 tggacttcca gtttatgtgg cgctcctagc gaagtagaaa aatattggat gtctttgatc
    46261 ggtaaaaatt tgatctcatc attaaactct attttgaaga tatctttttg gatttgggac
    46321 atttgaaaat cttcgaaaat tttgctcagg atatgctcga cattttcttt taaatcataa
    46381 agatcttctt tgacgatata atcagtcggt tcgatgttgc tttttaagat tttcatcgac
    46441 atttccgtat gggaagtcac atagattatt tttcctaaag gatctaattc acggatcaat
    46501 ttaccgatcg ctacaccatc catttcactg tcttctagtt cgatatctaa aaagtaaata
    46561 ccaaaagaag aagttctgat cttggctgca ttgagaagtt cttccggatc agaggtagaa
    46621 atatggacat aactgtctaa gttttcaatc atgattctat ttttgatcac cgttgataaa
    46681 acttctaatt gttttggatc gtcttcacaa atataaatgg gaatcatatc attactcctt
    46741 tattttattt gtatataaat cgtttgagaa aaacgctggt tttcaattga tgtttcaagg
    46801 aaaagataag gattggtctt tgtcaattca tccaagataa acaagccaag gccttcgttt
    46861 tttggatttg tttttgttga gaaccctttt tgtttcaact ggtttaacgg taatgtattt
    46921 gttgttgtat tgatcacacg aatcatcacg gaatgattct tcttgagtat ggtcaattcg
    46981 atttttttct tctttgcagc actagctcct tcaatcgcat tgtccaagat aataccaagc
    47041 atacgtacaa ggtttaccgt atgtttcgta tccagttcga ttggctcagg aaaatgaagc
    47101 tggacgatca accctttttc ctgtgccatc atgagtttta atgacagtaa actacggatc
    47161 tcaggaactt tcatattatc caacatactt agattggccg gaaaaaggtt catcatctgg
    47221 cgtgtaggta atatcgtttg atcataataa gaccgtaatc cttctaagtc gccttctgaa
    47281 atatagctgt gaagagaaaa aagcagattt tggtaatcat gtttgaattc tctcaagtct
    47341 tgatagtttt tttctagatc tttgatataa tcctgttgca tcctgacttg ttgctctaat
    47401 aactgatcag ctaatttctt attagagatt ttccaataat aaaccaagat gatcaggcca
    47461 acacacagat atagaatgaa aaaactcgta ttcaaagcta agacatcagg tgtttcacca
    47521 gaaaaacggg tataaaaaat aatgatatag tatgtaaata atatcaatgt cccgatcgta
    47581 ccgatcatac ggcggtccac agtgtgaaac caagtctcta acgatttttt taaacaaaaa
    47641 gctaataaag cagataaaaa agcggataag acaatgtgaa aatactgcaa tgaatctcct
    47701 ggttcaataa ccgaagattt caaaacaaaa aaatcgccga tgcttgttaa gtgatctcct
    47761 aaaagcgacg tcaataaccc aaaaggaagc gcgtaaacca attgttgacg cgaagtcgaa
    47821 aagaagaaaa aagtaagcat cagcattcct atagcaatta gagcagtaaa aatccctaaa
    47881 atcgaataga cccatccaat caaaatacaa gaaatcagaa tgactcccgc aaacagatat
    47941 ttgtatcgga tgtgatggtc aatcaataag atacaaattg tgtaaatcaa atattgcaac
    48001 gctatggacc ataaaaacat aaagtacagc tcccattgat ttttaccaaa ttatagcaca
    48061 attggcgtat tattttttta atttttcagg gattttgaat tcagaactca taccaaagca
    48121 gcagccagat acaaaaaagt cagataagtt agttaattgt ttaatgattg tttttttcat
    48181 gaaagattcc tccaaaattc atcaagtagt ttgtaatagg aagcaacgta gctatttcta
    48241 aaacaatccc aagtagcaaa taggtagaaa agtaagaagc tgagaaaaat aacgtaatga
    48301 gagaaaaaat tagaaacatg ctgatcgcct tctttaataa tgggggacgt ttctcgggtt
    48361 caatgggatt gattgctgta cctcttgatc catagcgaag cagtaacaaa aagcagctga
    48421 ttaacaaaaa gaccatcatt gggaaggaga tagtcaattg cagatgtttc atcaccaagg
    48481 gaacgcccca taaataaagt agtgtccaca gcagacaaac gaattcagag tcggaatgca
    48541 atccataagc atatctgcgg atgcagatat aagaaagaaa ggtgattcct gtttccgccc
    48601 atgagcctgt caccagagaa aagactagaa cgaccgctag ttttccaaga ttattcaata
    48661 aaacttctaa gacaaatttt acttggacat acaccatatc ttcttccgat ttgtctccgc
    48721 tgaataaggc ttctaataat ttgttcacaa ctttatcttt catctcatac gtacctccac
    48781 cacaaatttt atgctaaaaa aaataaaaaa cgtcttttgt ttcataaaaa gtggttttga
    48841 catcaaaacc gtcatgatct cctaaaaaaa tcttcgtttt ccaagaacct taccaaaaaa
    48901 agtgacagtt ttgatacgaa aagtactttt tatgacaagt ttgtgctttt tcgagtgatt
    48961 tatgttaaaa aattactgac cttataaaag gtgatggagg gattcttatg gaaaatgaac
    49021 aattgtttga agtcggcgaa acatatgcat ttaaatctga tttatttgaa tcaccaataa
    49081 aaggaaaaat agaaaaaatc tatgaaaata gtgtgatgat acgtgtcatg aaatgcgcag
    49141 tagaagacaa tgataccgct cataatctgc actggcagtt agttgtacgc aaatcaagta
    49201 caatagaaga ataaaaacag ataaaatgtt gagagcataa agaggttgtg agtctcccgt
    49261 ttagctctga gttacaagga ataatgttaa aaaacagctt ttcatgtttt atgaatattg
    49321 ccttgtgacc gaggtgttgg caggagaata ctgtttattt gaaaaaaggg ctgcgacaag
    49381 acttttgtca cagcctttat tttcatgaat aggagttact tttggctact tttagagaag
    49441 tagcttttaa ggagcagata aagtcaaggc ttttatttct ataaatgaag tagtaaaatg
    49501 aagaacagat tttattacag gaaggatgcc gataattgtg atcattgttg caggaacgat
    49561 cggtgcaggg aaaagcacac taactgaaat gcttgcccaa gatttagaaa caaaaccttt
    49621 ttatgagaac gtggaagata atgaagtatt gccgctattt tattctaatc cagaaaagta
    49681 tactttttta ttacagattt tctttttgaa caaacgcttt ttggcaatca aagatgcttt
    49741 cagccatgat gacaacgtac tagaccgctc catctacgaa gatagtatgc tgtttcattt
    49801 gaatgcagat ttagggcgtg tttctgaagt agaagtaaag cagtatgaag gtcttttaga
    49861 aacgatgctg aaggagttag aagaaatttc gccacaaaag aaaccagatc tactggttta
    49921 tattcgtgta tcctttgaaa ctatgttagc gagaatcaaa aaaagaggcc gggaatatga
    49981 acagctagaa caagatcctg aactctattc ctactataaa gaactcaatc gacgatacga
    50041 agagtggtat gaacagtttg atatatgtcc caaaatagtg atcgatgggg acaaatatga
    50101 tttcgtagcc gatccggcat gtggagaaca aatcgtacaa gaaatcaaga tgcgggcaaa
    50161 gaaaatgaca gaagaggcag atgcccagtc gatccgctac actacaaata acccgaaagc
    50221 aaagggaatc tttacaacta aataatataa aaactaagca aattgctcac tcgcagatat
    50281 ttctctgtga gcagcatctt gcttagtttt tttgattcat tgagatcaaa tagtgaaccg
    50341 attcgtatga taaaattgcc ataaatgaaa tgtctgagtg agggccatga tcaaaaataa
    50401 aatggaaacg atcaaaatcg aatgggcgcc aaacagatga tgacagaaaa caaggacaat
    50461 agctcctgcg ataaaggcaa gaacgacact tagatagtgg aaaaatcgat caaaatctgc
    50521 tttttcttta gaaatacaat aatcatataa gttcgttgct acccctttta aatttccagt
    50581 tgtaaataaa ttagtgtagg cacgcccgtt gattttatca aaagctaccc actggatcga
    50641 tgcagtaaaa gcaatcaaaa tcgttactaa tgcgggatgc tcatggacgg gtgtcaaact
    50701 aacaaagaaa aaaatcactg cttcataata tagaacaaac aagcgccaga aatgtttttc
    50761 tttttttcta aaataatcaa ttagatactt tgttagaaga atccctaaga aaaagaaaag
    50821 ggtagacaat acttttttac caacacttag ccattctccg tcaaatgctt ggatgatcga
    50881 tagaatgata ttgccagttt gagcagaggc aaaagcacca tattggatat aagtataact
    50941 gtccatggct cctccgataa atgtcagcaa aagtcctatt gtacgcccct cgtgaaagga
    51001 agtctgcatt tttttcatca ccttttatga atatttttgt cttccttatc ttagcataaa
    51061 cgattgaaaa aaaggaaaaa ttttcagaaa actaaaatca tatcgcttcc cattttcatt
    51121 tttagaaagg tctgttagta taaagaaaaa atagacaacg aaactttaaa taaccaactt
    51181 gaaataaaca aattcaaaga agaaagatgg gaaggaaatc aatcagttga atgaaaaaga
    51241 aattctccga ttacaaaaag aattgaaacg ctgggagaac aagtataaaa agcttcaaca
    51301 agtctttcgg ctacaagaag aaatcattgc tacctatgaa gcggcttatg gcgaagcacc
    51361 cggacttatt gaggaagaag tacaagtaaa agaaagtgaa ggaaacagct ctttctttaa
    51421 aatgatggag aaacgttcct ataatcgctt caatcaagaa caaaaagcag ctatcacata
    51481 tgatatgaag aaacatcttc gaatcatagc aggagctggg agcggcaaaa cgcaaacaat
    51541 ctgtgcaaaa gctgtttatc tgatgacgca aaaacaagta gacgaagaac gcatactaat
    51601 gatcactttt acaaggaacg ctgcaaatga actaaaaaaa cgagtagata atttcagcca
    51661 gagaaaaacc gctgtccata tcggaacatt ccatagtatc ttttttagga tatacaatga
    51721 aatttgtcga aaatttcctg aagtagcaat gcaaggaata caaggagact tttcaaagga
    51781 tactgcacaa aaagtgaatg ctgttttgca acagctgata cgtaaatata atctttatat
    51841 atttgatcaa tatggagaaa aaacgattgc ttctcggtta gactattggc agaatatgaa
    51901 cctttcagta gaagaaatgg ttcagttagt taaagaaaaa tatgattcca ttgataagaa
    51961 tgcacgacag ccgatatcag aacgactgta tggattgctc acggaactcc aagaacaaaa
    52021 aagaagccag caattgctag agttcaatga tgtactccaa aatctgaaaa gcgctttgga
    52081 gaatgaggag atccagcatt atatcggcca aaaatatgat tatctattca ttgatgaatt
    52141 tcaagatact aatccacttc aatgggaaat cgtgaaacgg atgacaaagg aaaacaggat
    52201 caagttgatt gttgttggag atgatgatca gagtatctat ggattcagag gatcagagcc
    52261 agcatacatc aagaactttg aaaaagaata tcctaccaaa actcttttct tattaactaa
    52321 ttatcgttct cgggcagcta ttgtccaagg agcaaataga ttgatcagct ataataaaaa
    52381 tgatcggata ccaaaggcga tgattccagc acaaaaagaa gcaggtgtta tggaagccta
    52441 tttgttttct gatactaaag cagaaagtca gtggctaatc aaacaaattc agacatttat
    52501 tcaaaaaaat gggaaatata aggaatcaat catattatac cgatcagctt cacaaacgca
    52561 gcagcttgtc caatctctgc tgaaaacgaa tattcctttt gtactggaag cagatagtcc
    52621 atatgaaggt atctttggta tcaagtcttt ccaacatttc tatcaaaaaa ttgttcattg
    52681 gcaaacagca ctgaatctgc aaaagaaaaa tcaggcctac cagcaattgc tccggcagct
    52741 gatggcagac tgctacctga aaaaaagtga atgcgatcaa tacttttctc ctaaatatca
    52801 aaatatagcc gttgcagatt atattttgtc gataagcccc aatttaaaat cgaaatctgc
    52861 agaaatccga gagtttgagg aagcgttgcg taaaacagac cagcatcaag aaatctattc
    52921 gcttgttcaa agctatcttc atctgccacg tatagctaag gaaatcgacc agagtgatca
    52981 agaatggata cgagaagaag taaagcagca tgctactttc agaagactcc aagccttatc
    53041 gcaagaaacc caagcgacag ctaaagaatt aaaaacccgc ttgattgctt acagacaagg
    53101 aaaacttgat gcactatgta tccagagcat tcacaaaagt aaaggtctta gttaccgaaa
    53161 tgtctttttg attggttgta acgaaggaag tttgccttat aacggagcga cagaaaagaa
    53221 agtaatcgac tttaatgaaa taaaagcaga accagtaacg acgatagaag aagaacgaag
    53281 actattttat gttgcgatga ctagagcaag aaatcgtctg tatctatgtg tacctcaaat
    53341 gaaaaattca agaaaattga aagtatcgag atttatcaaa gaaaccggga gtcgtgtgaa
    53401 aaaaggaaag tgatagaaga gagggtgtga cagaagtggt cagtttcaag aaagaagaag
    53461 gaatgtacga aaattgcttc tcaaattttt gtgaaatttc agcttatttt cgaagaagct
    53521 atttctggaa cacagtttat tcggaaaagg gaggtgtgac aagaatt
//
LOCUS       DACCXO010000008        51007 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.008, whole genome shotgun sequence.
ACCESSION   DACCXO010000008 DACCXO010000000
VERSION     DACCXO010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.008"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            117..962
                     /locus_tag="IVY70_000419"
     CDS             117..962
                     /locus_tag="IVY70_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7957580.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1243..2463
                     /locus_tag="IVY70_000420"
     CDS             1243..2463
                     /locus_tag="IVY70_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP7957581.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2460..5180
                     /locus_tag="IVY70_000421"
     CDS             2460..5180
                     /locus_tag="IVY70_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7957582.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5164..6108
                     /locus_tag="IVY70_000422"
     CDS             5164..6108
                     /locus_tag="IVY70_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7957583.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6177..7187)
                     /locus_tag="IVY70_000423"
     CDS             complement(6177..7187)
                     /locus_tag="IVY70_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7957584.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7367..7711)
                     /locus_tag="IVY70_000424"
     CDS             complement(7367..7711)
                     /locus_tag="IVY70_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP7957585.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7704..8138)
                     /locus_tag="IVY70_000425"
     CDS             complement(7704..8138)
                     /locus_tag="IVY70_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP7957586.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8276..9013
                     /locus_tag="IVY70_000426"
     CDS             8276..9013
                     /locus_tag="IVY70_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7957587.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9006..10217
                     /locus_tag="IVY70_000427"
     CDS             9006..10217
                     /locus_tag="IVY70_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7957588.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10424..11203
                     /locus_tag="IVY70_000428"
     CDS             10424..11203
                     /locus_tag="IVY70_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP7957589.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11273..11935
                     /gene="trmB"
                     /locus_tag="IVY70_000429"
     CDS             11273..11935
                     /gene="trmB"
                     /locus_tag="IVY70_000429"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP7957590.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12033..13787)
                     /gene="ade"
                     /locus_tag="IVY70_000430"
     CDS             complement(12033..13787)
                     /gene="ade"
                     /locus_tag="IVY70_000430"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP7957591.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13919..14848
                     /gene="cpaF"
                     /locus_tag="IVY70_000431"
     CDS             13919..14848
                     /gene="cpaF"
                     /locus_tag="IVY70_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP7957592.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14805..15854
                     /locus_tag="IVY70_000432"
     CDS             14805..15854
                     /locus_tag="IVY70_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP7957593.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15851..16129
                     /locus_tag="IVY70_000433"
     CDS             15851..16129
                     /locus_tag="IVY70_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP7957594.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16104..16574
                     /locus_tag="IVY70_000434"
     CDS             16104..16574
                     /locus_tag="IVY70_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP7957595.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16591..16893
                     /locus_tag="IVY70_000435"
     CDS             16591..16893
                     /locus_tag="IVY70_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957596.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            16910..17281
                     /locus_tag="IVY70_000436"
     CDS             16910..17281
                     /locus_tag="IVY70_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP7957597.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17253..17612
                     /locus_tag="IVY70_000437"
     CDS             17253..17612
                     /locus_tag="IVY70_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957598.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17784..18791
                     /locus_tag="IVY70_000438"
     CDS             17784..18791
                     /locus_tag="IVY70_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7957599.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18816..20000
                     /locus_tag="IVY70_000439"
     CDS             18816..20000
                     /locus_tag="IVY70_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP7957600.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20437..22020)
                     /locus_tag="IVY70_000440"
     CDS             complement(20437..22020)
                     /locus_tag="IVY70_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7957601.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22598..22741)
                     /locus_tag="IVY70_000441"
     CDS             complement(22598..22741)
                     /locus_tag="IVY70_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP7957602.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(22964..23443)
                     /locus_tag="IVY70_000442"
     CDS             complement(22964..23443)
                     /locus_tag="IVY70_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7957603.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23516..23800)
                     /locus_tag="IVY70_000443"
     CDS             complement(23516..23800)
                     /locus_tag="IVY70_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957604.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23878..24072)
                     /gene="ssrS"
                     /locus_tag="IVY70_000444"
     ncRNA           complement(23878..24072)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IVY70_000444"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24144..25430)
                     /locus_tag="IVY70_000445"
     CDS             complement(24144..25430)
                     /locus_tag="IVY70_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP7957605.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25568..26239
                     /locus_tag="IVY70_000446"
     CDS             25568..26239
                     /locus_tag="IVY70_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP7957606.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26205..26693
                     /locus_tag="IVY70_000447"
     CDS             26205..26693
                     /locus_tag="IVY70_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP7957607.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26706..27653
                     /gene="prmA"
                     /locus_tag="IVY70_000448"
     CDS             26706..27653
                     /gene="prmA"
                     /locus_tag="IVY70_000448"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP7957608.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27654..28418
                     /locus_tag="IVY70_000449"
     CDS             27654..28418
                     /locus_tag="IVY70_000449"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP7957609.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28651..30864
                     /locus_tag="IVY70_000450"
     CDS             28651..30864
                     /locus_tag="IVY70_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP7957610.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30875..31321
                     /locus_tag="IVY70_000451"
     CDS             30875..31321
                     /locus_tag="IVY70_000451"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP7957611.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31492..32751
                     /locus_tag="IVY70_000452"
     CDS             31492..32751
                     /locus_tag="IVY70_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP7957612.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32840..33787)
                     /locus_tag="IVY70_000453"
     CDS             complement(32840..33787)
                     /locus_tag="IVY70_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP7957613.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34181..35473
                     /locus_tag="IVY70_000454"
     CDS             34181..35473
                     /locus_tag="IVY70_000454"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP7957614.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35488..37257
                     /gene="aspS"
                     /locus_tag="IVY70_000455"
     CDS             35488..37257
                     /gene="aspS"
                     /locus_tag="IVY70_000455"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP7957615.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37366..38241
                     /locus_tag="IVY70_000456"
     CDS             37366..38241
                     /locus_tag="IVY70_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7957616.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38507..40390
                     /locus_tag="IVY70_000457"
     CDS             38507..40390
                     /locus_tag="IVY70_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP7957617.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40465..40749)
                     /locus_tag="IVY70_000458"
     CDS             complement(40465..40749)
                     /locus_tag="IVY70_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP7957618.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40739..41473)
                     /locus_tag="IVY70_000459"
     CDS             complement(40739..41473)
                     /locus_tag="IVY70_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP7957619.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41602..42225
                     /locus_tag="IVY70_000460"
     CDS             41602..42225
                     /locus_tag="IVY70_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP7957620.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42417..43538
                     /locus_tag="IVY70_000461"
     CDS             42417..43538
                     /locus_tag="IVY70_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP7957621.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43545..46673
                     /locus_tag="IVY70_000462"
     CDS             43545..46673
                     /locus_tag="IVY70_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP7957622.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46692..49886
                     /locus_tag="IVY70_000463"
     CDS             46692..49886
                     /locus_tag="IVY70_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7957623.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50032..50739)
                     /locus_tag="IVY70_000464"
     CDS             complement(50032..50739)
                     /locus_tag="IVY70_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP7957624.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16683 a         8927 c        10609 g        14788 t
ORIGIN      
        1 tttcctattc ttaaaatgat gtttgtactt acttaaatta ggtataatta gtatatgttt
       61 tgtgagaaat ttagacttac aataagcagt gaatttataa tcaacgtata ggagacatgg
      121 atgaaatgaa actaattggt atagatttag acggtactct tttgaattct gaccagagca
      181 ttagtcaaaa aaatgcaaga acaatgaaac atttaccgga agactgtttt ccattcattt
      241 gttctggaag agaagtagaa gacattaata atattctcaa aaaatctggt ttaaggatac
      301 cagcagtagg gttaaatggt tctgttggat ttgatggaga aaaaaagata tttgagtctt
      361 ccttcgataa aaatgatgta aaaaaaattg attctgtcct atcgaaattt ccaaccaaaa
      421 tatatacaaa tctcggaagt tatgaatctc agaattataa aaataaaatg aaaaaaattt
      481 ttcacgaggt agggagtgaa ttttctatca aagagctaaa ctatgagctt gagtatgaaa
      541 aatctatttg ttcaatccct tataataata ttgaggaaat tatagaaaag gatgctatca
      601 gcatttacaa attttttatt tttattccta atcgtcaatt gaaagctaat atatttaacc
      661 atttgaaaaa gatgctaaat gtgtctgtta ctgaatcggc acctgtaaat attgaaatcg
      721 tacctcaaaa tgtatctaaa ggtgtggtgt ttgatcgttt ggaatcaatc tacaatttaa
      781 agaaaccttt gagaatcgcg atcggagata gtttaaatga cttatctatg tttgaaagtg
      841 cagatattag ttttgcaatg ggaaatagtc atcaagaaat catgaaaaaa gctgattatg
      901 tcactgtttc aaatgataaa gacggagtat ctcaagcatt tgaaagaatt cttaccatat
      961 aaactaatta aggtagtata gaacatagaa ttctaaatac ggaaaaatgg agcataaatt
     1021 ccagtacaaa tagtacttct tcagtgtcaa acgaagcgaa tataacatag atatttagga
     1081 ttgtgacaaa aatcatgtca caatcttttt ttcgaataaa cttatttttt gaagtcggcc
     1141 atttatcgta atttctttgc ataatacgaa caaattttcg gttattgatg aaaaaaaaca
     1201 ttgaaaatgg tatgataaaa gaaaaaaagg aggaaatttt taatggtgaa atttatccat
     1261 gcggcagatt tacatatgga tcggtcattt gaaggactga caactcttga taaaactgta
     1321 caggaaaaac tattgaagac gaatctgacc gttttatcaa atatcattga acaggcaata
     1381 ataaataacg ttgattttgt tttacttgca ggagataatt ttcatcaaaa tcgtccttca
     1441 ttaaaaatac agaaacattt ttcagaacag atgaaacggt tagaaaagaa ccatatacct
     1501 gtatttataa tattcggaaa tcatgatttc tatcaaaaag agcggtattg gttctccttt
     1561 cctaaaaatg tccatttatt tacatccgaa acggtagaga caaaaaaaat aacgataaaa
     1621 agtggagaaa ctgtctctct cagtggattt agttatcgtc aaccgtggat tcaaaaagac
     1681 aaagtgatgg aatttccttc tagagaactc acagattacc atattgggtt gtaccacggg
     1741 gaacctggcg tctctcaaaa aggaaactat gctcctttcc aaccctcaaa gatgcaagaa
     1801 aagggatacg attattgggc tttagggcat atccatgtgc caacggtctt aaatgaaaag
     1861 caaacaattg tttatccagg tgcgcctcaa ggtcatacta aaaaggaaca agcttctaca
     1921 tctattctac tagttgaatt aagtaaagga agttgccaaa tacatccgat taaagtagca
     1981 gaggtctatt ggaagacaaa ggaaatatca ttgaggaaag caagaacgac aaaagaggtc
     2041 attgttcaca ttcgtcagca gctttcaaaa gtagaggatg gatttagttt aattgagata
     2101 aaattaaaag attatgacca tctcaataca gatgttttgg aacggataca atcaggagag
     2161 ctattagact atttgttaga ggaattctca gataggataa atgatttctt tatttggcgt
     2221 atttctctca tagagaatac tttttatacg aagaccccac ttgctgcttc tgctaaattg
     2281 atggaacagt tatttcaata ctatcagaca cctcaagatt tccaacaaat tttaaatgaa
     2341 gtatatagtc atcaagaagc ggctagaata ttgagcgaac tgccggaata tcaagaagaa
     2401 acacttgaaa aagccaagca gctgcttaat caagattttt tatttgagga ggatcaggaa
     2461 tgaagatttt aaggatcgag attgctgctt ttggaaaatg gcgtcaaaaa agttttgact
     2521 tttattcagg caatcagctg atctatgggg gaaacgaagc aggaaaatca acgatttatc
     2581 aatttattca agcaatatta tttggtttcc cttcaaaagg ccgtaaaaaa aaggattata
     2641 caccaaaaga cggctcagcc tatggcggca aaatatggct taaacatcct gtatatggtg
     2701 aatttgctgt cgagcgctac aagcagcaaa atcgcggaaa atcgaaggtg tggctggggg
     2761 atcaagtggg aagtgatgag ttactagaaa aactgatcgc acctttgacg aaagagttat
     2821 tccagcaggt atttactttc cagcaagagc agttgatgga actagatcat ttgagagaaa
     2881 aagagctaca tgatgcgctg atttctttag gcatcacggg cagttctcaa gtattccaaa
     2941 agcggcaaga atattatcag aaagcacaac aattattcaa gaaaaaaggc caaaaattac
     3001 taatcaatca aaaattaaat gaatggaaag agctgcaaga aaaaattcat caaaaacaag
     3061 aacaggaagc ttattttggc aagttgatcc aacaagaaca ggaatacgag tcgcaacagc
     3121 agcagttatt ggcacaatta aaagaggcga acaaacaatt ttcattagcc agacagcaag
     3181 aacttaattt ttctttatat gaagaatggc aatctctcaa aaaaaacagc actacaggtc
     3241 ttcctgatga gcaacttctt ttagatctgg agtctttctc caaacggtat cagcaaacaa
     3301 atgagcgttt ggaagaatta gaagcaacac tggaaaagca tagcgggatg gatcagcagt
     3361 cagccagata ttatttttat ttagagcagg agcaggaact aaaagatctc cagcaacttc
     3421 aatttacagc ggaacaatta aacgaagaat ggcaaagatt acaaattgag actagagaaa
     3481 caaattcaga attgaagatt ttggaacaaa ggtggcattg gcagaaaaat ccgttaccgg
     3541 aagaattttt atatgaagaa gaatggaagc atcttctggc tgaagaaaga aggattgctg
     3601 atctagaaag ccagatttct gtgcgtctcc aaatagctaa agaacagcaa gagatgattg
     3661 aagcagaagt gaatgacttg gaagagacct atccagcatt attacaaaat cagtcagaga
     3721 tagcaccagt ctcttcatct aaaccgtata tgctgatggc tggcggagca ataatcattg
     3781 caggtatatt tgtaccaatg cccttgaaag tgatcctttt actaattggt gcaggcatga
     3841 ttggctattt cttctacaaa aggcaaccaa aaattaaaac taaaaatgat ttctctgaga
     3901 taaaaggaat gtggcaagaa aaactaggac aacttgatct agccttagct caattagctg
     3961 aagcagaaga aaaacaaaag agaactggag aagaaaaaca gattcttcag caagaagtat
     4021 atcgttttgc ggatcatcgg cacttaggaa agatggatac gctcaaactc atgaaagaat
     4081 atggacagga agtcatagat tatcagaaat tatatggcgt atttgagcgg aaacataaaa
     4141 gagaacagga aatacaagaa aaactacgac aaatcgacca aaaatttgag tttgttaaag
     4201 aatggttacc gatacaagat aagacaattt cagaaaaaat ggtattgctt tcccgtttta
     4261 tacaagagat ggaacagatg aaatttgcca gaagctatca gcaaaatact ttattgaagc
     4321 aagaaatcaa tcaattgaaa aagaaacaaa aagtatggat acaagaatac agcgaacaat
     4381 tgactcaagc aggcttatct tatccttcgg aagtacctgt ctttttacaa aaagccaaac
     4441 aagccgaaca gcagatgaaa agatacaatg aattatcgga aatgcttact ccactatttc
     4501 ctgaagagct gacaaaagag aaattatctg taagactgcg ccaactgaaa gaaaagcaat
     4561 cccaactcca gcaagaagaa aaagaagcaa gtgaaaatcg gcaacgactc caacttcaag
     4621 tagaagagtt gcaaagagac ggcacattgg acgaacttta tcaacaagct agccggcaaa
     4681 aagcaaaatt acaggaatta ctggaggaat ggggagcact tgaaatcgcc gggacattct
     4741 taggtgatct ttcaacagag atgagtgaaa gacaattacc ccagttgtta agagcagcag
     4801 gtgattattt aggtatttta acaaaaggaa attatcagaa gatattactg aaaaatgata
     4861 cactgacttt aacagacgga tatacagaat ttcccatcta tgagttatct acaggtacga
     4921 aagatcagct gattatggcg atgagatttg cttacctctc attagaagct aaccgttcga
     4981 tatgcccgat catcattgat gatggctggc ttcattatga tcatcagcga aagtatcaat
     5041 tagctgaact tttagctaaa tttggagaaa agtatcaaat aatttgtttt tcttctgata
     5101 aagaaatggt aagctattac catgagatga accaaatcgt aaaacaatta gaaggagcaa
     5161 ataatgaaaa aaattcgtga tttagtaatc gatgagcagt ttgaacaatt tgtcctgatc
     5221 aaaagtgcag atgtaagaat cgctaaaaac ggaaaaaaat ttattgcttt tactttccaa
     5281 gatacttcag gtactatcga tggtaaatac tgggatgctt cggaggagga aatcaataaa
     5341 tttacagccg gcacagtcgt ttttctaaac ggtaaaagag aggtttatca aggaaaccct
     5401 caagtaaaga ttcttcatat gcgcttaaca agggtagatg agccaggaga gcctagttta
     5461 tacatggaaa gagccccttt gaagaaagaa gaaatggaag aagaaatcaa tcaattgttg
     5521 tttgagatca ccaatgcgca ttggaatcgt attgttcgct atctacttaa taaatatcaa
     5581 aaacaatttt ttgagtttcc ggcagcaaaa agaaaccatc atgcatttgc cagtggatta
     5641 gcttatcata ctttgacaat gcttcactta gggaaatcaa tcgccgatga atatcctgag
     5701 ctgaatcgag cattgttgta tgctggtatc attttgcatg atttaggaaa ggtgattgaa
     5761 ctttccggtc ctatgtcgac agaatatacg cttgcaggaa atttgatcgg tcatctggta
     5821 ttgattgacg aagaaataac taaagcctgt cttgcgttga aaatcagcga agctgacgaa
     5881 gatgttctcg tattacgtca tatggtttta gcacaccatg gcttattaga atatggctca
     5941 cctgtacgtc caagaataat ggaagcagaa atattacatc aaatcgacaa tatcgatgct
     6001 tcgatgcaga tgatgttgac ttcgattcgt caaacagaac caggaaaata caccgatcgg
     6061 attttcggta tggacaaccg aagtttctat gtaccaaaag atttatagca aaaatttatt
     6121 tttttcattc aaaacaaaaa aagcgagaca taagtcttcg ctttttttgt tttcttttat
     6181 tttgatgaat cagttgtttc ttcagttgaa gattcttttg ttgaatcagt tgattttgtg
     6241 tcagaactct ttgttgaagc tgtagtttca cttgagtctt tcgtacttga tgaagttgtt
     6301 gtgaatgcac tcaacacatt ttcaaaggca tcatctttga tttttacatt tgcatctttt
     6361 aattcttcac cgattacttt agttgtgaat gcattgtcac ttagtttagt ttcagtagcg
     6421 atatctttta gttgatcttt gtatttatcc atgtcattgc ctttattttg gtttttcacc
     6481 attttgacaa cgtagtattc tgtcgcataa gaagtaggat ttgttgtagt aataacatcg
     6541 gagatctcac cgtctttcaa tttgaaagct gcttctttta cttctgcagg aatagttgtt
     6601 gtcgttgagt cgaatttaac tttaccgcca tcttcttttg tctctgtatc agttgatttg
     6661 tctttggcta atttagagaa atcatctcca tcatccgctg attttttcac atcttttgct
     6721 tcatcttcac ttgataattt gatgatttgt gcttctactt ctggatggaa agatttccaa
     6781 gcagttttca aatcgtcatc tgtaatatct acgtgagctt tcaagccagc ttcaaaagct
     6841 aaattgttac gaatatattc tttataagta tctttcgtat atccagcagc ttctaattgt
     6901 gattcaaaag tatcacctaa tgatttcgct tgtttgtcgt attctgcatc cacttgctta
     6961 tcggttactt tatcgccata tttgttattg aatactttat aaatgatcat ccgttgtact
     7021 aaggattggt tcgatgattc aagttttgct tcatcataga aatcagatac agtgatcgtt
     7081 ccgcctttca tcgttgcgat atctttatta gtatcgcctg aacaagctgc tagagttaaa
     7141 acagccaacg cactagtagc tgccaaaata attgattttt ttttcattaa aatttgcact
     7201 cctgtaatta gttattattt ttatatatta actataccat atcccatttc ctttaaaaac
     7261 gttgtcgtgg tagtttcaaa caaaattcaa aatttcatca cattcctagg aaatacgcaa
     7321 acttttctca cttatatagt taaatctgtt gagaaaataa tcattattat atttgttcag
     7381 gatactctgg cagatcagct tttagttttt ctacttgttc attgatttgt gtgatacgtg
     7441 gttcggcttg gaatttaaaa tcttcgatat cttttttgat tgcttcttgg aaagcaggaa
     7501 tcacagattc agctgtttct tttgtttcga tcaatgcgct tttaaaatga ttcaggctgt
     7561 tatttaaatc atttgtcaga gttgttgctt cttccaattc ttcgatcagt ttctttcttg
     7621 tttcattccc gctcctgggc gccaataaca gacctccgac agtgccagca gcagctccaa
     7681 ataacagtcc tttgaaaaag tttttaatca tcgcaggaca cctgctttac gattgtttct
     7741 tgaatagctt ttagttcttc cgatccataa ctatcttgat gattgacaaa gtgaatagaa
     7801 aaatcatcct ctttgctgta gcgagggact aaatggatat gtgaatgaaa aacagattga
     7861 taggctagtt cacggttgtt attcaagata ttaagacctt tcatttctgg aaaagctttt
     7921 tccaatgctt gagcaatctt aggcacacgc gaaaaaacct ctcctgcaag ttcgggatca
     7981 tattcaaaaa tatctgctac atgacgttta ggcacaacta atgtgtgtcc tttcgttgct
     8041 tgagagatgt ctaaaaaagc atagaccttg tcgtcttcat atattttata gcttgggatt
     8101 tcttgattga tgattttgca aaaaatacag ttttccatat aaaacatcct ttctataaag
     8161 aataaatact tcagcttaac tttaccataa gttaaagcta ttgtaaggaa aaaaacaaaa
     8221 taccgtcatt ttcttcgtgg tataataaat aaaaataaat ggcggaggaa aaagaatgag
     8281 tttaattatc gatcatgtca caggggggta cggacatctc cctgtgttaa aagatatcag
     8341 tttcgatgta cagcctggag agatagtagg gttgattgga ctgaacggag cagggaaaag
     8401 tacgaccatc aagcacatta tcggtctgct ggaagcttca aaaggaacga tagaaatcaa
     8461 tcatctatcc ttaaagaaaa atacggaaga atatcgcaaa caaattgggt atattccaga
     8521 gactccttct ttatatgaag aattgacatt gagagaacat atcgaagtca ctgcgatggc
     8581 ctacgatata cctatggata tagcaatgaa aagagcagaa aagttgttgg agacttttcg
     8641 gttatctaat cgtcttgact ggttcccagc taatttttca aagggaatga aacagaaagt
     8701 gatgatctta tgcgcctttt tgatcgagcc ttctttatat atcatcgacg aaccattttt
     8761 aggcttggat ccactagcta tcaatgcatt gcttgaattg atggtagaaa tgaaaaaaga
     8821 aggagccggt attttgatgt ctacgcatat tttagctact gcagaaaaat attgtgatcg
     8881 ttttgtggtc ttacataacg gagagatacg agcagacggt tctcttgaga tgttgagaaa
     8941 agaatttcag ctgccggatt cttctttgga cgaaatctat attgctttaa caaaggagca
     9001 ggcacatgag tagttttttt caaaaacgtt tagcaagaca tcaaaagaaa atgatgaaat
     9061 atatgcgtta tgtattgaac gatcattttg ttttagtttg tcttttcttg attggcggtg
     9121 tgggattata ttattctaat tggttgaaaa ccctgcagcc accgtttgca attggagggc
     9181 tcataatctc tgtattttgg atgctctgtt tattcgtggg taagatggct acttttgcag
     9241 aggcagcaga tattgttttt ttacttccta aagaaaaaga aatgcaaacc tatttggaac
     9301 gagcttttcg ctattcttgc atttttccgt tgatcttttt gatattggca tgcggattgg
     9361 ccatgccatt agtggtggtt agtacggagc aatccttttc gatgtttttc gtctatctga
     9421 tcatattatg gagtttgaaa ttcagtcatc tgcaaatcaa gagagcagaa ttgtttcgac
     9481 tagatcaaca agttattcgt tattggaaat taggctggtt tttttcttct ctgatctttt
     9541 tgtttctatc attttattgg ctaatatggg caggaatagc aggtgcatta gtacaggcaa
     9601 gtctatatta ctggtttttg tggaagcaac aagaaaaagg attggattgg gagaaaatgg
     9661 tgcatgtaga agaacatagg ttgcatcaga tctatcaatt tatcaattta ttcacagatg
     9721 ttcctgaaat tacggcaaag atcaaaagaa gacgctattt ggatcctata ttgactcata
     9781 ttaaaagaga atcaaaaaac acttatcttt acttgtttgc aagaagattg gcaagaggtt
     9841 ctgattttag tggtctttat ttccgattag taattatagg tgggttattg attgccggag
     9901 tagtcgactt ttattttagt ctgattatcg gtttcttatt tttatatttg atcggtttcc
     9961 aactgttacc tttagcgaac cagttccgct atatggttct cgttcagctt tatccaatcc
    10021 ataagaatca gaagaagcag gcgattcagt atctactcac atgggtatta attatcgcgt
    10081 cggttatttt tggtttgatt gctgcagtcg tattagatgg gcagcagcga tgggttccgc
    10141 tagcggctta tttgattgtt actatgctct ttgttaaact atatgtacca actcgtttga
    10201 agaagatgtc agactgaaaa caaagctgtg tagggtaaaa atgagaatga aaaatagagc
    10261 gttgacagta agaatgctgt tcgttctatt ttttttgaga aaagctggtt ttttgaatgg
    10321 caattaaatg aaaaagaacg tatatgtgct tgacgtgtag tgttcaggcg actagaataa
    10381 taagtactgc aaaacaaaga gtaagaggac gggagaatgc atgatggagt ttcagttaga
    10441 ccaagagtgg cggttacgtc ccatcaaagg cgacacagga aaaacataca taggcatgcg
    10501 tgatgaggac aaagtcttta ttaaacgaaa cacgactccg atgttggctg ctctttcaag
    10561 ggaaggaatt acaccaaaac ttgtttggac aaaacgaacg gggaatggcg atactttgac
    10621 agcacaagag tggctggaag gtcgcttgct gactcctaat gagattggtc aaagaaacga
    10681 cgtgattgat gttttgtacc atttacatca ttcaaattct cttaaaacca tgctgacccg
    10741 tatcggtggt cgtgtcatga cgccggagaa gatgctgcaa aaatatgaaa aagaattacc
    10801 ggaagaactc cagaaaaatc aatttctgca aattgtccat cgttttcttc gtgagcagat
    10861 cccgtcaata gataaggaaa acttatcagt agtacacgga gatgtaaacc atcgtaattg
    10921 gttagtatgt aaaaattatt tatatctagt cgattgggat tcaatcatgt ttgcagaccc
    10981 tgcggtggat attgcaacga ttttaggaaa ttatgtgcca ctgtctagct ggaatcagtg
    11041 gctgatcagt tatggcatac gcccgactga cgaaatgctt gaaaaatttc attggtatgc
    11101 ggccatgaat ttgctccaag aaatcaccag atattgcaaa cgtgaagatc atcgacggat
    11161 gaacgaagaa attttacaat taaaacgaat tttcagtggc tgaggtttgg cccaaactct
    11221 gcggagattt ggccagacct ttttaattag aggaaaggaa tggagataaa taatgcgtgt
    11281 ccgaaatcgt ccgggagcag cggaaatgtt agcggctcac ccgaattttg taattagtga
    11341 tccaacattg tggaagggaa aatggaatga attgtttgaa aacgatcatc caatccacat
    11401 tgagattggt atggggaaag gccagttcat cactggaatg gcaaaagccc atcctgaaat
    11461 caattatatt ggagtagaaa tgcaggtcag tgttgtttcg attgcactag acaagttgat
    11521 cgaacaaccg cttcctaacc tgaaacttct acatgtggat ggatcagcgt tgacagagta
    11581 tttcgctgac agcgaagtag atcaaatcta tctgaatttt tccgatccat ggccaaaaaa
    11641 acgtcatgaa aaacggcgat tgacatataa gacttttctt gctgtagatg aacagattct
    11701 tcgtccaaat ggagaaatcc atttcaaaac agataatcaa ggattgtttg agtattctct
    11761 ggcaagtttt tctcaatatg gcatgatcct caaacaggta tggttagatt tacatcaaag
    11821 tcaatttgaa ggcaatatta tgacggagta tgaagaaaaa ttttcttcaa aaggtcaacg
    11881 tatttatcga gtagaagctc aatttcaaga taagaccaaa aagaaaagtc tgtgaaacta
    11941 actttctgct ttcataaaaa cattaatatc cataaataaa tcccagaaaa tgaacattac
    12001 agatgtaatg tattttctgg gatttatttg atttaaaatg tatttatttc atcggttttt
    12061 ccacaaggtg gaatcaattg gaagcgagaa aatgaaaata atcctttgtc ggtgatcttc
    12121 aattcgggta tgacagataa tgttaagaaa gaaagtgtca aaaaaggatc aaaggtttta
    12181 gctgctccta gttgatgtgc tttagttatt aattcattca actgttcata gacgatcgaa
    12241 aaaggttcct gtgtcatgat tccgcctata ggaagagaaa cacaagctat ttcctgctgt
    12301 ttgttgacaa cggtcatccc gccacctttt tgaatcaaaa gattagcagc atacagcata
    12361 tcctcgtcgt ttgtcccaac gacaactaga ttatgcgagt catgggcaat cgttgtcgct
    12421 atcgctcctt gtttcagctg aaatcctttc actatgccta atccaatgtt tcccgtttga
    12481 tggtgacgct cgatcaccgc aatctttaat tggtcttttt cgattgaagg aatgaatcgt
    12541 tcgccttgtc ggtccacttt ttctaccaaa tctttagtca ctagactatt cgggataatt
    12601 tcgatcacat gggcttgtga agaagaaaga agcagctcta acgaatgtgg atttaatggt
    12661 tgagcgttta gttttggcag acaacacgta tatgaatttg aatgatcagc aaataaatgg
    12721 tcttggagtt gcccagattg aacaactaat gttcccttat agaaaacttc atgaattggc
    12781 agcttggtaa gatcgtctgt taagaagaaa tcagcttgat acccaggggc aatcgcacct
    12841 agtgttttca aatcaaaaca ttctgctgca ttcaatgttg ccatctgaat ggcttgtaat
    12901 ggatcgattc caagtgcaat agcttgacgg acgatatgat cgattgatcc ttcttttact
    12961 aaatcattga tcagtttatc atcggtgaca aagaagcatc ttcttgaatt ttcaggtgtg
    13021 atcgccggta gtagcgatgc cagatctttt gcaacggtcc cttctcgaac cattaaatac
    13081 ataccaagat ccaaacgttc tttagcttct tttgcagttg tagcttcatg atccgtccga
    13141 atacctgctg caggataaac atttaactct tccatgccga taccagctgc atgcccgtca
    13201 atttttttct tatgctggtg catcaattgg atcttttcta ggatgtctgt ttcattattt
    13261 gctactgctt gatagttcat gacttcagct aagcctaaaa cttcgggttt ttgtaaaaaa
    13321 ggggctaatt tttttgcatt taaatttgca ccattggtct caaaaggggt tacaggtaca
    13381 caagaaggta gcataaaaaa gatattcatg agtgtttgtc ttgcatcttc tatcataaac
    13441 tcaagacctg tactcccagc aatgtttcct atttcatggg gatctgtcac tacagtagta
    13501 actccatgaa gcaaggatac ttttgaaaat tcgcttggtg ataaaagtga gctttcgata
    13561 tgcacatggc catcgatgaa tcctggcact agatattgcc cattagcatt atgtacgctt
    13621 tttccttcgt attcaccgat tcctacgatc attccttcgc agattgctac atctttttcc
    13681 atgatttctt tcgtaaagac gttgacgatc ttagcatgct tgatcactag gtcagcaggc
    13741 ttttttttac ttgctacttc gatatatttc cttaatgttg tttccaatgg agtgcctccc
    13801 aagataaatt tttataagta taacataaat tcggtttttg ccaatatttt tttgtaaatt
    13861 cattttattt gaaacaaaaa gatcaaaaag acgtatgttt tttgtggagg ggagaataat
    13921 ggaaataaaa cgattatcgg atcaaatgat aagtgaaagt tttgccaaac atgcccaaga
    13981 cgtatatata ttgccagctt cagaagtatt ttacatctat tttcgctatg gaaaaaaacg
    14041 aacattctgg aagaaagtct cttcggcaga tggacagcaa ctgatctcaa gatttaagta
    14101 tttgggacag atggatatcg gagaaaaaag aaaagctcag ttaggtgcat ttacttattc
    14161 tttagaaaaa caggaagttc gtctgcggtt gtcttctgta ggagattacc ttcaacgaga
    14221 gagtttagtg attcggctat tatacggtac ttttgaaaca ccccatcgtt gctttagttc
    14281 tcgagatttt gcaactattt tacagaaaac cgtcagacgt ggattatacc ttttcagcgg
    14341 accagtcgga tcaggcaaga cttctctaat gtatcggttg gctttggaag aaaaattgca
    14401 agtgattact atcgaagatc ctgtagagat agaagaaccg ttttttctgc agctacagat
    14461 caatccgaaa atagcacaga cttatgatac acttttgaga ctcgcattac ggcatcgtcc
    14521 agacttgcta atcattggcg agataagaga tgaatcaact gcacatgctg cggtaagagc
    14581 agcactaaca gggcaccggg tttttgcaac cgtacatgca cgcaatcttg aaggtacatt
    14641 ggtcagaatc catgaactga caaagaaaaa agaagagcta tctgaatgtt tagctggtgt
    14701 catttatcaa gaacttttgg aagatgccaa tcaccatcct tctgttttat ggagttatca
    14761 atttatggag gaagaatcag taaaaaaatc atgggaggaa agttatgaag aagctcagga
    14821 aaatatttgg cagtcagaac tattctaaag gattaacaag aagccagcaa aaccagtttg
    14881 cacaaaccat gggagaactg cttttatctg gttttagtct gcaagaatcc ctttatttat
    14941 tactcacgat ccgttcgttt ccacctgtta ttttggcaaa tatccaacat cagctaaagg
    15001 aagggcaagc gtttagtgag gtactttcgc aaataggata tggagcagag caactggtac
    15061 agatcgagtt agctgaacgt catgggaatc tgccgacaac tttgttggaa attgccaaac
    15121 agtttcggct tgttgaagga tttcgcaatg atttgaaaaa gctgcttgca tatccgatgc
    15181 tattgctcac ctttttgcta ggtatcttgt ttagtatgcg gttgttcgtt ttgcctcagc
    15241 ttattggttc aggaatggtc atgcaagagc attggggtat tcagctgata caagtctttc
    15301 attggtatgt tctactattc atcctagcgg ttactctatt ggctttacta gtaaggtatc
    15361 gtctgatgaa gttgtctcct gttgatcaga gtgaatggat aagcagacaa ttattgattg
    15421 gaccgatata ttccttgtat caatcggctt atttgtcact agagattgga aagctttttc
    15481 atgaaggttt cgaattaagg caaatcatag tctgcttaaa ggaaacgaaa caagaaagtc
    15541 tgattcagca tcttgcctct aagttgattc aaggattgga ggcaggtatg tcattggctg
    15601 agcagttcca gaattatcgt tttctatcag aagaattcag taaaattgtt ttacagggag
    15661 aggcaaaggg aagtttaggt aaagaactga ttttttacag cgaatggaca agaaaacagc
    15721 ttttcaaacg tcttcatcac tggatgcatt ttattcagcc aatcattttt ttgggcgtag
    15781 cggctttgat tttattagtt tatgccgctg ttttattacc tgtttacgga agcatagagg
    15841 aggttttgcc atgaaaaaga gaaaaatatc ttattatagc ggttttacat tgatcgaaat
    15901 gctaatcgtc ttactgatca tcagtgtatt agtgttgttg ttcgtcccta atttgtcgcg
    15961 ttataggaat catgtagacc aagaaagtcg tgaagcgatc atccagttgg tagacacaca
    16021 aaaagagctc tatgcgcttc aaaataatgg aagagttcct actgtggagg aattgttgaa
    16081 tgaaggctat atcaaacgag aacatgcaga aatctatcag cggccttgaa ggtaaggttt
    16141 tagcatcagg atatacgttg tttgaatcac tcttactgat tttattactt acaacgatcc
    16201 tttcttttcc agttctcgca ttttctgcat ggaagaatga attggcagtc tatcaatttt
    16261 tcggccaatt tgaaaaacga atctattcta cgcaaaaaat cgctattgtg aatcagatcc
    16321 aaacaggctt ttattggaat agtgaagaga atcaaatcat ttttcatgtt ccgaatccag
    16381 ataaaataaa ttgggaagta ttagatattc ctgatgagat taccttgaaa cgccatgctt
    16441 ctattacttt tgctgctaga accgggaatg aaagttcact aaaagcttat caattttatt
    16501 gggaagaaaa agagcggacg attacgtatc aattccagat agggagtggt cgctatacaa
    16561 aaagaattga ataaaacagg atttgttttg ttggagtccc ttacagcgct tatgatcgct
    16621 tctgtctgtg tctacttagt gagctttggc cagctgtttt tgcttcaggc agaaaagaaa
    16681 agtggggagg aactgctatt tattcgaatg atttatgaag atatcaagac gcagcggatg
    16741 tatcacaaaa gaccagagcg aagggaagaa atgatcccat acgatatcca aattgtcgat
    16801 aataatggag taatcgaaaa agtgtttgtc aatatggaga aagaagcgat cagtattgaa
    16861 aaaacaagta attccagcat ttacgctgat tgaatgtcta attggactga tgatattgag
    16921 cagtctattg ctcacattca atcttttgat tcagcaaacc atccaagtaa gtgaattttt
    16981 gcagcgtaaa gatcaaaaag agtggctaat atttttagca caattagaag aagaactcaa
    17041 gagcagtcac aatgtctctg tgaaaaatca gcaactgcac tacacgatcg aggataaaca
    17101 gtatatcatc gaaagatatc aggcaatgat acgtaaaaga agaacttccg gcggacatca
    17161 acctatgtta acaagtatca ctgagcttca attattggaa aaagaacaca caatttcttt
    17221 ttatgtacac tttgaaaatg gagaagatgg atatgccata tggacaaaaa acgatcagta
    17281 gaaaatgtta taagggctct attttatttt ctgtcttgct tttatttctt ttatgtacgt
    17341 ttttattgat ggcagcatta gaaagctacc gtttgtcaat ggatcttaat atacgaatca
    17401 aaaattatta tatagcaaaa ataatgaagc agatggcttt atcggaacag tctacgcttg
    17461 ccgaaagttc ggaaggagtt ttttactaca caactggatc tgtaacatat cagtgggtac
    17521 aacagtccct attcttagag gtggaagtct caccgtttat ctttcggttc acagaagaag
    17581 taaaaaatga tacagatact agtacagaat gatggaatgt tcgtgaaaac gccctctttt
    17641 ctgttataac actgttttta ataaaaaaat gtttgccgtt tcacaaataa aaaacagtga
    17701 aaacgattga aattttgtca attgttcgat agaattaaac aggtgaaaaa attgttataa
    17761 tatatctaga aatgaggtgc accatgttac cagaaaaaat ggaacaaggg tttaatcaaa
    17821 acctggaagc tattcagctt ttacagaatg cattgggaac ttccttttta gaagcctatg
    17881 tcgaaaatgc agaaaattta atagatgatt atcaagtacg tgtggtggat ggagtgccga
    17941 caaaagagac aacgcaacgg atcacagctc tttatgaaga gttgaagaaa ctttctttcg
    18001 agccagaaga atggcgcaga ctgtctcaat tacttttatt gaagggcagc cagacagaac
    18061 atttgcaggc gaaccatcaa ctcactcctg atagtatcgg ttttttattt gtttttctta
    18121 ttgaacaact atacacaaat aaaaaagcac cggttaagat tctggatatt gcggcaggaa
    18181 tgggcaactt gttattaaca gttcttttaa acctatcgaa tgcggggtac caaacagaag
    18241 gaattggtgt ggatattgat gatacattgt tggctgtcgc agcttctaca agtgatttga
    18301 cacaagcaaa tgttcaatac tttcatcaag acgggttgca ggaattatta atagatcctg
    18361 tcgattttgc aatcagcgat ttgccaatcg gttattatcc gaatgacgaa aaagcaaaag
    18421 agtttttaac aagtaccgaa gaaggccata gttatgcaca tcatttattg ctagagcaat
    18481 caatgaaata tgtgaagccg gatggttttg gtttgttttt gatgccgagt ggatttttag
    18541 aaacggatca aagtgaagaa atcaaaaaat ggtttaaaga agaaggttat cttcaaggaa
    18601 tgattcaatt gccagatgaa ttgttccgta ataaacaatc gcaaaaaagt attttgatct
    18661 tacagaaaaa aggaccacaa gcacaacaag taaaagaagt cttgcttgtg aaacttgctt
    18721 cattaaaaga accagagaag gtgacggaat ttttcaacga gtttaaaaac tggaaatcat
    18781 ctgctctgta atcattcaac gagaaggaga gtcatatgtc taaaacaatt gcaatcaatg
    18841 ctgggagttc aagtttgaaa tggcaattat atcaaatgcc agaagaaacc gtagttgcga
    18901 aagggatcgt cgaacgtatc ggtttacaag attctatttt taccatcaag tacggcgata
    18961 atcaaaaatt cgaacaaatc atggatatcg aaaaccacga agtagcagta aaaatgctgt
    19021 tagatcagtt gattgaactg caaattttaa gctcttatga tgaaatcacc ggagtagggc
    19081 accgtgttgt tcacggagga gaaacttatg gggattcagt agtgatcgat gagaaagtaa
    19141 tggaccgcat tgctgaatta gcagaatttg ctcctttgca taatcctgca aacttaatgg
    19201 ggatcaaagc gtttaaaaag attttacctg acattttgag tgtggctgta ttcgatacat
    19261 ctttccactc aacaatgcca aagcaaaatt atttatatag cttaccggtg tcttattatg
    19321 aagatttcaa agtaagaaaa tatggcttcc atggcacaag tcatcgttat gtgtcggaac
    19381 gtgcggcaaa attattgaac aaaccgatcg aagaactaaa aatcatcact tgccacttag
    19441 gaaatggtgt atcgatcaca gcagtcgatg gaggtaaatc catggatact tctatgggat
    19501 tcacaccatt agctggggtg acaatgggta cacgttcagg tgatatcgat ccagctgttc
    19561 tcccatattt aatggaaaaa ttagagattg atattgatga aatgatcaat gttttgaaca
    19621 agaaatctgg tctgctaggc ctaacaggat tgtctagtga tatgcgtgat cttgaaaaga
    19681 actatgaaaa agaacatatt cgtttagctt atgacatttt cgtggaccgc atccgaaaat
    19741 acatcggcgg ttatgtgaca acgatgaatg gtgttgatgc aattgtcttt actgctggta
    19801 tcggcgagaa tgacggacat gtacgtagtg aaatcatcaa aggtatgtct tggtttggtt
    19861 gtgaaatcga tccagcgttg aatgaattgc gtggtgaaga agtagatatc tctacaaaag
    19921 aatcaaaagt aagagtatta gtgatcccaa cagatgagga actaatgatt gcccgtgatg
    19981 tggagcgtct gcgcggctaa gccaaagcat tcgttaggat aaaagatgaa acgcatgcaa
    20041 gagggaattc ttgcatgtgt tttttttgcg gagaagaccg caaaatggta atttaacttc
    20101 aatttccctt cttttttaca ggatcttctt agggaatttt gcttggaaaa gtggtcaaaa
    20161 gggaaaagac tggcatttta tttattgatt ttcaggaaaa tcccaatatt ttattttcat
    20221 tcatctttat aagggacatt acatatgtaa aaagaaaaaa catttttaga tcagcctcgt
    20281 gggctgtcga ctttattctt cgctttatgt ttagctagaa aaccgatccg aaacattcat
    20341 tgatcgaata ataaaaactt gattttccac ctgtactttg cctgaatcgt gaaagcacag
    20401 gtatgaaaat caagtttttt gttgcgaaaa aacgtcctag tcaatcagtt ggccgagcgg
    20461 ctggcggctg atctgctcct gatcaataac gagtgcttct tggaagtgtt gctgcaaaaa
    20521 gtagcggtca tggctgactg ctaggagcgt tccttgatag tcttgcagca tttcttcaat
    20581 ttcttctcga gcatagctgt cgaggtgatt ggttggttca tccaaaatca ggacgttgat
    20641 tttttctaaa aagagtttca gcaacgccaa acggattttt tcaccaccgg ataaatcttt
    20701 gatccgttta cccacatcgc tttgataaaa gccacttttg gctaattgcc gtcgagcgac
    20761 ttcttcgttt ccagtcaatg tgatggcgta agccagcaaa cggctatcgg gatcggcaaa
    20821 ctgcagctgc tgcggcaaat aaccgatttg caagctacta cctaatttga tggtgccacc
    20881 gtaaggcttt tcctcaccga gtaataattt gagtaaggtt gattttcctg caccattgtc
    20941 gccgatgatg gctaaatgat ctccacgata gactgcaaaa ctgctgtctt tgaatagtac
    21001 ttgatcagcg tactgctggt gcaagttttg aatgatgaaa acttctttgc cagattttcc
    21061 accattagac aagctttgca gccgattttt cgggggaggt ggcggtttga cgagtgtcag
    21121 tttcgccagg cggcgttcca gttctttggc ttttttgaag aaggactcat tgtctccttc
    21181 atgtgcccac tgacgaaaac gacgaatcat gactttcaac cgctggactt ctttttgctg
    21241 cagttcgtaa ttttttgtca gctcagccaa gcgagcttct ttcaagactc gaaaacggct
    21301 gtagtttccg ggatactcaa tcaatgcacc ttcttccagt tcccatgttt ttgtggtgac
    21361 ttgatcgaga aattgccgat catgagaaac gaccagaacc gatttttttg tattctgtaa
    21421 atagttttcc aaccagcgaa tactgttaag atctaaatga ttggtcggtt catcgagtaa
    21481 aagtaaatcg gcatcggctg ctaaaatttt ggctaaagcg actttaactc gttcgcctcc
    21541 gctaagttct gttaaccgtg cttcctgacg gtgtggaatc cctaagcctt gcaagatcgc
    21601 tgcaatccgg tcttctaata cgtaaccgcc tagttcttca aagcgatgct gcagctcacc
    21661 gtaacgggcc agacacttct ctaagtttgt atctggttct gtcatcaatg cttcgtagcg
    21721 actcatttgc tgtttgaggt cctgtatctc agcggcagaa tggagcaagt agtggttgac
    21781 ggtcgcttcg gaaactgtaa gttcttgttc gaccgtcccg atctttaagc cttttttgcg
    21841 actgataaca ccgctgtcga caccttctct gccggtcaag atttgtaaaa aggttgattt
    21901 gccagttccg ttgataccga ctaaggcaat tttttcacca gcttcaattt gacaggataa
    21961 atcctcaaat aatggattcc cgccgattgt ttttttaata gaaattgctt gtatgatcat
    22021 gatctctctc cttgagacca ctcaaaaagg ccatggttat ccatggcctg tgttatttaa
    22081 actcagatta tcatgagaaa aataccttga gatggccatt gattttacgt gaattcaact
    22141 gttttggaca aacttgtccc gtcaacagca actgtaaaac caattgcatg gctaaatgcg
    22201 atatggtatc gttggtaatt ttcattactt gttattttag tcatgcgcat catagtattt
    22261 tccctccttc gtgtagttgt cttttccagt gtacctgaaa gtgtcagaaa gttcaactag
    22321 cgctgacatt tacttaatat ttatcgatgt gtaattctgt cagagagctt tttttatctg
    22381 actgagggta atgtctcgtg gaccacatca ttgtattctg agctaaacca gcgttcaggc
    22441 gaaatcatat ggtgtttgat aaaaaacttt gtctacatga cttccttttg ccatttgctt
    22501 aataccgttg cattaaacag ctcttctctt tggaaatctc accaacatcg cgttcaggag
    22561 tatcgttgtg gttgcaatgg caagcggtag cctttcatca attcttgaat gatttaccga
    22621 atgtagcact tgccttctcg cggtacccga acccgaaata aaattttagt ttggcgataa
    22681 aatcggtatc cgccatcgat acttcatatt ttttcgtatc agattcatcg gaaacttcca
    22741 cattgacgaa ccaataagct ttggcatgct taggtcgttt taaaccacac cagggcgact
    22801 aatcgccctg gtgttcactt ttttttattt tacttgaact cattgactgt tttcccttta
    22861 ttttaaacat tataaagacc gatgagtgct tgccgcgtct tttgttttta tcacggtttt
    22921 ttgtgcaaaa aaacctgccg atagttggca ggtcgtctac gtgttaagct ttggtgtggt
    22981 ctttagctgg caattggttt tctaaatcag ttcggacaac aagcacatcg caagctgcgt
    23041 ttctaataac atattcagaa acagaaccaa taaataaacg ttctacagca ttcaatcctg
    23101 tggctcctaa cataatcaaa tctacttgat tatcttctgg gatttctctg gcaataattt
    23161 gttttggtga gccatattct acgacagagg ttacattatt caagccagct ttcttcgcat
    23221 tgctttcata atctgccaat gtttgctttg ccatttcagt agcttgttct gcaagcattc
    23281 catcaaagga tgaaacggtt tggaaagaac gtgtgtcaat gacatgtgct aatagaagtt
    23341 ctccgttatt tcggatagct acattgaccg cctttttaaa agctaattca gcttcatcag
    23401 aaccatctac ggcaaccatg atttttttgt attgttgtaa cataagcatt cactcctcaa
    23461 gtcaatcgta ttgtactctt ttattgtaga acactttagg gaaaaagaga attatttact
    23521 gctttggttc agcaattttt tttgcaaatc ggatactgaa ggtggcagaa acaataatta
    23581 cgatcagtaa aaagaccaag gcaataaagc gttgattgaa aaaagctgtt aaaataccca
    23641 ttacgcctaa aacagcatat gcagctccgt aaaattgtag gaatccttga tttttatcat
    23701 ttttgttgat caatacaaaa aagatatcct gtttattaaa taaataatag ctaacgaata
    23761 ataatactaa agtggcgata atcattaaaa tcctaatcat gaacaaatcc tccaaaataa
    23821 gcaacaaata gaaaaagcaa tttgccaaga gcaaattgct ttattcttta aaagatggta
    23881 atctccgtta atgccgttgt ttgtcctgta gtattgatcc cgcgaattca agcaggtggg
    23941 tcccatggtc gtagcttcga actaccaaat gataaggcat gttaccagca gtgacacaag
    24001 acgggtccca agatatcaat aaaaatgttc ggtcaacaca caaagaggac aattcgaaca
    24061 tcacagattt cccacctcta tagtagcata tgaatttaaa aagagcaatg agagagctcg
    24121 cagaaacaga accgttttct tgtctagttt tttggaggat gctttttcca ctcttttata
    24181 cggtaatact gttgacccag agcctgttca tatttccctg tatcttttgg ctgataatac
    24241 tgagcattct tcagtttatc tggtaaatat tgctgattga cccaagcctg atcaaaatga
    24301 tgaggatact gataacccac gccacggttg agtgatttcg cacctttgta atggctatct
    24361 ctcaaatgat caggaacatc tccagctttg ccctctcgaa tatcagcaag tgctgcatct
    24421 aatgccatgt aggcagaatt ggattttggc gacaagcaaa gatcgacgac gacgtctgct
    24481 agtgggatac gtgcctcagg caatcccaat ttttcagcag ctaatacagc gttcactgta
    24541 cgagccgcag cagcaggatt acctaaacct atatcttcat aaccgataac catcagacgc
    24601 cggcaaatac ttgccagatc acctgcctca actaaacggg cgagataatg aagtgcagca
    24661 tccacatcac ttccacgaat cgatttttga aaagccgaaa taacgtcata atgagcatct
    24721 ccatttttgt catgggtaag agcttttctt tggacacatt cttcaatgat cgacagtgta
    24781 agatgaatcc ttccttcttt atctgaagga gtcgatagtg tagccagttc cagcccattc
    24841 aatgcactcc gcaaatcgcc gttcgttgca cgcgaaaggt gaagaagagc atcttcgtct
    24901 aactggattg cttgttgtcc aaggccgcgt tctttatccc ttaaagcatg ttcgactgcc
    24961 aactggatgt cttgttctgt taatggtttg acttcaaaga tctgtgtacg gctgcggatc
    25021 gctggattga tggtaatata aggattctca gtagtcgctc cgatcaagat gattcggcca
    25081 ctttctaaat gaggaagcag aaaatcctgt tttgttttat ctaatcggtg tacttcatcc
    25141 aataagagga tcactgtacc actcattttt gcttcttccg ctacaacttg tagatccttt
    25201 tttgaatctg tagcggcgtt tagcatgcga aatgcataat tagtagagcc ggcgatcgca
    25261 cttgcgatac tcgtttttcc agtaccagga ggtccatata gaatcatgga agagagcatg
    25321 cgagcatcga ccatgcggcg tatgattttt ccttccccga ctagatgctg ctgaccaacg
    25381 acttcatcga tcgttcgagg tctcatgcga taagccaatg gctgttgcat aggattttcc
    25441 tccttcttgt ttttacgtcc cttaattata tcatatttag caagatgcct agaaagaaca
    25501 gccttctgtt gttttgtttt catagcttat ccaactaata ttgtgtatga taaagtaggt
    25561 gagaaaaatg acggataaac aaaagataga tgaatttttt tggaataaaa cgacgccaga
    25621 agtggcacgg gatttattgg ggatgtattt ggagcatgac acaccagaag gaagattagc
    25681 tggatatatt gtagacgcgg aagcatacct aggtccagaa gacgaggccg cccatagcta
    25741 tggtttaaga agaacaccgc gtgtgcgggc aatgtatgaa aaaccaggta cgatctacct
    25801 ttacacgatg catactcatc ggattttgaa catcattact cagccagaag ggattcctca
    25861 aggagtaatg atacgtgcaa tcgaacccgc cgcaatgatt gatcaaatga gtaaaaatcg
    25921 tggcggaaaa actggtccag atatcagcaa cggtccggga aaattagtgg aagcattagc
    25981 aattcctcaa gaactttatg gtcagtctat agcagatagt tcgttacgct tagtttttga
    26041 gaaaaaaaag acaccaaaaa agatcaatgc tttgcctaga atcggtatcc caaacaaagg
    26101 tgtatggaca gaaaagccgt tgcgttttgt tgtgtcggga aatccttata tctctttaca
    26161 aagaaaaaac caaatcgaga aagattgggg ttggaggaaa gaaaatgaaa aagaaggatc
    26221 tattaacata tttagatgaa ataattgaaa aacaagtgac agactatgat gtagctttag
    26281 attgggatac aaaaaatcat acgatcgaga tcgttgttcg cttgtttgca gaaaataaag
    26341 cacaatttga gattgatgat gcagaaggga tcgtttcgca agaaccaatt atcgaatttg
    26401 aagacggcgt acttcttttt aatcctcaga aatctgtttt tgatgaacgc aattatctag
    26461 cagtaatccc ttatgaagga aaaaaagggt tggcaaaatc agtagctgat tctttagtgg
    26521 aatatctaaa tgaagtatta gctcaagggc aaagcgattt gcttgatttt ttggatgaag
    26581 acgatgacga agctgtattc gagcttcact gggataatca gcaactagca gaattagtag
    26641 aagtaaaaca gcaaaatgaa gcagatactt atttaccgta cccaagttat taaggagtga
    26701 cagatatgaa atggaatgaa gtaaaagtag aaactgaaag tgaagcagtc gaagcagttg
    26761 ctaatatttt gatggaggca ggggcgagtg gtgtcgcgat tgaagattct ttagatgttg
    26821 aacattttca gtcggatcaa tatggtgaga ttttagataa aaatgatttt acatcgctga
    26881 aagaaggcgc actagtcatg gcttattttc cggaaacgac tttcttaccg gaaatcttgc
    26941 cttttatcaa agatcgtatc acacaattac ctgagtttgg tctagctatt ggaaaaaata
    27001 aagtaacagt tagtgaagta gaggaaagca actgggcaac agcctggaag aaatactatc
    27061 atccggtgcg tattacgcga atgctgacgg ttgcgccaag ctgggaaaac taccagacag
    27121 aagatcctct agaaaaaatt atcacactag atcctggtat ggcatttggc acaggcactc
    27181 atcccacgac ttccttgaca ttgacagcac tagaaacagt cttgcgtggt ggtgaaacat
    27241 tgctagatgt gggaactggg tcgggcgtgc taagtattgc agcaaaatat cttggtgcaa
    27301 aagatgtcta tgcctttgat ttagacgaag tagccgttcg ttcggcgaaa gaaaatatgg
    27361 atatgaatga aatagccaaa gatgtccatg tatcagctaa cgatcttttg acaggaatcg
    27421 agattgaaag tgatgtaatc gttgcgaaca ttttagcaga tatcatctta ttgatgatcc
    27481 cagatgcttg gcgtttactg aagcaaacag gcacgctgat cgtttcagga atcattgaag
    27541 agaagaagca aatggttctg gatgccatgt tagaacaagg gtttatcgtg gatcaaattt
    27601 tccagcaaaa agactggtat gcaattattt tgaaaaaacc agaggtggaa tagatgcagc
    27661 ggtatttttt aaaagaatca tatgaatcaa aagactattt tgttttagaa ggggaaaact
    27721 atcaccacat tgtacgggtt atgaggatgg aacccgagga tgaagttttt ttggcattca
    27781 atgatcaaat agccattgtt gcagaaatca cggagattac tgaagaggct gtttatctga
    27841 aagaaattaa aaaagaagaa aatcaaaagg aactgccaag ttcgattacc atagcgagtg
    27901 gctatcctaa aggggaaaag ctggattgga tcgttcagaa atccactgaa ttaggagcgc
    27961 aagcttttct tggtttcccg gcatcagcct ctgtcgttaa atgggatgca aaaaaacgta
    28021 tgaaacgaca acaacggctt gaaaagattg ctcaagaggc agcggaacaa tcccatcggc
    28081 agatcgtgcc atcagtagag ttattggaaa cgaaacaaga tttatttgag cggctagatg
    28141 attatgacgt ggtcttggtt gcttatgagg aatcagcaaa gcaaggcgaa aaatcaaatg
    28201 cagccaaaat tcttagtgaa acaaaaccag atagtcgaat cttagctgtc tttggaccag
    28261 aaggtggatt atcccgcgat gaagtcgatt attttgtaga acacaaaggg atactttgtg
    28321 gattaggacc aagaatcctt cgtacagaaa cagcacctct ttatttatta agtgctgcta
    28381 gctatcaatg ggaattaatg aatccttctt tcagctaacg ttcttcatta ataaaagagt
    28441 atcgacagaa agaattgaaa aaatagtcaa caatttgtat aatagaaaca attatttata
    28501 caatggatac gaaaaatcgt ttgaaaaagc ttttaaaaag cgaacatttc cgtaaaaaaa
    28561 cgtataactg cacggatttt gggacaatta tcaaacagat atttgtcccg attcccgttt
    28621 taccgaaaaa agaaaggggg atcgattcct gtgacaaaag aagaaatttt aacgggaccc
    28681 ggtgtcataa agctcgttag tcaatacatg ggtcccgaac atgtagcatt tgttgagaaa
    28741 gcatgcgaat acgcgactgc tgcacatgac ggacagttta gaaaatcagg cgaaccctac
    28801 attattcatc ctatccaagt cgcaggtata ttagcagatt taaaaatgga tccccataca
    28861 gtggctacag gcttcttaca tgatgttgtt gaagatacag aaatcacttt agaagatctg
    28921 agagaagaat ttggcgatga cgttgctatg ttagtggacg gcgtaaccaa attagggaaa
    28981 ataaaatata aatcccacga agagcagcta gcagaaaacc accgaaagat gctgctagca
    29041 atggctcaag atttacgagt tatcatggtc aaattagctg atagactaca taacatgcgt
    29101 acgttgaagc acctgcgaga agataagcaa agaaggatcg ctcaggaaac tttagaaatc
    29161 tatgcgcctc ttgcacatcg tctagggatc agccggatca aatgggaatt agaagataca
    29221 gcacttcgtt atctaaatcc aaaacagtat taccgtatcg tccacttgat gcagacgaag
    29281 agagaagaac gtgaaaaata cgtaagtggt actgttgaag atattcgaat agcgacagaa
    29341 gagctgggga tttttgcaga aatctatgga cggccaaaac acatttattc aatctatcgt
    29401 aaaatgaaag atcagaaaaa gcagttcaac gaaatttatg acttgctagc tattcgagtg
    29461 atcgtagatt caatcaagga ttgttatgct gtattaggag caatccacac aaaatggaaa
    29521 ccaatgcccg gcagatttaa ggattatatt gccatgccaa aagctaatat gtaccaatct
    29581 ttgcatacta ccgtgatcgg tccggcagga aatccggtag aaattcaaat ccgaacacag
    29641 gaaatgcatg aaatcgctga attcggggtt gctgcacact gggcctataa agaaggaaaa
    29701 aatgaaaaag tagaaccaga tggtatgacg aaacaattaa gctggttcca tgagatactc
    29761 gaacttcaag acgaaagcta tgatgcttct gaatttatgg aaggcgtaaa aggagatatc
    29821 tttagtgata aagtctacgt cttcacacca aaaggagacg ttactgagtt accaaaagga
    29881 tccggaccat tggactttgc atacagtatc catacagata tcggtaacaa aaccactggt
    29941 gcaaaagtaa atggcaaaat ggtgcagctt gattacaaat tgaaaaacgg agatatcatt
    30001 gagatcatga cttctccaaa ttcatttggc ccaagtcgcg actggttgaa attagttgct
    30061 actagcaaag caagaaataa gatcaaacgt ttcttcaaag cccaagatcg agaagaaaat
    30121 gtgatcaaag gccacgaatc cgtggtcaaa tgtattacag atctaggatt tacgcctaaa
    30181 gatattttga cgaagaacaa actgcaagaa gcactcgatc gttttaatta tcaaacagaa
    30241 gatgatctct atgcagctgt agggtatgga gaagttagcc ccttgacgat ggccaatcgt
    30301 ctgactgaaa aagaacgtaa agaacaaaaa atcgagcagc aaaagcaaga agcagaagaa
    30361 atcatgaatc agccgaaaaa agaacctgac aaaatgaaag tacgtcatga aggtggcgtt
    30421 gtcattcaag gggtagaaaa cttactaatt cgtatcagtc gctgctgtaa tccgattcct
    30481 ggtgatgata tcgttggtta tatcactaaa ggcagaggga tatccattca tcgtcgagat
    30541 tgtccgaatg ttcagcctga caaaccaaat gtagcagaac gtttgattga agtcgaatgg
    30601 gaagatacat cgaatacacg aaaagagtat gatgcagatt tggaaattta cggctataat
    30661 cgttcaggct tattgaatga tgtacttcaa acagtcaatg cgctaacgaa aaatctcaac
    30721 agcgttgaag cacggacgaa taaagataaa atggcgacga tccatttgac ggttggtatc
    30781 cagaatttat cccacctaaa gagtatcgtg gataaaatca aagcagtacc tgatgtctac
    30841 agtgtacgcc ggacgaatgg ataggaggac agatatgaga gcagttgttc aacgggtaag
    30901 taaagctgct gtgtcagtcg atcagcaaga aattggaaaa attggctacg gcctacttgt
    30961 tttattaggc attcatgaaa ccgatacgca aaaagatgtg gactatctgg taaaaaaaat
    31021 tgcacaaatg cgtatttttg aagatgaaca aggaaaaatg aatattagtg tagaagatgt
    31081 gggaggacaa attttaagta tctctcaatt cacactgttt gcggatacga aaaaaggaaa
    31141 tcgaccaagc tttgtggaag ccgcacgacc agaaacagct attcctttat acgaatcatt
    31201 caatgaagga atcagaagtc gcggcatcac agtcgctacc ggacaattcg gagcagatat
    31261 gtcggtttct ctcgtcaatg atggacctgt aacgatcatt attgattcta aaaacaaata
    31321 aagtaatctg gttttcacta gtaaaaacta tgaaacacgt cgcttaagtt agactgataa
    31381 actctaactt aagtggcttt tttgtctgca aaaagcgctt cttttgtcag cagtcggaaa
    31441 aaaagttaag cttaaattat acaagttatt ttttatagga ggttgctgaa gatgaaagaa
    31501 gtaccagtag tagatatcaa aatcacagat ttaaaaaaat tgtatcaaaa aggatacaac
    31561 atgttggagg agttacgcca tgaagcggac gctcccgtcg tcaaagcaaa gattttcaat
    31621 aaagaagcta tcacgatcta cggatcatca gcagcaaaag tattttatga tccaaggaat
    31681 ttcaaacgaa aaggtgctat gccaaaatta gtattgaaga cactttttgg tcaaggtgga
    31741 gtacagactt tggatggagc tgctcatcac catcggaaaa atatttttat ggatctaatg
    31801 acaccggaaa gaatggaaga ttatcatcgt atcttagata agaatctgac acaagcactt
    31861 gaagcacagc atggtcaatt tgaactattt gatttaagta agatggtttt ctttacatct
    31921 atttgcgagt gggcaggtat caatttatca gcgatttcta aagatgaggt cgaaaaatta
    31981 gcagaatatc aaatctctat gatcagcgga acgtttactt ctccgattga tcacataaaa
    32041 ggtgtcgaaa atcgcaaaaa atcagaaaaa tgggcgcaag gcttgattga agaagctcga
    32101 caaaatcctg tggctggaaa agaaaatgtg gcattatatg cttttgccaa tgccacagat
    32161 ttagatggtc agcttttacc tctggaagtg gcagctgttg aattattgaa catcatccgt
    32221 ccaacggttg ctctaactgt gtgggcggca ttgatgggac atgcactttt cagtagacca
    32281 gatttgtatc agcaattaaa aaatgacttt tcgacgttac aagatccgtt tattcaagaa
    32341 atgagacgct attatccttt cttcccaatg ctacctgcga tttcgctgaa agaagtagaa
    32401 gtagatggtt atcgtatacc agaaggcagt tgggttattt tagatttata tggaacagac
    32461 catgatgaac gaacagttga agcgccagat tcctttatga tcaaacggta tgtagggaaa
    32521 gcaaaagata tttcctataa agaagaatat gagatgattg cacaaggcgg gggaaatttt
    32581 cgacaaatgc atcgctgtgc aggagaatgg atcactcttc atagtctacg tgtgttttcc
    32641 gatcagctag taaataagtt tgagttctct gtacctgaac aagattggac gattccattc
    32701 aatcagtttc cgacttatcc gaatagccga gcgctacttt acaagaatta gattagcata
    32761 aacttatcga aaaaaaggaa gatcaaacag tgctgatctt ccttttttca tattactata
    32821 aattgataag gtcaattgat caaaatccac gaaatgcttc atcaatggtt tgataatctt
    32881 cattggataa atgcaaatct aaagcttgga cattgtcaga tacttgctct ggacgcttag
    32941 ctccaggtat aactacagac actcgtggat tcttcatgta ccaagctaaa acaagttgcg
    33001 caggagtggc ttggtatctt ttagcaatgg ggcgaagtac atcgacagcg gttaaaattt
    33061 ctctgaaacg ttctccttgg aaatctggat ttctttttcg aggatctcct tcaccaaatt
    33121 gtttttcctc accaagttca tattttcctg tcagcaaacc ggaagcaaga ggaaaatagg
    33181 gaacaaaact gattttgttt ttttcaagat aagggaataa ttctttttca gcctgccgat
    33241 ggatcaaact gtatttatct tctactacat cgacatatcc atctgcattc gcttctttca
    33301 attgttctaa cgtgaagttt gacacaccga ctgctcggat ttttcctgct tcttttaatt
    33361 cgtgcaatgt agcaactgat tcattttttg gtgtgctttc atccggaaag tggatataaa
    33421 aaatgtcgat gtaatcagtt tgcagacgtt ttagtgcatc ttccaccgat tgtttgagaa
    33481 actcaggaga attatcaaat gttcgccctt tatttggtac atgagcagct tttgtagcga
    33541 tgacgacacg tgagcgatca tattcttttt ccttcagtac ctcgccaatc agttcttcgg
    33601 aacggccgtt tccgtaagca taagcggtat cgatcaaatt gatcccgcta ttcaaagctg
    33661 tacgaactac ttgttttcct gtctcatctt ccaaaccgtc aaaaagatta tgtcctccaa
    33721 cggcatttgc tcctaatcca agctgttctg cgtagacttg tgaatgtccg attcttattt
    33781 ctgccatagc tgattcctcc tttttgtcta gtctaaaaca aaataccaaa aagctgaact
    33841 aaaacgtttt tgtgagggga aatagtcaga tatctcttga ctttgagact ggtttttagt
    33901 aggttataag taatatcgaa tgtcaatgaa agagcgagta agtttttgaa actttacaag
    33961 agaggatcat cgaggctgaa aatgatctaa agtttcatta gcctaaagac actctggaga
    34021 tggacgagtg aaaagccgtc ccgcgtacga gcgttattcg taagaggaaa gagaaatctt
    34081 tcaaaattag gtggtaccgt gtatgaattt cattcaccct tgttcatgat gaacaagggt
    34141 gattttttta tttacatagc taagaaaggg agagaaaact atgagtttcc aaagaccaaa
    34201 aggaacaaat gatattttgc caggcgaatc agaaaaatgg cagtttgtcg aagaaacagc
    34261 acgtttatta ttcagagatt atcagtataa tgaaatgcgt acaccgattt ttgaacacta
    34321 tgaagtgata tcccgaagtg taggggatac aacggatatc gtttcaaaag agatgtatga
    34381 tttttatgac aaaggggaac gccatgtgac gcttcgacca gaaggtacag caccaatcgt
    34441 tcgttctttt gttgagcata aattgttcgg cccagagtat gcgaaaccgt tcaaaacttt
    34501 ttatatggga ccaatgtttc gctacgaacg ccctcaaaaa ggacggttac gtcaattcca
    34561 tcaaatcgga gtagaagcat ttggatcaga aaaccctgca actgatgtgg agagcatggt
    34621 gatggcactt gatttcttca aacagttggg gatcacgcaa atccggctag tcatcaattc
    34681 attaggtgat aaagaaacgc gtcaagccta tcgtcaagca ttgatcgact atcttttacc
    34741 ttttgaatca gagctgagtg aagactctaa acgccgcctt catgaaaatc cattacgcgt
    34801 attggacagt aaagacaaac gagatcaaaa atttgtggca gaagctccat ctatcttaga
    34861 ttatctaagc gagcctgctc aaaaacattt tgataccgtt gtttccatgc tggatgcatt
    34921 ggctgttcct tatgaaatcg acagtaatat ggttcgcgga ctagattatt acacacatac
    34981 gatttttgaa atcatgagtg acgcaccaaa aatgggggca caagcaacga tttgcgcagg
    35041 cggacgttat gacaatctag tggaagaact aggtggtcct tcaacacctg gcttcggatt
    35101 tgcaatgggt atcgagcgat tactcttgac tatggaggca gaggaagtag ttatccctgc
    35161 atttaatgag ttggatgctt acgtggtcgc actcggagac gaaacaaata tcgaagcatt
    35221 gaaagtggtc caagcgattc gaaactttgg tttttcagct gaccgcgact tcatgaaccg
    35281 taaagcaaaa gcgcaattta agacagcaga taaagcgaat gccaaattag tattggtgat
    35341 cggtgaagat gagcttgcta gtcaaacgat taatgtcaaa gcaatggcaa gccgaaaaga
    35401 aaaaagtttt ccattagaag agatatatga acattttgat gaagtctatg atgaaatgac
    35461 tttattcgag taggaggaaa aaaataaatg gcacaaagaa cgatctattg cggattagtt
    35521 tccgaacaac aattagaaca aacagttaca ttaaaaggat gggtacaaaa aagacgagac
    35581 cttgggggag taatctttat cgacctacgt gatcgtgaag gaatcgttca aatcgtcttt
    35641 aatcctgaaa tcaacaaaga agcatgggaa atcgcggata aatgtcgcag tgaatacgtg
    35701 atcgaagtga ctggggaagt tagaagacgc ggagaaaatg cagtaaatcc taaaatggcc
    35761 actggtgaat tcgaagtgat tgcaagtgaa atcgtgattt tgaatcaagc aaaaaccccg
    35821 ccatttcaaa ttgaagatga tcaagcaatc agtgacgaaa tacgtttgaa atatcgttat
    35881 ttggatctgc gtcgtccaaa aatgactaaa aacatcaaat taagaaatgc gacaacaaaa
    35941 gcgattcgtc attatttaga tgataatgac tttctcgata tcgaaacacc ttacctaggg
    36001 aaatctacac cagaaggcgc aagagactac ctagttcctt cacgtgtcca tgctggacat
    36061 ttctatgcgt tgccgcaatc tcctcaattg tttaaacaat tgctgatgaa tgctggattc
    36121 gaccgttatt atcaaatcgt tcgttgtttc cgcgatgaag atttacgtgg agaccgtcag
    36181 cctgaattta cgcaagtaga tattgaaaca acgttcttaa cagcagaaga aatccaaact
    36241 tacacagagg gattgattgc gaaggtaatg aaagaagttc gcggtatcga agtcacattg
    36301 ccatttcctc gtatgaccta tgatgaagca atggctcgct acggcagcga caaaccggat
    36361 acacgttttg atatggaatt gatcgatcta agcgatacag tcaaagaagt agaattcaaa
    36421 gtcttccaaa tggctcttga aaacggcggt gtcgtgaaag cattgaatgc taaaggcgct
    36481 gcagatcgct actcacgaaa agacatggat cagttaggtc aatatgtagg acaattcgga
    36541 gctaaggggc ttgcatggct gaaagtagaa gaagacggat tgaaaggtcc aattgctaaa
    36601 ttcatgggag aagcaacaga agcaatcatt aaagcaacag atgcaaaacc aggtgacttg
    36661 ctgatgtttg gcgcagataa aagtgaaatc gtcgctgctg cattaggtgc tatccggaca
    36721 cgtttaggaa aagaattagg actaattgat gaatcgaaat tcaatttctt atgggtaact
    36781 gattggccac aatttgaatt ttcagaagaa gaaggtcgat atgtctctgc tcaccatcca
    36841 ttcacgatgc caaaagaaga agatatcccg ttattggctg aagaccctgc aaaagtctat
    36901 gcagaagctt atgatatcgt cttgaacggt tatgaactag gcggaggttc tatccgtatt
    36961 catactcgtg agcttcaaga aaaagtacta gaaactctag gctttacaaa agaacaaatg
    37021 gaagaacaat ttggtttctt gttaacagct ttagattacg gattccctcc acatggcggt
    37081 attgcattag gattggaccg ttttgcaatg ttattagccg gagaagataa tattcgtgaa
    37141 gtcattgcct ttcctaaaaa cggaaaagca gcagatgtga tgagtgatgc accaagtatg
    37201 gtttcgcctt tacaattgtt cgaattgaat attgatgtaa cgaatataga agaatagccg
    37261 ataaaaaggt aaaaaggatt tgtcgaatac gcttcttttg acaaccttga tttaattgaa
    37321 aaacacttta taatgaagcc ataacatata aagcggggct tttccatgaa aaaaacattc
    37381 gaaaattggg cttatcatga ttacacgacc aagctttcgg tatctatcgt ttatgcgttg
    37441 atcgcatcag tcgcattgaa tttcttttat cagccaggac atatttattc aagtggtatt
    37501 actggtctag ctcagatttt gacgactttg tcaaaatcac tgataggatt cgaagtaccg
    37561 gtatcaacaa ctttgtattt actgaatata cctttgtttg tattggcgtg gttaaaaatc
    37621 gggaagaagt ttacaatttt cacttttatc acagtcactt tgacctccct ttgtatccat
    37681 attttaccgg aaacagtcct atctaccgat ccaatcatct gtgcgttgtt tgggggggct
    37741 gtgatgggac taggtattgg gctagcgttg agaaacggcc tatcatcggg cggtctagat
    37801 atcgtgagta tcacgattcg taaaaaaaca ggaaagtcga ttggttctat ttcgatctac
    37861 ttcaatgcat tgatcgtttt tgttgccgga attctatttg gctggcaata tatgttttac
    37921 agtacgttgt ctattttcgt cagtggaaaa gtaactgatg tcgtttatac taaacaaaag
    37981 aagatgcagg taatgatcgt aacgaaacat ccagaaaaag tagtaaatga aatccaaaac
    38041 tgtatgcgta gagggatcac gatcatcaat gaagcagaag gtgcttataa gcatgataag
    38101 cagacagtat tgatgacgat cgttacacga tacgaactgc ctctacttag agaagcaatg
    38161 aaagcttctg atccacaagc ttttgtaagt attgcagaaa acgtccagat acttggtcgt
    38221 ttctatgagg atggcattta gatcgagttt gacaaaaatt catgaaatac tgctgtagac
    38281 agacgttcgt ttgtctgcag tttttttcta ccatagtaga atcagcaaag aaaaactgct
    38341 tttttatttg aaattttctt aaagttgaaa aaaagcatgt ttattttttt aaatttatgg
    38401 gatgatagaa aaaaatagta gagaaaggac acgattctac ttgctatttt cacttaatac
    38461 ccgtatatta cgaatgaaaa aagtttatga aaagttggaa tattgaatgg aaaaacaaaa
    38521 aaggttaaaa aacagccgct acatcactgg gtttgatggg ctccgttcat tagctgtggt
    38581 gggtgttatc ttctatcacc ttctgccaac tagtatgaaa ggcggctatt tgggcgttcc
    38641 gatttttttc gtcgtatctg gttatttgat cacggatctg ctgcggcagg aatgggaaca
    38701 aaatggaaaa atcaatattt ggcaatttta tgtcagaaga atgaagagac tctatccggc
    38761 acttgttttt atgcttgtga cagcttcggc ttatatcact ttgtttcagc gagggctgtt
    38821 aaacaatctt cgaggagttg tggtcagcag cttactgtat gtcaacaact ggtggcaaat
    38881 caataacggt ttatcttatt tcgatcgatt tgctaatgag tcaccgttta cgcatatctg
    38941 gtctttagca gtagaaggtc aaaactattt ggtttggccg atcttgtttg tattgttgat
    39001 ggtatttgtt cgtaaaagaa aatggatctt ttatactgtg ttaggtacat ccttcctctc
    39061 cgccatcttg atgatgatct tgtatacacc aggaggcgat ccaacccgtg tttattacgg
    39121 taccgacact cgtctgtttt ctatatggtt gggaagtgcg cttgcgtttg tttggccaag
    39181 tacgcgttta aagaaaaaca tcccgaaaca agcgaaaaat attctgaata tcgctggagg
    39241 aatctcgctt ttggcactga ttctttgctt cttcttttta gatgatcatt acaatttcat
    39301 ctattacgga ggaatgctcc tagtgagtct gttatgcgta gtgctagttg cagtgacagc
    39361 acaccccgga gcaagtttga atcgatggct gacaaatcca gtatttactt ggattggcaa
    39421 acgaagctat ggcatttatc tgtatcagtt tccagttatg attttttatg aagcaaaggt
    39481 gactgatatt gccgatcatg ttttgttgca tactttagtg gaaatcacat tgattctaat
    39541 catcagtgaa ctttcttatc gatatgtgga gcgtccgcta gcacgttttg attatggtcg
    39601 tacattccaa gtgatgaaag gatggttcag caaaccgatc atcagtaaga gcaaaccatg
    39661 ggttgttcca ggcacattac ttgtagcaat tgcactcgtt ggtttcgtta cggcaccgaa
    39721 aaacgctgta acagccgatc aaaaacaact gcaggcaaaa atcgctgaaa gtaaaaaatt
    39781 agcagaagaa accagaaaca attcagaaaa caccactagt tcggttgacc aagcgatcct
    39841 tgataagtac gagttgtcca aggcacaagg gaataaggca caaaaattag aactaacggc
    39901 atttggtgat tcagttatgt tggatgctgc aacggatcta caagaacttt atccaaaagt
    39961 agtggtagat ggagatgtcg gacgacaatt atatgccagt gtaccgtaca ttgaaaaatt
    40021 aaaggaacaa gatttgttga aagacactgt tctgatcgga ctaggaacga atggctcatt
    40081 ctcggaagct cagtttgata gtgtgatgga tgcaatcggt gatcgaaaag tatactgggt
    40141 caatgtccga gtaccgacga agcgctggca aaatgacgtg aatgcgatgc ttgaacagat
    40201 gacgaaaaaa tacgacaata tgacgctgat tgattggtac agctacagta atgaccatag
    40261 tgactggttt tatgatgatc aagtccatcc aaatcctgaa gggatgacac attacattca
    40321 cttagtcagc gaagccttgc taggaggaag ttcaagtacg aacagcagtg agacaaacgt
    40381 gacagaatga tagctacaaa aaacttcagg caaaaacatt tgtttttgcc tgaagttttt
    40441 ttatatttac cgtgttttta acattcatat agattcagga agcaaaacgg ttgagtgtgt
    40501 ctgtaaataa cgttcttttt gttttcgggt gagttttttg aatgcagctt ctgcttttgt
    40561 tgcttcgctt cttgaagaaa atacttctgc atggatcatc ttaacaggtc ttcttttttg
    40621 aagacgagta tatttagcac cagtaccatt gttatgttcc tctaatcgtc tagaaagatc
    40681 ggttgtatat ccaccataaa atgtctgatc tttacagaat aatacgtaaa aataatgttc
    40741 atttgccata gagcatctca cgaatctctg cagtgtattc tccatccata ttgtaagtga
    40801 tcaatggtgg agcgactttg aatccgtcta atttgccttc tttgattcct tcaactaaca
    40861 aaatatttgc ctcttttcca ggttttgggt aaacaaattg gatcctttta ggtgcaattc
    40921 gatagtcttc catgacctga atgatttcta agaaccgatc aggacgatgg accatagtaa
    40981 agcgtccatt tgtttttagt agcttacttg aaatatcgat cacttcttct aacgtcgtat
    41041 gaatctcatg acgggcgatt gccaggtgtt tattagggtt cgttttattt gttggcagcc
    41101 ctttgaaata aggtggatta cagacgacta agtcacaaga attatgtcga acgacagata
    41161 agctgtcttt cagatcgatg gtgtggacag tcatttgttc atttaaatgg ttaagcgcga
    41221 tactacgtct tgccatgtct gctagacgag gttgaagttc gatagcgtcg atagccgcct
    41281 gggttcgttt acttaaaaaa agtccaactg cgccatttcc tgcacaaagg tcaacaatct
    41341 tccctttttt aggtacacgg gaaaagtgag caagcagcac agcatctaaa gaaaaagaaa
    41401 acactttgga actttggata atcttgatat cttctgcgta taattggtcg attcgttcgt
    41461 cttcgtgaag catggattcc ctccaaaatg aaaaaaatag tcgtctgtat tatactattt
    41521 ttattaaaaa gaaccaaggt ggcttgtcaa acctctattt tttatgcata ctatgaccat
    41581 aagaagggaa aggaagagac tatgttttat cgatttatgc gcggggtagt tcgagtacta
    41641 ttagccttag ttaatggaaa tgcccgctat gagaataaag aagttttacc gcaagatgag
    41701 aattacatac tagttgctcc ccatcgtact tggtgggacc cattatatct ggcggtagct
    41761 gcagcaccca aggaattcag ctttatggca aaagaagaac tgttcaaaaa tccagttctg
    41821 cgatttatat taactcgatc caatgctttt ccagttaaaa gagaaaatcc aggaccaagc
    41881 gtcattaaaa cacctgtaaa gattttgaaa tcgacaaatt tgagtttgat catgtttccc
    41941 agtgggacaa gacatgctac agaacttaaa ggcggaatgg cattgatttc cagaatgtct
    42001 aaagcgaaaa tcgtgccagc tgtatatcaa ggtccgttga cacttgggga tctgttcaag
    42061 cgtcagagag tgacagtgcg tttcggcgaa ccaatcgact tatcagatat caagaaaacg
    42121 gataaagaag gtatggcgga gatcgaacga cgaacacaag cagcttttga caaattggac
    42181 aaagaagtga accctgattt caaatatgaa gtcaaaaaaa aataaaatga taagtttatg
    42241 cgtgggcggt cttttgaatc tactgtcctg ttcagttcag actttttcaa tgaaaacgag
    42301 ggagagacgt caagtctgcc ctctttttgc gttccaatat gtaaaataga agacaggtgt
    42361 catgtaatgg atcaaagtgt tataataatt agaaggacat tttttgaggt gattgaatgc
    42421 gttttttaca tacagccgat tggcatatcg gaaaaaaact acaaggttat gacttgattg
    42481 aagaccagcg tcatgtacta tcttctatac tagatatcgc gaaagaagaa aaagtagagg
    42541 caatcgtgat tgccggagac ttatatgata ggagtgtccc agctgttgat gccgtagaat
    42601 tatacaatga attgatggtc gattggaatt taaaggaaaa gatcccgatt tttgctatat
    42661 caggaaatca cgatagcagt acgagacttt cagcgggtac gccatggttc agttattcga
    42721 actatttttt gcgaacacag ctagcagaag catttgaacc agtcgagttt aaaaacgtac
    42781 aatttttctt tcttccttac tttgaaccaa ttgcagcgag attatatttt gaagatgaca
    42841 atatccgaac gatcaagcaa gcggtagcca aggtgataga taaaatgcag gagctatttg
    42901 attcagaaaa aaaacaagta ttgattagcc atttttttgt aatgggaagt gtaaaatctg
    42961 attcggaaac gaaaatcgaa gtaggcgggt tggattcgat ccctggtgaa ctacttacac
    43021 cgtttgatta tgtagcacta ggacatttgc ataatcaagc tgctttgaga caggataatg
    43081 ctcgctatag cggttcccca ttgaaattct ctttatctga gatcaaccaa aaaaaaggcg
    43141 tatggatcgt tgacctgaca acaggcctgg agttgaattt tagggagatc gaacccctgc
    43201 atgatattga agaaataaca gcgagtttca aagaattgct ggatcctgcc ttttatcagt
    43261 ccgtcaatcg ggagaattac atctatatct atctcactga tagaacgatc atcccgaata
    43321 tgatgaacca gcttcggcaa atatatcctc gtattttggg gattgaacgg gcaaacggtc
    43381 gagcagaaca aacaaagcgc aaacgaaaag tactagaaat gaaagatcct caaaaattag
    43441 cagaagcatt ttttagtgaa atgacggaag agcagctaac tgcaaaccaa gaaaaatggc
    43501 tcggagaaac gttgcaagaa atcatggaaa tggaatgatc aagaatgaaa ccgaaaaaat
    43561 tacaacttaa aaattttggt ccttttatca atgagaccgt agatttttca cgtttgacag
    43621 aagcaccgct ttttttgatc agcgggaaga caggagctgg caaaacgacg atttttgatg
    43681 ggatcacctt cgcacttttt ggtgaaactt ccggaagact tcgctctgga aaagagatgc
    43741 gttctttgtt tgctacacca gacgaagaaa caagcgtgac tttttctttt gaacatcagc
    43801 agatgagcta tgaaatcgaa cgaaaaccag aacaagtttt agcaaaacga aaaggaaacg
    43861 gaacaaaaaa acaggcagcc aaagtaacgt tgaccatttt tgatgggaaa ggaaaagaaa
    43921 taaaacaaat cacaaaacgt acagaagcag atcagttgat cagagaactg atgaatctcg
    43981 atgcgaaaca gttttctcaa atcgttttac taccccaagg agaattccgt aattttttgg
    44041 tttcatcaag cagtgataaa gagactgttt tacgtcattt attcggtact caatttttcc
    44101 aacagttcaa tgagcgatta aaagaaaaag caaaacaaca gcaacaaaaa ttggatcatt
    44161 tagagcaaga gctgcatctt ctgcaaaaaa ggtttgtttt agtcaaagaa gaggaaccag
    44221 cagcagggtt tgaagccgtt ttagcccaat ggacaaacca tcaggcaata ctcgaaaaac
    44281 aaatcacgga agaaaaagaa gctctaacgg aacttcagga aaaacaaaag caactagaaa
    44341 attcctacta tcgttttgaa agcttgaaaa aaagttatga agaaaaaacg atattaataa
    44401 acaagcaaaa ggaattagct gctcaaaaag acgaaataga ggaaaagaaa cgatggattg
    44461 ggtattatga atttacgcaa cgattagtcg aaccaatcca gcgcctacaa gaaataaaag
    44521 ttgaaaaaat agaactgtca caaaaaagta gtgagcaaac aaagaaacta gcgatgacta
    44581 cgcagaatta tgaagagtgg caaaaaaatg aagcaaaccg cgtacgccga caaacaaaaa
    44641 tcaaagaagc tgctcaaaaa ctgcaaaaca aacagatgat ggtgccaatt gtcgaatcct
    44701 tacgtagtaa aaaacaagac gcagaacaat taacaatttc attggataaa gaagaattga
    44761 atgccgagaa gctgtatcaa aatctcgcta tctatcagga aagaactcga attcttcaag
    44821 aaaatttact ggatcaggaa aaactgcaag aagatcgtct tgcttttgga cagttaaaat
    44881 atctagatga aaagcagcaa gaaatagaaa aacaaaaagc aaaagacact gggtcgctcg
    44941 cacagataag agaagaactc aaaaaagcaa ctgaagaaat aacaaaaaat agtcagctat
    45001 tgaaagaaca gacagaacaa gcagaacagc tgaaaagcca atgggcaaag cagcaaattg
    45061 ctcgtcttca gctttttttg cgtccagggg agccatgtct tgtttgtgga tcaacggaac
    45121 accccataca acaattaaaa cacgaacagc caaataagga agatatctta aaagtagaac
    45181 aacaactggc agaagctgag tctgctgtag aaagaactca gcaaatcatt gccaaaagtg
    45241 aagcacatga aaataatctt caacaacaat tatcagattt agaacaaaaa atcatggcaa
    45301 cgaatataca attggctgaa caagaagaac acttgcaaca ggctttgaaa aaggcatttc
    45361 ctgagataac agaacaaaaa acaaaaacaa ctcttgtagt aatcgaacaa tctttagata
    45421 gacgaaaaag caagatggcc aatgcaagaa tagagctgga agaaataaga caacaagcag
    45481 aaaagctcga acaacaatta gctgtctaca aacagcagca gacagaaaaa aataatcagc
    45541 tccagcaact gcaaggagaa atcaaaagtt tagaacaaca attgcaagat acggatagta
    45601 actgtttaga agaggagata cgacgacttg aaaaacagtt agaagaagac tcgaatcaac
    45661 tagctgttga gcagaaagca ggagtgcaat tgttgcaaga tcttcatacg cttcggacac
    45721 agcttgatct tcaacatgaa caaatagaaa aaatagatga aaaaatagaa aaggaacaag
    45781 aaagaatagc taaaaagtta gaaagccagt cgtatttttc aacaattgaa gaaatctgtg
    45841 catttgcaca acagcaagca caatataaag aaaaccaacg tacagttact tcttatcatg
    45901 aagagcaatt agtgacagct gaccggctgc gtcaattaga aaatatccag ctaactgaga
    45961 cattcccaga tacagaagag atcagccaac agttagaaga aataagacgt atgatcgaaa
    46021 taaagcaaga aaagatatac ggtttacaag aacaaaaaat tagtaatcaa aaaatctacg
    46081 atgaatgtct ttcgatttat cacacaagcc agacgaaatt agatgagctt acgcaattac
    46141 agcagctttc gcaaacattc aacggagaaa atcctaagaa gactagtttg gaacggtatg
    46201 tgcttcaggt ttatcttcaa gaagtgttac aagtagcaaa tgatcatctc caacgtttaa
    46261 cgaaaaatcg ttatcaattt gagctagcgg ataccattgg cagttatcga gggaaaaccg
    46321 gattagaaat caatatctat gatgatcagg caggtatgtc tcgaagtgcg cataccctgt
    46381 ctggcggtga gagttttatt gcagcacttt ctttagcatt agctcttgcc gatgtgatcc
    46441 agacacaagc tggtggtgta gcaatcgatg cattgtttat tgatgaagga tttgggtcat
    46501 tagatgaaga agctttggaa atggcaatgg aagctttaga aacgatcgaa agtgaaggaa
    46561 gaatgatcgg catcattagt catgtaagag aattgaaaga acgagtatta caacaaataa
    46621 gaattgatac agaaggtagc ggacaaagca aaatacgcta tcaacttgga taaaaaggga
    46681 gattggtaac tatgaaatgg agtattccag aaaaagtcat tgcacgtgga cgtcaatact
    46741 tgcaagaagg ccgagtgtta tcggttgtcc ctgaccaaga agaaaacgtg tggcatgcag
    46801 aagtcttggg cagtgaattg tatatggttg atttggatgg tacagcaaaa gagatagatt
    46861 actgccaatg tccctattgg gaggaacata aatattgtaa gcatacagtt gctgtggaac
    46921 tttatttacg gtcgaaaaat ctatctcgta tcatgaaaaa agaccagcca atgctagaac
    46981 cggtaaaagc aacaagtgag ggagaaatca ttgcaaaagg tttttctcgt ctaaaaacac
    47041 aaaacaatcc tcaagcggtg caacctttga ttattgaata ccaagtagaa acgattgaga
    47101 ctaatcagta tcacccagag ctttctattt tggcacttta cttgcgtatc ggttcattgg
    47161 catcaccgaa aaaaacatat atcgtaaaaa acatctatga ttttttacaa gcttacacag
    47221 aaaaagaaac ttacactgtt aataaacagt accagtttcg tctagataaa cgggcgtttc
    47281 aaaaagaaga tcaaaaaata ctcacgcatc ttgcaggaag tgctcagacg catcaattat
    47341 taggtacgaa cggcttacaa gtcaaaggaa aactagataa aagatatctg cttctcccag
    47401 tagaacaagc ccgcttcctg ttagagcaga tgaatcagac tacacgtttg catctgcaga
    47461 taaatgaaca aaacgatcaa ggaatacaat tttccgatca aaacaaacct ttatcattca
    47521 aagtggaaaa atttggagaa gattatttgc tgcaagcatt gaatgatttt gattttttct
    47581 ttatgcatta tcagtgggga gttatccaag ggacatttta ttctctgacg aaatatcaac
    47641 aaacggttta tcaaacttgg aaacaattga ttcgccgatt ggagaaacca gaagtagtgt
    47701 tcaagaaaaa agaattaccg tctttgttta aagagattat ccctctgctg tcagagatcg
    47761 gcgacgtagc tattgcccct gcagtagaag aggaagtagc agatatcccc gtcgaattta
    47821 ttttcttttt ccgaaaagca aaaggaaaaa tccaagctcg agtcgatttc gtctatggcg
    47881 aagtcatcta ttcgactgac ccgaagcatg aggtctcgac agaacaggct tttcaggtgt
    47941 tacgtgacac agtagaagag aaacgagtac ttgatctatt cagaatgtat cgctaccaga
    48001 aaaatgaaac tggttatgaa cgtccattgc caagcgggga agaactatat gcctttttcc
    48061 gcacagaact atctgtcttc cgtcagtttg gtgaagtcag acttggaaga aaattgcgag
    48121 agttgtattt agatgcgcat aaacatcaac cgaaaatcga agtaaccgag accggttcat
    48181 ggctcgatat tcgattcgat gtgacaggga tagaggagca agaaatcgat catgtcctgc
    48241 aaagcttgct gagaaatgat gctttttata cattagaaaa tggtcaggtt ctttcttttg
    48301 attcagaaga atttcaacaa acaagccaag tgttgcaaca attacgggaa tcgatacgta
    48361 cagaagaagg aacgatccat gtacctaaaa atcaaggatt gatcattcaa aatcaattgg
    48421 aaaaaagcaa tgctactttt agtgaatcgt tccaaacgat ggtacaagat ctcattcatc
    48481 cagaaagata ccaggcacag cttcccaaag gattgaatgc aacgatgaga gattatcaaa
    48541 agcaaggttt tcgatggttg aagatgttag gtcattatca gtttggcggt attttagccg
    48601 atgaaatggg acttgggaaa acactacaga caattgcctt tttactttcg gaaaaagaag
    48661 aacggaaaag cttctctgct ttgatcgtag cgcctgcgag cttaatttac aactggcagg
    48721 cagaggtgag aaaattcgcc ccttctttaa gtatccaagt aatcaatggg aataaaaaag
    48781 aacgtgaaga attactggct aaagacacag atatccgagt gacttcttat gccagcttaa
    48841 gacaggatct agctaattat cagtcgcaaa aaattgatta tttgattctt gatgaggcac
    48901 aaatggtcaa aaatagcagt acgaaaactg cacaagcatt gagagaatta gctgtacctc
    48961 agcggtttgc actgagtggg acaccaatcg agaataattt agaagaattg tggtcattgt
    49021 ttgcaacgat tatgccagga tttttcccga ctaaaacaaa attcagggaa cttgccgcag
    49081 aagaaattgc gcaaatgatt cgaccgttta ttttacggcg agataaacaa actgtgttga
    49141 aagacttgcc ggaaaaaaca gagatgaatc tatattctgc acttacagaa gaacaaaaaa
    49201 ctgtttattt agcttacttg cgtcaaatgc gtgaagaaat cacatcaatg gattctgaag
    49261 cattcaagaa aaaccggatc gggatacttg ctggtctgac aagactgcga caaatttgct
    49321 gcgatcctcg tctgtttatt gaagattacc aaggcggttc cggcaagctc gaacaagtaa
    49381 aagatctact ggtagctgcc aaagagaaca aacgaagagt tttgctgttt tctcaattta
    49441 cagggatgct gactatcttg caagaagagt tagccgagct agggatctcg acattttatt
    49501 taagaggcag tacgaaacca caagatcggt taagtatggt agacgcattt aatgctggcg
    49561 aaaaagatgt ttttctgatt tccttaaagg ctgggggaac tggattgaat ctcacgggag
    49621 cagatacggt catcttgtat gacctttggt ggaatcctgc cattgaagaa caagctgctg
    49681 gacgtgcaca tcggatcgga caaaaaaatg tggtagaagt gtggcgtatg atctctgaag
    49741 gaacgattga ggaacgcatg gattcactcc agcaagaaaa acgcgaactg ttccaaaaag
    49801 tgatccaagg taatgaagaa cagttgacga aactgactga agaagacatc cgcatgattt
    49861 taagtgtagg agaaatcgag gagtaagaga ctcgaaaaag cgttcagtaa tgggtaagaa
    49921 agcctttcta aagaagcgat tgtttatttg atttaaaaaa tagcaaggag gctgggattc
    49981 cattttatcc cagcttcctt gctatcattt ttttatttct atttttaaag gttatacaaa
    50041 gattgtcttc attgtctcta acagctgatc aaaagaagaa atctcgatat cgcaaattgg
    50101 agtctgttca attttgcgtt ctcgatggtt gaaccaaagc gcttgccagt tagcactttt
    50161 tgcacctaga acatcattgt cgtaattatc gccaacgtat aaggtctctt ctggaagcat
    50221 atgaaattct ttctcagcta gctgaaaaat ctctttttct ggtttttgga aacctgttgc
    50281 ttgagaaatg agcatattgc gggaaggaat ccaattgttc aattgaagtt gcttcagttt
    50341 ttttgtctga tggtcagtcg gcccatttgt gataattcct aatgggacat tcttttcctt
    50401 caagaaatcg agggtttttt tgacctcctc atgcaagcag atattatcaa gttcttcctc
    50461 gtaaattttt tgaaataaga ggccatcttc ttccgtaata tgagggtagt ctaaatcttt
    50521 tagtgattgg ctgatccgat gtgctcgcat atattctagt gtccaatctc ctgccattac
    50581 ttttgggaag ttttcatcac tatgatggcg gaaccggata tatagtggat gcatatctgc
    50641 atctgaaaca agcggaacga ctcttttgac cgcgtttcga aatggctgct gctgatcata
    50701 catcgtatca tcaacatcaa acacaactga tttaatcaaa attttacacg cctttcggtt
    50761 ttcattgatt ttttcacaaa tccttgacta ttgtaacaga aaaaattgtc taaaaaagct
    50821 gataaataaa ggtttttctt actatatttc tatttccttg acagaataag gtttttatat
    50881 ttcagttttt ttcactaaaa atcgtgaaac acgtcaataa gtttgatgaa caaattaatc
    50941 attatggtag aagtcgattg atttatttta atgcaatctc tgtggaattt tcgaaagatt
    51001 tattttt
//
LOCUS       DACCXO010000009        47346 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.009, whole genome shotgun sequence.
ACCESSION   DACCXO010000009 DACCXO010000000
VERSION     DACCXO010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.009"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(53..787)
                     /locus_tag="IVY70_000465"
     CDS             complement(53..787)
                     /locus_tag="IVY70_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP7957625.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="IVY70_000466"
     CDS             1114..1524
                     /locus_tag="IVY70_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP7957626.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="IVY70_000467"
     CDS             1517..2218
                     /locus_tag="IVY70_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP7957627.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="IVY70_000468"
     CDS             2218..3804
                     /locus_tag="IVY70_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7957628.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="IVY70_000469"
     CDS             3823..4044
                     /locus_tag="IVY70_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP7957629.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="IVY70_000470"
     CDS             4041..4802
                     /locus_tag="IVY70_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP7957630.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="IVY70_000471"
     CDS             5182..5691
                     /locus_tag="IVY70_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP7957631.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="IVY70_000472"
     CDS             complement(5766..6305)
                     /locus_tag="IVY70_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP7957632.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IVY70_000473"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IVY70_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP7957633.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7372..8169
                     /locus_tag="IVY70_000474"
     CDS             7372..8169
                     /locus_tag="IVY70_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP7957634.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8197..8832
                     /locus_tag="IVY70_000475"
     CDS             8197..8832
                     /locus_tag="IVY70_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP7957635.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="IVY70_000476"
     CDS             8852..9625
                     /locus_tag="IVY70_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7957636.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="IVY70_000477"
     CDS             10033..10830
                     /locus_tag="IVY70_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP7957637.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="IVY70_000478"
     CDS             10808..11221
                     /locus_tag="IVY70_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957638.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="IVY70_000479"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="IVY70_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP7957639.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="IVY70_000480"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="IVY70_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP7957640.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="IVY70_000481"
     CDS             15998..16558
                     /locus_tag="IVY70_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP7957641.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IVY70_000482"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IVY70_000482"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP7957642.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="IVY70_000483"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="IVY70_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP7957643.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="IVY70_000484"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="IVY70_000484"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP7957644.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="IVY70_000485"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="IVY70_000485"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP7957645.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="IVY70_000486"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="IVY70_000486"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP7957646.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="IVY70_000487"
     CDS             21905..22483
                     /locus_tag="IVY70_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP7957647.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="IVY70_000488"
     CDS             22535..23017
                     /locus_tag="IVY70_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP7957648.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="IVY70_000489"
     CDS             23134..23508
                     /locus_tag="IVY70_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957649.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(24186..25538)
                     /gene="brnQ"
                     /locus_tag="IVY70_000490"
     CDS             complement(24186..25538)
                     /gene="brnQ"
                     /locus_tag="IVY70_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP7957650.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            25685..26275
                     /locus_tag="IVY70_000491"
     CDS             25685..26275
                     /locus_tag="IVY70_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP7957651.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(26403..27752)
                     /locus_tag="IVY70_000492"
     CDS             complement(26403..27752)
                     /locus_tag="IVY70_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7957652.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(27920..28660)
                     /locus_tag="IVY70_000493"
     CDS             complement(27920..28660)
                     /locus_tag="IVY70_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7957653.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(28673..30265)
                     /locus_tag="IVY70_000494"
     CDS             complement(28673..30265)
                     /locus_tag="IVY70_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7957654.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(30833..31129)
                     /locus_tag="IVY70_000495"
     CDS             complement(30833..31129)
                     /locus_tag="IVY70_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957655.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            31305..32030
                     /locus_tag="IVY70_000496"
     CDS             31305..32030
                     /locus_tag="IVY70_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP7957656.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            32023..32865
                     /locus_tag="IVY70_000497"
     CDS             32023..32865
                     /locus_tag="IVY70_000497"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP7957657.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            32868..33389
                     /locus_tag="IVY70_000498"
     CDS             32868..33389
                     /locus_tag="IVY70_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP7957658.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(33642..34205)
                     /locus_tag="IVY70_000499"
     CDS             complement(33642..34205)
                     /locus_tag="IVY70_000499"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP7957659.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            34456..34725
                     /gene="rpsO"
                     /locus_tag="IVY70_000500"
     CDS             34456..34725
                     /gene="rpsO"
                     /locus_tag="IVY70_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP7957660.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            34913..37039
                     /gene="pnp"
                     /locus_tag="IVY70_000501"
     CDS             34913..37039
                     /gene="pnp"
                     /locus_tag="IVY70_000501"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP7957661.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            37549..37815
                     /locus_tag="IVY70_000502"
     CDS             37549..37815
                     /locus_tag="IVY70_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7957662.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            37959..39950
                     /locus_tag="IVY70_000503"
     CDS             37959..39950
                     /locus_tag="IVY70_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP7957663.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            40180..41481
                     /locus_tag="IVY70_000504"
     CDS             40180..41481
                     /locus_tag="IVY70_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP7957664.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(41611..42981)
                     /locus_tag="IVY70_000505"
     CDS             complement(41611..42981)
                     /locus_tag="IVY70_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP7957665.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(43163..43486)
                     /locus_tag="IVY70_000506"
     CDS             complement(43163..43486)
                     /locus_tag="IVY70_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP7957666.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            complement(43811..44419)
                     /locus_tag="IVY70_000507"
     CDS             complement(43811..44419)
                     /locus_tag="IVY70_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP7957667.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            44749..45465
                     /locus_tag="IVY70_000508"
     CDS             44749..45465
                     /locus_tag="IVY70_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP7957668.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            45478..46173
                     /locus_tag="IVY70_000509"
     CDS             45478..46173
                     /locus_tag="IVY70_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP7957669.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            46257..46886
                     /gene="udk"
                     /locus_tag="IVY70_000510"
     CDS             46257..46886
                     /gene="udk"
                     /locus_tag="IVY70_000510"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP7957670.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
BASE COUNT        15352 a         8766 c         9852 g        13376 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcacagcatg acagcattgg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ctagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagttttt ttgcgaaaac tatttttata tcatatatat gtctaaaatc tatattataa
    23881 aagaaatgtt gtttgatcag cttctcttcg gtattagttc aaaacaaaac ggcaaagtat
    23941 atggagctac tttgccgttt tgtttctcta atactcaggt cagtttatag acgctcaata
    24001 aacggtgttc aaaagccagt ttctcggtat taagtcaaat ttatgcaaaa atgtgagaag
    24061 ccattttcaa aaaatatttc ttaatactcg aaactgagcg gctttttcac gacctctcaa
    24121 taaacagtgt tcaagagaca gcctatcggc aataaggctg aacgtctctt tcacaacctc
    24181 ttttatcaat ctgctacttc ttctttctta gcgaaactcc aaatcagtcc gataacaaaa
    24241 ccgataactg ctggtacgat ccaccccatc cccaatttga aaaacggcaa gtagctttcc
    24301 gcaacatata aaagattttg aacaataggt gtttgtttga tagtttctgg gcaagcgttc
    24361 aacccatcaa aaatcgaagc aatcaatgta aaataagtag tcattcgata aacagccgta
    24421 cgctgcttaa acaaaggacc aactaacact agtaaaatca gtgtcattgc taatggatag
    24481 atgaacatca aaacaggcaa cgaaaattga atgatattcg tcaagcctac gtttgccgct
    24541 aaacaaggaa ggatactagc aagtgcaaca aagaatgcgt aatttctttg cggaaacaaa
    24601 tctgtgaatg tttctgcaaa agctgtaatc aaaccaatac cggtcttcaa gcaagctaat
    24661 ataacgataa atgctaataa gatactgcca taagtaccta aataatgatc agcaatctga
    24721 gccagcgtga tcccgccatt ttcacttaga gcaaagctac ctaagctcat agcacctaaa
    24781 taagcgagca aagtataaat gattcccatc aatataatgc tgatcgcacc agatttgatc
    24841 gtgtctttag cgatttcagc aggttttgtt acacccatgt tttggattgt tgtgacaatg
    24901 atgatcccaa atgctaaagc agcaagcgca tctaatgtgt tatatccttg agtaaaccct
    24961 gtgaaaaaag catgctcttg ataatttggc tgaataggcg cttgatttac agaacctagt
    25021 ggattcaaaa aagcaaggag aagcaaagca cctaataaaa ccaaaaatgc aggattcagg
    25081 aactttccaa cataatctaa tatctttgtc ggttttcttg aaaaccacca agccagcaca
    25141 aaaaatacaa tactgaaaat tgctagatat agcgtctgtc tgctaggatc gataaatggt
    25201 gctaaaccaa tctcgaaaga tgtggttgca aggcgaggta aagcaaaaaa cggtccgata
    25261 actaagtaaa gcaagatggt aaaaaccatc gcatatgttt gatttatccg ttttgctaat
    25321 tcatatacac ctgagctttt cgatacacca atggcaatca cccctaagaa gggaagaccg
    25381 ataccggtaa ccaagaaacc tagattcgcc caaaaggtag cagcgcctgc ttcttgaccc
    25441 aaatgaacgg gaaagataag atttccagca ccaaaaaata gaccgaaaag catggagcct
    25501 ataaaaagat agtctttaaa tgataatttt tttgtcatac ataactcctt tacggttttt
    25561 ttaaaagtaa caaaatttcg aaaaatatgc aacaattttc agaaaatttc ctatataaaa
    25621 cgacggttat ttaataacgt aagtgataaa ataaaagcgc tgaatcatct gaggaggaat
    25681 catcgtgaca aagtcgagat tagaagcatt tactgatgga gtggtagcca ttgttttgag
    25741 agttcttgta ttagagatca agatccctaa tacatctagc tttcattctt tatgggtcat
    25801 ccgaaataca ttattagctt ataccatcag ctttatattt gttgcggtga tctgggtgaa
    25861 ccatcatcgt atatttcaga tggcagaacg aattaattac cgagtggtct ggtcgaatat
    25921 ctttttgctc ttttggctga ctttatgtcc ttctgttact agttgggtcg ggcgtaaccc
    25981 tgaagctttt tggcctgaga tggcttatgt cttagtatac acgatgtgga gtttttcttt
    26041 tggtatcttg atgaggcaaa tcatgaaagc aaataaacca gattcccatg tcgtgagagt
    26101 tttgtcaaag gaccgtcgaa gtcttatctc catgatgatt aacttatgct tgattgccgg
    26161 tgtctttttt gtaccgttga tcgggttatt cggacgtttt tttgtatctg gtatttggat
    26221 tttttcctat aaaaaagcag atgcctatta tcagcggttg tttccgggga aatagaaaag
    26281 aggctgggac agaagctttt aactccaaga aataagccga aatttcacaa aaatttaaga
    26341 agcaattttc gtaaattcct tcttatttct cgaagctgac cacttctgtc acaactactt
    26401 tcttacttct tacgttcata tttcatatga tagattagta atagaagcaa gaaccacaca
    26461 ggtgttagta ataaagcgat tctcgtatct tttcctagga acaagacgac acacacaaag
    26521 gccatgtaaa gaaataccag ataattacta aatgggtaca acggcatttt aaatgaatga
    26581 gcttttttgc cgaggatact tttacgatat ttcatgtgcg tgtagacaag catactccag
    26641 ataaacaaga aacaagtagt ggcaatactg cttactagtg tgaatacttc actaggcatc
    26701 acgtaattca agataacaga aatgaaaatg acgatagtag aaaatgtcaa agcattccct
    26761 ggcacatgat gtgttgttaa ctttttgaat tttttagggg cactgccttc ttgcgctaaa
    26821 gaacgaagca tacgtcctgt actatatatt gcactgttgc aggcagaagc tgctgcactt
    26881 aatacgacta aattgatcaa ggaagcagca atcgtgatcc caatttccga aaatacttct
    26941 acaaatggac tgttatctgg attcaagctg ttccatggat agattgccat aatgacaaaa
    27001 agagatccta agtaaaataa gatgatcctg ataggaatat tattgatagc ttccggcaac
    27061 actttttcag gattttctgt ttctccagcg actaatccaa ctaattctat cccagcaaaa
    27121 gcaaaggtta ccatttggaa agctaagata aatccttttg caccagttgg aaagaatccg
    27181 ccatgagacc atagattcgt gatactggct gtgccaatat ctgtcgtata gtgggtgagg
    27241 atcatataag ctcctacgag gataaaggca atgatcgcta caactttgat caatgcaaac
    27301 caaaactcta actcaccaaa caatgagaca gccaccatat tcaagcaaag aagaagacct
    27361 aatgcaatca gttcaggaag ccactgagga acaccaggcg cccagtagcg tacatacatt
    27421 ccgatggctg ttaaatcggc cattgcaatc gatacccagc agaaccaata cgtccagcca
    27481 gtgatgaaag ccgctttttt gccaagatat tctgcaacaa aatccagaaa agaatggtta
    27541 ttggtgtttg ataacagaag ttcgcctaac gcacgcataa tgaaaaaaca gatgcatcca
    27601 gtaataagat aggcgagcaa tatcgaaggg ccagctagct ggatagactt tccggaacct
    27661 aaaaacaacc ccgttccaat cgctccgcca atagaaatca gttggacatg tctatttttc
    27721 agtccacgct gcagttcttg ctcttcattc atttttaaat catcctaact aaaaaagttt
    27781 aattacttcc atattaaaag agttttctga aaaacacaac taagattcca aaattttcag
    27841 aaatttacct tttatgagta attcataaaa aagaactgcg gtaaacaccg cagctctttg
    27901 tcccaatcag ctattgatcc taagcattta aaatacgtga aaggaattct tttgtacgtt
    27961 cttctttcgg atggacaaag atatcttctg gtgtaccttc ttctgcaatc acacctttat
    28021 ccataaaaat gacacggtca gatacatctt ttgcaaattc catctcatgc gtcacgataa
    28081 gcattgtcaa gccagtatgt gctaagtctt tgatcgtatt aagcacttcg ccaaccattt
    28141 cagggtctaa cgccgaagta ggttcgtcga atagcaatac atctggattc attgataatg
    28201 cccgagcaat tgcgacacgc tgtttctgtc caccagataa ttgtgctggt ttggcttcga
    28261 tataacgatc cataccaact ttttccagat tttcgattgc tacttttttg gcagtttctt
    28321 tatctcgttt caggacagtt gtttgaccag acatacagtt ttccaagaca ttcatattgt
    28381 tgaacaggtt gaaggattgg aaaaccatac ctagattcgt tcgatattta ggtaaattat
    28441 atttaggagc taatacattt tctccattat aaatgatctc gccgcctgtt ggtttttcca
    28501 gtaaattgat acagcgcaag aaagtagatt taccagaacc ggaagaaccg atgatcgtca
    28561 caacttcacc tttattgact gttacgctga tatctttcaa tacttcattt tcaccgaagc
    28621 tttttcttag atgtttgatt tcaataattt tttcattcat gattcttcct ccttattcct
    28681 gcaaatttga attgtcgatt tcttcaagct tggtataagc tgaaggacca tcgattttct
    28741 tttctaccgc ccgcaggatt ctggtgatcg taaaggtcat gactaagtag atgatcccga
    28801 cgatcgtaaa tgtttggaag aattggaaat tcgcaccgga agcagagttt ccttggaaga
    28861 acaaatcagc tacgccgatt acacttaaaa ctgctgtatc tttgatattg ataacaaact
    28921 catttcctgt tgctggcaag atattccgta aaacttgtgg aataacaact tttctcatcg
    28981 tttggccatg ggtcatacca atggcttgcg cagcttcgaa ctgcccttca tctactgcaa
    29041 aaatcccccc acgaacgatc tctgtcatgt aagctccagt attgagagag acaataaaca
    29101 aagcggcaat cgttcggttc aatgaaatgc caaaagcgag tgccagaccg tagaagatga
    29161 ccattgcttg taccatcatc ggtgttccgc ggaatacttc gacatagata gaaagaagtg
    29221 catttccgat tttttggaag aagcgagtca gtgcatttcc tgattcaggt accgaacgga
    29281 agacaccaat cagtaatcca agtgtagtac cgacaactgt cccgatcaat gcgatgaaca
    29341 aggtaagtcc agcacctcgt aagaacatat tgccgtattg tttccaaatc gtagtgaaat
    29401 cagaaatgat gccaggtttt tcttcactat cagttgcagc aggctgatct ttgactgctt
    29461 ggtccataat tttggttcgt tcatcgttag agatgccaga aagaatctgg tttacttttt
    29521 ggatatccgg gtcgccttta cgcataccaa cagaaatctg aacatcttcc gggtttgtct
    29581 ggaagccatc tgcaccgctg aattcaagca tttccaagtc tttgttcaca ctagttgccg
    29641 ttacaccttc cggacgttcg ctgacataac catcgatcat tccagaagct aaagctgttc
    29701 tcattgctga gaaattatcc attgcttgtt gtttctgcac atctggtatc tgatcgatca
    29761 cagtatagtg gaatgtattt aactgagcag taatctttgc tccagaaaga tctgctaaac
    29821 tttttgcttt tgcatatttg ctatcttttt gaactactac tactaattgt gattcgtaat
    29881 aaggatcggt aaaatcaatt tcttttcgtc gttcagcagt agggctcatt ccggcgatga
    29941 tcgcgtcgat cttgcctgat tgaagggcag gagccagacc atcccatttc gtttggacga
    30001 tgactaattt tttccctaaa ccatctgcta cttttttagc gatttgaaca tcataaccgc
    30061 cggcgtaact attttggcct tgaattggca aggcattatt ctcatctgtt tgttgagacc
    30121 agttgaaggg tgcataccca gcctccatac ctacgcgaaa ctcatcgctc ggtgtttttt
    30181 catctgcatt tgccagagat cccacactga atagtgagaa gacaaaagca aatagtacgg
    30241 caactgaaac catatttttt ttcatttttt tctccctttc tgattatatg atacacgagc
    30301 ggtctagatg aaatgcggta aagctttatc tgaaaaaata aaaagcgacc gatttataga
    30361 tataaaaagg ccgctaaaat tccataaggt gataaacctc tcacaaaaca tagcgcaact
    30421 tagcatcact gctaagacag tccgcaagct cttaacttgc gtcccaacat acactccaag
    30481 caaaaaagtg cacatttcgg cgatcctccc tattcctgct tctccatgtt tgcttcactc
    30541 cacagggtta atgattctcg cgacctctac ctcattgatt gaggcatctc gtattcagtt
    30601 atttatacaa aaaaagttta cgatactcga gaggctttgt caatctataa ctgctgtttt
    30661 ttgaaaaaat actgataaat atagaaatct ttacttttac cgaaaaaaca atcaaaatgt
    30721 gaatcatcta tccattgcaa acgatagtat aattatacac aaaaatgtat gtaatacaat
    30781 aaaaaaagaa ctagaacaga aaatttcctg tgtccagttc catctcgatc atttaagctg
    30841 ccgctttctt tggctcacgt cctaggatcg cttgcaaaag aacaacgacg ccataaatgg
    30901 ctaatattag ataaatcaaa cttgagtatt gggtgattcc ccagccccat gttcgatata
    30961 cggctaatgt aatggctaaa acaattgcgg caatcacgtt gaataaagca aaagcccaaa
    31021 gatttccatt ttctttgcgt acaagataaa aaacagagaa ataaacgctt aacgctaacc
    31081 aggcgataca agccagaata acgccccaat aaataaagaa tggtaacata tcgattcacc
    31141 tcacttttat acagcagtat tgtaacacgt tttcattctt ttgtcagtga acattgtgat
    31201 aaattgttca aatgcccata acactttttt attcccattt ttctgacttt tcaacaataa
    31261 atttgttcga gtatgggcta taatggaaag tgaggtgaca aaacatgaaa caattaacag
    31321 tagacgaagc agcaaaagag atattagctt atcagcaaat tacatttttg accggtgcag
    31381 gggtatccac accttctgga gtgccagact atcgatcttt gtcaggaatc taccacggga
    31441 tggagcagcc ggaatatttg ctgagtcatc aagcaatggt gaatgaaccg gataaattct
    31501 atcaatttgt caaaaagatt tatcatcctg aagcaaaacc caatatcatt catttggaaa
    31561 tggcacatct agcgaaagaa aaaaatgttt gggttgtttc ccaaaacatc gacggtttgc
    31621 acgcaaaggc taaaagcccg caactcgtca attttcatgg aagtttgtat caatgtcatt
    31681 gtcgaaaatg tcaacaaacg gtagactgga aagaatatct tcacagcgac aaacatcaag
    31741 catgcggagg acaaatacgc ccagacatcg tgttatatgg tgaagggttt caagatggag
    31801 tcatggagca ggcggcttat gccgtcagcc aagcagagct gatcgtcatt gttggaacga
    31861 gttttcaagt tcatccgttt tgtgatctgg tacagtttcg ccagccgtca gccaaaatct
    31921 tagtaatcaa tcaaacgcct gtttatttag cggagcctta tacgtttatt caagaagacg
    31981 gagcggttgt tttcaaaaaa atacaagaat taggagcaga ttatgactga attcaaaacc
    32041 gaaaaagaaa aaatgattgc aggcgagctt tattttgcca atgatcctga gctggttgcc
    32101 gaccgtatgt ttgcaagaag tcaaagccaa atcatcaacc aagcagaatc agcggaactg
    32161 cgatcccaat tgttgaaaga gacttttggc cgtacaggga aaaaaatcta tatggagcca
    32221 gttatcaatt ttgattacgg atataatata tttgttgggg agaattttta tgcaaatttc
    32281 aattgtacat ttttagatgt cagcacgatt gaaatcggtg ataactgcat gttcgctccg
    32341 aatgtccagc tttacacggc gacgcatcca ctacatccag tgaaacgtaa cagcggtctg
    32401 gagtacgcaa aacccattaa aatcggtaat aatgtttggt taggcggagg agttatcgtc
    32461 acgccgggtg tcactctagg ggacaacgta gtagtcggcg caggttcggt tgtgaccaaa
    32521 tcttttcccg acaatgtagt gattgcagga aatcctgctc gtatcatcaa gacagtagag
    32581 gaagaactaa cagaagagtc tcttgaaaca ttacgtcata agatcgatat gatcgatcga
    32641 gagttgattg ctttattaga aaaaagaatg gatacggtaa ctaaaatagg acaagtgaaa
    32701 aaagaagaaa accaagcagt ttatgatgca aaaagagagc agcaagtatt ggataaagtt
    32761 gttagtctgc tgaaaaataa gggatataag gaaacaataa cagatacgta tgttgatttg
    32821 atgaaacact cgcgcgaata ccaaaacaaa atgaaggagg aataagtgtg atcgaaggag
    32881 agacacggcg aaaacaaatc atgcaaacct tggcaaatac agaaaaacca gtgagtgcta
    32941 gcaagtttgc gaaaactttt ggagtcagcc gtcagatcat tgtaggcgat attgctttac
    33001 tacgagctgc aggtaaaagt atcgtggcaa cagcaagagg ttatatcctc gaaacagaag
    33061 aaaccaatac tgggcacatc agtaaaatcg ctgtccagca tggaaaagat caaacagaag
    33121 aagaactgag actaatcgtt gaaaatggtg gtgaaatcat tgatgtaatt gtggaacatc
    33181 ccctttacgg tgagttagca ggaactttgc acatcaaaac gccacaagat atcgattcct
    33241 ttatgagacg ttatcaaaaa agtaatgcta ctttgttatc cgaattaaca ggcggcatcc
    33301 atctgcatac gatccgctac ccagaaaagc atatgctgaa aaacatcaaa caaaaactag
    33361 ctgaagcagg aatcctctac gaaggataac atcgtagctg ctacttttta tcctagaaac
    33421 tattctatca tctctgagat aaacacccat atggaagaaa taagaaaaaa taagaaaaga
    33481 tgcgtatcca atacacagaa ttttttggaa ttgcgatttc tagatgtcta tctaaatttc
    33541 gattagtgga gaaacataaa aaagggaagt gcagcttttg agcactcccc ttttttataa
    33601 agctttcttg tagcatagta tcgaaggaac ctgccaattc tctactcgat caccaatacg
    33661 ccttctttta gtgcaaatgg attttgatcg ttgatgtggt catagaacat cacaccgttg
    33721 atatggtcga tttcatgttg aacgacgatc gattcataat tcttcaaacg aatcttgtgt
    33781 ttttcaccat tcatatcata ataagacaca gtgattttgg catggcgaac aacatatcct
    33841 ggtacttctc gatcaacaga taaacagcct tcaccttccc ctaaacaagc atcttgaaca
    33901 gaatggctca aaattttagg attatacata acagtactta gagaaggtcc atctgcctct
    33961 gggtcaggac ttggtacgtg aacagcaatg attcttttag aaatatctaa ttgaggagca
    34021 gctaagccaa caccgcctcg taagtgtagt tcttcagctt tgattggatc ttggctgttt
    34081 tttaagaact caagcatctc ttttcctaaa gagatatctt cttcgcttaa tggaagagaa
    34141 acttcttttg caacttcgcg taaggtcgga tttccttcac gaatgatatc atccattgta
    34201 atcatggaat atgtacccct ttcttaaaag actgtgtttc aagtctattt tcgcattata
    34261 gtttaccaca aaaatggaaa agtagtagta agattgtttg aaaactacct tttcttaatg
    34321 attaactact tgattttcaa cccggtttca ggtaaagtat agctgtgtgc tctaggcaca
    34381 tgacctgcca cttggttaag cgaatcacca acgttttact cagtcgcagg ggaataatcg
    34441 aaagaggtga aagaaatggc aatttcaaaa gaacgcaaga atgaaatcat caacgaatac
    34501 gctcgccatg aaggggatac tggttctcca gaagtacaaa tcgctgtatt aactgaagaa
    34561 atcaaccact tgaatgaaca tgcacgcgtg cacaaaaaag accatcattc ataccgcggc
    34621 ctaatgaaaa aagttggtca ccgccgtaac ttgttagctt acttacgtaa aactgacgtt
    34681 caacgttacc gcgaattgat caaacgttta ggcttgcgtc gttaatttta gtcaaaaaga
    34741 gtgggattca catgaatctc acttttttta ctcgtatatc aattaagtaa aattccgtga
    34801 tcaagctaga tggactccta ctaaccaaat gaaagctgct caaaagacgg ctttcggcag
    34861 ttttcatttg tttagtagcg tcttgctttc acgagaagga aaaggagaag ttatgtcaga
    34921 aaaacaagta ttcaaaacga catggggcgg ccgtccctta caaatcgaag tcggtcagct
    34981 agcaaaacaa gcaaacggag ccgttttagt ccgttatgga gatacagtag tcttaagtgc
    35041 agctgtggcc tctaaagaag caaaagatac agactttttc ccattaacga tcaattatga
    35101 agaaaaaatg tatgcagcag ggaaaatccc tggaggtttc atcaaacgag aaggccgtcc
    35161 aagtacggaa gcaacattga ctgcccgttt gatcgaccgt ccaattcgcc cgatgtttgc
    35221 agaaggcttc cgtaatgaag tccaagtaac gaacatcgtg atgagtgtag aaacagactg
    35281 ctcgccagct atggcagcaa tgctaggttc ttctttagca ctatcaatct cagatatccc
    35341 atttgacggc ccaatcgcag gcgtagaagt tggtcgtgta aatggcgagt acgtcttgaa
    35401 tccaacagta gaacaagcag aacaaacaga tatcgaattg actgttgccg gaacgaaaca
    35461 agccatcaat atggttgaaa gcggcgcgaa agaagtttca gaagaagaca tgcttggtgc
    35521 attgctgttt ggttttgacg ctatcaaaga gttagttgct ttccaagaag aaatcgttca
    35581 agcagtaggg aaagaaaaaa tggaagtgac tttactgcaa gttgatgaag tattgaaaaa
    35641 agaaatcttt gatgcttctt acgctacaat gaaagcagca gtaatgactg aagaaaaact
    35701 tgctcgcgaa gataatatcg agcaggtgaa gatcgatatc cgcgaagctt atgctgagaa
    35761 atttgcaggt catgaagatg aagatcactt gttgaaagaa gttaaacaaa tcacagaaga
    35821 tcttgaaaaa gatgtcgtac gtgaattgat caccatcgat aagatccgtc ctgacgggcg
    35881 taaattggat gaaatccgtc cattgtcatc agaggtcagc ttattgccac gtgtacatgg
    35941 atcaggtttg ttcacacgag gacaaacaca agctttatca gcttgtacac tcgcacctct
    36001 aggagagcac caaatcattg atggtttagg ggtggaagtc agcaaacgat tcatccacca
    36061 ctacaatttc cctcaatttt ctgttggttc tacaggacgt gcaggctctc cagggcgtcg
    36121 ggaaatcgga catggcgcat taggtgaacg tgcgttagca caagtaatcc cttcagaaga
    36181 agaatttcct tatacgatcc gtttagtagc agaagtattg gaatcaaatg gttcttcttc
    36241 ccaagcaagt atctgtgctg gcacattagc attgatggat gcaggtgtac caatcaaagc
    36301 gccagtcgcc ggtattgcga tgggacttgt ttctgatggt gaaaactata cgatcctaac
    36361 tgatatccaa gggctggaag atcatcttgg cgatatggac tttaaagttg ccggtacaaa
    36421 agatggtatc actgctttac agatggatat caagattcaa ggaatcacag aacaaatcct
    36481 aactgaagca ttaactcaag caaaacaagc acgtatggaa atcttagaag aattgacttc
    36541 aacgattgct gctccacgtg aagaattaag ccaatatgca ccaaaaatcg aaatgatcca
    36601 gatcgaacct gctaaaatca aagatgttat cggtaaaggc ggcgatacga tcaacggaat
    36661 catcgatgaa actggtgtga aaatcgatat cgatcaagat ggaaaagtaa gtattgcttc
    36721 agctgatgca gaaatgatca aaaaagctat caagatcatc gaagatttga ccaaagaagt
    36781 caaagttggt gaagtttatc tagggaaagt tgtccgtatc gaaaaattcg gtgcattcgt
    36841 caacttgatc aaaggaaaag atggacttgt ccatatttcc caattagcaa acgatcgtgt
    36901 caataaagtc gaagatgttg ttaaactagg tgatgagatc cttgtgaaag taactgaaat
    36961 cgataaacaa ggacgcgtaa atctgtcaag aaaagcaatg ttgaacgaag atggttcaac
    37021 gaaaaatgaa gaaaaataat ttaattcata ggaataatgt acttgtgatc gtgaaggtga
    37081 cagggggaca ataattattc gaaaaaaggg gactgtgaca aaagccgtgt cacagtcctt
    37141 ttttttcgaa taaacggtgt tcaaaagctt gcatctgcgg taataagtcc agccaagtta
    37201 aaaacattag gagctgtttt tatttggctg ttcttattac ttgatgcaga acagcttttt
    37261 tacaacctct ccaataaacg atgttcaatc agctgtccct cgatattagc tcaaaaaacg
    37321 gcaaaatatg aggaactatt tttccgtttg tttctctaat actcaggtca aaccgctgat
    37381 ttcacaacct ctgattcacg gtgttccaga agcagcttct acggaaataa gctgaacact
    37441 tttgttacaa cttctttttt tctggagttt cttggcgcat atttttggag tccttctgaa
    37501 taaagcgtaa aataaaagtg agaaatatgg ataaaggagg gaaaggagat gctgaatttt
    37561 ttatggtcgt tatttatcgg cggtatatta ggtttgatag ctggcgcaat tttaggaaag
    37621 gaagtcccgg gaggcgtatc tggaaatatc attatcggtt ttttaggtag ttggctcggc
    37681 gaatttttac taggaccact gggaccggaa ctcggcggtt tttatttgat accagcatta
    37741 ttgggtgcaa ttttttgcct tgctctttat tcatttgtgg caagacgtat cagagaacgt
    37801 ccaaagaaag aataatttta ttcaaataag ttgacacttc agtgtgttac ttggataatt
    37861 agaagcaatt taggaaagcg ggtgtcatgg ataagaatat catctatcat tcaagcttac
    37921 ctaaaggata tccatataac aagaaatgag ggaaaaacgt ggcaacaacg cacaatacca
    37981 aaaagatatc aatgactggc ctgcttgtag cgatgggagt cgtctacgga gacatcggga
    38041 caagtccgct ttacgttatg aaatcgattg ttgaagataa tggcggattg tcaggaatca
    38101 atcctgattt tgttatcgga tcagtctcat tgattttttg gacattgact ctactgacaa
    38161 caatcaaata tgtcgtgatt gcattgaatg cagataacca tggtgaaggt ggaattttct
    38221 cactctacac acttgtccga aaaggtggga aatatttgat cattcctgca atgatcggag
    38281 gagctgcctt actagcagat ggggtattga cccctgctgt gacggtaacg actgcgattg
    38341 aaggcttgcg aggaattccg cagttttttg accggttcgg taataaccag aacgtgatcg
    38401 tcattatcac tttagttatt atttttgttt tattcatggt ccaacgcttt ggtacagata
    38461 ttgtcggaaa agcattcgga ccaatcatgt tcgcttggtt tactttttta ggcgtaatgg
    38521 gattaatgaa cttcagtcaa gactggacag ttatccgtgc actgaatcct tattatgcta
    38581 tccagctgct ttttagtgca gataacaaat taggcttgtt cattcttgga aatattttcc
    38641 tggctacgac aggtgcagag gcgctttatt ccgatcttgg acatgtcggc aaacgaaata
    38701 ttcgtctgag ctggccttac gtcaaaattt gtttggttat caattatttt gggcaggctg
    38761 cgtggttgtt gaatgtttat caaaaccctg aagcacagca aatcaagaac ctgaatccat
    38821 tcttccaaat gatgcctcaa agttggatcg tgatcggcgt tgcttttgca acagtcgctg
    38881 cagtcattgc ttcgcaagca ttgattacag gttcgtttac gctagtttca gaagcaatca
    38941 agttgaaact gctgcctcgg ttaaagatca tgtatcctgg gaacagtatc gggcaaatgt
    39001 atatcccagc agtcaatctg attttatgga tcgcctgttc tctagtggtt gtcacattcc
    39061 gaacgtctca tcatatggaa gctgcttacg gtctatcgat cactgtgacg atgttgatga
    39121 caacagcact gctttacttt tatcttatcc aaaatgggca ttctcgttgg cttgcatatt
    39181 tagttacctt tttcttcggt gcgatagaag tcgttttctt tatctcaagt gttgtcaaat
    39241 tcttccgagg cggttttgta gccgttctga ttggtttgat catcttagtc gtgatgttta
    39301 tttgggaaca agggaacatt attcgcgaat cgtcaaccga ggatgtgtct ttgagagatt
    39361 atatcccgca gcttagagaa ttacgtgatg atacttcttt accaatctat cagacaaatg
    39421 tggtcttctt ggttcctgat atggctgaag ataaagttgg ccgacagttc atctattcga
    39481 ttttagataa acggcctaaa agagcacgag tctattggtt tgttcatgtg gaagtgacgg
    39541 atgagccata tacgaaagaa tatcaagtag atatgatgga cactgatttt attgtccaag
    39601 tgaatctgtt cttaggtttc agggtccaac aggaaatcaa cgtatatgtt cgccaagtca
    39661 tccatgattt gatgaaacaa ggccgtttgc caaaacagcc acaaagatac tctctgacac
    39721 cagggcgaga agttggcgat ttccaatttg tcctgattga tgaagtagtt tccaatgtga
    39781 caactctagg aaaatggcaa cgtcaaatca tgcaggcaaa actggcaatc aaaaaaatcg
    39841 ctacctcgcc agaaacatgg tttgggctag agtacagcga agtaaaacat gaaactgttc
    39901 cgcttatcat tggaaatact cggaaaacat ggttgaaaga gcggttgtga aagtagcgct
    39961 ttgacctgaa ctaatgtcga atgtcagcta cttgaacacc gtttatcaga ggtcatgaaa
    40021 aagctgttta gcaacaagta acgcaatgat tttgaagtga taaggagatg tggctgaaaa
    40081 atgccgcatc tcctttttta ttcctaaata tgaatacgca ttattttcaa tctttttgta
    40141 gaaaaagggt acaatagaaa aagagaaagg cgggagaata tggttatcaa tttcattatt
    40201 ttatttttga tgattgcaat tacttcattt tttgtagcaa gtgaatttgc tttagtgaaa
    40261 atcagacgat caagattgga acaactagaa aaggaaaatg taaagaacgc caagctagct
    40321 cttcatgtta cacaccattt agataactat ttatcagcta gtcaattagg aattacgcta
    40381 accggtttga tcatcgggtg ggtcggtgaa ggatctgtcg cggctttact agaaccagtg
    40441 atcggtaaac taccttttag ttcggctatt agcagtacaa tctctgtggc gctgggattt
    40501 attttagtta cgtatatcga tgtggttatt ggcgaactgc ttcctaagag ctatagtatc
    40561 gtcaatacgg agaaggtcgt cttgttcgta gttaaaccac ttcattattt ttataaagta
    40621 atgtttcctt ttatttgggt attgaatcat tcagctgcag ggctagggaa gctattaggt
    40681 gtgcgtctcg tatctgaagg agaagagaca ttgtctcaag aagagctgac attagtcgca
    40741 ttgaattctt atgaaaaagg agagttgacc aaagaagaat attattattt ggaaaacgtg
    40801 tttgaatttg atgatacctt ggcaaaagat atacaagtag accgtacgtc catgcaagta
    40861 ttcgaagctt cagaaaccgt caaagaagcg ataaaagctt cgattgaaca aggacatact
    40921 cgctatccgg ttatcttaga atcaaaagac aatgttttag gttatgtcac cttgccagat
    40981 ttgatcaagc aatcttataa agatgaccag ctgacagtag aacagttgat tgaagagccg
    41041 atcgtaacaa ctgaaacgat ccctataaaa aaattattga cgatcatgcg aaaaaagggt
    41101 aagcatattg ctatcttaaa agatgaatac ggagggacaa gcggcttagt gacgattgaa
    41161 gatattttag aagaaatcgt cggggaaatc cgagatgaga cagatttgga cgaagcattg
    41221 atagcggaac agtcagatgg ctcttatatc atctccggca aactaacatt agatgatttc
    41281 cagcgttatt ttcatgtaga gattccagag tttgaagaaa cgaattttac tacacttgcg
    41341 ggctttgctt ctagccgata taaagaaata aaagcaggaa caatcattga gattgcatca
    41401 ttccgtttca cggtattaga ataccagcat gcacatatcg attatttcaa agtagagtcc
    41461 acggaaagaa aaacagaata aaagaaaaaa ggctgtgaca ttttttgtca cagccttttt
    41521 gttatctaaa aaacctgttt tcggtaaacg aagaagatat tttcaataaa agttttcatt
    41581 tttagccgca acgatttctt ttaatcaagg ctatcttgct tctgcatgtt ctaatgtgaa
    41641 atgttcctct tcacccatgt gtttcaattg tttcatcgaa cgcatataag agtaaagcaa
    41701 cacggcaaca gaaccgatcc aagctaaagg agaagctgct gctgctcctg ggtatcccca
    41761 cattcctgta agaacgatag ctgcaaaaga acgcataagc aattccatca taccggcgat
    41821 cgtagggatc ttgctttgtc caagcccttg aagtgtgtag cgtaaaatga acaggactgc
    41881 taagatccag taaagagacc cattgatatt gaagtaggtt tgtgctaatt caaatacacg
    41941 tgtttctgat gggctaacaa acaattgaac aaagaaatga ccgaagaaaa tgacgatagc
    42001 acctgctaag agactaaatc cgacactcat cagtaaagtt tgacgtaccc cttgcaagat
    42061 tcggccatat tctttcgcac catagttttg tgcagtgaaa gttgccattg caataccaaa
    42121 tgacatcatc ggctgatttg caaattggtc gatacgacca gcagcggctt gagcagctac
    42181 gacatctgtc cctagactat ttagtgctgc ttgtaacacg atagcaccaa cagcgatgat
    42241 cgaagattga aacgccattg gcaaagcagc atttaggtgt acagcaaact cttctttttc
    42301 aaatttgaaa tcttttttgc gtaactgtaa caaaggcatt tttttcttga tatacactag
    42361 acacagtacg ctggaagaaa cttgtgcgat tactgtagca atagctgcac cttccacacc
    42421 catgccaaaa tagataataa agatcaaatc aagtacgacg ttgatgatga ccgcaatgat
    42481 cagaaagaat agtggtgtgc ggctgtcacc aagtgcgcga atgacattag aaagcaaatt
    42541 gaaactgact gatgcaaaga tccctaataa aataattgaa ataaatgttt gtgcttgatc
    42601 aagtatttca ggaggagttt gcatcaattg aagcatcgga cgaataaaca ggagacttag
    42661 gacagttaaa atcacagtga cgacaagact aatgacaaca ctagcagcaa aacttttctt
    42721 cagaccccga taatcttttg caccgtaacg ttgtgcggta ataatggcta aaccagctgt
    42781 taaaccttgg gcaaagccaa tgatcaaaaa tgtcaggctt ccagttgcac caaccgctgc
    42841 cagcgcattt ttacctagcg tctgacccac gatgatcata tcgatcatat tgtagaactg
    42901 ctgaaaaaca tttccgatca gtaaaggaac agtgaacatc aaaatcagtt tagcggggct
    42961 tccttttgtt aaatcgcgca tcttttaaac tccttatatc tttaaaattt agttagtagt
    43021 atatagcgtt tatcctgaga ataccaatta gaaatcaatg ataatttatg aaactccata
    43081 aaaagaagcc aagaattgag tcaaagaatc aacaacttgg cctggtctgt aaaataaggg
    43141 acatccacgt ttatctatca ccttaatatt gtctaatctc acttggtaaa aggatcgttt
    43201 ctctcgtacc gtcatcatag gcgtatactt gagcaggaaa ttggttcgaa taggcaaacg
    43261 gcaaatcaat atccatttcc acattgcttc ctgcttgaga aaaatgcatc gtcacttttc
    43321 cacgattttc gatcaaatca aaggttaata tattttctaa tggaatgact cccttaagat
    43381 ctaggtcaat aataaaccat atactgtcaa tcaattcatc tggcaaactt gatacgacac
    43441 caactgaagc gtaacggttg cggctgctat caaaactttc aaacatctgt ttaacctcct
    43501 tcgaatcaac gaatgtttta tttttataat taatcctagg ataaactaga aatacttact
    43561 tcaacgaggg ttcattctaa taaagaatca ggaaaaatgc aacaataatt gaatactttt
    43621 tttcaaagta taaattttta taaaggtacg ataaaagtat gcgaggaaaa gcttggtaaa
    43681 ttaggatttt tcgtttgtaa agccatttac aaaagtggaa gccccatatg aaaataaacg
    43741 attagaaata aaaaaggcgt gttaaaagct gctgacccgc cgcttctaac accacctgat
    43801 tgtttcagta ttattcttct gttgaaccga tcacttctgg ttcgccacct tcacctgtgc
    43861 tttcttcttc ttcactttga gcgactacag cagcgatttg ctcttcactg tcggtcacga
    43921 atttgtaatc gtcgtttttt ggaagatcgg ctactgtaat agaatcacct aattccaagt
    43981 ttgtcacgtc aacttcaatt cgttcaggca atttgtctgg tgtagcagat acgactactg
    44041 tatacaagtt ttgagctaac actccaccag atttagcacc cactgattcg ccaacaagaa
    44101 cgacttctgt ttcgacttct gtttcttccg acatgtttac agatagaaat tctgcatgtt
    44161 cgaaatgatt ggtaaaagtg ttcgtttgga tcttgtgaac caatgtattg actttttgtc
    44221 catcgatgtc catcgtgatg actgtgttcg ctccatgttc acgcaacaat ttgcttagtg
    44281 tcaaaccatc gaatgagata ggggtacttt ctactttata cccataaaca acagctggaa
    44341 ctttcccttc gtgacgaagt ttgtttctta atgaacgggg acgtacttct cttttactta
    44401 cttctaatga tactgacata accaaaattc ctccttaaaa tgtgactgtc tgctttggta
    44461 acggacagat tttatttaag tgaattctaa aggctaatct cgtgcacatc atcgaaggca
    44521 gggcgcttaa aaaagcgcaa aaggagagat gcgcgcatct ccccttagaa agtcaggatt
    44581 caacttttct ccaccaggtc attacgctgt gtatgagaaa tccaattcac tttactattt
    44641 aaccgtaacg tgaactgtta attaagtcaa ggaataacta tgatgatgta tttgcaagca
    44701 agaatattca aggtataatt aattagtagt aataagaaag gaaacagcat gcgtttagat
    44761 aaattaatag aagaaaaatt agaaacgaca cgcaaggaaa tgaagcggct gtttgctttg
    44821 aagcaagtga tgatagatgg cacagtcgaa tttcgacaaa atcgaaatgt agatagttta
    44881 ctgcatcaaa tcgaagtagc aggtgaacac ttagaaacaa atgagtccta ttatcttctc
    44941 aataaacctg aaaaagctgt tacagctgta tcagatccag aaaagacgac agtaatcgat
    45001 ctgattgcaa gtgccgatcg tttccaacca ttatatccag tgggaagact agatagagat
    45061 acaacaggac tattgctgat cacttcgaat ggacaattgg gatatgaaat gctacttcca
    45121 gaaaaaaagg tttcaaagac ttatcaagta gttgtgaacg aaagggtgac accggcagac
    45181 gtaacagcat ttgaaaatgg aatcgtattc gctgatgggt atcaatgtaa gccggctgat
    45241 ttaaaaatac tcaaagcaga caagcaccaa agtacggttt tattgacgat ccaagaagga
    45301 aaattccatc aagtcaaaaa gatgttctta gcccaaggaa agaaagtgac cgcattgaaa
    45361 cggctaagca tgggaccatt ggaattagat gaacagctag gagtaggcga gtatcgatcg
    45421 ttgacattag cagagatgaa aaagttaagt atttatttta aataggaggt caaaaagatg
    45481 aaatataaaa ctttattgtt tgatgtagat gatacattgt tagattttca attaactgaa
    45541 gccaatgctt taagtgcttt gtttgcagaa caaggaatca cattgacacc agaaatcgaa
    45601 accagctata aggctttgaa tcatcattta tggcaggagt ttgaaaaagg cagactatca
    45661 cgagaagaaa taataggcag tcgttttggg ttacttttcc agcaatttgg gaaaacagta
    45721 gatagtgatc aaatggaaaa acgctaccgc cactacttga accaaggtca tgatcttgtt
    45781 ccaggaagtc tagaaatcct gaaaaaggtc catccgcatg ctgatcttta tattgtgacg
    45841 aacggtgtgt cgcacacgca acatcggcga ttaacagatg caggaatgct tgattacttc
    45901 aaggatatct ttgtctcaga agctgtcgga gcgcaaaagc cgatgaaaga attttttgat
    45961 catgtctttt cccacattcc taattttgat aaaaagcaaa ctgtgatcat tggtgattct
    46021 ttgacttctg atatcaaagg aggaaatatg gcagggatcg atacgatttg gttcaataga
    46081 aataaattac cagaaatcga agaaatccag cctacttatc gaatcgattc attagaggaa
    46141 ctttattcat tattggaaat tgaaaaggat taattttcag aaaaatatag cattttcgtg
    46201 ttttttcgct ataatagatt agaatgaaaa aacggatacg aaagaggttt tagtcaatga
    46261 caaaaagcaa gccaattatc attggtgtga cgggtggatc cggaagcgga aagacaagtg
    46321 tcagccgtgc gattttcaac aacttcccgg accattccat catgatgctt gaacaagatt
    46381 cttattacaa ggatcagagc catttgagtt ttgaagaacg attgaacaca aattacgatc
    46441 acccatttgc ctttgataat gatctgctga ttcagcatgt aggcgactta ctgaactata
    46501 aagccattga aaaacccgtg tatgactatg tagcgcatac aagaagtcag gcaacgatca
    46561 ttcaggaacc aaaagaagtg atcattctag aaggaatcct gattttagaa gacgaacgtc
    46621 tgcgtgactt gatggatatc aaagtatacg tagatacaga tgatgacatt cgtatcattc
    46681 gtcgtatcaa acgggatatg gaagaacggg gacgcacatt ggactccgtg atcgaacaat
    46741 atttgacagt tgttaaaccg atgtatcatc aatttatcga accaacgaag cgctatgcgg
    46801 atatcattgt tccagaaggc ggagaaaacc acgttgctat tgatttgatt acaacaaaag
    46861 tagctagttt cttaaaccat aaatagtgcg cagcgaaaca gttgaacgga ctgcgaccgt
    46921 ttgaaaagca aggattctgc tttttgaaca gtcaccagcc aactgttttt ttatttataa
    46981 caagaggttg tgaaaacagc atcttgacct aagtattaaa aagaagaaac ggaaaatagc
    47041 tgtttatatt ttaccgtttt ttgagctaat accgaaggtc agctgattga gcaccgtgaa
    47101 gtagaggttg taaaaaagct gtctttgcat caagcgataa gagcatccaa ataaaacagc
    47161 tcctcacgtt tttcgttggg ttgggcttat tatcgtagat gcaagctttt gaacaccgtt
    47221 taacgagagg ttgtaacaga agtggtcaga tgcaataaat aagaagaaac ttacgaaaat
    47281 tatttctcga atttgtgtga gatttcgact tatttttttg aagaagctct tttgtcgcac
    47341 ccccta
//
LOCUS       DACCXO010000010        47214 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.010, whole genome shotgun sequence.
ACCESSION   DACCXO010000010 DACCXO010000000
VERSION     DACCXO010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.010"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..760
                     /locus_tag="IVY70_000511"
     CDS             <1..760
                     /locus_tag="IVY70_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957671.1"
                     /translation="YVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSASAGEY
                     ESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISELLYFGH
                     MKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKINVKKS
                     FFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIWKLKGT
                     RYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(841..1410)
                     /gene="thiT"
                     /locus_tag="IVY70_000512"
     CDS             complement(841..1410)
                     /gene="thiT"
                     /locus_tag="IVY70_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP7957672.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(1476..1578)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1584..2555)
                     /locus_tag="IVY70_000513"
     CDS             complement(1584..2555)
                     /locus_tag="IVY70_000513"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP7957673.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(2649..4022)
                     /gene="glmU"
                     /locus_tag="IVY70_000514"
     CDS             complement(2649..4022)
                     /gene="glmU"
                     /locus_tag="IVY70_000514"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP7957674.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(4525..5343)
                     /gene="purR"
                     /locus_tag="IVY70_000515"
     CDS             complement(4525..5343)
                     /gene="purR"
                     /locus_tag="IVY70_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP7957675.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(5510..6334)
                     /locus_tag="IVY70_000516"
     CDS             complement(5510..6334)
                     /locus_tag="IVY70_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP7957676.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(6307..6987)
                     /locus_tag="IVY70_000517"
     CDS             complement(6307..6987)
                     /locus_tag="IVY70_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP7957677.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(7010..7948)
                     /locus_tag="IVY70_000518"
     CDS             complement(7010..7948)
                     /locus_tag="IVY70_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP7957678.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(8193..9044)
                     /locus_tag="IVY70_000519"
     CDS             complement(8193..9044)
                     /locus_tag="IVY70_000519"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP7957679.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(9198..11771)
                     /gene="mprF"
                     /locus_tag="IVY70_000520"
     CDS             complement(9198..11771)
                     /gene="mprF"
                     /locus_tag="IVY70_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP7957680.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            11912..12718
                     /gene="proC"
                     /locus_tag="IVY70_000521"
     CDS             11912..12718
                     /gene="proC"
                     /locus_tag="IVY70_000521"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP7957681.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(12797..14353)
                     /gene="rny"
                     /locus_tag="IVY70_000522"
     CDS             complement(12797..14353)
                     /gene="rny"
                     /locus_tag="IVY70_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP7957682.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(14764..15813)
                     /gene="recA"
                     /locus_tag="IVY70_000523"
     CDS             complement(14764..15813)
                     /gene="recA"
                     /locus_tag="IVY70_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP7957683.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(15922..17163)
                     /locus_tag="IVY70_000524"
     CDS             complement(15922..17163)
                     /locus_tag="IVY70_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP7957684.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(17403..17981)
                     /gene="pgsA"
                     /locus_tag="IVY70_000525"
     CDS             complement(17403..17981)
                     /gene="pgsA"
                     /locus_tag="IVY70_000525"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP7957685.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(18190..19074)
                     /locus_tag="IVY70_000526"
     CDS             complement(18190..19074)
                     /locus_tag="IVY70_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7957686.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(19852..21138)
                     /locus_tag="IVY70_000527"
     CDS             complement(19852..21138)
                     /locus_tag="IVY70_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP7957687.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(21131..22399)
                     /locus_tag="IVY70_000528"
     CDS             complement(21131..22399)
                     /locus_tag="IVY70_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP7957688.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            22651..23112
                     /gene="mscL"
                     /locus_tag="IVY70_000529"
     CDS             22651..23112
                     /gene="mscL"
                     /locus_tag="IVY70_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP7957689.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(23171..23617)
                     /locus_tag="IVY70_000530"
     CDS             complement(23171..23617)
                     /locus_tag="IVY70_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7957690.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(23710..23955)
                     /locus_tag="IVY70_000531"
     CDS             complement(23710..23955)
                     /locus_tag="IVY70_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957691.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(24070..24648)
                     /locus_tag="IVY70_000532"
     CDS             complement(24070..24648)
                     /locus_tag="IVY70_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7957692.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(24725..25255)
                     /locus_tag="IVY70_000533"
     CDS             complement(24725..25255)
                     /locus_tag="IVY70_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP7957693.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(25259..26119)
                     /gene="rlmB"
                     /locus_tag="IVY70_000534"
     CDS             complement(25259..26119)
                     /gene="rlmB"
                     /locus_tag="IVY70_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP7957694.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(26112..26507)
                     /locus_tag="IVY70_000535"
     CDS             complement(26112..26507)
                     /locus_tag="IVY70_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP7957695.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(26504..27916)
                     /locus_tag="IVY70_000536"
     CDS             complement(26504..27916)
                     /locus_tag="IVY70_000536"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP7957696.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(27913..28449)
                     /gene="cysE"
                     /locus_tag="IVY70_000537"
     CDS             complement(27913..28449)
                     /gene="cysE"
                     /locus_tag="IVY70_000537"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP7957697.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(28771..30231)
                     /locus_tag="IVY70_000538"
     CDS             complement(28771..30231)
                     /locus_tag="IVY70_000538"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP7957698.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(30401..31558)
                     /locus_tag="IVY70_000539"
     CDS             complement(30401..31558)
                     /locus_tag="IVY70_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP7957699.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(31592..32962)
                     /gene="radA"
                     /locus_tag="IVY70_000540"
     CDS             complement(31592..32962)
                     /gene="radA"
                     /locus_tag="IVY70_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP7957700.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(33025..33552)
                     /locus_tag="IVY70_000541"
     CDS             complement(33025..33552)
                     /locus_tag="IVY70_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP7957701.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            33782..34588
                     /gene="proB"
                     /locus_tag="IVY70_000542"
     CDS             33782..34588
                     /gene="proB"
                     /locus_tag="IVY70_000542"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP7957702.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            34585..35832
                     /locus_tag="IVY70_000543"
     CDS             34585..35832
                     /locus_tag="IVY70_000543"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP7957703.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            35908..36252
                     /locus_tag="IVY70_000544"
     CDS             35908..36252
                     /locus_tag="IVY70_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957704.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(36369..36695)
                     /locus_tag="IVY70_000545"
     CDS             complement(36369..36695)
                     /locus_tag="IVY70_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957705.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(37310..38512)
                     /locus_tag="IVY70_000546"
     CDS             complement(37310..38512)
                     /locus_tag="IVY70_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7957706.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            38629..39144
                     /locus_tag="IVY70_000547"
     CDS             38629..39144
                     /locus_tag="IVY70_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7957707.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(39343..39789)
                     /locus_tag="IVY70_000548"
     CDS             complement(39343..39789)
                     /locus_tag="IVY70_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP7957708.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(39836..41314)
                     /gene="msr"
                     /locus_tag="IVY70_000549"
     CDS             complement(39836..41314)
                     /gene="msr"
                     /locus_tag="IVY70_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP7957709.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(41668..42297)
                     /locus_tag="IVY70_000550"
     CDS             complement(41668..42297)
                     /locus_tag="IVY70_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP7957710.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(42281..42745)
                     /locus_tag="IVY70_000551"
     CDS             complement(42281..42745)
                     /locus_tag="IVY70_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7957711.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(43232..44500)
                     /locus_tag="IVY70_000552"
     CDS             complement(43232..44500)
                     /locus_tag="IVY70_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP7957712.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(44731..45468)
                     /locus_tag="IVY70_000553"
     CDS             complement(44731..45468)
                     /locus_tag="IVY70_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7957713.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(45535..46104)
                     /locus_tag="IVY70_000554"
     CDS             complement(45535..46104)
                     /locus_tag="IVY70_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP7957714.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            46232..47104
                     /locus_tag="IVY70_000555"
     CDS             46232..47104
                     /locus_tag="IVY70_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7957715.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
BASE COUNT        13373 a        10162 c         8392 g        15287 t
ORIGIN      
        1 ttatgtacat attgatacca caagtaatgc tgttttaaca aaagggctga ctgctactga
       61 ctttgcaaac agtatcgtgc attttcctca aaacttgctc ttgcttgatc catctgcttc
      121 tgctggagaa tatgaatccc atacagggct gaaagtaatc cgtggtacag aaaatattca
      181 gcgtttcttt tcctcttctc gtaacaggcg tggattcgat gaattgaagt ggatcgactt
      241 tactgattta acgatgctga aagaattgac acctttagaa atttcagaat tattatattt
      301 tggtcatatg aaaacacatc ttcattcgcc gtttttttat aaactacaga ataattttgt
      361 atacttcgat cttaatgatc agctgaatcg gatttattat cgctatttag atgaatttta
      421 taggattttt gctagtaaat taagttcgat cttgtcagat aagatcaatg taaaaaaatc
      481 ttttttcaaa aaagaaacac ctgttgaaaa aatcagtgtg gaattattga aagaaatgcg
      541 taatttgatg caggaaggga tcattttttc ttttgatcaa gttggtttga tcgatggtaa
      601 atatcgtatc ccaatctatg tgattgaaga ttatatttgg aaattaaaag gcacccggta
      661 tcgagaagag gaaatcatcg ctactttaat ctactctgtc accgatcgac actggcaatt
      721 ggtagaagat gccgatcttt cttatctgcc attgaagtag atctatttct tatcaaaaaa
      781 taaaaaggaa acaagaaact aggacatgat tctgtctcaa gtttcttgct tcttttttat
      841 ttacagctcc tgtcgtttat ctggcacaaa aatcgaagga gtcgtttgtt ggatccctaa
      901 taaaacgatg atcgttacaa tagctgtact caacccgctg gcaccattca ttacgaaaga
      961 aaagagccaa ggattcatcc cccatagtgc atacgctccc cagaaaacca ctcctgcaat
     1021 aaaatgccaa aaatatctgg aaagtgcgcc aatcaatgca ccaaacacga tgctttttct
     1081 aatttgtttc gtattatcgt ctacatagcc tttttggaca tttgaagcca tgagccctgc
     1141 tgcgcctgca aaagtatagg caatcgggta ctcaatcaac acttgaacga cacttaaata
     1201 ataaacttgt cccaacggaa aatgtaggat gccccaaatg aaacctgcca gcatccctgc
     1261 ctttgtccct cttcttaacg caaaaagagt caatggaatc tgccctaatg aaatagaaaa
     1321 actgctgccg atctggatcg gaatcaacga caacaccatc gctaacgctg caacaatcgt
     1381 tccttctacc caaactctat tttttcccat aaaaaaactt cctcctttga ataaaaaagt
     1441 cacaaaggag gaagcagctt tcgaaaatag tcaaacagct gtttattcac aattcctacg
     1501 ctcgcataac cgatacaggt acgaagggtt tagaaataga ttcaatctca gcccaattgg
     1561 actccccttt gtgagtttat ttattattta ttttttgttt caaacaatgg actaactggt
     1621 ttgttttcat ggatacgttt gattgcatct cccattagtc cgccaacact tacttcatcg
     1681 attttgtcga ttttacgatc ttctggaaga tagatcgagt ctgtaacaac tagacgctcg
     1741 atagctgaat cttctattct ttgtaaagca gggccggata atactggatg tgtacatgaa
     1801 gcatacacac tagttgcacc agcttctttc aaagcattgg cagccaatgt gatcgtacct
     1861 gctgtatcga tcatatcatc gatcagtaca caagttttgc ctttgacttg tccaatgata
     1921 ttcattactt ctgcaacatt tgcttttgga cggcgtttgt ctatgatcgc aatcggagat
     1981 ttcaaaaatt ccgctaattt acgtgcccgt gtgacaccac catggtctgg tgaaacaacg
     2041 acaacatcat cgcctttgat tcctttttct aagaaataat tggcaataag tggcgcaccc
     2101 atcaaatgat cgactggaat atcaaagaaa ccttggattt ggactgcatg aaggtccaaa
     2161 gtcagcattc ttgttgcacc agctttttga atcatgtttg ctactaattt cgctgtgatt
     2221 ggttcccgtg cacgtgcttt acggtcttga cgcgcatagc cataataagg catgactacg
     2281 ttgattgttt ttgcacttgc tcgtttcagt gcgtcaatca tgatcagcaa ctccatcaag
     2341 ttatcattga ctgggctgct cgttgattga ataacataga catgcgatcc acgaatactt
     2401 tcttcaatgt ttacttgaat ttctccatca ctaaattgag taacagatga ttttcctaac
     2461 tcgacgccga ctgctgctgc aattttctct gctaatgggc gattggaatt taacgcaaaa
     2521 atttttaatc ttgggtcaaa gtaatgtttt gacatgggga cctccacttt ctttttcaca
     2581 actctagaat tctacctaat aaatagtagt catatttttt aaataaatca agtgattttg
     2641 caaaagtctt agtttagata agggagtttt tttgcatatc cttctttatt gacttgtctt
     2701 gctctcgcaa ttgccagcgc atattctgga atattgtctg taattgttga cccggcagca
     2761 atcgaactgt ttttagctac ctcgacaggt ccgatgatat tcgtgctaga accaataaag
     2821 ctgtggtcgc cgacagtcgt atgatgtttg ttctttccat catagttgac gaagacaaca
     2881 ccacatccga cattgatttc ttctcccagc gtagcatcac caacataagt caagtgaccg
     2941 actttagttc ctttgccgat ttgagcattt ttcacttcca caaagtttcc gatatggacg
     3001 ttttcgccaa tttcagcttt tggtcgtaaa tgtgcataag gtcccacatc tgcatgtttc
     3061 ttgacatggc tgctttcaat gactgagttt tcaatgacga cgtgatcctc aatcctactg
     3121 tctacgattt ttgaatgtga cccgatgaca caatcagaac caatgactgt atttccttgg
     3181 atttgtacac ctgcttcgat caatgtgtct ggaccgattt ctacgcctgc atcgatgtat
     3241 gtcgttgctg aatcgacaaa gcttacgcca ttgaccatat gcatctggtt aattcgcttt
     3301 ctcatgatct catttgcttt agctaacgcg atccgatcat tgactcccat tgattcttca
     3361 aaatcatccg tttgataagc agcaactgta tggccttctt cttttaagat ttcgatgata
     3421 tctgttagat agtattcacc ttgagcattg tttgttccaa ctttgcttaa cgcatcgaac
     3481 aaagcttcgt tgtcaaaaca gtaagttcct gtattgattt cttgtacaag agcttcttct
     3541 ggtgttgcat ctttttgttc cacgattttt tctacgatcc caatatggtc acggatgatt
     3601 cgtccatatc ctgttgggtt ttctgcttgt gccgtcaaga tcgttgcact tgcatttttt
     3661 ccttggtgat attcaaaaag attgttcagc gtttcagtag tcaataatgg tgtatcgcca
     3721 ctgataacta acgttgttcc tttttttcct tccaagaaag aagcagcttg caaaacagca
     3781 tgtcctgttc ccaattgttc tgcttgtaac gcatatttac tgcgttctcc tagttgtgct
     3841 ttaacttgtt ctgctccgtg tccaacgatc gtaatcactt catctggttt cgtttcactg
     3901 acccgattaa tgatatgttc taccataggt ttaccagaaa cgggatgtaa aactttatat
     3961 aattttgact tcatgcgggt acctttccct gccgccaaaa taattgcata acgtgcgtcc
     4021 aactgcgtca ctcctaaata tagtttgctg atttcagttt atcccagtat acccattttt
     4081 tcaacaaaac aagtgatttt cttttatccc agcgttttct ggataaaaaa atagatagac
     4141 cataacgggc ctatctattt ggtaaccggt gctctcctgc caactcctcg gtcacaagtc
     4201 acactactca taaaacatga aaagctgttt tttgaaatcg tttcttgtga cttagagtta
     4261 aacggtaggc ttacaacctc ttcattaaac gtttttcaat cagctgacct tcggtattag
     4321 ctcaataaac ggcaaaatat gaggagcaat ttttccgttt attgagctaa tactcaggac
     4381 aggacgctga tttcacaacc tctagataaa cggtgttcaa aagccagttt ctcggtatta
     4441 agccaaattt atgaaaaaat gtggaaagcc attttcacaa aatttttctt actactcgaa
     4501 actaagcggc tttttcacaa cctcttattc tgaaaaataa tttcctggca caaccgtaat
     4561 atttttcgtt tgagtatcta catctttgac ataaagcagt gacgtataat catcgatggc
     4621 gcgatgaccg ttgaattttg attcggcaaa tacagtgatg ccaacaagtt ctgattcgaa
     4681 ttcttcaatc aagctcttca ttccgttgac tgttccgccg cctttcatga aatcatccac
     4741 tacaaggaca cgtgaaccac gtttcaaact tctcttagac agttccatct tttcaattct
     4801 ttcagaagat ccagacacat agttgacact tactgttgat ccttctgtta tttttgagtc
     4861 ccggcgtaca ataacgaatg gcgcattcag ataataggac acagcttggg caataggtac
     4921 gccttttgtg gctacagtca ttacagcatc gattttttca ccaaggtatt tagaagcaat
     4981 gattcttcca acttgacgca gcagttcagg ttctcctaac aaatcagaga gataaacata
     5041 tcctcctgga agcagacggt cttgttctga tagacgttct gctagagatt caatatattt
     5101 tttcgcctct tcatatgaaa tttcagggat gaacaataca ccacctgcag cacccgggat
     5161 tgtttccaag gttccatacc ctcgttcttt aaatgttttc ttgatgattc ccaaatcttc
     5221 actgatagaa gatttagcag attcataacg ttcggcaaag cttgttaagg aaatcaattc
     5281 atgcgggtga ttcaataaat actgtgtcat gtcaactaga cgttcactgc gacgtacttt
     5341 cacctattcc aactcctttt aagaaatttt atcgttttac attatataag tttttcttca
     5401 aaaattcgag cattttatga aaaaataaga aaatagttcg gtatttatta aaataaaaag
     5461 aagcctataa aaaatttttc gtttttataa ggcttctttt ctttataatt tagttatttt
     5521 ttcttctgaa ggcaaccaat attcgtttga tgatatttac aatcaggaac aaagcaataa
     5581 agactaatgt gattgttgct cctggaggtg tatctagata aaacgagcta gttagtcctg
     5641 acagcatccc tagaaatcct acacaaacac tgatagcaat cacgacattg aagcttttgc
     5701 ctaatcgcat accgattgct gcgggaagaa ccatgattgc ggatatcaaa agtgcaccgg
     5761 caatcgggat catcacagca atagccacac cagtaatcac attgaacatc atcgacatca
     5821 aatgaacggg tagcccatcc acgtgggcgg tatcttcatc aaaagtcaga acatacatcg
     5881 gccgtttgaa taaaaagaac aatataccga tggcaacgaa taagattcct aaaaagatga
     5941 cttgctccca tgtaatggtc acgatcgaac caaacagata ggattggata cttgttgctg
     6001 aattccctcc gctcaaattc atcaatacta acgctagcgc caaaccacca gccattaaaa
     6061 tggcaatgga gatttctgaa taggttcggt agatcgttcg cagatactcc aatatcacag
     6121 ctgctaatac aacgactagc atggtcgtaa gtgtaggatt aaggttcaaa aagaatccta
     6181 acgctacccc tgctaaagag acatgagaaa gcgtatcagc catcagcgat tgtcttctga
     6241 tcactaaaaa cacacctagc atcggggcga ttccggcaat gaagatagcg gcaagaaacg
     6301 ctcttctcat gaattcatat gaaagcaacg ccattgagaa tcctcctttc tcaccagtcg
     6361 gatctgtctg tccgcatact gtttgatatc ttcatgatca tgggtgatca ttagaactga
     6421 tttcccatgg acatgcgcac tatgtcggag aagacgataa aattcatttc tggattcttc
     6481 atccatacct gttgttggtt catcaagaac aaataagtca gggtcagtag caaaaatacg
     6541 cgctaaactg attcgctgct tttgtccgcc agacaattca cctattcgcc gatttcgcat
     6601 atcccacatc cctacagctt ctaaagcacg ctgtacatgt tgatgatctt tttcagagag
     6661 acgcttgaac cagcggcctc ttggatatct tcctgaacgg actaattcga tcaccgtact
     6721 aggaaaacct gcattgaaag atgcaacttg ttgaggaata taaccgatac ttaatttttt
     6781 tccttcttga tttttctcag caatctttat cattcctttt gaaggtttca gtaatcctaa
     6841 cgatgctttc accaaggttg acttggctgc tccattttct ccagttaaaa tgacaaattc
     6901 acccgcatct acatgataag agacatcttc tagtacaggt tcatcatcat agtaaaaggt
     6961 caaatcagat atttcgatat aacgcacctt acaacctcca ttctaaaact taattgatac
     7021 tttcttttaa agctttcaga tttttttcca tgacagaaat ataattttcg cctgctttga
     7081 tttgttcatt agtcaagctt tctaaaggat tcaacacttc tagcttcact cctgtttcat
     7141 tcgacaatgt ctcagctacc ttcgaagagg cattttcttc aaaataaatc actttgatgt
     7201 tgtttttctt cacaaattct tttagctcag ctaagcgact tggtgtgggt tcctgatctg
     7261 gtgaaacgcc cgaaatcgct acttgtttta agtcgtattg attcgctaaa taagcaaaag
     7321 cagcatgctg agtgacaaat gtgcggtttg ttgcatcttt tagctcatcg gagtattttt
     7381 gatccagtgc ttcaagcttt ttgatataag catcggcatt cttcgtaaaa gtttcctttt
     7441 tatctggata tttttcgcct aattcttcag caatcgttct gacttctttt atagctaata
     7501 ccggatcgag ccaaacatgt ggatcgtatt catgtgaatg accatgccct tcttctgatt
     7561 cttcatgttc gtctgcttct tgcgattcta aacggtcgat tcctttagct gcttcgatca
     7621 tcaaggtctg tttgctatcc actgaatcct ttaacgaatc cacaaaaaat tccatgtctg
     7681 aactattata cacgaaaaca tctgcatcgg aaatttctgc tctttctttt gcacttggtt
     7741 cgaaatcatg cggctccgtt ccagcaggga tcaaaagttt cacgtttcct tcatccccta
     7801 cgatctcttt tgtaaaatca tacatcggat aaaaagtcgt aacgatcgtc aaatcctctt
     7861 ttttgtctgc ttccttgttc gtatttccac atgctgcaaa cagtaaagca cctatcatta
     7921 ccgtagctgt caaaagatat tttttcattt atatgctcct ttcaatgaac aaatcggaat
     7981 tttacgattt attaacgtga actaatgtat caaaataaaa aaacacagtc aatagaaatc
     8041 catcaatttc tgatttaagc atgtatattc ttgattaaat cgtaaaatgg atgagttgct
     8101 gctcatatct agccatttca ctacaataaa aaactgaaga ataccatttt aggattcctc
     8161 agtttttcat cacctatttt acttcttata ttttattgca gggttcgtac taaataaact
     8221 tcctcacaaa aaccttttaa cccattatat actctctgcg ctcttgaata atgttgacac
     8281 aaggcaaaaa cggttggtcc gcttccactc atcaatgcag catctgctcc gtacttgatc
     8341 atacgctctt tgatttgatg gatgactgga tgccgctttc ccgttatatc ttctaaactg
     8401 tttcccattt ttttgatcat ctgctgataa tcttgctctc ggatggcttc agcaagtcca
     8461 tggatgtctt gatgttggat ggcttgcgta tctacttctc taaacactgt acgtgtagag
     8521 acactcattc ttggtttgac caacacaacc cagcactgtg gcatagaagg taatacttgg
     8581 atttcttccc ctttgccacc gacaaatgcc gtacttccgt agacacagta aggaacatct
     8641 gtaccgactt ccaaaccgat ttctgccagt tcttctaacg aacagcccag attccataat
     8701 tggttgatcc ctcttagagc agctgcacag tctgtacttc ctccgcctaa tccagctgct
     8761 actggaatat tcttcttaac aaagatcttg acaccggttt tgatattgta tttatttttt
     8821 agcaataaag cagcttgata aacattgttc cgttcatcta ccggtaaaaa tgctttattt
     8881 gtttctacaa tgattttgtc ttctgttatt tcttcaaaaa atagatgatc tgccaagtct
     8941 acgctcgaca tcaccatttc caactcatga tacccatctt gccgtctata taatacatcc
     9001 agtcctaaat ttatcttggc tggcgctttt tcaatcaatt ccatcctctc acccattcga
     9061 aaagtctaag tcgcaagtat tctatcatag ggaaaaataa aagtatagct tatgtctgcc
     9121 tagtggttga ttttgtcatc gtttcaacag ctttcacaga attttctctt cataatccaa
     9181 acaagttcta gtgattctta taattctgtg agcacttctt ctgccaaact cggaacattt
     9241 cctttttctt ttcctatcag actgagtagc tgcatggtag caaatccgaa attacttcct
     9301 ttcctataag caaaataacg tccttcccaa tgatcgacat attttaattt cttttcataa
     9361 gcagactgga aggaatagaa aaaattacca taattatata cgatatgcat gactttttct
     9421 tgaaagaaag aaaaatctgt ttctccaaca ttagctaatg gagctaagcc tagattggct
     9481 tccgagtagc ccattttctc gtatacaata aacagattgg ctaacagata gtcagctaat
     9541 tcctcagatt ctttaggtaa gacccgcaac aaatcatatc ccgcctgctt cttcgtcatc
     9601 ggctgagcgg tgatgaagcc aacgactttt tgatctttac ggacaatacc gacaccgcta
     9661 gataatacat atgatttatc aaatcgtccc acagagaagt atttttctcg tgcaccttga
     9721 agccattttt gtgaaacttc atttacagct tgatgtaatt cttccggcag ttgatcgtac
     9781 caagtaaaat ggtatccttc tttcatcaaa cgttgatatt caagttgctg actcagccga
     9841 gacaagtctt ctgttgctag atcaacaatc cctgcttcac ctactttcgt gaagtgaaaa
     9901 cccaattcat gaagcatcac tacgtagctt tctgatatct tataaaacgc aagctgatag
     9961 cctaaacaat ctgcttgttt cataaactga ttcgttgctg cccgcatcat tttaggattg
    10021 ccaactggat ctcctaaaac aaagcatttg tttcctttga tctgataacc aaaaacgact
    10081 tgatcttggt cgtcttcttg gtaataaaaa taactgtaac ctgacaagcg aagctgatgg
    10141 cttgtagctg tgcctccgta tgtttccaat aaacggtcaa aacggtcttt ttcgtactca
    10201 ctgccaagct tcgtagtatc ttcagctaaa tattgataaa gcgccactaa agctagcaat
    10261 gagatgccca aaccaataag ccctgagaac cacacatcat ctgaaggaaa caaaataaaa
    10321 tgatttcctg ttattcgacc gactaaaaag cgtccgctgt gatagcctgc aattgcgtaa
    10381 acgataaaca aaaagccgaa caagctagca tcaaagatta tagctcccca agaataaacg
    10441 aattttgttc gataaaattc ttttctggat aaccacacag ctaacagaat caagacatat
    10501 accaatatca actgccaaga taacgtacgt gtgattgtat ttaaaatacc aaaagatagt
    10561 aaagcaatcg taggcagaaa tgcctttttg acttttactg aaagtgctct agcaaggccg
    10621 agtaataaaa agccaaccaa tagattcact gtctgatcta aaaaattgaa tgagaacggc
    10681 aataatactt gaaatagccg actgattgca gacagattag tgattgttga tagaagtacc
    10741 atcatgatcc ccgcaaaata gacagcactt acgagcacga tatgcgcaac cttttggaaa
    10801 aacagtcgcg gaatgttatc taaaaatcga ttgattttga ctcctgtatg tgtgatgaac
    10861 aatagaagtc ccgtcataaa aggtagaaca taataaaaca accgataata taatagccaa
    10921 actactgcta catcttgccg aacagacaat tgactcattc ctaagatcat caaaacgtca
    10981 aaagtcccca ttccacctgg gaccatcgtc agcattccta tcaatgtagc aatcatgaac
    11041 ataggataga tcgaaagcat cgaaatatct attctcatca acttgccgat gatcaaaaat
    11101 accgcaaggg ctcccgtcca ttgtccaaag gatgccagta gcagaccaag caagcctttt
    11161 ggataaaact cgaagaacaa tgtctttctc tttagataag caaaaaagaa aatgactggt
    11221 gctaaaaaac taccgccgaa aagccagatc caatagcttc gaaaaatact ttctgactga
    11281 ataaaaaaga catcaataaa tgtaagaaac gaccatacag acaaaccagt cagcataaat
    11341 agtgcgactt ttgatacagt ggctaaaacc atttttctat ttgtctgctt tccataaaaa
    11401 ctagcccgca gacttgctcc taccacacca ccaaaaccgg cgagattgtt gatcgtgttt
    11461 gttacccacg cagcaagaag aaactctttt ctttccattt ttggttttcc ttgtttttct
    11521 aagactctga tcgtcaccca atcatacaac aacatcggag tgacacccag cagtccagcc
    11581 agaatcatga gcaggatcgt catgcggctt tgttgtccca tcctttgcca aatatcttgc
    11641 caactcatgc cattagcaat atttgcgact tgatttgcca caaagactaa cacggagccg
    11701 aaaaaaatca gttttaaaat cagactatgt tcctttatcc attgcagcat caagtatcgt
    11761 ttatttttca cattttccca ttcctaactt gtatactttt gtgtcatacc ccaatgttat
    11821 cttatttatt ttaatttttc cactttagac ctgttctttg cttgcgatga tagaaaaaga
    11881 aagatatcct aatgctaaag gagaggataa catgaaagta ggatttatcg gcacagggaa
    11941 tatggcacaa gctattattg atggtttatt aaaaaaagga acagtctcga aagaagaggt
    12001 gttcgtttcc agtggacatt ttgaaaatgc acaaaaattt gctgctgaaa ctggcgtcca
    12061 tgcatgcaaa agtaatcagg aagttgcatc acaatcagat atcgttcttt tagccgtgaa
    12121 accaaaaatc attgaaaaag tattggaaga actaagagaa tatagccaga aagtcttatt
    12181 catttctatt gcttcaggaa aatcattaat agatttagaa acagcgcttg aagatcctca
    12241 ggcctccatc gtacgtgtga tgccgaatgt caatgtcagc gtaggagcag gcgtttctgc
    12301 gatttgcaaa aatgaagctg ccacggagga acagcttcag caagctacga tgatttttga
    12361 agcaatcggc agcgtctatc ctttagcaga atcagatttc agtacattca ttggccttgc
    12421 tggcagctca cccgcattcg tttacatgct gatcgatgcg atgggacgga ctggtgtact
    12481 gaatggtttg tccaaacaga cagccactga gattgctgca aaagcaatta tgggttcttg
    12541 tcaaaaattc ctttcaagtg ataaaaatcc atgggaactt gtagaccaag tttcttctcc
    12601 tggcggtaca actgttgcag gtgtagttgc cttagaagaa gcaggattca tcccagcaat
    12661 catcaaagga atcaatacga cgatcgagaa agatcttgag atgttgaata aaaaataatg
    12721 atagcaatgt tttttcgtgc cttacaaaaa aagaagagct gtggcttttg ccgcggctct
    12781 tcttttgtat tttttattat ttagcataat cagttgcacg agtttcccga ataacgatta
    12841 ctttgatgtg tccaggataa tccagttctt cttcgattcg tttacgaata tcacgtacta
    12901 aacgaacagc gtctaaatcc gagatttctt ctggcttgac cattacccga acttcgcgtc
    12961 cagcttgaac tgcgtaactt gattctacac cggcgaagct gttcgaaata ttttccagac
    13021 ttcttaaacg acggatgtag ttttctaatg attcacttcg cgcaccaggt cttgctgctg
    13081 agagcgcatc tgctgcagct acaaggacag agataacaga agtagcttcc acatctccat
    13141 gatgagaagc gatcgcgttg attactactg ggttttcttt gtacttggca gctaattccg
    13201 caccgatctc aacgtgagat ccttcaatct catggtcaag tgctttccca atatcatgga
    13261 gcaacccggc acgttttgct aattggatat cttcgccaag ctctgctgct aagataccag
    13321 ctaattttgc aacctcgacc gaatgcttca agacgttttg tccataacta gttctgaatc
    13381 gcagacgtcc gatgatcttg attagatctg gatgaagtgt atgagcaccg acttcaaacg
    13441 ctgcctcttc cccgtattcg cgaatacgtt catccatttc tttacgagat ttctcgacca
    13501 tttcttcgat acgcgctgga tggattcgtc catcttgtat cagtttttcc aaggtcatcc
    13561 gtgcgatttc tcttcggatt ggatcgaatc cggaaagaac aactgcttcc ggtgtatcat
    13621 cgatgattaa atcgatacca gtcagcgtct caagcgttcg aatattacga ccttctcgtc
    13681 caatgatacg acctttcatt tcatcattcg gaagggtaac gactgaaaca gtcgtttctg
    13741 aaacttgatc tgctgcgcag cgttgaatcg ctaatgataa taagtttttc gctttgcgat
    13801 ctgcttcttc tttggcacgc tgttcagatt cttttaccat tagagttaac tcatgattca
    13861 gctcttcttc agtcgatttc atgatgacat ctcgtgcgtc ctcttttgat aaaccagcaa
    13921 ttttttctag ttctttttgt tgatcttcaa tcattttttc aacgtctttt tctcgctctt
    13981 caattaattg ctgttttgaa ctaagtgctt cttctttact ttcaagtgag ttctcgcgtt
    14041 tttctaacga gtcatcttta cgattcagcg tttgttcgcg ttgaatcaaa cgattctctt
    14101 gagattttaa ttctaactta ctttctctca gctcactttc aacttcagaa cggtatttct
    14161 gattttcttc cttagcttct aacaatgctt ctttttttag agtttctgct tctttttttg
    14221 cactatcgat gattccagca gctgatgatt gtgcaccagc tatttctttc tcgtgacgtg
    14281 actttgcgac cataaaccct aaaccaagac cgacaattaa accgatgata gcgaggagaa
    14341 tgttgaatac cataaaatcc acctccatac tatctttttc atttttatca tctatacagc
    14401 ttctatgata aattattcta aactcgtcaa tatgcatctt cgcatatatg tgtacaaaaa
    14461 acatgcacaa ctttattcta atgtttgtac cccgtagtgt caactttaaa taaacaaaga
    14521 aacggctttc tacttattca ttaaacgatg gtttttaaaa taaatgacac tagtttcata
    14581 taggtaaacc tttaaattga gaaacttttc tattaaatta ttctttgtag atgaacagat
    14641 atttttattt tatgtaccca ctcgtataat taaataactc acgatttctt tattcaataa
    14701 aaaaacggca ggtcaaggag agtacctact cttcagacca gccgtttttg tttatcataa
    14761 tttttattct tctactaaag gcaattcttc ttgtcctttt tcatcttctg ggacagcgac
    14821 ttcttcacca ataccataag ctgctctaac tagagcagaa acttctgcca tcatttcagg
    14881 atgattagcc atatagattt tagcgttttc ccgtccttga ccaatccgat cttctttata
    14941 agaataccaa gcaccgcttt tatcaacaat gtctttttct actgccatgt ctagaagttc
    15001 tccttcttgt gagatcccta aaccatacat gacatctact tcagcgattt tgaaaggagg
    15061 tgctactttg ttttttacga ctttgatctt tgtacggtta ccaacaatat ctgtaccttg
    15121 tttcaattgt tctgcacgac gtacttccaa acggatcgtt gcgtagaatt tcaatgcgcg
    15181 tcctccagga gtgatttctg gattcccaaa catcacgcct actttttcac ggatttggtt
    15241 gatgaaaata gcgattgttt ttgttttgtt gatcgaacca gagagtttac gcaatgcttg
    15301 agacatcaaa cgtgcttgta acccgacatg tgagtcaccc atttcgccgt cgatttctgc
    15361 tcgtggaact aaagcagcaa ctgaatcgac tactacgata tctacagccc cacttgatac
    15421 taaagcatca gcgatctcta gaccttgttc tcctgtgtca ggctgtgaaa gaagtagttc
    15481 atcgatattc acacctaatt tttgtgcata ttgcggatct aacgcatgct cagcatcaat
    15541 gaaagcggcc gttccgccat ttttttgtac ttctgcaata gcgtgtagtg caactgttgt
    15601 tttacctgaa ctttcaggtc catatacttc gatgatacgt ccacgggggt aaccgccgac
    15661 acctaaagcg acatctaacg caagagagcc acttggaatt gtagagattt gttggtcgat
    15721 tttttcgccc aatttcataa tagagccttt accatagctc ttttcaattt ttttcagtgc
    15781 agcatctaag gctgctttac gatcatctgc caatacgata atcctcctta tatttacaaa
    15841 tcagagcaac atttcattat ccttatcata tcttttttag tgaaaaaaag caagaaaaaa
    15901 ccgaacaaat attcgcattt atcattttct taaaagctgt tttctcaaaa gatcaaatcc
    15961 ttgcataacc gcattatatc tgatagcagc acgatctctg gcaaagtgat atttttccga
    16021 aaccacgccg tctttagatg aaatagcgat ccatactgtt cctgcagatt gaccttctaa
    16081 cgtgtccggt ccagccactc cggtgaaagc tacactgtaa tctgttcttg ctatctcacg
    16141 tgcttgaatg gccatctgct ccacacattc tttgctgact gttccatatt ctgctaatag
    16201 ctccggatca atctccagaa aaccagcttt tgtttggagc gagtaggtaa cgaatccgcc
    16261 tggaaatact tcagatacac cagcaatgtc gcctaatgcg gcttggaagg cgccggctgt
    16321 caaactttcc gctgctgtta cagtctgctt gttttctttc agtagttcca ctacgacttt
    16381 tgctagcgaa ttatcatcgc cgtatccata aaaatattcc cctactcgtt cttggatttt
    16441 ttcttctaat gctaaaagag cttgattccc tgcctgaaca tcatttgttt tcactgtcag
    16501 tcgtaatgtc acttcatttg gtttggcata tggtgcgatc gttggattga tctgtgtctc
    16561 aatcagatct tttagctcag tgaccagtcg cgattcacca atcccataaa agcgcaaaac
    16621 tttagaaatt agtttttctt cagaaggaaa acgttcctct agtaaaggac gtacttgttc
    16681 cacaaacatt ggtttcaact cattaggcgg accgggaaga aggatatacg catgtgtatc
    16741 tgtttgataa aaagtcccta aagcaagccc tgtacgattt ggtagaggaa ccccgccttc
    16801 gataatctgg gactgttgca gattgttttt ggtcattttt cgatgggttg tttcaaaata
    16861 tgccaataat tttttgtatc cttccgtatt ttgtatgaga gacttaccga ggtgagccgc
    16921 agttgcttct tttgtcaggt catcttctgt cggtcctaat ccgccacata agagtatgag
    16981 ttcacttctt gtttcagcta gagcaatcaa ctcttctaaa cgctgctgat tatctcctac
    17041 aactgtttga taataaactt cgatacctag atcagctagc tgttctgaca agaaagtggc
    17101 attcgtattc acgacttgtc ctagtaaaag ttctgttccg acagctatta tttctgcttt
    17161 cattacttcc cgctccctta tatagtatac gctgtatttt tatatttttt tatttaaaaa
    17221 gagggcaagg agctgtgaca aaaatcttgt cacagctcct tgttccaaat aaacagtgtt
    17281 ctcctgccaa aacctcggtc ataagtcaca atatacataa aacatgaaaa gctgtttttt
    17341 gaaattatcc cttgtgactc agagttagac ggcaggctca caacctctcc actaaacgat
    17401 acttacattg atcctttgaa cacaccgctg tttttgatga agtaatccac acctgaatag
    17461 atagtaaaga tcagacaagc ataaagcatg atttgatcta ctggaatacc gatcaggctg
    17521 aatgggatat tattgataaa cagcaagata atagcaacca tttgtgtagc cgtcttgact
    17581 tttcccggcc aagctgcagc catgacttct ccatgttcga caagtagtaa acgcaagcct
    17641 gtgacggcta attcacgaca gacaatgatc gctactaccc aaccaggggc ctttccttgg
    17701 tcaaccaaca tgataaaagc agtcataact agcattttat ctgccaacgg gtccgcaaac
    17761 ttgccaaaat tagtgacaag gcctcgcgca cgggcaattt ttccatctaa ccagtctgtg
    17821 atactagcta aagcaaaaat aactgcacct actagctgtg tgacagccag tgtggtgtct
    17881 cctacgttaa attgtcccca atctaaaggt aaacctacaa ccaaaatgaa aatcgggatc
    17941 atacaaattc taagtacagt taatttattt ggtaaattca cactatcgac tcctttcttc
    18001 cctattctac gtgactatca gcgaagtttc caccgatatt atttactttt ccaaatgaat
    18061 ttattcaaag gagtattttt attgaaattg atctttttga attaacgaag aaggaccgcg
    18121 acacagattt gtcacgatcc ttctttttta tacctacaag taaacagatg cgtaaactaa
    18181 tttatacgat cattctgcgt attgaagagt aaggttcaca ttttttggac tctgattgtt
    18241 ttcccctgga ttaactggta cggattctcc attcgctttg atcgttgcat tgcttgctgc
    18301 accaatcgtg atggtcgcat ttgttgctgt ttccggtaac acggtactct gtgtttcatt
    18361 tgctgccagt gtcccttgat aaacataggc attgtctact agcacaccta accatacacg
    18421 attgacagca gtaaattcaa gggttacagg cttggtagcg tctgtgatat ttactgcgat
    18481 ggcactgcca gaatcttctc ctgtagctat tgccattttc ttttcttcac ttgattttgt
    18541 tgttgattct actgtagaag actctttggt ctgcgtgctt tgctctgagg aagaggactc
    18601 ctcgactgat ccttctactt taagcgaaga agctgtgtct ccgattattg gatcagcttg
    18661 acgatcttgc caagtcatat agcccacgac aacgacaatc gccaaagcaa ccaatcctaa
    18721 taaaatgaca ggaagacttg tccaaaagcg acttggatgc gattcttcct gatgcattgc
    18781 ttttctagaa gtacttactg tttcaggctg cggacgttca gggaccgatc ctgtcaattt
    18841 gcttttccca tcaaaaacat cgactaaatg gtctccatct tctcctacag cttcagcgta
    18901 ttgccgaata aatgcccgca cgtaaaattt accaggcaat tggtcaaaat catcattgtc
    18961 gattgcttcc aaataacgtt tttgaatttt tgtaatctgc tgtaattcat ctaaagatat
    19021 ttttttattc aggcgtgctt gccgtaattt ttctccgatg gtttcaaaag ccactcgttc
    19081 atcttctcca gtctgtttga ttctccgaag catctgctgt tgcaacgaaa gacgttatgt
    19141 ttgggtgctg tgacaaaagt catgtcacag ctcctttttt ccgaataaac attgttctcc
    19201 tgccaactcc tcggtcacaa gtcacaatat ccaataaata taagaagctg ttttttgaac
    19261 ttgttccttg tgactcggag ttaaacggca ggctcacgac ctctttctta aacggtgttc
    19321 aatcagctgt tcctcggtat tagctcaaaa accggcaaaa tataaggaga tatttttccg
    19381 tatgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttcct taaacggtgt
    19441 tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacattaa gaagctgttt
    19501 ttacttggct gttcttatta cttgatgcag ggcagctttt tcacaacctc ttggtacacg
    19561 gtgttccaga agtagcttct tcggcaataa gccgaaatat cacaaaaatt tgagaagcaa
    19621 ttttcgtaaa ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctctttgatt
    19681 acttcgtttg attttatttt aatataagtt gattaaaaga gagcatgatt tgttttattt
    19741 tctcactaat ttttccaaca caactaagaa taccataaaa gctgattttg gaggaggttc
    19801 ctactacaag tctaaaacga aatttaagac ttttcctcat aaaatactgg tttattttgg
    19861 tctcatgtaa aactcgctga aggcctcttc acttataaat gcttccccag ctgccagtac
    19921 gtctttcatt tggatcgaat cgatgatttc aggaagatca aataaagttc gatcgccaaa
    19981 taaactttgc gtaaattgat tggcaatata ttcgatcgaa tttaatgatt ggaaatattg
    20041 tccaagcatt ttcttcttca gcaaatcgag atttgtttct gacacttccg gatcatcagc
    20101 gaatccaaga atgatttctt tgactttttc tgctgctttc tctggttcgt ccgtatctcc
    20161 gctgaagtcc gcaaagtgaa attcacgatc caagctgaat tcaaaaccaa agctgtcatc
    20221 gataattcct tgattgtaca tcgctagata gtttcgagaa gtattgccta aaatcatttg
    20281 gaacaacaga ttgattgctg ttttatagcg taacagttct gttccttctt gcggcaatgt
    20341 gtctaatcct ttaataccaa gtacaaattt atctcgtgta atggctgctt ccaatgcact
    20401 ttgtttgatg atttcttttg tgttttcagg gaaataacgt acaatttctt gctttggagg
    20461 gaaatttttt gcttcttggt tttcccgaat aagtttcatc aatttctcag gctccatctt
    20521 accaactaca aataaaacca tattgcttgg ctgataaaac gtccgatagc acgtatacaa
    20581 gtcttgagct gtgattttgt cgatactttc aaccgtgccc gcaatatcga tatgcaatgg
    20641 gtgtgtagga tacaagttat ttagtatgcc aaaaaacatc cgccagttag ggtcgtcttc
    20701 atacatttgg atttcctgac cgatgattcc tttttccttg tttacagttt cttctgtgaa
    20761 gtaaggtgct tgtacaaaat cgatcagagt cgtcagattc ttttctacct gatccgtcgt
    20821 agaaaacaga tagctggttt tcgtaaaact tgtgaaagca ttggcggaag ctccttgttt
    20881 cccaaataat tgaaagacat caccatcttc tttttcaaac aacttatgtt ctaaaaaatg
    20941 ggcaattcca tctggaactt tcaccttttc tttttcacca tagggaataa attcgttatc
    21001 gatcgaacca taattcgtac tgaacaaacc atatgtcttg tgatagtcat ttttaggcaa
    21061 caaatacacg gtcaaaccgt ttggcagtac ttcatgataa agtgtttcat tgatttgctc
    21121 atattctgtt ttattcattt gcatttcctc ctgctaaaaa gaagattgcc tgcaattcga
    21181 tttgctcagc tacttgttgt atctctttga tcgttacgct ctccatacgt ttcaaccatt
    21241 cttcatcaga taattttgtt tccggcagcc agctgtccaa aaaagaagct tcgattgccg
    21301 cttgcggaga gtctagagaa agcagatatt ggtttctcag cattgctttt gtctgagaca
    21361 gttccaactc tgttatttca ccatttcgca aactttctaa ttgctcgtga atcagatgaa
    21421 gtacttgatt tcggttcttt tcatcgatac cggtttggac actcatgaat cctcggaacg
    21481 tatccacgct actagaggca taataagcta gactttcttt ctctctgaca ttcatgaaaa
    21541 gtttagagtg aggaaaaccg ccaaataaac cgttaaagac cattagcgca aaacgttctg
    21601 gttcatcgta atacacattt gtctgataag ctaggttcag ctttgcttga acgattggct
    21661 cacgtacttg tcgttccttc acgatattca cttgaggttg ggtataaaac agatctggat
    21721 aaactgtttc ccgcgactca aatggtaatt gcccaactgc ttcagctgct ttgtccggat
    21781 caacatcgcc gacaacaaaa atgtcgattt gatcatttgc catcatttct tgatagtagg
    21841 acgctagcga actagcagtg atcttgtcca aatcttcgac tacgcccaac cctgggatct
    21901 tttgttccgg gctgttttca aaataaagtt cttgcaaggc ataggaagca aaagtttgtt
    21961 tatcttcctt caaactttcc aaatatagac gcagattgtt cttttccaag tcaaacgtca
    22021 actgatcaaa ctgatgattt ttaatatttg gataaaataa aacctctttt aaaaaatcta
    22081 ccgcttgaga aaaaaggttt ggatcattga catattcacc attcacaaaa ctgatccctg
    22141 cgtttagcca atgtaaattt cctttttttc taactgataa tccaaagctt gcgccataaa
    22201 gctctgcaag ttgcgcactt aactttgtct gatccgaata attcaaacta tttgtttcca
    22261 acatgctgct gagaagtgaa cgtttcgtga tgacttcttg ttgcagacgc gcagtaaaac
    22321 ggataaaaat ccggatcgtt ttgtattgtt ttgttggtaa gacatgaaga ttcacgcctt
    22381 ctgccaattg atacaccata tttatatgct ccttttcaca gttgattgcc cgcttactct
    22441 tttaatgggt caacctgcct tttttactta ttcctctgct tgaaatttat aagttcaggc
    22501 aattcgccgt tttttctaaa tgatgttaca ctttatagta tagcatagat gttttttatc
    22561 aaaagaaaca gacggctaaa agcgtatatt gtttttttat tttttatttt tcgatatact
    22621 aaagacaaat aaaagaaggg agggtcattc atgatcaaag aattcaaaga gttcatcatg
    22681 cgtggagatg tcttggatct tgctgtcggc gttgttattg gtagtgcgtt tacaagcatc
    22741 gtaaaccaag tggtgcaagg cttgatcaca ccactaatag gttggtttgt cgcaatgatc
    22801 acaggtactt ctgatttaga aggagcactt tcaattcttg actggtcgcc gacaaaaggc
    22861 gtcacatttg cgtttggtaa cgttattagt gctatcatta catttctgat cactggtttt
    22921 gtcttattct tagttgtcaa agcggcaaac cgtgcgaaga atttacgctc caaagaagag
    22981 caagtggaag tggaagaaac tcctactgca gaagattatc ttagtgacat acgcgatctt
    23041 cttgccaaac aagtggcaga agacaataca catccagtag agcaaacaaa tctttccgat
    23101 acttctaaat aataaagaat ctaaaaaaaa gaaagccgat tttcagtgtc ggctttcttt
    23161 ttttagattc ttaacttgct acttgttcct ttttcatatc gatatgtgga atatcatctt
    23221 ccaaatacac atctgagatt gccttgaatc caaacgactc ataaaaagct tgtaggtatt
    23281 cttgtgcgcc gatcacgatt ggtttttctg gaaaattttt ctggatcatg ttaatcgttt
    23341 cttgaacaat ctttcttcct aaaccatctt tacggttgtc ctgatgaaca aggacacgtc
    23401 caaaatggat actctccgct tcttcatata ctcgcgtgta ggcaaggatc ttcccatcag
    23461 tatcttgaaa aaacgtatgc caagcttctc catcaatgtc atcaacttct tgataaggac
    23521 actgttgttc cacaacaaaa actgcgattc gttctttgac gatcgcgtaa aactctgctg
    23581 ttgacaattc ttcaaatttc tttacctgat attccactaa gcaaacgccc cctgcttttt
    23641 tattcttagc ataccgattc gcacacaaaa aagcaaacga accttacgaa tctcaggctc
    23701 gtttgctttt taaactgctt cagttaaaaa ttcgatctca actgtccgag taagtacatc
    23761 tgaatagctg taagatactc gttcaaaaga attttcttca ggatccaaat caacaacgaa
    23821 tactgaagga taggtttctg ttaaaacgcc gcggcgttct gtttgacgct ttcttcctgt
    23881 ctgagcaacc aacatgattg ggctgccgat acgaccttct aaatctttct tgattgaagc
    23941 taaagttgtt ggcatacagt tcacctctac tatcaactat accacatatt tttaaaattt
    24001 tcaagtttac cataaaaaaa aaagaaatgc aataaaaata aataagaagc tcattaagat
    24061 ttttctaaat tattcctcat tataaaattt caagtgtttt tttattttga gttttactct
    24121 cccgtaagcg ctcattactt gcttgtgatc cattgctaaa cattcagcca tgtctacaaa
    24181 atcaacgcct tgcatataat aatgaaaaat tttagcttct atttttgaaa actggatttc
    24241 gcaattttcg attgcttctt ttaataaaag atgctgaatt gctaaaggtt cttgcaatgt
    24301 gatctctgct aaaaagtctg gtccttctgt ctctattttt tggtcgattg agacttcatc
    24361 tttttgatca cgacgcttca gcgcgttctg cttcctcaac agagaataga tttgattgct
    24421 tataacgctt ttataataac ttccaaagct aacacctttg cttttttcgt aagttctcaa
    24481 tgcctgatat aaagcgatcc ttgcttcttg ttcccaatcc tcagaaacat aatttcttaa
    24541 ataataggtg tttttcaaac gtagtaaaag tggaaaatat ttttgataca gggcagcaaa
    24601 tgcttcttcg tcaccattac gtgcagcgcg caataactcc tcagacataa aataattcct
    24661 ccataaattc gtttatggag gaatcttatc atcaatgatt catcgaactg attagaacga
    24721 tatgttattt attttttctc attaaatcgt ccaatttttg ggacaatttt tttaactgct
    24781 cttcattcca tggagaatta cgtcggaaat caataaaatg catatcttta ttttcttggg
    24841 ctattgtttt ttccgttttt tttatcgtct tatataattc ataagcagaa gttcgtaatg
    24901 ctccttggga aaagatcatc cactgttctg ctaaatcgct tgtcgccact gtcacttgtg
    24961 tcaaacggtc attcagctga ccagcgattc tctcgatata actgtcagcc gtttcgtctt
    25021 ctttcgtaaa aacaacatcc aactggtatt tcttataatt ttgttggatg cccggaacta
    25081 gttgggcatc gaagacaacg atgacttcca ttccttcata tttcgcataa tttgataaac
    25141 gatgtaaaag agcttctctt gcatccgcca ttttgtcctg ctttttaagc tggacaagtt
    25201 ccggccacga accaatcatg ttgtagccgt ctacaatcaa cagctgtttt ttcatggtct
    25261 actccccctg acgttgggaa aaagcctgat acataagcaa accagcagca acactggcat
    25321 ttaagctctg gacgtgcccg gtcatcggga tcgtcagcaa ttcatccatt tcttttttca
    25381 atcctggact cattcctttg ccttcattac caatgactaa agcaattgat cctttggcat
    25441 tccatttttg gaagtttgtg ccggtcatat ctgtgccaaa tacccaaaat cctgtttctt
    25501 tgagctgttg cacagcttgt accaaattcg tcacgcgtgc aacagggaca tgttcgactg
    25561 ccccagtgga agtctttgtt acgataggtg tgattccaac tgctcggtgt tttggtatga
    25621 tcacaccatc gacgccactt gcgtctgctg tccgtaaaat cgacccaaaa ttgtgtggat
    25681 cttcgatgct atccaaaatc aaaaagaagg ggtcttcttt tttctctttt gttcgttcaa
    25741 tcagttggtc aagggataaa tattcataag gtgtaatcgc aagaacgact ccttgatgca
    25801 cgccgtgatc gctcaaagtt tctaattttt gtttaggaac ccatttgact ggcacagctt
    25861 gttcacgtgc gatttctttc agttcttcta ctttttctcc tcgtgaatct tcttgaagaa
    25921 agagtttatt ccctcgccct tgttgtagcg cttctactgc ggcatggtgc ccaaagacga
    25981 aattatcttc taatgcttct tcattttgat cagaaactga ctgatttcgt ccagtttgct
    26041 ttttatttga atatctattt tgttttttgt cttcaaatcc acgtttcgat tgatgtttgt
    26101 ttgttctttc tttacccatt tttttcacca actttctgaa tacaccagcg gatcaattct
    26161 tccatccgct cggtttgttt tgtcaaatgc agatagccca tgagcgcttc gaaccctgtt
    26221 gcgacccgat acgtcgtaac atcagcattt tttgcgctcg tatggctttt agcatttctt
    26281 cctctgcgat aataaagttc ttcttcttct gtcaaaagat gttcttccag catttgctga
    26341 atcaaaaagg cttgtgcatt cgcagaaaca taattcgttg ccgcacgatg cagcttattg
    26401 ggttttgttt ctccttgatc aatcaggtgg tcacgaacat agatttcgta aatcgcatct
    26461 ccaacataag ccaaggctaa accgttcaat tgtgtgtaat ctctcatgct tctcttctcc
    26521 atcgggttcc ttgcggtgta tcttctaata agatcccttt ttctttcaac aaatcacgaa
    26581 tttcgtcgct tcggacaaaa tcacgattgc ttcttgcttg atttcgttct tcgatcaatt
    26641 gttcaatctc ttcatctaat aactcttctg ctgtaaataa aataccgaag atcgaaagcc
    26701 attgcgagaa taaagcttct ccttgtgcca agacagcggc agaaactttc ggctggtcta
    26761 gataagtgtt cagccactta gcaagctcat agaccactgt gatcccattt gctgcattaa
    26821 aatcatcgtc catttcttcg atgaatcgct gttctaaatt ttctaacaaa gcgattttat
    26881 ccgcatcatc tgccaaattc tctgaggcag tttcttggcg gaatttcaaa ttgtcaaacg
    26941 tattcttcag tcgttgtaaa ttggtggctg cttctttcat cgtcgtttca ctgtagcgga
    27001 tcggacgacg gtattgtgtg gtcgccatga agaaacgcaa gacttgtgga tcaacgtttt
    27061 tgatcatttc atgtacagtg atgaaattcc ctagagattt actcattttt tcatcgtctt
    27121 cgccaatcgt tacatagcca ttgtgcatcc agtaattagc gaattttttc cctgttttcg
    27181 cttcactttg tgcgatttca ttttcatgat gaggaaattc taaatcttgc ccgccaccat
    27241 ggatatcgat cgtttctcct aaatgttttg ttgccataac ggaacattca atatgccaac
    27301 cgggacgtcc tttgccccat ggtgaatccc aagaaatctc gccaggtttt gcttgtttcc
    27361 ataaagcgaa atctaatggg tcttcttttt gctgctgttc ttcccctgta cgctgacttg
    27421 ctccgacttc cagttcatcg atggattggt cacttaattt cccataattt tcaaatttac
    27481 gggtacggta atacacatca cctgaagatt cataagcata gcctttttca accaacactt
    27541 gaataaatgc taaaatatct ggcatatgat ccatgactct tggatgcagt gtagcaggtt
    27601 cgacattcaa cgctttcgta tcttcttcaa atgctcggat gaaacgatca gcaacttctg
    27661 gtgctgtaat acctaattcg tttgctgtac ggatgatttt atcgtctaca tccgtgaaat
    27721 tcgatacgaa atcgacttta tagcctcgat attcaaaata gcgtcgaatt gtatcgaaag
    27781 caatggtgct acgagcattt ccgatatgga tataattata cacagtcggt ccgcagacat
    27841 acataccgac atgtcccttt tgtataggga tgaattcttc tttttttctg gtcaatgtat
    27901 tgtagatttt tatcattctg atctcctcac tttttctcct ttgattcgaa cgacttttgc
    27961 tggtactcct accgctgtag cgtcgtctgg aacatctttc aacacaacgg cacctgcacc
    28021 gattttggcg ttttttccga tcgttaccgg tcctaatatc tgcgcattcg cagagagcat
    28081 ggctccttgt ttaacagttg ggtgtctttt acccgtttct tttcctgtgc cgcctaatgt
    28141 tacgccatgg aataaaatca catcgtcctc gatttctgcg gtttcgccaa tgacgattcc
    28201 cattccatgg tcgatgaaca cgccagttcc aatcgttgct cctggatgga tctcaacacc
    28261 agttaggaaa cgccagaatt gagcattgat cttcgccaat aaaaagagcc ggtgctgata
    28321 taaaaaatga gagaaacgat gccaaaacaa ggcatgaaaa ccagggtatg tcaaaacgat
    28381 ctcagctgtc gaacgtgcag ccggatcgtt tctctttgct gcatcaatcg ctcgggtaaa
    28441 ccaactcatg actttccact cctttcgtaa gaattttatg taggaaatca tcttcttata
    28501 aagaaaagaa gcgcctttta aataagtttc cttatcaaaa gacgctttcc gcgtggttcc
    28561 acttttcttc ataatcaatg attatctctg acagctaacg cctgttatac gttcttggct
    28621 actttgtttc ctataaaaca gtttttcctg ttttctgtgt ttcgccaaga ccactcccag
    28681 aggcatttcg gcaaccattc attgatacct tgcaccaccc ggtatctctc ttgtaatgat
    28741 cagctgctta cttttttctg ttcatcgtgt ttagttcagt acttgattta aatgtgctaa
    28801 tgatttttca cgaccaagca attcgatcgt ttcaggcaat tctggaccat gcatttgtcc
    28861 tgatacagct acacggattg gcatgaaaag attttttcct tttacgccag tttctttttg
    28921 aacagctttg atggcagctt tgacagttgg agcatctact acttccattt gttctagctg
    28981 tgctttgaat gcgtggagaa cagtaggaac tgtttcacca gcaagtactt cttttgctgc
    29041 ttcattcaat acaggatgtt cattgaaaaa tagcgtagac agatcaacga tttctgcagc
    29101 atagctcatt tgaggttgat acaggctaac gatcttcttc acccattcga ttgtcttttc
    29161 atctgggttt tctgctacac gtccatcagc gattagataa ggcagacaca tgtcagtaaa
    29221 tgcatctaga tccattgctt tgatgtaatg gttgtttacc cattgcagtt ttttcgcatc
    29281 gaaagctgcg ggtgatttac ttaggcgttt tggatcgaac attttgatca attcttggtg
    29341 ttcgaagatt tcgtcttcgc ccacaggtga ccagcccaat aatgcaataa agttgaacat
    29401 tgcatctggt agatagccta attcgcgata ttgttcgata aattgcaaga ttgattcgtc
    29461 acgtttgctt agttttttac ctgtttcact gttgatgatc aatgtcatat gtccgaaggt
    29521 tggtggtgtc cagccaaatg cttcatagat catcagttgt tttggtgtat tggcaatatg
    29581 gtcatctcca cgtaaaacgt gagtgatttt catcaaatga tcatcgaccg caacagcaaa
    29641 attataagtt ggcataccat cgcgtttttg gatgacaaag tcgccaccga tattatcgga
    29701 ttcgaacgaa atagcgcctt tcaccatatc tgtaaatgcg tattctgtat tgcgaggcac
    29761 acggaaacgt acaacagatt ctagaccttg tgcttctttt tctgcttgtt cttcaggtgt
    29821 caagttcgca catgttcctg cgtaatgagg catttctcct cttgcgcgtt gtgcttcccg
    29881 ttctgcttct aattcttctt ctgtgcaata gcatttgtat gctcgattgc tagcaagcag
    29941 ctgatcaatc agcggttgat agatttcttt acgttcagat tgacggtaag ggccgtattc
    30001 tccaggattt tcaggagatt catcccaatt cattcccaac caagcaaggt tttccaactg
    30061 gcttttttcg ccatcttcga tattacgttt ttggtcagta tcctcaatac gaataataaa
    30121 gtctccatca ttgtggcggg caaacaaata attgaataat gcagttcttg catttccaat
    30181 atgcaggtgc ccagttggac ttggtgcata gcgtacgcgt actttcgtca ttttcttcct
    30241 cttctttcaa actagacttt gactaacagc aaaaattccg tcagttacgg cttctcaaat
    30301 atcatttatt taaagccact cagcacaaat tttacctttt ctaaggtaaa tagtaaagcc
    30361 aactggttga atttccttac cagttggctg aatttctttt ttattttgat tgtttagtct
    30421 ttttctctgt ctctttttta ttgtcgattc ctcgtcctgc atgtgcaggt ttagcaaaga
    30481 tcatgcgtcc agcagctgtt tgaagggcac ttgttacaac gacttcgata cgttcattca
    30541 tgtaatgctg tccatcttct acgacaacca tcgtgccgtc atcaaggtag gctactcctt
    30601 gttggcgttc tgtcccggct tttactacta gaacattcat cgtttcacca gggatgacga
    30661 ctggttttac ggcatttgcc aacgcattga tattaagaac aggtacattt tggaattcag
    30721 agactttatt caagttatag tcattggtta caatgacacc atctaaaagc ttagccaatt
    30781 tgatcaattt gctgtctact tcactgatat cttcaaagtc tccatcgtac atttcgacag
    30841 atataccctc ttctttttgc aaggcattta ggatatctaa tccgcgacga ccacgcacac
    30901 gtttcaaact atcccctgag tccgcgatgt attgtagttc gtaaagaaca aaattaggga
    30961 tcatcaaggt tccttctaag aatccagtct ttgtaatatc ataaatccga ccgtcaatga
    31021 tcacgcttgt atccaagatt ttatatttat ggaaattttc tcctgatttt cgttctaaca
    31081 gttgactctc aacttgacta gctgtttctt gctcgatttt tttgcttcgt gaaccaaaaa
    31141 ctttcttcca ttcttcgatt cgtgtcgttc ccattctcaa tcccagatag cctaagatga
    31201 tcatcagtaa aatcggcaat acactattga cgaatggaat ttccatattg taaagagggg
    31261 tcgagatcac aacacctaaa gtcaatccga tgatcgcacc gatcgcaccg aagagaagat
    31321 aagtcaagct gacctcactt agtttttgtt ctacttgttt gataatagct gagatatgtt
    31381 tggccaacag caaagataat aaaaagaaaa taagtgcgcc taacaggctg ttggtaaatg
    31441 tattattcag ccatccgttt gactgttgac cgattgcctg ccaagctgag ggtaaaaatg
    31501 aaatacccaa gcttgcgccg acaatgatca tcagcaaagt aatgacacgt ttttgcatcg
    31561 tttttcctcc gtttcgacaa tcatttatta gttagcggaa cacacgcttc aacgtttcag
    31621 ccaaagttgc tactccaact atctcgattc cttttggcgc ttcccagtta cctaagttgt
    31681 ttttaggtag gtatactttt gtgaagccga gtttttggac ttctttgact cgttgttcaa
    31741 tatgactgac acgtctgatt tctccagtca gtccaatttc accgatgaaa cattccgttg
    31801 gctgtgttcc tttttcttta tagcttgaag caatactgac agcgatagct aaatcaatcg
    31861 ctggttcatt taattttacc ccacctgcag cttttaagta agcatcttga ttctgaagaa
    31921 gcaacccagc tcttttttcc agcactgcca tgatcagaga tacccgattg aaatcaagac
    31981 ctgttgttgt tcgtttggca tttccgaaca tagtaggtgt gactaatgct tggatctcta
    32041 ccaaaattgg acgtgtccct tccattgcca caacgatagc agagccagtt gcaccgtcta
    32101 ggcgttcttc taagaaaatc tgtgaaggat tagcgacttc ttccaatcca tgctcatgca
    32161 tttcaaaaat cccaatctca ttcgttgagc caaatcggtt ttttactgct cgcaaaatcc
    32221 gaaaactatg atgttgttca ccttcaaaat ataacaccgt atccaccata tgttctaaca
    32281 ttcttgggcc ggcgatcgag ccttctttgg taacatgacc gacgataaaa atggctatcc
    32341 catttgtttt agcggttttg agtagttctg cagtggtttc tcttacttgg ctgacacttc
    32401 ctgccacact tgtgatgtct ggctgcgtca tcgtttggat ggaatcaatg ataacataat
    32461 caggggagat atgttcgatg gcacgactga tttcgttcat atctgtttca gcgtatagat
    32521 aaaattctgt gttgatactt ccgaggcgtt ccgcgcgtaa ttttatctgc tctgcgcttt
    32581 cttcgccgga tacatataag acttttccgc ctatggctgc taattgctga gaaacttgta
    32641 acagcaaagt ggatttcccg atacctggat cgccaccaat caagaccata gaccctggca
    32701 ccacacctcc gcctaacaca cggttcagtt cttctaattt tgttttgatc cgaggttctt
    32761 ttttagggac gacgtctgcg atttttgttg gttttgcttt ttctcctgtc aaacttgtcc
    32821 gagttctgcg gtccgttgta tcttgttcga tttcttcgac catcgtattc cattttccgc
    32881 aatttgggca gcgtcccaag aacttaggcg acacataccc gcaattttgg caaacaaatt
    32941 gtacttttga tttttttgcc attgtaccgc ctccttttat cctaatcatg agacattaaa
    33001 tcaagcttaa gtatctttaa cgttttattt actggaagaa ccaaatccgc cttgacgttg
    33061 agcagtcgtt tgatcttgat ctgcttttaa aaacggcaaa aagatccctt ggccgatacg
    33121 ctcgcctttt ttgatcacta catccttgaa accaaagttg gtgaattgga acatgatatg
    33181 gccttcattt gcctcattat tgtaataatc actgtcaatc acacctactc cgtttgtcaa
    33241 cactaaaaac cgcttcaacg gattgcttga acggttagca agctgcaaaa attcatcttc
    33301 tcccatgtaa gctttgattc cagtaggcac gagggttggt ttcaattgtt tttcattttc
    33361 tgatacatct tttttcaatc catctgcgac tgtttccaac ccctttttcc agcctgcttt
    33421 ccagatactt ggaatcacga cctcttctgc tgcttcaaaa tcatagccag cagcatgatg
    33481 tgtagcgcgc tgcggtagat tgatattttg atctttatat ttagaaataa cttcaaatcc
    33541 tctttttctc accggttttc ctccctaaat aaactactcc tattctaaca tatccgaggc
    33601 tgtaaaagac tttctttaca aattcttcaa gaaattctgc tatcttaaag taaaccatta
    33661 attcgttctt tttccaaacg actgcaagtt gtacaaaaaa actatttttt acatgacttt
    33721 actcgtcaaa acaagtagaa tagaacgaaa ttaatgagtg gatatttgat tgaggtgtca
    33781 catgcgtaaa gaaattaaaa atgcaaaacg aattattgtc aaagtaggaa caagtacatt
    33841 gatttatccc aatggaaata tcaatcttgc ggcaatcgat cagttagctt ttgtcctttc
    33901 tgatcttaga aatcggggaa aggaagtgat cttagtctca tcaggagctg taggcgttgg
    33961 tatgcataaa ttacatttgt cagaacgtcc gactttgatt cctgaacagc aagctgcggc
    34021 ggctgttggt caagccgaac tgatgaatat ctataatcaa agattcagtg tatataatca
    34081 acagacggca cagatcctcg tgacaagaga cgtggtagat tatcctgaaa gtcgtaaaaa
    34141 tgtgaggaat acattggaac agcttttgat tatggacacg atccctatcg tgaacgaaaa
    34201 cgatactgta gcagtggatg aattagatca tctgacaaaa tttggcgata atgatcagct
    34261 gtcagccatc atggcaaaac ttgtcgcggc agaccttttg atcgtccttt ctgatatcga
    34321 cgggttttat tcagataatc cgactacaaa tcccgaggct cagatgtatc gctatatcga
    34381 taaaatcgat gaaaagattc tggcacaagc aggtggtgct ggtagcacat ttggaacagg
    34441 cggtatgcag agtaaattaa aagccgcttc taggatattt gaagcaaaca gcgcaatgat
    34501 tttagcaaat ggaaaaaatc cggcaattat ttttgatatt cttgcaggaa aagaagtagg
    34561 tactttattt aaggaggact tgaaatgact gacttgatgc aattaggaca acaggcaaaa
    34621 gatagcgcgc aacaactagc acttatgcct acaaaccgca aaaatgagct acttgttcaa
    34681 atggcacaaa ccatcaaaaa gaatcaacaa gcgatcattg aagcaaatca taaagactta
    34741 gaaaaagcag tcgaaaacaa tatcagtgaa acgatgcaag accgcttgca gctgacacct
    34801 gagcgaatca atgccatggc tacagagatc gaaaaaattg cttcgttaga cgatcctatt
    34861 ggaaaagtag atgagatgtg gacgaacacc gacggattga gaatcggcaa aaagcgcgtg
    34921 ccactaggcg tgatcggcat catttacgaa tctcgtccaa atgtcacgac agatgcagct
    34981 agtctagcat tcaaatcagg gaatgccgtt atccttcgcg gtggcaaaga agcattcttc
    35041 tcgaatcaat tattagtcca attattgcag caagtactgg tcagtgctgg cgaatctcct
    35101 tatgccatcc agtttgtcga tgatacttct catgaaactg caggacagtt gatgcgattg
    35161 acagaatact tagatgtgct gattcctcga ggcggagcaa aattaatcca acgagtcaaa
    35221 gaacaggcaa cggtacctat cattgaaaca ggtacaggaa ataatcacgt atatatcgat
    35281 aaagaagcac agttatctat ggcagtgaac atcatcgtca atgcaaaagc ttcccgtcca
    35341 tctgtctgta acgcggcaga aacattgctg atccattctg agatcgcacc ttttttcttg
    35401 ccagctattg aaaaagaact ggtagaacac ggggtctctt tacgtgcgga tgctcgtgcg
    35461 ttagaatatc tagagacggc tgctttagca gaagacacag attgggatac tgaatatcta
    35521 gattatatcc ttgctgtcaa agtggtcgat tctttggatg aagcaattgc acatatcaat
    35581 cggcataata ctaaacattc agaaacgatc gtgacagata gttatgcggc tagtcaacgt
    35641 ttcttaaatg aagtggacgc ggcggctgtc tatgtcaatg cctccactcg ttttacagac
    35701 ggatcggtat ttggttttgg tgcagagatt ggtatctcta ctcaaaagtt gcatgcccgt
    35761 ggtccaatgg ggttaaatga attaacttcc acaaaatata tcatttatgg cgatggacaa
    35821 atccgcgaat aatgtttcgt gtgaaacatt attcccgcct tttgcctttt ttctgtaaag
    35881 gttctatcca actagaaagg tgtcaatatg tttgaaattt taatgatttt ttttatttac
    35941 cttatttctc tgaatatcgc tgcttttctg ggagtcggta ttttatccct tttctttcaa
    36001 ttcaaaaaac gaagcatagg gagtcaacgg gaaaaatggt cgcagtattt tgacaaaatc
    36061 gggcaaaaag ggctggttac acgcttgcac attagttaca tggttgcatt atgcttatta
    36121 gcgacaatca attactattc ttttttcgat cattcaatcg cttatacgat tacgctactg
    36181 attgctggga tttttcactt atcttataaa tatcagctga ataaaaacca cttgaatcgg
    36241 actttccgct aaaaaatatc cgtacgataa gtagagtgcc ttgaagatcg gcaaaaaact
    36301 gccggacttt ttagaggctg tgacaaagat tttgtcacag cctctttttc catataaacg
    36361 gcgttcgcct accagctgcc tggtaaatct gtttcctctt cgttaagaag cgaaaatccg
    36421 tcaaatgttt cgccatcata ataacgaagc agctcatcca tggacaagtt catcgttttt
    36481 tctttttctt cttccgctga ttcagccagt agctgttccc gatacacctg atacatacca
    36541 gctatttttt gctgcatcgc ttttctagtt tcaccttccg cattgattat tggcaattct
    36601 ttgatatagc cacgataaat caaccgatca tctgaaaatt caatgggtaa ttcaacgagt
    36661 gtcaacccga cttcctgaaa atattcttcc agcatctatt atccccccat tctttttctt
    36721 aaatgaattt tagcttacct actgaaaatt ttccagtttc attattttga gaaaaaaagg
    36781 gaatgtgaca aaaatcgtgt cacatcccct ttttccgaat aaatggtgtt cttctgccaa
    36841 ctcctcaatc ataagtcaca atattcaaca aacatgagaa actgtttttt gaaattgttc
    36901 cttgtgattc agagttaaac gacagtctca caacctcttg gtaaacggcg ttcaatcagc
    36961 tgaccctcgg tattagctca aaaaacggta aaatatgaga agctattttt ccgttttgtt
    37021 tctctaatac ttaggataaa acgctgattt tacaacctct gttaaacggt gttcaatcag
    37081 ctgtccttcg gtattagctc aaaaaacggc aaaatatgag gaactatttt tccgtttgtt
    37141 tctctaatac tcaggacaaa acgctgattt tacaacctcc tgataaacgg tgttcaaaag
    37201 cttgcatctg cggtaataag cccaaccaaa cgaaaaatat gagaagctat ttttgtttgg
    37261 ctgttcttat gacttgatgc aggacagctt tttcacaacc tctgatttct tattcttctt
    37321 catttgtagc aaaataatta cggtgataaa tgatacaaca tacactgata agaacaatgg
    37381 cacctgcaaa ataaaacgca tagctttcac caatatgttc accaatcaca cctgcaataa
    37441 atggtgctaa agctccaccg aaaaagcgga caaaactata gctggacgaa gcaacgtttc
    37501 gttgcagacc tggtatttcc atagcaatcg tcgtcaacag tgtattgata attccttgga
    37561 aaaatcctgc taacacaatg ctgatcggta caaggactgg catagaagtt ccagctccta
    37621 ataacagcaa acaaataatg aataagacaa atgctgctaa aattgtccga taagtcgtaa
    37681 acttccgctc aagagccggt gcaacgaaaa tcgaagcaat agccagaagc acaccccagc
    37741 caaaaaatac aaaaccgact tctaattcag aatagccaat caataaaaat ggcgcatacg
    37801 caagtaaagt aaagaatccg aaattatata acaatgcgat tagtccaact gaacgaagtt
    37861 tatgattgct taacgctttg acaccttccc aaaggcctac ttttctcttt ggtttctcag
    37921 tttcaggtaa aagtaccgct agggctataa aaccgcaaaa catcaaaaat gctaccccaa
    37981 aaaatggata acgccatgaa ccactgccca agaccccacc gactagaggg ccaacagcca
    38041 ttccgcagcc aagtgctgct tcatacatca tgattgcttt ttctgtgtta ccaactaaaa
    38101 tactgacgat cgttgctaaa gcagtagaga caaataaagc attcccgaca ccccagccgg
    38161 cacgtaaccc aatcagcatt ccgatattcc cagataatcc gccaagacca gcgaatacaa
    38221 cgatgataaa caatcctgtc atcaatgttt ttttcaaacc gatccgacta gataagaaac
    38281 ctgtaaatag catcaccaca ccagtgacta acatgtaact agtaaataac agggtcgttt
    38341 cagctggcgt agcgtttaac tgtgcagcaa tcgtttttaa gattgggtct actagaccca
    38401 ctcccataaa agcgatcata caggtagctg ctacaactag cgctgaactt ttcccctttg
    38461 tttcttcatt tgtttgttct tgtatatcta tttgattctc caatatttcc atccctttct
    38521 ctctattagc atcatacatg atttatttta catgtcaata tgcacatgta aaacgtggaa
    38581 actttactgt gatataataa agaaaataag acgagaggta ggatttttat gaatacttta
    38641 gcctatatat tattatgcgt gcttttatcc aaaccacgtt ctgggtatga attgaagcag
    38701 ctgatcacaa ttttttggga agcccatcac agtcagatct atacaacgct tgccaagtta
    38761 gaccagcagg gttatgtgac cgttttggac aaagacgagc actctcaaaa aaagatctac
    38821 cacttgactg atgaaggacg tgctcttgta gaggaatgga tcaaagagga aactcccgct
    38881 cctacgcaaa aagatgaatt tctagcaaaa atctatgctt tttcgacgct cgataaaaca
    38941 acagctactg gactgctatt cactagagaa caacagctga acaaaatcat gcaaaaaaat
    39001 caaaaaaaat tgaatgacct ctcgctagac aagaaacaag aatttggacg ttatgttgtg
    39061 atccagcgca gaattatgct ttgtcagcag gaactccaat ggtgtcagca agtaagagaa
    39121 caaatgcagc agttttttga gtaagggata aacagaacct ttctagaaat atctattgaa
    39181 gagcaaatta tcttcaataa aagagcaata cagttggaaa agaaaaagcg atccgaacga
    39241 caggccaaaa cctatcgttc ggatcactat ttttctagaa ctgcattcaa gagactatga
    39301 caaaaagcat gccaaggcct tttttcaaat aacagtgttc catcattcat tactttgaat
    39361 tgcttttagc acacttcttg aaagcgggcc aacgatgatc aactgcaacg gtaaagcggc
    39421 aatcacattc attttccaag cattcatata agcagagaat aattgatcag gtattccttg
    39481 ttccatgatc acaccgaata aagacataca tgtcaccatt cctaaaacca tcaagcatga
    39541 aatagttaag atcatttgga ccttgctttc tggatttact ggtagtttaa aggcaatttt
    39601 tttcgccagc acaccgatga tgaagacatc caaaatgaaa gccaccacga atccaggcac
    39661 tagaccaatc aacaaacttg ataaatgaag atcttgatgc agccataaat tatacgcgct
    39721 catccccaag accatcaaaa aacacatcat tgttgtaaaa attattcctt cttttttatt
    39781 tgtaggcaat ctgtaccact cctttaaatt tgtcacgaaa atcatcgtag cacatttaaa
    39841 aatttctcgt aagtactttt tttattttct gcccttttat ttcataaact tcatcagccg
    39901 tacgctcgac aaagtaagaa tcatgagaag taaacaaaat cgttcccgga tatatttgca
    39961 ttagcttctc taaagcttcg atcgttgcca tatcaataaa attagtcggt tcatctaaca
    40021 gcaacacatt acttggctta gtaaaaagca aagcaatcgt cagacgggtc gcttctcccc
    40081 cacttaatgt acataacggt ttcgtcaaag tttcctgagc aaaccctaag tgattcagca
    40141 aactacggac aagcgattct gaaaactctg tttgtctcat gacaaaacga atgatggatt
    40201 cttcagatgt catttgataa cccttttgct gatatacttg aaagcttact ttcggagaga
    40261 gaaggattcc ttctccttgt ttgcggatat gttctaataa ggttgtctta cctgaaccat
    40321 tttcaccgac aatcgcgatt tttttaccta aagaaaactg aaaatcacca tttaccagta
    40381 atgttctccc acttctttcc aattggacat tttgtcccat gatgggatag cggctgtgga
    40441 gttcaagaga ttttggtact ggaaaacgga tttgtttcat ctgctgcggc ttggttgttt
    40501 ctcctatttt ttggagtctt ctctctaatg ttttcgcatt cttttggatg gctttttgta
    40561 cactgtcttt ttgtttagag gaagacaacc gatccagtct gatttgttgc tttttctttt
    40621 tactggacac tttttccaac ttttccgctt gttttttctt ttcttggatc gatttttcca
    40681 atcgtttttt ctctttcatg atcgactctg cttctcgtag ctgagtcttt ttctccaatt
    40741 ccttttgagt gagataggca ctataattcc ccgaaaactc tcggatttct ccgtctttta
    40801 cttcccagat tttcgatgct agctcatcta gaaaatagcg atcatgacta acaaaaacca
    40861 gcgtaccata ataataacga agctcttcaa tcagtcgatc aatacttttt ctatctagat
    40921 gggttgttgg ctcgtctaga agcatccctt gttcataaga agaaagaaga cgtatcaact
    40981 cgatctttcg ttcttcccct ccgctccatt tccgctctga agtttcttcc agtcttaact
    41041 ggaaatgact ggctaattcc aaatttaggt cctttgcttc agcgagatag gttaattgag
    41101 gcaggtagtt gaaggtgatc tctgtttgga ttttcccatg atcgggaaaa agttcaccgg
    41161 ctattagttt gagcaaagtc gatttaccga caccattttc tcctatgatc cctatacgat
    41221 cttgctgata gacacgaaga gaatcaatag atagttctag ctggttgcca aagctgactt
    41281 gtagatttgt tatatttact gctaaatttt ccatatgttt tacactccta tttcatgtaa
    41341 aacaacaatt atcgatacta aaaaagagca gaaaaatctg tttgcaaaaa aactgcatca
    41401 catcttttcc tactctgaac gaaataacta tgactaaatc accaaatctg tcatagcttt
    41461 cacctattct actgtcgttc gaaaaaatgg acatactcca tccatgaaat tgttgtttat
    41521 acatgtcttt tgaatttatt tagttgttat tcaaaagttc gaaacgtaat ttcatcgatg
    41581 cagtcatttt tccgacctcc ttttttatta ggttaccaca cacgattcaa aaaaagcaat
    41641 agctactgaa tagtaactga aaacctttta ttcgaacata gtgatctttt ctggtgctaa
    41701 acgaacagac tcgtaacctt cttctaccgc ttttcctatt gataaaataa taactggaac
    41761 ataacggtct ttatccaaac caaatgcctc tgccaactga tctgcttcga atccaccaat
    41821 tggatttgtt tcatagccat gatcacgcgc aactaacatg aattgcatag ccgctaagct
    41881 agaatcgact ttgactacat cgttcatttc ttctcttgaa aaactcttgt aataaggaat
    41941 gatcgcagct aattgttgat ctctgacttc ttttggcatc ttccctgatt cgtaagcttg
    42001 gttgtagatt tcttcgccat attcgtaaca ttgcatatcg ccaaaaatca tgaccattgc
    42061 agaagaagta tcattttgtc tcgtattgaa acggatcaaa ggacgcaaga tttcttttgc
    42121 ttcctcgctt tcagcgacca caaagcgcca tggtttcata ttgacagaag aaggtgcagt
    42181 cgtcgcttct ttgatcattt ccagcatttc ttcgtgtggg attttgtatg tttcatcata
    42241 tacgcgaacg gatcttctac cagtcataat tgttgaaaaa tcattttttt gttccattat
    42301 tattaacctc tttcgtttct gttgttatat tgcaaagttg ggtattcaat ttatctaaaa
    42361 gtgttagaaa ttgtttcatt tctacatcgc tgaaagccgg ggatagcacg gaacctgtat
    42421 ctttatggtc catttcacaa tgcttgattt tcttctgggc tttttctgtt agcgctacat
    42481 acacttcccg gttgttctga ggatttctct gacgcgaaac atagccattt gcttccaata
    42541 ccttcagatg acgagtaact gctccttgat caatctgcaa cttttcttgc aaactgcttt
    42601 gtagcaatgg attttcttct ttcagtctca tcatgatctc gtatctagta agacttatac
    42661 cgatattttc ttcaaagatc tggacgattt tttgatcggc taatttgatt tgatataaaa
    42721 ggtgactaat ctcttctaaa gacataattt cctcctttct aataattgac tcgtcaacga
    42781 ttgacttgtc aataatagaa taaaaaaaga aaaaagtcaa agaatcagct gtgtaataaa
    42841 aaaaacgctc tcaatagaaa agtatagaga aaagaaattt tttgtagaat gaacggaaaa
    42901 aatgataaag aaagcacaac gctaaaacca agattattga ttcttatcaa actatagaag
    42961 atagctcttc tgcagtagat aattttacac cgccaaaaat ttccgaaatc gccttttgca
    43021 agaatcccgt cgaatattag aaattggatc agtatgctta aacagtaata aaaaagttat
    43081 gacaagatgt tgtcacaacc ttttttgagt aaacggtgtt caaaaacttg catctgcggt
    43141 aataagttca accaaacaaa aaacatgaaa atctgttttt gtttggctgt tcttactact
    43201 tgatgcaggg cagctttttc actacctctt tttaataacc ataaaatcct attccttcat
    43261 atctccagcc catcggtgtt agccaatctc tttccttagc actcaaggta tagtgatggg
    43321 taccagtttt ttcatttgga ttaaatagtc gataaattgg cttggaacca ccagaataaa
    43381 aacttagacc ttcataacgc caaccgattt ttgttaaaga gttgatctca gattgtgcca
    43441 ttgtgtaatg atggtctcca ctgtaaggat tgtataaacg gtagacaggt tgtccgctgt
    43501 ttggtgcttg ccaagcaatc ccttcatatt cccagcctac agttggcaga taattttttt
    43561 ctccggcatt caatgtatat aaatgttctc ctgtatgacg gttatacaga cgatacatcg
    43621 gagaagttga agttggtgct gtatcatact gtgtcagatt ataggtttcg attacattgt
    43681 tcaatgtaga accataatta ggcgcagtgg cataacgacc tgttagccaa gccgtagcat
    43741 ctttgtaaga gttcgtattg cttttccatg caccggaata gtagtaaacg cctggctgaa
    43801 aagaagtcgt cttcaatacc gttacgttat cttgcaatga ttctttgtac gaaggatatt
    43861 ttctaaaagc ggcattcacc gtaatccatt gcccattcac atattccgaa gtaggcatcg
    43921 taacagattg gccattatag cttcctttaa tcccaaatag attgtaatta ggggcagaag
    43981 ccaaagtact gttcccccag ccactttcaa cgatggcttg agcaatcatg actgatgcat
    44041 ataagtcatt ggcacttgcc aaactttgtg cagttgaggc aatactatca atgaacgctt
    44101 gttgagaatt agcagatgag cgagcataaa aagcatagcc agtatagttt tcgaattcag
    44161 acaaatctgc ttgatgggta tgtgtcaatg tttgcccatc tgtcaattgg ttcgtgattt
    44221 cttctgcatt ctctggattg ttctgagctt ctgctgatgt actgtcttcg gtttgtgttt
    44281 gttctgttgt ttcagttgtt ggttcttcgg ttgaaattgt attttctgag acagtagctg
    44341 actgcaaagt actttctgta tcgttttcaa ttcctgtttc tgaagaactc gatgtctgag
    44401 ttgcgacagt ttctgtcgat tcttcagcta gcactgttgt gttggcaact gctagatttc
    44461 ctaaaagaat aacgctaaat aataaattct ttgctttcat cttttttctc ctttgaatta
    44521 tgaaactttt cctaaataac agtgtaacta aaaaagtaaa agcaagtcaa tgtccatatt
    44581 taaataacat ttgtttcttt ttaataaatt tgctttttct gcataaaaag acgtaaatga
    44641 agaatattct tctccattca cgccttaaat tgatggtcgt ttgaataact atttaacaag
    44701 taattctctc tggatattat ccgttatgga ttatacttcc tgtgtcgtcg gacggataat
    44761 catgttgctg acagacatcc ggtctggcgt ttctatcgca aatgctacag cagaagctat
    44821 atcttcagat gttaatcccc attctttttg cgcttcatga agttcaagag cagccttttt
    44881 gtcgctgatg gtttgatata attcagtttg aacagctcct ggagagatga tcgtagattt
    44941 gatgttattt tgccgttgct cttgacgtaa tccttccatg attgcacgga cagcaaattt
    45001 tgttccgcaa taaacggcgg aatcaggata aacaacatgt cccgccacag aatcagttga
    45061 aatgatatgc ccagattttt gtttttccat gattggaagt acggcggaaa ttccatttaa
    45121 cacacccatg atgttgatat caagcatatt ttgccattca tcacggtgac cttcaactaa
    45181 tggagcggtt ggcatgattc ctgcattatt gtacaataca tcgattcgac cgaacttttc
    45241 catcgctaaa tcgataactg cttggacttg tgcaaaatct cttacatctg ctgtttgaat
    45301 atatagctca gcttctggta aagattcttt tattgctttt aggcgatctt ctcggcgtgc
    45361 agctataact agtttagccc ctttttctgc taatagcttc gttgttgctt caccgattcc
    45421 gcttgaagcg cccatgatga caatcacttt ttctgataaa tcattcattc tattctcctc
    45481 ctgttttgaa aaatctcttg cttatactca ctatctctta tttcatctta ctgttcaaat
    45541 aatccgttcg acaggtttga cgattttggc agggattcct gcaacgatgg tattgtctgg
    45601 gacatctttc gtaacagttg cattcgctgc aacaatggca ttctcgccaa tcgttacgcc
    45661 tggcagaata gttgctcctg ctccgatcca agcatttttc ttgatgtgga ttggctttac
    45721 tgacaagcct cttcgttctt ttggatcggt tagatggttc accgtgatga ggttgacacg
    45781 aggaccaatc aatactgaat cttctaaaca gatgccacct aagtcaacga acatcacttg
    45841 acgattgata aagacatttt tccctatact aatatgtttg ccgaaatctg tataaaaagg
    45901 cagcagaaca gtcaccgtat catctacttt gtcactaatg atattttcta gccgtttgcg
    45961 gatatctgct ggttcatggt acccagtatt cagcgctgct aaaggctctt cgttttccgc
    46021 ttggatctga tggatcactt caaagagcgg atccgttact cgaatctctt tattgacgat
    46081 ttgtttttcg atgaggcttg tcattttgct ccctcctttt atccttactt tataacggac
    46141 ggcttatgat gtctaatact tataaaacat acttaactat tccttttaac tatacttagt
    46201 ggtatgcttt tttaaaaaca ggagggaaaa aatggatatt cgtatactga cgtatttttt
    46261 agcagtagct agagaaaaaa atatcagccg agcagcaaaa tctctgcata tcacgcaacc
    46321 tactctctca aaacaactga aagatttaga agaagaactt ggtgtgacac ttttcactcg
    46381 aggctcccga gagatacagt tgacggattc tggtcgatat ctttattcaa aaggaaaaga
    46441 aatcttgtcg ttggtagaaa aaacggcgaa caatttaaca aatgacgcaa tcatctctgg
    46501 agaaatctac attggcggag gcgaaactaa agcaatctct ttcatttctg atagccttca
    46561 ttcgttgatc aaggcgcatc ctgatatcca gtttcattta tatagcggga atgcagatga
    46621 tatcatagac aaactggata gcggattact tgattttgga ctcgtaatcg aaccgacaaa
    46681 caaacaaaaa taccagtcat ttcgactgcc agcccaagat cgttggggac ttttgataca
    46741 tcggaatcat cctttagcaa agcaaacagt catccatccg caggaactga aaactgttcc
    46801 tttgatcatt tcccggcaaa cgtcagtgga cagtcaacta gctgaatggc taggtttcag
    46861 catagatcag ttatcgatcg tcggaacata taacttattg tacaatgctt ctttgatggt
    46921 cgataacggg acttttggtg cattagctct agatgggatc atcaatactt atgggacgga
    46981 aaaagtattc atcccactcg atccgccatt acaagcttca ctgaatctca tttggaaaaa
    47041 agaccagcct ctttctcctg ctgctcgtaa atttttagat gtcctaacgg agaaatacag
    47101 ttaagtagag tttttttcct tcgctttcag ctttttaaca acgaataagt taagcaaaag
    47161 atccaatgat taaataacat aagtttctta atttgttcct tccttttatt tctg
//
LOCUS       DACCXO010000011        44398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.011, whole genome shotgun sequence.
ACCESSION   DACCXO010000011 DACCXO010000000
VERSION     DACCXO010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.011"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            152..799
                     /locus_tag="IVY70_000556"
     CDS             152..799
                     /locus_tag="IVY70_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP7957716.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            814..2340
                     /locus_tag="IVY70_000557"
     CDS             814..2340
                     /locus_tag="IVY70_000557"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP7957717.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(2389..5187)
                     /gene="ileS"
                     /locus_tag="IVY70_000558"
     CDS             complement(2389..5187)
                     /gene="ileS"
                     /locus_tag="IVY70_000558"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP7957718.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(5449..6156)
                     /locus_tag="IVY70_000559"
     CDS             complement(5449..6156)
                     /locus_tag="IVY70_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP7957719.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            complement(6177..6959)
                     /locus_tag="IVY70_000560"
     CDS             complement(6177..6959)
                     /locus_tag="IVY70_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP7957720.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            complement(7008..7295)
                     /locus_tag="IVY70_000561"
     CDS             complement(7008..7295)
                     /locus_tag="IVY70_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP7957721.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            complement(7310..7909)
                     /locus_tag="IVY70_000562"
     CDS             complement(7310..7909)
                     /locus_tag="IVY70_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP7957722.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            complement(7923..8600)
                     /locus_tag="IVY70_000563"
     CDS             complement(7923..8600)
                     /locus_tag="IVY70_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP7957723.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            complement(8617..9858)
                     /gene="ftsZ"
                     /locus_tag="IVY70_000564"
     CDS             complement(8617..9858)
                     /gene="ftsZ"
                     /locus_tag="IVY70_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP7957724.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            complement(9881..11206)
                     /gene="ftsA"
                     /locus_tag="IVY70_000565"
     CDS             complement(9881..11206)
                     /gene="ftsA"
                     /locus_tag="IVY70_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP7957725.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            complement(11362..12582)
                     /locus_tag="IVY70_000566"
     CDS             complement(11362..12582)
                     /locus_tag="IVY70_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP7957726.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            complement(12597..13685)
                     /gene="murG"
                     /locus_tag="IVY70_000567"
     CDS             complement(12597..13685)
                     /gene="murG"
                     /locus_tag="IVY70_000567"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP7957727.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            complement(13711..15072)
                     /locus_tag="IVY70_000568"
     CDS             complement(13711..15072)
                     /locus_tag="IVY70_000568"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP7957728.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            complement(15086..16048)
                     /locus_tag="IVY70_000569"
     CDS             complement(15086..16048)
                     /locus_tag="IVY70_000569"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP7957729.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            complement(16074..18266)
                     /locus_tag="IVY70_000570"
     CDS             complement(16074..18266)
                     /locus_tag="IVY70_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP7957730.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            complement(18267..18668)
                     /gene="ftsL"
                     /locus_tag="IVY70_000571"
     CDS             complement(18267..18668)
                     /gene="ftsL"
                     /locus_tag="IVY70_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP7957731.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            complement(18673..19632)
                     /gene="rsmH"
                     /locus_tag="IVY70_000572"
     CDS             complement(18673..19632)
                     /gene="rsmH"
                     /locus_tag="IVY70_000572"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP7957732.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            complement(19653..20084)
                     /gene="mraZ"
                     /locus_tag="IVY70_000573"
     CDS             complement(19653..20084)
                     /gene="mraZ"
                     /locus_tag="IVY70_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP7957733.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            complement(20254..20622)
                     /locus_tag="IVY70_000574"
     CDS             complement(20254..20622)
                     /locus_tag="IVY70_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP7957734.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            20793..21749
                     /locus_tag="IVY70_000575"
     CDS             20793..21749
                     /locus_tag="IVY70_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7957735.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21822..21938
                     /locus_tag="IVY70_000576"
     CDS             21822..21938
                     /locus_tag="IVY70_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP7957736.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(21993..23675)
                     /gene="recN"
                     /locus_tag="IVY70_000577"
     CDS             complement(21993..23675)
                     /gene="recN"
                     /locus_tag="IVY70_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP7957737.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(23693..24142)
                     /locus_tag="IVY70_000578"
     CDS             complement(23693..24142)
                     /locus_tag="IVY70_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP7957738.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(24249..25067)
                     /locus_tag="IVY70_000579"
     CDS             complement(24249..25067)
                     /locus_tag="IVY70_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP7957739.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(25076..25966)
                     /locus_tag="IVY70_000580"
     CDS             complement(25076..25966)
                     /locus_tag="IVY70_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP7957740.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(25968..26192)
                     /locus_tag="IVY70_000581"
     CDS             complement(25968..26192)
                     /locus_tag="IVY70_000581"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP7957741.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(26197..27534)
                     /locus_tag="IVY70_000582"
     CDS             complement(26197..27534)
                     /locus_tag="IVY70_000582"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP7957742.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(27535..28389)
                     /locus_tag="IVY70_000583"
     CDS             complement(27535..28389)
                     /locus_tag="IVY70_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP7957743.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(28486..28935)
                     /gene="nusB"
                     /locus_tag="IVY70_000584"
     CDS             complement(28486..28935)
                     /gene="nusB"
                     /locus_tag="IVY70_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP7957744.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(28928..29353)
                     /locus_tag="IVY70_000585"
     CDS             complement(28928..29353)
                     /locus_tag="IVY70_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7957745.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(29599..30663)
                     /locus_tag="IVY70_000586"
     CDS             complement(29599..30663)
                     /locus_tag="IVY70_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP7957746.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(30835..31080)
                     /locus_tag="IVY70_000587"
     CDS             complement(30835..31080)
                     /locus_tag="IVY70_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7957747.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(31219..31593)
                     /locus_tag="IVY70_000588"
     CDS             complement(31219..31593)
                     /locus_tag="IVY70_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP7957748.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(31676..32590)
                     /locus_tag="IVY70_000589"
     CDS             complement(31676..32590)
                     /locus_tag="IVY70_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP7957749.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(32611..33414)
                     /locus_tag="IVY70_000590"
     CDS             complement(32611..33414)
                     /locus_tag="IVY70_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP7957750.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(33447..34436)
                     /gene="manX"
                     /locus_tag="IVY70_000591"
     CDS             complement(33447..34436)
                     /gene="manX"
                     /locus_tag="IVY70_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP7957751.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(34539..35030)
                     /locus_tag="IVY70_000592"
     CDS             complement(34539..35030)
                     /locus_tag="IVY70_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP7957752.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(35221..38055)
                     /locus_tag="IVY70_000593"
     CDS             complement(35221..38055)
                     /locus_tag="IVY70_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7957753.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            complement(38462..38755)
                     /gene="rpmA"
                     /locus_tag="IVY70_000594"
     CDS             complement(38462..38755)
                     /gene="rpmA"
                     /locus_tag="IVY70_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP7957754.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(38768..39112)
                     /locus_tag="IVY70_000595"
     CDS             complement(38768..39112)
                     /locus_tag="IVY70_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP7957755.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(39139..39447)
                     /gene="rplU"
                     /locus_tag="IVY70_000596"
     CDS             complement(39139..39447)
                     /gene="rplU"
                     /locus_tag="IVY70_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP7957756.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            39729..40370
                     /locus_tag="IVY70_000597"
     CDS             39729..40370
                     /locus_tag="IVY70_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP7957757.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(40432..42063)
                     /locus_tag="IVY70_000598"
     CDS             complement(40432..42063)
                     /locus_tag="IVY70_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP7957758.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(42186..42545)
                     /locus_tag="IVY70_000599"
     CDS             complement(42186..42545)
                     /locus_tag="IVY70_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP7957759.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            complement(42704..42777)
                     /locus_tag="IVY70_000600"
     tRNA            complement(42704..42777)
                     /locus_tag="IVY70_000600"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42741..42743),aa:Arg,seq:tct)
     gene            complement(43086..43769)
                     /locus_tag="IVY70_000601"
     CDS             complement(43086..43769)
                     /locus_tag="IVY70_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP7957760.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            complement(43897..44337)
                     /locus_tag="IVY70_000602"
     CDS             complement(43897..44337)
                     /locus_tag="IVY70_000602"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7957761.1"
                     /translation="MVPRIFGLTPYIIEDKEMSPYYAQGNLIYVRPKNTNEILVGDII
                     TYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGL
                     VLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKFL"
BASE COUNT        12581 a         9627 c         7509 g        14681 t
ORIGIN      
        1 aacgaaagaa tgagtcttct ctatcatctt tcgttttttg ttgtaaaaac gtctaaaaat
       61 cctttgtttt ttatttagaa aaatagtcaa ttaacgtttt ttttgttaca cttactaatg
      121 atggtttttt accatgaaag gatgatcgaa tatgacacca aatcgagaag attatttgaa
      181 aatcatttta gaactaggtg gagatacaac gaaagtcaac aataaacaaa tcgtatctag
      241 tctagatgtc tcagctgcat ctgtcagtga aatgatttct aaacttgtga aagaagagct
      301 tgtagaacat tccccttatc aaggtgtaca attgactgca agcggactgc aaaaagctag
      361 cagtctgatt cgcaaacatc gcttatggga agttttttta gttgaacatt tagactatag
      421 ctggaatgaa gtccatgatg atgctgaagt attggaacat gtgacttcgg aacatcttgc
      481 agatcattta gaaaactatt tgaatcatcc tgaacattgt cctcatggag gatcgattcc
      541 taaaaaagag gaagttgtcc atgaagaacg ccgacaaaca ttggagtcct atcctgtcgg
      601 tacaaaagtg cgtatcgcac gagtgcttga tgaaaaagaa ttgttggatt acttagtcac
      661 tattgacctg aatatcaacg aagaatatga gatcgttgac atcgcagctt atgaaggacc
      721 gatcactatc caaaatgata gaaagaaaat ccccgtcagc tttaaggcgg ccagcacgat
      781 ttttgtcgat aaaatataaa ggagtgtatc cacatgtcta atgaaaaaaa tgtattgttt
      841 acgatattcg gcggtacagg tgacctagcc cagcgtaaat tgtacccgtc tttgttccgc
      901 ctttacagaa aaggaaattt aggcgagcat tttgctgtaa tcggaaccgc tcgccgtccc
      961 tggagcgatg agcactaccg tgaagttgtc aaagaaacaa tccgtgcatt gaatccttca
     1021 gatgaagaag caactacctt tgcaagccat ttttactatc aatcccatga tgtaaatgat
     1081 tcaacgcatt atcagacatt aaaagaattg tctgaccgat tagatggaca ctatcaatta
     1141 aacggtaacc gtgtctatta tttggctatg gcacctcaat tttttggaac gatcgtttct
     1201 catttgaaat cacaaaatat tatgacaaaa aatggcttca atcgtttgat catagaaaaa
     1261 ccgttcggtt ctgattacca gtctgctttt gaattgaacc agcaaatcag agaagtattc
     1321 ccagaacaag atattttcag gattgatcat tatttaggaa aagaaatgat ccaaaacatc
     1381 tcagctatcc gttttgccaa taatattttt gaggctcaat ggaacaatcg ctatatcgac
     1441 aatatccaaa ttacttttgg cgaaaacctc ggtgtagaag atcgcggggg ctactacgat
     1501 catagtggcg cacttaaaga catggtgcaa aatcatattt tacaggttgt agcattactt
     1561 gcaatggaac cgccagtagc attttccgaa aaagaaatcc gtacagaaaa gatcaaagca
     1621 ttgaaagcta tccgcattta taacgaagaa gaagtgttag aaaacttcgt tcgcgggcaa
     1681 tatgctcaag gtgaattgga tggtgtacaa ttcaagggct atcgcgaaga agacaaagtg
     1741 gatgctcaat cagagactga aacattcgtc gcaggcaaat tcactatcga taacttccgt
     1801 tggtcaggtg ttcctttcta tgtacgtaca ggcaaacgtt tgactgaaaa agggactcgg
     1861 atcaacatcg tattcaaaca agttcctgtt aatgtattca aaacatctgt cgatgaacca
     1921 tgtgacgata cgacacttcc acctaatgtt ctaacgatct atattcagcc aactgaagga
     1981 ttttctttat ccttgaatgg gaaagaagta gggcaaggat ttgaaacgga accaatcaaa
     2041 ttggaattta gaaatagtgc agaaatggtt gaaaatagcc cagaagctta cgaaaaatta
     2101 ctattagatg cgttgaatgg tgatggaacg aacttctctc attgggaaga agtcgctcaa
     2161 tcttggcata tcgtggatgt cattcgaaaa gcttgggacc aaacacaacc ggattttccc
     2221 aactacaaag ctggatcaat gggacctaaa gcagctttcg aactacttga aaaagacgga
     2281 ttcgaatgga tctggcaacc agataactgg tatcgtgaac gtggaaaatt aaacgattga
     2341 taacaaaagg gggtgtgaca aagaacatgt cacaccccct tttccctttt acttttcttc
     2401 gaatccttca gctacagctt caggatagaa gtcttcaacg atttttgcac aacgaccgca
     2461 tagatgagga agtttttcat ctactcctac atctttacga acttggcgac agcgatcgca
     2521 agtttccccg tctgcttttt ccactgtaat agcaacatca tcaaatacca tagcgttatc
     2581 tggcacttgt tcatctgctg ccataacttc aaagtagtct ggtgaaacga tcaataattg
     2641 tgcaatatct gcatccaaag cttttaacat ttcacgaact tgttgttttg gataaacagt
     2701 tagttttgct tccaaagatt taccaatcaa tttgctgttt cttgcttctt ccaatgcttt
     2761 taagacatta tcacggaagt ccatgaaagc agtccaggta tccatcaatt cttcttggtt
     2821 tgcaaactct tcataaccag gaaactcgct taactgaacg tactcctctt cttctttcag
     2881 gaatgaccag atttcttctg ctgtatgcgg aatgatagga gtcaataatt ttgtcaaagc
     2941 tacagctgtt tgatagaaaa cagtttgcat gcaacgacgt tgataatcat cttctgcttc
     3001 aatataaact acatctttag caaaatccag gtagaatgac gatagatcaa ccgtcaagaa
     3061 gttcataact gtacggtaaa tatgcatgaa gttatatttg tcgtaccctt cttctcggat
     3121 ttctttgatt acttggttca atcgaacggt catatatttg tctacagaac gtaaatcatt
     3181 gtacgaaaca gtatcttttt taggatcgaa atcttctgta ttcgctaata agaaacgcat
     3241 cgtatttctg attttacgat atacttctgc cacttgactt aagatatcca tagaaacacg
     3301 aacatctgct tcataatcta cactagatac ccataagcga aggatatctg caccaaattg
     3361 tttgatcact ttttctggtg caatgatgtt tcctaaagat ttactcatct tacggccttc
     3421 accatctaaa gcaaatcctt gagaaataac tgatttgtat ggtgctacac cattgatagc
     3481 cacactagtc gtgatacttg agttgaacca gccacgatat tggtctgatc cttctaaata
     3541 catatctgct gggaatgata attctggtcg ctgacgtaaa acagcttcat gagaagaacc
     3601 agaatcaaac cagacatcca tgatatcttt ttcttttgta aattcgccat taggagaacc
     3661 tgggtgggta aatccttcag gaagaagttc tttcgcttct ttttcaaacc agataattga
     3721 tccatgttca gcaaataatt ttgccacatg ttcgattgtt tctggtgtga tgattgcttc
     3781 tccattttca gcatagaaaa tcggaagtgg tactccccat gcacgctgac gggaaattac
     3841 ccaatcgccg cggtcacgga tcatattata caaacgtgtt ttaccccatg ggataaccca
     3901 ctctactttt tctacttcat ccaaaatatt ctgacggaat ttatcgatag atgcaaacca
     3961 ttgtggagtt gcccgataga tcacaggctt tttcgtccgc caatcgtgag gataactatg
     4021 agtaaagaaa tctagcttca gcaaagctcc tttttctttt aatagatcag tgatcatagg
     4081 atttgctttg tcataaaaga taccttcaaa tccaggtgct tcttctgtaa acaatccttt
     4141 tgagtctaca ggtgacacta cagatagttt atatttgcta ccagcgatat agtcatcttc
     4201 cccatgtcct ggagcagtat ggactaatcc agtaccagcg tctaaagtaa catgatcgcc
     4261 taaaatcaca agtgattcac gttcatagaa tggatgactt gcagtcatat attccatttc
     4321 ttgtccagca aattctttta gaactgttac attttcccaa ccgatttctg attggactgt
     4381 ttctagcaaa tctttcgcta cgacaaactt acggccatcg gcttccacca atacatacgt
     4441 aaattttgga tttacagcaa taccttggtt tgctggcaat gtccatggtg ttgtcgtcca
     4501 aatgacaaaa gctgtatctt catcaagaat atctttgccg tctttgacat tgaaagctac
     4561 aaaaatagat ggagatttta catctttata ttcaatttca gcttccgcta aagaagactc
     4621 acttgacgga gaccagtaaa ttggtttcaa tcctttgtaa atatagcctt tttcagccat
     4681 tttaccaaat acgcgtactt gtgcagcttc ataagaagga tctaatgtaa cataaggatg
     4741 ttcccaatct cctgcaactc ctaggcgttt gaaatctgct cgttgcttat ctacttggga
     4801 aagtgcatat tcacgacatt tttccaagta ttctgccatg gacatttctt tacgtttcac
     4861 gcctttatta gccaatactt gttcaatcgg caatccatgt gtatcccatc ctggaacata
     4921 tggtgcacga tagccagaca ttgattttga acgaatgata atgtctttac tgattttatt
     4981 caatgaatgt cctaaatgga tattaccatt ggcatatgga ggtccatcat gaagaacaaa
     5041 gctaggtttt ccttcgttca actcttgtct tttttggtag attcgatttt cttcccattc
     5101 tttttgccac tcgccttctc ggtttggcaa attaccacgc atagggaatt ttgtttttcc
     5161 taattgcaag gtttctttca ttttcattca accatacact cctttatttg ttttccggac
     5221 acaaaaaagc atgttcagcc gcaagggacg aacatgccgt ggtaccaccc aaatttagtt
     5281 gcatctatct agtgcaaact ctctgtgacc aataacgagg tcagccgctg cttcttactc
     5341 taatttcgga aaagcaggtc tcaagtagag gatctttcac catgcagtgt cttaccgaac
     5401 ttccactctc atcggctcgc tttaagaata acaaagtgct tttctgttct actcgttttc
     5461 attttcacca ggcacgatcg agatatcaat agcctgtgta tcaaatgccg tcataggtgc
     5521 ttctatgact cctgtgtctg agtttacatc agtatcattt tcattgtcaa gctgttttgc
     5581 taaaacttct ttgatcactt catggctgtc agtcacgtag ctggagaatg gttgtaaaat
     5641 ttcatcccat tccggacttt tcacttgttc taattgagac tcaagcatca agctgatacg
     5701 ttgatggaat actcgtgttt ttttcttaag atcttcagtt tctgtcgcaa gctgtcttgc
     5761 tttttctgtt gcatctgtca agatttttct tgctttttct tctgcatcag ttgttaattg
     5821 atgtgcacgt ttttcagcgt tcgcaactaa ctcatctgct ttgttttgtg cagaagtaac
     5881 gatcacttcg gattccttgc ttgcacttgt ttttacttta tcagccgtat cttgagctac
     5941 gatgattgat tggttcaaag catcttttaa ttcattgaag tactcaagtt tttcttcaga
     6001 atgtttgact gctttttcta attctcgatt tctttgagtc aattcctcat agtcacgaac
     6061 aactaaatct aagaaatcat ctacttcgtc ttgattatat ccccgcattt ttgtcgggaa
     6121 tgtcttattt tggatatcta atggagttaa agccatgagt aaaccacctt tttattttat
     6181 ttacgtaata caccgaacag taccctgtac ttttcttttt tcgtcttccc ctcaagttct
     6241 tggatctgga ctctgccata acctctgact gaaataatat ctaatagttc aagtacaaaa
     6301 tcaggtcttg tattttctgt ccagttcact tttactttac cagcttcgat cagctgcttg
     6361 gaccgctgtc tggaaatatt ataaatctca gagatcacac tgtctagtcg catggaacta
     6421 actgttcctc tttcttgtgt ccagctatct ttcggcataa tcatttctgt gtatttgatt
     6481 ggttccaagc gtacagcaac ctttccaatc tttgttactt gtgcagcaac aaaatgatcc
     6541 acctcatttg caataaatac ttgccagcga tcaccgtccg agatgatatc gccaaagtaa
     6601 gagcgtttga tccccgttcc cactaaagtt ccaaggattt tcccatgact taggctggca
     6661 aatttgctag gataatggat ttcatacaat gctgtttcaa aatcttcctc tacaggttcg
     6721 taataatcgg ggaaaatcaa acatcttttt ctttctgcct gttcatatcc gccataaaac
     6781 atgaaacgca aatcactgct ttgtcgaatc agtgtttcta aaatatagga ttgtcttggg
     6841 tcaagaaatt cggtcagata gggtgcatat tgtccttgaa cctgttcaat ccaatcgcct
     6901 accgaatcga taaatgtacg ttcatctgcc cgaaagtgct ggtatacatt tgcatccaaa
     6961 attttcccct cctatctatt ctgagaataa agattttgtt gcttcaatca ataaactaaa
     7021 tggacagtta tccatataac gatctgtgct aaaccttgta cggctaagtt caagatcagg
     7081 attgccccta aaattgtgaa atccaagggt ccaatcctca aaggcagacg atcgaacaaa
     7141 ctgatatacg gttcgcagat cttagctaat aatcgtccta gccctgattg atacccgcct
     7201 ggaaaccaag agagcaatgc atataccatc aagataatgc tatagaaata ggctgcccgt
     7261 tgaagtaaat gaagtaatag tgcaatagta taaatcgtag ttcctcctct tacagatcaa
     7321 acaaattcgc atctgcaaaa gattgagcgg ttgaactgtc aatctccatc ccagtcggtg
     7381 tacaaagaaa aatctcatct ccaacacgtt tgatatctcc atctagtgca taaactgttc
     7441 ctgttagaaa atcaacgatt cttctcgctt gtttttcttc tactaaatga aaattcacta
     7501 aggttgcttc tccagcaatg attcgtttag caatcgccat agcttcagaa taagctcttg
     7561 gttccattac agtgattttt ccagcaagat ttgctttagg ttgtgtagag gttcttgaag
     7621 ctgttctttc tttcgctaca gtttttgtag gtgattgatg tagttcaatt acttttttct
     7681 tttcagccgg ctgaatactt tcatgcgtta caggacgtga agtcgccgtt ttttgttttt
     7741 catgagtatt cgcagcagat acagaatgag ttgattgatt ctgtgttgta ttttttttag
     7801 ttggctgtac agcctttttt gttgtttcag gataatcttg gtattcgtac tcttcctcat
     7861 cagctaatcc gaaaaaatta gcaattgctc ctctgtttaa aagcggcatt tttgttcctc
     7921 ctttattcgt ctttgaatag cgctgttcct acacggataa atgttgcacc ttcttcaatc
     7981 gcaatttgaa agtcttgact catccccata cttaattcac tacatggtgc atgggcataa
     8041 ccttttgctt gaacaagatc acgcaattct ttcagcgtac caaacacttt atggatttgc
     8101 tcatcagaag cttctttagg cgccatagtc atcaacccaa caaccttgat ttttgtgcag
     8161 gcagctaaag attccacaaa cggtaaaagc tcatctggtg aaattccatg ttttgattcc
     8221 tcaccagaaa catttacttc aataaaacag gataattctt tctctaaccg cttctggatt
     8281 tcttctgcta atcttaaact gtccaacgca tgaaagtaat caatttcatt tacaatcaat
     8341 tttacctttc tgcgctgtaa attaccaatt aaatgccact ttatttcagg aaaatcaaat
     8401 aaatcttgtt tcttttctaa taatttatct actcggtttt ctgcacaatt cataacgcca
     8461 gctctaacca attcttcagc ttgagcggat tcaacagatt ttgttacagc tactaatgta
     8521 acttctttcg gatcacgatg aacaagagca cacgaatcct gaatttcttg cttgattttc
     8581 tgcaagttgt cagcaatcat gtgctcttcc tctcctttat tttttacgac ggaaaaacgg
     8641 tggcgtattt aattcatcat cgccatgaga ttttgtttct tcgcggttga atgtatcaaa
     8701 ttcttttttt tcgatattgt caaaattgga atcatcaact ctaggacgaa cattttgttc
     8761 acgtctgata tcccaatcgc caaaactgtt ttcttcttct gaagaagttg gtttagcttg
     8821 atccatatct aatacagtct tttgagcagg tgattgcatt tgtgcttgtc tagctggtcg
     8881 gctagacttg cgttcctttt ttgattcatc gatacctgtt gcgatgaccg tcacacggat
     8941 ttcatcgccc atttcttcat tgatagatgt acctagaatg atatttacat cgccagttgc
     9001 tgcatttgct acgatatctg aagcatcttg tgcttcgaac aatgtcatat ccaaaccgcc
     9061 tgtgatgtta agaagtactt gttcagctcc atcaatagat gtttcaagca atggagagga
     9121 gattgctttt ttcgttgctt ctactacgcg atcttctcca ctagccacac cgatccccat
     9181 caatgctgta ccttggtttt ccatcactgt tttcacgtca gcaaagtcaa ggttaacgta
     9241 acctggcgct gtgatcaaat cagagatacc ttgtacacct tgtcgtaaaa cattatctgc
     9301 ttcacggaaa gcttcaagca tcggtgtctt tttatctaca acttctaata aacggttatt
     9361 tgagataatt aataatgtat ccacattttc tttcaaacga gcgatacctt ctgcagcaaa
     9421 acggccacgt ttcggtcctt caaaagtgaa tggacgtgtc acgacaccaa cagtcaatgc
     9481 gcccaattct ttcgcgatac ctgcaacgat tggtgcagca cctgtaccgg ttcctccacc
     9541 cattcctgca gtgatgaaaa tcatatctgc accgtctaaa gcttcgcgta aggattgttc
     9601 actttcttct gccgcttttt gtccaacttc tggctgagaa ccagcaccta aaccacgagt
     9661 atatttgggg cctaattgga taactgtttc agcttttgaa ttttttaatg cttgcacgtc
     9721 tgtgttggct gtaatgaatt caacaccttt aacgttttct tcaatcattc ggtttacagc
     9781 atttccaccg ccaccaccga ctccgatgac tttgattact gcgccgtcat tgatattatt
     9841 atcaattgag aattccatat cttattcctc ccaggatgaa ttaatcaaaa atatttgtaa
     9901 agaaaccctt gattttattt tttacatttt ctccggactc ttttggttcc ggctcatcgt
     9961 agattgtttc ttcaggtgct tcatagctct catatgtgtt tgttgtttct tgttcaacta
    10021 ccatttgacg agctgctgag gtttcacccg taacagcaat ttttgccaat tgatacactt
    10081 cactcaagtt tgctgaataa tccacgatac ttattacatt tgtaaatacc gggtttctca
    10141 gccccatgtg atttggaaca tacaatttta catttacgcc gaagatttct tgtgccaagt
    10201 ccacaacacc aggaagacta gcagctccgc ctgtcaatac gacgccacct ggcaactcta
    10261 atgcttcgat ttggtccaat gcatcttttg ctttgttgaa aatctgttcc attcgagctg
    10321 aaataatttc tgacaagtag cgttcatcga ctttcactgg ttctgactga ccaatcacat
    10381 ctactggaaa ttcttcatct ggtgatgtcc gttctgggta tgcatccccg tagtttattt
    10441 tcaacgcttc tgcattgtta aatgacgtat ttaatacaat agagatatct ttagtgacaa
    10501 attcgccacc ttcttggtca agattggtaa acttcaattg cttatcatgc ataacagccg
    10561 ttgttgtttg gccgccaccc atatcaatga cgatcgtacc aaaatctttt tctccgtcag
    10621 aaagaattga ttcagtcagc gctaaaggag taatcaccat ttcgttgacg attaatccgg
    10681 cattttccac gcatttacgt atgttatgga tgatcgtttt aggtcctgta aacaacagac
    10741 cgtacatttc taatcttacg ccgatcattc cacgaggatc tttgattccc tcgaacccat
    10801 cgacagtaaa atcttgtggc aatattgaca cgatttgacg ttcaggaggt attgaacgaa
    10861 caagggcagc tgaagccaca tttctgacat cttcatcggt gatttccttt gaatctccgt
    10921 taactgcaat cattccctgg caattttcta cttcaagcat gtttgctggc aaaccgacac
    10981 ttacgccttt gatctggata cctgcttttt cttctgcttg tcgtactgct cgttgtatcg
    11041 cttggaccgt tttatcgata tcaacgataa tgcctcggtt aatcccttct gattttgcgt
    11101 ttcctactcc aataatattc atttggctgt cgatatattc agccacgaca actttaacag
    11161 atgtcgttcc aatatcaagg cctacataca ttcctgtttt tgccatgaat gggattccct
    11221 cctacttact attccatata caagcgaaca actagcgtcc gttttaaaat atacttatta
    11281 atagatatat tcactgcttc caattctacc ataaattact ggtttttaga aagtacattc
    11341 ttacttttta aagttgtttt atcaattttc atttaaactt gacggagttg tacttggatt
    11401 atcttctatt tgacttgttt cacttgaatc atttgaatca gatgatccgt ttgtgctaga
    11461 cgatccaggt gtttgtgctt tttgcgtttc tgcctcttca cttgattcgt ttgtttgatc
    11521 cgcattttct tcactttcag ttgtttcttc cgattggccc gattcttccg tttcttgagt
    11581 gctaccgtaa ggatgcgaga agataccaac ttccatgtcg atcacacctt tttcatccat
    11641 atctttggct acttgcgaat aatatttcat ttggttcgca agattattga tatttacaat
    11701 aacttgattg ccgtcattca tattcaattg gatcagattt ttattgcttg ctcttggcgt
    11761 gtacttgatt tcagaaattg ctttttgtaa ttctgatgat aactgattgt attgctttgt
    11821 taattctttg atttttgcag agtctttgaa ctcttccaag atagggagat tttttgtagg
    11881 atttgctact ttttcatcta atactgttcc attttcaatc actgggtagt attgattgtt
    11941 ttttgcgatc aaagcaatct ctttatattc tgttacatcc aaatcgaatt cattcatccc
    12001 tttgaaatgt atatttgctg tttcgattct tggctgtgct tttttaaggc gatcgatata
    12061 agtagatcga tgaaaatact gtccccaaac attttctcct aatgacaatt ttgtatcaga
    12121 gatgatttct tggctgttga ctgtcttatt gcctgatact gcaactgctt gtaatttgct
    12181 ataaggagaa acataataga tcaaaaaaat cagcggtata cccaatacag tgatgatcaa
    12241 agctaaccga cgaaatagta ctttgtgccg ttgacttttt aaattgggca gccggttcaa
    12301 aaaagaaccg ttatatggtc caccgttttt cttttgcttg atctcttcaa aaaattcgat
    12361 ttttttctcg gactggcttt cttcttgctg ttcctcttga accgtttttt cttcttcaga
    12421 atctgaaagg ggtgtatcta atgttatgtc ttcttttttt ggctcttggt ttttcatctc
    12481 ttgttctgcc agatattttc ggttagcctt ttgccaagga gtcaaattgt tttcatcaga
    12541 agacggatga gacaattttt ttggttcttt tttcttactc actaagacca ccgcctttat
    12601 tttttgatta tttgttttac taagtcataa agacgttttg aagcatcagg gattccttgt
    12661 tctttagaag ctcggcacat ttgcttttgc aattcttcat cacccatgat ttcgttgact
    12721 gtctgactta gtgattcacc tgttagttca ttatctgcaa tcatttttgc tgctcctgcg
    12781 tgaacaagac tcattgcatt tttagtctga tgatcattcg taacatatgg gctgggaacc
    12841 aacactgccg gtaaccccag agctgtgaat tctgcgatcg aagttgcacc agcacgcccc
    12901 accagcagat cactggaagc catgacctct gccatatttt tgatataagg ctggacacta
    12961 atattttgta tttcaggatt tattttattt aggatctctt cataatatct atctcctgaa
    13021 gcatacaaaa cttggtaaga agaaggaata tattcactta aaaaaactga tactgcttga
    13081 ttgattttaa gtgctccttg actaccgcca aaaatcaaga ctgttttctt ttctggatcc
    13141 aaatcaaatg ttttcaaaat atctgatttt ttagtatctg ctacttcttg tgctcgagga
    13201 ttacctacaa gaactgcttt gttttcagga aaaaaggaag ccgcatcttt aaatgaaaga
    13261 gcgatccgat ccacatatcg gcttaaaaat ttattggtaa cacctggtac actgttttgt
    13321 tcatggataa ctgttggaat acccattttg gctgcagcgt acactaccgc tccggaaaca
    13381 tatcctccag taccgataac gatatctggt tgaaaatcac tgaggatttt ttttgcttga
    13441 cggatactct taagaaacaa tcggaccgtc ttgatgttat gcatggaaat ttttcgttta
    13501 aatccttgaa tctccaaggt atggaaaggc attcctgtat cagggacgat tttgttttct
    13561 agacctcgtt ttgctcccac atacatgaat tcagcattcg gttcttgagt ctttacataa
    13621 ttgacaaagg caagagctgg ataaatatgt ccccctgttc ctccgcctgt aaccaaaatt
    13681 ttcatttttt atcgctcgct tctacttttt ctattttaat cggttcacag cttccatgaa
    13741 tctgtttcct ctagtttcaa aatttgggta ttgatcccag ctggcattcg ctggtgataa
    13801 taaaactaca tcaccatctg tactgttttc taatgctaaa ggaactgctt gttctacaga
    13861 atcggctgtt actattgtct cgatgcctgc tttttttcca gcatccatca acttttcttt
    13921 tgtttggcca aatacgacca ttgctttgat tccttttaat gaaggaatta attcatcaaa
    13981 tgtatttcct ctgtctaatc cgccagccaa taacacgacc ttagacgttt caaatccact
    14041 tagagccatt ttagtcgcta aaatattggt tgcttttgaa tcattataaa attttctgcc
    14101 ttggatttct cctacatatt gcgtacgatg tggtacgcca tggaaaaaat gcaacgtttc
    14161 tcgtatagct tcatttgata taccatacaa ttttgctaca gaaatagcag ctagtgcatt
    14221 ttctacatta tgcttacctg gtacgccaag ttcagatatt tccattattt tttctttttt
    14281 atagtatatt gagtaatcgt cagcatacac accgtcttcc aacacttctt ttgtcgagaa
    14341 aggcagtacg cgtgcttttg ttctttgcgc taattcttgt agttctgact gattccagtt
    14401 aaggatcaga taatcttttt ctgtcatgtt tttttgaagg ttccatttag ctttgacata
    14461 ttcttttctt gtaccatgat aatcaatatg tgcttcatag atattggtaa tcaccgctat
    14521 atgagggcgg aaatctgtga ttcccatgag ttggaagcta gacaattcca tcacaatttt
    14581 atcttctgat tttgcttctt gcgctacacc acttgctgga tacccaatat tacccgctaa
    14641 acgtgcaatt ccttgatctg ctcctgcatt cagtagtagt cctgtcatcg ttgttgtcgt
    14701 tgtttttccg ttcgttcctg taatcccaat aattgggcat tccgctactt cgtaagctag
    14761 ctcaacttcc gtaattaccg gaatacccat ttcttgggct tttgctacta aaggatgact
    14821 gtagggaatc cctggatttt tgaccatcaa tgaaaattct tcatccaaca attcgattgg
    14881 atgactacct gttattactt ttatccctaa agaaagcagc tcttgtgctt ccggattttc
    14941 gtcaaaaggt tttccatcat taacggtgac aagtgcgccc aattcatgta ataatttggc
    15001 cgcacttact ccgctttttg cgagtcctaa gacaagcact ttcttatttt catatgttga
    15061 tatttttttc atttcccgtc atctcctacc aaacgatcca taatgtgatc aacgaagtca
    15121 caatgctgat caaccaaaaa actgtatcaa tcttccactc cgaccatccg cacatttcaa
    15181 agtgatgatg aattggagac attttaaaaa ttcgttttcc tgtaagctta aaggaagtaa
    15241 cttgaagcat cacacttgct gtttccataa catagatcaa accaatcaaa agcaatgtcc
    15301 attcttggtt tagcattata gaaattgccg ctaaaagtcc gcccaacgct aatgaaccga
    15361 catctcccat aaaaatcttg gcaggttttc tgttgtagac gaagaaaccc agtaaacctc
    15421 ctaaaacact taagcaaatg attaatacat catactgatg ctgatgccaa gcaattagac
    15481 cgtaagctgc aaaagagata cttcctaagc cagcaactaa tccatcgatg ccatctgtca
    15541 gatttactgc atttgaaaat ccaacaagcc agaagagagc gaaaatacca taaatgattc
    15601 ctaaaggtag atctagtccg aagaaattca atacacctgg ataaccttca gaacggtaaa
    15661 caagataaaa gatgattccc cctagaatct gtccaatcaa tttctgcttc gagttcagtc
    15721 ccatgtttct tttcttgaat atttttataa aatcatcaag aaagccgatt actccgtata
    15781 acgctaatac aaaaagcaag atgaaaagag taggagttag ctgtttttgc caagctccaa
    15841 cccaaattcc tgtcaaaata gagccgatca gaaacactaa gccgcccatt gtaggagttc
    15901 cggctttgct attgtgccac tttggtccct cctcacgaat cgcctgacct tgctttttca
    15961 tttgaaaata accaataaat agcggcattg cggcaattgt cattgcacaa ctgctgacaa
    16021 tgggaatcaa tgcttgtgtc cattccatgc tcttttctct cctatttctc ttcttattct
    16081 tctaaagtaa aactaagttt gtcttcagtg attttttcat atggagcaag ttcttgtttg
    16141 acacaataac catctccatc aaaactaact tcaataccga ggattttccc cagcttcatg
    16201 atatctgctt ttgaccatcc tgttacgtca ggcatcagct tatcaccacc agtatatagg
    16261 atcagttttt ctccagagat caactgatca ccgttggcag tagattgttt ttgtactttt
    16321 ttaccattac cgatcactac aggttgcagt ccactttttt gagcatcagc tgctgcaacg
    16381 tccgcttcaa gattacgata atcggctacg gatacttttt ctgtttttgt ttctgtatct
    16441 gaatcttctt cagtttcttt aaaatccatt gcccgtttca tcaatggatt tgcgatttta
    16501 gccaatgcca ttcgatcata tgtcttaggg tgtttcattg ttagatacaa gacatagtct
    16561 ggatcttcag aaggaaccat ctccacaatc gaatacaggt aggctgtatc tcctgtttga
    16621 tatccccctg tatcagaagc gatttgggct gttccgtttt ttgccgatac attgtatcca
    16681 ggtacactat atacaccata agcactaccg tagttttcgc tttcgacaac atctctcata
    16741 tattcacgga ctttttctgt tgtttctttt gaaaaaggtt gccctagaac ttctgtttga
    16801 gttgttcgct cttctccagt ttggggatcc accactttgc tgatatatcg aggttgaatc
    16861 attgtaccgt tattcgcaat agaagtgaaa gctttcatca tttggaaatt cgtcacacct
    16921 accgcttgtc cataagcact cattgctcgg tcgacaatat ttaatgtagg taaagcaccg
    16981 tttacttcat catctaatcc agaatgagta ctttgtccaa atcccagttt ttgtaaatag
    17041 ttataccatc gtccgcctaa tcgctgttcc agtatgacca tcccgacgtt acttgaccat
    17101 gaaagagctt gacgcatggt cagcacacct ttttcaccaa agtcatggtc attgatcgta
    17161 gcatcttcta cttggatttt tccagattgg aaggtttcat tctcgttgaa ttctccttct
    17221 tcgatagcag ctgcagtagt gaaaactttc atggttgatc ctggttcata actatcttgt
    17281 accaagaaat tacgccagat agcatctttt ccagtcaatc cttccattgt ttcaggattg
    17341 aaagtaggtc gttgtcccat tgccaagatt tctccggttt ttgctttcat caggactgct
    17401 gtcaattctt ctggctgata ttcttcattt acttgatcca tcagtgtttc taaataactt
    17461 tgtagtctgc tgtctaaagt cgtgtagata tcttgtccgt cgacagcttt ttcttcttct
    17521 gccactgtac ctggcagagg attttgaaaa ttatcttttt gataaataat cttaccatct
    17581 ttaccgctca agatatcatt gtaagcagac tccaaaccta gtttaccaac taagccattc
    17641 tcatctttgt ctggaactgc gtatccaata aaatgagaag aaaagacgcc atttggatac
    17701 atccgagcct gatggtcgac aaaataaaga ccagcgacac cttctttttt catgtccgct
    17761 tcgatgtttt gcttggtttc ttgggagata tttttcccat agctaccaaa ttcaacttga
    17821 tacagctctt tttttgcgcc gtcctctaaa atattcagtg catctttttt gtctatagaa
    17881 agattcttat gcaggatttc tgctatttta tcaaaattct tttcttcaac atacagtttt
    17941 ttatccccag aggtatatgt ttttgagagt atcgctttaa ttgaataaga ggaggcatct
    18001 tctgcgagcg ccacaccgtt acgatcataa atcgttcctc tttttgcttt tactacttca
    18061 cttccttggt acaactgttt tgttttttca gctaatgagg taccagcaac atgacctcca
    18121 ataacgatat acgagaaacg gacggcaaat aaaaagaaca atccaatact cgtagcaaat
    18181 aaaatgatac ctactttttt gcgattgttc atcggattta gatttttctt ttccataaac
    18241 cgccgaattt tatttttaag actcatttac ttcactttcc ttaaattgtc gtcattaata
    18301 gacaatcctt tttcttctgc gatttgttta atccgttcag tacgagaaag ttcgtttttt
    18361 tcttgttcta aactcgtttt ttcttgcttt ttatcattga tttctgtttg tattgacgtg
    18421 atttcttttt cagctccgct gatatttgtg cgcagcataa ctgttaaaac acataaagca
    18481 acaatagcaa tcgccacgct tgccacagca attttttcca aagcagaaat ctgctttaat
    18541 cgttttgccg gtgattgtgg aagaatgatt tcttgattcg aagaagaatc aggcatttgt
    18601 tgttccggct gatccaccaa ttcaggtaaa tcatattcgt aatcttgcat tttctttaat
    18661 tctgccatca ttctactcct ctttcattct tattttttct gcaatacgta actttgcact
    18721 gcgtgatcga ttgttttctg ttagctcttc ctctgaagga aggattggtt tacgagtaat
    18781 gactttcagt tctggttgga attcgtcagg tatgacagga agtcccggtg gtagatcttg
    18841 taccgtacta tattctttaa acatactttt gacaattctg tcttccaatg aatggaatgt
    18901 gattacgctg attcttccat tgactttcaa taaccggatt gcctgttcta aagaggcctc
    18961 ttcggcaccg agttcgtcat ttactgcaat ccgcacagct tgaaaaattc tttttgcagg
    19021 atgcccgcct tttcttcttg cgggagctgg gatgacttgt ttaatgattt cgacaagctc
    19081 accagttgtt tggattggtt ttgtttttcg tactcgttcg atttcacgag caatttgttt
    19141 ggaaaatttc tcctcgccat agcggaagaa aatctttact aattcatggt aggaatattc
    19201 attaattacg tgataagcgg ataaaggagc tgactgatcc atgcgcatat ccaacggtgc
    19261 atcttgatga taactaaatc cacgttctgc ttcatctagc tgaggagagg aaacccctag
    19321 atcatacaaa atgccgtcta ccttatctac atgctcactc aagcgttctt ctaattctct
    19381 gaagttcgct ttgataaatg tgaccatacc ttgttcgaca taagaagcta aacgcagctt
    19441 ggcgtgatcg attgcttttt gatcttggtc aaacgcatac agatgccctt tagaacttag
    19501 ttggcttaat aaatattcac tatgtccggc accgccgagt gtacagtcta cataaacgcc
    19561 atccggtttg atgttcaatc catctaccgt ttctttcaac atgactgtat agtgttgaaa
    19621 tgtttcagtc attagtcagc ctcttcctat tcttatagtc cgaaatcaat catactttca
    19681 gcaatttcgt caaagttctc ttctgctgct gcagaaaaat cttgccatcg tgtctcatcc
    19741 caaatttcaa ttcggttaga tacaccgatg actacgcaac cttttgtcaa agctgcataa
    19801 tttcttaacg tgctgggtat attgattcgt ccttgtttat caagttcgca ttcagtggca
    19861 gccgaataga agaatcgcac aaaagtacgt gcgtcttttt tagccaacgg catttcgttc
    19921 agtttagctt ccagattttc ccattcgctc atcggatagc cgaaaagaca gccatccaat
    19981 ccgcgtgtca gaacaaactt ttcgccaagt tcttcacgaa gttttgaggg cacgattaaa
    20041 cgtccttttg catcaatatt atgttggaat tcacccatca acattgaaag ccccccattc
    20101 ctccactaac taaaattagt ctaccacaat cccccacttt ctaccacctt ttctgggaaa
    20161 tccgggaaca acttttttta ttcataaaca tttttttaaa aatcaagctg caaaaagcct
    20221 tttaaaagac tttttacagc ttttctattt ttttcatagg taagaaagta tgttcacaat
    20281 gatcaagaca gcgtacaaga cgattgtcaa cagaaaaacc aaacgccaag tcatcttgaa
    20341 ataacgcgga taaataattt cttcataata atatgcttgg aaaacagcaa tacaaattcc
    20401 cattagcaaa acagaaataa agaaaaacgg taagatagaa attgtccctg tatttctaga
    20461 caatgcatgc ataccgcccc acaaaaaagg aatcgccaaa tcaggggctt ttattttaaa
    20521 acgacgattt aacgaaaata attttacaag aaattggcaa ccaaacaaaa cgattaccgg
    20581 gtaaatatac caaaacaaga taagtggtgt gaatgtgccc atgaacgtaa actccttttt
    20641 tatcgttgct tcaagtatac tgtatatact ttacacaact gtttcatttt atttaaattt
    20701 atgcagttat attctatatc ttacaaacac attccatgta ttccaattcc ctatccgtta
    20761 taataaaaga aacaggcaac aggaggaaaa tcatgatcaa atattttaca ttagataatg
    20821 agaaactgcg taataccgga aaggaaactg accaaacgat ctggtattgt gtagaacgtc
    20881 ctaatcaaga agagattcat cagctgacac atcaatttga aattccgtca gattacatca
    20941 cttccatatt ggacgatgca gaaaattccc gtacagaaga attcaatcaa tcaaagttga
    21001 caaagccagc gttattattg ttacaatatc cttttgctaa aacaagtcct agcggttatt
    21061 ttcaagtgga tacatacccg ctctctatta ttttgacgcc caaaaagaag cttatcacaa
    21121 ttacgaatca tgaaccactg tttttaaaga aagtctttag ccagaccttt ccaaaaagtg
    21181 atatttcgcc taatctcaat atcttctttc aattcatttg gcaggtcaca ctctcttaca
    21241 acagttattt agctacgctt caatctcaat gcgatacttt ggaagccaaa cttcaagtat
    21301 ccactgaaaa caaacaactt tatcaaatca tggatatcca aaaaagcctc gtttatttca
    21361 gagaagcgac ttctgctaat ttagatgcat tgaaagaact agcgcaaaat aaacttttcc
    21421 aagaaaatca tgctttgaaa aatcatttac gtgatgtatt ggtggaaaca gagcaggcaa
    21481 tgactacttc tcgcattcaa ttaaaactag tagaacaaat gaatcagact ttttcagcaa
    21541 tcgtatcgaa taatctgaat aatattatga aaattttaac ttctttaacg attattttaa
    21601 ctataccgac aatcgtcggc agtatctatg gaatgaacat taagttacct attgctgaaa
    21661 gggatgatgc ctttatatgg ctagttctat taacattatt tatttgtatc gtaactacct
    21721 attatttaaa aaaagggaag tttttataaa aaagccctct tgtggaaatg gaaaaaagat
    21781 tgtacacttg aatcaactaa ttaatgacga ggttgaaaac aatgcgtgaa aaaaaacaaa
    21841 caagtacttt tgctaaagta acaaaatttg tcatttggac aatgctgatt ttaacgattg
    21901 gatctgttgt attgactgct gtaatgagtg tcatgtaaaa ataaaatata gctatcagct
    21961 gctgaaaagg acctgaacca ctgcgaggtg gctcaggtcc tttttgcgag tttcaatagt
    22021 tcttttgcat gttcgagtgt gagttttgta atctcacttc cagccagcat acgtgcaatt
    22081 tcttcttctc tttcttttgt tttcaactct gaaacagaag tttgcgtacg tccatcaatc
    22141 actgctttca caatataata ttgatagtcg gcgactgcag caacttgagg cagatgagtg
    22201 atgcacaaaa cttgagagta tttcgatatt tgagaaattt tatctgcgat ggcctgtgca
    22261 actcttccgc tcacacctgt atccacttca tcaaaaataa tacttgtcac accttgctca
    22321 gaagaaaaaa tagttttcaa agcaagcaac atccgagaca gttcgccacc agaagcgact
    22381 ttcaccaaag gcttcagtgg ctctcctgga ttggtcgtaa tgtaaaattc gatttggtca
    22441 aatccatcat gattcaattg tctattttct tcttttaaga aacgtacctc aaactcagtg
    22501 ttttccatat acagactttt caattcatgc agaatggatt tttccaattc ttttgcgatt
    22561 tttttacgcg cttcatgaag atctgcggct tgttgctgaa gtagaagctc tttttgctct
    22621 agttcctctt tcattttgtc caattgacct tctgaaaaat cagaagaagc tagctcttcg
    22681 gtgatttcgt catagtaagc caatattgac tcaatggatt cgccgtattt acgtttcaac
    22741 tgtcggatta attctaaacg ttgcgtaact tcttctaaac gactctcatc taactccaat
    22801 aaatcaatct gacgactcat atcgccgatt gcatcttgca gcaaatagta agcactttgg
    22861 atattttcat aaattgcttc atactccaca tctagatggg cgatcccttg cagttctgaa
    22921 acggccaagc caacaccatc tacactgttt tgttcattat tcgtcagtgc tccgtaacct
    22981 gcagctaatc catcagcaat tttttgataa ttgctaagtt tttctctttc ttcttttaat
    23041 ttctcttctt ctccgacttg caaatcagca gcagctattt cttcttgctg gaaatggagc
    23101 atatctattc tctgaacata ggatttttca ttttgctgca cttttcgtac ccttttttcc
    23161 agctcacgat aagagatata ttcttgctga taactctttt tcttctgttg gaaagctaca
    23221 tctccaaaac gatccaataa agcaagatgt gattcaggct ggagcagttc ctgatgttcg
    23281 ttttgtcctt ggatatctac taaataactg ccgattcttc tcagattagc taatgtgata
    23341 atatgaccat tcactcgaca aacatttttt ccagttaagg acatatcccg tcgaacaatc
    23401 agattgtcct catctgtttc gatccctaat tcaaccatca gttcagagaa tccttcttgt
    23461 tttggtagtt caaacaaccc ttcaagcaca catttttctg ctccttgacg tatataatcg
    23521 cttgaacctc ttcctcctgc taacagacct aaagcatcga taataatgga tttacccgca
    23581 ccagtctctc cggtcagcgc ggtcattcct tcgtgaaaag aaagccgcaa ttccggaata
    23641 atcgcaaagt tagtaatact gatttcttgt agcatcacat tcgctcctca acttaaaggt
    23701 aatgcaggaa gtctttcttc aataaaactg cctggtcttc agttcgtgta atcatcaaaa
    23761 cattatcgtc atcattgata atcgaaaata attctttctt gtaacgttta tcaatcaaat
    23821 ttgctgctgc cgaagcattt cccggcaatg ttttcaaaat aacaaatttt tccatttggt
    23881 caactgccac gaaagcgtct tttaatagtt tttccaattt agtatttaca tcttcgtttg
    23941 tttcagctgg tatgctataa cggtagccgc catctgaagc cggtaccttg atcagtttca
    24001 gttcctttat atctctggaa atcgtagctt gagtaacaga tattcctttg cttttcagga
    24061 tttcaacaaa ttcttcctgc tttcggatat cttgctcact caacaatcga gtaatcaaac
    24121 gatgccgatc tttttttctc attacgtctt cgctccttag catattcttc ttaatcaatg
    24181 atagcttatt tccttggtat aataaagaag aatccgcata cttctcactt gattttcaca
    24241 gttacaattt atttatggtc aaactgttcg tgtgcatttg aaaccacttc tgccatatta
    24301 atagctgaat tgattgttcc gctttcgggt actttcctca agtgagccaa gaactcgata
    24361 tttccttctc cccccgtgat cggagaataa cttaattcca gcacatcata tccaccttgt
    24421 gccgcaaatg acaaaatgtc ttctaaaact ttttgatggg tctgagggtc acgaacaatt
    24481 ccgtttttcc ctacagcttc tctgcctgct tcgaattggg gtttgattaa agcaatcact
    24541 tctccctctt cttttaaaat agcatgtaat ggaggcagca tcagttttag tgaaataaaa
    24601 gaaacatcga taacagcaac ttctggaatt cctaaggtaa agtcttcggg ttttgcataa
    24661 cgaaaattca ctctttccat tacgacgact ctaggatctt gtcttatttt ccaagctagc
    24721 tgattgtagc caacgtctaa agcataactc atttttgcgc cattttgcaa agctgcatca
    24781 gtaaatccgc cagtggatgc accaatatct aacaatgttt ttccttcaac agatagatca
    24841 aatgctttga gtgctttttc taattttaat ccccctcgag aaacataagg aagtttttta
    24901 cctttgactc ttaattcact actcactgga attttttctc ccggtttatc aaaccgctcg
    24961 tttttttcgt tgtaaatcaa accagccatc actgaacgtt tggcttgttc tcttgtttca
    25021 aataatcctt gtttgaccgc taatacatct acgcgttctt tttccattct ttccctcatt
    25081 tctccacatc aaattgcttg atcatctggc gcatcagccc gccatcaaaa gaaaaaacgt
    25141 cctcttctaa tttgtctatg atcttgttgg cggataacag ctcaatctcc aacgcctcac
    25201 gtgtcttttc taggcctaat aaacgcgggt aagtattttt ttccatacgt tcatctttac
    25261 cagctgtttt ccctaatgtt ttcgttgtac caatcacatc gagtagatca tctcttatct
    25321 gaaaagctaa acctaaatgt tcggctaatc gttgaagtag caataagatt tcttcaggtt
    25381 gcttcgctaa tatgccacca gccagcaagg cataacgaat caaatgccct gtctttcttt
    25441 catgaataaa tgctaattct tctaaagaaa gctttttcga ttcgccctcg atatctgcag
    25501 cttgcccagc aaccatacct tgtgatcccg cacaaacagc taattgctga agcagcagaa
    25561 gcttaggaga attacctaag tgtgccatac tgattagctg gaatgctcct gtaagcaaac
    25621 catctccggc caatatagct aatgcttcgc cgaatacttt atgattggta ggttttcctc
    25681 tacgaagatc atcattgtcc atcgcaggca aatcatcgtg gatcaaggaa tacgtatgca
    25741 ccatttctaa agcagctgct acttgataga cgggaacatc aataggctca tcaaaggaag
    25801 caacggccgt taaaagtaat aagggacgta ttcttttccc gcctgctttc actgaataac
    25861 tcatcgcatc atagagtcct ctatccgttg tatgctcatc taaaaaagac aagatttctt
    25921 tttctacagc aggtagatga atagctgaaa agtcagttaa tctcatctca gctttcctcg
    25981 tttccgtcaa aatcaacttc ttcatttgat tctgtcatca ttttcgtcaa ggtctcttct
    26041 gctttagcta atgtatcttg gcaatgtttg cttaattcca ttcctcgttt aaacgaatct
    26101 agagctgatt ctaatggaac gtctccttgt tctaactgca tgacaatttt ttctaattct
    26161 tgcagtgact cttcaaaagt tggattttcc atatttttat tctcctttgt ctatctcttg
    26221 tacttgtcct ttaacgattc catcttgata atggatcgtc agcaaatccc cttgatgaat
    26281 ctcggtgaca ctttttacta cttgatcatt taacgtcgta tagctgtagc ctcttcccat
    26341 gattttcaat ggactcaaca aatcaagaga ttgaacagct tgagcaaaac gctgctgctg
    26401 ttgtttcata tattgttgga ttttctcttc taagcgtttt tctagataag cagtttcttg
    26461 gcgagctgct ttaactcgat aaagcggagc cacttgttcc aattggtggc ttaatgcgat
    26521 cgtttgtttt tctttcgcat gataaatttg ctgtgtagct tgatataatc gctgtttcaa
    26581 ctggtctaat ttgatggtct gaccttcata caatctttca ggctgccgaa atacatagga
    26641 acgtcgagca cgctcgaacc gttcttgttt gcgctggatc tggcgtacaa acgcctgctg
    26701 caatctagct tgccgttctt gaatgcgcat caactcttct gacaaaacag gaacagctaa
    26761 ttctgcagct gctgttggag tggcagcccg aacatctgct actaaatcag cgatcgtggt
    26821 atctgtttca tgtcctacag atgaaatcac cggcgtatta gcttgataaa tcgcccgagc
    26881 aacccgctct tcattaaacg gccataaatc ctcaatcgaa ccacccccac gaccgatgat
    26941 cattgtatcg aaattgccca tctcttctac tctctggata ttcctgacaa cattgtccgc
    27001 tgcttggttt ccttgtacaa ctgttgggaa caaaaccaat tggacaattg gatatcttct
    27061 tttcaccgtc gtgatgatat cgcggataac cgctccgctt ggactggtaa ggaccgcgat
    27121 tcgctttggg aaacgtggca gcattttttt aggcccttga aaaaggcctt ccttcatcag
    27181 cttttctttt cgttcttcca attcttgata taaagcacct attccatcag gctccatgtg
    27241 ctctacatag atttgataag aacctcccgc ttcatataat gaaatacgcc caacgacaag
    27301 gactttcatt ccttcttttg gctggaatcg taatttttgg aatgctcctt tgaacataat
    27361 agcagatatt tttgcatggt catcttttaa gctgaaatat tgatgggcat tggcacgtaa
    27421 acggaaattc gatatttcgc ctgttaaata tacgcgttct aaataaggat cggcatcaaa
    27481 ttttcttttc aaatacttcg ttaatgtagt gacagttaaa tattcttgtg ccatctattc
    27541 acctgctcca aaagcttcgg cagattttac ggtttgatac atcagcatcg tgatagtcat
    27601 tggccctaca ccctttggca caggagtgat ccagcctgca ataggctcca cttcatcaaa
    27661 tttcacatca ccgatcagct taccgttagc atcacgattc atccctacat ctatcactac
    27721 agcacctggt tttacgaatt cttttgtgac aaaatgtcca cgtcctattg ctacgactaa
    27781 aatatctgct tcttgtgcca gttcagccag attttcggtt ttagaatgag caacagtaac
    27841 agttgcatct gccatcaaga gtagctgagc catcggttta ccgacgatat tgcttcgtcc
    27901 gataactact gctcgtttac ccgccggatc gatagaataa gcttcaaaca ttttcatgat
    27961 gccgtatgga gtacaaggaa tcttgtccgg tttacccgca aggagtctgc ctaaattcat
    28021 cggatgaaaa ccgtctacgt ctttttcagg gcggatagct aacaggatct tttcttcatc
    28081 tatatgcttt ggcaatggta attggaccaa tataccatga aaactaggat cttgattgta
    28141 tttttcaatt tctgcaagta gttcgtcttc agaaatcgtc tcaggataac gctctaccaa
    28201 tgaatggatt ccgatttctt ttgctacacg ttctttattt cttacataga tttggctggc
    28261 tggattttcc cctaccaata aaacgactaa tccgggatgg ataccattat ctttcaattt
    28321 ttggattttt tcagcaatct ctgcctgcat tttttcagct aattcttttc cgttaatcaa
    28381 ttctgccatc taaccactcc cagatatttt ttgtgttttc attctaccat agaattttcc
    28441 cgatttataa caggtttatg atgattcata gaaaaaagct aggacttacg ccccagcttc
    28501 taattctttc aatacattgg ataaaacacc gttgacgaat ttacgtgaac ggtcatcact
    28561 aaatgttttt gccaactcga ttgcttcatt taaagcaaca gccgctggta catctgtcac
    28621 ataagtcatt tcaaaaatcg cgatacgcaa gatcaccaaa tccatttttg agatccgatt
    28681 gatgtgccaa ttgtttttta aatgtttttc aatcacttta tccaattcat ctttttttgc
    28741 acagacaccg ccaactagcg tatcaagata aacaggaaca aactctgatt catcttcgtt
    28801 gatcatgtcg cggtggtcta attcgattgc gttgaaaatc gcatcttctt ttgttaggtc
    28861 tgcattgaag tctaatggaa ataaagcctg caatgccatt tcccgaatct cgtgtcttga
    28921 taattcttta ttcattctct tcctcttcgt cttcaaaaag ttcttcgatt gttggttgtt
    28981 ctaatttttc aggaacaaca gctactacat ggatattgac ttcagataaa tcgatatccg
    29041 tcatgaaaag gacttgctgg ctgacccgtt cctgcatttc cattgctact ttaggaactg
    29101 aaacaccata ttctagataa cagtaaagat ctactttcaa gccatcttcc tcgtttgcta
    29161 agtaaacacc ttttccgtga gctgcacgtc ctaataattc agtgacattg ctggcaaaag
    29221 ttccgcgcat tccgtatacg ccttctactt tagaagctgc aatcccgata attacttcga
    29281 tgacttcagg agcgatcacg atctctccta aatcttggtt tgcgtttaaa atcaaatttt
    29341 tttcatctgc catttattta ttcctccctt tgcctaagca cgagatacat ccgaattctc
    29401 tatgatgcgg catagtgatc acgtggtaag aaaactgccg ccgatcagct gcaaactgct
    29461 atgaattgga gaaaacaccc aactttatta tctcttacag cttaaaagtt cttctatagt
    29521 gtatcattaa atcgagtgat tttctataac aaagtctgtg aacactgatt tttacgctta
    29581 cttcttctat tgatgaaact ataagatgat caattctttt ggtgaacgag tcaacacacg
    29641 atttccatct tctgtgatca acaagtcatc ctcaatacgt actccaccga ttcctggtaa
    29701 gtagatccct ggttcatcag tgatcacatt tcctggtaca aattgctttt ctgcacgaga
    29761 agaaacgttt ggtccttcgt ggatctctaa accgatccca tgtcctgtag aatgtccaaa
    29821 tgcttccccg taaccatgtt ttgaaatata atctctagca actgcatcta attggacacc
    29881 agtcattcct ggttttgcag catcaataac tgctaattga gcatctaaga ctatttgata
    29941 aatttctttt aatttttccc caggatcacc aatagcgaat gtacgtgtca tatcggaaac
    30001 gtacccttcg taatagcaac caaagtccaa tgtgatcaag tcaccttgtt cgatgatttt
    30061 tttgcttgct acaccatgcg gcatagccga acgcaatccg ctagcaacga tcgtatcaaa
    30121 agaaacacct gaagcgccaa gagaacgcat ataaaagtct agttgatttg cgacttcaat
    30181 ctcagtcatg cccggttgga tcattttcag gatgtgatca tatgccatat ccgcaatatg
    30241 acatgctttt tcgataatag cgatttcttc ttcatctttc acttctctga gttcttcgat
    30301 catctgtgaa acaggtacta attccgcatc gatgatttct tctaaaaccg aatattctaa
    30361 aaatgagact gttgtttcct caaaagctaa tgagtgcagt tcttctttgc gtactaagtt
    30421 tgctacctca tcaaaaatag gtccgacatt tttaataatt tcaaaccctt gagcctgcgc
    30481 tgccgcctgc tcggtataac gaaaatcagt aataaaaaat gctttttcca aagtaataac
    30541 agctaatcct gtcgtacctg taaagttcgt taagtaacga agattgtacg gactggtaac
    30601 caaaaatgaa tccaaatttt cttcttgcat ttttttgcgc aatttttcta cacgtagcat
    30661 cataatgaaa ctccctcttt ctttgcacgc ttttttccat tatagcaaac ttttcttcgt
    30721 acacgacagt taaataaaaa tttccggaaa acttcttttt attttaaata aataaagcca
    30781 aaaaggagtc acagtctcgc tgcaactcct tttagtttac tattttgtaa aaacttatga
    30841 tcgttttcca aagaaaaatg agacaactgc taccaaaatc actgcgccta aaatagatgg
    30901 aataagtgcc atacctgcag cttttggtcc ccattcgccg aaaatcgctt gtccaatact
    30961 tgaaccgact agtccagcaa tgatattcgt tacacagccc atcgaatttc ctttactggt
    31021 gatcgcacct gcaattgctc cgatcacggc tccaacaatc aatgtccata accaactcat
    31081 aatataacct ccttctttgc taatcaattt tagcatatgg gatttaaagg ggacaaataa
    31141 ttggttttgc tatttcatat tttcttctgc aaagtaaaaa aataaagaaa acctcctctt
    31201 agaagttttc tttatttttt acttgcgttt aaacatacta cgaataccac gtttcactac
    31261 atcaaagaaa ctcaatgatt gtaccattct atcttcatct acatattctc tgatcgcacg
    31321 aagcactttt ttaggttctt tggaagaaaa tgtgtatgta ccattttttt tcgtttgaat
    31381 ggcatatcga ggaatccatt ttccctttag catgactgat gctagtacat aatccacttc
    31441 ttcccatgga atttggatat atttgcgtct atctcgcgca tggtaaaact caaaaccatt
    31501 atcacctatc atgatttgcc cgtaatctgt cagacccgta aacgaagtag catcaattac
    31561 caagtctact tttgtattga ttgattgaac cattttctcc ctccagatat ttgtttatga
    31621 acagtatact atataaaaaa agctcgggac aacgcccgag ctatattttt atggattata
    31681 aaagtccgat gacgtgtcca acaacaccga ctacgaataa accaaggatg ataacgattg
    31741 gagaaacctt tttcttaagc aaccacatac ataacaatgt taatcctaat gcagctaaac
    31801 caggaatcaa ttggtctaag ttgtcttgta atgttgttac cttttcagga gaaagggcta
    31861 agccattatt aacttgttca aatgcactct tgattccttc accacctgaa ggcaatttat
    31921 cccattcgat gtaagcacct ttatctaatt ttacagttga tacaactggt aagaatttga
    31981 ttgataccca tctttgtaca agggcagcta atacgaacat ccctaaaatt gaagcacctt
    32041 ttgttacgtc ttgtaacaat ccgccagaaa ggtcatcagt aattttagaa ccagctttgt
    32101 agccaaattc ttgtgtatac cacatgaatg cccaacgaat aatgttccaa cctaagaaga
    32161 agatgattgg tccaaggata tttcctccca tagctaatga agcacctagt gcacctagca
    32221 ttggacgtac tgtaaaccag aacactgggt caccaacacc agctaaagga cccatcatac
    32281 ctactttaac cccttgaatc gctacatcat caacaggcgc gccattcgca cgttcttctt
    32341 ccagagccaa tgtaacccct aaaattggag aagcaatgta tgggtgtgta ttaaagaatt
    32401 ccaagtgacg ttttaatgct gcagaacgtt cttcttttgt tttgtataat tttttgattg
    32461 ctggaatcat agaaaatgcc cagccaccgt tttgcatacg ttcgtagttc catgaacctt
    32521 gaatgaaggt agagcgccac gcaacagcta aacgatcttt ttttgataat ttgatttttt
    32581 cttctgccat tttattttcc tccttcaaga ttaatagtca ttcaaaatgt cgcctaaagg
    32641 gtcaccagag ttgcttccgc ctccattatt tgatgaacca cccattttag aaaggttcaa
    32701 gtaaataaga gctaatgcaa cacctaaagc acctagagca atcaatgtta attgagaaat
    32761 tgcagctaca acgaaaccaa taacgaagaa tggccatact tcttttgtag ccatcatgtt
    32821 gattaccaaa gcgtaaccta cagctacgac cattccccca ccgatagcca taccatctgt
    32881 taaccatgct ggcattgatt caaggaatga ttgcacagtt tgtgctggga taaatagcaa
    32941 cgctgctgct gggaccgcga tacgaacccc ttgtaaacat acagcaatga tgtgccacat
    33001 ttcaacctta cggatgtttc cttcttcagc agccgcatcc atcaaatgaa cgattggaac
    33061 agcgattgta cgtacgatca ttgttaagaa caaccctgca acagccaaag gaacggcaat
    33121 cgcgatcgca gaaggaacac ctttaacacc ttgtccacct aaaactaaaa taattgctga
    33181 tgctactgat gctaatgcag catctggtgc tacagcggca ccgatatttg cccaacctaa
    33241 agcgatcatc tgaagcgtac cgccaagtac gataccagct tcgaggttac cggtaactaa
    33301 accgatcaat gtacatgcca ctagtggttg atgaaattga aattcatcaa gaataccttc
    33361 cataccagct agaaaggcaa ctaatacgac taaaattatt gaaataatag acatgataag
    33421 cctcctataa attttaatat tatttattac gcttttgcta attcagcttt tgcttttttc
    33481 aaaatatcat ccatgttatc gcgtgaatca ttaggtactt tacgtacatc gaatttcaca
    33541 cctttttgtt caagcttctc aaacgcttca acgtcttctt gtcccataga caatacttta
    33601 cttacaacga ctttaccgac cgaatgcgcc attgacccaa catttaattc tttgatgtct
    33661 acgccacctt caacagctgt caatacgtct tcaggatttt caaataaaag caatgctttg
    33721 gtatttccaa aacgagggtc ttttgctact tcgatcattt tgctaatagg aataacattt
    33781 gcttttacac ctggaggagc agcttgttcg attagtctct tacgcagatc atcctttgat
    33841 actgcatctg aaacaacgat aatacggttt ggttgcactg cttttgtcca agctgtcgca
    33901 acttgtccat gtaacagacg tgagtcaaca cgtgctaata catatttgat ttttccgtct
    33961 ccgaccacag ttccttctgg aagtgctcct ttaggttgtg cgtcttcaac tgctgctttc
    34021 ggtgcttctg ccggttctaa ttcttccggt tttactttta caccgtcttt tgctgtttca
    34081 atgatgtgtg ctgcaatttc atgagcagat tccattgaaa agcgtgatgc gtaggcttca
    34141 atcaacattg gtaaattcaa accgctgacg attgcccatt tatctttgtg ctcttcgaat
    34201 aatgtgtttg attgattgaa cggtgttccg ccccataaat caactaagaa taacacttcg
    34261 tcttgattat caaaagaagc aaccgcttct tccaatttgg ctctcaagtc atctggacct
    34321 tcacttggtt tcaaaatcac ggctttgacg ttttcttgtt cgccaaagat cattgaaccg
    34381 gattgtaaga ttccttcagc aaattgtcca tgacttgcta ggataatccc taccatattt
    34441 atacctccta tttacttttg ttgtaacaaa ttcaaaaata ttaataattg ctaaccatta
    34501 caacagaatt atttgacctt ttccaacaac cattcttgct acaccttttt gattttcgct
    34561 agtagttcca tcaaaaggat tttcctgtca gcaggtactt ttcgaatctc taactccact
    34621 tcttgactgt tcaagtaacg aaaggaatca atatcttgtt gatttactga aacaaaattg
    34681 gtaatcattg atttgccttc agtaaaactc ataccgccga tattcaacga cttcaactgt
    34741 acaccgcagc ggacaaccgc ttcaacatct tggggattgg tgaataaaag catcactttt
    34801 gctcgtgata ataatccact atgatagatt tgaatcattt tctgcacggt tattacattt
    34861 gctgtgatcc ccggcggtgc agcttctttt agtagaaact ttctcaattt atcttgagcg
    34921 actacatcgc tgatcacaat gatacgttca acacctgttt gcttcgacca aactgttgcg
    34981 acttgcccgt ggattaaacg atcatctata cgtgccaatc gaatatccat ctgcaaaact
    35041 ccttcttcct cactacttca cagaaacccc tatcatttct gtgtgaacaa ccacctccta
    35101 gcacacacta ctataagcaa aaagcgtgcc aactttttta cgatacacta attgaattta
    35161 aaggattctt aaccaataca caaaaacgat acactataaa agtgtatcgt ttttgtgtat
    35221 tcatggcaca gtgtattctt ttttatctaa ctgattatgg actagacgta gcagataata
    35281 catttcttct atcggcactt ttacttttat ctgatcttcc aacagaacaa ctgaggctgc
    35341 caattggcta taaagaggat cattcgttgt atttgtcaat tcgttttctt ccacagtcaa
    35401 tggttcattc cgaataatac gttcgatcat tcctgctgtg tgcatcacta ggttgatttt
    35461 gaatccatat tcttcatcgc cgattcccgt cgtctgagag atttgtgtag aaaattgcca
    35521 cattggatcg attaacttac taccattaat aaagatgaag ttatcagaga taaactcaac
    35581 acacgtattt ttagctgact cttcatctag ggagatgaat tcagtttcct ccaattctga
    35641 ttccatcagc aactgatcca aaatgacttc gatattttga tcaataaaac gttctaaagg
    35701 aataaatggg gcttggatat tcaaatcagt aatcccagta gtagctatga tctgatattc
    35761 tttttggata actggcagtt cttctttcac ttccacaatc gacaaagcta gaacttctaa
    35821 ttctgtttct tgacgcttgt ttaccgctcg ttcgatcaat tctttgattc tttgtgctgt
    35881 accttcgcca gatgcgcaga tagccaaaac agcttttttc ttatgaatag gttttgcttc
    35941 ctgaacagat tctacagaag atatttctgc gtaacctcga aaatttttca atgattcatg
    36001 caacatatct agatctgtcc ctaatactga gacctttcgt acagtttcca aaactagaga
    36061 agttgttacc atatcaacag tacgtactgg aattccagtg tctctttgaa tttgcgaatt
    36121 aaacgacgca agggacccca tatctactaa gagtatgacc ccgcttcctt catctacttc
    36181 ttgtacattg cgttcaattc ggtccaatgc tgttttcgga tccatatcca aaggcatgtc
    36241 tactgctcgt acgttctctg catctaacaa ctgttggaca acttggacca tactgcttgc
    36301 tgtgctgttt ccgtgagctg caacgactac accgattctg ccgcttgctt gatttgcccg
    36361 taacgagacg agtagaacgg tcaagtaata gtcttcactt tctggaatct ccacttgaaa
    36421 atacgtttcg atataactac gcacttcttt cgcaacagca tattcatctg gaaaatcaaa
    36481 agccatctca cggatgttat cattcgtatg ccgttcttct cctaagttga tttttttcaa
    36541 aaaagaactg atatgcaagc tcatagcata aataaagttt tgctgaaact catatggtaa
    36601 agatcttttg accattacat aaatctgatt ggtcacttct atgattttcg gatctacaaa
    36661 ttctgctaat ttgctgtctg cattaaaaga aaaaccatgg tctttataga aagacttcag
    36721 atggatgttg atatctgtag aaatgaaatg attgattgct tcttgatcca aaccatccga
    36781 cttcagtaaa gctgctttat ctccgatgat atcatataga ttataaggta actcatagga
    36841 gtccgattgg attttcatga tcgtatcatt tggtgctaca gttatctttg gctccaatat
    36901 tttagaaagc tctgccattg cactgcggtt gcttgctaat tgaatcaatc cgctacgaat
    36961 cccttctgtc aaatcatcga ttgtgatcga gatttcgggt gattgcatat gatttagaaa
    37021 tcctcgtgca catactaact ggatatttga cttcaattgt cctatatttc cataggtcac
    37081 actgccgatc aatgcttttg ctacatcttc tgtcaaagaa atctttcgct gcgtgcggtt
    37141 tgcttcgtgg gcaatcatga tttttactaa atcgactttc tcatttgcag gacgtttttc
    37201 aaaagccgga agtttgatat tgattgggat acgccgaaca aaagtttcta atagagaaga
    37261 acctgggtct tctgtggtcg cacctacgat ccgtacattt gcttcatggg atttcgttgt
    37321 ttccccaaga cgactgtaag ttccatgatc cataaagtaa aagatcattt cttgaccttc
    37381 aggcggcaac cggtgaatct catccaaaaa cagcataccg ccatccgctt gatcgataat
    37441 ccccattttt tcttcatctg cgcctgtaaa agcacctttt gcgtacccaa ataaatgact
    37501 catcaaaagc tcagggttat tggcataatc agcacaattg aaaacaatca gttccgtttc
    37561 ctcatctatt acatgatgcg cttttgcaaa atgatacatc gcgtgtgcaa aataagtctt
    37621 tcctgatccg gtaggtcctg taatcaaaca atttaatcct cttggcggat acaaaatcgc
    37681 agcttttgcc tgttccactg aatttttcat gctgccgtta gctccaatga tttttgcaaa
    37741 gatatcctta gtatctattt ggagtgtatt tttttcttca gagaccggcg tttctttgta
    37801 actgactaca tgttttgtaa ctggctttac gtgcgtaacg agtgtttgat atatgtaacg
    37861 tactggtcgt ccgtctgtct ttatcagttt tccttcgcgc actagctgat tcagatcttt
    37921 actgctattt gttcgctgaa tccctaacac ttcagcaatt tcctgtgttg ttactccttg
    37981 atcatattct gcctttgtta aatgagcggt tttttctttg acatacatat aaattcggtc
    38041 aatgcgtttt gccaatggta tcgctccttt tctatagact attgtactta ggcttcaaaa
    38101 cataatacta tgtatttatt tctgtttcgt caactattta cctgattttg ttttgttgat
    38161 aacacgcgga tagatagaaa tgttcaattt aaaagaacga aaaatcccga tacgattttt
    38221 ataatggaaa caagaaaaag aacactgttt accatataat tatttcactg atagcaaaca
    38281 atatagcttc catcaattga aaacgatagt tatcaaaaca tcctgattcc aaattggcaa
    38341 aaaaatatta agtgtaaaaa gacaaagtaa acttcgtagc ctccactaca acttttattt
    38401 ttataaacaa aaaaggctgt gacaaaagtc ttgtcacaac ctctttaatc ataagattca
    38461 attaagcttc ttgagctact ggataaacag acacttgttt tttgttacgg cctttacgtt
    38521 cgaaacgtac tacgccatca acttttgcaa ataaagtgtc gtctccaccg atacccacgt
    38581 ttacacctgg atagattttt gtaccgcgtt gacggtaaag aattgatcca cctgtaactg
    38641 tttgtccatc agcacgttta gcacctagac gttttgattc tgagtcacgg ccgttagatg
    38701 tagaaccgcc accttttttg tgggcaaata attgtagatt catagataac aacatggtct
    38761 gcacctccta tttatcatta attgttttaa tttggatata ttctgagttt tcagcttcta
    38821 tcgattgtaa tcctaataaa agattttcta atagaatctc agcaatattg ttttgttctt
    38881 gtgtcattcc tgaagtgact tcggtataaa gataaccgcc ttcttcttca ttcatttcaa
    38941 caatcggctc aaaaccggct aatgaggcaa tcccgttgac agtgctgatc gctaacgcag
    39001 aaacacccgc acaaacgata tcacttccat aaggaccagc atcagcatgc ccggtcaaag
    39061 tgaatgaaac gatctgacct gcgtcatttc gcttaaatgt tcctttgatc atatataggc
    39121 cttctttctc aaacgctatt aagcgttgat cgcgtcgata accacttttg tatatggttg
    39181 acgatgacct tgtttacggt gtgaatgttt tttaggtttg tatttgaaag taacgacttt
    39241 cttttgtttg ccgtgttttt caacagtacc ttcaacagtt gcacctgcaa cagttggagc
    39301 gcctactttt gtagattctc cgcctactaa gataacttcg tcaaaaacaa ctttttctcc
    39361 tgcttcaacg tcaagttttt caacatagat tgcttgtccc acttcaactt ttacttgttt
    39421 accaccagtt ttaataattg cgtacatgct ataaagcacc tccttctgat atacttagac
    39481 tcgccatccg aagtgacagc atatgctgtg cttattactt gttctgagcg gttgtagttg
    39541 tggagtccac aattacaaca gtactagctt accagaacag caaccgttag tcaactaaat
    39601 ttaaaatcaa aaagtaaaac aaaaattcaa gcaatacttc tgcttttcta tcctcacagt
    39661 tctaccaaat aaaatagtcg cattgtgttt cttttattct tataataaaa gaaaaaggag
    39721 gagcataaat ggaataccca aaagaactga tacaagaagc ttctttaaga atgaacggcc
    39781 gtcctgtaga aggatttatc gctgggcaag gacctcttca tcctaaactg atgctcgtgg
    39841 gagaagcccc agggaaaaca gagattgata cgcatgttcc ttttagcggt caagctggaa
    39901 aagaattgat gcaggcactt tcttcaactg gcttaacacg agaagaggtg tatatcacca
    39961 gtgctgtaag gagccgcccg tatcgcgtga ctcatcggat caataaacgg acacaacagt
    40021 cagaaactgt ttatccaaat cggacaccta ctcgttccga agtatttgcg catgcaccaa
    40081 tattggatta cgaactgatg aaggtccagc caaaattaat tgcgacactt ggaaacattg
    40141 gtcttcaacg tttacttggc aaagaatata cggttaccaa aaatcatggt caactctata
    40201 cagggcctgt cttacaatta acagaacaaa aggatgccta cgaatggtca gaaaaaaact
    40261 atcgaattct tccacttttt catccagcag ctattttcta caatcgctcc cttgtacctg
    40321 ttatccatca agactggcaa gtactagctt cacttttaca gcaatattaa aaagaacaga
    40381 caagaccata aaatcttcag tttacagtct tgtctgttct ttttcattgt tttaatggct
    40441 aattgcagta tatttcgcat gtaaagctgt caacgcatga gcaactgttt gatgttgatg
    40501 cgtgaatgta tgttcccaca agtgagaaag ttgtttttct acttctggct gcaaggaagt
    40561 atagataatc gtttgtcctc tttgctctgc tttttcttga aattcatcaa aaactgcacg
    40621 tgccgtcaaa tcaatacttg gtacgcctcg catggagaaa atgacaccat cgtgatgatt
    40681 tagttcttcc aatttatttt tgagtttttc tgaagacata aaaaacagtg gaccactaat
    40741 atacactact gtccagttat tcagcttttc cgtttctggc agccccattc tttgccaatc
    40801 gatcgtttct aatgaaatcg tgatagcagc gcttttgaca acaaaaaata cacagccgcc
    40861 gatcaccccg atcacgatag caatacttaa atcaaagata actgtacaaa tcatcgtaag
    40921 gataaacaat agaactgctg accaatattt attagtaaat agctccttga tcgtttccca
    40981 ttcattcata cgccaagccg tcacaatcaa aacacctgca agtgctggca ttggaatctg
    41041 tgacatcact ggcgcaaaaa caagcatgga gagcagtaag acaactgcat gtatcatacc
    41101 agctaatcta gtttgtgcac cagatttgat ggccacgctt gtacgggcga tggccgcagt
    41161 agcaggaatc ccgccaaaga aaggaagcaa cagatttcca attccttgcg ccattaattc
    41221 ttgattgcta tctaagggct tatttgccat tcggccagcc gaagcaccac atagcaagct
    41281 ttcaatcatt cctaataaag caatgctaat agctggaact gccacttgtt gcaaggcaga
    41341 aaagctaaaa tctcccatat tcaaacgatt actactgatc aatgtttgtg gaatcttacc
    41401 cacagttgct actggtaaat ggaacaatag cataactgct gttgccaaaa tgatagccag
    41461 cagcgaacta ggcattcgtt tcgcccattt ttttggatag ataaacatcc ctaaaatgac
    41521 tagccctccc atgatcaaag aggctgtgtt tatcggaaat cctatcgttt gataactact
    41581 tagtttttca ataacgtttg taccttccga gtgaacacca aataaattat cgatctgacc
    41641 taatgcaata atgatagcaa tacctgaagt aaaccccgta attacgggtg ctgggataaa
    41701 agaagtcagt gttcctaatc ggaaaagtcc tgctgcaagt aaaaatagac ccgctaaaaa
    41761 agtagcaagc aatacacctt gcattccctt tgttgccgca atagacatca agatagctgc
    41821 catcgcacct gtcggacctg atatttgata aaaaccgcct gataaaccgc caataacgat
    41881 accggcaatg atagccgtaa tcaaacctgc ggccgcatca gctccgcttg ataccccaaa
    41941 agccaaggct aaaggcaatg ctacagcagc tactgtgatc ccagccaaaa gatctttctg
    42001 aaatcttgaa agatcataac ctgaaaattc tttttttagt gaggaaacat aatttttgaa
    42061 cacctatcta actccttttt catgaaattt tctaaaatac cattcattag aatatcatat
    42121 ttcatgagat aaagtaatat ttttcacaaa gtttcctgtt agataaattc ttattttttc
    42181 tatatttata agaaaataga aagcaatgcc aacagagcta gcccaccttg tttgagtaaa
    42241 atccaacgat cgctagtcaa actgccatat aatgcaacaa aaaaaataga tattaatagc
    42301 atacttgtga tttctttaga tgcggaagaa agataagctc cataaagtag tcccactcct
    42361 aatagtccat tgtacactcc ttggttctta aacaatgttt gaacagattt tctgcttaac
    42421 tcttctttac tcattttaaa aaccctactt gtcgtgtcag aatcagttct caccgtttcg
    42481 atatacatga tataaaaaaa ttcaattgca actagagtca ctaaaattct agatagtatc
    42541 gtcatatctt cttcctcttt tctgatgagt tttatttttt tgctccttat aattgtactc
    42601 gataaggatg tttttttatt ttatttgcct ttgaaaacaa acaatattgg cacaaaaaac
    42661 aaagaaaaac cgttagaaac ctcagtttct aacggttttt tagcgtcaca ggagggattc
    42721 gaacccccga ccgtccgctt agaaggcgga tgctctatcc agctgagcta ctatgacaag
    42781 aatcagcgga acatcaataa ttatagataa ttaaattgct tctgtcaata cgaaatagtt
    42841 atttaattag caaataatga attattttgg cccaactact aaatcaatct tttaaaacga
    42901 gacattctcc ttcagctgtt ctaaggttat caattgaaaa caggagctac attgacttca
    42961 tgctactcct gtttatttag atttcctgta tcctttaatt gacttacaaa tatagaaact
    43021 tttacatcaa gttcgaattg agccctcgat tcaatcgatt atctacaggc tgccataatc
    43081 atgtatcaca actcgattgt ttctgattcc tttgtcatta agatcacacc atcacctaat
    43141 ggcaagattg tcgatgtcaa atcgggatga tccatcacaa cacgaagaaa ttcatttaat
    43201 ttacgatgga tagctcgttt gccacgggga atctcttcat ctgggagcaa gatcgttcct
    43261 ccttggaaga tgtcatctac catcaaaacg ccaccttttc ttaacaaacg taagcattca
    43321 ggtaaaaatt caatatattt tgattttgca ctatccataa aaataaagtc atacggacca
    43381 gacaactccg gcaagatatc tgctgcttga ccttcaagca aagtcacttt atctgtcagt
    43441 cctagacgtt catagttttt tttagctttc tcgatcatga catcgaaacg atcaattgtc
    43501 gtaacatgag cgttttctcc gattgtcgtg gccatgacac ttgatgaaaa accgatagct
    43561 gctccgatct ccaagattcg ttcaggctga agctgtccaa gtaaaaattt caaaaataca
    43621 actgtttcat ggggaataac tggtacttct gctacatgag cttcttcttc cagtttgcct
    43681 agctcacccg gtaatttttt ttgctgggta cgcataaaat ccaatagttc ttctttgact
    43741 acaggacgat gcatcatttc attgcgcata ctactcctcc tcttcacaca ttatacgaac
    43801 aactgactaa atctacaagc tgcatgagtg ttttctcgtg tttcatgttc agaagagtcg
    43861 ttggatctct ggaatacatt tatgttttta acattcttac aaaaatttct gtgttcgtct
    43921 ccgcttcttt ttcaactcta caaaggtatt ccatgctgtg attcctgtta ggcaaaaagc
    43981 aatcgtaaag aataacccct gaatccatgc aaatgccgtt tttgaaagga ctagtccaat
    44041 atatggaatc tgaaagatgg gactaccgat aatatttctc ttttttacta ctccaggctc
    44101 aatataagtc tttgtatcag cttttgtata aaaatcttcg tggttgtttt caacggctat
    44161 tacgcgacgc gtcgagattt tttttcctga attttcataa tatgtaataa tatctccgac
    44221 taaaatctca tttgtatttt tcggtcgtac atatatcaga tttccttgtg cataatatgg
    44281 actcatttct ttgtcctcaa taatataagg agtcaaacca aaaattcggg gaaccaagac
    44341 gacgagaaga ataagaatta aaaatataat caacgttata gaacttataa aagttatt
//
LOCUS       DACCXO010000012        44092 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.012, whole genome shotgun sequence.
ACCESSION   DACCXO010000012 DACCXO010000000
VERSION     DACCXO010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.012"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            42..2639
                     /gene="adhE"
                     /locus_tag="IVY70_000603"
                     /gene_synonym="adhC"
     CDS             42..2639
                     /gene="adhE"
                     /locus_tag="IVY70_000603"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP7957762.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2868..3974
                     /locus_tag="IVY70_000604"
     CDS             2868..3974
                     /locus_tag="IVY70_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP7957763.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            3991..5019
                     /locus_tag="IVY70_000605"
     CDS             3991..5019
                     /locus_tag="IVY70_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP7957764.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5025..5504
                     /locus_tag="IVY70_000606"
     CDS             5025..5504
                     /locus_tag="IVY70_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP7957765.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5520..6815
                     /locus_tag="IVY70_000607"
     CDS             5520..6815
                     /locus_tag="IVY70_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP7957766.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6826..7650
                     /locus_tag="IVY70_000608"
     CDS             6826..7650
                     /locus_tag="IVY70_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP7957767.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7815..8714
                     /locus_tag="IVY70_000609"
     CDS             7815..8714
                     /locus_tag="IVY70_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7957768.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(8840..9709)
                     /locus_tag="IVY70_000610"
     CDS             complement(8840..9709)
                     /locus_tag="IVY70_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7957769.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            9862..11322
                     /gene="rhaB"
                     /locus_tag="IVY70_000611"
     CDS             9862..11322
                     /gene="rhaB"
                     /locus_tag="IVY70_000611"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP7957770.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            11319..12575
                     /gene="rhaA"
                     /locus_tag="IVY70_000612"
     CDS             11319..12575
                     /gene="rhaA"
                     /locus_tag="IVY70_000612"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP7957771.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            12589..13434
                     /gene="rhaD"
                     /locus_tag="IVY70_000613"
     CDS             12589..13434
                     /gene="rhaD"
                     /locus_tag="IVY70_000613"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP7957772.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            13436..13747
                     /gene="rhaM"
                     /locus_tag="IVY70_000614"
     CDS             13436..13747
                     /gene="rhaM"
                     /locus_tag="IVY70_000614"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP7957773.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            13829..14980
                     /gene="fucO"
                     /locus_tag="IVY70_000615"
     CDS             13829..14980
                     /gene="fucO"
                     /locus_tag="IVY70_000615"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP7957774.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            15107..15865
                     /locus_tag="IVY70_000616"
     CDS             15107..15865
                     /locus_tag="IVY70_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP7957775.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            15965..16609
                     /locus_tag="IVY70_000617"
     CDS             15965..16609
                     /locus_tag="IVY70_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP7957776.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            16616..17623
                     /locus_tag="IVY70_000618"
     CDS             16616..17623
                     /locus_tag="IVY70_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP7957777.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            17639..18481
                     /gene="kduI"
                     /locus_tag="IVY70_000619"
     CDS             17639..18481
                     /gene="kduI"
                     /locus_tag="IVY70_000619"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP7957778.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            18501..19304
                     /locus_tag="IVY70_000620"
     CDS             18501..19304
                     /locus_tag="IVY70_000620"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP7957779.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(19553..20617)
                     /locus_tag="IVY70_000621"
     CDS             complement(19553..20617)
                     /locus_tag="IVY70_000621"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP7957780.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(20614..21699)
                     /locus_tag="IVY70_000622"
     CDS             complement(20614..21699)
                     /locus_tag="IVY70_000622"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP7957781.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(21712..22689)
                     /gene="mvaD"
                     /locus_tag="IVY70_000623"
     CDS             complement(21712..22689)
                     /gene="mvaD"
                     /locus_tag="IVY70_000623"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP7957782.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(22682..23626)
                     /gene="mvk"
                     /locus_tag="IVY70_000624"
     CDS             complement(22682..23626)
                     /gene="mvk"
                     /locus_tag="IVY70_000624"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP7957783.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESKIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            23948..24601
                     /locus_tag="IVY70_000625"
     CDS             23948..24601
                     /locus_tag="IVY70_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP7957784.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            24686..25591
                     /locus_tag="IVY70_000626"
     CDS             24686..25591
                     /locus_tag="IVY70_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7957785.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(25593..26225)
                     /locus_tag="IVY70_000627"
     CDS             complement(25593..26225)
                     /locus_tag="IVY70_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP7957786.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            26545..27069
                     /gene="infC"
                     /locus_tag="IVY70_000628"
     CDS             26545..27069
                     /gene="infC"
                     /locus_tag="IVY70_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP7957787.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            27141..27341
                     /gene="rpmI"
                     /locus_tag="IVY70_000629"
     CDS             27141..27341
                     /gene="rpmI"
                     /locus_tag="IVY70_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP7957788.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            27394..27753
                     /gene="rplT"
                     /locus_tag="IVY70_000630"
     CDS             27394..27753
                     /gene="rplT"
                     /locus_tag="IVY70_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP7957789.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            28005..29342
                     /locus_tag="IVY70_000631"
     CDS             28005..29342
                     /locus_tag="IVY70_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP7957790.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            29390..31519
                     /locus_tag="IVY70_000632"
     CDS             29390..31519
                     /locus_tag="IVY70_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP7957791.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            31494..32183
                     /locus_tag="IVY70_000633"
     CDS             31494..32183
                     /locus_tag="IVY70_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957792.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(32591..33277)
                     /locus_tag="IVY70_000634"
     CDS             complement(32591..33277)
                     /locus_tag="IVY70_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7957793.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(33657..34097)
                     /locus_tag="IVY70_000635"
     CDS             complement(33657..34097)
                     /locus_tag="IVY70_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP7957794.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(34101..34946)
                     /locus_tag="IVY70_000636"
     CDS             complement(34101..34946)
                     /locus_tag="IVY70_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP7957795.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            35095..36513
                     /gene="pepV"
                     /locus_tag="IVY70_000637"
     CDS             35095..36513
                     /gene="pepV"
                     /locus_tag="IVY70_000637"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP7957796.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(36570..37517)
                     /locus_tag="IVY70_000638"
     CDS             complement(36570..37517)
                     /locus_tag="IVY70_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7957797.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(37740..38090)
                     /locus_tag="IVY70_000639"
     CDS             complement(37740..38090)
                     /locus_tag="IVY70_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7957798.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            38290..39369
                     /gene="pepA"
                     /locus_tag="IVY70_000640"
     CDS             38290..39369
                     /gene="pepA"
                     /locus_tag="IVY70_000640"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP7957799.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            39513..39833
                     /locus_tag="IVY70_000641"
     CDS             39513..39833
                     /locus_tag="IVY70_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP7957800.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            39855..40319
                     /locus_tag="IVY70_000642"
     CDS             39855..40319
                     /locus_tag="IVY70_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7957801.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            40524..41129
                     /locus_tag="IVY70_000643"
     CDS             40524..41129
                     /locus_tag="IVY70_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP7957802.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(41168..42457)
                     /locus_tag="IVY70_000644"
     CDS             complement(41168..42457)
                     /locus_tag="IVY70_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP7957803.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            42885..43364
                     /gene="rlmH"
                     /locus_tag="IVY70_000645"
     CDS             42885..43364
                     /gene="rlmH"
                     /locus_tag="IVY70_000645"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP7957804.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            43567..44092
                     /locus_tag="IVY70_000646"
                     /pseudo
     CDS             43567..44092
                     /locus_tag="IVY70_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177294.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
BASE COUNT        13989 a         8005 c         9417 g        12681 t
ORIGIN      
        1 aattatttaa caaataacca tgacccatta ggaggataat gatggctaaa gtaatagaaa
       61 aagaaaaaac gaagacaatt gacgtacaag caatgattga tgatttggca gaaaaagcaa
      121 atgcagcatt gaaagaaatg gaaagctttg atcaagaaaa agtagaccat atcgtgcatg
      181 aaatggcaat ggcagctttg gatcaacata tgccattagc gaagatggct gtagaggaaa
      241 caggccgcgg tgtttacgaa gataaagcaa tcaaaaatat gtatgcgtcc gaatatattt
      301 ggcataatat caaacatgat aaaacagttg gtgtgatcaa tgaagatgtc caaaaagggt
      361 tgattgaaat tgctgaacca gtcggtgtgg tttgcggagt aactccaaca acgaatccga
      421 cttccacaac gattttcaaa tcaatgatcg cgttgaaaac aagaaatcca atcgtttttg
      481 ctttccatcc aagtgcccaa aaatcttctg cagaagcagc acgagtggta cgtgatgctg
      541 ctatcgctgc cggagctcct gaaaattgta tccaatggat cgaacatcct tcgatcgaag
      601 cgacttctat gctaatgaac catccaggta tcgcgatcgt cttagcgaca ggtggagctg
      661 gaatggtcaa gtctgcttac tctacaggaa aaccagcttt aggtgtaggt ccaggtaacg
      721 taccagctta tattgaaaaa acagcaaaaa ttaaacgtgc agtgaacgat ttgatcgttt
      781 cgaaaacatt tgataatgga atgatttgtg cttctgaaca agctgtgatc gtggataaag
      841 aaatctatgc agcagtaaaa gcagaattcc aagcacacca agtttatatc gtaaaaccag
      901 atgaattgca aaaattagaa gatgcagtga tgaacgaagg aaaatatgcg gtcaatccat
      961 caatcgttgg acattctgcg atggaaatcg caaaattggc aggaatcagt gtaccaaaag
     1021 gaacgaaaat gctgattgct gaattggaag gcgtaggccc agattatccg ctatcaagag
     1081 aaaaactttc tccagttctt gccatgatca aagctaacaa cacggaccat gcgtttgatt
     1141 tgtgtgaagg tatgttgaat cttggaggat taggacatac agcagttatc cattcagaaa
     1201 acgaagagtt gcatgtcaaa ttcggtcttc gaatgaaagc ttgtcgtatt ttagtgaata
     1261 caccatctgc agaaggcggc atcggtgata tctataacga aatgattccg tcattgactt
     1321 tgggatgcgg ttcttatggt aagaactctg tttctcgtaa cgtctctgct gtaaacttaa
     1381 tcaatgtcaa aactgtagcg aaacggagaa ataacatgca atggttcaaa ttaccgccaa
     1441 aaatcttctt tgaaaagaac tctctattgt acttagagaa aatggaaaac gttgaacgtg
     1501 tcatgatcgt ttgtgaccct ggtatggtcc aattcggtta ctgcgataca gttcgtgaag
     1561 tattggcacg acgtaaaaat gatgtgaaga tcgaagtctt ctcagatgtt gaaccgaacc
     1621 catcgacaaa taccgtatac gcaggaacga aactgatggc agacttcaag ccagatacag
     1681 taatcgctct tggaggcggt tctgcaatgg acgcagctaa gggaatgtgg atgttctatg
     1741 aacacccaga cacatctttc tttggtgcaa aacaaaaatt cttggatatc cgtaaacgga
     1801 catataagat cgacaaacca gaaaaaacac aattcgtatg tatcccgaca acatcaggga
     1861 ctggttctga agtaacgcca tttgcggtca tcactgacag tgaaacacat gtgaaatatc
     1921 cgttagcaga ttatgcattg acgccagatg tcgcaatcgt cgatcctcaa ttcgtcatgt
     1981 cagttccagc atctgtcaca gcagataccg ggatggatgt gttaacacat gcaatcgaat
     2041 cctatgtttc agtcatggct tcagattata cacgtggatt gagcctacaa gcaatcaagt
     2101 tagtctttga ctatttagaa aaatcagtaa aaacacctga tatggaatca cgtgaaaaaa
     2161 tgcacaacgc ttctacaatg gctggtatgg cgtttgctaa cgcattccta ggtatttgtc
     2221 actcagtcgc acataaaatc ggcggggaat acgggatccc tcatggacgg acaaatgcga
     2281 ttcttttacc gcatattatc cgctataatg caaaagatcc acaaaaacat gcgatgttcc
     2341 ctaaatatga ctatttccgt gcagatacag attacgcaga tatcgcaaaa ttcttaggtc
     2401 tgaaaggcga aacaacagag gaattagtcg aagctttagc aacggctgtt tatgaattag
     2461 gaaaatctgt cggcatcaac atgagtctaa aagctcaagg agtcacacaa gaaacactag
     2521 atacaaccgt agaccgtatg gctgaattag cttacgaaga ccaatgtacg acagctaatc
     2581 cgaaagaacc attgatctct gaattgaaac agatcatcat tgatgcttac aacggataag
     2641 caaaagaaag cgtagaaaaa agataagaaa atagatgaaa aagaacttgg aaaacgttgt
     2701 ttctccaagt tcttttttta atttccaagt aactgaaagt tacttttgtt atttaacatt
     2761 taggtcaatt ttagacagtt tttgttcaaa aagcgatatt tatagtggac ggtattactg
     2821 ctatattaag gatataaaat gaaagcgttt aaaaaggtgg cgtaaagatg attattaaag
     2881 aagagatttt tgtacctaaa gaattagtga aagaaaaaat tgacctgctc gttgaaaacc
     2941 tgacaacgat caaagacgat aatggtgaat ttttgttgga ctttgatgga ctgaaagtag
     3001 atgacaaaag ctggacagtg tggaattggc cacaaggtgt cggtctatat ggtatttata
     3061 aaaattacca gatgacaaaa aatcctcgcg cttatcaagt ggtcaatgaa tggtttgagg
     3121 atcgtatgga agaaggcgct ccgcccaaaa acgtcaatac aatggcaccg ctattgacta
     3181 tggcttactt gtatgaagac acgaaagatt cacgctattt gccttattta gaacaatggg
     3241 cagaatgggt catgaatgac atgcctcgga caaatgaaga cgggttacag catgccacat
     3301 atggaccgga aaataagaat cagttgtggg acgatacatt gatgatgaca gtgcttcctt
     3361 tggcaaaaat cgggaaactg ttgaatcgcc cagaatattt agaagaagct cgttaccagt
     3421 ttatgatcca tatcaaatac ttgatggata aacgaacagg attgtggtat cacggctgga
     3481 cgtttgaagg aaatcataac tatgccgaag cattttgggc tcgtggaaac tgctggttga
     3541 cgattgctat tccagagatc attgaaatct tagaattatc gaaagaagat gcattgcgca
     3601 aattattgat cgaaacactt gaagcacaag tgcgtgcatt aaaaacttat cagtctgaaa
     3661 gcggtttatg gcatacgttg ttggatgatc cttcttctta cgtagaatct tctgcaactg
     3721 caggatttgc ttacggtatt ttgaaagcgg tccataaacg ttatttacca caagaatata
     3781 aagaagtcgc ttatcgtgct atccaaggat tgcttgaaca aatcgatgaa aaaggagaag
     3841 ttcaaaacgt ttcaatcggg acaggaatgg gggacagttt agatttttat cggaatatcg
     3901 gaatcacagc aatgccttat ggacagtcgt taacggtgct ttgtttgaca gaattgctcg
     3961 tctcttattg ttaaaagaaa ggaaacccat atgaaaaaaa catcactgct gtttgcagca
     4021 agttcgctta gcctaggtct gctaggtcta agcggctgcg gaaataattc tagtgtggca
     4081 actgtcgcag atgcagatgt aaatgagata gttaccttgc gctttgctta tgcaagcaac
     4141 agccagccag taattgatgc aatgaatgaa tttgggcgtt tagtaaaaga aaaaacagat
     4201 ggttccgtac agatccagta tttcccagat ggacaactgg gtggagaacg tgaactgatc
     4261 gaattgacac agtcaggagc agtagatttc acaaaagtaa gtgcctcagc gttggaaagt
     4321 ttctctaaag attattcgat tttttcagtt ccttatctat tcaatgatga agaacatttc
     4381 tttaaggtga tggataatga agaaataatg gagccaatct accaatcgac caaaaattta
     4441 ggatttaccg gattgaccta ttatgattct ggtcagcgca gcttttatat ggtagacggt
     4501 ccgatccata cgccggaaga tttgaaggga aagaagatcc gtgtgatgca aagcgaaacc
     4561 gctatcaaaa tggtagagct tttaggcggt tcgcccgtac ctatgggaag tgatgaagtg
     4621 tacacgtctc ttcaatccaa tttgatcaac ggatcagaaa acaatgaatt tgtcttgtat
     4681 acagctggac atggtggtgt ggctaaatac tattcctatg atgagcatac acgtgtgcca
     4741 gatatcatca tcatgaatga tgccatcaaa gaacagctaa ctgatgagca ggaaaaagcc
     4801 attgaagaag cagcgaaaga atcaacggtt tttgaaattg aagcttttgc aaaagcaatt
     4861 gaagaagaaa aagctttagc acaagaagaa catggagtcc aattcaatga tgtagataat
     4921 acaccgtttc gagaggcagt tgccccactt catgatatat tcaaaaacga tcctacctac
     4981 aaagaccttt atcaaaagat ccaagagcaa ggagcgtagc tgtgatgaaa atgattttaa
     5041 acaaggtatt agagatcctg ggagctgttt tgcttgttgg gatggtcgct gttgttttat
     5101 ggcaagtatt ttccagaacg gttttaggaa atccaaatac ggtcacggaa gagcttgttc
     5161 gattcggtct tgtctggttt gccatgttgt cttccgcgta tgttgtggga caaaaagcac
     5221 atttagccgt aacggtttta agtgaaaaat taactggcaa aaaaagtaaa attttagaag
     5281 ttgttgttca aagtttattt ctggtatttg ctgcgatgat catggtgtat ggcggatgga
     5341 atgctgtcac attgacaatg gggcagatct cgccttcttt aagtattcct atgggatatg
     5401 tttatctgtc agtacctgta tcgggggtac tcatcattat ctatagcctg atcaatctaa
     5461 tcgatacatt aagcaacaag ccagcaattc aagaaaaaat ataaaaatgg aggaataaaa
     5521 tggcaataga agccggatta gtgttatttt ttgtatttgt tgtgttgctt gtattaggga
     5581 tgccgattgc tataagtatt gcattctcat cgatgatgac tttattgtta gttataccgt
     5641 ttgatgtttc tgcttttagt tcagctcaaa aaatggtggg aagtatcaat agtttttcgc
     5701 tgatcgctat tccgtttttc gttctctcgg gcattatcat gaataatgga gggatagcta
     5761 agaaactagt ggattttgcg atgttgttcg tcgggcgcgt gccgggggct ttggctcata
     5821 cgaatgtgtt agggaatgct ttatttggtt ccatctctag ctcggccatt gccgcttcta
     5881 ccgcgattgg cggagtgttg atccctcagc aagtggaaga aggatatgac cggaagtttg
     5941 cgacagcggt caatatcgcg tcagctccga caggtatggt cattcctcca agtactgctt
     6001 ttatcatgta ttcacttgtt gctggtggtg cttcgatctc tgctttgttt atgggaggtt
     6061 atctcgtagg cgctttgtgg gcgctaggaa tcatgctagt agcattcatc gttgctaaaa
     6121 agaataaata ccctgttgtc gaaaaaggac agatggctca tgctggaaaa ataataaaag
     6181 aggcgattcc tagccttcta ttgattgtca tcattatcgg tggtatcttg acggggatat
     6241 ttacagcagt agaagcttca gcgatagctg tcatttactc cctgttgatt gctatgtttt
     6301 actataaaac ggtaaaacta aaagatatac ctaatatgtt gtttcaatca gtagcaatgt
     6361 cagggacgat catgttctta ttggctactt cttccatgat gtctttcgcc atggcgttta
     6421 ccggtattcc tcaggcaatt agttcgttga ttatgggcgt aacggataat aaatttatta
     6481 ttctgttatt agtcaatctt gtgttattac tggtgggtat gtttatggat gtggcgcctg
     6541 ctatcttgat ttttacacca atatttttac caatcgtcac aagtgtaggg gttgatcctg
     6601 tacattacgg gttgttttca atcatgaatc tgtgtgtagg ctccatcacg ccgccagtag
     6661 gtacaggact atatgtcggt gcaagtgtag gaggcgtaaa agcagaggca atgctgaaac
     6721 cgcttcttcc tttttatggg gttatcttag tcgtcttgtt tttgattact tacttcccgc
     6781 aaatcgtcat gtggctaccc aatatgctga gttaggagaa tagttatgtt gaacattaca
     6841 attagaggac atgatctttc cgaagtccaa acgatagaag atcttgcaga aaaaacaaaa
     6901 gaacaaggga ttcatacact gcagctcgcc ttaggtcttt cgtttcctga aataccttct
     6961 ggcgcaaatg agatcaattc tgggatggga aattatgtca aacgagtttt agaaaagcaa
     7021 gaagtgaccg taggaatttt aagttgctat atcaatatga ttcatccaga tttaacgata
     7081 cgacaagagt tgctgacaaa gttcgagcag tatcttcgct atgcttcttc ctttggtgcg
     7141 tcgatggttg cttcggaaac tggatgtgtt ttgccggaaa tccaatatac ggaagaaaat
     7201 ttcacagatg aagcatttgc tgaagctgtg tccgttatcc gtcgcttggt aaaagctgga
     7261 gaaaaatacc aaatgatggt ggggatagaa ccaggtttga atcatccggt gtattcttta
     7321 gcaagggtag agcagctgat ccaagcagta gattcggact atctaggaat cattttagat
     7381 ccgaccaatt tgatcacaag tagtgattat caagaacaag tccaattggt agaagaagct
     7441 tttgaacgtt ttggtgaaaa gatatgtgcg gttcatttga aagattttcg tgtcgaacag
     7501 gaaaaaatcg tgccggtaaa tttgggagac ggtgtgatcg agtacacaaa aatcaaagaa
     7561 atcatcatga agaatcgtcc gtacctgtat gtggttcttg aagaaacaaa agatgatggg
     7621 attcgttatg gaagaagtct tcttgaataa tgtaaagaag acgttggtta actaatattt
     7681 agaatggagg gggcggggct agaagatgcc acgtctcttc ttttttataa gagtatataa
     7741 aagcagctct ttttgattac aaaaaacgtg gtataataaa attgtgcata agtgaacaaa
     7801 aggaggtgcg gcacatgctc caaaagaaaa tcagcggtga acaagtcaat acgatggttt
     7861 ctgatcagta tgaaatctat catgtcaaag atgtcgtcag ccaaaataaa acgatctatc
     7921 atcatcatga tttctatgag atccatgcta cattaaaggg agaagcgact ttttatttga
     7981 acggacgaca atttacgatc aaagcaggaa gtgtgctgct catccattcc aatgatttgc
     8041 atcgtatcgt ccgccaatct tcagatgtat tcgagcgtgt ctatatcttt gtcacccctt
     8101 cttttctaga aagtcgttcc acgccatggt ctaatctatc ggcttgtttt caaccaatgg
     8161 gagagaagag gagtcgtgta ttgcaaatag aacaggaagt attgaagcaa aaattatcat
     8221 ttatcgacca tcctcctgat aaaaaaattt acggtgaaga cataaaatat gaacaagctc
     8281 tggtagaata cttgattttt ctcaatcaag ttgtacaaaa agaagagagt gcgcaagaac
     8341 agaatgctgt agttcctaat gagcgtgtgg aacaaatgtt acaatatatc tcaaaaaatt
     8401 tatctcatcc gctttcttta gaggaaatgg aaaagcagtt ttttgtcagc aaataccata
     8461 tcacacgaga atttaaaaag tacactggat ttacgtttca tcagtatgta atgaagaaaa
     8521 agctgatcta tgccaagcac ttgttgcgag aataccgcag ctctagtgct gtatatagta
     8581 aaagtggttt ttcttcttat ccgcattttc tgaaagcatt caagagagag ttcggcataa
     8641 cgcctaaaga atttttgaaa cgtgatacac agaaacagtt ggttcattat gaacattttg
     8701 aagatgaaca atgagaatga tcactaaaaa ataaccgcag tagctgctgt tgtttacata
     8761 gaagcagaaa ctgcggttat tttattttta tctatatatt tcaaaatatt ttgaaaaaat
     8821 gacgtacata ttatatgaat cagctgaatt tacgaaagtc tctcggcgat agtcctgttt
     8881 ttgctttgaa caggcggcta aagtaagcaa aattctcaat ccctatttct tcggaaatca
     8941 ttcgaaccgt aaattctgta taccgaagaa ggtctttagc gtaagaaagt tttttgctga
     9001 tcaaataatc gattggtgga gaaccgataa atttggaaaa atctttgctc agctgatatt
     9061 tattcaataa gaattgttgt tctaattgat caagtgtaat cgtttctttg aaatgttcgt
     9121 ctaagtattg cttcagtttg taaacgtagg cgggaatatc ctcttcttcg aaatctagct
     9181 gatacttttg caaaatcagt tcattcagca actcgtggat agctacagag gactggaaat
     9241 cggtacgggc attcgtgcct tgttgcatgt ggatcaattc tgtgatcaaa tggtggatct
     9301 tgttttctga tggatctcct gttgaatgga aggtgttatg gccgtctttt gtgaactcct
     9361 gataaaatga ttggacgttc tgaccattca aatggatcca atccatttcc catggttctt
     9421 cgctgatcgt ttgataacgc tgatattctt ggcagtcaat aaaaaataag tctccagctt
     9481 ttagctgttg ctgattatca ttatatgtaa gcgaaccttt gccagacaaa gtaaacttta
     9541 tcaagtaaga aggtaaatgt gctcgttcag tatagtaagg gattggtgct ttgaagtaac
     9601 cgatttcttg gatataaaaa aataaattcc tgcttctttg tgtaggcgtc aagatcactc
     9661 tttttgagtc ttccgaccat gagtggttgg ctttttccca gtaatccatg taaacactcc
     9721 caacttgtca agataatgca attatatacc aaaatagtgt gaagcaaaat ataagattct
     9781 gtattatgat agcattgtca ataaatgata acgctttatt aataaagtgc taatcaacaa
     9841 taaagtaaaa gaggtggaga aatgaatcca acatttgttg ccatcgatat cggtgcatca
     9901 agcggacgac taatgaaaag tacgagggat gcaaaaggtt ctttgatctt ggaagaagta
     9961 caccggttca aaaacggctt tcggaacaaa gacggtcacg atcgctggaa gatcgattat
    10021 cttatcaaag agattttgat tggatttgaa aaattaaagc agacagggat tgatcgcctt
    10081 tttgcgggga tcgatacatg ggcagtcgat tactgtctga ttgatgaaca aggggatcgg
    10141 ataaatgatc cgattgctta tcgggacaag cgtaccaatg gagcagtaga acagttttct
    10201 gaaatctatt ctctagataa gttgtatgag cagacaggga ttcagatcca gcctttcaat
    10261 accttgttcc aattgtttgc tgaagaaaaa gaaaatttgg caaaagcaca taaattgtta
    10321 ctgatacctg attatttagg gtatgtcttt actggtaaca tggtgacgga gaaaactaat
    10381 gcatctacta tgcagctgct gaatgcaggg acaaaagaat gggaaataga acttcttgaa
    10441 aaaatcggtg tctcgaagga attatttcca ccattgacag agccgggaac gatactagga
    10501 gatctgcaaa aagaacaatt ttcaaaatat gatttgccag atgtgacctt tatcacagta
    10561 gcaagtcatg atacggcgtc agctgtatta ggaacgccag gtcaaggaga acgttggggt
    10621 tatatcagca gtgggacatg gtcgctttta gggattgaaa caaagatcgc caatgtttct
    10681 cgcaatgctt tttctgaaaa ttatacgaat gaatggggtg cccataacac ggtgcgtttt
    10741 ttgaaaaaca tcatggggat gtggttgatc caggaagttg cccgaatgca agagtacgag
    10801 tacagttatg cggaattggc agagcttgct gcaaaagaaa aaccgctgca gcaagtgatg
    10861 gaaatcaatg accctcgatt tttgaatccc ccaaatatga tagaagagat tcagcaatac
    10921 tgttttgaaa cagagcagcc agtccctgtc tcatcgggag ctatcgctcg ttgtgtgtat
    10981 gataatcttg ctctttgtta cgcacaagag ctgatcaaag tagaacactt aactggaacg
    11041 aaattggaaa agctgcacat cgtagggggc ggatcgaata atcgtctact gaatcaattg
    11101 acagcagatt atgccggaat cattgtagaa gcaggaccta gtgaagccac agcaatcggg
    11161 aatgttgcca tgcaaatgct cagtacaggt cagttttcga atatagaaca agcaagaaat
    11221 gtaatcaaac agtcgtttcc atgtgaaact ttttacccag atgaaaataa gcaaaacacg
    11281 atggaatggt acgtgaataa aatcaaagga gtaatcgtat gaacacgatt gaaagaaatt
    11341 acgaacaagc aaaagaaaaa tatgcaacta tcggtgtaga tacagatgct gtactagaaa
    11401 agatacagga catcaaaatt tctatgcatt gctggcaagg agatgatgtc aaaggtttct
    11461 tgacaccgga tggcgaattg accggtggaa tcatggcgac agggaatttt ccaggagccg
    11521 cacgaacacc tgaagagctt agacaagatc tggagaaggc ttattcgttg ataccgggca
    11581 aacacaaatt gaaccttcat gctatctatt tggatacaga agaatctgtc gatctaaatg
    11641 aaattgagcc gaaacatttt gaaaaatggg tagagtgggc aaaaaaagaa gaaatcggtc
    11701 ttgacttcaa tccgactttc ttttcgcatc caatgatgaa agacggcttt acattggctc
    11761 atccagataa agaagttcgt gaatactgga tcgaacacgg gaaaagaagc cgcaaaatcg
    11821 cggagtactt tgggaaagaa actgggcaaa caagtatcaa taacttttgg gtacctgatg
    11881 ggatgaaaga taatccgatt gatcgtttta ccccacggga acgtttaatg gaatcactcg
    11941 atgaaatttt tacagaagag ttagatgaac ggtatgtaca agatgcagta gaaagcaaac
    12001 tatttggttt aggcgctgaa gcttatacag tcggttccca tgaattttat atgggttacg
    12061 ggttgactcg tgacaaactt gtttgtctag atgctggaca ttttcatcca acagaagtga
    12121 tttccaataa attatcttca ttagctttat tcagcaaagg aatcatgttg catgtttccc
    12181 gtccagtgcg ttgggattca gaccatgtgg ttttgatgga tgatgaattg caggaaatcg
    12241 ctaaagaatt agtccgtaac gaattgttgg aaaaaacaaa tatcggtctg gatttctttg
    12301 atgcaacgat caaccgtatc gctgcttggg tcatcggtac gagaaataca caaaaagcac
    12361 tgctgaaagc tatgctggaa ccagttgagc gattgaaaga aatggagctg gcttttgact
    12421 ttacttcaag aatggcttat acggaagaat tgaaagattt cccgtatgcc gatgtatgga
    12481 attatttctg ttacaaaaat caagttccag ttggtttgga ttggctggaa gaagtccagg
    12541 aatatcaaaa agacgtgttg gaattaagaa aataggagtg tggctatatt gaaaaaaata
    12601 gatattttac aagcgcctta tgtgaaagaa atgatcgaaa cgacgtcgaa tctttatcgc
    12661 ttgggatggg atgaaagaaa tggcgggaat atttcttatt tactaaaaga agaagagatc
    12721 acaccgttct tggatcctga aaaagtgatc cgccgcatct cgatgatttt tgatgccagc
    12781 cgtttagcag gatgctattt tattgtaact ggttcaggaa gatattttaa aaatgtagcg
    12841 agcgaacctg ccgagaatct tggaatcgtt cgagttgctg aagatggcaa aacactggaa
    12901 cttttgtggg ggctggaaaa cgaatgcctt cctacaagcg agttgccaag tcatttcatg
    12961 agtcatatcg cccgtttaga agtagaccca gataatcgga tcgttatgca ttgccatgct
    13021 agtcatctat tagcgatgag ttttacgcat gaattagacg aacgttcctt tagtcgtacg
    13081 ctatggcaaa tgtgtacgga atgcttagtt gtttttccag aaggaatttc catcattccg
    13141 tggatggttc cgggaacaaa tgagattggg gaagcaacag ctgaaaaaat gaaagaaact
    13201 cgattggttt tatggccaca acatggaatc tacgggtcag gaaaagacat ggatgaagtc
    13261 tttggtttga tcgagacagc agaaaaggca gcagaagtct atacgtacgt caaagcgcaa
    13321 ggaccaatct tgcaaacaat cacggacgaa aacctgtggc atttagcaga tgcgtttggt
    13381 gtcactccaa aagcaggtta tttggaggaa gttcatacga aagcaggtgt ctgacatggt
    13441 gaaaaaagct ttttgcatga aagtttaccc agaaaaacaa gaagaataca aaaaaagaca
    13501 tgatgaactt tggccagaaa tgcgtacagc acttcgtgaa cacggggtca tcgactattc
    13561 gatctatctt gatccgagta caagtttgtt atttggttac ttagtgatcg aagatgaaaa
    13621 gttatgggaa aaaatggcaa atacatctat caatcaaaaa tggtggaagt acatggaaga
    13681 tatcatggag acaaatccag atgcctctcc agtcagttgg gaattgatgc cagtctttga
    13741 actgtagcaa caagcaactg ggcaaccggt tgcttggggt tctttcgttt ttattgtgaa
    13801 aaataataca aattaggagg aatagaaaat gagtaatcga atgattttaa atgaaacatc
    13861 gtattttgga aaaggagcga ttgagtcgat cgtccctgaa tttcagcagc gaggattcgg
    13921 caaagcagct gtcattactg acaaaggatt gatcgagcac ggtatagcaa caaaagtaac
    13981 agaacttcta gataaagcag caatcgctta tgctttatac gacgggatcg tacctaatcc
    14041 aacgatccaa aatgtaaaag atggagtggc ttttgttaaa gaggcagagg cagactgttt
    14101 gatcgcaatc ggtggaggtt cgccaatcga tacggcaaaa gccattggga ttattttgac
    14161 gaatccagag ttttcagacg tcattagttt agaaggtgtt gctgatacga aaaatccatg
    14221 tttacctata ttagctatac caacgacttc tggaacagca gcagaagtaa cgatcaacta
    14281 cgtcatcaca gatgaagtaa atcaccgaaa atttgtttgt gttgatcctc atgatatccc
    14341 aatcgtcgcg ttcatcgaca gcgatatgat gatgggaatg ccgaaaaaat tggctgcatc
    14401 aacaggtatg gatgctatga cgcatgctat tgaagggttt atcacaaaag gcgcttggga
    14461 aatgactgat atgctgcatt taaaagcaat cgaaatcatc ggccattctt tggaagcttc
    14521 tgtagatggc gatcaaaatg ggcgagaaaa aatggcatta ggacagtata ttgcgggaat
    14581 gggcttctca aatgtcggtt taggactggt acacgggatg gctcacccgt taagtgcatg
    14641 gtacaacatt cctcatggcg tagcgtgtgc agcacttttg ccaactgtca tgaaatataa
    14701 caaagaatat actggagaaa agtaccgtga gatcgctctt gttctaggga ttaaaggagc
    14761 agctgaaatg tctttagaag atgtaagaga agcggcttgt ggtgagatcg atcggttgag
    14821 caaagcggtt ggaatcccag aaaccatttc tgaattaggt gtaaaagaag cagatattcc
    14881 agcaattgca gaagatgctt tgcgagatgt atgcacacca ggaaatccaa gagaaacgac
    14941 agtagaagaa atcattgctt tatatcaaag tttgatgtaa aaacgaagcg gatcttcaac
    15001 attaaattgc ctgtaaagag aaatggtagt ttatatctgg atatctcttt taaattgatg
    15061 gtatttccgc tataattttt cttataaaga aataggagaa tattatatgg aaaataaaaa
    15121 gccttatgga actgtattga tcaaagcctc taaaatacta gactatttag cggaaaacca
    15181 agatgtctct ttgcagacga tcgcaaaggg tgtgggaatg acctcttcta cagtcttgaa
    15241 aatactagat actttgttga tgatcggcta cgtaaataaa aattcagaga aaaactatcg
    15301 tttaggtgca aagcttattc gctacgcgaa taaaaacatt gaacaaatcg atttggtaga
    15361 gttgacttta ccgtttttag aacagctgca gcaaaagatc gatgagacta tccacttagg
    15421 ggtattggac aacaatgaga tcttgtacgt gaacaaacta gatccaaaac atcaaacgat
    15481 ccgcatgtct tcaaaaatcg gaatcacccg accattatac aattctgcga tgggcaaagc
    15541 cgtattagca gaattcagtg aagagcagta cactgattat cttgaaaagc atccgttgat
    15601 cccttacaca gaatatacga tcaccaatgc attacggttg aaaaaggaaa tcaaaacggt
    15661 aaaagaaacc cacatcgcat ttgacgatga agaaatcgaa cgtgatattt tttgtatagg
    15721 agcctcaatc gtgaaaaacg aacaaatcat tggtgcattt agtgtaagta tgccgaaata
    15781 tcgtttaaca gatgagacaa aagaacaaat aatcagtgcc ctcaagcaaa caaaaagcga
    15841 aatcgagcaa agaattcaaa aataatgaaa aaagcgtttg caatttttct tttctgccgt
    15901 ataatatggg tatagatttc caagttataa aataaaattt ctaaatagga aataggtgga
    15961 gaagatgaaa aaaattgata ttttatctcg attgaaaaac gccggagtga ttgcggtggt
    16021 acgcggaaaa agcaaagaag aagctttgaa tgcttgccac gcaatcatca aaggtggtct
    16081 aactggtatt gagttgactt ttacagtccc tcaagcagat caagtaatca aagaactttt
    16141 gtcattctac aaagaccaac cagaaatagt gattggcgca ggcacagtat tagacgctgt
    16201 cacagctcgc ttggctatct tagctggcgc agagtatatt gttagccctt cctttgatca
    16261 agaaacggca gagatgtgca atctgtatca aattccttac ttaccaggat gtatgactat
    16321 taccgagatc aaaacagcgc tgaaaagcgg ggtagatatc gtcaaactat ttcctggaag
    16381 cgcttatgga ccaagtatca tttctgcctt caaggcgccg atgcctcaag tcaatatcat
    16441 gccaactggc ggtgtgagtc tggacaatat gaaagagtgg ttcgatgctg gtgtcgtaac
    16501 agtcggagta ggtgggaacc ttttagcacc cgcagctaca ggagattttg acaaagtaac
    16561 ggaagtcgcg caacagtatg cagcaaaaat gaaagaaatc aagaggtagc tgcttatggg
    16621 aaaagtagtg acactcggag aaatcatgtt gcgattgtcc acagagcagg gaacccgtat
    16681 cgattcaagt gaacgattcg ctgcccatta tggcggaggg gaagcaaacg tagcaatttc
    16741 tttagctaat tacggacatg aagcgatttt tgctacaaaa gtacctgata acgcattggg
    16801 aaaagcagtc aaaaagcact tgcaatgtta tggagtagat acgaaacagg tattatctgg
    16861 tggatcaaga ctaggtactt attatatgga aactggtagt ggcgaacgcg ccgcctcagt
    16921 gatctatgat cgtgccggat caagttttgc tgaaatggat catttggaat ggtcgctaga
    16981 ggagttattc caaggaatag atatatttca tgtttcagga atcaccccag ctttgtctaa
    17041 aaaatggcaa aaaatgacga aacaggtgat agaagctgca aaaaaagctg gctgcctgat
    17101 cagcttcgat atcaactatc gtggaaaatt atggacacag caagaagcca gcgaagtaat
    17161 ccatcagctt ttacctttag tagatatctg ctcagctgga aaaatggatg ctctttattt
    17221 gttaggtatc gagaaagcgc cagaagcaga agaacaacct ctaatttatt attatcaaaa
    17281 aatgcaggaa aagttcccga atattcaggt gttttattcg accaagcgta ccgttcattc
    17341 agcaagtgaa aatgaattga aggggacatt gtggatgaac catgaatatg tggaatccca
    17401 agtccataga atcaccccaa tcgtggatcg agtaggtggc ggagatgctt ttgcaggtgg
    17461 cgtgctccat gggattttat ccaatatgtc tccgcaagaa atcattgatt ttgctacggc
    17521 agcttctgcc ttgaagcata cgatccatgg ggactgcaac caatttagtc aagaagaagt
    17581 cgcacaattt ttagcatgcg gctctggaaa aatcattcga tagaaggagt agaagaaaat
    17641 gcaagaaatg gaaacacgtt atacacacag tccagaagat atccgtcatt attcaacgga
    17701 acaattgaga aaagaatttt tagtagaaaa ggtatttgaa ccagggaaaa tcagtttgac
    17761 ttatacgcac aacgatcgaa tgatctttgg tggagtgaca cctactgaaa aagaattaga
    17821 aatcacatta gacaaagaac taggtgttga ttacttctta gagcgtcgtg aactaggtgt
    17881 gatcaatatc ggtggaccag gattcatcga gatcgatggg caaaaagaag aaatgaaaaa
    17941 acaagacgga tactatatcg gaaaagaaac acgacatgtt gtattttctt ctcaagatgc
    18001 tgaaaaccca gctaaattct atattagttc tgttccagct catcataaat atccaaatgt
    18061 aaaaatcagt atcgaccaag taaaaccaat ggagactggt gaaggcctga cactgaatga
    18121 acgtaaaatc taccaataca tccatccaaa tgtatgcgaa agctgtcaat tacaaatggg
    18181 ttatacgatt ttagaaccag gaagcgcttg gaatacgatg ccttgtcata cacatgaacg
    18241 tcgtatggaa gcttatgtct actttgatta tgccaatgaa gatacacgtg tgttccacat
    18301 gatgggcaaa ccagatgaaa caaaacattt agttgtagac aatgaacaag ccattatttc
    18361 gccaagctgg tcgatccatt caggtgtagg tacaagcaat tactcattca tctgggcaat
    18421 gtgcggagaa aatatcacct atacagacat ggatatggtg caaatggatc aattaaaata
    18481 agcataaggg agagaaaatg atggaattta gtatggatat gtttcgttta gatggaaaag
    18541 tagcattagt caccggtgct gtctatggta ttggatttga gattgcacgc tctttagcta
    18601 cagcaggagc aaaaatcgta tttaataact tgaatcaaga atcagttgat gaaggtatag
    18661 cgcattataa agaagcaggc atcgatgcaa aaggttatgt ctgcgatgtg accgatgaag
    18721 aagcggttca agcgatggtt caacagatca aagaagatgt aggttcagtt gatattctag
    18781 tcaacaatgc agggatcatc aaacggacac cgatgatcga aatggatgcg gcagacttcc
    18841 gccaagtaat cgacgtggat ttaaatgccc cattcatcgt ttcaaaagca gttattccgg
    18901 atatgatcga aaaaggcggc ggtaaaatca tcaatatctg ctccatgatg agtgaattag
    18961 gacgcgaaac ggtcagtgct tatgctgcgg caaaaggcgg attaaaaatg ctgacgaaaa
    19021 acatcgcttc tgaatacggt caatacaata tccaatgtaa tggaattgga ccaggttata
    19081 ttgctacacc gcaaactgct ccattacgcg aaacacaaga aaacggtgaa cgtcatccat
    19141 tcgatcaatt tatcgttgga cgtacgccag cagcacgctg gggcgagcca atcgatttgg
    19201 ctggaccatc tgtcttcttg gcttctagcg catctgattt cgttaacggc catgtcttgt
    19261 atgtggacgg aggcatactt gcctatatcg gaaaacaacc ttaaaagagc tttagtacac
    19321 tgtttagtgg agaggttgtg aaatcagcgg attgaacgaa taccgaagga cagctgatta
    19381 aacatcattt attcgaaaaa agaggctggg acagaagcat ttaactccaa gaaataagaa
    19441 ggaattcacg aaaattgctt ttcaaatttt tgtgaatttc agcttatttc cgaaggagtt
    19501 gcttttactt ccgccgtttg tacgtgttta gagcgtgaga caaaagtgtt ctttactttt
    19561 gtcccacgct cttttctgtg cgtcttccat acttcactaa gtcgatccct cttgcttcgc
    19621 accaatcctt cactggtccg gaaaagatca gagattgttg gtgtagggcc gcggtattcg
    19681 ttgcgccgac catagtataa agcagacgaa gctcttcttg ccattgcttc atcagtataa
    19741 tagtttcttc tacgccgtga ttcatcagat gcgtcagtac ggttcctgaa gtacccacgg
    19801 catttgcgcc gagacaaagg gctttaaata tgtcataggc attgcggata cctccggagg
    19861 ctaggatttc catagaagtg tctgcttcat ttgcttcaag caatgaagag actgtcgatt
    19921 gcccccagtc agctaaataa ctcagttccc gcttggagcg acgggcgttt tcgatctggg
    19981 tgaagcttgt tccgcttcga ccgctgatat caactgtatg aactcctaaa gcagctagat
    20041 cattgagcgt ttctcttgtc atgccaaaac cgacttcttt tacgatgagg gggacgtcaa
    20101 tggctgtttg agtttcttga ataagtactt tccaattggt aaaatcacgg tctccctcag
    20161 gcataaccaa ctcttgaggt gcattcaaat gaatctgtaa ggcatctgcg tgaaacaatc
    20221 ggatagcttc tttggctctt tcgacagaag tccctgcacc gatgttggca atgattttcc
    20281 cgtgaggata ttcttgtcgc ataatcgtat aagtatcggc taaagaaggg actttcaagg
    20341 cagcgctgac tgaaccagtg gcaatcatca aatctgcttc tcttgcgacg attgcaagat
    20401 cttggttgat tttttttgtt ttttcgcttc cgccagtcat cgcattgata taaaatgggc
    20461 tgcttagtgt aaacccaact gcctgtgtac ttagatcgac atcagaaact gctatttgcg
    20521 gcaaagggtt atggatgatc cgtacgaagt caaattcgtt tttttgttta tcatgaaagg
    20581 ctttagctag tgaaacatgt tcgtcttttc gattcatcgg ttttcctttc tttgatcgct
    20641 atagacatgt aacggcagtg gagtgatttc tgctttttcc catgcactca ttaaaggaag
    20701 aatgccagat ttctggtcaa caattacgat tccgcaatcg cccccacctg caccagaaga
    20761 ttttgcggct ccttcatact gttcagctaa attacacaat ttgttcaaag caggcgtttc
    20821 gatgacgacc ccagtgattg cagataaatc atgcagtaat tgtcggtttt ttcgaatcat
    20881 ctgttggatc aacgttacat tattttcttt aaaccctttg atcatttcgt tgacacattc
    20941 tgtactattt tttaagaaaa gctgataagc caccatttta tcttctctcg aacggtgaac
    21001 ttgatcgacc aaatcagaag tagaggcagg gctacccgtc caaccaatca ataaacgtaa
    21061 atcttcagga gcaatcaatg gctcaatgga tagacctggc caatccaggg caagtaactc
    21121 actgatagaa tgctgagttt cttgttcttg gagccaagga tggtcgaagg ttgagaaagc
    21181 gatccagcca ccataacagc tagcagcgat gtcgccgcag gaaccattat cttgaactgc
    21241 taaattggct agtgctgcaa tcttgaaaat ctccaactga gataaattca acgcgtaaaa
    21301 aacattcaag gctttgaccg ttgcaacggt cacggcaccg ctagaaccca atccatattt
    21361 ccggccattt gaactgtcta actcacttgt tactttcaga tcataaaagg ataaaagaat
    21421 gtttttttct tgtgcatact tttcagtcaa gcgaatagca gcaaggatat aatgaaaagg
    21481 attttcccga atatctaaaa ctaattctcc gttgcgtctt gtccaacgta caggcatccc
    21541 actatattgt gcagattgga tacttccgac ttttcgtgca gattctacag tgactgtcac
    21601 gaattgatcg actgcagcga taactgctgg atggcctgtt tcaacaactg catattctcc
    21661 ggcaatatag agtttgcctg gtgcagatac ttcaatcata aaaatctccc tttattcaat
    21721 aatcgcaatt cctggaccag catgtgcagt aattacttgt tctttgctaa ataatttggt
    21781 aaatgtttct tgtacctcag ataaatggct gttttccact aaaactttga cattagggcc
    21841 agcatccatc gtaaagtagc atggaatgcc ttgctttctc aattgtcgca ccgcatccat
    21901 cgcttttaag ctgtcaggag accagtaagt gaatggaggc tgggcagcta aagtcgttcc
    21961 atgcattttt agtccgtttc tttccatcgt ttctccgaga agttggaaat cttttgtttt
    22021 gattgcttgt ttcaattgat atagatcccc ttcaacggaa tctaaccagc cttgataaaa
    22081 actggatgtt tcgactgtcc gacgcatccc atttctgctg gacacttctt ttttctggtc
    22141 gttgatcaaa acgaaaacca ttgctaaatc gtcttcgaaa gaatcggaag ggactggctt
    22201 agcgtaagaa cttaagtcgt catggccttt ttcccattcg acgaaaccac cgaaaatact
    22261 tcggcaagct gaaccagacc cgcgtcgagc aaatcttgaa agagagagat catctaatcc
    22321 aagcttaaga gcagtattgc aagcgcctgc taaagcagct agcccgctgg cagaggaggc
    22381 taatccagct gcggtaggaa cgaaattctg actgattact gaagctttta gtgagaggcc
    22441 ggcttcttta cgaaaaaggt caagaaactg actgactttt tttgttgctt tttcgtcttg
    22501 caaggtgcca tctagataaa attcatctac catatagcta tcagaaaaga tgacttctgt
    22561 ctctgtataa aatgcatcca atgttaacga aaggctgttg ttcattggaa ggatgagttc
    22621 ttcatttttc ttaccccaat atttgattag agcaatgttg gtatatgcgc gtgctttgcc
    22681 tttaaacata ggtatgtact cctaatcctt gtatccaagt ttcagcagca cccgcatctt
    22741 caagtgcttg actgatttct tgggcttttg tttttgtttg tgctaatgca atcatacagc
    22801 caccgcgtcc accgccagtt aatttggcac ccaatgcccc ggtatctttt gctgtttgga
    22861 tcaatagatt aagaaaatca ttgctgattg tcagcttttc cagaagtgat tgtgattcat
    22921 ccattgtctg tgccaacgtt tctggtgaat tttcaatgat tgcttgtttt gcttgcttcg
    22981 tcaagtatcc taatttttgg atcatttgtc cagtttcatg aggctgggtt tcaaagaggt
    23041 gagcgacatc tttgacggct tctcttgttt gtcctttgat tcctgtatca gcgacaatca
    23101 aaaaagcatc gatgttcaaa gaaaagtagt cgaaaggatg cccttttgta aaataaatcg
    23161 gctgcaagct gctagtagcg gctgcatcga ttccactagg attaccatgg gcgatttttt
    23221 ccgaaagctg gacattttct aatagtattt cacgagacaa aggaaatgct aagtaatcat
    23281 aaaaagcacg agtgactgct gtggcgactg cagcgcttga tcccattcca cgttcagcag
    23341 gaatcttgct ttcaatggtc agttgcaagt ttttttggat gtcatgctgt ttttttaagt
    23401 gaatgaataa ttttttgata ttttttaatg catgaggagc ttcggctaaa tttcctgaat
    23461 agtaggaaga aaccagttga tcgtccattg catccagctc ttcaaggaat gcggtgactt
    23521 ttgttgcata gaaaggaaag gcaatcgctg gttctccata aacaaccgcg tgctcgccca
    23581 tcaatatgat ctttccgctt gattctcctt ggccatagtt tgccataatc taactccttc
    23641 tcacgtaaaa gtgattttta agcaaaaaat aaagcgccca aaacgagttg cgctgcttca
    23701 cgaatttttt tgtccactaa gggtctcaaa aaacattcct cctttatttt actaaaacca
    23761 tctcagaagt aaaactaaga atacgttttc taaagaaaaa acactttttt cttaagaatt
    23821 ttttgacttt ttttcttctt tgactagaaa aaaatacaat tgacactact agatattggg
    23881 ggtatattta aaaaggttgg agttaaacac aatatttagt gatgaagtga gctggaggta
    23941 aatgagcgtg atcgttttag caggtacaat tggtgccgga aaatcaagtt taacagaaat
    24001 gatggctgaa cactttgata gtcaggcatt ttatgaatcg attgatgata atgaagtttt
    24061 gcccttattt tatgcaaacc ctgaacagta tgcttttttg ctgcaaattt attttttgaa
    24121 taaacgtttt gcaagcatca aacaagcaat gaaagatgac aataatgtat tggaccgttc
    24181 gatttatgaa gattcactgc ttttccatct aaatgcagat ctaggaagag caacagaaac
    24241 agaagttcgt gtgtatgatg aactgctgga aaacatgatg gaagaacttc cctatgctgc
    24301 ccataaaaaa catccggatt tattagttca tattcgtgta tcttttgata cgatgttgga
    24361 gcgtatcgaa aaacgtggtc gttcgtatga acaattgtcc tttgatccaa gtctatacga
    24421 ttattacaaa gaattgaaca gacgttacga tcaatggtat gaagaataca aagaaagtcc
    24481 aaaaatccaa atcgatggag accgctataa ttttgtggaa gatcctcaag caaaagaaga
    24541 agtcttgaaa atgatcgaag aaaaattagc agagattcgc caaaccaaag tagccatttg
    24601 atttacagtt gctcaaataa agggtaaacc tttatttgag cgactttttt ctttatactg
    24661 gaaacaaatg tcaggaggat agagtatgcg ggaaaaacac tattttaatc aagaccgtaa
    24721 tttttggatc aatgtacagc aaaacgatgt gctagctgtt aaaaacttaa aagaagaata
    24781 tggcattagt gacgagatgc tgacttattc tctagataaa aatgaacggg cgcgcgtaga
    24841 gtacgattct tttgatgaag cgttactgct tgtatcaaat gttccgcacc agcaaaaagt
    24901 cgataatcat tatgaaacta gtcctatcgc ttttatttta aaagaagacg gtttgtttac
    24961 atttaccacg ccgaataccg agtacgtgat tcgactaatt cgatcgcttt tggaacgaat
    25021 gccagacatg agtgtataca gtttattgtt tcgtacattg tttttgattt ctgattcctt
    25081 ttttccttta atcgaggaag tgaatagtga acgtcagcgg cttaatctga aattaagaga
    25141 aaaaacaacg aacaagaatc tgctgcaact ttcagattta gagatcggtt tggtttactt
    25201 agttacagga acaaaacaaa atgtagtact tttagaacaa atcaaggcat tggctattta
    25261 tcgcaagttg tcagaaaagg aaaaagaaga gttggacgat gcgctgatcg aagcacgaca
    25321 agcagttgaa atgacgaact tagcttcgca gattttagac cagctctctg gcacttataa
    25381 taatttgctg aataacaact tgaatgacac gatgaaattt ttgacggtct ggtcgctgat
    25441 cttgaccgtt ccaactattg tgacaggatt cttcggaatg aatctccagt taccctttac
    25501 ccattccgtt ttcggatggg gaatcgcctt gatcatcagt ttggtcttat ctatttggat
    25561 gcttattgca ctgtggcggc ggattcgttg atctagtctt tgatctgcaa accaagggct
    25621 tgggcgatca caagtgcttg ctcttggttg acgattagtc ccgccaaaga atcttgagat
    25681 aaagcgattg ctgtgaagaa attagtcgta aagtctaatc ccttcagaga agttcgaaac
    25741 cagttcgaac cggtcaactg attgtcagac aaaaaaaggt ttttccaagt gacctcataa
    25801 aattcgctgt cttctaattg acaatgatcg aaggagacag ctttcaagtt tgtactacta
    25861 aaggaagcca tcgaagcaat acaatcagtg aaagtacagt ctcgaagata actttcggca
    25921 aaattcgtac ctgttaattt acattgatga aacacgactt ggtggaaact gccaccaatc
    25981 cactccagat tggaaaaatc acatttttca aaaatgacat tactgcattc aaaatgacgt
    26041 aaaactgttt tttgcatcgt aagtttttgg atatgactct ctcttaatac catatttttg
    26101 gcatcgagat agctttggtc acccccgttg atcagaagac cagagaagat ctcctcatct
    26161 tctaaataaa actctccctt tgtaagaaga ggaagagaag gtggaatagg ttcgattttt
    26221 ttcatgtggg ttcacctcgt ttttctttag tatagcaaag aagcgggaat cactgctaga
    26281 aagtctgcta atctaatcat tttcagaaaa ggaagacaaa ctgtatgaaa aacattgaca
    26341 aaaagattag tttttgctat actatgatgg ttgagaaata aaagcagaag cacccgcttc
    26401 tcgccttagt tgataatgtc actgggctag atagttttca ctggttctat taggtgaagt
    26461 acgcgcgggt tctgtaagag aactcgtttt tttattgccg tttattcggt aagcttttct
    26521 ttctctcata atatttggag gtgaatgacc atagcaaagg atatgatggt taacgacggc
    26581 attcgtgcac gagagttacg attgatcggt tcagatggtg agcaattagg agttaagaca
    26641 aaagcagaag cattgaatat tgcagaacaa gcaaacttgg atcttgtgtt agttgcccct
    26701 ggtgcgaaac caccagttgc gcgaatcatg gactacggta aataccgttt cgaacaacaa
    26761 aagaaagacc gcgaagcgcg taaaaaacaa aaagtgatca atgtaaaaga agttcgctta
    26821 agtcctacga tcgacttgaa tgacttcaat acaaaacttc gtaatgcacg taagttcttg
    26881 gaaaagggcg ataaagtgaa agcttctatc cgtttcaaag gccgtgccat tacccataaa
    26941 gaaattggtc aaaaagtctt gaatcgctta gctgaagaaa ctgcggatat tgcaacagtg
    27001 gaacaaaaag cgaaaatgga cggacgcagc atgttcttga cgctggcacc gaaaaacgac
    27061 agtaagtaat ttgaatgact aactggctgt tggacagccg atagtcggat aacgaaagat
    27121 tttgaggagg aaatatagtc atgccaaaac aaaaaacaca ccgcggatca gcaaaacgtt
    27181 tcaaacgtac tggtaaaggc ggattgaaac gcttccgcgc gtttacaagt caccgtttcc
    27241 acggcaaaac aaaaaaacaa cgccgtcaat tgcgtaaagc aagcatggtt tctagtggcg
    27301 acttcaaacg tattcgccaa caactagcaa aaatgaaata agaagtacaa ataacagaat
    27361 ctgaagattt atttttatta ggaggaatta tacatggcac gtgttaaagg tggaacagta
    27421 actcgtaaac gtcgtaaaag agtgctaaaa ttagcaaaag gttactacgg ttcaaaacac
    27481 actttattta aaacagcaaa agaacaagta atgaattcat acaactacgc atatcgcgat
    27541 cgtcgtcaaa agaaacgcga cttccgtaaa ttgtggattg ctcgtatcaa cgcagcagct
    27601 cgtatgaatg gcttgagcta ctcaaaatta atgcacggtt tgaaagttgc tgaaatcgac
    27661 atcaaccgta aaatgttagc tgacttggct gttaacgatg cagcagcatt tactgcattg
    27721 gctgaccaag caaaagaagc tttggctaaa taagctaaac aattataaag aggaaagaag
    27781 ataacgaaaa gttatgttct ttcctctttt tttatttaca atcctccatc aaatgaaaca
    27841 ggacaataac gtataagtct aaaatagcat aattaaggtt ttttaattga aaactgaaca
    27901 tttatggtca ccctttaatg aaagcgctaa ctacataagt tagaatgagt ttgactgata
    27961 tattagtttg cttatcagaa aaacaaaatg gacggaggaa taacatgagt gatattatta
    28021 ttggtatttt gttagttatc tcattctttt tcatggtctg gtactgtgta aaaggctaca
    28081 acttgatggt cggctttgcc atcatggcta ctgtatggat gggattagcg ctagtcggaa
    28141 acacgttttc accaaacccg gcgatggaag ggcaaggagt aattgatgtg ttgacgcata
    28201 tctatacgac ggggccagct gagtacgcca aatcgatctt ggtaaatgtc ttctttggtg
    28261 cctttttcgg gcgtgttcta gtagatagcg gaatcgcagc taccctgatt cgaaaggtag
    28321 ttgaacttgg aggggacaag ccaagaatca cgatgagctt actatgtatt gtaaccgctg
    28381 ttatattcat gtcgatgaca ggaatcggac cagtgatttc tattgcagtt attgtgttgc
    28441 caatcttgat gtcattaggt atttcagtac cggttgcact gttctcattt atgggctcga
    28501 ttatggctgg aatctttgct aatatcgtca atttcaaaca atatcaaacg atttatgctg
    28561 gtttcaatcc agcggctgaa agctatactt ataatgacta tttccaaatt ggtatgattg
    28621 ggatgattgt aagcttagtt gtggtactaa cggtcgctaa tatatcaatg aacaaaaaga
    28681 aacgctatgc aatggcagca aacgtaccag ctgaaggtgg cgacgcacca atgatctcat
    28741 ggttagccgt attattacca gttttagggg tcgttctttt agatcttcca atcatcttag
    28801 gttttattct agcaggtatc tgggcattgc tgtttactgg aaaattgcgc ggaggttata
    28861 aagaaatctg tcgtcagttt gcgaaattat ttacagatgg cgcagtagat gttgcgccaa
    28921 tggttggttt cttgatgacc ttagcgatgt tcaataattc agctgcgtat gcttctcctt
    28981 atttttcagc tatttttgga gattggatac cgcaatcacc attagtcttg gcaatcgtat
    29041 ttgctatatt gacaccacta ggatttttcc gcggtccaat gaatctagtc ggttcaggct
    29101 cagctatttt agctgtcgta ttagctgtta atccaacgat gtcgccagcc tttttgttcc
    29161 cgttatttgc aatcacaacg attgctcctc agcatttaga tattacacaa tcttgggttg
    29221 cttggggatt aggttataca aaagtaactt ctcgagaata tatgaagaaa tccatcccaa
    29281 ctggttggat catcggagcg atcttatgcc tgatcacgtt cttattgtac gggaacgcct
    29341 agtaaacaaa acgtcgataa aaacgattag atgaatggag gaaaagaaaa tgaaacgttt
    29401 tgtgcgaatg ggaattgatg ttggcggtac acatacgaaa gcagtcgcaa tcgataatgc
    29461 gacgcacgaa atcatcggaa aatcttcagt gaaaacaacc cacgatgatg tccgtggagt
    29521 agccgcaggt gtcgttcagt cttttcaaaa ttgtttgctg gaaaataata tcagtccaga
    29581 agatgttgtg tttgttgcac attctaccac acaagcgact aatgcactga ttgaaggaga
    29641 cgtagcaaaa gtcggcgtaa tcgggatggc aaaaggtgga ttagaaggtt ttttagcaaa
    29701 acgacaaact agattgaacg atatcgattt agggaataag aaaaaaatag agattgtcaa
    29761 tgcattctta cctgtcaaac acttaaatgt agaccgtgtt tcagaaacga tttcttcttt
    29821 agaaagagaa agagcggagg tcttggtttc ttctatggca tttggagtag ataatgggga
    29881 accagaacga gttgtttatg aagcagcatc ggtaaaatcg atccctacaa cgatggcttc
    29941 tgacatcaca aaattatatg gattgacaag aagaactcgg acagcagcaa tcaatgcatc
    30001 tattttacca aaaatgctag atacggcaac ctctacagaa gattccgtac gagaagcagg
    30061 ggtcaatgtt tctttgatga ttatgcgcgg agacggcgga gtaatggaaa tcaatgaaat
    30121 gaaaaaacga cctgttttaa ccatgctttc cggtccagca gcatcagtca tgggctcttt
    30181 gatgtacttg agagcgtcta atggtgttta ttttgaagta ggcggaacaa caaccaatat
    30241 cggggtcatt aaaaatggac gtccagcaat cgattattca attgtaggtg gccacccaac
    30301 atatatctct tcattagatg ttcgggttct aggtgtggca ggcggttcga tggtacgggc
    30361 taatcagtca ggaatcatcg atgtcggtcc acgttctgca catattgccg gtctagatta
    30421 tgcagtattt acggagacag aaaaaatcaa aggtcctaag gttgaatttt tctctcctaa
    30481 agaaggagac cctgctgatt acgtcaaggt agtgatggaa gacggagaag aagtgactat
    30541 tacaaataca tgtgcagcca atgtccttgg attagtgcaa gaagaacatt tttcttatgg
    30601 aaatgtccca tctgcacgaa aagccatcca agcattagct gattattgtc atacgacggt
    30661 tgaagacatc gcagaacaga tcatggaaaa atcctatgct aagattgaac cggtgatttt
    30721 agaattggca gacaagtatc acttggagaa agatcagatt tctcttgtag gtgtaggggg
    30781 cggcgctgcc tcattgatta cttactttag taataaaatg ggtgtgaaat attcgattcc
    30841 tgaaaacgcc gaagtgattt cttctattgg tgtagcactt gcaatggtcc gagatgttgt
    30901 agaacgtatc atcccttcgc caagtaaaga agatatacga tcgttgaaaa atgaagcgat
    30961 gaataaagcg attgaatcag gcgctacacc agaatcgatc gaagtccacg tagaaatcga
    31021 tccacagact tcaaaagtaa cggctatcgc tactggttct acggaagtga aagcaaccga
    31081 tctaaccaag gagatcacaa cagaagaagc actagagctt gctgcagagg atatgcgatt
    31141 aaataaaaat gaagtttgct tattggaaaa tacacctttc ttttatgtat gcggcgaaca
    31201 gaatcgttca aaaaatgctg gaagtcttcg gatcatcgat caaaaaggat ttatcaaggt
    31261 ccaacgtgga catgcttcat gtatgaaaac aactgcagcc aattatatga cagctgtcga
    31321 acaactttgg gaagatatgg cagtttatca aactgaattg attgcccgac cagaatttta
    31381 tctctgctta ggagcaagag tctctgattt tacagcgacg gatctggaac aactacagct
    31441 tttgatggat ctggaagtat ctactatgga accggaggaa gaagtgatcg tcgttgccgg
    31501 aaatatcaaa caaacataag ttagaagagt tactaaacga attacaacag atccccgaag
    31561 aaatttgggg attctaccag tttcggcgag atttattttg gaaaaagatt cctttgtcca
    31621 agcagaaaat tcttatacaa caatcaatcg attgtggtat cgaaacggca tgtagcatca
    31681 aaaaaaagta cccttttgcc gatgtaggag aaatttgtga acaaatggct gttccaatcg
    31741 tttcctgtga atcagagcaa ataaacgaac gaatcacttt tgcaacttat gcagaagatg
    31801 aagggattcg attgatgacg gagccgttag aaaaattaaa atgttcagga ctagcttcga
    31861 tctctaaaga aacagcgcaa gctttgatta ttggacatga actatttcac cacatcgaag
    31921 cttctgttaa gggaatttat acgcaaagcg aaaaaattgt gctgtggcgg ttgccttttt
    31981 atacacatca atcaaacatt cgtgcattga gcgagatagc agcgatgagt ttttcaaaag
    32041 agatgaatca aagcgatttt tcgccttatg tattagaggc agtcttactt tggccatata
    32101 atgaaattcg tagccaaggg atcttagaag aaataaaaaa aatagaaaaa agatgtacgg
    32161 agtatgattt agattacaaa taaagagact gtgacaaaag tcgtgtcaca gtcctttttt
    32221 tccgaataaa cgatgttctc ctgtcaactc ctcggtcaca agtcaaaata ttcaacaaac
    32281 atgagaagct gttttttgaa attgttcctt gtgactcgga gatgaacggc aggctcacga
    32341 cctcttggaa aacggtgttc aaaagcttgc atctgcggta ataagtccag ccaagttaaa
    32401 aacataagaa gctattttta cttggctgtt cttattactt gatgcagggc agctttttca
    32461 caacctctca ataaacggtg tgcaatcagc tgaccctcgg tattagctca aaaaaaacgg
    32521 caaaatatga ggaactattt tgccgtttct ttctctaata ctcaggtcaa aacgctgatt
    32581 ccaaaacctc ttatttgatg ggtctgtcct gaatatcttc aaaataatcg gggtaattat
    32641 gaatcacgta ttcttgctcc tcgataatca tatgtaaatg catcgtgcaa agaaatccga
    32701 cttcatcaaa gttatgatca atcattgatt gaagcattgt ctgatgttca tctaatactc
    32761 gaccccagtc taattctgaa atcgtcaaac gtaaccatct gaaacggttc aagtgcgtac
    32821 tataactttc tagccaatcc caaatttctc gttttccagc aatttcataa caagttcggt
    32881 gaaaatcagt atccaaatca aaaaaagctt ttttattttg agcgattact gcctgatcgg
    32941 tttgttctag tattttttct agtttagctt gatcttgctt ggtcattttc gcactgcatt
    33001 cttgcaggat cggtttttcc aacttttccc gcgtataacg ggctaatgaa gcagatcgga
    33061 tatcgatttt tgaaacataa gtgccggact gtggaatggt ttttacaagc tcttgattgc
    33121 gcagttggat cagtgcttct cgaatcggtg tacgcccgat agctagttgt tcttctaaca
    33181 ctttatcgga gattttactt ccaggtgcta actcggaata aatgatttgt ttacgtatag
    33241 attcataagc ttgctgttgc aaattattga taaccattta accttccttt ctatgcagat
    33301 ataatctatt ataacataaa tcgcttacaa atcgtgtggg cataaaaaca cagtctgcaa
    33361 aaaaagccct gataggcatg cctattagag cctcgaaaag aaaaatattg aatcaatctg
    33421 gtcgtacctc ttggatcggt ttgacacgac cgccgattct tttagcaaaa gcttcggatt
    33481 cttttttgtc tttgaaaatg agcagattgt ctaagttttt ttcccgagtg cgacagccag
    33541 ttttatcgat gtaaccgtgc attactttaa cgacatacat ggggatcacc tgcttttttc
    33601 ttttcattat acgaactact cgacacaata caagaaaaat gcttagacgg tctttattaa
    33661 gaatttacat catccaaaga agcgtacgct tggaataact gatctaactc cggattgact
    33721 ttgctcagct tcactaatcg ttgcgaatca gaaagtaaaa aacagtgttt actactgcca
    33781 gtagttttta attttttgtc ttcagttccc acaatttcgt agctaaaatc caaccgtgtc
    33841 cccgtatact tttggatagt tggatggatg ctcacttttt ctccgtaatg gatcatttct
    33901 ttatagctgc aatttacttc cagaacgggg atgatgatcc cagattcttc gattttttta
    33961 tagggaaacc ctagatgtac caataaggct acccgagcct cttcaaacca gcggatataa
    34021 ttgctgtgat gaataatccc catttgatct gtttcataat aaaaaggttc tcgcacataa
    34081 cctgtaaatt tctccatgac tcatgcctcg ttttccattg attttttaca aaaataattc
    34141 atccatttcg gaagtgcttc gctgatttgt gtcggcaaaa caatatattg ttcttttgcc
    34201 agctggtctc caatgaaact atgaagataa atagctgctg caatcgtttg gtcattttga
    34261 ggaaactggg ctaaaaatcc tgtgatcatc ccagctaaag tgtcacccat gccgcccgtt
    34321 gccatagcag cagaacctaa aggattggtg tagaagtgct ggtgaccata aatttttgtc
    34381 tcatgacttt tcaaaacgag cacagcaccg atttcctgtt gcttcttttt gttttcttta
    34441 tcccgttgtt cttgtatcgg tagagaactc aaccgttgcc actccatttg atgaggtgta
    34501 agtacagttt gttcgggata gggtagcaaa agatgattag cagcaagtaa acttaaagca
    34561 gagccatcaa tcaccagcca ttgttctttt gattggtttt ctagtacttg tttgaaacgt
    34621 tccaaactct tagaagaagt acccaatccg ggaccaatca agagtacatc tgcttgcttt
    34681 attacggaaa gaaactggtc agtatccgtc caaactacaa tcattgcttc tggcagatga
    34741 ctgtgaagag cactccgatt tttttcatca gttactacag tcgtgagacc cgctccgctg
    34801 tgtacggcag ctaaagcact cataatgatt gctccgccat attgttcatt gccgccaatc
    34861 aaaacgatac gtccaaatgt tcctttatgt gaaatttttg gacgaggtgt gatgactctt
    34921 ttcactagtt gttcgtttat cagcatctaa aaacctcctt ttactcatta tagcgtatat
    34981 tttttttaga gaaaaacaga taagaaaagg aattgtcaga ttttttgaac tatttttttc
    35041 tgtaatttgt ggtatcatga acacgaagac ttaaataagt ggaggattaa ctttatgaca
    35101 attgattggc aaaaagaagt agaagcacgt aaagaagact tgcttgaaga cttgaaaaat
    35161 cttttacgtg tcaacagcga gcgcgatgac tcaaaagtaa caccagatgc accatttgga
    35221 cctggacctc gcgacgcatt gaaacatatg ttagcttatg gcgaacgtga tggatttaaa
    35281 gtaaaaaatg tcgataacta tgcagggcat attgatcttg gcgaaggaga cgaaacgcta
    35341 gggatatttg gacacatgga tgtcgttcct gcaggagatg gctgggacac agatccttat
    35401 gaaccagtaa tcaaggatgg aaagatcttt gcacgcggat caagcgatga caaaggtcca
    35461 agtatggcag cttactatgc gatgaagatc attaaagaac ttgacttgaa actatcaaaa
    35521 aaagttcgct tcgtagtcgg cagcgatgaa gaaagcggct ggggcgatat ggcttattat
    35581 ttcgaacatg aagaagaacc agattttggt ttctcaccag atgctgaatt cccaatcatc
    35641 aatggtgaaa aaggaaatgt ttctttagct ttacgcttca aaggcgacaa tgcaggagat
    35701 tatgtcttga aatcttttgt ttccggactt cgcgaaaata tggtgccagg aacagcaact
    35761 gcagcacttc aagtaccaag tgcagatgca gctatcgcga tggaagaagc attttaccaa
    35821 ttcatcgaag caaatccagt aagcggaacg attgaagcgg acaatacgta tgtaaaaatc
    35881 gaattagttg gtaaaggcgc acatggtgca agcccacaat ctggtatcaa tgcaggttct
    35941 ttcttggcat tgttcttaga taactatgaa ttcctaggat ctgctaaaca atttatccat
    36001 gtagcagctg cttatgtcca tgaagatttt tatggagaaa aactaggagt cgcttatgaa
    36061 gatgaaaaaa tgggcaaatt gacaatgaat gctggactat ttgcttttga agaaaatggt
    36121 acagaggaag cgaacttcat caatatgaat ttccgttttc caaaaggtgt aacagtagac
    36181 ggattgcaaa gtgacatcga acaaacagtg gggcaagaag gcgcaacagt tactcgaggt
    36241 gctcgcgtaa tggaaccaca ttacgtacca atggatgatc cattggtggc tacattattg
    36301 caagtctatg aagaccacac tggagaaaaa ggctacgaac aaatcatcgg tggcggaact
    36361 tacggacgtt tgttgaaacg tggcgttgct tatggagcga tgttcccagg atatactgat
    36421 acaatgcatc aagcaaacga atttatgagt cttgatgact tgttcagagc aacagcaatc
    36481 tatgcagatg ccatctatcg tttggcgaaa taattaatta aaaagaggat caagaattgc
    36541 aaattatttt tgcagtcttg atcctttttt tactttctac tgtggtattc atgataaaca
    36601 tgatatagat tcaattcata ttctttctta gcgttcgtga cgactgtatc caaaatcaac
    36661 cctgttacta atgaaagaag tgctaaagtc atcaatccag tacttaaaat cgcagaaggg
    36721 atgcgtgtaa taaagcttgt cagcatatat tcgcgaatca caggaatccc cgtgaccaat
    36781 ccaaataaaa agaagaagat ggtccaaatc ccaaagaaaa gcaaaggctt ataatcttta
    36841 aacattttaa taatcatcat gatcactttg aatccgtcag agaatgtatt caattttgat
    36901 tcgctgcctt caggacgatc acgataatcg attgggattt caacgaactt gaatttttta
    36961 tctaaagcat ggatcgtcaa ttccgtttcg atttgaaaac cagaactcat aataggaaaa
    37021 cttttgacaa agatacgatt gaatcctcga tagccggtca tgacatcaag aatttttgtt
    37081 ttatacattc tagtaattgt atttctaaca aggttatttc caaaatcgtg gaaaggtctc
    37141 ttattctcat caaagtaggt tccatttgat aggcgatcac caatgaccat atctgcttca
    37201 ccagaacgta atttctcgag caaaccatgt acagcttctg caggataagt atcatcgcca
    37261 tctaccatta aataatagtc ggcatcgata tcaaagaaca tttggcggat cacatttcct
    37321 tttccttgac gaggttcttt ttttacgatt gctccacctt gaacggccaa ttcgtatgtt
    37381 cgatcggtcg aattattatc atagacataa atatctgctt ctggtaattc tcgtttaaag
    37441 tctgcaatga ctgttgaaat tgttgcttcc tcattgtaac aaggaatcag aacagcaatt
    37501 ttttcttgtt gttccatttt tcactctcct taactataga aaacaaaagc taaattaaaa
    37561 atacctcata atcaaaggaa atgctagttt ttcccgctaa atatcattta tttaaaaaga
    37621 aaatactaga atatatgttt ggaaagctct atctaaaaaa ccacccacta caaaagaagg
    37681 acatgatacc tctcttttga atagtgggtg cgtattcgtt ctctcacttt tttgacgtat
    37741 taatctgttg cacctttttt tcttgaaatt tctaatacgg ttcctgtatg agcatctgct
    37801 aaaaattcat aataaacagt ttcgccatct tctattcgag aaagacctcc ccgataagca
    37861 ctcgaccgaa cggcgaattt cctaataggt tccttttcaa aagagatcca agaaccttcg
    37921 attgcacctt cttttagaaa agatgacttg atattttcaa gtacttggtc tgctgacaat
    37981 acttttttgc gatgatacca aatcgttgaa gctaccccgc tcaataatcc tagaccgatt
    38041 ccaactgcta acccgccttt aaaatagctt agttcgcttt cttcatacat gtggacatac
    38101 ctccttcttc tcacttgtta ctccttgtgc ttattttacc acactctcat attttctttg
    38161 aaataaataa ccgctaccag aaaaaataag ctataataaa tacgatcggt ctgcagaaaa
    38221 aatcatattt tatccgcaga tcaatataag tctgagagga gtttagtctg aaatttagac
    38281 gaatagcata tggaagaaaa aacatttcaa cgaatcaaag agttaacaga attacaagga
    38341 acaagcggtt ttgaacaaga tgttcgcgct tatatggaaa gtcatatgga accgctggta
    38401 gatgagttac agttagatgg tttgggcggc atttttggtc tgcgccatca tgaagaagcg
    38461 gatgcacccc gtgtaatggt cgcagcacat atggatgaag tcggattcat gctgacacaa
    38521 atccaagaaa acgggctttt taaagtcgta cctctaggcg gctggaatcc ttacgttgtt
    38581 tctgctcagc gttttacact taaaacctct actggtaaga attatccatg tatttcttct
    38641 tccgttcccc cacatttatt gcgaggaaca agcggacaaa aatcagtaga agtaaccgat
    38701 attttgttcg atgctgggtt tgaatcaaga gaagaggcaa tgagttacgg tgtccttcca
    38761 ggagacacaa tcgttcctta cgctgaaacg atcaaaacag ccaacggaaa gaatattatc
    38821 agtaaatcat gggacaaccg ctatggctgt acaatggtat tagaagcttt agaagcgttg
    38881 caaaatgaaa cattaggtca tacattgatt gcaggcgcga atgtccagga agaagtaggc
    38941 ttgcgtggtt ctaaagcttc tgtcaataaa ttcaagccag atctgttctt tgcagtagac
    39001 tgctctgcag cagatgatac agtgacgaaa aatggcacat tcggacattt aggagaaggt
    39061 acattgatgc gtatccaaga tccaggtttg atcatgcttc ctcgtttgcg cgagtacttg
    39121 ctggatatcg ctgaaacaaa caatatccct tatcaatact ttgtttcaaa aggcggtaca
    39181 gatgcaggtg ctgctcatac acaaaacgaa ggaatcccaa gtacagtgat tggcgtagtg
    39241 ggacgttata tccatacgca tcaaacgatg ttcagtattc gcgattttga agctgcacga
    39301 gaaatgttga ttcaaacact gaaaggtttg gataaatcaa cagtaaatac gatcgtatac
    39361 ggaaaataat gcgatagtga aactataaaa tctggcaaaa gattgctttt tgccagattt
    39421 tttttataaa aagaggatca atgtttgtta gttcgaaaga aaggtgtata atttaattga
    39481 aaaatacata aacagaaagt agaggacaaa gaatgattat cccaaatagt ttagaagaat
    39541 tagcaggcta cgtagaaact ggaaagagtg tatttttctt tactgccggc tggtgcggag
    39601 actgccgttt tatcaaaccg caaatgcctg aaattgaaaa tgacttttct gattggcaat
    39661 ttattgaagt agaccgtgac aagtacatag atgtagccgc tgaatggaac atttttggta
    39721 tcccaagttt tgtcgtgatc caagacggaa aagaattagg tcgtctagtg aataaagatc
    39781 gtaaaacaaa acaggaaatc gaatcgttcc tagaaagtgt aacaaaaggc tgacatctgt
    39841 taggaggggt caagatgaat caagaatata agaatatttt agtaggtatc gacggtagtg
    39901 aacaagcact tgctgctttc aaaaaagcgg tagaagtggc tcgccgtaat aatgggaaag
    39961 tatatgtagc aaacgtaatc gatcagcaat tttataactt tatgggctat gcacctattg
    40021 atcaaaatct agtcgatcag caaactgaaa cagccaagga aatgatcgaa gaatgtaaag
    40081 aatatgggaa atctgttgat tacacaaata tagaaggaat tattgcatat ggttctgttc
    40141 gtgaatccat ggcagtcaaa ttgccaaaaa aatatgagat cgatctaatc atggttgggc
    40201 agtcaggtct aaatgcagtc gaacgcttta tgacaggaag tgtagccagc tacgtgatac
    40261 gtcattcacc ttgtgatgtc ttgatcgttt ctgacgaaga agaacagcca agtaattaag
    40321 caagtggaac ttgtacgacc tttttaatca attaactaaa acagtaaagg gactgtgaca
    40381 aaagtcttgt cacagtccct tgttacgaat aaatggtgtt ccagaagagg cttcttcgga
    40441 aataaagcat gaaaaaaatt tcatgcagtg aatgcaattt tttgttaaga taaatctcag
    40501 caaaggataa tggaggaaag aacatgattt ttgcttataa caaagcacac gtcggagaca
    40561 ccttgatggt tattatagcc gacgataaag gaacagaaaa cactgttgaa cgcaaatgga
    40621 atgttgctcg gatcaaagat gaacaaggtc aagttgttgc ttggaacttt ttccatatct
    40681 ccgatcatct aacaatcgaa gggaacggcc aagtaagctt aacagatgag gaattagcga
    40741 aattgaatcg attgatcaaa gaagcaggat tcgaagaaca gcttgaagct gatcatgaac
    40801 cgaagatcgt tgtcgggtac gtaaaaacat gcgtcccaca tcctgattct gaccatttat
    40861 cgattactga gacagaagta gacaatggta cagtattgca aatcgtttgc ggtgcaccca
    40921 atatcgaagc tggacaaaaa gttgtcgttg caaaaccagg agctatgatg ccagatggaa
    40981 tgatgatctg gccaggaact ttgcgagggg tagatagcta tggaatgatc tgttccgctc
    41041 gtgaattgca tttgccaaat gctccagcaa aaaaaggaat cttagtattg cctgaggatg
    41101 ccgtagtcgg agaagcattt ccaaaataaa gaggttgtga caagtctttg tcacaacctc
    41161 ttttttatta attttcacta ttttgatttt gttttaatgc cgattgatcg atcgtcaatt
    41221 caacagtagt tgttttttct ttcgagcctt catagtaagt gattttgaca gaatctccca
    41281 cttttttctt atacaaagca gattgaagtt ctacaccaga agaaacttct ttgtcatcga
    41341 tcttagtaat gacgtcgtat tgtttcaatc cagctttttc agccggtgta gctgtttgga
    41401 catttgtcac gatgacaccg ttagttacag atgaaggaat cttcaagatt tgttcttgtt
    41461 gctgagtaga aacggcagat aagtcgacca ttgtgatacc tagagctgga cgtgtcactt
    41521 ttccgtcttt ttccagttga ttgatgatat tcactacatc gttacttgga atagcaaatc
    41581 ccattccttc tacgcttaca ttagaagtag attcagaagt acttgcgatc ttacttgagt
    41641 tgataccgat aacttgtcct tcgatattaa ctaaaggacc accagaattt ccagggttga
    41701 ttgctgcatc tgtttggatc gcattgatat tgactgtttc gttcgattca ttcgtacttg
    41761 tcacttgtcg gttcaaggaa gaaatgattc ctgaagtgac agaatttgca tattcagatc
    41821 ctagtgggga accaatcgca atcgcaggct caccaacctt cagagcactt gagtcaccaa
    41881 aagaggcaac tgtttcgact ttgtctgaat tgatcttcaa caccgctaaa tctgaataag
    41941 catctgttcc aactaattca gcttttacct tcgtaccgtc tttcatcaag acttcaagac
    42001 cttgctggcc atctactacg tgattattcg tgactacata agcagtattc ccgtcttttt
    42061 tgtagatcac gccgcttcct tcactataag cttccaaatt actatcatct gagctgctac
    42121 tttcttcttg ctgcccgaat agtccgccaa aaccgcttga ttggttttga ctttgtaagt
    42181 tgataacaga aacgacagcg tcttgaactt tatctaccgc tttggtgata tcagaatcta
    42241 cattgacttt gacattttcc acaactgttt caccggcaga attctgattg ccgcttgtag
    42301 cagtagaact attgttgcca gtgcccatcg ctgcataaaa aatgccagct gtcagtagtc
    42361 ctccaacgat ccctccaaca agccccagac ctaacttacg ccagaggcta ttatttttac
    42421 tttttttcat ctttggtgtc acattttttc tatccatgtg ttcctcctcc aattacattc
    42481 atttatctac tgttagtata gtcttatttg ttgactttag tctactttga gactgtgaac
    42541 tttttatgaa aaactttagg aaaaatcatt gttttcagat gaaaatattt gattttcttt
    42601 ttccacagat actgtgtaaa actacgcgga taggattgga aaaataaggg ataaaacata
    42661 tccacagcct gtgtataaag tggataaatt cttataaaac gtatgtttgc ctaatgaatg
    42721 tgatgaaata aggtaaaata acactgtttt ctgtggataa acctgtggat gttgtgcata
    42781 actgttgggg aaatcttttt aacaaaaata gccaaaataa acatatagac gaaaaaattg
    42841 attataataa acaaagtgag catgaagacc aaaggagaga aaacatgaat atcaaaatta
    42901 tctcagtagg aaaattaaaa gaaaaatacc tgatccaagg tatcaatgag tatgtcaaac
    42961 ggctgaatgc ctatgcaaaa atcgaattga tcgaagttcc tgacgaaaaa gccccggaga
    43021 atttaagcga agcgcaaatg agacaagtaa aagaaaaaga aggggaacgg atcttagcga
    43081 aaatcaaaga acaagaatac gtctatgcct tagcaattga agggaaaaat cctacaagtg
    43141 aatcttttgc taaacaaatc gaccaattag gtatccaagg aaaaagccac cttgtttttg
    43201 ttatcggcgg ctctttagga ctcagcgaag ccgtcatgaa aagaagcaat gctcagatct
    43261 cttttggaaa aatgacgtat ccacatcaat tgatgcgtct gatccttgtc gagcaaatct
    43321 accgtgcctt ccggatcaat gcgggggctc cataccacaa ataatgatat tttttaagag
    43381 aaaaaattat ccctatttta aataatagca ataggtagtg gtagggagaa atttataaat
    43441 aactttttta tttgctttgg tagccaagta agttgcaaaa gaaaaataga tactgttaaa
    43501 aagataatgt agacatagaa acaaatacgt aaaattttag ttagaataat ggcaaggaga
    43561 aaaaacatga tagatgaaaa aaagtgttta aaaaatctat tagaggatat ggatgatatt
    43621 gaagaaaact atctaaatgg actgggaaaa caaatatatt tcagatactt aagctatcta
    43681 aaaatgaaat aagacatagt aattttttag cttttttgtt tgctccaaat gaaacccaca
    43741 atttatctga tgagtttttt aaaatgtttt tgaaaaggta tatagattct aatgatgata
    43801 ccaaagctgc tatcaattat tttgatgctt tacttaatag ctatgaagat ttaattgttt
    43861 acagagaaaa taagaatatt gatattttat tggtatcgga aaaaaataaa atagtggtgt
    43921 gcatcgaaaa taaaattctg tcttcggagt ctggagggca acttaataaa taccaaatat
    43981 atatagaacg caattattct aactataaaa aaatttttgt ttttttaact ccagatggaa
    44041 atgaacccac aaatcctagt tggggcataa ttacttacaa gaatatcgtt ga
//
LOCUS       DACCXO010000013        42634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.013, whole genome shotgun sequence.
ACCESSION   DACCXO010000013 DACCXO010000000
VERSION     DACCXO010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.013"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            161..499
                     /locus_tag="IVY70_000647"
     CDS             161..499
                     /locus_tag="IVY70_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957805.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            609..830
                     /locus_tag="IVY70_000648"
     CDS             609..830
                     /locus_tag="IVY70_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957806.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            850..3006
                     /locus_tag="IVY70_000649"
     CDS             850..3006
                     /locus_tag="IVY70_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957807.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            3018..3251
                     /locus_tag="IVY70_000650"
     CDS             3018..3251
                     /locus_tag="IVY70_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7957808.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            3241..4107
                     /locus_tag="IVY70_000651"
     CDS             3241..4107
                     /locus_tag="IVY70_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957809.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            4122..6092
                     /locus_tag="IVY70_000652"
     CDS             4122..6092
                     /locus_tag="IVY70_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957810.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            6259..6453
                     /locus_tag="IVY70_000653"
     CDS             6259..6453
                     /locus_tag="IVY70_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957811.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFKTIS"
     gene            6487..6822
                     /locus_tag="IVY70_000654"
     CDS             6487..6822
                     /locus_tag="IVY70_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957812.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
     gene            complement(7079..8662)
                     /locus_tag="IVY70_000655"
     CDS             complement(7079..8662)
                     /locus_tag="IVY70_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957813.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRDRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDKELKKLYELAESKSDMYLDIIDNIINKSEDY
                     LRKEINSEKELLNYVHKIK"
     gene            8784..9368
                     /locus_tag="IVY70_000656"
     CDS             8784..9368
                     /locus_tag="IVY70_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957814.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            9379..9624
                     /locus_tag="IVY70_000657"
     CDS             9379..9624
                     /locus_tag="IVY70_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957815.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            9630..10352
                     /locus_tag="IVY70_000658"
     CDS             9630..10352
                     /locus_tag="IVY70_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957816.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            10483..11226
                     /locus_tag="IVY70_000659"
     CDS             10483..11226
                     /locus_tag="IVY70_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957817.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            11232..11597
                     /locus_tag="IVY70_000660"
     CDS             11232..11597
                     /locus_tag="IVY70_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957818.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            11613..12143
                     /locus_tag="IVY70_000661"
     CDS             11613..12143
                     /locus_tag="IVY70_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957819.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            12305..12613
                     /locus_tag="IVY70_000662"
     CDS             12305..12613
                     /locus_tag="IVY70_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957820.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIERCH
                     YGQHFWTGEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
     gene            12609..13028
                     /locus_tag="IVY70_000663"
                     /pseudo
     CDS             12609..13028
                     /locus_tag="IVY70_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352869.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13165..13965
                     /locus_tag="IVY70_000664"
     CDS             13165..13965
                     /locus_tag="IVY70_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957821.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            14002..14184
                     /locus_tag="IVY70_000665"
     CDS             14002..14184
                     /locus_tag="IVY70_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957822.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            14295..14612
                     /locus_tag="IVY70_000666"
     CDS             14295..14612
                     /locus_tag="IVY70_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957823.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            14615..14847
                     /locus_tag="IVY70_000667"
                     /pseudo
     CDS             14615..14847
                     /locus_tag="IVY70_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14962..15441)
                     /locus_tag="IVY70_000668"
     CDS             complement(14962..15441)
                     /locus_tag="IVY70_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957824.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            15561..16898
                     /locus_tag="IVY70_000669"
     CDS             15561..16898
                     /locus_tag="IVY70_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP7957825.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            16921..17448
                     /locus_tag="IVY70_000670"
     CDS             16921..17448
                     /locus_tag="IVY70_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957826.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            17435..17998
                     /locus_tag="IVY70_000671"
     CDS             17435..17998
                     /locus_tag="IVY70_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957827.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            18420..19157
                     /locus_tag="IVY70_000672"
     CDS             18420..19157
                     /locus_tag="IVY70_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957828.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(19174..19590)
                     /locus_tag="IVY70_000673"
     CDS             complement(19174..19590)
                     /locus_tag="IVY70_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957829.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKYKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            19982..21229
                     /locus_tag="IVY70_000674"
     CDS             19982..21229
                     /locus_tag="IVY70_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957830.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            21497..23887
                     /locus_tag="IVY70_000675"
     CDS             21497..23887
                     /locus_tag="IVY70_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAP7957831.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            23936..25771
                     /locus_tag="IVY70_000676"
     CDS             23936..25771
                     /locus_tag="IVY70_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7957832.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKSVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            25846..26703
                     /locus_tag="IVY70_000677"
     CDS             25846..26703
                     /locus_tag="IVY70_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7957833.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            26693..27802
                     /locus_tag="IVY70_000678"
     CDS             26693..27802
                     /locus_tag="IVY70_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7957834.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKTETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVIGALASLKVLILLIKEFRKA"
     gene            28141..28941
                     /locus_tag="IVY70_000679"
     CDS             28141..28941
                     /locus_tag="IVY70_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957835.1"
                     /translation="MVGVSKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            29029..29835
                     /locus_tag="IVY70_000680"
     CDS             29029..29835
                     /locus_tag="IVY70_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957836.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            complement(29852..31279)
                     /locus_tag="IVY70_000681"
     CDS             complement(29852..31279)
                     /locus_tag="IVY70_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HAP7957837.1"
                     /translation="MMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFDFIYPIV
                     ESTYSTYGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYSFEDPIP
                     HFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANANKHKFT
                     KEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESGYYVKGE
                     REKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRVVADAGY
                     KTPKFVHFLSHLKLYPILPYTAPHGVKGMFRKKDFVYDAYFDCYLCPNNQPLLFSTIT
                     RDGYRTYKSNPKFCVSCPLLENCTLSTKHQKIVTRHIWADLMDELEHQRHTALNREIY
                     KKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLWKTKKPM
                     AFYFKIMQRLTKRSGLITNSFLSTI"
     gene            31600..32469
                     /locus_tag="IVY70_000682"
     CDS             31600..32469
                     /locus_tag="IVY70_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP7957838.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            32489..32836
                     /locus_tag="IVY70_000683"
     CDS             32489..32836
                     /locus_tag="IVY70_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957839.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            32849..34111
                     /locus_tag="IVY70_000684"
     CDS             32849..34111
                     /locus_tag="IVY70_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957840.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            34191..35567
                     /locus_tag="IVY70_000685"
     CDS             34191..35567
                     /locus_tag="IVY70_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP7957841.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKVEQGAKTLEKLQAEKLEKVLRALGK
                     ADPEPQTSNNTNNKQPIASKTKPKANDPKGSENVVITTDKSEKKQINGSTSPIMQKML
                     KAKKQVHK"
     gene            35564..36193
                     /locus_tag="IVY70_000686"
     CDS             35564..36193
                     /locus_tag="IVY70_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957842.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            36212..36691
                     /locus_tag="IVY70_000687"
     CDS             36212..36691
                     /locus_tag="IVY70_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957843.1"
                     /translation="MLELFEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            37193..37246
                     /locus_tag="IVY70_000688"
                     /pseudo
     CDS             37193..37246
                     /locus_tag="IVY70_000688"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            37250..37849
                     /locus_tag="IVY70_000689"
     CDS             37250..37849
                     /locus_tag="IVY70_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP7957844.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            37865..38341
                     /locus_tag="IVY70_000690"
     CDS             37865..38341
                     /locus_tag="IVY70_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957845.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            38358..39260
                     /locus_tag="IVY70_000691"
     CDS             38358..39260
                     /locus_tag="IVY70_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957846.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            39333..39524
                     /locus_tag="IVY70_000692"
     CDS             39333..39524
                     /locus_tag="IVY70_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957847.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            39688..40275
                     /locus_tag="IVY70_000693"
     CDS             39688..40275
                     /locus_tag="IVY70_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957848.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            40275..41174
                     /locus_tag="IVY70_000694"
     CDS             40275..41174
                     /locus_tag="IVY70_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAP7957849.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            41174..42088
                     /locus_tag="IVY70_000695"
     CDS             41174..42088
                     /locus_tag="IVY70_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957850.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            42140..42355
                     /locus_tag="IVY70_000696"
     CDS             42140..42355
                     /locus_tag="IVY70_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957851.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
BASE COUNT        15973 a         5885 c         8341 g        12435 t
ORIGIN      
        1 gaaaatctct tttaatcatc gaaaatttat gttattatga taacataaag atgtaaatat
       61 cgagacgtct ttttcttagt tagatcattg acaagtgaac aaataagatt tgtcgatgat
      121 ataattaact tattaaaaat attaaaaagg aggtttttat atgttcgatt ctattaaaaa
      181 tttggttagc ggaatgacgc ctgatatgaa aactttagta caagttgttt tgtatttctt
      241 ggcattcgtt ttttcgttga aaccaggatt cactgcaatg aaattttttg gtgatgcaaa
      301 atggggagat ggattaaaat atgttggggc gattattgca atctttatta ttcctgtaat
      361 tacaaccgtt atgctttaca caatgggtac taaaacaggt aatgatttga accaaaaagc
      421 taatatcata tcagcactta ttccagttgt tggtatctat ctagtttcaa aatttccaaa
      481 aaatgaactg ttaggttaaa agtgaattga acaaatttgg ataatatgcc gatttgttca
      541 atttactttt taccaaaaaa ctacgctcca agcgtagttt agaagtgagg gaagaaagta
      601 aatgtaagat ggtttttgat agagaaaaaa tgttagctca cgccaacgaa gtattaatga
      661 gcagtctgaa aggtaccgaa ctggcaaaga taatgaatat gaatgttaac cagttctatg
      721 attatcgaaa tgggagtaaa aagattgaaa aagccagact tgaaacgctt attaaattcg
      781 aaaaagcata tgtatatatg ctagataaac agaaaagaac tattgactga aaaaaggagt
      841 tttacaataa tggcaaaatt caaagatatt accgaatcta ttaattatgt ggcagaaaca
      901 gttcctgtag ttgcactctc tttgaagcca caattactcg aagtgtacaa aaaagaagaa
      961 aaagaagcga aggcaaaaaa agctaaagca aaggtatgga aagaattcga tgcgttgctg
     1021 accaaagatt taccaaagaa aaattatatg gtaattgaag atgtacaaaa gctcaacagt
     1081 gagggagtta tagatgagga tgatgctatt ctcgattctg agaagtatgt cgttctattg
     1141 aagtcaagtt tgaattatgt agctgatcta ccagaaaaat ttaaagagaa gctattagca
     1201 ggtgtccgtt ttatggatag tagtggtcaa ttattagtta ttccggatga tcctaatgtg
     1261 tttgaatcag aagttgaaga agctacagac ttctatagcg cagtagagca attagaagta
     1321 gctactaatg gattagaacg aattgctgag accttcaact cttctgaaga agttgaattt
     1381 gaagccgtag cagaaaatgt accagatgaa aaagaagctc tttttgtgga tgataacaaa
     1441 ccaaccgaag cagaaaacca agaagtaagc atagaagaaa cacaaataca aacagaaacc
     1501 taccaacaac caaaaattga tgaaaaaccc gaatcccaaa gtgacaaaga aaattatgtt
     1561 tatgaagctg aaaaaatcac atctgaaagt gatgtaccga aaaacttaga tgctgatttt
     1621 ttagaaaaaa attttgaaca gatcattgct gacaaatttc aacctgtagt tttagatgag
     1681 attaatgacc ctgtcgattt aaacgaggag atgctatcca tacctgagtt gaaaccattg
     1741 ctgtttttag ctaaagagag tatgaacgct cgcatctcta ttgctaataa taaaatcaac
     1801 agtgaattaa ataagacgaa ggagcaattg atcctcaaag gaaagcaaat gttagctgat
     1861 gagcttttgc ggatagagga agcaacctca ttaaatggtc aaaatcagtt tacaaataca
     1921 ctatcggttg ctaaagagag ctacgaagca gaagcatcta aaattgatga tttagtgatg
     1981 gaaaaatcca atatcattcg taaagattat gatatggcga aacaggctta tttggatgct
     2041 gaactaaaga aactagagca acggtatgat gctgagcatt taccagaagt taaacaacaa
     2101 gaagatgaat atagaaaagc aactgaagaa agtcttgatc aacagtacca agaagcaatt
     2161 gaatctatta aaaacgatgc attgaaacaa agccaagaag caaaagaagt tgtggtggaa
     2221 cgaattatca attcattgaa accagaaatt gaaaaatcat taacagacgt gcaagaaaaa
     2281 actttagaga ttgtcgaaaa aacaaagtca gaaaatgaag ctgagttcca tgatacaaaa
     2341 gagaaaacaa cagatgtggt tgtcaaatat tttgcgaaca aagaagaagt tgaaaagcag
     2401 gtagctaaca acacgaaaga attagaaaca aaacttgtag agcaacaaaa aactatcgaa
     2461 gaatataaag atcgaatgaa agaggtcaaa gatttagcag agggttataa attaaatagc
     2521 gaaaagttgg cagaacaaag tgaagcttta agagaagact tatctcgttc ccgaagtgat
     2581 ctagctcaaa tggcagaaaa aacgaaatca ttagcagttg ctacagaaaa tcaatcaagt
     2641 tcaaaacgtg attttacaag taaaaagatg gctactatca tttctgtcgc tgcaatggct
     2701 tgcggaacga tcttgggatc gtcctacttt attagtcata ataatcaaca agcacaggct
     2761 caaactcaaa catctatttc gagagtggaa aattcgattg ggacaaatac gaactctgct
     2821 tcaacaaata gtagctatga agcaacacca actacagagt tcaaagttgg gaaagatgtt
     2881 gtcatcagta ttgatgggaa aacaactaag gctgaagtga tttctattga tggagaaaaa
     2941 gcagttgtga aaactccaga cggaaatcag tacaaaattc aagctcactt atctgataat
     3001 aactaaggag ggctattatg cgagaagaaa aaatagttta tccagtaatt caaaacaatc
     3061 aagaaaacat gacctcttct cagcacttac aggaagcaca gctcgtgtat caggtaaatg
     3121 aactaccaca aacgaacgag acaatgagca agtcaatatc tttaattggc ttgcttattg
     3181 ttgcaatagt gataactgta ggtgtttacc gtaagttgaa cgtgaaagga agtatttctg
     3241 atggcagata actcaaacta tggatttaaa cataacttac atcaaaaacc ttctgaaaca
     3301 attgtcagag ttgaatggct tgaaaagcat gtcaatagca cctattctgt tacagcaaaa
     3361 agattaattg tatcagctat agttgttgca gtcgttgttc ttggatcaat tgtttactta
     3421 gtttctgata cgattaaact gaaatttatt tttgttccaa cgttattaat gataattgga
     3481 tttcttattc taattagcca gtatatgttt attgatccat ctagtggttc attgatttct
     3541 ggttctatta gactgttcaa atcagtagtt aattcagcaa gagtatctat ggggaaaaag
     3601 aaaccatttc gaggcacaga gattttaaaa gtgaaacaag atggaacaat cgtatatcga
     3661 gatgggagtg tagcattaat gttcaatcta gatggggcta catcactaac cgcttttcca
     3721 gaagaagtta tggctcagga atcgaaatca atacaatatc aaaataccag agatcgttcc
     3781 ttagctgaaa tcaaaatcac ttcatctcaa cctcagaata cggaaagaca aactcgaaat
     3841 atgaaaatac aaaaacgaaa tgcaaggagt aacaaagcag tctttgacat aatagatcag
     3901 caagaacgat ttgtatcaga atatattgat ggtgtgaaaa caacagtaat ccaatatctt
     3961 atcttgaaat ctcaatctaa gatggtattg gatgaaggtg tggaacgttt agaaaactat
     4021 acgagtagag gactttatta tagtgtcact cagttgtcag ctaaacaaac aaaagaaata
     4081 ttatctgata tttatgcact gaaatagagg tgagagatta aatggaaatt atgggtagat
     4141 cacaactatt aaatttcaaa aaaaagagaa aaactaaaaa agaacaaaaa actagaaatt
     4201 caaaggtagt gaataatacc tttgttagtg atttaacatc tttagttggt tatcaattta
     4261 caccatctta cattaggaca ggtaagcggt ggggaacaat tgtgaaatta gtgaatactt
     4321 atggaatgaa tagaaattat ggatttggtt ggatggttaa agctattcct gaagtgagag
     4381 tagaaggggt aaaagcctat ctaattgaag cagataagcc agtgtcacaa aaagatcaac
     4441 aagaaataat gcacgagaat gttgggggat caacgaatag ttatgaaaat aatttagaag
     4501 gaaaaaattc agatgctgaa attagcaaaa ctgttatgcg gattatggat ttgaatacag
     4561 catctttact agacagcatg gcaaataaaa ttattgattg gaagctagaa atcctattag
     4621 tatccgattc tcctaataaa atcagcgaac agctaattaa actccaacgg ttgtatgatg
     4681 aaaaaatgta tggattaaag cttatgtcat atggaggaga tcaacgtgat ctgtttgata
     4741 gacttttaag tgaaccaatc gggaatgtct atgagtatac gtctatgagt tcggattttg
     4801 caggaaatga tcatgcaatt cgcaaaggtt tagatgatgc gaatggatgg gctatcggca
     4861 ctatgtctac tagctacgca agtggtgtag cttttatggc actggatgat tcttttacaa
     4921 gtaaagtttt agtggcatca cacaaaaacg ctacaattaa aggctatgat gaaaaaatct
     4981 cagcatcatc actttggggg caattgattg ctaataatgc aatggcaaat cataaacgaa
     5041 cttttcatat tgtcttaaat aaatttgatt actttggtga tccaactggt agaacatttg
     5101 ttgccaaacc ttcgttaaac agtgtaatag aaaaggttaa tttggctgaa gaaggtctta
     5161 actctttgga gctttttggt aatcaaaagg acattggtga tttatataga cggaatctcg
     5221 ataaactaac tcagattttt tatttgttag caggcagaac attagattct gcaactagag
     5281 ttttattgaa aaaagaatta ggtaactttt atataagtca gaaactttgg gatattaatt
     5341 atgaagaacg ttcaaaactt ttggtgaaca gtagtgatac aatgccagtt cttggggatt
     5401 ttattagcta tttgactaat gctgaaacaa aagccaaaaa atctggtact gaaaaagaag
     5461 tcgatcgtat tattgtatta aaagacacat tgagaacagc attagacagc ttctctagga
     5521 tgtttaatca aagaacgaca ctgaaagaaa tcggtaaaag tgacaaatta cagttttact
     5581 ataacttagg tgatctaaga gcagaaacca atgttatgga agctcaattt ttaaatacct
     5641 ttgatttcat cacatcacaa gcaaaaaaag atgatattgt tatgattcac ggcttcgata
     5701 agttatctat tgaaaccgtt gggctaatta aaaaaagatt aacaatgctt gaagaagatg
     5761 gaattcgtct tgcctttcta tttgatacaa tcggttcagg agaagacaat gaatacaaag
     5821 aagatattga aacagctgat atattcaata cagatggaaa aatttaccaa agttttgaaa
     5881 gtgattttaa ttacactatc attggaaaga tgaccagtgg agagttaatg aagtatgaaa
     5941 aaaaagttgc tcctgctaga aaaggagaag atgatcaaaa actttctgtt gatttgagac
     6001 agtatttgac aacaggaaga agtaataaat ttcagatcag gagaccagtg gatctgacta
     6061 ataattttgt tattggagag tttattcttt aagttttatc tttgattttg agtgtataag
     6121 gaaaggataa gaaaaaacat gtatatttga gactaaaaac agtcttagag aacatggtac
     6181 gttttgtgaa aagagaacta tagaaatttc ataaagtgaa accttactga gtgaaataat
     6241 tatagtggag gttaaataat gaaaccaaac ataaaaattt tagatcgaat atttctaggt
     6301 agagacacag aagtaatcct aattcaacat gaagaaggat ttgaagttag tataggtatt
     6361 cagaaacttc aaaaacctca ttattgtaat cagttgtata aaaactttac tgatgaagaa
     6421 aaagcgagag ttttttttaa aacaatttcc taggaaaaat agttaacata aaaggaatga
     6481 attgttatga aacaatattt aaatgaatgg aaagcgatag aggggtcatt agtaacgcaa
     6541 agaataaagg aattacctga ttgcttagag aaggatcatt tgtttcaaat acgagaaatg
     6601 ttgaaaaaag aacagtttga tccagaccaa tttttagtag tcgagtatcc aactattgga
     6661 gtgtactgtt gtaaccatat caatgatgga aaatatttta ttattcagga gtatggagga
     6721 cagttaactc ctttctatac tacatgggaa atgagtgaag aaggaatcaa taattttcca
     6781 tgtgaaagca tcgaagattc aatttcacac accgaatgtt aattgattat aactataaaa
     6841 aaggattgaa cattatgttc aatccttttt tcaatgaaat gttttccgcc atttcattgt
     6901 ttggtgcgat ttctatttta ttatatcaaa ataaagagag attctaaaga aagaagaggg
     6961 aaatatataa atcaagcgac agtatattta ttattaaatg ctattgtgag aatataacta
     7021 attagtttaa tttgaaagta aatatacgct ataaaagtgc atcttgtgtt ataaaaaatt
     7081 attttatttt gtgaacataa tttaacaatt ctttctcgga attgatttct tttcttaaat
     7141 agtcttcaga cttattaata atattatcta ttatgtctag atacatatca cttttcgatt
     7201 cagctaattc atacagtttt tttaattctt tatcctttaa ttgaaaaccc tgatccatag
     7261 tcactgcatt tggatataga agcccactat aatgtttacg taaattcact tggaatttct
     7321 gattataaaa gtctaacaga ttagaaataa aaggcggaaa attactgttt ttgtaaaatt
     7381 gattatttcc atttaggttg tatagtaatt tgttaaattg ctcctgattt aatttattgt
     7441 aaatccatgg taatacttcc atatccttat ctgctaacct gaatgaacta gcatcggtaa
     7501 gatgttcgat cataaaatct atgaacatct ctttttcacc aaaatataaa tattgattat
     7561 atattgtttc taatgttgaa gatggtagcg aagaaactct cattttttgc tggagtatag
     7621 gatcaactag actatttaag tgattttgta attcttcatt gttgtttcct agaactgcaa
     7681 gggaacgtaa acggtagtaa tattctattt ctgttagtac ttgttgtttc ggtttaaaat
     7741 catttattga tatttgtaaa ttttgaattg tctcgtctga aaaatcatgt tgtaattctg
     7801 gatgggtagc tataaaagta agtaaaaaga acccttttgt atacgtgatc tgttgatttt
     7861 caccaagtga tttattaatt tcctctcttg attcagctgt gaacctatac tttgcaattt
     7921 ctttttttag aatatttaaa tagaattctt tattatcaaa aattagggca ttaagtagtc
     7981 tgaaagtact ttccctatct ctatctgctt cctcatttcc ataagataat tgaaaaacaa
     8041 acttaaaggc tgtcttacat aggttttctt tggctttttc gcttagttta tttagatagc
     8101 tattttttag tgcagagtga aattcttcag gtgtaacaga catagcatgt atgcgacttg
     8161 catattgtat aatttcgatc gttacttttt ttcctaatat tggatttttt aggaatataa
     8221 tttcatagga cttccgtata acatcctgac aagtttcttt tgttatatct cttaattccc
     8281 aagtattacc gttgtaagtg actacaggat gagctgaata atttctttga tccttaatat
     8341 attttaaatc ctcatattca tagctatcaa ttagctcagt tgaactataa gctttttcta
     8401 gtagttccat ttcccatttt ggagagtttg gatcactttt tcttagtcta gctatatctt
     8461 ctaaaatttt cttagaagta ttatcctcgt aaatatttgc taaatcagat aatttatcaa
     8521 ttaaatctaa aataagcgca ctgtaattga cagctattgc agaacgataa tttccctgat
     8581 aaaaactact tctaacttct tctaataatg gaataattct aggattttta acttttttta
     8641 tttcatcatc taatataaac atataaatct ccttttttta tttgtatgac aagtttacca
     8701 taaaaaaaaa ttaaaaagcg agacatattt atgttgttgt gttatcataa taacataaat
     8761 atgtagagtg agatgatgaa aaaatggatt tgaaacaatc gttttacatc tggttatatc
     8821 ctgataatcc tatgtcaata atggctgata tttttgtagt tagcatctta ttgtatttat
     8881 tttatctaat tttctttcac aatttaatcg ggtacatgat tcttgattgg aaaggaatgt
     8941 tcgatataga aagaaaacca tttgctaaca atcaaaccta taccgtcttc gaaagtggag
     9001 attgggaaga aaatgaagtt gatgtgatcc atgaaattgg attagcttta tcaggaatac
     9061 aagatgctga tgttttggta tcaatgacaa cggatactgg atacaacgct tatgaggtat
     9121 tttatcttat gtattttata tggcgtgatg gtcaagaaat tatcaatggt cgtgctgaaa
     9181 aaacaatcaa ttctaatact gaaatttctt tttgtatggg tgaaggggaa atgattgtat
     9241 ataaagatgg gtcagcaaac attaaaacag cctttggtga tattgaaaca agtattaagg
     9301 aacttaggga atatgtgcag aacccatata attttcaata tttagcagga agaataaatt
     9361 tagtttagga gtaaaaatat gaaaaaaatt ataaatcaac caacaaatca cttgataatt
     9421 gaaggtgtta ttttcgtaac aatgtatttt actgtaggaa atggcatctc aaatacaatc
     9481 attcaaaaaa atccacaata cctattattt agtctttatg cttggattgt acaaatggtt
     9541 tgggtttcca tattgatgct gttgttttat tggatagtac gggttattct tttattgtta
     9601 aacaaaagaa aaaaggatag gtgaataaaa tgaaatcaac taaattgaag gttgtattgt
     9661 tatcgacagc agcattaata acccttacag catgtggtac aactaatagt gctaataata
     9721 acaaagacac aaagtctagt agtacgatgg ttactaaaaa cagcactaca tcggcatcaa
     9781 caacaatgtc ttcaatgact gaaaataatg ctgaaaagta tcaaaaatac gaagacatta
     9841 ataaaggtca aaagccggat gaagtttata acaactttat taatacagta aaaaatgatg
     9901 aaaattatgg gaatgatcca ttgcaaaata aaaatctgac tatcgttctt tacaaacctg
     9961 attgtccaga ttgtcaaaaa gcagaatcaa aaatcatgca agaaataaaa gaaggattta
    10021 gttatgctcc aacattaatg catgatatta ccataaataa accagcttac gagtattacg
    10081 cagttgctgt agatattact gatggtgtac ctgagtgggt acaaaacatt cctggtctgg
    10141 ctgatgagaa caatcaatat catacgccaa cagttgcagt agtaacacca aagaaagtta
    10201 acggcacact atactgggat gtaatgggaa tgacaacaaa tacaactgat gaaatgattg
    10261 aaagtacaac taaatattat atggatttgg gagcatataa attatatcaa caatcagagc
    10321 ctgttatgga cgatcaatcc aaagttaaat agatctttag ggcgtaagcc ctattttttt
    10381 aaaattaagt gataacaaat aaatgatttc tgaaacaaac gttcttactc aactactcga
    10441 aacacacaag ctaattaact agattataaa ggagaatgcg taatgaaaga aatcagtgca
    10501 aatgaagctc atattccgca aaagaatcag gttggtatat ataaggtgct tcatggtgaa
    10561 ggaatttata aaatacagtt acttaccggt tacaataatg gtgcaaatgg gaagtggtta
    10621 aaggcaacaa gtattcgtga acttagaagt gaagtagaaa aaattaggct tggtctcaca
    10681 agtgaagaaa tagctaaact gaagggaaat aattcttttg taattgaaaa agaaaaggaa
    10741 aaacatctac tttatcgtga gctgaaaatt gaagaatact atgaaattga tttacctgaa
    10801 aaattaagaa ttattgatca acgttcagaa gaaaaatgga aaactgaaat atatccaagc
    10861 ttaaatgact acaagacatc aggaaagccc atgtataact acacaggctt tttaacctac
    10921 acacaataca aatatgcctt tcgcaaatta aaaggcatta aacaagctga aattgccctg
    10981 tatgagatga aaaaagtgga tagattgaaa ttgattcaag atttagtgca aaatacatgg
    11041 aatcaagcga ttgtcgagct agaaaaagaa aatgggaaaa ttttaactaa taaatatctg
    11101 atcgtaaatg aaattgaaga attaattggt ttattttcat gtgaaaaagg tgaagtcttc
    11161 ttgaagtcat caccaaccta ttcaattttg gacagtccgt atcagtttat acaggcaagg
    11221 agatgagtta gatgtctagc ttgaaagatg actatatcca agcaaaacaa gagtatgaaa
    11281 aaatacagat gttgaaatct gagaaagtcg aattattttt aacacaaaaa ttagcagagg
    11341 atcatattgc taacttagat gctaaaactt tagaggaata tgatcggcag tttaatcaga
    11401 tacatcacaa tatcattttg cttgttgagc aggcaaagcg aaggtttgaa aaagcggaat
    11461 atgatctatt agatcaagga attgtatgct tggataaggg attgcgaaga tattatggtg
    11521 cagagttaaa aaagcaaaat gtagatagaa atgcactaaa acatgaaatc ttagaattga
    11581 taatgcaacg aatttgaagg gatagaaagc aaatgtcaca aaacattagt gctgtaaaac
    11641 aattaataga agaagattta gagaccacag ttcagagatt agcggaacga atccattttg
    11701 caaattatca aaccatctct aactggtcaa gcaggaacag aaatccggaa gaaattccaa
    11761 tgaaattttt ttatcgaatc ggaaaagaat atggtgttga tccgataaaa attttcgaga
    11821 agaccatgat ttattactat catggaacac ctaacgaagt gttagaacat cttttggaag
    11881 aggcaaaaga aaaatcatca atattagagc tatcgcaaaa tgacaaagtt attcaagtga
    11941 aatgcgttga tccaacagga aagattattg tcggttttga tattgaaaga aaatccaaaa
    12001 tagattttat tacacaatta aaaaagataa ataatttaga tattctaacg cttagaccag
    12061 atcatgaaca gttgtttatg agttttaaaa aacaattgaa agaaatacaa aaaagatata
    12121 acaaggtgtt tcctaatgcg tgaaaagctc aaagaaatca gagttttttg gataattaac
    12181 aaagaatgta tcatcttata tactcttgga tttcttagca tgatcttgtt ttggtggtta
    12241 tatttaaaac agtaattgga gagggtcaag aggattttta tcaagataag gatgtggaaa
    12301 ctgcatgtgg aataatagat taaaaacagg attgttatta atagttattt cctgcgctat
    12361 gatgattggt atgaggatac aaagggaaca atcctatttt gaagtttctg ctaacaatgt
    12421 aatagaaaga tgccattatg gtcaacattt ttggactggg gaagtcagag aaaatataga
    12481 tcgtgaatat gttcaaagaa ttgtttggga tgcatattct atcaaagatt atcctaaaag
    12541 tttaacgagt agattatttt acagtgaaaa agataatcaa aaactctcag atttgatgat
    12601 gaaaaaagta tgactattat tacgaaagag aaacagagtg ctatattcga ggggaaaaag
    12661 cctacaacaa aagtgactat tggggtgaca aggcgaagcg attaactgca aattttagag
    12721 tgtatgaatc agagtttata aacttgacct ttcttaatag tgtttttatt gattatgtaa
    12781 ttactacgaa acgcattgga agttattcaa gtacctacgc atccttagtt ccaatgttaa
    12841 actcgatgag ccgtttctta aaagaaagag aaaagaaaga gctacagtat atcaaagaaa
    12901 tcatgccgga ttttgaaatg cacgaatggc aaattgcttt atcagattgg aaattggcga
    12961 ataaggttcg tagaataaca ccttttcaag ccaagcgctt tttgagagct atgaaatttg
    13021 tgcaataaaa aaagccacca gacaagttga tggagactaa cacaattacc aacagttgta
    13081 attgtgttag cttacaaatt aattatggtg tttagtcaat cccaaattgt gaaatggcac
    13141 caataattgg aaaggaaaaa tgaaatgtct gaagatttac gagagctgaa gttacgaaag
    13201 aagcagtatg atcagtattt ttccggtttg ttgaataaac atattgaaga aggacttgat
    13261 catgattttg gaaaagatga atattgttta tattgcgaac gttacaatga aaaaatgtct
    13321 gaatatctac agctgaaaaa agagatacag aaattagaga aaaaagtaca tgcttggaag
    13381 tataaagatt ccatgtctgg ttatgttgaa tctcaaaata agccaagaaa aaagaagcaa
    13441 gcgtatgttc taaagatggt aaatggggaa gagttaattg tattagcgta cagtgaagaa
    13501 gaggttcgag aactcgttga aacacctgtc aggaaaaagg ttgaaacaat aaatgagata
    13561 gacgacagcc aatacaatag catctatatt gaaacaagtc atggtggata taagaaatta
    13621 gcaactttcc ttagaagttc taaagttggg ataatttcta aaaaagacat aagtgctgtt
    13681 accgatagtt tttgtacttt atctgttcct aaagcttatt tccaatacga tagtaaaaga
    13741 tttgaacgct tagagaaatt ctgtagaccg tatactgtat tgaaaactaa aaaatttaat
    13801 aaaactaaaa gagtttctat acagttaccg actggtcaaa tctcattagg tgaaaaagac
    13861 tatctgataa aagtaggaaa agagttaatt ataaaagtac cggaacactt atttaaagaa
    13921 aattttgaga tacgtgaaac cttggtatgg aaagaagaaa aatagtttgg aagacataga
    13981 atggtataag gggaaattga aatggcagta aataaaatat tgcaggtact aatcatagac
    14041 gaatttggcg gaggagaatg gtttgatgtg cagtccatag actttgaaga agaagtcgta
    14101 gttatagaaa atatttacgg cgaagtatat tcatattcgc ttgaaggtga gaatattgag
    14161 gatttacggt tgattggtga gtagaaaaaa acagaaatat caaaatggag ataaaaatta
    14221 atagcatgta aagatagtca tgcttgggtt aaaaatgtta aaattcaaaa aaatgagtca
    14281 gaagggataa aagtatgaaa agagaggaat acaaacaaag attaaacgaa ctgcttgaag
    14341 aggacgaaac attaactcat ggttcgcctg atgaaattct atacatgatt gataatatgg
    14401 tgatctttgg tggatatgaa cttggtaata ggtcagttga tcataatatt ttagaatttg
    14461 atgatgtatc atgggaagaa attcttgatt ggggaatttt ggcggtacct gaaactaaaa
    14521 cctatattag tgacactatg gttccttttt ttgaggaatt ggattataaa agacttccca
    14581 aaaatgaaaa tcatatactg ggaggaaact aacaatgtta agtgacttat ttattttact
    14641 gatctatcta ggtgtaagtg aaattattgc gtattttttg tttgtcattg ctaattatgg
    14701 tatttccagt gcaatcgttc aaatgtttag attaccagta agtgaactaa aaaaatgatg
    14761 agattatcag catatctttt aatgtttttg aatgtggtca cgttaatctt tgtagtaatc
    14821 ggtgtgactt tcgatttaat ggaatagtgg gaaatctata atggaaaaaa gagacacgaa
    14881 tgaacatatt ttttgaatca atattgttga tggtttttgc gatcagcgct taccagaaat
    14941 ctaaaaaaga gtaatgattg tttaatcatc actctttttt tgtagggaaa ttgaaatatg
    15001 tggagcttgt attggtttag ttaggattgc ttggctataa ggagctagtt ctttatcaaa
    15061 accatcaaag taggttttca gtagcgattt aactgatcgc tcatcttgtt tgatttggat
    15121 aatttcagca tccaaatcgg ttgtgagatc aggagctttt gatgcgatta cctgattttt
    15181 tttatcttcc aactctctta ggctgtttaa caagttgtaa aaagaatcat tttgattgtt
    15241 cattccaacg tgagttgcaa ttttaacaga agtttcgtcc gtattgtcat caaaagaact
    15301 cattggaata tcaagtagta acgaggatgt ttcttttact gactttttct ttacagcatt
    15361 ttttgttttt ccatcttttt tgctctttac tcgagatgtt tttggagcta gtttaggtaa
    15421 atcacttgtt ttatcagtca tttattagct tttcctttct attaatttat gttattatat
    15481 tatcataata acataaaaaa gtacactttt tgggggcgta gttattgaaa aaatctaaga
    15541 gaaagattgc cacattgctt atgttaatgg tgcaatttac agggattgca atgagtgtaa
    15601 tcacaataaa tccgcaagtg atacatgctg atgggatagc tacttatgat gcacaattta
    15661 actgtactac tgaaggtagt gggtcaggaa cagatagcga tggaaacgat actggaagtg
    15721 gcgctgtaac aggtgatgta gccaaaaata ttgaagatat ttacaaatac atgaatggag
    15781 aatatggctt atcaggtgct agtattgcag gtattttagg taactggaaa caagaatcag
    15841 gtattgatcc tcgagcagta gaagggtatt acggagatgg acagagagaa gttgacttca
    15901 tcaaacaatt tactgatggt ctgatgggta ttggctatgg tcaatggaca gctgggcgac
    15961 atacagcatt agttgattgg tctaagaaaa ataatggcgg ttcttggtgg actacagaag
    16021 cccaattgga ctttatgttc aaaggtgatg ggggattcgt aggaatatta aaagattatg
    16081 ctttaaatgc ttctgatgat gtcgtagaaa atgcagtaac ttttcataaa aactgggaaa
    16141 tttcagctga ttccgaatca gctgtaatag ctgtacgtgg tgaaaatgca aaagaaatct
    16201 ggaagtacat gaaaagcaag ggtatggacg gtgcaaaaga tgaaagtaag atcaataaaa
    16261 tatccggagg ttcaagtacc ggagatggat cgaataattc atcaagttca gcatcaggaa
    16321 cagtagaaga tgcctgtgca actacggatg acgctaattc tggttctgga tcaattggga
    16381 aagaaggaaa aattaacggt aaaaaaggtc aagtaatagc gaatatgact tatgaagaag
    16441 ctttgaaaaa atatgggaaa gcgatcacgc taccaaaatt caaagaacca gattggtcaa
    16501 cttcaccttt taacggaata tatcaaggtc agtgtacaga gttgacgtgg gcttatatga
    16561 gccaattata cgatgggaat caaccgacta ctggaaatgg tggttatgta tgggaaagct
    16621 acaaatcggc aggagcaaaa ataacgaaca aaccaatcgt aggttatggg tttagtgctt
    16681 cggatggatg gttatgggct gttggagatg cagggcatac tggtgtagtt atagcggtgt
    16741 ttgaagatgg atcatttcta tgtgctaact ttaatgttcc accaacccaa gctccaacaa
    16801 ggggagtttc ggtaacgttg attgatggag tggacggatc agataatgtg catttcttta
    16861 gcggtgtagg aaaagctaaa tttgaaatcg aaaaataaat tggaatgaag gacgatatag
    16921 atgaatagta aagtgaaaga tacaattatc atttctttgc tttctggtat gttattagca
    16981 ctgcttgttg gaatcctatt cttcaataag cagaatcagg acatagaagc tgaaaaacac
    17041 agtgtgcata ttgaagataa gctgacattc tttaaagagg ttaaaccaaa tgaggtggat
    17101 ggattacgga aagatttaga aaatgatatt caagaaatgt tgaatggatc agttcaaaat
    17161 aagtttgcag atcaatcagg tgaagaattt ttgagaaaac tatttattgt aacaggtaaa
    17221 ccaccaatta ctgataagag tagtgagaaa gaaaaaatcg agtactataa gccttttact
    17281 tttaagctaa caaactttgg aattaagaca tctagtagtg ggttgtatga agcattgacc
    17341 gattttgaag ttcaatacaa tggaaaaaaa gtgtatgata ccacattaac catcacacta
    17401 aataatgata agaaagtgac tgggggtgct ttttatgcaa aatcatgaaa tcactactgc
    17461 taaaaaggcg atcggaatac tttccataat tgtgatcctt tgttgtgtgg tactatttca
    17521 aaagaaaaat gcttatgata ataatgtcaa agaattggaa gaccttaaaa gtaaacaaat
    17581 cgaagtagtc aagaataacc agaaagtgaa agctatgcag gaagctcaat acaaagaatt
    17641 gggaaatgct gaaattaagg aacaaagtag gaaattcttt aatttatttt acaactggaa
    17701 ttcatgggaa aaatatacga agaatatgaa ggaaatacaa aaagaatttc ctcagatagt
    17761 aaagtctagt cctgttgata tttctggcac gatggtaggc actggtaata gtccaatatc
    17821 tagctatact gctgattact atacaacaaa tcaaaaaggt aaaatggttg aattgattac
    17881 acagacaaga aatactccaa caacagcaac aacaacaatt tggcgtggag taacaagcat
    17941 aggagaaaat caattgtttt atttggagga tttagatagc tatgtaagag cagagtaaaa
    18001 taaaaaaaga gctaacggta ccaacgttag ctctttgtgc tgacgatgat aacaaaacct
    18061 accgaaaaag gattgtttca tcacaaacac aaacagaaaa gaattgataa aaaacccgaa
    18121 cctcagaatt tttattaatc aattcattta gattataata aattgagctg aacattacaa
    18181 tacgtatatg ttgtaaaacg taaaaaaagt caaaagtaaa tactttctta aaaagtcaaa
    18241 taaaactatt aaagacaata tatatggttt atttgacata agaaaatggt ctagatgata
    18301 attgtaatat agaattgtag acttaaataa agcataaaat accttttatg ataaggtata
    18361 gctcttaatt ttaagcagtt ttagatataa gattgaaagt aaagtggggg agaataataa
    18421 tggcagatca taaattagaa aagttattag aagaaacgtg gaatccaaaa gaatttagtg
    18481 aattttttat ggagaatttt gaaactgact tagcagtgat tgttaaagat gctcttagag
    18541 agcaagggta tcctgaaact gcaaactata taaacattaa ttttactttg tatactgaaa
    18601 ataaaggaac ttgggatttt tgggctactt tagcaaataa ggagttaagt gataaatcag
    18661 acacaggaat tcgtaacttt tttgaatcaa acagagatga ttatatgtat gctaacaatc
    18721 aaaataaatt gaactttaga gtagagtttg atgaaacacc tgaagaattt atagaaagac
    18781 aaccgcctaa agaaaatgta gcaaaggtat tggaagatcg ttggaattca gatgaaatag
    18841 tttcaacaat tagcgaacta ggcggacaat atgaaccgct agtagaagcg gttagagaag
    18901 agctaagact gaataagttt cctgatgttc aaaatattga tgtttctcaa attgaaataa
    18961 acgtcaagat cacgaataaa cttgattatg gtagttgggc agatatagca ttagaaaaat
    19021 acatctactc aacgttaaaa gagtttatcg aaaatagaat ggatatcatg tatctacagc
    19081 atccacagta tttaaacttt ggtgttgaaa tagctacacc tcttgaagaa tggaaaatgg
    19141 agcaagggct tgactagcct ttgctccatt tttttatttt acaaatgaat acagttcgtc
    19201 gtttttacca agaatgattg tttgcccaac tttgagattg tcaggtgatc cttcagcggt
    19261 ttgtaaggag aacgttccat tgaacttatc ggataccgtt ataggtagct ttaattctga
    19321 tccttcctca tctttgagaa ttgctgtttt tcgttcatca aaataaacag tctttattaa
    19381 ataatttttc gtagtgatct cagcagattt gtattttttt gtagtagtac cagtttcttt
    19441 agaattagag gtattttcta cgactattcc atctttggca tttgagcttg tgtttatttg
    19501 attgaatatt ttcagcgaca agataacgct aagaatagtc aatatagaaa caagtgtaat
    19561 taatactgat aatttccttt ttttgttcat tttttcttaa catccttttt tgttttgatt
    19621 agttagatat tccttatcta acaagccttt ttaggctatt aaatgaagat aaaccccaaa
    19681 aaatattgca attatgacag tgcaatggta ctattattat acaataaatt ttgcgaaagg
    19741 ggtaggtcta aatcgcaaac aaaaagaaat tgaacctaga caaatacaac ttgtataccg
    19801 caagggaagc aagtttgatt ttgggtaaag gtagaaatta tgttacaaac ataatcaact
    19861 attatccaaa tcgtttgcct aaaggttcgt tcaagaaagt gggtgacaaa acctatatta
    19921 taacggaaga aggaatagaa gcattgcgat cacaaacaga aaataaaaac aaaaagaagg
    19981 aatgaatatg aagagaaatt cgcttggtat ggttttattg gcatcagctg tacttgcggg
    20041 gttatcagta ccagtaacct cacatgctga tgaatggact gcaaacacac cggatcaaat
    20101 ccaaattaca acaggacaat caagttatgc gttcaaaaaa ggagatacta tttggtcaac
    20161 tgtagagaag cttaaaagct taggttacaa gactactgtt gctgagtttg ccgaattgaa
    20221 caatgttgat ttggcaaaag gagaacaacg gactatcccg attggacgaa ctttcaacat
    20281 cagtaaagat acattaacag ttaaggaagc agatggcgaa gtagttagtc aaactgttca
    20341 aaaagataaa cctgatccta ttgaaaatac ggagcaagag aacaaaaaat ccgagactac
    20401 aaaagaatcg gaaccaacag caaacacttc tgataagaca gaaacaagtg cttcaagtgt
    20461 tgatggagca agttcatcta atacaaataa ttctacaagt gagaaagaag catcacaggg
    20521 aacaagtgaa agtagcacta cggaagctga tactggaaca tcaacaaata actcacaatc
    20581 aacatcaaat gatgcaaatg aatcaaccag tacatcttct agtgatcaat cacaaacagg
    20641 ttcgagtgat gaaactgaca gttctacttc accaagtaaa cccgaacaac caactaatgt
    20701 tgtgctaggc gttccattta ttagtcaagg taacacaatg ctttgcgaag gaacatcttt
    20761 gctagaagcg ttacattata aaggtgttgc aacaaatcaa gatttaatga cttttgttaa
    20821 atcaatgcca ctatctcctg acaataatcc aaataatggt ttttctggtg aatggagaca
    20881 caatgtggat ggaacttatc aaggtatgaa tccggctcct gtagtttcat ggggtgttgc
    20941 taatggcgga aatgttgtag atatttctgg ttcatcggta caaggattaa aagatgagat
    21001 tgtaaatgga aatcctgtag tagcgtgggt aacttatgcc tttgaacctg ctcaacataa
    21061 accaatgttt tggggcgatg ctatttggaa tcgacatgta gtagccgttg atggatttaa
    21121 agacggtttt taccatgttg tcgatcctgt gtttggggca agttggatta atgcaggatc
    21181 atttgaaaca gcatataacg tatctcaatt agcggtttcg gtacgttaat aaaagatatc
    21241 gtttatatac taagtagtta tatagtcttt atttgtcctt gcttaactac ttagaaaact
    21301 tgttgctcat ggtgggtaga actaaaaggg gtgatgctat gaatactaga gcgttgaaac
    21361 agcttgcttt caacgaaaaa aatgattaat aggtgtggtg attttaactt aggaatgggc
    21421 gcgaaggtca ttaccacacc gacatatcgt ggctcatttt cttcgcctaa ctggtataaa
    21481 gaaggagtaa cgagagatga agcatgaaaa aacgataatt ttaaaatcaa aaataattta
    21541 ttgttttgcg actttagtaa tgatgttatc tacgcttata atgccttttt cagcaataag
    21601 agctgaagca gatgtcacaa aaccgacaag tgcaaaattt atagaaactg gcgtaaaaac
    21661 agacggttat attagggtta atatgccgaa tcatccaaat gaatggatga tttcaagtca
    21721 atttaaagat tctcatggaa atattggata ttgtatggat tcagaattgc catccccaac
    21781 aggaagtgga gccggaagct tgaagtacaa gggagctggc agtgatgagt tctatcgtat
    21841 gtttaaaggt ggttttccat caaaaacagc aaaggaactg ggtgcaggaa atgatacaga
    21901 agcatggtat gcaacacaat tagtttcatg ggttttagcc ggaaacttta aagtttcaca
    21961 gattgtatgg tcacatccaa accataccgc cgcagaaaca gctcgtgtaa aaaaagcatt
    22021 tgaaaaaatt tatgactatg ctaagaatgg aaaagatacg cccaacactg agttttctat
    22081 cacagcttct aagacagctg acgaaggaaa atatcacact ttcacatata aaacagcatc
    22141 aaataagact ggtaatgcga agctcacttt tacatcagca aaaccagctg gtatgaagat
    22201 ttatgatgca gatggtaaag aaattactaa caacactgtg aaattaaatt caagttttac
    22261 tattaaagta cctgtgacta caccaagtgg tactttgtca tttaaaggta ctgctaatgt
    22321 tagcacaacc aatcctttta cttttgatgg acgaggtgtg tatcaagatg ccgtagttat
    22381 gatcactact tcagaaacaa aggactctaa atctttgagt gctaaatgga caagagctgt
    22441 tggtcaggta acagttaaga aagttgatca agatggaaat gctttaaaag gagcggagtt
    22501 tactctaact gacaataaca atgctaaatc tgttaagaca accggagatg atgggttagc
    22561 tacgtttgat ttgcaacaag gcttaacata cagcttagaa gaaacaaaag ttcctgaagg
    22621 ttatcaaggt tcatttaaaa aggatggtat tactgttaat accgatggac aagaatttag
    22681 ttacactgct acgaacacaa ttaaaaaagg taaagtaaaa gttaaaaagg ttgaccaaga
    22741 tggaaaacca ttagctaatg ctgaatttac cttgacggat gattcatccc aaacacaagc
    22801 agaaaaaaca gatgaaaacg gacttgcaag cttcgatatt gtagcaaaca agacctacag
    22861 ccttgaagag accggagtac caactggtta tacaggcgta ttcaagcaag aaggaattac
    22921 cgttaaagat gatggacaaa cttttgaata tacagctaca aacactttga aaaaaggttt
    22981 agtaaaagtt cataaagtcg atcaagatgg aaaacctttg gcaaatgcag agttcacttt
    23041 aaccgatgaa aaaggtgcaa cacaagtaga aaaaacagat gaaaatggac ttgcaagctt
    23101 caatattgta gcaaacaaga cgtatagcct agaagaaacc ggagtaccag taggttacac
    23161 aggtgcattt aagcaagagg gtatcaccgt taaagacgat gggcaaactt ttgaatatac
    23221 agctacaaat aatttgaaaa aaggtgtagt aaaagttcat aaagtcgatc aagatggaaa
    23281 acctttagca gatgcagaat tcaccttaac ggatgaaaaa ggtgtaaaag aagttaaaaa
    23341 gacggatgca actggtctgg ctacgttcaa cattattgcg aacaaaactt ataacttaga
    23401 agaaacaaaa gtaccaacag gttacacagg cgcattcaaa caagaaggaa ttaccgtcaa
    23461 agatgatgga caagtctttg aatatactgc taaaaacact ttgaaaaaag gtgtgatcaa
    23521 agttcataaa gtggatgatc aagggaaagc attagcaaac gcagaattca ctttgacaga
    23581 cgacaaaggt gtaaaagttg tacaaaaaac ggacagcaat ggcttggcaa catttaatat
    23641 tgttgccaac aaaacgtaca aaatcgaaga aacacaaaac cctaatggtt atacagggaa
    23701 atggtctaaa gaaggcatca ccgttgctga cgatggacaa acctttgaat atacagtaat
    23761 taatcacctt gtacctgaaa cagtaagaac aggcggaatc ggtacatcag ccttccttct
    23821 tgtatttcta attgcaggat tgagtatggt gctactgtat tatcgagaac agcacaaagc
    23881 aaaataaaaa agtgttaatg gaaaagaagt aaatatgaaa aaagaaggct gacgtatgaa
    23941 atcaaaaaaa attttttcaa atttaatgct atttggtact ttgctaggag tgggattggg
    24001 cgcaagctca atctcttcta gccttggact aggggggcaa gtagcatatg cacaacaaaa
    24061 ctataattat caaggaactg gaacgattca tatccataaa gtaacaaata ctaatgggga
    24121 tgggaaccct gtctcagata ttgataacaa agggaaagaa atagctttac caaatggtgt
    24181 aaaacctctt gaaggcgttg agttctctta tgtgaaactt actaaagatg aagctaaatc
    24241 cttgggatgg acggatagtg atactacaaa aggtcaacat attgatgatg ctaaagcacg
    24301 attattacta aacaaaaaat acagtgaagt aagtagaatg aagactgatt cacaagggaa
    24361 tatagacata cctatacaag taaatacaga cgatttaaca gataacttgt atttgattgt
    24421 tgaaacagga acagtcaaag gtgtagttga agaagcgtat cctatgattg ttaacgtacc
    24481 tgaactaaac ggatcagatg atcaatactt taattatgat gtacatgtct atcctaaaaa
    24541 ctatttccaa ttaaactcag cagagcttga taaaaaagac gaagatggaa aggctcttgc
    24601 cggaactgtt tttgaattgc gaaaagatga tggatctgat caggttgttg gaacttataa
    24661 aacagacagc acaggtaaat taactgtaga tggtttggaa ttaggtgact attatttcct
    24721 agaaaccgat ttgggaacag atgctaataa aacaacctat ttactagatg gtaaaaaatt
    24781 caaatttagc gttaaacgtg gggagactgc taaagtagtt cttgatgtta ccaactacaa
    24841 acgacctgta attactaagg taattgataa agaaaccgtt gatactggcg aagatgttca
    24901 atatacattg aaaccatcaa ttcctggtaa tattggtaat tacgaaaaat atgttgtcaa
    24961 agatgttcta ggtgcagagt taacttatga ttcatcagaa gtgatcgaag gaatgacttt
    25021 agatactgat tacagtatcg aacgtgacgg acaaacgaca aactatgtct ttactgaatc
    25081 aggtatggat aagcttcaga aacaatttga tgcaggggat cgcttcttta caattttgat
    25141 gaacgctcat actaatagta aagctaaaat ggatacacca cttaagaatg atgtttcaac
    25201 gatctacaac aatggatatg aacccgaaga cgaaacacca gttgatcacg cttatacaat
    25261 cattggtgat caccaattca ttaaaacaga tggagataca agcaaagcat tgagtggagc
    25321 aaaatttgta gtgaagaaca aaattgatcc gaataaagtt acttacctaa aacaagatcc
    25381 aacaacgaaa tctgttactt ggattgcaga taaatctaaa gctactgaat tcgtttccgg
    25441 atcagatggt aagttccaaa ttgaaggctt gcaatatacc tatgatgtag attttgataa
    25501 acaaggtaat ccaacaatca cagataaaaa aatcaatgat tatgctcttg aagaagttgt
    25561 tgctccttca ggatatacac caactagaaa tgatgttcca tttgtggtat cgaatgatga
    25621 tgcttctagc gaagtaacag acatcaagaa ccttaaaaca cctgaaacag ttcgtacagg
    25681 tggaattgga acagtagtat ttctagtggt tgcaggtgtg gcaggaacag ggctatatgt
    25741 tgtctacaaa cgagaacaac acaaagctta atgaaataaa attccctaga gtgtttttgg
    25801 ctctagggtt tctttttaaa aagtgtgtga aaagtaggta tgagcatgaa aaagaagaat
    25861 atcaagttga tcatcttaaa aattgggatg ttgctatgtt taggagtagg tctatatcct
    25921 gtaattactc aggcttatac ctactaccaa actcaacaaa cactaaactc ctataaggaa
    25981 aaatatgatg aagcttctga aaaggagaat gaagaagtct tcaagagata ttcagaagtt
    26041 aataggaaac gaaaagacac agaacaaacc tcagtcacga ttcctgaaga taataacaat
    26101 attccgtcaa atggaaatct tcagaaggat aaattaggtg aattagtggg tgaaattctt
    26161 ataccaaaga ttaatgtgga tttgccaatt tatgatggtg taaacgattt ccaactatca
    26221 gcaggagcag gaatattaac cggaactgat tatccaactg gtgggattgg aaaccattct
    26281 gttatatcag ctcattcagg cattcctaat aaaaaatatt ttacagattt agagttgctc
    26341 aaacttggtg ataaattttt tattgatatt tcagggaaaa aacttgctta tcaagtagat
    26401 cggataaaaa caattctacc aaccgaattt gaggatttgc ttcctgtggc aggaaaagat
    26461 tatgtaacac ttatgacatg tacacctgtt ggagataatt ctcataggtt actcgttaga
    26521 ggggttagca taccttacca aaaaagtatt gttgagaaag aaaagaagaa agaagattta
    26581 aaggtaaagg cgcagatgaa ctggtatcct atcgtagttg taggggtttt attaataata
    26641 gctgtgatag tcattattaa aaaaatagta gcaaagagac gaggaatgaa aaatgaagaa
    26701 taaaatacta ctttttttga ctcttatttg cactttggtt ttaacaattt atcctgcaaa
    26761 tcagctctat catcaattca aaacaaaaga gaatgtggcg gaaatccaag agaaaattga
    26821 gaaaaaacaa actccttcaa aagaggaagt ggcaaaaaaa gtcgaacaaa taaaatcgaa
    26881 cagcggtaac caaggattag acaatactca gaaaacagat aaaggagtag acaatactca
    26941 ggaaacagac ggtggatcat ttgatattcg tgaagctgaa ggaaaggtca atccatacac
    27001 aggagatgtt ttttcaaaaa atcaaacgaa agattttgaa ccatctacct attcaggagc
    27061 ggttggttat gtctatattc caaaaataaa tgtgctagag cttttgtttc taggcgcttc
    27121 acaggagcat ttatatagag gtgtggcaca aatgagtgct actagtctac ctttatcagg
    27181 taaaggtcat caaacagtaa tagcaggtca tcgtggcggt tatacgtcac cacaatttct
    27241 ttatgttgat caattagtag ccggagacca catttatata gattatcttg gtgaaaaagc
    27301 agtttatacg gttttccgaa cagacgttat tagaaattac ggtcagaatt caacactgac
    27361 tgatattcct gatttagata tgttaacact ttatacttgt catccatatc ctcagaacta
    27421 cgatcgttta ttagttagaa gtctaagaga tgaaaaagca aaatacatca cgccacaagt
    27481 aacagttggc aataccgcat acgctgatgc cattgatagc gagtccgttt cagataagac
    27541 agaaactgtt acatcttcta atgatttatt tgaagatgtt gatcgtgcca acaaggaatt
    27601 agatggaaat aatgatctta atattccgga acaaggtagc gtagagatgc aagacagaac
    27661 gtttgctgaa aaggcaagtt caatatttac aggaattacc aataaacatc agaaaatgct
    27721 tttagtcatt tggactgtaa taggagcatt agcaagtcta aaggttctta ttcttttaat
    27781 caaagagttt cgaaaagcat aatgaagatt ataaaaattg atagagtaca gtaaaaaggg
    27841 attgatacta ttattgatct cttttttatt ttgaatagag ctagattcac ttttaaataa
    27901 aatcaaaact gatcttaatt ctaaagaaaa agtcaagaaa aaaatacaat aaattttttt
    27961 atcagaatat ttatcagaat attattagaa aagataaact caaaagctta atttaatagg
    28021 gttattggtt gtatttagaa ataatgtatg gaaaagctat aaagcaatag ataagatgta
    28081 gaaaatctta tttgtgaatg aaaaacaagt catcgtaaaa tattaatagg aggtgtagta
    28141 atggtgggag tatcaaaaac agctttagac tcattagcta aactatttag tagcaggttt
    28201 gatggtgcag aatttgttga atatctaagc caaaacgaaa gtggaaaaac tctgatcgaa
    28261 acaatgaaaa aagactattt gaatgaaaat gactttccga catggacgaa tattgaagat
    28321 gctacatggg atgtagtatg tacgatatat tctccttggg taacgattgc tgattttgtt
    28381 ttagataaaa ttggagggga tacataccct gatgccaaat ctcttgctac aagtgacttt
    28441 aaaacgttct atggttatct aataggtcta ataggcaatg gaatagattt gaaagttacg
    28501 ccaaaacaaa cacctgaaga aattgaaaaa gaaaaaggag aggttcaaat ggcttatgca
    28561 aatgacagag aaaaagtaaa attattggaa ggtaaattat ctgaagaaga acttggaaaa
    28621 atggtgcaag aaatcaatga agacagcaac tattttcctg aattgaaggt atatcaaaat
    28681 gacgaagatt tctttaatga aatgtttagt aatagtccat gggaagcagt aagtaaaaca
    28741 aatttaggag aatggaactt tcaagatgat ctagttgcat ttagcaatga tgggtattta
    28801 cgatcatatt cagaaagtga gtatagagct gaattaaaaa cgtatgaaac ggatattctg
    28861 atgagctatt taaatctatt agagagtggt cagggagagt taagagataa agttgaagaa
    28921 ctattaaaag aagagctata gggattattt caacataaac acaagcagaa aactaccaaa
    28981 caaaacgtac catgcaaaaa gtcggttaaa ccggcttttt gctctattat gagaggagat
    29041 caaaccatga gtgcaacaac cgatatggct ttagaagcag taataaaaga taatacagga
    29101 aatgtcgaaa atgtagaaga ctttagcgat attgcaatca atctttcagc atggatttca
    29161 gctgtaggag aagaaaatat cgaatttgag atcaagaata tggatgactt aaaagcaaaa
    29221 tttcctttta ttgcatcgaa actaagtcaa gttgccgagg ataaatcgtg gaatgaatta
    29281 gttagttttt ccgaaaaaat ttatgatatt acgcaagaag atgagtgtgt ttcatgggaa
    29341 atgaataatc aatacaagaa aattgacgct ttcgtcaaat cacgtataga aggaacagct
    29401 tttgagtttg aaggtataga taccgcagat aattggttaa ataaagaatc ctttattaaa
    29461 agtatagcgg atgatcttga aaaacaatgg tctaaggaag actatgcaga gtacctaaaa
    29521 acagaaagag atacagaagt gaaagaaaaa ctcactgagg tatgtttgga tgaacatatt
    29581 gatcccaata tcgtaaaaca agaagactat gaagtggtta ttgataaagt tcctactgat
    29641 tttgaggaag ttgtaaagta tatagttgaa gaagcattat cgcaatactc ttcagatgag
    29701 gaagtggacg aagaaactgt tcgtaccaga gtttggcaac tggtttttga tgacaaagat
    29761 tataaatatt tgagtgatca aaactcggtt aattggagtg tacgattatt aattgatcaa
    29821 aatgatattg aatagagctg aaaagctcta ttcagattgt agacaaaaag gagttggtaa
    29881 ttaagccact cctttttgtt agtctttgca taattttgaa ataaaaagcc ataggctttt
    29941 ttgttttcca tagccatgtg gctagttttt ttaaattcat ggcagcaaac gtaagcatcg
    30001 tgtgcgtggc gactttttca agacctcgat atttcgtcca acgcatacca tgcttttctt
    30061 ttgcatctgc aaatattcgt tcaatggttt gttttctctt tttgtaaatt tctctattca
    30121 acgccgtatg gcgttggtgt tcaagttcat ccataagatc agcccaaata tgtcgagtca
    30181 ctattttttg atgttttgta ctcaaagtac aattttcaag taatggacaa ctcacacaga
    30241 attttggatt tgatttatag gttcgataac catcacgagt gatagttgaa aataacaacg
    30301 gttgattatt gggacataaa tagcagtcaa aataggcatc ataaacaaag tcttttttac
    30361 gaaacattcc tttcactcca tgaggtgcag tatatggaag aatcgggtag agtttcaagt
    30421 gtgacagaaa atgaacaaat ttaggcgtct tatatcctgc atcagcaacc acacgcttta
    30481 tatttggaaa agctcttttc gactgtttaa ccagatcgat tgcgacttgg ctgtcatgca
    30541 catttcctgg tgtgattaat gtagataaga taaaaccgtt atcttcacag gaagtatgtg
    30601 cagaataagc aaattgtttt tctcgttcac ctttaacata gtaaccactt tctggatctg
    30661 aatgacttat tttttgaaga ctcaactcac tttctgtgtc ccatgtaaaa ggacgttttc
    30721 ctacagcaat acgttcttta tttatttcat cttctaattc atcttgataa gcttttgctt
    30781 gaccgtaggt catctctttc gtgaacttat gtttattggc atttgctttg atatgagtag
    30841 aatcaatata aaggttgtct tccgtaacga agccagcttt tacagcttgt tctagaatat
    30901 gagagaaaat atcttcaaat aaagttgttt ctctaaatct tcgaacatag tttttaccaa
    30961 aagtagagaa gtgtgggata ggatcctcga atgaataacc taaaaaccaa cggtaagcaa
    31021 catttgtttc aacgtcttta attgtctgtc tcatcgaacg gataccaaat aaatattgga
    31081 taaatactaa tttaattaag accactggat caatactggg acgaccatag gttgaatacg
    31141 tagattctac aatgggataa atgaaatcaa actgaatcgc agcatcaatt tttcgaacta
    31201 agtgatcttt aggtacaaga tcatcaattg ttaacatagc aaattgattt ctaccttcaa
    31261 tcgattgttt tctcatcatt agaatcactc ccagaatagt ttatacaatt agtataaaac
    31321 aaaaaactgc aaacaacact ccaaatttag gagtttgtct acagtctgaa tagagctgaa
    31381 aagctctatt ttttttgata aaaaaactag ctattttatt aaaaataatt tggttcaaaa
    31441 tttatttttt taacttgata attttttgcc atcctttttt aaattggcta tatatcaagg
    31501 tttatagtgg gaataaataa aaatacattt gacaaaattt acaatatagt ttattatata
    31561 tttatcttat tatgataaca caataaaagg aatggtggta tgaaaaagaa gaagtttagc
    31621 cgatcgttgt taggaatttt ggttttagtt gcggttggtc ttagtggcaa gggttacttt
    31681 cagaataaca gtactcagga acatgatttt cagatcaaaa gtagtgttca ggtaggtaat
    31741 aaagcagaca gtgaatcaaa agaattttta gggatacgtt atgatccaaa caaatatcca
    31801 aataatatcg taaacattaa tgatgggaaa tatacactga caaaagaaga ccatgaagca
    31861 ttatcagaaa aaggcagtca aaccttttgg gtaaattatg cagatttaga tcaacttgga
    31921 agagcaggag atgttgaagc gttgatcacc tatgatgctg taaattctca ctcatctaaa
    31981 aatgtggaac gtccatcttt caaaagttct gtccatattg caggagaata caaggatgga
    32041 gtatttgatc aagagagaga tacttgggtc ggcaaagatt caaacaatga aatcactcag
    32101 ctagaaggct ataaaggtta tctctataac aaaagtcatt cattagcatg gtcgctagga
    32161 ggagatatgg aggtacacaa tttaacactt ggaacgagag cgcaaaatgt tgggaaaaat
    32221 gatcagaatg gagggatggc ttactcagaa acgattgtac gtgatgcaat ctataaaaat
    32281 aagaatgaaa aagtattgta taaagttgat ccaatttata aagggaatga attagttcct
    32341 cgtggttctc atgtcgaagc tttctcagtt aacgataatg gaaaaacggt aaaccttaat
    32401 gtttatgtat ttaacaatca aaaaggaatc aacatcaact atcaaaatgg tacttgggta
    32461 agagattaga aattgagaag gtgaagaatt ggaaacaata agcatcaatg gattagataa
    32521 atcagttgca aatcgaataa aaaaaggaaa tggctactat cttctgaaga agggtaaata
    32581 ttcagttgct agagagttag aaacaatgag tatcgtgtat gtagctaaga atcattttga
    32641 cgtagaacag gctatttttg atccagattc tgaaataggg aaatgtgttg aattatcaga
    32701 gaaaactgtt agtacaaaag gggcgatgat cattgagaga gaaatgttag acgaaatcag
    32761 agtaatcatt cctaaagata cgcagatgtg tgaggacagc ataagtgacg atgaaccaaa
    32821 atcagaaaga aaataaggag atattcagat gagcgaaaaa tccattgaat taaaggaata
    32881 taagcgaagc gatggagaaa aatattatcc tgtagccagt ttttctaaat cagtatatct
    32941 ggttggacgt gcattggtag ttggtgcaat tggcatatat attttgcatt gggtaacaaa
    33001 ggttgtacaa ctgcttgtag ataccttagt aagtattctt gtctcgttaa atgatgttaa
    33061 cggaacagat gatttaggat taaaagagat gtttaaagag ttactcaatg cgttttttcc
    33121 caattattta agttattcat ataatgtaca aagctttttt gcaaagctac acttggtaga
    33181 catctctatt ttagtattag aagcaggaac ggtattcttt gttggatatt tggttacttt
    33241 gatgttcagg catcactatg gagaacaaag accatttttt gatgatatgg aaagtagaag
    33301 gttgaaaaga gaagctctag aagctatgga tgtgaagtat gatccaaaca agattatagg
    33361 agatgaaacg aacaacaaaa aaagaaaacg aaacaaaacg caattagaat ccgatgtgct
    33421 atcaacgctt aaaaatatca aaatttctgt tcatacaaga cgtgatctaa gtgtcaatga
    33481 agatattcga gaatatatcg taaaaatccc gcaaccaaaa gggaagaaag ttagagaaac
    33541 agttttacga caaattcaag atttagggtc gatcctgaca agagcaacaa gcggacgagc
    33601 caaatttggt gaaaaagaag atgaagtttc aggcgattat ttcattttcc gagcatcagt
    33661 aaatattgaa agagaacgta aggaaagagt aagtaccaaa aacaaagaaa cggaaagagc
    33721 aacatcttca atcgcagttt caaattcaca attttctttt ccaatcaatc tctttgccga
    33781 taattccaaa aatatccaaa accaaaccga aaaagctaga aaatatgccg aaactcaaaa
    33841 gcaatcttta gaactttatc tatcttctgc tgacgtacct gcaagtctaa cagatatgca
    33901 cgttgggaat acatctattg aatatttgtt tactttatct cgtaccagaa aaattcaact
    33961 aagctcaatg aaagaagaaa ttgaaactta cttgcatata tcaggtgtaa tcgtgcggat
    34021 caacgcaggg cgtattcaaa ttactttacc attaggagaa aatgatcgta ttccaattga
    34081 tgtaccatca atgatcaata gcgtgtttta gaagacatca caaatagaaa cagaaaatac
    34141 aaactaccag ttcacaaaca gaaaagtacc gagaaaagtg aggaatagaa atggctatga
    34201 aattattaga gaaagtcaaa aagagagccg aaaaagctga atctattttg ctagataaag
    34261 aacctaatat aaagcttaat ggaaaattca gtgtcaatat gttgaacaca aaggctaaag
    34321 acccaacaga tgctattttt ggaattaagc ctgatggcac aattgctcat ttttgcatca
    34381 gcgaaatgcc acacatgttg gttgcaggaa cgacaggatc agggaaatca gtattactga
    34441 atgaaattct tgttacagta atgtgtcact caacgcctga tgaattaaaa ataggaattg
    34501 tagacccaaa aagggttgaa tttggaagat acaaaaaatt gccgttcatg ttggctgatc
    34561 caattacaga tatggatgaa gcttatgatt tttttgaata tcttgtgatc ttgatgcatg
    34621 aacgttacaa actgatggaa aaagcagggt tacaaaacat cacgctatat aacgaatatg
    34681 ccgaaaaaaa tggattagat agatttccct atgtgatctt attagttgat gaatattcac
    34741 agttggttgg aacacataaa gaagtagaag gtttgattgt tcaattagga caaatggcaa
    34801 gggcagcagg tattcatgtt atcttagcaa cacaatctcc tcgatcaaca gttgtcactg
    34861 gtattatcaa agccaatttt ccgtcacgtg tttgccttat ggtagcaagt gatttagaaa
    34921 gtaggattgt attagatgaa ggcggtggag aaagtctaag tccaaaggga gatatgctta
    34981 ttaagcttgt tgatggaagt atggttagag cgcagggagc ttacatcagt aacaaagaaa
    35041 ttgaaacaat cttcaatcac cttagaaata ctatgccaga accagaatat gttgattacc
    35101 gagcaatcgt agctgaaact cgtggtgagg gcggtgaaga cgaggatttt ggtggaattg
    35161 gtaaagctcc tgtaagaaaa tctgctaaaa gaaatttgaa cacaccttta gcaaatccat
    35221 catctaaaga gccatcaatt gaaaagaaga gtgcgtttgc tattaagctt gaaaagaaca
    35281 aagaagaaaa agtcgagcag ggagctaaaa cactagaaaa actgcaagca gaaaaactag
    35341 aaaaagtttt aagggcatta ggaaaagctg atcctgaacc gcaaacctca aacaacacga
    35401 ataataaaca gccgattgca tctaaaacca aacctaaagc taatgatcca aaaggctcag
    35461 aaaacgtagt gatcacaact gataaatctg aaaagaaaca aataaatgga agtacttcac
    35521 ctatcatgca aaaaatgtta aaagcaaaaa aacaggtgca taaatgatca tcgtcagttc
    35581 ttttttaaga tggcttactc catataaata tacagatgaa gtagttgtga aacgagcgaa
    35641 gaaaagagat aaaaaagcgg tactaactcc taaacagatg cttgatcatg gcattttagg
    35701 ggtgaaggtt ttagaagtga ataaaccgtt tgaatcaaat agtgcaagag aaaagttgct
    35761 tggagctgaa gccgatgtcg catattttta ccatgacaaa agcaagcatc aaatagctat
    35821 taaattcaat ccaaaatcgg catggcggta tcaagcaaga gatgacttga aacgtaggaa
    35881 acttagacca atgctctatc cagaaggaaa agaagcattt gatagaaccc acatcgttcc
    35941 cattggtttt catggtacag aatcagataa ccgcctatta gtaggttgga acagcaatca
    36001 aaatagagtt cagatcaata aatttgagaa gaaagtaagg aaattcaacg atcgaaaaac
    36061 gatcctatgg tttgcagata tttgtaaaca gccggatgga acagcaaagt gggaagcggt
    36121 gatatttgac gaaaatggta aaaaagtaat gtctgaaaca tggcatgata aagctaaatt
    36181 tgtttggcag tagcagtaga ggtgaaaaag attgctagaa ctttttgaag caaatagaac
    36241 aggctttaat caaaagttag aagtgggaga aactatagaa tataaagata aaaactatat
    36301 tattatcgga atctataaca cgagaattaa cagaataggt gatccacgta taaccagtga
    36361 cttggtttgt caatctgtta actattcacc tgatctgaca agcaggtata agaaatacgt
    36421 gttattagaa tatagacaca agattactga tgaaattggt gtaaacaaca cacgaaacat
    36481 atttaagatc ggaacagtga ttccttatag taacaacgaa gaaaaaatat tttatcagat
    36541 ttacggaata gaaaagtttg aatatgaaca tgtcgatctg ctagtgacgt accaaatgcg
    36601 tttaatagaa ccttggtcac aggcagaaat tgataaagca gtaaaattga accgattatc
    36661 aaaatttaac gtattaggaa atgcctttta aaaatcgcaa tacacaaaca gaaaaatacc
    36721 aaaaaattag caatgaacgt aggtaagaac atgacaataa ataataataa ccctaaaaaa
    36781 tataatgctc ttgtccaatg ctatatcgaa aacaaaatta caatgcaaga agtcgctgat
    36841 gaattaggca aatcaaagag cctagtatca cacgatttta agcacaatgt ttttccgaac
    36901 ccgattgagc tgaggaaaaa atttcaaaag agagtttatc aagaaatagg taagtggatt
    36961 gatgaaggga atgcaattga ccctttaatt gaaaaatatc cttttttaaa aagcaaaaat
    37021 aaaattcaaa ggttaattcg tcaaggcgat attcaagcta aagaacctgt tttgacaacc
    37081 aatgatttgg ctaaaataaa tcttcataaa caaattattg aagactttga aaaaaatctt
    37141 tcaacaactg aaattttaaa aaaatacaat attccgagag gaaagctaac atcgactctt
    37201 tttagagaat atggagaatc ggcagttact aaagcaaagg aagtagcgaa tgagagaaaa
    37261 aaacaaaaat agtagtcgat acctagtagc gactgtagtt tttgtttgtg tattgatttt
    37321 cctgtttgat tttttgcaga ttggaaaaag cataattgga aatacattga gtgcaaagga
    37381 aatggttgct ttaggtggtt tgtatgaagg acacttgttc ccgaatatat tcatttctat
    37441 cgtttctcat attaacttgt accatttaat ttctaattta ttgttcttag tagttcttga
    37501 agaagcactg aatgatcagt atgagtggtt aacattattg atcgttttaa tcatatcagg
    37561 atttctagga agcttagttc cttttctgct acacagcaac atcgtaagta tggggcttag
    37621 tggaggtatt tttggatgga ttgctttatt tgcgattgca tacatccgtt ttgatagcaa
    37681 gaaacattta ttactaagtg tttttgtatt attgataatc ggcattttat tggtatctac
    37741 ttttgaatca ggcgaggcaa atgtcttgtc acatgtaatc ggatttgtct gtggaatgat
    37801 cttgggattt attctaaaga acgtttcatt gatcaaacaa aagaattaaa agggagtgct
    37861 aaaaatgcca aaaagaaaag aaaagaaaat tccagtgttt ttgtctgtct cagaaatgtt
    37921 atcacttcaa atactgttaa gaaaaaagat tgaaacaata gacgaagaag ttagaaataa
    37981 aagggctatg tacgaacaac gtgaagcgct aagagaacga atccactatt ttgatgatat
    38041 acacactaaa atctatgaag cacgtacaaa tcacgaagac gaaagtaaat taggattagc
    38101 tcaacaacgg atacttgaat ggcttaatac gaatactgat caagaaccgt taagagtgat
    38161 tagtaatctt tttgaatttg gaacgaagca ttggtttaat gctgtagaga aggatgctga
    38221 cttacaagaa gcgtttgata cgctaagtaa ggatagcaaa tccatgcgag atttattgat
    38281 tacttatcta aatgagcatg agtttgaaca aacgaaaacc aaaaagaaaa ataaagaata
    38341 gaaagttgtg atgaatcatg ctattaaccc tgttaggtta tttatgttta attcttgctt
    38401 tttttggggc aatcgctata attattggtc aatttcaaac aaataatccg attatgcgta
    38461 acataggcat tggaattgtg attgtaacct ttattgtgtt taatgccact aagatttttg
    38521 gaagtaacga taaacaattt ggtatatcgg gagttaataa tagttatgct ccagattctt
    38581 tgggaagggt agtaatagat ggaacatttg aaaatgtaac gagttataaa aatgtttcgc
    38641 tgacagtgaa ggaagatggc aagaaagcat cagacgacgt tctcgctact attaagccgt
    38701 ctcaatcaca atttgaagtg ttttatacag ttaaagagaa tagccctgta cacaagctga
    38761 cattggtttt taacgttgat ggagaagaat actataaaga tgtaaaaata aataaaacat
    38821 caacgaaaaa gattatcgaa tcagcgaaaa aagaagtagc tacagaagaa agtacaactg
    38881 aatcaagttc tactgtcgaa gcggaactac cagataaaat tgattttgat cctgaaaaag
    38941 tcaaatcgtt tgaatctgtt tctggttcta tggctgatcg gtttaacact gaagtcttca
    39001 aagctatcaa aactgttaaa ttggatactg aaaagcaaat gattaatgtt accttattca
    39061 attcagttga atctatggat caggataaaa aagttacggt tgcaagagaa ctaggaaaaa
    39121 gagtaattgg tttgtatcgt ggtactgcta aaccagattc agacggtgca gatttgccag
    39181 ttcatgtagt ttatgaagac ggtaaagttt ttggagattc tacagcaaac gaagcattaa
    39241 cgattaaatt ggctaaatag ccagaaagtt ataataaaca aatggtgtca caccaataat
    39301 atataggtgt gacaccttgg agatgatagc tgatggtagt aaataaagaa aaagttagaa
    39361 agtatgtgac tttaaaaata gcttctgtta aatatttaga aaagcgagct aaagaagaag
    39421 aaagagcaag aggtaagaaa gtaacagttt cagagttgtt agaagaattg atcgagaatg
    39481 atcaaacaat aagacgtgca tttactaata ggtctatcag ataacaatat aattaataaa
    39541 aaagaaagag tgatttggtc ttagactaaa tcactctttt tatataaagt ttctttacac
    39601 tcattccgaa taatgttaaa attctttata tgatatatag ttccttgtca gaggaagaga
    39661 taaccagcaa tattggaggg ataataagtg gaaaacaata atgatattag acattttagc
    39721 attggggaaa gagttacaac agtaagtgaa ttatcaaagg attatagaga taaagaggga
    39781 acgattattt ctcttaataa gaatcgaaga ggcgtttggc aatatgttgt tgatttaggt
    39841 gatggcgtta cagttgagtg taaagatagt caactgatca gaaataatgg cagtcattcc
    39901 attgaaaaag caattcttgt atacccaaaa gtatcaagta agatcatgaa tgactggttg
    39961 cttggaaaat ttggtgagta caataaaaaa gaaatcgcag aagtgatcgg caaatcttat
    40021 ccaacagtat tagggtatct aaaaaagata acgagtaaag aagcaaaaat tgaggatgtt
    40081 ttaacagtaa gagagcttca atcattaatc gagtatcgga ataaacaaat aacaaatcct
    40141 gagtacctta gaaaagaaga tggtctattg aaacaattgg aacaagtaaa taaaatccgg
    40201 aagatgcaaa aggtagctcc tttaagcctt gataccttta aaaaaatcta tttgaaagag
    40261 atcaaaaaag actaatgtgg actgtaactt taccgttcca acctgtatct gcaccaagac
    40321 caagtgcaag aaggaatgct gctgatgaaa ctaatgacgt agcagagaaa aggatttcaa
    40381 cctactatga taaaaagtat cttgattatt tgaaaaaaat aaagagcttt atcgaagatc
    40441 aagatttatt agatgatgaa ttttactcga ttgtaaatga tcctatgggc gcaattatgg
    40501 aagtggactt ttactaccag ataccaaaaa gctataaaaa agtctataac attatgaaga
    40561 caacagcgcc agatttagat aatttagtaa aaagtgctat ggatggtatt tttaacatct
    40621 cagctatacg agatagccat atcacaggct taattgcttt taaatataat gtggcgaacg
    40681 aaggaaaaac agttgtgagg atcaaacgat attcagaatt tgagtgggat acttctgaag
    40741 gattgaacgg agatatatgg gaagctacat ttgattttaa accagtagca acgccaagac
    40801 caaagtctaa atttagagga aatgggatta tcacttacta tccaaaagag tacaacgact
    40861 atttggaaaa tatcaagaat acgcttaaag agaaagattt ggtaaatgat caactgaaaa
    40921 aggtaatgaa tgctcctatg ggggttatag ctcagataga ttatttttac caagtgagaa
    40981 aagataagaa aaagcttgat tcactgatgc gtacttcaca gccggatatt gataatttag
    41041 tcaaaggaac attggacgga atttttaaca aggaaatagg catcaaagat agcagggttg
    41101 tagggttaat tgcttttaaa tacaatactt ttgaaaagtc gaaaacaaac gtgagactac
    41161 aggagatggg ctgatgaata ataatgaaaa aattcttcat gtacttgata gctttgaaat
    41221 aattcaagaa gaactaaaaa aatatcggga tgttttggaa caaagatatg attttgtcaa
    41281 tcagcaaaag agtaatcaca tggatttcat tctcaatatg aatgacttga agaagaaatt
    41341 agttgaacga aaagaacaag aaaagttgat caaagcttac tttgaactcg gtgaaaaaga
    41401 agttaaaaat gcaatggaac tgaatgaaga gcgcagggtt ttagatcagc tgttagaaca
    41461 attattggtg atgtttcaaa aaggacgaat agatgaggat ttaattgaag aaggattgag
    41521 aaaatatcct tcaaactcag gtatcggaat agtgctgaaa gcgattgatg aagaagaaat
    41581 tgaagctttc ataccgccag aggattttga atcggcaatg gaatatataa aatattatag
    41641 ccaagggatc actgctttta gagagtttga tcctgaagat gttattcatg acttgaacaa
    41701 cctaaaagaa tggtgtgaaa gttatggagt cgatgattcc ggattggact acttgatcag
    41761 tattatggaa atcgaagaag agatgccgga taagcctgat ccaacagata ttttagaatt
    41821 gatccatgaa gctagaaatc caattgccta tatttctaga ggatatactg tattggaata
    41881 ctataagcct tatatatcag cgatgaacca tttaagacgt gtattaaggg aaaaacgtga
    41941 ataccgctca gtgttgaatg caacgaatag attagaaaaa gctgtttcag agctagatgc
    42001 ttattatcgt gaacattacc tgcaagcagg tggtatgcca agaaatacga aagcaaatat
    42061 cagtaggtat ataaaaaaag ctgagtaggc ggagttagcc cataaaatgg caaaaggtaa
    42121 cctgaaaggg gcattttgaa tgaaaaggga tgtttttgaa ttgtacgtat tagaacgtct
    42181 agaagacggc atgatggcac aagagataat tgatgaaatt gatgatatga tcagcagatt
    42241 tggtcaattg gagttgaatg atggaaacaa aaattcggta gtgctaaaac aattttggtt
    42301 gaacaatttt tcgtataagg attttatcaa agaaaaggca aatacattgt actgactaaa
    42361 ttcacgagga gcagtgatga gagcttattg ctaagagcag ttaggctctt ttttgtttgt
    42421 gtaaaattat ttgttttata atacacattt atacacaaaa aaatattgac agatagtaca
    42481 caaatgtggt aggatatatt tgtaagcaag ttagattaac aagttaaagg aagagcagtt
    42541 atgaaaaaag aagaactaat agatatattt caaattgttg aacgagcaaa taatatggga
    42601 attatgtttt ttgatcgtat ttctttaaag atgg
//
LOCUS       DACCXO010000014        41267 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.014, whole genome shotgun sequence.
ACCESSION   DACCXO010000014 DACCXO010000000
VERSION     DACCXO010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.014"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            79..1102
                     /locus_tag="IVY70_000697"
                     /pseudo
     CDS             79..1102
                     /locus_tag="IVY70_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            1445..1701
                     /locus_tag="IVY70_000698"
                     /pseudo
     CDS             1445..1701
                     /locus_tag="IVY70_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2088..2315
                     /locus_tag="IVY70_000699"
     CDS             2088..2315
                     /locus_tag="IVY70_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP7957852.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            2331..3497
                     /locus_tag="IVY70_000700"
     CDS             2331..3497
                     /locus_tag="IVY70_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP7957853.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGNRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            3732..4775
                     /gene="hrcA"
                     /locus_tag="IVY70_000701"
     CDS             3732..4775
                     /gene="hrcA"
                     /locus_tag="IVY70_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP7957854.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            4869..5432
                     /gene="grpE"
                     /locus_tag="IVY70_000702"
     CDS             4869..5432
                     /gene="grpE"
                     /locus_tag="IVY70_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP7957855.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            5483..7312
                     /gene="dnaK"
                     /locus_tag="IVY70_000703"
     CDS             5483..7312
                     /gene="dnaK"
                     /locus_tag="IVY70_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP7957856.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            7463..8629
                     /gene="dnaJ"
                     /locus_tag="IVY70_000704"
     CDS             7463..8629
                     /gene="dnaJ"
                     /locus_tag="IVY70_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP7957857.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            8791..8952
                     /locus_tag="IVY70_000705"
     CDS             8791..8952
                     /locus_tag="IVY70_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7957858.1"
                     /translation="MIIIVSEGGIDYVLDIVNEPMVLSYAMEQVERLLVCAGEQAMRL
                     VQLSEKTRN"
     gene            complement(8997..9734)
                     /locus_tag="IVY70_000706"
     CDS             complement(8997..9734)
                     /locus_tag="IVY70_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7957859.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(9736..11238)
                     /locus_tag="IVY70_000707"
     CDS             complement(9736..11238)
                     /locus_tag="IVY70_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7957860.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            11497..13491
                     /gene="uvrB"
                     /locus_tag="IVY70_000708"
     CDS             11497..13491
                     /gene="uvrB"
                     /locus_tag="IVY70_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP7957861.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            13501..16317
                     /gene="uvrA"
                     /locus_tag="IVY70_000709"
     CDS             13501..16317
                     /gene="uvrA"
                     /locus_tag="IVY70_000709"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP7957862.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            16580..17845
                     /locus_tag="IVY70_000710"
     CDS             16580..17845
                     /locus_tag="IVY70_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP7957863.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            17849..18580
                     /locus_tag="IVY70_000711"
     CDS             17849..18580
                     /locus_tag="IVY70_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP7957864.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            18715..19599
                     /gene="rapZ"
                     /locus_tag="IVY70_000712"
     CDS             18715..19599
                     /gene="rapZ"
                     /locus_tag="IVY70_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP7957865.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            19596..20594
                     /locus_tag="IVY70_000713"
     CDS             19596..20594
                     /locus_tag="IVY70_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP7957866.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            20628..21563
                     /gene="whiA"
                     /locus_tag="IVY70_000714"
     CDS             20628..21563
                     /gene="whiA"
                     /locus_tag="IVY70_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP7957867.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            22131..22787
                     /locus_tag="IVY70_000715"
     CDS             22131..22787
                     /locus_tag="IVY70_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957868.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            22966..23531
                     /locus_tag="IVY70_000716"
                     /pseudo
     CDS             22966..23531
                     /locus_tag="IVY70_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24230..24751)
                     /locus_tag="IVY70_000717"
     CDS             complement(24230..24751)
                     /locus_tag="IVY70_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP7957869.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            24949..28629
                     /gene="nifJ"
                     /locus_tag="IVY70_000718"
     CDS             24949..28629
                     /gene="nifJ"
                     /locus_tag="IVY70_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP7957870.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            28818..29705
                     /locus_tag="IVY70_000719"
     CDS             28818..29705
                     /locus_tag="IVY70_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP7957871.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            29702..30871
                     /locus_tag="IVY70_000720"
     CDS             29702..30871
                     /locus_tag="IVY70_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957872.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            30895..32250
                     /locus_tag="IVY70_000721"
     CDS             30895..32250
                     /locus_tag="IVY70_000721"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP7957873.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(32322..34127)
                     /gene="glmS"
                     /locus_tag="IVY70_000722"
     CDS             complement(32322..34127)
                     /gene="glmS"
                     /locus_tag="IVY70_000722"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP7957874.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            34537..34827
                     /locus_tag="IVY70_000723"
     CDS             34537..34827
                     /locus_tag="IVY70_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957875.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            34840..35865
                     /gene="sppA"
                     /locus_tag="IVY70_000724"
     CDS             34840..35865
                     /gene="sppA"
                     /locus_tag="IVY70_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP7957876.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGASGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            35868..36527
                     /locus_tag="IVY70_000725"
     CDS             35868..36527
                     /locus_tag="IVY70_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP7957877.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            36684..37616
                     /locus_tag="IVY70_000726"
     CDS             36684..37616
                     /locus_tag="IVY70_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957878.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            37770..39059
                     /locus_tag="IVY70_000727"
     CDS             37770..39059
                     /locus_tag="IVY70_000727"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP7957879.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            39306..40556
                     /gene="clpX"
                     /locus_tag="IVY70_000728"
     CDS             39306..40556
                     /gene="clpX"
                     /locus_tag="IVY70_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP7957880.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            40639..41226
                     /locus_tag="IVY70_000729"
     CDS             40639..41226
                     /locus_tag="IVY70_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP7957881.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
BASE COUNT        13684 a         7059 c         8971 g        11553 t
ORIGIN      
        1 ttgacacaaa atgagttttt ttcgattttt caagttatct aatgcaatta tattgatatc
       61 attaggagct gcattgttat gaatccaaca ttgattacta agaaagaatt actaaaaaaa
      121 ttagatattt ctactggtgt attagctaat ttaattagaa atggcatgcc aaaagaggga
      181 gaaatgttta atcttgataa gattattaca tggcgagaaa attggtctaa aaacatatta
      241 ggtgagttag aagttggtag agtatatact aacaaagaaa tttcagagaa gtttaaatgt
      301 tccaaacagg gtggaatgag aagatctcat caaactaata ctcttgtact gtttagtgat
      361 caaacaggaa gtaatgttta taaagataag tggttaaatg gtattttaca atataccggt
      421 atggggctaa aaggggatca agtattggat aagaatcaaa ataaagtttt agccaattca
      481 aaaagtaatt ttgtaaagat tcatttattt gaaacattta aaccaaaaga acacacatat
      541 ctgggagaag tatatttagc aggacaaata tatactgtta atgaaaaaga tagttcgggc
      601 aacagcagaa aagtatataa atttccatta gctttaataa atcaagagca attgattgaa
      661 gataaagata tataaccagg aagaaaatca aactagacat attcgtaatt tatctaatgc
      721 taaattagag gaagaagcaa gaaaagttag taattataat aaaaaaatgg ctgcaaaata
      781 tgttaacgaa gaggagtttt gcttctcaaa aacaaaaata tataaacgta atcccttcgt
      841 atctgaatat gttaaacgac ttgcaaaagg tatttgtcaa ctttgccaag aaaaagggcc
      901 atttataaaa gacggtgtac cttatttaca ttgtcatcat atagaatatt tgtcgcaagg
      961 tggaaaagat gtgattgaaa actgtatcgc gttatgtccc aattgtcatg ctagaattca
     1021 tgaacttgag ttacctgacg ataaagaaaa attgctgaag atcgttgaaa aaagagaaaa
     1081 tagcttttct aaaataccgt aatttatttt tataggacaa ataagctaat gtgagctatg
     1141 ccttctatat aagactaagt tctattataa gtaacgaagt aattctcgtt ccttcctgta
     1201 gtaatatttt ttaaataacc tataggcttc ttcaaaagta catcctccag taaattctaa
     1261 gtactggagg attctttttc tagtgttgct ttactcatga aaaagtagtt ggctacttca
     1321 tcaaaatttc tgaacttttt taatgtataa taaagagctc gatcatttac tacaaggatg
     1381 ctagctccta cattttcaca gttttttagc aagtaaattt ttctaaaatc acatcaggaa
     1441 atgcatgaga ttctttaaga gaggcaatta tagggctatg attacggttc gtgtaagtga
     1501 cgccaaaaaa gaatggcttt cttatatggc tgaattttat gggattactt tatctgaact
     1561 tctaaaaaac tattcgatgg aacaactaga agatgaatac aatcgtcaaa cggctgaaat
     1621 tgcccataaa cattggataa gaagtgggaa aacaacagta ttcatggaag aatccttaat
     1681 gaatttggtg ggctggaatg acctgccgtt taaaattcac gcccgaaatt cagaagcagc
     1741 tctgattggc aaatacgtgt attggtagag atagaagata atcaattcgt tgttatcgct
     1801 attcaagtag ggcaccgaag aaatgtctat gattaagaga ctgtttattt ttacaaaaaa
     1861 ataataattg gaatctagtt aaaactcttg aagtgagaga tttgactaga tttttttatg
     1921 aataaaataa ggacaaaatt gtttaggaag aatcatgagt gtaaaaaaat catccaagaa
     1981 gtcgattctt ggatttttta gtggatttgt tcgccaaata cgtttagtat gcgtatatga
     2041 gaggttgtaa gcaaagcgtt gtggtctgag cacttaagga ggaatctgtg aaaatagctt
     2101 ttcatatttt tgcagatttt gacttaatgc cgaaggtcag ctttgcgatt accgtttatc
     2161 gagaggctgt aaaagcaccg tgtagctccg atgacaataa gcaagcattt ttagagctag
     2221 gtttccttga ttctaaatat gcggcttatt tcaatgagtt ggtgcttgaa cgcgatttta
     2281 agaaggtcga aaacaggcac gacttttcga cgtaaggaga aagaatttca atgatagaaa
     2341 caataaatcc cgcagaaaac aaggtttctg cgtacattca tatcccattt tgcgagcata
     2401 tctgctatta ctgtgacttt aataaagttt ttttagaagg gcagccagta gatgagtatg
     2461 tggaaatgct gctgaatgaa atgagattaa tgctggaaag tcatcctaca gcgaatttgg
     2521 aaacacttta tgtaggaggc ggaacaccaa cttctttatc tgctaaacaa cttgatcgac
     2581 tattatcagg tgctagagag atcctgcctt ttcaaaatgg taatgagttt actgtagaag
     2641 cgaatcctgg tgatttgacc cgtgaaaaac ttcaagtgat gaaaaactat ggcgtcaatc
     2701 gtttatccat gggagtgcag acatttgaca atcgcttatt gaaaaaaatt ggacgtaaac
     2761 atactgcaga agatgtgtat caaacaatgc aatttttaga agcagaaaat tttaccaatg
     2821 taagcatcga tttgatttat gcattaccgg gacaaacact agaaggaaat cgggatactt
     2881 tggaaaaagc tttggctttg gatctgcctc actattcttt gtactcattg atcctcgaaa
     2941 acaaaacaat gttcatgaac tgggtacgac aaggaagact cgaactgcct gatcaggaaa
     3001 cggaaaccag aatgtttgaa gagacgattg aagcaatgga aaagcatgga cgtcaccaat
     3061 atgaaataag taactttggg ctctccggac atgaatctca acataacctc atgtactgga
     3121 acaatgacca ctattttggg tttggagctg gtgctagcgg ttatcttgga gatattcgtt
     3181 accgcaacaa aggaccaatc caacactatt tacgcccatt acgagcaaat gaacgcccgg
     3241 tattggaaga agaaaaattg acacgaaaaa atcaaataga agaagaaatg ttcttaggac
     3301 taagaaaaaa aacagggatt actaaaaaac atttctatga tcgctatcaa aaaacaattg
     3361 aatcggtgta cggtgatgtg ctaagggaat tagagcaaga agggttactt gttaatgaag
     3421 aagaccgtgt ttatttgaca ccaagaggga cttttttggg gaacaatgtg tttgagcgct
     3481 ttttatttga aaaatagttt gaagaatatt tgttttttat cgaaacttga aaagaaaaag
     3541 tttagaaagt ttgacaatga ggtattgaag tattgatata actggattta catcaattat
     3601 ttagaattag cactcttgtg tttagagtgc taaaaaatgg agtctttctc ttgacaggag
     3661 aagggcttct tgctatattt ataagtgaat tagcacttag aatatatgag tgctaatgag
     3721 aggtgaggaa gatgttaaca aagcgacaaa acgacatttt acgtctgatc atccaacact
     3781 atacgagtag tggcgtgccc gttggttcca aaacattaat gtctgaggga gtcaaggcca
     3841 gctcagcaac gattcgcaat gatatgaaag cattagaaga tgaaggattg ctgttgaaga
     3901 ctcactcttc ttctgggcgg attccatctg ctttaggcta tcgttattac gtggatcatt
     3961 tgttaaaacc tgctcgcgta gcagaagatg atttgctgct gattcgtcag tctctaggca
     4021 gagaatttca tgaaatcaat gagattatta agcaatctgc tgagatatta tctgagctga
     4081 caagctatac gactttttca ttgggacctg aaataaaaga ccgccgactg actggctttc
     4141 gtatcgtacc cttgaataat cgacagatta ttgctatcgt cgtaacagat atggggaatg
     4201 tagaaagcca agtgttcact cttcccgaat atatgggaag tcaggaacta gaaaaaatgg
     4261 tacagattgt aaacgaccgt ctagtcggag atcctctgat gacggtgtac cataaacttc
     4321 gtacggagat tccgatgatt cttcataaat atttccagac gacagaaggg atgtctcagt
     4381 tatttgacac cgttcttggt catgtttttg aagaaaaggt ttatgtcagt ggacaaatga
     4441 atctcttgga ttatgagccc cgtcaagatc ttgatcagtt caaatcgatg ttttctttta
     4501 tgaaagactc agacggactt acacaaatga tcactccgat ggatagcaat attcatatca
     4561 agattggtgc tgaattaggc aatgatttat tgcagaatat gagtatgatt caagcaagtt
     4621 acgagataga tggtcatgga agaggtacaa tagccttgtt agggcctact agtatgcctt
     4681 attctcggat gttcggtcta gtcgatgttt atcgaagaga actagcagct aaacttgctg
     4741 attactaccg ctcgcttgac ttgtcaaact cgtaatgtga ataacgaagc tatttgcagc
     4801 tgatcgttct ctctttctta gtaagagcaa tcaaacagct gactgaataa tgaaaggaag
     4861 actgtatcgt gaaagaaaat caagaagaac tagataaaga aatgacagat gctcagccag
     4921 aaccggaaat cgatgtcgaa gctgcagaag attcgggtat ttcagaagct gaagcggaag
     4981 aattcgaaac agcgaaatta aaagctgagt tagaagaaat ggaagataaa tacttgcggg
     5041 ctcgtgctga aatcgcaaat atggcaaatc gaggaaaaaa tgagcgtgag caattacaaa
     5101 aataccgttc acaagattta gccaaaaaat tacttccatc aattgacaac ctggaacgtg
     5161 cattagccac agaagtgtca gatgatcaag gggcaggttt gaaaaaaggc gtggaaatgg
     5221 tcttggaaag tttgcgtaac gcactggaag aagaaggaat tgaaaaaatc cctgctaaag
     5281 gagaagcctt tgatcctaat cttcatcaag cagtacaaac tgttccagcg actgaagata
     5341 caccagccga tacgattgta gaagtactac aagaaggcta caaattacat gatcgcgtgt
     5401 taagaccaac tatggtcatt gttgcacaat aattgtgcgg atataaaact tacactttca
     5461 attaaagaag gagacgttaa ttatgagtaa aattatcggt attgacttag gtacgactaa
     5521 ctctgcagta gcagtattgg aaggaaacga agcaaaaatc atcgctaacc cagaaggaaa
     5581 ccgtacaaca ccttctgttg tatcattcaa aaatggagaa atccaagtag gggaagttgc
     5641 aaaacgtcaa gctgtgacaa acccaaatac gatttcatca atcaaacgcc atatcggtga
     5701 agcaggttac aaagtagatg tagaaggtaa atcatataca ccacaagaaa tctcagcaat
     5761 gatccttcaa tatatcaaag gattcgcaga agactattta ggtgaaaaag tcgaaaaagc
     5821 agttatcact gtgccagctt actttaatga tgcacaacgt caagcaacaa aagatgctgg
     5881 taagatcgct ggtctagaag tagaacgtat cgtcaatgaa ccaactgcag ctgctcttgc
     5941 ctatggttta gataaaacag atcgcgacga aaaaatctta gtattcgacc ttggtggcgg
     6001 tacttttgac gtatctatcc ttgaactagg agacggtgtc tttgatgtat tgtcaacagc
     6061 tggcgacaac catctaggtg gggacgactt tgataacaaa atcatcgact acatggtagc
     6121 agaattcaaa aaagaaaaca gcattgactt gtctcaagac aaaatggctt tacaacgctt
     6181 gaaagacgct gctgaaaaag cgaaaaaaga tttatctggc gtatcaagca cacaaatcag
     6241 cttgccattt atcacagcag gagaatctgg accattgcat ttggaaatga cattgacacg
     6301 tgcaaaattt gatgaattaa cagctgactt agtagaacgt acaaaagtac cagtacgtca
     6361 agcgttaaaa gatgcaggtc tgtcacaatc agaaatcgac gaagttattt tagtcggtgg
     6421 atctactcgt atccctgcag ttgtagaagc agtacgtaaa gaaacaggaa aagaaccaaa
     6481 caaatcagtt aaccctgatg aagtagttgc tatgggtgct gctatccaag gtggcgtgat
     6541 cactggtgat gtgaaagatg ttgttttact tgacgttaca ccattgtcat taggtatcga
     6601 aacaatgggt ggagtgttca ctaaattgat tgaccgcaat acaacgattc caacaagtaa
     6661 atcacaagta ttctcaactg cggcagataa tcaaccagca gtagacatcc atgtattaca
     6721 aggtgaacgc ccaatggctg ctgacaacaa aactctagga agattccaat tgacagatat
     6781 tccagcagct cctcgtggta ttccacaaat cgaagtaaca ttcgatattg ataaaaacgg
     6841 gattgtaaat gtatctgcga aagatctagg tacacaaaaa gaacaaaaaa tcacgatcaa
     6901 atcttcttca ggtctaacag acgaagaaat cgaacgtatg gtgaaagatg cagaagcaaa
     6961 cgctgaagca gataaagctc gtaaagagga agttgaccta cgtaatgatg tagacgcatt
     7021 gctattctct gtggacaaaa cattgaaaga attagaaggc aaagtggacg aagaagaagt
     7081 gaaaaaagca gaaacagctc gtgacgaatt aaaagccgct gttgaagcaa acaacatcga
     7141 ggaaatgaaa actaaacgcg atgcattgaa cgaaatcgtt caaaacttga ctgttaaact
     7201 atacgaacaa gctgcacaac aacaagctca agaaaatcca gaagcagctc aaggcggagc
     7261 agatgatgtc gttgatgctg attttgaaga agtcgatggc gacgataaat aatcagttca
     7321 ctgaatttcg agagaaggcc aaagttgcta ctttggcttt ttcttttgaa aaatagtttg
     7381 aagtatcggt ttttgtttca aacaaaatat gatatgataa caagcgacgt gaaaatgatg
     7441 agaaccaata ggaggaaagc ctatggcaac aaaacgtgat tattatgaag tcttagggct
     7501 ctcaaaagga gcatcagaag atgaaataaa gaaagcttat cgtaagcttt cgaaaaaata
     7561 ccatcctgac atcaataaag aacctgatgc ggaagaaaaa ttcaaagaag tatcagaagc
     7621 atatgagatt ttaagtgacc cgcaaaaacg cgctgcatac gaccaatacg ggcatgcagg
     7681 cacagatcca aattacggtg ctggtggagc tggtggcgga tttggtggat tcggcggctt
     7741 ctctggtggc ggcttcggtg gttttgaaga tatcttcgac tctttctttg gaggaggcgg
     7801 tcggtcagtc gatccaaatg cgcctcgtca aggtgctgat ttgcaatata cggtcgactt
     7861 gtcttttgaa gaagcgattt ttggtgtaga aaaagaaatc aagtataatc gggaagaaat
     7921 ctgtcatact tgtggtggaa acggtgcaaa accaggcaca caaccaacta cttgtcataa
     7981 atgtcatggt gcaggtacga tcaatgtaga acgccaaacg ccacttggac gagtgatgag
     8041 ccgtcaaact tgtgatgttt gtcatggcac agggaaagaa atcaaagaac cttgtccaac
     8101 ctgtcatggc tcaggacatg agaagaaagc acattcagtt aaagtgaatg taccagctgg
     8161 tgtagaagaa ggacaacaaa tgcgtttagc tggtcaagga gaagctggag aaaacggcgg
     8221 accatttggt gatttgtatg tggtttttcg tgtagaagaa agtgatatct ttgatcgtga
     8281 aggttctgaa atttactatg aattgccttt aagttttgta caagcagctt tgggggatga
     8341 agtccaagta ccaactgtcc atggagatgt gaaattgaag atccctgctg gaacacagac
     8401 aggtacgaac ttccgtttac gtggaaaagg cgctccgaag ttacgtggtg gtgcaacagg
     8461 cgaccaacat gtcaaagtaa aactgatcac accgaaaaat ctaaatgaac aacaaagaga
     8521 agcattacgt gcattcgctg aagctggcgg catcaaagtt gaagaacagc aagaagacgg
     8581 tttcttcgat aagtttaaag atgcttttgg cgggaaaaag cggaagtagt ttttctagta
     8641 aaaatataaa gtagacttgc ccaaattttc tatttctagt aaaaacgcta agtcattaat
     8701 aattaaaaac cagacgaaca atcatacgtt cgcctggttt ttttgtagtt gtcatgtttg
     8761 taccaacaaa aaagccagct ttagaataag ataatcatca tagtttctga aggtggtatc
     8821 gactatgtac ttgatatcgt caatgaaccg atggtacttt cttatgcgat ggaacaggta
     8881 gagcgattgc ttgtttgtgc aggagaacaa gcgatgcggc tggtacagtt aagtgaaaaa
     8941 acaagaaact aaaaatgccc ggtcgtcatg aagcgaccgg gcatttttgg tttttattaa
     9001 atatttaata ctttattcaa gaaatctttt gtccgttcat tttgcggatt ttcaaagatt
     9061 tgttgaggcg ttccgtcttc taaaaagttt ccgtcgtcaa tgaacatgac acgattggcc
     9121 acttctttag cgaagcccat ttcgtgcgta acgataacca tcgtcatacc ttgttctgct
     9181 aactttttca tgacgttcaa gacatcaccg accatttcgg ggtcgagtgc agatgtaggc
     9241 tcatcaaaaa gcatgatgtc agggttcatc gctagagcac gagcaatagc cacacgctgc
     9301 ttttgtccac cggaaagcat ttcaggatac atatcttttt tatcttctag accaactgtc
     9361 tgcagcaatt tgattgcttt tttctctgct tcttctctag attctttctt caggtccatc
     9421 ggtgctaaaa tgatgttttc caaaatagaa agatgaggga ataggttaaa gtgctggaat
     9481 accattccga tatgttgtct caccaagttg atatctgtgc ttttatccat caagtgggcg
     9541 ccgtcaataa taatttcacc gtcagaaggt tcttctaaac gattcaagca acgcaaaaat
     9601 gtactttttc ctgaacctga agggccaatg atacagacga catctccttc gttgatggac
     9661 acattgatgt cgtttaatac tgtgttatcg ccgtattttt tgactaaatg ctcaactaag
     9721 attttttctg ccatcttact tcaccttctt ttctaaaact tttgctaatt ttgtcaatgc
     9781 tgtgatcaaa atcaagtaca tgatcgcaat gatcaagtaa acatacgtac tttgtgttgt
     9841 acgtgcaaca atgatcttac cagtttgcag caattcgact acaccgattg cggaaatgat
     9901 cgttgtgtct tttaatgaga taacgaactg attgacgaat gatgggatca tgatgcgtat
     9961 cgcttgcggc aaaatgattt tttgcattgt ccgattataa gaaagaccta aacttctgga
    10021 agcttccatt tgtccgacag gaacggcgtt gatcccgcct cgaacaatct ctgcgatata
    10081 agcacttgcg ttaagagtca aggtgataac accagcaagg aagtcaggaa tcgtaaatcc
    10141 aacgatacca ggcaacccaa agaaaataaa gaacgcaaga accatcattg gaattcctcg
    10201 gatgatatcg acataaatac tagcaattgt gcgaagagcg cgtattggtg ctacactgaa
    10261 taatccaaaa atcacaccga ttactatagc caaagcaaag gagatcaaag ttaggagaat
    10321 tgttctccaa agtcctttca acaatgcttg atagttgttt tggataagtc caagaaatgt
    10381 tgattcatca gcagtggaag actcactgga agtttctgaa ccatccgcta catattcttt
    10441 aataatctta tcgtattcgc ctgttctttt taattctttc aatccttcat tgaacatatt
    10501 aagaagctca ggattttgtc cttttttaac ggcaaatgca tattttccgc cagattcttt
    10561 tttgatcggt gtttccaatt cttgtccttg tttgatcgca taaccgatta ccggataatc
    10621 atccatcatt gcatcgattg cgccaacttt taacccgtcg tacaattgat cagcagcatc
    10681 aaaatatttg atagaatagc catattcaga ttcatggtcg cttaaaaatt cagcactttc
    10741 ggtaccaact tttgctccga ctgtttttcc tttcaaatct tcataagact tgatcgaagt
    10801 atctcctttt ttgacagcta attggatccc gctatcaaag taagaatcag aaaaatcata
    10861 gcttttcttt cgttcatctg ttacagtcat tccggcaatc attccgtctg cttgtcctga
    10921 ttccaaagct tgcaaagcag cagagaaacc acggtagtcc attcggatgt taaatccttg
    10981 cagtttggcg atgttttcca tcaattttac atcgatgcct tcgtactcgc cctttgcgtt
    11041 ttgaaattca aatggagcaa aagcagagtc actggcaatt acgtatacgt cttttttggg
    11101 ctctattttt tgcattgtct cgcttgtgct tgactctgcc gttgtagtgt tgtcagcttg
    11161 tgcaacatgt ccaaacgcaa gtgtcacaag tcctgtcatt aagaaaacaa atgagtgaaa
    11221 taaatgcttt cttttcataa tcaacctcct aaatttccaa tattttatta ttataggcga
    11281 aaagtgctga atcagacaag actgtaagct cgaaaaaatg aaaaaaatgc gagtttctaa
    11341 cattttatgt tataaaacgt aacatgcata ataagataaa aaagtggcga aagtatgttc
    11401 ggtgtggtat aattttttag taaggaatgc ctttgctttc cttttctttt tattggtcgt
    11461 aaaatgaaaa ggaatgaatg aaaaggagga cgaagaatga ttgagagaga tacctcccga
    11521 cactttgagc ttgtctcgaa ataccaaccg gctggtgatc agcctgaagc gattaaccaa
    11581 ttagtggatg gcgtagtcgg cgggaaaaaa gcacaaatct tattaggtgc gactggaaca
    11641 gggaaaacat atacgatttc caatcttatt gagaaagtga ataaaccaac actaattatt
    11701 gcccataaca aaacattggc tggtcagctg tacggggaat tcaaggaatt tttccctaac
    11761 aatgctgttg aatattttgt ttcttattat gattattatc agcctgaagc ttatgtgcct
    11821 tcaagcgata cgtatatcga aaaagattca agtgtaaatg atgagatcga caaattacga
    11881 cattcggcta ctagttcatt attagaaaga aatgatgtga tcgtcattgc atccgtttca
    11941 tgtatttttg gattaggttc gccttttgag taccaaaaac aagtagtttc gattcgacag
    12001 ggagcagaac tagatcgcaa tcagttgatt cgtgacttag tcagtatcca attcgaaaga
    12061 aatgatatag attttcagcg cggtcgtttt cgagtgcgcg gtgatgtcgt ggagattttt
    12121 ccggcttcaa gagatgaacg tgccttacgt gtggagtttt ttggagatga ggtcgaacgt
    12181 atccgagaag tcaatgcact gacaggtgaa gttttaggag aaacggaaca cgtggcaatc
    12241 ttcccggcga ctcactttgt gacgaatgat gaacatatgg agcatgccgt agcgaatatc
    12301 aaagcggaat tagaacagcg tttaactgtc ttgcgaaatg aaaacaaact tctggaagct
    12361 caacgtttag aacagcgaac caattacgat attgaaatga tgttagaaat gggctacacg
    12421 tcgggaattg aaaattattc tcggcatatg gacggacgta aagaaggtga accgccttat
    12481 acgctattgg atttctttcc tgaagatttt ttgatcgtgg cggacgaatc gcacgtaacc
    12541 atgcctcaag tcagaggaat gtataatggt gaccgagcga gaaaacagat gctagtagat
    12601 tacggtttcc gtttgccatc tgctttagac aatcggccac ttcggttaga agagtttgaa
    12661 aaacatgtca atcagatcat ttatgtctct gcgacgccag gaccatatga acatgaacaa
    12721 acagataccg ttatccaaca aatcatccgt ccgacagggc tgcttgaccc agtgatcgaa
    12781 gttcgtccaa tcatgggaca aatcgatgat ctagtaggcg aaataaatga acgagtcgaa
    12841 aaagaccagc gggtgtttgt aacgacattg acgaaaaaaa tggcagaaga cctcacagat
    12901 tatttcaaag aattagggat caaagtcaaa tatttgcata gtgatatcaa aacgctggaa
    12961 cgaacagaaa tcattcgaga tcttcgatta ggtgaatttg atgtgttagt tggaatcaat
    13021 ttgcttcgtg aaggattaga cgtacctgaa gtttcccttg ttgctatctt ggatgcggat
    13081 aaagaaggct ttttacgtag tgaacgttcc ttagtccaaa cgattggacg agctgctcgg
    13141 aatgaagaag gtaaagtaat catgtacgca gacaaagtaa cagattctat gcgtctagcg
    13201 atggatgaaa catcaagacg ccgtacgatt cagcagaagt acaatgaaga acatgggatc
    13261 gttccaaaaa cgatcattaa agaaatccga gatttgattt ctattacgaa agaatcggaa
    13321 gatgatacaa aagaagcggt acaagtctcg tatgaagaaa tgacgaaaga agaaaaagat
    13381 acgttattga tgaaattaga aaaagaaatg aaaaatgcgg cgaaagcatt ggattttgaa
    13441 acagccgcta atgtgcgaga tatgatttta gaattaaaag cttcaaagta ggaggacaac
    13501 atggctaacg ataaaattac gattcacggt gcacgtgccc ataacttgaa aaatatagat
    13561 gtaacgattc cacgagataa attcgtggtc gttacaggtt tgtccggttc tggaaaaagt
    13621 tccctagcat ttgatacgct gtacgctgaa gggcagcgga gatacgtaga gagcctttct
    13681 gcttatgctc gtcagttttt aggacaaatg gataagccgg acgtagatag catcgacgga
    13741 ttgagtccgg cgatttctat tgatcagaaa acaacaagta aaaatcctcg gtcgacagta
    13801 ggtactgtga cggaaatcaa tgactattta cgcttgttgt ttgcacgtgt aggacatccg
    13861 atttgtccca acgatcatat cgagattacc agtcaatcag tagatcaaat ggcagatcaa
    13921 gtaatggctt tgcctgagcg cacaaagatc caagtgttgg caccgatcgt ggtaaagaaa
    13981 aaaggacaac ataaaaaaat attcgaacga atccaaaaag agggatacgt ccgcgttcga
    14041 gtcgatggtg agatctatga tttaagtgaa gtgcctgagc tggagaaaaa caaaaaacat
    14101 gatatcgcga tcgtcatcga ccggatcatt gtgaaagaag ggattcgttc tcgtctattt
    14161 gattctttag aggcggctct gcgattggca gacggctatg ctctggttga cgtgatcggt
    14221 gaagaagaaa tgttgtttag cgaacactat gcctgtcctt attgcggatt tactgttggc
    14281 gaactagaac caagactgtt ctcattcaat gcgccttttg gagcttgtcc agactgtgat
    14341 ggtttaggtg tcaaattaga agtcgatcaa gatttagtga ttccagatag aacgaagaca
    14401 cttagtcaag gtgctatcgt tccatggaat ccaatcagct ctcagtatta tcctcaaatg
    14461 ttagcgcagg cttgtgaaag tttcggtatc gatttagata caccattcga agaattaccg
    14521 gaagagcatc aggatatcat cttgaatggg acagatgatc ttttccattt ccattatgaa
    14581 aatgattttg gtggcgttcg cgatgtagaa acgactttcg aaggggtatt gaagaatatc
    14641 gagcgtcgtt atcatgatac gaacagtgat ttcacgcgtg agcaaatgcg tctttatatg
    14701 acagaattaa cttgtcagac atgtaaaggt tatcgattga atccccaagc tctatctgtt
    14761 caaatcaatg gaacgaatat cgggcaaaca aatgagttgt ctattcaaaa agcagtagat
    14821 ttcttctcaa acttaacgtt atctgaacaa gagaaagtaa ttgccaaacc gattttaaaa
    14881 gaagtaaatg acagactttc attcttggaa aatgtgggac tagactatct aactttgagt
    14941 cgcgcttccg gaacattatc tgggggagaa gcgcagcgaa tcagattggc tacacaaatt
    15001 ggatcgaatc tttctggagt tttgtatatt ttagatgaac catcgatcgg attgcatcaa
    15061 cgcgacaacg atcgactgat tggctcatta aagaaaatgc gtgatcttgg caacaccctc
    15121 gttgtagtcg aacatgatga agatacgatg tgggcagcag attatctgat tgacgtcgga
    15181 cctggggccg gagaacaagg cggacaaatc gtggctgcag gcactcctga agaagtagct
    15241 aatgatgaaa attctttgac agggaaatat ctttcaggga aaaaatcaat ccctgtacca
    15301 aaagaacgcc gtaaaggtaa tgggaaagca atcaagatca ctggtgcttc agaaaacaat
    15361 ctgaaaaata ttagtgttga atttccatta ggagaatttg tagcagtcac aggtgtgtct
    15421 ggttctggaa agagtacatt ggtcaatagt atcttaaaga aatcgttagc gcaaaaatta
    15481 aataagaatt ctgctaagcc aggtaaattc aagacaattt ccggctacga aagtatcgaa
    15541 aagatcatcg atatcgatca aagcccaatc ggccggacgc cgagaagtaa tccagcgact
    15601 tatacaagtg tatttgatga tatccgtggg ttatttgctc aaacgaacga ggcaaaaatg
    15661 cggggttata agaaagggcg ttttagtttc aacgtaaaag gcggtcgttg tgaagcttgt
    15721 cgcggggatg gaattattaa gatcgaaatg cactttttgc ctgatgtcta tgttccttgt
    15781 gaagtatgtc atggcaaacg atataactct gaaacattag aagtgcatta caaaggaaaa
    15841 agcattgctg atattttgga aatgacagta gaagatgctg tagaattctt caagcacatt
    15901 ccaaagattc atcgcaaact gcaaacgatt gttgatgttg gcttaggtta tgtgacgatg
    15961 gggcaaccag caacgacatt gtccggtggt gaggcacaac ggatgaaact tgccagtgaa
    16021 ttgcacaaaa tctctaatgg aaagaatttc tatatactag atgaaccaac gacaggactt
    16081 catagcgatg acatcgcccg cttgttgcat gtattacaaa gattagtaga tgctggtaac
    16141 acagttttag tgattgaaca caatctagat gtaatcaaaa cagcagatta tatcattgat
    16201 ttaggaccag aaggtggaga aggtggagga acgatccttg cgactggaac accagaagaa
    16261 atcattaacg taaaagaaag ttatacaggt cactatttga aaaaaataat ggtataaaga
    16321 ggatagatag ctgagatagt taaaaaaaca taaaaaaatg aatttttgct tgttttatta
    16381 atatcagtgt tctagaatca ttacatggta gttctttcta cactgattgg tatagacaaa
    16441 aacttcctat aagttcaatc cgttcaaaaa agaattgagc aatcggagga gtttcctttg
    16501 attgcttttt tctttatggt atgatattcc ttgtgtagtt ggaaaagaaa aacaagagat
    16561 tatgagtaag tgagagataa tgatgaacag tattgaaaat gaagaattta tccctatatt
    16621 attaggaagc gacatgaacg tttatggtat ggcacgttct tttaatgaag catacggcaa
    16681 aatctgccaa gcatatgctt cggatcagtt agcccctacc cgctacagta aaattgtcaa
    16741 tgtagaagtt attccaggat ttgataaaga ccctgtgttt atcgagacga tgcttcgttt
    16801 ggcaaaagaa agatattctg ataagagtaa aaaatatctt ttgattgctt gtggtgatgg
    16861 atatgcagaa ttgatctccc aacataaaca agagctgtca gaatatttta tctgtccata
    16921 tattgattat tctttatttg aaagactgat caataaagta agcttttatg aagtttgtga
    16981 agaatatgat cttccgtatc ctaaaacatt gatcgtcaga gaagaaatgt tagtaaatgg
    17041 acatttggaa caggaattgc cttttgaatt cccagttgct ttgaaacctg caaacagtgt
    17101 ggagtatctt tctgtccaat ttgaaggaag aaaaaaagct tttattttag agactagaga
    17161 agagttcgat ttaattttag gccggatata cgaagccggt tataaaagtg aaatgattgt
    17221 acaagatttt atcccgggtg atgatagtaa tatgcgtgtg ctaaatgcgt atgtagatga
    17281 agatcatcaa gttcggatga tgtgtcttgg tcatccactg ttagaagatc ctacaccagc
    17341 atcgattggt aattacgttg tgatcatgcc agattataac gaaaaaatct atcaaaccat
    17401 taaggcattt ttggaaaaaa tcgaatatac aggctttgcc aatttcgata tgaagtatga
    17461 tcctagagat ggagaatata aattatttga aatcaatttg agacaaggtc gtagcagttt
    17521 ctttgtgaca ttgaacggct tgaatctggc tcgtttcgtc acagaagatc gagtgtttaa
    17581 caaacccttt gttgaaacga cctatggaac gaatcaatca gataaagcaa gattatggat
    17641 gggtgtgccc aaaaaaatct ttttagaata tgctcgggaa aatgaagaca aaaaactagc
    17701 tgagcaaatg atcaaagaaa atcgttacgg tactactgtg ttttatgaaa aagaccggtc
    17761 aatcaagcgc tggttgttga tgaaatatat gttccataac tatattccaa gattcaaaaa
    17821 gtattttcat gtgaaagaag gctagttcat ggagaatttt ttcagtattt taggcggaat
    17881 gggcacgatg gcgacagaaa gttttgttcg cttgatcaac caccgaacga aagctacaaa
    17941 agatcaagaa tatttgaatt atgtcttatt caatcatgca acagttcctg atcgtacagc
    18001 ttatatttta gatcgatcag aagaaaatcc aatgccgttt ctgctggatg atattgagaa
    18061 acaaaattta ttgcagccga acttcattgt gttgacttgt aacaccgcac actacttttt
    18121 tgaagaatta caagcggcaa cggatattcc tatccttcat atgccaagag aagcagcaaa
    18181 tgaacttgtc cgtcagcata cgacagggag agtagcgatt ctaggaacag aaggaagtat
    18241 gaaagctgga atctatgaac gcgaagtcaa aaatcttgga ttcgaaacag tgattcctga
    18301 tactgttctt caagaaaaaa tcaattatct gatctatcat gagatcaagg aatcggatta
    18361 tttgaatcaa gagctatatt acgaaatctt agaagaagcg gtagagcgcc taaactgtga
    18421 aaaagttatc ttaggttgca cagaattgtc attgatgcat gaatttgctg aagataacca
    18481 ttatccagtg atcgatgccc aatctatttt agcagatcgg acgattgaac gagcgctagc
    18541 tgaaagaagc aaggcattgg atacagcatc ggaaaagtaa aaaaattgga tttggggcaa
    18601 atatcctaaa agatatttgg tccaaatctt tttgtaaaag aaaaagaaaa ctggtaatga
    18661 tacgcccgga atgatataat tttactatgc acaatgtgag aggagttcgt aattatgaca
    18721 gataatttgc aattagtcat cataacaggt atgagcggtg cagggaaaac agtagctatc
    18781 caaagttttg aagatatggg ctatttttgt atcgataata tgccgcctcg attgattcca
    18841 aaattctggg aattaatcaa agaatcaggc aaagtcacaa aaatggcttt aggagtggat
    18901 ttacgttctc gttccttctt tgaagaaatc caaaacatgc tgatcgaaat cgaaaatacg
    18961 aacttcatcg acacttctat cttattttta gatgcttcag acgaagaact agtggctcgc
    19021 tacaaagaaa caagacgagc acatccaatg gcgatggacg gtttgatcac tgaagggata
    19081 cgtaaagaac gggcgatact agacgacttg aaagcccaag cttccgtgat cattgatacg
    19141 acgacattga caccacgaca attacgtgaa aaaatcaatg agtcattcaa atcaaaggga
    19201 gaccaaggat ttcggatcga atttgtatca tttggtttta aatacgggtt gccgatcgat
    19261 tcggatattg tgatggatgt gcgttttttg cccaaccctc attatatccc ggaactaaga
    19321 ccattgaccg gaaaagatca gccagtctat gattatgtga tgtcctttga tgaaacgcaa
    19381 tcattctacc ataaattttt ggatcttctg gaaactgtat tgcctggata cattaaagaa
    19441 ggaaaaagca gcttgacagt tgcaatcggc tgtacaggcg gtcaacaccg ttctgtggca
    19501 ttgactgaac gtttggcaaa tgctttaggg aaaaactata aagtcaatgt gacacatcgg
    19561 gacaaggata aaaggaaaga gacggtgaac cggtcgtgaa aatgaaaacc taccggataa
    19621 gaaaaccaaa gatcgtagtg gtcggcggtg gcactggtct gccagttatc ctgaaaagtt
    19681 tgcgaaatca aggagcagat atcacagcag tagtgactgt tgcagatgat gggggaagca
    19741 gcggtgcaat cagagaatcg atcgctatgg ctcctcctgg tgacttgaga aatgtcttag
    19801 tggcgttatc ggatatgcca caattctatg aagatatttt ccaataccgt ttcaaaaagg
    19861 aagatcagtt tttagccaat catacgatcg gtaatctgat cattgccgca gtttccgaga
    19921 tgcggggaag cacttatgag gcgattcaat tattagcaaa aatgatgcat gtagatggac
    19981 atgtttatcc ttcttcagaa actcctttga ccttgcatgc tgtctttaaa gacggaacga
    20041 ctgcagtagg agaatctaaa atcgcgatcg accgtaaaac cattgaccgt gtgtatgtct
    20101 gcaacacaaa tgataaaagt gaagccaaag cggctcgaaa agtcgtttca gcaattatgg
    20161 acgccgacat ggtcgttttg ggacccggaa gtttattcac cagtatcctt ccaaatttag
    20221 tgataccaga aatcggcgaa gcaatgctca agacaactgc tgaaaccgtc tatatctgta
    20281 atatcatgac ccaaaaaggg gaaacggaac atttcacaga tgccgatcat atacgtgttt
    20341 tacacaagca tttgaatcag ccttttatcg atacagtatt ggtcaatacg gaaaaagtgc
    20401 ctgaagatta tatggaccca gagatctatg atgagtatct agtacaagtc gcacacgact
    20461 tcaacggatt gagaaatgaa ggatgtcgag tgatttccac cgactttttg gaattgaaag
    20521 atggtggtgt cttccacgat ggagacaaag tagtagaaga attgttccgt ttagttttcg
    20581 gttcaaaata ttaagcaatt tattgttgga gaaaggagga gaataggatg tcatttgctg
    20641 cagatgtgaa gaaagaacta actggtttgg aagttcatcg agaacatgcg aaagcggaat
    20701 tagctgcttt gctgcgaatg aacggctctt taggtattgt gaaccagcag tttattttga
    20761 acgtccaaac agaaaatgct gcaattgcca gacgtatcta ttctcttttg aaagatcatt
    20821 atcaggtacg aagtgagcta ctcgttcgta aaaaaatgaa attaaaaaag aacaatgtct
    20881 atatcgtccg gttgaagcaa ggcacaaaag atgtgttgat ggatcttgat atcatggatg
    20941 gtatgatgtt caatggacac gtttcggacg agattatggg aaattcgcaa aaaatgcgtt
    21001 cttacttacg tggtgctttt atggcctcag gttcagtcaa taatccggaa acgagccgtt
    21061 atcatttgga aatattttct atttatgaac agcataacca agacatttgc gatatgttaa
    21121 attattacgg tctacatgct cgaacgcttg aacggcgcaa tggctatatt tcttatttaa
    21181 aaggagcaga aaaaattgct gatttcttaa cattgattgg tgcaaccaac tcaatgctcc
    21241 gatttgaaga tgttcgaatc gtacgggata tgagaaattc ggtcaatcgt ctagtcaatt
    21301 gtgagacagc aaatctgaac aagacgattg atgcggcttc taaacaaatc gaaaacatcg
    21361 aattgatcga agcaaaggtc ggtctacatg cccttcctga aaaacttcag gaaatcgcag
    21421 aacttcggct tcaacatcca gaggtcagct tgaaagaatt aggggaaatg attccctcag
    21481 gtgcgatatc aaaatcggga attaatcacc gaattcgcaa aataaatgaa tttgcggaaa
    21541 aactgcgtga agaagcagtt taatccaaag aaaaaactga tacatgaagc aggagatgcg
    21601 aatgtatcag ttttttttaa aagttatact tgcttttctt ccgtcaaact ataattccat
    21661 ctttactgga gtaacataat ttggaaaggc ttctattgtt tttaatacaa tcgtataaaa
    21721 agcggggaga caactgaatg aagattttag ttttgtacat aaatttttgc ttaatataga
    21781 agtcctgata tagataagat caagtattta tctataactg gcctgattaa ttaaaaaact
    21841 ggaaggaact gctcgatcat ccttgagaac tgctcttctt acttctggtc aagattctcg
    21901 tggttggtgg ataaggatgg ttgataagtt cattggatta gctagtcgaa aacaaccagg
    21961 tgtaattaat gtagaagttt taacaagggg atggagtgat ttattcagtg ataatcataa
    22021 gttcataaat agtaattgag atgtatggtt ggaatacgtt atacctgaac ttgaactacc
    22081 aagatttggt ttagaatttg agaatcgatg tttataaagg gagggcaaaa atgcgttttt
    22141 ttacaacttt tactatcatt atgattgcag tactttttat ttttctagat atagcaaagc
    22201 gaaatacagc atttttatta tatcgggttt tacttcgagc aggtctcata acatttatat
    22261 cgatcgttgg attttttcta ttcacagtta ttgtatttat ttggagaact ccagcaccac
    22321 cattgccaga gattacatat ggagaatttc cttttagact tgagtatgaa ttgaatgaag
    22381 aattacatgt gatagaagac acgttaatag ttgaatttga tggatttgga atgaacgaag
    22441 gcatagggag atatcgtaga tggacaagtc gtttagccag cggagaagat ttagtgttgc
    22501 tgttagaagt tagtgataat aagcaaatat tttattttcc aggacctgct aattattaca
    22561 tgggagatag gttgaatggt tataatcata cttttcctag tgcttctttc attgaaaggg
    22621 aaagaggcat tactagaagg gatattttac atgacaaaga attattagaa caatttggtc
    22681 cgcttgatca aaatacaata aatgaagaag aattgttaaa tcaatataat ataaggctag
    22741 tgaattggga aatatctgaa ccgattgtta acaattttgg tgattagaaa acaattacga
    22801 taaatagaaa caagccaaat catcagcaag taaaagctga tgatttggcc tttcttatac
    22861 gagtagtagg aattcatagc gaatattatt acgatcagta gatattttga tattaaagga
    22921 gtattacgaa ctagtatgtg agaatgtaga ggtgatttgc gaataatgag aaatttatca
    22981 aatagaaaca aaatattaat cattattgtt gttatagctg tgtttcatct gggaacgaat
    23041 gcggttttaa gtagaataat attgggtcct aagccaccaa gacctgcgat tacccgaggg
    23101 gagtttgatt ttagattaga atatgaagta gatggggaaa ggattgtgat agaagataca
    23161 attgttgcat tatttgatgg attttctgca gatgcaggta gcatggcatg gtatcgcacg
    23221 tggcgtttgc atttggcgag tgacaagagg gcacaacaat tattgttaca tgagttagat
    23281 gatggtcgaa aaatatatta tcattttgga ggagctggat tttatatggg gaagttagta
    23341 gcagtgtaac tgtaacaagg aaagatttgc caggtcttgg cattcgaaac tccatatttt
    23401 atatcgagcg tgggagagat acatctattg ggaacaggaa agggttaact cttgaagagt
    23461 tatataatga ctttggaatc cgtcttgtta gttgggaaca caatccacca atagaaaatc
    23521 gctttgaata atcgaaatct attaatccat ttcatcagcc agtgaatgtt gtaatggaac
    23581 aatttggttt agtgttcaaa accattgaaa tactaggctg tgatcagcaa aaatgtatct
    23641 gaataaaaaa gagagcaact attaaatgag aggagttagg agttgttagc tttatacgaa
    23701 tcaaagtggc aaatacagat gcgtatgcat ttgtgtttgc catcttgcgt ttggtagacg
    23761 agataggtat aagctaaaga aacaacttgt ttaaaataaa cgacctagct caacaaacga
    23821 ccatctaatc aactaacagc atacataaaa gcaactattt ttgaagtaga attgaaatcg
    23881 ggataactta aaaagtgatt gggatactag tgtgaatcac cgtacataaa tattactcgt
    23941 taagggaatt tgagaaattt tgtatgaaaa tttaagaatt agttagtatg cgcaagtcaa
    24001 aaatatgaat aaattatttg gtggagataa tttgctcagc ttgtcataag aaatgagata
    24061 atctggcaga tgcaagcttt tggacaccat ttattcggaa taaggaggct gggacagaag
    24121 cgtttaactc caagaaataa gaaggagttg cttctgcttc cgccgtttat aggtgtttag
    24181 agcgtgagac aaaagtgttc tttacttttg tcctacgctc cttatttttt tattttgttt
    24241 ggatcaattc ttttaattcc tctattgaaa tagattcatc aaagatttct ttacccaaga
    24301 tgacagtcgg aacaaatcga atatgtgctt gctcagcttc gttgatgaca gcttggctca
    24361 gctgttcgtc tttttgttct gtcagcttta gtttatctac tgcaaaatcc gctacttctt
    24421 ggagagaaag atgtttccac tcgtcttgag tatcgaatat ttgtttgatc tctttgatcg
    24481 ctttttgacc atcgctgatt gtgagatacc ggtgcatcac gtttcctcgc tgcaagcttt
    24541 ctttttcttt atcgaacaat ttgatcactc gctgcaattg cccgctttgg actgcttctt
    24601 ctaatagctc ataagattct tcaaaccatt gtcggcaata cggacaagat aggttgatga
    24661 attcgaccat ttttttagga gcattcgatg aaccataaag gattcctttt tgtgtatttg
    24721 ttttcgttgc atcaatcact gatatatcca tgttaaaaca cctctttcgc ttatagttta
    24781 tacatgtctg acaaaaaaag gcaacgattt agactgtttg tctaaaaatt agatgaccaa
    24841 ttcaaataga catatgtgaa atatttttat aaataaaaga aaaaatggaa tgttttacgt
    24901 tataatggaa cagataataa gagagaatat gaacaaggat gatgaatcat gcgaaaaatg
    24961 aaaacaatgg atggcaatac ggcagccgca tatatttctt atgcatttac cgagattgct
    25021 gcaatatatc caataacacc cagttcgacc atggctgaat tagtagatca atggtcagca
    25081 gcaggaaaag aaaatgtatt cggtcaacca gtaaaagtag tagaaatgca atccgaagca
    25141 ggagcagctg gtgtggtcca tggatcattg aaaactggtg cactgacaac tacatatacc
    25201 gcttctcaag ggctgctttt gatgataccg aatatgtaca agattgctgg ggaactactg
    25261 ccaagtgttt tccatgttgc tagtcgggca gtgacgacaa atgcgttgaa tattttcgga
    25321 gatcacggag acgtgatggc tgcaagacaa acaggatttg ctatgttagc tgaaagcagt
    25381 gtacaagaag tgatggattt agcacctgtt gcccatcttg cttctattga aggagaagta
    25441 ccttttttga atttctttga tggattccgt acgagtcacg agattcaaaa aatcgaagtt
    25501 ttggaatatg aagaattagc tcctctgatc aatcaggaga aattagcttc atttcgtaaa
    25561 cgagggatga atcccaatca tccgactgtt agtggtacga accagaatcc agacatccat
    25621 tttcaacaac gggaaacgat caacccctat tatgataaat tacctgaaat cgttcaaaaa
    25681 tatatgaaag agatcaaccg attacgagga acttcttatg acttggtgac ttattacgga
    25741 gcagacgatg cggaagaagt cattgtttct atgggatcag ttgcacagac gattgaacaa
    25801 acgattgatt acttgaatgc aaacggacgg aaaacaggtt ttttgaatat ccatctttat
    25861 cgtccttttc cagtggaaac attcgtgcaa aagttgcctc atacagtaaa aacaattgct
    25921 gttcttgatc gaagtaaaga accaggggca ggtggagaac ctctcttatt agacgttcaa
    25981 agtgcgttat atgattctga acttcgcccg gctgttattg gtggtcgcta cggattgggg
    26041 tctaaagatg tgactcccga ccaaatctca gcagtttttg atgaattgaa aaaagaccca
    26101 agcatacgca aaaaacggtt taccataggg atcgtggatg atgtgactta tcaatctttg
    26161 gaaccaaagg aaagtctgga tttgacagaa ccgcaaacat tccaggccaa gttttgggga
    26221 tttggatctg acggaacagt tggagcaaat aaatcagcta tcaagattat tggagatcac
    26281 accgataaat atgcgcaagg ctatttttat tatgattcga aaaaatctgg cggtctgact
    26341 gtctctcatc ttcgttttgg agatacgccg atacgttcag cgtatcttgt cgagcatgcg
    26401 gaccttgttg cctgtcacac accagcttac ctgcatagct atgacttggt caaaggattg
    26461 aagccaggtg gcattttttt attgaatact ctttggagcg acgaacaatt agaaacgcat
    26521 ctgcccttaa aattaaaacg ttacttggct gaaaataaca ttagattcta tacgatcaat
    26581 gccatgagat tggcccaaga agtcggtttg ggacgccgga tcaatacggc aatggaaacg
    26641 gcttttttta aattagctga catcattcct tttgatgagg tgcttccgct gctaaaagaa
    26701 gaagccctga aaagctatgg acataaatca atggctatcg tagaaaaaaa catacaagca
    26761 atcgatcgga cgaccgaact gcttcatcaa gtcgcgatcc cagaccattg gagaacgttg
    26821 gagatgcctg aaaaaatcaa taaaaggaac actggttata tacaagaaat acttgaacca
    26881 gtgaatgcac aggaaggaaa tcaactctct gttggtacac ttgtagcaaa tggaatgaca
    26941 actggcgaaa tgcctttagg aacggcttct atggaaaagc ggggcgtcgc tttagaagta
    27001 cctgagtgga ttagtgatcg ctgtacgatg tgtaatgaat gtgcctttgt ttgtcctcat
    27061 gctgccatcc gtccattttt agcagatgaa gaggaaatgg aagaagcgcc agaaggattt
    27121 atcgtgagag aaatgcgagg cgcagacggc gtaaaatacc gtatccaagt ttcagtggaa
    27181 gactgtacag gttgtggtct gtgtgtagag gcttgtcccg caaaagaaaa agctttagtg
    27241 atgaagcctt atgaagaaca gaaagaacaa gctgtgaatt gggcttttgc aatgacatta
    27301 agacaaaaag caaatccagt caaaggaaag aatactgtct tgtctacaca attcaatcaa
    27361 ccgcttcttg aattctcggg cgcttgctca ggctgtggcg aaacacctta tgtaaaatta
    27421 ttgacacaaa tgttcggtga tcgaatgatg atcgcgaatg cgacaggctg ttcttctatt
    27481 tggggcggtg cttctcctgc ttctccttat acgaccaatg aatgtggtca agggcctgca
    27541 tggtcaaatt ctttattgga agataatgct gaatttggtt atggtatgct tctagcggca
    27601 cagactcgtc gcgaggcttt ggcaagttca atcaaagaag tattgccaaa agtatcagaa
    27661 gaactacgag aattgatgga agactggttg gtacacatgt ttgagagcga aggaacgcag
    27721 caaagagcag caaaactgca agctgcgatc gaaagcgaac gatcaattgt ccctgaactg
    27781 gaggatatat acaagagaaa ggatctattc gtaaaaaaca gccaatggat gattggtgga
    27841 gacggatggg cttacgacat tggttttggc gggatcgatc atgtacttgc cagcggagca
    27901 gacgtgaata ttcttgtttt ggataacgaa gtctattcca atactggcgg tcaaacctca
    27961 aaagcaacac cagcttcggc aatcgctaaa ttttcggcta gtggaaagta tgcttcaaag
    28021 aaagatctag gaatgatggc gatgacatat ggaaatgttt atgtggcgca aattgcttca
    28081 ggtgccaatc aaatgcagac gataaaggct tttgaagaag cagaaaagta tcctggacct
    28141 tctttaatca ttgcctatac gccttgtatc acacatggtt tgcttggtgg aatgaaagaa
    28201 tcgcttaaag aagcaaaaga agctgtttct tctggctatt ggtcattata tcgatataac
    28261 cctgcaatgg tcgaaaaaaa caagaaccca atgactttgg actacaaaaa acctgatttc
    28321 tctgctatgc ctgatttcat gaggaaacaa gttcgctttt cctcgctaga gaacgcaaat
    28381 ccaaatcttg caaaagcttt atttgaaaag actatccaag acgctaaaac acgtttttac
    28441 aactatgctg cactgacggg agaattagaa aaaatcaaga aacgacttga accagaggaa
    28501 tctatcgatg tacccgttaa gactagaaaa gaaaaaacga cggaccttga agcagaagaa
    28561 agaagagctg ccagagcaga acgacgtaaa aaaatggaac aagctacaaa agaaaaaaag
    28621 gaaaaataac agaaaaaata taatgaataa ttctctgaag tttaaaaata tttattgcag
    28681 agaaaaataa aacgcatact tgcaattttc ctgaaaatta tgcgtttttc tcaagaaaaa
    28741 tacgagcaga tcagcctcta aaattaaaac atatgatata ctagagagga aatctcaaaa
    28801 gagaagagag gctttttatg tcatttcaaa tcggggaact ttttaatttg gactattggc
    28861 agcaaatgtt ttccggtgac ttgttctctc tgaatttttt ggtgaacata cttgatattc
    28921 tggttgtctg gtatcttgta tacaaactga tccaactgct gcgaggcaca aaagcgattc
    28981 agcttctgaa aggtgtagct atttttattg ttatccgaat cgtagcggaa atcattggtc
    29041 tgcacacctt gtcttggctg atgaaccaag tcattacgta cggggtgata gctgctatcg
    29101 tgatttttca gccggaaatc cggagaggac tggaacatct tggacgcagt tcgcttttca
    29161 aacaatcaaa aagcgaacag caggaagatg agaagatgat cttgtctttt gacaaagcca
    29221 tccaatatat gtccaagcga aaaatcggtg cgctaattac tatcgaacgg catactggat
    29281 tagatgagta tattgagaca ggaattgcat tagatgcaga tatcactgga gagctgctga
    29341 tcaatatctt tattcctaat acaccgctgc atgacggtgc agttattgta aaagaaggaa
    29401 agatcgcagt agcaagcgcc tatcttcctc tatcagaaag tatgttgatt ccaaaagaat
    29461 tcgggactcg acaccgggca gctgtcggta taagcgaagt cagcgacgcg attaccatcg
    29521 ttgtctctga agaaactggg gatgtcagca ttacattaga taatgaactg atggctggct
    29581 tatcccagca agaatatttg gcaattttgc gtagagaact gattgctgaa gagaaaacaa
    29641 aaaacaagaa aaacgtgatt caatctttcc tagacggtat gactaaggga ggggatcgca
    29701 aatgatctca aaagagaaac ggacaagcat tctttacagt tttattgcat tgctgttcag
    29761 tattgcgctg tacttcaatg caaatggcca gtccgttcca aatacgcttt ctggaaatga
    29821 agcgtataat caaacagtta ctggggtgcc aatcaagatt tcttatgatt caaagcgcta
    29881 ctatatccat ggatatgaaa atgcagtgac tgtcaaactt tccagtgcaa accgagttca
    29941 gttgaatgca gaggcaaacg aagatacacg gatgttccgc gtcacagcag atatcactaa
    30001 gctgggcgaa ggaacacatg aggttccgct aaaaattcaa aatttgagca gtgctgtcac
    30061 agcaaagatc gaaccatcga ctattacagt aacggttgag aaaaaggtaa taaaagattt
    30121 cgatgtggaa acgttgattc cttcaacgtt cacaccatct ggatatgaac ttgatgatac
    30181 ttccgttagt cctaaaaaag tctctatcac tacaggtgac aaaacattgg cagaaatcaa
    30241 acgagtagta gctgatgtgg acgcttcgat ggtcacagat gatgggatta atagtgaggt
    30301 cccaatacag gcgctgaatg cagcagggga agtattaagt atcgtctctg atccagtgca
    30361 agtaacagtg agagctgatt cgatcaagcc aagcaagtcg gtacgacttt atgggataca
    30421 gcaaggaaca ccagctgccg gggtagaaag ctatgatttc agtttttcaa aactggaagc
    30481 agaagtaagc ggctctagcg atttgcttgc atcgatcggt gacagtattg ctgtaccgat
    30541 caatgtctca ggtatcaatc atcgaacaac tcgtaaaatt gaaataccag tagaagaggg
    30601 gttaagtatc aagcccgaat cagttagtgt ggagattaca cccgtgttgc aaacgtcaag
    30661 cagtagctct gcttcgacga gcagtcggac acaagaaaat acaagtacga gtagaagtat
    30721 ttcatctgct gattcaggaa cggaaccatc gacaacaacc atttcttcca gttcggaatc
    30781 tgggacaagc agctcgacca caagtacaga agaaacatca gaaacaagcg aatcaacaca
    30841 aatgagcaga acaaattcct cagaaaatta agttaaagga gtaattttac aactatgggt
    30901 aaatattttg gaacagacgg cgtacgcggt gaagccaaca aggaacttac gccagaactt
    30961 gcatttaagc tgggacggtg cggaggttat gtcctaagcc agcatgaaga agaaggaaaa
    31021 cgcccacgcg tgttagtagg gcgggataca agaatctcag gtcaaatgtt agaagaagcg
    31081 ctgatttcag gacttttatc ggtgggtatc gaagtgttcc agctaggtgt gatctctaca
    31141 ccaggtgttg cttatctgac acgtttgcaa aaagcaagcg caggggtcat gatttctgct
    31201 tctcataatc cagcacaaga taatggaatc aaatttttcg gtgcagatgg ttttaaacta
    31261 gtagatgacc aagagctaga aattgaagca ttattagatg ctgaagtgga tgaattgcct
    31321 cgtccatcag ctgaaggatt gggaactgta gaagaatttc cagaaggctt attgaaatat
    31381 tctcaattcc ttgtacaaac gatcaacggt gacctttccg gtttgaccat ttgtgtcgat
    31441 gcagcaaacg gagcaacttc aacaagtgtt aatcgtttgt ttgccgatct tgaaacagat
    31501 ttttatacca tgggcacaaa tccagacggg ttaaatatca atgatggcgt aggctctacc
    31561 catccagaac gtttagctga gatggtcgtt gaaaaaggag cagatgctgg actagctttc
    31621 gatggcgatg gcgatcgtat tattgcggtg gatgaacttg gaaacattgt cgacggtgat
    31681 aaaatcatgt ttatctgtgc taaatacttg gcagaaaaaa atcgattgaa aaaagatacg
    31741 atcgttacga ctgtcatgag taacttagga ttccacaaag cggtggaagg aatcggtttg
    31801 aaagatgtcg ttacacaagt tggtgatcgt tacgttgtag aagaaatgcg caaaaacgat
    31861 tataactttg gcggcgagca gtctgggcac atgattttct tagattttaa cacaactgga
    31921 gatgggatgc tttcaggaat tcagttactg aatatcatga aacagacagg taagaaatta
    31981 tcagagcttg cagcagaggt aacaatctat ccgcaaaaat tagtgaatat ccgagtaagt
    32041 gataaaaacg gtgcgatgga tgttccggca atcaaaaaag tgattgaaga ggcagaagct
    32101 gaaatgggat cagaaggaag aatcctagtt cgtccttccg gtacagaacc attacttcga
    32161 gtgatggcag aagcgcctac tgatgagaaa gttaattact atgtcgataa aatcgcggca
    32221 gtagtcaaag aagaaatcgg actggattaa gagatatttt cataaaaaat aaaagaacaa
    32281 ggaccagtgt cagatgtctg acactggtcc ttgttctttt attattctac tgtcactgat
    32341 ttcgctaaat tacgtggttt gtcaacgtcg aatccccgta atagcgttgc gtaatatgca
    32401 agtaattgag tcggtacgac tgtggttaga gtagttagat aagggtggac agttggtagc
    32461 accagctggt cactttcctt ttctaatccc tctgagacaa taactagtgt tttagcgcca
    32521 cggctttcga cttctttcag gtttcctcga gtatgcgcac cagtcacttc ttcactgatg
    32581 attccaatca ctggtgttcc ttcttcgatc aaagcaatcg ttccatgttt caattcgcca
    32641 gcggcaaaac cttctgcctg gatataactg atttctttca atttcaaagc agcttcaaga
    32701 gaaacgttgt agtcagcgcc acgtccaatg taaaaggcat ttcttgtatc gctgagatat
    32761 acttctgcca actcttctaa ataatctttt tgggcgatga ccgattccat cgcattagca
    32821 actaaactca attcatgagt gatatcgaat acaagagaag tttcaaattc ttttttatca
    32881 ccgatagctt tggctaataa agttagtaca gctatttgtg cagtataagc tttcgttgat
    32941 gctactgcga tttcgggtcc agcatgcaat agcagtgtaa aggatgcttc ccgcgaaaga
    33001 gtagaaccag caacatttgt aatcgtaagt gaagggtagc cgagacggtt gatttttacc
    33061 agaacttggc ggctatctgc tgtttcgcca ctttgactca agaaaataaa gaatggtttt
    33121 tttgaaagaa gaggtgtgtt atagccaaat tcactggcta aatgaacctc aacagggatc
    33181 tgtgtcaatt tttccagagt ctgtttacct gctaaccccg catggtaact agttccgcaa
    33241 gcaacgatat agattcgatc gctatcagac aatgtatcga ccagcttttg atcaagagag
    33301 acacgattgt cttctgttac gtatttttga gcgatacgac gcatgataat aggctgttcg
    33361 tcgatttctt tcagcatata aaaaggatag gtacctttct cgatatctga agaatctact
    33421 tgtgcttcga atgaagaacg ttctacgatg tcacctgcaa aattttgaat aataactttt
    33481 tctgatgtga cggtaaccat ctcttcatct tcaatctcaa cgaattcctt cgtatgagca
    33541 agcatagcca ttgcatcgct ggcaatcaca ttgaatccat cgccgagtcc gattaatagt
    33601 ggacttttat ttttagcgac gtaaataaca tcaggtgctg tccgatcaat caaagcgaaa
    33661 gcgtaagaac ctttgatgac ccgcaaagct tttaaaaacg cctcttgcgt tgtagtatcc
    33721 ttcgcgaaag tttcaattag atgagcgata atctcagtat cggtctcacc aataaagtgg
    33781 tcatttgcta aataagcaat tttaagttct tcaaagtttt cgattacgcc attgtgaacg
    33841 agcacgaagc gtccactttg ggaagtatgt ggatgggcgt tttcttcaga aggttgcccg
    33901 tgagttgccc agcgtgtatg accaatacct atatttcctg tagtatcaat atttattttg
    33961 tttttcaaat tttgaattct gccttgtgct ttgaccagat aatcagttgt gccatctgag
    34021 acaaagatac cagctgaatc ataaccacgg tattctaatt tttctagacc gtcaatcaat
    34081 cctggtgtta cattttttaa tccaatcatt ccaacaattc cgcacataaa aaacatccta
    34141 ctttctataa tgaattcgta tgaatgcttc cagctgcagg aaaatccttt tgttttatct
    34201 ttgcagatat tttttgtgga ttttctgtag agtgcagtcc ctagggccat ccgccgaatc
    34261 tttcgataaa ccctctcctc gtcatctgtt tacacagacc tggcgctatc ctaaaaggta
    34321 taactatcct cctcttttgt tttttatctg gaaacattta aaatcataac cttttgcgaa
    34381 tgttcttgtc aacaaaaaaa gaataaaaaa taaattggta tatatcaaat gctttgtcaa
    34441 atgctctttt tatgtcaaaa aataatgggt ttttatctat catctttttc aggagttcgg
    34501 tacaatagaa agtaatcgga tagaggagga gtaaaaatgg attcaaaaat tgagatcatg
    34561 acactgggaa tgttaaaaaa acaattgtcc aaattcgaag catcagctgg tgtatcagat
    34621 gatacgaaaa tatttttaga tactggctgg gatagcatcc aagaaatcgc acctgatgcc
    34681 ttggaagttg ctgaagctcg tgagtttacg gtagaagatg aattgacaaa agagtcgttt
    34741 tccgggtatg cgcgggaaga aaaagcagag cggttcgaca cttctgagca gtcagaaact
    34801 gtgatcgtca taaaaaactt gtattagaaa ggagaaagaa tgaataagag acgttgggca
    34861 gcagttctga ttgctgcagg attatttgtg gtttcgttag tttcagctag tttgactaag
    34921 cctcaggaaa acgatcaaat tgagggattg aacagttggt tatatgggga tgaagaacta
    34981 gcttcaaatg tgttggaaga gggagacagt acgcaaaaaa tcgtaaaatt atcagtagat
    35041 ggaacgattg ctgcgggagg ggcttcaggt ttatttgctt ctgaaggtta caatcataaa
    35101 aattttatgg aacaactgaa ggcgattgaa gaagatccaa cagtcaaagg tatcttttta
    35161 gaagtaaatt ctcctggtgg cggtgtctac gaaagtgcgg aaatcgctaa gaaattggat
    35221 acgattcgta aagaacatga tatcccaatg tatgtcagca tgaaaaacat ggcagctagc
    35281 ggtggctact atatttctgc tcaagcagat aaaatctttg caacagaaga aacagtgacc
    35341 ggctcaattg gtgtgatcat gtcaggattg aattattcag gacttctaga aaaactgggt
    35401 gtggaagata caacagttaa aagcggagcg ttgaaagata tgggttcagg aacgcgacca
    35461 gagacaaaag aagaagaagc ggtgcttcaa gcatatatcg acagcgcata tagtcgtttt
    35521 gtagcaatcg tcagcgaggg acggaataaa tctgaagaag aagtcaaaaa aattgctgat
    35581 ggaaggattt atgatggtgt ccaagcaaaa gaagtaggtt tagtcgatga attgggattt
    35641 cctgaagatg ctttaaaagc tatgcgaaag gaacaacagc ttgaagatgc ggaactagta
    35701 gaatattctt ccacgccatc tgggtttgct agtacatggt taggaagtaa actagcagaa
    35761 ttccagggat tgaagccttc tgagactagt cagatcttat ccgttcttga gaatctaggg
    35821 acagcggaat caccaaaagc aatgtattat tacggaggtg agtaagcatg tcagagcttt
    35881 caaatgaaaa taaggaacaa gaaaaagata agcaacagca gcctgctgta ggacctgact
    35941 ccaacttttc ccaaaaggta ctgagctctt ttgaaagaga gcctttgacg cccgaagaaa
    36001 tcaaaaagaa acaacgtaaa tggaaaaagt atacggacac aagaaatgaa ccgccaaaag
    36061 caaatgattt tcctaagtat ttctatgcag gtttttttat ccgaatgttt gctttcttgg
    36121 tcgatctttt gtgtatcgga gctattacgc gtttaacact tggtattgcc aaaaatctag
    36181 gttggcttaa tttttcagat tcttatctga gtatctacgg attcacagcc ttacttattt
    36241 atctagcgta ttttatttta ttgacgaaat tcaatcatgg tcaaacgatt gggaaaatga
    36301 tctttggtat tcgagtcatc agctttgatg aagaagaact tagctggtca accgttttag
    36361 ttagagaagg tgcttgccgg ttcattctaa aatttccttt gctgatgtta gggtatcttc
    36421 cagcaatctt taatcagaaa aagcaacatc taggcgattt tttcactaat acaagtgttg
    36481 ttacgatcaa tctgatcaaa gcattcaacc aggaagtcaa cagctgatga gcaataaaaa
    36541 gcagtaacag ttgaaagcac cttctaaaag tcagatctaa aatctagctt ttagagggtg
    36601 cttcagttat ttttaattgt catgcttttt ttgttttgct actattgaac atgagctgaa
    36661 catgagcaag aggaggacac tgcgtgaagc cgattgattt tccagaaaaa tatgagaatt
    36721 taatgcgagt agcacaacaa gcactagcaa accagcaata tcagcaggca aaagaattat
    36781 ttcaacgagc ttacgagttg aaagaaagct ttgaggctaa tagcctgctt gttttttgtc
    36841 tttacgagtt ggacgagaaa aaagaagcat tgaagcaagc actcttgcat gaaaaacagt
    36901 atcttcaaaa tgaggagttt gctgaatttt attttgactt gttaatcagt acacaagatt
    36961 ttttatatgc tagaaaattg atagcaagta cagattttta tgaagctttc gagcagcggg
    37021 tcattgaaaa aatccaattt gcagaagaat tatccggaca aatggagagg cagaagatca
    37081 aggcactcca caataaatcg gaggaacttc cttcgttgga acctgcacgt cagcttgcgt
    37141 ctattgatga gatcgaacag cttccttatc atgagtttat ccaaacagcg agtaaactta
    37201 ttgtatcgcc agaggtccat attttagcaa gggcaaaatt actggagaca cttaggcaat
    37261 tgaacgaatg ccatcctgtt ttctatttga cgattgaaga aaagttagtc aaagtcatac
    37321 caaaagatct gccaaagccg cagcagcagg caagttacca gcaattatgt gtattttcag
    37381 atcgttacgg taatgaagat gcgctgctta gttccgtgtt aaatgaagaa ttcacgttgc
    37441 aatctgcaat tgtttatcct gtctatgata catacatcga agacccgaag cgctggtttc
    37501 aactgactgt agaggcgtgt acaggaaaaa cgatgtatga aggtacagaa gatgaaaaac
    37561 aagatttttt aaaaaaaaga gaaaaaattt tgcagcaaat gatcttcttc cattagattt
    37621 tatcagtgaa aaagataaaa atgttatgaa aaacaaggat tctatccatt caatatgttt
    37681 acaatttgaa agggttagtg tactatttaa aggtatgcat gaaacgtttt tgatgtataa
    37741 aatgaatttc taaactcgga gggaattaaa tgactgcaac atttgaaaaa aaaggcacga
    37801 atgatggtgt tttaaaattt acgattgcgc aagacgaaat ccaaaaagga ttaacaactg
    37861 cgtttaataa agtgaaaaaa agcttgaacg tacctggatt ccgtaaaggg aaagtttctc
    37921 gtcaagtatt caaccgtatg tacggtgaag aagctttata tgaagatgca ttgaatgctg
    37981 tattaccaga agcttatgaa gctgctgtca aagaagctgg catcgatcct gtttctcaac
    38041 caaaaatcga tgttgaaagc atggaaaaag gtcaagactg ggtaatcact gctgaagtaa
    38101 cagtgaaacc agaagtaaaa ttaggacaat ataaagatct agttgttgaa aaacaagacc
    38161 gtgaagtaac agatgctgac gtggatgcac gtatcaaacg tgaacaagaa gcacaagctg
    38221 aattagttat caaagaagat gctgcagctg aaaatggcga tacagtagta atcgacttcg
    38281 aaggctttgt agatggcgaa gcgtttgatg gcggaaaagg cgaaaattac tcacttgaat
    38341 taggttcagg ctcattcatt ccaggttttg aagatcaatt agttggtcat aaatcaggtg
    38401 aaagtctaga tgtaaatgta actttcccag aagattatca agcagaagat cttgctggaa
    38461 aagaagcagt cttcaaagtg acaatccatg aagtaaaagc aaaagaagtg cctgaattag
    38521 atgacgaatt cgcaaaagat gttgatgacg aagtatcaac attagacgaa ttgaaagaaa
    38581 aatatcgcaa agaattagta gaaacaaaag aaaaagcagc ggacgatgca aaagatgaag
    38641 cagcgatccg tatggctgtt gaaaacgcag aaatcgttga attgccacac gtgatggttc
    38701 atgatgaagt acatcgttca atggacgaat tcttgaacaa catgcaacgt caaggtatct
    38761 ctcctgaaat gtactatcaa ttaactggtt ctacagaaga agacttgcat aaacaattcg
    38821 aaggtgaagc agaaatgcgc acaagaacaa atcttgtgat cgaagcaatt gcagctgctg
    38881 aaaatcttca agcaacagaa gaagaaatca atgcagaaat ccaagaatta tcagaaacat
    38941 acaacatgcc aatcgaacaa atcaaacgcg tgttgacaga agatatgctg gaacatgatg
    39001 tgacaatgaa aaaagcaatc gaattgatca caggtacagc gacagaagcg aaagaataga
    39061 aatcaagtaa taaatcattt ctgaagaagc tacttctaga aaattgttta ttcgaaataa
    39121 gggactgtga cacgattttg tcacagttcc ttattttttg ttaagagtaa aaaatgcaaa
    39181 ggtataggtc agttgatcct ttgttaaaca ctttttcttt ttactaaact atgttatgat
    39241 agtaaaagac tgtaaagtct ttgatagttt cttgcatact ttttgcaagt taaggggtga
    39301 agaccatgta tgacaatcca agtagcaatg agaccgtacg ctgttctttt tgcgggaaaa
    39361 cccaagaaga agtgaaaaag atcgtagctg gtccaggggt ctacatctgt aatgaatgta
    39421 tcgacctatg taaagaaatc attgatgaag aattttatga agaagctgta cgcgaattca
    39481 ctgaagtccc taaaccacta gaaattcttg aagtattgaa taactacgtg attggacaag
    39541 atcgcgcaaa acgctcgtta gctgttgccg tatacaatca ctataaacgt gtcaatcagc
    39601 aagcacaaga aaacacagat gatgttgaac tgcaaaaaag caatatctgc ttgatcggac
    39661 ctacagggtc tggtaaaaca tttttagcac aaacattagc aaaaacattg aatgtacctt
    39721 ttgcaatcgc agatgcgaca agtttgacag aagctggtta tgtgggggaa gatgtagaaa
    39781 acattttgct taaattgctt caatctgctg attacaatgt ggaacgtgca gaaaaaggga
    39841 tcatctatat tgatgagatc gataaaattg cgcgtaaaag cgaaaatgta tctattactc
    39901 gtgacgtttc cggcgaaggt gtccaacaag ccttgctgaa aattctagaa ggaactgttg
    39961 caagcgtgcc acctcaggga ggacgtaaac atccacatca agaatttatt cagattgata
    40021 cgacgaacat attgttcatc gtaggtggtg catttgatgg catcgaaacg attgtgaaga
    40081 atcgtatggg cgaaaagacg attggatttg gtacacaaaa ccaaaaactt tctgaagacg
    40141 aaagtgtcat gcagcatatc attcctgaag atttactaaa attcggattg atccctgagt
    40201 ttatcggacg attacctgtt atggcagctt tagagaaatt gacaacagat gatctggtac
    40261 ggatcttgac tgagccgaaa aatgcgctag tcaaacaata tcaaaaactt ctttctctag
    40321 ataataccga acttgaattc gaacctgaag cgttgagagc cattgcgaaa aaagcaatcg
    40381 aacgcaatac cggtgctcga ggacttcgtt ccatcattga agaaatcatg atggatgtga
    40441 tgtttgatat cccttcaaat gaaaacatcg aaaaagtgat tatcacaaaa gaagcagctg
    40501 aattgtctgg tgagccaaca gtaatctata aagattcaga gaaaaaagcg ggttgatgaa
    40561 aagaggctgc cgtaaaagtc attttggctt ttgcgacagc cgcttgcttt attcatcacc
    40621 ttgttaagga gagagaccat gaaagtccat caagctgaaa ttatcataag tgcagtggca
    40681 ccaaaacaat atccagatac acaattgcca gaaatagctc tggcaggacg atcaaatgta
    40741 gggaaatctt cttttatcaa cacattgatt gatcgaaaga atttagcccg tacttccggt
    40801 aagccaggaa agacccagac attgaatttt tatttgatcg agaacgcatt acattttgtc
    40861 gatgttccgg gatatgggta tgcaaaagta tccaaaactg agcgagcaaa atggggaaag
    40921 atgatcgaga catatttaac gactagagac cagctacgtg cggtcgtttc tttagtagat
    40981 ttgcgtcata agccttctca agaagatatc cagatgtatc aatttttgaa gtattatgat
    41041 atccctgtaa ttgttgttgc cactaaggca gataagatcc ctcgtggtaa atggaacaag
    41101 catgagagcg aaatcaaaaa agcgttggat tttgatcgca atgacacgtt tattctcttt
    41161 tcttccgtta caaaacaggg aaaagaagaa gcgtgggcag caatcgaaca agcaattgaa
    41221 tcataaaaag aataaaaaag acttaccagt caaactcttt cataaaa
//
LOCUS       DACCXO010000015        40654 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.015, whole genome shotgun sequence.
ACCESSION   DACCXO010000015 DACCXO010000000
VERSION     DACCXO010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.015"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            283..1983
                     /locus_tag="IVY70_000730"
     CDS             283..1983
                     /locus_tag="IVY70_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP7957882.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            1953..2171
                     /locus_tag="IVY70_000731"
     CDS             1953..2171
                     /locus_tag="IVY70_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957883.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            2191..3081
                     /locus_tag="IVY70_000732"
     CDS             2191..3081
                     /locus_tag="IVY70_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP7957884.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            3099..3383
                     /locus_tag="IVY70_000733"
     CDS             3099..3383
                     /locus_tag="IVY70_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957885.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            3870..4484
                     /gene="gmk"
                     /locus_tag="IVY70_000734"
     CDS             3870..4484
                     /gene="gmk"
                     /locus_tag="IVY70_000734"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP7957886.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            4489..4794
                     /locus_tag="IVY70_000735"
     CDS             4489..4794
                     /locus_tag="IVY70_000735"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP7957887.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            5047..7455
                     /gene="priA"
                     /locus_tag="IVY70_000736"
     CDS             5047..7455
                     /gene="priA"
                     /locus_tag="IVY70_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP7957888.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            7470..7961
                     /gene="def"
                     /locus_tag="IVY70_000737"
     CDS             7470..7961
                     /gene="def"
                     /locus_tag="IVY70_000737"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP7957889.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            7954..8892
                     /locus_tag="IVY70_000738"
     CDS             7954..8892
                     /locus_tag="IVY70_000738"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP7957890.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            8892..10250
                     /gene="rsmB"
                     /locus_tag="IVY70_000739"
     CDS             8892..10250
                     /gene="rsmB"
                     /locus_tag="IVY70_000739"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP7957891.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            10263..11003
                     /locus_tag="IVY70_000740"
     CDS             10263..11003
                     /locus_tag="IVY70_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP7957892.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            11000..13069
                     /gene="pknB"
                     /locus_tag="IVY70_000741"
     CDS             11000..13069
                     /gene="pknB"
                     /locus_tag="IVY70_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP7957893.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            13234..14133
                     /gene="rsgA"
                     /locus_tag="IVY70_000742"
     CDS             13234..14133
                     /gene="rsgA"
                     /locus_tag="IVY70_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP7957894.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            14146..14796
                     /locus_tag="IVY70_000743"
     CDS             14146..14796
                     /locus_tag="IVY70_000743"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP7957895.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            14797..15435
                     /locus_tag="IVY70_000744"
     CDS             14797..15435
                     /locus_tag="IVY70_000744"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP7957896.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            15590..16444
                     /gene="mreC"
                     /locus_tag="IVY70_000745"
     CDS             15590..16444
                     /gene="mreC"
                     /locus_tag="IVY70_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP7957897.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            16448..16957
                     /gene="mreD"
                     /locus_tag="IVY70_000746"
     CDS             16448..16957
                     /gene="mreD"
                     /locus_tag="IVY70_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP7957898.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            17212..18822
                     /locus_tag="IVY70_000747"
     CDS             17212..18822
                     /locus_tag="IVY70_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957899.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTG
                     SSTPAPTPTPTPEQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCS
                     GFTRYVYMQVTGRDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGG
                     QYIHAPQPGESVKVGSVQWFAPDFAVSM"
     gene            complement(18890..20689)
                     /locus_tag="IVY70_000748"
     CDS             complement(18890..20689)
                     /locus_tag="IVY70_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP7957900.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            20812..21318
                     /locus_tag="IVY70_000749"
     CDS             20812..21318
                     /locus_tag="IVY70_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP7957901.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            21397..22119
                     /locus_tag="IVY70_000750"
     CDS             21397..22119
                     /locus_tag="IVY70_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7957902.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(22197..23597)
                     /locus_tag="IVY70_000751"
     CDS             complement(22197..23597)
                     /locus_tag="IVY70_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP7957903.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(23767..24741)
                     /locus_tag="IVY70_000752"
     CDS             complement(23767..24741)
                     /locus_tag="IVY70_000752"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP7957904.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            25094..25654
                     /locus_tag="IVY70_000753"
     CDS             25094..25654
                     /locus_tag="IVY70_000753"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP7957905.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            25671..29192
                     /gene="mfd"
                     /locus_tag="IVY70_000754"
     CDS             25671..29192
                     /gene="mfd"
                     /locus_tag="IVY70_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP7957906.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            29207..30802
                     /locus_tag="IVY70_000755"
     CDS             29207..30802
                     /locus_tag="IVY70_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7957907.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            30813..31082
                     /locus_tag="IVY70_000756"
     CDS             30813..31082
                     /locus_tag="IVY70_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP7957908.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            31149..31574
                     /locus_tag="IVY70_000757"
     CDS             31149..31574
                     /locus_tag="IVY70_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP7957909.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            31620..32090
                     /locus_tag="IVY70_000758"
     CDS             31620..32090
                     /locus_tag="IVY70_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP7957910.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            32210..33655
                     /gene="tilS"
                     /locus_tag="IVY70_000759"
     CDS             32210..33655
                     /gene="tilS"
                     /locus_tag="IVY70_000759"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP7957911.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            33655..34200
                     /gene="hpt"
                     /locus_tag="IVY70_000760"
     CDS             33655..34200
                     /gene="hpt"
                     /locus_tag="IVY70_000760"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP7957912.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            34290..36401
                     /gene="hflB"
                     /locus_tag="IVY70_000761"
     CDS             34290..36401
                     /gene="hflB"
                     /locus_tag="IVY70_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP7957913.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            36618..37505
                     /gene="hslO"
                     /locus_tag="IVY70_000762"
     CDS             36618..37505
                     /gene="hslO"
                     /locus_tag="IVY70_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP7957914.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            37506..38510
                     /gene="dusB"
                     /locus_tag="IVY70_000763"
     CDS             37506..38510
                     /gene="dusB"
                     /locus_tag="IVY70_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP7957915.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            38621..40117
                     /gene="lysS"
                     /locus_tag="IVY70_000764"
     CDS             38621..40117
                     /gene="lysS"
                     /locus_tag="IVY70_000764"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP7957916.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
BASE COUNT        13473 a         7364 c         8806 g        11011 t
ORIGIN      
        1 aaactgactc agtcatcatg ttcctcctct tatttcctct taatctcttt tattttacaa
       61 catttgcatt aaaaaagctt gtttctttta ttttaaaatt ccattacaaa atcacgtttt
      121 cattgaaaac actctcattt tcaattaatt tttgtgattt ttgtattttt ttatttaaag
      181 gaggttttat gaattgtttt cttatggaat ctacaaagtc gaatacgaga ctcatgacct
      241 ttttatgacg tactggtata cttgaagaaa aaggagagcg cgatggagat aaaaaaagtt
      301 tacttttcac agaaaatgtc gctgtattcg cctatccagc aagcaaaaca accccttcca
      361 ttttttcaaa gtcatcaaga aagtagagac gctttttggc gttctgcaaa aaagcaatgt
      421 gcttttaaag cagtatcgaa aaaatatacc gtctatttta ctattgctaa aagtagagga
      481 gatgttattt ttaataatct gttgattgaa ggtgaaactt ttgaatggaa aaaatttttt
      541 cggtatatgg aagcttgcgc tcgttttttt attaaagaaa actgttgttt tttatttcaa
      601 tctcctctat cagaagaatg gttgcgtatt tttcataaaa acggctatca aggaaccaca
      661 caattaaata aaaagctggt ctatcataca gcacttgttt taggtggagg aggagcgcac
      721 ggtgcttatc agattggtgt gtggcaggcg ctgaaggaac atgatatcaa ctttgagatt
      781 atcacaggta cctcggtagg tgcgttgaac ggcgcattga ttttacaagg agatatgaaa
      841 aaagctgtga atttatggaa aaagctgtcg acaagacaag tgcttgcgtt accagaaatg
      901 gctgcagttg aagaccttcg tgaacgcttc tatcgagaaa gacgccaaat gacgaaaaca
      961 gcactaattg aaggcggggt ttcttctgct ccattggaaa agatggtcaa agacaatttg
     1021 gatttgtcct tactcaaaca taatcccaaa atacgtttat atacggtgtc tactaaactg
     1081 ccagaactag cggaagtagt gactgatatc caacagacaa aaacagaaga aataccagat
     1141 tggatactcg cctctgcttc attttatccc gctatggctt atcgaaagat cggaaaaaat
     1201 aaatatgcag acggtgggta tcgcaacaaa atacctattg atattgcgat taacaaagga
     1261 gcaacagagg catttgtcgt agatgtgcaa gggccaggcc cagcgaaaaa aattcgtatg
     1321 ccagacattt ttatccattg gaaatgtcaa acattgtgga cactaggcag ctttctgctt
     1381 tttgatagcc aaaggaacca attaaattta caattaggct atctggaaat gaaaaaacga
     1441 ttaggctatt actatgggaa ctggtataca tttcattctg ttaaacctgc aagaacatac
     1501 tggcgaggat ttttgtctta cttaacaaaa gaaattcagt tagaattatc ttttttcaaa
     1561 agcataaaat tttggcaaaa actgcgtaat ttatataaga acagggtcgt accagagaca
     1621 tgcggattgg cgatgctaga acttttagca aaaaaacatt ttttactgcc caatgaaatc
     1681 tatcaagtag acatgatgat tcaaatgatc tgccaacaag atataaaaag tatgccggat
     1741 gatttattgg cacaaattgg tcaattaagt actgaagaat ggcgaaggta tcgaaaatac
     1801 cagcaaaaaa tacaaaatga gcgcacgaaa actgtttatt ttgattatct gctacaagga
     1861 aaacgcaagg atcgtttgca gcatgaattg cttaagcaac cagttgacac attactgatc
     1921 ctatatttat attatctcaa ggaggaacaa ccatggcaca agaattttca tatgaaatcg
     1981 tagaagaaat cgctatattg tcagaaaaca acaaggggtg gcgtaaagaa ctgaacctag
     2041 tcagctggaa tggtcgcccg ccaaaattcg atcttcgaga ttgggcgcca gaccatgaaa
     2101 aaatggggaa aggcctgaca ttgacgaacg aagagtttga acaactgcaa aaagcaatag
     2161 aaaatatgta atgaggaagg accctgtcga atgaaaagta tgactggttt tggcaaagcc
     2221 atccgcgaaa caaaagcgta tcagttagat attgaaataa aaagtgtcaa ccagcgtttt
     2281 ctagatgttc aaatacgaag tcctaaaatc ctgaactttg ttgaaaacga ttttcgtcag
     2341 cttattaaac agcagttaag ccgcggtcga atcgaagtat ttgtgaatct agcttatatt
     2401 ggcaaaaacg aaaaacagat cgtcatcaac tgggaattga ttgattcact tatcactcaa
     2461 ttgacagacg gaattcaaca acgatacggt agtcaaaatc aagtgtcaat tagtcggatg
     2521 gcggaacgct tcgtgctgga cgaaaacttc gtaacggtag aagaaaaacc ggaagagagc
     2581 atggaagaat tggcacagtt agctctagat acattgaaaa gcgcacttga ttctattgag
     2641 gcgagtcgag aaaaagaagg tcaagcttta gctttagtgt tgaaaaagaa tactgaagaa
     2701 ttcaaagaag tactaaaaaa actcgatagt tttgtagaaa tctatgaaaa ggaatttcaa
     2761 ataaggtatc aaaaaaagct ggaggaattt cttggagcga aagtcgatga gcaacggttg
     2821 ttgacggagc tagccatctt gttggaacgc ggagatattc acgaggaact ggatcgtctg
     2881 gcgatccatg tacaaaaact agatgaactt atcgaagcac aatgtcctgt aggtcgggaa
     2941 ttagatttcc tgatccaaga aatgaatcgg gaaatcaaca cgatcggttc caaatcaaat
     3001 gcaatcgaaa ttaaaaatca agtcgtccag tctaagacga tcttagaaaa aatcagagaa
     3061 caagtacaaa atattgaatg acgaaagagg tagagataat gaaatattat gacgtaacgt
     3121 tccacgaatt aagtggaaaa actgtggtta aacgggatat cccttctgaa aaaaacggct
     3181 ttgacgtatg gaaagatgcc tgtgcagact ataatgagaa cgaactgttt attttgatca
     3241 acgacggtgc ttacgtgacg atgaatcgga aatttattgt tcgaattgac acagaagagg
     3301 tagaagatcc gacagaaaaa gcacgttctc gaaaagatga gatcatgggt gtagtgaata
     3361 ctttgtccaa tatgggcttt tagcaaggat gctgaacaaa gaaatacaat aaataaacag
     3421 aattggattt cttcgcttag cttctttgct ctaggaatac atttaaaggt tgtgacaaaa
     3481 ttcgtgtcac aatctttttt tattttgaat aagtgatgtt caaaaaccat tttctcggta
     3541 ttaagccaaa tttatgcaaa aatgtgagaa actattttca caaaattttt cttcatactc
     3601 gaaactaatc ggcttcttca cgacctctta tacacggcgt tcaaaagctt gcatctgcga
     3661 taataacccc aaccaaacga aaaacatgag aagctgttta tgtttggctg ttcttattac
     3721 ttgatgcagg acagcttttt tacgacctct tggtacaggg tagctttttt accaatttct
     3781 agctataatt aaagataacc ttgaaagttg cagaaaaaaa gtgcaaaata aacgataagt
     3841 acaaaaagac tagttgaaag gacgtcgtca tgtcagagcg tggattatta atcgtgttgt
     3901 ctggtccatc cggtgtagga aaaggcacag tacgaaaagc aatttttgag agtgaggata
     3961 atgattttca gtattcgatc tcaatgacta cccgtaaaat gcgtgaaggg gaggtcgaag
     4021 gcgttgatta tttcttccga agcaaagaag agtttgaatc aatgatcgaa gctggagaga
     4081 tgctggaata cgcagaatat gtcgggaatt attatggaac tcctttgtca tatgtcaaca
     4141 agacattgga tgaaggaaaa gatgttttct tggagattga agtccaagga gcaaaacaag
     4201 tcaaagaaaa agtgcccgat ggtgtattca tctttttgac tccgccagat ttagcggaat
     4261 taaaatcacg gatcgtaggc cgaggaacag atgcagatga agtaatcgaa aaacgaatga
     4321 aagttgcaaa agaagaaatc gaaatgatgg ctctttacga ctatgctgtt gtaaatgatg
     4381 aagtgccgct agcagttgaa cgaatcaaaa acattattgc cagtgaacat tttcgagtag
     4441 atcgcgtaat cggtaaatat attaaaatgt taaaggagat gtagtcttat gatgttaaaa
     4501 ccttcaattg actcactatt agatcgtgta aattcaaaat attctctagt gattttagca
     4561 agtaaacgcg cgcatgaatt ggatgcaagt gcacagccta ctctagattc ttttgattca
     4621 gtaaaaagcg tcggtcaagc tttagaagaa atcgatgcag gaaatgtagt caatgatcca
     4681 catcctgaat taaaacgtga acgtttgaag atggaagaag aggaacgtca agcacaaaaa
     4741 gagcgtgaac aacacgaatt ggaatcacgt atccgtgaag agcaaaaaat gtaataaaga
     4801 aaaatagagc gaacatctaa aaagaaaagc aaacccagct gtttcctttt caaacattcg
     4861 ccacttattg tctggtagaa aaggctgtgt ctgaactttt tgacacagtt cttttttttc
     4921 ttatctgctg ttaatttttt gttaggattt ggcagaagat aagacaaatc catcgatctc
     4981 tagtaaaata ggcaaggaac tttttgtcgc ttgataagaa aagacaaaaa aaggaggaaa
     5041 cgaaccatga cattaacagc tgaagtcatt gtggacgtac ctactatgca gaccgatcag
     5101 ccattcactt atttggttcc ctctgaaatg gaaacagctt tgcaagtcgg gatgcgagta
     5161 gaagtccctt ttggtaatgg caatcgtcat gtccaaggat ttgttatggc tatccaaaaa
     5221 tcagaagaat cagctaatcc atctttgaag gcagttattc gtttgctaga ccttgctccg
     5281 gtggtcaatg aagaattact ttcattagct gattatatga aaaaaattac ttacgcattc
     5341 aaaatcactt gtctacaaac gatgcttccg agtgtgatga aagcagaata tgataaattg
     5401 atctatcctt tggcagacac accagaagtt caggtattgt ttgatcagcg agaagtgatt
     5461 tcctggaagg aagcagaaga agaaggcagc ctttctcaat tgattcgctg gcgccaagag
     5521 caattagtag atatcaaata tgaagtccat acacgaaata aagtaaaaac gattcgtttg
     5581 gtccgttctt tgttaactga aaagcaaatc gaagaagaat gggcaaaatt acggcaaaat
     5641 gccaagaaac aaaaagaact tcttttgtgt ttatctgaac tgagccaaga agaaccaatt
     5701 gcttatttca aaaataaagg aatcagtact gctgtcttga atcaaggaaa agaaaagggc
     5761 tggctggagt ttatagaatc agaacgttat cgcgaccctt ataaagaccg agtatttgac
     5821 caaacgacgg cattggaact gaatgcagaa caaaaaaatg ctgtcgaaca aatcatcaca
     5881 gcaggtcagc agcaaaaaga tcaggtcttt ttgctggagg gaatcactgg cagcgggaaa
     5941 acagaagtat atcttcaggc aatcgctgat gttttgtcag aaaataaaac ggctattatg
     6001 ttagtgcctg aaatcgcatt gactccgcaa atggtcgagc gattcaaagg acgcttcgga
     6061 gaatcagttg cggtcttaca tagcggactt tcccaaggtg aaaaatatga tgaatggcgc
     6121 aagattgagc gggaagaagc gcaagtagta gtaggcgcac gatctgctat ttttgccccg
     6181 ctgaaaaata tcgggctgat cattatcgat gaagaacatg aatcaagtta caaacaagat
     6241 gagacaccgc gttatcatgc gagagatcta gcaatttggc gcagtaaata tcatcattgc
     6301 ccaatagttc taggaagcgc gacgccctct ttagaatcaa gagcacgagc tcaaaaaggc
     6361 gtttatcagt tattgcagtt aaaccaccga gccaaagcag ctgcacagct tccagctatt
     6421 gagatcgtag atatgcgtga agagtttcag aatcatcgga cctctacttt ttctgctaat
     6481 ttgcaggaaa aaatacaaaa tcgattagac aaaaaagaac aaactgtcct actgctcaat
     6541 cgtcgaggat attcgtcttt tgtgatgtgt agggactgtg gatttgttct tccatgtcct
     6601 aattgcgata tctctcttac actgcatatg gatacacgtt caatgcgctg ccattattgc
     6661 ggacatgaag aacctattcc caatcgctgt cctaattgtg gcggcaataa aatccgctac
     6721 tatggaacag gtactcaaaa agtagaagag gaactccgag aactttatcc gcaagctagg
     6781 attttgcgta tggatgtaga taccactcga agaaaagggg cacacgaaca gattttacaa
     6841 aaattcggtg caaaagaagc agatatatta ttagggacac aaatgattgc taaaggtttg
     6901 gactttccag aagtgacatt agttggggtg ttgaatgctg atacctccct gaatttaccg
     6961 gattttcgtt ctagtgagca tacattccaa ttgcttaccc aagtttcagg acgagcaggg
     7021 cgagcggaaa aagcaggcga agtcgtgatc caaacattca atcctcaaca ttacgccata
     7081 gaattggcta aaaaacaaaa ttatgagcag ttttatcagc aggaaatgca tgtacggcat
     7141 cgtggaggct atcctcctta ttactttact gtgaaaataa ccgccagcca tcctgaagaa
     7201 caagtagcgg caaagaaaat ctttcagatt gccaatcagc taaaagaagt gttaagccct
     7261 caaagcttgc tattagggcc aacgcccagc atgattttga gagtgaaaaa ccgatattat
     7321 tatcaactaa tcatcaaata taaacatgaa ccgaatcttc ctcatgtttt ggatgaaatt
     7381 ttgaatggaa gccagaaaga acagcgacaa ggtctttttg tggcaattga caacgaacca
     7441 ttaaacttta tttaagaaag ggccaaatta tgcgctatcc tatattgatc caccctaatg
     7501 ataaattaaa aagaactgct cagccaatcg atgtaatcac ggacgagaca atcgctttat
     7561 tagacaattt atatgagaca atgattgcga atgacggaat agggatcgct gctcctcaag
     7621 taggacaaaa caagagaatt gctgtgatcg aagtagatga aggcgagaaa tttgaactga
     7681 ttaacccaga aatcatcgaa gcaaaaggtg aaagccttga cgtagaaggt tgtctcagta
     7741 ttccgcatgt ctatggaacg gtcaagcgag cagatgaagt gaccgtgcgt tattatgatc
     7801 gagacggaga agagatagaa gtgacggctt ttggctattt agcccgtgcc ttccagcatg
     7861 aaatcgatca cttagacggt atcttgttca ttgagaaaat gatacagcag atcccggaag
     7921 aagaacttga agaatatatg gaggaacatc ttgatgacta agctagtatt tatgggcaca
     7981 cctgcttttt ctgtgccgat tcttgaaggg ctgatagaat caggttacga gatccaggca
     8041 gtggtaacac agcccgaccg tccagttggg cggaaacgag taattacgcc gacaccggta
     8101 aaaaaagcgg ctctgaagca cggtatccgt gtgctgcagc cagagaaaat ctccggttcg
     8161 ccagaaatgg aagaaatcat tgaattggtg cctgatgtga tcgtcactgc ggcatttggc
     8221 caattcttgc ctgaaaagtt attgcaagta ccaaaattag gggcaatcaa tgtccatgct
     8281 tctttacttc ctaaatatcg gggaggtgcg cctgttcatt atgccatcat gaatggggag
     8341 aaagaaactg gtgtaacgat catggaaatg atcaaaaaaa tggatgcagg cggaatctat
     8401 gcgcaagaaa gcataccaat cactaaacaa gatgatgtag ggacgatgtt tgaaaagctt
     8461 agccttttag gaagaaagct attgctagaa acattgccca atattttgga tggacaaaaa
     8521 ccagtacctc aagacgagtc agaagcaact ttttcgccaa acatcacacg ggaacaagaa
     8581 gcaatcgatt ggcataaaac tgcagaagaa attgacaacc aagtacgcgg aatgcgtcct
     8641 tggccaattg cattcacgac ctatgagggc atacgctgga aattattatc ggttgaagta
     8701 ttggcagaaa ctacagacaa agcacctggt acgatcatcc ggaaagacaa aaaaaatcta
     8761 tggattgctt gcggtaaaca gaccgtctta gccatcaatg aattacagcc agctggtaaa
     8821 ggaaaacagg ccgtacatga tttccttaac ggaagcggtc agcaagttca gattggtcag
     8881 caggtgaaat aatgaaaaaa atccctaaaa aaatgaaaaa atcagtacga tatatggcat
     8941 tactgacatt agagcgagtg aacaagggtg gcgcctactc gaatcttttg ttgaatgaga
     9001 tgatcaaaca aagcgagcta aatcaaaaag atgtgggttt atttacagaa ttgatctatg
     9061 gtacgattag ccatcagcga ctattggaat tttacgttgc accattgatc gctaaagcaa
     9121 aaaaagtgga tgactgggtc aagatgcttc tttatctttc tgtataccaa ttagaatttt
     9181 tagataaagt ccctgcacat gctattttaa acgaggcggt cgaaatcgca aaagtaaaag
     9241 gaaatcctgg gacagggaaa tttgtaaatg gtgtcttgag gaattatcag cgtcaaggag
     9301 caccagatct gcaaggcatt gctgatccta tcgaaagact ttctgtcaaa atcagcctgc
     9361 ctctatggat gacgaaacga ctggtggatc aaatcggata cgaagaaact gaaaagctcg
     9421 gtttatcgct atatcagcct agccatgcta gtgcccgagt agatacacgc cgactttcca
     9481 gagatcaggc catcactgtt ttgcaagaag aagagatcac agctgaaaaa agccaagtca
     9541 gtccatacgg tatagtagcg aaaaaagggc atttagcttc tagctcattg ttccacgatg
     9601 gcgtcatgac gatccaagat gaaagttcga tgcttgtagc gccgtctatg caaatcgagc
     9661 ctgaacatca tgtcttagat gcctgtgcag cacccggagg taagacgacg catattgctt
     9721 cttttttaga tccgtcatgc ggtggagaag tggtcgcttt agatgtacac gaacacaaag
     9781 tcaaactcat tgaagataat gcagcacgtc taggtgtctc ttctgctgtc tacgctcaaa
     9841 agatggatgc tagagaagtc agtaaacagt tcgaagcaga gcagtttgat cggattttag
     9901 tagacgcgcc ttgttcaggt cttgggctgc ttcggcgaaa accagatatt cgttataaaa
     9961 aacagccgga tgaattagcg aatttaccaa aaatccaatt ggaaatttta gaaagctgcg
    10021 caccaacttt aaagtcttcc ggtattctga cttatagcac ttgtacgatc ttaaaagaag
    10081 aaaaccaaga cgttgtttct gctttcttgg aaagacaccc tgattttgag aggattgatg
    10141 ttttgaccaa tcctatgcta caatcatcaa tcgaagagaa aatgttgact ttgtatccgc
    10201 atcagtttta cacagatgga tttttcattt gttgtttgcg caaaaagtaa tgaggtgaaa
    10261 aaatgcagat tgaatatcaa tcagatgtag gaagacgacg taacacaaat caagactatg
    10321 ccagtgtatt taccagccgt tcaggtctca agcttgctgt attagcagac gggatgggtg
    10381 gtcatcgtgc cggagacatt gccagtcaga tggcggtaac gaatctaggc gaagcttggg
    10441 aaaaacaaga agctgttgac gatgaaaaaa tcgcccaatg gttcattcaa tcgattcaag
    10501 aagaaaatat gctgatttat cagcgtggac aagaacagcc ggaatataac ggtatgggaa
    10561 caacaatcgt agcggccgct ctgtcagatg actggttcac gattgccaat gttggggata
    10621 gcagagctta tttgattcgt gaagataaaa tcatgcaatt gacggaggat cattctttag
    10681 tcaatgaact agtcaaatca ggagaaatca gcgaagaaat ggcagtgaac catccaagga
    10741 aaaatatttt aacacgttct gtcggcatgc ctggaacagt cgaagtagat gtttctactc
    10801 atctttggca actaaaagat cgactgctgt tatgttcaga tggcttgacg aacatgctgt
    10861 ctgaagaaga aataggaacg atcatcaatc aagaaggccc attgtctcat aaagtaactg
    10921 agctgattga tcgtgccaat gaagcaggcg gagcggataa tatcaccgtt cttcttatcg
    10981 aatataagga ggacagtgta tgatcgagtt gggcaaaaaa ttaaatggcc gttatcaaat
    11041 cactggaaat atcggtagcg gtggtatggc aaatgtattt ttggcgcacg atttaatctt
    11101 ggatcgtgat gtagcagtta aagtcttacg atttgacttt caaaatgacc agactgcgat
    11161 tcgacgattt cagagagaag ctttagcagc aacagaactc gttcatccaa acattgttag
    11221 tgtttacgat gttggtgaag aagacaacat gcagtatctg gtgatggaat acgtcaaagg
    11281 aatggacttg aaacgatata tccaaacaca ttatcctgtt ccttatgaaa cagcagtaaa
    11341 tattatgcag cagatccttt ctgctatttc tttagctcat aaccatcaaa tcatccaccg
    11401 agatttgaaa ccccaaaacg tactgatcga taatgaaggt gttgtaaaaa tcacggattt
    11461 cgggattgct atcgctttat ctgaaacatc gatcacgcag acgaatacga tgctaggatc
    11521 tgttcattat ctgtcccctg agcaggccag aggaagtatg gcaacgaagc agtcagatat
    11581 ttatgcttta gggatcatcc tttatgagat gttgacgggt agcgtaccgt ttgatggtga
    11641 atctgctgta acaattgctt taaaacattt tcaagatgat cttccatcta taaaagcact
    11701 tgatccaaat gtgccacaag cactggaaaa cgtgatttta cgggcaacgg ctaaagaacc
    11761 tgcagaccgt tataagtcag ccgaggaaat gtctgatgat ctttctacag tattatcacc
    11821 agctcgtgca aacgaagaaa aatggcagcc tcatgtgatg gataatgaaa caaaagttat
    11881 cacacccctt acggaagatg caccaatgcc tgattcattc aaatctatgc ctctgccaaa
    11941 agacaagcca gaaaacgaaa ccttaccaga ggaggaagaa gttctgcctg atcctaaaaa
    12001 gaaaggacag aaaaaatggt ggctgatcct attagctatt cttgctgttt taggaatcgg
    12061 aacagctgta ttctttgctt ctggcgggcg aggagaagtt gttgttcctg atgtttctga
    12121 tctttctgaa gcttctgcta gagaaacact cgcaaaagcg aaattagaag tagcaacaaa
    12181 aacagaagaa attgcagatg acaaaatcga agaaggtaat gtagtgaaga cagacccggc
    12241 agcgggaacg acagtcaagc aaaaacgcga agtcactctg tatattagta gcggcacgaa
    12301 aaaaatcaaa ttagatgatt acagtggtaa aagttatgaa gatgccgaag caagtttgaa
    12361 agaattaggg ttttctgctg acttgattca aaaagaagag gaattcagtt cagaggtaga
    12421 aagcggaatg atcatttcgc aatctccatc agaagataca gaagtcgatc ctaaaacaga
    12481 cacgattact ttcacagtca gcaaaggacc agaaacagtg actttgagta gtcatgtagg
    12541 agaaaactac gaaactgttc gtaattggct tgttacacaa ggattcaata tcaataatat
    12601 tagtgctact tatgactata gcgacactgt accatcaggt tcgatcatca gtcaggatcc
    12661 agcctcgggt gaagttgtag cagaagaaac ttacatcaat tttgtcgtaa gccaaggaac
    12721 agagcctaaa ttgaatgata tctccggcta tacaaaaagc gaagcacaaa gttatttagc
    12781 tagtgtcggt gcagaatata tcgggcatga gacttccgaa tattcaaaca cggtggataa
    12841 agataaagtg attcgtacag taccaggggc aggaacgaca attaccaaag gaatggttgt
    12901 gaacgtaatt tactctaaag gaccagatcc agcgtcttct agttctgaaa aagactcttc
    12961 agacagttct agcagctcaa gtagctccag tagcacaagc aactcttcaa aagaagaatc
    13021 ttccagctcc acaagttcat cagctagcac ctcagaaagt tcaaaataag tgctgaataa
    13081 aatatatcag aagggaatgc gacattgatt tgttgcattc tcttttttgt gtttctatct
    13141 cttttgatca attttcaagc catatacttt gagaagtttg catggaataa cacctacttt
    13201 cctcttcgta tggtaaaata aacaaaaacg gaggtggcac ttatcaaagg acaaatcaga
    13261 aaagcaatca gcggttttta ctatgtatac gcaaacggag agacctatca aacaagagga
    13321 agaggaaatt tccggaatcg gaaaatcacg ccgcttgtcg gagatcaggt gatctttgag
    13381 agtgaaaatc aaacagacgg ctatcttctg gaaatcttgc cgagaaaaaa tcaattagtt
    13441 cgcccaccag tcacaaatgt tgatcttgga atcattgtca caagtatcgt agagcctgca
    13501 ttttcttata atctacttga ccgctttcta gttaccttgg aatacgagtc gatcgaaccg
    13561 attatttttt tgacaaagac cgacttgtca gacgacccag aaaaaatcga agagatcaag
    13621 caaacctata ctaaaatagg ctatccggtg attgtaccca atcatacggg tgatacgaca
    13681 gaactgatcc gttacttccc agaacggcta tcggtcttta tgggtcagtc tggagcagga
    13741 aaatcgacgc tactgaatca aatatcaccg gaactgaatt tagcaacagg agagatttct
    13801 gatgcacttg gaagaggacg gcatacaaca cgtcatgtgg aactgattcc tatctatggc
    13861 gggttagtag cagatacacc aggatttagc tcgattgact ttttgacaat tgaagcagga
    13921 gaactgccga gacaatttcc tgaatttgta gaagcttctg tccattgcaa atttagagag
    13981 tgcatgcatg ctaaagaacc tggctgcgaa gtaaaacata gagtagaaac aggtggtatc
    14041 gcacagacac gttacgacaa ttatttgcaa ttcttacaag aaatcgaaaa aagaaaacca
    14101 atgtatgcaa aaaaagaagg taaacgaaaa taggaggaaa caagaatgaa gatagcacca
    14161 tccattttga gtgcagattt tgccaattta caaagagaca ttgagttagt ggaaaaagga
    14221 ggcgcagatt acatccacgt agatgtaatg gatggccgat ttgtcccaaa tatcacatta
    14281 gggccaaata ttgttcaggc aatccgtcct gttactaaat taccgttaga cgtccatctg
    14341 atgatcgttg atccagaaaa ctacattcca gcatttgcaa aagctggagc agatatcatt
    14401 actgtccacg tcgaagcaac tcctcatatt catcgggcac ttcaaatgac aaaaagctta
    14461 ggagttaaag ctggagtagt tatcaatcca ggaacaccag tctccatgat caaacatgtt
    14521 ttgcctttgg ctgatcaagt actagtcatg actgtcaatc ctgggttcgg cggacaaagc
    14581 tttattgaag aaacagtaga aaaaattacg gaactcgctg atctacgtag agagaacaat
    14641 tggacttatg caatcgaagt agatggcgga atcgttccag aaacagcaaa aatctgtaaa
    14701 gatgcagggg cggatgtctt cgttgcagga tcatatatct atggagccga aaatccagtc
    14761 ggacaaatcg aacgattaaa agaggcatta gactaaatga aaattttatt agctgctgga
    14821 ggtaatccgc aaaactggcc gcagtctctg ctgtccaaat acgatgccta tgtcgggata
    14881 gaccgtggtg cgctgtacct gcttcaagca ggctacccag tagatctggc agtgggtgat
    14941 tttgattcat tgtcgaacga agagcagaag gaagtatttg caaaagccaa agagtatacc
    15001 cgttcccctg cagaaaaaga cgatacagat acacagttag gtatcttaaa aacatttgaa
    15061 cgttacccgc aagcggaagt cactttgatt ggagctacgg gcggaagatt ggatcatctt
    15121 ttagccaatc tctggttagg tatggaacct cgcttcaaac cctttgccca tcagcttatt
    15181 atcagagatt gtcaaaatta tttgtcttat tatttgcctg gagagcatat agtaagacag
    15241 ataccagata tgaagtattt agcttattgc tgcttgactc ccgtctcgaa tcttacatta
    15301 aaaggcagca agtatgtatt ggaaaataaa gaagtagaat atccgacatc ttatgcaagt
    15361 aacgaatttc tttcagatac cgcagaattt tctttttcag atggtatgat tgcagttatt
    15421 caaagcaaag attgatccat aaacaaataa aaaaatttgt ctttcaggct aaaaatgaca
    15481 gaatctatta tttgctaaaa aaaagcttaa gaggagcaac ttaccttttg tttcacggca
    15541 tttagtgata aaattgaaca caagagtcta attaattcga ggtgtctttg tgaagaagtt
    15601 caatcctaat aaaaatatta ttattacctt ggttattgtt atcatcgtgg tgacgatact
    15661 aagtgtgacc gctgcgcagc gagcgaatga aggaaagccc aatttcttcc aatccatcgt
    15721 taatgacagt gttgcaatgg ttgataaaac aatctctgcc ccagtacggt gggtacgaaa
    15781 tggagtagat tctgtccata atcttttttc aacttatgcc gaaaatgagc ggctaaaaga
    15841 aaaaatcgat agctatgatg acgtttcaca gcaaaacaag aattacgaga gagaaatcga
    15901 agcgttgaaa aaagaacttg agttgaatca gacgctgact agttacgaaa aagtaacggc
    15961 aaatgtcatt actcgttcgc ctgacagttg gcaggacatg ctgatcgtag acaagggttc
    16021 taaagacggt atcgaagcaa atatggcagt catgtcgcaa aaaggattga ttggccgagt
    16081 gatcgaggtc aatacggctt cgtctaaaat cgaattactg tcatcctcta atgaaagctc
    16141 caatcatttt ccagtacggg tatcttcggc taatggcgaa gcgtttggtt tgcttaaaaa
    16201 ctatgatgaa aagctccatg ccttagtggt gacccaatta actggtgata cggatatcaa
    16261 agaaggggat gttgtccaga catccggtct tggagggaat tctccagcta acttgccgat
    16321 cggtacggtt attaaaacga aaccagatag ttatgggctg gatcgggaag tttatgtgaa
    16381 accttatgca gaaatgtatg acgtgtcagt tgtgacgatt gtccaacggc tagcggagga
    16441 ttagtccatg ttgaagaaag aaacaatgaa gtactatctg ccaatcgttt tgttcttttt
    16501 gatgttgata gatggtcatt taacaagaat gctaggggag tggtcgaaag gcacctatat
    16561 gtcaaatgcc cactttctga tattggcatt attatgttgc agtatggcgt ttgaaaaacg
    16621 ttatttactg attaccacga ttgttctcgg ggctatctat gacgcttact atattggcgt
    16681 tatcggtatc tatgcagtag ctctcccttt aattgtatgg ttgatgtatg taatgaaaga
    16741 cgttatccat gtcaacatct ttactgaatt tttcagtatg atcatctttg tcacgggtta
    16801 tgaattgttt acgatggtgg tccagttgat ttttaaatta gcagtagtaa ataacacgta
    16861 ttttattaca aggtttttag gacctacact gctgttgaac atgattatat ttgtattatt
    16921 catttttccc tttaagaaat tattcagtga tgaataattt ctttttttat ttttaggtcg
    16981 aaagcgaaaa agaatcgttg ttatcttaag aaatgctaga cttaacgcgg ctttttcatg
    17041 atcattttct gaaacgaaca gttttgtcat ttttaaaagt tgttttgaaa catttttgta
    17101 acataactat tatatcgttg acacactgct atggtacaat ttttatatcg ttaagaaaga
    17161 cttaaatata tttagatatt tattaaatta agccagtcgg aggaatgaag agtgaaaaag
    17221 agtttaatat cagcagtaat ggtatgttca atgaccttga ctgccgtagc atcaccgatc
    17281 gctgccgcgg cggacgattt tgattctcag atacaacagc aagatcaaaa gattgcagac
    17341 ttgaaaaatc aacaagcaga tgcacaatca caaatcgatg ccttagaaag ccaagtttct
    17401 gaaatcaaca cacaagcaca agacctatta gcaaaacaag atacattgcg tcaagaatct
    17461 gcacaattag taaaagacat tgcagattta caagaacgta ttgaaaaacg tgaagataca
    17521 atccaaaaac aagcacgcga agcacaagta agcaacacaa gctcaaacta catcgatgct
    17581 gtattgaatg ctgattcatt agcagatgct attggacgcg ttcaagcaat gacaacgatg
    17641 gtaaaagcaa acaacgactt gatggaacaa caaaaacaag ataaaaaagc tgtagaagac
    17701 aaaaaagctg aaaacgatgc taaactaaaa gaattagctg aaaaccaagc tgctttggaa
    17761 tctcaaaaag gcgatctttt atctaaacaa gctgatttga acgtactgaa aacttcatta
    17821 gctgctgaac aagcaactgc tgaagacaag aaagctgact tgaaccgtca aaaagcagaa
    17881 gctgaagcag agcaagctcg tattcgtgaa caacaacgtc tagcagaaca agctcgtcaa
    17941 caagctgcac aagaaaaagc tgaaaaagaa gcacgcgaac aagcagaggc tgaagcacaa
    18001 gctactcaag cttcatctac tgcacaaagc tcagcaacag aagaatcatc agctacacaa
    18061 tcttcaatga ctgaagaatc atcatcagct acacaatctt cagcaacaga agaatcaaca
    18121 acacctgaat cttcaacaga agaatcaaca gcgcctgaat cttcagcaac agaagaatca
    18181 acaacagcgc ctgaatcttc agcaacagaa gaatcaacaa cagtgcctga atcttcagca
    18241 acagaagaat caacaacagt gcctgaatct tcagcaacag aagaatcaac aacagtgcct
    18301 gaatcttcaa caacagaaga atcaacaaca cctgcgccta caacaccatc aacagatcaa
    18361 agtgttgata caggaaacgg cacaggaagt agtactccgg ctccaacgcc aacaccaaca
    18421 cctgaacaac caaaacctgt aacacctgct ccagctcctt ctggaagtgt gaatggtgca
    18481 gcaatcgtag cagaagctta caaatatatt ggtactccat atgtttgggg cggtaaagat
    18541 ccaagtggct ttgactgctc agggttcaca cgctatgttt acatgcaagt aactggtcgt
    18601 gacatcggtg gttggacagt tcctcaagaa tcagctggaa caaaaatttc agtttctcaa
    18661 gcaaaagctg gagacttgtt attctggggg tcacaaggcg gaacttacca cgtagcgatt
    18721 gctttaggcg gaggacaata tatccatgct cctcaaccag gcgaaagcgt aaaagttggt
    18781 tcagttcaat ggtttgcccc tgactttgct gtcagcatgt aagaatgatt aatcaaaaag
    18841 agttagccca ttctggacta actctttttt tattgtgata aaatagaaat cagataaatt
    18901 cttctgtcaa ttccatgaac gtttggatat cttcagtcac catgtcgata cctgcttgcc
    18961 aaaaatctgg ttgggtaaga tcgacattca aatgtttctt cgcaagatct tccgtcgtca
    19021 ttgaggcagt atcgcgtagt aaatggatat attcttcttc aaaatttgct cccttttggt
    19081 tggcgtaagc atagatgccc aaactaaaga ggtagccaaa tgtgtatggg aagttataaa
    19141 acggtacgtc atcaataaag aaatgcaatt ttgcagccca gaaatatgga tggtaagtag
    19201 ctaaagctcc ttgatagcct tctttttgcg cctcaaccat catctctgtg atcttatctt
    19261 ctgagactaa tccttcttgc cgtgcttcat agaatcgatt ttcaaaaatg aatcgggaat
    19321 ggatattcat gaacatagct aaagcatttt gcagttttgt atctagcaaa ttgatttttt
    19381 cttcttttgt ttttgcgact tttaatgtag catcggctac gatcagttct gcgaatgtac
    19441 ttgctgtttc tgcgacattc atggcatatt cgcgatttaa agaaggtaaa tcccacatcg
    19501 tgctgctatg gaaagcatgt cctagttcat gagcaagtgt cgcaacctca ttaacagaat
    19561 tactgtaggt cataaagatc cttgattctt ttgtttctgg taactcagta caataaccac
    19621 caggacgttt cccaggacga tcttctgctt cgatccagct cttttcaaaa gcagattggg
    19681 caaatttagc catttttgga ctgaatttgt taaagttttc gataataaat gctgctgctt
    19741 cgtcaaaact aaaggttttt tctttcaaat cgcctaaaat aataggtgca tcttgatctt
    19801 gccattccat tttttcttta ccgaaaagat tagcttttct agtcaagtaa ttaacaaggg
    19861 gttgtttgtt tttctggatt gtatcccaca tgacagacaa tgtttctttc ttcaaccgat
    19921 tgtactctaa tggtttttgt agaaaatctg tgacaccatg aagttcatag tcagaaagtc
    19981 taaaaccatc taaatgattc aaagtatccg tgaagagggg tgccttttct ttccaggctt
    20041 cttcccatgc tgcgaaaagt gtttctctga cttttggatc tggatcgccc atcatcttat
    20101 tgaaggcttg tccagcagat aattcgacta cttgtccatc ttgttcgaag ggaatcgaaa
    20161 tgctggctac aatcgtatca taatgactgc tccaagcatt tagaccatct aaagaaagcg
    20221 tatttataat gttttcttca gcttctgata ataattgtga gccatcacga cgaatctcgt
    20281 ttaaacgaaa agcaattgtt tcaaacgaag attgagaaag catctgattc caatcattgt
    20341 cagatatttc tgcaaatttc tttgacaata cagtttcggc tgattgcaga cgaggcagca
    20401 ttgcgtaaag cttgcctgat aagacctttg catctgaatc attgacattg gcagaaagca
    20461 atgcagtaat atagctgctg cattgggtaa aaccattcgt aatcgtttct tgcaattgaa
    20521 gaatagcgtt taactgctct gcattgtcag aactaggtga ccattttgtt acgcggtgat
    20581 aatactcgtt catttggtct tccaattgct tcatccgttg gttcaaagca tcagaatgac
    20641 tgcctccgga aaaaatagaa tctagattcc aatttaatga ataactcatg aaaaaactcc
    20701 cttcatattg taatacaaaa ttaagtatag cactttttta tggaagaagt ctctatgaaa
    20761 atgattgttg tttcttcaaa aaaagtctaa gataaaatac agaggtgact tatgaattct
    20821 tcaaaaaaac aaatgaaaaa cggaaatatt tatttagtaa tcgcactgat agtcgttata
    20881 atcttatttt acagttcttc tcaaacttac ggacagcaat cacaagtttc gcgattagaa
    20941 agctggctgc ctaatcagcc atttaaagac acattagcgg gtatacgttt taattatggt
    21001 ggaagtatgg tgagtatcaa ccatctgggc tacgccaaat ttttagagtt ctttttaagg
    21061 aagggcgcac atttcggtac ctattttatt ttgggaggca gcttgttttt aggtgttttc
    21121 cccaagctaa aaatttggtg gctgacagcg atccttgctt ggctttcagc aacagggtac
    21181 gcaggtttgg acgaattcca tcagatgatg accggtggac gaacacctat gttccaagat
    21241 gtcatgttgg attcgatcgg tgctctgact gctgtgatat tatgtgtgct ttttgcggca
    21301 ttcaagaagc gtttatagat tggctgtggc tttgtttttt tgtgaaatag tactagaata
    21361 gaagtgtatt ctcaaaagaa caagggagga aaaatcatgt caggacatag taaatggcat
    21421 aatatccaag gaagaaaaaa tgcccaagat gcaaaacgag ggaaagtctt tcagaaactt
    21481 tctcgtgaaa tttatatggc agctaaagca ggcggtcctg atccatctat gaatccagct
    21541 ttgcggcttg ttgtggacaa agcaaaagct gcaaatatgc ctaacgacaa tgtggaacgg
    21601 gcgattaaaa aggctacgag tgccgtagat gaaacgaatt acgatgaaat cacgtatgaa
    21661 ggatatggtc cgggtggagt aggaatctta gtccatgcat tgacagataa ccgtaatcgg
    21721 acagcgacaa atgtccgagt agcttttact cgaaacggtg gatcactggg agaaacgggt
    21781 tcagtcagtt acttgtttga ccgcaaagga tatatcgcta ttgaacgcga tgggttgact
    21841 gttgatgaag agacgatgtt tgaggatgta ttagaggcgg gggcagaaga tctgcaaact
    21901 tctcctgaag tattcgagat ttatactgca ccagaagatt ttgctgctgt acgagatgag
    21961 ttggaaaaag agttcacttt ggcgcaagca gaattgacga tgattccaca aacgacgatt
    22021 gatttaaatg aggaacaaaa agagcaattg gaacgtctag tagataagct agaagacgat
    22081 gatgatgttt cagaagtctt tactgctgct gaaatgtaag tagcacattg ttagataata
    22141 aaaagaaagc acctgtacca atcgtacggg tgctttcttt ttatatatta tttttgttag
    22201 aagaccaata caggcgttcc tttttcgacc atcccgaata attccttcat tacacttggt
    22261 ggggtgttga tgcagccgtg tgaaccgcgg gttttccata agtctccacc gtattcaggt
    22321 tgccagtctg aatcgtggat accaacacct gtccagtcaa tcggcatcca gtagtttacc
    22381 ggactctcgt aaggtgttcc atcgtcattt gttcctttta aagttgcatc ttcttcttta
    22441 ttccaaacat agaagactcc agcaggtgta ggtgtagttg gctttccgga aacgatatct
    22501 gtttcaaggg ccacttttcc gtctttgtaa taccacatat gctggttctc cagatcaact
    22561 tcgatatacg tatcttcgat caatggatga tcagcggttg tgccaccttg cacaatgggt
    22621 gaacgagtaa aatcttggcc tgccagaatc gcttttttca atgcttctgt ctcactgtct
    22681 gtttggatcg tccaactgta agtgccaact gggaccgtaa cttctcctct ttttgtactt
    22741 ttaaattttg tgtcattcgt acttgtgtta tactttgtgc ctaaatctgt tacatattga
    22801 cgtacttgtt ctgtatctaa atcgactttt ccatcattgt aggtcagcca actcataata
    22861 tcacttgatg ggatctggaa agtctcaccg tttatactgt aagtagcttt tacatttgcg
    22921 atagcattca tggatgcgag ttgttctttc agtgagctgt cttcagatgt tacttttggt
    22981 ttttctttga attcagtcaa ttcaattgta tctttaccag agtttaccgc actttttaat
    23041 tgtttgaccg cagcatcgac gtcgatcgta tttccattga cttcaggctt gataacgaaa
    23101 gagtcacctg ctttttcgat cgttgcatca gtagtaggtg tccggtcttt gttcaaatca
    23161 gtcagcttag ttgtcaatgt ctgaacggta gtgtctagtt tttggtggtt ttgactgaca
    23221 ggatctattt cttgtttttc tgccgctgag acataagtca ttccccaagt ccatccgttt
    23281 tgttcattta ggatcggaga aagctccgaa gtgaaatcat ctttgtagcc gagctcggat
    23341 tttggtactt cctgccagac ttttccatct tctttaatag ccagtttttt atcagaataa
    23401 gcttctttta atttttcatt tgctttttcg actgttagat tgctgacatt tattccattt
    23461 atttctgtat tcggcaaaaa atgctttgtg tagtatgtac tgcgataggt ataaaagctg
    23521 atcagcacta agagaacacc aagaactgaa agcaaagcaa catgtttcga tttttttcga
    23581 tgcgaagatc ttgtcatatc gacaactcct taagtaaaag tttagggtgg aacggtctca
    23641 tgatagcata atttagagga aaagacgaag gttatctcta ctttatgaaa aaatttagaa
    23701 accataaggg tgataaaata aaaaaacgga aaacaccgaa tagatgtttt ccgtcatgtt
    23761 caaagattat tcagcgtcta gtttagcaaa agcttcatct agaatttctt ttaattgctt
    23821 agcagaagca gccatacggt cttgctcgct gtcacttaaa ggaatttcga ttactttttg
    23881 gataccttga cggttgataa cagcaggtgc accaatgtag atgtcgttca atccatattc
    23941 gccattcatg aatactgata aaggaagaac agcattttca tcatctaaga tggcacgagt
    24001 gatacgtgct aatgctactg cgataccata gaaagtagcg ccttttttct cgataattgt
    24061 gtaagcagcg tcacgtacgc caaagaatag gttaaccata gcttcttcat cgatttctgg
    24121 gttgtttttt acccattcat agatttgcaa gccggcaaca tttgcatgtg accaaactgg
    24181 gaattctgaa tctccgtgtt cacctaagat gtaagcatgg acgttacgtg catcaacgtc
    24241 aactaattcc gcaagtgctt gacggaaacg agctgagtca agagaggtac ctgaaccgat
    24301 tacgcgttct ttagggaaac ctgagaattt ccaagttgag taagtcaaaa tatctactgg
    24361 gttagcggca actaaaaaga taccgttaaa tcctgattca acgatttgtg tgacaacatc
    24421 gcggttgatt tttaggtttt tgtttactaa gtctaaacga gtttcacctg gtttttgtgg
    24481 tgcgccagct gtaataacaa ctaagtcagc atcatgtgcg tctgcgtaag tagcagaata
    24541 gattttttta ggtgaagtaa atgccaaagc gtgagacaag tcaattgcgt ctccttcagt
    24601 tttagcagta ttgatatcca caatacctac ttcttgagcg atattttgag taactagagc
    24661 aaaggcatag ctagaaccta cggcgccgtc tccaacaaga ataacttttt gatgatcttt
    24721 tttttctgca gctgcagtca tttagtatca atccttccat tgatttgttt tttcccatgc
    24781 ttatgctaac atttctcaag ccccatgtca cgctatttgg ctataaaata taacagataa
    24841 caaggttgta ttatatgtaa aagcttacat gtgcgttatt tcactatttt tatatatcaa
    24901 ttatttcatt aaactttgaa gaaattttta atttctttat catttattaa cgattctctt
    24961 ttagccgaaa cgccttgttt tgtgttatac tatgcattga caagtgtaaa aaagaaaaaa
    25021 gctcgatgga ctggaccgct aattagcagt ccagcctttt gtgctgcttt ttagaattag
    25081 gtggaaaaca aaaatgaaaa tgatcgtcgg gttaggaaac ccaggtacca aataccaata
    25141 tacaaaacac aatatcggct ttatggtcgt ggataaaatt gcacgcgagc atcaagctac
    25201 tttcaaaaaa aatccgttcg aagcagaagt tgccgaattt tttcataatg gagaaaagat
    25261 ccttttagtc aaaccacaaa cattcatgaa tgaatctggt cgtgcagtcg gcccactaat
    25321 gacttatttt gggatttatc cagaggaatt agtcgtaatc tacgatgatt tggatctggc
    25381 agtgggaaaa atccggctcc gtcaaaaggg aagtgccggc gggcataacg gtatcaaaag
    25441 tattatttcc catttgaata cgaatgtctt tgatcgaatc aaggtgggaa tcggcaggcc
    25501 agaaggaaaa aaaacagtag tccagcatgt gcttagtcct ttcagcaaag aaaatcagcc
    25561 tttgatagaa gaaagcatgt gtcaatctgt taaagcagtc gaatatctca tagaagggca
    25621 ttcgttcgtg gatgcgatga atcgattcaa ttgaggaagg aggaaaagga atggatatta
    25681 ttcaattagt cggaaaagat ccgcaagtaa aagaatgggt cagcaattta gaagaaaaaa
    25741 tgcctagaca actaatcaca ggattagcgg gctcagcgaa aacacttcta tttgctcgag
    25801 cgtttaagaa aacaaataaa aacatgatta ttctgatgcc aaatttgtat tatgcgaatc
    25861 agttagcaga agatctgcaa catgtgattc cgcaagatca gatccattta tttccagtgg
    25921 atgaagtgct ttctgctgaa atggcctttt cttctccaga agcgcgaggc gagagagttg
    25981 caacgcttaa cgcacttcaa gaaaaaaaag caggtatcta tttgttgcca gtcgcatctt
    26041 tgcacaaacg actgccgaat aagaaaactt ggctacaggc gcaattgaat tggcatattg
    26101 gtgatgagat agacgtggaa aatcttcctc gccaattgac tttgatgggc tatgtacgag
    26161 aagagatggt cgcaaaaccg ggagaattca gtattcgagg aagcattatc gatatttacc
    26221 cgttaacgac aacctatcct gttcgtgtag aactatttga tgtagagata gattctatgc
    26281 ggtattttga agcagaaaca caacgctcaa tcgaaaaaac ggaagaaatc tgggtttccc
    26341 caacgagtga acaagtctac ggaccagagg atctagaaca tggaatagag caaatgcaaa
    26401 aaatgctaga aaaacggctt gctgcgacca cagaagaagc agaccgcgag tttttgcaag
    26461 attacttcgg tcagctgact tcaagctggc aagcaggtgt cccgacagaa gaagcaaaat
    26521 tctactcaga tcttctttac caagaatctg tgacgatctt ggattatttc ccttcaaata
    26581 gtttactagt cgttgatgat tatcagcgaa tcatggaaac aaatcgggaa atcgaaaggg
    26641 aagcggcaga atggcagact caaaaaatca gcgaattgcg ggtgttttct gagcagacat
    26701 tttcagcaga tatacaaaaa atcgtgcaga aggaaaagtt caccacaacc tttttctcac
    26761 tcttccaaaa agggatggga aatcttcgtt tccaagcaat ccataatttt caatatcgga
    26821 cgatgcaaca gttcttcgga caaatgccat tgctgaaaac agaaatggac cgttggcgca
    26881 agcaaaaaca gactgttgtg atctttattc cgacaaagga cagaatacgc aaagcagaac
    26941 aaatgcttcg agatgaagac atattagtag tagaaacaga aaaaaatgca ttgatcagag
    27001 gacaggtaca attagttgaa ggagctttgc aggctggttt tgaacttcct caagaaaaaa
    27061 tagtagctat cacagaaaaa gaaattttcc aaaaaacgac gaaaaaacaa acgagacgtc
    27121 aaacagtaac aaacgcagag cgattgaaaa gctataatga gttaaaagca ggagattacg
    27181 ttgtccatgc caatcatggt atcgggaaat acattgggat ggaaacgcta gaagtagatg
    27241 gcgttcacca agactatatg actattttgt atcaaaacga cgacaaactg tttattcctg
    27301 ttactcaact gaatctgatc caaaaatatg tcgcctctga agccaaagcg cctcgcatca
    27361 ataagctagg cggaagtgaa tggacgaaaa ccaagcgaaa agtctcttca aaaatcgagg
    27421 atatcgctga cgatctaatc aaattatatg ccgcaagaga gtcagaaaaa ggatatgctt
    27481 ttggacctga tgacgcctac caaaaagaat tcgaaaatgc cttcccctac tctgaaactg
    27541 atgaccagtt gagaagtgca gcggagatca aacgggatat ggaaaaagaa aaaccaatgg
    27601 atcgactact cgtaggtgat gttggttatg ggaaaacaga agtagcgctt cgagcggcct
    27661 ttaaagcgat aaaggaaagc aaacaagtag cctttttagt tccaacaacg atcttggcac
    27721 agcagcacta tgaaacgatg ctggaaagat ttgaaggttt ccccgtaaat atcgggctat
    27781 taagtcgttt cagaacaaga aagcaacaaa aagaaaccat cgaacaactg cgtacaggtc
    27841 aagttgatat tgttgtagga acccaccgaa ttttgtcaaa agatattgaa tttagcgatt
    27901 tgggtctact gatcattgac gaagaacaac gttttggtgt aaaacataag gaacgattga
    27961 aacagttacg tgcacaagtg gatgttttga ctctgactgc aacaccgatt ccgagaactt
    28021 tgcatatgtc gatgttaggc gtgcgggatc tttcagtaat cgaaacaccg cctgcgaatc
    28081 gttatccgat ccaaacgtat gttatggaaa aaaatcctgg ggctattcgc gaagcaatcc
    28141 atcgagaaat gggacgaggt ggacaagtat tctatttata taatcgggta gaaacgatcg
    28201 aacaaaaagt agaagaaatc caagaactcg ttccggaagc acggatcggt tatgctcatg
    28261 ggcaaatgac agaggcgcaa ttagaaaata cgctttttga atttattgaa ggtcaatatg
    28321 atgttcttgt cactacaacc atcattgaaa caggagtaga tatcccgaac gcaaatacgt
    28381 tgttcgttga gaacgctgat tatatgggat tatccacgtt ataccaattg cgagggcggg
    28441 taggaagaag cagccgtgtc gcatatgctt actttatgta tgagcagcag aagatcctta
    28501 acgaagtcag tgaaaaacgt cttcaggcaa tcaaagattt tacagaactg ggatctggat
    28561 tcaagattgc gatgcgggat ctttccattc gcggtgcagg taatctctta ggtgctcagc
    28621 agcatggatt tattgatgca gtcggtttcg atatgtattc tcaaatgcta agcgaagctg
    28681 tttctcgtaa acaaggaaag aatagtcaag tggagaagac cactgttgag atcgacctcg
    28741 gtgtcgatgc gtatctacct gaaacatacg tggcagatca acggcagaaa atcgagatct
    28801 ataaacgaat tcgtgaactt gattcgcaag aaatgctaga tgaactagaa gatgatctgc
    28861 tcgaccgttt tggagaatat ccagaagaag tagcacatct cttgacaatt gggcagatca
    28921 aaatggatgg cgatcgagcg ctcctagaaa cgataaggaa acaggaggcg catatagtct
    28981 ttactctaag taaagttgga acaaaacgct atagtgtgga acagttattc gaagcactga
    29041 cagcgacaaa actaaaagcc agtctaggcg ttgaaaaaga acagatggtg atacgcttga
    29101 cgatccccaa ccaaatggaa gaagcagtat ggcttcagga aatccaaaag tttgtcaaag
    29161 cgttgcgtga gcaaaaatat ctgtcagcgt aggaaggagc aatgatttgg atcataaaga
    29221 aatgcggcgc gtcatgcaag gagcatttgt attgacgatt gcttctttca tagcaaaagt
    29281 attaagtgcc ctttaccgaa ttcccctcca aaacttagtc ggcgacgaag gtttttatgt
    29341 ttatcagcaa gtttatccaa tttatgggat agcaatgact ttagctttat ccggtcttcc
    29401 gcagttcata tcaaagtacg ttgcagaaag aaagaatcct ttcgagcgaa agcaagcttt
    29461 gcaaaatctt tatcccttag tattttggac aggtgtcttt ttgtggttgt ttgtctttgc
    29521 atttagcagc tggattgctt ggatgatggg agacatacaa ttgaaaccgc tgatccaagt
    29581 agtttccttt acctttttgc tgatgcctgg tctatctttt tatcgaggga atttccaagg
    29641 aagattcttg atggaaccaa ctgctgtttc tcaagtagtt gagcagttcg ttcgtgtttt
    29701 tgtgattgtc tgtagtgcgt ttgcttttag aaagcttgga tggacggtgt accaaacagg
    29761 gacggcagca atgagtggag ctgtttttgg cggtttatgc gcatatggga tccttcacta
    29821 ctatgaacaa aagattcatg gaggagcgtt aagcttccga cggtttccat tgttcaaaca
    29881 gccgtcaaag cccttgatcc ggcgttttct ggtagaaggc ggactggttt cgatttatag
    29941 tggattgctt atctttttcc agctgatcga ctctttcttt gtaaaaaatg ctttggaagt
    30001 ttacggactg tcagaatacg gtgcgaagat agcaaaaggg gtctatgatc gaggtcagcc
    30061 tttagtacaa ttaggtttag tgattgccac tgcattaagt gcgacattcc tgcctgcgtt
    30121 gactcgtcat ttgaccaacc ggatctatcg tcagttttta cagacagcca aaatctatct
    30181 tagattgacg accgctttag cattagctgc gtctttggga ttagccttgc tgctccctta
    30241 tatcaattat gcgttgttta aagattatgc gggaaatgca gcgcttgtat tatttgtttt
    30301 ttccattgct ttcactgcag tcatccaagc ttatcaaagt attgctcaaa gcaagaactc
    30361 gtttcgccct tctttaaaag gcgcaggatg gggactattg gtaaaaggat tgactacttc
    30421 ttttctgacc ggtttattag gaacagcagg tgcaagcctg tctacccttt taggattagg
    30481 tgtgacgctt tggtattttg ttcgaattga aacaaaagag ctaaatgctt tttggaaaga
    30541 acggcatttt ggacggaagt tgatgttcag ccttctttgt atgacagtag tactgctgct
    30601 gtattatggt gtattgaata tagtatttgg tcctgtaact catcgttcca ctgctttttt
    30661 tgtcagcgtg ataggagttg ggatcggggc ttctgtgttc atttatgcag tgattcgtct
    30721 tcgtctgttt acagtacgag aatggctaat gttgcctttt ggtaagaaaa tcctgcgatt
    30781 aaataaaaaa attaaaaagt gaggaataag atatgcgatt agataaattt ttaaaaattt
    30841 cccgaatcat caaaagacga tcagtagcaa aagaagtagc tgataaagga agaattcaaa
    30901 tcaacggtaa attggcgaaa tcttccagtg atgtcaaagt aggcgatatt ttgaaagtcc
    30961 agtttggcaa caaaacgttg gaagtgaaaa ttacggaatt acatgaatca accaaaaaag
    31021 aagatgcaca aaaaatgtac gaaattattt ctgaaaatcg cacagaaaca ctagagaaat
    31081 agattagaca agctttcacg atattggtat aatgaaaaag acaatcaaaa agattggagc
    31141 gaatccaagt gacgaaaaaa agcaataaaa tcgctgcgct agatacggaa tatgcaaaga
    31201 aaaagtatgc tgaatttcag caacaacaac gtcaactgat ttttcgaaga cgcagactgg
    31261 ctgcaatttt tgttggtgct ttggtcattt tcgcgataat aggtgtgcag attttccatg
    31321 acatgcaaag gatgcatcaa ttgaacgaat tgagagtcga agcaaatact gaaatgaaag
    31381 aagtcaacgc agacgttgat cagctgcacc aagatgtttc attgctgaaa gatgatgagt
    31441 atgtagctaa acttgccaga agcaaattct tttactcaaa agacggtgaa aaagtttatc
    31501 ctatattaga atcaaataca gacagctcga caaatgaaac agatcaatca actagttcta
    31561 caactgcaaa ataagcagga atataaaaaa gggaaataat taggaggaac atttttttta
    31621 tgtcaatcga ggtaggagca aaactgcaag gaaaagtatc aggaatcaca aattttggtg
    31681 cgtttattga tctaggtgct ggaaaaacgg gtctagttca tattagcgaa gtgtcaaatg
    31741 gctatgtaaa agacatccat gatgttttaa gtgtaggaga tgaagtaaca gttaaagtta
    31801 tttcagtaaa tgatgatgga aaaatcggac tatctatccg gaaagcgcag gaacaacctg
    31861 ctgaatctgg agaagaaagc cgtcctaaaa aatatcaaca aaaagatccc cgtgacaacc
    31921 gaaatcgtcc agcacagaaa aaatcttttt ctcgcagtca accttcagct caaaaagaag
    31981 acttcgattc attgatgagt tcatttttga aagatagcga tgaccgctta tcttcattga
    32041 agcgaaacac agaaggaaag cgcggtggcc gtggcggacg tcgcagctaa aaagccgcaa
    32101 ataatgatag tgcgactatt tgggtcatag tatcaacaga gggcaggtta attgtcgttt
    32161 tagacgtttt cccgctctct tttttttagg aagaaagggt gtggaaagga tgaacgttga
    32221 aaaaggcggg aaaaaagcac cgctattaca gcagtcattt attcggcacg ggaatgaaca
    32281 aggattctgg caacaaggat cgagaatttt agcagcggta tcaggtggtg ttgattcgat
    32341 ggttttgctc catttacttt tacaagtgca acgccaaatc ggtttttctt taggtatcgt
    32401 ccacatcaat caccaattaa gagaagaatc agcagaagaa gctgcctatc ttcgctcata
    32461 ttgtgaagag aagaatctcc ctttatatgt aagtatttgg gaagatccag ccaaatccgc
    32521 catagaaaca gaagctcgta actttcgtta tcaagaattc actcgcgtga tggaagaagg
    32581 acaatatgac acgttgatga ctgctcatca cggagatgat caaatcgaga cggtgttaat
    32641 gaaaatcgtt cgtgggggac agttaaatac tttttctgga atcaaagaag tacaaccatt
    32701 tgctacgggc agactgattc gtccgttgct gagttttagc aaagaagacc tatatactta
    32761 tgcagcggaa tcgcagcttg tttactttga ggatcagaca aaccaattgt tagatatcca
    32821 gagaaatcgg cttcgtcatt tggtagttcc tcaattgaaa caagaaaata cacaggcgtt
    32881 gagacatttc cagcaatttt cgcaacagat ccaatgggca gaccaagtca ttcaaaaata
    32941 tatgggacag ttgatcgaaa aggaagtcga acagtgggag gaacaatttc gattttctgc
    33001 agaaatgatt gaagaaatga cagatgctga acgatactat tttttttact cctttttcga
    33061 caaagcattc tcaaaaacag gaattatttt gaaagaacaa caaatggagt cgattttaga
    33121 ccagtggcaa cagaaaaaaa gccaatggca actcagtatt ggcaataatt ggatactgaa
    33181 aagagagtat cattttgttg tactctctaa aaaagaaaaa caagcaacta gccagacaga
    33241 agaccagcgc ttgttgctga ttccagaaca aggaatatat ttgaatgaaa cagaatggat
    33301 tggtctggtc aaacctgaaa atgcagggga catcaaagca acagattcat ggtctctatt
    33361 ttctcatgac atttggattc ctgctgatgt gcaactgtat gtcgaaaaaa gaaaagcagg
    33421 agaccgcatt aaattaacag agaatctcac aaaaaaagta tctagatatt ttatcgatca
    33481 gaaagtacct aattcacaaa ggcagcatgc ttggatcatt acagatgcca aaagaaatat
    33541 atatagcttg ataccttttg ccttttcata tttgagtatt cgtgaagaaa ctgataaaat
    33601 acactacata cttctttaca aatatcaaaa agaagcaatt ggaaggagaa cctgatgttg
    33661 gaaaaggata ttgagagaat tttgatctca caagaagaga tacaagttcg ttgcaaagaa
    33721 ctaggcaaag agttaacaga aatttaccaa aacaccaatc ccctagtagt aggtgtctta
    33781 aaaggagcgg taccattcat ggcagatatc gttcgttcta tcgatacgta tttggaattg
    33841 gatttcatgg atgtttcaag ctacgggaat gcaacagtct cgtcagggga agtaaaaatc
    33901 gtcaaagact tagatacgaa tgtagaaggt cgcgatctgt tgatcgttga agatatcatt
    33961 gacagcggaa gaacacttgc ttatttagtg gatcttttca aataccgcaa agcaaaatca
    34021 gtcaaaattg ttacactact tgacaaacct gaaggaagag tagtaaatat cgaagcagat
    34081 tatgttggct tcaacgtacc aaatgaattt gtggtcggtt atggcttgga ttatgcagaa
    34141 gcatatcgta accttccgta catcggtgta ttgaaaccta gtgtttatca atcaaattaa
    34201 tgctgtatct caattaaatt atggtacaat agaatggtca ggattgataa aaaactgtta
    34261 aattaaattg acgcttaggg agggcaacta tgaacaaaaa gaacaacggc atgatgaaaa
    34321 atgccttgta ttatgtcctc gtaatcttag cgatggtaat ggtcgtttat ttcatttttg
    34381 gtaataacgg ccagcgatcg tcagatatcg agtactcaaa attcacagaa caactacaag
    34441 caggcaagat caaagaattt gaagtgcagc ctgcaaatgg tgtatataag atcactggtg
    34501 aatacaaaga acagcagacc gtcgctaaca gcggtgggtt agctatctta ggttcaacag
    34561 atacgactac tacccatttc acaacaatca ttttgccaaa tgatacaaca ttgagccaag
    34621 taacagaact ggcaaaagaa aacaaagttg ctacaacagt aaaagaagaa tcaagcagcg
    34681 gtatatgggt ttccctattg cttagcttct tgccaatcgt aatcatcatc tttttctttt
    34741 acatgatgat gggacaacaa ggcggcggcg gtggcggtgg cggccgtgtc atgaactttg
    34801 gtaaatcaaa agccaaagaa gcagacaaaa aagctaatcg cgtacgtttt tctgatgtag
    34861 cgggtgcgga agaagaaaaa caagaactag ttgaagttgt tgaatttctg aaagatcctc
    34921 gccgatttgt tgaattaggt gcccgtatcc cggcaggtgt acttttagaa ggacctccag
    34981 gaacaggtaa aaccttatta gcaaaagctg ttgccggaga agctggagta ccgttttact
    35041 cgatctcggg ttctgatttc gtagaaatgt ttgtcggtgt tggagcaagt cgtgttcgtg
    35101 atttgtttga aacagcgaag aaaaacgcac cagctatcat tttcattgat gaaattgatg
    35161 cggtaggtcg tcaacgtggt gccggtatgg gcggcggaca tgatgaacgc gaacaaacat
    35221 tgaaccaatt gcttgtagaa atggatggct tcgacggaaa tgaaggagta atcgtcattg
    35281 ctgcaaccaa ccgttctgat gtactagacc cagcgttact acgtccaggc cgttttgacc
    35341 gccagatttt agttggacgt ccagatgtaa aaggtcgtga agcaatcttg cgagtccatg
    35401 cacggaataa accgatggca gatgacgttg acttgaaagt tgtggctcaa cagacaccag
    35461 gttttgccgg agcagattta gaaaatgtct tgaatgaagc agcgttagta gctgctagaa
    35521 gaaataaaaa gaaaatcgat gcttctgata tcgatgaagc ggaagatcgc gtgattgcag
    35581 gtccagctaa aaaagatcgc gtaatcagca aaaaagaacg cgaaatggtt gcttatcacg
    35641 aagctggtca tacgattgtc ggattagtat tgagtcgcgc tcgggtcgtc cacaaagtaa
    35701 cgatcatccc tcgcggacgc gccggcggat atatgatcgc tttaccaaaa gaagatcagt
    35761 tcttgatgac gaaagaagat atgtttgaac aaatcgtagg cttacttggt ggacgtacag
    35821 ctgaagaaat catcttcaat gtccaatcaa ctggtgcttc caatgacttt gagcaagcga
    35881 cagctctagc acgaagcatg gtaacagaat atggaatgag tgatcgttta ggtcctgttc
    35941 aatatgaagg aaaccatcaa gtgtttgttg gccgcgacta tggtcaaaca aaagcttact
    36001 cggaacaagt cgctttcgag attgatcaag aagtacgaaa aatcttgatg gaagctcatg
    36061 ataaagctcg agaaatcatc gaagctcatc gtgcacagca taaactgatt gctgaaaaac
    36121 ttttggaatt cgaaacattg gatgccaaag caatcaagtc attgtttgaa cacggtgtga
    36181 tgcctgaagg tactgagtca aatgattatc caagcgaaaa agaagcgaaa acttttgaag
    36241 aagcaaaacg tgctttggaa gaaaaagacg ctgaaaaaca agcggaagaa aaacgtgatt
    36301 ttgaagaagc aaaacatgaa ctgaatgagg aaaataaagc agaacatgat tcagattctc
    36361 aacaatcaga agataatcat tcagacgatc aatacaaata aaaaaatata ggtcaattga
    36421 tctgatatga ctcccgaaaa ccggaacaaa aaccggtttt cgggagattt ctttatttaa
    36481 gttgtaaata gggtgatgtc ctgttttcta gatgattctg aattattttt aaaagaagca
    36541 ttccaaagtt gaaaaaagca gtagttgcta gtatgattca ctatgagttt aaaaacggaa
    36601 aaaggagtac ataacaaatg aaagattatt tggttaaggc acttgctcac gacggattcg
    36661 tccgagcata cgccgttcaa gcaacaaata ccgtagcgga ggcacaaaga cgtcatgata
    36721 cttggcacac ctcctcagct gctttgggac ggacattggt cggtagtctg ctacttggtg
    36781 cgacgctcaa aggagaagac aaactaactg ttcgaatcca aggcgatggt ccagcaacag
    36841 gaattgtagt agatgcaaat ggaaagggag atgtgaaagg ttacatcaaa aatccgcatc
    36901 tcagtcttcc tctaaatagt gaaggtaaaa tcgatgtgcg gggagcagtt ggaacacaag
    36961 gaatgtttac tgtcacaaaa gacttaggat taaaagaacc tttttctggg caaacgccga
    37021 ttgtttctgg agaaatcgct gaagatttta cttactatct tgctatctca gaacaaatcc
    37081 cttcagctgt tggactgagt gtattggtca atactgacga cagcatcaag acggcgggag
    37141 gattcatgat ccaaatcatg ccaggtgccg atgaagaaac aatcagcaag atcgaagaac
    37201 aattaaaaac gacaccaaga atctctaccc tcttagatga aggacagaca ccagaagaaa
    37261 tattgaaagg attgcttggt acagaagaat tgaacatttt ggaaaaaatg cctgttcaat
    37321 tcaaatgtga ctgttcaaaa gaaaaatttg cagaagcaat tatcgcgtta ggtgcagaag
    37381 aaatccaagc aatgatagac gaagatcacg gtgcagaagc ggtttgttct ttctgtaata
    37441 acaaatacaa gtatagcgaa gaagaattag atggattgaa gaaagaagcc ttaggaggga
    37501 tttaagtgga acacacatgg aaaattggca atgtggaaat ccctaatcgt gtcgttgttg
    37561 caccgatggc tggaataaca aattcagcat ttcgagtcac tgtcaaagaa tttggtgcgg
    37621 gcttagtcgt atgtgaaatg atcagtgacc aaggtattca tttcagaaac aaaaaaactt
    37681 tagagatgct ccacattgaa gaaacagaac atccattaag tttacagata tttggcggaa
    37741 ataaagatag tctagttgaa gctgctcaat ttgtcgaaga aaacacagct gccgatataa
    37801 tagatatcaa tatgggctgt ccagtaaata aagtaatcaa agcagaagca ggagcaaaat
    37861 ggcttttaga tcctgagaaa gtatacgaaa tggtccaagc tgtttcttca gcagtaaata
    37921 tcccagtaac aatcaagatg cgtattggtt gggatgaaga gcacgtcttt gctgtagaaa
    37981 atgcattagc agcacaatca gctggagcaa gtgccatcgc tatgcatgga cgtacacggg
    38041 ttcagatgta tgaaggaaaa gcggactggg aagtattgaa agaagtgaaa aaacatttga
    38101 cgattccatt tatggggaac ggggatgtca aaacgccaga agatgcaaaa tggatgcttg
    38161 actatgtcgg tgcagacggt gtgatgattg gtcgtgcagc gctgggcaat ccatggatga
    38221 ttcaccgcac acagcattat ctcgaaacag gggaactgat tcctgaacca acaccagccg
    38281 aaaaaattgc tacagctaaa cttcatttac aacgcttagc agatctcaaa ggagaaaaag
    38341 tagcttgtag agaattcaga acacatgcgg cttattattt aaaagggatt cctcgagcag
    38401 cgaaagtgaa agtagcaatc aatcagacag aaaaacagca agaaatcaat gacctgctgg
    38461 atcaatttgt agaaaaagcg gaaaaagtac tattgaaaca aacgttgtga tttctaagtt
    38521 tttcgtgaat tttcaagccg aaattccaag ttcggcttgc tttttttgtg tttcattggc
    38581 attataggaa agagattaac atgagaatgg aggaactttt gtgacacagg aacaacaaca
    38641 tcaagtagaa gatttaaatg atcaaatgct tgtacgtcgt caaaaaatgg aacaattgcg
    38701 tgaagatgga atcgatccat ttggaaaacg atttgaacgc acgcacaact cagaagaatt
    38761 gcatgaacta tttgatccaa gaacaaaaga agaactagca gaaatgggat tgaccgctag
    38821 tgtagctggt cgtatgatga ctcgccgagg aaaagggaaa gctggatttg cgcatctaca
    38881 agatcgtgaa ggccaaattc aaatttatgt aagaaaagac caagtaggtg atgaggccta
    38941 tgaagtattc aaacatgcag accttggaga tttctttggt gtaactggac aaatcatgaa
    39001 aacagacaca ggcgaagttt ctatcaaagc ttcagaaatc acgattttgt caaaagcact
    39061 tcgtccactg ccagataaat accatggctt aacgaatatt gaacaacgct acagacaacg
    39121 ctacttggat ttaatcagta atcgcgaaag ctttgaccga tttatgaaac gaagccaaat
    39181 tatcagcgaa atccgtcgct atttagatgg caatggctat attgaagtag aaacaccagt
    39241 attgcacaat gaggcaggtg gagctgccgc acgcccattt atcacgcatc ataatgcact
    39301 ggacattgat ttatatttgc gtattgcgct agaacttcat ttaaaacgac taattgttgg
    39361 cggaatggaa aaagtttatg aaatcggccg cgttttccgt aatgaaggaa ttgatactac
    39421 acataatcca gaatttacga tgttagaagc atacacagca tatactgatt tcaatgatgt
    39481 aatgaatctg acagaaggta ttatccgtaa cgcagctgaa aaagtactag gaacagcaaa
    39541 aatcacatat gatggacaag ctgtcgactt agaaagtgac tttaaacgca tccatatggt
    39601 agatgcaatc aaagaacaaa ctggtgtaga cttctggcaa gaaatgacat tggaagaagc
    39661 attagcttta gccgaaaaac acaatgtaga agtaaccgaa gctatgggtg tcggacatgt
    39721 tatcaatgaa ttctttgaaa catttgttga agaaacattg acacaaccga cgtttgtata
    39781 cggacatcca gtcgaagttt cgcctttagc gaagaaaaat ccagaagatc ctcgattcac
    39841 agatcgattc gaactattca tagtggggcg cgaatttgct aatgccttta cagaactgaa
    39901 cgatccaatt gatcaaagag aaagattcga agcgcaagaa aaagaacgtg aactaggaaa
    39961 tgacgaagct cacggtgtag acgaagactt cttggaagcc ttagaatacg gtatgccgcc
    40021 aactggtggt ctaggcatcg ggatcgacag attagtaatg ttgctaactg atgcacaatc
    40081 aattcgggat gttttgctat tcccaacaat gagataatcg aataaaagct gtctaaggaa
    40141 ttccttaggc agcttttgct gtgtagaaag gaataaataa taacgaaagt tcaaaagcta
    40201 atgccgacta ttctagacat taaagaagac gaaaacgaac gataaagaaa gaaaagttat
    40261 gaataatcgt ttattgaaaa aaacttgaaa aaatacaaaa aattcgttga ccaagctaaa
    40321 tttagttggt atattattac ttgtccaaaa gacagaagaa aatatttatt caaaaacata
    40381 acaaaaaagt tattgacatg ttactaacga catgataaga taactgagtc gttaaaaaag
    40441 aaccacttgc gaagcttgat tctatcgaaa aaaacttcaa aaaagttctt gacaaataac
    40501 aaacgataag ttatgataaa tgagttgccg aaagatttcg aaaaacgaaa tcgaaaaaaa
    40561 cttttaaaaa agagttgaca atcgcttgtc tctctgatat gatataaaag ttgctacggc
    40621 agccaaaaat gaatcgggaa acactctcga tgaa
//
LOCUS       DACCXO010000016        37767 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.016, whole genome shotgun sequence.
ACCESSION   DACCXO010000016 DACCXO010000000
VERSION     DACCXO010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.016"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            28..909
                     /locus_tag="IVY70_000765"
     CDS             28..909
                     /locus_tag="IVY70_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7957917.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            922..2532
                     /locus_tag="IVY70_000766"
     CDS             922..2532
                     /locus_tag="IVY70_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP7957918.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2609..3268)
                     /locus_tag="IVY70_000767"
     CDS             complement(2609..3268)
                     /locus_tag="IVY70_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP7957919.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3551..4216)
                     /locus_tag="IVY70_000768"
     CDS             complement(3551..4216)
                     /locus_tag="IVY70_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957920.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4363..4968)
                     /gene="dhaL"
                     /locus_tag="IVY70_000769"
     CDS             complement(4363..4968)
                     /gene="dhaL"
                     /locus_tag="IVY70_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP7957921.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(4996..5979)
                     /gene="dhaK"
                     /locus_tag="IVY70_000770"
     CDS             complement(4996..5979)
                     /gene="dhaK"
                     /locus_tag="IVY70_000770"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP7957922.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(5981..6376)
                     /gene="dhaM"
                     /locus_tag="IVY70_000771"
     CDS             complement(5981..6376)
                     /gene="dhaM"
                     /locus_tag="IVY70_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP7957923.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6530..6850
                     /locus_tag="IVY70_000772"
     CDS             6530..6850
                     /locus_tag="IVY70_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP7957924.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6962..7831)
                     /locus_tag="IVY70_000773"
     CDS             complement(6962..7831)
                     /locus_tag="IVY70_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7957925.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(7927..9231)
                     /locus_tag="IVY70_000774"
     CDS             complement(7927..9231)
                     /locus_tag="IVY70_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7957926.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9228..9737)
                     /locus_tag="IVY70_000775"
     CDS             complement(9228..9737)
                     /locus_tag="IVY70_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957927.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9912..12047)
                     /locus_tag="IVY70_000776"
     CDS             complement(9912..12047)
                     /locus_tag="IVY70_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7957928.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12130..13785)
                     /locus_tag="IVY70_000777"
     CDS             complement(12130..13785)
                     /locus_tag="IVY70_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7957929.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13787..14101)
                     /locus_tag="IVY70_000778"
     CDS             complement(13787..14101)
                     /locus_tag="IVY70_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP7957930.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14271..14531
                     /locus_tag="IVY70_000779"
     CDS             14271..14531
                     /locus_tag="IVY70_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP7957931.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14545..14859
                     /locus_tag="IVY70_000780"
     CDS             14545..14859
                     /locus_tag="IVY70_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP7957932.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15589..16143)
                     /locus_tag="IVY70_000781"
     CDS             complement(15589..16143)
                     /locus_tag="IVY70_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP7957933.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16269..18198)
                     /locus_tag="IVY70_000782"
                     /pseudo
     CDS             complement(16269..18198)
                     /locus_tag="IVY70_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18504..19220)
                     /locus_tag="IVY70_000783"
     CDS             complement(18504..19220)
                     /locus_tag="IVY70_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP7957934.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19221..21044)
                     /gene="glpO"
                     /locus_tag="IVY70_000784"
     CDS             complement(19221..21044)
                     /gene="glpO"
                     /locus_tag="IVY70_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP7957935.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21058..22554)
                     /gene="glpK"
                     /locus_tag="IVY70_000785"
     CDS             complement(21058..22554)
                     /gene="glpK"
                     /locus_tag="IVY70_000785"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP7957936.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22690..24150)
                     /locus_tag="IVY70_000786"
     CDS             complement(22690..24150)
                     /locus_tag="IVY70_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7957937.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24140..25435)
                     /locus_tag="IVY70_000787"
     CDS             complement(24140..25435)
                     /locus_tag="IVY70_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7957938.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25767..26444)
                     /locus_tag="IVY70_000788"
     CDS             complement(25767..26444)
                     /locus_tag="IVY70_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP7957939.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26474..27565)
                     /locus_tag="IVY70_000789"
     CDS             complement(26474..27565)
                     /locus_tag="IVY70_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP7957940.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(27666..28100)
                     /locus_tag="IVY70_000790"
     CDS             complement(27666..28100)
                     /locus_tag="IVY70_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7957941.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28684..30141)
                     /gene="ascB"
                     /locus_tag="IVY70_000791"
     CDS             complement(28684..30141)
                     /gene="ascB"
                     /locus_tag="IVY70_000791"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP7957942.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30138..32033)
                     /locus_tag="IVY70_000792"
     CDS             complement(30138..32033)
                     /locus_tag="IVY70_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7957943.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32091..33866)
                     /locus_tag="IVY70_000793"
     CDS             complement(32091..33866)
                     /locus_tag="IVY70_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7957944.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33916..35412)
                     /locus_tag="IVY70_000794"
     CDS             complement(33916..35412)
                     /locus_tag="IVY70_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7957945.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35606..37228)
                     /locus_tag="IVY70_000795"
     CDS             complement(35606..37228)
                     /locus_tag="IVY70_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP7957946.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            37636..>37767
                     /locus_tag="IVY70_000796"
     CDS             37636..>37767
                     /locus_tag="IVY70_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP7957947.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT        10551 a         8053 c         6515 g        12648 t
ORIGIN      
        1 atttttagaa aaaggagtgg ggtttttatg gcagaaatcg tcaaagtcac acatttgaaa
       61 aagagctttg gtcgtttcga agctttaaaa gatgtctctt tcaccgtaca ttcaggtgaa
      121 gtcgttggat ttatcggacc aaatggttct ggaaaatcca cgacgattcg gacactttta
      181 gggattataa aaaaagacgg cggaacggca acgatttttg ggcaagatgt atgggaagat
      241 agtttatcta tccatgaacg tctttcttat gttccaggcg atatctcttt gtggggaaat
      301 ttgactggag gagcgatcat cgatctcttc atgaagcttc atggaaaagg gagtgctcaa
      361 aaaaaagatg aactgattca acgtttctct cttgatccta agaaaaaagc aaaaaactat
      421 tcaaaaggaa atcgccaaaa agtgggcttg attgctgctt tggctgttga ttcggatctc
      481 tatttatttg atgagcctac ttctggttta gatcctttga tggaaacgat tttccaagaa
      541 gaagttgaaa aattgaaaga acagggcaaa tccatcctac tttcttctca tattctcagt
      601 gaagtcgagc gactagcgga taaagtcgtg atcatacgcg aaggaacact cgatgaagaa
      661 ggaacactcg atgaattgcg tcatttgact cgttcgacca tcactataga aacagaagca
      721 gagaatagtc acttagcaca attgaacggt gtccatgact ttgtgcaaaa aggtcacctc
      781 gctcaatttg ctgctgataa cgaagctttc gatgtgattt tagctgaagc gtccaaatta
      841 ggtgtgaaaa aatttgaatc tattccaccg actcttgagg atttattcat gagacattat
      901 gaaggatgag gaggaagcga aatgactgat aaattttcac gctggtccac ccttttttct
      961 caaaacctta gccgtgactg gaaaaagatc ctgatatggc tagtcggtgt cactgctttt
     1021 tctggtggtt ttgtccctgc gtttgaagaa ttgacttccg gacaaggatt agctggatta
     1081 tacgaaacct tgcaaaaccc ggcaatgatt gcaatggtcg gaccaactcc agcttcgaat
     1141 gctgctgagt atactttagg agcgatgtat gcaaatgaaa tgttgctttt ttgcagtttg
     1201 tttgctgctg tgatctctgt tttacacgtg gtgagccaaa cgagaaaaaa agaagaactt
     1261 ggtctgattg aacttattcg ttcctttcga gtcggacgtc aagcaaactc tttagctgtc
     1321 atcgctgaaa gtattctgat caatttgctt ttagcaatat cgatcagctg tacgatgatt
     1381 tttttccaag cggattcgat cactgcagaa ggtgcttggc tttttgggct atctgtaggt
     1441 agtatgggga ttttggggag tgccgttgca ctgtttgctg cacaactgat gccaagttca
     1501 gccagttcga atggtctgtc tttagcgttt ttaggtggac tttaccttct acgagcaata
     1561 acagatgtag ctgattcctc cctttcgatg tggaatcctt taggctggtc ttatttgacc
     1621 tatccattta caactaacaa ctggattcct ttgctttatt tgtttttgtt cagtctaatc
     1681 ttgataatca gttcatttgt tttagaggaa catcgagatt tgaacaccgg ctatatcccc
     1741 gaacgtgaag gaaaaccgta tgctaaacgt tctcttttat ccttgcgtgg tttattattc
     1801 tatctaaata aagggatgac aatcagttgg cttatcactt ttggtttgat gggggcggca
     1861 tacggctcta tctacgggaa tatgcaacag tttttagaaa gtaacgactt aatgaaacag
     1921 atgtttatcc aatctggcgt ctcaattgaa aaaaacttta caagcacgat catggtagtc
     1981 ttgatttgtc tagctgctat tttaccaatt gctagcatca atcgtttatt tacagaagaa
     2041 acccacggac gtatgaatca gctgttcgca acaaaaattt ctaggaacca actttattgg
     2101 accaatatgt tactagctat tctatttgct atattggcaa tcctgattag ttctggtgca
     2161 ctagggcttt ctgctttggc ttcgatgaag gatgcgaaag acatggatct attgatgttt
     2221 cttaaagccg ggttcaattt cctgcctgcc gttttagtgt ttatcggaat tagtggcttt
     2281 ctattcggat ggcttcctcg aatcggtaaa cttgcttata tctacttagc ttactccttt
     2341 gcactcaatt actttggcgg aattttggat ttaccagaat ggttttccaa aacagctgtc
     2401 cctagctggg ttccacggat gccaaacgat tcttttgatc tttccgtgtt ccttacgtta
     2461 tcatgtatca gccttgcact gttcgtcctt ggttctattg gttataggcg aagagactta
     2521 ctagaaggat aataaaaaag catgtacaat ttcatgctag cctccaaatc tgggtcaaaa
     2581 atccaacatt tggaggtcat ttttttattt acaagtcttc taactgctct ttatcaatga
     2641 tccagacttc tcgattcttt ctttttacat aaccattctc ctctaataat ttaaattttc
     2701 tagataaagt ttcgggggtc gtaccgataa atgcagcaag ttctttcatt ttcattggca
     2761 ggaaaaagtg atccttccct gtcgctttag ctaagtcaag cagatagaca gcgactcgtt
     2821 cttctacttt ttccatcatc aaaaaagttg tctgctgctg tacttttgtt aatttctcaa
     2881 cgttgattgc taaaagtttc agactaaggg cgggataatg cagtaagagt tccgagaaat
     2941 ctttttgtct caatatgcaa atttccgttt tttctagtgc ttcgccaaat aaagactcat
     3001 ttggaacgcc tagcaactgg ctctctccct catatccgcc cggctctatt actcgtaaaa
     3061 gctgttcctt tccgctagca gataattgat acactttcat actgccggag gccacgatca
     3121 ctaattgagg ttctccattc ggcgcaagaa tctgttctcc tttttccaca tagtgatgtt
     3181 tgacaagtgc gtgtactttt tcttgatctt gctgtactaa gtgattgaac aaaggaacaa
     3241 atgaaacaca taaatgatca tgactcataa tctctcctcc ttctaaacag ctatttctaa
     3301 taaagaaaca taacttccct tattccaact gataattttc ttttatcata gcataaacgt
     3361 caagcgaacc agataccagc aaatagagtg gatttctttt tgtcccttgc tttattttcc
     3421 ttcttttgag accttaagta attgattcct atcaattttt taaatattta ctttttgaat
     3481 aatcaaaaac aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg cattgcttat
     3541 ttcgtgttgt ctattgaaat ttttccgcac ctagcttttg atatagtggt gctagttctt
     3601 gtaaggtttc agcggttatt tgctcaacgt gttcgatttc ttgcggatag aagaatagtt
     3661 tacccatttg aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt ttctttgtat
     3721 cggacaaata gtcagacaaa gctaagtggt cgtcaagttc atgttctggt tgcggccata
     3781 tgtaataatc cttgatatcg actgttttgc tccactcttt cagtatcgca agcaactggt
     3841 tatacgctgc aatgctacca tatcgttctt cgcttttata atgttggtac atcaaataga
     3901 tttggtattg tttttgattc aacaaaactg ctagacaggc attctcgtct tgacgagatt
     3961 cgcttcggta cgcgctccaa aaatgactgc gtaaattcca gccattcgtc cagctttcga
     4021 ttttaggttt gctcatgccg tacatatcag gcaaaagtgc agctgtctgg agctgaatct
     4081 ctttccattt ttgccagtgt tctttatatt ctttcttgat ttgttcaatc ttatctgttg
     4141 gatggctctc tttgagctgt ttgaatgcgt agattggtcg atcaaacaac tgataatctg
     4201 attttccaag ttccattgtt tcgcctcttt ccgttatctt ttgtattcta cctgaatttt
     4261 gcgatatgga caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat aagaacagcc
     4321 aaacaaaaaa ctgcgatgag catcaccgca gttttattga acttaaggtt gattgatcac
     4381 atctaaaagc gttgaaaata aaatggcact agaagcagct cctgggtcga tatgtccgat
     4441 tgcacgctct cctagatagg aagctcgtcc ttttttagct accaagactt tggtgtgttc
     4501 ttttgcctgg tcaatgattt cttttgttaa attccctgcc ttcaatgcct ctgtgactgg
     4561 cagccaaaca tctaacatcg ttttatcgtc agcttctgcc tttcctcggc tttgaatacc
     4621 cgctgttccc tgctcgatca cttcgcctaa tttttcttga gaatcaatag aatcgacctc
     4681 ttttgtcgct ttcatcatgt tcatgaatgc agatccgtat aacggaccag aagccccacc
     4741 gactttcgat atcatcgcca tagagaatac tttgaatgta tcgctgattg tttcaggatg
     4801 atcggcttga aaagcttctt cataagctga aacccctctt cccatgttat ttccatggtc
     4861 tccatcgcca atcggggtat ctagatcact gagatattgt ttgttctgat tgattgcctc
     4921 tgcaaattgc gtgagccatt cttggatttc ttgaacggtt agtttcatat tatcaccctt
     4981 ttatcttgag attttttacc atgaaatcgt ctgtacattg ctttctaaag aatctttcca
     5041 ttgtgtctcc gtcaagtcga tcagtgttag agaaagacct tgcatatcta gtgaggtcat
     5101 aaaattaccg acttttcgga aaactacatt gacgcctctt tcttccaaca gagctaaaac
     5161 atcattcatg aacacgaact gttccatcaa aggcgtcccg cccattccgt tgactagtac
     5221 acctgcttct tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa gttcctttgc
     5281 cagtacttta gaaggctgca tcttttctcg acgatagcct ggctcaccgt ggataccaac
     5341 accatactcg atctcatctt ctcctagttc gaatcctggt ttccctactt ctggtacagt
     5401 tgctgctcga agcgctagac cgatcgtctt agttgcctgt acgacctttt ctcccagttc
     5461 tttcaactca gctagggaag caccgtttct tgctgcatcg cccacgattt tgtgtacaag
     5521 gacagtacct gcaactccgc gttttccagc agtatacgta ctattttcaa cggcgatatc
     5581 atcatctacg atcacacttt ctacttcaat atcgtccata gcagctaatt cctttgccat
     5641 atcgaagttc aatgcatccc ctgtataatt cttaacgatc agcaaaactc cttttccact
     5701 atcggcttcc ttaatagccg tctgaatctg atctggagtt ggagaagtaa acacatcacc
     5761 aagcaccgct gcacttaaca tcccttcccc tacaaacccg gcatgggatg gttcatgtcc
     5821 gcttcctcca ccagaaacca aaccaacttg ttccgctttc tgattcttgg ctacaacacg
     5881 ggaagattct acacgatgaa caagttctgg atagcttttt actaatcctt ccagcatttc
     5941 ttcgacaata ttgcttggat cattcatgat ttttttcaat ttattcccat cctttctttc
     6001 cggcattttg tgcttctgtt aagatttgct gagtatcatc tgttataccg gcagtgatgg
     6061 ctgctgcaaa agctccttcc actaaaggcg catccaccag atgataacgt tgctgctgct
     6121 cttcttccaa catgtctttt gccagttccg agttcaatac cgcgctaccg atatccgcaa
     6181 aaacaaaata actagctgca tcgcttgatt cattgactgc ttctacgatt ttcatcgggt
     6241 ctgatccgat ctcgccattt attccgccga gagaaaaaat ttgcacctct tcggattttt
     6301 gcatctgctc aatcatttct ttaataccat ctgttacttt ttgactatga gaaataagca
     6361 aaatactttt tttcacaaga atcttccttt cttttacata gttgctaacg attggtttcg
     6421 ctttcattaa tgaacaattc aagtatataa taaatttcta attttgggaa aatatgtgta
     6481 gtgcttcttg aagtttgtta tactttaatt aaaagagagg agtgatcgaa tgattacaac
     6541 aacaacgaac agtgtagagg gaaaagccgt agtagcttat aaagggatcg tttttggtga
     6601 ggtcattact gggatccatg catttaaaga cttggaggcg gggttgcgaa atatctttgg
     6661 cggccgctca aaaagctatg aaaacgaact aatgggtgca agagaagaag cacttgccga
     6721 aatggccgaa cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa aaatggacta
     6781 tgaagtatta ggcagcgaca atggcatgct gatggtcaca tgtagtggaa cagctgtctc
     6841 cacgagataa gtaggggaca tctgttttaa taactaaaga gagatgggac atgtagtggc
     6901 actcaagaat tagatttttc agtctaactt cgaggtactc taccttccca tcctctcttt
     6961 tttatttctt tttgcggatg attccgtaag ctgctgcgac taacacaccc gcccctgcta
     7021 aggaataact gacagaagct gttttttcat ttgtttttgg caaagtctga cttgttgatt
     7081 tggatggctc tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc actgattttt
     7141 ctcctgccga atgcaaaata tcttttattc gttggaaact agctgctaag tcagcttttt
     7201 ttgcttcaaa atcattagca actgtttgtg cttcgctgat ctttccgtgg attgcttgac
     7261 tggccgctgt gatatccgct tgagtttgac ttagctttcc agaagctgtt tgagcaacct
     7321 gatttaattg ctgattgaat tcttttgctt gttggatctt cccgtcaaga tctgtttgga
     7381 cagcttccgc ttttgctttc gcttcggcaa gctttttctc aagttgtgca cggtcatttc
     7441 ccactttatt tttaagatca gctaaatctt gatcgattgc tgcttggtct tctttcaatt
     7501 tgttttttgc agcttctaat gcttcttcga gttcttttct tttattttcc aagtcttgca
     7561 aatgatcgat ttggtcagaa gaggtagaac tatttattga tgtatcttct gaagatgaaa
     7621 tcgtttctcc tgaattatct gtcggtacat tggaagtcat ttctgtttcc gaggcaccgt
     7681 tttcagcacc agaagagctt atggttgttg aaggttctgt ttgagcaggt tcagaagttt
     7741 ccgcaaaagc tgtctgtccc aataagaaaa tactggaaac agcaattggc cagattaacg
     7801 gcttcattct ttttttcatc acttttccca tccttactac tttttaacta atttcttatt
     7861 ataagcaaaa atgtttggaa gaaccataaa cacaacaggc tgtttgtcta aaaaacggtg
     7921 catcttttat ttttttgtac gttccgggct tttccatgtc ccgcttccat aacctaaaat
     7981 cgttttgact gctggaatga aagtcgtcac gatcgttatc gcgttcacta tccagaacaa
     8041 aagcatatat agtggtgcga aaagaaggta tttcaatttt cttcgtcgat catctacaat
     8101 cagcgaagct agcaactgga aaaatcctgc gaccatttca aagcagatga acacaaaact
     8161 gaatgtaaaa gtaatataaa tctgttcgta gtttccttga tatacataat gcccgattaa
     8221 tccagcaaaa agaactacgg aaatacaaaa gaatattgac cagacaatac tcaatgtttg
     8281 atcaatgaag atgatcgttc ttccaatatg tttaaaagga tggagcatca cttttttgaa
     8341 attcgtcagc cagacttctg ttccaccttt tgcccaacgt tttctgtgtc gatatagcat
     8401 cttcaacgtc acaggtactt ccatgaaaaa gataatctca ggagcaaaaa cagataacca
     8461 gtcatttaac tgatgatccc aagcaatact gatgtcttct gtcgctcgat cttgtctgaa
     8521 tccgcccaca tcgatcaagg cttcttttcg atacatcgta tttgctccac tgtacgcata
     8581 aagaccaccg aaaacagctg actgtgttcg tttaatgatg cccacgatac ttgagaattc
     8641 aaccgtttga gattttgcaa tcaatttggt acgattttgg acatccatat ttgcagtgac
     8701 cgcagcaata tgtcttgcac cagggcgaat gaaataattc acatacctaa tcagtgcatc
     8761 tggctccggt accgtgtctg catcattgct taaaatcaat ttcccttttg caaaggcaag
     8821 tccgatatta aacgcgtggg cttttccttt atttttttcg atccgaacca cacgaagatt
     8881 agcgtatttt ttcatcagtc tagctaatat ttctggtgtt tggtccgttg aaccatcgtc
     8941 tgtcactaat acctcatagt tgtgataatt caatttcgtc atcaaatact cgattgtata
     9001 ttcgatcaca atttcttcat tatgggctgg caccatgatc gtgatgaacg gttcgacact
     9061 caagggaaca tctacccatt ctttttgctt atgtttgtat aaaaacacat aacaccagac
     9121 accaataaac catacaaatc cgcctaaaat cggataagaa acgagaaaaa aatacataat
     9181 agacaaacaa gtatctaaga ataaattacc tgtatgggtg attgagatca tttcagttcc
     9241 accccatttt ctttatacaa atctgcaatg aatgtcgtat ctagattttt ctcttcggag
     9301 acagaataaa aacggaccgt ttctctctct tcttttgttc caaaacgttc cgtatacact
     9361 tcttcaagta attcttgtct ttttttcaat ttttcatctt catattcaaa agatttattt
     9421 acttttttgt atagatttcg attgttccac ctagtcaata caaatgaggt aatcaggaaa
     9481 ctgaaaaaaa tcaatacaaa aaagccgatt aagaaataca gcattttctt ttctgataaa
     9541 aaagcgtaca aggaaaaatc cttggctctt tctggtctcg ttaatgggaa aaagacccat
     9601 ataaaaggaa gaacaacgat catccatcct aaaagagcca ctaacgtttg ccaaatcttt
     9661 aaccagtaat taccttttga aaaatactta tctttcacca aaattgatgc aacttcttct
     9721 cttgttactt ttttcatatc ctcacctcac gtttttttgt tacgacgtag gagtttacca
     9781 tacatttcat ttcttttcac ataaagtgtt cctaaatttt tcctaagaaa aagaacaaaa
     9841 catttctcat cagcaaatga aaaatatttt gttcttttta tcagttgttc tttttatttt
     9901 ttatctctta attatgttgc tctttccaat acgacaatgc ttttcattcc tctagatatc
     9961 gccgtgtaca tttgtctgcg tcttctttgc tcatctccat cgttttcaaa tggatataag
    10021 attacatgat caaattccag tccttttgct agactcaaag gaatgatttg gcagttttct
    10081 aatccatatg aatttttttt aatcgattct tgtagcgttt gggtttcctc ttctgtttgg
    10141 gtgataactg ccgctttttc tccttttaga gaaaccaaaa tctcagctag tattttcaac
    10201 aattcttcag aggatcgaaa agagcggtat tcgggttctt ttccttgcgg tcggatcgat
    10261 acgatgtcga tcttctcttg atccacagca taggttttga aaagctctgt gattgctccg
    10321 ctagaacggt aactatttct aagatcatac cgatggatcg gcagatggta gtcgtcgaaa
    10381 caacgcataa tatcagcaaa agtagtggaa gaattaaaga ttgcctgatt ctcgtcacct
    10441 actaaagtaa agtgagtttt tggaaataat tcgatcaata gacctaattg tgccagggta
    10501 taatcttgta cttcatcaat caatagatag cgaaatgctg gtacatttac cttttcaaca
    10561 agtaaatgac gaatagctaa tataatgact gcttcatcaa cagtacgtgt gggttgccaa
    10621 gcataagctg agccatatcg tttttcataa attctttcaa acaactgatg ctcatccacc
    10681 catgccattt cttcgatttg tctggttatt tttcgatatt ttttttgtaa aagtttcctt
    10741 gcataagcta caatactttg ttccgaatca tcagaaatca atttaccgaa taattccaac
    10801 tgcctatctt ctgtcaacga gagtacctga ttctgcacag caggactttt tgcttgtttc
    10861 aaaagatgat tcttccattc gctcatcagt gcctttttcg ttgcttgaag cctatcgatc
    10921 aatttaggat gagcaggaac tttctcatat attttttcga tcgttttttt agaaaggact
    10981 gtttttccat ttttagtaag tccacggata aaattcggat gatctaaaaa caaaggatct
    11041 gattgtttta gattatcgat aaactttttg ctacgcaaac gttctgtctg ctccgataca
    11101 ttttctcccg tgatacgttt gaaataagcc tcttcacctt ctatttcctc tgctgaaagc
    11161 tgtgaaacca gctgtatgat ggttagattc agtggatttc tttcacctaa tgatggtagg
    11221 acattcgaaa tatagtcgat aaaccgatga ttaggtgata gaatcagcaa gtcatcggat
    11281 gtcatcgtat ctcgatataa atagagcaga tacgcaatac gctgcatgat agcagaggtt
    11341 ttaccacttc ctgctacgcc atttaccaag atattttgac ttttcacatc ccgaatgatt
    11401 gtgttttgtt ccgattggat cgtagcagtg atagccttca tttcattggt ctcatcttgg
    11461 cctaagactt ctaataatac gtcatcttga atagctacag acgtatcaaa aaacttgacc
    11521 aaacgatctc tttccaaaag aaattgtcgt ctttctttaa taaccgtatc gattttattt
    11581 tgacgaaccg tataagaaga tggaccaatc atgttattgt aaaaaagttc ggcaattggt
    11641 gaacgccaat cataaacaag attttcatct gcatcatctg tgaaatttgc tgtaccgata
    11701 taaaaggatt cttttggctc attatccaga aagtctacaa cgacttttcc aaagtaggga
    11761 cttttcaata aacgttcgat ctttgctagc tgattttctg cgtttttctg tttgatattc
    11821 atctgatcga tttctctatt tttcatttca atcatcgaaa aagtatccag gttatctaag
    11881 taactgtcaa aattcaatcg aagctcgcca gccatttctt tcaacgactg gtttccttct
    11941 atttttgccg tatccatcga ttcccgcagt tcgttttttt tatcaattag cttttgatac
    12001 acgttcttca gatatgcttc ttctaactgt ttttgtgtgt tgaccataca cattctcctc
    12061 tttttttaaa gtggtggaac cgattagttt acctctttta agagtcgaat gcaagctttt
    12121 taacgagtat catttgttag gatatatcag ttcttcaggt cggacagttt gatataaaga
    12181 aaatgcacca tcaaggtttt ttacgtggta gccagactgc ttcaagattc tttccgctag
    12241 atagctacgt aatccgctat gacagctgac gatatattct tgttggctgt ctaattcatt
    12301 taatcggtcc cgaagttcat tcaaagggat atgtttggct tccttgaatt tgcccttttc
    12361 caattcttct gctgttcgca catctaataa aatttttcct tttgctagtt ctgtcggcaa
    12421 ttcatgccat tgtacggttt cactaagccc ttctgcaatg ttcattgctg catatcctag
    12481 catgtttact ggatctttgg ctgagccaaa tggcggtgca tacgtcaatt ctaattccgg
    12541 taaatcgaag atcgtcaaat gccctttgat tgctgttgct aaaatatcga tccgtttatc
    12601 cacacctttt gccccaacac cttgtgcacc ataaatctcg ccagtctctg gatggaagat
    12661 caacttcagt aaaatatctg ttgcttctgg ataatagctt gcatgatctt ttcctgatac
    12721 atgaatgact tcaaaagaca gttcgttttc tttggctgtt ctttcactta aaccggtgga
    12781 tgcggcagtc attccaaacg ttcgtataat tgctgttcca ataccgcctt gatttcttcg
    12841 agaaattccg gaaatcgtgt ccgcaacttg tctaccttga cgattggctg gtgaagcaag
    12901 agaaataagt gcatcttttc cagtgatttg ctgttttaca acgatagcat cccccaccgc
    12961 ataaatatca ggaatattcg tttgataacg ttcgtctact aggatacccc cgcgtatgcc
    13021 taattcgatt ccagcggtca ctgctagctg gttatcagga cgaacaccaa tggataaaat
    13081 aactaaatca gcagggatgg tacgcccatc atccagtcgc aatcgtttcc cgtggtcttc
    13141 aaatccagcg acagcatgag aagtaatcac ttctactcct tgatgagaca attctttttc
    13201 tataaaagct gccatttctt catccagtgt tggaagaata tgtggtgctt gttcgatcac
    13261 catgacttct aaacctcttc tttttaggtt ttctgccatt tccaatccga tgaatcctgc
    13321 accaacgatc acggcacgct ttggctgttt ttctaatgca tgcatcactt tatcgatatc
    13381 tggtacattt ctaatagaaa aaacattgtc ggcttctgtt atccctggaa tcgatggaac
    13441 aactggtttt gcaccaggag ataaaaccaa tgcatcgtac tgttcaatgt gtttttgacc
    13501 ctcatgtacg acttctaccg ttttgttttc gggaaagatg gctgtcactt catgatttgg
    13561 acgcacatct aaacaaaaac gagcggataa tgattctggc gtttggacaa gaagattttc
    13621 ccgttcactg atttctcccg aaagataata aggcaagccg caatttgcaa aagaaacata
    13681 cggtcctttt tcaaaaacaa cgatttctgc atcttccatt aatcggcgaa gccgtgtagc
    13741 agcagacatt ccacctgcta cacctccgac aataacgatt ttcatcttat ttccctccta
    13801 ctgttcgatc aaaccattga ttcatgccac cacgtacatt cactacatca taaccagatt
    13861 tctttaattc tttagctgct tgtttactcc tcatccctga ttgacaaatc acatagacgg
    13921 tcttttcttt gtctccatca tatctgttta tactttgtaa tggaacattt ttagctcctt
    13981 ttatatgccc gccccggtat tctgcaggtg ttctaacatc aagtaacgct ggattttcca
    14041 aaagcttttc ctttaattct gggactgtta tcgattttat tgattgaaat agcaaattca
    14101 ttttctgcac ctcatttaca tttttataaa tacctcatgg ggtatattaa acaggagctt
    14161 tattaatatg tcaaccagaa tgactcttca tttttttcac tactttttat atttgcataa
    14221 aataccgata aggggtatta tctaattata aaaatagagg agaatgtgtt atggagattg
    14281 ataataaaaa tatcctgaac cgtttgaaac gtacagaggg acaaattcgt ggtatccaaa
    14341 aaatgatcga agacgaaaaa gaatgtatgg atgtcatcac tcaattaagc gctgttcgtt
    14401 ctagtattga tcgcgtaatg ggcatgatcg ttgcagataa tctgaaaaat tgctttgaga
    14461 aacctgatag taatccagaa gaacaagcgg ctaaactaga acaagcgatc aagatgatta
    14521 ttaaaaagta aaggaatgat ttcaatgaaa gtcgttttcc atattgatga attagaaaaa
    14581 tggtcagaaa cagggaaaaa cgtaaaaaac ctgataaaag cttctccaga aacgacgatt
    14641 gtcgtatctg taaatggcat cgcgattacc ggttatctag actctgccaa tgcagaattc
    14701 ttagatttgc aaggagtcgt ttttcacgct tgcgccaatg ctatgcgtgc taatcatatt
    14761 tcagaaagtt ctttacctga acaagtaatc gttgtaccag caggtgtcct tgatttagtt
    14821 gaactgcaat cccaaggtta tgcttatatc aagccttagt cagtagaggt tgcgacagaa
    14881 gtgatcagct tcaagaaatt tcggcttatt tttcgaagaa gctacttctg gaaaaccgtg
    14941 aatcaaaggt tgtgaaaacg ctgtcctgca tcaagtaata agaacagcca aacaaaaata
    15001 gcttctcata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
    15061 tttaaggaag aggttgtgaa atcagcggtt tgacctaagt attagagaaa aaaacgggaa
    15121 aatagctcct catattttgc cgttttttga gctaataccg aaggtcagct gattgaacac
    15181 cgtttaagga agagattgtg aaaaagtcgc ttagtttcga gtattaagaa aaattttgtg
    15241 aaaatggatt tctacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa
    15301 taccgtttaa ggaagaggtt gtaaaatcag cggtttgacc tgagtattag agaaacaaac
    15361 ggaaaaatag ctgctcatat tttgccgttt tttgagctaa taccgaaggt cagctgattg
    15421 aacaccgttt accaagaggt tgtgagccta ccatttaact ccgaatcaca agaaataatt
    15481 tcaaaaacaa cttctcctgt ttttggttcg gttggcttat tatcgagaag tagctactgg
    15541 gagtctattt atcaaaaaaa ggaatgcgac ataggtcaca cccctttttt attttctttg
    15601 aaacatcttt cggtactcga cagcatacaa gacaaaagat tgtaaaagac cgtaagtgat
    15661 gacggcaaaa atgactctca cccacagcgt tggtaattct gggaaaagca gcaaaaggat
    15721 catgccaaca tagatgacag cagtcgctac tttgccatac cacttcgctc cacttaattt
    15781 gatattttta gatagcccga ttaatccagc tacaaacata aagccttctt ttatcaagaa
    15841 tagaccaaac aacagccaaa gccacggtcg ataccatgcc atagacaaaa tcagaaaaag
    15901 ttgtgtcaat ttatctgcaa acggatcaag caccttccct aagtctgtta cctgattata
    15961 tctccgagca atgaccccgt ctagataatc agacaaacct gagacaacaa tgaccgctcc
    16021 tgctatataa aatgactgtc tcagcacaaa ataaagataa atcggcagca aaaaaatacg
    16081 gaatatcgat aaaagattcg gaatcgtttg tatttcttta cgccagtctg tgcttaattt
    16141 cattttatcg ccctacctcc tcagcactct tttcttttta ttttagctta cacggggaat
    16201 aaaaccaatg atttcactgt ttttattttt ctttacagac actgatatta tgagctaatt
    16261 tgagataatc acttgctgtt ttcatcgttt actcttcttt cttcctgtcg tttgcgcatt
    16321 tcttttacta gtactttatg agcttttttt gatcctcgct ctaggtcacg ctgccgttta
    16381 cgtgttttat aatcgataaa cagactgtct aaatcgtaac ctgtcgggta cagatcttct
    16441 gctgaacgca ccagacgaac acgctgacga ggcacgtgga ttattttttt gtctaaatat
    16501 acatcaatcg tatcttcacc tatatcttca taaactaacg cttctttttg gtattcattg
    16561 acaaatatcc gatcaccttt tgcaaagagc tgacgttctt ttttattctc cttcctattt
    16621 gtaactgctg tcttaggaag aaattctttt ttctgtgtcc gataggcttt ttccgccaag
    16681 taacgctctg cttgatggat caatttcatc gacatatcca ttttatgagc aatccacaaa
    16741 gcttggcttt ctcctgtttc ttccaacccg caactgatat ttaggacgaa gtgcttcccg
    16801 atcgaaagcc atagcagccg gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc
    16861 tccataatgg gtcgtggcaa tgatcaatgc cccttgctca tacatagatt ccatgattgc
    16921 gatcgctagt gcagcccctt catttggctc tgttccactg ccaatctcat ctaaaagtac
    16981 caacgtattc ctttttatct ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa
    17041 tgtactcaac gcattttcta aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa
    17101 gacagcaagc tctgtccctt cttttgccgg aacttgcata ccgaacatcg ccatcaaagt
    17161 caataaaccg actgtcttca acacgactgt tttgcctccg gcatttgctc cggtgataat
    17221 caagcctcga tagtctttac cgatttcaac atccaatggt accgcatgct caggtaaaaa
    17281 tggatgtctg ccttgcttga taatgatatg ttccgattta ttgattctag gtgtgcttcc
    17341 attgatttct cggctaaatt ttgcccgagc aaagatgata tctaacaccg tgatcgtttc
    17401 gatcaataaa tcgatcactt cttcgttttc tgcgatagca ccagtcaatg ccgccaggac
    17461 ttgatattct tcactgattt cttcagcttt taacaactga tagtgttcat tcaatttaga
    17521 aatcacagtt ggttcaataa aaaccgtcgt tcttttattt gattcatcga taatcgttcc
    17581 atctatttta tttttatagc tggctttgat aggaatcgta tagtattcgc ctttttgaac
    17641 aatcattgct tcttggatca tttctttgtt tttcggatga cgcaagaatt tcagtaattt
    17701 tgactggatt tctttttcga ttagttgtaa ttgtttgcgt actttacgca aattacgtga
    17761 cgcgtcgtca ctgactttat tattttttat tttctggtca atcaactctt ctacatttat
    17821 caaatcagga agatgtttgc tataagcaaa caacaagggt gtctggtatt gattcttatc
    17881 aaaaaacttt gtgatcatcc gactgcttct aagaaaatcc gcatattcga tcaaatcggt
    17941 tggctgcaga atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc
    18001 cataaatgga acatgctgac tgctttctaa gatcaagcgt gcttctcttg tttcttcttg
    18061 acgatccaag acagtttgta ggtttgtcga gaccgtcata tcgctgattc gttttttgct
    18121 atagtttcca attgctcttg cttggatttc ctttttgatc cgatcaaatt gtaattgact
    18181 aatcatttcg ttgtccatat tctctccttt tcttgaagag atacctgtcc caaaataaat
    18241 aagggcctag agataaataa aaggatagaa cacagcttaa aaaagtcatg tcctatcctt
    18301 attagaatac aaagatattg ttttcctgac aggtaccgtt tcataagcac accaaataaa
    18361 ctttgtttat tggctattcg acttactgat tagataccag ctacactcac aaaacatacc
    18421 ccattcttct aggtcttatt tattttccac ttaactatag caagacacat tttggttgtc
    18481 aaactttagt atgttttata gatttacacc attaatgaat agacacctac cgcgataatt
    18541 gctccgatca ttggacctgc gattggtacc catgaatatg cccaatctga agttccttta
    18601 ttagctattg gcaataccgc atgtgctaat cgaggaccaa agtcccgagc agggttgatt
    18661 gcatatcctg ttggccctcc taatgaaaga ccgatggcta agatcaatcc acctacagcg
    18721 aatacgttcg ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc aagaactaat
    18781 acaaatgtac caattaattc tgtgatcaca tttgctggat aattccgtat agcaggacct
    18841 gttgcaaacg ttcctaaaat cgcgctttca tctttcgttg cattccaatg tggcaaataa
    18901 gaaagccaaa caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga
    18961 acgacaagat tccaactaat tgcccctgtc atcgccatag ctagagagac tgcaggattc
    19021 aaatgagctg ggcccatgaa tccagaaata tagacagcca ttgttacagc gagtccccaa
    19081 ccaaatgcga tgacaatcca gccagaagcc tgtgctttac ttttgtttaa attcacagcc
    19141 gcacacacac catctccaag caaaactagg atcagtgtcc caataaactc ccccatgatt
    19201 tgagtcatat cactgttcat ttatttctcc ccctttagtt cccgcagatc tgattctgca
    19261 atcaatgctt ctaaacgttt agtctgttgc gccttctcct cttctgtcca ttcaaaatat
    19321 ccagcaattg catcgatcac aggttgcttg atctcatcaa gctgatctcg ttcaaacaac
    19381 agatgatttg tacgacgaat gagataatcg cctggggcca aaaccatttc ttcttctaaa
    19441 ccataacgta accgagctga ctcagcgaga ctcaaaccag gataaggtgc catttctttt
    19501 gctaattcaa agatacgtcg ggcattagtc ccgtaaaaat cagcgatata agtagcatct
    19561 tcttcctcta aaccggctgc tactccttct tttgctaatt ctgtcactgt ttcttctaat
    19621 ttagttggat cgaagtttcc acctgaaatc tgatattttt tagagtcgat ttctttcgtc
    19681 tctattccgt attcttcttt taataattgg cgtatcaatc gcaaagctcc ttctgccatc
    19741 ttacggtaat cggtgatttt cccaccagat aaagtcagca aaccatctgg ttctctttct
    19801 aaagaactac ctctggaaat cgtagaaggt gctttttcat cacggctgtt ttccaaatgg
    19861 ttcaacacgt cttctacttc agcacgagaa actttgtttt ccttatattc acttactgta
    19921 tcgacaactt tattgaagct cttgtctgaa atcgatccat tatctccacc attgtaatca
    19981 gaaccagaat taccaatcaa cagtggacga agtcctgccc agcttgcttc gatatcattc
    20041 aatgtgatat ttgcttctgg atagcgatga ttgatcacat ccaatagata atctacatct
    20101 tcttgtgtga ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag
    20161 taagtcttgt tttctcttgg aatagcaaaa accatccgcc catcttgctt tcctgtatcg
    20221 aagtatgtgg gttgcggtac aggcagtttt ttcgcatcta caactaaatg gatcccttta
    20281 gttggacgca ttttaggaga gactggacgc gtaaaattca agtttcgtac tttatctacc
    20341 caaggaccac tcgtattgat cactaatttt gctttgattt cgatcacttc atctgtcagc
    20401 aggtcacgag ctttgacgcc aacgatttga tcgccatcat ataaaaagcc aaccgctttc
    20461 attttactta ctagataagc cccatcttcg gcagcctttt tgatattatc aatcactaaa
    20521 cgggcatcat tgttgcggaa atctagatag acacccgcgc cttttagccc ttcttttttc
    20581 aaaaacggtt ctctttccaa tacttcttct ggtgtcagcg tataattctc atatttggtt
    20641 cctgtcacat ttgccagttt gtcataaagg tccattgcta ctttgaccga gaacatattg
    20701 aaggttgttg ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg ttttgggatg
    20761 tgtggggcaa tactttgtac gactgcacgt tcaccaactg tgtcagctac tacttccaca
    20821 tcaaatgtct tcagataacg aataccgcca tgaacaagtt tggtcgagcg agaggaagtc
    20881 ccttctgcaa aatcctgcat ttcgatcaat cctgttttga ttcctgatgc tgctgactgg
    20941 actgccacac cggcaccagt gattccgcca ccgatgatca acagatccaa ttcttcttgc
    21001 tgcatttttt gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta
    21061 ttcaaatgct tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg
    21121 ctcttcctcc atttgaactt caaatagctt gccagcttca taaaattcgc ggatctcgtc
    21181 tgtatctttc caataaccga cagctaatcc tgctaaaaat gctgccccta gcgccgttgt
    21241 ttccaagttt tctgctcgtt ggatcggtac attcaagata tctgtttgga attgcatcag
    21301 atattcattg tttgctgcac cgccatctac tttcaataca ggaatcttga ttccagtgtc
    21361 atcttgcatc gtatcgatga tatctctcac ttgataagca atcgactgta aagtagcttt
    21421 aatgatatct tcttttgtcg taccacgtgt caagccaaac atagcccctc gtgctgcctg
    21481 atcccaataa ggagcaccta gcccaacgaa tgcaggaaca acataaacct catcttcgct
    21541 tgtagagcgt ttcgctgcgt cttctgaatc gctagctttt tgcaacattt gaagtccatc
    21601 tctcagccat tgaatcgaag aaccagccac aaaaatactt ccttctaaag catagtaaac
    21661 ctttccattt atgccgtaac caattgtcgt caataaatta tttttggata attgcggttt
    21721 ttcaccggta ttcatgacaa taaatgaacc tgtaccataa gtattcttga ccatgcctgg
    21781 ttcgaatgcc atttgtccga ataatgcggc ttgctggtct cctgccatcc ctgaaattgg
    21841 tgcttcgctt ccataaaaat gataaccttg cgtataacca taaacttctg agttagaagt
    21901 gacttttggt aacatcactc gaggaatatt tagtaaatct aaaatctcct gatcccaatc
    21961 tagctgatgg atattgaaca acatcgtccg actggcattc gagtaatcag taacatgtgc
    22021 ttgtccatcg gtcagtttcc atactaacca agaatcgatc gttccaaaca tcaattcgcc
    22081 tttttcagct cgttcttgcg cgccttctac atgatcaaga atccagcgta tcttcgttgc
    22141 tgagaaatag gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt gatagccttc
    22201 attttttaag ttatctgcaa ttccagctga ctgtctggat tgccaaacaa ctgcatgata
    22261 gattggacgt cctgtatctt tttcccatat tactgttgtt tcacgctggt tcgttatgcc
    22321 aatcccagca atctgatttg gtttgatccc tgattcgatg aacgctccgg caataactga
    22381 ctgtacagaa ttccagattt catttgcatc atgttccacc caactttctt gcgggaaata
    22441 ttgcgtgaac tctttttgag aactgccgat gtgtcttcct ttcttgtcaa agataattgc
    22501 tcgtgagcta gttgtacctt ggtcaatcgc catgatatac gttgattctg tcattgtgat
    22561 tccctccatt atatgcaaac gctttcttta actactctta tcatactact gttttaaaaa
    22621 aagaaactgt caaaatttta ttcttttttt ctttttttga cagtttttta ttatgaagct
    22681 attttttaat caattctttt cttttttcag cgtctctatt cttcttttca atcgaataat
    22741 gtcgtaaggt gacgcaaatt ctgacagata atattcacac tgttttttgt ttctctggca
    22801 aaatgtgatc accaaatcat attctttacc ttcttcataa cttccaatct caataccgcc
    22861 gattcccctc agttctctga tcagatattg ttgaaaagca atacgataaa tcggcagact
    22921 ttctaaatcg attccaatca ctactggttt tgcaatataa aaatcgatca tcaaaagaac
    22981 attggcatac tggatcatca ggtaactaag ctcactatct tcagctcttt tcctcggcaa
    23041 ttgctgcacc gctgtttcct gtaatttcgc cagtaactgt gttaaggaat gcccgagcat
    23101 tttctgctgc tgttccaaaa gccgatcacg ctcatagact tcgatctttc caacaaagaa
    23161 aaaccattca ttgttgatct gggaaatttg gtagcctact gccttttctt ctttgatttt
    23221 caaacgacga ggacgataat gccataacat cgtttcccga atgtaagtat ctaaaacagc
    23281 ggttggtaac tttttgctcc gtgtcaaatc atactgataa aatatttctt cgtctactat
    23341 cgaaaaagaa acaaaaaagc tgtaaaacat cattgtttcg tatacattca gttctgctgc
    23401 tgttcgacta agatgcatca cgatgattgg atcgattgct tgataaagcc ggtccgactg
    23461 atagagtaat ttcttttctt tcaaagttgc gtacgtactt ttttcattca atagtctttt
    23521 tttcgtaatc ccgagccagc aagcaatctg tgcttctgct gatgccgtaa acgttgtttc
    23581 taatacacgg tccaatccta gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag
    23641 cggtcgctga tcttccggga tatattggaa tagttcataa taaaaatatc gtatctgtag
    23701 ctcttctccg tataattgac cgtttttgat ttgtaatcca aattcctcta agagctggtt
    23761 caattctttg atctttcgaa aaacagaaga ctcactgata ttaaacgctg tcgctaatcg
    23821 aacgatggat acttgctgat gttctaataa atagacgagc atcttaaact tcaagctttc
    23881 ctgcaataaa aacaatagaa ttttttccaa acttacatct tcgtccaaca caagcagcgc
    23941 ctgatactca tttcgctgta cttctacttt tttttccatc attcggccaa tcaattcaat
    24001 atcttccaaa tattgatcaa acgaggcttt tgacagttgg acatgctctc tcaaaccatt
    24061 gatagaggct gttccccctt gcagaacaag gtttcgcaaa atcaggatct gtaactgctc
    24121 ttttctttct aataatgctt caatcttcat aggcagtatt cgccaccttt gaccacacca
    24181 tatctggcac acggtattct ggctggaaaa atagctcatg ttcttctaac tgttcaaacg
    24241 tccatccttc ttctactgca attgtcagtt ggtcaagaag gtggaacatc tcttttttgc
    24301 tttggagctg tgctccaaga atcttttttt cagaatcaaa tagaaatttg agtttaactt
    24361 tgctttgatt tattgctgta agcttgtagt ctacgtatcc tgtttcaatt ttttggggat
    24421 aaaaaatgct ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca aaccactgat
    24481 tgccgactgg acgataggtt ccctgatcct ttgtctgtga gccactaatt gtcttgctga
    24541 catttcgtgc tgtacggaca gcgttattta ctagagaaac atacattttt tcatttgttg
    24601 gacgaaaagt tacttggatc gcatcgccaa tcgcataaac gtctttttga gacgtttgca
    24661 agtagtcatc tacccagatc gttccatcat cattcaatgt taattgttct tcccacatct
    24721 gattgtcggg acgagaatga ttagccagca aaacaagatc atagttctcc tttctttttt
    24781 ccgtctctac aatagcggtt tcattttcgg tcaaagacac aacttgttct tctaaaaaaa
    24841 gtgaaagccc ttgatttttc atttccttta ctaacggttc tgtcatttca ggatctagat
    24901 aacgaaaaag aattgacttt cggctttcgt aaagatggat ttgttttttt cttgatgcca
    24961 acccttcagc tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat
    25021 tcatgatctt ttgctgaaga gccgttacgt tttgtatctt tttaactggg tgaataagcg
    25081 ttgattctgc agaaatattt cgaaactttt gatttgaacc agtcgctaga atcaaacgat
    25141 caaaatccaa tttttttcct cctgctaaaa cgactttttt ttctttcaat cctattactg
    25201 tcttttctgt gtaaacatgg attcgatagc ggtcagtcag ttcttctttc gtgatccaag
    25261 aaagttgttt ttcactggta acctttcctt ttaatatcaa attgaacccg ctgggaatcc
    25321 atcctaactt cgattgcttt tcaatcaaat atatttcact tgttggcatc aattggcgaa
    25381 gataaatagc cgcatgaatc cctgcaaacg acccgccgac aattacaatg ttcatctgca
    25441 ttcctctttc ttataaggtg ttctactgct aacttctcaa tcagaaaaca cagtattcat
    25501 aactcgcttt caaccttatg atacatagcg atgcagcaga atcaaccctc tccatcatac
    25561 cgtgttctag gtctgcttct caataatgta ctagtggacc taattgaaga gtatcatttc
    25621 tgaagagatg tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct tttacgtacc
    25681 tctgtccaaa taggaaacag aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat
    25741 agttagaata gacacatcat ttatttttaa ttctttaatg cttcaacaac tgcttcgcct
    25801 aattgatgag cagattgagg attttgccct gtaagaagcc gcccatcaat ttgaaccact
    25861 tctgaaaaag ctgctccaga ctgatagttt gctccttttt ctttcaaagc atcttctgct
    25921 aagaatggta cttcattcgt tgtaccattt gcttcctctt cttcattact gaacccagtc
    25981 actgtacgtc cagctaaaat agattttcca gactcatctg taactggcaa caatcctaca
    26041 acaccgtgac aaacagctga gaccagccct tcatttcgat agatttgtga agcgactttc
    26101 ccaagatcta ttgcttctgg aaaatcccac attgttccgt ggccaccagc ataataaacc
    26161 gctgcataat catttgcatc cacatcatca ggacgtaagg tatttcccaa ggcccgattt
    26221 ctgaaatctt catcttcata ataggcccaa tcgatttcat ccatattcaa aacactttgc
    26281 ggttctaaag gtacatatcc gcctttagga ctgacaaaat ctgcatcgat cccatttttt
    26341 actaatacat cataaaaatg agtcagttca cttaaccaca aacctgtagg acgctcgatc
    26401 ttttcgtact tttctacatt tgttaatacg atcaatactt tcattttccc atcctctttc
    26461 tataaaaatt gcgttattga taacgtgctt tcaagaaatt atataatggg cttggcaaat
    26521 aaagctcttt atatgctttt tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca
    26581 ataatttctc tgcccagtca ggatcgacga tcatttgctg tccaattgct gcaagttctg
    26641 catatttcaa tacctgctct acatcttcgc gattgcggat cccaccgaca ccaactaaag
    26701 ggatctttcc ttgaatcgtg ttatggacca cttcaagaat cgatttatcg ttgtatttat
    26761 cagaacgagc tattcgatca tacatattta atgaaacatg cagataatct agttttgact
    26821 cacgtaatcg ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt gtttcatatt
    26881 cttctggtga gaaacgatac cctacgatga atggtttttt agcatattgt tcaaccgctg
    26941 agaaaaccgt tgttaataat tcttcaataa aacgataacg attttccaag ctaccgcccc
    27001 attgatcctc tcgtctattc gaatgcggag agaaaaattg ttggatcaaa tacgtatttg
    27061 ctccatgcaa ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa
    27121 atgcttggat tgtttcaacg atttcttctt cactcattgc tcgtggtgtt tccgcatcag
    27181 gacgttttgc tgcaactgca cttgcagata caggctgttc tccagataaa gttgctcgac
    27241 ttgtcattcg tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct ccttggattg
    27301 ctttcgctag ctcacttaat cgaggaatca tttcatcatg agcgatgctg agttcacctg
    27361 gccagccttt tccgatatct tggacatttg cagcaccagt aataacagca ccaagacctt
    27421 tagaacgttg tttataataa gtgatctcat cttcggtgat cactccattg aaaaagctga
    27481 gttgtgtcgt cataggcgac agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag
    27541 taattgattg atcaaattgt gacattgtca ttcctcccat aattcgctct gattaaccta
    27601 tcgagaatca tctatcatta aaccactcga ttagttgtac atgcaactaa tttgcttaaa
    27661 aaattttatt ttttttgtaa cttatctgtt gcatcattca ttgtctgggc taattcttta
    27721 cttgcttcat ttcccaaaac tgctttgtat gactgatgaa gttcttccca gctaccatcg
    27781 acttccttag caaattggcg tccttgttct gtaagataaa ctaatgacaa gcgaccttcg
    27841 ctaactgttt tgacgtatcc tttatattct aatttttcaa caaaacgagc aatcgttgat
    27901 ggggcggaat agagcatttg tgcaatatct ttttgtggta ttccatcttt ttcttttaat
    27961 atcaataaaa taaaaccata agttggtgcc agacctgtcc ttctaaacgc ttcatcagct
    28021 aatttattca acatgcgatc tagctttttc acgctaaaga aaagacattc atctaacgct
    28081 ttttcaattg gcttttccat ttcatcccct ccattggcgt acatatcata gtatgatttt
    28141 tattttttgt caaaaaacac ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag
    28201 aagtagccat cttcaaagaa tttcggcttg ttttcgacaa aactccttct ggaacaccgt
    28261 gaatcagagg ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat
    28321 ggctttccac atttttgcat aaatttggct taataccgaa aaactggctt ttgaacaccg
    28381 tttatatcag aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa
    28441 aatagttcct catattttgt cgttttttga gctaatgccg atggtcagct gattgtacac
    28501 cgtttattga gaggtcgtga gcctgccgtt gaactccgag tcacaaggag caatttcaaa
    28561 aaacagcttc tcatgtttta tgactatggt gacttgtgat cgaggagttg gcaggagaat
    28621 actgtttatt cgtaaagaag gggctgtgac aagacttttg tcacagtccc ttatttcctt
    28681 ttattatagg atttcgccat ttgattggat gacttgtctg taccaatcaa atgatttttt
    28741 ctttaaccgt ttctttgtgc cattcccttc gtcatcttga tcaacataga tatatccata
    28801 acgttttgac atttgtgaag tagaagcgct gacgatatca atgcagcccc acgacgtata
    28861 accaaataaa tctactccat ctttgatagc ttccttcatc tgctcaatat gctgcttcaa
    28921 atagtcaatg cggtaatcgt cttgaacggt tccatcttct tctactttgt ctatagctcc
    28981 gagtccgttt tcagctatga acagtggttt ttgataccta tcatataatt gattcaaagc
    29041 aattctcaaa cccttaggat caatctgcca gccccattca cttgatggaa gatacggatt
    29101 tttaattcct ccagttatta agtttccttg gaccccttcc caattttctg gatgagcact
    29161 gactgcacta gacatgtagt agctaaaaga aacaaaatca acagggtagg ctgctaagat
    29221 ttcttcatca cctacttcta tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt
    29281 atacgctgga taatatcctc ttgcttgaac atcactaaag aacaaagaag ttctttgctg
    29341 ctcaattgtt ttttgaatat catccggatc acaagaatac ggaacaagaa gctgatagct
    29401 gatcatacaa ccgacttgtg aacctttgat aatttcatga caagcttttg tagctaaagc
    29461 acttgctata aactgatggt gtgcaccttg gtatttcgct tgttccaaat ggggatggtt
    29521 ttcttcaatt actcctacgc taacaaaagg gtgatgtttt acacagttga tctcattgaa
    29581 agtcatccaa tattttactt tatttttata gcgtttgaat actgtttcag cgaaacgaac
    29641 gtaaaactga ataagctttc gattaggcca tccaccatat tctgtaacca aatataacgg
    29701 catttcataa tgggaaagag aaacgactgg ctcaatccca tattttagac attcgtcaaa
    29761 cacttgatca taaaaaaata atcccgcttc attgggtgtt tcttcatcac catgaggaaa
    29821 aattcttgtc catgcgatag acatccgata gcatttaaaa cccatttcag caaataaacg
    29881 gatgtcttct ttaaagtgat gatagaaatc atttccgtgt cttttaggat aataatactt
    29941 gctgttggga tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc attgatctgt
    30001 ccatttttct ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag ataatccttt
    30061 ttcatctaca tcccatgcac cttcacattg attggccgct gtcgctccgc cccataaaaa
    30121 tccttctgga aattttttca tatcgatccc cctaaacttg ctagttttct tgtttgatta
    30181 tttttcttat gactagtaat gatatttttg aatctactac tatttgtaac aataaccatt
    30241 gtggtcggat catatccctc tttgattaat tcttgcaaat ctacttccat caataaatcg
    30301 cctttacgta ctttttttcc atcagatact gcactttgaa agtatttgcc atttaaattc
    30361 actgtatcaa taccgatatg gatcagtatc tctgctcctt cttcactgac taccccaatt
    30421 gcatgtttcg ttggaaaaac tgttgaaact tccccgttta cagaactgta gatttttccg
    30481 tccgttggaa tgatcgcaaa cccttctcct aaagatccac tagcaaatgt tggatcgttt
    30541 acttcagata aatcaattat ttcacctgct gcaggagtac ttaacactat atcctttgag
    30601 gattcttctt cgatttcatc cttgtagccg aaaagaaaag ttagcataca ggaactgatc
    30661 atcgctaaga aataactagc aactagccaa acaaatcctt caccaatata aatcgggaaa
    30721 gacaaaatac ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc agctactcca
    30781 ccaaatatag ctccacatat acaagcaatg taaaatggtc gtttatattt aagagtcaca
    30841 ccataaacag caggttcagt gattccaaat aatgctgtga taaatgcaga taaagataaa
    30901 ctttttactt tcttattttt cgttttcaaa aataccccaa gtactgctcc tgcttgcgca
    30961 gctacggcag tcatacatgc tggacctagc gtatcacgac cgtagaaaga aagattgttg
    31021 gtcattactg gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg
    31081 ccgataacag caccagataa aacagggcta aagttataaa cagctaaata tccagctgca
    31141 atcagctgac ttgcataatc tgcaattgga cctacaacta ataaaataat tgggaccatc
    31201 acgatgatac aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga
    31261 atcttaggct caatataaga aagaacccat acagctaaaa ttgctggaat aaccgtactt
    31321 gtataccttg ctagaatcac gggcatccct agaaatcgca aggttatgct atcactatat
    31381 gcactgacaa tcgtaggata aacgagagct gctgcaataa ccatagcgat gaaacgatct
    31441 gtattgaact tttttgcagc agtatatgcc aaaaatatag gcaagaaata gaaaaatgca
    31501 tcagctgctg cattcaaaat gatataagtt cctgattcag tagttaataa attcaaactc
    31561 gtacataagg cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg
    31621 gcaaaaacag cggcgatcat gtctataaaa gagtttaaaa tgcctttttt ctcagttcct
    31681 ctctttccac tagatgcatc atcttctagt ttcatttgtt tggataatac gtcatacact
    31741 tcatttactg catttcctac tacgacttga aacattcctc cagcttcgac tacagtgatc
    31801 acaccttcta gttgctctac cttctcctta tctgccttct tattatcttt tagggtaaag
    31861 cgtaaccttg ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg
    31921 acaatatctt tcgctaattg tgaatagtcc atttttactt cccccttgtt aattaatgcc
    31981 gtagcaatcc ttcttacaag ataagtatag ttcttagaaa acggtttctc cattcaatta
    32041 gtaccccttc taatagcgaa cgaaactgta caatagtgtg tatggtttac ttagccgagc
    32101 aaatcactca ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc aaaggagaca
    32161 aaaaaaacac gcttgatctt cctttgctga tgtataagtg gtatctcaag ctgatactct
    32221 gtgatttttg tttctacttg gcttttgata agacagcaca acaaagtctc ggcatttaac
    32281 ggaagcataa ctaggtcaga actaggagag attagttgct gaatggaatc tatcttttta
    32341 tggattttta tttgattttc ttctttgtag taatgatcaa gcagttttgt tttctttttt
    32401 tcttcaacta tggtcagctt ttcacgcaaa atctgataaa tctcccaaga ttcaaactca
    32461 tctagaacac taggtagata aatcaaatca tcccactcaa aaataagctc ttgctcagta
    32521 gtcaaaattg catcatattc cgttattttg tttgattgat ttttgaaaag atacactggt
    32581 tctatccggc attctttgat ccacatgttc atactttgtt gaatctgttt ttttagtgta
    32641 ttcaaaatac taattggctg gttactaaca atcagtatct ccccttgttt tcgaaaagga
    32701 ttcaacgctt cggcaagata caaaacaaaa taagcgcttt cagctaaatt gggacgtaat
    32761 tgaaaatgta ttgggaagaa acgccgaatt aaataaattt ctaaaggata acgggagatg
    32821 atcttttgta caaaatgatt catggtatta tgtccggcat ccatcctcgt tttcatttgt
    32881 ttgatatggg ttagcaaaaa ttctgtttca tggaacaata acgaaacagg aagatctaaa
    32941 aaacgaataa tcgtttgctc aaatgctctt agattcttct ctatttcttt gtttttttca
    33001 tcattaatca tcaaataatt taactctaat agtcgatttt caattgcttg tttttcttga
    33061 ttggaaaaag aatagggtag ttctgatgat aacttttttg tgagggtctc taccatcgga
    33121 ataaataatg tatacctgtt tgttttttct aaaagttctt ctcttcgaag aatggtcaaa
    33181 acgagatacc gagaaaatcg tatgaagtcc tctcctgaaa caatataatt atatgatata
    33241 agaacctctt ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat tgtactgatt
    33301 tgttgaggaa accttgcgtg ttggatcacc gtttctgttc caacaagaga taagaaaatt
    33361 ctttttgaat tttcttcagc aatgattttt agtccttttg tcgacgaaac ttccagttct
    33421 aatttaggat tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga tacggctgtt
    33481 tttgaaagaa agaaaatgtc tgctatcttt tgcatactga catacccttt ttgaaacaat
    33541 aagtacgtca caatagttga agctcggaaa tccatgctgg aatagatttt atgtttcgtc
    33601 acctcttgta tgatttgctg tttagtttca gattcaattg attctaaaaa ataacctgaa
    33661 cgtttatttg actggataat gattttcttc tcgtagatag caagcatatt tttgatttca
    33721 gtttggatcg ttttcttgtt ggtacctaaa gcttcagcta attcttcgct tgttatccaa
    33781 tgattcttat ttcttaatac tacaagaatt tggagttgtc gtttatttaa gtccatttgc
    33841 ttacacctcc tctttgttat aaacatacag gaaaaacctc tcaaatgaca atagtcactt
    33901 gcagaggttt aaaaatcatt tgaagaaaga tctatattca aggtttttac cattagactg
    33961 gataatttgt ttataatatt caaaactatc ttttttcaat ctcctaaagc ttccgctccc
    34021 atcattttgt ctatccacat aaataaagcc atatcgcttc ctcatttccc ctgttcctgc
    34081 cgatacgata tcgattggtc cccaccataa gtaaccaatg atttggcaac catcttttat
    34141 tgcctcagca atttcctgaa gatgtatttt tagatactca caacgttttg gatcgtggat
    34201 tcgattcaat tcatctggtg cttcatctag tccaatgcca ttttcaacta tgaataatgg
    34261 taattcataa cggtctgtca attcattcat gacataacga aaccctagag gatcaattgg
    34321 ccaactccaa ggttcgggag atttttcctt caaaaaggga ttatcgaaac catttgctcc
    34381 gccagtatct acgcgcattt ctgcttcttt tttatagaca gcgctacgat aataactaaa
    34441 tgcaatataa tctgccgtgt tagcttgcaa gcactgttcg tctccatcta acattactgg
    34501 tgcacagtct tcttccttcc atgtcctgta aatataagct ggataatgcc ctcggcacat
    34561 gacatcggag aaaaaccatg aattgcgttt aaattgcagt gaaccaaata cattctctgg
    34621 atcacagtca tatgcataag tcgctaatgc agaaaagcta cacataatac ccatcttagc
    34681 atttggatcg attttcttta ataattgagt agcacgggcg ttcgctagaa acatatgatg
    34741 acaggcttga taaatcactt tttttgttag tgtagcaatg ggttcaagtt tatctctagc
    34801 agggaatgta accaatccag cagctgcata aggcatacga aaagcattat tgatctcatt
    34861 aaaggtcatc caatatttca ctttcccttg gtagcgttca aagacggtct gcgcataaaa
    34921 ttcaaaaaag tcgattaatt ttcgattttg ccacccccca tactctccta ccaatgctaa
    34981 gggggtttca taatgactaa gagtaattac tggctcgata tcatatcgat ttaatgtatt
    35041 gattagtctg tcataaaata cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg
    35101 aaatattcgt gcccatgaga tgcttgttcg aaaagctttt aaacctaact ccttcagtaa
    35161 acgaatatcc tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa gataatggag
    35221 tgattcgtct aaagcaatct cccaaatctt tttttcttca ttccactgaa tactaggatg
    35281 ctggttcatg atcccgataa gcgtatctgt cacattaggt aatttgccgt cttccaacca
    35341 tgcaccttca cattgattag cagctactgc tccgccccat aaaaaatttt ctggaaatat
    35401 ttcctgatcc ataactatct ctcctcgcta tcaacaattt cttacttgaa gtatagctcg
    35461 attcatcaaa acaagatatt ctaactattt cggcagaaga gaagaaaaaa attgaatgct
    35521 actcttcatt tttaatattt atatataaca aatacataag acgatccccg aaagaaaaca
    35581 ctcgttaacc atctaagcaa acgatttatt ttacttccaa cacaaatgct tcataaggtc
    35641 gtaaatcaac tttgccgtct ggtaactgtt tcagaggata atttgacaca ataaccctta
    35701 aagcttcacg tggaatagtg aacgtctgct tttcactgga catattacaa ataacgagcc
    35761 attgctggtc ttcatatatg cgagaatagg caaagacatg ctcaggcgtc ttttccacta
    35821 attcatagtc tccccagacg atgatgtcat gttttctacg caaatcgatc aatttcttgt
    35881 acatataaaa aacagaatct tgatctgcta acgcttcttc tgcattgatt tctttataat
    35941 ttggattgac tcgaagccat ggaattccag tagtaaaccc agcatgttca cgggcgttcc
    36001 attgcatagg cgtcctagca ttgtctcttc ctttcgcctt gattgattcc aagatttcct
    36061 cttttgtaaa tccagaagaa atcctttcgt tatacatatt gattgtctca atatcttttg
    36121 cttccgcgat atcatcgatt gggcaattcg tcatgcctaa ctcttctcct tgatagatat
    36181 aaggggtgcc tttcatcata tggagcaaga tagcaaacat cttggcactt tcttttcgat
    36241 acacatcgtc atttccccaa cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata
    36301 aactattcca tccttcatca cctaaagatg tttgccattt ggataatact tttttcagtt
    36361 cagagatact caagggtttc aaatcccatt tgtctttgcc ttcttgctga tctaatccta
    36421 tgtgttcgaa ttggaagacc atggataatt catttctttt aggattggaa taaagtttag
    36481 cgatttcggt agttgctccc catgtttcgc caacagtcat cagatttttg tcaccaaaag
    36541 ttgcttgatt catttcctga agatactcat gcagtttagg tccattactt gtgatttctt
    36601 tatccggttc tttgccaatc atatcgatca cgtccatacg aaagccccca atacctttat
    36661 cgatccagaa attcatcatt tgataaatct cttggcgaac ctgtttattt ttccaattca
    36721 aatcaggttg acgcttgcta tacaagtgca gaaaatattg ccccgttttt tcatccaact
    36781 gccaagcaga accactgaat attgagcgca atctattagg ctcttttcca ttgaccggat
    36841 cacgccaaat ataatagtcc cgataaggat tttctttatt tttacgggcc tcaatgaacc
    36901 acggatgttc atcagaagta tgattcacca ccagatccat gatgatacgg atatttcttt
    36961 tctttgcttc ttcaatcagt tcttccattt cttccatcgt accgaattct gccatgattg
    37021 cttgataatt actgatatca tacccattgt catcattcgg agactcgtat actggagaaa
    37081 gccaaattgc atcaatacct aaatacgcca gataatccaa cctggaaata atcccccgca
    37141 aatctccaat accatcttga tttgaatctt gaaaacttct tggatagatt tgataaacaa
    37201 ctgaatggtg ccaccatttt tgttccatga ttttcctcct cgtaaaatat catttgtcta
    37261 atagttttta ttcaaaatgt ttctagatta taaaatttat tgaataacac attcctaacg
    37321 gaatttgttt cgatattgtg tgatttctca gagtacaatg gatgtatgga aaggatccaa
    37381 cgggaatttg ctatttcctc ctgtagcgtt tgtctaccct gtttagtctg ttacccgaac
    37441 cactgttatc actaccaact cattagctgt ttcgataatg gactagactt tcaaatagta
    37501 gtttggaagg cagcttctag ctcttttatc accgtattct attaggcgag gttgtggcta
    37561 tacagtgagt ttctgggaaa tcccacagta tcgaagcatt ttccaaaatt tatatcgaaa
    37621 ggaggtcctt cttccatgaa attatttgtc ggtattgacg ttagctctga aaaactagat
    37681 gtttgttttt taacagacga caatcaattg tctatcttat ctgaaatctc tgttgccaat
    37741 gacatcgaag gtgcttcatt catacga
//
LOCUS       DACCXO010000017        36208 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.017, whole genome shotgun sequence.
ACCESSION   DACCXO010000017 DACCXO010000000
VERSION     DACCXO010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.017"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            258..533
                     /locus_tag="IVY70_000797"
     CDS             258..533
                     /locus_tag="IVY70_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957948.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            546..2624
                     /locus_tag="IVY70_000798"
     CDS             546..2624
                     /locus_tag="IVY70_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP7957949.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2784..4670
                     /locus_tag="IVY70_000799"
     CDS             2784..4670
                     /locus_tag="IVY70_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7957950.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4682..5629
                     /locus_tag="IVY70_000800"
     CDS             4682..5629
                     /locus_tag="IVY70_000800"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP7957951.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5649..6176
                     /locus_tag="IVY70_000801"
     CDS             5649..6176
                     /locus_tag="IVY70_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7957952.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6239..6892)
                     /locus_tag="IVY70_000802"
     CDS             complement(6239..6892)
                     /locus_tag="IVY70_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP7957953.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7024..7866
                     /locus_tag="IVY70_000803"
     CDS             7024..7866
                     /locus_tag="IVY70_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7957954.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8024..8920
                     /locus_tag="IVY70_000804"
     CDS             8024..8920
                     /locus_tag="IVY70_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7957955.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8923..9636
                     /locus_tag="IVY70_000805"
     CDS             8923..9636
                     /locus_tag="IVY70_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP7957956.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9656..10174
                     /gene="msrA"
                     /locus_tag="IVY70_000806"
     CDS             9656..10174
                     /gene="msrA"
                     /locus_tag="IVY70_000806"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7957957.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQDYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10171..10398
                     /locus_tag="IVY70_000807"
     CDS             10171..10398
                     /locus_tag="IVY70_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP7957958.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10550..11101
                     /gene="lepB"
                     /locus_tag="IVY70_000808"
     CDS             10550..11101
                     /gene="lepB"
                     /locus_tag="IVY70_000808"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7957959.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11184..11726)
                     /locus_tag="IVY70_000809"
     CDS             complement(11184..11726)
                     /locus_tag="IVY70_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP7957960.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11963..12361)
                     /locus_tag="IVY70_000810"
     CDS             complement(11963..12361)
                     /locus_tag="IVY70_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7957961.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12621..13481
                     /gene="ylqF"
                     /locus_tag="IVY70_000811"
     CDS             12621..13481
                     /gene="ylqF"
                     /locus_tag="IVY70_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP7957962.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13474..14241
                     /locus_tag="IVY70_000812"
     CDS             13474..14241
                     /locus_tag="IVY70_000812"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP7957963.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14297..15154
                     /gene="dprA"
                     /locus_tag="IVY70_000813"
     CDS             14297..15154
                     /gene="dprA"
                     /locus_tag="IVY70_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP7957964.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15276..17354
                     /gene="topA"
                     /locus_tag="IVY70_000814"
     CDS             15276..17354
                     /gene="topA"
                     /locus_tag="IVY70_000814"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP7957965.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17323..18699
                     /gene="trmFO"
                     /locus_tag="IVY70_000815"
     CDS             17323..18699
                     /gene="trmFO"
                     /locus_tag="IVY70_000815"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP7957966.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18897..19802
                     /gene="xerC"
                     /locus_tag="IVY70_000816"
     CDS             18897..19802
                     /gene="xerC"
                     /locus_tag="IVY70_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP7957967.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19839..20387
                     /gene="hslV"
                     /locus_tag="IVY70_000817"
     CDS             19839..20387
                     /gene="hslV"
                     /locus_tag="IVY70_000817"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP7957968.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20401..21801
                     /gene="hslU"
                     /locus_tag="IVY70_000818"
     CDS             20401..21801
                     /gene="hslU"
                     /locus_tag="IVY70_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP7957969.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21819..22610
                     /gene="codY"
                     /locus_tag="IVY70_000819"
     CDS             21819..22610
                     /gene="codY"
                     /locus_tag="IVY70_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP7957970.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22692..23567
                     /locus_tag="IVY70_000820"
     CDS             22692..23567
                     /locus_tag="IVY70_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP7957971.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(23699..24337)
                     /gene="plsY"
                     /locus_tag="IVY70_000821"
     CDS             complement(23699..24337)
                     /gene="plsY"
                     /locus_tag="IVY70_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP7957972.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            24464..24898
                     /locus_tag="IVY70_000822"
     CDS             24464..24898
                     /locus_tag="IVY70_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP7957973.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25064..27109
                     /gene="parE"
                     /locus_tag="IVY70_000823"
     CDS             25064..27109
                     /gene="parE"
                     /locus_tag="IVY70_000823"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP7957974.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            27121..29571
                     /gene="parC"
                     /locus_tag="IVY70_000824"
     CDS             27121..29571
                     /gene="parC"
                     /locus_tag="IVY70_000824"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP7957975.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            29848..32079
                     /gene="pflB"
                     /locus_tag="IVY70_000825"
     CDS             29848..32079
                     /gene="pflB"
                     /locus_tag="IVY70_000825"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP7957976.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            32323..33084
                     /gene="pflA"
                     /locus_tag="IVY70_000826"
     CDS             32323..33084
                     /gene="pflA"
                     /locus_tag="IVY70_000826"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP7957977.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            33169..34095
                     /locus_tag="IVY70_000827"
     CDS             33169..34095
                     /locus_tag="IVY70_000827"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP7957978.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            34277..35170
                     /locus_tag="IVY70_000828"
     CDS             34277..35170
                     /locus_tag="IVY70_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP7957979.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            35304..35429
                     /locus_tag="IVY70_000829"
     CDS             35304..35429
                     /locus_tag="IVY70_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7957980.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(35563..36195)
                     /locus_tag="IVY70_000830"
     CDS             complement(35563..36195)
                     /locus_tag="IVY70_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957981.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT        12698 a         6209 c         7616 g         9685 t
ORIGIN      
        1 atttttgtcg cagccccatt tttttgttgt gataaaagaa caagaaaggc aggaatggga
       61 aagtaaacat ccttttgtca gaaactttta tctttcttaa gttttacttg atatctgata
      121 ctatttggaa tagaatgagg cgttgaaaaa tttatggaaa tttctaacta gcaataaacg
      181 atgaaacaat gaaatatttt tcatccaaaa agaactttgc tataatgatg gaagtttgtg
      241 tataggagat ctggaaaatg agagagagtt ttgaacaaca aaaaaaactg ttgcatgatc
      301 gctatggcgc cctttcgatg gatgaccgcc gtcaaatact ttgtaagttg cgtaaaagaa
      361 atatattgat gtatcgtcag ttggaacgtc tgaaacatga tctgcttcgt ttggagtcaa
      421 aacgtgtaca gtgtgaatta gaggggaacc aaacacaggt tgaagtagtc gaaaccaaaa
      481 ttttaaagaa aaaagaacaa tttttaaaaa tgcttacaca aaataaaaaa taaaggggat
      541 ggacgatgga atttgctgag ttagttattg tatttgcagt aacgatcacc ttatcgaaca
      601 tagctagccg cattttgcca atgattccag ctccactgat ccaaattttc cttggagtgg
      661 tattaggtct gacagagtgg ggacaatcaa tcgattttga acctgaattg ttcttagtta
      721 tgattattgc accattgtta tttagagaag gagaaaaagc agatatttct tcgattctca
      781 aaaatttcgg gacaattttg ttcttggcat ttggaggagt cattttgacc cttgtcgggg
      841 taggtgcgac gttatcattc ctgttgccaa gtgtcccact tgctgcctgc tttgcttttg
      901 gagcagcgtt aggtccgaca gatgcggtag cagtcagttc tctttctgga cgagtgaata
      961 tccctaaaaa ggcgatgcat attttggaag gagaaggtct tttgaatgat gcatctggtg
     1021 ttactgcctt ccagtttgct cttggtgcgc tgatcaccgg atctttttca gcagtaaacg
     1081 cggggatgag tctagtagtt tctagtattg ggggagcttt gatcggcttt ttgcttgtct
     1141 ggttcaaaca aaaaataatt catttgatcg aaaaagcatc agcacaagat gtcacagggt
     1201 atctgctgat cgaacttttg cttccttttc tagcctatgt attggcagag gcatttgatg
     1261 tatctggaat cattgctgca gtggcagcag gtattctcca agcttctggt tttaggaaaa
     1321 tctctgtgtt tgatgcagaa ttgtcgagct tgtcccacag tacatggaca actattgctt
     1381 ttacactgaa tgcattagta tttatatttt taggaatcga attgacgcaa gtgttctcgc
     1441 cagtatgggg agacggttta tatccaaacg gtcttttatt agcgattatc gtattaatct
     1501 ctgtcatgct ttttgtgatc agattcattt ctatcagtct tttttatgta ttcaaagatg
     1561 gcagtaaaaa gttcaaaaaa cagttgaacg agatcttgat cttaacattt ggcggtgtaa
     1621 agggaacagt cagtttggct acgattttta ttttgccatt ttcaatcaat aatatgatgt
     1681 tttaccagcg gtctttgctt ttgtttctga cggcgggtgt catcttagtg actttggtca
     1741 tagggatcat tgtcttgcct atgctgacgg aaacagagga agcgaaaagc acggatttga
     1801 atgctttgat gattttagaa gaggtcgttg aagttttgcg aaaagaaata aaggaaattg
     1861 atcgtaatac aaaagaattt ttagccactg aagcagtgat agaaaattat caagaacgga
     1921 tacgtgatct gtacttggaa gatttaaccg atgatgaaaa gcaagaagta caagaaatac
     1981 aagcactgat tttgtcgatc gaacgggatg gattggatga aagttatcgt tcgggcaagc
     2041 tttcttctaa tggttatcgt ttttactccc gttttctttc tcgattcgaa cattctatca
     2101 caagccagat tttgtctttt atcggctttt ggttcattgt tgtccgtagg ttaatgcgaa
     2161 tcgttctcca tccgaaaatg ttttggcaaa gaagatatgc tgatcgtcag acatttatta
     2221 gtgatgaaga tatccaagaa atcagagaga catatcaaaa aaatactcag ttgattattg
     2281 aaagtttaga taacttgact gatatttatg atgaaacgat ggttcaattt tttatccagc
     2341 aacgcaaact tgaaggaata aaaatgaact cggggaattt gatttcttcg tggatgatcc
     2401 agcaagatag tctgtttacg aaaaaaatgc taagaggtta ttatcttgaa agaaaaatca
     2461 ttgatgaata cgaagttgct gaaaaaatca cgacgttttc tgcgaacagc tatcgcagga
     2521 atatcaattt gttagaatct tatacgatga acaaaccatc tgataacttt tctttccgat
     2581 ttgcgtttcg agcaaagtca aaaaaaacga gtccaataaa ataaaaaggt aaaaattttc
     2641 aagttttata aaaaacaaaa catatcagaa caggatcgaa tcggtcctgt tctttttttg
     2701 agaacacaaa aagaacaaat accaaaaaaa ttttcctgaa gttcactatt tttatgatac
     2761 aatatccaag ataaggaggg aacatgttga aagaattaaa agtaactgac ctcaaaaaaa
     2821 cttatggtga aaaagatttg tttgaccaga tttctttttt ggtccacgaa aaagaccgta
     2881 tcgggttgat tgggacgaac gggacgggta aaacaagtct cttaaatatt attgccggta
     2941 tcgatagcag cgacggtgat cggcaaacag tgttttatcc aactgattac cgtattggtt
     3001 atctttctca agcagctgaa ttttccgatg aattaactgt tctccaagca gtctttcaag
     3061 gaaacagccc cttgatccaa actgttcgag catatgagga agcattgatc gaactagggg
     3121 aaaacggaga agaccctgat gtgcaaaaac gttatgctaa agcagaagaa caaatgaata
     3181 aagaagatgc ttggactaca gataccaacg caaagattat tttgcaaaaa ttaggaatca
     3241 gtgaattaga taaaaaaatc agtactttat caggtggtca gaaaaaacga gttagtctgg
     3301 cgcaagtgtt gattgatgaa cccgatttac tattactaga cgaaccaaca aaccatttag
     3361 attatgaagc gatcgagtgg ttggaaaact atttgaaaca ataccgtggc gcactattga
     3421 tggttaccca cgatcgttat tttctagata gagtagcaaa ccgaatcttc gaactttcat
     3481 ttggaaaatt atacgagtat aaaggaaatt atgaagctta cgtcttagaa aaagcagaac
     3541 gtgatcgtgt agaagtagaa caagaagaaa agcgcaagcg gctttataaa caggaattag
     3601 cttggatgag ggctggcgta caggcacgtg gcaccaagca gcaagcacgg atcaaccgtt
     3661 tcgaagatct aaaggagaac ttgtaccaag taaaccaaga agatgattta gagctgaacc
     3721 tagcgacaca acgtttaggg aaaaaagtac tggaaatcaa agatggcagt taccgtataa
     3781 atgaccaaac actgctagaa catttagatt tattgattca atctcgggaa agactaggta
     3841 ttactggtaa aaatggtgcg ggaaaatcga cattgcttaa tattctagct ggcagaattc
     3901 ccctggatag cggtacaatg tctatcggtg aaacggttca tcttgcgtat tatacacaag
     3961 aaaatgaaga aatggcacca gataaacgga tgattgctta cttacaagaa gctgctgaag
     4021 aagcaaaaac agcagatggc agtcaaatcg gagtagcaga attacttgaa cgatttttat
     4081 ttccaagatt tatgcatggg acactgatag gcaaactttc aggcggagaa aaacgccggt
     4141 tatttttgtt gaaactgctg attcagcaac ctaatgtttt attattggat gaaccgacca
     4201 atgatttgga catagcaaca ttgacgattt tggaagatta ttttagaagt tttccaggtg
     4261 ctgtgatcac tgtttcccat gaccgttatt tcttggacaa ggtagcagat aagttattag
     4321 tattccaagg aaacggaaaa caagaacttt attacggaaa catgtccagt tatttactga
     4381 agcaaaaaga gacccagcag cctgctgaaa aagcgaaacc aaaaatacag agcaaggaac
     4441 ctgctgaaaa gaagaaatta tcttacatgg aacaaaaaga atgggaaacg attgaagatg
     4501 agattgcaga acttgaagaa aaaatctcat tgctccaaga agagatgaat catcaaggag
     4561 ataatttcac tcgattacaa gaattgcaaa acgacgtatc tgaaacagaa gcacaacttg
     4621 aagaaaaaat ggcacggtgg gaatacttga gtgaatgggt agaagattag gaggaaaaag
     4681 gatggaacaa gcgtatcttg acttaggaag aaaattgttg gaagaagggc atcgtaaaga
     4741 agacagaaca ggcacaggca cattaagctt attcggatac caaatgcggt ttgatctgca
     4801 aaaagggttt cctttgttaa caacgaaacg agtacctttt ggattgatca aaagcgaatt
     4861 gctttggttt ttaaaaggag atacgaatat ccgctatcta ttacaaaata aaaaccatat
     4921 ttgggatgaa tgggcatttg aacgctacat caaaagtcca gactataatg gaccagatat
     4981 gactgatttt ggccatagat gtttgacaga tcctgaattc aatgaagaat ataaaaagca
     5041 gagcaagctt ttttgcgaga agatcctaac cgatgattcc tttgctgaaa catacggcga
     5101 tcttggacac atctacggct atcaatggcg tcactgggaa acaaaagacg gtggctttat
     5161 cgatcaaatc aaacaagtga tcgaagcaat caaaaagact cctgattcta gaagattgat
     5221 cgtttctgca tggaatccgg aagatgttcc tagcatggct ttgcctcctt gtcatacgat
     5281 gtttcaattt tatgttcaag agggacggct aagctgccaa ttgtatcaac gcagtgggga
     5341 tgtgtttcta ggcgttccgt tcaacattgc aagttatgcg ttgctgactc acctaattgc
     5401 acatgaaaca ggtttagcgg ttggcgaatt tgttcacaca ttaggagatg ctcatcttta
     5461 tatcaatcat atcgaacaaa tgaaagaaca attgagccga gaaatacgcg catttccgac
     5521 tttagttttg aatcaggaaa agcagtcagt atttgatttt gagatggaag atatccaatt
     5581 agaaggatat gacccacatc cggcgatcaa agctcctatc gctgtttaga atcagaaagg
     5641 atgagaaaat gttagctgca atttgggcac aggatgaaaa cggattgatt ggaaaagaag
     5701 accgtcttcc ttggcgtttg ccaaatgatc tgaaattctt caaacaaatg acagaagcaa
     5761 atacactagt gatgggacga aaaacattcg aagggatggg tagccgtccg ttgccgaatc
     5821 gtcagacgat tgttttgacg agagattctt cctataaagc agaaggcgtg catgtcatgc
     5881 atagtgtgga agacgtgctt gctctggaaa aagaaacaga cggtatcttt ttcatcgctg
     5941 gagggtcagc tgtttatcaa gaatttctgc cattttgtac gatattatac cgtacagtca
     6001 tccatcatgt attcgatgga gatgcttatt ttccacctgt tgactggtct gattggtcac
     6061 tgatcaatac tagtcaagga gaaatggatg aaaaaaatcg ttatccgcat caatttgaaa
     6121 cgtatcagcg taatgagaat cctgtaaaaa gcgggacagg cgaattgacg atataaaaaa
     6181 gctatggata gactagctgt tatttagagc tgtcccatcc atagctttat tttttttatt
     6241 aagtaaagaa taaaacagag aagtacataa aacctgcacc taacataaca aacaaatgcc
     6301 aaacaacatg catatagcgg atagaattca agctataaaa gaatgcgcct atcgtgtaag
     6361 aaagcccgcc tgctaccatc aaaataacac cagtcattcc aagagagtga taaagaggaa
     6421 cagcagccac gacacagagc caccccataa aaatatagat gatcgtagat acttttgata
     6481 ctctctcccg tttatgcaac gtcaacgctt tatatataat gccaagaatc gcaaacaacc
     6541 agattaatac aaataaagtc caacctagcc atcctcggat actcagcaaa caaaaaggag
     6601 tataacttcc tgcaatcaaa agaaaaatcg aactatgatc gaatacttgg aacacctttt
     6661 ttgcttttgt aaaaatcaat gaatgaaaca aagtagacgt taaaaataaa agaatcatca
     6721 tcgatccata gatagcataa gagacaatat ggagggagga atgcaagtgg gcacctttta
     6781 ctaaaagaac cactagtccc gcgatactca atccagtccc aattccatgg gtgactgcat
     6841 taaacacttc atttaatatt tcatacgttc gattaaattt cgttttttcc atgaaaacgg
     6901 ctcctttact aggtgtaatt aatgaaaaat cattatttgt ctgttatact aaaaatagaa
     6961 attgtcttac atgtagttta acatgaataa ggcatcttgt taaagaaaga gagagagaaa
     7021 ataatgacaa atgtaaaaat cgtaacagat tcttcatgta cgatggaaaa aaatgtacgc
     7081 gatgctttag gcattcgtgt catgccgcta tccatcatga tcgacggaac agtatatccc
     7141 gatgatgacc aactagacgg cgaaagattt atggaaatga tggcgaaagc aaaaaatctg
     7201 cccaaaacaa gccaaccacc aattggagaa ttcatctcga tgtatgatga gctaggagaa
     7261 gacggcagtg aagtgatttc gatccatatg acaaaaggct tgagtggaac agtagaagca
     7321 gcaagacaag ccagtaatct aacaaaaacg aaagtaacgg ttatcgacag tgataccact
     7381 gaccaaggat tatcttttca ggtaattcga gcagctgaat tagcaaaaga aggtaaaaca
     7441 gtagagaaaa tccttgaaga agtcaaaaaa gtacaagaca atacaaaatt atacatcgga
     7501 atatccacac tagataactt ggtaaaaggt ggaagaatca gccgaacaac tggtttgctg
     7561 tctagtgttt tgaacatacg cgtggtgatg aatttcgccc attctgaatt aatccctgta
     7621 actaaggggc gaggaaagaa aacgttcgat aaatggttta ataatttgaa aaatgaactg
     7681 aaagaattgc caaatgtgcg acaaatcggt atttcacatg cggatgcaaa agaattggcg
     7741 caagaattca aagagggttt gcaagaatta ttcccggaca tgcatatccc tgtactgcat
     7801 acgaatccgg ttatcgctac gcatactggt aaagacgcgt ttgcaatcat gtattatgta
     7861 gattagtcga aaagaggata agccctcagc ctgaaaggct tgcagcttat tctctttttc
     7921 tttataaaat taggaaaata attgttttct tttttcataa agactacctt tttgataaaa
     7981 tttttgataa cattagaacc aatcgaatac aagaggtgaa aaaatgaaat taacgaaacg
     8041 acatttgatc ctcgttttgt tcccattatt aattgcgatc atcatctatg caggtttagc
     8101 tgcttctata ccgaaagctg gcccactaat gaaaccagta actactgtga gagaagatcc
     8161 aaataaaaaa gaaaaacttc actacatagc tatcggagat tcgctgactg aaggagtagg
     8221 agatcaaacc aagcaaggag gattcgttcc ccttgtcgca aatgatctaa aagaccgtta
     8281 taatttgacc gcggttgaaa cagaaaacta tggtgtaaat ggtgaacgaa gtgaccagat
     8341 cttaaagcga gtaaaaacaa aagaagaaat tcgaaacaat atcaaaacag ctgattttat
     8401 cacactgact gttggcggta atgatttgat gaaagttatc caaaataatt tattcgggtt
     8461 aacggtcaat tcctttaaaa agccactgaa aaactatcag gaaaatgtca cagaactatt
     8521 ggaagaattt cgatccctca ataaaaccgc acctatttac attttaggca tctacaatcc
     8581 attttatttg aactttcctg agatcactga catgcagaca attgttaaca attggaacaa
     8641 aggaacagaa acggtgacaa aaaaaatgcc gaactgctac tttatcccaa tcaatgattt
     8701 gctttatcaa ggattgaatc aggaagtagg tgtcacagaa gaaggaacag atcaaacaac
     8761 tagcacgaca gaaaatctaa atggcatcaa aaataatgtg ctctatgaag aagatcattt
     8821 ccatccaaat aatttaggtt atcaattgat ggcaaatgca gtgagagatc agctgatcga
     8881 gacacaaaac caatggttag agaaggagga tgatcaataa acatgaaaac aagatctgac
     8941 gtatcttcaa acaaaacaaa gaaaacaaat tactggaaat tcgccttcct tatccttctt
     9001 ggggtagtac ttggttcgac cgtattttta ggcagccgta tttttgctaa tcgggaacca
     9061 gaactcccag aaataccagc tcttacggaa agacaaggag atcctgtctt aacgatcaat
     9121 tcaaataaag aaaaagtcaa tcaaatcatc agctttttcc ttagtgagta tcaaaaagat
     9181 agtgatattg aatataaatt ttatttggaa aatgaagcac tactcaatgg tactttcgaa
     9241 gtactaggtt ttcctatcga tttctattta tattttgatc cgtatgtgat ggaaaacgga
     9301 aatgtccaac taaaagctag aagtctttct atcggaacat tgagtttgcc aatcaaagat
     9361 gtgatgaata tgatcaagag aaactataaa ttaccagatt ggatcgaagt caatacagat
     9421 gatctgacta tcatgcttag gttggatcaa ttccgaatgc aaaacgggat gtatattaaa
     9481 gcagataaaa tcgacttagt aaatgatgac attcggttta gtctatattt gccaaaagat
     9541 acctctgatg cttcaaacaa ctcaaatacc aacaaaacta gcaccagcag tactgaaagc
     9601 aagacaaaag aaaataaaaa atcatcggat gagtagtatt agagggggaa tcataatgga
     9661 acgagcaata tttgcaggag gctgcttctg gtgtatgatc cagcctttcg acacgcttcc
     9721 tggtatccat acagtgatgt caggatatac tggagggcat gtaccagatc cgacttatga
     9781 acaagtaaaa agccaaacga ctggacatac agaagcagtg gagattatct atgatccaac
     9841 attgatttca tatgaacaat tagtagaact ttactggcag caaactgatc caactgacgc
     9901 tttcggacaa ttcgaggatc gcggtgacaa ttatcgcccg gtgatctttt atttgacaga
     9961 agaacagcgg atcattgctg aaaaaagtaa gaaacagtta gctgaaagtg gacgtttcga
    10021 acagccaatc gttacaaaaa tcgaaccagc ccaaccattt tatctagcag aagaagacca
    10081 tcaagactat tataaaaagc atcctgaaaa ttttaaaaga aaccatgcac gccgggctgc
    10141 ctttatcgaa gaaaattgga ggaacgaagg gtgaatcgaa gcttttttca ttatttaatg
    10201 acactgcgag gcggtggcac acatgatgct ttatctgttt tcgctacaga agccggtaaa
    10261 gacatccaat ttcctaagca cagctctagc tacgaagaaa tttctaatta tctggaactg
    10321 aatgtcgact atttgccgtc catggatatc tttgacgaag catgggaaaa atatttagaa
    10381 aataatcatg acatataaga gatagttttg tactttatga tcaaaaaagt tttttgtcca
    10441 atagcctaat tgaaagaatt atttttattt cagctatcta gtcgcatttt tttgatattt
    10501 ttcttataat aaagaaagaa tgaaaaaaag agggatggat tgatagagat tgacaaaaaa
    10561 acaacgctat attaatcgct tctggcttgt attcaaatac ttacttgttt ctgtattcct
    10621 tgcgttcatg ttgcggggat ttttattcat cccggtacca gtggaaggca attcaatgga
    10681 aaacgtgttg aagcaaggtg atatggttgt gatggaaaaa ttttcggaga tcagacgatt
    10741 tgatattgtt gtttttcaat tagcagatgg cacgatttat atcaaacgag taatcggtct
    10801 tccaggagaa aatgtcagct accaaaatga tcaattgaag atcaatggga aagtagttaa
    10861 ggaaccttat ctaactaaaa acctgaaatc agatcatgca aatgcatcct atacgacgga
    10921 ctttacacta caagagctaa cgggacaatc caagcttccg gaagataatt attttgtact
    10981 tggagataat cgaagagttt caaaagatag tcgttcgttt ggcacgatca ataaaactga
    11041 tattctaggt aaagcaagat ttgtctatta tccacttgat gaaataaaat ggatccagtg
    11101 agaaacaata aagcgtgcag tctgttgaga ctgcacgctt tattgtttaa tccaaaatca
    11161 gtttactacc ttagtacgta aaatcaaaca gctcgtttaa acacttttct agcctctaaa
    11221 gttttgataa atgaataaac aaccattatt tgaataggga tttggatcat attcctgatg
    11281 aatctaccta gaaaaattac tttgaaatct gcaccaaaca aaacagacat cagccaagaa
    11341 ttgataaaaa ggctgactgg gatcataatc aataattggg cagcgatgac ggaaggcagg
    11401 tttatctttt tatgatataa aaataagcca ttaataaatc caccaaggaa agccgtgata
    11461 agaaaagcag cttgaaacgt tcctgaacca aatatcgtga aatttaaagt gttcgacaac
    11521 atttcaagca aaccagccca gatcggtcca tatagataac ccataagcgc catacttaaa
    11581 aatccgaatg tgatttgaaa ataagcagga ccaatcgcta tcttgtataa gatgacatca
    11641 agggctaata aaagagcaga ggtcgacaac aatcttgcat tcatttcggg caatctaaaa
    11701 acgtcttttg caatcaaagt gttcaaaata ttctctcctt tgtcagttaa taataaaagg
    11761 ataccataaa atcaccaatg tgtcatgaca cattggtgat ttacatacac tggcatctaa
    11821 caattaaaaa aagggtgaga agactgcttt tataaactgt aaaaaagaga ttgtacgaat
    11881 aaaaaaaata gaggcggaga atacttatcg tatcatccgc ctttgttttt ttgttataaa
    11941 aaacgtcaga ttagttatct gcttacatct tcatccatac gggttctaat gtatttccgt
    12001 ctagatcttg gacttctaat gaaaacattt cttcttcagg cattcccata tctacttcgt
    12061 ggaaagtacc accattttct tttgcaattt gagcaaattt ttttacttca tccgcatttt
    12121 caacactaaa cgaaatcaaa gcaccacttg tttttttatt atcagctatt tctttatctt
    12181 ttataaattt tttataaaaa tcatgagaga gaagcattat ccaaaaagtg tcatcccata
    12241 ccattgaacc agcttcttca gtagaaaatt cttcattttt tttaaaaccc agcttttcat
    12301 aaaaagcaac ggaacgattg acatcagcaa caggaaaatt aacaaaaacc atactattca
    12361 tttgtttcgt ctcctttctg ttttgttcgt ctatcatctc ttatttagga aacgctgtca
    12421 aaaatttaga ttcatatata gaaaccaaaa taaaaatttg catcatcaaa aaagtgaaga
    12481 tgtgatagaa tgaggactgg ttcactttca cttaactaat agatggaatt tctttataat
    12541 aaaaaagtga atttatattg aaaaagaaaa aaacaagtaa agccggggaa tattccggta
    12601 tgaaaggaat ggagagaaca atgaccatcc aatggtttcc gggacatatg gcaaaagccc
    12661 gacgagaagt ttctgaaaaa atgaaatatg tggacatcgt atttgaattg gtagacgcac
    12721 gattaccttt atcttctcgt aatccaatgc tagatcagat cattcagcaa aaacctcgcc
    12781 ttgttttatt gaataaagcg gatctggcag atccacaaca gacaaagctg tggcaagaat
    12841 attttcgtaa acaaggccat cttgcattag ctatcacggc acaagaaagt aaaggaatta
    12901 aaggtctagt acctgcggct aaagaagcat tgaaagaaaa acgggcaaga gatgctgctc
    12961 gggggatcaa gcctcgcgct attcgggcaa tggtcttagg tattccaaat gttgggaaat
    13021 ctacgttgat gaatcgacta gtaggcaaga aaattgcaca gacaggaaat aagcctggcg
    13081 taacaaaagg tcagcagtgg cttcgtttag gtaatgagtt ggaattgtta gacacaccag
    13141 ggattttatg gcccaaattt gaagatgaag aaatcggcaa aaaactagcg ctgacaggcg
    13201 cgatcaaaga ccagcttgtc catttagatg atttagcgat ttacggatta gacttttttg
    13261 cccgatatta ttcaggaaga atcagcgatc gttatggttt atcaatcgag caagaagaaa
    13321 aattggcacc ggaattactg atggatatta cagcaaaacg tgggtataaa gaagactatg
    13381 atcgtggcag cgaattagtc gtgcatgata ttcgtcaagg aaaacttgga cgatacacac
    13441 tggatcgttg cgaagaaatg gagcaaaaaa gtgatgactg aatccatcca aagtatcaaa
    13501 gaacgattga aaagcataca gagaatagat gatcctacac tagaatcatt aagaaatgat
    13561 ccacgcaaag gcgtgcagca agcactggct tcttttgaac gacgattgaa aaaggaagca
    13621 ttaattaagg aaaaatatga agaaatgttt tgctttgaaa agcaagggta cgcacaaggt
    13681 caccaatgga ttgccggcat tgatgaagta ggaagaggac cattagctgg acctgtcgtg
    13741 gcagctgcag ttatcttgcc gaaaaatgag atcatattag ggctgaatga ctccaaacaa
    13801 ttatcagaga aaaagcgaga acttctttat caacaagttc aagaaaaagc cctggcaatc
    13861 gggatcggga tcgttgatca gcagacaatc gatgatgtga atatttatga agcaagtaaa
    13921 ttagcgatga tccaagcagt agaaaaattg tccattgtac caagctattt gttgatcgat
    13981 gcgatgacct tggatttgcc tattcagcag gaaaaaataa tcaaagggga tgcacgatct
    14041 gtgtcgattg ctgctgcaag tatcattgct aaagtatatc gagaccaatt gatgaaagaa
    14101 tatgacgtgc tttatcctgg atatggtttc tcgcataatg caggttatgg aacaaaggaa
    14161 cacttagaag gacttgaacg acaaggaatt acgccaatcc atcgtaaaag ttttgcacct
    14221 gtaaaaaaat atttacaata aaaaacagcc gcttttacgt atcttattag aaagagagac
    14281 gaaaggcggt tttatgatgt atcaaataga agaaaattta ttgaaacttg cttatacaaa
    14341 aggaatcagt agtcaaggaa aatgggcact tgctaactgg atgatgcagt atccttataa
    14401 agaaatcgga tttgaagaca tcattaagat aggtaaaata accaaacacc gggagctgtt
    14461 tcgcaaaagt tggcaggaaa ttcacgctaa ctggggcaaa atccaatcga aacagtcgtt
    14521 tatcacttgc tttgatccca aatatccccc tcagctgctt cacttgacct atccgccaat
    14581 tgtactgttt tataatggtg atctatcttt actttcttgt aacatgttaa gcgttgttgg
    14641 tggaagacac acttctgctt tagccaaaaa aacagtttat tatttactaa aaccagtgat
    14701 agaagctgga tatgtgatcg tcagtggctg cgcgaaaggg atagatacat acggtcacca
    14761 agcagcaatc tctcatggtg gacggacaat cgcagtcatt ggaacaggga tagagcaagt
    14821 ctatcctgct gaaaatcgtg acttgcaaga ggtgattgca aatgatcatc ttcttttgag
    14881 tgaatatcct cctcaatccg gcccgaaaaa gcattgtttt ccaatgagaa atcggattat
    14941 cgcagctttg tctaaaggaa catgtgtctt tgaagccaag aaaaaaagcg gctctctgat
    15001 aacagcacag caagcattgg atcttggaag aacagttttt tctgttccag gaaatatttt
    15061 aacagaacat tctaccggat gtcatcattt gatccaagac ggtgcagttt gcacggtttc
    15121 gggtgaagat atcttggcag aatttaaaga ataacaccat acttttcatt tgcttttctt
    15181 gacaaacgtt ttctgaatgg atatgattag ctacgatttg tatgaatcct cttttaatag
    15241 taggaacacg acaatggaaa ggagtccatt cagtaatggc gtataaatat ttagtgattg
    15301 tagaatcacc tgcaaaagcc aagacaattg agaaatatct tggacgaaac tataaagtaa
    15361 tggccagtgt tggccatata agagatttac cgaaaagtaa aatgggtatc gattttgaaa
    15421 acaattatga accccattat atttctatac gcggaaaagg cgatgtcatc aaaagcctga
    15481 aagccgcagc aaaaaaagct caaaaagttt acttggcaag tgacccggat agagaaggag
    15541 aagcgattgc ttggcattta gcgtacctac ttgggttgga tctgaaagaa aaaaatcggg
    15601 tggtcttcaa tgaaatcaca aaagacgcag tcaaagcagc ttttaaggaa ccaagaacga
    15661 tcgatgtaga tttagtagat gcacagcaag ctcgtcgtac cttagacaga atcgttggtt
    15721 attcgatcag tcctattctc tggcgtaagg tcaagaaagg gttaagtgca ggacgtgtcc
    15781 aatctgtcgc tttaaaaatt attattgacc gtgaaaaaga gatccgagaa tttgttccag
    15841 aagaatattg gagcatcgac ggtaatttta aaaaagctcg caagaaattc aaagcaaatt
    15901 tctggggaat cgacggtaag aaaaagaaat taccagatgc acaaagtgta aaagaagtca
    15961 ctgctagaat tgatggaaca gacttcgaag ttaaaaaagt agaaaagaaa gagcgcaaaa
    16021 gaaaccctgc gaatcctttc actacaagta gcctgcaaca agaagctgcc cgtaagttaa
    16081 actttcgaac aagaaaaacc atgatggtag cacagcaatt atatgaagga atcactttgg
    16141 gtaaacaagg aacagtcggc ttgattacat acatgcgtac tgattctacc cggattgccg
    16201 atacagcaaa agcagaggcc gcagcattta ttgaagatac gtatggaaaa gaattttcta
    16261 gacatgatca ccgaaagatc aaaaatgctc aaggcgcaca agatgcccat gaagctgtgc
    16321 gtccaacgag tgtgcttcgt acaccagatg aactcaaaca atatttagat aaagatcaat
    16381 tgaaacttta tacattgatc tggtcacgtt ttgtggctag tcagatgaca ccagctattt
    16441 tagatacaat gaaagtgaca ttagaacaaa atggtgtcat cttcattgcg aacggttcaa
    16501 aaatcaaatt caaaggattc atgcaagtgt atgtggaagg tagagatgac ggcaaagaag
    16561 agaaagaaaa tattcttcca gaacttgaag aaggcgatgt agtccaatct gttgatattg
    16621 aaccaaaaca acattttact caaccaccag cacgttttag tgaggctacg ttgattcgtg
    16681 cactagaaga aaacggggta gggcgacctt ccacttatgc accaacactt gatacgatcc
    16741 aaagacgcta ctatgtaaaa ttgacacaaa aaagatttga gccgactgaa ctaggagaaa
    16801 ttgtcaattc attaatctgt gaattcttcc ctcaaatcgt cgatatccat ttcacagcag
    16861 aaatggaagg agatttggat aaaattgaag aaggcacgga agcgtgggtc aaagtggttg
    16921 atcgtttcta taaaccgttt gaaaaagaac tgacgaatgc ggaagaaaaa atcgaaaaaa
    16981 ttcaaatcaa agatgaacca gcaggattcg attgtgatgt ctgtggacat ccgatggtga
    17041 taaaattagg gaaatatggg aagttttatg cttgcagtaa ttttcctgat tgtcggaata
    17101 cgaaaccaat cgtaaaagaa atcggtgtga cttgcccagt atgtcatgaa ggacaagtca
    17161 ttgaacgaaa atctaagaaa aacagactat tctatggttg ttcccgtttc ccagaatgtg
    17221 aattcacttc ttgggataaa ccagtgggca gaaattgtcc aaaatgtgac ggatatctgg
    17281 tcgagaagaa agtcaaaggc ggtaaacaag tagtctgtgt aaatggagat tacgaagaag
    17341 acatccaaaa ataatagaaa agaggttctt tcattgaaaa tggtaaatgt tgttggagca
    17401 gggcttgccg gaagtgaagc tgcttggcag atcgcacaag caggagtccc tgtccgttta
    17461 tatgaaatgc gtccagtcaa atcgactgag gcgcatcaca caagtaattt tgctgaacta
    17521 gtatgttcca attcattaag aggaaacagt ttggctaatg cagtagggtt attgaaagaa
    17581 gaaatgcgcc gattgaattc ggtagtaatc acttcggcag atgaaacagc agtgccagct
    17641 ggtggtgcct tagctgttga ccgcgattct ttttctgaaa caattacccg aaaagtcaaa
    17701 gagcatccat tagtaacagt gataaacgaa gaaattactg aaataccgga aggaatcacc
    17761 gtagtagcaa caggaccttt gacttctcat ccattagcag aaagcatcaa agcattcaat
    17821 ggttcagatg gtttttattt ctatgatgca gctgcaccaa tcgtagacaa agctacgatc
    17881 gatatggata aagtatattt gaagtcccgc tatgacaaag gagaagcagc ttacttgaac
    17941 tgtccgatga cagaagagga attcaaagct ttccatgagg cactaatcaa tgccgaagtg
    18001 gcagaactta aatcttttga gaaagaaaaa tattttgaag gatgtatgcc aattgaagta
    18061 atggcacagc gaggattcaa aacgatgctt ttcggaccta tgaaaccagt tggattggaa
    18121 gatcctaaaa ctggcaaacg tccttatgct gttatccagc tgcgtcaaga caatgctgca
    18181 gcttctttat acaacatcgt gggctttcag actcacttga aatggggcga gcaaaaacgt
    18241 gttttccgga tgattcctgg gttagaaaat gctgaattcg ttcgctatgg tgtgatgcac
    18301 cgaaacagct ttatgaattc gccagaattg ctgaaaccga cttaccaatc aacgaaacgc
    18361 gaagatctgt tctttgcagg tcagatgact ggtgtagaag gctatgttga aagtgcagcg
    18421 agcggactac ttgctggaat caacgccgca cgcttagcaa aagaaatgga accagtcgag
    18481 tttccacaag aaacagctat tggaagtatg gcttactaca ttactcatgc ggaaggaaaa
    18541 catttccaac cgatgaatgc caactatggt ctgtttcctg aactgccaga gcgcattcga
    18601 gacaaaaaag caagatatga agcaattgca caacgagctt taaaagctaa tgaggcaata
    18661 aaaacagaat tagaaaaaac tgcaatccca aatgagtaag gaaactccat gaagagggtg
    18721 agacggatag tctcgctctt tttcttgcgt ttttcttaag aatttctcaa acaaaatgtg
    18781 aggaattgcg ccaattgtga acaaacttag aaatattggt taaattctga caatttattt
    18841 gtctttttat aaacgctgtg ctaaattatt ctctgggaca aaaggaaggt gagaaaatga
    18901 cagaagccac gtgggaacaa aaatttttgc gttatttgat tgtcgaaaga ggatattcag
    18961 aaaaaacgaa agaagcatat gaagaagaca ttctccattt caaacatttt ttggaggaat
    19021 ctggagacgc tgatctgctg aaggcggaat attttacggt tcgtgtctat ttgagtgcat
    19081 tagctgatca tcgtcctagc tatagcagaa attctatcag tcgcaagata gccagcctac
    19141 gttcgtttta tcagtatctt ctcaaagaag aagtgataaa agaaaatcca ttttcttacg
    19201 ttcatctcaa aaagaaaaat ttgagactgc cacgtttttt ttatgaaaat gaaatgcaag
    19261 cactttttga cagcgtagca ggagatagtc ctttggattt gaggaaccgc gctttactgg
    19321 aagtccttta cgggtcaggc atccgtttaa gtgagtgtag cagtttgtcg gtatcagaca
    19381 ttgattttga ttcagaggtc atgttgatcc atggtaaagg gaacaaagag agatatgctc
    19441 ctctaggttc ttttgctcaa gatgctctgc aagaatattt cagggaaggc cgccaggtat
    19501 tgatgacaaa atatcacgaa gagcatgata tcgtttttgt gaatcatcat ggaaaaccaa
    19561 tcacgcctac tggaattgaa tatgtcttga atcaaatgat caaaaaaagc agtttggata
    19621 gtgatatcca tcctcacatg ctgcgccata catttgctac tcatttatta aataatgggg
    19681 cagatttgcg gacagtccaa gaattgctag gacatgccaa tttatcgact acccagatct
    19741 atgcccatgt aacaaaagaa agtttacaaa aaaactatcg ttctttccat ccaagagcgt
    19801 aaaaataagg aaaagaacaa atttaggagg aaacaaacat ggtcgaatca ccatttcatt
    19861 caaccactat ctgtgccgtc gaaaaagacg ggaaatttgc tatggctgga gatggccaag
    19921 taacaatggg tgaatctgtt gtcatgaaag gcactgcaaa gaaagtacga cgtatctaca
    19981 atggagaagt tgttgtaggt ttcgccggaa gtgttgcaga tgcgtttact ttagaagaaa
    20041 aatttgaagg caaactaaac gaatataacg ggaatctaca acgcgcagct gtcgaattag
    20101 ctcaagaatg gcggacacag caatctatgc aaaaacttga agcgatgctg atcgtaatga
    20161 atgataaaga aatgcttctt gtttcaggta caggagaagt tatcacacca gatgatggta
    20221 tcttagctat cggatcagga gggaattttg cgttggctgc agcccgagca atgaaaaact
    20281 atgctcaaaa cgacatgagt gcgaaagaaa ttgcagaaaa tgcgttaaat attgccgcag
    20341 atatctgtat ctttactaat cataatatta tcgtagaaga actgtaagag gtgagtagat
    20401 atgtatgaat taaaacaaac accaagacaa atcgtaagtg aattagatga atatatcgta
    20461 ggacagcacg cagcaaaaaa atctgttgcc gttgctttgc gtaaccgcta tcgccgtttg
    20521 cagttagatg aaaaaatgca acaagatatt acaccaaaaa atatgctgat gatcggacct
    20581 acaggggtcg gtaaaacaga aatcgcacga cgtttggcaa aaatcgtcaa tgcaccattt
    20641 gtcaaagtcg aagcaacaaa atttactgaa gtcggttatg tcggaagaga tgtagaatca
    20701 atggttcggg atttagtaga aaatgcgatc caaatcgtag aaaaagagca gtacagccgt
    20761 gtatacacaa gtgctaaaaa aaatgcagaa aaacgtttgg taaaaatctt ggtacctggt
    20821 atcaaaaaag aacagaaaca atccactaat caatttgaac aaatgatgaa tctttttaat
    20881 gctagtcagc aaaataccgc tgaaccgcaa gaagaagtga cagaagatat tcgaatcaat
    20941 cgccgtacag tcttggaaca actacaaaag ggtttactgg atagtcgtga agtaaccatt
    21001 gaagttgaag accaaaagaa acctgcacca atgatgaaca atggattgga gcaaatgggg
    21061 atcgacctaa atgaaactct aggtgctttg acaccgaaga aaaaagtgga acggacggta
    21121 acagtcaaag aagctttaga gttattgatc aaggaagaat cagcaaaatt agtcaaggat
    21181 gctgatatcc atagtgaggc aatcaaatta gcggaagaaa gtggaattat ttttatcgat
    21241 gaaatcgata agatcacttc taaatcacag caatctggag aagtatccag agaaggcgtc
    21301 caaagagaca tattgccaat tgtcgaaggt tcacaagtaa acacaaaata cgggatcatc
    21361 caaacagatc acgtcctatt cattgcttca ggagctttcc atttgtctaa accaagtgat
    21421 ctaattccag aacttcaagg acgcttcccg atacgggtag aattagatga tttgacagct
    21481 gaagattttg tgaagattct gaccgaacca aataatgctt tagtaaaaca atacgtcgcg
    21541 ttgatcggta cagaaaatgt gtcagttacg tttacaaaag aagcaattga acggatcgct
    21601 caaatcgctt tcgatgtcaa ccgtgacaca gataatatcg gcgcacgcag actgcatacc
    21661 attttggaaa aactactaga agatctatta tttgaagcac cagatatgca aatgggagaa
    21721 ataacgatca cagaagcata tgtggatgac aaacttggct caattgcggc aaatgaagat
    21781 ttaagtcgct atattttata aaaaatcgga ggacgacaat gacagacttg ctaacgaaaa
    21841 cccgcaagat aaacacactt ttgcagcaaa agaatacttt tgacatgcaa gcagatttgc
    21901 cttacgataa aatggctgtc acactaggtg gtattcttga cagcaacacc tatatcatag
    21961 gaagtgacgg agtattacta ggttataatg aacgtcacga tgtgaacaat gaacgaatca
    22021 aaacaatgtt tgttgagaaa caatttcctg aatcttatac tgatacagtg gcacagttga
    22081 tgaaaactga agctaatatc ccaattacta gtgatattac tgcatttccg gtagagtcaa
    22141 gagagaaata tccatttggt ctgacgactg tcgttccgat ctttggagct ggcgaacgtt
    22201 tgggtacgat tatcttatct cgtatggaac aatcgtttgc agatgacgat ctagtcttag
    22261 cagaatatgg agctacggtt gtcggtatgc agatccttta tcagaagtct cgaagcatcg
    22321 aagcagatgt acggagtgca acggctgtcc aaatggcaat caacacatta tcttatagcg
    22381 aattgaaggc tgtacaagct atctttgaag cattggatgg agatgaaggt cgtctgactg
    22441 cttctaatat tgccgatagt atcggcatca ctcgttctgt gatcgtcaat gctttacgaa
    22501 aattggaatc agctggaatc attgaatctc gttcgttagg catgaaggga acttatttaa
    22561 aagtattgaa ttcccgtttt aaagacgagt tagcaaaaca cagttattag aagtaagggg
    22621 actggggcag atagaaatgt ttgttccagc cttcccattt taaaaagtaa gtcgaagttg
    22681 gaggaatggc aatgactgtt attattcaaa atgaccagct gatcgccgaa atcgctgaac
    22741 acggtgcgga attgatcagt ctgaaatcaa aagaacatgg tttagagtat atctggcaag
    22801 gtgatccagc gtattggggc agacatgcac cggttttatt cccttttgtt ggacgtttaa
    22861 aagatgatca gtatgtttat caagggaaaa catatcaaat ggggcaacac ggatttgctc
    22921 gggatatgga ttttgaagtc attgaacatg gacaagaaat ggctagtttc atcttaagga
    22981 gtacgccgga gacattagaa aagtatccat ttgattttga attagtgatc agctatgaat
    23041 taggaggaga cggcatcacg gttcattatc aagtggcaaa taccggtgaa aaagaaatgt
    23101 tcttttctat cggtggacat cctgctttca atgtacctct tgaagaagga ttgtcatttg
    23161 aagatttcta tctggcattt tctccattga aatcaagatt acgcctacca ttgaaaggcc
    23221 cattcatcga tatggatcaa aagacgttag gtcagacaaa tacgaatctg gctttgaatc
    23281 gtgaactttt tgagcaagat gccttggttt ttgaaacaag gggtctaaat gcgtacgcca
    23341 ttcgaagtga aaaatcttcc catagcgtaa ctttaagcta taaagatatg ccttatgtag
    23401 ggatctggtc tccttatcca aaagaagcgc ctttcgtttg tatcgaaccg tggtgcggaa
    23461 tcgctgacac ggtgaacagt agtggaaact tgatggaaaa agtagggatc aatcaactag
    23521 gtgcccatga aatcttcaag acaaaatatt ccatcacagt gaagtaagag gttgtgaccg
    23581 agaagttagc agggaacacc gtttaccaag gtgtgtaaaa aagctatcct gtaagtaata
    23641 agggagtgtg acatcgattt gtcacactcc cgttttatct ctttggaatg aaaaagtatc
    23701 aggatttttt cttgctgcta ttcaaaccaa acgggattcg actttcagta tggtttctga
    23761 tacgcttgat attgtcttta tgccgataaa agataaagga agaaactaaa aaggcaatga
    23821 tcgtcagtaa ccaattgggc ttagccaaaa tcacaggagc tacataagga aggacgatcg
    23881 tagataaagt gatcaatact gcactgatca tactgctcaa actcaccata cttgtacaaa
    23941 ataaactaac gataaagatg atacaagaat aaacaaaaaa tacaggatta aatcctaaaa
    24001 gcattcctgc acttgtcgca acagcttttc ctcctttgaa cccagcaaaa atcggaaatg
    24061 tatggccaag aatggcgcat accccaaacc aaagagggtt aatcccagaa aggtggaaga
    24121 tcatcggcaa acaagtagcc aatgtacctt ttaaaatatc cataagcagg acaacaatcc
    24181 cggctgtttt gcctaataca cgaaatgtat tggtcgttcc agtattaccg ctcccgtgtt
    24241 gtcggatatc ttttttgaaa aacaatttgc caatccagac accggaagga atagaaccta
    24301 aaagatatgc taaaataagt aataagatca atttcatttt ttaccctctt tcaagttgaa
    24361 aactatcctt tattttagca tgaataggta acaggaacaa ttgaaaaaat gaaagataca
    24421 agtataatag atggaataaa aagaaaagca aggggtggca gaaatgtctg taaaaaatcc
    24481 aacacaagaa acgattttca actatttgga agaagcaaaa aatattgccg tcgtaggctt
    24541 gagcaataaa ccagaacgaa caagttacat ggttgcacag gtattgcaag acaatggata
    24601 taaaatcata ccagtaaatc cattattggc tggtgaagaa attctaggcg agaaagtcta
    24661 tgcgaatttg acagaaatcg atgatccaat cgatatcgta gatgtattta gacggagtga
    24721 atttttacct gaaatagcag gtgatttttt gaaaactgat gctaaagtgt tctgggcaca
    24781 attagggatt gaaaacaaag aagcagctga gatgctaaga aaagcaggac gtacagatat
    24841 cgtaatggat cgttgtatta agattgaact tggtaagttg aaagaaaacc aaaaataaaa
    24901 atgaccacaa agaagagatg atcgatagag gggaatgtcg aaaaagcggt taggagataa
    24961 ttaaaattga tttctcgtct accctctttt ttttcacgac cttttctagt atgataatcg
    25021 tggtgcggtt ttgccgcaaa gtattattga aggagcgttt gccttggcaa aaaaagtaaa
    25081 caacgaatac aatgactcgt ccatccaagt attggaagga ttagaggcag ttagaaaaag
    25141 accagggatg tacattggat ccactgacag ccgaggcttg catcatttag tctatgaaat
    25201 cgtcgataat gcagttgacg aagcattatc aggttatggc agtgagattg atgtgaccat
    25261 ccatgaagac aatagtataa ctgttgcaga tagcggacgt gggatgcctg taggaatgca
    25321 tgcttccgga ataccaacag ttgaagtgat ttttactgtg ctgcatgctg gcggaaagtt
    25381 tggtcaagga gggtacaaaa cttctggtgg actgcacggt gtaggagcca gtgtggtaaa
    25441 tgccttatcg aaatggctaa ttgtaacgat cgttagagat ggcgtagagt atcagcagaa
    25501 atttaaaaac ggcggtaaac ctgacggtac tttgaaaaag ataggaaaaa caaaaaaagc
    25561 aaacgggacg actgtccatt ttttaccaga tgatacaata ttcagtacaa cgaaattttc
    25621 atatgaaatt ttagccgaac ggttaagaga atctgccttt cttctaaaag gcgttaaaat
    25681 ctctctttct gatcttcgtg gagaagaacc agtcaaggaa atttttcatt acgaagaagg
    25741 aatcaaagaa tttgttgatt atctgaatga agaaaaagat acattgacac cagttgttta
    25801 tttttcagga gaaaaagaag gtatcgaagt agaggtcgct tatcaataca atgacggtta
    25861 ttcagaaaat gttttatctt ttgtcaataa cgttcggaca aaagatgggg gaacccatga
    25921 agctggtatg aaagcggcga tgacgaaatc ttataatgaa tatgcgagaa aagttggttt
    25981 gctgaaagaa cgggacaaga atctggaagg tagtgatttc agagaagggt tagctgctgt
    26041 actttctatc cgtgtacctg aaaatcttct gcaatttgaa gggcaaacaa aagaaaaact
    26101 tggtacgcca gttgctcgta ccgtagtaga taacgtcatc agtgaacaaa tgggctttta
    26161 tttgcaggaa aatagcgaaa tgagccagat gttggtccgt aaagcaatca aagccagaga
    26221 agcgagagaa gcagcacgta aagccagaga agaaagccga aatgggaaga agcgtaaaaa
    26281 aggcgaatct cttctttcag gaaaattgac acccgcacaa tcccgaaacc ctaagaaaaa
    26341 tgaattgtat ttagtcgaag gggattctgc tggcggatct gctaaacaag gaagagacag
    26401 aaagttccaa gcaattctac ctttacgagg aaaagtcatc aataccgaaa aagctaaaat
    26461 gcaagatatc ttgaaaaatg aagaaatcaa tacgatgatc tatacgattg gtgcaggagt
    26521 cggtcctgag ttttctatcg aagactgtaa ttacgacaaa gtaatcatca tgaccgatgc
    26581 ggatacagac ggcgcccata ttcaagtatt actgcttact ttcttttatc gttatatgaa
    26641 accgctgatt gaagctggga aagtatatat cgcgttaccg cctttatata aagtatccaa
    26701 aggacaagga aagaagcaag tcattgaata tgcgtggaca gatgacgaat tggcagcaat
    26761 gattaaaaaa gtcggcaaag gatatatgct gcagcgatat aaaggacttg gagagatgaa
    26821 tgcggaacaa ttgtgggaaa caacgatgga tccgacttcc cgtacgttga tccgcgtccg
    26881 tatcgatgat gctgcgcaag ctgaacgtcg agtaaccaca ctgatgggtg acaaagtaga
    26941 acctcgaaga aaatggatcg aaaatcacgt gcaatttacg ctcgaagaag atggcagtat
    27001 cttagataaa aaagaggata ctgaaatctc tccttcagtt tctaatgatt tattagatga
    27061 agaacgagca gataaaaatg aaaacaatca gttatttgaa gtagaatagg aggtcacctg
    27121 atggaaaatc gacaagaagt acaagaatta actttagaag aagtgatggg tgaccgcttc
    27181 ggacgttatt ccaaatatat tatccaagaa cgtgcactgc ctgatatccg tgatggattg
    27241 aagccagtgc aacgccggat attatttgcc atgaacaaag atggcaatac gtatgacaaa
    27301 ggattccgta aatcagcaaa atctgtcgga aatatcatgg ggaattatca ccctcatggt
    27361 gaccgcagta tttatgaagc gatggtccgc atgagccaag actggaagtt gcgggaagta
    27421 ctgatcgaaa tgcacgggaa taacgggagt atggacggtg atccacctgc tgcgatgcgt
    27481 tatactgaag ccagattatc caagttaagt ggagagatgc tagcagatat cgagaaagaa
    27541 acagtcgatc tcgtgtggaa ttttgatgat acagaaaaag aaccaacagt ccttccagct
    27601 cgatacccaa accttctcgt caatggatcg acaggtatat ctgctggata tgcaacagaa
    27661 atcccgactc ataacttagc tgaagtgatc gatggcacaa tctatatgat cgatcatcca
    27721 caagcgagcc ttgaaaaatt gatggaatat attcctggac ctgactttcc aacaggaggg
    27781 atcttgcaag gaaaagatga aatcaaaaaa gcttatgaaa caggacgagg aaaagttatt
    27841 cttcgttcga aaacaaagat agaggcaatg aagggtaata aacagcaaat cgtgatcaca
    27901 gaaatcccat atgaagtaaa taaagcgacc ttggtgaaaa aaatggatga gatccgtttg
    27961 aataagaaaa tcgatggaat agctgaagta cgagatgaaa gtgatagaac aggtctgcaa
    28021 attgtcgtcg aattgaaaaa agacgccaat gctcaaggaa ttttgaatta tttgttcaaa
    28081 aacactgagc tacagatcaa ttacaatttc aatatggtcg ctatcgacca tatgactccg
    28141 catcaagttg gactgaaaga cattttaaga agttatatcg agcatcgaaa gcaagtcata
    28201 acaaaacgta gtcaattcga tttagccaaa gctcaaaaaa gacagcatat cgtagaagga
    28261 ttgatgaaag ctttatcgat tctggatgaa gtaatcgcaa cgattcgtga aagtaaagac
    28321 aaaaaagacg ccaaaaagaa tttagtcgac gtctttcaat ttacagaaga acaagcagaa
    28381 gcaattgtca cattgcaatt ataccgtttg acgaacacgg atatcacaga gcttcaaaag
    28441 gaatccgaaa gcttgattgc gcaaatcaca gagttgaata aaatcctttc aaatgacaaa
    28501 gagcttttct ctgttatgaa aaaagaattg cgagaagtga aaaaaaatta ttcttccgct
    28561 cgtctaacga caatcgaaga cgaaatcgaa gaaatcaaaa ttgatacgca agtcttagtg
    28621 gcacaagaag acgtaattgt cagtgtgacg cgagaaggct atgtgaagcg aacaagcctt
    28681 cgttcttata gtgcttccaa accagaagaa atcgggatgc gagaaggcga ctacttgcta
    28741 tactcaggtg aattgagtac attggaccat gttcttctga tcacaaataa agcaaatgtg
    28801 atctaccgcc ctgtccatga actacccgat ttgaaatgga aagatgctgg tgagcacatt
    28861 tctcaaacaa tctccaatct gtctgtagac gaatcgattt tagctgtttt cccatatcaa
    28921 aaaattcaag cagagaaaac atttgtattc atcagcaaga atggattgat caagcaaaca
    28981 cgaatgacag agttcgaacc atggcgcacc tataagagcc gtccgctcag ctgtatgaag
    29041 ctaaaggcgg aagaagatga actgattgct gtgtatctga aagaagatca gccaggctta
    29101 gatgttttct tagtaacgca tcaaggaatg ggtttgcgct atcctttaac agatgttccc
    29161 gttgttggag caaaggcagc tggagtgaag tctatgaatt tgaaagatgg agataggatt
    29221 atcaacgggc tgcttgtgtt agcagaaggt gatacgccga tcgtaatcgt tactcagcga
    29281 ggagctgtta aacgcatgct tgctcaagaa atcagccaaa cttcacgtgc gaagcgggga
    29341 gtgacggtca tgcgtgagct gaaaaaaaat ccgcaccgta ttatctatat gtcggaagga
    29401 agatcaaaag aaataacctt gatgaatcaa aaagggcagc agttaacgat tgacccaact
    29461 gatttcccta taggtgatcg aacatcgaat ggatcatttg cgatggatga gaaaaaaggt
    29521 ggagaagtca tcgaagttat cgatgctcct gagattttca tcgagtcgta actgttgtag
    29581 tattttaatg aagagacgcc atctgtatta atacaggtgg cgtctctttt tcattcattt
    29641 ttaataaatc cttatacgtt tgcaaaaaaa gatgaaagtg ttcacaatgc ttaaatgttg
    29701 gtatgtcaac gttgatttaa atatatgtaa ataaaacata aaaattgtta aataaaaaac
    29761 aagaaaaccc ttgcaaagat agggtgctgt gttatactaa tcacgtaaaa actgttacat
    29821 aaaacagaaa tttaggaggt ccatacaatg gagcaatgga atggatttaa aggtaccaaa
    29881 tggaaagaag gcgtagacac tagagacttt attcaaaata actacactga atatcgcggt
    29941 gatgacagct ttttggaagg cccaacagaa gcaacagaaa aattatggga tgaattgcaa
    30001 aagttaaatg atatccaatt cgagcgtaac ggtgtctatg atatggacac agaaatcgta
    30061 tcaactatta cttcacacga tgcaggctat ttggataaag atttggaaaa aatcgtcggt
    30121 ctacagacag acaaaccttt gaaacaagca ttcatgccgt tcggcggaat caatatggct
    30181 aacaatgcgc taaaaagcaa tggctataca ccaaacgatg aattgacaca tattttcact
    30241 gactggcgca aaacacataa tcaaggtgta tttgacgctt atactgctga aatgcgtgct
    30301 gctcgtaaaa acaaaatcat tacagggctt ccagacgctt acggtcgcgg acgtatcatc
    30361 ggtgactatc gtcgtgtggc attgtatgga atcgactatt tgatggaaca aaagaaaaaa
    30421 gatcatgata tgactggtta tcatacaatg agtgaagaag tgattcgttt acgtgaagaa
    30481 atcactgaac aatatcgtgc attagcgcaa atgaaagaaa tggctgcgaa atatggctat
    30541 gatatttcta aaccagctgc aaatgcaaaa gaagctgtcc aatggctata cttcggatat
    30601 ttagcagcaa tcaaatcaca aaatggtgct gcgatgtcaa tcggacgtat ctcagcattc
    30661 cttgatatct atatccaacg tgatttggat aatggagtaa tcacagaaaa agaagcacaa
    30721 gaattgatcg accacttagt aatgaaatta cgtatggtca aattcgcgcg tacacctgaa
    30781 tataaccaat tattctcagg attccctatc tgggctactt tatccattgc aggtatggga
    30841 ttagatggtc gttcattagt tacaaagaat gatttccgtc tgcttcatac attaacaaat
    30901 atgggacctt caccagaacc aaacttgact gttttgtatt ctgaacacct tccagaagga
    30961 ttcagaacat atgctagcag aatcgctatc gaaagttctt cgattcaatt cgaaaatgat
    31021 aatcagcttc gtgaacaatg gggttctgat gattgtgcga ttgcttgctg tgtatcagga
    31081 acagttgtcg gaaaagacat gcaattcttt ggcgctcgtg ctaacttggc taaaacacta
    31141 ttatatgcca tcaatggcgg agtcgatgaa gtgactggcg cccaagttgg accagaatat
    31201 cgtccaatca catcagaaat actagacttt gaagaagtga aacataactt catgaatatg
    31261 atggattggt tagcagaact atatgtcaat acgttgaaca tcattcatta tatgcatgat
    31321 aaatattctt atgaagcagc tcagctggct ttgatggatt ctgacttgca acgcacattt
    31381 gctacaggta ttgctgggat ctctcatgcg gcagacagct tgtcagcgat caaatatgca
    31441 aaagtaaaag caatccgtaa tgaaaaagga atagcagttg attttgaagt ggatggcgac
    31501 ttccctaaat acggaaatga tgatgaccgt gcagatgaat tagtttcatg gatcttggaa
    31561 tacttcatgg gacaaatcaa acgtcataaa acttaccgca actcaaaacc aacaacttca
    31621 ttattgacga ttacttcaaa tgttgtttac ggtaaaaata caggaaatac tcctgatggc
    31681 cgtgaagctg gaaaaccact tgcgccagga gcaaatccaa gttacaatgc ggaacaaagt
    31741 gggttattag ctagcctgaa ctcaacagct aaaatgaatt acggctgtgc attggatgga
    31801 atttcaaata cccaaacaat caatccttca ggtcttggaa aagaacaaga tacaagaatc
    31861 gaaaacttgc gtaatgtctt agatggatac ttctcaaaag gagcttatca tttgaatgtc
    31921 aatgtattca gtaatgattt attgcgtgat gcaatggaaa atccaatgaa atatccgaac
    31981 ttgacgatcc gggtatctgg ttatgcagtt aaattccgtg acttgacaag agaacaacaa
    32041 ttagatgtct tgatgagaac ttcacacgac agaatgtaaa aagttcgtga aaaaagtttt
    32101 tcgacttagc gtcgaaagga cgttgaatag ccatcgcatc aaaatcgaat aatcagtatg
    32161 cttatttgaa agaaacgatt tacaggggca ggacttgtat gttccgtccc tgtaaatttt
    32221 acacataacg aagcggcaat tgcgaaaaat tttgttcaga aagcttcgct tcgacagcta
    32281 acttgtactt aaaatgaaat caaagaaaag atgtgaactt aaatggaaga aaaaacaatc
    32341 ggctatgttc attctatcga aactttcggt tcagtagacg gacctggact gcgctttgtc
    32401 gtgtttatgc aaggatgccg catgcgttgc caattttgtc acaatccaga tacttggaat
    32461 attggcggag ggaaagaata cacagcagat gaacttttag ataaagcaga acgcttcaga
    32521 ccttactggg gagataaagg tggtattact gttagtggtg gagaacctct attgcagatc
    32581 gattttttga tcgaactatt taaaaaagca aaagagcgga agatgcatac tacattggat
    32641 acatgcggaa aaccttttac gtatgaagaa cctttctttt cacgttttca agaattgatg
    32701 aagtatacag atttactgtt atttgacatc aaacacatcg ataatgaaga gcataaaaaa
    32761 ctaacgcatt gggacaatga aaatatttta gaaatggctc aatatttgtc aaaaatcaac
    32821 aaaccggttt ggattcgcca tgtactagtt cctgagcgaa gtgattatga tgaatatttg
    32881 atccgactag acaactttat tcaaaccttg tcaaatgtag acagagtaga gattcttcct
    32941 tatcatacga tgggaaaata caagtgggaa acacttggtt tgaaatatcc attagaaggg
    33001 atcgaaccac ctacaaaaga acgtgtcgaa aatgctaaac gattgcttca tacagaagag
    33061 tatacaaaat atttaacgcg ataggtcgca tcggaaagaa gataagtatt gcttctttcc
    33121 gtttttttgc tattattaaa taggagaaat cacaaaggag cgaatgacat gtcaaaaatt
    33181 ttagtttttg gtcaccaaaa tccagataca gatgcaatcg gggcagccat tgcgttttca
    33241 cacttgcaaa atgaattagg taaagaaaca gaagcagttg ctctaggtac acctaatgaa
    33301 gaaacacaat acgcattaga ccactttcag ctatctgcac cacgagtagt agagtcagct
    33361 aaagcagaaa atgcagatgt catgcttgtg gatcataatg aattccaaca aagtatttca
    33421 gatatcagcg aagtaaatat tttagctgta gtagaccacc atcgtattgc aaactttgaa
    33481 acagctaatc cattgtacta tcgtgcagaa ccagttggat gtacaagtac gattatttta
    33541 aaactatata aagaaaacca agtaacaatt cctaaagaaa tcgcaggaat catgctctca
    33601 gctatcattt cagatacatt gttgttcaaa tcaccaacat gtacgcctga agatgaagca
    33661 gctgcaaaag aacttgcagc aatcgctgaa gtcgatctaa acacttatgg acttgaaatg
    33721 ttgaaagcag gaacaaatct aagtgataaa tcagctgcgg ttcttcttga tttggatgca
    33781 aaaagtttcc caatgggcag ttccaacgtt cgtatcgctc aagtcaatac agtggacctt
    33841 aatgaagtga tggatcgaca agcagagcta gaagcagcaa tggaagatga aatgaaagca
    33901 aatggctacg atctgttcgt attagttgtc acaaacatct tagacagtga ttcagaatta
    33961 cttgttgctg gtaaaggaaa agaaaaagta gaagaagctt tccaaacaac tttgaccaac
    34021 aaccgtgctt tgcttaaagg tgtcgtttca cgtaaaaaac aagtcgtacc tcaattgacg
    34081 gctgtattta gctaatcaaa tggtagtatg aagcattcaa aatgaatgga taagcgattg
    34141 atataaaaag aaacatagaa gatactaaag gccgaagaaa tggaggagaa taacagtcaa
    34201 ttatgactct tgttcttcct cctttttacg taaaagcctt ttaagtaaaa taaaacggat
    34261 agaaaagaga acatatatga actattattc aaaaaataag gaaaactttt atcaaaaact
    34321 gaccgatgat ccgctttttt cttcattgac cgattatcta tatgaacatc gagaacaaga
    34381 aacgattcta agagaattga aaaaagaatt ttcgcaaaat aaattttccc attttcttga
    34441 tttattgatc gatgcaggat taatcaagag ggaagaacga agatatcatt tgaactttcc
    34501 aatatttgat cctaaagatt acttgcaaca agccacttcg gctgctgaaa ccatagctga
    34561 acaattgaag agactctctg ttgatgaaca aaaattagcg atgggagaag ttatttgggc
    34621 gtattgtttc gaagatgaac ggaaagaagc gtatttttat ggagtaagaa acagccgaga
    34681 gacagaattg ctgaggacaa ctgcaggaaa tgagaagtac cgttttatca cactttcctc
    34741 tatagaacac ttcccgctta ctttggctaa ttactttttt gtacagaaga atcagcttcc
    34801 tgtaacgaag gcattcaaag aattagccga acttattgga gacgtaaatg aatcgtactt
    34861 ttttgatcaa atagaagtta tcgttgacag gatacgtaag aataaatata aaaatcgccg
    34921 gcctagtatt ttccatcagt ctctacttgt gacgaatact ataaaggaag aagaatcatt
    34981 cacattagaa ttacctattg tcgaaaaaaa caattttgag attgaattac caacattgga
    35041 tccttcattg acaatggagg aaatggcctt tcttaaacga caaatctttt ctgagctgag
    35101 caagaaattt ataccgcatg cattttctta tatcaaagaa tatggaacag ttttagtgag
    35161 taaaacctaa gagagcagta agcaaaaatt atactgactc ttgcaggagg agcctttctc
    35221 ttcctttatg gttaaaagaa ttatttgagt attttgccta tctaccacct attagattca
    35281 ctacatggtc tggaaacaaa ggaatgcaaa aaaatggtga gaaatgtgga atgacaaagg
    35341 aagtagtgat aagaaaagtt agatttttaa ataatcaata ttatgattca gtcaagtatg
    35401 gcattttatg ggaagaacta gctgattaga ttaggaagtc catttgataa tccagaatca
    35461 ctaagaaacg aatggcgagt cattaataat ttatccaatc ttgggttatt cttttagatt
    35521 gaagtgttta aaactaaaaa agcatttatg aagtcgtaat agtcaggctt caaaatgctt
    35581 tttattttgt ttgttttttc tttgccacat aaatacacct agactgacga tgaagagcat
    35641 atccaacaga tttcctgcga cttggatcgt cgaagggttg gtcagtggca ttaaagcaag
    35701 gacaaaaaag gaaaacaagc tgtaaataaa acaggtgaca ggaactaatc gaaagaagag
    35761 cggacatttc aattggctaa aaatgagtga aaaaataaaa taagtaaaaa tggataaaat
    35821 cacgactaaa ccattcatga taaagaagaa aagcgtattg tgattaaacg aataaaaaag
    35881 aattgctaaa aaagaaaacg ttttaaatgc aatcaatacg cttgaagctt cgtttaacgt
    35941 accaggatgt cgatgaaaaa aataggcacc gaaataaaca gaaaacagta agccgcaaag
    36001 agaagacaaa atcaaaaaaa acggattttc ttcggcttct gtgatatcag aatgatccaa
    36061 aagcaaccag cctaatgcac tagaaggcgc atgtgaggtc ttaaggtatg aataacatag
    36121 aaaaaataag atcgtcaaag ttgcaaaaaa agagcctaaa tgtaaaaaat tagtggttct
    36181 acgttgaatt tccataatat ctccaaat
//
LOCUS       DACCXO010000018        34578 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.018, whole genome shotgun sequence.
ACCESSION   DACCXO010000018 DACCXO010000000
VERSION     DACCXO010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.018"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            212..604
                     /locus_tag="IVY70_000831"
     CDS             212..604
                     /locus_tag="IVY70_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7957982.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="IVY70_000832"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="IVY70_000832"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP7957983.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="IVY70_000833"
     CDS             3165..4775
                     /locus_tag="IVY70_000833"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP7957984.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="IVY70_000834"
     CDS             5067..5936
                     /locus_tag="IVY70_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP7957985.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="IVY70_000835"
     CDS             6101..7372
                     /locus_tag="IVY70_000835"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP7957986.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /locus_tag="IVY70_000836"
     CDS             7369..8664
                     /locus_tag="IVY70_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP7957987.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="IVY70_000837"
     CDS             8763..9026
                     /locus_tag="IVY70_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP7957988.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="IVY70_000838"
     CDS             9471..9920
                     /locus_tag="IVY70_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7957989.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="IVY70_000839"
     CDS             9977..11395
                     /locus_tag="IVY70_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7957990.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="IVY70_000840"
     CDS             11395..12255
                     /locus_tag="IVY70_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP7957991.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="IVY70_000841"
     CDS             12429..13202
                     /locus_tag="IVY70_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7957992.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="IVY70_000842"
     CDS             13349..13714
                     /locus_tag="IVY70_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP7957993.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="IVY70_000843"
     CDS             complement(13862..15070)
                     /locus_tag="IVY70_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7957994.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="IVY70_000844"
     CDS             complement(15089..15367)
                     /locus_tag="IVY70_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7957995.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="IVY70_000845"
     CDS             15560..16216
                     /locus_tag="IVY70_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP7957996.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="IVY70_000846"
     CDS             complement(16658..17455)
                     /locus_tag="IVY70_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP7957997.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="IVY70_000847"
     CDS             complement(17452..18636)
                     /locus_tag="IVY70_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP7957998.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="IVY70_000848"
     CDS             complement(18661..19908)
                     /locus_tag="IVY70_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP7957999.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="IVY70_000849"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="IVY70_000849"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP7958000.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="IVY70_000850"
     CDS             21888..22202
                     /locus_tag="IVY70_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP7958001.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="IVY70_000851"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="IVY70_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP7958002.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="IVY70_000852"
     CDS             22887..23531
                     /locus_tag="IVY70_000852"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP7958003.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="IVY70_000853"
     CDS             23546..23875
                     /locus_tag="IVY70_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958004.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="IVY70_000854"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="IVY70_000854"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP7958005.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="IVY70_000855"
     CDS             24863..25687
                     /locus_tag="IVY70_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP7958006.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="IVY70_000856"
     CDS             25680..26027
                     /locus_tag="IVY70_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP7958007.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="IVY70_000857"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="IVY70_000857"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP7958008.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="IVY70_000858"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="IVY70_000858"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP7958009.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IVY70_000859"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IVY70_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP7958010.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IVY70_000860"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IVY70_000860"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP7958011.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="IVY70_000861"
     CDS             31684..32451
                     /locus_tag="IVY70_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7958012.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="IVY70_000862"
     CDS             32441..34483
                     /locus_tag="IVY70_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7958013.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11116 a         6130 c         7259 g        10073 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctctt
//
LOCUS       DACCXO010000019        33547 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.020, whole genome shotgun sequence.
ACCESSION   DACCXO010000019 DACCXO010000000
VERSION     DACCXO010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.020"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            60..1958
                     /locus_tag="IVY70_000863"
     CDS             60..1958
                     /locus_tag="IVY70_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP7958014.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(2257..2976)
                     /locus_tag="IVY70_000864"
     CDS             complement(2257..2976)
                     /locus_tag="IVY70_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP7958015.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            3313..3645
                     /locus_tag="IVY70_000865"
     CDS             3313..3645
                     /locus_tag="IVY70_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958016.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            3632..5635
                     /locus_tag="IVY70_000866"
     CDS             3632..5635
                     /locus_tag="IVY70_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP7958017.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            5654..6124
                     /locus_tag="IVY70_000867"
     CDS             5654..6124
                     /locus_tag="IVY70_000867"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP7958018.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            6145..6729
                     /locus_tag="IVY70_000868"
     CDS             6145..6729
                     /locus_tag="IVY70_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP7958019.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            6744..7730
                     /locus_tag="IVY70_000869"
     CDS             6744..7730
                     /locus_tag="IVY70_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP7958020.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            7720..8031
                     /locus_tag="IVY70_000870"
     CDS             7720..8031
                     /locus_tag="IVY70_000870"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP7958021.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            8053..9834
                     /locus_tag="IVY70_000871"
     CDS             8053..9834
                     /locus_tag="IVY70_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP7958022.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            9827..11203
                     /locus_tag="IVY70_000872"
     CDS             9827..11203
                     /locus_tag="IVY70_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP7958023.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            11210..11845
                     /locus_tag="IVY70_000873"
     CDS             11210..11845
                     /locus_tag="IVY70_000873"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP7958024.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            11875..13230
                     /locus_tag="IVY70_000874"
     CDS             11875..13230
                     /locus_tag="IVY70_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP7958025.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(13422..14816)
                     /locus_tag="IVY70_000875"
     CDS             complement(13422..14816)
                     /locus_tag="IVY70_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP7958026.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            14908..15879
                     /locus_tag="IVY70_000876"
     CDS             14908..15879
                     /locus_tag="IVY70_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP7958027.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            15876..16658
                     /locus_tag="IVY70_000877"
     CDS             15876..16658
                     /locus_tag="IVY70_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP7958028.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16827..17198)
                     /locus_tag="IVY70_000878"
     CDS             complement(16827..17198)
                     /locus_tag="IVY70_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP7958029.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            17332..17775
                     /locus_tag="IVY70_000879"
     CDS             17332..17775
                     /locus_tag="IVY70_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP7958030.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(17818..18627)
                     /gene="yidA"
                     /locus_tag="IVY70_000880"
     CDS             complement(17818..18627)
                     /gene="yidA"
                     /locus_tag="IVY70_000880"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP7958031.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(18646..20013)
                     /locus_tag="IVY70_000881"
     CDS             complement(18646..20013)
                     /locus_tag="IVY70_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7958032.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(19997..20827)
                     /locus_tag="IVY70_000882"
     CDS             complement(19997..20827)
                     /locus_tag="IVY70_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP7958033.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            20990..21427
                     /locus_tag="IVY70_000883"
     CDS             20990..21427
                     /locus_tag="IVY70_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP7958034.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            21503..22123
                     /locus_tag="IVY70_000884"
     CDS             21503..22123
                     /locus_tag="IVY70_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP7958035.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(22227..23186)
                     /locus_tag="IVY70_000885"
     CDS             complement(22227..23186)
                     /locus_tag="IVY70_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7958036.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            23377..23883
                     /locus_tag="IVY70_000886"
     CDS             23377..23883
                     /locus_tag="IVY70_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP7958037.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            23900..24799
                     /locus_tag="IVY70_000887"
     CDS             23900..24799
                     /locus_tag="IVY70_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP7958038.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            24886..25770
                     /locus_tag="IVY70_000888"
     CDS             24886..25770
                     /locus_tag="IVY70_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP7958039.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(25962..26033)
                     /locus_tag="IVY70_000889"
     tRNA            complement(25962..26033)
                     /locus_tag="IVY70_000889"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25998..26000),aa:Arg,seq:ccg)
     gene            26093..27427
                     /gene="rpoN"
                     /locus_tag="IVY70_000890"
     CDS             26093..27427
                     /gene="rpoN"
                     /locus_tag="IVY70_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP7958040.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            27630..28667
                     /locus_tag="IVY70_000891"
     CDS             27630..28667
                     /locus_tag="IVY70_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP7958041.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            28708..29709
                     /gene="gap"
                     /locus_tag="IVY70_000892"
     CDS             28708..29709
                     /gene="gap"
                     /locus_tag="IVY70_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP7958042.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            29819..31009
                     /locus_tag="IVY70_000893"
     CDS             29819..31009
                     /locus_tag="IVY70_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP7958043.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            31113..31868
                     /locus_tag="IVY70_000894"
     CDS             31113..31868
                     /locus_tag="IVY70_000894"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP7958044.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            31984..33282
                     /gene="eno"
                     /locus_tag="IVY70_000895"
     CDS             31984..33282
                     /gene="eno"
                     /locus_tag="IVY70_000895"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP7958045.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        11045 a         5943 c         7098 g         9461 t
ORIGIN      
        1 tgaaaataga gcataacaac tttataatgt tgagtatacc aatttttgga ggcgttttaa
       61 tggaccgcta tttagattta ctttcagaga agttccctac aacggaagat gtcatcacgg
      121 aaatcatcaa tctcgaagca attttgcagc ttccaaaagg aacagagtta tttttgagcg
      181 atatacatgg cgaatttcct gcttttgatc atattctgcg tatcggttca ggaaatctta
      241 aagaaaagat caaagacctt ttctctgaac aaatgacgga aacggagatc agccaattca
      301 ctatttttac ggcttatcca gaatatgtgt tgacgactga ttggtacaaa aaacaagata
      361 aatcccagct gatccatcgt ttgatcgatc ttttacggtt tacgacagtc aaatacactc
      421 gatcaaaagt tcgaaaagga ttgccgaaag aatatagcta tatcattgag gaactcttgt
      481 atatcgatga tcgtatcaac gggaaaaaag actatgtaaa aaaaatcatt gaacaacttg
      541 tcttgatgaa ggaagaagaa aaatttttaa caaaacttgc gcagacgatc caaaaatctg
      601 tcatcgatca tttgcatatc gtgggtgata tttttgatcg aggaactcaa gcagccaaag
      661 taatggatcg aatcatggat ttttcctctg tagatatcca gtgggggaat catgatattt
      721 tgtggattgg tgcgtattgc ggctctgagg catgtttatt gacgctttta cggattgccg
      781 cacgctacaa ctatttattt gaattagaga aagaatatgg attaaattta cgtccattat
      841 tttcttttgc ccatcaaacc tatcagaaga atccagtatt tacccctaaa aacaggaaag
      901 atctatctga aagagagcaa gaagaattag aaaagatcca tcaagcactt tctatcctcc
      961 aattcaaatc agaacatcag ttgttgaatc gacgacctga atttaaaatg gacgatcgta
     1021 aattgttgga aaaaatcaac tatgaggaat caacgatcgt tttagaaggt cagctctatg
     1081 agttgaaagg gacttgtttt caaacgatcc agccagatga tcctaaaaaa ttcttggaag
     1141 aggaacaaaa agtgatcgat agtttgatgt attcattcca gcactcgtta agaatgcaaa
     1201 aacacatgac gtttttgatc gaaaaagggg gaatgtatct cacgaacaat cagcatgtac
     1261 tgttccatgg ctgtatacct gtagatgaaa aggggcagct cttagcattt gaattagacc
     1321 aatcttatcg cggaaaagaa ctgctgggat tttttgaaaa acagataact gaaagcgcaa
     1381 aagatttgac tgcaaatgaa gatttggcga ccgatttgat ttggtatgcg tggagcgggc
     1441 cattttcgcc tttattcggc aagagtaaaa tggcaacttt tgagcgctat tttctcacag
     1501 agcctcatac gcacgtcgaa aaaagcaatc cctattatca cttgcgagat gaagagtggt
     1561 tcagtgaaaa aattttggca gaattcgatg caaaagaaga aggttcagca atcatcaatg
     1621 gacacacgcc agtaaaagta aaaaaaggag agtcaccgat caaagcaaac ggaaaagcat
     1681 ttgttattga cggaggtcta tccaaagctt atcaaaaaac aaccgggatt gctggctact
     1741 cattactatg caattcttac ggtttccaaa tcgtgacaca ttatccgttc ttgcctattg
     1801 aacaacaatt tgcaaaaaaa tctgatcaaa cgaatgtaaa aaaagtgatc gagcagcagc
     1861 tcccacgcaa acttaatcgg aatacgacaa aaggaatgga attacagcaa cagattactc
     1921 aactccaaag gctgttggat tatcgaaagg acgattgaga aaaatgctag ccaacattgc
     1981 tcaatagact aaaaagcgac tgcaacaaaa ggcgtgtcgc agtctccttc ggaattagct
     2041 caaaaaacgg taaaatatta gaagctattt ttctgtttat ttctctaata ctcaggtcag
     2101 ggcgctgatt tcacgacctc ttccttaaac ggtgttctcc tgccaactcc tcggtcacaa
     2161 gtcacaatat ccaacaaaca tgagaagctg ttttttgaaa ttgctccttg tgactcagag
     2221 ttgaacggca ggctcacgac ctaatcagaa aaacgattaa aattcattca caaagaaatc
     2281 gaataaacgc tgttcagatg aatctacacg atgagaaagt tccggatgcc attggacacc
     2341 aaggattttg tgcatattat ctttactttg gactgcttca atgagtccat cgttggaaaa
     2401 ggcgatggct tttaacaaag gagaaagatc tttgactgcc tgatgatgat aggaattcac
     2461 aaagtatgaa tcagggagaa ggtcacttaa taaactgtcc gaaacgattt tgacttcgtg
     2521 agttgcaaac tgcggggcag tgggctgttg ttcgtgtttt acggaccatt ttggatataa
     2581 tgaaagatct tgataaagtg ttccctccaa cacgacattc aatagttgaa gtcctcggca
     2641 gactgcaaaa atcggttttc tttgtttcaa tgcttccaag atcaatgcct gttcgaattg
     2701 gtctcgttga atatttgttt ccgtcagttt tggatgaggg tcttccatgt aaagttgcgg
     2761 actaacatcc tgaccgcctg caagaatgag tttgtcgatt ttctcaatta gttgaggcgc
     2821 tgaatctgga gacatgactg gcagaataag aggtaatcct tgagcatcta aaactgcatc
     2881 cacaaatccc tgcggtgtat agcttacttg atttccttgg aaagtatctg tcgctcgaat
     2941 cagttgattc ccggcaatgc cgatgattgg tttcattttt attcccccta aagtagacta
     3001 attgtaacaa ggatttaaaa aaatttaaac tatgtaagac ttgcccctat acttatctgt
     3061 tcttgctgtg ttactatctg taaagagaat tttcgtgtga aattcatgac gatagttgaa
     3121 aatattgctt aatttgttat aataaaaagt aagcagaaat aaaaaggttg ggcgtaagct
     3181 tcaaggatct agttcttcaa ggggtgagaa ggacatttta ttgaaggggc ctgcctatac
     3241 taagttatct ttagaggtac atactttcaa tgaaaaaaga aagcatgtac ctctttttta
     3301 aatcgaaagg aagtgaagca agtgttagaa gctttggaac aaatccgaaa agcagaagaa
     3361 gaaaatgaga agcgtgctcg aaccctacgg cagcagttga agacctatgc agaagaaaaa
     3421 gagcaagaat tggagataat ccaaacgaac agtcggcaac agctgaaaca gttggtagaa
     3481 gaaaaagaac aattcgaaaa gcaagaatta gaaaaagaaa aaatgaaatt gaaagcagaa
     3541 gcagacaagg tgcaagcaac tttgaaagac caatatcaaa agcatcagag tcaagcaatc
     3601 ggtgcaatca ttgaaagggt gaaagaaact tatggccgtc actaagatgg aaaaagtgac
     3661 cctcatttca gataaaagaa atcaggagag gattttacaa gcagtacaag gaatccaaaa
     3721 tgttgaaatc cgggatctgt atcaaaatag aacaaacaac cagtgggtag aaaaatattt
     3781 tccagatacc acgattattg ataaagaagc aaaattaagc gctttaggca atcggctgaa
     3841 agacatccaa gaagctatcc aattcataca acaccacggc gatagccgtc aaaagaaact
     3901 ccatttgaaa cgtcaagaac tttccttaca agacttggaa gcaaattatt ccgaagaaaa
     3961 tttcatgaat aaattagaag aagtccttga tttaaaagaa aagtgggaac agctaactga
     4021 aaaacgtgat cgtttagaag aagaggaaga atggcttctt cattggcaac agctggatat
     4081 tgctccaagt atgtatgcaa gtaagacgac catttttgag atggggactg tgagtgcagc
     4141 aaattttgaa agtttcaagg aagaactaag taagcttgat gacgtttata tggaagaaat
     4201 agattatacg ccaaaacaag tttatctttc gtatgttgct ttaaaaaaat cagttgctgc
     4261 tatagaagaa attgctggga aatacgggtt caataaagaa acgtatcctt atgaacaaaa
     4321 tcctaaagaa aaattgcaag aaaccaaaca gcaattatcc attgttcacg agcagcaaaa
     4381 aaagataacg acagcgttag gtcagtgcag cggctatatc caagaatttg aatgggtaga
     4441 agaagtcaca cttgccgttg cagaacggga aaaagtaaaa gaacggttta tccatgccac
     4501 ttatctgatc gttttgcaag gttgggtaga tgcagaagaa aaacagaatt tgatgcatgt
     4561 gttaaacgaa actgtatcag aagaagatat atacgttagt ttcgaaacac caaactcaga
     4621 agagattcag caggaagtcc ctactaaatt gaaaaaccat ccgcttatcg aaccttttga
     4681 attgctgact gaaatgtaca gtctgccaaa atatgaagaa gtagatccaa cgccttggat
     4741 gacaccattt tatcttgtct tcttcgggat gatggtcgca gatgttggtt acggattgct
     4801 gatgctcatt gggacaatac tagctcaaaa actatttgtt ttaccccgcg ggatgaaacg
     4861 tttcgtaaaa ttctttgaga ttctctcggt accatcgatc atctggggat tgatctacag
     4921 ttcatttttc ggacaggcat taccaaaaga actatttggt atccgcttgc ctttcccaat
     4981 cctatctacg acggatgacg tcaatacgat tttgatttta tccgtgattt tcggtctgat
     5041 acagatcttg gttggattat tcgtggcagc aaaggaaaat atcaggagga aagattactt
     5101 gagtgctgtc agtgatggtt ttgcctggca agggatcctg attgggatcg ttattgcatt
     5161 gattggtgtc atgttgataa aaaatacagc atttgtttat cttggcgcgg ggctatcgat
     5221 cattgctgct ttatgtatct taatcgtgcc gattatccaa tcgccgtcta aagtcaaggg
     5281 tgctgccaaa ggagcgtata atctttacgg attgactggt tatatcggtg atttagtcag
     5341 ttatacacgt ttgatggcac taggtatttc aggcggaagt attggcgcgg cattcaatat
     5401 gctagttgct ttcatgccgc cagccgctcg attcagtgta gggatccttt tgatcatcgc
     5461 tttacacgca ctgaatatgt ttttgacact gttaagtgcc tatgtccatg gtgcacgatt
     5521 gcagtatgtc gaattttttg gaaaatttta tacaggaggc ggacgtgcct ttgacccgct
     5581 gaaaacggcg gaaaaatacg tcaacatcaa tcataagaaa aaaaacaata aataattgga
     5641 ggatttttat acaatgatgg attacttaat tagtcaaaat ggtggaatgg tatttgcagt
     5701 attagcgatg gcaacagcga cgattttttc agggattggt tcagctaaag gtgttggaat
     5761 gacaggtgag gcagcagcag cgttaacaac aagtcaaccc gaaaaattcg gacaggcttt
     5821 gattctgcaa ttgcttccag gtacgcaagg gttgtatgga ttcgttatcg cctttttgat
     5881 ttttatcaac ttaggaaatg atatgtcagt tgtacaagga ttgaactttc taggcgcttc
     5941 tctgccgatt gcttttacag gtttgttttc aggtatcgcc caagggaaag ttgcagcagc
     6001 aggtatccaa attttagcga aaaaacctga acatgctaca aaaggaatca tttttgctgc
     6061 gatggttgaa acatatgcga ttttaggttt cgttatttcc ttcctattag tattgaatgc
     6121 ctaagcataa aggaggaaaa gcagatgaac gccattgaaa aaatcatctc tcagatgaat
     6181 gaagcagcag agcaagaaag agcggcgtta gagcaagaag agcgaatgaa gatcgatcaa
     6241 aactttgaac aaaaacgtac gcaaatcgaa acagaacatc aaaaacaaaa agaaaaacaa
     6301 atcgaattac ttgaaaaaaa ataccgtcag cttaggaatc gccaacaagt agaagtacga
     6361 caagaaaatt tgaatgcgaa gcaggaattt ttacgacgat tgtttgcaga tgcaatagct
     6421 gaaatggaga attgggatga atctgaacaa atccagttta tcaaaaattc attgtattca
     6481 ttacctttga caggcaaagt aacattcatt gctggagaaa aatcagcagc ttatctttct
     6541 cagacattgc tggatgaatg gaacaatgaa cttcccttta tgatggtttt aagtgatgaa
     6601 actgttgctg atcaagcggg ttttctgata aacgatcaag gagttcagta taattttctt
     6661 ttcagcagcc ttgtacaaga tatccaagga acgatgagtt ttgagattgc caatcaatta
     6721 ttcgaataag gagggatcag tgtatggaat accacgaact aaatcccttg attcgcggaa
     6781 gagagcttga acttttgtcc aatgagacat ttgaacggat gattcaaaca aattctgtcg
     6841 aagcacttgg tgaaatattg aaatccacga tttacagtcc atatatttat gacggatatg
     6901 aatatgactt cgaacagaat cttttaaagg agcagagccg tctatttcgc tggttaaagg
     6961 aatcagcacc ggagcctgaa atcgtttgga tctatacgat gcgctatact ttccataatc
     7021 tgaaggtttt gacaaaagcc gagatgacaa atcaaaatct ggaccatttg tatatcgacg
     7081 atggatttta ttcattagaa accttgaagg atgccattca tacgcaagtt tcaaatgaac
     7141 tccctgaaag cattatggac agtatacgag aagtacgtga ttattttgaa gagtctacca
     7201 ttcttcaggg aattgacgtg atttatgatc gacagttttt gacagagcag cgacggcttg
     7261 ccgataagct aggatatcaa gaattgctga cagaagtcat tgcttttatt gacttgacca
     7321 atatcacaac gatggctcga ggaatattgc agaaaagaac atatgggttc atgacgacgg
     7381 tactttccag ctcgggaagt atcgaaaaag aagtattttt gaactttgta gataaagata
     7441 tggaagatta cacgaatttt ctattagaga cagattatgc tgattttctg cgccctgtca
     7501 tccgtgaggg aaatcttgat ctcgtaaagc ttgaacagtt gaaagatgat tatctaagtt
     7561 cattgtatca agaagcacaa acccaggctt tcggaccact gccactacta gcctttttga
     7621 acgcaaaaga ggtagaaagc aaaaaccttc gtttactggt tatcggcaaa agaagccatt
     7681 tcgaaccaga aacaatcaga aagcgggtga gacaagtcta tgacgcataa gatcggtgta
     7741 gttggcgaca aagattctgt tttgccattc aaattgtttg gctttgatgt tcgttatggt
     7801 acaaccaagc aaaccgttcg tcaggcaatt gaagaaatgg caaagaatga gtatggcgtg
     7861 atttatgtca cagagcaatg tgcagcattg agcccagaaa cgatcgaacg ttacaaggaa
     7921 caaatgatac ctgctatcgt attgattccc aatcatcaag gatcattagg gatagggctg
     7981 gaagaaattc aaaaaaacgt agaaaaagca gtaggtcaaa atatcttata gtatgaaaag
     8041 gagatagaaa ttttgcaaat tggaaaaatc ataaaagtct ccggacctct tgtaatggct
     8101 gagaacatgt ctgatgccag tatccaagac atgtgtctag tgggagatct aggagttatc
     8161 ggagaaatta tcgagatgcg tggcgatgtg gcgtctattc aagtatatga agagacttct
     8221 ggaattggtc caggcgaacc ggttcgctct actggtgaag ctttatctgt tgaattagga
     8281 ccagggatca tttcacaaat gtttgacgga atccaacggc cattagatac ctttatggaa
     8341 atcacacaaa gtaatttctt agggcgcgga gttcaattgc ccgcattgga ccatgagaaa
     8401 aaatggtggt tcgaaccaac agtagaagcc ggagaaacgg tgagtgctgg agatgttatc
     8461 gggatcgtag aagaaacaaa agttataaaa cataaaatca tggtgccaaa tgggataaaa
     8521 ggaacgatca aaacaatcga aaaaggttca ttcactattg atgaaacagt atgtgtcatt
     8581 gaaaccgaaa atggtgacaa aaatttgacg atgcttcaaa aatggccagt aagacgtagt
     8641 cggccaatca aagaaaaatt aaacccggat gcacccatga tcactggaca gcgagtgatt
     8701 gatacattct tcccagtgac aaaaggagga gcagcagctg tacctggtcc ttttggtgca
     8761 gggaaaacag tcgttcagca tcaaattgca aaatgggccg atgtggacat ggttgtctat
     8821 gtgggatgtg gcgagcgtgg aaatgagatg acggatgttt taaatgaatt ccctgaattg
     8881 attgacccta atacaggaga atcgttgatg gaacgtacgg tattgattgc taatacatca
     8941 aacatgccag tagcagctcg agaagcttct atctatacgg gaatcacaat tgcggaatat
     9001 ttccgtgaca tgggttatga cgttgctatc atggcagact ccacttcacg atgggcagaa
     9061 gcacttcgtg agatgagcgg acgtttggaa gaaatgcctg gtgatgaagg ttatccagct
     9121 tatcttggtt cacggcttgc ggaatactat gaacgatctg gaagagtcat tgcattagga
     9181 tcagaaggtc gcgaaggaag tataaccgca atcagtgctg tatccccgtc tggaggggat
     9241 atttcggaac cagtcacaca aaatacattg cgggtcgtaa aagtattctg gggtcttgat
     9301 tccagtttag cccaaaaaag acattttcct tcgatcgatt ggatacaaag ctattctctt
     9361 tattcaacag aagtagggaa gtatatggat catatccttc aagctgactg ggcatccatg
     9421 gtcaccgagg gcatgcgtat tttgcaggaa gaacagcagc tgaatgaaat cgtgcgtttg
     9481 gtagggattg attccctttc agataatgac cgtctgacat tagaagtggc aaaatccatt
     9541 cgtgaagatt atttgcagca aaatgctttt gatgatgtcg atacattcac ttcaagagag
     9601 aaacaattca aaatgttgaa gatgatcttg acctttggtg aagaaggacg ccgtgcgttg
     9661 tcgttaggtg cttatttaag tgagatcatg gacggtaccg tatcgattcg tgaaaggatc
     9721 ggacgaagca aatatatccc ggaaacagaa ttagaaaaac tagatcaaat tattggtgaa
     9781 atcaagcaaa cgatgcaaac gatcgtttca gaaggaggca tgagcaatga ttaaagaata
     9841 tcgtacgatc ggtgaagtcg tcggtccatt gatggcggtt gaaaaagttg ctggtgtcaa
     9901 atatgaagaa ttaatcgaag tccgtatgca aaatggcgag attcgtcgtg gacaagtatt
     9961 ggaagtccaa gaagataaag caatggtcca aatattcgaa ggaacaagtg gtatcaattt
    10021 acgtgactct tctgtacgat ttttaggcca tcctttagaa ctaggtgtat cggaagacat
    10081 gatcggtcga gtattcgatg gtttgggacg accgaaagat aacggaccag agatcctgcc
    10141 tgaaaagcat ttggatatta acggagaagt aatcaatcca gtagctcggg attatccaga
    10201 tgagtttatc caaacaggta tctctgcaat cgatcatttg aatacattag ttcgtgggca
    10261 aaaattacca gtattttctg gctctggtct tcctcataaa gaattagctg ctcagatcgc
    10321 gcgacaagca acggtactcg attcatcaga tgatttcgcc gtagtctttg ctgctattgg
    10381 aatcactttt gaagaagctg aatttttcat ggaagatttt cgtcaaacgg gcgcaatcga
    10441 ccgttctgtc atgtttatga atttagcaaa cgaccctgca atcgaacgta tcgctactcc
    10501 tcgtatggct ttgactgctg cagaatatct tgcttatgaa aagggaatgc atgttttggt
    10561 tatcatgaca gacatgacca actatgcaga ggcattgcga gagatttctg cagcacggcg
    10621 agaagttcca ggaagaagag gatatcctgg gtatctttac acgaacttag ctactttatt
    10681 cgaacgagca ggaagaatcc gcggactaaa aggatcggtt actcaaattc cgattctaac
    10741 aatgccagaa gacgataaaa ctcatccaat ccctgacttg actggttata ttacagaagg
    10801 acagatcatc ttgactagag agctttacaa gagtgggatt cagccaccaa ttgatgtact
    10861 cccgtctctt tcgcggttaa aagataaggg aactggaccc ggaaagacaa gagaagacca
    10921 tgcagctacg atgaatcagc tttttgcagc ctatgcacaa ggaaaacagg caaaagaatt
    10981 agccgtagtt ttaggtgaat ctgctttatc tgatgtagac aagatctatg cgaagtttgc
    11041 tgagcggttt gaaaaagaat atgtcaatca aggattctac acgaaccgtt cgattactga
    11101 aacacttgat ctagggtggg aactgctatc gatgttgcca agaaccgaat taaaacgaat
    11161 caaagacgac atgctggaca aatatctgcc agaagggaag tgagttctga tggcacgatt
    11221 gaatgtgaat ccgacccgca tggaattgac tcgattgaaa aaacagttga caactgccac
    11281 aagagggcat aaattattaa aagataagca agatgaactg atgcgtcaat tcatcctgtt
    11341 gattcgaaag aacaaccagc tgcgtcaaga aatggaagag tctatgacag aagcaatgtc
    11401 tgattttgta ttggcaaatg cttcgatcac ggaacctttc atcgaagaat tatttgtttt
    11461 gccggcggaa aacgttgatt tgtctatcgt agacaaaaat atcatgagtg taaaagtacc
    11521 tgtgatgaat tttgaatatg atgaacagct gacagacaca cctttggaat acgggtattt
    11581 gaattccagt gccgaactgg atcggtcaat cgatcggttt actaccctgc ttccaaaact
    11641 tttggaactg acagaaatcg aaaaaacttg tcaactgatg gcgagtgaaa tcgaaaagac
    11701 acgtcggcga gtcaatgcat tagaatatat gacgatccct caattagaag aaacgatcta
    11761 ttatattcgt atgaagctgg aagaaaacga gcgcgcagaa gtaacaaggt tgatcaaagt
    11821 aaaaaatatg ggtaccactg actaagtggt atcttttttt aaaagaggaa aacgatgatt
    11881 aaacaaagca aacaaataaa gaaacgtttg ccggctgttc agcttctcgc attaggattt
    11941 tttctcctga ttttagttgg cggcggccta cttaccctac cattttttag taatagtggg
    12001 catgggacaa attttatcga tgcgcttttc actgcaactt ctgctgtctg tgtaacggga
    12061 ttgacaacat tgaatacagc agagcattgg aacagtgctg gtcaattttt gatcatgctt
    12121 ttgattgaga tcggcggtct tggttttatg atggttccga ttctatgctt tgcattgata
    12181 aagaagaaag tcagtctcag cacacgtatt atattaaaag aagcgctgaa tctagaagaa
    12241 gtatcaggtg tgatgaagct catgctttac atcctaaaat tggcctttgt tatccaattg
    12301 attggtacgg ttgctttatc ttttgtattt gtgcctgaat ttggctggtt gaaaggtatc
    12361 tggtacagtt tgttccatgc aatctctagt ttttgtaatg cagggtttga tttgttagga
    12421 gacagtttag ccaatcacca gcaaaatgtt tatttgatca tggtggtatc tgctttgatc
    12481 atttctggtg ggttaggctt catcgtatgg cgtgatttac tggaatatca tcagaaaaag
    12541 aagatgacga ttcattcaaa aatcgcatta accgttacag gtaccctttt attaggcgga
    12601 ttcgttgtgt tctttttcac agagcgcaat ggggcaacgc tagtggaagg gaatatgttt
    12661 caacgattgg caaacacctt ttttatgagt gtgacaccta gaacggctgg atattattca
    12721 gttgattacc tccatatgag ccatgcaggt ctgattttaa cgatgttttt gatgtacatc
    12781 ggcggaacat ctgggtcgac ggccggcgga ttgaaaacaa ctacgttagg tgtgttgctg
    12841 atccagatgc gttcgatgtt gaaaggacgt acgagagcgg aagcatttgg ccgtaccatc
    12901 cgacaaggcg cagtgttgcg ggcacttact ttattttttg ttacgttgac gttgtgtgta
    12961 gtcgccatca tgattttatc ggtgactgaa acgataccta aaacttctgg catcgaatat
    13021 attgcttttg aggtcttttc tgcgtttggt acagtaggtc ttacgatggg attgacgcca
    13081 gacttgactg caatagggaa attgatcatt atagcgctta tgtacattgg acgagttggt
    13141 gtattgactg tggtcttctc tctgatgaca aaagcacaaa gaacacaatc aaaatacaaa
    13201 tatccagaag aaagtgtcat gattggataa tttcatcttc tgacagaaac ctagtgattt
    13261 agtaaaataa gaaaataaaa taatttttct tgcttcttgg atactttaag tgcaaaatgt
    13321 tcgtttattt tttgaggcac tacccgttgt tgaacctcgg gtagtgcctc ttttttattg
    13381 aaaggaatca ataagatttg attcctttat ttatcaatag gttataattc atattttttt
    13441 attttttctt ttacgatact tagtccagaa gctaaaattt caaggttatt agaggatagg
    13501 gaaattcgaa cgaatgcatc tggttgcgca cgtgtagtaa agcgtctgga atgataaagt
    13561 cggataccgt tagcaagaaa gtcattctca attatcttag ggtcaatctc aggatcaact
    13621 ggaagaattc gatagtaagg gacacgattc gttggttttt ctactttgaa aagagaatca
    13681 aaaagctgat tggctctttt tgtaagactt aatttagttt ctaagatcct atttgcttca
    13741 tttgaatgga ggatttgagt gatgacttcc acatctaatc cagaagtttt cacatttata
    13801 ttaaaaattg cttgttctat cttgctttgg acatgtactg gaactacaag ataagcaact
    13861 cgcaatccag aacaaataaa ttttgtcatt ccagctaagt agattgtttg gttaggtaat
    13921 aaattttgga aaggaggaat gatcgtatgt tgatagtaag ttgttaaaaa tgaatggata
    13981 tcatcttcaa taacccatag accttctttt tcaatcactt cggctaattg ctctcgtctt
    14041 ttgtgagaga tttgaaaccc tataggatta ttacaagaag gcataagaaa gacaccatga
    14101 atcttctttt ttctacattc ctgaatcaat atatcaggtc gcattccttg ctcgtcaaaa
    14161 tcaattggaa cgatctctaa atgatataac tgagccaatt caatgaaatt agcgtatgta
    14221 tatcggtctg ttgctattct atcacctgga gagaaaagtg cagaaagtgt gatagccaat
    14281 ccattttgaa caccggaaac aatagataga gattctaagg acggaatgac tccttgagtt
    14341 tttaaccaat ttgctgcagt agtcaattga aaatttgtgc ctttgggatc ttggtaatta
    14401 agcaaaccaa caagatttgg gttattacta gttgattgaa taaacgggac aacgaattta
    14461 ttacattctt caaaagaact aaccagacca aaatcaatga tatgttcggt tacttcattg
    14521 gtagagatgg tgatggactc aaagacattg ggagaaataa atgtgccact tccaatattc
    14581 gtatacagca aacctttctc aattccatat ttataagcct tgccaacggt agtaaaattt
    14641 aaatctaaat aatcagctag ctctctttgg gagggtaact gagtgttttt ctgtaattta
    14701 ttctttaaaa tatcttgttc taattgttga atcaacgatc gataataagg acgcttcaat
    14761 ttttgtttgt ctggtcgcca agttaacggg taattgtcat aagaattaat aggcattttc
    14821 tccactcctt aaaattgtat accatacaat tttaccattg attgtattga ttttagaagt
    14881 taatcttttg tgaggaggga attgtctatg aaaattatta attttgagga agtagtagct
    14941 tcttttagtt ttgaagaagc gattcaagta atgaaaaaat gtttttttga ttatgaaagt
    15001 ggtaaaatca gccaaaatcc tcgattggtg gaaattttac ctgacggaaa aggcaaaaat
    15061 atgtttgcga tgatgcctgc ttatttgggg gagaacagat attttggcac aaaagtcatt
    15121 accgcttttc cggataatca tcagtatcat ttaccttctc atttaggaga gattttcttg
    15181 tttgatgctc aaaacggact acccaaagca ttgataaacg caaatgcagt cacctggatt
    15241 cgaacagctg ctgtatctgc gctggcaaca gattacttag caaatccgca tgcagaaaca
    15301 cttgctttaa ttggagctgg tcaacaagca ttgtcgcatt tgactgcgat ttctaccatt
    15361 cgtcccatca ctacagtgtt tgtttacgat attttagaag aaagacgaaa tacttttatc
    15421 gctaaagcta aaaaacagta tcctaaaata acgtttataa atgcaatatc agtagaagat
    15481 gcagtacgtc cagcggaaat catttgtacg ttgacctcta gtaaagaggc atttcttcaa
    15541 aaagagtggg taagggaaga cgcacatatc aatgctgtgg gcacatttac tcctgataca
    15601 agagagataa gtacatcgtt gatggaaact tctcaaatct atgtagatga ttactcagct
    15661 gccttaacag aaagcgggga cctgcttata gccatggcag aaggagattt agaggaatct
    15721 tgtattgttg gtacattagg cgaattagtt tctgagaaaa aaacatttta taaaggagaa
    15781 tcgaggccga cgattttcga cgcagttggt ctagctattg aagatctttg ttgtgcagaa
    15841 tatatttata acaaattaga gaaaggggct ttaaaatgaa cttagattat tacgtagttg
    15901 atgcatttgc agatgaggtg ttcaaaggaa atccagcagc ggtttatgtg ctagaagaat
    15961 ggctgccaga aggtacgatg caaaaaattg ccattgaaaa caatttatct gaaacagcat
    16021 ttacagtgaa gaaaaaccaa gaatttgagc ttagatggtt tacgccagat cgagaaatcg
    16081 atctttgtgg tcacgcgaca ttagcaacag catttgtttt atttaactat tacaaaatac
    16141 ctgatgaaac gattaaattt tctactcaaa gcggtaatct atttgtgaca agaattaaag
    16201 attattatta catggatttt cctagtatta tgccgaaaaa agtgccgata ttgtctgaat
    16261 atgaagaagc gattggggct aagattaaag aagcctactt agcacgtgat ttatttttcg
    16321 tattagaaga tgaaaaaacg gtggctaaat tgcagccaga ttttacagct atcaaaaatt
    16381 ttgaattagg aataggagtc atcgtgacag cagaaagtgt gcaaaaagac tttgtttcta
    16441 gaaccttctt tccaaaatta acaatcaatg aagatccagt atgtggatca gcacattcta
    16501 atcttattcc ttattgggca gaaagaatga ataaaacaaa attgtcagct tatcaaatgt
    16561 catcaagggg cggggatcta gaatgtgaat taaaagaaga tcgtgtgata ataggaggaa
    16621 aagcagtttt gttttctaaa ggtacctcgt ttatttgatt tctttcaaat gaattaaaaa
    16681 taaattggtg gagagtggca accttatagc tacaatggtt gttaaaaaaa gacgcaaact
    16741 ttttcaattt aatgaaaagt ttgcgtctca ttttaattat tggtgtttgt tttttattca
    16801 atcaagagta aaatgcccca tactaactat cctcggatga cttcgatttt ataaccatca
    16861 gggtcaatga caaagtagta agaaggcgca gtgccaggta atccttttaa gtcagtaatc
    16921 tcaagaccgg cagctttatg ttgctcgtgt aatttttcta gatcatcagc tgctatagcg
    16981 atatgaccat agccgtctcc tagatcatac gcaccatgat catagttgta agtcaactct
    17041 aattcatagt catccccagg aagtgtcaaa taaacaagtg taaatttgtt ttctggaaaa
    17101 tcacggcggc ggctttcttc aaaaccaaat gccttttgat aaaattcgat tgatgcttca
    17161 agatctttca ctcgaacaca tgtgtgagcc attttcatag attcatctct ccttttcctt
    17221 ttactcaatc ataacaaaaa gaagtaagga aatagttgat ttgtgcttgt tcatgagaaa
    17281 tttctcatgt aaaatggata gaaaataaaa ctgttaaggt ggaaactatt catggataaa
    17341 ccaatttttg gtgaaaaaaa gcaatttgct gattatcaaa cccgttatgc agcatatatt
    17401 gtcatagaaa gagatcagca gattgcagta atcgaggctc caaatggtgc tttttttctt
    17461 cctggcgggg aaattgaagg agaagagacg aaagaagaag cgattgtccg tgagctgata
    17521 gaagaaatgg gaattgtggc agaaatttct tcttatctgg gagaagctga tgagtatttt
    17581 tactcgaatt atcgtgcaac ttattattat aatccaggat atttctttgt tgctgattct
    17641 tggaagcgta taggagaacc aactgaaaag accaataaaa tttggtgggt gagccctcaa
    17701 gaagcgcttg tgaaattgaa acgtggtagc caccgatggg cagtcgagag atggatcgaa
    17761 aaaaataaaa actgaaagaa agcgagtaga tcagctttct ctcagttttt ttatttatta
    17821 cagtacatat tttttgattg cttcagctac accgtgctcg tcgtttgatg aagtgatgac
    17881 atttgctgct tctttgacaa gagaaacggc atttcccatt gctactccta gaccagcata
    17941 ttcaatcatg gataagtcgt tctcgttatc tccgatagcc atgatttcat cttgatcaat
    18001 accaaggtgc tgtgcgagat tcgcaactgc cgcaccttta cttgcttctt tatttagtac
    18061 ttcgaagtaa tagggcgcac ttttgaccgt tgtgtattta tcacggaaag tttgcggtaa
    18121 acgcgcaatt gctgcatcta aaatttctgg ttcgtcgatc atcatcattt tgacgatgtt
    18181 catgtctggt gtcatttctt caggtgttcg atattttagc ggcatcttga ctaaacttgc
    18241 ttcgtggatc gtataagcgc tgatatctcg attaggcgtg tagatcgctt tgtcatcgat
    18301 cgaatggaga tgactgccga cacggcgtgc catcacttcg atctctagga aatcttcatg
    18361 tgtcagtccg tgacgggcga tgattttacc tgatttcgtt tgttgtacga gtgccccgtt
    18421 ataagtaata acgtaatcat tatcttgaaa aaggttcaac tcgtttaact gttcctggac
    18481 acctggtaag ggacggccag tgcataaaac gatcgaaaca ccttttgcaa cagcagcggc
    18541 aatcgcttct tttacttctt ttgttacttc tcttttctca tttaataaag taccatcgat
    18601 gtcgatagcg actagtttga tagacataat ttcctccaat atattttaat ttatttcaat
    18661 caatgcgcca ttacggatat ggctggcaaa ttcttgataa gtttcgtcaa ataaatcata
    18721 atgatcacgc aaggacggat caaccatttc ttttggaaag aaaaatcgct catctccttg
    18781 ttcttgtccc gctaatgcag caacaagctg actaacctgt gataattcta tcaaagttcc
    18841 gtcatttcgc aaaatctcga tttgtgtgcg atgtctgcct tgttctgggc gataaaaatc
    18901 ataaggtaga tcgtagctag aattcacagc agtgtaataa acaggattat atccgacctt
    18961 ttctactaac aaagtgagtt cttggactaa ttttgaatcc cggttgccag aaaaagtagc
    19021 agatttcagt ggtttacgat tcaaaaaacg tttagctaaa tcatttaaaa taggatcagc
    19081 agattcagac cattgagtaa agtaagtgcc caagacccca tcatcaagtt tgaggtattc
    19141 ttctaaagta aagcttccgt caagaaatgg cagaagtaat tgagaatgga gttcaaaact
    19201 gctggcatct tcttgataca actcgtgtgc gcgatgaagc agatgctcca aaattacttc
    19261 cataccacgg gaaacaggat ggaagtacac ttgcacatac atttgatatc ggctaacgat
    19321 ataatcttcc actgcatgca tgccactcat ggaaaaagcg atcccgcctt cataaggacg
    19381 aatcacacgt aatattcggg taagatcaaa tgttccatat tctgttccag tgaaataagc
    19441 atcccgaagc aaatagtcca ttcgatctgc atcgatctga ctagaaatca tttggacaac
    19501 ttgtgggttg gggtaagttt tttggatcac actagctacc ttttcaggaa aaccttcttc
    19561 tactcggtta agaatctgat acacttctgt ttctggagaa gtgatgatct ctacagtgat
    19621 ggcttcatga ttcgtatgga agatatgttc aaatgtatga gagtaaggac catgaccgac
    19681 atcatgcaac agtgccgcac ataaagtcac cagccgttcc tgatcatccc agcctccgtt
    19741 gcctattttt tcttttgaaa aatttcgaga aaagatatcg cagattcgtc ttgaaatctc
    19801 ataaacgcct aatgaatgag taaaacggct atgttctgct ccgtgaaaag tgaaagaaga
    19861 agtgcctaat tgtttaatgc gtcgtagacg ttggacttct ttcgaattga tcaaatctaa
    19921 gattacttga tgctggacat ggatatagtt atggactgga tcacgaaaaa ctttttccat
    19981 aggcagcatt tgatatttat aaggggttgt cataatgtag ctcctttatg ttattacgtt
    20041 gttccattct ctggatttgg ttgttccatt gctccagctg gatttctttt tttagccagt
    20101 cggtagaatc ctgtaaatca cttgcagaat acagcttttc aaaagctttt gcaaatgctt
    20161 ctttgacttc ttttacaaaa aggggttggt caagcagttc ttcaagtgtt gccattgtag
    20221 caggatccac tgcaggatag ccatttgtac caaagtcttc ttttaaagca gattgataaa
    20281 atcgcttcat gacttgcccg cgtttttcct gattaccgtt gacacttaag tacatcataa
    20341 cagaaatccc atttttgatc cgacgctgag cgataccggc aaattttttg ccatggatgc
    20401 ttaaatcata agttccagga caataagaat gggtgatttc gaatgcttgt atcggtgcct
    20461 ttggaaaagc gtatttagtc aataggagca tttgctcgta agcgtcatct atggttattt
    20521 tttttgtgtc actttggggg aaaatcaaag aaatattcaa aattccttca tctgatacga
    20581 tccctaatcc acctgaattt ctgatcactg gagcgtaacc tgcatctttc agtgtttgga
    20641 tcccgtcact tagaaaagga gttcgaacat ccttcatacc taagatgact gtgcagtcat
    20701 attgccagaa atgtaaaaag atttgctggt gttctttaga atattccgtt aatatatctg
    20761 ttaatgcaaa aggggaaaag tcgttttttt gataaatgtg ctgatctagt cgaatcgcag
    20821 ggttcattca accggctcct ttgtataaat tcatattttc tacaatatta tactctaaaa
    20881 tcaaagagtt gttttggaaa aaaggttttc tgtaaaaatg gacaagttgg ttgacaaaac
    20941 aaaagaaact aggaacaatg tagctatgag aagtaaagga gaatgaataa tggaattatc
    21001 acaaggaaca ccgatttcaa tcaaattgcg tacaaaagta aaacagcaag gggaagtgca
    21061 agatttttat tttgatttga aagggcaaat ggtgacaatt ggcgatacgc tgtacatacg
    21121 ttataaagag attcaggagg agacgggcga tgagattcct gtaacgatca aattagttcc
    21181 agatggtcat gtacagctga tccgtgcagg agaaatgcgg atgcgtttga aatttggcta
    21241 taaagaacgg ttagaaacat cctatcgaac accatacggt atgatccaga ttgctacttt
    21301 tacgaaagaa ctgcatgtta gtttgaaaga ccgtcccaca ggagggaagg ttcgaatcga
    21361 ttatgatctg ttcatgggac cggaaaaaat cggcgaatat tatttgacct tggattttac
    21421 agcgtaaata taaaagaaaa gtttgatttt ttgtgtctga ttttgtatga taaggagtag
    21481 actgtgaaag gacgtgtcct gattggaaat tagtgtattt gaaggtgcaa acaaaagcga
    21541 attatcaatg atcgaagtag cacacgcgat cttagaacaa cgtggcgatg tcatggattt
    21601 ttctgattta gctaaccaga tccaaaacta tcttgggaaa tcagacagtg aaattcgtga
    21661 ttcactcgca caattttata ctgacttgaa tattgacggc agctttatct cattagggga
    21721 taatcgttgg ggcttgcgtt catggtatgc tatcgattcg atcgatgaag aagtaaacca
    21781 tggaatggac gaagaggatg aagatactcc acgtcgccgt aaacgcaaga aagtcaacgc
    21841 ctttatcaat gatgatgaag acgcgatcga ttacaatgac gatgacccag aagatacaga
    21901 tctgacagaa gaagatgacg atgatctgtt tgatgacgat gatgatgaag atgaagaaat
    21961 cgctgcttat aattctgact tgcaagaaat cggagcagat gacccgacag atgatgaaga
    22021 agacttgcca ggaaatatcg aagaagattt aagtatcata gacgaagacg atgatgatga
    22081 agattatgaa gaggaagaat tttctgactc tgaaaaagaa taaacattat tttcttacga
    22141 gaggttgtga cagagacgat cagcctatca gaataaggag gtgtgacacg atttttgtca
    22201 caccttcttt tttgtattct taattgtcaa attaagttag acaaatcatc aatacttacg
    22261 taactcaaaa atacttccaa aggtgttcga taattcagtg attttctagg aatattattt
    22321 cgtttcgatg cgacagattg aataaaagat tcatctactg aattgaaatc catagatttc
    22381 aataaaccat ctctacgtaa caatccgtta gaattctcgt ttaggcctct ttgagacggc
    22441 gttcctggat cagcgaaata aatggcaata tcattggcat tactgatctg tttccaattt
    22501 gaaaattcct ttccacaatc aaaagtgatg gatttgaata gatttttcgg tacagattca
    22561 aaccaatgat ttaatttgtt ttcaatatca atcgcttgtc taccacacgg tttcaatgtg
    22621 atgataactt ttgataatcg ctcaactaag gtaattacag cacttttatg tttcaaacct
    22681 acgatagtgt caccttcaag gtgaccaaac tcatttgaga attggctata atctttttca
    22741 cgttcatgaa tagagcgacg gaaagcttgt tttccacgtc tttcttgatg tccatttggt
    22801 ttacgcttgc ctttcatcgg taagtcagaa ggattaaata gccccttttt gaacatacga
    22861 taaatggtac gagcagaaca acgaatagga aacgctgcac gaccaacaat cacatcgggt
    22921 gtccatcctt gaacaacctt tctttgaata tattctgatt gttcctcagg taaaacaagc
    22981 gggcgtctac cacagtttga tttgtttttc ttatactgtt gatagtactc taaggctgat
    23041 tttccttgct tgaagaacag gtatacttta tggatcgttt gtcttgaacg attcaagaaa
    23101 tgcgcagttt tcgcaacaga ttgatttatt ttgaaataag actctatcat tacaagttca
    23161 tccgttgtaa gatgggtata ggtcatttgt actcactcct tataattctt tggacggaac
    23221 tattttgagt gtagcacaaa tgactttttt agttgtctag cttaatttta caatcggcgt
    23281 atataaaaat cccaagtaaa tacataaaaa ataaaaaaat tgaaactttt agcgcacctt
    23341 gtacgtcata ttaagtgagg cagaaaggag tggagaatga tttttactgg atataagaaa
    23401 aaacggcaga tcgaaaaata ccgtcacctg ctgaatggac tcatcgaaac tgattatcaa
    23461 aaaatgttcc gtatcacgat gaattacgta catgataaag aagaggcctt agatgtgatg
    23521 caagatagtt ttcataaagc cctgaactat tttgaaaaag aaaaagagat cgagcatttt
    23581 agtgcatggt tttatcggat attgatccgc acggcattag atagttggcg cagacaaaaa
    23641 aggacgcctg tggacttatt tgaaattcaa gatatccccc aacagttacc tttagaaact
    23701 tccgttgcag agctccatat gattttagat aaaatagaca gtccggaaaa agagattctg
    23761 atcctctact tttttgaagg atttcgtttg aaagaaattg cgactatctt ggatatgaat
    23821 gaaaacacag ttaaaacgaa aatgtaccgc tcattgaatg ctttacgtca aatcctagaa
    23881 tgaaggagag aagagtgcca tgtattccca aaaagaaaat ttaaaaaaac taaagaaaac
    23941 ttataaagaa caagcaattc ccaaagggct taaagaggag atccgccagc gttttattat
    24001 cgaagagcaa tcatttataa aagcaaagcg gagaaagaga cgtctccaag ggttcggagt
    24061 atctttggct atcgtaatga ttacaacaag tagccttctt tttaatagtc aagttcgaag
    24121 ctttgcagaa gatttaccca tcttaggatc agtcattgaa ttgatcttag gcgaacgctt
    24181 tactgatcga tctgaaaaaa tcgatattca ggtaccgaag atcaatacac aaaatgagaa
    24241 agaaaataaa acaattcatg gattaaatca aaaatacttt cgcgaaggac aagcagattt
    24301 tgaaaaagct gaaaaagaat atggaaattt tgaaacggat cattaccaag ttttgggaga
    24361 ttatcagaaa atcgtagacg ataaccggtt tctcgtgatt gaaaggcaga tcacccaaac
    24421 agcagcagat agccatgttg aaaaaagata cgacacaatc gacaagaaaa acagtgtcca
    24481 gctctcactt ccacttttat tcaaagatga cacctatcta tctgttttga caaaagaagt
    24541 caaacggcag atggccgaac aagtcaagga agatccatct aagtactatt ggactgagca
    24601 agatattcaa gaagggacga tagaaaaacc aacacttgtt acgcctacac gaagttttta
    24661 tttgaacaaa gagcatcaat tagttttgac tttttctcaa tacgaaattg ctcctggtta
    24721 tatgggtaca ccagaatttg tcattcctaa gtccgtgaca aaaacgattt tggcatcgga
    24781 agattatttg gatcattaga cgagaagtag ttgcgactag cagtttcaat tttcaagtaa
    24841 gtacaagtaa ataataagaa gagaaaaaga gaaagaagag atatagtgaa aagaaattat
    24901 ttagttatca gtattattgc tttatccgtg cttttgtttt tcgaaattac ttcggtggcc
    24961 tccttgatca ccgaagtaaa aggacaaaca cactcttcgg agcattcgtc acaatctttc
    25021 aaacaatcag aacaatcaac cattaaaagc caatcaactg aaacagctaa cgtcaaagaa
    25081 gccattcaag aatcagcgga aaaggtacaa agtcagtcat cacaatcaac acaagacagt
    25141 acaaaaacga gccgagatcc ggctgtcaaa agcgtcgcaa tcagctttga tgatggacca
    25201 ggagcgacca caacaccgca gcttctgcga atcttaaaag aaaaaaacgt gcatgccact
    25261 tttttcgtct taggagaaaa tacggcgcag catcctgaaa tcgtgaagca gacagctgaa
    25321 gcagggcacg agattggcaa tcacacatac gatcatcaga atctggccac tctttctgcc
    25381 cagtcgatga cggaggaagt gacgaaagca gacactgaga tcaaaaaagt cactggcaaa
    25441 acccccactt ttgttcgtcc gccctatggt tctgtcacga atgtaggagc aacgatcatc
    25501 cagcgaccga tcatcgaatg gtctgttgat tcagaagatt ggaagacaag aaatcctgat
    25561 ttgattttgc agaagatcca agcaacagta tacgatgggg caattatttt attccatgat
    25621 atctatcctg aaacgattcg tgccgttcct caagtgatcg attatctgaa agagcaaggg
    25681 tatcgcatca cgacagttgg ggatttatta gggcatccaa cggctgtcga gaactattat
    25741 ggcagaaacg atcatcggcc agttcagtaa gtgagacaag tatctttaat aaataacaca
    25801 ttttaataag tagttgaaca cataactacc ttacttgcaa aggcttaaca atatctatac
    25861 aggtacaaca aaaaactgtc caccaatttt ctttggcgga cagttttcac ttaaaaataa
    25921 agaagcaact attcgtaaat tatgctaaaa tagcagtaga acgcgtcctg agggaatcga
    25981 acccccgtct caagaaccgg aatcttacgt gatatccact acactaagga cgcaagcact
    26041 gggataattg taactaaaca gataaaaaat tacaagggga ttggacaaaa aaatgaaatt
    26101 tgagcaaaat ttttcacaaa aacagcagca gacacagaaa ctagccatga cgcaacagtt
    26161 gcaacagtcc atccaagttt tacaatataa cagtgaggag ctgttggcat tcgttgagaa
    26221 tcaagcaatg gaaaatccat tggtggaagt tgttgagccg gaatggcagc cagattacat
    26281 aaaagcttct tcatcttctt atgaaggaga agagacaaat tacctaaatc agattcctga
    26341 tacaaaaggg tctttatttg attcattgat cgaacaagtc catttgaatt accgagatac
    26401 ttttttgagg aaaatcgttc tttatcttgt agaatacatc gatttgaacg gatttttgac
    26461 gattagttta gaagaagccg gaaaagagat tggagctacg cctatccaaa tgctcgatgc
    26521 gttgactctg attcagcaat tagaaccagc tggagtaggt gcacgtagtt tgcaagaatg
    26581 tctcatgctt caaacagaac gagatgatta tgcgccggaa ttagcttata tcgtgttgga
    26641 agaatgtttt gaggaactgg tggagcgaaa atggaaagaa atcgcacaac gtttttctgt
    26701 tgatctacat gctgttcagc agatattcga ctatttacaa acgttgagtc caagccctgg
    26761 ccggattttc gaccgtagca gcgaattgta cattattcct gatgtccggg tattagtgga
    26821 cgacgataaa aatgtacaag ttatctcaaa ccgaaaaaat cagccaaata tacgctttca
    26881 agagagttac ttcaaacaaa tgtcacaaca agcagataaa gaaactgaaa attatttaaa
    26941 agaacggaaa caggaattcg aatggttgaa gaaaacgatc ctacaacgtg gggatacaat
    27001 tttgcgggtt gctcaagtca ttgtttcccg gcaaaaagct ttctttatcg acaaggaacg
    27061 tccaatcaag cctcttacgt taaaggaagt tgcaactgag atagatgtgc atgaatccac
    27121 ggtcagtcgt gcagtcaacg ggaaatattt ggaaacagac ttcggtgtat ttgaattaaa
    27181 aaaatttttc actacacgaa ttcctacgaa tagtacagaa cagacagaag acctttcagc
    27241 agatacagcg aagaaaaagc tgcaggaatt agtcgatcaa gaagacaaaa acaagccgtt
    27301 gtctgaccag aaattggttg aattgctgaa aaaagatgag atagcgatct caagacgaac
    27361 agtagcaaaa tatcgtgatt tattaggaat cccgagttca tcaaaaagaa aacgttatga
    27421 taactaatca gtcaaaagac tagaaaaagt atttcacttt ttctagtctt tttagttgaa
    27481 aaacgagagc aaaactgtta gacttacagt cgtgaggttg agatttccct ttattgatgt
    27541 gaaattcgaa tagttaattt ctttttttat cgtatatggg tcgttttaag tctacattgg
    27601 acgtaaagtg tccaactaag gagatcgcta tgctggatga aataaaaatg attgaatctg
    27661 ttgcaccaga tattatcgaa gtgttgcaag aacgctataa aattttacga aatatctatt
    27721 ggatgcagcc gattgggcgt cgaagtttat cggaaagtat ggggatcaca gaacgagtct
    27781 tacgaacaga aacagacctt ttgaaaaatc tgaatttgat cgacacctca aaaagcggta
    27841 tgacgctgac aactaagggc gaagaagttt atcaaagcct tgaaaacttc atggatcagc
    27901 ttttaggtac gcatcaaacc gaacaacagt tggcagaata ttttggtaac caacgctgta
    27961 tcgttgtttc aggaaacagc gaagagcaga ccaaagtggc tgatgcattc ggcgaagcgc
    28021 tgagcgaagc cttagaccga ctacttccag aaggcgagaa tatcatcgct gtgatgggag
    28081 gaactactat ggctgtggta gccgaacaat tgtctaatct ggaaaataag aaacggcata
    28141 acctgtttgt ccctgcaaga ggcgggattg gtgaagcgat aactgtacag gcaaattctg
    28201 tcagtgcaag aatggctgct aaagcaaacg gtaaccaccg cgcactttat gttccggaac
    28261 aattaagtct ggcaacttac aattctttac tcaatgagcc atctatccaa gaagtcttaa
    28321 acttgattag tgaagcgaat tgtgttattc atagtattgg gcgtgcatta cacatggcag
    28381 cacgtcgtaa gatgactgaa aaagaattag tcatgctgaa acaagcaaat gctgttgcgg
    28441 aatcttttgg ctactttttc aatgaaaaag gtgaagtcgt ttataaggtc cctagaatcg
    28501 gcatagaatt gcaagatttg gaaaaagtcc cgatcatcat ggcaatcgcg ggcggaaaat
    28561 caaaagctaa agcaattcga gcttatatga aaaatgcacc aaaacaaacg tggctcatca
    28621 ctgatgaagc tgctgcaaat gagattttaa aaggggtaac cctttaaaat aaatattttt
    28681 ttgattttca taaggaggaa atcttaaatg acagttaaag taggtattaa tggttttgga
    28741 cgtattggac gtctagcatt ccgtcgtatc caagatgtag atggaatcga agtagtagca
    28801 atcaacgact tgacagatgc taaaatgttg gcacacttgt taaaatacga cacaactcaa
    28861 ggacgtttca acggaacagt tgaagttcat gaaggttcat tcaacgtaaa cggaaaagaa
    28921 gttaaagtat tagctaaccg caacccagaa gaattaccat ggggcgaact aggcgtagat
    28981 atcgttctag aatgtactgg tttcttcact tctaaatcag cagctgaaaa acatttaaca
    29041 gctggtgcta aacgcgttgt tatctctgct cctggcggaa acgatgttcc aacaatcgtt
    29101 tataacacaa accacgaaac attaactggt aaagaaacag ttatctcagg tgcttcatgt
    29161 actactaact gtttagctcc tatggctaaa acattgaacg acaaatttgg tgttgttgaa
    29221 ggattaatga caactatcca cgcttacaca ggtgaccaaa tgactctaga cggacctcat
    29281 cctaaaggtg acttccgccg tgcacgcgct gctgctgcaa acatcgttcc taactcaaca
    29341 ggtgctgcta aagctatcgg tttagtaatt cccgaattga acggtaaatt agacggagct
    29401 gctcaacgtg ttcctgtacc aacaggttca ttaacagaat tagtaacagt tcttgaaaaa
    29461 gaagtaactg ttgacgaaat caatgcagca atgaaagaag cttcaaacga atcttacgga
    29521 tacaacacag acgaaatcgt ttcttcagat atcgttggta tgacttacgg ttcattattt
    29581 gatgctacac aaactaaagt aatgacagtt ggcgacaaac aattagttaa aactgttgct
    29641 tggtacgaca acgaaatgtc atacactgca caattagtac gtactttaga atacttcgct
    29701 aacttataag atagacaaac gtacaagtgt aaattataga catgtacatt taaaagcggg
    29761 gaagcaatcg cgcttcttcc gctttttttg ataataagtt tattcaggag gaaataaaat
    29821 ggctaagaaa acagtgaaag atatcgattt aaaagacaaa aaagttcttg tccgtgttga
    29881 cttcaacgtt cctttgaaag acggcgtgat cactgacgac actcgtatca aagcagcatt
    29941 gccaacaatc aactacgttc tagaacaagg cggaaaagca atccttttct ctcaccttgg
    30001 acgtgtaaaa acagaagaag ataaagaagg aaaatcttta gctccagttg caaaacgtct
    30061 aggcgaatta ttaggtaaag aagtaacatt cgtacctgaa actcgcggag aacaattaga
    30121 agaagcaatc cgcaacatga atgacggcga cgttgtcgta ttcgaaaata cacgatttga
    30181 agatattgac ggtaaaaaag aaagcaaaaa tgatacagaa ctaggtaaat actgggcttc
    30241 attaggcgat gtatttgtta acgatgcttt cggtacagct caccgtgcac atgcttctaa
    30301 cgtaggtatc gcttcaactg gtatcccaac agttgctgga ttcttgatgg aaaaagaaat
    30361 caagttcatc ggtgaagcag tagaagaacc aaaacgacca atgattgcaa tccttggtgg
    30421 cgcaaaagtt tctgataaaa tcggcgtaat cgaaaacttg attcctaaag cagataaaat
    30481 cttgatcggt ggcggaatga cttacacatt ctacgaagca aaaggaatca agatcggtaa
    30541 ctctctagta gaagctgata aagtggaact agcaaaagaa ttaatcgaaa aagcaggcga
    30601 caaacttgtc ttgccaatcg acaatgtctg tgcaccagaa ttttcaaatg atgttgaaac
    30661 acaagtaatc gaaggagaca ttcctgatgg tttgatggct ttagacatcg gcccagcatc
    30721 agtaaaatta tttgctgata cattacaagg tgcaaaaaca gttgtttgga acggacctat
    30781 gggtgtattt gaaatgagta actttgctaa aggtacaatc ggtgtttgtg aagcaatcgc
    30841 taacttagaa ggcgcaacaa caatcatcgg tggcggagac tcagccgcag cagcagaaca
    30901 attaggattt gctgacaaat tcacacacat ctcaacaggt ggcggcgcaa gcttggaatt
    30961 gcttgaaggt aaagaattac caggactagc agcaatcaac gataaataaa cgaccaagtt
    31021 tgctcaattc tgacgagtaa tcgaacaaaa ggtgaaggga gttttcatga ctccctagcc
    31081 atttttcaat aaattaagga gtgtgttcct caatgcgtaa acctattatc gcaggtaact
    31141 ggaaaatgaa caaaactgca tcagaagcaa aagaatttgc tgaagcagta aaaaacaaaa
    31201 tcccttcaaa tgacaaagtt gattcagtca tcggatctcc tgcattattc ttacaagaat
    31261 taatgacatc agctgaaggt acagaactaa aaatctcagc acaaaactgc tactgggaaa
    31321 actcaggtgc gttcactggt gaaacttcac cagcagctct tgctgatcta ggtgttcatt
    31381 atgtgatcat cggacattca gaacgtcgtg aatatttcca tgaaacagac gaagacatca
    31441 ataaaaaagc gaaagctatc tttgcaaatg gtatgacacc aatcctatgc tgcggcgaat
    31501 ctttagaaac ctatgaagca ggcaaaactg ctgaatggat cgaaggacaa attacagctg
    31561 gtttagctga tctttcagct gaacaggtgt caaacatggt tattgcttat gaaccaatct
    31621 gggcaatcgg tactggtaaa tcagctgacg cacaaatcgc tgacgaaatt tgtggagttg
    31681 tacgtcaaac agttgaaaaa ctttatggaa aagaagtatc agaagcagtt cgtatccaat
    31741 acggcggttc tgtaaaacca gaaaatattg ctgaatacat ggcaaaagaa aatgttgacg
    31801 gtgctttagt cggtggcgca agcttgcaag cagattcatt cttgtcattg ttagacgctg
    31861 taaaataatt gttgaaaatt tgcgaaaaat gcaagaaatg tttgcactaa tgagcaaaat
    31921 taactaaaat cgtatatgag ggaaatccct taggaaaaaa caaactcaaa ggagagacaa
    31981 aacatgtcaa ttattactga tgtttacgct cgcgagatct tagactcacg tggtaaccca
    32041 acaatcgaag tagaagtata cacagaaagc ggagcttttg gccgcggaat ggttccttca
    32101 ggtgcttcaa ctggtgaata cgaagcagtt gaattacgtg acggagacaa agctcgttat
    32161 ggcggtaaag gtgtaactaa agctgtcgac aacgtaaaca acatcattgc tgaagcaatc
    32221 attggctacg atgttcgtga ccaaatggct atcgacaaag ctatgatcgc tttagacgga
    32281 actcctaaca aaggtaaatt aggtgcgaac gctattttag gtgtttctat cgctgttgca
    32341 cgtgcagctg ctgactacct agaagtacct ttataccact acctaggcgg attcaacaca
    32401 aaagtattgc caacaccaat gatgaacatc atcaacggcg gatcacatgc tgataactca
    32461 atcgacttcc aagaattcat gatcatgcct gttggcgctc caacattcaa agaagctcta
    32521 cgtatgggtg ctgaagtatt ccacgcatta gcttcaatct tgaaagctcg cggattagca
    32581 acttctgttg gtgacgaagg tggatttgct ccaaaccttg gttcaaacga agaaggtttt
    32641 gaagtaatca tcgaagcaat cgaaaaagct ggctatgtac ctggtaaaga cgttgttctt
    32701 gctatggatg ctgcttcttc agaattctac gacaaagaaa aaggtgttta cgtactagct
    32761 gactcaggcg aaggcgaaaa aacaactgac gaaatgatca aattctacga agaattagtt
    32821 tctaaatatc caatcatctc aatcgaagat ggattagacg aaaacgactg ggacggattc
    32881 aagaaattaa ctgacgtatt aggcgacaaa gttcaattag ttggtgacga cttgttcgta
    32941 acaaacactc aaaaattatc tgaaggtatc gaaaaaggaa ttgctaactc aatccttatc
    33001 aaagtgaacc aaatcggtac attaacagaa actttcgaag ctatcgaaat ggctaaagaa
    33061 gctggctaca cagcagttgt atctcaccgt tctggtgaaa cagaagattc aacaatctct
    33121 gatatcgctg ttgcaacaaa cgctggccaa atcaaaactg gttctctatc acgtactgac
    33181 cgtattgcta aatacaacca attactacgt atcgaagacc aacttggtga agttgcagaa
    33241 tacaaaggtt tgaaatcttt ctacaactta aaaaacaaat aattcttttc taatgaagat
    33301 acatgatcga ctattgaaag atagtcgagt atgatcactt agttaagtaa tcgaactctc
    33361 tacttgctga aatatcagca ggtagagagt ttttttgcaa ctagcttctg tatatgatgt
    33421 ttacaaagaa tggtacaaga aaagatcatc cttgaaaaag gaaagctaac aataactagc
    33481 atcatataag agcttttaag gatagaacag tagaatagga atctatgggc aagtcctctc
    33541 gtataaa
//
LOCUS       DACCXO010000020        31343 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.021, whole genome shotgun sequence.
ACCESSION   DACCXO010000020 DACCXO010000000
VERSION     DACCXO010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.021"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            30..950
                     /locus_tag="IVY70_000896"
     CDS             30..950
                     /locus_tag="IVY70_000896"
                     /inference="COORDINATES: protein motif:HMM:NF012744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7958046.1"
                     /translation="MKTDITISFGQSCNLVNVLSKRDDKVYVGIRSYDWLFQYIFHYY
                     VDKFVYKKADAVVSVSQLIKRDAENIFNFNKDKSYFLYNPYDLELINNKSKEKITEIE
                     VPTDKKIIVTVGRLEDQKGLYHLIKAISMLKNKDNVVVYILGHGTKKELLENLIKAYQ
                     LQNVIFLLGGQSNPYKYMSRADLYVMPSITEGFPNALVEAMSTETAVLCADCKSGPRE
                     ILSNRTLDTICENIEYAESGILVCPMTDSRNYSPQYEECDKNLANAIEELIENDNLRN
                     KYAKAAKKRAELYSYDAFEHNLLQIMKTFN"
     gene            988..2079
                     /locus_tag="IVY70_000897"
     CDS             988..2079
                     /locus_tag="IVY70_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP7958047.1"
                     /translation="MLKKNTSLQNKTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFLFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
     gene            complement(2147..3625)
                     /locus_tag="IVY70_000898"
     CDS             complement(2147..3625)
                     /locus_tag="IVY70_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAP7958048.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRVESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
     gene            complement(3891..4639)
                     /locus_tag="IVY70_000899"
                     /pseudo
     CDS             complement(3891..4639)
                     /locus_tag="IVY70_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
     gene            4782..5732
                     /gene="pfkB"
                     /locus_tag="IVY70_000900"
     CDS             4782..5732
                     /gene="pfkB"
                     /locus_tag="IVY70_000900"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP7958049.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQKKQIQTEFIEVPGMTRINVFTQVNQTREE
                     YKLVNQGPEIPQAAVHNFLNQIRALQAEDYLCVSGSLPRGMSPKVLVEISRICQEKEI
                     HLIIDSSDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENV
                     LLSLGEKGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLVGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            5714..7177
                     /locus_tag="IVY70_000901"
     CDS             5714..7177
                     /locus_tag="IVY70_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7958050.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTILADNEAVDTLESETISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMLKTVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     SGGTGFLVGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVVFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPINKAAYVTGTALLAQGN
                     TTFMAGVSVACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEEGRAGGLS"
     gene            7174..7632
                     /locus_tag="IVY70_000902"
     CDS             7174..7632
                     /locus_tag="IVY70_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP7958051.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIAHDEKEVYRK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIVTLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            7629..8603
                     /gene="lacD"
                     /locus_tag="IVY70_000903"
                     /pseudo
     CDS             7629..8603
                     /gene="lacD"
                     /locus_tag="IVY70_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
     gene            complement(8782..9732)
                     /gene="yidC"
                     /locus_tag="IVY70_000904"
     CDS             complement(8782..9732)
                     /gene="yidC"
                     /locus_tag="IVY70_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP7958052.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10170..10445)
                     /locus_tag="IVY70_000905"
     CDS             complement(10170..10445)
                     /locus_tag="IVY70_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP7958053.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            10553..11317
                     /locus_tag="IVY70_000906"
     CDS             10553..11317
                     /locus_tag="IVY70_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP7958054.1"
                     /translation="MKEIQSAKNPKIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            11440..11943
                     /locus_tag="IVY70_000907"
     CDS             11440..11943
                     /locus_tag="IVY70_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7958055.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            12351..12998
                     /locus_tag="IVY70_000908"
     CDS             12351..12998
                     /locus_tag="IVY70_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP7958056.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALATEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMIYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            13161..14429
                     /locus_tag="IVY70_000909"
     CDS             13161..14429
                     /locus_tag="IVY70_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP7958057.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(14456..15655)
                     /locus_tag="IVY70_000910"
     CDS             complement(14456..15655)
                     /locus_tag="IVY70_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP7958058.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYAWQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(15774..17081)
                     /locus_tag="IVY70_000911"
     CDS             complement(15774..17081)
                     /locus_tag="IVY70_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP7958059.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            17552..18670
                     /locus_tag="IVY70_000912"
     CDS             17552..18670
                     /locus_tag="IVY70_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958060.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            19054..19374
                     /locus_tag="IVY70_000913"
     CDS             19054..19374
                     /locus_tag="IVY70_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958061.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGYHPV
                     IYEV"
     gene            19826..20026
                     /locus_tag="IVY70_000914"
     CDS             19826..20026
                     /locus_tag="IVY70_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7958062.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            20173..22962
                     /locus_tag="IVY70_000915"
     CDS             20173..22962
                     /locus_tag="IVY70_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7958063.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFIYVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGTGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMTTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            23009..23536
                     /locus_tag="IVY70_000916"
     CDS             23009..23536
                     /locus_tag="IVY70_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7958064.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            23557..24747
                     /locus_tag="IVY70_000917"
     CDS             23557..24747
                     /locus_tag="IVY70_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7958065.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFLKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            24787..26085
                     /gene="asnS"
                     /locus_tag="IVY70_000918"
     CDS             24787..26085
                     /gene="asnS"
                     /locus_tag="IVY70_000918"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP7958066.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            27262..27936
                     /locus_tag="IVY70_000919"
     CDS             27262..27936
                     /locus_tag="IVY70_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7958067.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            27933..28274
                     /locus_tag="IVY70_000920"
     CDS             27933..28274
                     /locus_tag="IVY70_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958068.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            28304..28663
                     /locus_tag="IVY70_000921"
     CDS             28304..28663
                     /locus_tag="IVY70_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP7958069.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            28866..29045
                     /locus_tag="IVY70_000922"
                     /pseudo
     CDS             28866..29045
                     /locus_tag="IVY70_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            29187..31268
                     /gene="cadA"
                     /locus_tag="IVY70_000923"
     CDS             29187..31268
                     /gene="cadA"
                     /locus_tag="IVY70_000923"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP7958070.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
BASE COUNT        10103 a         5382 c         6572 g         9286 t
ORIGIN      
        1 tttataaaat gaaaagtgtc aaaaaaaaat tgaaaacgga tataactata tcttttggac
       61 aaagttgtaa tttagtaaat gttttatcaa agagagatga taaagtatat gttggaatcc
      121 ggtcctatga ctggctattt caatatatat tccattacta tgtagataaa tttgtatata
      181 agaaagcaga tgctgttgtg tctgtcagtc agttaattaa acgtgatgct gaaaatattt
      241 tcaattttaa taaggataaa agctattttt tatataatcc atatgattta gagttgataa
      301 ataataagag taaagaaaaa attacagaaa tagaggttcc aacagacaaa aaaattatag
      361 tgacggttgg tcgattagaa gatcaaaaag gtttatatca tctaattaaa gctatatcta
      421 tgttaaagaa caaagataat gttgttgtgt acatcttagg acatgggaca aaaaaagaat
      481 tattagaaaa tttaataaaa gcatatcaac tacaaaacgt aatatttttg ctaggtggcc
      541 aatcaaatcc ttataaatac atgtcacgtg cagatttata tgtcatgcca tctataactg
      601 aaggttttcc taatgcatta gttgaagcaa tgtctacaga aactgctgtc ttatgtgcag
      661 attgcaaatc tgggccaaga gaaatattat cgaatagaac gctagacacg atttgtgaaa
      721 atatagaata tgctgaatca ggcatattag tatgtccaat gacagacagt agaaattatt
      781 cgccccaata tgaagaatgt gataaaaatt tagcgaatgc tatagaagaa ttgattgaaa
      841 acgataattt gaggaataag tatgcaaaag cagcaaaaaa gagagcagaa ctatatagtt
      901 atgatgcctt tgaacataac ttgttgcaaa ttatgaagac atttaattaa ttcatatgat
      961 gaaataaaaa taagggagta taactagatg ctgaaaaaaa atacatcttt acagaataaa
     1021 acaatattaa taactggcgc agccggtttt attggatcaa atttggttct tgagttatta
     1081 cgtactcagt catcaataaa aatcattggc attgacaata tgaatgatta ctatgatgtc
     1141 tctattaaga actatagatt atcagagata gaaacggagg caaagaacca tccaggatct
     1201 acttggaatt ttatcaaagg ttctattgct gataaagcaa caattgattt cctctttgat
     1261 aattataaac caagtatcgt ggtgaattta gcagctcagg caggagtccg ttattcaatt
     1321 actaatccag atatgtatat cgaatctaac ataataggat tctataatat tcttgaagct
     1381 tgtcgtcatt catatgataa tggagaatct ggtgtggaac atttagtgta tgcctcatcg
     1441 tcttccgttt atggtactaa taagaaaatt ccttattcta cagaggataa agtagacaat
     1501 caagtatcgt tgtatgcagc aactaagaaa agtaatgaat caatggcaca tgcatattct
     1561 aaactttaca atattccgtc tacagggcta cgtttcttta cagtatatgg tcctgctggt
     1621 cgtccagata tggcttattt tggttttact gataaacttc gtgctggtga taatatccaa
     1681 atattcaatt atgggaattg caagcgtgac tttacctata tagatgatat tgttgaagga
     1741 gtaaagaggg ttatgcagtg cgctccagag aaaattgatg gggaagatgg gctaccaatc
     1801 ccaccatatc atatttataa tattggtaac aatacgccgg aaaacctact agattttgta
     1861 gatattctac aacaagagct aattgcagca ggggtgctac cagaagatta cgactttgaa
     1921 tctcataccg aactagttcc aatgcaacca ggagatgtgc cagttactta tgcagatact
     1981 agtgcgttag aacgagattt tgaatttaaa ccaagtacaa gtttgcgtga cgggcttcgt
     2041 aaatttgcgc agtggtataa gtctttttat gaagtataat tgtatttttt tacttataaa
     2101 agtttataga aacacctcag acactacaaa tctgaggtgt tattttttaa tctcgttgaa
     2161 ataaatctct ggtatatact ttttctttta catcatcaag attactatcg taacgattag
     2221 caataatact gtgactgata tttttaaaat catctaaatt atttattact tttgaaccaa
     2281 aaaatgtagt gttattttca agtgttggtt cataaattac aatagttgcc ccctttgctt
     2341 taattcgctt cataactcct tgaatagatg actgacggaa gttgtctgaa ttactcttca
     2401 ttgtaagacg gtatacacct atgactacct ctttttcttt ttctgcattc cacgaactgt
     2461 tagcctcata cgtgccagca agttctaaca ctcgatctgc tataaagtct tttcttgtac
     2521 gattactatc gacaattgca cttatcatat tctgaggaac atcgttatag ttagtgagaa
     2581 gttgttttgt atctttcggt aaacaatagc ctccatatcc aaaagatgga ttattataat
     2641 gtgtgccaat tcttggatct aagcaaaccc cgtgaatgat ttcttgtgta tttagtcctt
     2701 tcatttctgc ataagtatcc agctcattga agtaagatac tcgaagtgca aggtaagtat
     2761 tagcgaataa tttaactgct tcagcttctg tgaatcccat gaaaagagtt tcgatatctt
     2821 ttttgattgc tccttcttgg agtaaatctg caaaagtgtg agaggcttct cgtaaagttt
     2881 tgtcagtttg gtccgtgccg acaataattc gcgaaggata tagattgtca tataatgcct
     2941 tagattctct gagaaattct gggctgaata gaatattttt agtattgtat tttttacgaa
     3001 tactttcggt atagcctacg ggtaccgtcg acttgatgac cattattgcc tttggattga
     3061 cttctaacac caatttgatt actgactcta cagcagaagt atcaaaataa ttttgtttac
     3121 tatcataatt tgttggagca gcaatgacaa caaaatcggc ttctctatag gcggctgcac
     3181 catcagtcgt tgcaattaaa ttaagatttt tttcagctag atatttttca atgtaatcgt
     3241 cttgtattgg agatttctta ttgttaattt gttttacttt ttcaggaaga atgtctactg
     3301 ctgttacctg attgtgctgg gcaagaagag tagcaatgga aagacctacg taaccagtac
     3361 cggctacagc aatattcttc gactgatgtt ttgttctgtt tgatttattt gattcattta
     3421 taaatagatc tgttggttca aaatttagaa cttctgctaa tgattctaat tgttgtattg
     3481 acgggataaa atcgagagat tctactcgac taattaaggc acggttgata ccagtagcta
     3541 ctgcaagttg ttgttgtgtc attttcagag attttctaga atcaataatg atttgagcta
     3601 aacgtttttg agataacttt ttcatgatgc actccttttt ttgttgctaa taataacaaa
     3661 caattttttc ttctataaca gaatagctta ttattaataa aactgtcaat ttttttattg
     3721 attaaaaaga tattttttga ttttaagtat tttcaattat tttttgttac ttaaaataac
     3781 tttatagatt gtgaataact ttatacataa aaagtttacg tgagtccata caatataaag
     3841 aataaaaaat gtgatgtttt aaactgtaac caaccataat aaactgcttc tcaattctcc
     3901 ctcatacgca atacataggc gtcaagcagg attctcgcga tgatctggag aggcaagcgt
     3961 gaacgtactt cataatcagg gaaataagaa ccttctgatt tgaaacctac gagatttatc
     4021 tcgcaataat ctttgagtgt agaggaatga ttggctgtaa ctagaatcga accaatctct
     4081 gcatcatagc agttctttgc ggcggtgacg agttcttttg tctcgccgtt caaagaaaca
     4141 aaaataacgc tgtctttctt agttagtttc cgactgatgc ttttgataat ctctggatca
     4201 gtatgcattt gacaattttt tcctgttaat tgaagtttta cgagcatctc ttgaccaatc
     4261 atttctgaaa atcctcgggc aaagacaaaa acccggtcag taaatttgat tctttgtatt
     4321 gcatcttcaa tgttcccgat ttccaacata ttgatcgtac gtaccacttc ttgttcattt
     4381 tttagaatgg ctcgacggat acctttgtct actttatcta aaaagaaaaa ttgatgttct
     4441 tgttttctcc ttcttttaga tggtgcttaa acgaagtaaa tccttcatag ccttttttct
     4501 tcattgtccg cacaatggtg gctgttgaga catttgcttg ttcacttagt ttcacgatgg
     4561 agtagttagg gatctctagg atgtggtgtt cgataaagtt ccagaggtac tgttctgttt
     4621 cacttaattt gtgatccata ttctttcctt ctttcactaa aagttagtta aaaaatagca
     4681 cgatgtgttt ataaatgtaa aggttttctt tgtgttggaa cgattaagaa aacattttct
     4741 ctttcgcgta tttcataata gattcaagga ggtcattggt tatgtctatc tacacatgca
     4801 cgatgaattt ggcaattgat ctatttattg aaacagagga gatgcatcct tttatggtaa
     4861 accgtacaaa ggaggatgac attcaggcaa acggaaaagg agtaaatgtg tcactagtat
     4921 taaagatgct agatttccca agtacggcat taggtttttc tgctggtttc acaggaaaat
     4981 atatagaaga ctatttacag aagaaacaaa tccaaacaga gtttattgaa gtgcctggta
     5041 tgacgcgtat caatgtattt acacaagtca atcaaacgag agaagaatac aaattagtga
     5101 atcaaggacc tgaaataccg caggcagctg tccataattt tttaaatcaa ataagagcat
     5161 tacaagcaga ggattatctt tgtgtttctg ggagtttacc aagaggaatg tctccgaagg
     5221 ttttagtgga aatcagtcgg atttgtcagg aaaaggagat tcatttgatt attgatagca
     5281 gtgatcaaga ggtcatggat tgtttgccct atcgaccatt tttgctgaaa ccgaatgagg
     5341 aagaactttc ttcttggttc ggacgaaaaa tggttacaga tgaagattac tttgtttatg
     5401 gacaacgact ggtagagtca ggtgcagaaa atgttttgtt atcccttggt gagaaaggag
     5461 cgattctttt tacgaaagac agggtgattc gaggaaactc gccaaaagga aaagttgtca
     5521 atacagcatg tgcaggtgat gccatgttag gcactttttt agtaggatac atgaatcgac
     5581 gaccgcttga tgaaacgtta cgtaaaagca tagcagcagg cagctctact gcttttcgta
     5641 aaggactgac tgattttttg gatgtggagg aattaagtaa acaaatcaaa atcaaggaag
     5701 agaggttcga aagatgggaa aatatcaact aattgcagca accggttgtc cgacaggaat
     5761 tgcacacacc tacatggcgc aagaggcatt agagcaagca gctagaaaaa aaggcatcac
     5821 gatcaaagtg gagacccatg gacaaattgg gatcgagaat gaattaacgg ctgaagaaat
     5881 caaacaggca gatgctgtca ttattgccgc agacaaagat gtccattcag aaagatttgc
     5941 gggaaaacga gtcattgatg tttctgtcag tacaggaatc aaggatgcac aacggctgat
     6001 tgacgaagca ttggcaggaa aagggaccat cttagcagat aatgaagctg ttgatacttt
     6061 agaatcagaa acgataagtg gtgctaattt tggtcgcagc atctataaaa atttaatgaa
     6121 tggcgtctct catatgttgc ctttcgtggt agctggcgga gtattgattg ctatttcatt
     6181 tgccgtttgg ggaatctatt cttttgatcc agaccatgca caatacaatg ctacagcggc
     6241 gatgttaaaa acggttggcg atgcagcaat ggggatgatg gttccagttt tagcagctta
     6301 tattgcagaa gggatcgcga aacgtccagg gttagtcgtt ggatttgttg gtggaatcgt
     6361 ggccatgagt ggcggtactg gatttttagt aggtatttta gctgggttct tagcaggtta
     6421 ttttatctta tttttgcaaa agctactgac aattttacct aaacaactag atggcttgaa
     6481 ggcaatcttc ttatatccag ttattggtgt agtgtttatc ggcatcgtga tgtctttgct
     6541 ggcgggtcca atgaaggcta taaatgaagg aatgatgagc tttttagctg gatttgaaca
     6601 ttcgaatcca cttgttcttg gcgtgattgt tggttgcatg tgtgcgtttg atatgggagg
     6661 accgattaat aaggcggcat atgtaacagg tacagctctt ttggcacaag gaaacacgac
     6721 ttttatggct ggcgtgtcag tagcttgcat tgcaccaccg ttgattactg gttttgcaac
     6781 tttattgttt ggtaaatatt ttgatactaa tgatcgtaat gcaggtttgg ttaattttat
     6841 tttaggatca actcatatta cggagggagc tattccgttt gctgcgaaag acccgatccg
     6901 agtattacca acgatgatgt taggctcatc aattgctgct gtcttgacct atctgtttgg
     6961 tgtccaagta ccagccccac atggcggttt tttagtgtta cctgtagtga cgggaaaaat
     7021 ccaatgggtt acagcgattc ttattggttc gatcgttggt ggagtgatac taggtttgat
     7081 ccaaaagaaa agagtaactg ataaagacaa gaagatggat aataaagcag caaatcgtac
     7141 agttatcgaa gaaggaagag caggaggctt atcatgacaa tcattcatga agagaacatt
     7201 tttttacaac agccattaga tagtcaagaa gaagcattta agtttttagc aaagcaaaca
     7261 ttctgtttag gaatcgctca tgacgaaaaa gaagtatatc gaaaacttct agaaagagaa
     7321 aaagaaggta ctacaggaat aatgtcaggt tttgctatcc ctcatgcgaa gtctcaaaca
     7381 atcaacgaac ctagtatgat tatcgtcaca ctgaacaaag gaattgaatg gaacagttta
     7441 gatagacagt cagttgattt tatttttgcg ttatttattc cagattcaga agcaggaacg
     7501 actcatttga aattattgtc tctcgttgca cgatcattga tgcgaaaaga ggttacagaa
     7561 cagttgagaa aagcaaaatc aaaaacagaa atagccacaa tattaactaa agaaataggt
     7621 gaagatctat gaagaagatg agtatagaaa agcaggaacg tttgaagcga ttgacggatg
     7681 aagctggaat tattagcgcg ttagcaatcg accagcgtgg tgctttgaag aaaatgatca
     7741 accaatataa agaagctaca tcagaagaca tcatccaatt taaacagatg gtttctcaat
     7801 acctgactcc ctatgcctca gctattttac ttgatccaga atgcggcctg cctgcagcag
     7861 cgcttagaga gaaagaatgt ggcttgctgc tagcttatga aaaaaccggc tatgatacga
     7921 aaatgccggg aagattacct gatttattga atatttggtc ggtgaaaagg ttaaaagaag
     7981 ctggtgcgga tgcttgtaaa tttttattgt actatgacgt ggatgaacca gcagagatta
     8041 atgtgcaaaa acaaatagtt atagaacgga ttggttcaga atgcgatgca gaaggcatac
     8101 ctttcttttt agagatcctt tcttatgatg caactattgg cgatccgtcc tctcttgcct
     8161 atgcaaaagt aaaaccccat aaagttattg aagccatgcg agaattttca aaagaacgtt
     8221 atcaagtgga tgtattgaaa gtagaagtgc cagtgaatat gagatttgtg gaaggatatg
     8281 ggcaatagac ttgttatagt caagcagagg catccgcgta tttctatgaa caatcgaaag
     8341 tgactgaaca accgtttatt tttttaagtg cgggagtcaa tgctacgtta tttcaagata
     8401 cattacgttt tgcaaaacaa tcaggttcga cattcaatgg tgtcttatgt gggcgagcaa
     8461 catgggcgaa gggagtggca ccttttgtta ccgaaggcga agaaaaagcg agtagttgga
     8521 tgttagaaga aggaaaacaa aatattaagg aattaagtac actattaaaa gaaacagcaa
     8581 cacctgtcta cgttgataga taagaaaaag ttgtttcttc ataaaaagat gctgctgcaa
     8641 ttagatagcg atcaatcgat gattcaaatt atacattcac ttaaataaaa atatcgacta
     8701 gatttctttt tcaaaaatct tggtcgatat ttttaattgc taaaactgtt aatgaatttt
     8761 tattacactc ttatttacta tctacttccg cttctgctgc tttcctgcgt tgcggccttt
     8821 gttatttgta ctgttttttg gttgtttgat ttgttttgtt gcttgtttgt tgtcagctgg
     8881 ttttgctgat tctgtcacgt cttttcttgg agtgacgact tgttttggcg ggttttgttt
     8941 caactcttct gccacttgtg ctttcaagcg tggtttcatc ataacgtttg tgatgaatgt
     9001 ttgcagacag ctgaacacac caccgactac ccagtaaagt gtaacgccgg ctggcgcact
     9061 gatcgacatg aagacgatca tagcaggaga gacgatcagc atcgtacgca tcgtcttttt
     9121 ctgttcttca ggaataccga ttgttgaaat atatccttga agcagataag cgacccctgc
     9181 aatggccaca agcaccatac ttggtgagcc tagatcgatg ccgtaaaaag atgattcacg
     9241 gataccttct gtgtagcgag ccgtaaaata caaggcagag aagatcggca tttggatcaa
     9301 tagaggcaga cagccgattc cgccggtcat actcaatccg ttttcgcgat atacttgctg
     9361 catttctgct tgtgctgcca tttgttcttc acgtgttgtt gcagctttta gcttttgttg
     9421 tgctgcttca acttgtggtt ttaacgcttg cattttttca gattggatca ttgttttttt
     9481 cgattgactg atacctagtg gcaagatgat gatacgaacg atcacagtca ttgcaataac
     9541 tgcccagccg taagaccaat tgaaattctc aactagatat gtaatcgctt ggcccatagg
     9601 atggacgagt actcgataaa cgattcctga agtgtcaggg tttccgtttg aatccatccg
     9661 cacacagcca gataaaaaga gcaggatact gacaagtccg gatcccaata accagttttt
     9721 ccatttattc atagaatact tcctcttcta taatttttac acaaacttaa ctatactaaa
     9781 aaagcgcatg gaattcaatc aacttcatag aaatttcata taaatattct ttctttttcg
     9841 tagcgaaaaa ttttgaaaaa agacaaaaaa tcaagaaaga aatacttgga aagaaaagag
     9901 tgattccaag tgtttctttc ttatataaaa aactaaaaac ttttttgttc tgtctggaaa
     9961 atatgggtta agcaaattta ttgcaagtta tagatcagct tttttactac ctatctttaa
    10021 tacttgaggc aaagcgcctc aaaaacaacc tgatcgacgg tgttcaaaag tcagtttctc
    10081 ggtattaagt caaatttatg caaaaatgtg gaaagccatt ttcacaaaat tttccttaat
    10141 actcaaaact aagcgacctt ttcacaacct tatcccacca catcaaattt ctttcgttcc
    10201 tcaatcatcg gatcttcttg gacatccaca tactgcactc gtccacttgg gctcggagac
    10261 ttcttgactt cttcgataaa tttttggatg atgtcgtcat ttccgattgc ttcgattgtg
    10321 actgagccgt cgtcttcgtt ttttaccgtt ccggtcacgc ctaactgatc agcaaccatc
    10381 ttggtcatat agcgaaatcc cacgccctgg acacgtcctt ggacattcat tttaactttg
    10441 cgcatgttcg tcacctttct ttcctgtttt ataaatcaat gataccgatt tttttaggat
    10501 gaaacaagct caagcaccta ttccatgata taatcaggtg aggtgaagga atgtgaaaga
    10561 gatccaatca gcaaaaaatc caaaaatcaa agaattaaaa aagctacata aaaagaaata
    10621 cagagaagaa aaggcagaat atcttttgga agggttccat ctgatcgaag aagctgcgtc
    10681 gtctgatgca gaaataaaag aactttttgt gacacagaga ggtatgaaag aatggggaga
    10741 ctggctggct gtaaacaaag agatttctac ttttctagtt tcagatgagg tcatgaccgc
    10801 tatgtcagat ctacctacac cgcaagggat cttagcggtg atgacgttaa catctgaaga
    10861 tacgccagat ttttcaggag gctggctttt actagataat gtacaggatc ctggaaatgt
    10921 gggaacgatg atccgtacgg cagatgcgtt tggtatggct ggtgttttat taggtaaagg
    10981 aacggcagat atttatagta caaaggtgct gcgaagcatg cagggaagca attatcattt
    11041 gcctgtgtta caaaaagatt tggcagaagt gatccctgta atgaaaggca aagggtttac
    11101 ggtgtacggt acagaattaa atgaagaagc acatgcatta gacaaagtag aaaaaacaga
    11161 ggatttcgct ataatcatgg gaaacgaagg tcagggagta agtcaggaaa tcttgtcttt
    11221 gactgatcag aacgtatata tcccaatgaa aggaaatgcg gaatcattga atgtcggagt
    11281 cgcagcaggg atcgttcttt atcatttttc tttatgatta aggaactatt aagctttagc
    11341 tattttatct agagcgacta gaataatccg aacactggtg gtatacttag atcaatttca
    11401 gaagaaaatg aaattcaaaa aggtggcaga tcgataatta tgacagaaaa atggcgtgaa
    11461 gacgaagaat atatgtctta tgtacaagat cttttagaaa cagaagcagt gaaaaaatta
    11521 gcgaattaca cacagcatgt tcattccact cgtttggagc attccatcag tgtttcttac
    11581 tatagttatc tgctcgcaaa aaaatggggt ggcaatgcta aagcaacagc gcgtgctgga
    11641 cttttgcatg atctgttcta ctatgattgg cgtacgacga aatttgacga ggggacacat
    11701 gcgtatatcc atccaagaat tgctgtaaag aatgcagaaa aaatcaccga tttgtcggat
    11761 cttgagcgag atatcatttt gaagcacatg tggggtgcaa cgatcgcacc gcctaagtat
    11821 aaagaaggat atattgtgac attcgtggac aaatactgcg cagtcaaaga agcggctcag
    11881 cccatgtctg caagtatgcg taagagatgg caacgttact ttgggaaaga atcctcgatt
    11941 taacaggtaa aaccaataat agaccatatt gaggttgtga cagaagtggt cagcttcaag
    12001 aaataagaag gaatttacga aaattacttc tcaaattttt gtgaagtttc ggcttattac
    12061 cgcagatgca agcttttgaa caccattcat tcgaaacaag ggactttgac acgacttttg
    12121 tcacagtcct tttttctgtt tgcagttgac tatctgttca agatacggtt tacttttatt
    12181 tatcgaatga atcatgtaca tagaagaata agcattaaag ggggtgatga taagatgcaa
    12241 aacgtatctt atttaaaaga gtccgggatc ttcaggtaac acatactttg cagaagatca
    12301 cttttttcta caaagtatgc actttggtta gaagaaagga ctttaaaatc atggaacaaa
    12361 caataaaacc ataccaatat ttttttataa aaagagaagt agaacagtta ttaaatgcgt
    12421 actcatctgt caatgatcca aaaacggtac aaacagttca ggcactagca actgaaaaga
    12481 ttcgagacat attagaacac gaactgccag aaatcgatac atttttaacg acagttttag
    12541 acgtaaaact gacaaaagcg caagcagaac gagcattgga ggagctgaag caaatcgttc
    12601 agccgtttat gcagcctagc aaaccacaaa tcgagaaatt attccgaaaa gtgaaaaaac
    12661 tcaaacaacc agcttggaat gaaatcgact tgcaagaaca tacgtacatc ggctggaatg
    12721 atccaggtac gcagaaaaaa ttcatgatct attacgaaga tgaaaaatta aaagggatta
    12781 gtggtacttt atcaccaacg ataaataagg gaatctgtgc aatctgtcag aaaacatcga
    12841 atgtttcgct ttttctttct acaacgaaag caggttctga tggcacgtat acgaaaaagg
    12901 ggaattatat ttgtcatgat agtgaccaat gtaatcaaca gttaacgaaa ttggctcctt
    12961 tctatgaatt tgctgaacgt gtaaagaaag aacgataatt ttattgtttt ttacagaaaa
    13021 atatgctatc ttttaatgga agtgaatagg tgatggtgtt cgcctttaaa tgctaacgaa
    13081 agatagctaa tgacgcctac taaagtctgg ggacagcccc tttctttagt aggcgttttt
    13141 ttattgagga ggaatttttt atggaaaaac aattaccgag tccagtacgc attttagtgt
    13201 acatgctgaa gtggctggtg atcacagcga ttctcggtat attcatgggg agcttgtctg
    13261 cttttttctt aaatagcctg acagttgtga cagatatccg actatcacat ccttggctcc
    13321 tttatctact accagttagt ggtgcgcttt ttgcttatct ctatgcgtat cacggcggtc
    13381 tttctagtcg tggaaacaac ttagtgatcg atcaaggaaa tggtggagaa gaaaaaatcc
    13441 cgttgcgact tattccactt acactgtttg gaaccataac tacccattta tttgggggat
    13501 ctgttggccg tgaaggaaca gcagtccaaa tggggggagc gttagcagat aatacagcac
    13561 gtttgttccg attggataaa tcagaacggg aaatcttgat tatcagtgga atcagtgcgg
    13621 gttttagttc tgtatttggc acacctttag ctggaacatt atttggatta gaagtgttgg
    13681 caatcgggaa agtccgaaca gaagcgatct tccctagctt ctttgctgca ctgtttgcaa
    13741 atttcatgac agaaagctat ggtgtttctc atctacatta ccaaatgggt gtgattcctg
    13801 aatggagtat gttgctgttc ttcaaagttt ttgttgctgg gattgctttt ggtttgattg
    13861 gttgggtatt cagccgttcg atcgtctttt taaaagcgcg atatgcacaa tggattcctc
    13921 atcctgtttg gcgaaatacg ataggtggag ctgtagttgt ggcagcagca ttgattttgc
    13981 aaacacaacg ttatctcgga ttgagtcttc cgttacttca agaatcattt aatggacagg
    14041 cacatgtcta tgattttatt gggaagttat tcttcacagt tttgtcttta ggcgcgggat
    14101 atcaaggggg agaagtgaca ccattgtttg aaattggtgc aacgcttggt gcagcactag
    14161 cgcctttgct ccatgtttct gttccttttc ttgcaggatt gggctttatc ggagtatttt
    14221 ctggtgcgac aaatacaccg attgcctgct ttgtgatggg gatcgaattg ttcggttcac
    14281 aagcggcagt atacttcttt atgatctgct tgatcagctt tatgtgttca ggcaacagcg
    14341 gtatttattc tgctcagcaa gtagcggtca aaaaagggat actctttttg ccaatagtta
    14401 agagaatgaa agaaaaaaac aaggtgtgac atatgttttg tcacaccttg tttttttaga
    14461 aaagtaaagt aatcagcgat aagaatgcgg agcctgcgaa aagcaatccg caaatgatca
    14521 tatgtctttt tttgttcatc atcaagatgg cgatgtattc tcttaaaaac gcattaggca
    14581 agtagtagaa agctgttttt gcaccaatac catttgcttt caatttggct tgccacgcat
    14641 aaagaccggc tctaaaaatg tggtaattgt tggaactaaa gatcgcgcgg tatggccctt
    14701 tcatttgctg atccatgatt tcctttgaat agagcatgtt ctccaaagtg gttgtcgaat
    14761 tggtttcgac aagaatgtct ctttcaggga ttccttgttc catggcatat tgtttcatcg
    14821 caatggcctc tggtactttt tcgtctgctc cttgcccgcc agacatcaat aagatgggcg
    14881 gatttaatgt agcacggctt tgagcccgat aaaaagcaat tgccttattg attcgcttgg
    14941 ctagaagagg agaaactgtt tctccattga tcaacccagc gcctaacaca acgatgaagt
    15001 cttgattgta tcgaggccgg ttgaactgat acaagaatga gacagtcaaa aagttgtaga
    15061 atacgataaa tagatagatc aagatcaggg gaacgcagaa aaacaaagca tttatccatt
    15121 gcggcaaata ttttaacaag aaaaagtcaa aaacaagaaa cagtgtaaga aaaatggcta
    15181 gtatcaacgt taaaagatta gctaatgaat gcgattctct acgtaaaact gtgaccccgt
    15241 tccaaaaaag aaaaacaatc aatccataaa taccaaataa aaggacaagt agcaatggaa
    15301 tcgtgatcaa ggcgatcagt ccgccaagga aaatattttg cgtttcatac agcaagacaa
    15361 acaagtagat gccgaaactg atgaggaaaa cattgaataa aagaccattc agcagccttc
    15421 gtttttctgt gaaataagaa aaagcaaaga tactaaagaa caacaatgga atcaaaaaat
    15481 aaaaggtcga ataaaaaatt tccaacacga caaaataagt taagattacc gtaaatgtcc
    15541 acgtactgcc gtaaaagaac aaggaaggat ccttcttctg ttttcgccaa taaacgatag
    15601 agaatacccc aataagatag agaactaaaa agatcagatc tctggttaga ttcataaatt
    15661 caactccttt ttcttataca gtataacgaa aatagatgaa aaaagctgtt atttaaacta
    15721 gaaaaagcaa aaaaacatct ttccgatgga aagatgtttt tcttattgtt caattaaaac
    15781 aagttgccga aaaattctgc gacgcgtcgc cagccaatca cgaatatatt cgctttttcc
    15841 actgttttat ccgtaatgat ctctgttgaa ggtgcattgt tgtcttctaa atagcctaat
    15901 ttatcgtctg ccaaggtgat cgtagcggta ccgatttttg tacctttatc cataggtgct
    15961 tggatctcat tgtcagtgac ttgcttcttg tcaagtgttg gcgtgatcgt taatttgcca
    16021 gtatccatat cgtttcgtac ccatactttt acagggcttt taagcacaac aggaactgtt
    16081 gattcttttc cgtcttttac atcaatggtt tttagactag gaatacttgc gttagctttt
    16141 cctagctctt tttctgacca attgtcatag cagtagtcca tcaaacggtc tgtttcattg
    16201 aagcgggcac tcgggttttc cgcatggccg tccgcattca atacgacagt gatgatccgt
    16261 tgatcgtttt tagtgatcgt accaacgaaa caagcacctg caagctcagt cgtaccagtt
    16321 tttaatccgt ccacgccttc cttgtaatta acgaatcctg ggagcatcca gttccagttg
    16381 accatttcca ctggtgattg cgtattttcg ccaaacattt gggtcgtagt acttgataca
    16441 tccagtattt ctgggaaatc caagatcaaa tggcgggcaa cgatggccac atcttgagca
    16501 gacatttgat tttcgtcgtt ttctccagtg ccttctggtc ggttttcgcc aagataagag
    16561 ttgttcaatc ctgatgcatt aacgattgtt gcatctttga ttccccaatc tttcaattgt
    16621 tctttcattc gttcagtgaa tttagcttcg ctgccagaga ttttttctgc caaggccacc
    16681 attgaagcat tggctgactg gatgattgca gagtcgaata attctttgac agtgtaggtg
    16741 ttttctttat gcaaaggaac attcgataaa tctggggtga tactcaaatt ttctgcataa
    16801 tctgaaatcg aaactttatc atcccatgac agttttcctt cttttacttg atcaaggacg
    16861 atgtaaagac cgatgatttt tgtgattgaa gcgatcccca tggttttttc tccatcttga
    16921 tcataaagaa tcttaccgct ttgcgcatct acagcaaaag ctgcttttgc atttactttg
    16981 aacgtatctt cagcttgcac agcaacagct ggtagaaaag aagcaaggca aagaatgaga
    17041 ctagccaata ataggactag acgtgttttt tttatggaca tatccgatct ccttgtaatt
    17101 aatttgtttc gctacaaatt aaaattttat cagagagggg agagagtttc aatgtttctg
    17161 aaaaaacttt aggataagaa cgcgcgttat ggaagtttag cggtcgtttt tacgtacttt
    17221 tacaggaata ggctgtgttt ccgcatctaa taagcgggaa gcgctgaaag caaataaact
    17281 gataatactt aataaactga aaatcataat caaatgcata agatccacct cttttattct
    17341 ttttcttagt ttatcagcat ttcatgaaga atagttgaag actggggcta aataattgag
    17401 gaaattatga agataactta aaaaaatagc taaatttaaa accgtagaaa ccgttttatt
    17461 tttttagata cgttttattg tatggaaaaa tagtgttgtg gtagacttta gatagaaaca
    17521 aaggaatgat ttttttagga ggggtacgtt tatggcaaaa aaggataaag aattattgac
    17581 acaattgact tcagaaggcg ctaaaggacc attcgtcacg atcatgctga atacgcatgt
    17641 cgcccatcaa gatgtggaaa aagatcagat caaattcaaa aattttgcga aagaagcaaa
    17701 gaaacgtttt gagaaaaaat attccgaaca tgactggtcc ccgttccaag aaaaaattga
    17761 aaatttgtta ggagatcctt cgttttggcg tagtgcaact acgagtgtag cggtcattat
    17821 aactgctgac cagacctata tccatcgttt gagtatcccg gtagacgatc aatactatgt
    17881 aagtgatctt ccttatttac tggctattat caaaaatggg cagttcaact attcttatta
    17941 tttattggga ttgaaccgcg actccatgaa attgtatgag gtcagcaaca aacaagtaaa
    18001 agaagtatct ctaccagaag gggcaccggt tgatgtcgtt actgcattgg gatctgaact
    18061 gacaggtggc ggtttgaact tctcttctaa tggaggaggc ggagcccgag atggagtaac
    18121 tttccatggc gtaaatccaa aagatgaaga agtagagatc gattgggtca actattacca
    18181 agctgttgat acgtacttga aggattcttt cgaaaatcct gaacaatggc cgatttattt
    18241 atttgcatta ccagaaaatc agacgatgtt caaaaaaatt gctaaaaatc cctattattg
    18301 tgcagacgtt gcgatttctg cttctcctgc gcagctatcc tttagagata tcgaagaagg
    18361 ggcgaagaag ctgtcaagtg aactcgcagc tcaagaatca aaagaataca ataaactctt
    18421 agataagaaa ttcatcgatc agttagttga tatcgttcca gctgcgaaag aaggaaaagt
    18481 gtcacattta tttatcgcta cgtcaaactt agctgatggt ttcggtgaag atcctgaaac
    18541 agaatatgat cggcgtcaag tgttgaatgc attagcagat gatgtgatca ttaatggcgg
    18601 gaaagtgttt attttggatc aaaaagatgc gccagatgaa aagagtctga cagcgattct
    18661 tcgttattaa aagaggttgt gaaatcagtg ttttgacctg agtattagag aaacaaacgg
    18721 aaaaatagtt tcacatattt ttccgttttt tgagctaata ccgcagatgc aagcttttga
    18781 acaccgttta aggaagaggt tgtgacagaa gtggtcagct tcaagaaata agaagagatt
    18841 gacaaaaatt atttctcaaa tttttgtgaa atttcgactt atttccgaag aagctacttc
    18901 tggaacaccg tttaaaaaag aggttgtgac gtggtcacga cctctttttg acgattatga
    18961 tacaaaaaac atcatttatg aacaaaatgt gaataacggg cgttcttgag atatgatttt
    19021 aattgggaaa aacttatcga taaggaggtc tttatgaaaa aaagactgtt atggtgtttg
    19081 ctgtttctat ccctaagttt aacaagtcag ccagtttttg ctgaagaaac acagcggtct
    19141 cctattacag gcacacgtct gtacaaaaat gtttatgtac agacaacaga tcttgattct
    19201 gcgattcctg aaacagaaga acggtctttg cagccaagat ttggttactt ttggtactgt
    19261 ccgacctgtg attatgaaag ttgctggcat ttcttacatt ctacagctgc agcttttgct
    19321 tttactcata ctcagaaata tgatggatat catcctgtta tttatgaggt atagatatga
    19381 gcaaaaaaga aaaactaatc tcgttgatcg tattggtttg ccttgttctt ctggtaactg
    19441 tcataggcta tcagaaatac catgagaaca tcagacctac taaagaatct tccccagcct
    19501 cgaccgttga aacagaaccg ataagtagtc agaaagaata atttttttag tttaaagaag
    19561 aacatcagag caaaaaaaca ccaactgaac aatatgtcgt ttattttaaa aaaagttcgg
    19621 tttttcactt tatgtaatcg ctgagaaaac tttttttaat aaaaaaagtc gaaaaactct
    19681 agcttttttg taaacggtat gatatactat aggagtagtt tttgttcggt aatctgtggt
    19741 agcttgtttc aaataaacat cttccagata tacaccattc aagtaattgc aaaatatgtg
    19801 ttgatacaca aggaggattt ttattatgga aacaggtaca gtaaaatggt ttaactcaga
    19861 aaaaggattt ggattcatca ctgcagaaaa cggaaacgat gtattcgttc atttctcagc
    19921 tatccaaggc gacggattca aaactttaga agaaggccaa gcagtaactt tcgatgttga
    19981 agaaggccaa cgcggacctc aagcaacaaa cgttactaaa gcttaattaa cctatactaa
    20041 aaaataaacg cccagacatt tgcgtgtctg ggcgtttttg tctattttct ttaaaaagcg
    20101 atgagagtat ttttctgcac tttgtagtat aatttcaaaa gaacattaga ggcaggagaa
    20161 gaggaagata ttttgaaacc aagaacttat gcagtcgttg atatcgaaac gactgggacg
    20221 aatccaaaag aagatcggat catccaattt ggctgcgtca tgattgaatc aggtcggatc
    20281 acttcacgtt tttcgatcga tattcatcca gggcggaaga tttcaaaaca aatccagcat
    20341 ttaacaggga tcacgaatca acgcgtccaa aaagcgcctt atttcgaaga tgtcgcccaa
    20401 acgatttaca atcttttggc agacaccata tttgtcgcgc ataatattta ttttgattat
    20461 aattttttga accatgaact catgcgatgc ggacttccta gtttgaagat accaggcatc
    20521 gatacagtag aacttgcaca aattttcttg ccgacggaac caagctttcg cttagctgat
    20581 ttgtcagaaa gccttggatt tattcatgag aatcctcatc aggcagatag tgatgccgaa
    20641 gtgacgggtc aacttctttt gctgatcgaa gaacggatga gaaaattgcc aatcatcacg
    20701 ttagagcaga ttgcgcgatt aagtagacac acaggtatgg atacgagtcg tttcatttat
    20761 catgtgattg aagaaatgaa agaaaaacca gagccacttg atccctcatt agaaatcgtc
    20821 gatgggctag ctttgcagaa aaaagaagtg gaactattca tatacgtcca ttatggcgag
    20881 cggacatatc caagaaaaaa acaagcaaaa gaaaaaatgt ttggtaaaac actgatgtat
    20941 cgaaaagaac agaacagact gatgaatgcg gtctacgacc atttcacaaa agatgaatca
    21001 aaagatttga tgatcgaagc agctacagga atgggtaaaa caatcggtta tctgctgcca
    21061 ttgagttatt tggctacacc tgaaaaaccc gctgtcatca gtaccgtttc gctagttttg
    21121 cagcagcaga tcatagaaaa agacattcca ttgctgaatc aattgctaga tcagcccata
    21181 caacctgtaa ttatcaaaag ctatcgccac tatattgatt tacagcgttt taaaggcaca
    21241 cttgtggaac cgccagaaca aaaacaatat gctttgtatc aaatggcggt cttagtttgg
    21301 ctgacgcaaa cgaagaccgg tgatttggat gaattacatt taacaaattt gaaccattca
    21361 ttctttgcag atatcgcgca cagaggcact ggatttttag caagaaatca gtcattttac
    21421 gaacaagact ttgttcgttt cttgcaaaag aaaatcaggc aaagtaattt tttgattgtc
    21481 aatcatgcgt ttttaataca agaaacacag cgaaaagaac cattgcttcc agctagtcca
    21541 tttttgttga tcgatgaagt acatcatttg ccggaaatcg ctgaaaaagt aaatgacaaa
    21601 cgattaagta ttaactattt ccataaacaa atccagcaat tagaagaacc aaatcagctg
    21661 tttgaccgaa tacaaagcta tttaccgaaa gaacatgaag ccttacgctg gttatcgatt
    21721 tatcgagaag aactttctgc gctatgcgaa gcacagattt gtatggcaga aggattaatg
    21781 agtttagtga gcggcaagca acctaacgga cagtatccag aagaagtcat gacgacaaaa
    21841 gagatgttgg atcagctgcc actggaatct gggacagcca ttgatcacct gcagcttttt
    21901 tatcaggaga tccagcagct acagtcacaa atgcgtctga cttttgagat tatccaagaa
    21961 agctggacaa accagatgcg gatcgattta gcaagtttat atagcttatt tgatcaatta
    22021 gacaagcagt acttgctgat ggatcaatgg ctggaagact ggcgagatca tttgatccat
    22081 tggttcgttc ctgggaacac agcgcagcag atgatttttc atctccatga ttttcaagcg
    22141 gctatgttgc cgtcgactgt ttggtatcct cgatatgatc gtattcttta tattggagga
    22201 acgctgaaga tcggccctaa tcgacactat ctctctaagc ggcttggcat tgaagcagca
    22261 cctcttaaag tgattgcctc tccttatgat tatgagaaac aggcaaggat ctatgtgcca
    22321 aaagaggcac ctgccgtcca tcaagtcagc aatcaagcat atatcgatta tttagagaca
    22381 gcacttacag gtctgatcga acaagaagat cggccaatac ttgtgttatt cacttctcat
    22441 gatgttttgc agaaggtcta tcagaaaatg catctgtcta tgctagaaaa aggacgagaa
    22501 atactagctc aagggtttgg cggaagccgg gagaagcttt tgaaacgctt cattttgtca
    22561 gaaaaaagcg tattgtttgg cgctgacagt ttttgggaag gtgtagattt gcctggagat
    22621 gctttgcaga tcgttgtagt cacacgttta cccttcgata atccgcttcg cccgatggtc
    22681 aaagctagga acgcttattt agaatcagag gggatcaatc ctttttacca ggaatccgta
    22741 cctaaagcag ctttgaagtt acgccaagca ttaggacgct tgataaggac ggagacagat
    22801 cgtggcgtat tgcttatttt ggatcgaaga ctagttactg caaaatatgg tgcacggatc
    22861 atccatgcat taccgaaaaa agtaccgatc aaagagctag ctatggagga aatctatcag
    22921 gatatccatg aattcttaaa gaaaaatgaa gaaggaagtt agaggagctg tcttttttca
    22981 taattaaaat ctgctataat gaaaaggaat gaagataaag gagcgacgac ccttgaagaa
    23041 aaaacagaca aaggaaaaaa cgattaatcg cgtgttactt ggcttgatcg tatttttatt
    23101 agcggtcatc gtgatcagct cgatttttta cttgcgttcc agtcgtccgg cggtgcaggc
    23161 gaagaaagaa gcagtagaaa tcgcaaagaa atatgcaaat cttgaggaag ccgatcattt
    23221 ttattggttt actcgaaaga agacatactt ttcactgaca ggtacaaatg ataaaggaaa
    23281 agaaatcgcc gttatcattc ctaaatctgg tgaaaaagta aaagtattgg atcaaacaaa
    23341 gggactgact gaaaatgaag caaaacagac aattgctgcg gcccatcctg aaatccaaat
    23401 cgaaaaagct gctttaggga tgtatgatga tcagcctgtg tgggaagtag taggaaaaga
    23461 aacaaatggg aaactgaatt attacctgat tggctttgaa tctggagatg aagtcaagac
    23521 aatcaaggga atttaaaagg taaaggaatg aatgggatgg agttatcaaa aagaacaaaa
    23581 cgattagaac cgtctgtcac tttggcagct gcggcaaagg ccaaggcatt aaaagccaaa
    23641 ggcagagatg tgctgagttt gactgtggga gaaccggatt ttgcgacacc agaaaacatc
    23701 caagaagcag caatcgaagc aatccgcaat gggaaagcta gctattatac accaacagca
    23761 ggaatcccag agcttcgaca ggcaatcgtt gattatctga agaaaaacta tggacttgtc
    23821 tatgaacctt cacaaacagt cgtaacagac ggagcgaaat ttgctttgta tacccttttc
    23881 caaacgatcc ttgacccgca agatgaagtc atcattcctg tgccttactg ggtcagttac
    23941 ggtgagcaag taaaactggc agaaggactt cctgtctttt tgaaaggaga agaatccaat
    24001 agctggaaag ttactgttga gcagctagaa caagcacgtt cagaaaaaac aaaagccgta
    24061 atcatcaatt ctccatcgaa tccgactgga atgatttata gcaagaatga actgcaggca
    24121 atcggtgaat gggcagtaaa aaatgatatt ttgatagttg ctgatgatat ttatggccgt
    24181 ttagtttata atggcaatga gtttactcca attgcaacga tttcagaagc aatcaaaaat
    24241 cagaccatca tcatcaacgg cgtgtctaaa acatatgcga tgacaggctg gcgcattggt
    24301 tatgcggtgg gaaatcctgt gatcatcaat ggaatgatcg ctgttgcttc ccaatcaact
    24361 agcaatccga cagcagtcag tcaatatgca gctgttgaag cattgaaagg cgagcaagat
    24421 acagtggaag aaatgcggaa cgcctttgaa gaacgcctga atacgttata tccgcttgtg
    24481 gcagaactgc cgggtgtttc tcttgaaaag ccacaaggag ctttttatct ttttcctaat
    24541 gtaaaagaaa cgctacgcat gtgtaaatat gaaaatgtca cacaatgggt agaagatctt
    24601 ttagaagaaa caggggtagc gcttgtgact ggcgaaggct ttggggcacc agaaaatgtc
    24661 cgcatgagtt atgccactga tcgcgctacg ttagaagaag cggtacgcag aatcaagcaa
    24721 tttattgaga gtaaaagtca aaattaatcc tgaagaaatt caaggaatat aaaaggagag
    24781 tcatacgtgg aacaaattca aatcatcgat tcgaaaaacc atgtgggaga aacagtaaca
    24841 atcggcgctt gggtcgcaaa taagcgatca agcggaaaaa tcgcattttt acaattaaga
    24901 gacggaacag cctttttcca aggtgtagtt gtaaaaagcg aagtgccgga agaagttttc
    24961 caaacagcta aaaatctgca ccaagaaaca tccgtgttag taactggaga aatccgtgaa
    25021 gacagccgtt caaaatttgg ttatgaaatc ggaattacaa atgtggaagt ggtaagcgag
    25081 agccacgatt atccgatcac accaaaagaa cacggaacag acttcttgat ggatcatcgt
    25141 catttatggc tacgttcttc aagacaacat gcgattatgc agatcagaaa tgaaatcatt
    25201 cgtgcaacgt atgaattctt taatcaacat cattttgtca aaatcgatcc gccgatcttg
    25261 actggttccg caccagaagg cactacggat ctattcgaaa caaattactt tgatcaaaaa
    25321 gcttatttat ctcaaagtgg ccaattatac atggaagctg ctgctatggc tttcggaaaa
    25381 gtcttttcat tcggtccgac tttccgtgct gaaaaatcaa aaacacgacg tcatttgatt
    25441 gaattctgga tgatcgaacc agaaatggct ttcactcatc aagaagaaag tttagaagta
    25501 caggaacaat atgtagcatt cttagtacaa aatgttttag atcattgcga atacgcatta
    25561 gatgtactag gacgagacaa agaagtctta aaaacttata caaaattgcc attccctcgt
    25621 attacttatg atgatgcgat cgaattactg aagaaaaatg gatttgatga catcgaatgg
    25681 ggcgaagact ttggttcacc tcatgaaacg ttcattgcga attcattcga acagcctgtc
    25741 tttatcctaa actatccaaa agctatcaag ccattctaca tgaaaccaca tccaacaaga
    25801 gaagacgtgg taatttgtgc agatatgatc gcaccagaag gttacggtga aattattggc
    25861 ggaagcgaac gggcaacgga ctatgatttc ttgctagaac aaatccgtaa tcacggtttg
    25921 gatgaaaaag aatattcatg gtatcttgat ctacgtaaat acggttctgt tccgcactct
    25981 ggttttggtc taggccttga acgaacagtc acttggattg ctggtatcga ccatgtgcgt
    26041 gaagctattc cattcccacg tttgttgaac cgaatttatc cataagaggt gatgaaaaag
    26101 ctgttctgca tcaagtaatt agaacagcca agcaaaaaca gctgttcatg tttttgaatt
    26161 ggcgggactt attaccgcag atgtaagctt ttgaataccg tttagtggag aggttgtgaa
    26221 aaagctgttt agtttcgagt attaagaaaa attttgtgga aatggctttc cacatttttg
    26281 cataaatttg gcttaatacc gagaaactgg cttttgaaca ccgtttaagg aagaggttgt
    26341 gaaatcagtg ccttgacctg agtattagag aaacaaaacg gaaaaatagc ttctcatatt
    26401 ttaccgtttt tttagctaat atcgagggtc agctgattga acaccgttta tatcaagagg
    26461 ttgtgcgcct gccgttcagc tccgagtcac aaggagtaat ttcaaaaaac agtttttcat
    26521 gtttgatgac tattgtgact tgtgaccgag gagttggccg gagagcaccg tttattctaa
    26581 aaaagaactg cgaactctcg cagttctttt tttaaaatac gggataaagg aagtttccag
    26641 cagacaagag aagaaagcaa aagaaattgc tatttgattc acaaggttct ataacgaaat
    26701 cgcaatcaat caaacattta caaagaaggt aattcaaatg gaaatctgat ttgatgatgc
    26761 ttctgctgtt gaaataaagc agcatctagc acctgaaaac aaactaatag taacattcga
    26821 agatggagta ggtccgtatt ttcagcatgc aataatccat atgtaagttc aattcaccct
    26881 tgaaatcatc ggaccagata aagttatgac caatctatcc caagcaatct agaagagata
    26941 ttagtaaagg gacatttact agaagtctta gatgagccta taaatatatg ttctgatggc
    27001 gtcatcagtg gtctgattga cggaagctta ggtgtgatcg atctctctgg accaaacaac
    27061 gaaggaatca aggggaatcc ggcacgttaa gaaaaaaata gcataaaaaa ctagacactt
    27121 gatgagcgtt cattcaattg tttgaaggga ctaggctggt gtcatttttt atacatatat
    27181 tttctctttc gtcttgatag ggcttatggg ataatataag gaagaaaatc atcgttttat
    27241 tcaaaaagga gtttttgaca aatgaaaaaa cgtatattat ttatcggtga tagcattaca
    27301 gacgcaggaa gagaccgagc agaagcaact gatctaggta aaggttatcc acttttgatt
    27361 gccggaaaac taatctccca atatccaacg atggaatttg aatgcatgaa ccgtgggata
    27421 ggaggagaca aaattgcaga tctgcaagta cgctggcaag aagattgttt agctttacaa
    27481 cctgactatg tatcgatttt gatcggaatc aatgatacct ggcataatac tagcgatcag
    27541 gctatattcg gaactgagaa atctgctcag caatttgaat ccgtttatcg aaaattacta
    27601 aatgaacttc aaacagtggg aatctgcaac atcatcttga tggagccgtt tgtacttcct
    27661 ataaccgacg accgtcatgg ctggcgtaaa gacttagacc ctaaaatcca aattgttcgt
    27721 aaacttgcta aggaatatgg cgcattgctg gttcctttag atggtatatt caatgcttta
    27781 gggattgttt atggtttttc gaaatataca agagaagatg gggtccaccc aacgattggc
    27841 ggacatgaga tcattgcaca aagctggata gatgcaattg ccgcagtcga tttttttagt
    27901 ggagataaaa atgagcgaaa gaaaagtgag aaatgatgga aatacggtgg gcagtaaaag
    27961 aagatctacc tcaaatcaat cagctgatta ctgatcatcc agaatcattc gtaaaagcaa
    28021 aaacggcagc tgacctaaat gataagaata aactattgct cgttgctgta acaaaagaaa
    28081 aacggattat cggatttgcg caagcggacg tgcaacagtc agaagaacga ttggatcagc
    28141 gaccatcagg aaaactaatc caacacacat tagtaacaaa agaaaatacg gaagaaatcc
    28201 aaaaaaaatt tgcagaatat ttgatactgg aagctaaaaa aagaagaata aaatcatttt
    28261 ttgcccagtt ttaatcaaaa acagtaatag aaggagaaat acaatgagtt gtttaggaaa
    28321 tgtcatctgg tttatattcg gcggactaat cggtggtctt agctggttag cagcggggat
    28381 actttggtgt atcacgatca taggtatccc aatcggtatg caatgtttta agatcgcctc
    28441 cctttctttt gcgccttttg gcaaagaagt aatttatcac cagtctggca tgtcattgat
    28501 tgttaatatc atctggctga tcgtttcagg cctaccaatt gctctcgcac atgtcgtcag
    28561 cggcatcctt ctatgtatca cgatcatagg gatacctttt gccaagcaat ctttcaaact
    28621 tgctcgattg gcgttgatgc cattcggtgc agaaattgta taaaagagaa taataaaggt
    28681 tagataaagc atagagtcag gtgggataca tgtatttcac ctgactctat gcgttctcct
    28741 gtataagttc ttccatgtga cttttacgtc ttttatttat atggggttac tgtttatgga
    28801 aagttgaaat aattttcatt aaagaactct ttctatattt ttgaaagcgt agtggagctt
    28861 cgaatatctg ggaaggacat tttgcaggga ttgaaaaaag atattgtcct atcgaaaaat
    28921 taatgaattt agacaaattg ccagcaacag gcttcaaact tagttgctat cctgtcaaga
    28981 tcaagaaggc aagtgcgggt tggataagag cagtggcaat gatcgaaaag aaaaagaaag
    29041 aataagccaa aacatctgta tggaagaaat tttccgatgc agatgttttt cattttttct
    29101 ttcggatacc ttgacatatg aataaccgtt catttataat tagttatgaa gagatattca
    29161 tatgttgagg cgaggaggtc attaaaatga aaagttacag attagaaggt ttagattgtg
    29221 cgaactgtgc aatgaaaatc gaaaaaggag ttcagaagat caatggagtc aaggaagcaa
    29281 cggtaaactt cacttctgga aaactaacga ttgacgcaga ggaagatcat ttggctacga
    29341 ttgaacaaga aataaaaaaa gtagtgaagg aattagaacc agatgtcaaa gtaacagaaa
    29401 tagataagga aaaagtatct gaacatggaa atgaaaaaga gagaaatacg ctgttccgga
    29461 ttttattttc ccttgcaggt atcgctttat tgctattatt tgattttaat gaaccaattc
    29521 gcttgatagg gtatttattg atttatctgc tgataggata tgatgtagtg aagaaggcag
    29581 tgatgaatat cgtcaaagga aaaatctttg atgagaattt tcttatgtct gttgctacga
    29641 ttggcgctat gttgatcggt gaatatccag aagccgtagc agtgatgcta ttttatcaaa
    29701 tcggcgagta tttccaagga ttggcagtca gtcattcaag aaaatcgatt cgcgaattga
    29761 tggcgatccg tccagaaaca gctcacgtgc agacgacgga aggattgatg accgtgaacc
    29821 ctgaagacgt gcagatcggt cagtttgtac ttgtgaagcc tggtgaacgt gtaccgttag
    29881 atggaacgat catcgaagga gaatcattag tcgatacgtc agcacttaca ggagaatcag
    29941 tccctaaatc tgtatatgtt ggtgaaacag tattaagtgg atttatcaat aaaaataaac
    30001 catttttagt tcaagtagaa aaatcttatg aaaattctac tatcagcaaa ttattagaat
    30061 tagtagaaaa tgccagcagt aaaaaagcac cagctgaaaa tttcattact aaattcgcgc
    30121 gctattacac accagtcgtt gtaggattgg ccgttttatt agctgtttta cctccacttg
    30181 ttgtttctgg agcggcgttt tctgaatgga tctatcgagc attgacattt ttagtgatct
    30241 catgtccatg tgcattagtg atctccgtac cccttagttt ctttggcggt atcggaggag
    30301 caagtaaaat cggtgtatta gtaaaaggtt caaattactt ggagttgctt gcacaaactg
    30361 agacagtggt attcgataaa accggcacat taacgaaagg tgacttttcg atccagacga
    30421 tcgatacaaa aatcgatcca aagatattca tgcaatatgt tgcgagtgca gaacagtttt
    30481 ctacacatcc gattgctcaa tctgttttag aaggctacgc aggtccgctt ttaccaacag
    30541 ctaatatcca agaatttgca ggtgaaggga tcttagctga agttgatggc aaacaagtat
    30601 tagcaggtaa tcacaaattg atggaaagat ttgaaatctc ttttccttca agtcaagaaa
    30661 tcggtacatt gctttatcta gcgatcgatc aaagttacag tggttatctt gttattgcag
    30721 ataccttgaa agaagatgcc gtagacgcat tggttcaatt aaaaaaggct ggtgtgaaga
    30781 acacagtcat gttgacgggc gattcgaaaa aaattgctga tcatatcggg aagcaagtag
    30841 gcgtggacaa gatatatagc gaattgcttc cagaagataa agtgcagcgg ttagaggaaa
    30901 tcttacaaag aaacaataaa aagaccgcat ttgtcggaga tggaatcaac gatgcaccgg
    30961 tgctagcaag agcagatgta ggaatcgcaa tgggtggatt gggaagcgat gctgcaattg
    31021 aagcagcaga tgtagtcatc atgaatgatc aaccatcgaa gattgcagaa gcaatccatt
    31081 tagcaaagaa aacattgaaa atcgtcaaac agaacattgt ctttgctatt gggataaaaa
    31141 ttttggtact tgccttaggt gcttttggtt ttgcatcaat gggagatgca gtatttgcag
    31201 atgttggggt gacagtatta gctgttttga atgccatgcg aagcttgcat gtaaaaaata
    31261 aacgttgatc aatgaagagg acgaatgaac gtcctctttt ttttatatcc ttttcttaat
    31321 gaaaaatgta aagaaaatag ata
//
LOCUS       DACCXO010000021        30950 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.022, whole genome shotgun sequence.
ACCESSION   DACCXO010000021 DACCXO010000000
VERSION     DACCXO010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.022"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            56..1507
                     /locus_tag="IVY70_000924"
     CDS             56..1507
                     /locus_tag="IVY70_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP7958071.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1856..2488
                     /locus_tag="IVY70_000925"
     CDS             1856..2488
                     /locus_tag="IVY70_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP7958072.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3076..4515
                     /locus_tag="IVY70_000926"
     CDS             3076..4515
                     /locus_tag="IVY70_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7958073.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4810..5028
                     /locus_tag="IVY70_000927"
                     /pseudo
     CDS             4810..5028
                     /locus_tag="IVY70_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5277..5492
                     /locus_tag="IVY70_000928"
     CDS             5277..5492
                     /locus_tag="IVY70_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958074.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5709..5965)
                     /locus_tag="IVY70_000929"
                     /pseudo
     CDS             complement(5709..5965)
                     /locus_tag="IVY70_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(6152..7063)
                     /locus_tag="IVY70_000930"
     CDS             complement(6152..7063)
                     /locus_tag="IVY70_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7958075.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7082..8086)
                     /locus_tag="IVY70_000931"
     CDS             complement(7082..8086)
                     /locus_tag="IVY70_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP7958076.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8083..10206)
                     /locus_tag="IVY70_000932"
     CDS             complement(8083..10206)
                     /locus_tag="IVY70_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7958077.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10211..12658)
                     /locus_tag="IVY70_000933"
     CDS             complement(10211..12658)
                     /locus_tag="IVY70_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7958078.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12645..13034)
                     /locus_tag="IVY70_000934"
     CDS             complement(12645..13034)
                     /locus_tag="IVY70_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7958079.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13094..13597)
                     /locus_tag="IVY70_000935"
     CDS             complement(13094..13597)
                     /locus_tag="IVY70_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP7958080.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13610..13834)
                     /locus_tag="IVY70_000936"
     CDS             complement(13610..13834)
                     /locus_tag="IVY70_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958081.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(13938..15848)
                     /gene="ltrA"
                     /locus_tag="IVY70_000937"
     CDS             complement(13938..15848)
                     /gene="ltrA"
                     /locus_tag="IVY70_000937"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP7958082.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(16594..16713)
                     /locus_tag="IVY70_000938"
     CDS             complement(16594..16713)
                     /locus_tag="IVY70_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP7958083.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
     gene            complement(16728..17912)
                     /locus_tag="IVY70_000939"
     CDS             complement(16728..17912)
                     /locus_tag="IVY70_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7958084.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(18171..18461)
                     /locus_tag="IVY70_000940"
     CDS             complement(18171..18461)
                     /locus_tag="IVY70_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958085.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(18451..19593)
                     /gene="dcm"
                     /locus_tag="IVY70_000941"
                     /pseudo
     CDS             complement(18451..19593)
                     /gene="dcm"
                     /locus_tag="IVY70_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(19826..20500)
                     /locus_tag="IVY70_000942"
     CDS             complement(19826..20500)
                     /locus_tag="IVY70_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7958086.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(20578..21918)
                     /locus_tag="IVY70_000943"
     CDS             complement(20578..21918)
                     /locus_tag="IVY70_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP7958087.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(22001..22336)
                     /locus_tag="IVY70_000944"
     CDS             complement(22001..22336)
                     /locus_tag="IVY70_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP7958088.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(22346..22720)
                     /locus_tag="IVY70_000945"
     CDS             complement(22346..22720)
                     /locus_tag="IVY70_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7958089.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(22733..23047)
                     /locus_tag="IVY70_000946"
     CDS             complement(22733..23047)
                     /locus_tag="IVY70_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7958090.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(23160..26387)
                     /gene="fss3"
                     /locus_tag="IVY70_000947"
     CDS             complement(23160..26387)
                     /gene="fss3"
                     /locus_tag="IVY70_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP7958091.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            27175..27498
                     /locus_tag="IVY70_000948"
     CDS             27175..27498
                     /locus_tag="IVY70_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7958092.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            27662..28579
                     /locus_tag="IVY70_000949"
     CDS             27662..28579
                     /locus_tag="IVY70_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7958093.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            28572..29354
                     /locus_tag="IVY70_000950"
     CDS             28572..29354
                     /locus_tag="IVY70_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7958094.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            29355..30035
                     /locus_tag="IVY70_000951"
     CDS             29355..30035
                     /locus_tag="IVY70_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7958095.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            30028..30924
                     /locus_tag="IVY70_000952"
     CDS             30028..30924
                     /locus_tag="IVY70_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7958096.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
BASE COUNT         9085 a         6301 c         5583 g         9981 t
ORIGIN      
        1 tatcataatt tttaatagaa aacactatta aagagaaaaa agagaggtaa atcaaatgaa
       61 aaaatcttta aaacttgcat cagcagttat attaatgtcc caactcctta tagcaacacc
      121 tttccatgtg ttggcaagcg aaaattcaac atacaaaatt gcaaaagaaa gaaccgagac
      181 gactgaaatg agtaattccg atgtaacagc agagagtagt gaacttttga caacttcatc
      241 aagtgaaagt agtacgactt caaatgccga gtcttcaaca acttcatcaa gtgaaagtag
      301 tacgacttca aatgccgagt cttcaacaac ttcatcaagt gaaagtagta cgacttcaaa
      361 tgccgagtct tcaacaactt cgtcaagtga aagtagtacg atttcaagta ccgaatcttc
      421 aacaacttca agtagtacaa gtgaaataac ttcttcgaaa gtgacaaaac caaaccatat
      481 agaatcttca aaaaaggaag cgccaaaaac ggaaggacaa acaaaaactt tttcgacacc
      541 acagcaaata gaatcaacag aatctttcca atcacaacca atactatctg gtaatgaaga
      601 aattcatttt gacaaaaatg aatctgtaga aaattttatt atgaaaattg gtgagtctgc
      661 gagaaaaatt ggtcagaaat atgatttata tgcttctgtt atgattgcac aagcgatctt
      721 agaatctgcc agtggtcaaa gccagttagc tcaggcaccg aattacaatt tatttgggat
      781 aaaaggaact tataatggaa actttgtcat tatggtaaca aacgaagatt tgggtaatgg
      841 aacgctttat acgacccaat caaaatttag agtgtatgaa aactatgagg aaagctttga
      901 agattatgcc aaattattaa ctaagggaat tagtggaaat aaagattttt atgcaggagc
      961 attaaaagct aatagtaaaa catatcgaga agcaactaaa ttccttacag gacggtatgc
     1021 aacagataca caatactatt tgaagctaaa tgaattaatt aaaacctacg atttaaccaa
     1081 ctatgataaa gaagttaaag gtccacaaat gagtaagact ggataccaag tgcctattca
     1141 taattattca ataaccagtt tgtttggaaa tcgtggaaat gaatttcacc gaggacttga
     1201 tatggcagct acacagggtg agccgattca tgcagttaaa gctggtaagg ttgttaaagc
     1261 agagtttcac tactcttggg gaaattacgt ggtgatacaa cacgaagatg gatcaaccgc
     1321 gttatatgct caccagcaac aatacctagt aaaaactgga gataatgttt tgcaaggaca
     1381 aataattggc tatgtaggaa gtactgggaa tagtactggc ccacaccttc atttggaaat
     1441 ttgcaaagat agtagtcttt ctcagagcat gttagttgat ccgaaaacct tattattcgg
     1501 agactaaaaa aggtaatcaa tctgacaaag taacaagaaa atggttggat tcttagacct
     1561 ttgatcagga tattgagact ggaattgata tctcgattac gatgggtttg acaaataaaa
     1621 caagtccatt atcgaatatc gagtgtatta ttgccgtctt tatgctcata ttttgagcag
     1681 gtttcactag atgaaaacaa tttatctatc ttgaggattt cgtgtacgta tcgttcagtc
     1741 ttgtattgta aattagtcat aaaatcagac taagaaaaat tggaaatatt tttttctgat
     1801 ttattaaaag ttaattgaaa ttatatggta cttctttctt gggagggtag gtgcagtgaa
     1861 ggaaagatat ggtcacctta ctgttgttga ggagattact cataatggat ataaaaaata
     1921 tcgttgccta tgtgactgtg ggaatgaaac gattgtatat cgatcaaact tactatcagg
     1981 tagaaccaaa agttgtggat gtggaaatta taaaaatagg cataaatata aaaatttgac
     2041 aaataaaact tttggtaagt tagttgcaat caagccaact aaactgcgaa gtgaaggaac
     2101 tgttgtttgg gaatgtcaat gtctgtgtgg gagaaaaaag ttagtatcgg caaagcgact
     2161 tctaaaaggt gatgtaaaaa gctgtggatg tttgaaatca aaaaagcatc acattgaaaa
     2221 taaaaaattc ggattactta ctgcaaagta tcctttggac aataactgtt catggaaaac
     2281 aaaatgggtt tgtcattgtg agtgtggcag tatgtgtgag ataagttata gtaacttatt
     2341 ttttggacat actcaaagtt gtggatgttt aaaaaggcaa gaaacaagga catgtgttga
     2401 agggacagta atagagtgtc taacgtcaaa attaccgaaa aataatacaa gtggtgttaa
     2461 gggagtttat cgttctaaag agaaatagat tgcgtatata actttaaaaa aaaagaggta
     2521 ctatttagga tcgtatgatt ctattaagag tgcagctcaa gctagaaaag atgctgaaaa
     2581 taaattgttt caacctttga ttgacaaata tcgaggtaat cattaattat tgtggataat
     2641 aattaacagt gatatataaa catctataat atattattca tactattgat atttagtata
     2701 atcctatgga attgaatatc atagcaatgt atttgaaaag caaattggaa aacaaatagg
     2761 tgcaacacca taaatatgaa aaaattggga aaaagtagaa tggaaatttg cttacgtctc
     2821 acgagatagc tcacactatt aatctgaaga acaatgaagt cgacttttag aaattgctag
     2881 aagaaagtaa agcgaattat cactagttac tatagagtaa gttcaaataa agcatctcca
     2941 acttacatgg ttacatgagg atatcaaatc gaaattactc aagcttccta tggctagagt
     3001 cgcgagaagt tgttgggcag tagacatagg ttatattgtt cttagctaca cgatcattag
     3061 aaaggaaatc ctattatgta tagttatcaa gaagatgcaa aagtccaaaa aaatcgctgg
     3121 tggattttga ttgctatatc tatgtttacc ttcatgtcca cattggatgg cagtattgtc
     3181 aatattgcac tacctaccat ctcagatgat atgggcatac caatgaatca agcggaatgg
     3241 gtagtgtccg tttatctgat gattgtctgt tcttgtttgt tgctgtttgg taaaattggc
     3301 gatagccgtg ggaaaatcaa agtatttcgg attggtacgc tgatctttgt cttgggatcg
     3361 ctgttgtgtg ggttcaatca ttcgctaggt attttattgt tcgcacgggt ggtacaagcc
     3421 attggtgcta gtatgactat ggctacaaat gccggaatca tcacggaagt ctttcccata
     3481 catgaacgtg gtcgggcatt gggagcaata ggtgcgtttg tttcattagg ctcgattgct
     3541 ggaccaggtc ttggcggctt gattttgtcc caattttctt gggcgtacat tttttggatc
     3601 aatgtaccgg tgggaatcgt aacgatgttg attgctgaaa aattcttacc aaaagatatc
     3661 accatgagcg gccatacaat cgatggaggg ggctttgctg ccattgcggc tacgattatc
     3721 agcttttttg gagcaatctt tttggggcaa gaaagtggct ttttccatcc agtccccctt
     3781 gtactgttcg taatttcagt gattgccttt gtcagctttt taaaagttga aaagaaagca
     3841 gcctctccat tgatttcttt cggtattttt aagaacaaga tttttaccat gagcttgatt
     3901 acagccttgt tgattttttc ttccaacttc tttgtcaatg tagtgattcc tttttatctg
     3961 caaaatgctc gcgggttgag tcctagctat gctgggctgt tgatgatggt ctttcctttg
     4021 atgatggtcg tcggggcgcc tttaagcggc tacctgaccg accgggttgg cccggagatt
     4081 ttggtattag taggcttggg cttattgtcg gtaacccagg tcatgtatat gtttttgtca
     4141 gtggactctc ctttgtggct atacattgtc gctactgcca tcatgggtgc cggcaatgcc
     4201 ttgttccagt cccccaataa tacgatggtt atgagctcgg tcactatcga aaacttggga
     4261 ctggcaggct cgatgaattc ctttgcccga aatttgggga tggtcagcgg gatttcgctt
     4321 tctacgacgg tcttatatca agcgatgtct gctaagatgg gccagcaagt aactaactat
     4381 atcgcaaatc ggccggatgt ctttttatat ggaatgaaga ttaccttttt aggcagcttt
     4441 gttttgtgtt ttgttgcctt tttcctgact atctggcgta tcaaaaaaca aggaggtatt
     4501 cagaaaaatg tctgaaaaaa ccactgttga agaatttttg gaggcctgcg tatgggtgag
     4561 tgggatttcc atggatgatg gaagatgaag tgaacttcta aaagccgaaa aactcaagta
     4621 ttatcttcaa aatactagtg ctcaatgaga tgcttgattt gatcacaaga agactgcgaa
     4681 agccgctggc aaccgcaagc taggtatccc accgatggac tacgttttag aggctactgg
     4741 ttatgaatac ggagccaacg ctctcatctg cataagaata catcacccag attatgtaaa
     4801 gattttcgat acttctgtca aaaaatcatt aagatagata aatgatttcc gtctagtcaa
     4861 attggttcag aatgtagaca cacagatggt aagaagtcac tcacaattcg agaatggact
     4921 tgtcctattt gccgtataca tcataatcga aacgtcaacg atagtatcaa tattttgacc
     4981 aagggctatg aatacaagca ttgccttaga aaacatcaag aaccttagga acaacgggga
     5041 tagcttggtt aataagagat accgctgttt aggcttgcaa gtatactctt ttatcaagaa
     5101 ttccgtggct ttagtcatga gaggcttaat actatgaaaa ttttctttag catttttgat
     5161 ttaggttcct tttctaatta taataagatg accactacag taacctactg aaatatataa
     5221 acagccactg tagtaccatt ttcaaagtag tctatgagag gagaatcttc attcttatga
     5281 acgctggaat tgcggatatg aatttaataa aaaaaaccct taatgatttt acttccaatt
     5341 caatatcaaa aggtactggt atcaatttaa gcacaataaa aaagctgaaa agtggtgaaa
     5401 gatcagtgga aaagctgaat cttttagatg ctataaaaat aacggaattt gctatgaaaa
     5461 atggaaaagc tgagattgaa atatggagat aattatcgtc tagtgaatta cttgccactt
     5521 agctgaattt aacgatcctt ggtgcttgaa gggggagatt cttgggaata gagcatactt
     5581 gcaagcctat gtctttacta acaacgatat ctcttattaa ccaagctatc cccgtagttc
     5641 taaggctttt acttttatct aagcaataat cgtattcaaa ggaaattttt gacccttaga
     5701 aagtctcatt ttatcagacg tgagcttagg cattgctgaa ttttgttaat tcggttttag
     5761 gttgtgcttt gagcataata tgaaagtatt ctttatcacg atttcattca tctaaggtaa
     5821 tgtgatgtga ttctgaaatt ctttcaaagg cagtttttgc ataagctgac attacatcat
     5881 caatgacatg tttacaatgt ttcactacta aaataagggg gtaaccaaga agaaaaactg
     5941 aatgattatt tgtatctaat tccatggtat atcaaccatt tcctatatag actgattatg
     6001 acatggatta aaataaaaag gcactatgtc ttaattgtcg gttttcgagt taccgaatgg
     6061 cattcatcac ccacctatag aggatgggcg acttctgcct gaaataagtt acactactta
     6121 taacttctaa taaaattaga ttgagtcatt attatttaac atcaacgatt ttccagtttc
     6181 catttttttg taatactaat ttgtactgaa agtagctagt ggtttttgat acattatcaa
     6241 ggtattttat ctttgtactc actagaaaat tttttccgtt ttgtagataa actggattct
     6301 ctaactccga aaactttaag tcaatattga ttggtcgcat cgcaccattt tcaacataat
     6361 actccaattc tttttcagaa gctgatggat aaagtttaaa aaatgtttct aaaaaatcgg
     6421 tgatttcttc cgttgttttg gcatcaattt ctgaatcgtt ttctacttgt tttgcttcat
     6481 attttgcctt gtctggcttt ccaatcattg ttggcaaact agttactaaa tgattcctgt
     6541 tttcatcttc aaagattgtc actcgatatg cagaagaaat tgattgcgtc ttttttccct
     6601 ctgtaatatg ttgctccact aaaaatgaaa cgacaaattc ttccgagtgt ttttcaacat
     6661 ctagaatttt tacagattga acttcggaac tcgtgggaat atctgctcga accatatctt
     6721 ggattaaagc gagaccgttt tctgtaaggt actgttcgag attactcatt ctcttttcaa
     6781 ttgcttcttt gttatttttc catgaaaaat attctttcgc aaaatcctct gtaaaacttt
     6841 caacgccaga agtattcact accttctctt ttataaccac tttttcatga gttgtatgtt
     6901 gatcaatggc ggtgaaattt ttataaacgc caaaagcaag actacaagac aataaaagcc
     6961 aaagggcaag caccattttt cgatgcttac ccacggatac aactcgttct tttttctttt
     7021 ttgggctctt ttccttttga ttccgttcaa tttttatctt cattcttttt cctcttttct
     7081 cttatttgta tcggccagct ccgataagat gctgccgcca atagttttcc gttagattcg
     7141 tccaccccac tggatctcct gcatgaaaca tgcgattctc ccctgcgtaa atgcccacat
     7201 gggtcacata agtaccggca tcataggttg aatgaaagaa aactaaatca cctggtttgg
     7261 catcttttag atcaatatga gtcatggcat catattgatc ctgagccacc cgaggaagtt
     7321 tcaggtcaac tttggcataa caccattggg tcaagccact acaatcaaac cccgtttctg
     7381 gacttgagcc accaaacaca taggccgttc cttcatactt aaatgcctca tcaaaaattt
     7441 cttggactat tttgtcatca aactgagttg tagttaggta ttgcttcact aataaacagt
     7501 aaaacatatt gccataatta taccgccacc caccattttc cttaatcgcg attgggttgg
     7561 ggtaatcgac tttttgacca cctgattttt cctttgaaaa agattcagct aaagcaaaac
     7621 tatgcttctt cccgtttttc ccgacatgat ctagataccc gccaccatag ttataggcct
     7681 gaataatact atcttgatca caccctagtt ctttggaacg cttaaccaaa gacgcaaaat
     7741 agagacagcc ttgtttgatg gatgcctcgg tacttaatga attaggtggt aatccggctg
     7801 actcagaact ttgcataacg tcttccattg tgccaccaga ttccacttga ataatcgcca
     7861 aaagaattgg aatatactct gttatatccg attctttggc atacttttcc accattgcac
     7921 ggtgtttcaa tacttcttct gagacactag ttccgccagg agaagtgcca gaactaccat
     7981 ttccctcact gtcttcacca aacaacaaac ccacacacat caaaataccc ataaaagaga
     8041 tggtcagaaa agttaatgaa ataacaccca ctttcttcaa gttcattttt tctcacctga
     8101 ccttgatttt ggacgatggt tcattctttt ttgtactttt tgattggtct gctttggcag
     8161 agtggacctc tgtttcactt gttggaaaga atttttctta cttgctgatt ggcttttccc
     8221 ttttgaaatg atttcttgac tagttagttt tcgtttgatt tcgggattca gatttgctct
     8281 cacaatccgc tttggtgctt cttttttcag catcatatct ttctgaggtt gagctgcttt
     8341 atctattgga atattcgccc ttctttttcc ctgacgattt gttgcttcac tcagtatctt
     8401 gcgtttttcg tccattttcg catttcttcg ttgtcgttgt ttttctcgca aatccactcg
     8461 atccgctttt tcttggaaca gtccgcgctt gaattcttct ggtgcttttt tcgacttttc
     8521 gattccttta tggagattgt atttcaaatt cgttggcgta ttccttacct gatcctttac
     8581 ttgtttagtc ttatctttca ccaaagcttg ggtatccaaa actttgccga tttttctccc
     8641 agccaaattc attcgggatt gtttcttatt atttgaaact ggattctcct tagttaagtc
     8701 agatagtatt tcgtgatcgt tcggtaatcg tgaattcttt cgtagagaag aaccggatgg
     8761 cttaaatttg gattttgatt gttcctttgc caacttattt ccgactgctg caccaccacc
     8821 aaaagccaaa gctcgtccca catttcgatt gagttgccgc aattttcgat tcattaacat
     8881 ttgcggtttt cgcatgacgc gtcttcccat tgactggcta tcattactct ggaggttgaa
     8941 catactcata atgtcgccca atttgaaata gataccggca aaggtcacaa tttgcagaaa
     9001 agcaaccatg aaaaatggat aggttgccga aatattgaaa aacatcgtgg agatgctaaa
     9061 tgcagtcgta atcaccaaag taacacccgc tcgcatcata atcgtattga ataaacgaat
     9121 aatcgccttc tttcctaaac tttcataagt cggcaacatg gacaataaga aactaattgg
     9181 cagaaacatc gcaaagataa tgaagagaat ttgcgagaac aacataatcc ctgttagaag
     9241 aaaaacaaag aaggaaatgc caatattgaa caataccaaa aagaccacgg tccctaaacg
     9301 gttcatggtc ttggtaatac tgagattcgc attgtcattg tcttcaatct ctgccttgac
     9361 cgcttcttcc cgatctttgc ctttgttttc atctggactc acagatagaa tcttgttgac
     9421 ccggtcttca ccaatttctt ctatgttgga atcatcaaat tgcagtagta accaaggttg
     9481 ttggacttga atcgaaaaca aactatcccg aatcaaatcc acactatctt ttccttggct
     9541 ttctgaattg ggtacaacga tctcggttcc aagggtaagg gccgcttcac taacatctga
     9601 gctaaagtca ttgatttttg tgatataggt aggcgcataa gcaatgaacg aacccgatag
     9661 tagaaaaatc atcacaaaat taatcacggc tcgaactgct ttcgtggttt ctcgttttaa
     9721 taatccggta tacgccacat acacccccag agctaaaatc atcaataata gaaacccaaa
     9781 ataaaatccg gaggtctgga gtccattttc agtaatacca gccaatgttt ggatattctt
     9841 tccaatactt tcagccgtat ccgaaatgaa atctaatttg taagcctctt gaaccacata
     9901 acccgtggcg ttcgacaaat acaaactcac cgtccaaatg aaattagtaa tggcataaag
     9961 accatacatc acgcttttcc ctaagccatc accccaattc caaggcaacc aatcccaaga
    10021 gctatccaca aagaaatcta gttgatagtt gttcagtgaa tatttggaat atagatttcc
    10081 tgcttcaact gtatcatcaa ctagccctgt cgcctcggct accgtaccaa ctaaactcaa
    10141 tagaactatc agagtgagta tgctaataaa aacgattccc acaatccgaa aaagtttctt
    10201 tttcatcccc tcactccatt ctctcttgaa caggtggacg ggtatcaaag gcatggaaga
    10261 gttcttcaaa gactggatgt acctgaacca cgcctacccg accatacaaa tcctgcatca
    10321 agcattggcc attttccaaa tctcgtaggc gtttttgatt gctttcatct tctcgatcca
    10381 agccaaagaa ttccaaggtt ttcttgattt catgaatatc ggtactgcga aacgcaaact
    10441 tcaacccgat attattcttc aagcgttcat ccagtaaatc atcactattt tgcgtgacaa
    10501 agtaaacccc ggcattcata gcccgcccag cacgaacgag cttattcgat aatgttttcc
    10561 cttgcgctac ttgaagaaaa ctccacgcct catctaagtc tacaattttg aagatttctc
    10621 gattactatg gataaagtcc aaggcaaaag tggaaatgac gattagcatg gaaacactaa
    10681 gcaattccat ggtggtatat tcttcaaaag tggtttctgc atctggtaaa accaaatccg
    10741 ctacttgaat gatatttaac tgcctatcta aactaatcga ctgggtaatc gaaccatctg
    10801 agaaaagcaa atgggcaaaa tcataatcgg taaaggactc gatgtgatcc gcgatatttt
    10861 ccgcaactgg tgagccttct tttcgaagct cattaattac ttgtaataat ccccgttgat
    10921 tgctttgggt aaccgtccga atggccttac gcaacactgg aaacttctcc ccatctcgac
    10981 tagaaattcc tgttaaaaag gtcagaatat caattgctag actttcagaa tccttctttt
    11041 tctccataat cacataagga tcaagtaatc cttgattttt tggcttacta gttaagttga
    11101 gaatattgat ttcatccgca atttctggca aggtttcttt ccagccaccc cgttcccctt
    11161 ttggatcgac aatcacagct tgtccaccaa aaaggacggc gtaataaact aagagattgt
    11221 tactaaagga ctttccgcca ccgagtgaac cgaggaatgc cgctgctagc gcattggtaa
    11281 ctgaatcctt gaccccttgt gaggccaatg ccggtttcag gtaaacattg cgccccgtat
    11341 ctacgttata accaaagtaa atgccttcca actcccctaa catctgagtg gcgccaaaac
    11401 caagtccggc gagaaaatca gacgtaacat actggatata atcattcatg tatcgcttac
    11461 tagaaggtaa gaattcttca tgtagcccca tcatatcgcc aaaaggccgg actagtttta
    11521 cattggtatc atcataaaaa tctagcactt catcacaccg gcgctttagt tcatctactg
    11581 attcggcact tactcgaacc acatagctca gtttgtacat ggattctttg gactgatcca
    11641 aggtagtttc caattcatct acggaatcca atgcatctaa gacattggaa tttgtttcat
    11701 tgtcagattg ataggcatgg ttgtccaagt cttttaattc ctttttctta ttgcgaacgg
    11761 tagcaagtgc ctttttgttc gtcacaatct ccacattcat actagtgtca attgggaatg
    11821 taaattgttg ttgctggtaa tagaaaagtt ctgaggaggg aaactccatt tcccccacaa
    11881 ttgtattaat tgttaagtac gctacatatg attcatgatt ctcatgttcc atgcataagt
    11941 accggggctt ttcttcaatc aaacaacgac tgggacgtaa taagtcgtaa cgtttcacca
    12001 aagtttccga cttcagcttt ttctttggta gctgaaattc atattcttca aatggtgtac
    12061 ccttttcgcc atagatatgt tcaatcaaat acatcagatc actcggtgtg actcgtctga
    12121 ctttaaatct tcgagccaat ttactttcca ttagtttttc aagcttggta tatcggcgaa
    12181 tttcttcatt ggataaggaa acaaaatcgc ccatcaaatg atgattcact ccatagacaa
    12241 attcttgaaa gccggagaag aaagatttct tgagattctt tagattgacc tcttcatctg
    12301 tcgcaatcag tttgaagcca ataaaaaagc gataatcaat ctgactatcg ccaatcatgg
    12361 atactaatgc ctctgtctgt aaatcaattc gttgtttcgc tacttctgtt aatcgcccgg
    12421 taatttcctt ttttgatcgt tcttgcgtgg cacgaatact actttctgtg gcaatttgta
    12481 aggcatgaat cttgccttca cgattttgcg caatcaattg acgaaagtta tcgtggactt
    12541 gatatttctg ttcaggtgac aaaaaggagt aattgtaagg tattaactca tagtaggcaa
    12601 aacattctcc ctcttgatta aagacaagat tattttcaat atacttaatc gggtacatag
    12661 ttcacactcc ttaccagagt aatgggttcg tcccatcgtt tcttctgttc tttgactttc
    12721 tttcctgcga aagtgacctt aaatcgtaag tgatagctaa taaaagatcg taaaaatccc
    12781 aatggtttct taccgtcaaa cgttttttgc gacatgaacc aagtaactgc caccggtata
    12841 cctaagtatt ttaaaaaggc cccctctatc atagatagtg gaggcacatg ggcaaatacg
    12901 atgacaaaca gcaaggataa aacaaaccaa gccatctggt taaaagtcac aggaaaaggc
    12961 agttgaaagt cattaattgc ataaatgacc ttttcaactg cccaaatact ggtgtagctt
    13021 tttattttct tcatcaaatc attcctttaa aaagaaggat agtttagaaa tcaactaccc
    13081 ttcaataatt ttgttaatat ggatactcaa aaacacctcg actcgttacc aaataattgc
    13141 ctccaatctc caagtctcta ccacacgcat caaaatcaat atacgtcttc aagtgttctg
    13201 gtacttctcc aaaatcacca gcttctacaa tcaagaaata tgctacatca ctcatatcat
    13261 cgcaatctgg ataatagata atatcatcca catgctctac catctcctca aaagaattga
    13321 agaaagcatg ttgaatctca gaagcaactt caccaactgg cgttccttca agttcctccg
    13381 ctaagccaca caaccgatta atttcctcaa ttggcgtata ctcatcaatc tcaaagggaa
    13441 gttcataatc atgaattgca tattcctcgt attcgtcatt taacccaatt cgttctttca
    13501 cttcatcaaa atcgacaggt ggggtaaacc acgcccccac caattcacct tcgttatatt
    13561 tcccgaggtt cgcaatataa acacgcatct gttccatcgt gcttccccct tacgcaccga
    13621 caattcggtt aaacaaactg agtaagacat ccttcacccc agaagcatta aagaccaatc
    13681 cgaccgcaat caaagcaatc accaaaaagc caatcagttt ggaaaactcc cgcttaaatc
    13741 ctaaaaacaa accaatgacc acaattgcca gtaagactaa ggattgggca ttggttaaaa
    13801 accattgata tatggatagg taagttcctg atattataaa tatctcaaac ttttcccccc
    13861 attcacacgg agcgtgcgac tttcaccgca ctccgcgttc catcgtgttt atattgaaaa
    13921 gtatttatgt agtagaccta tatcaagttt aattcttgat tgccagctaa tactctatat
    13981 ttatttagtc ttatgagctg gactttattt agcttcaata gagctatata tttttgtatt
    14041 gtttcttctg ttttagcgtg tattagacgg tgtaccgcct cttttaccaa aataagattt
    14101 ttataatcgt catttccacc agctccagtt ggtacaatat gatgacaatg tatttcatta
    14161 gcttgttgaa attcttccag agttatagca cattttccat gttgagcaga aaatagcgaa
    14221 attcgattgt ctgcgtattc tattgtgcga ttttccaaag ataatttcat taactggtac
    14281 atcagtaatt tatcaacact tagattggtg tggataaatt ttcttccctc tagagtgtag
    14341 caattcgcta aatacacctt tgccattggt tttctgtgtt ggatatatcc aacagggaaa
    14401 ataggttcgc cctcgccagc aacataccga agcattttag atttaccata tcttgttgcc
    14461 tcctttgtcg taagttgtct gcctgttttt ctcaatctgc cccttttgtt tgtacctaat
    14521 ctgttagtga agatagccat tacagctcta ttcagtttca tacaatccag atttacattc
    14581 gttgcaatct gataataatt ttgcatacct aataccattg aattataaag tcttgtttct
    14641 tctaattccg ttttcccttt tgagggagaa gcaattttct ttgcctgtga taccagcttg
    14701 tctttttggt ttttgaattg tttatcactt atatgtgatt taacaactaa cttctttctt
    14761 ctcggtatca ttttaatctt gaaaccaaga aagtcggagt atctttttct aacatttaca
    14821 attcttgttt tttcttgtga tacttccaat tttaatcgtt gttcaatcca ttgagtgatt
    14881 gctattttgg ctttttcagc actttccttg tagcggcaga aaactctaaa atcatctgca
    14941 taacgaacca tatacatttc ctttaacttt gttttcttca tttctttgta agcgttgctt
    15001 ttacatgggc taccgctttt attgatatga accacatagt tttttgtaac aggattttcc
    15061 tgccattggc tttctaccca atggtctaac tcattaagaa caatgtttgc tagtagtggg
    15121 gatattattc ccccttgcgg agttcctttc tctggatatg ttattgtacc gttagtcatt
    15181 ttgattggag cttttaatat cctttttaaa atgaatatca agtgcttgtc ccgtattccc
    15241 atagcccata tctgttttat cagcttggaa tggttcacgt tgtcaaagaa acccttaata
    15301 tcaaattcaa tcacataatg tagctggctg atttgtaggc ggttataggt tgcttttatt
    15361 gcattttcaa ctgacctatt aggtctaaaa ccatagctgt tttcactaaa tttagcttcg
    15421 cagattggtt ctaatacttg ttttatacat tgttgtataa gcctgtccca catacaagga
    15481 atacctagag gtctggtttt accatttggt tttggtattt cttttcgcct cactggctta
    15541 ggtcgatacc catgtggact gcctgcaaca atgtatctta cttttttgac aactgtttct
    15601 gctggcaatt tgccaatatc tttaattatt gtaccgtctg ttcctgctgt tttactgcct
    15661 gcatttgctt tgatatttcg atatgccagt aatatatttt ctctcgatag gataatatcc
    15721 ataagatgag tgaaaatatc tccattctta cttttggcat ataaatcatc aaaggtgttt
    15781 tgcatttcgt aatattcagc gtgtcttaaa tcttctacac acaatgtttc atttttattt
    15841 ttaggcataa ggcttcactc ctttacttga aattgagtga gcttttttat cttactcgta
    15901 atccttattg aaacaacata atacttttct aatatataca cgacttgagg gcattcctcc
    15961 attctcatta caagaatttc ttaggttcgc cctctgcttt tcagcattag ttcaactgct
    16021 tattgttctt cgtcagttta ttctcttgat gagaacccaa tacctttcct cgttccgata
    16081 atcttatcta tacatatata tccttaggtc actagctata agcctgctgg cttgataccg
    16141 cctgtaacgt tatatggcat ttcataacct atattcttac tttgccacac cagctacgat
    16201 ttctcgtacc tttgcatttc tacaaagttc aaatttagac ccgtacattc gctgtttcgt
    16261 cagttcctta tagaacattc taaccataga tattttatag acccccgacc tgttggatac
    16321 gctacccacc tctggatagt tttcgataga agatatatgt tcatcaacta ttacggtatc
    16381 tttcagccgt ctttaccgag cttcaaacat tctgagtttc ctcatacaat gcttgtcgga
    16441 ggctcagggc aggcgtttca gagcgttact tcatcattcc acctgtcaga ttatcagttc
    16501 tcctaataac caaacttttt cgttctttta ttagtgcctt agcttttcac taaggaacga
    16561 gtcgcacata aattttgtcc gaaattcatc ttgtcacttt cctttctttt ccattgctcg
    16621 atccgctgct gcacttgcat tcatcaagca catcacagta acaccaagca tactgccacc
    16681 aaataccaaa aaaatatctt ttacaattcc catgacgatt cctcttttca tataatcaaa
    16741 ttttcaattg ccatactttg ttgctccaaa atctttttat gtcggtctga taagcgtgca
    16801 ttctgtacca tatcctggat ataggttgtc cgattcaatc gatccaactc cttagccatt
    16861 ttccacgtag gggcgacttg tcggcctagc caattcaagg ttcgttcaat ggtatacggt
    16921 tttggctcgg aagttaattg aatctctttc cgattccgtc caataaaacg cccccattgt
    16981 tcattcaagg gccagttggt tcgcctttta ccttctactt tatccgcaaa tcgcaagtaa
    17041 cgattgataa tggaaaacgt ggtgctctca atgctacgac cttttaacaa atcttgaatc
    17101 gcatgaaaag cacggtcatt tttcagtcga atctcaaaac gatttttcgt ctcagtttgg
    17161 tctaatggaa tccctaactt gatatattgt tcatagtctt tttcatacaa acagaaataa
    17221 acctcactct tgagactgcc gatatataag gtattgccca tgccgtcctt ttcatcagcc
    17281 tttaaaagtt ccccggaacg ataactttta aaggtacgaa acaaggagat acattcttcc
    17341 ttctgacatt ttttcgttag ctcaggtata tccaatagcc ccactcgatc attgattgcc
    17401 aagtccaagc gtttcatcac gccaccggcc tttagacaat cctcgaaaaa gtcgtaccag
    17461 ctacgctttt gagcgagtaa aaaagtttca aattgtcggc aacctcgccc ctttaattct
    17521 aaaagcacac ctttatcttc ttcatgagac aacatcacca caatatcacc catgacatac
    17581 tgttcttgat aggagtaaaa ggcataatct tcatggagca tatactcaaa tttcaaatgg
    17641 agaatttctt gaataatttt tcgtgcatcc gttgtgggaa agcgaatcct tacatagtca
    17701 aacagtaacg tcaaaggcaa atcgggatta aagctttcca gttggtcaaa aattttcctc
    17761 atggttttta tagatgcggt tacgccacca ttttctatct gatttaagta cggccgacta
    17821 agtccacaag ccgtagccaa tttattttgt gaaacgccgt attcgttgcg tcggtctttt
    17881 aatagccgat aatctaaatt ccgttgtgcc aagttctcac ctccaaattc aaggtgttaa
    17941 ctttttcaaa tgagttaaca cctatacaaa ttacttacta tctttgatac aacaagcatt
    18001 cttaatggtt gcttacttat tttttgtgtt tttgtacccc cctgttagat acgggggtga
    18061 gacatcatgg tcggctaacg ccgtccatga acaggcacag caccacggag gatttctgct
    18121 tcgcgaatcc tccgcggtca gcgcctaaca gtcagttgtg ttggtttagg ttactcatct
    18181 gtaataccat ttacttccaa ctctgctttc atttctaaca agtattgacg aatatctggt
    18241 tcttttgctt ggtccgcaat ccgctcaaat tctgcaatta cttgatttat ttcaattcga
    18301 gcatccgtct cctcattcat taaatatgga tcgccagcga ctttgacacc attcttgcga
    18361 atatcaaatt ttaaccgtcg caattgttct tctaattctg ggtctgactg aatctttcca
    18421 atcacccaat aaatttctcc aatcaactca tcataaacca tgtacatcca actcttttaa
    18481 tcttctggca atatcaaaaa tgaccggaac tgttacagag tttccggctt gcttatacaa
    18541 ctgactatct gaattgactt gagaatctcg atcaaaagcc caatcgggaa agccctgaag
    18601 tcgccaacac tctctaggtg ttagcttccg aatttgatac tgatccgtga cgatcgcttg
    18661 ttggtctcct gttagtaagg tatgaacact ctgttttccc agacgacctc ggcgacttgt
    18721 ggaattagga aaagccagat tcacactgtc ttctggcttc acctcttgat attctgtttc
    18781 attttttcga atcatgattt ttgtaccacc ttgtttcgtt gacaaggtgg gggcggttcc
    18841 agtaacttca taaactcgat taccttgttc aaactttccc ggtaatagtc ctgctacaga
    18901 gattccatcc cttgctggca gtgcgatttt gattccttca tcacctgtta aggttggcgc
    18961 tacgccatct gaaccataga cttgtccacc cattcctcga ttggaaggat tgatatttac
    19021 caaatttctg atgttttgat cagatgttga agacggtcca atgataggaa atacttgttc
    19081 ggtacatgcg tctcgagaat gtccgataag gaatatccgc tcccggcttt ggggaatgta
    19141 ggcctttgag ttaagcacct accactccac gtcatacccc aatccatcca gcgttcggag
    19201 gatcgtctcg aacgtagccc ctccttcgtg attgagcaat cctcggacgt tctcaaggaa
    19261 taaaagctta ggtcggagaa gagtggtgaa ccgagcgatt tcaaagaata aagtacctcg
    19321 agtatcggaa aatctttgcc gctttcccgc aattgaaaaa gcttggcacg gaaatccgcc
    19381 gcaaaggatg tccactggtc cgagggattg aataaactca tctgatacac ttgtgatgtc
    19441 atgcatttcc acctccttac ttgtatcatg aattgcttta taactacgtc tagcaaattt
    19501 atcaatttca caaaaaccga tacaatgatg gctcgcctgt tccataccta aacgaaagcc
    19561 accaataccg gcgaataaat ctaaaaatct catacattac ctcattcatt gcaaagaaaa
    19621 aagccaagaa cattttgctc ttggctcata cattattaat ttgtcctact attttttatt
    19681 taatccaact aatttccgtt gtttttcatt ggcctgatcg gctacatatt taatcaccgt
    19741 tttgaactct tttgcagtag cattttccga taaatccgca atgctcttat tgtatttctt
    19801 tacaattttc gttacttgtt ctttctcagt catgtaattg aacctccaaa tctttcctac
    19861 tataccctag tggtaaaaat aattctagat tatccgtagc ataatgaact tcggaaatat
    19921 tattcgtata atcttttatt atgatatgaa gcaatacatc tggatgttta ctctttactt
    19981 ttgcaatgtt ctctcttgat ttgaaaaaag cgttaataaa tgcctttttc ggcacatgac
    20041 gtccctctgc aatttcacgt tcttgtgtaa atttccaagc gattttaggg tcttgataaa
    20101 catagaaaat cgtcactcga aaatgttttt tcaacgcccg cgagatattt ttttcagcac
    20161 ttaatattgc aaaagttgca tccagaataa aagaatagcc atgatctaag acatatctaa
    20221 atgcctgctc taccaaccaa gaagatgctt tttgaaagag actagagttt tttccattat
    20281 aatcaggaaa ctgggtgcga aagtaatccg catcaataat cacatggtta tcataattct
    20341 cagctaatcc aatggcaacc tctgattttc cagccccagg actccctgcc ataaaaactg
    20401 cgtctcgtcc aacttcgggg gatttccctt tgataatata agaaataaac ttatccttat
    20461 ttgcttttgc atactctaaa taagaatcat tactttccat attaattatc ccctttcttc
    20521 ctactatata aaagtattat acgttacttg aagtataaag tcagtattga agatcactca
    20581 atcattcatc ttttctaatt tactaattga atctaagaaa tcataccctt ttggtacaag
    20641 tggcgtataa aactcactaa tcacactgcc acctgtatcc acataacctc gacctttgat
    20701 gtgcttcata aagaagtttt tttcgacctc cccaaacatc atggaatatc cgagttcact
    20761 catacgaccc aaagccacgc gaaagttgaa ttgatcgcgg attccgtctc ctaaatattt
    20821 cgcatccggc ctttggcaag ccaaaatcag aaagaaacca gattgacgac ccaacatcac
    20881 gatttgtttg agcttattca aaatcaccgc actttctttc gtcgttaaca tctccatata
    20941 agccacgtat tcatcaaaga ttaaaaagtt gggtggaagt ccaagatagg cataattctc
    21001 tcctgttttg tagttagaca tttctttcat tgccttgcta cgagccatca tacgttcata
    21061 gaaatcttcc acacaagcag aaatttcttc cttttgtgaa taaacatgag gcatcaccgt
    21121 acctaaatca gctaaatctg catttttggg atcgaggata aacaattctg catctgactt
    21181 caatagtgct tcgataatgg tgaggaggaa ataggtcttc cctccacccg tgccaccagc
    21241 gattaacata tggggtaaag aatcataagc ccatacttga tttttcatca atcgcagagt
    21301 cccattttct gccactactt cgtctatccc aattcgattg gcgatcatat catacagtaa
    21361 agtatactcc acataggagt ctttgagttc cttttctact aactcacaat acagtccgct
    21421 ttctaacttc ttttccaatt ttaaaagctg atcttggtat ttcccaagtg aaatttgaac
    21481 gcgaatagac agtaatccat ctttcattcg ataacagatt ttggggaagt aactgatagt
    21541 ctctctggtt cggctactat tcaaatcttt gaaaaagcct tcactttgag tttgtttcac
    21601 ttcataccaa tggttctcta gaatcatccg agctagcttt tgtcgatgcc acaagcttcg
    21661 ccaatgatca agaaaaaagt gaatataaag caatatcata gctcctgaaa aagcgagact
    21721 tccaagcaga catactaaat cttttataga ccaaacgatt tcaatagtag aaagtgatag
    21781 cttagaaata tcaaattgtc taaactcttg aaaataaaag acgctcatcc aaccgatgaa
    21841 cagagcgagt agccagccaa gaacaaagtg ataaaccaag tgctgatctc ctgcgcgtat
    21901 gcgatgtcct ttgtacattt tgatggtttc cacctccaat cgaaaaagcc caataggatt
    21961 tcattaggca atttcaaaaa gttatgctag tcatttactg ctacgaggag gggagttgtt
    22021 tcgtttcaac caattgattt aaaaagcctt caatagcatc cggcatctcc acaccaattt
    22081 cttccaaaat ggattctgcc tgccgacata agttttcatt tacttcgaca actataaaga
    22141 aagactcttc ttctcctaac cagtctagta catcagagtc ttcttcactg tcgctatttt
    22201 ctccaagtaa accttgatgt ataatcagaa acattttttc atcagaggtt aatgctttaa
    22261 cgagttgttc ctcagtcagc tcgccttttc catattgatc gagacaatct aaaatatgtt
    22321 caacattaat ttccataatc ttcacctatt tctttctttc attttggttc gtaggatttc
    22381 cttgattcgg ctgccgcttc aagactaaat catccgcctt aatgaaccaa ttaacggtag
    22441 ttccttctcg aaaaacataa ttggcaacgg tatccacgat tggattgacc aactctacct
    22501 ctgcatcata agcaaactcg cgtaagggta cattagctgg gatactaact tgaatcattc
    22561 gcccttgtgc agaggatttt aaattatagg ttcgttcttt tacctcatta ccaattgttc
    22621 catcctcatt ttgcttccgt acttctcggc gtaatgcgga aaaacgcaac ttcccaaatg
    22681 ttaaacctga atctacgaca ataccttctg ttaatctcat cttcttttcc tcctatgctt
    22741 tcactaaatc atcaacatac aacacgtaat cagtaaaccc acgattctca atctcatagc
    22801 ctttggcaac caaatgggga ttcacggctt ttagcggttg gtcttgatca aaatcctttt
    22861 caccggcttc cgcagctact accacctcaa tatcatcggc ccgttgctta ctggaataca
    22921 aatgataact gcgaccaatc accgtagtat tccgtccata tccttctgtt ataatttctc
    22981 cttcaccggc ataacttaac tttccaaagg tttcagccat atctggcacg acaaatttca
    23041 attccatctt cttttcctct tttcattgtt agttttggat acaaaaatag cgcgactact
    23101 tttttaggag gcggtcgcgc acgttggttc ttaggattta cttgtatcat ctcatcacct
    23161 tagttccggc gattccagaa ataatagccc gccgtcgcaa aaatcaaagt aaaaccaatg
    23221 aacagtaaaa cattggaatt tttctccccg gtttgaggga aggtcttcac tgtgggacta
    23281 gattcttgag agccacttgg aacagttggt gtttccggtt gtttcggcgt acttagtatg
    23341 gttggaactt cttttggtgt taaggtttga gatttttctt tcaaatcttc attgtgtttt
    23401 ccattgatat tcccatcttt atcgtaattg atttccgcaa aagtaaactt ggttccttct
    23461 ggatattttc cggtatctac ttttttcgca agcacagttt tggtgaattc tttatcattt
    23521 acttcataat caatcttgcc agatttccaa atttctttgt ttgtaccatc cggtaataaa
    23581 gcatacagaa ttgtttcgaa agcttctttt gagccatcca gtacatcatg agtaatcgac
    23641 acatcatcaa acatatccac cacatcacca tgagtaaagg tctgagaacc gttttctagg
    23701 tgggcctttg tttggataga aacatggtgt tccactgtac aactgacagt ttgggcttgg
    23761 ttattcaagc taacatcctt cgctactggc ttatcgcctg cttcaaaagc ttccttattt
    23821 tcatacacat agttgaataa gacaatagtt ttatttaaaa cttgttcggc ggttaattta
    23881 tgctgaattt tccaagttcc tgttttctct ttgttcgcca acgttgcgct tgtttctgca
    23941 atcaccactg gtttggcttt ttcatctttt tcttgggcag cttttgtcgc atccacatca
    24001 atggcttgcg ctaccacata ccagtcttct ttgatttcat gtagattgta actcaattta
    24061 tcaactagct cggtagaatc cagagaagtt aattctttgt ttccgtcttt ccaagtcgca
    24121 agagaagtta aactatcttc ttcctcaggc aaatcataga gcattaaacg gttcaaacta
    24181 acagaaaatg ctttgttcgt cagcttctcg taaggaacgg tcaccgtttt aattggttgt
    24241 ttttggtctt gttcggtaat ggtaaagaca tactcactct tcacaaagtc ttctttgttt
    24301 tccttaaaag tagaacggat ttctaatggc gtaatctttt gaaaaccttc tggggcttct
    24361 acttcttcaa gtaaataatc cccataagga agattttcaa acttgccata accatcaaac
    24421 cctaattgct cgttataagc tgtggtcgct tcatcttcgg cacctgtgat ttcattggtc
    24481 ccttccaatg gcgacacttt aaaggtcaga tcattgaagc cagtttcggt agtgcccgca
    24541 gctgatccag caaatttaaa gaaatcaaag ccaaagcgaa tcacttgttc ttttgccgta
    24601 acatttcggg taatcacggc atttttttcg ctatcatcca cctttttgat ggatacagga
    24661 tactttgttt catcaaggct atatccttcg ggcgcttttg tttcttgcca ataatactcg
    24721 ttgatagcta aatgtttaac ggcaacttgg ttcttttcgt ctaaagtcaa agtgaccgtt
    24781 tcatcagaac ctttcgttcc cttcgctaac tctggtttaa aagcctcact ccatttaaca
    24841 gcttttccat ccttagcagt aaacaaggtg tattcagcac cctcaaagac tgccttacct
    24901 tgtgctttat ccccagtatc tttgtcttct ttcgtcaaag tggtttcgcc agtgacttct
    24961 tgattttgcc cttttacgtt actggtaaca ggggctacgg tttgattggc atacttcaat
    25021 tcgactttta caggtttgaa cgtattcacg aacccatgac tagctttagt ttcggtcaca
    25081 taataggttc ccaattccaa cgcattagca atctcttttg gtgtttccgc atgaccgttt
    25141 tcatccgtgg tcatttcttg aacgatttca ccggttggac tgtctttgcg aatggcaaac
    25201 gtattaccag ctaaggaata attgtcattc caaagatcac tccctgtttc aactccgctt
    25261 ttatccagaa tgatttgccc tttttcccgt gcgttttttg aagtcacgta aatggtttcg
    25321 cctgctttaa tggtggtagt catgggggtg gtatcaatcg tataaggcgc cggtactgat
    25381 ttttcggtaa tggttacttt tgtaccatga ggaatctcat ccaatgtagc aatgccttct
    25441 ttgtcagtag tcacgtcttt tgcaggtaaa gtttttccaa agtctaaatg gaaaaccgtt
    25501 cctggtacaa catcacccga ttctttatcg acttttttga tctttaaaga accctccgtt
    25561 tccacatcaa ttttgattgt gtaggtatta ggtttatcaa tcgccccggc cattaaagtc
    25621 tgttggccag cctttttata ggcgacaggt gttccggtac cagcagattt cttaagcgtc
    25681 agcacaccac tttcttttga attggcattt ggggtgatta tcaactgatt accattcaca
    25741 cgataatcaa tgtttgcggc attttccaca acctcatcga actctgacaa attcagatta
    25801 ttcgtatccg tcacagtagt cgattgccct aatactgttt tagccgtact attatggaag
    25861 gttggtttct tctgataatc gtcaatggct tgatttattt ttgcctcaat ggctgcgata
    25921 tttacggcac taccatctga tcgttttatt gagtgaaggg tatacccatt aacttcttgc
    25981 cagatcatct tctgtgccac aatatgagta tctgcatctg tacctacttt tttccataaa
    26041 acggaaacta atttggcttt atcggacata tctggtaacg ggtttttctc atagccagga
    26101 gtgaactcat tgtaaatcgg gactcctggt tcaatacaga aaatatcttg tttaaagccg
    26161 ttgtacactg cataatgcgg aacagtgact tctgacattg gacgaccatc actaaaagtt
    26221 ccttcaacag catattgatg tgctaaatct agttctacgg ttaccgtttg tggatgggta
    26281 atttcatcag caaaagtaat cgtaggagca attgctccaa caattgcttg aacagccaaa
    26341 ctcaaaatag ttaatacaca taatttcttt ttttgttttt tatacatgaa atgttctcct
    26401 ttcaaattaa gcacaaaaaa ataagatgct cgcttgaaca tctttgttta taaagtcttt
    26461 ttaaaatttt tattgactga atagaatgga tttgagaatg aatgagccta aaaatttaat
    26521 ctaaaatacg aaatatactg cctttaaatt agaacacatt ttagaacaca aacatttcaa
    26581 atttaatcaa atataataaa atatatttac gggaaaacct ttataaatca aggtttttca
    26641 aacttattca atgatacgaa gaaaaaacca ggtcttaaga aagcccgtaa agcttcacag
    26701 ttctcaaaac gttaattgcg attatcgttc gagttcacga gttacgagaa tcgtactggg
    26761 cgaagcaatg ctgagcgcag ttaccttaat cctaactttt ctggcacttt ccacaatttg
    26821 gaaagtgcct tttttgtgtt tttagctatg ttaataggta ggattcttgg tctataatat
    26881 cattcgatct ataaacaaac gggaaaaaga taaagtatca tcaaacaata ttatttatcc
    26941 gcttctgagg ttgtgacaga agtggtcagc ttcaagaaat aagaaggaat ttacaaaaat
    27001 tgcttctcat gtttgataaa tattgtgact tgtgaccgag gagttggcag gagaacaccg
    27061 tttattcgga agaagggatt gtgaaaagtc ttttgtcata acctcttatt tggtagctaa
    27121 ttgtaaatcc caaacggcat gtattatttt tcataagtcc gtttttaagg attaatgttt
    27181 tcttttgctt tttatatggt ctggcttcag cgagaagttc caacgcatat acaaggacga
    27241 cttatgtcaa cgataacaac gattaccatg gtggccagtc cacttggatt agcactgttt
    27301 ggtgcgctct ttgattggaa gacaacgcaa ccacgtctgc gagacagtct aatttttctg
    27361 ggagggagtc ttgctatagt tatccttata accggtctgg taaaattact caagcttgat
    27421 ctgaaagaag caaaaatttt cagtgaagag gaatacagag aaacgacttt ggtcgaaaag
    27481 aaagaatcag gaaactgata caagcgtata acaaactgcc tgataatagg aaagacacgt
    27541 attgtccgat tgtttttttt gacattcaat tggaaaattt acactttctt tatacatttc
    27601 tggctgcttt ctttataccc gtcaactata cttcactcat caataagaaa gaaggataag
    27661 tatggatttt ggaaatgaaa cggtcttgaa agcaactggt ataaccaaaa aatacggtgc
    27721 tgccaaagca ttagataaag tatcaatcga aatcaaacgt ggaatgatct atggattgat
    27781 tggggaaaat ggagcgggta agtctacctt tatgagaact attatgggct tgatctcgat
    27841 tgatgaaggt tcgatcgaat tatttggaac gacggatttg caggcagcaa gacgacgtat
    27901 gggtcaatcg attgaaaccc ctgctcttta tccagaatta actgcaagag ataacctacg
    27961 tatccaagca gcgaacggcg gtgtgtcgga tagagaaatc gaagacctgc tgaaaatgat
    28021 gcgattggaa aatacaggga aaaagaaagc aaagaatttc tctttaggta tgcgccagcg
    28081 tttagctatt gcaaatgcac tgatcacgaa tccagaattt ttgattttgg atgaaccgac
    28141 gaatgggatg gatccagcag ggatggcaga aatgcgagaa atcatccaac gtttggtaaa
    28201 agaacggggc atcaccgtct tgctttctag ccacttgcta gatgaattgt cacagatcgc
    28261 cactcactat ggtattttgc atgaaggaca cttgatcaaa gaattatcga aagaagaatt
    28321 ggcacaagaa tctcgtcaat ttataaaaat tgacacttcg gcgacggaac aagcagtaac
    28381 ggttctcgac tctcttggtt atagggatta ttttgtacaa tctagccgtg ttatccagct
    28441 ttttgaagga attgaccaag tagcagccat caatcaagca ttggtagaag caaaagttcc
    28501 tgtggatggt attcacctag ttgggcaaaa actggaagat tatttcttac aattaactgg
    28561 aggcaatcca catgtttaat ctattaactg ctgaaaaaat aaaagtatgg aagagccgta
    28621 aaatgtgggt ctcactagga ctgatggtcg tacttccgct tttttattct tggaacgaat
    28681 ggtatatgca tacaaaatac ggcaatgaat taaaccaagc aacggataca gtcatcaacg
    28741 gtgcaacagg gattttaatg gtagaaaaaa tggctgcatg gattcttctg gctttcgcag
    28801 catttgcgtg cttttatatt ggggaagaat ttcaaaacgg tacgattcgc aatacgttat
    28861 ctttaggacg gaatcgaatg acttattatg tgtcgaaact tctcgttacg cttttgatca
    28921 caacacttgg cgtggttttg atcacgggac tagccatgct tgcttacaca cttgctttcg
    28981 gatttggtga agttgaggga atcaaaaact atggaaatta tgttctaaaa gtatttcctg
    29041 tattgcttct gctgacatta gggacattat ctgttccagt ggctttgaca ttcatcacta
    29101 gaagtaccag tgtcagtttg cttctttctt ttctttatat catgggaacg gccttcattc
    29161 ccggtgtttt cgcaaaaatc aaaggcttgg aatttctaac ggagtggttt acggaaacgt
    29221 ggctcatgta tacggacttt gcgcaacaag caacttattc gcaagcaccg aaaatgattc
    29281 tggtcagtct agtaacaatc gtggtatcat cagctctagg aatgtttatt ttccaaaaat
    29341 ctgacattaa gtaggtggtc ctgctggaaa ctattttgat tatcgaagac gatgaagctg
    29401 ttcattcctt actggaggag gttcttgaac agagatacaa acttcttgat gcttattcag
    29461 gtacggaagg aaaattattg ttaagcacat atcctgtcga tttgatcctt ctagatctca
    29521 tgcttccagg gctttcaggt gaagcattgt tagcagaaat ccgacagaca tcgaatgttc
    29581 cgatcatcgt tctttcagca aaaagcgatc aaagagataa agtttccttg ttggcagcag
    29641 gagcggatga ctatgtgacg aaaccatttg atatcgaaga acttttgtta cgaatcggca
    29701 tccaacttcg tcatcagact tctgtccaag taaaagatct gtcgcaacgc atctattaca
    29761 aggaaatctt actggacaaa gagtcgaggg atgtaaatgt gaatggtgtg gctgtccatt
    29821 tgacaggaag agaattcgat ctgctgaagc tttttttaga acatccgaaa aaagtatttt
    29881 cgcgagcaaa tctttatgaa acagtttggc aagaaccata ttttgatagt gagaaaacag
    29941 tgaatatgca tatttccaat cttcgaggga agctagcaaa ggcacatgct gtgtatattc
    30001 atacggtgtg gggagtgggg tttagatttg attaaatggc tgttgcttct cgttgttatc
    30061 tttatgggaa gttttatttt cttcctgcaa agggaaatca agagattaac taagaaaatc
    30121 gccactcttt ctgaacgaca agaatatggt ggtcgtcttt cagtcgaatt tcgagaaaag
    30181 aatctaatgg acctagtaga tgcactgaat acatggttgg atgaaaatga acaaaaagga
    30241 caagcatttc aagaaatgga agaaaacatc agactatcga tcgtcggcct ttctcatgat
    30301 ctcagaactc ctttgactgc tattaacggt tatgtacaat tactggaaca ggtagaggat
    30361 acggagaaac gggagaagta tttaacgatc atcaaacaat ctgttgcaag attactcagt
    30421 atgacggatc agttttatga tcttgctcga atagagacga agcaaaaaga aatggatctc
    30481 catacgcttt ctttttctaa agaggtggaa gaagtatttt tctcttttta tgaacaattt
    30541 tctgagaaaa atatcgaggt agcgttcgct gaacactcga taaatacaac tgtattagca
    30601 gatccgctca tgttgctgcg agtgctgcaa aatatcatac agaatttatt gcgttatgca
    30661 gatggacaag caaaggtgac attttcaaaa gaggatccat tcattcgatt aacagtggcc
    30721 aatcctatca aaccaggaac gcaaacctct gttgaaaaag tcttccagcg tttttatacc
    30781 gaaaatacca gccgcacaaa tacggaatct agcggaatcg ggctttacct gtcaaaaaaa
    30841 ttggtggaag gaatgggagg agagatgacc gctagattag acgggggcat cttctccatc
    30901 tccgtcaagt tgcggagggt ttaattatac aagattagtg agaactagta
//
LOCUS       DACCXO010000022        30834 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.023, whole genome shotgun sequence.
ACCESSION   DACCXO010000022 DACCXO010000000
VERSION     DACCXO010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.023"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            111..1247
                     /locus_tag="IVY70_000953"
     CDS             111..1247
                     /locus_tag="IVY70_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP7958097.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1384..1728
                     /locus_tag="IVY70_000954"
     CDS             1384..1728
                     /locus_tag="IVY70_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP7958098.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            1999..3285
                     /gene="efmA"
                     /locus_tag="IVY70_000955"
     CDS             1999..3285
                     /gene="efmA"
                     /locus_tag="IVY70_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP7958099.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3423..4022)
                     /locus_tag="IVY70_000956"
     CDS             complement(3423..4022)
                     /locus_tag="IVY70_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP7958100.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            4426..5547
                     /gene="mnmA"
                     /locus_tag="IVY70_000957"
     CDS             4426..5547
                     /gene="mnmA"
                     /locus_tag="IVY70_000957"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP7958101.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            5793..7214
                     /gene="arcD"
                     /locus_tag="IVY70_000958"
     CDS             5793..7214
                     /gene="arcD"
                     /locus_tag="IVY70_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP7958102.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            7228..8346
                     /locus_tag="IVY70_000959"
     CDS             7228..8346
                     /locus_tag="IVY70_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP7958103.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8406..9698)
                     /locus_tag="IVY70_000960"
     CDS             complement(8406..9698)
                     /locus_tag="IVY70_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7958104.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            9848..10408
                     /locus_tag="IVY70_000961"
     CDS             9848..10408
                     /locus_tag="IVY70_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7958105.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            10537..10914
                     /locus_tag="IVY70_000962"
     CDS             10537..10914
                     /locus_tag="IVY70_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP7958106.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            10847..12919
                     /locus_tag="IVY70_000963"
     CDS             10847..12919
                     /locus_tag="IVY70_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7958107.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(13130..14077)
                     /locus_tag="IVY70_000964"
     CDS             complement(13130..14077)
                     /locus_tag="IVY70_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7958108.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            14323..14787
                     /locus_tag="IVY70_000965"
     CDS             14323..14787
                     /locus_tag="IVY70_000965"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP7958109.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(14849..15892)
                     /locus_tag="IVY70_000966"
     CDS             complement(14849..15892)
                     /locus_tag="IVY70_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP7958110.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(16269..17234)
                     /locus_tag="IVY70_000967"
     CDS             complement(16269..17234)
                     /locus_tag="IVY70_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP7958111.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            17468..18313
                     /locus_tag="IVY70_000968"
     CDS             17468..18313
                     /locus_tag="IVY70_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7958112.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            18504..19691
                     /locus_tag="IVY70_000969"
     CDS             18504..19691
                     /locus_tag="IVY70_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP7958113.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            19697..20053
                     /locus_tag="IVY70_000970"
     CDS             19697..20053
                     /locus_tag="IVY70_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP7958114.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            20055..20393
                     /locus_tag="IVY70_000971"
     CDS             20055..20393
                     /locus_tag="IVY70_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP7958115.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            20788..21822
                     /locus_tag="IVY70_000972"
     CDS             20788..21822
                     /locus_tag="IVY70_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7958116.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            21815..22501
                     /locus_tag="IVY70_000973"
     CDS             21815..22501
                     /locus_tag="IVY70_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7958117.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            22525..23373
                     /locus_tag="IVY70_000974"
     CDS             22525..23373
                     /locus_tag="IVY70_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7958118.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            23570..24343
                     /gene="sufC"
                     /locus_tag="IVY70_000975"
     CDS             23570..24343
                     /gene="sufC"
                     /locus_tag="IVY70_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP7958119.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            24356..25642
                     /gene="sufD"
                     /locus_tag="IVY70_000976"
     CDS             24356..25642
                     /gene="sufD"
                     /locus_tag="IVY70_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP7958120.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            25639..26874
                     /locus_tag="IVY70_000977"
     CDS             25639..26874
                     /locus_tag="IVY70_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP7958121.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            26861..27331
                     /locus_tag="IVY70_000978"
     CDS             26861..27331
                     /locus_tag="IVY70_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP7958122.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            27336..28727
                     /gene="sufB"
                     /locus_tag="IVY70_000979"
     CDS             27336..28727
                     /gene="sufB"
                     /locus_tag="IVY70_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP7958123.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(28825..29967)
                     /locus_tag="IVY70_000980"
     CDS             complement(28825..29967)
                     /locus_tag="IVY70_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7958124.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
     gene            complement(30018..30815)
                     /locus_tag="IVY70_000981"
     CDS             complement(30018..30815)
                     /locus_tag="IVY70_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7958125.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAYKDISKYSSVED
                     FFYKITKNQFETEKFISDLSKKYNFPYEKNSIIAKFLTDNHDKFKNN"
BASE COUNT        10023 a         5631 c         6514 g         8666 t
ORIGIN      
        1 ttttttctat gcccatgaaa cgtcctctag acaaaaatga gctcttcttt ggtttgttcg
       61 ctattttatg ctagaatgtg cctagcaata atttggaagg gatgaatgaa gtgaaaacta
      121 tttatctgga tcatgcggca actaccccca tgcatccaca agtaattgaa gcgatgacgg
      181 caatcatgca agacacgttt ggaaatcctt caagcataca tggatttggt cgccacgcgc
      241 atgaaaaatt ggaaacagca agacaaatca ttgcagacag tctgcaagca aaacctcacg
      301 agatcatctt taacagtggg ggaacagaag gggataatac agctatctta gaaacagcgt
      361 tctcgcgtca aaaagaaggc agacatctaa tcactacagc tatcgaacat cctgctgttc
      421 ttaataccat gaaatattta gagacaaagg ggtttgacgt tacatatctg cctgtagatg
      481 aaaaagggaa tctgtcgatc gaagaggtca aaaaagcgtt acgagaagat acgatcttgg
      541 tctctgtgat gtacgggaac aatgaaatcg gaaatttgat gccaatcaaa gaaatcggtg
      601 aacttttagc ggatcatcca gcttatttcc atacagatgc ggtacaagcg tacggaaacc
      661 aaacgatcca tccagaagaa ttaggaatcg atttattaag tatctctgct cataaaatca
      721 atgggccaaa aggcgtcggt ttcttatata aacgagacgg agtaaatatc cctgcattgc
      781 ttttaggcgg ggagcaggaa gaaaagcggc gtgcaggtac agaaaattta gcagggatct
      841 ggggtatggc aaaagcagtt gaactgctgt cacctgaaga acgtgaagca cgtaacaaca
      901 aatataaagg attccagcaa ttgattcttg atacattaga agcaaacgaa atcccttatc
      961 aagtaaatgg tgatttaacg aataaactcc ctcatgtgtt gaatcttcgc ttaattggcg
     1021 aagcaaatga tttgctactg atgaagcttg atcttaatgg ggtcgctatt tctactggtt
     1081 cagcttgtac agcaggaaat atcgagcctt cacacgtatt agaagcaatg tatggagaag
     1141 atacaccgat actaaaagaa tccatccgta tcagctttgg tttgggaaat accgaagacg
     1201 acatccagta tttccttgat caattagttc gtgctgtcaa aaaataacta gtgaagtcat
     1261 tcttatatga acagatgtct gagagaacgc cttcattttt tagggaactt aactgaaaaa
     1321 cctagtaaaa atcaggataa acttttataa taaagagaaa ctaattttat cgaggtgaaa
     1381 atcatggcat ttgaaacaac agcaacggta ctgggcgcac caattagcta tcgtttgcat
     1441 cctgatgcaa aaagatatac attgcgcgat aatggattta cagaaacaaa cggaggcaat
     1501 tatcaattga tccgaccatt agatgccacg ccgcaaagca aagaaggatt caaattaaaa
     1561 atcactgtat caaaagatat tcaaacattg aaaatgtcga tcactacagc aaatggattg
     1621 aaagcagtca atattttcaa agatccaaaa cagaaaatga gccaagataa attttatttt
     1681 ctgatggatg ggatgattag ccgtgggtta ttcgaaaaag tcgaatgatc tgtggatata
     1741 aaaagacaac tagttttgaa acggcctatt gttagatgac taactttgta aggacgacgc
     1801 aaattagttg tcttttttat ttttaaattt aacacatgtt gttattttta atattttgct
     1861 gatttattgt ttctaagtaa agaaatttaa attgaaacga ttgttattta gggaaaaagg
     1921 tattttttga aaaaagaatc ccgtataatg attgttgtgc aaacgctata caaaatgaaa
     1981 aagaaaagag ggatgtttat ggaaaatgaa cagtcggtcg ttttaacgaa ttggaagaga
     2041 aattatttgt tctttttatc gggacagttt ttgtcaggaa tcactagtat ggtcgtccaa
     2101 tatgcgatca tctggtattt gacaagagaa accggttcgg cgacaatttt gagttttgct
     2161 actttactag gaatgattcc catggtttta ctaagcccat ttgtaggacc gctagtggat
     2221 cgctgggata aaaaagctct tttgattgtc acggatatta ttgtagcgat tttcgcgctt
     2281 attttagcag tagtcggaac gatttcagaa tccttcccga tttggctagt ttttgtgtct
     2341 ttgtttatgc gttcggtcgc acaaacattc caaatgccaa cgatccagtc gattatgccg
     2401 acaatcgttc catcttctca cataacaagg acgaatggac agttaggaat ggtccaatca
     2461 gcgaatttca tcattgcgcc agctctaggt gcggctttgt tttcagtcgt tcctgtcaat
     2521 taccttattt tattagatgt attgggggca gtttttggtg tcggcttatt gatttttgtg
     2581 aagatcccaa aagtttctcc tgaaatattg gaagttccgc ttactatttt taaagatgca
     2641 aaatttgggc tgcagcaatt gatggataat aaagggttat ggtacataac gatcaatggt
     2701 gcatttgtga tgctgttgtt catgccagca atcagtctgt atccattgat gacgctagat
     2761 tactttggtg gttccgttgg acaggcggga gcagtggaag tagtctacgc agtcggaatg
     2821 ctcttaggcg gtgcactgat cagttttata ggaacatgga aagaccgaat gaaacctatt
     2881 attatagcct acatcattat gggcctgacg atcggtgcaa gcggattggt tccaaacgat
     2941 agtcaagggt tcttgtactt tcttatcttg aatgcaggag caggttgtgc aacgccatat
     3001 ttcaatacac tgctgatggc aatgatccag cagagttatg aatcgaatgt tttaggtcgt
     3061 gtcttaggga acttcaactc tttgatgaac ctggcaggtc caattggatt attatttgca
     3121 ggaccattag ctgatcgttt aggggtagaa aaaatgttct tgttttccgg aatcgggatt
     3181 ttattatgcg gaatcgtttt atttttaacg tcagctgccc gaaattatga taaagaatta
     3241 cagaaaaaac tggtaaaaga acaccatgag caaaaagacg aatagtatgg aatggagttg
     3301 tgacagaagt cttgttacaa ctccatttct gatcctaatg ttcctgtggc aataagccta
     3361 ctcaaatgaa aaatatgagt aattatcttt atttgtttaa tacttgatat agggcagttt
     3421 tttcacgacc tatcgtactt cctctttgct tcatagattt ctttgatttg ttgctcagat
     3481 aacggagtgc caaatggtag tttttccttt aagagttctc tttccggtgc gtctacgcct
     3541 acattgacat cgttctcgat aggtacgctg taatgatgga tatgtccatg aagattgcgg
     3601 atagtgtcgt taggcccgag caacatcgga taatgagtca agtaatattg ttgatggtcg
     3661 aatttaacga tggcacctac atcataaaaa taaaatttgg gacttccgtc agtaagccta
     3721 ggatcatttt tttctaaata acggaaaaaa gcgcgactgt catggttccc tttgatcaag
     3781 tggattgttc cttgcagttg agtgaccagt tccaaaattt ctgcatttgc tttttcatat
     3841 tgcggatgca tagcaaggtc gcctagatga taaacatggt cggtttcttt tattactgca
     3901 ttccagctag tgatcaactg ttggttcatt tcatccacat ttgcaaagag ccgcggagca
     3961 aagtcgtttt ttcctaataa gcgttcatgg aaaaagtgca tatcactgat aaagtaattc
     4021 atgtatcttt tgcctccctt ttttcttaga gtaaacaaaa tcacttatct gagcaactca
     4081 taacatctaa aaagaaaagg ttgtaaatga gacgtgcagc tttaagcgat aagaagcaat
     4141 tttgaaaaat agtttttcac aatttataaa attattcctt attgccgaaa ggctggccct
     4201 ttttatgagt atgccaattt tttatatcga atgctcaaaa gatgagtgat tttttggttg
     4261 actatcatgt ttgtgtactg aataggtcca gtagaacagt atttttacga cttgataatt
     4321 aagggatgaa attttcagaa atatgacgta taatgtaaaa tactgaaaag ttgcgacctt
     4381 caactcgtag atgtttcaga atctaattga aatgatggtg atatcatgac agacaacagc
     4441 aaaacacgcg ttgtcgtagg gatgagcggc ggtgtcgact catctgtaac agcacttttg
     4501 ttaaaggaac aaggatacga cgtgatcgga atattcatga aaaattggga tgacacggac
     4561 gaaaacggtg tatgcacggc cacggaagat tataaggatg tagcgaaagt agctgctcaa
     4621 atcgggattc cttattactc tgtgaatttt gaaaaagaat attgggatcg tgtatttgaa
     4681 tatttcctag ccgaataccg cgcaggacgt acacctaacc cagacgtgat gtgcaacaaa
     4741 gaaatcaaat tcaaagcatt cttggattat gcaatggatc ttggcgccga ttatgtagct
     4801 actggacatt atgcgcaagt cactcgcgat gaaaacggcg tggtccacat gcttcgtgga
     4861 gtagacaata ataaagatca aacttacttc cttagccaat tatcacaaga acagctttca
     4921 aaaacgatgt tccctctagg cggcatggaa aaatcagaag tacgggcaat tgctgaacgc
     4981 gcaggccttg cgactgctaa gaaaaaagat tcaacgggaa tctgctttat tggtgaaaag
     5041 aacttcaaac aatttttgag caactatctg ccagcaaaaa aaggaaatat ggtcactctt
     5101 gatggtgaag tcaaaggaca acatgctggc ttgatgtatt atacgattgg tcaacgtcaa
     5161 ggactaggga tcggtggcgg aggagattcg caagaaccct ggtttgttgt aggaaaagat
     5221 ctagcgacaa acacattata tgtggggcaa gggttccatc atcctgcatt gtatgccaca
     5281 agcttggatg caagtgaaat ccactttacg acgaatgaac caatgccaaa agaattcaag
     5341 tgtacagcga aattccgtta tcgtcagcaa gacgttcctg tgacagttcg cttattggat
     5401 gataatcgtg cagaagtcgt attcgacgaa cctgtacgtg caatcactcc tggacaagca
     5461 gttgtatttt atgatggaat ggaatgtctg ggtggtggtt tgatcgacca cgcatatcaa
     5521 gaaacaaaag ttttacaata cgtttaagcg atattaaatg aatcaaaaca gctatggcta
     5581 acaaaagaaa caccttttct tttggatagc atagctgttt ttttgcaata aaattaattt
     5641 ttattcattt tatacttgaa atacttgtgt aaaaagttca taatatccaa taatatgaat
     5701 aaagttgctt tttgttaagc aagagcagtt gcaaaataga agggaatctt ttgcaagtga
     5761 tttgttcaat tttataagtg agggtgatgg aaatggaaaa gaaatcgacg ggtattagca
     5821 aatggggact ggttgctctg gttgtcagtt cgtcaatcgg aagcggtgtg tttgggatca
     5881 caagtgatct ggctagttct gcagctccag ggccagcgat cctttcatgg ataatcgttg
     5941 ggattggtat tttagctttg gtattatcgc tgaatcattt aggagaaaag cgtccagatc
     6001 tagatggtgg tattttcggt tacgcggaag cttcatttgg taaattaggt ggcttcatca
     6061 gtggttgggg ttactggttg tctgcgtggc tgggaaatgt tgcttttgca acgatgctga
     6121 tgagcgccat gggagaattt ttcccgatat tcaaaggtgg acaaaatgtt ccttctattt
     6181 tatttgcaag tatttttatt tggggtctga ctttattagt aaacaatgga gttgaaggag
     6241 cgagcgtcat caatacaatc gtgacgatct gtaaattagt gccattattc ctatttttaa
     6301 tatttgtatt tatcgcattt aaagtgaata tattcactgc tgatttttgg ggtaacgtat
     6361 ctgataatct agtgaatgga acagggcagc aaggaacgat ctggttgcag attaaaggtt
     6421 gtttgatggt tatgatgtgg gtctttgtag ggatcgaagg cgcaagtgtt ttagccaacc
     6481 gcgcgaagaa acgttcagaa gcccagcaag catcgatcat tggtctgctt tgtttattgt
     6541 ttatctatat ccttgcttcg atcttgccat acggcgtctt atctcaagaa gagttagcaa
     6601 ctatttcaca acctgctatg gcaaatattt taaaaggaat cgtgggaaat tggggagcag
     6661 cgctgatcaa tatcggcttg attatttcga tcattggttc ttggttgtct tggacgatgc
     6721 tcccagcaga aacaacgatg ttgatggctc gagacggtct gttgccgaaa ggttggggaa
     6781 aaacgaataa aaaaaatgcc ccaacttatt cgttagttgt tactgcaatc ttgactaatc
     6841 tattcttatt gactttctta gttactgatt atgcctatca atttgcctat tctttatgta
     6901 cagcggcaat tttgatttgt tatctacttg tcgggatcta tcaagtccag ttttctttaa
     6961 aagaaggaat caaaaaacaa gtagtgatcg gtgggattgc tgtagttttt gaattgatgg
     7021 gcatcatttt agcaggattt tcttatgtcc ttttatgtag tattgcctat cttcctggtt
     7081 tttgcttcta ctggatcgct tgtcgtgaaa ccaaacatca aattacggga aaagaaaaag
     7141 taatgattgg gctgatttgt gtggccgcac tgctttcaat cgggttgctt gctgtaggct
     7201 ggatcaagat ataaggggga aagaaacatg aaaatcgcaa atttcggtgt agaagaatgg
     7261 ctaaataaat gggaaaagaa agcggtatat gatattgcac aaagctcgat cgaagcattg
     7321 acattagacg aactcgttgg cttagatggg actagcgtaa gtaaattttt cgaaaaacgc
     7381 aaaaacgaac cattggacta tggctggatc gaaggatcag atgttttcaa acaagaagtc
     7441 gcttctcttt atcaaaccgt cgatccggaa aacatcttac aaacgaatgg agcgactggt
     7501 gctaatcttt tagctctata cgcgttagtg gaagcaggag accatgttgt ttcgatgctt
     7561 cctacttatc agcaattata tgacatcccc aaatctttag gtgccgctgt tgactttgtt
     7621 catttgaagg aagaagaaga ttggcagttt gatttagaac agctagaagc cttagtcaaa
     7681 cctgaaacaa aaatcatctg tttgaatagt gcaaacaatc ctacaggcac attgctagat
     7741 cggaaaactt tagaaaaaat cgcagaaatt gctcgtacag tagatgcata tgtattgata
     7801 gatgaagtat atgcgccttt aacggataaa ggtgaattct tgtcgatcgt agatgtctat
     7861 gaaaaaggta tagcaaccaa ttccctatcc aaaacctact ccattccagg tattcgtatt
     7921 ggctggacag ctactggacc tgagttggca gaagtttttc gaaagtatcg agactacacg
     7981 atgatttgcg gcggagtcct gtcagatgat cttgctgtac atgcattgaa gaataaagaa
     8041 aaaatattgg cgcgaaacaa aaagatcatt acagagaacc ttgtgatttt aaaacaatgg
     8101 gtagccaatg aaccgaaagt tgaacttgtc gctcccaatt acgtctctac ttctttcatt
     8161 aaattagcca tcgaagaaga tgaccagaca ttttgtatca atttattaga agaaacagga
     8221 gtactattag taccaggaag tgcctttgat ttaccgaagc atgcccgact aggttactgt
     8281 tgtaaaaaag aaaccttgga aaagggatta ttcctcttat ctgagttctt gaaaaaacag
     8341 gcctaaacaa aaacactgca aggacagtct gtgacaactg ttcaagcagt gtttttcatt
     8401 tacgcttatt gtctatgaga aggagagtgc cctgaagcta tggagactcc tgaagctttt
     8461 tcttctgcag tttcttgctg atggatctct tgtgtattgt gtcgtacaag caagaagttg
     8521 accaaaacaa gaagagaagt cgaaagaaag acgccgcgat aaccaaatgc actggaaaca
     8581 ctggagccga tcataggccc tactacgtta ccagttgctt ggaaagattg gttataactg
     8641 aagatccgtc cagcagcttg atgaggcgaa tattttgtga tcaatgcttg gacagcaggg
     8701 agcagacaag catcagagat accaatcaaa aaacgtaagg cagctaattg ccaaacattc
     8761 gtgacgaatg ccattgggat ataaaccaga atcgcaaaac ccaaaccaat cgcaagtatt
     8821 ctttcactcc caatcttgtc acctaagcga ccaaatcttg gagcggctat caaagtggca
     8881 atcccaggta tcgaagcaat gattccgctg atcaacgtaa cattcccatg tccatgcagc
     8941 aattggcgaa tgtacagact gatgattgga ctaatggaat tgttggatgc ttggatgatc
     9001 atcgttgtga cgaacatacc gatgacgacg tgtggatatt tcaagtcttt gaaaatctgt
     9061 ttggcagaaa ccatgttttc ttttttcact gggacaaatt tttcatggac gaaaaacaga
     9121 ctcaacaaga agacgaaaaa taagatgatc cctgtaatga aaaaggtagg gcgatagcca
     9181 aacgctgaag cagagatccc gccaagaagc ggtccaagta atgttcctgt aacagaacca
     9241 gttgctaagg ttccaagaac ttgaccactt ttttcttttg gcgtcccagt tgcgacaagg
     9301 gcagtggcat tactgatata accagaaaaa atcccttgaa gcagacgcaa tgcgactaat
     9361 tgatacacac tagtcacgca acccatcaaa gaaatgacga ttgccattcc taatgaagca
     9421 cgcaacagca tcaattttct tcctttttgg tcggctaatt ttccccacca aggagagata
     9481 agcgtagtca ccaagaaagt agatgaaaaa gtcaatccgc tccaaaagtt caattgtgac
     9541 gtactataat tacctaacgt atctatgtaa agtgacataa aaggcatgac taaactaaat
     9601 ccgataccag ccataaatgt tccaaaccat aagacaaaca agttttgttc ccaagtttct
     9661 cttttcctaa ataatttttc ctttattctt gcagccaatt agactccttc tttctgaaaa
     9721 ttttcatcta cagctcttta ttataaacta caatatgtag tttatcaatg aaagtctctt
     9781 ttttctcttt ctttttcgtg gtaaactgaa agagtgaagg atttatttta tgagaggaaa
     9841 catggaaatg aaaaaaagaa atctgtctca agagaaaatc atcgactgct ttcgagaact
     9901 agcagaagag atggatgttc agcaaataac ctttcagcat ttagccaaac gcttggatat
     9961 caaggctccc tcgctgtata accatttcaa aaatattcgt gatgtcaaaa cagcgctaac
    10021 agcgaaactt ctgcaggaac ttaatgacca gctgcgacgg gctcttgtgg gaaaaagtgg
    10081 tcaggaagca ctgcagatct atgcagaaac ttatcgatcc tttgcttttt caaatcaagc
    10141 agtgtatgag ttactgatca gtgtaccgca taccaatgaa gctgttttat tagatggaat
    10201 ccatgaaacg aatcagatca ttttgcagat atttgaggct tttcaattaa cgaaagaaga
    10261 aaaagttcat cgaagcaggg aacttcgtag tatgatccac ggttatcttt ccttacgttt
    10321 tttaggatac tttacaaaag aaccaacaat cgatccagaa gacagctatt tttggatgat
    10381 caataacttt atcgctactt tgccttaaac aatcagattc atttgtaaca attttaagat
    10441 gttcattaaa tgaccctttt ataagagaaa attcttgcaa ctttcttgaa aagtgactaa
    10501 gatgagtcta gaaaagctga aagaaggagc acgagtatgt atcaagtaat taccatgttc
    10561 ggggacaatg agccttggtg gttttttgaa gactgggaag aagatattga agaagaagaa
    10621 acattcgagt ctttcgaaga agcacgacaa gcgtacgaaa aacaatggaa cgaaatccat
    10681 caaaattatg aatatatcaa tgcaaaaagt aactttctca gcgccttttg gaatgacgga
    10741 gatgagcgct ggtgtgaaga gtgtgacgat gatttacagc aatacaaggg acttgcgttg
    10801 ttgaaaaatc atcagcccat cacagtagaa agcagaaagg aattttatga aacaactaat
    10861 tctagcggaa aaaccaagcg ttgcgaaaga cctaagcaag gtgcttggtg ctaatcaaaa
    10921 gcacaaaaat tattatgaag gacccaaggt gatcgttact tgggcccttg gtcatttatt
    10981 aggattgaaa atgccggaag atctgaataa ggaatggcag agctggcaaa tggacacgtt
    11041 gccaatgatc cctaaaaaac taggaatcaa accacttcct aaaacaggac atcaattaaa
    11101 agcaatcaag cagctggctc agcgaaaaga tgtctcagaa gcagtcattg cgacagatgc
    11161 cggaagagaa ggggaacttg ttgctcgctg gatcttagag tatgtgcatt tcaataaacc
    11221 agtgaagcgg ttatggatct cttctcaaac agataaagca atcaaaactg gattcaagca
    11281 actaagacct gcgaaagaat atgatgcatt gtatgaatct gctcttgccc gggcaaaagc
    11341 ggactggctg gtaggattga atgtgaccag agccttaact gtgaagtacg cggataattt
    11401 atcagcaggc cgtgtgcaga caccgacact agcattagtg agagaccaag aaaagaaaat
    11461 cgagcagttc cgtccacaaa cgtatttcac gatcacgttg caagctggaa aagaacaagc
    11521 caaaatgctt caaaaaaatc aatttgcttt aaaaacacaa gaagaagcac aaaaacttgt
    11581 tcaattgatc agtacaagca aaggaactgt cacagaagtc caagaaaaat taaaaacgga
    11641 atctgcgcca ttgccttatg atttaacaga gatccaaaga gaagccaatc aacgctatgg
    11701 ctattctgca aagaaaacat taagtcttgt acaaagtttg tatgagaccc ataagatcgt
    11761 tacttatccg cgtacagaca gcaaatatct gacaaatgac atgaaaagta cgatgaaaga
    11821 acgattgcaa gcagtcagcg actttgctcc tgaggtcaag cagtatttga aaaacggagc
    11881 catcgtccaa cagacaaaag tattccaaga cagtaaagtg accgatcacc atgcactttt
    11941 gccaactgaa aacagagcac gttatgaaaa attaagtaat gaagagcgaa aaatctatca
    12001 aatgattgtc tctcgttttt taggactgtt tgctcaaccc cataaagtca gccaaacaaa
    12061 agttacggtt gaatttggta aagaacgatt catttttcgg caaaatcgtg tcgtccaagc
    12121 aggatggaaa ggcgaaactg aaactgagac ggaaacagtc aaatgggaaa aaggaatgag
    12181 aatcaatccc gatttcgcca tcaaaaaaga actatctgca ccgcctaaac cattgtcaga
    12241 agcaagcttg cttggtcaaa tggaaaaata cagtctggga accccagcta cgcgtgcaga
    12301 gattatcgag aaactaatca aatctgaact gatggaacgt acccctcaag gactgcaagt
    12361 atctcctaaa ggtaagcaat tattagtatt ggtaaatccc tctttagtaa caccagattt
    12421 gacagaaagc tgggaaaaag accttgaagc tattgctgca ggaaagaaac aagcaggaac
    12481 cttcttgaat ggaatcgaaa aagaaacaaa acgcttggtg aatgagatca aatcaagcaa
    12541 acaagaatat caagatttct cgattaccca aaagaaatgc ccagaatgtg gggccaatct
    12601 gcgcgagaaa aacacacgtg acggcaaaat ctatgtctgc accaattcag attgcaacta
    12661 ccgcagaaga aaagatccaa aagtctctaa tcatcgatgc ccgcaatgtc accggaaaat
    12721 ggaaatcatc gaaggtaaaa acggtgctta tttccgttgt aaatacgatg gaacgacaga
    12781 aaaattgctt gataaaaaag agcagaagaa gaaaatgacc aaacatgaag aacgacgctt
    12841 gctgaaaaaa tatgcacaag cagaagaacc agaagaaagc ccattagcag cagcactaaa
    12901 agcagcaatg aagaagtaat aagatatgaa agaggttgtg atagaagtgg tcagcttcaa
    12961 gaaataagaa ggaatttacg aaaattgctt ctcaaatttt tgtgaaattt cggcttattt
    13021 ccgaagaagc tacttctgga acaccattta ttcggaaaaa aggactgtga caagactttt
    13081 gtcacagtcc tttttcattt aaaaaaatgt ttacttcatt tgacgtatat tattggtgag
    13141 gcttcaattc atttggcttc actttttctg tttctcttat gatataaaca ggacgctgtt
    13201 tcgtttctaa gaagattttc ccaatatatt tcccgatgat tccaagacat aacaattgga
    13261 ttccaccaat gaacaggaag atcgagacca tggaaggcca tcctgatgta ggatcaccaa
    13321 acaacaatgc ccgcaaaata atgaacaaaa ttgcaatacc agaaccgaca caagacaaag
    13381 cgccgataaa tgaagcaatc gttaacggtg catccgagaa gttcacgata ccatcaatcg
    13441 agtagttgaa taatttccag aaggaccaag atgtttcgcc agccacacgt tcgcggtttt
    13501 cataagaaat atattctgtt ttaaatccga cccagctaaa taatcctttt gaaaatcggt
    13561 tgtattctgt taattctagg acggcatcca ccatttgacg agtcatcaag cggaaatcac
    13621 gagcgccatc gaccatttct gtatcgctga ttttattgat caactggtag aattttttcg
    13681 caaagaaact gcgtatcgca ggctcgcctt cacgagtcga acgccgggtg ccgacacaat
    13741 cgatatcaga agtagtgatc cggttgatca tctcaggaag cagttcgggc ggatcttgca
    13801 gatcaacatc catcacggtc actaaatctc ctgtagaagc ttgcaatcca gcgtacagag
    13861 ccgcttcttt cccaaaattt ctggaaaatg agacaaagcg gacataagga taagctgagg
    13921 ctagcttctt gattgctgct aacgtaccgt cttttgatcc gtcgtcaata aacaaatatt
    13981 cgaactcatg ggcacctttg aatttttcaa cctcatcaaa aaaaagagga acagtttttt
    14041 cttcattaaa acatgggaca acaattgata ctttcatcgt ttctatcctt tcaatcgtat
    14101 gtttatatat ggtttacatt caaaatttaa taaaccttaa ataacacctt taaaatttga
    14161 ttttacgtaa aagaacaggt attctttttg taaactaacg tatttagttt accatgtttc
    14221 ctcggtttat caaactttta agaacaattt ttattagttg gcaagtagtt ttggatagag
    14281 aaaaaggagg aagttccagt ctattgacta ggagcaaaca aaatgaaaat acacacacct
    14341 atcggctttt tatggttaga tgcagatgaa tccggattga ctgctgtatc ttttcagcca
    14401 atttcagaaa gcgaatcagc gaatcagcga attttaacgc gcacacaaga acagtttgaa
    14461 gaatattttg cgggaaaaag aaaagaattt gatgttcctt tatctataaa gagaggtacg
    14521 ccttttcagc aaaatgtctg gaaagctttg tcgaccattc cttatggaga aatccgaacg
    14581 tataaagaag tagccgttgc tgttgatagt ccgaaagctg tgcgagcgat tgggcaggca
    14641 aatcgaacca atccattgcc aatcgttatt ccttgtcatc gagtgatcgg gcaaaatgga
    14701 aaactgacag gatatatggg aaacgcaata gatggggttg aaatcaaacg ccaattgttg
    14761 ttgttagaag gatatctgca aaattaaaaa cagttgtgat tcggacattg ttccatgcga
    14821 gaatcacaac tgttttttca tgttcattct aaactttgat cagccaaata aatagtaaag
    14881 ccatcactgt ttggagaagc ccgacagcta atccataaga taagaagcgt gtaccttctt
    14941 tcatgaattt tgcaaagtct aaacgcagac caattgccgc taaagcagtg atctcaaacc
    15001 agctgctgat aaaatgtgcc gattggtcaa taactacagg taaagaaaac aagctgttga
    15061 ccaaacaaaa gatcacaaac cctaatacat accaaggaag cggtaatttt cgttttttct
    15121 gcgtatcagt tggcgtttca aacagagctg gttgtttctt gtgcatgaat ttttcaaaca
    15181 gatagacaac aacaacgagc atcataatac gtagaatctt aaatagcatg gcgaattcga
    15241 caacagaatt gtcaagtaag cttgccccag cgactacttg accaacagat tggagtgtac
    15301 cacccaaaag tgcacttttt tcaatgagat ttccaccaaa caactgtaat ccaagaaaag
    15361 ggagagtcaa catcatcaca gtacccagta gattgactag tgtgatgatc tgtccttttt
    15421 cttcctcttt cgcatggatg gaaggggcga ttgaagcaat agcagaagaa ccacaaacag
    15481 cgtttcctcc agccatcatc aaagaagaag tatcgctgaa ttgcagtttt ttaccaatca
    15541 agtaagcacc gatgatagtg actgacattt gcagtaacac aaaaacaatt ccttttacac
    15601 caatttgagc aatcgtttga gctgtgacag ttgcacctag taaaaaaaca gagaattcta
    15661 atagtttgct ttcagcaaat tttgtgccga ttgctagaac aggctgttta aaaaaagtat
    15721 ttcctaataa aatacccaat aaaatagcaa tagtcgctcc acctaactgt ggcagataaa
    15781 tagcaagaag cctgctgaag gcagcgatgg caaaagaaag cagtaaaccg ggtaagattt
    15841 cgcggaccgc tgaatccttt acagatttta ttggatgtga caatgatttc atttgatcgg
    15901 ttcctttcgt caataaatac taaaagcata ctcgaacata gtagcactaa accagtattt
    15961 tgacaaaaga acaagaggtc gtgacaaaag tcttgtacga cctcttgttt cgaataaacg
    16021 gtgttccaga agtagcttct acggaaataa gccgaaattt cacaaaaatt tgagaagcaa
    16081 ttttcgtaaa ttccttctta tttcttgaag ctgaccactt cttttacgac ctcttggtaa
    16141 acggtgttca aaagcttgca tctgcggtaa taagcccaac cagtacaaaa acatgaacag
    16201 ctgtttttgt ttggccgttc ttattacttg atgcagaaca gctttttcac aacctattga
    16261 atcgattatt aagcgatttt tttagaaaca aattttccat gtttgctatg ttctttaatt
    16321 aagaaacgta cgtaatgagc aagaacgtag aagacaacta cacttgcaaa cagacattcc
    16381 gcaactttta agaaatccaa tacgctacta tagataccta aactgttgaa gtaatgaaca
    16441 gtagtaaata tagctgttgc agagataaca agcgggaatg taaaggctgc atagcttgga
    16501 taaaaacgaa gcttgaacaa acgtggaagc atagacagca caacgaaata caatacttga
    16561 gataaagcga acaatgctac gacaagataa aaattaggtt cagcaaagtt tttaagataa
    16621 cctgttaggc aaagagaacc aggtgctgca acgatcgtaa tcagcggacg tgtcggatga
    16681 gccatatttc ttacgaaaaa gacacgtttg atgatgatcg gcaataagaa cgcatagcag
    16741 atcaatgaaa tccaaaagaa aatttttcca ataagtggga agaagttccc ggcagttaca
    16801 gtgatgaccc caaatccaac gtaaatgata aaccagcttg gataaattgc ttcaattgaa
    16861 agatcagctt tgataacatg gtgataattg aaataaaaga caaggatcac ttggaagata
    16921 acagcgcaca gccaaatgaa tttgacaatt ggtgcaatac tttcatatga aacaaaataa
    16981 gtacaaataa ccatgagtga catcgtaaaa gttggtgaaa cggaggcgat gattggatca
    17041 tgcaacgttt gtttcgtgtg ttcgaataag ataaacaatt taacaaaaat gaatatcata
    17101 agaatcatag agataccgcc agttagattg cctaaagcaa catggtgata gtctttaaac
    17161 agatttccaa gggaagctaa cccaaggatc aacccgcaga tcgggatcgg aataattttc
    17221 aaaaagttct tcatatataa tattctcctt tttatttaat agattttagt accaagatga
    17281 acgaatacat tgtaaaatga ataggaatgt atgattacga gcaagcagac agctgattgt
    17341 cttcatctaa gttactagtt ggatcagcaa ctgttttgtt gtacatttct attatagatg
    17401 gttttttgta tatttaaatc aaatagtttt tatagtaatt atcataaaaa gttatggaag
    17461 tggtgccatg tttaagttat taacgacttt tcgagcagta tatgaaacaa gaaatttttc
    17521 cagagcagca gaagtactgt ttttatctca accagcagtc tcgaatcaaa tcaaacagtt
    17581 ggagcgggaa ttgaatatcg atctttttca acgtaacgga agaaaagaga tgcttccaac
    17641 taagcaagcc gacttattat atgaacggac actggttttg ttagaagaat gggaagaaac
    17701 tcagcaaaaa ttgctcaatg aacaaaatga agaagaagta tgtcggatcg ttgcttccca
    17761 cacctttgct gtctatcttt tgcctcagtt gatgaaacat ctgatcaaaa ggtttcctaa
    17821 agtgcatttt gagatcgaaa tcacgaattc tcatgaatca ttagaacaag tagcgaaaca
    17881 tgaatccgat tttggtttta tcgaaaagcc attagagact tccggcgtac aaagatactc
    17941 tttgatggaa gaccagctag tcatcgctgg ggacccaaaa agcaaattgt ggcttgtaag
    18001 agaagcaaac tctggtgttt ttcattacac gcagcgttat tttgaagaga tgaatatcca
    18061 agataagaag atgatcgtga aaagtaacga tgtaattgtt gcgttgctga aggaaggaat
    18121 cggtcgctcg attttgtcta agcgctctgt tccagaaaat gtgccaatga ctccgttgaa
    18181 tttccatcga tatttttatt tcatccaacg aaccaatctg gtttctgatc agctgctaga
    18241 gattgccgaa gagatccttc gatacaatga aagcacagaa acttctcaca gcgaggagaa
    18301 tctcagctgg tagataattg catgtatcaa aaaaagttct atgatacact aaagataagc
    18361 agtaggcttt tgtactgcat ttgctcaagc aactgcacaa acggaaagtt aatttttccc
    18421 aaatgtctat ggaaagactt tccattatct aactggaccg ctataataat cagcagtatg
    18481 gtttttaaga aaagggatga aacatggaaa aaaaattaaa agcttcgaac gataatcgta
    18541 ttgattatgg cgtgatttta ccagtctttc tgttatgttt gattggtctg ctctcattat
    18601 atgtagcact tagccatgat ccgaacaatc cagcagtctt gaaaggcgta gggatgcaag
    18661 ttgtctggta tttagttgga gcggcagcaa tcgttgtcat catgcatttg aattccaaat
    18721 ggatctggaa actgactcct tacctttatg gtttggggtt agtggtaatg ggactcttgc
    18781 tcaaattcta tgatcagaat ttagctgctt caacagggtc taaaaactgg tttcgattcg
    18841 gtgcgtttac tttccagcca gcagagttga tgaagattgc gtacatcttg atgatggcac
    18901 tgatcgtaac taaacataat acgcaagtaa aagaacggac gatgaaatcg gacttttggc
    18961 tgattgggaa gctcttgatc gtgacaatac cggttttagc attgatcatg gcccaagatg
    19021 actttggaac gatgcttgtc tttcttgcga tctttggcgg gattttcctg atgtctggaa
    19081 tctcttggcg catcatcgca cctgtagtaa tcttggcagt agtcgttgga gcaggaacga
    19141 tcttcttggt tacgactgaa ggcggaagag acttgcttta taaagttgga ttcaaatctt
    19201 atcagtttgc acgtattgat tcatggctgg accctttcca tgatacgtca ggaatgagtt
    19261 atcagccggc ccaaggactt ttggcaattg gtaccggtgg cttgtttggt aaaggattca
    19321 acgtaagcaa tatctatgtg cctgttcgtg agtccgatat gatttttacc gttatcggtg
    19381 aaaacttcgg ctttatcggt ggtgcattcg tcattttctt gtattttatt ttaatctatc
    19441 gtatgattcg ggtgtgtttc gatacgaata atgaatttta cgcctatatc gcaagcggtt
    19501 tgatcatgat gctgctgttc catgtctttg agaatattgg agcaaatatc ggattacttc
    19561 ctttaacagg gatcccattg cctttcatta gtcagggagg atcctcgatt ttagggaaca
    19621 tgatcggtat cgggttgata ttatcgatga gataccaaaa tgaagcacct gcaagatctt
    19681 caaggaggta agaaaattgt acacctttta ctggtacccg aaatgttcaa catgcaaaaa
    19741 agccaaagcg tggttagacc agcatggcgt tagttatcaa accgttgata tgatcaaaga
    19801 agtaccgaag gcctcaacga ttgagacttg gctagaaacg agtcaagtac caatccgtcg
    19861 cctgttcaac acaagcggga tgaaatatcg ggaacttgga ctgaaagatc aagtaggcga
    19921 gtttacggct tcaaaagcga gccaagtctt atcgacagat ggtatgctga tcaagcgacc
    19981 actgatctta cgagaggacc agcttgttgc aattggattc aatgaaaaaa tttatgaagg
    20041 agaactgata taaaatggca ggcaagcatt tgaagaaaaa ggacaacctt tggatcttat
    20101 ttaatggaaa agaatactgt gttggtttga caaatgaagc gcaagaagaa cttggagaca
    20161 tcacgtttgc caatctgcca aaagcaggac aatcgctaaa aaaagatgaa cctctgatcg
    20221 aagttgaagc ggaaaaagcg gtcaatgaat ttgccagccc gctaacgggt acagtttctt
    20281 ctgttaatga taaaatcggt gaagatatca atatcttgaa tgattcagat gaaatgaatg
    20341 cgtggatctt aagtttcaaa gatgttgatc caaaagaatt tgacgaatta taaaatcaga
    20401 aaatcacttg aaaaaagttg acactgattc ttccagttgc tataatcata agcaatccta
    20461 tatgaaacga gaaagctttg aaaagaagag tacatgtcac ggacgtttga agagagccct
    20521 gtttgctgaa aatgggtaac gaaaatgaga tggaagatgg tcttagagca gagtaagcga
    20581 gcgtaagcaa acttactacg ggagtgcccg ttacagcgcg acagtatacg gaaagaagct
    20641 aagtccaagt actggaagag gctgttattg cgaagtaacg gtaaatcaag gtggtaacac
    20701 ggaagttcaa gctttcgtcc ttttgcttaa ggcaagaggg cgaaggcttt tttgtttgaa
    20761 aaattataag agaaagatgt gaatggcatg tcattaatcc aactaacgaa tgtcaaaaag
    20821 acattcaatg ggaaaaacgg aaacatccaa gccgtcaatg acgtttcact aaacgttgaa
    20881 aaaggggata tttatggaat cgtcggctat tctggcgcag gaaaaagtac attagtccgt
    20941 ctcctgaatg gacttgagct tcccacgagc ggcgaagtga tcgtaaacga tcaagatatt
    21001 acaaaattaa aaaacaaaga attacgtaca ttccgaaaga aaatcggtat gatcttccag
    21061 catttcaacc tgttatggtc aagaactgta cttgaaaata tccagctgcc gctggaatta
    21121 gctggcgtac caaagagcaa acgtaaagaa cgggcagaag aactgttgcg tttagtcggg
    21181 ttagaaggac gcggacaagc atatccttca caactttccg gcggacagaa acaacgcgtc
    21241 ggtatcgcac gagcattggc aaatgatcct gagatcctgc tttgtgatga ggctactagc
    21301 gcattggacc cgcaaacaac agaagaagta ttagacttat tgttagcgat caataagaaa
    21361 ttgaatttga ccatcgtctt gatcacacat gaaatgaatg tgatccgcaa aatctgcaac
    21421 aaagtcgctg tgatggagtt ggggaaagtc gtagaagaag gagacgtatt gactgtcttc
    21481 agacaaccga aaaaagaagt aaccaaacgc tttgtacaac aagacatcga accagaagct
    21541 gattcgacag aactattagc tgaactcatc aaagaaaatc caagcggccg tttggcaact
    21601 ttgaccttta gcgaaagaaa tgccaacgag ccagtcattt cccaagcgat tcgacgctat
    21661 aacatcgatg tcaacgttgt gtatggaaag atcaaacaag tgaaagaagg ttcctttggt
    21721 tcattgactg tcttgatgac tggtgaaacc aaagaattag atcaagcaga agtgttcatc
    21781 aaagaacaag gagtaggaat tgaggtgatc catcgtggat aaaagctttt cagaaacata
    21841 tctagatttt aaacaaatca acccggaaac gatgaatcag gctatccttg atacactata
    21901 tatgacatta atctcaatgg tcttagtgac gatcatcgga ttagttttgg gattgatcct
    21961 ttactctatt ggacgcaaaa acaaaccgta tgctcgaatc atttatggaa tcgtatcgat
    22021 catcagtaat attttccggt caacgccatt tatgatcctg atggttttga tcattccatt
    22081 tactaaagca atcgtaggca ctatgctagg tgcgcaagca gccattccag ctcttgtttt
    22141 atccgcagca ccattttatg cccgtctagt agagattgct tttcgtgaag tcagccctgg
    22201 tgtactggaa gcagcagatg caatgggagc aagttactgg gaaatcatct ggaaggtctt
    22261 gatacctgaa agtattcccg cattgatttc tggattaaca gttacaacga tctcgatgat
    22321 cggatttact gcgatggccg gcgcgatcgg cgcaggtgga cttggaggtc ttgcttggca
    22381 ggaaggctac cagcgtggta atctaacagt gacattcgtt gcgactttaa ttattttact
    22441 aatcgtattt atcgttcaag gaatcggtga tttcttaact aagaaaacag acaaaagata
    22501 ataggatata gggggatgta ggatatgaaa aagaaaattt ttggatttgc agcagcgctt
    22561 ttgctgactg taggattagc agcatgcggt aacaacagcg acagcaaaga ttcagcaaat
    22621 aaagcagacg acacgacatt gaaagtcggc gcttcaccga caccgcacgc agagattttg
    22681 gaacatgtga aaccattatt gaaagatgaa ggaatcgatc ttgaaatcgt caaattcgat
    22741 gactatgtat taccgaacca atctttggaa gaaggcgaca ttgatgccaa ttacttccag
    22801 catattcctt atttgaacaa agaaatcaaa gaaaaaggat atgactttgt caatgccggt
    22861 gctgtccaca tcgaaccaat gggcttgtac tctaaaaaag tcaaagatgt ttccgaatta
    22921 aaagatggtg caacaatcat tacttcaaat tctgaatcag actggggacg catcatcaca
    22981 attctacaag atgctggctt agtcaaagtc aaagatggag tagatctgga aacagcaaca
    23041 ttcgatgata ttgaagaaaa ccctaagaac ttgaagttca accatacgat cgacccatca
    23101 cttttagctt ctacgtacca aaatgatgaa ggtgacctag ttgcaatcaa cgcaaacttc
    23161 gcttacggcg cagggttgaa tccattagaa gatgccgtat tgcttgaaaa ggacaactct
    23221 ccttacgtga atattattgc gactcgtaaa ggtgatgaag acagtgacaa aatcaaaaaa
    23281 ctgattgaag tgcttcaccg caaagaagtc caagaatgga tcacagacaa atggggcggc
    23341 tctgttaaac cagtaaaggc tgatgcaaaa taaggtatgg aacaaaaaag atgagaaata
    23401 agtctgtaat ggcttatttc tcatcttttt tatacctatt ctaatttaga aatcaaaatt
    23461 gtttaataat aaataaaaga tatttgattt tagtaaggaa ggtcattgcg cttcattctc
    23521 aattagtcta aaatgagtga gaacgaaaga gaatttgggg gtataatgca tgtctgtctt
    23581 agaaataaaa aatctacatg tgtcgatcga ggaaaaagaa atccttaaag gcgtaaacct
    23641 aacaatgaaa accggagaaa tccatgccat catgggacct aacggcacag gaaaatctac
    23701 cttatctgcc gctatcatgg gcaatccaaa ctatgaagta acagaaggcg aaattttgtt
    23761 tgacgggaaa aatgttttag acttggaagt agatgaacgt gcgcgacttg gcttattttt
    23821 agctatgcaa tatccaagtg agataccagg tatcacgaat gcggaattca tgcgtgcagc
    23881 gatcaatgcg aaacgtgatg aaaacgacaa aatttctgtt atggcatttt tgaaaaagtt
    23941 agatgaaaaa atggctttac taaatatgcc agaagaaatg gctgaacgtt atctgaacga
    24001 aggtttttct ggcggagaaa agaaacggaa tgagatcctg caattgttga tgttagaacc
    24061 tacatttgca attttagacg aaatcgattc aggtctggat atcgatgctt tgaaagtcgt
    24121 tgctaaagga gtaaatgaaa tgcgcggaga caatttcggt gcattgatca tcacccacta
    24181 tcaacgatta ttgaactata tcacacctga tgtcgttcac atcatgatgg aaggccgagt
    24241 agtcatgaca gggaatgctg acctagctaa acgtctggaa gcagaaggct acgctggtat
    24301 cagtaaagaa ttaggaatcg actataaaga agaagaagcg taagggggag caagaatgaa
    24361 agaaaaaaac tggctcgatt atcttgacgc ggtaaatgat ttttctttaa gtaaaggcga
    24421 acctgattgg atgagaacct tccgtcaaga cgcactagcg aaagcagatg aactgccatt
    24481 gccgcatatc gatcgggtga aattccatcg ctggtcgtta tttgatgtga aagaaacaca
    24541 aaccatcagt gaaacaggca cgattccggc atttgatgca atgaaagaca atcctgtgct
    24601 tgtccaacaa ggttcatgga cgatttttga acaactgcct gtggaattag cagaaaaagg
    24661 agtcattttt accgacttgt tcactgcaat gatcgaatat ccagaactag tccaagaata
    24721 ttatatgaaa aaagcagtaa acatgaacga agaccaattg actgcactcc atgtagcttt
    24781 tatgaacagc ggtattttcc tgtatgtgcc taaaaatgtc gtgatcgatg aaccactcga
    24841 gtccttattc attcaagatg gcgcatccga tgagcacttc ttcaagcatg tgttgatcgt
    24901 ggcagatgaa catagtgaat tttcttatct ggaacgtttc cagacaacca aagaacaagt
    24961 agcaaaatca tcaggtaata tcatcgtgga agtcatcgct aaagctggtt caaaaatcaa
    25021 atattcagcc gtagatcaat taggagaaaa tattacttct tatatgaatc gccgtgggca
    25081 cattttacga gatgcttctg tcgactgggc aattggtgtc atgaatgacg gtcatgtgat
    25141 cgctgacttt gactcagatt tagcaggaga aggtgcacac gcagaagtca aaatcgtggc
    25201 aatcagctcg ggcagacaaa tccaaggaat cgatacacga gtaaccaata aagcccctca
    25261 tacgatcggg catattttgc aacacggagt gatccgagaa aaaggaacat tgacctttaa
    25321 tggtatcggc cacatcttga aaggggcaaa aggtgcagat gcccaacaag aaagtcgtgt
    25381 cttgatgctt tctgataaag cacgtggtga tgcgaatcct attcttttga tcgatgaaaa
    25441 cgaagtaact gctggacatg ctgccagtgt aggacgagtt gatccagaag aaatgtacta
    25501 tctgatgagt agaggattgc ataaagaaga agcagagcgc ttagtgattc gaggattcct
    25561 cggttctgtc ttaacagcaa tccctgtaga acaagttcgg aaagaattag tcgaagtaat
    25621 cgaagggaag ttgaatggat gatcgatgct aaaaagctac gcaatgattt tcctatctta
    25681 tttcaagacg tcaatgatga acctttagtt tatctggata atgcagcgac gacccaaaag
    25741 ccaaaacaag tactccaggc attagcgtac tattacgaac acgataacgc caatgttcat
    25801 cgcggtgtcc atacgttagc agaacgcgcg acccatgatt atgaacaagc aagagaaaaa
    25861 gtacgggcgt tcatccatgc aaaagaaaca gcagaagtgc tgtttactcg tggtacgacg
    25921 acaagcttga attgggtagc cagaaccttc ggcgaccgct atatcaaagc aggagacgag
    25981 atagtgctct cttacatgga acatcattct aatgttatcc cgtggcagca gttggcaaaa
    26041 cgaacaggtg ctgtcttgaa atatatcgaa ctaacagaag aaggcttcct ggatatggag
    26101 gatgcagcga aaaagattac ggacaaaacc aaaatcgtct ccattgctca tgtatccaat
    26161 gtcttaggtg tcttcaatcc tgttcaagaa ttagctgaac ttgtccatga aaacggcggg
    26221 gtcatcgtag tagatggtgc tcaatctgcc ccgcacatgg ccattgatgt ccaagcacta
    26281 gactgtgatt tctttgcttt cagcggacac aaaatgtgcg gaccaacagg tattggtgtt
    26341 ttatatggta aacgaaaatg gttagaagaa atggaacctg ttgaatttgg tggcgaaatg
    26401 atcgattttg tcgaattata cgacagtaca tggaaagaac taccttggaa attcgaagca
    26461 gggacaccaa atatcgccgg cgctatcgca ttaggccatg caatcgatta tttgcaaaaa
    26521 ctcggtatgg acaatatcca tcgatatgaa gaagaactag ccgcttatgt gttgccaaaa
    26581 ttacaagcaa ttgatggatt gactgtttat ggtccacaag acccgaattt tcggacagga
    26641 gtcgttgcat tcaatttgga tgggttacat ccccatgatg tcgcgacagc tttagacatg
    26701 gaaggggtag ccgtcagagc aggtcatcat tgtgcgcaac cgctattgaa gtaccttgaa
    26761 gtatcagcga ctgctcgcgc gagcttttat ttctacaaca caacgcaaga cgccgaccgt
    26821 ttagttgaag caatcatggc aacaaaggag tttttccaac atgggactat ctaaattaga
    26881 caatttatac cgtcaagtga tcttggatca ttcttcccat ccgcatcatc gtggaagctt
    26941 gactgactcg acaagcaaga tcgaattgaa caaccccaca tgtggagatg tgatcgaatt
    27001 gcaagtcgat gtaaaagacg gaatcatcaa aaatatcgcc tttgacggca gtggctgctc
    27061 catcagtacg gcaagtgcct cgatgatgac agacgccgtc atcggaaaat caatagaaga
    27121 agcagaacat ttaactgtag actttctaca gcttgtccaa ggaaaagaag tcaaagatcc
    27181 cgaccaatta ggcgatgctg ctatgcttgg tggtgtagct aaatttcccg ctcggatcaa
    27241 atgtgcgaca cttgcctgga aagcactagg aaaagcaatg gaaaacgatg gtaccgcagc
    27301 tttccatcat taccacaagg aagaggagtg agaacatggg cgtaccagaa ttagaagaat
    27361 ataaattcgg gtttcacgat gatgtcgaac cggttttcag tacaggagaa ggcttgacag
    27421 aagaagtgat ccgtgagatg tcaagaatca aaggcgaacc agaatggatg cttgaattcc
    27481 gtctgaaatc tctagaaaca tttaacaaaa tgccaatgca aacatggggc cccgaccttt
    27541 cagacatcga tttcgatgcc atcaaatatt accaaaaacc aagtgacaaa cccgctcgtg
    27601 actgggaaga cgtaccagaa aaaatcaaag atacatttga aaagattggt ataccagaag
    27661 ctgaacgagc ttatctagcc ggtgcttccg cacaatatga atcagaagtc gtgtaccata
    27721 atatgaaaga tgaattcgaa aagttaggaa tcgtctttac tgacactgac tctgctttga
    27781 aagaataccc tgaattattc aaagagtact tttcaaaact tgtaccgcca accgacaaca
    27841 aattagcagc gctgaattca gccgtatggt caggtgggac ctttatttac gtgccaaaag
    27901 gcgtcaaagt cgatgttcca ttacaaacgt acttccggat caatgcggaa aacacggggc
    27961 aattcgaacg tacgctgatc attgttgacg aaggagcaag catccactat gtcgaaggtt
    28021 gtacggcacc tacttattca agtaacagcc tgcatgccgc catcgtagaa atctttacaa
    28081 gaaaagatgc gtattgccgt tacacaacga tccaaaactg gtcagacaat gtgtacaact
    28141 tagtaaccaa acgagcaaaa gcatacgaag gcgcaaccgt tgaatggatc gacggaaacc
    28201 taggtgcaaa aacaacgatg aaatacccaa gtgtttactt agatggaaaa ggtgcacgag
    28261 gcaccatgct ctccatcgcc tttgcaggag caaaccaaat ccaagatacg ggtgcaaaaa
    28321 tgatccacaa tgcaccaaat acatctagct ctatcgtctc taaatcaatt tcaaaagacg
    28381 gcggagaagt aaactatcga ggacaagtaa cctttgccaa aaacagtgcg ggttcgatct
    28441 ctcatatcga atgtgacacg atcatcatgg acgaatggtc gaaatccgat acgatcccat
    28501 tcaatgagat ccacaacagc caagtctctt tagaacacga agccaaagtc tcaaaaatct
    28561 cagaagaaca actctactat ctgatgagcc gaggcctgac cgaatctgaa gcaactgaaa
    28621 tgatcgtcat gggctttgtt gaaccattca ccaaagaact tccaatggaa tatgcagtcg
    28681 aactcaatcg attgatcagt tatgagatgg aagggtctgt aggttaataa ctatttcatt
    28741 gctcgaaacg ttgatattaa agcgtttcga gcgttttttt atttgtattt taatggttgg
    28801 ctacgttttt ggctacgttt ggaattattc gcccataaat tttgcaaatc ggtcaccagt
    28861 tttcttaaca gattgtggtg tgacatgagc atagatattc atggtagttt taatatcttt
    28921 atgacctaat cgttcctgta cttcttttat cgacgcacca ctttcaaaca ataagctaca
    28981 gtgggtatgc ctaaatccat gtggagtaat ttgaggctcc atcggtgtct ttttgtaaat
    29041 ccagtctaac caatcgttta ctacttgggg gtagtacaac ttgtttgtgt ttgtagtgaa
    29101 aagaaactgt tttggtgaat tcgtgttata gccatataag atcattattt tcatttgttc
    29161 ttgttgccaa atacgcagtt gtttaagtgt aaaatcatca agctgaatcg ttcttgatga
    29221 atttttagtt ttaggtactt gtagaactac ttgattaaac tcgtcttgag cgatagtttt
    29281 accaattgtt attgttttat tgaaatagtc tatatcttcc cattgaagcg ccaatgcttc
    29341 gcttttacgc attcctgtat aagctaataa acggaaaaaa gtgtacagtt taactgaatt
    29401 attttcttta gtattctgta aaaacagttg taattgatct ttcgtgtaaa acttcaaggg
    29461 ttgttcttct tcaatttttc ttggaagcaa tgttttagac ataggattat ccttacagac
    29521 ttccattgct actccaaatt taaatatttg agcagttgtt tttctcaaga aaccatattg
    29581 ttttaatgtt ttgtgccatt cattcacctg tttttggcaa tagctaacac ttattttatc
    29641 caatcgtaat tccccaaaag caggtaatat atggtttttg caaaaacgtt gagcaacggc
    29701 aacagaagac ggttttactc ttgttctgta gttatcaatc cataattcat acaactcttg
    29761 aaaagttgta acattgattt taaactcttg tttttcttta aagtctacaa gtagtctatt
    29821 aagtgctgtt tgcgcctcag cttttgattt gaatcttcta cgctttgtac gtatatcttt
    29881 tccagtcttg ggatctttcc ctaaataagt ttcaaatccc caatattttt cgccattttg
    29941 taatttataa ctaaaaaatg ttgccatttg attctctcct tacttcatta ttgcaggtag
    30001 agaagttatt tgaagaatca attattttta aatttatcgt gattatcagt taaaaatttt
    30061 gcgattatac tatttttctc atatgggaaa ttgtattttt tactaagatc tgaaataaat
    30121 ttttctgttt caaattggtt tttagtaatt ttataaaaga aatcctcgac agatgaatac
    30181 tttgaaatat ctttataggc taagtccatt tcgtctaata gctgttttaa agaatcaatc
    30241 attctttctt tttcaccgta tttgaaatca ccatttctta tcttgtcttg aagtttactg
    30301 ttggctcttt tgaaaaatag ctctttagag atagagtgtc tttggttagc gtactcttca
    30361 atcagaggat tgatattatt agcaagtatc tcatttagat aaatttcaaa atcttttgag
    30421 taatattttg taaatatatt ttttattata ggattcaata actcatagtc tttatctagc
    30481 gaaaacgtat cactatatct ttcttgaatg tctttaaaga ctttgtgagg tatttctggg
    30541 aagtccttaa tatatagctc ataccctata tcgataaggt atgaagttat atattcttct
    30601 aaagttcccc atttaatcca gtcaattgtt gaattagtga tagttgataa taatttcagc
    30661 ttttgtggat ttggtaaatt ggtcccacgt tcccagttgg atatggtcgc acgtggtgaa
    30721 ttatctattt tccttccaag ttcttccata gtaaaaccac atgaaaggcg taattgtttg
    30781 attcgttttc ctgcttctga aggatttggc ttcatctttt ttctcctttt tatt
//
LOCUS       DACCXO010000023        30816 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.024, whole genome shotgun sequence.
ACCESSION   DACCXO010000023 DACCXO010000000
VERSION     DACCXO010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.024"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            621..1949
                     /locus_tag="IVY70_000982"
     CDS             621..1949
                     /locus_tag="IVY70_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP7958126.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2151..2984
                     /locus_tag="IVY70_000983"
     CDS             2151..2984
                     /locus_tag="IVY70_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP7958127.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3000..3737
                     /locus_tag="IVY70_000984"
     CDS             3000..3737
                     /locus_tag="IVY70_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP7958128.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3892..4860)
                     /locus_tag="IVY70_000985"
     CDS             complement(3892..4860)
                     /locus_tag="IVY70_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP7958129.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5092..5925
                     /locus_tag="IVY70_000986"
     CDS             5092..5925
                     /locus_tag="IVY70_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP7958130.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVFKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5959..6798
                     /locus_tag="IVY70_000987"
     CDS             5959..6798
                     /locus_tag="IVY70_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7958131.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6824..7384
                     /locus_tag="IVY70_000988"
     CDS             6824..7384
                     /locus_tag="IVY70_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP7958132.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7583..9304
                     /locus_tag="IVY70_000989"
     CDS             7583..9304
                     /locus_tag="IVY70_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP7958133.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9469..10611
                     /locus_tag="IVY70_000990"
     CDS             9469..10611
                     /locus_tag="IVY70_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP7958134.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10627..11838
                     /gene="thiI"
                     /locus_tag="IVY70_000991"
     CDS             10627..11838
                     /gene="thiI"
                     /locus_tag="IVY70_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP7958135.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12411..13058
                     /locus_tag="IVY70_000992"
     CDS             12411..13058
                     /locus_tag="IVY70_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP7958136.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            13451..16096
                     /locus_tag="IVY70_000993"
     CDS             13451..16096
                     /locus_tag="IVY70_000993"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP7958137.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16406..17719
                     /locus_tag="IVY70_000994"
     CDS             16406..17719
                     /locus_tag="IVY70_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP7958138.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17709..18362
                     /locus_tag="IVY70_000995"
     CDS             17709..18362
                     /locus_tag="IVY70_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7958139.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18417..19109
                     /locus_tag="IVY70_000996"
     CDS             18417..19109
                     /locus_tag="IVY70_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP7958140.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            19259..19459
                     /locus_tag="IVY70_000997"
     CDS             19259..19459
                     /locus_tag="IVY70_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7958141.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(19713..21392)
                     /locus_tag="IVY70_000998"
     CDS             complement(19713..21392)
                     /locus_tag="IVY70_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP7958142.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(21394..21606)
                     /locus_tag="IVY70_000999"
     CDS             complement(21394..21606)
                     /locus_tag="IVY70_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP7958143.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            21999..23729
                     /gene="efrA"
                     /locus_tag="IVY70_001000"
     CDS             21999..23729
                     /gene="efrA"
                     /locus_tag="IVY70_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP7958144.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            23726..25486
                     /gene="efrB"
                     /locus_tag="IVY70_001001"
     CDS             23726..25486
                     /gene="efrB"
                     /locus_tag="IVY70_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP7958145.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            25581..25778
                     /locus_tag="IVY70_001002"
     CDS             25581..25778
                     /locus_tag="IVY70_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958146.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            25931..26416
                     /locus_tag="IVY70_001003"
     CDS             25931..26416
                     /locus_tag="IVY70_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP7958147.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(26520..27113)
                     /gene="clpP"
                     /locus_tag="IVY70_001004"
     CDS             complement(26520..27113)
                     /gene="clpP"
                     /locus_tag="IVY70_001004"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP7958148.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            27293..27820
                     /locus_tag="IVY70_001005"
     CDS             27293..27820
                     /locus_tag="IVY70_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP7958149.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            27911..28648
                     /locus_tag="IVY70_001006"
     CDS             27911..28648
                     /locus_tag="IVY70_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP7958150.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            28652..29569
                     /gene="miaA"
                     /locus_tag="IVY70_001007"
     CDS             28652..29569
                     /gene="miaA"
                     /locus_tag="IVY70_001007"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP7958151.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            29571..30800
                     /gene="hflX"
                     /locus_tag="IVY70_001008"
     CDS             29571..30800
                     /gene="hflX"
                     /locus_tag="IVY70_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP7958152.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10176 a         5272 c         6575 g         8793 t
ORIGIN      
        1 taggttcgag tcctactgcc ggagtaaata aagcttatgc aaagaaaaag aaatccaatc
       61 acttgggttt ctttttcttt tattattatg atgaatcaag ctttctgttt ttgtcacgta
      121 tgttgtaatc tttacgaaag agtagagttg gctactcata atcaaccata cttcccatca
      181 tcgctaaata cgaaataaat cagcattcct acaccaatca aagcacaagc tgcaccaaga
      241 aaagggatga cattcccaaa gttatctgta ttcataagta tgccaccacc cacaaacaaa
      301 aagccgccag caatcaggtt tttcatttta ttcatctcct ttcttacgtt atagtatttc
      361 cttctttcaa tgagtatttt gaaagctatc ttattgtatg atgttcttct aggcctcttc
      421 tttattatac aggccatttt cgttatctca tagaaatatt cctttttatc tgaaaactat
      481 tgtataccgt atgacatttt tcttcatgaa gttgtaagta tattctcatt caaatgaaaa
      541 tttcatcata gaagagattg tatttctaaa ttcttaagag taaaatacga agtataactt
      601 ggatttggaa ggatgattgg atgaaagcta ttacgcctga tatgacaaca aaatttaaca
      661 aacagtttca agcaaatatc aaacagcagg cattgcagcg ggcagttgtg aagaacggaa
      721 ttactgcttc tgctgaaaat gtttcagcac atgtggagaa tacaccggta ttttctatag
      781 atttggctac ggggaaagtg gctaatcaaa tgcaatcggg gcgttgctgg atgtttgctg
      841 cactgaacac attccgtcat aagatgctga atcaattcca tttaaaagag tttgaattat
      901 cacaaaatta cacttttttc tgggataaat atgaaaaggc aaattatttc tatgaaaaca
      961 tcattgcaac aggaaaagag gcaatcgata gccggaaagt agctttctta ctcgctactc
     1021 ctcaacaaga tggcggccaa tgggatatga tcgtttcatt attccaaaaa tatggcgttg
     1081 tgcctaaatc ggtgatgccg gaaagtgcga atagttcaaa ttctcgagat ttgaataact
     1141 acttgaacaa attactgcgt aaagatgcag tagtgttacg gaaaatggtt gctcaaggag
     1201 aaacattaga cgctattgaa gaaaagaaag aagaaatgct agaaagcatt tataacttct
     1261 tagctatctc attaggaaca cctccaaaag aattcgattt tgagtaccgc gatgaagaaa
     1321 agaactatca tcttgaccga ggactgacac cacaaatttt ttatgataaa tacatcggtg
     1381 taaaattaga tgattatgtt agcgtgatca atgcaccaac aaaagacaaa ccattcaatc
     1441 gttcttatac agtagagatg ctaggaaatg tcgttggcgg gaaagaagtc aaatatctga
     1501 atgttgatat ggaaactttc aaaaaattag cgattgctca gcttgaagaa ggagaatctg
     1561 tttggtttgg ttgtgatgta ggtcaatctt ctactagaac tagtggcatc atggccttag
     1621 atgcttattc aatggatgat ctatttgata cggattttac aatgactaaa gctgaacgtt
     1681 tagattttgg agaaagtctg atgacccatg cgatggtatt gaccggtgtt gacttagtag
     1741 acgggcaatc aacaaaatgg aaagtcgaaa atagctgggg cgaaaaagtt ggaaagaatg
     1801 gcttctttgt tatgagtgat gcctggatgg acgaatatac gtaccaaatc gttgtaagaa
     1861 aagatttatt gacgaaagag caattaaatg cgttcaatga agaaccaatt gttctatctc
     1921 cgtgggatcc aatgggtgct ttagcataga cgagtatttc aatgaagagt atgtagtaaa
     1981 gataaaaaga gctggcttaa gccagctctt tttatcttta aagtccattc cctgtttttt
     2041 atggtccctg tgctataatt ctaaataaag gataaggatt ggaaataatc cttttatcat
     2101 taaaaatatg atgacgaagt agaataaaat tgggagtagg ggggaaagtc atggatatcc
     2161 aaactgtttt atctgatctg aaattaaatg gtaaagtgat tccagttgtt ggtggcgatg
     2221 ttaatcagac ctatcgtata aaaaccgaac atcgtgctta ttttttaaaa atccatccga
     2281 atgtaaaaaa aggatttttt gaagcagaag tagacggatt aaaagaactg tcagcattcg
     2341 tacgtgtacc ggatacgtat atgctaggtg aaacaagtga aggcgcttat ttgttaatgg
     2401 aatggatcga gccgggaaaa ggggatcaac gagatcttgc tgcagcttta gctaatctcc
     2461 atcagcaaac agcgccacaa tttggttttc gtaaagataa ctatttgggt acacttgttc
     2521 agaaaaattc ttttgaagaa gattggtgga cttttttctt taaagatcgg cttgaatccc
     2581 agatttcttt ggcagaagaa acgaatcgat ggaatgtaca gcgtcaagaa aagtatctgc
     2641 gatttaaaga acgtgtattg aaatcggttg aaccaaaaaa gatcactcca cgcctcttgc
     2701 acggagacct gtggagtggt aatgtgttct ttgaccagca agggcatcca gtttttgtcg
     2761 acccagctgt ttcttatggg aatcgggaac aagatattgc aatgagccag ttattcggtg
     2821 gtttccgacc ggaattttta gatgcttatc aaactatatt tccattagaa aagggatgga
     2881 aagaccgact gccgatttac cagttatatt atcttttggc acatttgaat atgtttggag
     2941 aaagttacgg ttcccaagta gatcaattac tagaaaattt ttaagaggag ggcgtgtttt
     3001 tggcaaaaaa atataccaaa aagcatgaag tttcatatta tgagtgtgac atcaaccaaa
     3061 cgatgacatt tccttcgatg ttaggagtag ttatcaaaac gtctgaagat caaagcgacc
     3121 agttgggaag aggcagtgaa ttcgtcaata gctttggact tacttgggtc atcacgaatt
     3181 attccatgga gattaatcgt ctccctagag tcggtgaagt aatctcagta acgactcaag
     3241 ccatggagta caataagttt ttctgttatc ggaacttctg gatccatgat gaagatggga
     3301 aagaattagt caaaatagaa tctgttttcg tattgatgga ttttgtgaat cgaaaaatga
     3361 gcagtgttaa tgaagagatc attgctcctt ttgagagtga gaagatcaag aaaatcaagc
     3421 gtcaggaaaa aattgaaaag attgattcgg gcttcatgtt gccatatcgt gttcgttttt
     3481 atgatatcga tagtaatcag catgttaata atgcgatgta ttttaattgg atcattgacg
     3541 tattaggata tgactttttg actacgcatg ttccagaaaa agtaattatt cgatttgata
     3601 aagaagtaga gtacgggaat gagatcgaaa gccattttga acaaatccct atcgaaaatg
     3661 gtgtcaaaac aaaacatgaa atccgaatgc aagaacagct gtattgtgaa gcaaatatta
     3721 tatggaaaaa taaataaaaa agaggttgtg acagaagggg gcaacttcaa gaaataagaa
     3781 gaaatttacg aaaattgttt ctcaaatttt tgtaaatttt cgacttagtt ttcgaagaag
     3841 ctatttttgt cacagcctct ctttttaagt ttagctatta gataatgatg tttagttttg
     3901 gataaacgaa atcaattctt cacccgacaa accatagttt acagcaatca atagttttaa
     3961 acgtgctttt tgactattaa tactgttaca gaaaataatc cccatttttt ctagttcttt
     4021 tcccccgcct tcataatcat aaacaggctc tgcgattcca ttgaaacaac gagaaacaag
     4081 tactacaggg attttttgga caagaagctt ttccaaagca gctaaggctt gtggagggag
     4141 attccccgca cctaaagctt cgattaccaa accgtctact ttcgtatggg cgatggcttc
     4201 caataagtcc ccttgcatcc cagcatatgc tttaacgatc gggatagtcc catctacagc
     4261 ggaaatgtcc agtcgctttg tttctaaaag ttcctgcaag aataaaattc gattcttagt
     4321 tactaagccg atgggaccca atgtaggtgt tcggaaagta gcaacatttg tggtatgggt
     4381 ctttgtcacg tagcgtgcag aatggatctc atcgttcatg accaccaaaa cccccttatc
     4441 tagcgcttct tcacaacttg cgacacgaat cgcactttca aaattataaa ggccgtcact
     4501 tccaagttca ttacttgaac gcattgcccc agtcaaaaca attggtaatt tgttgccaat
     4561 cgttgtatca agaaaaaaag cagtttcttc taatgtatct gtaccgtgtg tgatcaccac
     4621 tccggatata cctgatgcat aagcggacaa gatacgttct tttaatacca acatgtgaga
     4681 aggcatgatg tgtggtgagg gaagattgaa gatatcttca actaataatt ctacatgctt
     4741 ggggattgtg atttctgcat ctaataatgg gtttgccaca tcaggactaa caccgcccgt
     4801 gagcatgttt tctttcatgg cgatcgttcc gccggtgtgt agtacaagta tttttttcat
     4861 tttacagact tccttacgaa taattttgca tagccgttaa atcttattgt atactatata
     4921 aaagagtttt gctatcagtg aagaataacg aatagcctaa cttatcataa aaaagctgac
     4981 cttattaaaa tggaaaagac tcaaccttga cctcctaata caatggagta tctggttgag
     5041 tttcttttac aagaaagcag ccaaggaaat caatcacgaa ggtggccgga aatgatcaga
     5101 atcggattaa catcgtttaa agaacatgac aaattaactg gtaaaaagaa taacactctt
     5161 tatgaatatg cagggtttct acccttagtg gaattagata ctgcctatta tggaattccc
     5221 cgaaagtcct cagttgagaa ttggataagt gaagtacctg aatcatttcg ctttgtattc
     5281 aaagtctatc aaggaatcag cggtcaagga gactggaagc cttattatca gaccgaagaa
     5341 gagatgattg cgcatttttt agatgttatg tcgccaatgg tagatgctgg aaagatgttt
     5401 tgctttttag tccaattccc ctcatcattc aaatgtacga aagaaaatgt ccagtacttg
     5461 cgcaaactta gaaagtggtt tgagggttat cctgtttcta tagaattgcg ggattattct
     5521 tggtacagcg atgaatttct tgaacgtaca agaaacttta tgtctagagc cgctttttct
     5581 ttagccatca tcgatgagcc acagttgctg aatacaacga ttccttttga tccatttgtc
     5641 accaatccga actttgctct ttttcgattc catggtagga atgcgcaagg atggcaagca
     5701 aatgataaag agtggcgtaa aaaaagaacg ttatatcgtt attcagaaaa agaactgcaa
     5761 gaactagcac atgaagtcaa agtagttgat tctaagacaa aagaggtagg tgtgattttt
     5821 aataataatt ctggtggtga tgcggctgaa aatgcattag cactgcaaaa aatattaaat
     5881 atcaagccag atggactcaa tccaacccaa atggatctgt tttaagggct tattttattc
     5941 aagaaagggg cgaagtctat gatcaaagct attttttttg atattgacgg aacattggtc
     6001 accaatcgtt caaaagcgtt ggaatcaaca aaacaagcaa ttgaatatgc tcggaaaaac
     6061 ggcattttat gcggtgtagc aacggggcgc agcccagtaa agatcaaaga aatcattgat
     6121 gaattggaat tagatatgta tgtcgtgtat aatggtcaac tggtttttac tgcagatcgc
     6181 acgattatcg accacccttt tgaacaaaaa gtattggaac atattgttga atttgcagat
     6241 gagaaccatc gacaaatcgt ttttggtgcg agaaatcggt tagatggatc aacgacgatg
     6301 ctattaggac aatcgatttt tatcaagcga ctagtcagct ttttaccgcg taaattcccg
     6361 gtacgtctga tgaaaaaaat cctccaggtt tttagccctc accgacaaaa ggatcgttat
     6421 gaaacgttag agatattaaa agaaccaatt tatcaatgta tattgctcag tccggagtca
     6481 gaacagaaaa agttagaaca tcgctttccc gagtgtacgt ttcagcgttc aaatacgtat
     6541 actgtagata tcatcccaaa aggtggttca aaattactag gcatccaagc tttcgcaaat
     6601 gctgtcgaaa ttgaaatgga agaaattatg gcattcggcg atcattacaa tgatatcgag
     6661 atgctgaaag gtgtaggaat tggagtggcc atggggaatg ctcagatcga agtaaaacat
     6721 gctgcagact ttgtgactca atcaaatgaa gaagatggga tatacgaagg actgaaacac
     6781 ttcggtctga tttattagtc aatgaaagaa ggaaaaaatc agtatggaag cagagacacc
     6841 ttttcaaaaa gtagaaacat tccacaaaac ttttgatcca agaagaccag agatacccac
     6901 ggcttttaca gaaaatgaag ctcatacacg tgctgcgttc aaaatagaag aacttgttga
     6961 atttgttgca gctacttcac ctaataaaga acaactgcat gaagctgtta tgctgctgca
     7021 cgaagcatta gataaagcag aagaaaaaat acttggtaaa acacagtggg gaagtgatcc
     7081 gctggttgaa caagtagatg cactgacaga tatcctttac tttacttatg gttctttttc
     7141 attactagga atagtgcctg atcgaatatt cgaaatcgtc catcaggcaa atatggggaa
     7201 attatttcca gatggaaaac ctcattatga ccctgtaaca aataaagtgt tgaaacctga
     7261 aaactggcag atggaatatg ctccagaacc taaaatcgcg gcagaaatcc aacgtcagat
     7321 tcaagaagcg attgctaaga agaaatccgc tgatataggc atgtgcacaa atgaaaaaga
     7381 gtgatcagag tttcactatt tttgttatct ttatacgatg gaaaaacaaa agattttcgt
     7441 ttaagttagt gaatgcatga aaatagtatg ctataatcta gaatagatgt cttttgcacg
     7501 agagacgtat tatttggatt tatgccaagc agaacgccat ctgttgcata aaagatgcat
     7561 actgaaaacg gaggaaccat aaatgggaaa taatgttatc atcggaatcg tcattgcaat
     7621 cattatagtc gccattgtcg tctttttggt tagttacttg atgaagaaaa aaaaccaaga
     7681 tcgattggat gccttagaaa aacgcaagga agaattattt gacttgccag tgattgaaga
     7741 agtcgatgat gttaaaaaaa tgcatctggt tgggcaaagc caaaatacct ttcgtgaatg
     7801 gagtcaaaaa tggaatgaca tatctacttc ctcgttcgct gaactagaaa gtcaaatttt
     7861 cgaagtggaa aacctcaatg aaagttttcg gttcttaaaa ggaaaaaaag cagttgttga
     7921 agcagaagct acaatggacc aaatggagaa acaagtgacg gaaatccgtg aaggtctaaa
     7981 ggaattgcgg gaaacggaag aacgcaactc actagctgtt cagcaggcac ttgacgtcta
     8041 tgaagaactg aaagaaaaag tcagagaaga cgagcatgaa tacggaccag ctctttttga
     8101 attgcaaaaa cagatcaaag tgatcgaaaa agaatttacg cagtttgtga ctttaaacac
     8161 ttctggagac cctgttgaag cccgcgaggt tttggataaa gcagaaaaac atacgtatga
     8221 agtggaagct ctgatgaaaa aaattccacc gctgtatgag gagctccata ccactttccc
     8281 tgaacaactt gaagaaatca gtgatactta cgagaaattg atggaagaac aatatgtatt
     8341 cccagaagaa gatcttgctg aagatatcgc aaaagtaagt cgaagaattg aaaattcatt
     8401 agccaatctt gaaaaaacag aagttgaaac ggtcgaattt gaaaaccgtg aaacagctga
     8461 tttgattgat tcgttatatg atatcttaga acgggaaatg gaagctcaga gatatgtaaa
     8521 aactaaccaa tcaacgattg cagagtatat caaacataca acaaagaata accgtcaatt
     8581 attgattgag ctggatcaca catctcaaag ctatacattg aaccacaatg aaattggccg
     8641 tgtacgaggt ttccaaaccg aagtggaaga aatggaacgt caaaatgaac agatgattcc
     8701 acagatccgt caacacgaaa ttccttattc agaagtgcgc acgttctata aaacagtctt
     8761 caaagtctta gaagatatcg aaaagcaaca agtagagatc gacgactcat tacatgaatt
     8821 gagaaaaggc gaaaaagaag cgcaagaaaa gatcgataca ttcgaattca aattgcgcag
     8881 tttgaaacgt tttgtagaaa aacaacgttt gcctggcttg ccgaatgatt acctagaatt
     8941 cttttttgtt gctactgacc gtattgaaga actaagtgtt gtcttgaata aaattcgagt
     9001 aaacatggaa gaggtcaatc gtttagttgc tctatgtgaa gaagatctag aattattaga
     9061 taagaagaca catgatttag tagatgcagc agcattaacg gagcaaatgc tccaatatgc
     9121 aaatcgttat cgtcatacac acgaagaagt gagagaagca attgataaaa gctactattt
     9181 attcaataaa gaatatcact atcaagaagc attagacgaa atcggtacag cattagagcg
     9241 agtagaacct ggtgctttca aacgcatcga agatttttat tttaatcatc cagacttagt
     9301 ataaagcaaa aaagttagta aaccagcaag agcaagacgg attattgtct tgttcttgtc
     9361 tttttttgta tatttgaaaa ttgaaacaag tgaaaatttc acctgctctt gaaagcacct
     9421 tggacaaccg ttataataga aaagaatttc aaaaaagagg ggtttcttat gatatatttt
     9481 gataacagtg caacaacccc aatctttccg caaagcttag atgcatatgt aaaaaccagt
     9541 cagcggatta ttgggaatcc atcaagcttg catgatttag gcaatcaagc taaccggttg
     9601 ttgcagcaag caagaaagca gatcgctgat ttgatccatg tagaaccaga agaaatctat
     9661 tttacttcag gcggaacaga aggagataac tgggtattaa aaggaacgat gatcgaaaaa
     9721 agagaatatg ggaatcatat gatcatttca ggagtcgaac atccggccgt cagcgagaca
     9781 gcagaacaat taaaagaatt aggaatcgaa gtaagtatcg ccccagtgga taaacgcgga
     9841 tttgtacgtg tcgacgaatt gaaagaatta ataagaaaag agacagtatt agtctctgtt
     9901 atggctgtga ataatgaagt aggatcgatt cagcccatcc aggaaatcag tgatcttttg
     9961 gatgattttc ctaaaattca tttccatgta gatgctgttc aggcgattgg aaaagtacca
    10021 ttctcgcaat ggttgacaga aagagttgat tttgcaacat tttcagctca taaattccat
    10081 ggcccacggg gaactggttt catttattgg aaaaagggtc gtcgtttagc accattactg
    10141 acaggtggag ggcaagaaag aaatcagcga agtgggacag aaaatgttcc aggtattgtg
    10201 gctatggtta aagctttacg tttatcatta gagataaagg aacaaaaacc tgagcagaca
    10261 caacgcttaa aagtagctct aatcaatgcg ttgaagacgt atgagaaagt gacgattttt
    10321 tcagaaggaa ctgactatgc accgcatatt ttatgttttg ctctaaaagg aattcgcggc
    10381 gaagttctcg tccatgcctt agaagaaaaa caaatatttg tttctactac tagtgcctgc
    10441 tcctcacgta aacatatggc aagcagtaca ttgcacgcaa tgcatgtacc aaatgatctg
    10501 gctacatctg ctgttcgaat cagcttagat gaaaacaatt cgcttgcaga agtcgagcaa
    10561 tttatgatta ttttcaagca gctttataag aaattttctg caatcaatta aaagaggagt
    10621 gttcttgtga aatacagtga aattatggtc cgttatgggg aactttcgac aaaaggaaag
    10681 aatcgaaaaa gctttatcat gcaactagca caaaatgtca gacaagcttt gaaagacttt
    10741 ccagaaatca aaatccatgc tgatcgggac cggatgcatc ttttattgaa tggcgcagac
    10801 gggcagctag ttattccaaa actagcaaaa atatttggga tccaaaattt ctctccaagt
    10861 attcgtgtag aaaaagatgt cgaagttttg aaacaagctg ttcaagacat catgaaagaa
    10921 atttatactg ggaaagaaac atttaaaatt atcgctaaaa gaagcgatca ccaatttgaa
    10981 ttgacaagta atgaattgaa tcaaacatta ggtaatgctg tttttgatat tttcccgcat
    11041 atccaagttc aaatgcgtca accagatatt ccattacgtg tagagattcg gcgtgatggc
    11101 gcttatctat cgtatgaaac gatcaaggga gctggaggat tgcccgtagg aacaagcgga
    11161 aaaggaatgc ttatgctttc tggggggatt gattctcctg tagcagggta ctttgccatg
    11221 aagcggggag tggaaattga agctgttcat tttgctagtc cgccgtatac aagtgaacaa
    11281 gcactgcaaa aagcaaaaga tctaacagcc aaactagcac cttatgtggg aacgatccat
    11341 tttatcgaag tgccatttac cgagattcaa gaagaaatca aaaagtcagt gccacaagga
    11401 tattggatga caattactcg tcgaatgatg ttgcgtttga ccgatgaaat tcgccgtatt
    11461 cgtcatggtt tagtcatttt gaatggtgaa tcattaggtc aagtggcttc acaaactttg
    11521 caaagtatgg tggctatcaa tgaagtaacg aatacgccaa tcattcggcc tgtagcgaca
    11581 atggataaac ttgaaatcat tgaagtagca gaacagatcg atacatttaa tctttctatc
    11641 cagccttttg aagattgctg tacgattttt gctcctccac agccaaaaac acgccctcaa
    11701 ctggaaaagg tccatcaata tgaagagcgt ttagcaatag atgaaatgat ccaacgagca
    11761 ctagctggct tgaagatgga agagatacaa gcagaatcgt ctaatgaaaa aactgaagaa
    11821 ttttcagaat tgctataaga ggttatgaaa atagtgtttt gaccagagta ttagagaaac
    11881 aaaacgaaaa aatagctcca tgtattttac cgtttttgaa ctaatgtcga agattagctg
    11941 attgaatacc atttatcaag aagttatgga aaagccggtt cttttttact aactgaaaaa
    12001 taaaatttta tttagagatc aagctacagt tgataaatga tataaaaaag agaatttccc
    12061 atgaaatagc gaggttgtga cagaagtggg cagcttcaag aaataagaag gaatttacga
    12121 aaattgcttt tcaaattttt gtgaaatttc ggcttatttt tcgaagaagt tacttctgga
    12181 acaccgtgta tcagaggttg cgaaaaagct gccctgcatc aagtaataag aacagccaaa
    12241 caaaaatagt tcctcatatt ttacgtttgg ttgagcttat taccgcagat gcaagctttt
    12301 gaacaccgtt ttatttgtct cacacccttt tccttcctac ttgtaacctt gctcccgtgt
    12361 tagaaacatg ttacactaaa tatgtgaaat tattaacaag gagcgtcgtt atgcgagaaa
    12421 aaacagatac actgccaaaa gcaactgcga aaagattacc tttatattta cgctacttga
    12481 agatgctgga tgactcaggt ataagtagaa tcaaatcaaa tgagttcagc gaaatcaccc
    12541 aaattccttc tgcaacgatc cgcagagact tctcgcaatt aggcgagcta ggcagaagcg
    12601 gatatggcta tgatgtccct tttcttatcg atgtattcaa taatatcctt aatactaaag
    12661 aagaaaaacg aatcgcatta gtaggatatg gaaatcttgg caaagcattg aaacacaaca
    12721 attttagaag aaatgaaaat ctaaatatcg tgtgcgtctt tgataatgat ccagcactga
    12781 tcaatcgagt aattgatgga gaaatgatct atccgattga tcgtttcgcg gagatagcca
    12841 aagcaaaaaa tgtgacagta gcaatctcta cggtaccaag taaatattca caatctgcta
    12901 ttgatgaaat tgtcaaagga aatgtaaccg ctatacttaa ttttgcgcct gacagagtaa
    12961 ctgtcccagc ttatgtcaat gttcaataca ttgatttgac aaccgagttg cagacgctta
    13021 tttactttga tgagaattac tcagaagttt tttcttagga aaatttgagt aggcttattg
    13081 atcttgcgag aactcgtcgt aattctttca aaaatttttt agtactaaat gaagtggttt
    13141 tttcacgacc acttgttgac taaaaaaaaa aaccattgta gaatgaagca taacgctgaa
    13201 caagaggagt aactggcaca ttcattctta gagagagaac tatctgctga aaggttctca
    13261 atgacaacca gtgaaggtag cttgggagtt tctgtcttga atggagtaga gatagacgag
    13321 tcgtgatcgt taactcactt gagaggcaat aatgattgat tattgcaatt taggtggtac
    13381 cgcgtaaatc atacgtccta gaatacgaaa gtattttagg acgtttttta gtttaaagga
    13441 ggaaaacagc atgacggaag aaaataacct atcaacaaaa tataatccgc aagaagttga
    13501 agctggacgt tatcagcatt ggttagaaca agatttgttt aaaccgagcg gagataaaaa
    13561 agcaaaacct tattcaatcg taatcccgcc cccaaatgtt actggaaaac ttcatttagg
    13621 gcatgcttgg gatacgacct tgcaagatat gctgattcgt caaaagagaa tgcaaggata
    13681 tgatacttta tggctgcctg gtatggacca tgcaggaatc gcgacacaag caaaagtaga
    13741 agcgaagctt agagagcaag ggatttctcg ttatgatcta ggtcgtgaaa agttcatcga
    13801 tcaagtgtgg gaatggaaag aagagtatgc gagccacatc cgcgaacagt gggctaaaat
    13861 gggcttatct ttagattaca gtagggaacg ttttacatta gatgatggat tatctgaagc
    13921 agtacgtaaa gtttttgtta ccttatacga aaaaggtttg atttatcgtg gtgaatatat
    13981 catcaactgg gatcctcaag cacgaacagc tctatctgat atcgaagtta ttcataaaga
    14041 tatcgaaggc gccttttatc atatgagcta tgaactagca gatggatcag gcgtagtaga
    14101 aattgcgaca acccgtccag aaacgatgtt gggagatacg gcgattgctg ttcatccaga
    14161 agacgagcgt tatcaagcgc tcatcggcaa aaaagtgatt ctacctttag tcaataaaca
    14221 aatcccaatc attgcagatg aatatgtaga tatggaattc gggacaggtg tagtaaaaat
    14281 cactccagca catgatccaa acgactttga ggtaggtaat cgtcatgatc ttccacgcgt
    14341 aaatgttatg gatgatgatg gtacgatgaa tgaactagca ggaaaatatg ctggaatgga
    14401 acgttttgct gctcgtaaag caatcgtgca agatctgaaa gaaatcggtc gattgattaa
    14461 aatcgatacg atgatccaca gtgtgggtca ttccgagcgg acaggtgtcg ttgttgaacc
    14521 tcggctgtct actcaatggt ttgtaaaaat ggcgccgtta gcgaaaaaag ccattgataa
    14581 ccaagaaaca gatcaagcag tggaattctt ccctccaaga ttcaatcaaa cgtttttacg
    14641 ttggatggaa aatgtccatg attgggtaat ctcacgacaa ttatggtggg gacatcaaat
    14701 tcctgcatgg tatcacaaag aaacaggtga aatgtatgta gggatggaag caccagcgga
    14761 tagtgaaaac tgggtacaag atcctgatgt tttagacact tggtttagtt cagctttgtg
    14821 gcctttctca acaatgggct ggccagatac ggaaagcgaa gattataagc gatacttccc
    14881 tactagcact ttagtaaccg gttatgacat cattttcttc tgggtaagca gaatgatttt
    14941 ccaaagcttg gaatttactg gcgaacgtcc atttgagaat gtcttgattc atggattgat
    15001 tcgtgacgaa caagggcgga agatgagtaa atcattaggt aatggaatcg atccgatgga
    15061 agtaatcgaa aagtatggag cagatgctct ccgctggttc ttatcgaatg gttctgcacc
    15121 aggacaagat gtccgcttca gctatgaaaa aatggatgct tcttggaact ttatcaacaa
    15181 aatctggaat gcttcacgtt tcgttatcat gaatatcgaa ggaatgaccg tagaagagat
    15241 cgatttatct ggagaaaaat cagtggctga ccggtggatc cttactcgtt tgaatgaaac
    15301 gatcgaacga gtaactgagc tatttgatcg ttttgaattt ggtgaagcag gtcgacaact
    15361 gtataacttt atctgggatg atttttgtga ctggtacatc gaaatgagta aagaagtact
    15421 ttatggagat gatgctgttt caaaacaaac cacccgtagc atcttagtcc atacacttga
    15481 tcagatccta cgtttattgc atccaatcat gccattcgtt acagaagaaa tatgggaaca
    15541 tattcctcat cagggaaatt ctctagtagt ggcagaatat cctgttgtac gtccagagtt
    15601 tgatgatgaa acagcttcta aagggatgga agtattgaaa gaattgattc gagccgttcg
    15661 aaatattcgt tcagaagtga atacaccatt gtctaaacca attacgttgc taattaaaac
    15721 aaacgatgct aaaatcgatc aatttttagt tgaaaataca agctatatcg aacgcttctg
    15781 taatcccgaa gagttgacga tatcaagtga gatcactgcg ccagatctag cgatgtcagc
    15841 tgttctgaca ggtgcagaaa tcttcttgcc gttagcagga ttgatcaata ttgaagaaga
    15901 aatcaaacga ttagaaaaag aactggaaaa gtggacaagc gaagtaaaac gtgtccaagg
    15961 aaaactttct aatgaacgtt ttgtttctaa cgcgccggaa gaagtagtag aggcagagcg
    16021 tgcaaaagaa aaagattatt tagagaagca aacggctgtc aaagaacgaa tcgaaagttt
    16081 gcgtactatt caataagaat agaaaaagag agtgtgaaat cagctagcta ttactaaaag
    16141 ctagctgatt gaacatcgtt acaggaaaga gtttgagcct cactcttagc ttcgattcat
    16201 aaggagatgt gacataaatt ttgtcgcatc tcttttttag tctactctta ttatgtagga
    16261 gaaggaggga ggtggatcat gtatcaagag tcaataaagt agttgataaa atcgttcctc
    16321 tctctgtttc ttttctcttt gtcatacttt tatttttctt atgtgttcta tttgttataa
    16381 taaaagagaa atgggggaat tgttgatgac gattgaagaa gcaatagaat ggatccatag
    16441 tcgattacca tttggttcac gtcctggcct tgaccgagtc gaagctttat tagaagtggt
    16501 agggcatcca gagagaaaag taaaaacgat ccatatcgct ggaacaaacg gaaaaggttc
    16561 tactgtagct tatttgagaa gcctattaga agaggctggg ctgacagtag gtacatttac
    16621 ttcaccttat atcgaaacgt tcaatgaacg gatcgcaatc aatggcaaaa gcattccaga
    16681 caatgatttg atccagcttg taaaaaaata tcagccactg atcaaacaac ttgatcaatt
    16741 tgaagcagta gctggtatta ctgaattcga gacattaaca gcaatggcat ttgattattt
    16801 tttgatgaag aaagtggacg tagcgattat cgaagttggc ttaggcggct tattggattc
    16861 taccaatgta gctgtaccaa tgctgacggg aataacaacg attggacttg atcataccga
    16921 aatacttgga gatacaattg aagaaatcgc tgcacaaaaa gctggaatca tcaaacaagg
    16981 cgttccagta gtaacaggaa atattgcccc agaagctttt actgtcatcc aacaaacagc
    17041 taaagcaaaa aatgcaccgc tttatgtata cggggaaaat tataaaataa actatctgca
    17101 tcctgatgag atgtggggag aagtatttga attttttaat gaaataggaa agatccctca
    17161 attaaccact ccgcttttag ggcaacatca agctgaaaat gcaggagtag ccatcgaatt
    17221 gtattttgtt tactgtcaat tggaaaagct gcctttccga gaaaaagatg ttttgaaggg
    17281 attgaagaaa tctcgatggc caggaagaat ggagcgtttg agtaacgagc cattagttgt
    17341 tttagatggt gcccataata cgcatgcaat gaaccggctt gttgagaatt tgaaaaaaga
    17401 attcaaagga tatcaaatca acattttgtt ttccgcctta cgaactaaaa atgttagaga
    17461 aatgctagaa cgattgcagc aagtaccaaa tgtccatatt tatttgacga cctttgaaca
    17521 tccaaaagcc atcgaattaa gtgaagtatc agatttagca agtgagagag tatcaatcgt
    17581 ttcattgtgg caattcggat tagcagatat attggaaaag atgacaaatg aagacgtatt
    17641 agttgtgacc ggttcacttt atttcatttc agaagttcgc catttattaa tacaattagg
    17701 aggagatcat gaagtataat gcagtgattt ttgacatgga tggtttgctg ttcgacacag
    17761 agttagtgta ttacgaagcc acacaagtag tagcagacaa gatgggtttt ccttatgata
    17821 aagacctgta tttaaaattc ttaggtgttt cagatgaaga agtatgggcg aattatcata
    17881 tgattttctc ggaatttggc aaaacaagtg tacaaagatt catagatgat tcataccagg
    17941 aaacgcttga acgtttttcg caaggagcgg tacaaatgaa gcccggtgtg ttggaactcc
    18001 tctctttttt agaagaacat cagattccca aagttgtcgc ttccagtaac caacgagcag
    18061 tcattgaact tttattgaag aagaacaatc tgtttgaacg atttgagacg attgtttctg
    18121 cagaaaatgt tcaacgagca aaaccagacc cagaaatttt cttgttagct aatcagaagc
    18181 taggtacagc gaagaaagaa acattgattt tagaagattc acaaaatggt attttagcag
    18241 cttctgcggc tgagatcccg gtagtcatgg taccagatct gcttccacca tcccaagaat
    18301 tgatcaaaaa aacagctggg gttgtttctt ctttacatga aatccctgca ttgttaaaat
    18361 aaaaatccat ctcttttcgt atcttatttt agataagata gaaaggagat gtttttttgg
    18421 gaaaacgatt gatcaaagaa gtacctacta gctctttacc aagggaacgt atggaaattt
    18481 atggcgcgga agctttatcg gatcaggagc ttttggcaat cttactgcgt accggtcaac
    18541 acccttatag tgtgatgtcg attgctggaa atctgttaaa aacgtttgga ggactggcct
    18601 ctttacgaca ggctactttg catgagttag aagagattcg agggatagga cgcgtgaaag
    18661 caatcgaaat caaagccttg atcgaattag gtcgacgaat tcagacggat catgaaagag
    18721 cggctccacc ggttcgtacc agctacgaat tggcccagca attgattctg gaaatgaaag
    18781 atcacaaaca agaacatttg gtttgtattt atttggacac gaagaatcaa gtgatcttaa
    18841 aaaaaacagt gttcatcggt tcgctgaatc agtcagtcgc tcatcctaga gaaattttcc
    18901 attatgctgt acgttattgt gcagcaagga tcgttttagc gcataatcat ccttctggta
    18961 atgttattcc atcgcagcag gatatgaatt ttaccaaaag gatacaaaag tgtggagaaa
    19021 tgatggggat cactgtactg gaccacttga tcatcggacg caaacgatat tttagtttga
    19081 gagaagaagg aatgatggaa gagaagtaag ttgctttctt gcaaaaaaaa tgaagcaagt
    19141 gtacaattat attgtaattt tgtttgggaa tagaagttga aacatctgtt gcacctctat
    19201 cgtacacgag caatgttaca atttaaatta gtgcttttgg cacgaggagg atattattat
    19261 ggaaaacgga acagtaaaat ggttcaacgc tgaaaaagga tttggattta tctcacgtga
    19321 agatggaagc gacgtattcg ttcacttctc agctatccaa ggtgacggat tcaaaacatt
    19381 agaagaaggt caagcagtga ctttcgacgt tgaagaatca gaccgcggcc cacaagctgc
    19441 aaacgttgta aaagcttaat tttatctgta aatcaaattg atggtagaca tcaattttta
    19501 cgagagaaca aagcgagtaa caaaaatacc tgccacaaga gacaatttct cttgtggcag
    19561 gtattttttt gtatgaaaaa gtaaataaat ttaagaggct atgaaagagc caccttgtat
    19621 caaggaataa gaacaaacaa aaatggcttc tcatgttttt catttgtttt ctcttatatt
    19681 caggacaagg tgctgatttc ataacctctt atttattctt catctggtgt cattaccatt
    19741 ggcaaaatca ttggatgtct ttctgtacgt tcgaataaga aaggctgaag cgcgccagtc
    19801 atcgcttctt tcaatttata ttctgaacag tcaggtccat tcaaacattc tcttaaagcg
    19861 tggaataaga ttcgctgagc ttcattgatc atttctcctg attctcgcat gtagacaaat
    19921 ccacgagaga gaatatctgg accagccaag atttcttttt tcttgatatc cactgtcgcg
    19981 acagctaata caagaccttc ttctgaaagc aggtgacggt cacgtaaaac gacattacca
    20041 atatcaccga tcccgctacc atcgatatac acatctccag ccacaaaatg cccagcagga
    20101 cgcgcagaat cgctagttaa cgctaaaaca tcgccatttt ccatgatgaa gcagttttct
    20161 gcaggaacac ctacatcttg tgctagagaa gcatgtactt tcaacatgcg gaattctccg
    20221 tggacaggaa taaaatattt tggcttcatc aaacgaagca ttaatttttg ttcttcttgt
    20281 ccaccgtgac cggaagtatg gatgttattg atttttccgt gaatcacttc tgcaccagct
    20341 tcagacagca aattgatcaa atgattgaca cttgttgtat taccaggaat aggtgaactt
    20401 gaaaagatca ctgtatcgtc tggctggatt tggatttgac ggtgagtacc attcgcgatg
    20461 cggctcaacg cggccatcgc ttctccttga gaacctgtac ataggatcat cgtttcattg
    20521 gcaggcaagc gattgatttc gtgtgcatct acaaatgtgc cgtctggaac tttgatatat
    20581 cctaaacgtt gaccgttgac gatggcattt tccatgcttc gtccgaaaac agcgatttta
    20641 cgaccagttg ccacagcggc atctgctgct tgctgcaaac ggaagatgtt agaagcaaag
    20701 ctggcaaaaa tgatacgtcc ttcgattttt gaaaagatct tcaaaataga atgcccaatc
    20761 gttttttctg atttagtaaa tgttggaatc tcagcatttg tactatcaga taaaaggcac
    20821 aagactcctt cttccccgat acgagccatt ttatgcaagt ttgctggttc gccaacagga
    20881 gtgaaatcga atttgaagtc tccagtcgct acgatatttc cagaaggtgt tttcaccacg
    20941 atccctaatg catctgggat actatgggtc gttctgaaaa agctgacaga agtctttcta
    21001 aaacggatga tcgtatcttc attgatctca tggagctctg catctcgaag caagccatgc
    21061 tcatctaatt tgtttgtgat caacgctaat gcgagaggac cagcatagat aggaatattt
    21121 gcttgtttca gcaagtaggg tattccgccg atgtggtctt cgtgcccgtg agtaatcacc
    21181 aaggctttta ctttgtgtaa gttttgtatg atgtagctgt agtcgggaat gacatagtca
    21241 attccaagta aatcgtcttc cgggaatttg atcccggcat caatcaaaat gatttcatct
    21301 tggaattgta ccccatacgt atttttcccg atttcaccta atccaccaat agcaaagacg
    21361 cctgtctcgt tatttttgat ggaaagtttc atattaaaac tccactaatg agaaatcagc
    21421 attttctttt tcgtactcta gatgacttcc ttctagaagt tgaacgaatt caatattgta
    21481 tggggtattt tcttccacac gttggcgagc gtctacatcg ctttttgcct caatgtaaag
    21541 agattttgtt tcttcccgtt tagggttgcg tacttttgtt tcttgataat aaactttata
    21601 gatcattgtt ttgtctcctt tatctgcgta aagcagtgat ttagctagtt gattttttta
    21661 gacaacacaa aacgagggga caagccacct ggtttttcat atgctttgac tagaatactg
    21721 gattcgagtc aaagagacaa gcctcgtttt tgcttatctg ttcggatatt ttttcaaccg
    21781 taccttctgt ataaaaaata tacaacaatt aattgttatt ctagcataaa acagcaaata
    21841 agtatagaaa tcaactagat tgaaagaaaa aatcaatgtt tatcatcccg ctataatcat
    21901 aatgactttt ttatgagaga agtgttctat taataggctc gttgtgctat catttttagt
    21961 aatgaaaatg taacaaaaga ttaaaaggag atgagaaagt gaaactaatg tggcattata
    22021 ccatgcgtta taagaaattc ttgtttttca atttcatatg cgtatttggt tttattttga
    22081 ttgaattagg acttcctacg attttagcac gtatgatcga tgtaggaatc cgcaatgatg
    22141 attttgatta tgtgaagcaa caagggatat ttatgatcgt tattactgta atcggtatta
    22201 ttttgaatat cttgttaggt tattttggtt cgaggattac gactaatatc gtagcagata
    22261 ttcgagatga tttgttcgaa aagatccaaa gttattcgca tcaggaatac gaaaatctag
    22321 gcgtatcttc attgatcact cgtacaacaa acgatgctta tcagatcatg ttgttcctgc
    22381 aaaatatttt acgtatcggt tttatggcac caatgatgtt cattgtcagt ttgtacatgg
    22441 tcatgcgtac aagtgtgaca ctcagtttgt atgtagtagg tgcattacct ttactattat
    22501 tggcagtcgt agcgattgct aaggtgtctg aaccgttatc taaaaagcaa caaaaaaact
    22561 tagataaaat caacagtatc ttgcgggaaa atctttcagg attgcgtgtg attcgtgcat
    22621 tcgtcaatga aaagtttgaa gaaaaacgtt ttgctgtagt caatgagaat tatgcttcta
    22681 gttcaaaaag cttattccgt ttgatggctg ttgcacaacc gggtttttat ttcttattta
    22741 atatcgtaat ggtattgatc atctggagcg gggcagtgca aatcgatgca ggcgagcttc
    22801 gtgtaggaga tttgattgcg tttatcgagt atatattcca tgccttgttc tcattcatgt
    22861 tatttgctag tgtgttcatg atgtatccaa gagcagctgt ttcagctgga cgtatccaag
    22921 aagccctaga tgccaaacct tcgattgtag aagatccaaa tggtatatct gatacaaaaa
    22981 ctaaagggta tatcgaattt caaaatgtaa catttgctta tccaggccat tcgcaagagc
    23041 cagtgatacg aaatgtcagt tttacagcct ctccaggaga gaccgttgct tttattggaa
    23101 gtaccggtag cggaaaatct acgttgatcc aattgatccc tcgcttttat gatgtgagtg
    23161 aaggaaagat cttgatcgat ggagtagacg tgagagatta tcggctaagc aagttgcgag
    23221 ataagattgg gtttattccg caaaaagcgc tgctatttac tggaacgatt gcagataatt
    23281 tgcgatacgg gaaagaagat gctacgcaag aagaaatgga acgtgcagct gagattgctc
    23341 aagcttcaga attcatttct cgaaaaccag atggatttaa tgaacattta tcagaaggtg
    23401 gttccaattt ttcaggtgga caaaaacaac gattggcaat agccagagcc atcatacgcc
    23461 gtccggaaat ctatattttt gatgacagct tttctgcatt agattatcaa acggatgcga
    23521 agttacgtgc tcgtctgaaa aaagaaacga cagaatctac tgtattgatc gtagcacaac
    23581 gtgtgggaac gatcatgcat gcagataaga tcatcgtatt aaatgaagga gaagtcgtcg
    23641 gtatggggac gcatcgggag ttgttggaaa aatgcccgat ttattatgat attgccgctt
    23701 ctcaattatc aaaggaggaa ttagcatgaa aaaaaccttt tcttccttaa aacgtttagc
    23761 tcgatatatc agaccgtatc gtgagacttt tcttttagtg atcctattta cagttctgac
    23821 agtggctttc aacgtggctt tgccatatgt tacaggactt cctactacag aaatcagccg
    23881 taatattgcc aatggcgaat caatcaattt ttcttatata ttcaaatgtc tcatttggat
    23941 cacagttgtt ggaatagggt attgtatttc gcaattactt tctggtgttt tgatgacaaa
    24001 tgtcgttcaa agtgccatgc atgatttgag aagagacata gaagaaaaaa tcaatcgtct
    24061 tccggtttca tatttcgata aaaaccagca aggaaatatt ttgtctcgtg tgacgaatga
    24121 tgtagatgca gtcagcggtg ctttacaaca agcttttatt ggtatcgtaa atgcgatatt
    24181 gggaattacg ttggctgcag caatgatgtt ttatatccag ccgatgatgg cgctgatctc
    24241 tatgatcatg attccaacat cgatctggat ctcaaaacga gtgatcaatg cttcgcagaa
    24301 atattttcaa agcatgcaga attctcttgg tgagctaaac ggctacgtcc aagaaaatat
    24361 gacaggtttt agtgtattga aagtatatgg acgcgaaaaa gaaacattgg aaggattcaa
    24421 aaaagttaat cacagcttga aatactttgg gtttcgtgca gcattcattt ctggcttgat
    24481 gatgcctcta gttcaattaa ctgcctatgc tacttatatt ggtatggctg ttctaggaag
    24541 cttttatgtt attactgggg cgatcgttgt cggtcaacta caagcattca tccaatatat
    24601 ttggcaaatc agtcagccaa tggggaacgt gacacaattg tcttcagttt tgcaaagtgc
    24661 atcagcggca acgaaacgtg tattcgagat tttagatgag ccagaagaaa aagaaaacaa
    24721 agtagatgct tcactgccag aaacgatttt aggagatgtg acattcgatc atgtcgattt
    24781 tgcatatgat ccgaagaaac cattaatcca agatttgaat tttgaagtga aagctggtca
    24841 aaccgtagct gtggtcggtc cgaccggtgc cggcaagacg acattgatca atctattgat
    24901 gcgtttttat gacgtagaca aaggagcaat aaggattgat ggaatcgaca ccaaatcaat
    24961 gagtcgcagt gatgtgcgtt cgctatttgg tatggtcttg caagatgcat ggctttatca
    25021 aggaacgatt gcagataata tccgcttcgg aaagcttgat gcgacagatt atgaagtagt
    25081 tgatgctgcg aaaacagcaa acgtagatca ttttatccga acgatgccag atggatatga
    25141 tatggccatc aattctgagg gagacaatgt gtctttagga caaaaacagt tgctgacgat
    25201 tgcccgagca gtggtctccg atcctaaaat cttgatttta gatgaagcaa caagttcagt
    25261 agacacccgt ttagaagcgt tgattcaaaa agcaatggat cgcgtgatgg agggacggac
    25321 aagtttcgtc atcgctcacc gtttatcgac aatccgtgat gcagatctga ttctagtgat
    25381 ggatcaaggt cagatcattg aaaaaggaac gcatgagagt ttattggcac aaggtggatt
    25441 ttatgaaaaa ctgtataaca gccaatttgc tgaggaagca gaataagtca tataagaata
    25501 aaacagttta aaacttattt tttgttctct attcttgata atggtaaagt aaagttatca
    25561 agaaaagtga ggacacggag atggaacagt ttttaaatga aaaaattact gaagccaaaa
    25621 gagaagttga tcgtttaacg cacgtctgtc agacgaagcc ttttgattca gctgattttt
    25681 ttgaaaatga gtttcaaatc gaacgcttgg cagcacaaat agaagtgtac gaattaattt
    25741 taaaaaaagt aatagaagaa aatatagatg tacaataaaa acaggtaaaa cgtcagttac
    25801 cagatactgt aaatagacat ctggcctgag caggttttac ctgttttttt tcttatgaaa
    25861 taaatatatg ctatgatacc tttattcaat aggattagtt tctacaaaaa atgaaaggag
    25921 aaaaataaat atggaacggt ttatagcatc aaatgccaca gtgattggtg atgtgacatt
    25981 gtcagaagat gtgacgatct ggtatcaagc tgttttacgc ggggacagta attggataaa
    26041 aataggacaa agaacgaata tccaagatgg tacaatcatc catgtcgatc atgatgcacc
    26101 tgtagatatt gcggagaatg tgacggtagg acatcagtgc atgctacatg gctgtacaat
    26161 cgaaaaaggg gcgctgatcg ggatggggac cacgatattg aatcatgcgg ttattggtga
    26221 gaatagtttg attggtgctg gatcattagt aacagaagga aaagttattc ctcctaatgt
    26281 cttggctttt ggtcgcccag ctaaagtgat ccgtcctttg acaaaagaag aaatacaaaa
    26341 aaacaaagaa aacatccagc attatgtcga gataggtcgg atgcatgcgc aaggaaaatt
    26401 caaggagcaa agataggcag tagcatccat aaaataaaaa aggatggtca gtttgagttg
    26461 accatccttt tttatttggt ttagataaga gagcttaaaa aagcttcctt tgaaacagat
    26521 taatttaatg aagaactgtt ttccatgact tcatcgatca atccatatgc ttttgcttgt
    26581 tcagctgtca tatagttgtc acgatctgta tctttttcaa tcacttcaag cggttgtcca
    26641 gtgcgttcag ctaaaatttt gtttaagcgt tcgcgtgttt gcaaaatatg gcgtgccgca
    26701 atttcgattt cagttgcttg cccttgagcg ccgccaagag gttgatggat catgatctca
    26761 gcatttggta aagcaaatcg tttacctttt gtaccagctg tcagcaagaa gcttcccatt
    26821 gaagcagcca tacccataac aattgtttgg acatctgctt tgacaaagtt cattgtgtcg
    26881 taaatagcca tacctgctgt aactgaacca ccaggagaat tgatataaag atagatatct
    26941 ttttcagaat cttgagcgtc taagaaaagc aattgggcga tgatcgaatt agcaagatca
    27001 tctgttactt ggccgcttaa catgatgata cggtctttca ataaacgtga gtagatatcg
    27061 taagctcttt cacctcgtga agattgttca atgactgtag gaattaaatt cataatagag
    27121 ttcctccttg atttcgttta gaaatagtgt accataattt agtttctaat acctaaaatg
    27181 tagtatacat ttttggtcaa aaaaggtcaa ataaaaaact taggcaaatt agtcgcataa
    27241 acccgcttta tggtatgata agcagagaaa caaaacaagg aggaacaaca gattgatagt
    27301 tggtattact ggccttatcg gagtgctgac aggtgctgta gttgtctata tcggcatgat
    27361 actgcaaatg cgtcacttgg aaaataaaga acagcgtaga cgtgaattgg aattgaaagt
    27421 caaagaaatc gagacgctga atcaattaaa taaaaagatc aacgagatct tgcaaaaacg
    27481 gacaacgctg atggaagaat atgtcagctt tgatgcattt gatgattgtt atatcacgat
    27541 tgacgatttt gcgtatctcc aatcattttc agcgcaaaat agtttttatc tgccaaatta
    27601 tattttagac gagctattta agaatatctc ccatcgaaaa gtgattttat ctccagatga
    27661 aacaatgaag attggtggat atgcttataa aggcggacgc gtgattttag agaattttac
    27721 ggatagtctg actgaaatga tcaatgaaaa aaaagctcag ttgaagacta tgacgcgcca
    27781 gccgattggt ttcttctcga aagatgatgt ttggagctga tattttgaag cattgccgtt
    27841 gcacatgaga gcgtggtaat gctttctttt tttcttaatg atacaataga actgaacgga
    27901 gtgaagaaag atgttacaaa tctatgccca tcgcggaagc agcggcaccc acccagaaaa
    27961 tacgcttcct gcatttgcag aagctgtacg agtaggtgca gatggtatcg aactagatgt
    28021 ccatctaagc aaagatggtt atttgatcgt tatgcacgac gaagaagtgg accggacgac
    28081 taacggtaaa gggctgattc gagaaaaaac attggaagaa ctcaaaaaat tgaatgcagg
    28141 gggcaaattc agcaaagaat ttccagcagc taaagttcct gaattgagag aagtgttagg
    28201 actgctcctc caaaaaaatt accgagggat gctaatgatt gaaattaaaa ctgatcagta
    28261 tgaatatccc ggtatcgaag aaaaattagc cgaaacatta aaaagtagag attggccttt
    28321 tcaacatgca tattgcagct ttcatttgga ttctttggaa aaaatcgcat tactggaacc
    28381 ggagatacag ctggacttat tgatgagtac ttctgataaa aagatccaac tagcagcaga
    28441 aaccccttat atagaaggag tacatccaaa aaatacttgg ttgttcgaac atccagtcca
    28501 ggcagctgat tttccaaaag caattcgtgc atggacgatt aacgatgaag aagaaatggc
    28561 caaatgtcaa gaactcaaaa tagatggaat aataactgat tttccggaga aagctcttcg
    28621 gtggaaacaa tcaacgaaaa gtaggtaaaa tatggaaaaa gtattagtga tcgtcggtcc
    28681 gacagcagtc ggtaaaacag cgctaagtgt ggaactcgca caactatttg gaggagagat
    28741 catcagtggt gattcattgc aaatctataa aaatctggat atcggtacag caaaaatcaa
    28801 agaagaagag atgaataatg ttccgcatca tttgattgat gtgatcgaac ctactcaacg
    28861 ttactcagcc gctgattttc agcattcagg tcgacaattg attaccgaga ttgcgaaccg
    28921 agggcaccta cctatcatag ctggtggtac gggtctctat atccaatccc ttctatatga
    28981 ttatcagtta ggagcgacag aagaagcgaa tcctgagatt agaaaaaagt atgaaacgtt
    29041 agctgaaaca attggttccc aagcattatg gaatcagttg aaagaaaaag acccggcagc
    29101 tgctgagaaa atccactgga acaatcaacg caaagtgata agagctctgg aagttttaga
    29161 gacgacagga catagtatca cggcacctaa agaagagcca aagcaattgt atgattacct
    29221 catgattgga ttagatacga agcgtagtat tctttatgaa cgaatcaatc aaagagtcga
    29281 tttgatgcta gcagaaggtt tagtagcaga agcgcgtaag gtgtatgagc ttggagaagt
    29341 ccaggccagt caaggtattg gatataaaga atttttccct tattttgaag ggaaagaaac
    29401 attggaagaa gcaacagaac aagtcaaact acattcgaga agatacgcga aacgtcagtt
    29461 gacgtggttt cgtaaccgtt tggatgcaca gtggtttgat cttataagtg agccaaatca
    29521 aaaaagtcag attgagcaaa caatcaaaaa atggctggag gcggaataaa atggaagaaa
    29581 aagtgatcat cgtaggggta gaaaccgaag caaaccagcg ttacttttca gagtcaatgg
    29641 aagagctagt tcagctgacg aataccgcat caggagaagt cgtattcaca attacacaaa
    29701 agagaccaca agtggatcgg caaacgatta tcggtaaagg gaaattacaa gaattagttc
    29761 aacaagcgga tgcgcatgaa gcggatttga tcatctttaa tcatgagcta actccgagac
    29821 agagccaact gattaccgat gctgttggtc ttccggtaat tgatcgcgtg cagcttatct
    29881 tagatatttt tgcgatgcgt gctcgttcga aagaagggaa gctgcaggtt gaacttgctc
    29941 agttagaata cctgctgcct cgtttagtag ggcaagggaa aacattatct cgtcttggcg
    30001 gtgggatcgg aacaagaggg ccaggtgaaa caaagttaga aacagaccgc cgacatatac
    30061 ggaataaaat ttttgctgtt aagaaagaat tgaaagaagt agaagctcat cgagagcgca
    30121 atcgccaaaa aagaaaaaat agcgagattt ttcagattgg acttattggc tacacgaatg
    30181 ccgggaagtc aactatcttg aatctattga cacaagcgga tacttattcg aaagatcaat
    30241 tgtttgcaac gttagatcca ttgactaaga gatggcgttt tgctgaagga tttgagatta
    30301 ctgtaacgga taccgtcggg ttcatccaag acctgcctac gcaactgatc gatgctttcc
    30361 attctacatt agaagaaagt caaaatatgg atcttttgct acatgtagtc gatgctagtt
    30421 ctccagatcg tattttacag gagcagactg tcttaaaatt gatggatgaa ctgaatatga
    30481 aagaaatgcc gatactgact gtttacaata aggcagacca gatcgaccca gcgatgttca
    30541 cgcctacgct attcccaaat gtattgattt ctactcagac gaaagaaggt aagatggcgt
    30601 tgatcgaagc aatcaaacgt cagctgatgg aactaatgac cccttataca ctttctgtgc
    30661 caagcaacga aggacagaaa ttaagcgagt tgagaagaca aacaatggtt ttggacgaag
    30721 cgtacatgga agaaacaaac gaatatgaag ttaaagggtt tgcaaaaacc aattcaaaat
    30781 ggttaagaaa aaaccaataa aattttattt ttttaa
//
LOCUS       DACCXO010000024        29111 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.025, whole genome shotgun sequence.
ACCESSION   DACCXO010000024 DACCXO010000000
VERSION     DACCXO010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.025"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..1031)
                     /locus_tag="IVY70_001009"
     CDS             complement(<1..1031)
                     /locus_tag="IVY70_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7958153.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="IVY70_001010"
     CDS             complement(1113..1802)
                     /locus_tag="IVY70_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP7958154.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="IVY70_001011"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="IVY70_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP7958155.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /locus_tag="IVY70_001012"
     CDS             complement(2329..2832)
                     /locus_tag="IVY70_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP7958156.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="IVY70_001013"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="IVY70_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP7958157.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="IVY70_001014"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="IVY70_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP7958158.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="IVY70_001015"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="IVY70_001015"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP7958159.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="IVY70_001016"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="IVY70_001016"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP7958160.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7463)
                     /locus_tag="IVY70_001017"
     CDS             complement(6444..7463)
                     /locus_tag="IVY70_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP7958161.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7636..8523)
                     /locus_tag="IVY70_001018"
     CDS             complement(7636..8523)
                     /locus_tag="IVY70_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7958162.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="IVY70_001019"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="IVY70_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP7958163.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="IVY70_001020"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="IVY70_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP7958164.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="IVY70_001021"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="IVY70_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP7958165.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /locus_tag="IVY70_001022"
     CDS             9754..10398
                     /locus_tag="IVY70_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7958166.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="IVY70_001023"
     CDS             complement(10454..10957)
                     /locus_tag="IVY70_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP7958167.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="IVY70_001024"
     CDS             11554..12519
                     /locus_tag="IVY70_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP7958168.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="IVY70_001025"
     CDS             complement(12618..13790)
                     /locus_tag="IVY70_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7958169.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="IVY70_001026"
     CDS             complement(13777..14922)
                     /locus_tag="IVY70_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7958170.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="IVY70_001027"
     CDS             complement(14936..15868)
                     /locus_tag="IVY70_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7958171.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="IVY70_001028"
     CDS             15985..17064
                     /locus_tag="IVY70_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7958172.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17690
                     /locus_tag="IVY70_001029"
     CDS             17061..17690
                     /locus_tag="IVY70_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7958173.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17800..18027)
                     /locus_tag="IVY70_001030"
     CDS             complement(17800..18027)
                     /locus_tag="IVY70_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7958174.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18130..20718)
                     /locus_tag="IVY70_001031"
     CDS             complement(18130..20718)
                     /locus_tag="IVY70_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP7958175.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20738..21397)
                     /locus_tag="IVY70_001032"
     CDS             complement(20738..21397)
                     /locus_tag="IVY70_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7958176.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="IVY70_001033"
     CDS             complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="IVY70_001033"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP7958177.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22170..23036)
                     /locus_tag="IVY70_001034"
     CDS             complement(22170..23036)
                     /locus_tag="IVY70_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7958178.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23145..23786
                     /locus_tag="IVY70_001035"
     CDS             23145..23786
                     /locus_tag="IVY70_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP7958179.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="IVY70_001036"
     CDS             complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="IVY70_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP7958180.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25227..26126)
                     /locus_tag="IVY70_001037"
     CDS             complement(25227..26126)
                     /locus_tag="IVY70_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP7958181.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHILVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26128..26925)
                     /locus_tag="IVY70_001038"
     CDS             complement(26128..26925)
                     /locus_tag="IVY70_001038"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP7958182.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26903..27607)
                     /locus_tag="IVY70_001039"
     CDS             complement(26903..27607)
                     /locus_tag="IVY70_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP7958183.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27738..28313
                     /locus_tag="IVY70_001040"
     CDS             27738..28313
                     /locus_tag="IVY70_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP7958184.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28431..29108
                     /locus_tag="IVY70_001041"
     CDS             28431..29108
                     /locus_tag="IVY70_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP7958185.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8567 a         6168 c         5232 g         9144 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcta gtcttatacg aaacccatcg tcccgatgtt ttgctgacgg
    17221 acatccgcat gcctaaacaa acgggcatcg atgctgccaa agaaattttg gctaaatttc
    17281 ccaaagccat ccttttgctt ttgacgacat tcaaagatga agaatacatc cgcgaagcat
    17341 tttccatcgg tgtcaaaggg tatttaatca aacagaactt gcaagcgatt attccttctg
    17401 tcaaatccgc ttataatgga caggccgtat ttggtaatga aatcatcgag actttcactc
    17461 aaatgatgaa gaataaaccg acgattgata gtcatgctta tgcttctttt agtgaacgtg
    17521 agcttgcaat catcaaagaa gtagctgccg ggaaaaacaa taaagaaatt gcagatgcct
    17581 tgtacttgag tgacggcact gttcgcaatt acatcagcca attactagaa aagctagatt
    17641 tacgggatcg cacgcaattg gctatctatt tttatcagca tttgcaataa gtcttatcta
    17701 acacaaaaaa acctgaatga tgctccttcg catcattcag gtttttttca catcttctat
    17761 tcaattgtcc cgcaaaccat ttcctcacga ttgaaagaat caaggattcg cagggctgcc
    17821 aggattcgtt ggattgttcg gcgtgttagg cggaaaaata ggcgccttgt ctaacgtttt
    17881 ttctagtttt gattcttcac gatcgtctcg ttctacatca ggactttcat tcaatttgaa
    17941 ttctttttcc atctcatcta actttttaaa gtcattgcca gattcttttg ctaatttcgc
    18001 taatttatct tcggtttctt tagccataaa gtaacgcccc ccttttgatt tcagcttagc
    18061 atatcacact gaaaaaacaa aagaaaacgc taaccaacga tcatttttag ctatttctca
    18121 tctttaaaat caagcatttt ttgccggtat aaaatcgtag ggagaactgt tttccatacc
    18181 aatcatcgga tctactaact ggttatcgat cacttctgct gtaagaacga aatctttaga
    18241 aacagcaggt tctacctctt ctttcttttc ttctccaaca aatgaagaaa acagatctgg
    18301 ttccactgtt tccattactt ggtttttcgt ttcattcgtc ccactgtcat cttctacagg
    18361 tagcttttct tttacaaccc gtggctcttt tgtatagttc ccatcttcaa atggatcatt
    18421 tccgaccaaa gaatcttcat aagctgctag tttggtccgg agagtgatat tcattttatc
    18481 attatccatg atccttgatt tcaacgtagt catccggctt gctgattcat tcgctacaca
    18541 agtgacatag gcttgctctt cccctaacaa aatcaacagt tccttactgc gagtcactgc
    18601 cgtgtagagc aaattacgct gaagcatccg ctgatattga tggaccatgg gcaaaataac
    18661 catcttgaat tcactgcctt gtgatttatg gattgagcaa caataagaaa gagtgatttt
    18721 attccattcg tttcttttat aagtgacctc atttgcatca aactggataa ctaattcatc
    18781 cactttatct tcagaatctt ttgccaagat gatacccgtg atttctccca tatctccatt
    18841 gaatacattc aattcgggag tattgactaa ttggaggact ttgtctccta tccgataaac
    18901 ggtgtcgttc caatgtactt ccttttttcc aggtttccct ggattgaata tctcctgcat
    18961 cattttattt aacgcatcga tccctgcagg tccccgatac attggtgcta atacttggat
    19021 atcttgtgca cggaatccct tatttttcgc tttttccaca attttagaga tcaagggttc
    19081 aatttgataa gcatcacttc ggaaatagga acgatctttt tgattctttg tgaaatcttt
    19141 tggcagacgt ccttctttga tctcatgggc aagaggaata atactagaac catcaccttg
    19201 ccgataaatc tgattgagtt ccattttggg aatttgttcg atttccaaaa gatcatggag
    19261 tacttgacca ggaccgacag agggaagctg atctttgtct cctactaaga taacttgcat
    19321 attcgtcgga atcgctttga ataatgtgtt agccagccat gtatcgacca tagacatctc
    19381 atcaacaatc aaaagtccgc cttctaattc tttcgtactg actgaaggtg ttttttcccg
    19441 tccattcagc cccagaagac gatggatcgt actggcaggc agacctgtcg tttcgttcat
    19501 tcgtttagct gctcggccag ttggtgctgc aagtagaata ggaaaaattt cttgggtata
    19561 ttttgaagga tccaattcca gtccgttcaa ctctgcaaaa agattgacga ttccgttgat
    19621 cacagtagtt ttccccgtac ctggtcctcc tgttaaaata aatagagggg aacggattgc
    19681 ttcagcgata gcttgttctt gcgaaggtcc atactgtatg ccgaatattt tttcgatacg
    19741 ccgaatattc ttctgcactt ctttttcagg ataatggatt tcttttttac gactgagaag
    19801 gcgctggatc gaattaccga tcccccattc agaaaagtac aaactgtttt cgtaaagatt
    19861 agtctcttct tgttgtatct ttccttcttc aacaagttgg ataattacag tagcaacctg
    19921 atcaggctgg atttcaattg ggcgacttgt ttctaaaata cggatcgttt gctgaagcaa
    19981 tgtctccgcc ggtacgtatg tatctcctgt ttgaactgca tgctgaaaaa tctgatgcaa
    20041 gatagctgct cgaattcttc tcggtgaatc tgctgcaata ccgatttgtt cggctagatt
    20101 atctgcacgt ttgaacccga ttccttctat gtcttcgact agctggtacg gattttcttc
    20161 gatgatatcc aaagactcgt tcttataggc ctggtagata ctgaaagcta actgactacc
    20221 gaacccgtaa cgattcagtc ccatgatcac ttgatccatc ccgtggttta gccgaatcgt
    20281 ctcagcaagc atttctcttt ttggcttcgt caaaccggag atttgtttta ataccgaagg
    20341 atcagcaatg attttttcga tagcatcttc tcccagcaat tctacaattt tttctgctgt
    20401 tttcttcccg attcctggga atttttcgct ggaaagatac gctacgatcc catttgcagt
    20461 tgttggcttt tcttgctcat atctttcgag tttaagctgc cttccgtatt taggatgatc
    20521 cactaattct ccataaaaac gataagtctc attttcttga atatcgccga aacttccggt
    20581 gactacaatc tctttttcac ggtaatcagc tgtggtttca ctgatgtgta ccaaaagcac
    20641 tttgtaaaaa ttactaggat tttgaaaaaa aatcgcggca actgtaccaa cgaaataagg
    20701 catttcttct ggctgcatcg gaagccgcct ccttcttcta tagtaaaggt tctggtgctg
    20761 tttcagttcc taaaaaatct gtttggttcc atactttcag ttcatatggc aataatctgc
    20821 aaggctcttc agtctctaaa atcgtcaaac tactattgaa caatccaccc atttcacgca
    20881 actcacttag ctcttttcct gtcatccatt gaatggctgc agttaaagaa gctccatgtc
    20941 ctacgaataa aagtgggcca tctttatgtc gggcaaccgc atctgctaca acagtttcga
    21001 tacgtgtgat tgccgattca atcgtttctc cgtgaaaagg agaaggatca tacagatcta
    21061 aatgattacg caaattcggc agattatccg gatatttctg gtacatttca tcgatcgact
    21121 gaccttctaa agtacccagc cctagttctc ttagcttatc tgtataaaaa atctctaccg
    21181 gacgagcaag ctctttgttg ataccttctg ctgtatcacg tgctcgcttt gctgtactgg
    21241 aataaattgc tttaaaagga acatatttta cctttttccc aaatgctttg atttcttcgt
    21301 aactttgtgg caaaagcgga gaatcgccat tgctcccttg aaaacgtctt tcctgattcc
    21361 attgtgtctt cccatgtcgg gtaaaatata gttccatacg cagcaaactc ctgtctttct
    21421 tttattttac tctataataa actgtctaat ccagcttgtc attttcatag tgatgcgtca
    21481 attctaaatt agggaatcct tgctggcgaa gtgcttcata cactatcatt gcagctgtat
    21541 tcgataaatt caatgagcgg acatgctcat cattcatagg gatgcgtaaa cacttttctt
    21601 cgttttcacg cataaatgtt tctggcaaac cggttgtttc ttttccaaat aaaaagaagt
    21661 gctcttctcc atcagcaaaa ttttcgtcac tatatacttt atgggcaaat tttgagatca
    21721 aatgtagttt acgattacct aaaaatgtta aaaaagcttc taaattactg tggtaagtaa
    21781 tatttacatc attccaataa tccaaccctg cccgttttag atgtttgtca tcagttgaaa
    21841 aacctagtgg ctcgattaaa tgaagcggag tattcgtggc tgcacacgta cgggcaatat
    21901 ttccagtgtt tgcagggatt aatggttcaa ataaaacgat atgattcatc aggatcattc
    21961 ctctcatatt tatgttcgtt tctattttct gacaatttga tttttgcata aaaaaaaacg
    22021 tatcaactcg gtgtcagtca ctgcgagtcg ttacgttagt gaagctcttc tcactaactt
    22081 ttatcagaac aggaacctga taaaaaccaa tgaaaaaaca acttgttgct aatataacac
    22141 cctcaacttt tttttgcaac ctctactcat taaaaactgc tagaaaaacg ttttctctta
    22201 gttcctttta ataattctat gtcaattgta atcttcttca aaggattctt atataagtct
    22261 gctttgattc gctcttctac ctgccattct aaaggtgaca tggccaaaag atctttttga
    22321 ttttcctctg gtatctcaaa ttcataggtt acacgctgac gttcagttaa tttcattgct
    22381 tcagcaaact tggctactac acgttcattg gaataactct gtttttcagg ttgattcgga
    22441 taaaaagcct gacgtagttc ctttaaataa ttttcttgtg gcaccacctt caacacttct
    22501 ccatcttctg agagtacacg ttggaattct tggtaatgag aaggcgaaaa gatatttaaa
    22561 accgcgttga atgtttgatc agcaaacggc aaattggtca aatcagctac acaccaaaat
    22621 gcttcggcag attgttctgt agctgcgtag actccttctt ttgaaatatc aaatccaaca
    22681 gcattttgaa tctttttcaa ttttgaaagt tcattcaaga agctaccttc tccgcagcca
    22741 acatctaata gcgagacttc ttcaggtaaa tgctgcgcaa tagtctcaat aacgggctga
    22801 tacatgccag attggatcat tctacgcctt ggaacgaaca ttccttgatc gtattctgtt
    22861 ttaaccgcat gggataaaaa ataaagtgtt ccttttttgg aaaggtcaaa acggtgatta
    22921 tttttgcata tcaagccatt atcagacttt tttacttctt cttggcaaac cggacagcgg
    22981 aacagctgat gattttctgc caaaaactgg cgtcctcggt caattttttt taacatagtt
    23041 ccccctcatt tctgttttat cttaccgtta gatgctcact ttggcaactg ctaaaaatat
    23101 gatacaatgc ctagaagaaa gtgaaagata aaggagaaga ttagatgatc aaagaatttt
    23161 gcgctgaaaa ctacacatct attcctctag caattgctag aggagccgat cggatcgaat
    23221 tgtgtgacaa cttagcggtc ggtggcacaa ccccgagtac tggtgtgatc gaagaaacgt
    23281 taagttatgc tggggaaaaa gatatccccg taatgacgat tattcgtcct agagggggcg
    23341 acttcgtcta taacgatatc gaattgaaaa tcatgcatac tgatttgatt gaagcaaaga
    23401 aattagggac ggatggcgta gtatttggtt gtcttactcc ttcaggctgg ctagacgaag
    23461 aggccttaga actattgatc gaaacagcag aagggttgca gatcaccttc catatggctt
    23521 ttgacagtat tcctgaagat cggcaattcg aagcaatcga ttggctggta gaacatggtg
    23581 ttgaccgtat cttgactcat ggcggtcctg ctgacaaaac tattgtagaa aatttcccac
    23641 ggcttaaaga attgattgcc tatacagatg gccgcttaat cattttgcct ggtggtggtg
    23701 tcactgcaga gaatgcagca catgtagcta aagaattgaa cgtaagcgaa gtacacggaa
    23761 ccaaaatcgt ggatcttcag ttataatctt ataaacaaaa aaacatttgc aacaggcata
    23821 atgcttattg caaatgtttt tgttttttta cataaaatag cccataaata aagaagcaat
    23881 attgaaataa atcagtacac tcgtcaaatc gctcaaagtc gtgataaagg gaccgcttgc
    23941 tactgctgga tcaaatccta gacgatccat tagcatcggg attaaactac ctgcaagatt
    24001 cgccactgtg attgcaaata gcatagccat ccctatgaca aaccccaaga taaaattatg
    24061 tttccaaaaa cctacgatta gaaaaatcgt gatccccgta acagctcctg tgacaacgcc
    24121 ggtcaagact tcagccaaaa caagtcgaac aaatccatga tctttctcat cagaaatcgc
    24181 aagtcgacga acagccacag ctagcgactg ggttccagca ttaccggcag ttcctgtaat
    24241 cagcgaaatg aagactgcta aaatactcgc ttcacttacc aagtcttcat agtgactgat
    24301 taaagaagcg gtcgacatac ctaaaaacaa cagagtgatc agccaaggca aacgtcgaga
    24361 cgctgccttt actggatttt cattaatttc ttccacattg accccggcaa gtcctgaata
    24421 atcgctagca gcttcatcat caataacgtc gatgatatca tcgactgtga cgattcctaa
    24481 caaatgatca tcataatcgg taacaggaag agctaagaag tcataatctc taaatgtttg
    24541 tgccacatct tcttggtcgt cgcctacatg aactgaaagc actcgttcgc tcatcagatc
    24601 agaaatcatt gcgtcgtcat cattcacgat taggtcgcgc agagaaataa cccctactag
    24661 ctgaccttct tgattaacga catagatgta atagattgtt tcagctacat ctgcttcgtt
    24721 cttcagcacg tacatcgcag agcgaacagt ttgattggct acgatcgaaa caaattcagt
    24781 tgtcatgatc gcgccagccg tttcatcctc gtaatggaga agttccttga tttcactggc
    24841 atcgtctgcc gacatcaaac ttaaatattt agcaatttgc tttttatcca gcgtattaag
    24901 caagtcaaca gcattatctg tatacatctc agaaagcata tcagctgcat agctaggacg
    24961 catttcatct aggtattctt tcatatgttc gttgtcttct tcaatcacat cgaacatatc
    25021 cgctaattct ttaggcgaca gataggaata gatcagctgc ctgttctctt ctgtaagaga
    25081 ttggtagaat tgcccctgct catagatatg catctctaaa aaactgtttc tgaatgcctg
    25141 aatattattt tcccttaaat cttgagtcaa ctgggcaaat ctttcctcta attcttgatt
    25201 ttcatccaaa ccaatctcac ctcgccttat tcttcaaacc gtctcacaac tgcctccatg
    25261 tctgcggcta aaggctgctt gaactccagc aactcttctg taaatggatg gtaaaaacgc
    25321 agatcataac aatgaagggc ttgtcttttt attcccagat ccattcgccc gccatacata
    25381 tcatcaccga gcagggcaca gccaattgcg gaaaaatgaa ctcttatttg atgtgtcctt
    25441 cctgtatgca gctggatatc aactaacgcg atttctgtta gtcgcttctc tatccagtac
    25501 tcagtctgtg ccgaacgtcc ttcatcagag accattcgct tcaatagcga agaataatct
    25561 cgggcgatcg gcaaatcgat atgtccatgt gccgataaat ctgaaagcct tccactgact
    25621 aaggcctgat attttttcac aaacttcttt tcacgaagtt gcatatctaa ttttgcgtgc
    25681 gcaaatccat gctttgcaaa aagcatcaag ccagatgtat cccgatctag tcgcgtcacc
    25741 acatggatta cttggttttc gtaaccttgt tttttataat aagcctttac acgattagcc
    25801 attgtcccat ttggatggta ttgggcagga atagacgaaa cgcctgctgg tttatttact
    25861 accagaatgt gttcatcttc atacacgata tcgatcggtg tttcatccaa aagtacggtt
    25921 tcatgttctc cttcagcagg aatcacaatg gttaccttat catccttagc tagagaaaac
    25981 aagacatttt ctacttgatc attaactttg atttgaccgc cttgaaattt tattttcgct
    26041 aacagaccct ttgagatccc tttttctttt aaaaaatatt ttacttgctg agaatgatct
    26101 ttctcataat cccatgtaaa ctgcatatca atcctcacta ataaaggcat ctttcacacg
    26161 atgccaaaaa tgcatatgtc gataagaagc aaaatggatc cgttcttctg ctattcgata
    26221 ataaattgag cggatatttg cttttgcgac attcaattga tcaaccgtta caagataatc
    26281 ttctgtattt tccaatttta tttctagcca ttcatcatgg gcaatcacga tcggagaacc
    26341 caaagtacga aacactcgat tattcagtga agcaatctct gccaactgaa aggcattgat
    26401 actaggatgg atcacagcac cgccgacact tttattatat gctgtcgaac cagtaggcgt
    26461 agaaatcgtc aatccatctc ctctaaaact ttcaaacagc tcgtttttga tgtatacatc
    26521 ggctaccatc gtacgattgg cccgtttaat cgttgattca ttcagtgcta aaaaatgctg
    26581 gtccggtgtt tcatccaaat aactgatccg aacatccagc aagggataac taacactttg
    26641 ttcccggttc gttcgtaagc tgtctactag ttcttccagc tcgtaatccc gccaatccgt
    26701 gtaaaaccct aaatgacctg tatgcacgcc caagaaacgc acatcattca gacaatgact
    26761 gtagtgatgg aacgccgaca ataatgtgcc gtctccgccg actgagatga ccaattctgg
    26821 ctcatgatca tctcgttgaa tccctgcttg ttctaaaagt gcgttcaaac gttctgtcac
    26881 ttctatagtc tttgcttctt tattatgaac gatcgctact ttcatcccct gcctcctctc
    26941 tttcgctttc tttcttcaaa agcgttcgat agtagacatc atctgatttt cctcttccat
    27001 gggagaataa ccgctgtgct tcttggattt cttcacggat actggacatc tcttcatcca
    27061 actgataggc agcttctgcc gcacgctgaa gtcgttcact catttcttct ggaaaaacgc
    27121 cttgatattt ataattcagc gaatgctcaa tcgttgccca gaaattcatc gcaagagtcc
    27181 gaatctggat ttccgccaaa attttcttct caccgctgat cagctgtaca ggatactcga
    27241 tcaccagatg ataagaacgg tagccacttg cttttttatt tgtgatgtaa tctcgttctt
    27301 ggagaacttt catgtctttt cttttacgga tgatctcaac aacttgatgg atatcttcta
    27361 caaactgaca catgatcctt aacccggcaa tatcagacat ttcttcttct aaacgtccca
    27421 ttggaatatg ccgtaattgt gattttgtta caatactatc gactggcttg actctcccag
    27481 tgacaaattc aatcgggaca tgtcgatttt gttcacggaa ctgtttgcgg atcccccgca
    27541 gtttcacttt caattctgat acagtttgtt catatggtgc taaaaatgct tcccaatctc
    27601 gttccatagc tgatacctct acttcattaa cattcattgt ttattctacc atatatgcaa
    27661 agttttttcg tactcttgat ttatgcattg accaaaaaaa gaaaattagg caaaatagac
    27721 aaaaaggagt cgatcaaatg acacaaatgc ttgaaatcga atataaatct atgctgacaa
    27781 agcaagagta cgagatattc atcgctcact atcaattaac agataaagat ttccgtgtac
    27841 agactaatat ttatttcgat accgttgatg aacagctgaa gaaacaaaat tgtggcctac
    27901 ggatccgttt cgttggtcat caagctgaat atacattaaa aacacctgct gaaaaagggc
    27961 gtctcgaaac aacggatacc tttataacag aagaagcaga agcatttatc ttcgaaaaac
    28021 gtctgcctcg ttcaggcgca gtttttcaaa aactttctga tttgaatatc gatcctgctt
    28081 cattaatcca aaccggaaag cttacaacaa aacgagctga attccctatt gaagaaggac
    28141 ttttagctat tgatgaaagc tggggagaca atctacacga ttttgaactg gaactagagg
    28201 taggggatgc atctgtggga aaaattgctt ttataaattt tttaaaacgt ttttctttac
    28261 cttatcgtcc tgctaagaac aagatccaac gtatgcttga agctaaaaat tagaaacctt
    28321 tcattttcac aaggcacttg agttcatttt tttacttttt tctgttaagt tataggatgt
    28381 gaaagaatct agtcactagt tgtatagaat ctattttggg ggagattcat atgatcgaaa
    28441 tctatttatt tgttaatcct ttaggggaat actgtttcga aatggaacaa cagttattac
    28501 aatttatcga agaagagtac gggacaccat caaaagaaaa gatgcaattt cgctttcttc
    28561 ctttggtcaa tttgcagaca attggggatg tcatgcaaca aaaaggaatt tctcagaaca
    28621 atcttgagga acgtaatcac ttattctcaa tgacttattc tgcagcattg gactgcaaag
    28681 cggcgcagtt tcaaggaaag aaaaaaggcc gccgtttttt gatcaaattg caagaagcag
    28741 tcggctgtaa tgacatacca tattctcaag aacttgctga atccatattt gaagaaatcg
    28801 gcggcgattt gaatatgttt aaagaagatc gtcagtctga cttcgttaaa gaagtattct
    28861 tttctgatca aaaaattgct cgggaaatgg gcatcacaaa acacccttct gcagtcgttt
    28921 acaattatgc ctgcgaccgt gattttggtg ttttagtaga agtaaaaggt acaagtagcc
    28981 tttggagaat cacagagctg tgtaaaacaa cagataactc atatcaagtt tttaatggtg
    29041 aacatttaca tacaaatgat tctcagttcc attcaagaac tagccattta cacttgcttt
    29101 ctaattaaaa a
//
LOCUS       DACCXO010000025        27861 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.026, whole genome shotgun sequence.
ACCESSION   DACCXO010000025 DACCXO010000000
VERSION     DACCXO010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.026"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(398..1342)
                     /locus_tag="IVY70_001042"
     CDS             complement(398..1342)
                     /locus_tag="IVY70_001042"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP7958186.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1638..2582)
                     /locus_tag="IVY70_001043"
     CDS             complement(1638..2582)
                     /locus_tag="IVY70_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7958187.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2803..4017
                     /locus_tag="IVY70_001044"
     CDS             2803..4017
                     /locus_tag="IVY70_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7958188.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4039..6054
                     /locus_tag="IVY70_001045"
     CDS             4039..6054
                     /locus_tag="IVY70_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP7958189.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6137..7510)
                     /locus_tag="IVY70_001046"
     CDS             complement(6137..7510)
                     /locus_tag="IVY70_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP7958190.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7536..7820)
                     /locus_tag="IVY70_001047"
     CDS             complement(7536..7820)
                     /locus_tag="IVY70_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7958191.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7822..9837)
                     /locus_tag="IVY70_001048"
     CDS             complement(7822..9837)
                     /locus_tag="IVY70_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7958192.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10016..10321)
                     /locus_tag="IVY70_001049"
     CDS             complement(10016..10321)
                     /locus_tag="IVY70_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP7958193.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10410..11441)
                     /locus_tag="IVY70_001050"
     CDS             complement(10410..11441)
                     /locus_tag="IVY70_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP7958194.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11648..12523
                     /locus_tag="IVY70_001051"
     CDS             11648..12523
                     /locus_tag="IVY70_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7958195.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12675..13517)
                     /locus_tag="IVY70_001052"
     CDS             complement(12675..13517)
                     /locus_tag="IVY70_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7958196.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13609..15486)
                     /locus_tag="IVY70_001053"
     CDS             complement(13609..15486)
                     /locus_tag="IVY70_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7958197.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15483..16904)
                     /locus_tag="IVY70_001054"
     CDS             complement(15483..16904)
                     /locus_tag="IVY70_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7958198.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16907..20296)
                     /locus_tag="IVY70_001055"
                     /pseudo
     CDS             complement(16907..20296)
                     /locus_tag="IVY70_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
     gene            20553..21944
                     /locus_tag="IVY70_001056"
     CDS             20553..21944
                     /locus_tag="IVY70_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7958199.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22017..22664)
                     /locus_tag="IVY70_001057"
     CDS             complement(22017..22664)
                     /locus_tag="IVY70_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7958200.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22672..23358)
                     /locus_tag="IVY70_001058"
     CDS             complement(22672..23358)
                     /locus_tag="IVY70_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7958201.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23364..24437)
                     /locus_tag="IVY70_001059"
     CDS             complement(23364..24437)
                     /locus_tag="IVY70_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP7958202.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24659..25333
                     /locus_tag="IVY70_001060"
     CDS             24659..25333
                     /locus_tag="IVY70_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7958203.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25338..26678
                     /locus_tag="IVY70_001061"
     CDS             25338..26678
                     /locus_tag="IVY70_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7958204.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26827..27813)
                     /locus_tag="IVY70_001062"
     CDS             complement(26827..27813)
                     /locus_tag="IVY70_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP7958205.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8124 a         5261 c         5048 g         9428 t
ORIGIN      
        1 ttttttatca aaaatcgtca tttttccatc aaaatctgct tatgcaaaat tataaaaaaa
       61 ttttgcagtt tttcttactt tttttgtcgt ttaattgaat gataaaaacc agtttcttta
      121 ttcagttaat caaatatgaa cgaattagat atgaatcatt gtatttgccg ttgcggaagg
      181 attttttaat taaccacaga taaacattga taacgattcg cttattgtaa tcaacagatt
      241 aaatttcata tgttcatatg tacggataca ttttcggacc gattaatttt aaccattctg
      301 tttatttgct tacctattag atatcaataa aaaactgctt aacagctatt tcagctgtta
      361 agcagtttta ggatttttag ttcatattca ttgtttctta taatacagtg ttcaatactt
      421 ctcttaaagc acttgttgat ttgctgaaca ttgctttttc ttcttcgttg attgataatt
      481 caacaacttc acgaacacca ttttcgtcaa cgattgatgg cacacctgta aagatatctt
      541 tttcgtcgta ttcaccagtt aaataagcag atacaggcaa tacagcttgt tcgttattca
      601 agatagcttt tacgatacgt gcagtactca taccaatacc atagtaagtt gcttttttac
      661 gatcaatgat ctcataagca gcattacgga ctttatcagc aatcacatct aattcatctt
      721 gggcaatacg atggtctttt tctacaattt caaacactgg tttaccaccg acagttgtat
      781 gtgaccatgc agcaacttct gaatcaccat gttcacctaa gatatagccg tggacgctgc
      841 gtgggtcaac ttttaatttc aatgcgattt ctttacggaa acgagttgta tccaatgttg
      901 ttccagtacc gataacacgt gatgttggca gaccagattc gttccaagcg atatatgtca
      961 aaatatctac tgggtttgaa gcaacaacga taataccgtc gaatcctgat cccatgattt
     1021 cttttacgat ttggcgcatg attgacgcat ttgttttaac taaatctaaa cgagattgac
     1081 caggtttttg gttgatacca gctgtgataa caacgatatc agcatcttta cattcttcat
     1141 agccacctga ccatacagcc acattttcat cgccccaagc cataccatct aaaaggtcca
     1201 atgcttcccc ttctgctttt tcttgattca catcgatcaa tactaattca ttagagattc
     1261 cttggttgat cattgcataa gcgatacttg ttccaacaaa acctgtaccg acgattacta
     1321 ctttacgact tgtttttttc atgaataaac agctcctttt cataactatt tgttactttc
     1381 atgaaaagta tagtcttgtt tattcatttt atcaacatgt taattataat agttgttatt
     1441 ataatttttg atggaaacga attcctgaat acgcataaca gataaatgac aaatgtttac
     1501 acctatatcc cctgataata caaccaaaag tttagaactg aaaataaatc caaaaaaatt
     1561 cgctgcaacg atataataag acagcaatcg cacctaaaac aattttcctt catatgcttt
     1621 agtgaaagat tttcttctta atgtttattt ctccattcat tgggagtaca tgttgaatac
     1681 ttcttgaata atttgaataa agaagtcgtt tgtttatatc cgatacgatt agcgatttct
     1741 tccaacgaaa aactcgtttc ttgaatcagt tccaacgcaa agtcgaattt tttctttaga
     1801 accagttctt gaaaggaagc atcaaaatac attttcaatt tattactaag atagaccgga
     1861 ttgtagccaa aatgagctgc tgtttctttt aacgtacaag aagtaaaatg cttatcgata
     1921 taatctaata ctctaagcat ctcattttct gcgcatccat ctgctgatga aaagctttgt
     1981 tgttctaata aatgagtcaa ttcgatcagt aatagtgatg tatagagatt taacgaacgg
     2041 atcttatgac tgtatgtatc aaaataaatc aacagcatat tttcaatcaa atgcgtcaaa
     2101 cgacttgttt ttccgccgcg aaaatgaatg aattgtgtat gttccccttt ttcttttgat
     2161 gcatttatca aaaaagaaga aagtagatta tttgtgcttg ccaaatgatt caatatctct
     2221 gtagataagg tttcttcttt taataagatg ttgattaaaa tatcctcttg ccctgtgtat
     2281 tcgattcttt gagtagcttc tttatcaatg atcaccacgt cactttcttc catctctatc
     2341 ctttctccat taataatttg taaacatgta ccgctataca tataattgat ttcaacaaaa
     2401 tcatgtgtat gctcgggaac ttccgcatat cgacggtgtt tactgatatg aatatttccc
     2461 tgttgaaata gatcccctcg gtggattttt ggcggtgtct gaagatggta agctaagttt
     2521 aaatcattga tattcattcc ttgttcacgc tgcagagttt cgattgattc ttcgttacgc
     2581 aaaaaaaatg tctaattcag ctagttccaa ttgactccct cctttttgta agcggtttct
     2641 ttcgcttatt agttcacgct gtactgctat tatatgcaaa gctatttttg gtttcataaa
     2701 gactttctat ggttgggaca tgaacttcct gtgattccgc tattgagaga ataaccactt
     2761 catactatat ttttgataag cgcttacaaa aatgaaagga acatgcttat gaataaaaag
     2821 atgatgaaat tttctctgct gtcagtctct cttattttga cttctgctgg tgcagtcacg
     2881 tctgcaattc ccagtatgtc agctacttat gccacgcgtt ctttatctgc tgttgaatta
     2941 ttaacgactg ctccctcttt tatggtcact atttttgttt tgattagtag ttttattgct
     3001 aggaaaatcg gacaaaaaca aactgtgatc atcggattag tgattgcatc gatttgtggt
     3061 atcctccccc tcttctcaca aagttacagt attgtcttag cttcaagagc tggactaggt
     3121 attggctttg gattgatcaa ttctttggca gtcagcatga ttgctgcctt ttatcacgga
     3181 gatgaacgag cctctatgat tggttttcaa agtgcatttc aaggatttgg tgcagcttta
     3241 atgacatttg ttgcaggaca gctagtacag ttcggttggc aatatacctt tttagtctac
     3301 gccatcagct tacctattct tgtcttgttt gttttgtttg ttcctacacc tgaaacaact
     3361 gaacttcaat cgcatggcac agccccaaca agacaagtaa tcaaaacgaa tcctaaaatg
     3421 attagttatg gtatttttct cttgctggtc atcgtggtat ataatgctgt atcgattaaa
     3481 ttagccacag tcctgacgac ttattctcta ggaaacgaaa ctaatgcagc gaatatctta
     3541 acaatcatgc aattggcaag tatggctgcc ggagtttgtt ttggctggct tcagacaaaa
     3601 gcacatcgct atatgcttac actttcttta ctactgatgg gaatcggctt tgtattgatt
     3661 gccactgtga taaacctgta tgtgattggg tttggtgcga tcctaacagg aatttctttt
     3721 tcgttattca ttccttactt attcaaccaa gtaagtatcc aagcgcccaa gtacgcggag
     3781 agtttcaata cttcaatctt attggttggc gctaatttag gtactttcat tgcaccatat
     3841 ggtttgctct ttctctcttt tgcttctctt gggagtaaaa cacttgctgt tttcgtcaat
     3901 ggcagtatct tgctgttgat tagcgcgctt attagccttc tagtagtaca caggacgaat
     3961 agaaagacag tgataagagt agatgaagaa ataaaagaac aattgacaac aaaataaatt
     4021 tgatggaggt cgtgtaaaat gacaaatgaa aaacataaag atagatggct ttggtatcct
     4081 ggagactttg agatccgcca tggactgcta caaaatttcc aacgggaaga acgaggtttt
     4141 gactggccag cgtactggta catggatgat tgccatcgga acgtcaagtt taagagatat
     4201 tactttcttg atcaaccttc catgtttaaa gtgacgatcc aaggagttgg gtatgtagaa
     4261 attaatggac aaaaacatcc atgtggaaaa tggctgactt gtccagctgg taaagcaaaa
     4321 attcgtatct ttgtaggaca tacatcaggg ctaccagcga tgtttatcga gggagacgaa
     4381 gtaaaatcag atattggctg gacagcaagt aattttattg aagaatatcc agctggctgg
     4441 agtccgcttt atgtcgatat cgcaaaagat ccaaatcaaa tctattatca aaaagaatgc
     4501 gtccatccag tcactgaaac ggagattggc ggaggaatct tattcgattt tgggcgagca
     4561 gtgaatggct gtgtagaact aatatctctg gatgaccata tcgaatctat cactctatgt
     4621 tatggagagt ccgatgtaga agctatggat atcgattatt gctactataa gcaaatagga
     4681 atgacaagaa aaatgaaagc tcgtaaacgc gcttttcgct atttgtttat accagaggtt
     4741 tctactggaa tggttcaact tcaagttttt catgaatttt tatctctttc gaaacaagct
     4801 gctttttcaa gcgattcttc tttactgaat cagatctggt cagtatcaga agaagccttc
     4861 aagttatgta gtgatttgtt ttttatcgac ggtatcaaac gagatcgctg gatttggtct
     4921 ggagatgcgt atcaatcatt catgatcaat caatatcttt tttttgacga ggaaatcaat
     4981 aaacgaacga tattagcatt aagaggacaa aatgaaatca agcagcatat gaatactatc
     5041 gttgactatt cgatcttgtg gttgattggt atcgaaaacc actatatgat gacgaaagac
     5101 tatgaatttt tacgcatcgt ctaccctaag atgcaatcat taatgagata cttgatggag
     5161 caaacgaatg aattagggtt tatttatggg agagcaaaag attggatatt tattgattgg
     5221 tctgaaatgg ataaagaagg aacgcttgct gcagaacaag tcttattact gaaagcctat
     5281 cattccatga tcacttgcgg agatatctta gctgaaacat atggagagaa cgaaaattat
     5341 catactcatc tctatcaaga aaaatatcaa ctattgaaac aaaacctatt tgactacttt
     5401 tgggatgaag aacttggcgc ttttatcgat tgctatgaat ctgggagaag aaatgtgacg
     5461 cgtcatgcga atattttcgc tgttttattc gattttgcta acaaagacca acagcaatcg
     5521 atcttaaaga atgtcttatt gaatgacact atcacacaga ttactactcc atatttcaaa
     5581 ttctatgaac aagatgcttt atgcaaactc ggacagacga atatcgtcta ccaaacgatt
     5641 cttgattatt ggggagggat gttatctcat gatgcggtaa ctttctggga agaatacgac
     5701 cctactcaaa caggaaatga aaagtacagt atgtatggag atccttatgg aaaaagctta
     5761 tgccatgcat ggggagcaag cccaatctat cttttaggac gctattttat cggtttgtat
     5821 ccaacaaagc caggttatga agaatttatg atacaaccta atctagattc atttgaaaca
     5881 ctgaattgta cgctacctat caaagatgga aagatttcgc ttgaaaaaac aaaagataag
     5941 atcgtgatta cttctgatag aacaggcgga acagtgaaaa tgaatggtaa aagttatcct
     6001 cttctagcaa aacagccact tgtcttaaca agctattcaa aaataaatgc ataaaacaaa
     6061 aaactgcttg cgacatatca gaagacttta aatcttcttc tgatattgtc caagcaattt
     6121 ttattttgaa gtttgcttaa ttctgttctt tagaaacaat gtttgttctt tttccggcaa
     6181 aaagaaagct agataatacg acaagtaaaa agaaagcgat gattccaact gtcaaggcta
     6241 tttgaccgta ttctcctagt ttcccaagta caagtcctgg tactagataa tcagtatcgg
     6301 caaatgtaga agaagcgatt ttaagatctc ctaatactgg aaggataaat aatggcagcc
     6361 aactaatcaa taagctattg aaagcagaac ctaataccgc ccctcttcta ccgccagtcg
     6421 agttgccgaa gacaccagaa gcagctccgc agaagaaatg tcctactacc ccaggaatga
     6481 taactgtagt ttgcaagcct atcatagcaa acatggatag tgttcctaca aagaagctaa
     6541 caaaaaagcc aattaataca gcattaggtg catatggaaa aacgatagga acatctaatg
     6601 ctggtttaga atcgggtacg agttttttag caatcccttg gaatgctgga acgatttctg
     6661 ccaacaccat tcgaacccct tgaaggacga taacaaaccc agctgtgaat gttccagctt
     6721 ggatcagtgc aaagattaca gcgttgtctc cattgcttag ttctctttca acaaatccag
     6781 gaccagctaa caaagcaagt gcgacatata cgatcgacat caagagcatg atcgatacag
     6841 tagaatctcg caaaaagctg aaacttttag gaatctttat gtcctctgta gaacggtctt
     6901 tgtttccaaa aaactttccg atccatccac tagaagcata accgatattt ccggtatgcc
     6961 ctaacgcaac ttgattgttt ccagtgatgt tcttcataaa cggttgacaa agagcaggcg
     7021 taatagttaa tagtgttcct tcgaagaccc cgcctaataa gatcatccac aaatttgcag
     7081 taaaccccgc gcttactaaa atgactgctg ttagacaaga aacataaagc attgcttgac
     7141 ctgtaagaaa aatgaattta tatcttgtga atctagcaag taaaatattc acgatcatcc
     7201 ctactagcat gatcaaagca gtaggtgtac cgtattgttg caaagcaatc gccactactg
     7261 cttcattgct aggaactact ccttgagtat gcagagctgt ttggaacatc gaagcaaaag
     7321 gggttagtga attactgatt aaaccagcac cgccgatcag tactaggaat ccgacaaatg
     7381 ttttgatagt tccttggatc acttctgaaa atttcttctt ctgaagaacg agaccaagca
     7441 gtgcaatcaa agaaacgagg atggagggct gactgacgat tgacacaaac aaattcaaca
     7501 atttatccat tgatagttcc tcccttgaat catttttatt cttttgacaa gatcttcgtt
     7561 actttttctt ttacttcttc gctgtcaatg atcgattgca aaggtacgag cttttcatca
     7621 ggtatagaag ttagcgtatc tttcaaagaa tgctcaatga agaaataatc agccatatcg
     7681 ctagatacac ttccaacatc cgtatgactt acttcaaact cgtccacatc tgctccaaga
     7741 tctgcaagtg cttgtttgat gttcatctct accataaaac ttgttcctaa tccactttga
     7801 catactgcaa aaattttcat atcattcgtc tccttttctt atgatcgcta atatttcttc
     7861 ttttgttgat gcgtgtaata aatcttgaag attttgttta ttagaaagta gctttgttaa
     7921 atttgccaaa gcccgaagat gtgcctcatt atcgatagca gctaaacaga tgaacaaact
     7981 aatgggatgg gctggattat caagcagatc cacaggttgt tgaagtttca gcaaagacat
     8041 ccctacttcg tttacgccat cttctggacg agcatgaggt aatgcgatgt gatcgccaat
     8101 atggataaac gcaccatact gctccacgcg ctcgatcata gctttgatat aactttcttc
     8161 tattttattt tctgataata gcggctgtgc ggcaaatgtg attgcttctt gccagttttt
     8221 tggttgatcc gataattgga tcatttcttc tgttaataat tcagatagca taggacgttg
     8281 atcctccttc ttttctaaaa gtttgttcat tttacgattt aatacttgat agagcttttc
     8341 ttcgtctacg ccttctttta attcgatata aggcagtagc gccgacaaca attcttgggc
     8401 agaaggcatt gaaaatcctg gtatcagcca atctctttgt acttgattga tcagctgatt
     8461 tttttccaag tcagtcatga ttggatgcag aacatagaga gactttttct tatcagtaag
     8521 tgtgagaggt acagtagaaa aaataatatc gtaagaagtt tcgtcaacct cgtgtaagcg
     8581 gtcagctgaa ttagtctctg taaaaaagat catcgggaat aattgctgaa gttcactttt
     8641 taagatcaat gaagagctaa tgccacttgg acagacgatc acggctctta tttttctatt
     8701 tctttcttct gcgtccgctt ttctgatctc tccgccaaat agtatagtga aaaaaccaat
     8761 ctcttcttca gggatgctct tccctattcg tctttctaac ggaagtaatg cttttcttgt
     8821 catttcgtag atggaagcat attgatgctt aatttgatcg atcatcacat taggtaaata
     8881 aaaagaatag gaaatacgaa aataggcagg gaccaagtga taaaagacat tcatcaataa
     8941 ttcacgaaac gaagcaaatt ctacggccat caggaattcc atgcgatgga tgacttctga
     9001 agaacattcc agcaaaaaat caagtgcagg ctcctggaca tctccttcaa cgatcgatgc
     9061 taagcaaaga gagaaaaata taatctccga attttgatgt tcggaatagc caaggccagt
     9121 caatacttgt cgactaatgt cagtgactgc aagagaagcc aacagttcag catcttttac
     9181 cagttccatg gatgtttttc caattcgaat ggttgtcaaa ctcaaataat aagctaattc
     9241 atccattcga tttggtgcga atcgaagatc tgatagtgaa atcgttttat ctagcaaatc
     9301 tctaactttg gcgtaataat caatggaaag ggttgcggtt aaatacgtga gcccaaatct
     9361 tccagcaagt gactgcatca aacgagcagt gcgatttttt gcttctcttc ttaatattgc
     9421 ttctgaccct agcagcctaa aaccagtttt cctagtatac gacagctgac aatcttccac
     9481 ttctaattca tttcttatct ttttgatatc tgacaggact gtccctttag aaacctgaag
     9541 aaaatcttga aaatgaaaaa cagaaacaga cgttgcagtc gaataaacca tcaaataaag
     9601 aagatctctt cgctcttgtt cggtatacac agcttcaaat cgagtaatat ccaaaaattt
     9661 ttctgcccag tcgattgctc ggaatttctt tatacggata cgggtatcat ccacatattc
     9721 ggcaaaagga gttaattgtt tgatcgcctc gcttgtattt acttcgatga tctctgctgc
     9781 tttttctaga ctgatttctc ttggtgtttt caggagataa ctaattttgt agttcacagc
     9841 gctgttcacc tcctacttat attctagtat ggtagcgctt ttaaatcgag gggtgtttgg
     9901 tacaacttac agttggattt tttttacgac tgtaccgaac gatttctatc gaatggatac
     9961 agaaaaaaag caggtgacca aatgtctcct gcttttatga attgatttcg tccgatcaat
    10021 gatgatcatg tttatttttt gaggtcagtt cttcaaattt ttctttgaca tcttcaaccg
    10081 ctttttcgat atctttttca aagtgattgg gctcattagg ctttgtttcc tcttctacag
    10141 aatttgaacc agataaagct gtttctttat tttctgtttc accagagttt gtgccagcta
    10201 atgattcttc tttgtcttct gtttcaccag aattagttcc tgctaaagcc acttcttctt
    10261 ctgttttagg ggccattgaa cctcttaatc gtttgtgttt ttcaccgtca taatttgtca
    10321 tgttggttcc tcctagtagt tctatacttt ttcatcgtac aataagacag tttatgcgtc
    10381 aaaaaacaaa cccgattctt acaaatatgt catatttttc cttctgattc ttgaatgctt
    10441 ttgattcctt cttccaaaat tttcatttgt aagattgttt cttcatcttt gatgactttt
    10501 tctgcgccat taactaatgt ctcgtaaatc ccttcgtaga cacggctgta atcaccaacc
    10561 tcggaaatta ctttttcttc atgataactc ccttgttcat ctatataggt caatacacca
    10621 taatgttcgg gcaaatcgat accaaagtct tcatgatcag gcatgtaaaa cattttcaga
    10681 tgttcttctt gtcgatcttt tgtttgtttc acgaacatcc ctttttttcc gtaaacgacg
    10741 aaacttggac gttctttgat gcggaaataa ctggatttta tcgaaatttt catatttcca
    10801 tagaaaaaat ccagatcgaa gtaatcattc attcgtcctt tccctaaaag ttgacgtaca
    10861 tcatagctga tatcatctgg ttcgccgaaa taagaaagaa cttgatctac tgtatgacaa
    10921 gcatgtccat ataaatagct gttcgcagta gaaaattcat gcgtattttc tggtacttcc
    10981 ggtctgaaat aatcataatg catttctact tctaaaatat cccctaattt tccgctttcg
    11041 attacttttt ggactgtcaa gaagtcagaa tcgaaccgtc tgttttggta acattggata
    11101 aatagacctt tttcttttgc taatgcaaat aactcttctg cttcctttga cgtttctgca
    11161 aaaggtttct ctactagtac attcttacca tgttcgagtg ccatttttcc atattcataa
    11221 tgaaattgac taggtgtagt gatgacaact aattggatct caggatcatc ccaaagatca
    11281 gacagctgat cagtatattc gatgtcttcg aatgttttcc acactggttc agatggggaa
    11341 aaaatttttt tgatattgat tttgttcgta agttttcttg aaaaaggcaa atgatagcga
    11401 tttgtactct ttccattgcc aagataagcg attgtaagca tgagtggtgc ctccttttat
    11461 tgatgttttc agtatatcct agaaaagcgc agaaagtatt tataatagct attctggtaa
    11521 aaaagttcag aaaaacactt gttatcattc ggtttggtga aattaatgtt caaagtaagg
    11581 ataaaaatca aaaagatagt agcggaacca agtgtttatc agtaagatag aatttggagg
    11641 tcagatcatg cttattgaag aaaaactaac aaaacaagag ttgtttacca cgacagagaa
    11701 acggattgtc gattacatcc gtaggaatat cgaagctgca gtctacatga cgattgaaga
    11761 attagcaaaa gctacgtata cttcccattc agctatcatt cgtttatgca aaaaaatggg
    11821 attcagcgga tttaaagaat tccgtttcga gctggggcgg gaagttcatc aattgattgc
    11881 tcagttcaat caaacgattg atcctaattt tcctttttca agtgaagata gcactcagca
    11941 aattgcaaag aaaatggcgg atttatcgat ccagtcaata aaaaaagcac aattgcagat
    12001 tgaacatgaa gatttagatc aaatcggtaa agtattgaac aaggcacagc gaatctttct
    12061 ttttgcaaaa ggtgattctc aaatcacggc aaggaaattc cagaataaaa tggtcaagtt
    12121 gaataagttt ttgatcatgg ctgaagaata cagtgattct tcttggaatg cagccaattt
    12181 gactagcgag gattgcgcca tttttattag ctacagcggt cgcatccatc attatgaacg
    12241 aataatgacc tatttgaagc atgtagaggc ccctaccttg ctgattaccg gaaatcagca
    12301 ttcagaaatg gccaaacagg ctgatatgtg tcttgttata tcgcaggagg aatatgattt
    12361 tgctaaagta gcgactttct cctctcaaat tgccttcgat tacgtattaa atacgttatt
    12421 ctcagtgata tattctcaaa attttgaaga gaatatccgc aacttgaaaa acaaacaaga
    12481 attgatgcaa aaaggccttc ttaaagaata tcggaaaact taagaaaaga tcctcattta
    12541 gtaataagaa agatagtgtt tgataggctc tctttgatat catcataaaa acaggctgaa
    12601 gttttctttt tcaaacctca gcctgtttgt ttacggaacg attcgaatag atatattata
    12661 gaaatgagga atagctaact tctgtttctt attttgaacg attgacgaaa aagatatata
    12721 agaaggagga aaatcagtat acatgcagct aacaatccat acaggaaaaa tcgtttgttc
    12781 tcggatactt tctgtacttc tttttgaact tcttttgcat gataaggaac ccggtgccct
    12841 ctgacaagta agcgatgact gttgaccata taaggtgtac aagttaggag tgtcacgaaa
    12901 tcctgccctt tttcgattct cagatctttt gtatcagtcg gttctaccgt cttgatctga
    12961 tcgacttgat aagcgagtgt ccgtccattg atttcgatat agaaagaatc acctaaagtc
    13021 agtttcggta agtctgtgaa caactttgct tgaggcagtc ctcgatggct ggaaataacg
    13081 gcatgtgtat tttctccccc cgtaggataa gacgttcctt ccaataatga ggcacctttt
    13141 tctagcagca cagtattcgt ttcatcaaaa attggcaaat ggacattgat ctcagggatc
    13201 gtcaaaatac cgatcgtatg tttctcaaaa taggttctat cctttttcgc aggttctttt
    13261 ttcttagtaa agggatctgc acctggattt ccaccttctt ttgctaattg ctggtttttc
    13321 tttgtcatgt tttcttggat tttagccatg acttcttcat tttctttgac cgcttgttgc
    13381 tgataatgag agatgatctg ctgatccaga taattattca gcgcgtcgct aacaaatgga
    13441 taagctagta tccctattcc tgagagaagc aagaaaagca tcagcagatc tagaagtttc
    13501 cttttctttg gtttcatttg tgttctcctt gtgatgactg tttgataata aaagaaaaag
    13561 gagcatttgc gtaatccgct gctccttttt ccaattcttg caaaaagact aaatctgact
    13621 gtgcttgcgt ctagcaaagt acagtccagc aatcagtaga aggactactc ctgcaccgat
    13681 atacacatag attcccttac cgcctgttga aggaagtgta cctttgtgtt tatttggtat
    13741 tttttcagga gaaatcaact gtcctgctgt tacataagat tgttcatcga tagtaaatgc
    13801 tacacggttt gttaatggaa catatttttc tggcgctttc gtttcttcca gatagtacgt
    13861 gccatatttt agacctgtca catcgattaa accgttactt gtggttgtaa aaaccgttgc
    13921 tgattctttc gccgataccc agctgacggc ttttgtggat gggtcgatcg ataaatattt
    13981 cgctgtgtca ctatcttgat cacgaacgac gaattctgct ccagcaagtg tttggtctga
    14041 tgtaacgtca ccatctactt taacaaatcg tttgccccca gtaacgacat cgactgacgg
    14101 tggtgtttga tcatttgtat gcccgttatc gacatttgct tggttagaaa atcctttggt
    14161 tggatctgct ttttcgttca aatgcatata gtaaacgaat ttcaatgtac cgccaggagt
    14221 taatgaagga atataattcg gatcaactga aaccgtgaat ccgtctgttt gctcagtgac
    14281 agaataattt actgggtcga tttctttatt tccatcatat aaggcataag catacgttcc
    14341 tgaagaatca ttatcaaatg ttaaagcagc gtcatgagta tcgataagtt tgaatgttgt
    14401 gtacttgttt tgcgttcctt ctttatcagc gatacctaat gggatattga cagaaatctc
    14461 atattgaatt ttttcaccga ttgcgacatc ttttccattc aattgaggtg ttgttttatc
    14521 aactttagac gtatcatttt tgatggtctt ttctactggt gtttggctat ttgccaggat
    14581 ctcaaaaggc gtgattgttt gcttttcaat catttccgca ttatcaggag cttttacttc
    14641 ttctaaatta tattttccaa cttctaaatg attaacgatc aactggcctt tttcaataga
    14701 tgcctcggca aagtcattgt tgccgatgtc ataagaatga ccagtaatga aatgtttggc
    14761 tttggtttga tcagttgtcc aagtgtacaa tccatctgtg acactttgga tgtatttttt
    14821 gacgcttggt gttccttctt ctttagaaat aataaattct gctccattta gtgcttcgtt
    14881 ttcggcagta ccgatttttg taactttcaa cgttccatca ttaccgactg tatttttagg
    14941 gtagagatgg atagtatcta gttcttctgt cccgtattta taagagccat ctgcttgttt
    15001 gatcatctca tatacaggga aagctaaaac catgtttgcg gcagctgaca ctccgtcttt
    15061 tggttcttct ttgatcgtgt agactgcatc tttcccattt tgttttttag gtaaagataa
    15121 agtgacattt ccatcagcat ctgtcgttcc tgaagcaact ggtgtaccag gagtcaaaga
    15181 ctggactgct tgttttgctg catccacgga cgctccttta tctcgttgcg cataaaattc
    15241 ttgagtgacg ttataaactg aaaatgaaat atcggctaat ccttggtatt gatcgaattc
    15301 gctcatttct ttcccgctgt tttggattaa aggatcgggt aaatcagtca ttttcttttt
    15361 atgaaggatg acttgagctg ctgtctcctc tgcaaacact ttttttgcgc cgaagctgtt
    15421 tgtgagtaat ggtaaaataa ggaaaaggac tcctaacata acgtttattt ttttatgatt
    15481 tttcattttt tcactcctgt tctttttttg ataaacaacc agattgcaaa gcttgcgaat
    15541 accataccaa caacgcctaa aagtgtattt ttcgtttcgt ttgtttttgg aagaaatgga
    15601 cggctatttt cttttttatt gactactttc aatatactgt ccgcctcttt atcctcagaa
    15661 agaaaagttg aaatggtaaa cggaatttct gtttcactta aaatataacc agaaggtgct
    15721 tcgatctctt ttaatcgata acttcctgtt ttcaacccgg aaattgaaaa agcgccattt
    15781 ttatcggaaa tcaggtgaag cgcatctgat tcgttctttg tccacttgta tccgttggct
    15841 gtttgctgga ggtaatttcc tgctttgttt ttaataagga atactgcgcc agctaatcgt
    15901 ttatctggtt tatttgtctc gattttgaca aattgcttac tccctgtata tacagagact
    15961 ttctttgtac tgacaccaaa accaaaatcc agtgttccgt tgttgttgat ttccttgttc
    16021 gcctgtgctg tactgcttag ctgcatctga taagaaatgg tcagtttttt tccagcatat
    16081 ttttccaacg aagggatcga aaataaaaca tcaaatccgt gaggtttctt gtgtatgaca
    16141 aagccttctg tcagcttttc tccattcgat gaaatcgtta aactattagg taaaaacgtt
    16201 aatgcttcat ccgcactatc tgacaattcg aacttttgat agtcaaggat atttacagga
    16261 attgtcgtat gcaaggaata agtgattttt tcaccaatcg taaaatcatt tctaggctct
    16321 tctaatacct tctcaaaagg tggatcaggg tagtcgtttt cttcattttt aggatacaaa
    16381 tggatagttg aaagtttttg attgttttgt ccatgtacag gaagaacaac taccatattc
    16441 tctgcctttt ctttgacgac ttctggtact ttggattcaa tgaaaagata cgctttatct
    16501 cttttttctt gatctttagc ggccagagaa aaagaagcga taccatcttc gttgttaaga
    16561 gttgtagttg ttgcttctgc ggtaaacata ccggaagacg caccgttttt tgcgatctct
    16621 gcttgtgctt cttctaccgt tttgcccttt tcccgtagat ggtaaaaaga atctgtgaca
    16681 tcataaactt ggaatgtgac accatttaat cctcgatacg tttgtaataa agctttttct
    16741 tgtccgtcat ttggatgatt tttcggcagt tgcccgtttg ggaaaagcaa tttgtgtaaa
    16801 gtaatctgaa ccatttctgt ttctgtttct gttgctacct gagtaaaagc tggtttaaat
    16861 agtaacaaga agagacacat actaagccaa ccaagttttt tcatatttag ctgccccctc
    16921 tttctttttt caaaaaaaga taccaaatag aaaacgcaca accaatcatc cctactagat
    16981 agattcctaa acggccaatt cctcccgttt caggtaatgg tacttttgcc tgattcgtga
    17041 tgtctaaaga aatctggttg tttttggctc ctggtgacag aacagattca tgatcttgtc
    17101 catccacttg aattgaccca tcttcgtgta taactatagt aactggctct tttagacctt
    17161 ggtatccttc tggtgtaaaa gtttcggtca gcgtataagt tccaggtttt aaattttcga
    17221 atagaaaggt atctgtttct tgtccatctt ttggcgattc taagtccatt tctggtccct
    17281 gcagtctgaa ttttgctcct tttaatgtct gtccgttgtc ttcttttttg tgtaccgtta
    17341 gatcaaacgc ttttagtcga ttttgatgag tgagcgtcca tacattattt gtcatctcgg
    17401 tattttttcc cgtgtaaatg atttttccat atttatcgat ttggaattca taatttcccg
    17461 gcgaagtatc atatccggca ggaccagctg tttcaacgac tcgatattta ccaggtgtga
    17521 gtgaatcaaa actggcaaga cctttttcat tcgttgtttg gctgtcaatt ggctgataag
    17581 taccatttgc ttcttttttc tgtagggaaa atgctgctcc tttaagattt atctctttgt
    17641 ccgtcccttg catcgcatac ttacgtactg ctacaggaac ttcaacaaaa ggattttcaa
    17701 tagtcaactg gatcgtatca tcggaagtag tgactgtttt tccatcaatg gttaccgtac
    17761 catcagaagc gatcttgatt tcccaagtag tctttttgct taatccatgc ccttctggag
    17821 cttttgtttc tgtcagcgta taggtcattt ctttttgcaa tttgacattt tctggaagag
    17881 aataggttcc gtcgccatga tctgttagta aagtatcaat agaatctcct gttaatttga
    17941 aaacagcgcc aataagatcc ttttcacctg tagaggaatt tttcgttatt ttcaagttta
    18001 acggtgtgaa ttgtttagta tttttccatg tcattgcatc tatttgttga gaatcgtatc
    18061 ctggtacagg taattctttg attgtttggt aactgaatgc ttgaccttga ttattgtatt
    18121 gaggtagtga taatatctct tgataacttt ctccgctatt tgcagataat ttgtcaatgt
    18181 ctgcacgttc ccacgtattt gttgtatctt tagctggttt aatgattcga atatatccgt
    18241 ttttccaagc tgcagcattt gtcgtatgtt cccgttgaat ctcaaaagta acttgatctg
    18301 gacgatcagc tagattgttg tcaaattctt cccataactt tttgatgtga agactgactc
    18361 cgggagcttt agcagatggt ataccgaatt cagctaattc attggtatca atgtttggct
    18421 ggaaagttgt cctgccgttc atttgatacc agaaatttgg atggaagtcc tcattttctg
    18481 tttggattct cacttggtaa tggatttgga tttcttggtc ttttcataaa tagatctgat
    18541 tgctcttgat ggtatttcct tctatggaaa tagatggagt gaccgttgta ggacttgtcc
    18601 ccacactctt gactgaaagt gtaccaggct gataaatgaa tggttctgcg attggatcat
    18661 ttatttgtcc attgcttaca gttgctgaga tctgtacagc ttttttggct aggtattcag
    18721 agatatctgt tgcatgatca gctgattcat agtaaagatc gcctttttca tctgatgaaa
    18781 ccatttgtcg catacgagac tctacttctg cttttgagag accagctgcc ggatcgcttt
    18841 gaagttggat gccaagacca tgtatttcga ttcctcgttc tttgagtgcc atcgcttctc
    18901 cgatggttgc gataaacgta ctgtcgattc ttctggatag attgttttgg tcaggtgcat
    18961 catagattct tgagattaga gaagtatttc ccggccgatc ttgcgtatta gaaaactgag
    19021 ttccgtaata attgctgctt gattgtgcgt gtacccgctg tactttatag gaaaatgttg
    19081 gtacaccatc cgtcagcaaa acgatcactt ttttatgtcc atttggaacg gatagcatgc
    19141 ttcctgcatc tcttagtgct ttttgtgtaa aagtaccacc atttgtccgt gaaggtgtaa
    19201 tggattttac ttgatttttc actgaatcaa atgaacccat ctgtactgca ccgttactgt
    19261 agctatatcc ttcgcttgag tagccgacat atcccatatt gattttgtct gtgataccgc
    19321 tatctgctaa agtatcgaca aaacgatcga caccaatctt tacttcaccg atccgattat
    19381 tgtcgttcat acttcctgac cagtctacga ccaatacgag atcaagaggc gtgatatctt
    19441 tttgtacatt tccacgagca ttcaaataaa catcaaacaa tccgggtgta cttgtttcct
    19501 ttgcatactt tcgtatcgca tagtctgctt gattaccggt tcctccatat tcgatatacg
    19561 aatgggtccg atcattagga tctccattcc aagaatttat accatcccat tgatttgttc
    19621 cgtttttatt gccttgatga tcaagaacat ttgtatttcc tgtaggttgc caactggctt
    19681 taggataagt gccctgatca tctgttgtat attctgtagg aatcgtttga ttagatggca
    19741 cactcacagc tggcaaggat aatgtaggca atgtaaatgg gctgacagaa ttttcgtttt
    19801 cttttgatga ttcttcttct atttcgtctg gctttggtac tgacacatct gtatctttct
    19861 tattttctgt ctcatcaata atgtcatatt ctttattatc gacgatcagc ttgtttgcct
    19921 ggctgtggta aagtgctgga tcgattggaa aactcagatc gatagtttgt ttagtgtttg
    19981 atgagatttc caacgtaatt ttcccgctat ttacctgata agcatagcca tctccttctt
    20041 ttctttccac atcacttaag taattttcga ctagaccaat ttctttttct atcttttcag
    20101 cactggtgtt attcgcattt agcttgatgt tcagtgcttt ttttccagac tgatccgaaa
    20161 tgacttcagc tgaaattgtt tctaaaactg ttggatcatc tgtagtgtct gctattgcag
    20221 ataatggcac aaagtttgat aaactcaata ttatcagcat gaaaataact ttcagtttct
    20281 tccctgtggt cgtcattttt cccctccttt ttcttttttt ctgttttgac gtgaatagtg
    20341 aaaattatta aaataaacag gcgttacata attttattta ttttaatatg ataattatta
    20401 tacgaagggc gctaaaggga atcacaccaa ttttcatacc aattggtttc atttatgaag
    20461 ttgattcctt cgattaatga gcgtttcatt gaaagaaaag ggttgaggga gaaaaacata
    20521 tgatcaggtt gttggaatga aactatagaa agatggaaaa aatcattcga attctgtcca
    20581 tttctgacaa gtggatgact aaaaaagaac ttgccaaacg aattggtagt tcagaatcta
    20641 catttgtaag gtatatcgaa gagatcaaaa tccgctgggg aacggatgta acgattcaat
    20701 cttcacaaaa attggggtat cgtcttgaac atttgaatgt accagcttat ttaaatgttc
    20761 tgatggaaat gtcgcaaagt tctacaaaca gtcgtttatt aaatgagatt attgtcaatc
    20821 cgggtaaaac gattgagtat tattgtgaaa aaatttccat cagccggtct tcttttacac
    20881 gtaagttgaa acaatgcaat caaattttag aaagctattt attaaaagtg atcgttgacc
    20941 aaggttttca gttaactagc cttaaaagtg agctccagtt gcgtatcttt gttacctttt
    21001 tctttttaag ttactacgga agacgcacgt tgccatttcc aatcgaaaaa aagaagatca
    21061 aagaaattat ggtcaagaat aattgtcaga tgaatttgtt tcaggaagaa agcccatatg
    21121 agcaatcttt ctttattatg tattttgttg tgcatctcat gagagaatcc caaggaactc
    21181 catttgaatg gaaagctgga gactcttatg tacagttgat aaatatcgat aaaaaagact
    21241 tcttaatctt gaaaaatctc ttaggaaaat tgaagtacag cagctatata aaagtagtga
    21301 actattttgt caatacgatt ttttcagaat tttcggagaa agaaaaaaat gaaatcagtg
    21361 agcagatacg aaaaaatatg tcctactgtt cgttttcatc cgctgattgc aaagaaaaaa
    21421 acgaagaata tgttgttcaa ttattaacgc aaatttattt tttctcccgt attttcccat
    21481 acgatacgac tcatttgacg atccgtgcaa cttactttgc acagcagatg caacaccgcc
    21541 agatacgtct atatgctgga tttcgtgcag aacttcaatt ttattccact ctactgaatc
    21601 agaatctgat ttattattta tctagctata tcttttggat gcttatcggt aatcctgaaa
    21661 ttcaccatta tacttctgat aaaaaaatac tgattatcag cgacttaagt cttcgacata
    21721 gtcaatatat tgaagaatat atcagtgata ttcttgctgt gcataaaatt cactctgaga
    21781 caactgctat tactgaagat cagctgtcga aatacaatct tctcgagtat gatctgatcg
    21841 ttacgaatca acccattttg aatgcccatg ttcctcatat attgattgat gatagtgtgt
    21901 cattcgcaaa tgaagaagaa ctcatccgct tattcgagtt atgaataatc aaaaaacagc
    21961 tatgtataaa ccaccctgat aaaaatttca ggacaaatac acatagctgt ttctttttat
    22021 tgtattcctt cagcaatcca acgagattta tagcgaaagt cttttaacaa ctcttctttt
    22081 gtccaattat ttgcaatgag atccgtcaaa gtgttcatta cgagggtcat ggaaaagtat
    22141 aggaaactaa ctgcttcttc cgacgattga atggagaaat gattgattct taataattcc
    22201 agccattttt cgttcattgg agaatcagcc gatagttctc tacgtttggt tgtctgctga
    22261 tcatttcgct gggttaacaa gtataacctt tgccaataga tactttcgtg atctaagtcg
    22321 cagcaccact ctaaatattt ttcgatcccc aaaaagaaat gttgtttgtt ttcgctaatg
    22381 aattttaaga tatcttgatg gactatgtga gaactgcttt tgatcgtata caaataagca
    22441 tcttccaaat cttgaaaata tttataaaaa gcaccgcgcg acatccccat cttgtcaacg
    22501 atttctgaga ctttgacttg gctgatgtgc tgtgagcaaa atatctccag taataggtca
    22561 tcgattctct gtttctttgc gttcgataaa ttgaaaaaag tttcttttgg catccacatc
    22621 tctcctttat ctctgattat acgtggatag cccttcattg tcatgaagga ttcattcctt
    22681 ctttttctct tcttctgtca agtacccatc ttccattcga taaacgcgat cactccattg
    22741 aatcatacgg acatcatgag taaccataat agtcgctttt tgtttctcat gagcttcttt
    22801 aactagtaat ttgacgactt catatgcatg atctgtatcc aaactagctg ttggttcgtc
    22861 tgctaagatc aagcttggct cattatataa agcgcgagca attgccactc tctgtctttc
    22921 tccaccagaa aggtctttag gataactatt tttaagtgac tggatatcta aagattgcag
    22981 caaggaatcg atttttgttg aatcttcttt ttttgaagta acttggtcaa ctagttcaaa
    23041 ttgttccttt accgttaaaa aaggaatcaa attagagctt tggagaataa atcctatttc
    23101 tttaaagcgt aaatctgctc gttttttttc tggaagctca gttagtgagt gaccgttgat
    23161 cactatttct ccggaagtcg gtgtttgcaa tcctcctgaa atggtaagga aagtactttt
    23221 acctgaacca gaaggaccaa tgatgctgac gaattctcct tcctttacat aaaaatcaat
    23281 tgctttcaaa gctgagacct ttgtatgacc agaaccaaat gttttggtaa cttttttcat
    23341 cgttaatatt tttttcattt ttattaacct cctattgctg taattgggtc tactttggtt
    23401 atcgtacgta tcgaaaacaa tccgcctagc attgcgacaa tgatcaaaat cccactatat
    23461 agcaaccatt gtgaccaaaa aacagcaaaa ggcatcgcgc ttggtaaaat cagactagtc
    23521 aaataagcaa acaaaagagc aagcagaacg cctaccaccc caacaataaa ggactgtgct
    23581 actaaagatt tagcgatgaa gaaatttttg atcccttgtg ctttcattac tccaaaaata
    23641 gcagtcttct gaagtgtcat cacgtacatg aaaataccga ccacggcagc aaccactaag
    23701 aacaaaaagt atatcatcgc atttaaggtc atattttggg cagaatagcc aggaatactt
    23761 tcaatgaatg tcccaatagt taataactgt agacccttcg atgctttttc tttcgaaatt
    23821 ttagctgaat cctttattac gatcccattg atcggctgtt cttcttcaga agcaaacggc
    23881 tgttgtccat acttcaaagc agtccacgta tctagggacg catagatgac gggagaaact
    23941 gtgtaataag tttcaggaaa gataccgaca atcgtcaaag gatcgtcgta actgccaatt
    24001 ttgatcgtat ctccaatttt atagcctgaa tcagccagat tttgagagat gatgatttca
    24061 ttctttgttt taaattcatg ccctttcgtg agttttggta ataaaaatgc ttctttcgtt
    24121 gtgccgaaaa ctgtaacatt ttctttgttt tttcctttga ttgctccgct gtacaatccg
    24181 attggtgctt tctctcctcc ttcaatgtat ttcagatctc ctcttgttaa ttgtgaagct
    24241 gcaaatactt gatttgcatc ctccgacaaa acaatctctt gtgcgtccca atcatcgatt
    24301 gcttttttaa actcttctga aagtccattc gctagtcctg acaacatgaa tacgacatac
    24361 gcaatcaaga acataatacc gacgatcaag ccatatcgta atttagaata gcgaatttct
    24421 ttgatagcta gaaacatttg tttttcctcc ctatgataga ttgatagtga caaaatgtca
    24481 cttgttgaat agtgtaactc taaagtgaca atttgtcact tgtgttgttt ttttcttatc
    24541 aataaattgt ctaagacatc tttcgataaa accttaacat gagtcataaa aaatttattt
    24601 aagccttatt tatgctttcc atgataaact aaagtgacca aatcaatgag gtgggaaaat
    24661 gattaaacta ttagtagtag aagacgataa agtattatcc aataatataa aagaaatact
    24721 cgctgcaatc ggcgagataa cacaagttta tgatggtagt gaaggactgt tcgagttgga
    24781 aagtggagtt tatgatcttg caatcttgga tttgatgatg ccggaaatga atggatatga
    24841 agtgttagca aaaatacgga aagaaaaaat acagactcca gtattgattt tgactgcaaa
    24901 agatagtttg gaagataaga ttgaaggatt tgaacgaggt gcagatgatt atctaacaaa
    24961 acctttctat cgagaagaac tgatcatgcg cgtgaaagca ttattgaaac gctcgctagg
    25021 tctctttgat gaaaatgttc tgaagtataa ggatatcagt tgtgatttaa aaagcaatga
    25081 agtcttgtat aaagaacaag ttttgcccat acaaggaaaa gagtttgaat tgctcgttta
    25141 ttttttacaa aataagggaa tcattttgac aaaagagcag atatttgacc ggatctgggg
    25201 atttgattct gatacgacga ttacagttat tgaagtttat atgagcaact tacgaaaaca
    25261 tctgcgaagt actggattgg ataaagaaat caaaacctta cgtaatgtcg gttatatact
    25321 gcaaagggtg tgatgcaatg aatggagata ttacacgaaa gcaacgtagt cgcttttttg
    25381 cgacaaatgt agcagcattt gcttttatct tttttctttt gggattcatt actttacaga
    25441 tattaaacca atctgcatac agagaaacag atctgtcatt agagaaaata aattttgatt
    25501 ctcgaacagt acaattagag atacaaagat atcagcaagg cgatcccttt ttaaacccga
    25561 ctcaaacgga acaaaacaga acaaacatgg aacagccatt aatggaacca tcaaatgacc
    25621 gttttaacac tcaagtgatc ttgtggtcta aaaatggtga gatattgaat aaagcagcta
    25681 ttggtgggcg tttcaaccaa atcaaagaat taaaaatgga tacgaaaaat ctgaatacca
    25741 tccaaaatat tgaattaaat gaagacagtg gctcatctgc cctctctttc cgttcgatta
    25801 cgaaaaaagc ggagacgaat caagatggcg ttgcctacat ccaaatttta gaaaatacaa
    25861 atcaaatcaa gaattcatta gcgacttttc gcacgattat gattttgtgt atgattgttt
    25921 tttggattct ctctattggt atcagttatt acttgtctag tctaagcatg cgccctatta
    25981 ttctttcttg gagaagacaa aaagaatttg ttgagaatgc ttctcatgaa ttgcgcacac
    26041 cgttaacgat tattcaaaat agtttggaac atctttttac caagcctaat catactatta
    26101 ttgaagaatc cgaaagcatc gctcaagcat tgtcagagac gaggcgcctc actggattga
    26161 cttcggattt gctgacaatc gctcggaatg attctgatca gcagctgctt tcaaagcaaa
    26221 tgatcaatac acaagaatat atcgaacaac ttgttaaacc atttcaagag atggccatca
    26281 tagatggaaa agagtttatt ctccataacg atgcaacatt agaagtacca attgatgaaa
    26341 aaaaaatcca tcaagttttg gttattttgc tggataatgc attaaaatat acgaatccag
    26401 aagataagat cattttggat tcatctgtta accataaaga atggctgatt cacgtaaaga
    26461 atactggccc ttctatcagt gaagaagatc aagcaaggat ttttgatcga ttttatcggg
    26521 aagatcaatc tcgggcgaaa gagactggcg gatacgggct tggcttggca attgcaaaac
    26581 aggtaatcga acagcatcat ggacagattt ctgtatccaa ttatcttcca caaggagtcg
    26641 attttaaagt ccaattgccg ttgaagccta aaaaataatg cttatcccta aaaaagggct
    26701 gtgacaaaaa tcttgtcaca gccctttttt gttgcgaata aacggtgttc cagaagtagc
    26761 ttcttcggaa ataagccgaa atttctttaa gctgaccact tctgtcacaa cctctttagc
    26821 ttacgcttac gattttttat tgcggtcttc aatgtgtaca tcaccgcttg tgtcaatatc
    26881 caattcttct cggctaacat cttctgtcac tttttcaaca tcttcatgtt cttctttatg
    26941 aatcccaact tcttcacgaa taactgtatg tttgcttaca tctacttgct cttctttgat
    27001 tgggatagta atcgtgtcat cttccaattt ttcgtctgtt ccctgtgaag attgatctgt
    27061 tactggtttg cgttcgatga cgatttcttc acgtttgacc ggtacttcta ccgtttcagt
    27121 atcgttgacc acatgtttgt ggatgtcgac ttctccagtt gtcacatcat gtgtatttac
    27181 atctaatttt tcttctttta gttgaattgt atcatcatct agatttggtt tgcgatcgat
    27241 gtcttcagtc ttgcttgtac ccattgtatc agtctcgcca ggtacgtcca tcggatcagt
    27301 tcctggtgtc atattcaatg gatccgttgt attttgaaag cctgtcgtgc cacctacgtc
    27361 cattggatct gttccagtgc cagtttctaa tccagtcccc attgtgcttg ctgggctaac
    27421 agtatcttgt gtatccattg tatcttctgt tgtttctggc tgacgatagc cttttactgt
    27481 gatgacaaat tttccatcag aaatatctcc tctatattga tataaaggat cattttctgg
    27541 tgtagccgtc tcagaatcat aggtatcaaa agaaaaagta tcttttattt tttcccataa
    27601 agaacggtct tcttcaccat ccatccgatt ttcatctgtt tcaacatcta taccagacaa
    27661 tccgtcaaat cctagtctag atgctgcttc atcagtagta taaataacga tatcatctcg
    27721 ttcataccct tcgttacgca accgttcaat tactgataat gcttcttccc tcgtactata
    27781 gcttccgatg accgttaaat ttttctcttc catgtaaatt cctcctagca aatagattta
    27841 tgattggtat attgttactt a
//
LOCUS       DACCXO010000026        27597 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.027, whole genome shotgun sequence.
ACCESSION   DACCXO010000026 DACCXO010000000
VERSION     DACCXO010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.027"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(287..649)
                     /locus_tag="IVY70_001063"
     CDS             complement(287..649)
                     /locus_tag="IVY70_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958206.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="IVY70_001064"
     CDS             complement(723..1103)
                     /locus_tag="IVY70_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP7958207.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="IVY70_001065"
     CDS             1185..2360
                     /locus_tag="IVY70_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7958208.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="IVY70_001066"
     CDS             complement(2437..3021)
                     /locus_tag="IVY70_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7958209.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="IVY70_001067"
     CDS             3222..4220
                     /locus_tag="IVY70_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7958210.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="IVY70_001068"
     CDS             4332..4841
                     /locus_tag="IVY70_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP7958211.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="IVY70_001069"
     CDS             complement(4871..5530)
                     /locus_tag="IVY70_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP7958212.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="IVY70_001070"
     CDS             complement(5532..6296)
                     /locus_tag="IVY70_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP7958213.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="IVY70_001071"
     CDS             complement(6304..6981)
                     /locus_tag="IVY70_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP7958214.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="IVY70_001072"
     CDS             complement(7018..8403)
                     /locus_tag="IVY70_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP7958215.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8372..8626)
                     /locus_tag="IVY70_001073"
     CDS             complement(8372..8626)
                     /locus_tag="IVY70_001073"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958216.1"
                     /translation="MSDYNETTTDFRVCFFYFYSTVKMNVPDFLARLIYRILYLYNLL
                     QGRKNSEIELVSMEEKAAYGMIKIKRGERVHGKNSDFTYE"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="IVY70_001074"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IVY70_001074"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="IVY70_001075"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="IVY70_001075"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP7958217.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9342..10199
                     /locus_tag="IVY70_001076"
     CDS             9342..10199
                     /locus_tag="IVY70_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP7958218.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10280..10615)
                     /locus_tag="IVY70_001077"
     CDS             complement(10280..10615)
                     /locus_tag="IVY70_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958219.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10682..12052)
                     /locus_tag="IVY70_001078"
     CDS             complement(10682..12052)
                     /locus_tag="IVY70_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP7958220.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12130..12666)
                     /locus_tag="IVY70_001079"
     CDS             complement(12130..12666)
                     /locus_tag="IVY70_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7958221.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12975..14456
                     /locus_tag="IVY70_001080"
     CDS             12975..14456
                     /locus_tag="IVY70_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7958222.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14559..15266)
                     /locus_tag="IVY70_001081"
     CDS             complement(14559..15266)
                     /locus_tag="IVY70_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP7958223.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15345..15683)
                     /locus_tag="IVY70_001082"
     CDS             complement(15345..15683)
                     /locus_tag="IVY70_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP7958224.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15794..16756)
                     /locus_tag="IVY70_001083"
     CDS             complement(15794..16756)
                     /locus_tag="IVY70_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP7958225.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKINVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16771..18102)
                     /locus_tag="IVY70_001084"
     CDS             complement(16771..18102)
                     /locus_tag="IVY70_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7958226.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18110..18790)
                     /locus_tag="IVY70_001085"
     CDS             complement(18110..18790)
                     /locus_tag="IVY70_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP7958227.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19009..19488)
                     /locus_tag="IVY70_001086"
     CDS             complement(19009..19488)
                     /locus_tag="IVY70_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP7958228.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(19615..21294)
                     /locus_tag="IVY70_001087"
     CDS             complement(19615..21294)
                     /locus_tag="IVY70_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP7958229.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            21842..23344
                     /locus_tag="IVY70_001088"
     CDS             21842..23344
                     /locus_tag="IVY70_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7958230.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23357..24019
                     /locus_tag="IVY70_001089"
     CDS             23357..24019
                     /locus_tag="IVY70_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7958231.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(24120..24323)
                     /locus_tag="IVY70_001090"
     CDS             complement(24120..24323)
                     /locus_tag="IVY70_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP7958232.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(24434..25618)
                     /gene="iadA"
                     /locus_tag="IVY70_001091"
     CDS             complement(24434..25618)
                     /gene="iadA"
                     /locus_tag="IVY70_001091"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP7958233.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(25775..27514)
                     /gene="yfcC"
                     /locus_tag="IVY70_001092"
     CDS             complement(25775..27514)
                     /gene="yfcC"
                     /locus_tag="IVY70_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP7958234.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
BASE COUNT         8216 a         5761 c         4671 g         8949 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga taactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 cttttttttc tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac
     9361 ttgctcttta cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt
     9421 cttttgcaat tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa
     9481 ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc
     9541 actatctttt taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc
     9601 acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat
     9661 ttcgctattc tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa
     9721 taaaccgtga ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg
     9781 attttgatac gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg
     9841 tccctttgtt tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc
     9901 acagccgtac tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa
     9961 aagatagtaa gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga
    10021 tcattcaaga attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc
    10081 ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc
    10141 taaaaaaagc caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa
    10201 gatagattaa aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt
    10261 gttcaaaagc tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta
    10321 catcttcggt tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt
    10381 tgatttttgc taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga
    10441 tttctagtaa tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc
    10501 gagtagtcat atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta
    10561 cactggcagc ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta
    10621 tcagctcctt tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca
    10681 ctcagtgaaa tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc
    10741 caaatccgac aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt
    10801 gaaatgtttc tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta
    10861 tattctctat cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt
    10921 tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta
    10981 actcaggaac ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat
    11041 gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac
    11101 ttttttttcg tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg
    11161 caacaaaatg tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac
    11221 cagctacgct tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct
    11281 caatcgttac tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg
    11341 caaacgtaca agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag
    11401 acaaaatctc ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta
    11461 ttgcatttgg atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc
    11521 gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg
    11581 cgttcgaaac tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga
    11641 tctcattcgt cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac
    11701 caccgggagc cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc
    11761 caacgatcat tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact
    11821 ttccattgac agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc
    11881 tatggtaagt ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt
    11941 ttttcacgct tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa
    12001 gacgatattt tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt
    12061 ttcgttagac attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca
    12121 tgttgctgcc taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc
    12181 gtttttatcc caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg
    12241 caaacttccc aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt
    12301 gtgacctttg acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga
    12361 ttttgcaatg atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac
    12421 aatgcttaaa acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat
    12481 ttcttctaat atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta
    12541 tgtagaacaa ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc
    12601 ttccacgtat accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc
    12661 catcattctt acctccgtct cttttcatct acccataaat aaataacata aataattcta
    12721 aaaatattat tgataattat caaaacatat ttagttatat ccattaaaaa tcaatttttc
    12781 cgccttaaaa acaagaatat cttgacaaaa taccctagga aataaacaat agaataaaca
    12841 aggtaacaaa aaaattaaat aacgttatat aaaaaagaag gcgacactct gtgagaataa
    12901 cacagactga aatgactttc cactcacagt ttttgtctaa ctatatacaa aaacagaata
    12961 aaaaggaggt atttatgcta attgaagcaa gaaaaaaaga gttacttgtt ctacaaatgg
    13021 tcattcaaaa aaataattgt tctttacaag aattatccaa tgacttacat attccaaaac
    13081 ggacaatcaa agaactgatc cgaaaaataa atgtatcact caagcaattt ttattgattg
    13141 atgaatttat ttattctaat cataaaggag agatccaaac caaagaaacc gatcaaacta
    13201 aaaagttagc tgttttttct aagttaaaat tatgctacct aaaagaatcc aatcgtttta
    13261 actatttgat gttactaatc aattatccac aaactgctgt accaaaaaaa tatttacttg
    13321 aacaacttta tatatcgccc tcctatctcg aaaaactgac aaaacagctc aatcattcct
    13381 taaaaaaatt ccatttacgg atcctatctg taaaaaattg ctgtactctt gaaggaaatg
    13441 agttatctat ccgtctctat ctttactttt ttctttcaga tacatttgga ggtaacgatt
    13501 ggccattttc ctcacttgaa ttaaatgcac taaaaatgaa cagtacagat gagcaaatag
    13561 aaaaaatttc aaaacaagaa aaaaatatct acttgctgtt aactttattc atattacgaa
    13621 acaaacaaca ggcatatttt ccaaaaactt cttctgaaat cactgaattg atggacttat
    13681 tgcagcatac acatgattat tcagaatact ttacttcttt ttcagtgagt cgcccttgtg
    13741 aagagacaga aacagagaga gcctacttta attatttttt ctattgtttt tttccaaatc
    13801 aaatctcttt tcgacaaaaa caggagattg gttttttatt ttccacaagc aaaaacctta
    13861 tttgtcgaca agtcggtcaa ttgatgactt acttaactca acagtttcct ctcaaccatt
    13921 tagaaaataa aataccaatt tatttttatt ttcttgtgtt ggcagttgtt tttttcgaac
    13981 tgttgggtga atccagcagt agtttttcta cactttgctt tccagaagcc acgtccaatc
    14041 attctagttc ttttatacag gaaaagaaat taagagaaaa aatcgataca attttttcaa
    14101 aaaatgggga aagaaaccaa atcagtcaat accttagtca attgatccaa cacttactac
    14161 aaacagaaac gaaagagcag ttactcatcc atttgcagat aaccaaacag tttacaggaa
    14221 attatcatct tcaaagccat ttgcagcagt tatttcgcaa agatgctgtt aaaattaccg
    14281 catattcaga acatgctgat ttgatcatta cagatcagca tgagaaaatt tcagatgaaa
    14341 cggagacttt ctatctggat acaacgcaca atacagtgtt atttggcaag ttaataaaag
    14401 cggttcatga actgtatatg aaaaaaggtt atgagaacaa aacaaagagg atgtgacaaa
    14461 attcttgtca catcctctta ttccaaataa atggtattca atcagctaac tctcggtatt
    14521 agctcagatc aaagcgctga tttcacaact tctttatttt aatctcttcg gttgccgccg
    14581 aacaataaga tcaagcgcaa cagctgcagg aatgtcgaaa gcgctgctgc aacataagtc
    14641 agtgctgctg caaataatac gtgtcttgcc atcggtactt cttcttccgt cagtagtccg
    14701 ccttcactca aaatttggat tgctcgccga gacgcgttaa attcgactgg taatgtcacc
    14761 aattggaaaa gtaatgctaa agaaaaagcg aaaatcccga tattgattaa cgtagagttc
    14821 cagctaagca atacacctac cataatcaag gggaacgaaa tcatcgaacc aaagttagct
    14881 gctggaacaa gtgctgcacg aatcttcaat gggacataac ccgaagcatc ttgtaccgca
    14941 tgtccgcatt catgagctgc tacacctatc gcagcaactg aagtagactg tgcagttgct
    15001 tgagacaaac tcaacatctt attacctgaa ttgtagttat ctgtgagatc tccggcaatt
    15061 tgttgaacac cgacattatt tatctgttga ctttgtaata tatattgtgc tgcttgtgta
    15121 ccagtcacat tgtttttgct tctcacttga tcgtattttc ggaaagtact attgacgtac
    15181 gctgacgcaa ttccggaaat aactaatcca agaatgacca aaagaaatgt cggatctaaa
    15241 aaaccataca ttccataacc atacattttc gtttcactcc ttttcgttta ctttatttta
    15301 tcattagtag taggaaaaga atgtgaaaag accttgaata ttctttaatt ctttttcaca
    15361 aattccgatt tcagtttcat cggtccaaat ccttctacct tgcaatcgat attatgatcg
    15421 ccttctacta aacggatgtt cttgactttt gttccttgtt taatggcacc tgacgcacct
    15481 tttactttca agtctttaat cactgtcacg ctatcgccct ctaacagacg gtttccattt
    15541 gcatctaata ccactaatcc gtcttctatg acagtttctt gttcttcttt gttccattcg
    15601 ttcccgcatt ctgggcaaat caacaagcct cgatcttcat atgtgtactc tgagccacat
    15661 tctgggcaat ttggtaatgt catcttcttt ctcctttaaa atctttagtg agcactattg
    15721 taacggaaaa agaagatctt gaaaagatac atttgtgatt tttactacaa aaggcacgtt
    15781 ttcgaacgaa gtatcattct gtttttctgc tcttatacga ttggcatata tcttggatct
    15841 ctttatagat ttcctctact tctgctaaat cttttgcatt cgcaccactt gctaatggat
    15901 ggcctcctcc atgatggcgt ttagccaatt cattgatgat cggcccctta gaacgtaaac
    15961 gtacgcggta ataccctgaa ggctgctgaa caaaaatccc ccaagccagc acttcgtcaa
    16021 tcactccagg aagtgaaacg atcgaagctg tctctgaatc atcgattcca tatttatcaa
    16081 gaatttcttg cggaaggatt attcttcctg caccattttc atccacctgg atattttgat
    16141 aaagataacc ggaaagttta gcgactttca tcggcatttg ttcaatctct ctgttcaaag
    16201 cacttgcatc aaatggataa tttctcaata tcgaagcaat ctctaatgta cgggacgtcg
    16261 tggagggata caaaaagcgc cctgtatctc cgacgattcc tgcataaagt aaccgtgcag
    16321 ccttaacgtt tatctttaaa gattcaggaa acatcaaagc aaattctgca atcatctcac
    16381 tacagctact tgcttttgta tttacccaca caagatctcc ataagggtca tcattgggat
    16441 gatggtcgat tttgatcagt ttttctgcca actgatatct ttgatcactg attctgggtg
    16501 aattagctgt atcagtcacg atgactagcg ctccttggta gaagacatct tcaacttctt
    16561 gcatttctgc taaaaagcga agcccttcaa tcgtttcacc tacttgacgt atttcttttt
    16621 ctgggaaact ttcccgtaat agttcggcta atcctacttg tgatcctaat gcatcaggat
    16681 cagggcgcaa atgcctgtgg ataatgattt tatcgtattt ttttattgta tctactattt
    16741 cttgctgtac tgtcatttca ctcctgcttt ctaagaacgt tccattactt gacaaacaac
    16801 gattgctttg gaaacgatgg tgttttcaat aaacacttcg atatccagtt tagcggaacg
    16861 tctgccgatt tccaatacac gtggacgaat gtctaattcg ctttccaatt ggatcagccg
    16921 caaataatgt aggttcactt gttcaatcaa gatatttcgt ttttggttca tcaccattgt
    16981 tttttgtgcg acatcagaaa tgatttcgct aagtacgcca aaagaaatgg ttccaacgcc
    17041 attgaccatt tgtggtgcta ctacgaattt gaacttaggg ttttccaaac gatttccttc
    17101 acggtcagta tcaatcgtat ggatatttcc agatatctga tccgaaatcg tatctgcaat
    17161 ctgcggttgt cgctggacaa gctgcatcgc tttcattaca tcttgccggg taatcaagcc
    17221 ttccaatgtc aagtcatcgg aaacgaccgg catgacttct agcccatccc agatcatttg
    17281 atggctaaca gaggcaatac tcattgtttt ctttacactg attggttctt tcgtcatcac
    17341 ccgatcgatg atctggtttg gcgatttccc taaaacatct tttgccgtaa caatccccat
    17401 caaacgatga tggcggttga cgactggaaa acgcgaatgc tgcgtttctt ctgacaaacg
    17461 ctgataatca gaaatcgtat ctgtctgctg caaataatga gtcttttcag ctggcatata
    17521 gatatcactg acaagcatga tatctttttt gatcaattgg tcgcttaacg ctcggttgat
    17581 catggttgcg actgtaaatg tatcataaga ggtgcgtaag atcggcattt caagatcatc
    17641 agctagcttt gcaatctcag cagtcgtatt gaatcctcct gtgatcaata ctgcggcgcc
    17701 atcttccaat gctaattttt ggacttccgc ccggttgccg acgatcatta gtgatcctgg
    17761 cgtaatatag cgttccatgg catgtttagt catcgcgccg atgacgaatt tattcaagac
    17821 cttgtctaat cctgccgccc cgcctaaaac gtccccttca atgatcctga cgacctcgcc
    17881 aaatgtcaaa cgttcaatat gtttttttaa tttgcgttcg atacggatgg tccctactcg
    17941 ttgaattgtt gaaactagtc cgacattttc tgcatctttg attgcacggt acgctgttcc
    18001 ttcactgaca ttcaattctt ttgcgatgct tcttacagaa attcgattgc caacaggtaa
    18061 actttcaata tattctaaaa tcaaatcatg ttttgtcgcc attttctgcc tacacttcca
    18121 gcgtttccga gacgctcatt atttttcctt cactatcttc cagtaactct acaaaagatt
    18181 cgggatcttg ttcaatgaca gggaaagtat tgtaatggat tggaactact ttcttggcac
    18241 gaattgtttg cgcagccttt gctgcatctt ctggccccat cgtatagtta tcgccaatcg
    18301 gcaagaaagc gacatcaata tcgaattctt ctgcaagcaa tcctagatca ctgaaataag
    18361 cagtatcccc tgcatgataa atcgtttttc cttctgcttg aataataatt ccgctaggct
    18421 cgcccatata ctgaacgata ccgtctactt catagcttga actatgctgt gcatgtacga
    18481 attttaccac gccgaatgga aacgcatgtt ttccaccgat attcataccg aatgaatcta
    18541 ctcctctgct gttcgcataa tcagcaatct caacaatact gataattgtg gcttgatttt
    18601 cttttgcaat aggaatcata tctcctatgt ggtcactatg accatgagta atcagcaacc
    18661 aatctgtctt tacttcttct gcagttaagt ctgtcaatgg atttcctgta ataaacgggt
    18721 caatcatcac gcgttggcca ttttccattt caattgttaa acaggaatgt ccatgatacg
    18781 taattttcat attttacacc tcgcgttttc tctgtatctt ttttattaaa aacgatttca
    18841 tcacttgcac atcaatgaaa aaatatagct gctaaatata ttttcattgg attgatagta
    18901 cagtatgtac aaagcaattg taacagataa tacaggatgc accaagtacc aagcagatat
    18961 aatctcaagt ttgaggtctc tgctctgctt agcttctctt ctcgtccctt aactaattaa
    19021 atcagcgtaa ataggatgga cgatcttttc taaatcggct acttttatcc gaaggcttct
    19081 cccgatttca ccgcttgaca caattacttc ttctttttct tttaaagaac tgtctgctaa
    19141 aaaacaataa tcaggatgtg cgagttttat tccaataggg gtgtttgctc cgtggggata
    19201 tcctgtatgc attaatacaa aatccattcc aggaaaacca actttacgat ctttcgtcac
    19261 ttgtctcgtt tttttgtaat ctaagtgatc gttcaaagga atcaatgcga tgatcacccc
    19321 agttttgttt cctttcacta cgagagtttt acatatcaaa gctggatcaa tccctctttt
    19381 ttgtagttct tctgtgaaag attcttctac cacatgacta aaatcaagtg gttgatagct
    19441 gatcttttgt tcttctaaat aacgttcgac ttttgtttga ctcatcattg attcactccc
    19501 tttattctat cgtttattat aaagcgaggg atgctgtatt caaaaattat ttgtttttcg
    19561 caaaaaaaga gcaagacgag cactctatag agaggttcat cttactcttt ttgtttattt
    19621 tttatttcga cgttgagtag cttccataat gactggcaag atcactgggc ggcgttttgt
    19681 ttgttcaaat aagtaacggc ctaattgatc tcggatgtct tgctttaatt tgctccactc
    19741 aaaatcattg ctttctaaat gtttctcaac gattgtttca atcatattgc tgctttcttt
    19801 catcagatca cggctagctt ttacgtaaac gaatccccgt gaagtaattt gaggttttga
    19861 aatgattttc ttttctcgcc gactgatagt gacaaccgct acgaaaatcc cgtcttctga
    19921 caagattttt cgatcacgta aaacaatgtt tccgatatca cctatgccta atccatcaat
    19981 cattacattt tctgcacttg tagttcctgc aacactcata cgcttgttct tatattccag
    20041 cacgtctccg cgtccagtaa tatagatatt tttgtatggc atccctactg catgagccaa
    20101 atctgcatga gcagctaatt cacgatactc tccttgaact ggcacaaaat atgttggttt
    20161 catcaaattg atcattaatt gcaagtcatt aggattggca tggccagaaa cgcgcatatt
    20221 ctcagagatc aacttcactg ttccaccagc tcgataaatg atatcttccg ttttcgccac
    20281 gatggtttcc atcgcgatgc ttggcgtagt agtgatataa accaaatctc cttcttcaat
    20341 cttgacatta cggtgtgtac cgtttgccat tttttgcaga gacttgatgg gttcacccat
    20401 tcggccagtt tctaaaatga tcagttcttc tggatcatag tttttcatgt ctttcaacgt
    20461 aacaagcaga tcttcacttg gtaattgaag tttttctaaa cgcatcgctg tgcgaataat
    20521 tcgttcgaaa tcttgaccag tcaagacaac ttttcgttgt gctttttctg ctgcatccaa
    20581 tacttgttgc actcgttgca aattacttgc tacacaagcg acgatgatac gcccttccca
    20641 atatttgatc gtatcaaata cttcatctgc tatttgggct tcagaagcta caggctgagg
    20701 attttctgca tttgccgatt cacttagtaa ggctaagact ccttctttgc cgatttccgc
    20761 caatcgtgca taatctgttt gatacattgg gatagccgtt tggtcaaatt taaaatctcc
    20821 cgtataaacg atactacctt cttttgtttt cagattgatc cccactgaat ctggtatcgt
    20881 atgagtggta cggaaaaagc tcactactgt ttcaccaaag tctatttctg tatgctcatc
    20941 taccacatgg aaatccttaa atcctttggc tgcttcatta cgactgacat tcaattttgc
    21001 taactcgatc gtcaactctg tcccgaatac tggtacagaa atttttgaca acaagtaagg
    21061 aagtgccccg atcgcatctg catgtccatg tgttaaaaag acaccagcta cacgatcaat
    21121 gttttcttct aaatacgtaa aatcagggat tacgacgtcg atccccaaca actcattttc
    21181 aggatatttt agcccacaat ctaaaataaa aatatcctcg cccacttctg cgacatacat
    21241 gttttttcca ttttcacgaa cgccgcctaa tgggataatt ttaatggtac tcacaaactt
    21301 cacctcttct tatgacatcc aagtttaaag atgtcgattt tcttctttat ctaaatatat
    21361 gagccattcc aatgaagcat atgcaaaatc actatttctc aacttatgga gaatcagcag
    21421 gaaaattacc agcacatccg cgttctgaca gttggatggg cattttattt tggatatact
    21481 tcattaacaa aatggatctt ttgcgaaacg ctatagccag aatacttata taagtattca
    21541 gctgttcccc attattatac aacaaataaa ctaaatcgct agtatcagca attgttagaa
    21601 caactatgca cgtaaaaatc cctctgtagc atctaggatt tcttttgcta ttcctctttc
    21661 atttcttctt cgtcggtttg aaacatactt ttttcatttt accacatttt ttgcaataac
    21721 tatagtgact ttttatatat acagaacctc atgttgatgg tgtgcaaaca gcataagatt
    21781 taagctagct ttaagatagg ttgtctatta tcaaatgcat ctactaggag ggatgcaaaa
    21841 tatgaatttt atgaaacgtg catggaaaag tacaaaagta aaatggggac gttcattgtt
    21901 attgtttgcc gtgttcacag ccattcttat ttttgtgcta gctggcctca cgattcgaag
    21961 cgctgcagaa gaagcagcga atcaagcaca aaaagaagtt ggagcaacag tgactttaag
    22021 tgccaatcga gaagcttcat ttaaaaaaca ggaacaaact tcttcaagcg gttctaccac
    22081 tgaagaacgt ccagatccag gcagtttttc tcttacacca gtttccttaa cggacgcgga
    22141 aaaaatagct gaattagata atgtaaagag ttattccttt gaaagttccg cctcagctat
    22201 ggcaaaagat ggaataactg ccatcactag ttctgattca agtgaagaca gttcagaaac
    22261 tactgatgca ggcggtgaac ctgctggagg aatgggcggg aatccgcaaa tgactcaagc
    22321 agactttcaa atcagtggtg tgtcagcaac tgcacaaaat agtaattttt ctgatggaac
    22381 agcaaaaatc acggatggtg aaggaataga tgacagcgac aaaggcacaa acaatgtagt
    22441 cattgattct actctagcag aaacaaatga cctggcagtc ggagatactt tcacaatcac
    22501 gagtactgaa gatgaagata ccacttatga aatgacgatc aaaggaattt atgaatccag
    22561 cgaaacaagc ggcagtatgg gtatgaaatt caacttcatg aatccgtcga atacacttta
    22621 cacttcttac acttttgcaa atgaactaaa tgggacatct gacgataaca cgatagattc
    22681 tgctatttac actctatctg atccagataa aatggatgag tttctcaaag aagctgagaa
    22741 gctgatcgac acagatacat ttagcttaca atcaaatgat tccatgtatc agtcaatgtt
    22801 ggaaccttta aacaacgtgg caagtttttc taaaaatgtt gttttactag tggctgtagc
    22861 cggtattatc atacttactc tgatcattat gattaccatt cgggagcgtc ggcatgagtt
    22921 aggcgtcttg ctatctttag gagaatccag aagtaaaatc attctccaat tgtttaccga
    22981 agtggctatt tgtatgattc ttgcattagg agttgcttcg cttagcggaa acgtcgtcgc
    23041 gaatgccgtg ggacagcagc tacttgacca acagacagaa accacgacac aaaaccagca
    23101 agctcctggc ggtggacaaa tgccaggtaa cagacaagaa caaggcggtc aacgcgatgg
    23161 tcaacaagga accacgaata atccatttgc tgtttctgat caagtcagcg aattggaaat
    23221 caatgtcaaa cctgcacaat tgggactatt agccggagta gcatttggaa tcagtctgtt
    23281 ttctgtcttt ttagcttcta tagggatatt aagattaaac ccaagaaaaa tcctattaaa
    23341 ctaaaggagg aatttgatgt tacggacaga acaattggga ttttggtatc aagatccgca
    23401 agatgcgctt tataaagatg tagaactgaa tttcgaagct ggaaaaatgt acgctatttt
    23461 aggagcaagt ggttctggaa aaacgacttt tctttcgctt atttctggac tagatgcacc
    23521 aaaagaagga aaggtatact atcaagatcg ttcactgaac aaaatcgggt taagaagtta
    23581 tcgtaaaaat gatgtctcga ttattttcca agcttacaac ttgcttcctt atttatcggc
    23641 tctagacaac gtcatagcag caatggaaat cactaagacc aagcaagaga attatcgaca
    23701 agttgcttta gatcgactgg ctcgtgtagg aattgatgaa atattagcaa aaaaacaggt
    23761 gaccaaactt tcgggaggtc agcagcagcg agtcgctatc gttcgtgcta tctgctgtga
    23821 acatcaacta atcgtagcgg atgagcctac tggaaatcta gatgaaacaa cttcacaaga
    23881 tatcgtcaaa cttttccaag aaatcgccca tgaacaaaat cgctgtatta tcatcgtgac
    23941 tcatgagatt gaagtagcaa aaagctgtga tgaagtcttt gaactaaaaa ataaaaaatt
    24001 tcaaaaaatc gaagtttaaa aaacaggata atgatcaaat aaaaaatccg aaccatatct
    24061 gtctcgcaga tatggttcgg attttttatt tgcttgattc aattagtaat aagtggtttt
    24121 tatttttttt cgtcttttcg agaaaacttg tctttgactt cgtcgcctaa atttcgagct
    24181 ttttctttga tgtcagcacc tagtgattgt gctttccctt ctgtttcttt gctcttgtcg
    24241 tctgttgctt tgccgtatgc ttcttttcct tcaccgacta ctttatcttt catatcttta
    24301 gaacgtcctt ttaaatctgc cattgtgctt cctcctctta gtttgtttat tgatctacct
    24361 tcagggtaac atcaacaaca gatacagaca aacagaacgc tcgttttcta aaaaagatag
    24421 ttagagctgt tgattaaaaa cttttttcga aagtccccca tactttgact tctttttctt
    24481 ggaccattat ttctccttta gcgataactg tatcaatatc aagcgtttct ttatccaaga
    24541 tacacagatc tgcatcagca ccaatttcga tttttccttt tttatccaat ttcaatatac
    24601 gtgctggatt actggttata gctcgtaagg cgatttccaa agggatactt tctttttgca
    24661 cagcttcttt gatccctact agcaatgcct tggcacttcc tactcctaac cctaggaaat
    24721 gattgttttc atcaaaataa ggcaagcttc cttgtccatc tgaggtcatc gtgaaattat
    24781 ctaaagaaac atcttcctcg atcaatcgtt tcagtgcttt gctgaagcga acttcaccat
    24841 ctgtcttctc ccaaaaatca ggatcttcgc tagcagtgaa gtcaatcgtg ccaccacgtt
    24901 tggcaaatgc cacgcaagct tcaaataatt ctggtgtgcg atttgcatga gtaggcaaaa
    24961 attgtgtgat cggaatttct gtttcttcta cgattcgtgt caatagctct agctttcttt
    25021 ttcctccgcc taagtggaca ttgatgatcc cagcttttcc ggataacatg cctcctacac
    25081 gagcatctgc tactgcacga gcgaactctt caaaacttgg ctgagaagaa cggtgatcgg
    25141 atactgcgat ttctccgatt ccgataattt tatcaattgt caaaaagtct ttgatgatag
    25201 aatctgttac tgtttttacc ggcaatcggt acgaaccttc gtatacataa gtggagattc
    25261 cttctgcttc cagacctttc gctttgctga tcaacgcagt catatctcta ccttctccgt
    25321 ctgttcccaa acaaccgaca actgtcgtta caccagctgt agtcaattgg ctcaatgtga
    25381 cttctggcgt tcgattttga taaccgcctt ctcctcctcc acctaaaata tggaaatgac
    25441 agtcaatgaa acccggggtt agtacttttc cctcacctga aagttcttct acttccactc
    25501 cttcgtagcc gccttctagc tgttcttcta cagccaaaat cttcccaccg gcaatcagta
    25561 catccatttt tcctaagtaa tctggtgcaa acacttcaat ttgtcgaatg attttcatac
    25621 atttttcctc tttctttaag tataactgat gataggatct taaaaaataa tccaaacgat
    25681 aggatcttta ctcctttgat tttaccgtaa aatgacggtt cttggagtcc ttatctactg
    25741 tttggattac ttttttattt acaggtctcc agtactactg cagacctatt gcaatggcaa
    25801 tcatgatgaa aatagttcct aaaacaaaca ggaatccttg catcttgatt tggaatcttg
    25861 cccattttgc ccattcgatc cgtgcaacac ctagcacacc aatcaagctg gctgatacgg
    25921 gtgtaaaagc gtccataaaa cctgaaccaa gctgataagc aagaactgct acttgtctgg
    25981 atacacctac taaatctgcc agtggagcca tgattggcat agtcaaagct gcttgtcctg
    26041 aatttgatgt aacaaccaag ttgaacaaac tttggaagat gtacatcgcc caagcaccaa
    26101 tgacagctgg aacaccggtc aacgccgtcc cgatcccatg caaaatcgta ttcaaagctg
    26161 aaggaacaga tgcatcagat cctcccaaga caagaagaat ccctttggcc atacctacta
    26221 cgattgctgt accagctaaa tcagctgcac cagattggaa agaagaagcg atgtcattga
    26281 ctttcattcc atctaatttg aaaagaacag caatgattcc agcaactaat cccatcacaa
    26341 agaattgaga agcgatttct ggaatgtaat agcctttttg agtcacgccc caaacgatcc
    26401 agacaaggac aactaacatc tctaataaga tcaatttatg cccaagcatg aattttttat
    26461 tttcatcaga tgtctttttg atatgagaac ggaaataatc atctgatttg taagtcaacg
    26521 aactttctgg ttttttacgg attttttctg catatatcat catataacca gcggctaatg
    26581 cagtaacaac tacccacata atcaatcgga atgtcgcacc agacaacacg gggattccag
    26641 caatcccttg agccaccgct acgctaaacg ggctcatcca agaggtcgca tttccgactt
    26701 gagatgcgac ataagtcacg gttactgcta cgatcgagtc atatcctaac gcaatgacaa
    26761 atggaaccat caccattgag aatggaatta cctcttctgc catcccgaat gtagcgccac
    26821 cgaatgaaaa agcaaagaat aatagcggta aagctaaacg ctccagtccc ttagttttac
    26881 tgataaacgc atagatacct gcgtcgatcg ctccggtacg cataataata ccaaaagcac
    26941 ctccaacaac aaggatcaat gcagcgatcc ctacagcaga tccatactta tctccagata
    27001 ctagtccttc aaaaacaaaa tttaagaaac caaagccgcc aaaatcatct gttccccata
    27061 ttttggctgt tttatgcaat tttttagaag tatcatagat attttcgccg tactgatcgt
    27121 aaagcacatc atctgtcaag gagatttcat ctagtttttc ctgcgtaaga tttttttctc
    27181 cgtcagcaat gactttttcc aatccttttt ccggaacatc cagtttttca cgttctgctg
    27241 gatgatcttg cagttcttct aattgatcaa acacatacga cttatccagc tcgtaagcat
    27301 aacggaagga atcttgacgt aaaacagtac gagtacttgt ttcgccattt gcatctttgt
    27361 attcaatatc ttgcgtgctg aactttcctg ctggaacgac aaaagtcagt atccatgcaa
    27421 agatgactac accaaaaata atgacatacg tatggggtgt cgataattta gtgaagtcgc
    27481 gtttcggttt tcctgaagaa tttgttttct tcaaatttat ttcttccttt cttttttcag
    27541 taattttgat gaataaaaaa tggataatca aatgaattat acagtaaaaa tgacatt
//
LOCUS       DACCXO010000027        27017 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.028, whole genome shotgun sequence.
ACCESSION   DACCXO010000027 DACCXO010000000
VERSION     DACCXO010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.028"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..1254)
                     /locus_tag="IVY70_001093"
     CDS             complement(<1..1254)
                     /locus_tag="IVY70_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7958235.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENR"
     gene            1486..2478
                     /locus_tag="IVY70_001094"
     CDS             1486..2478
                     /locus_tag="IVY70_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7958236.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            2538..3959
                     /locus_tag="IVY70_001095"
     CDS             2538..3959
                     /locus_tag="IVY70_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP7958237.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            3952..5451
                     /locus_tag="IVY70_001096"
     CDS             3952..5451
                     /locus_tag="IVY70_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP7958238.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            5490..6884
                     /gene="uxaC"
                     /locus_tag="IVY70_001097"
     CDS             5490..6884
                     /gene="uxaC"
                     /locus_tag="IVY70_001097"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP7958239.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            7043..7726
                     /locus_tag="IVY70_001098"
     CDS             7043..7726
                     /locus_tag="IVY70_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP7958240.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            7728..8564
                     /locus_tag="IVY70_001099"
     CDS             7728..8564
                     /locus_tag="IVY70_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP7958241.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            8575..10329
                     /locus_tag="IVY70_001100"
     CDS             8575..10329
                     /locus_tag="IVY70_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7958242.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(10605..10946)
                     /locus_tag="IVY70_001101"
     CDS             complement(10605..10946)
                     /locus_tag="IVY70_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP7958243.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(11014..13185)
                     /locus_tag="IVY70_001102"
     CDS             complement(11014..13185)
                     /locus_tag="IVY70_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP7958244.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            13396..14253
                     /locus_tag="IVY70_001103"
     CDS             13396..14253
                     /locus_tag="IVY70_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP7958245.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(14324..15181)
                     /locus_tag="IVY70_001104"
     CDS             complement(14324..15181)
                     /locus_tag="IVY70_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7958246.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(15166..17868)
                     /locus_tag="IVY70_001105"
     CDS             complement(15166..17868)
                     /locus_tag="IVY70_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP7958247.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(17881..19170)
                     /locus_tag="IVY70_001106"
     CDS             complement(17881..19170)
                     /locus_tag="IVY70_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP7958248.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            19320..20366
                     /locus_tag="IVY70_001107"
     CDS             19320..20366
                     /locus_tag="IVY70_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7958249.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            20368..22521
                     /locus_tag="IVY70_001108"
     CDS             20368..22521
                     /locus_tag="IVY70_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP7958250.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(22994..24445)
                     /locus_tag="IVY70_001109"
     CDS             complement(22994..24445)
                     /locus_tag="IVY70_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7958251.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(24442..26166)
                     /locus_tag="IVY70_001110"
     CDS             complement(24442..26166)
                     /locus_tag="IVY70_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7958252.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINSHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(26159..26773)
                     /locus_tag="IVY70_001111"
     CDS             complement(26159..26773)
                     /locus_tag="IVY70_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP7958253.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
BASE COUNT         7942 a         5444 c         4945 g         8686 t
ORIGIN      
        1 tcgattttcc tttggcacaa tttccgtgat catgttgttg atcgctaaaa gtagcaaatc
       61 acgctcccca ccgtgactat cttctaacag atcgatttga accaaagctg tataatagca
      121 gtcattttga gaactataac caaattgctg caaccgctga tctagttctc gcctttctac
      181 tcgatttcta aataaactaa gtacaaatag cgtttccagc tgtggctttt gggtaactaa
      241 gtgtgcactt aacgcttctt tttctcctat gatccgactg actgattgag cgaccaaatc
      301 tagttctttc ccactgaatc cttttgattt aagattcaga cgttcctgta tttgagaaat
      361 cggacgggca aatctgtccg aaaataagta ggaaagaaga ccaatgagtc ctaccagtgt
      421 taaagacact gcaactagcc cgattctcag attgcggatc attgcgccaa ttgcactaga
      481 gtcaacttct aatctgtaaa tccaattatt ataatctgat ttcagtacta tatacgtttt
      541 atctttttct cttatcactt tcagctgatt gtaatctaga tccacgtccg ttaatacagt
      601 tggttgctct ttatttttta ctgtctgagt agtaaaaagg ttgccaactt cgttttcaat
      661 agaaagtatg tcttctccac tttcaccaac aacttgttct attgtatgtt tctctatcgt
      721 agcaattccc aatgcatatt tttctttttc aaacattggc aaagaaatca ccatttcgat
      781 tcctttgttt ttcttttgcc aaaataaatt atttttgtta tttatgtact tctgttgata
      841 cttttcaact tcctcttcgg tcagttgact caacgatcca ttgattactc cccatttacc
      901 agccaaactg actaaatcat aattattatc atccataaca atcgtttcta taaaatttag
      961 ttctttcctg atttcagaat aagtggagta atctgtatat gtcagcggat gcttgaatat
     1021 gtccgagaaa tttttggtcg aaccatacgt agaaaacgaa tattcaatcg ttctaatttt
     1081 ccattcaacg ttagttttaa cttgcctgag caaattcttt ttgtctgtta aataactcga
     1141 ttcgatcgat tcataggtat tctggtaaat gaataaacta aatcctatga caagtacggc
     1201 tcctaataaa aacatcggta aaaatatttt ataaaatact gtacttttct tcattttctt
     1261 cctctcctcc ttttaaaata aaaggctttc atttttctgt aaaattgata ctcatctaat
     1321 taaatgagca ttccccgctt tcaagaatag cataattaaa aataaaaatc caagtaattt
     1381 ttaacgtgtg caatcttttc atatcaaaga tctattgact tatcgtagtt atcgtaatat
     1441 gatgaatgcg ttaacaaaaa gtgaaaaagt aaggaaaatg atggaatggt aacgatcaaa
     1501 gatatcgcta aagcagctgg cgtttctcat acgacagttt ctcgtgcttt gaataacagc
     1561 agtttaataa aaaatgaaac aaaacaaaaa atacgacaac tagctaagga aatgaattat
     1621 tcgccaaact atagtgcaaa aggtttagtg aatcaaaaaa aatatctaat cggactattt
     1681 ttctcaagtt tgcataaagg aacttcatca aatttcctag cagattccat tgcaggcatc
     1741 cattcagtat tagacacaaa ttacagttta tctgttgaaa gtatcgatgt tgttgattta
     1801 acagaaatca acctgcaacg ctacgatgga ctgatcgtta tgagtcaaag tgatgatgat
     1861 cagcctttca tcgactatct caaagaaaaa gaatttcctt tcgttgttgt caatcgtcac
     1921 atcaatgatc cagcggttat caacgtcact gccgatgatg cggccggtgt tatgcaggca
     1981 gtagattacg gtattggatg cggacatacg aagatcgctt atattggcgg aaagaaaaat
     2041 tttcgttcga caaaagaacg ggaaaaaggg gtattgaaaa gtctaaaaaa agcgagcatc
     2101 cctgtcaatc cagcctattt tttaacagga gattacagct tagaaagcgg cctccaaaac
     2161 atggaatacc tattatcact ttctgacctg cctactctgg ttttttgcgg caacgacgac
     2221 atagcgattg gtgcattgag agctgtacat ttagctggcc tccgtgttcc ccagaacatt
     2281 tctctgatcg ggttcgatga ttctccagtc gtctcctatc tcaatccgcc gcttaccacc
     2341 gtacgtaagc ctttgaaaga aatgtgtcaa gaaggaacaa agcttttact agatttgatc
     2401 gaaggcagga cgatcgaaca atctaaaatt gagctctcca ccttgttgat ccttcgtgag
     2461 tcgatcaatt acttgtaaac tacaagtaat tttatataaa aatgttaacg tgtgcataac
     2521 acagaaagga agtttatatg aaaaaactga caaaagaatt agtgaaaaaa aacgaagcac
     2581 ctgtaaaagt tcttcagttt ggtgaaggca attttatgcg tggatttatt gattggcaga
     2641 ttcagcagct gaataaccaa caattgttta aaggtaatgt tgcaatcatt caacctttag
     2701 aacaaggtct tggaaatatg atgaaccagc aagataattt atatacggtc attttacaag
     2761 gactcagcaa tggcgaaatc gttgatactt ccgagattat caccgtagta gaacaaatga
     2821 tcaatcctta tgatgaatgg gatgcttatc tatctctagc agaaaatgac gacttggcct
     2881 atatcgtctc caatacaaca gaggcaggaa tccaattcgt ggagactgat aatttaacag
     2941 atactccccc acagagcttc ccgggcaaac tgacagcctt tttataccga cgtttcaaat
     3001 taaacaaagc tggctttacc atcattcctt gcgaactgat tgacagaaac ggcgaacgtc
     3061 taaaagaaat cattttacgc tacgcagaaa aatgggattt agaacctgct tttgttgagt
     3121 ggttagaaaa agaaaatgtt ttctgttgta gtttagttga tcgcatcgtc ccaggttatc
     3181 ctcgcgaaga tgcaggtgtt ttcaatcaac ggcataacta tgaagatcaa ttgatggtca
     3241 aagcagaacc attcatgctt tgggtgatcg aagggcctaa agaattggag aatcaactac
     3301 cattcaaaca ggctggcttg aatgtcatca tcacagatga tatgactcct tatcgtgaac
     3361 gtaaggtgca tttgcttaat ggaccgcata cagcgatggt ccctcttgct ttgttggcgg
     3421 aattggaaac tgtagaagaa gtaatgaaag atcctttatt ctccgcatat gttgatcagt
     3481 tattcaatct agaattgatt cctatgcttt ctttacctaa agatgaatta gccatttatg
     3541 ctgaccaaat caaagaacgt ttcttgaatc cttttgccca tcacaaatta gaagcgattt
     3601 cattaaatag tgtttcaaag ttcagcaccc gtctgcttcc agtgttcaag aagtatatcg
     3661 aagaacaaaa tcaggtacca ccgttaatca ctgtttcact agctgcgcta cttcttatgt
     3721 atcgaggcga tcaagtaaag ccacatgatg atgaaaagac gataagcgaa tttactgatg
     3781 cttggtctga taatggaaca gctattcctc gattattgca aaacgctgcg ctttggggag
     3841 aagatttgtc tcaaataccg aatgtgacag atacagttca agaaatagct gcaaagatcg
     3901 atcaaaaagg tgtccggtca gtattacaag aaatgaaagg aacgatggtc catgcttgaa
     3961 accctgcaac caacaatgaa attaaatcga cgagacagtg tggtcgtcgc attaacccct
     4021 attccaaaac aaacacaact gacgattgat cagcaaacga tcaccactct tgaagatatc
     4081 ccgcaaggtc ataagattgc attgggtgat ttgaaagctg gagacaacgt gatcaaatat
     4141 ggctatccaa tcggtcatgt gacgacagac gtaactgccg gtcaatggct gcacactcat
     4201 aatgtaaaaa ccaatctttc tggtgaattg gattatcgat atgaaaaaga tgtccatcca
     4261 agtcattacc tttttgagaa ccgaacattt caaggttact tgcgaaaaaa tggaaaagta
     4321 gggattcgta atgatttgtt tttagtacct accgttgggt gtgtgaatgg cattgctgaa
     4381 ctgatcgtca aacagttcaa ggaaaaacat ccagatctgg gacagtttga ccatattacg
     4441 attttaaagc atccttatgg ctgttcgcag cttggaaaag atcatcaaaa tactcgagaa
     4501 attttagcgg acgcagtaaa tcatcccaat gccggaggag ttttagtatt tggattaggt
     4561 tgtgagaaca atacagttcc tgaattcaaa aaaattttag gtgaatatga tgaagaacga
     4621 gtgaaatttc tagtggctca agatgtttac gatgagatcg aacaaggtgt ggctctatta
     4681 gaagagcttt taacagcggc ggaaaatgac cagcgcatat cagtcccgct ctctaagttg
     4741 aatgtcggtt tgaaatgcgg cggttctgat ggtctatcgg gaataactgc taatccttta
     4801 cttggtgcat tttctgacta cttgattgct caaggaggca gcacgatctt aacagaagtg
     4861 ccagaaatgt ttggcgcaga acaagtattg atggcacgag cggaaaatga agaagttttt
     4921 gactcaatcg ttgacttgat caatgacttc aaacagtatt tcttgtctta cggggaacca
     4981 gtttacgaaa acccatctcc tggtaataaa gcaggtggga tcactacatt agaagataag
     5041 tctttaggct gcacgcaaaa atcaggaagc tctcctgtcg ttgatgtgct acaatatggc
     5101 gaaaaaatca gaaaaccagg gcttagttta ttgcaagctc ccgggaatga tcttgtagct
     5161 gcctctgctc ttgcttcatc cgattgccag ttggtcttgt ttaccacagg aagagggact
     5221 ccgtttggct catatgtccc aacggtcaag gtatctacga atacaactct ttttgaacgc
     5281 aaaggtcatt ggatggattt taatgcaggc gtcttattag aacaaccaat ggaagtaatc
     5341 ttagaatcgt ttgttcaaaa aatcatcgct gtagctagtg gagaagaaac gaaaaatgaa
     5401 caaaatgacg ttcgagaaat tgccattttt aaaaacggag taacactttg attttcataa
     5461 aacagtataa aaagaaagaa ggaagattta tgtttttatc tgatgatttt ttattaaaag
     5521 acgattgggc gaaaaagctt taccattcct acgcaaaaaa catgcccatt attgattatc
     5581 actgtcactt agatccaaaa gaaatttacg aaaacaacaa tttcagcaat ctgacagaag
     5641 cttggttagg aggcgatcat tacaaatggc gcttgatgcg agcttgcggc gtgcctgaag
     5701 aaaaaatcac aggaaatgct tctgattttg aaaaattttt agcttggtgt caaaccgtac
     5761 caaaaattat cggaaatccg ctttacagtt ggtcacatct tgaattgaag cgtttcttcg
     5821 gtattgactt gttgataaat gaagaaaatg cagagaaaat ttggacacaa gcaaatgaaa
     5881 ttttggcagc tcctgatttt cgccgtcggg aaatcgcaaa aaaatcgaat gtagaagtga
     5941 tttgtacaac agatgatccc gcagacgact tgcactatca tcagttgctt gctcatgaag
     6001 aaccagattt gaaggttcta ccttcattcc gtccagataa agcactcaat attgaaaaag
     6061 aaggatttaa tgcatgggtc acactactgg aagaagcagc tgaccaaacg atcaccacct
     6121 atcaagaact gatagaagtc ttagggcagc ggatagaata cttccatcaa catggatgtc
     6181 gtgtttccga ccacgcttta gacatcttac gctataaaga agcggatgaa acagtattag
     6241 aagagatttt tcaaaaagca cgagctaaca aactattatc ttcagatgaa atcgatgctt
     6301 atcgtacaga aacactgata catttgaccc acttctatca ttctcataat tggacgatgc
     6361 agctgcacat ccatgcttat cgtaactgta atacacaaat gttggaaaaa ttagggcccg
     6421 ataccggcta tgatggaatg aatgatcttt ctttgactat tcctctgcaa aaattattga
     6481 accgtgcaga agaaacggat cagttgccaa agacgatcct ttattcattg aatccgaatg
     6541 actatcctgc gattttagct ttgatgggct gtttccaaaa agaacagaat ggaaaactgc
     6601 agttaggttc tggatggtgg tacaacgata ctcgagctgg tatgcgcgat caattgacac
     6661 aatttgctga tggtagtgct ttggggaatt ttgtcggcat gctgacagat tcaagaagtt
     6721 tcctgtccta cactagacat gaatatttcc gtcgtgttct ttgtgaattt ttaggcgaaa
     6781 tggtcatgcg aggagaagca cctgaagatg aacaactttt aggaagtcta gttcaagcga
     6841 ttagttatac aaatgctaaa aattactttg gcttttttgc ctaattgatt aatagtaaca
     6901 gtaacgaatc aaaaacagga tagtgaaata acggaatccg tttatccacg ttttagagaa
     6961 gaagacggac caaatttgct ccgtcttctt ctcttgctgt tctgcttcat tgcctataaa
     7021 cctttatgaa agaagagata acatgcctac tatccatatt gccggtgatt ctactgcggc
     7081 agaaaaagat gaaaacaagc gtcccgagac aggttgggga gaaaaaatct ctgcttattt
     7141 ttcacccgag atccagatca ataaccaagc aaagaatgga cgcagcacca aatcatttat
     7201 ccaggaagga aggttagccc gtctagcttc acagtttcag ccgaatgatt tcttatttgt
     7261 acaatttgga cataacgacc agaaaatcca agaagaaaga ggtacgcttc ccttcggtag
     7321 ctatttaaaa aatctgcaga tattttttga atgcgcaaaa aaagcgaacg tccaaatggt
     7381 cttactgaca tctgtgacgc gacgagatta cttagaaaac ggaacattga atccagatat
     7441 acttggagac tatccaaaag cgatgcgtgc atttgctgag aaacaccata ttcctctttt
     7501 agatgtgttc tcaagatcac aagaattgtt ccagaccttc tcaaaagaag agacaaaaaa
     7561 attctacctg catcttatac ctgatacttg taaaaattac ccagaaggac taaaagacaa
     7621 tacccacttt agtccagaag gcgctgataa agttgcccga ataattgttg aattgataaa
     7681 agaatcccgt ctgcctcttg ctaattattt acaaaaagga gtttgagatg tttccattaa
     7741 atttaggcat ccgtgcccac gatttagatg ttcgaaatcg tgaagaaatc gttcaaaaag
     7801 tatcacagct tcacttgtcc catatccaat ttgcaccaca aaaagcattt cctgaacaac
     7861 ttaaattaac tgacatgaca cttgggagtg ctgcctattt tggtgaatac ttccaaaaaa
     7921 acaatatcga actttccatt ttaggatgct atatcaacct atccagcaaa aatcaagaag
     7981 ttcgtgcaca agcattggca actttcaaac agcaaattgc tctgtgctct gcctatcaag
     8041 ccaagatgat tgctacggaa accggtagtg tctctgtcgg ttacacaaaa gaaaatttta
     8101 ctgaagaagc ttatcagatc gccagaaatt ctatcattga attagtcgct catgcagaaa
     8161 attttggcgt aactgttgct gttgaagcag ggatcaatca tccaatctat aactatcagt
     8221 tagctaaacg tttaatcgat gaagtagctt caccaaattt aaaaatcatt ttagattgtg
     8281 cgaatttgat gcataaagaa aatcatgctg atcaggaaaa agtgatccga ggtgccttgg
     8341 acaatttaca tggttatatt acagcgcttc atctcaaaga ctatatcatg gaaaatggaa
     8401 agatccgcat ggtacctgtt ggcaagggct gtttagattt ccgcccggta ctgcattata
     8461 tcaaatatga gcgtcctcta atgtacgcca ctcttgaagc tactcctgag atttatgttc
     8521 ctgaagcaat agaacacttg cagtttattt accaagattg ttaaggagaa gattatggag
     8581 gaacgaatac agaaaagtta tcaaaaatat catgcaaatg cttttcagac attgctgcat
     8641 ctttatcagg gaaattattg gcgttttgcc atgtcaggac tcttttttat tatcaaacat
     8701 cttccatctt ggttaatgcc gatcgctatt gccaatgtaa tcaatgcagc ttcatcgaaa
     8761 gattcttctc agttgcaggc catttatttc aacgcttttt tgatggcttg tctgattggt
     8821 caaaatatat tgacgaatta ttttcatgta aaataccata gcacttcgat ccgtcaagta
     8881 gaagctggac taagagcgac ccttatcaag aaaatccaag aactatctat tccttatcaa
     8941 aaagaattgc aatctggacg aatccagtca aagatcatcc gtgatgtaga agctatccaa
     9001 acgttgtcct ctcaagtatt tgtcagcgga ttaaatatcg ctgtcaattt attcgtagct
     9061 ttaggtatca cgctttataa aagtcctgtt gttttcatgt ttttcttatt gactgttcct
     9121 ctcgccgcat tcgttactct ctttttccgt aaaaggatca gacagtccaa ctcagacttc
     9181 aggcaagaaa tggaagagac ttctgcaaaa gtgatcgaag tgatcgaatt agcaccgatt
     9241 gctcgagctc atgggttaga aaaacacgaa acaactcgct taaataagca ttttactcaa
     9301 atctatgaaa aaggattcca cttagatatc ctgcaatctg ttttcggatc agtcagctgg
     9361 gctagttttc agatatttca gctgatttgt ttgattttca ctgggatatt agctttacaa
     9421 gggcgcatcc aacccggcga tgtcgtgatg tatcagacgt atttcacgac gatcgtcaat
     9481 tccgtttctg gaatgatcac gctgattcct acgatttcaa aaggcatgga atctatcact
     9541 tctattggcg aactgttgat tgctggcgat gtagaaactt atcacggtaa gaaaaaaatc
     9601 caacatttgg atggaaattt tcgttttgag gatgtttctt ttctttatcc ggactcactc
     9661 aaaccgatta tcgatcattt ttctctagag gtgaaagctg gagaaacaat tgcttttgtt
     9721 gggccatctg gctctggaaa atctacactc cttaacttat tgattggatt tatccagccg
     9781 acttcaggaa aaatttttct agatgatcag cctttggatg aactggatat gcgaagtgtg
     9841 cgaaattatt tagccgttgt tcctcaaacc actttgttgt tctccgcttc gatcaaagag
     9901 aacatcactt atggcttgaa aaacgtatca aaagaacgtt tagatgaagt catcgaagct
     9961 gcgcaacttt cttcactgat tgaccagctt cctgatggtc tggatacctt agtaggcgaa
    10021 catggcaaca agctgtctgg tggccaaaag caacgtatct ccattgcacg agcgttgata
    10081 cgtcagccaa aaatcatttt gctagatgaa gcaacctcag ctttagataa ccagtcagag
    10141 aaaaaaatcc agcaagccct gaattacctg acagaaacac ccactacttt tattgtggct
    10201 catcggcttt caacgatcaa agaggcagat aaaatcgttg tgatcgatga aggaaaaatc
    10261 gttgaaatag gtacttacga aaaactgtta gctcaaaaag gctatttcta ccgcttgaaa
    10321 aatgaatgac aaaaaatagg agctatgaca agacttttgt cgtagctcct atttctgaat
    10381 aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atagtcatca aacatgagaa
    10441 gctgtttttt gaaattgttc cttgtaactc agagttaaac ggtaggctca cgacctcttg
    10501 gtaaatggtg ttcaagagac agcctatcgg caataagaag caattttgtc atcgctcaag
    10561 gccgaacgtc tctttcacac cctctgttgt ttcataaaat agttttattc gctgtaaagg
    10621 tcgcgaactg gtgtcatgat gatacggttc aagtcattga tgattgtact gaagccttgc
    10681 tctttttgca tcaagtcatt gattacttct tctgattgga ttttcacagc catttcttgt
    10741 gctttcattg catcttcgtc gctgaactct tgtccactca tttgtttttc ttgtaattcg
    10801 atttgcatcg cttggaagtc tttgaacagt ttgtgtgctt cttcgtttgc ttgtactttc
    10861 tcgtacgctt ctttcaacgc tttgaattct ggtaattcac ggatctcacg ttcgatttga
    10921 ttcgcgctat cgtagatgtt tgacatgtta tgccctcttt tcttttagta gttactctct
    10981 attgtaacat ggggttattc gttttcaaag gggctactct ccttgatatt tccgccagag
    11041 gtctttagcc gcttcaccaa ttccctgaac gccttctttc accatatcgc cgaagttttt
    11101 cagcccttct tttgcttgtt caccatattg attcaagtta tcttgccaat ttccagactc
    11161 tccattagaa tttcctgtgt ttccaacttg atctgcagct accacttgtc ctcctgtggc
    11221 ataggcatct gctacactaa atcctgtttt tgaagtatac ggaaggatgc cttctgccgc
    11281 tgatttgaag actttcccga caacatttcc acttgtccct tccaaatagt gcagcttgct
    11341 ggattcttcg taacccatcc atgtcgaaat cactacgtct ggcgtgtaac cgatcatcca
    11401 ttggtcattt gcttttgtgg catcgaaatt agtttctgtg gttcctgttt tccctgctat
    11461 cgtataacct gcaggattcg cagcagccgc tgttccattt gaaaatgtac caagaagcat
    11521 gctggtcatt tcatcggctg tttcctttga tatgacctgt tttggttttt tgtttgtgtt
    11581 atcgacgatc accgctccag tcgaatcaac gattttcgtg ataagatgcg gcgtatacat
    11641 ctgaccatca ttggcaaatg cactgtaagc acccgccatg atcaatggag atactccttt
    11701 ttccagtcca ccaagggcta agccataata cttatcagat tttgctaaag gcaggccgaa
    11761 ttcttctgct ttatcatatc ctttttgtaa accgatctca tgcaaaagcc atacagtagg
    11821 caaattcaaa ctttgtgcaa cagcttgata catcggtact tctccttggt atgtcccgtc
    11881 aaaattatgc gcatcgtaat aactttgcgg ttcgtctttc aagatactgg aaggcttgta
    11941 tccagcttcc agtgctggtg tatatacgct taatggtttg atcgttgatc ctggtgaacg
    12001 acgcatctgt gtagcaaagc tgaatcctct aaagacatga tctcctcttc gtcctactag
    12061 tgcttgtaca ccacctgtat tcggatctaa agctactgaa gcagattgga ccatggcacc
    12121 atctgaagca tttgctggga ataatgcact gttttgataa gtagcttcta attgcttttg
    12181 ataatcctga tctaaagctg tatagatttt atagccttta ttcatgacat cttcttcatc
    12241 aagaccatac tcgttaactg cttcatcgat cactgcatca aaataataag gataacgata
    12301 accagaatct gaattatcgt atgtgtcatt aagatacgcg ctgatatctg tctggctttc
    12361 tgcttgttct tgttcttttg tgatttttcc gttttcttcc attacactta gtactgtgtt
    12421 tttccgatca ttcgcatttt ttgggtgatc gatcggatta taaataccag gacctttcag
    12481 cattcctgcc aaagtagcag cctcagacag cgtaacattc gttgcatcta ctccaaaata
    12541 ctttctggaa gcgtcttgga caccccatac cccgttgcca aaataggcat tgttaagata
    12601 catggtcaag atttcttctt tgctgtactt cttttcgatc tcgattgcca aaaataattc
    12661 cttcgctttt cgatcaaacg tttggtctaa tgtcagatac gcatttttgg caagctgctg
    12721 agtgatcgta cttcctccgc cccctgatgt tccgccagac aagaccattc ggactgctgc
    12781 acgggcaatc cctttgatat caaacccgtg atgctcgtaa aaattcctat cttctgtcga
    12841 gattactgca tcttggatat agggagaaat cgcgtttaat tctacatatg ttcctttttg
    12901 ggcgtacagt gtgccagctt cttcgttttt cttatcataa atgactgtcg attggcttaa
    12961 tcctgatttt aacgtttcga catttgcttg ttttgctaaa ataaagaggt agatacttgt
    13021 cactaagaca cagatcaaac caatcagcaa aatcaatttg ttcacatgat attttttcca
    13081 gatgcgcttt cgccatcgat ggaattgtcc gagatatggt ctgatccaac gccaaaaact
    13141 gatcaaagca gcttttatct tttctaaaat gctttgaaaa tccatttgct tcttctcctg
    13201 tctatttgat tcaatcgtct tattttacca tagtgaaaga gtgttttctt cctgcttaag
    13261 ttgtagttca atatgttttt ttacgttagt gcaagtagag taacataaga atgaacagaa
    13321 aaatgtaagt tttttcttaa atatgtttta aaaatggtac aattagtctg cgattgcact
    13381 gaaaggagta cacttgtgaa aatcacgatc actttaccta tgaaccatcc tgaaacaaca
    13441 attcgcgact tgcttgaaaa ggaatggtta gttcctagaa aggttcgaca ctttttgcgt
    13501 atacgtaaaa acgtttggat caatcaacaa cctgctttgt tccatcatga agtaaagggc
    13561 aatgaccaga tcacattgtt attcgaagaa acagattata attatcaaga agttcaatta
    13621 ggagatgcga agcacatccg tgttttatat gaagatgagc atttgattat agtggataag
    13681 cctgcaggaa tgaagactca tccaaatgaa ccgacagaaa acagtacctt actcaatcat
    13741 ttagctgctt atctcgacac taaacagcaa cgaccttacg ttgtccaccg cttggacaaa
    13801 gaaacaagcg gtgccgtttt atttgcaaaa aatccatttg tcttgccgat acttggaaga
    13861 atgttggaac aaaaaatgat ctatcggaga tatcaagcca tcgtatgggg agaaattgcc
    13921 aaagatatga ccatcaccaa taaaattgga cgagatcgcc atgatcggag aaaaagggtc
    13981 gtagatgata aaaatggtca gagcgccttg acacatgtca ctgtcaacaa agtactttct
    14041 gaacaaacac aagtctattg tgttttggaa accggacgta cacaccagat cagagttcat
    14101 ttagctcata agggacatcc gatcgttgga gaccctctct accaaaatag aacagctgaa
    14161 cgactgatgc tccatgcatt agaactgcac atgatccatc catttactca agaaaaaata
    14221 gtcggtaaag ctttacctgg attatgggaa tagaaataaa aaagaacaga gtcttatcag
    14281 cagataatga taagactctg ttctttttgt gtaatttgtc tgcttacttt tgatgttcgt
    14341 gttctgctaa cagcaaacta ccgataatcc cactttcatt cggcagtccc caacgaacga
    14401 tatattcact tacttgaggt gtttccatat aaccacccat caattcaaca aattgctgac
    14461 ggattttttg aaggagatga tcctgattca ttaccccacc gcctaaaata atcttttcag
    14521 gtgctaacgt caacgtatag ttgatcagtg cttgagcaat ataataagcc tgaatagccc
    14581 aaacaggatg atcttgcggc agatcttgcc ctttgatccc tgtgcgtgcc tcaatagacg
    14641 gtcctgctgc taatccttct aaacaatctt tatgataagg acaagtacct tcataatggt
    14701 catctggatg gcgtttcacc caaatatgtc ccatttcagg atgtgcgatc cctgaaaaaa
    14761 tttcttcatt gatcacgaca cctgcaccga ttcctgttcc cacggtaagg taaatacaac
    14821 tattttttcc ttgtgctgct ccttgcttca attccccata tgcagcagca ttgacatccg
    14881 ttgtccaagc agcaggaata ttgtagcgtt ccttgattgt gccaagaaaa tcgaattgtg
    14941 cccacccttt tttgggagtt gccaaaacgt atccgtaatt atcttttgtt ttgtcgatac
    15001 caatcgggcc gaatgagcca atcccgattc ccttcaattc ataacgatca aaaaattcaa
    15061 atacctgatc caatgtttct tctggtgttg tggtagggat acttaatttt tctaaaattt
    15121 ctccaccatc tgaaaccgca cacacaaatt ttgttccgcc tgcttctatt ccgccataca
    15181 ttcgttttcc tcctcgaatc cgattgtccg aatctcataa ggttggatta ttgggacaac
    15241 cgcttgcttc gttggttctt caagcagatt caatatgaca gtttttccat tgtctttagt
    15301 tatttccaat ttttctaaat gactagacat attgaaacct cttactacta ctttatcact
    15361 aaacttgctg cgtttcaaag ctgtaattgc aaacgtttca ctttttatcg ttaaatattg
    15421 ttgttttgaa atcaatgttc cttcatgatg tttgatttgt tgagtggaaa aaggaatctg
    15481 tgctgcgtat gcatgcaaat aagtagagaa tttttcttct ggtccatgta gttcaatgct
    15541 gtaattgaac cgatgctcac ctagacactg tgcttctggt gttggaaaat atccccaatc
    15601 gcctagttct cccacactgc gaagtagcgt cactgcgatt tgtccgtctt ctgttacttc
    15661 atattcattc aaaccaaagt ttccgactgt tacaccgtac tcttcatcat gaatattgac
    15721 aaatgcttgc tgatgttgcg gattcgtggg gttttcccaa cttttagaca catggtttgg
    15781 gcgcttcacc acttcaaaaa tactatctgc ttcgtgatgt tccacatgta actttgtagg
    15841 aaacaagaca cgtaagcgat gatctttcat ctgattgtcg agagtcgttt caaagtcgat
    15901 ttttttacta tcttttctga ttgtcatgat tgtttttatt tgaaggattc gtttttcttt
    15961 ggatcgttct gcttttcgat aacgaaattc cacaaccatc tgctgttctt tttctaaacg
    16021 ctcgtctgca gaaacaggga tttccatttc ctgtagaata cttatttttg ctatttccgg
    16081 tgtatctact aatactttta cttcagagtt ttctttgtta ctggatagga ttggctgatc
    16141 acagaaaggt tgtttaaaga tatactcatt tcctatatcg ccgatatctt caaaaatatg
    16201 cagattattc aactttcgtt tgtttttgcg atcttccatt gtaatggtac catttttttc
    16261 aatcgtcaaa tgtagagggc cgttttcgat gattcgtcca ctctgatgaa tcattgtttc
    16321 cttttcttct gctttcgctt ccccttctac aagcgcgaat gtttcccaag aaaatgctgg
    16381 catgttttct aatggtaaag tcactttgat cattcttgcc atatatggca cacggaaacg
    16441 gtctttcggc aaatcataac caaattggac agtttcttca gataaaatag ctggtatagt
    16501 ggccccactt tttgtttcta catgatactt acgtttaggc aaattttcta actcttgata
    16561 tagttgttca ggtattcctt cagcgaatct ttttctttct aattcgatgg ttatttctgc
    16621 ttcaccagtt ttatccattc ctgcagtatt tacaatgaca aatggaaagc tgtcttttgg
    16681 gaatccggtc gtatcgattg ctgcggtcag ctgttctaag gaatcttgtg caagatactt
    16741 tccaacttca tctgcttttt caaaacgagg tatcatttcc cgatggacag aatcgactga
    16801 acaaccgcaa atactgtcgt gaggatgatt ttgcattaat gtcttccaag cataatctaa
    16861 ttgatcatgg ggatagtttc cactaacttc ataagccatc gtcgcaagtg gttctgtaat
    16921 attttctagc tgtcgctgga ccttggtatt ccattgtttt aagtaaaccc gagcagatgc
    16981 tgtattggct aaagtatacc agccatccgt ttcttgactt gtcaattctc cttctactga
    17041 gcctaagtct tctggcacat ctttttgaac tgcttccaga tagtcagtaa aattagaatg
    17101 aataaactcg taatctggaa acagttcatt tgcgagatgg attgcttttg atatatcttt
    17161 ttgtaccggt tgatgatcaa caccattcat catcaataag tggttcgtag aggcatattg
    17221 ttctgcatcc gctaattttt gcttccaaaa ggcaagagct gcttcttttt ctactggaat
    17281 ctcattcccg ttgctatacc aattggcaaa tagcagacca aagattgctg tttgatcagg
    17341 tcctttccac cacatctctg agtattggga agaatagttt tctgcttcca aaacttgatt
    17401 atcaaagcca attggtttta caccacggcc aaacgctgct gctgaaatac cagcttgttt
    17461 cattaattga ggcgtctgtc ccatatttcc aaatgtatcg ggaaaatagc ctaacatgac
    17521 aggggttccc catttacggc tttcttccat gccaatcagc atatttctca cgttcgattc
    17581 cgagctgata agaaaatcat cttgcaagat ataaaatggt ccaattctca attttccttc
    17641 attgattgct tgttggattg cctgtctttt ttctggcctt acttgaagat agtcatctaa
    17701 aataatcgtt tgcccatcca aatgaaaact gttgaaggac ggatcaactt caaatagctc
    17761 tagtagatcg tctatcaatt caatcagtcg catgtgatgc tgttcgtaag ccatgtacca
    17821 ttcacgatcc cagtggctat gagaaatgat atatactttc tttttcattt acgatacctc
    17881 ctacttttct actctaatat caaagtaatc catgactaat tcgcaaaaca tcatatttgc
    17941 ccaagaaaac cattctctcg tataattttc aggattgttc acatcgaatc cttcgtgcat
    18001 aaggtgtgtt cctgcgtcag ttgctacaag taaattcaat atccgctctt tttcagattt
    18061 gtctgacgtt gtcattcctt ccatagctaa tgcaatcggc caaacgtatt tttctggcgt
    18121 atgggaggat ccaattcctt ttgcgtactt tccttcatag aaatatggat tttctttact
    18181 caacaatgtt cttcttgttt gcagataatc ttcctcttgt tcagaacaat atcccaagta
    18241 aggtgcggcg atcagattgg gcacattact gtcatccatg actgtggcgt ttcccattcc
    18301 atctacttca tatgcataaa tcgtttgccc tttttgatta accgttcgtc cgtgattctg
    18361 aatcccctct tggatctctt tcttcaattt ctgtgtttgc tgtacaattt ctttatcatc
    18421 gagtatcgtt tgaaaaattt cttccacata acctaacacg accaccgcaa acatattgga
    18481 tggtaccaga tatccataac ggcaagtatc atcgctagga cggaaaccag accatgtcat
    18541 tcctgtaaaa gctgtttttg cacctcgccc gtcattgatc agcgtatctt cttttcttgt
    18601 cgtgtctcgt acaaatgtat aaggagagtg ttcatgatct tgctctgttt tgaacacgtc
    18661 caagattttt ttgatacctt caataaaaga ttgattgaat tgatcagttt ttcctgtatt
    18721 tttgtaaaga agataggcca actgtattgg gtagcataaa gaatcaattt catattttct
    18781 ttcccagatc caatcgttca tttttgtatg atctgtttgg tggccagcac cgttagcttc
    18841 ttcgttgaag gcattcgcat atggatcgat attgatataa aaaaattgct ttttgactaa
    18901 cccgctgatc atgtcagcta attcctcatc ttcttttgca atcactaaat aaggacgaac
    18961 ttgtgcagta gagtctctta accacatcgc tggaatgtct cccgtcaata aaaaggtggt
    19021 tccgtcattt tgctttttga ctgttgtcaa cagagtattg gcaaatgctg ctgtaaaatt
    19081 ctttgcccag tccgcatgga tatccccaca tttttgtgtg atatcttcca taaatttctg
    19141 caccgatttt ggtatatctt tataagccac taataaaacc ttctttctat ttgatatatc
    19201 tttaaagatt atacttcata cagccaatta tgtctacgct ttcaaaaaat atctttttat
    19261 ttactttttc atccattatg gtattatttg aatatgatat atcattaaga ggtgtttata
    19321 tgaatcaacc attgtaccag acaatattta atgatttgca aacggctatt ttaactggtg
    19381 aattacctgt agacagccaa ctaccaactg aaaaagaact ttcacgtacc tataaagtaa
    19441 gcagaatcac ttccaaacgt gcattgactg aattagagca attaggactt atttatcgcg
    19501 ttcgcgggaa aggcagtttc gttaaatccg cttctccgga acagattctt gcttctccta
    19561 caaaaacacg acggattctg tttcttcttc catatgtcgc tgatttgtct gttggtgatt
    19621 ttagcaaggg attgaaccct gtgatgcaag ataaacagat tgatgtcatg atgacgacgc
    19681 ttgactttct acaaaataaa acagcaaaag agctaatcaa tgaatttgac ggattgattt
    19741 attatgctct tgatacagaa caacatcttg acttactttt tgagctttca ttgaaagaat
    19801 tccctgtcgt catattagat aaaaaaatct atgagttacc ttttcctacc gttctttccg
    19861 ataatttcca aggcggtgcg cttgctgcct cttatttgat cgaacaggga cattcaaaaa
    19921 tcgcttattt gtttggtacg cagactcatc cacaatccgt tcgacagcgt taccttggtt
    19981 accttgaagc attggacaaa gctggtttga ctttccacac ttcactggat gacaaaagcg
    20041 cgacattaga tgaattgctt tcttatatca ctaagtatga agtaactgct tttgtttgtg
    20101 aaaacgatgt tgtcgctatc cagaccatga atctcttgag acagcatggt tatcatatcc
    20161 ctgaagattt ttcaattatc ggatttgatg atatccaagc agcagctctt gttgacccgc
    20221 cactgacaac catcgcacaa gattttgaac agttaggtaa gcttgcggga gatgctttgg
    20281 tcacttggtt agagacaaaa gatcgaccat ccgatagtaa atttccagtt tcattcatca
    20341 aaagacaatc aacaaaggag aaataaaatg aatatcggtc aaatagatac tagacatgca
    20401 acagcgaatc agccaaattt ctctaatggg aactgccagc cttatactgg cgttcctttt
    20461 gggatgaatt attttgcacc gcaaactacg gatcaaaacg gcagctggtg gtttcatcct
    20521 gatgaccgcg tttttcaagg ctaccgtctt acccatcaac caagtccttg gatgggtgat
    20581 ttcagtcata tgctgtttac gccgatcaat ggaaaactac aggagaacac ccttttccat
    20641 gctcaaagtt cttaccgccc agaagaaagt atattttgcc cgactcatct gtcgatctgt
    20701 caattacgtt atggcatctg ttctacattg atacctagca tgtatggcgg aatcttatct
    20761 attgattaca gcaaaaatga cagcggttta cttttatcct ttccgggacg ccatcagctc
    20821 tttgttatag atccttacac agtggaagga aaaatcacac attttgctgg ttcagaagat
    20881 cctgattttt ctttttattt tatattgaga tttgaatatc ctttaacaat tgatgagtta
    20941 gctgtttcca acaaaaatag cgactcgatc ccgttgtatt ttgaacagac aaaaaaacaa
    21001 actatccgat ttgggacatc ttttatcagc tcggaacaag cagcttataa cctggaacta
    21061 gaacaaaacc aaagcaaaga gacttacctt gctcacagtc aagaacaatg gcagtcctat
    21121 tttgatcgta tacagctcac acaccatgat caacaacaat tgcggacgtt ttatcataat
    21181 ttatatcggg tgtttttatt tccacaaaca ttttatgaaa ttgatcctag tgggcaaaaa
    21241 atccactatg atacgactag tcgatctata aaacctggtg ttctctatac aaataatggg
    21301 ttttgggata cgtataaaac ggtttatcct ctttattctt tgattgctgt agaaaaatac
    21361 gaagaaatgc tggaaggttt tttgaacagc tatagagaaa gtggctattt gccaaaatgg
    21421 ctgtctcctg atgaacgtgg acttatgcct gggacattga tcgatgcggt cattgcagat
    21481 gctgctgtaa aagatatccg acgagacttg atgcctgaat ttctaacggc aatggaaaaa
    21541 ggtgctacgg ttcaaagcaa acatccaaat tatggacgcc aaggaacaga agattacttg
    21601 acatatggct atgttcctaa tcactaccac gaatcagtca atcacacatt agactatgct
    21661 tatagcgatt tttgtattag ccaagttgcc aaggtactag aagaagagga aatgacgaag
    21721 aaatatcagc agcaatcatt gaactatcaa aatatctttg actatgagtc aggatttatg
    21781 cgttcaaaag ataaagatgg caagttcaga gaaccatttt cagctaatcg ttggggacaa
    21841 gattatgcag aaggaagtgc ttggcaaagc agctttgctg tttatcacga tttttcagga
    21901 ctgatcggaa aatatggtgg cagtgagaga tataaagaaa aactcattga gctgtgcaat
    21961 cagcccccaa cgtttgaccc aggaggatat ggttttgaga tccacgaaat gagtgagatg
    22021 gccgcgatag aatttggaca attagctatt tcaaaccagc caagttttca ctatccttac
    22081 ttgttcagtt atataggaca acctgaaatg gcacagccac tgcttaaaca gttgatgact
    22141 cagaccttcg acgattctcc aatgggttac cctggagatg aggacaatgg aagtatggcc
    22201 gcttggtata tcttcaatag tctgggattt tatcctgtca cgcctggaac cggtgaatat
    22261 gttatcggta tgccattaat tgacaaggct gttttgcttt tgtcaaatgg aaaggaattg
    22321 acgattgaag caacgcctaa tcggccgcag caacaattta tccataaaat caaacggatg
    22381 gacaaagaat atacgaaatt atattttacc catcaagatt tgctgaaagg cggcacgatc
    22441 tcttaccaat tagggatcat ccctcaccct aagaaatatc aagcaaaaga tttgcctttt
    22501 tccttatcaa actataatta acaagaggtt gtgaaaaagc tgtcctgcat caagtaataa
    22561 gaacagccaa gcaaaaatag atcctcatat ttttcgtttg gttggtctta ttaccgcaga
    22621 tgcaagcttt tgaacaccgt ttattgagag gttgtgaaat cagcgttctg acctgagtat
    22681 tagagagaca aatggcaaaa tagttcctca tattttgcca ttttttgagc taataccgag
    22741 ggtcagctga ttgaacaccg tttagtggag aggtcgtgaa aaagctgttt agtttcgagt
    22801 attaagaaaa attttgtgaa aatggctttc cacatttttg cataaatttg gcttaatacc
    22861 gagaaactgg cttttgaact ccgtttatcg agaggtcatg aaaaagctgc cttgcctcaa
    22921 gtaataagaa cagcccaaaa ttagatttct agacaatttt ttataagtta tttcattaca
    22981 ttggcttttt cttttatgat tctccactgt tttctcggta ctctttaggg gatctgccga
    23041 cgattttctt gaataattta gagaaatacg tggtgttgtt gtagccaatc tcacttgcta
    23101 tctcattgat gttttgatta ctgtgcaaaa gcagttgttt cgctttttcc atacgtaaat
    23161 ggttcaaata agctgaaaaa ctttttttcg tttctttttt gaacagctgt cctagataca
    23221 tgacatttaa atgcaggttt tctgacactt ctttgagtgt aagtggttct tgataatgct
    23281 ggtgcaggat tacgattact ttttccacat tgtcactgta ctttttctgt ctctgatgct
    23341 ttttgatgaa tgacagaagt agctgctgaa gttcttgcac cgtttttgcc tgatggattt
    23401 tttcgattcc ttgtaaatat tcctcatctt ccagatcaat caattcccgt tgaatctcca
    23461 taaacagtaa aaaactggaa tatcgtatat cttccggcat catggctgct aactgaaact
    23521 gttcaaaaaa atcagtgatc attttttgag cgacttccaa ttgtttattt tgcagcacgc
    23581 gtctgaaact tgaaaaatcg atggtttgat cttttgtttc tgcttgtact gcgtaaaaaa
    23641 catgctcgtc tttatctcca taaaattgat gcaactgtag attgtctttc gcttgttgga
    23701 atgtttcagg gattttgtcc aaagaatctg tctcttcccc gatactgatc agccattcct
    23761 gatcaggaaa gttttcttga acaaaacaac agaagccatc aacttcatct gcctccaaca
    23821 acaacgtgat caatacacgg tctccgtaat ttcgttgata gtaaaagatt tgttgccgtt
    23881 cttgcaacca tctattgata agagtatcat gagttcttgc cagttgaatc aatactatcc
    23941 tatgctgctt ctcacttatt tgtcctatta tttcttcttc acttatttca tccaattctt
    24001 cattcaacca ttgactgaat aacagttctt gatagatttc ttgttgattt ttttgttcat
    24061 tttgctgatc taaccgtgtt ttgattttct tgaggctttc aaccaattcg aacttattca
    24121 cgggtttcat caaataattc acggcgccta attgcatacc tcctttcaaa taatcgaatt
    24181 cttggtatcc tgataaaatc ataaattcaa aattatgacg ttctttttga gccgcttcaa
    24241 taaattccag tccatttatt tctggcattg tcacatccgt tactactaaa tcaatctgtc
    24301 gctgttcaag tacagctagt ccttctttgg cattctctgc tgttgctact atttgaaagc
    24361 ctaaactttg ccaatcaatt atctttttta acccattcaa aatcatgtat tcatcatcta
    24421 ccaataacac ttgatacacg ctcatccctc cttgaatgta atagaaacgg tcagtccacc
    24481 tgtctcatta ttctcaagcg acatataata actgctgccg aagctggcac gtaaacgttc
    24541 attcacgttt tgcagaccga tagaaccgtg aagttccact ttaggatgag acagtttttc
    24601 ttcaatctgt tttagccgtt tttcagtcat cccttttcca ttatctttga tgatgatcct
    24661 tacccgtttg ccttcctgca agactttgac acttagcgca ttatcgaatc gagtaaaatc
    24721 aataccgtgt ttaaaataat tttcaactaa tggctggatc acaaatttag gtacttcgat
    24781 cttttccaaa tcaggatcga tcataaaatg ataggctact cgattaggat aacgcatttg
    24841 atacaaatag acatattttt cgcaaaagtc cagttcttct tttaaagtga tcgttttctc
    24901 ttgattcgta ttgtttcgaa gcaaagcgga aaacgcatac acaacatctg ctaattcttc
    24961 acttccttcg ctgattgcat acattcggat atattctaat gtattatata aaaagtgtga
    25021 attgatctgt gcttgaagcg cccgcatatg tgcgtcttgc tgtttgattt ctaatttgta
    25081 gatgtcttct acaaactgat tgatgctatc caacatttcg ttgataccga ttgaaagttc
    25141 cttcagttca tattccgtgt ttgttatgtc gatccgcgtc tctaaattcc ctgctgctgt
    25201 ttgatccatg gcctgcatga cgatagatac ttgctgtgaa tatcgtttga atgttttata
    25261 aagaaagata agcagtatat tgtctaaaat aatgatacca atgatcagcg gggcaagatt
    25321 ttttcccgtt tcgattttta cacttttttt atctagtaca gctaatatac gataatcatc
    25381 agacagttct tggtgttgaa caaaattgtt tttttccaga tcgtcaatcg gcaattgtga
    25441 atttttcttc agactctctg tgatgatcat ttcttgtctt tcaaacgttt ctttagctat
    25501 tttattatca tggaatgtgt atagtcgatt tgtcgttcca gaaatcatgt ataaagagag
    25561 tccattgaat gacgttagat tttctaaaat catatctagt tctttctttt gaaaaccaat
    25621 aaaaatactt cctgactcag ctcctccttg tgtgataggc aacttgatat aaaaggcgtc
    25681 tagtaatttt ggtgtcccct tttttatttc cccacctttg ttttctctag aagattcata
    25741 atactccggt agttgattca acacgatata tatcgcactt agttcttcat aatttgcgta
    25801 gagatctttg atttgatttg ggaaagaaac taattcatcg gtactgtttt gctgttcgta
    25861 tacatacatc agatactgat ctatgggttt tgttaagtag gtcgtcacat tttcgatctt
    25921 ttcggaattc cctacaagct ggtttttgat gatcctactc aaggttgttt tatcattcag
    25981 cattctgctt gtgctttgaa cggcatcact tgtcatgttt tctgcctgtt tttgattttg
    26041 ttcccaagta tggatactaa aaataacggt agcagtcatc gttacaccga tcatgataac
    26101 cgcgtatttc cgcaacagac gatttagtaa ggttcctttt gaaaatagaa tccgaaactt
    26161 attcataact ttccaactgt ttatctaatt cttcacgcca atgtgttgtc atcgtaccat
    26221 tccaaaccgt caaaagacca atgacaccaa acaggatcaa tcctttgaat tgccaagtca
    26281 cgcctaaaat tataccgcta ccaataatta tttttagaaa agacgaaaag ctggaaaaaa
    26341 ccgaaataaa acttagcttt agcagattag gaacagagat cgtatattca ctatctaaaa
    26401 tcatactgta ttgataagta gcaaataggc agagcaaggc aaaaagtaaa ataaaatcaa
    26461 tagctaaaaa aatgagccct ttgatttgaa cagataaata taaattgtaa ctcaataaag
    26521 cagtaccaga taaaaataaa ctaaatagca gattgcctct taaaaaattt cttttatata
    26581 tattccagcc tctttttatt gtgatttctt tatactcgaa tccgtacaaa taaaatagtt
    26641 cattgactgt tttccatgcc gcacctaatc caaagatgat cccgccggca aatgtatata
    26701 tccagaaata caaggttagt tttataatga cccagatccg gataaataac tcttccaaaa
    26761 ccttccctaa catatcttct cctccaaaca ttaagtttat actttctcat tatacaaaaa
    26821 aaggttgggc aatcagcacc ttgacctgat tattagagaa acaaacggaa aaatagttcc
    26881 tcatattttg tcgttttgtg agcgaatacc gagggttagc tgattgaaca ccgtttacca
    26941 agagtttgtg aaatagccgc ttagtttcga gtagtaagaa aaattttgtg aatatggctt
    27001 tccacatttt tacatat
//
LOCUS       DACCXO010000028        26838 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.029, whole genome shotgun sequence.
ACCESSION   DACCXO010000028 DACCXO010000000
VERSION     DACCXO010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.029"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            84..761
                     /locus_tag="IVY70_001112"
                     /pseudo
     CDS             84..761
                     /locus_tag="IVY70_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1018..2217
                     /locus_tag="IVY70_001113"
     CDS             1018..2217
                     /locus_tag="IVY70_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7958254.1"
                     /translation="MLFTSGPDRAFERLMQEKPGFDHYDGGGADAPEDCGTCRFYRPD
                     WKYQFCQFAECPYQPGKLTDYAAVKFTVKGANNAMAVFRVEKNKGYTVMSNHHLRNKN
                     LTLKAKGLLSQMLSLPEDWDYTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDA
                     KGKMADMVYTIYEQPVLENPVLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLS
                     KKEKIITDGQSTHSIPIPSPTPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEY
                     AYLIQDRSIDREQLDEIVDLMLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRF
                     VLDCLRENTTKVRNIKQYLRAMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYS
                     CGEGESL"
     gene            2239..2451
                     /locus_tag="IVY70_001114"
     CDS             2239..2451
                     /locus_tag="IVY70_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP7958255.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            2526..3002
                     /locus_tag="IVY70_001115"
     CDS             2526..3002
                     /locus_tag="IVY70_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAP7958256.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            2999..4807
                     /locus_tag="IVY70_001116"
     CDS             2999..4807
                     /locus_tag="IVY70_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP7958257.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            4884..5099
                     /locus_tag="IVY70_001117"
     CDS             4884..5099
                     /locus_tag="IVY70_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer protein"
                     /protein_id="HAP7958258.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            5231..6052
                     /locus_tag="IVY70_001118"
     CDS             5231..6052
                     /locus_tag="IVY70_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958259.1"
                     /translation="MDGITGNLSGLFDTVNAKVGEIAADVGRTPQAWNSGIFNMLHSL
                     SETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIFKSAFAILIVTNTWN
                     IVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSVGALLGLWFQTLVVG
                     LTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMGQGYLKSLLALGFQA
                     FLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKTGSISKAVFGAH"
     gene            6083..6625
                     /locus_tag="IVY70_001119"
     CDS             6083..6625
                     /locus_tag="IVY70_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HAP7958260.1"
                     /translation="MFISTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVE
                     RLASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTR
                     GNAEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFAR
                     QKTPGWDVWGNEVECDLILT"
     gene            6639..7061
                     /locus_tag="IVY70_001120"
     CDS             6639..7061
                     /locus_tag="IVY70_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP7958261.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            6991..9390
                     /locus_tag="IVY70_001121"
     CDS             6991..9390
                     /locus_tag="IVY70_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP7958262.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            9422..11413
                     /locus_tag="IVY70_001122"
     CDS             9422..11413
                     /locus_tag="IVY70_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP7958263.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            11436..11687
                     /locus_tag="IVY70_001123"
     CDS             11436..11687
                     /locus_tag="IVY70_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HAP7958264.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            11677..12906
                     /locus_tag="IVY70_001124"
     CDS             11677..12906
                     /locus_tag="IVY70_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAP7958265.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            12903..14984
                     /gene="topB"
                     /locus_tag="IVY70_001125"
     CDS             12903..14984
                     /gene="topB"
                     /locus_tag="IVY70_001125"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7958266.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            15133..19053
                     /locus_tag="IVY70_001126"
     CDS             15133..19053
                     /locus_tag="IVY70_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAP7958267.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            19054..19998
                     /locus_tag="IVY70_001127"
     CDS             19054..19998
                     /locus_tag="IVY70_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958268.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            20002..20205
                     /locus_tag="IVY70_001128"
     CDS             20002..20205
                     /locus_tag="IVY70_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958269.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(20505..20741)
                     /locus_tag="IVY70_001129"
     CDS             complement(20505..20741)
                     /locus_tag="IVY70_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7958270.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(20924..21295)
                     /locus_tag="IVY70_001130"
     CDS             complement(20924..21295)
                     /locus_tag="IVY70_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958271.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(21292..22620)
                     /locus_tag="IVY70_001131"
     CDS             complement(21292..22620)
                     /locus_tag="IVY70_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7958272.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(22581..22910)
                     /locus_tag="IVY70_001132"
     CDS             complement(22581..22910)
                     /locus_tag="IVY70_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP7958273.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(23168..23538)
                     /locus_tag="IVY70_001133"
                     /pseudo
     CDS             complement(23168..23538)
                     /locus_tag="IVY70_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            23683..23868
                     /locus_tag="IVY70_001134"
     CDS             23683..23868
                     /locus_tag="IVY70_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7958274.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            24380..24598
                     /locus_tag="IVY70_001135"
     CDS             24380..24598
                     /locus_tag="IVY70_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958275.1"
                     /translation="MRQERPEGVHTGPACVKSHTARCIRKKEAAHFAEAWGVPNKQNE
                     NAAGGQVVDQNIRKGYSDPLLASIIGMF"
     gene            24811..25470
                     /locus_tag="IVY70_001136"
     CDS             24811..25470
                     /locus_tag="IVY70_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HAP7958276.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            25473..26816
                     /gene="vanS"
                     /locus_tag="IVY70_001137"
     CDS             25473..26816
                     /gene="vanS"
                     /locus_tag="IVY70_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP7958277.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
BASE COUNT         6727 a         7044 c         7283 g         5784 t
ORIGIN      
        1 agtaatacat tagatatttt atataaaaac accattttca ctaatctcta atcaatttac
       61 tgaatcgaaa ggaaccgggt agaatggata agaaagtttt aataaagagg ttctctgaag
      121 ctattgaaaa aggaaatgca gcaatatttg caggtgcagg attatcgatg tcacaaggat
      181 atgtaagttg gccggaacta cttaatgatc ctgcaacaga aatcgggttg gattctaaaa
      241 aagaaacaga cttagttact ctggctcaat attataaaaa tgaaaatggt ggatcaagag
      301 gaattttgaa tcagatactt atggataatt ttggtgaaga attagaaata tctgaaaatc
      361 atcgaatact agcaagttta cctattgaaa cttattggac aactaattat gatcatctaa
      421 tagaaaaatc aatccgagaa gcttataaaa accctcaggt taagaaaaat tatactcagt
      481 tagcaacaac taatccaaat gtggatacaa ttgtttataa aatgcatggt gatatagatg
      541 atgtcagttc gacagtaata acaagagatg attatgaaaa atatgatgat gattcgtacg
      601 cgttatttaa agaaacttta aaaggagatt tgttaacaaa aacttttctc tttttaggat
      661 ttagttttac agaccctaat ttagaaagaa ttttatcaga tataagatgg gttttgagag
      721 aaaaccaaag acctcattat tgtataatga gaaaaatatt atagaaaaaa ttttaggtta
      781 tcgctgaaag ccccggaaat acgggcattt cgcaggatat gagaactcaa tttactacca
      841 atttactact ttcggattga gccgcatata tgctgaacca tactgctaat acccgccacc
      901 ccaaatacgg cacatcataa attgaatacg acaccgtaaa cgcggcgttc ctctttcccg
      961 tcatgggaga acaggacgcc gcttttttat gcccaaatag agaaaggagg cgacgccatg
     1021 ctatttactt ccggccctga ccgggcgttt gaacggctga tgcaggaaaa gcccggtttc
     1081 gatcactacg acggcggcgg ggcggacgcg ccggaggatt gcggcacctg ccgattttac
     1141 cgcccggatt ggaaatatca attctgccag tttgcagagt gtccctatca gccgggcaag
     1201 ctgaccgact atgcagcggt caaattcaca gtgaaaggag cgaacaacgc tatggcagtt
     1261 ttccgtgtgg aaaagaacaa aggctatacg gtgatgtcaa atcaccacct gcggaataag
     1321 aacctgacct taaaggccaa gggcctgctg tcgcagatgt tgtccttgcc ggaggattgg
     1381 gactataccc taaaaggact gtcctatatc aaccgggaaa gcatcgacgc gatcaggaca
     1441 gcggtatggg agcttgaaaa agctggatat atccggcgtg agcagggccg tgacgcaaaa
     1501 ggcaaaatgg ccgatatggt ctataccatc tacgaacagc cggtattgga aaacccggta
     1561 ttggaaaatc caacatcgga taacccggtg ttggaaaatc cgacatcgga taatccggcg
     1621 tcggaaaatc caacgcaatt aaataaagaa gtacaaagaa ctgacttatc aaaaaaagaa
     1681 aaaataatta cagatggaca aagtacccat tccattccta tcccatcccc gacccctgcc
     1741 cctatggagg gcgaggcagc ggctacgccg ccggaacgga aaaggacagg aaaggatgag
     1801 gctgtacgga tttatcggga aatcattttg gagaacatcg agtatgccta tctcattcag
     1861 gacaggagca ttgaccgtga acagcttgac gaaatcgtag acctgatgct ggaaaccgtc
     1921 tgcaccgccc gaaagacaat ccgtgtcgcc ggggacgact accctgccga gctggtgaaa
     1981 tccaagtaca tgaagctgac cggcgaacat atccgctttg tccttgactg tctgcgggag
     2041 aacacaacga aagtccgcaa tatcaaacaa tacctgcggg ctatgctgtt caacgccccc
     2101 agcaccattt ccaactacta cacggccctt gtcgcgcacg acatggcaca gcccgattgg
     2161 ggaaagccta aatccggcct gccggactat tcctgcggcg agggcgaaag cctgtaacga
     2221 gaaaaggagg acgaccctat gaagcagggc gcattgattt ttgacgagcg aacagaccgt
     2281 tacgacatcc gctttgatct ggccgactat tacggcggcc tgcattgcgg ggagtgtttc
     2341 gacgtgatga tcggcggcaa atggaggccc acccgcattg aaatggccga aaactggtat
     2401 cttgtcggca tccgggccga cgacctttcc ggcctgcggg tgcggatttg acctatatct
     2461 acctgcggcg tctggtggtt ccgccttaga cgccgctttt gactttctga caaggaggga
     2521 tgcgattgca ggaagaagtt actcaaaaga ccattgccct gtccatgaaa acgggcaagc
     2581 tcacggctca agtgctgcaa gcggcgctga aaaagtacct gcaacaccgg gccaagggca
     2641 aaaacacgct gcaccacggc cagcagagcc taaagcagct taaaaagcat ggggctgatc
     2701 tctccaacat tgagatcacc gaggccaaca ttggagcgtt caagccatgc gccaagaaat
     2761 acggcgtgga ttttacctta cgaaaagata agaccaccca gccgccccac tacgttgtga
     2821 ttttcaaggc caaggatgca gataaccttg atctggcgtt tcgggagttt accgccaaga
     2881 cgctttccaa ggagcagcgg ccctctatcc gcaaggtttt gtccgctgtg aagcaaaagg
     2941 cggcggccca gaccaagcag cgggccaagg agaaaatcaa ggaaaggggg ctggaactgt
     3001 gaagcctgac gtaaagaaat tgctgatcct gaacctcccg tatctgattt ttgtgtacct
     3061 gtttgcgaaa tgcggggagg cgtaccggct ggccgctggt gctgacctgt cggggaagct
     3121 cctgcatttt gcggatggtt ttgcggcggc ttttgccaac ccgctgccaa gcctgcacct
     3181 gtttgacctg tgtatcggta tcgccggtgc cgtgatcgtg cggctggtgg tgtactgcaa
     3241 gagcaagaac gtgaagaaat accgcaaagg catggagtat ggcagcgccc gctgggggac
     3301 ggccaaagac atagcgcctt acatcgaccc caagtttgaa aataatatcc tgctcaccca
     3361 aacggaacgg cttaccatga ccggcaggcc caaagacccc aagacagcgc ggaataaaaa
     3421 cgtgctggtg atcggtggca gcggttcagg gaaaacgaga tttttcgtga aacccaacct
     3481 gatgcaatgc tttccgacca cggactatcc gacttcgttt gtggtgacag atccgaaagg
     3541 cactttggtt ctggaaaccg gcaggatgtt ccagcaggcg ggctaccgca ttaaaatcct
     3601 gaataccatt aacttttcca agtccatgaa gtacaacccc tttgtctaca tccattcgga
     3661 aaaggacgtg ctaaagctgg taaataccct gatttccaac accaagggcg agggggaaaa
     3721 gtcagcggag gatttttggg tgaagtcgga acggttgttt tattcggccc tgatcggcta
     3781 tatctggtac gaggccccag cggaggaaat gaacttctca acgctgctgg aaatgataaa
     3841 cgccagtgaa gcccgcgagg acgacccgga gtttcaaagc cccgtcgatc tcatgtttga
     3901 gcgtttggag cagaaagacc ccgaccattt tgcggtgcgc cagtacaaga aattcctgct
     3961 gtccgcaggc aagacccgtt cttccatcct gatttcatgc ggtgccagac ttgcgccctt
     4021 tgacatccgg gaggtacgcg agctgatgga ggacgacgaa atggagctgg acaccatcgg
     4081 ggacgaaaag acggtgctgt atctcattat gagcgatacg gacacgacct ttaatttcat
     4141 tctggctatg ctccaaagcc agctaatcaa cctgctttgt gaccgggctg atgatgtgta
     4201 cggcgggcgg ctgcctgtcc atgtacgcct gattttggat gaatttgcca acattggcca
     4261 aatacccaat ttcgataaac tcattgccac cattcgaagc cgtgaaatat cggcctctat
     4321 tattttgcag agccagtcgc agctaaaagc gatttacaag gacgccgccg agatcatttc
     4381 ggataactgc gatagtgtgc ttttccttag tgggcgcggc aaaaatgcca aggagatttc
     4441 cgatgcgctg gggaaagaaa cgattgacag tttcaacacc agcgagaacc ggggaagcca
     4501 aacgtcccac ggattgaact atcaaaaatt aggaaaggag ttgatgtccc aagacgaaat
     4561 tgccgtgatg gacggcggca agtgtatctt gcagctacgg ggtgtgcgcc cgtttttatc
     4621 ggagaaattc gatattacca agcatccccg gtacaaatac cttgccgacg ccgacaagaa
     4681 gaataccttt gacgtggaca gatttttgac tatcacgcgc cggaaacggc agcaagtggt
     4741 aacgcaggat gaagtattcg acctgtatga aatcgacctg tcggacgagg acgccgccgc
     4801 tgaataaaca gcggcttttt tcattccaac aacaaaatca aaaaatcatt atgagccgga
     4861 aaccggcaga aagaggtaaa tttatggcat tttttaattc cgcagttact actttgcaga
     4921 ctatcgtgat cggactgggc ggcgctctgt gcgtatgggg cggcatcaat ctgttggagg
     4981 gatacgggca ggacaatccg ggggcaaaat cgcagggtgt caagcagctt gtcgctggcg
     5041 gcggcgtggc cctgatcggt gtcactctgg ttcccctgct gtccgggctg ctgggctgat
     5101 cctggccttt gtccctgtcc aaacccttaa ctaatcgcct ccgcgtcctc ccggccagac
     5161 cgggagggcg cgggaaagga ggtgctaccc gtgtagtgaa atcattgaag aatggataaa
     5221 aggcatcctc atggacggta tcacgggcaa cctgtccggg ctgttcgata cggtaaatgc
     5281 caaagtcgga gaaatcgccg ccgacgtggg acgaaccccg caggcatgga acagcggtat
     5341 tttcaatatg ctgcacagcc tgtcggaaac cgtgatcgtc cccatagccg gggccatact
     5401 cgcccttgtc atgtgctatg agcttatcca gatgataacg gaaaagaaca atatgcacga
     5461 ctttgacagc tccatgttct tccgctggat attcaaatcc gcgtttgcaa tcctgatcgt
     5521 aacgaacaca tggaacatcg tcatgggcgt attcgacgcc acccaaagcg tcgtcaatca
     5581 aagcgccggt gtgattatcg ggcagacatc cattgatttt aacaccctga taccagacct
     5641 tgaaagccaa ttggaggcta tgagcgtggg cgctcttttg gggctgtggt tccaaaccct
     5701 tgttgtgggc ctcaccatga atatcctttc catctgcatt ttccttgtga cctatggccg
     5761 catggtggaa atctatgttg tcacgtcgct ggggcctatc ccccttgcca caatgggcaa
     5821 cgcggaatgg cgcggcatgg gccaagggta tctgaaatcc ctgctggcgc tgggcttcca
     5881 agccttttta atcatggttg tggtgggtat ctacgcggtg ctgatacaga atatcggttc
     5941 cgcttccgac atatccggcg caatctgggg ctgtatgggc tatacggtgc tgctgtgctt
     6001 ctgcctgttc aagaccggca gtatatcaaa ggccgtattc ggggcgcatt gatcccaaaa
     6061 tccaagcata cacaggagga acttgtttat ttcaacgtac aacatcattt acgctgaccc
     6121 gccgtggcgg tatgactgca aaaacgtaca gggcgcagcg gaaaatcact attccaccat
     6181 gagcattgac gagctgtgcg ccctgccggt ggaacggctg gcgtccaaag actgcctgct
     6241 gtttttgtgg gccacattcc ccatgctgcc ggaagctctg cggttaatca aagcgtgggg
     6301 cttttcattc aagaccgtgg cttttgtatg gctgaaacag aaccgtaaaa gccttacatg
     6361 gttttacggg ctgggccgct ggacaagggg aaacgcggaa atctgcctgc tggcaacaca
     6421 cggcaagccg aagcgtcgtt ctgcctctgt ccatcagttt attatcagcc cgattgaaca
     6481 acacagcaaa aagccggatg tgacccgtga aaaaatcgta gagctggcgg gcgatttgcc
     6541 ccgcgtggag ctgtttgcca gacagaaaac ccccggctgg gacgtgtggg ggaacgaggt
     6601 ggaatgtgac cttatcctga cgtgaaagga ggtgccaaat ggcttatgta accgtcccca
     6661 aggacttaac ccatgtgaaa agcaaggttg tgtttgggct taccaaacgg caactgatct
     6721 gtttttccgg ggcgctggtg atcggcgtcc ccttatattt tctcacccgt gactatctta
     6781 ccaacagcgc ggcggccctg ctcatggtat ttgccatgct gccgggcttt ttgcttgcca
     6841 tgtacgagcg tcacggccag ccccttgaag tggtgattat gcagatcatc cagtgctgct
     6901 tcatccagcc aaaggagcgt ccctatcaga ccgacaacac ctacaccgcc cttgtgcggc
     6961 aataccagat ggaacaggag gtaaaggcca ttgtccaaaa aggaaatccc aagagaaacc
     7021 gaaaaaacaa agctgacccg cgcccagcga aaagaaattg acgcggtaat ccgtaaatat
     7081 aagggcgacg gcaggcccca cacggcgcag cagagcatcc cctacgaggt catgtacccg
     7141 gacggcgtgt gccgggtatc gcccggcgtc ttttccaagt gcattgagtt tgcggacatc
     7201 agctatcagc ttgcccagcc ggacacgcag acggctatct ttgaaaaact gtgcgacctg
     7261 tataactatg tggacgcctc cattcatatc cagttttctt tcctcaatcg gaaagttgac
     7321 ccggtgcagt acgccaagag ctttgagatc gcgccgcagg gggacgactt cgacgacatc
     7381 cgggccgagt acaccggcat tctgcaaaag cagcttgcaa acggcaacaa cggcatggta
     7441 aagaccaaat acctgacatt caccatcgag gccgaaagcg tgaaagcggc gcgagccaga
     7501 ttgaagcgca tcggctttga cctgttaggc tatttcaaga gcatgggggc cgtagcccat
     7561 gtgatggacg gctgggaacg gctgaacctg ctgcatggcg tgtaccaccc ggacggcgag
     7621 atattcaatt ttgactggaa gtggctggcc ccgtccggcc tctccaccaa ggattttatt
     7681 gccccgtcct ccctgtgctt cggcaatgca aagacattcg gcatgggcgg caaatacggc
     7741 gcggtaagtt tcctgcaaat cctgtccccg gagctgtcgg acgatatgct ggccgacttc
     7801 ctcaatacgg aaagcggtgt tttagtcaat ctccatgtgc aggccattga acagaccaag
     7861 gcgatcaaga ccatcaagcg caaaattacc gaccttgacg ctatgaagat tgcggagcag
     7921 aaaaaggccg tccgcagcgg ttacgatatg gacattcttc ccagcgacct tgccacctac
     7981 ggcgaggacg ccaagaagct gctgacaaag ttgcagaccc gcaatgaacg gctgttccag
     8041 ttgacgttcc ttgtcctcaa cgtggccgac accaagcaaa agctgaataa cgacgtgttc
     8101 caagccgcag gcgtggccca aaagcataac tgtccccttg tccggctgga ctaccagcag
     8161 gaacagggcc ttgcaagcag cctgccgctg ggcgtcaatc agatcaagat acagcgcagc
     8221 cttacgactt ccagcgtggc cgtgttcgtc ccctttgtga cgcaggagct ttttcagggc
     8281 ggcgcggcca tgtattacgg catcaacgcc aagtcccgca acatgattat gctggacagg
     8341 aagcaggccc gttgccccaa tgccttgaag ctcggcaccc ccggcagcgg caaatccatg
     8401 agctgtaaat ccgagatcgt cagtgtgttc ttaacgacgc ctgacgatat ttttatttca
     8461 gacccggagg ccgaatatta cccccttgtc aaacggctcc acggacaggt aatccggctg
     8521 tcgcctacca gcaaggactt tgtgaacccg ctggacatca acctgaatta ttcggaggac
     8581 gataacccgc tggccctgaa atccgacttt gtgctgtcgt tctgtgagct tgtcatgggc
     8641 ggcaagaacg ggctggaagc cattgaaaag accgtgatag accgggctgt gcgcgtgatc
     8701 taccggccct atctggcaga cccccgcccg gagaatatgc cgatcctgtc cgacctccac
     8761 aaagcgttgt tagatcagca tgtcccggag gctgaccggg tggcgcaggc ccttgacctg
     8821 tatgtgtccg gctccctgaa cgtatttaac caccgtacca acgtggacat cggcaaccgg
     8881 cttgtgtcct ttgacattaa ggagctgggc aagcagctca aaaagctggg gatgctcatt
     8941 gtccaagacc agatatgggg gcgcgtcacg gccaaccgaa gccaaggaaa ggccacatgg
     9001 tattttgccg acgagttcca tttgcttttg aaagaggaac agacggcggc ctattctgcg
     9061 gagatttgga agcggtttag gaaatggggc ggcattccaa caggtgccac ccagaacgtc
     9121 aaagacctgc tttcctcccc ggaaattgaa aatatccttg aaaacagcga cttcattacg
     9181 ctgctcaatc aggcgtccgg ggatagaaaa atccttgcgg aacgcttgaa tttgtccacc
     9241 gaacagcaga agtacatcga caattccgaa cccggcgagg gccttttgat ctttgaaaac
     9301 gtggtgctgc cctttaccaa ccccatcccg cacaacaccc agctttacaa gattatgacg
     9361 acccgcttaa acgaggtggc gggagtatga cccggcgacg ccacaaagga ggtgaaccac
     9421 catgaaagag ctgaaagcca aggcgaaagt gacgcaggcc atgacccgcg acgggctgac
     9481 gctggaaaat caggccaccg gcgaggtgga gaacatttcc agccgggagg ccgaacagga
     9541 tttgtccgcg ccggggccgg gcggcaccgc cgataagctg ctggaacggg ccgacgccat
     9601 ccatgacggc cataaggcca agaaagccgc gaagcaggcc ggggctgtgg ccgccgaggg
     9661 ggacgctgcc ctgcaccggc catccgcccg gctccaactg tcggaggaag aacgggccga
     9721 cccggattta cagccctata tccgaaaatc ggacaaacgg gccgataagc tggataaggc
     9781 ccgcgccgcc atccccaaga agcgcgtccc ggtcaaggaa accgtctttg acgaggcaag
     9841 cggcaaagca aagaccgtcc tgcactttga caaacaggac aaggggcctc cctccctgaa
     9901 acccaatccg gcaggccgtc ctgtttccga ggtttttcta ttcggccacg ggaaaatcca
     9961 tgaagtcgag catgaaaacg tgggtgtgga gggcggccat aagggggagg aacttgccga
    10021 gcggcaggcc ggaaaagcca tccgcagcac catccggcac cacaggctga aaccctactg
    10081 ggacgcggaa aaggccgagc gcaggctatt gtctgccaac gcggagtatt tctaccagaa
    10141 atctttgcgg gataacccgg agatcgcaca ggcggccagc aaccccattt cccggttctg
    10201 gcagaaacag cagatcaagc gccagtacgc caaggccgcc cggacagcgg gccagaccgc
    10261 aggcgcggcg gcacagggcg cggccaagac agccggggcc tccacgaaag cggccaaggc
    10321 cgcagccaaa gaaagtgaac gggcggcctc ctttgtggcc cgccactgga aaggggcgct
    10381 ggccgtcctt gtgttggggc ttttgatgct gcttatcatc ggcggcttac agtcctgcac
    10441 cgccatgttc ggcagcgccg gaagcggcat cacggcgtcc tcttactttt ccgaggatag
    10501 cgatatgttg gccgccgagg acgcctacgc ggagctggaa gccgagctgc aagactatct
    10561 ggacaactac gaggccaccc atgactatga cgaataccat tttgaccttg acgagatcag
    10621 ccatgacccc tatgtgctga tttccattct gtcggcgctc catgacggga tatttaccat
    10681 cggggaggta cagggtgatt tgtccatgct ctttgacaag caatatatcc tgacagaaaa
    10741 cgtcgtggta gaaacccgtt accgcaccga aaccgataca tggacggacg cggagggcaa
    10801 cacccataca gacacctacc aagtgccgta tgactattac atctgctacg tcacgctgga
    10861 aaacttcgac ctgtcccatg tgccagtcta tatcatggac gaggaaacgc taagtttcta
    10921 ttccctgtat atgtccacgc taggaaaccg gcctgatctg ttttccggca gccagtaccc
    10981 caacgcctcc accctgacgc cgccgaccta ctacgacgta cccccggagg ctttggagga
    11041 tgccaccttt gccgccatgc tcaaagaggc ggaaaaatat atcggttatc cctatgtgtg
    11101 gggcggcagc tctcccagca ccagctttga ttgcagcggc ttcatttcgt gggtaatcaa
    11161 tcatagtgga tggaacgtgg gacggctggg ggcgcagggc ctatataaca tctgcacccc
    11221 tgtttccagt tcgcaggcca agcccggcga ccttgtattt ttcaagggaa cctacgacac
    11281 ccccggcgtg tcccacgtcg gcctgtatgt gggcaacaat gtgatgctgc attgcgggaa
    11341 cccgatttcc tacacgaacc ttacttcaaa ctactggcaa tcccattttt acagttacgg
    11401 gcgtttacct taacgccaga aaggagcttt caattatggc aaccaaactt gaccgcatcg
    11461 aaaaggacat ccagaaaacc aagtctaaga tcgcggagtt tcagaaacag cttcgggagc
    11521 tggaaacgca gaaaaccgaa caggagaatt tgcagatcat ccagcttgtg cgcggcatga
    11581 acatgaagcc ggaggaattt gccgcgttct tacgcagcgg cgcaatgcag gccgcccctg
    11641 ccgcaacccc ttaccatgaa caggaggaca atgcccatga agaataagac cagacggatt
    11701 tcccgtattc tccccgcact tctggccgtg atgctctgcg tcacggcctt tcccgttacc
    11761 gcccttgcag gcggcaatga cccggcaccg gccccgctgc cggaggctac aaccgaaccc
    11821 gctacgggcg gtttggagcc tgaaacagac gaaaccggca ccgccacgga cgggacagaa
    11881 accgaggccg aaaaagatac cggccctctg accggcaagg acatcaccga cctgttttcc
    11941 gcgctgttcg gttccaaggt gagcattgcc gccacggacg acggcatcca gatcaccacc
    12001 ggcaccgaca aaaaagaacc tgaacagacc ggcaccgtca ccaccaacgg cggcaggctg
    12061 aatgtccgca ccggcgcggg gcttgacaag accgcgttca cccagcttcc caatgggacg
    12121 acggttgagg tagtcggcac agacggcgac tggattaaaa ttctcttgcc ggagcgcatc
    12181 ggctatgtcc attccgacta tatgaccgtc agcgaaaagg aagtagcggc taccggcgag
    12241 ggcggttttt ccctctccat tgacccggag gaaattgctt ctctgctgga actgttcaat
    12301 ggcggcgggg ccagcgccgc cctgtccccg gacgggaacc tgtcactgat tgacgacatc
    12361 ggcagcgccg cgaaaaatgg caagcagttt atcaccgtgg aaaccaaaaa cggcaatgtg
    12421 ttttatctga ttattgaccg ggacgacgag ggaaaagaaa ctgtccattt cttaaatcag
    12481 gtggatgaag ccgatctgct gacgctgatg gaggacgggg aaaccgtcac ccccgctgct
    12541 gtctgctcct gtaccacgaa atgcaaggcc ggggctgtga acacgaattg cccggtatgt
    12601 aaaaccaacc tgaccgagtg cagcggcccg gagccgcagg aaccccagcc ggaggaaccc
    12661 gaagccccgg aggaagaacc caagggcggc gcgggcggcc tgatcgtgtt cctgctggtg
    12721 gcgctggcgg gcggcggtgc ggccctttac tatttcaagt tcaaaaagcc caaggctgaa
    12781 accaccggca gcgacgattt gggcgagtat gattttgggg aggacgagga tttggaggac
    12841 gaggaacccg aaaccgagtt tgacccggaa gctgattttg tgagtgaaca ggaggacgag
    12901 gaatgacata taaacttgta atcgcggaaa aacccagcgt ggcccgcagc attgcgggcg
    12961 tgatcggggc cacggagaaa cacgacggct atttggaggg gaacggctat ctggtgagct
    13021 ggtgcatcgg acatctggtg tcctttgccg acgccggacg ctatgacgag cgtttcaaaa
    13081 aatggcgcta tgaggatttg cccattctcc cggaaacatg gcagtacatc gtccccgacg
    13141 acaagaaaca gcagtttgac gttctctgtt cgctgatgca gcgccccgac gtgaccggcc
    13201 ttgtgtgcgc caccgacgcc gggcgcgagg gggaattgat tttccgtttc gtctaccaga
    13261 tggcgggatg ccaaaagccc tttgagcgcc tttggatttc ctctatggag gacagtgcta
    13321 ttaaggacgg ctttgcccac ctgaaaccgg gcggcgacta tgatccgctg tatcaatcgg
    13381 ccctgtgccg ggccaaggcc gactggctga tcggcattaa cgccacccgg cttttttccg
    13441 tcctgtacca caagaacctg actgtgggcc gcgtccagac gccgacctta aaaatgctgg
    13501 tggacaggga cgccaagatt accgatttca agaaagagaa atatcacatc gtccatatca
    13561 cggcgggcgg cgcggacgcg gtaagcagcc gcttttccga cgctgccgag gcaaacaccg
    13621 tcaaggcggc ttgtgcaaaa gcgcaggccg tttgcgtttc cgtgacgcgg gagaaaaaga
    13681 cggaacagcc gcccaagctc tatgacctta ccaccttgca gcgggaggct aaccggcttt
    13741 tcggctatac cgccaagcag acccttgact atgcccaggc actgtatcta aagaagctgc
    13801 tgacctatcc gagaacggat agtcaatatc ttaccgacga tatgctgccc gccgtggaaa
    13861 gccttgtgtc cggcctgtgg gagctggtgc cgtttgccaa gggcctgaac ctttccccgc
    13921 agtttgaccg catcctgaac agcaaaaagg tgtccgacca ccacgccatt atccccaccg
    13981 ctgaatttgt gaagcagggc tttggcactc tggccgagag tgaaaaaaag ctgctgtccc
    14041 tgatctgctg caagctctta tgtgccgtgg ccgggccgca tatctatgag gccgtcaccg
    14101 ccacatttac ctgtgccggg caggagttta ccgccaaggg caagaccgtc ctttcccccg
    14161 gctggaaaga gattgaacgc cggttccgtt cttccttaaa gaccgacgcc gacgaggacg
    14221 ccgaggcggt gcgggagctg ccggagcttt gcgagggcca gacctttgca gacgtggccg
    14281 cctctgtcac cgagcatttc accacgccgc ccaagcccta caccgaagat acgcttttgt
    14341 ccgctatgga acgggccggg gccgaggata tgccggagga tgccgagcgc aagggattag
    14401 gcacaccggc cacccgcgcc gccattctgg aaaagctggt gcagatgggc tttgtccaga
    14461 gaaaaggcaa gcagctcatt cccaccaagg acggcatcaa tctggctgtg gtgctgccgg
    14521 aggcgcttac ttctccccag cttactgccg aatgggaaaa ccggctgacc gagatcgcca
    14581 aggggcaggc cgatcctgac gaatttatgg acggcatcga gacgcaggcg cgggagctgg
    14641 tgcagaccta ttcctgtatc agtgaggaaa agcagaaatt gtttcaggcc gagcgtgttc
    14701 ctatcgggac gtgtccccgc tgtggtgagg ctgtctatga gggcaagaaa aactactact
    14761 gcggaaaccg ggcctgccag ttcgtcatgt ggaagaatga ccgatttttt gaggaacgca
    14821 agaaagcgtt caccccgaag atcgccgctg cgctgctcaa aaccggcaaa gtcaaggtaa
    14881 agggcctcta ttccgtaaag accggcaaaa cctacgacgg gacggtgctt ttggctgata
    14941 ccgggggcaa gtacgtcaac taccgcattg aacacaggag ctaaaggaca gtaacacagc
    15001 aagcataggg agtgtggcta cactcccggt aaaatccccc ggccgcgtcc ctgccgggcc
    15061 ggggattttt gcttgttggg gaagtcccca accccctatt ttttgaaaca gaacagagaa
    15121 aggagtgacc gcgtgaacga agtattttct atccaagtcc ataaccggca gtttttggaa
    15181 cagggcaaac aaggcgtctg gctggatttg cccaccacaa ccgaaacgct gcaagccgcc
    15241 ctgcgggatg ttggcattac tgccgacaat ccgcaggatt tttttatcaa cgggtattcc
    15301 tgcccggagg acagacacct tgccattccc tatgacatgg tgctggccgc cgacgtggac
    15361 gagctgaatt tccttgccgc ccggctgggc cagcttgacg ccgccgagat cgcagagctg
    15421 aacgccgccc tgcaaaatcc ccgtggcggc tttgagaata tcggccagat catcgactac
    15481 accgaaaatg tggattacta tgtgcatctt cccgacgtta ggggggatgg acagttgggc
    15541 gactactacc tgaaccgttc cggcatggtg gatatgccgg aggaatggaa agccgggatt
    15601 gatacggcgc agtttggacg ccacattgcc cagcaggaac aaggcgtttt tacccagtac
    15661 ggctatctgg tgagaagcgg cgacgaatgg cagcgcgtcc atgagggcca gccggtgccg
    15721 gaggaatacc gggtgatgca ctacccccag ccggaagctg accgggagga accgcaggcc
    15781 cagcagccgg aggcatccga aagccccgac aaagcgccgc ccccggttat cccgatcatg
    15841 ctggacggca tgaacagcaa agaacgcatg aaagtgatta ctgaccgtct ggaaaccggc
    15901 gtacaggagc tttttgaaag tagccgctat atggcttact tatccgctat gtccaagatg
    15961 cacgattaca gtctgaacaa tacgctgctg atcgccatgc agggcggttc gattgtggcg
    16021 ggctataaca ggtggcagga tgaatttgac cgccatgtga agcggggcga gaaaggcatt
    16081 aagatactgg ccccggcccc gttcaaggtt aagcagcaga tggagaaaac cgacccggcc
    16141 acccagcagc ccgtgatcgg cgcggacggc aagcccgtga ccgaagaaca ggaagtgacg
    16201 gttccggcct ttaaggtggt gacagtcttt gacatcagcc agaccgaggg caaggatttt
    16261 cccatgccct atatcagtga acttaccggc gacgtgcagc agtatcagga tttctttgcc
    16321 gcgctggaac ggacatcacc ctatcccata agcattgagc cgatttcaga cggggccaag
    16381 ggccgctgct tctacgcaaa acagcgcatt gccatcaacg agggcatgag cgaattgcag
    16441 accctaaaga ccgccatcca tgagattgcc catgccaagc tccacgccat tgaccgggag
    16501 gcccccgccg aggaacaggc ggcccggcca gaccgcagca cccgcgaggt acaggccgaa
    16561 agcgtggcct ataccgtctg ccagtattac gggctggaca cgtcggacta ttcttttggc
    16621 tatgtggccg attggagcag cggcagggag ttatccgagc tgaaagcctc ccttgaaacc
    16681 atccgggcca cggccagcga attgataacc gagatcgacg gccattttgc ccagcttcag
    16741 caggagcgcg aggcccggca ggaacaggag gccgtcacgg ttgacgttac cccggaggca
    16801 gcggccccgg cgcaggccga ggaagaacag gccccggccc ttgatccggc agccgagccg
    16861 cttgtgactg tgctatggag cgaaagcccc cagctaaagg acggccagca aatgccgttg
    16921 catgaggccg acgcgctgtt tgcccgtctg gatgcagcat atccggcaga ggcaggctat
    16981 gacaaaaccg cgttccgcat tgactgtatc tttcagggcg agccggacag ctacgagggg
    17041 cggcaggatt ttggagatag ggacggttcc ctgatcgaac atatccagag ctaccaccaa
    17101 ttttacgcgc aggacgaaaa ctggaaaaag catgtaatcg ctacaaccgg catagagggc
    17161 tgggagcagg acaaggccca tcgggatatg ctgttaggcg agtttgtccc ctatatgaag
    17221 ctgcattgca gccttgccca aatggaacag acggcgcagg gccgcttaca gtccggggaa
    17281 accctgacgc cggaggaaac ggcctatttg ggggcgcttc tctcttatgt gcaggagtgc
    17341 cgccccatgc tcaatcaggg ccagtatcag ttgccggagc cgccgaaact ggctgacttc
    17401 gaccagagct tgcaggacta caaagcacag gtacaggccg agatcgcgca ggaggccgcc
    17461 gcagccggta tgaccgtgga ggaatacgcg gcggctggct atgaagcacc ccaacaggac
    17521 accttttcca tctaccagct tcgaggcggg gaggaaaccc gcgactatcg gtttgagcca
    17581 tacgagcgtt tacaggccgc agggctggcc gtggacagtg ccaactatga ccttgtttat
    17641 acggcaccgc tggataaaag cacgtctttg gaggatattt accgcacatt caacatcgac
    17701 cacccggcag attttagggg acacagcctt tccgtttccg acgtggtggt gctgcaccag
    17761 aatggacagg acaccgccca ctatgtagac agcttcggct atatggaagt gccggagttt
    17821 ttgcgggaaa atcccctgcg taccgccgaa ctgtctacgg agcagaacga aaacatgatt
    17881 gacggcatta tcaacaatac gccgcccgaa ccgcagccgg aggaacccga acagctatct
    17941 tttgaggatg tggcagttgc ttccggccag acacaggagg aacccccgga ggctgcgccc
    18001 ccgcaaacgg ctgtccgcta ttatcccatc aatgagggag ccgcccgccg cgccaaggaa
    18061 gccatgagct tttctgacta ccggcccggc agcgccacgg cggaataccg gcactatgtc
    18121 gatcaggccg tggaaattgc ggagcgccag aaaaagcgtg tggagccaga gtatcacgaa
    18181 aagatagaca gcctccttga cacctacgcc cgcaagctgg ccgagaacat gaataagggc
    18241 tatgagatta ccgcccgcgt cccctctgtc ctgattgccg ggccgtccaa tttcccggta
    18301 cgggcgaaag aaaagcagaa cgccgccagt gaccgcaaca tggaggaatt tcagtacatt
    18361 cagggcttgc ttgacaaaat ccgcagcacc ggcatgggtg gtatcagcgc cgacgatccg
    18421 caggccgttt ccaagctgga aaagaaactg gaaaagctgg aagcgtcgca ggagttgatg
    18481 aaggccgtca atgcctacta ccgcaagcat gggacgctgg acggatgccc ccatctgatc
    18541 gaaagaggca ttgaaaacct gaaagcggat atggcgtcgg gctggcacta tgagaaaaaa
    18601 ccgttccagt cctggcagct ctccaacaat aacgccgaga tacgccggtt aaagggccgc
    18661 attgaggaat tgacccgcca aaaggaggcg gcctatgtgg gatgggagtt tgacggcggc
    18721 actgtggaga tcaaccgggt ggccaaccgc ctgcaaatct tctttgaggg gaagccggat
    18781 gctgctgtac gcgacgagct gaaaagcaac ggtttccgct ggtcgcccaa ggccggggca
    18841 tggcagcggc agttaaacga caccacaatc cgcgtcgctg accgtatcaa gtgcatccag
    18901 cccctttccg gcgaaaagcc cagcgcctta cagcttgcgg cccgcaggga gaaagaaaag
    18961 ccctctatcc gggcgcagct caacgcggcc aagaccgaac aggccaagca gccaccggcg
    19021 cgggaaaaat caaagggatt ggaggtagag taaatgcaga agtttgaaaa tgtggacgtt
    19081 ctggccgcgc tggaacagat catgcggcag aacaccgcgt tttatcaaag tgactttggc
    19141 attgacaaag acattatcag gcgggatgcc gccagcgatc aggccgcaga caagaccctg
    19201 ctctggatgt cccggccctc cgggacgtac tgtttccggg aacgtgatgt tttcctgaaa
    19261 gacacccgac agtacaacac atggaaattt tacggggagc agacccgtga caagatactg
    19321 gcctacgcgg tggagctgac cggcacacag ggcggcacta tccggggcaa tctctatgag
    19381 ctggactatc cgcagcattt ccggcacgtc attgaaaccg cccagccggt gagtgtgaac
    19441 cggctctttt atgagcatgg cacccgtgac atcccggaga gccagcgttt tgacggaagc
    19501 cctgaccggg tattgggaaa ctttttgtac tatgaggccc agccccatga cccggctgtg
    19561 ctgcaggagg cgctacggac ggagcagcac agccgggggc gggccgtccc cggcgatttc
    19621 aaagtccacg tcaccgcttt gcatgacagc cggattgaaa ccgaggcccg gcgtattgtg
    19681 gacagcctga aagatctgtc cgcgcctaac agccccaata aaacgcattt catggtggag
    19741 atctccccgc actttgcggc gattgcttcc accaaggata atgaccggct ctttgccatg
    19801 ctgccctata aatccctgtg ttttaccggg atgaaaggca ggcatggcgt gtatgccgtg
    19861 attaaccggg acgagcgccg ggatgtgagt attcgcaggc cccggccctc tatccgcaag
    19921 cagctatccg acagcaaaca ggccgccagc ccaaaaaagg cggcggccag aaccaaaaag
    19981 aatgaattgg aggtataagg aatgatgcac tttaccgtgg aggaagaaaa cctgatctgt
    20041 atgtaccaca atgcagaccg gcgcagaacg ataaacaggc tcaccgccgt catgccggtt
    20101 atggatgggg atatgcgggc gcaggcccag cagacccttt ccaagctgga acgcatgacg
    20161 gatgccgact atgacggcca gaagttccac ttcacagacg aataacgaac agcagacgcc
    20221 gggcgcggcg gccatgtggt cgcttgcgtc cggcgtcttt tctttttgcc tattcctacg
    20281 catttagaac ccggtattgt cggggatagg atgaagtatt cgcttgcccc tgcaaacatg
    20341 cgggagaagc cttgcagcgg gcggttttca atcccttaaa tgcgtaggtt gcagctataa
    20401 ttagacccct gaaacagata tagccgcgca gcgggtgtat ctgtttcagg ggtgtgcagg
    20461 tgatagccgc aaagcggcgt aagagggtgc agccctcttg ccgtctatct ctgttccagc
    20521 gggaagctcg gcaatgcttc cagcagtttc agggaaatgc gctgccgtat atcttcgtca
    20581 atgactttct tctttttgcc gtttgcctgt ctgatctcca ccgtggaaag ctcgttgatg
    20641 tagtcagcat aatgggccaa cactttttcc gtggcccacc tctcaccggc aacggcggct
    20701 ttgatcgttt catagtccag cagcttttct tgtccgtcca tcctgatacc cccgtcagac
    20761 tgattttttc tatgatacca tattcggaag catggaacca gagaaaacgg cgcttttctg
    20821 ctggtaaatt ctacgcattt agaacagcac ttcggcgggg ataggataaa acacccacat
    20881 accccgctga ccatgccgga aaagccttga aatataaggg cttttacagc tctaagtgcg
    20941 taggtttcca gtatcttttc cgctggcgct cggcctcttt gcggcggcgc atccgtttgg
    21001 agcaggccgc gcagtatttg gcccggttag acttcggcgt gaacgtggcc ccgcactcac
    21061 agcagcgttt cacttcgtcc cggtctttgg tgatctcggc atacagggcc gcatccagcg
    21121 ggaggacagc ggccttaaac cagcggcaca gaagcgaata ggaaatgctc tgtacgcaaa
    21181 tacattcttc gccgtcgtcc agcaacaggc agttgccgtc acaatagttg cagcactcat
    21241 gcgtcagctt ccgggccttg cgatattgag ggtaattcat tcgtggaata ctcatagctc
    21301 ttgcgtccat tcctttcccg gcgtcatacg caaaatgccg tccacattct gcttgatgat
    21361 gccgtattcc tgcatctgct ttttcgcttc gccgtacttc tcatacagcc gttccttttc
    21421 ctcggccaat cgcttgtact cggctttcag ggcgtacaga tcggggagct ttttcacacc
    21481 ctgtcccttt agggcctttg ccgcagcttc atacaaaatc aacgtgcttt catgctcacg
    21541 ccggaatttt gccttatccg ctgcattgcg gtattccata gccacgggcc agagctgctt
    21601 gtaggtagaa atattcttta tcagcaccgc catatccgca agccggtgtt ccatagcttt
    21661 gagggtagcg gcggcctcgt cgctggcggc ggtgatctcg gccactttgc tttccaaatc
    21721 cgggtaggtg tctatcccat gttcagtcag gaaattcaaa gtctttgcgg cctgcttcaa
    21781 attgtggagc ttcgcccacc tctcatagcc tgccgactgc tgggccttaa tgttgttttc
    21841 cagatcaatc cgcaaagaaa tccccttgcg ctctttgggc gctctggtga taacgcggcc
    21901 tttgatacgc tctttgatgg cttcctcggt gtaggcttcc cccaatgtct tgcagcgggt
    21961 aaagcgttcc tgtccgggag cgcggaaaga aataaacttt cccggcttga tctcatagcc
    22021 ctgcgcctgc atgagccgca ggaaatcatc aaaatctttt gcctgcggga tagcggcgtc
    22081 aatggctgct ttgagcttcg ctttccagct cgtccctttc cgctgggcgt cccactcggc
    22141 atagctcttg cccttactct ggccgggctt caccacggac aatccgtttt ccttgcacag
    22201 cctgtcgctg gtgcgccgga tataggcata gctctgtttg ttggaaatgt acttgcgctg
    22261 atttaccata tccacggcac agaaaataat gtgattgtgg acgtggccct tatcaatatg
    22321 ggtagtcagc acatagggat atttcccttg cagcacttcc tcggcaagct gcttcccgat
    22381 ctcatgggcc tgttccggcg tggcttcgcc gggggcaaag gattgtatca ggtggtggcc
    22441 cagattattg cccttttggt atgcctgcgc cagcagcatt tcaaattcaa tgtccgctgt
    22501 ttcataagag caggcaaagg aggccaccag cagcttgccg tcagttttgt ccgggttctc
    22561 gatgtagtca agggcttttt tcagcgttga cttgatagga tgtatctttg taactgccat
    22621 atctgcgcca gcgccccctt tatatcctcg atgtcctgtg cgtacaccgg ccccataccg
    22681 ttcagcctct ttgcaatctg attgacgttg acgccgatct tctgtatctc ggcggtctgc
    22741 gcccggatgt cgctggtgtc catgttgacg atatagccgt caatgagcat cttccgggcg
    22801 taggctgaaa agttcttcgt atgcagcagc gccattttct gttcaatcat agcccgttcc
    22861 tccggcgtca cagggacgcg cagcacgatt tttcgctttc tgtttgccat gcttcaccgt
    22921 cctttccatt caggggtttg gggatttccc caacaagcaa aacggacgcg gcccggcagg
    22981 gacggggcag gccgtttttc cgggagtggc tacactccct atgcttgcta cgttactgtt
    23041 tctccactaa caagacgttt ccaaagcccc aaactacccg caaaatagcc ctctggaaag
    23101 aaaaaagtgc aaaaaaatag aagctacgct gtgcagctcc cgctaaaagt aaaacagagg
    23161 atgccagtta cacatcctcc gtttcattct tcttcggctt ttcaattagt atggcgctgt
    23221 caatcgcgcc ctcgatgatc ggcaggtatt cttcggggca gagtttcagc ttgtggctga
    23281 ccctttgccg cagttcgctt tcctcccgca tgatctccgg gttaaagtag cgttccacag
    23341 gaagcccgca ggctttaata agctgtatga caaccggcag actgggaatg gcccgtcgtt
    23401 ttcgatgttg gcaagatagc gccagtcaat atttattttc tctgccattg ctttgcgcga
    23461 aacgcgcttt gcttgccggg ccgctttcac atccgcgccg aaagtttcaa agccgggaca
    23521 atcttcaata ttcgccatat cgcacctccc acttacattg tatatttcat actctgcccg
    23581 tggaatgttg gtatatgcac ctttcttaaa gtttatattt cacattttgt ttcttttatt
    23641 cttcgagcaa aagaattata atgtaccctg tggaggtgta ctatggacta catgacgttg
    23701 aaagaggcag gcgagaaatg gggtgtgccc ccacgatgga taaactacta ttgtgctgcg
    23761 ggacgcatcc ccggcgctgt aaagatggcg acgatctggc tcattcccaa aactgcggaa
    23821 aagccgattg acaggcgtac aaagaaagga aaggcggtta ctgaatagta taatgaaacc
    23881 ggcgcatttt ggatatggtt ttgaagagag agaaagcatg gtattttgtc acggaaaaac
    23941 tataagatga tagatggcag gctgctccag accgataaaa agtattctca tctgaaacag
    24001 aaagaaaaga tttacacatg gatgtatgaa gaagcgaaac tttatcatga cagaaatgga
    24061 aaatgcccga aacaaagacg aggaagctgc tgctgtatca gccgtatatg gtagaattga
    24121 aaaggccggc atctggattc tttatggtga agtctccaga cattggataa ctgctggtct
    24181 aatgcgcctt tgagtatggg gtgattgatt ttaataccgg ggaggtacgg ctttttgagg
    24241 aaaatattct gggatttaca tttggcgatt attacatttc ctgtaactgc aaggacacgg
    24301 atgatgaaga tatgaggaat atagacttgc tgattttcta ctgtcttgaa cggtattagg
    24361 actgaatctg ctgcgcggag tgcggcaaga gcgaccggag ggagtacaca caggccctgc
    24421 ctgtgtgaaa agccataccg caaggtgcat tcgcaaaaaa gaggccgcgc attttgctga
    24481 ggcttggggt gtccccaaca agcaaaacga gaacgccgca ggcgggcaag tggtggatca
    24541 gaatatccgt aaaggatatt ctgatccatt gcttgccagt attatcggga tgttttgaca
    24601 accgctgtaa tcgaaacatc ccaaacatta agaaggtgtt aattattatt atcttgattt
    24661 aattcgttta aaccaataat atagatacat acaccatgaa aagaagcgag tatcccaatg
    24721 acggatgatt aaaatccgaa aagaaatcgc tttgaaacta cagggaaact acagactgtt
    24781 atgttatctt cttaaaatgg agggattttt atgtcgatac gaattctact tgtcgaggat
    24841 gatgatcata tctgtaatac agtaagggcg tttctggctg aggcaggata tcaggtggat
    24901 gcctgcacag atggaaatga ggcatacacc aagttttacg aaaacactta tcaactggtt
    24961 attcttgata ttatgctgcc cggtatgaac gggcatgaac ttttgcgtga atttcgtgcg
    25021 aaaaatgata ctcccattct gatgatgaca gccctgtcgg atgacgaaaa ccaaatccgg
    25081 gcgtttgatg cagaggcaga cgactatgta acaaagccat tcaagatgca gattttacta
    25141 aagcgggtgg aagccctgct gcggcgcagc ggtgcgctgg caaaggaaat ccgtgtcggc
    25201 aggctgacac ttctgccgga ggatttcacg gtactttgtg acggtacaga gctgcccctg
    25261 acacgaaaag aatttgaaat ccttttgctg ctggtgcaga acaaaggcag aaccttaacg
    25321 catgaaatca ttttgtcacg tatatgggga tatgactttg agggtgatgg cagcacagtc
    25381 cacactcata tcaaaaattt gcgggccaag ctgccggaaa atatcatcaa aaccattcgc
    25441 ggtgtaggat accgattgga ggaatcataa taatggaaag aaaagggatt ttcattaagg
    25501 ttttttccta tacgatcatt gtcctgctac tgcttgtcgg tgtaacagca acactgtttg
    25561 cacagcaatt tgtgtcttat ttcagagtga tggaattaca gcaaacagta aaatcctatc
    25621 agccattggt ggaactgatt cagaatagcg ataggcttga tattcaagag gtggcagggc
    25681 tgtttcacta caataaccaa tcctttgagt tttatattga agataaagag ggaagcgtac
    25741 tctatgccac accaaatgcc aatacatcaa atagttttag acccaacttt ctttatgtgg
    25801 tacatagaga tgataatatt tcgattgttg ctcaaagcaa ggcaggtgtg ggattgcttt
    25861 atcaagggct gacaattcgg ggaattgtta tgattgcgat aatggttgta ttcagccttt
    25921 tatgcgcgta tatctttgcg cggcaaatga caacgccgat caaagcctta gcggacagtg
    25981 cgaataaaat ggcaaacctg aaagatgtac cgccgccgct ggagcgaaag gatgagcttg
    26041 gcgcactggc tcatgatatg cattccatgt atgtcaggct gaaagaaacc atcgcaaggc
    26101 tggaggatga aatcgcaagg gaacatgagt tggaggaaac acagcgatat ttctttgcgg
    26161 cagcttctca tgagctaaaa acgcccatcg cggctacaag cgttctgttg gagggaatgc
    26221 ttgaaaatat cggtgactac aaagatcatt ctaagtatct gcgcgaatgc atcaaaatga
    26281 tggataggca gggcaaaatc atttccgaaa tactggagct tgtcagcctg aatgatggga
    26341 gaatcgtacc catagctgaa ccgttggaca tagggcgcac ggttgccgag ttgctgcccg
    26401 attttcaaac cttggcagag gcaaacaacc agcggttcgt cacagatatt ccagccgggc
    26461 aaattgtcct gtccgatccg aggctgctcc aaaaggcact atccaatgtc atattgaatg
    26521 cagttcagaa cacgccgcag ggaggcgagg tacggatatg gagtgagcct ggtgctgaaa
    26581 aatgccgcct ttttgttttg aacatgggcg ttcacattga tgatactgcg cttccaaggc
    26641 tgttcacccc attctatcgc attgatcagg cgcgaagcag aaaaagtggg cgaagcggtt
    26701 taggacttgc catcgtacaa aaaacgctgg atgccatgag cctccagtat gcgctggaaa
    26761 acacctcgga tggcgttttg ttctggctgg atttaccgct cacatcaaca ttgtaaatat
    26821 ttaaaactta aatgattt
//
LOCUS       DACCXO010000029        26102 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.030, whole genome shotgun sequence.
ACCESSION   DACCXO010000029 DACCXO010000000
VERSION     DACCXO010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.030"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(1..1032)
                     /gene="queA"
                     /locus_tag="IVY70_001138"
     CDS             complement(1..1032)
                     /gene="queA"
                     /locus_tag="IVY70_001138"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP7958278.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1225..2402
                     /locus_tag="IVY70_001139"
                     /pseudo
     CDS             1225..2402
                     /locus_tag="IVY70_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly.; frameshifted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
     gene            2406..3047
                     /locus_tag="IVY70_001140"
     CDS             2406..3047
                     /locus_tag="IVY70_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7958279.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3044..3961
                     /locus_tag="IVY70_001141"
     CDS             3044..3961
                     /locus_tag="IVY70_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7958280.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            3958..4629
                     /locus_tag="IVY70_001142"
     CDS             3958..4629
                     /locus_tag="IVY70_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7958281.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5170..5658)
                     /locus_tag="IVY70_001143"
     CDS             complement(5170..5658)
                     /locus_tag="IVY70_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP7958282.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(5859..6125)
                     /locus_tag="IVY70_001144"
     CDS             complement(5859..6125)
                     /locus_tag="IVY70_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP7958283.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6125..6388)
                     /locus_tag="IVY70_001145"
     CDS             complement(6125..6388)
                     /locus_tag="IVY70_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7958284.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(6381..6653)
                     /locus_tag="IVY70_001146"
     CDS             complement(6381..6653)
                     /locus_tag="IVY70_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958285.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            6864..7049
                     /locus_tag="IVY70_001147"
     CDS             6864..7049
                     /locus_tag="IVY70_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7958286.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(7191..9014)
                     /locus_tag="IVY70_001148"
     CDS             complement(7191..9014)
                     /locus_tag="IVY70_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP7958287.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9612..12317)
                     /locus_tag="IVY70_001149"
     CDS             complement(9612..12317)
                     /locus_tag="IVY70_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP7958288.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(12623..13570)
                     /locus_tag="IVY70_001150"
     CDS             complement(12623..13570)
                     /locus_tag="IVY70_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP7958289.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            13760..14029
                     /gene="rpsN"
                     /locus_tag="IVY70_001151"
     CDS             13760..14029
                     /gene="rpsN"
                     /locus_tag="IVY70_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP7958290.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(14205..14567)
                     /locus_tag="IVY70_001152"
     CDS             complement(14205..14567)
                     /locus_tag="IVY70_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7958291.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(14578..15699)
                     /locus_tag="IVY70_001153"
     CDS             complement(14578..15699)
                     /locus_tag="IVY70_001153"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP7958292.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(15715..16065)
                     /locus_tag="IVY70_001154"
     CDS             complement(15715..16065)
                     /locus_tag="IVY70_001154"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP7958293.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(16195..17706)
                     /locus_tag="IVY70_001155"
     CDS             complement(16195..17706)
                     /locus_tag="IVY70_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP7958294.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(18028..19380)
                     /locus_tag="IVY70_001156"
     CDS             complement(18028..19380)
                     /locus_tag="IVY70_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP7958295.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(19533..20609)
                     /locus_tag="IVY70_001157"
     CDS             complement(19533..20609)
                     /locus_tag="IVY70_001157"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP7958296.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            20805..22550
                     /locus_tag="IVY70_001158"
     CDS             20805..22550
                     /locus_tag="IVY70_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP7958297.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(22830..23267)
                     /locus_tag="IVY70_001159"
     CDS             complement(22830..23267)
                     /locus_tag="IVY70_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP7958298.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(23251..24624)
                     /locus_tag="IVY70_001160"
     CDS             complement(23251..24624)
                     /locus_tag="IVY70_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958299.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(24946..25434)
                     /locus_tag="IVY70_001161"
     CDS             complement(24946..25434)
                     /locus_tag="IVY70_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7958300.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(25456..26058)
                     /locus_tag="IVY70_001162"
     CDS             complement(25456..26058)
                     /locus_tag="IVY70_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP7958301.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         7491 a         5550 c         4829 g         8232 t
ORIGIN      
        1 ttatttcacg aacatcgcat ctccaaaact aaagaaacga tagcgctctt caatcgcatg
       61 ctggtaagca gataaagtca attctcttcc tgcaaaggca ctaactaaca tgactaaagt
      121 cgattttggc aaatggaaat tagttgagaa agcttctacc actttgaatt catagccagg
      181 cgtaatgaaa atatcagtcc atccgctgtc tgctttgatt tctccattaa atttcgttcc
      241 aatcgtttct aatgttcgaa ttgaagttgt tccgacagcc acgattttcc cgccattttt
      301 acgtacttgg ttcaatcgct cagccgcttc ttccgtcaaa cggtagaatt cactatgcat
      361 atggtgttct tcaattttat ctacgctgac aggtcggaat gtacctaacc ctacatgtag
      421 tgtcagatag actaattcaa caccctttgc ttcgatctca tcaagtaatt ctttcgtaaa
      481 atgcaatcca gctgttggcg ctgcagcaga accattttct ttcgcataga cagtttgata
      541 gcgatctggg tcttccagac gttctttgat atatggaggc aatggcattt ctcccaatga
      601 ttcaagaatc tctagaaaaa ttccttcata ctcaaagtca atgatccgtc cgccgtgttc
      661 caattcttct ttcactactg cttgcaatct gccatcacca aaagagattc ttgtcccaac
      721 tttggcacgt ttcgcaggtt tgatcaatgt ctcccacgta tctccttgtg tattcgttag
      781 aagaaggact tctaaatgcg caccagtatc tggtttttct ccatataaac gagcaggcag
      841 aactcgtgta ttattcataa ctagcgcatc accaggctgt aactcttcaa tgatttcatg
      901 gaaatgttta tcttccatct ctccagtttt ccgatccaaa acaagtaaac gagaatgatc
      961 gcggtctttt aatggtgttt gtgcgattaa ttcttccggc aagtcaaaat caaaatcttc
     1021 tgttgttaac atatcgtcac tcactttcta tcatgtgctt catcattcta tcatgttttt
     1081 cgttctgttg gcaaaatcag cactttttca cttctatttt ttgttttcct aaataaaaaa
     1141 actaccctat ttgtattttt tcattgtttt aggcatacta gtaatagata gttcttcatt
     1201 gaaaatagaa aaagaggtgg caccatgatt gaatttcaaa atgtgtcaaa aatttataaa
     1261 ggtggaaaag tcgctgtaga ggacgtcaat ctttcatttg aaaagggtga gttcatttgt
     1321 tttatcggta cgagcggaag cggaaaaacg acgacgatgc ggatgatcaa ccgaatgact
     1381 gatccaacac aagggaaaat tttgattgat ggaaaatcca tccaagaaat tgatccagtg
     1441 gaattgcgac gtcagatcgg ctatgttatc caaagtattg gattgatgcc tcacatgacg
     1501 atccgtgaaa atatcactct tgtccaaaaa ttgttgaaag tcagtcaaga agaacggaat
     1561 aaaacagctg aaaaaatgat tgatcttgtg gaattgcccc gtgagatgtt agatcgctac
     1621 ccacatgaaa attatctggt ggtcagcagc agcgtattgg agtcgttcgt gcccttgcag
     1681 cgaatcaaga tatcatcttg atggacgagc cattcggtgc gcttgatcca atcactcgtg
     1741 attccttgca agaccttgtc aaagatttac aagagcgttt gggaaaaacg atcgtatttg
     1801 taacccatga catggatgaa gcgattaaac tagcaagccg tatcgccatc atgagtgaag
     1861 gccgtgtgat ccaatttgat acaccagaaa atattttacg tcatcctgca aacgatttcg
     1921 tagaagagtt gattggagaa gatcgcctac tacaagccaa gccaaatgcg acaagagtcg
     1981 gcgaagttat gctaaataca gcaatcacga ttacaccgga aaaatcatta caagaagcaa
     2041 tcaaactgat gagggaaaaa cgtgtagata cgttgttagt taccgataat tcgaatgtat
     2101 taaaaggatt tatcgatgta gaaacgttga ataaaaaacg aggaaaagta tccagtgtcg
     2161 gcgacatttt gaataaacat gttttttacg taaaagaatc cgcctatctt cgtgatacct
     2221 tgcaacgcat tttaaaacgc gggctgaaat acgtgccggt cgttgatgaa cagaacaaag
     2281 tcgtaggtat cctgactcgt gcatcattag tggatatcgt ctacgatgtg atttggggag
     2341 aagatcccgc aactactgat gttgcagagt cagctactcc tgaagaatct aaggaggtct
     2401 gacctatgca gcagtttttt catcagcacg gttcagaaat tctctcaaaa ggttttgaac
     2461 atctttatat ttcaggactt gctttgatca ttggtatcat cattgctgta ccgcttggtg
     2521 tcttactgac acgttttcca aaagctgcca cgattgtgat tggcttaacc agtgccttgc
     2581 agacaatccc ttctcttgct ttattggcat tgatgatccc gctatttggc gtaggtaaat
     2641 tacctgcgat tatcgcactc tttatttatt cattattgcc gattttacgt aatacgtaca
     2701 ttgggatgaa aaatgtcgat tggaattatc gggatgtggc aaaaggcatg gggatgactg
     2761 agttccaatc cattttttcc gttgaattgc ccattgctat gccaacgatc atggctggta
     2821 ttcgtttagc tgccgtttac gtgattgcat gggctacact tgcctcttat atcggcgctg
     2881 gtggtttagg cgatttgatt tttagtggac tgaataatta ccaaccagat ctcattttgg
     2941 caggtacgat ccctgtaaca attttagcac tattggctga ttggcttcta ggtctcttgg
     3001 aacaccggct gacaccgatc gcacttagag aggagaatga agaatgaaac gtatactaaa
     3061 aatgaccatg gttcttttct ctcttcttat acttgcgagt tgttccttcc ctggtcttgc
     3121 cagcaataca gatgaagaca ccatttcgat cacaggtgga atcacttcag aagcacagat
     3181 cttagcaagt ctagttgccg gaatggttga acattataca gataaaaata ccgcaatcat
     3241 caacaatctg gctactacaa ctatcaacca tcaagcgatg atgaacggag atgcttcaat
     3301 ttcagcggct cgctacacag gaacagattt aacgactaca ttgaaccttc ctcctgaaaa
     3361 agatccgaaa aaagcgtttg ctaccgtgaa agatgaattt gagaaaagat acggtcaaac
     3421 gtggttcccg tcctatagtt ttgaaaatac gtatgtcttt cttgttcgaa aagacaccgc
     3481 acaaaaatac catctatcta aagtcagcga tctgaaaaat gtagcggatg aattagtagc
     3541 tggtgtagat acttcttgga tcaatcgtaa aggagacggt tatgacggct tccaagaaac
     3601 ttatggcttc tccttcaatt cgatattacc tatgcagatt ggacttgttt atgacgcagt
     3661 cgaagcagga aaaatggata ttgttcttgg ctactctacg gatggacgga tcgccagtta
     3721 tgatctagtc atgttggaag atgatcgtca attcttccca ccatatgatg ctgctccagt
     3781 tgtagacaac aagatattag ctgatacgcc tcatttaaaa gaagccttaa ataagttagg
     3841 tcataccatc agtacggaaa aaatgcagga attgaactac gaagcagata ataatctagt
     3901 ggaaccatca gtagtagcac aacgtttctt acaagaaaac cactattttg aagggaagtg
     3961 aagcaattgg ataaaatgaa tgtctttcaa caatttttct attatttcca ggaaaatggc
     4021 tcctatatct ttgcacaatt tatccgtcac ttcctgattt ctatctatgg tgtcctcttt
     4081 gcagcagttg tcgggattcc ggttgggatc atgatttcta ggagacgtaa attagcaaac
     4141 tgggtgattc gtttggcgaa tatcatccag acgatccctt ctctagccat gatctccatt
     4201 ttaatcattg gattaggcct gggagtcaat gtcgtgattg ttactgtatt tttgtattcc
     4261 ctcttaccga tcatcaagaa tacgtatacc gggatgatcc aagtagacaa aaacatcctt
     4321 gatgtcggca aaggcatggg gatgactgct agacaacgac tattcatggt agagttacca
     4381 ctctctgttt ctgttatcat ggcaggcata agaaacgcgt tagttgttgc catcgggata
     4441 accgcgatcg gtgcattcgt cggtgctggt ggtcttggag atatcattat ccgaggaacc
     4501 aatgcaacag agggcacatc gattatttta gcaggtgcat tgccaactgc attaatggcg
     4561 atcatcacgg actggctttt aggtattttg gaaagacggc tagatccagc cagccgtaca
     4621 tcacgctgat gttattgtaa aaaacaaaag cttctgcgac catttgttgg tcgcaaaggc
     4681 ttttttagtt ctgtcatttt taaggcaata atccactctg attcttccct ccttatgggc
     4741 aaagagttgc ttttcttagc tatttatcca ttccgtatac tccaacttca tactgccaac
     4801 gggttcatat ctgcatccac cgactaagtt tttttcttat ccgctaaggc attttgccaa
     4861 tccattttcc catcactcgt catggaattc ataccacatg atctctaaat actcgtctgc
     4921 ttgttctttg aattgggcat caattcttac atcctttggc gtggctttca tttaagcctt
     4981 ttccatcagt cctcgattgt tcgatgcctt cagctgcatc atcaaaactg gtgactgccg
     5041 tatcggtaac ttcgatcatc ttttatacaa tcgggaactt gccatcctca tccttggcta
     5101 aaaacatatc tagccattcc agcttctcgc catgggaggt ttgcggttcc taaaaccggt
     5161 acacgattct tatggactgc atccgtcgca actagacttg gcgatacgat gatccttcac
     5221 cagaagagca cccccgtaaa ccaattgatc gatatattgc caataagaaa agacattggc
     5281 gtaaaagata tcgatccctc gtgatgcatt gccgttgata ctgctattca tggccgaaag
     5341 cgccacgact ttaacttctt ccgtttgagt gacattcatg ttcggcaatg tcgctttgga
     5401 tacccgctct gctaaaggaa cgacactggt attgtatttc gtatctgtat cttttccaaa
     5461 agaccatgcc aacgaatcct ctgggaacca ataagacgac tccggttgat tgtccattgc
     5521 ttttttcaac atacttttgg ctgtttctcg ataaacgaca tttccaattg tcttcgcctc
     5581 actttggcga taatcgatgg tcgttgccac tgccaacact aacaacgatg ctccaatttt
     5641 actgattttc cttgtcacac ttcttcctcc ttgttttaca taaacccgtc aacattccat
     5701 tcctaaactc aaacaatgac ccttcaaagg tggagatata ggaacaaatt aaagaaatca
     5761 tgcaataaat ccaagaaggt tggttttaga acaaaaaaag agtctccttt ttggggagac
     5821 tctttgttaa ctatctaaaa aggttggctc ttctcacttc actcataatg tgtccaggca
     5881 gaataaattt ctacaacttt attttcctca tcaactttgt aaaccactcg atgctgtcta
     5941 ttcagcctgc gtgagtatct gccttcatgt gtaggtctca gcttttcaaa agactgcgtg
     6001 ggcatatatg gatcttcctt caatgtctga atcacttctt caaattgttt ctttaaattc
     6061 gtttgcttga tttttcttaa atccactttc gcagagtttt tgattgcgac tgtatagtta
     6121 ctcatcaaag agcttcccaa tcaatatcat cgatattcgt aaagctactg tcgtcttttt
     6181 cgcggtctcg tactttatct aaagtacctg tttgctcaag aaaaagggtt tcttgaatcg
     6241 ctctccagtc ttccagccca atcaatacag cattgtttcc actacgatca ctaataatct
     6301 cgatttcagt atgattttca tttacctctt tcaataactg ataaaaattt ttacgagcag
     6361 tgctaggatt catcgttgta ttagccattg tctctcctcc tttttctgat ataattatag
     6421 cgtacttgat taagtacgtc aaaaaaatac aataaagcac ttaaattcag caaacttttc
     6481 ctctttttta catagagaaa aagacccctt tttagggagg atctttgtcc acgctcactt
     6541 cttactcctt tacgactagc ggtagccaaa cgtaaagtaa ttggtaacaa cgtatcatct
     6601 ttagcaaagg tcgaatccaa agcctgtgag tctatgcaac aacaccaata cacgaaggtt
     6661 cctcgttgca gtgtgacttg attgatatct tgaataacct gaggatacgc acgaaagatt
     6721 tgtaacggtt attcaaaact gattttggcc gtcaagtctt ctactgcctc aaaaatgatt
     6781 gcctctttcg tctcctgtca ttcatgttca ctatcgattt gatacttgct tagaccgttg
     6841 ggtttgtgaa tcaccaatta aaaatggcac tgagttatgg taacaaaaaa tggtcacttg
     6901 acgaggctga gattcctact tatgaagaca catggcactt aagagtcgag gtaaaggaag
     6961 aagctgaaaa tataggaggc tgtacattat ttggcaaagg attcggaaat tatgatcaag
     7021 acatcaaaat caaaatgttt tacagctaaa taggaagaat aataacattt catacgctgt
     7081 tcccatcttt aatggcttat agagggcacc tttagctgag aataagacaa aacagaccct
     7141 ctagcttcaa ttaagagcta gagggtctgt ttttgatttt aaaacttggt ttatttcttc
     7201 aaatggtagc tataggtgct gacaacgata ttttcacgcc aagacaatcg caatcttaac
     7261 cgcaaacttg tttggttatg gagaatattc tgccagcgtc tgtttggtac aaattgagga
     7321 atcaacacag tagttgtata gttttgtttc gctgcatttt tacttacgat atctacataa
     7381 cggataattg gattttcaat cgagcgataa gaagagtgga cgattgaaaa acggacatct
     7441 gggaaatgct ttttaaattc tgcttcgatt tctttttctt tttcgacatc ttcgtctagc
     7501 gaaacatgca tcgcaacgac ataatcaccg atcgaacgtg cgtaattcaa cgcaccgata
     7561 tttacccgtg tcacattccc gactaataca agaacggtat tcccgtcata atcatgtagt
     7621 tttgcatcat tttctaaacg aagctgttct gctacttttt tataatgatc atggatctta
     7681 taaaaaatga acataacgat tggcataatg atgaagaaag gccaaatgtc ccctaaccga
     7741 tagacaaaca aaatcgcaat gattgcatat gagatgaacg cacccgtaat attggcaatc
     7801 gatttgctta accatctttt tccttctttt ttccatttga cgaccatccc tgtttgagat
     7861 aaagcaaatg gaatgaacac gccgatagag tataacggaa tcaagcgttc tgttgagcca
     7921 tggaaaataa acagcaatac gatcgaacct agcgctagtg tgataatccc attggaatag
     7981 cctagtcgat ccccacgatc ttgatacatg tgaggcatga atttatcttt tgccagattg
     8041 tatgcaagaa ctggaaaagc agaaaaaccg gtgttagcag caactgctaa aatcagcgct
     8101 gtagcaaatt gaaggacgta atacaaaata ccatgtccaa agacagcttt accaatttga
     8161 gacaaaacag tgacttcttt ttcaggaacg atcccatacc aatagttgat aaatgtgatc
     8221 cccacgaaga agaaacctaa gatcaatgcc atcatcgtca atgtagcagc ggcatttttt
     8281 gctcttggct tcttaaagaa tggtacagca ttactaatcg cctccacccc agttaaagag
     8341 gaagaaccag aagaaaatgc tcgtaaaatc aaagcgatag aaacacccgg cacagccgct
     8401 cctggaagtg cagtagcttg caatggcaca gcacctgtca cgatattata taaaccaaca
     8461 acgatcagta acgagatcac aaggataaat gtgtacaccg gaaataatag gaacgatgca
     8521 gattccctca gcccacgaag attcagcatc atcagtagca aaacgattgt cacagaaata
     8581 gcgacttgat gaccgtagag tgctggcacc gctgatgtga tcgcttcagc acctgctgag
     8641 acagatactg ctactgtcaa catgtagtcg atcaataaag agccaccgga aatcaagcca
     8701 gcattctttc caagattctc actgctgacg acatatgctc ccccgccatg aggataagca
     8761 tggatgattt gacgatatga tagcgttaat gaaatcaata aaataataac aaaagcggca
     8821 atgggtagcg aataccagat ggctgcagca gacaaagcga ctaaaacgac aacgatttgt
     8881 tccgtaccat atgcaataga tgataacgca tcagacgaca ataatgccaa agcagcaaat
     8941 cgggttaatt tgtgctcatc attttcagca gattttaaag gcttacctac taacaacctt
     9001 tttaagtaat ccaatttcta aactccttta cacacaattt tacttcaagt tttatagtcg
     9061 aaaatttata gtaaacaaga tgttatagta cgctcactga ccttaaaagt caataaactt
     9121 taagaaaata attggaaaaa aacagaaaaa tatcaagcgc tttttcaaac gctttaatac
     9181 agcaaaaatc taacattttt ttatgggctt tttctttgaa ttctataata aaaggataga
     9241 acttgactta gaaaatcaag ttctatcctt tttgatatct taacttttgg aagagagatg
     9301 taaggctgct ttctacccat cattcttact actcgaagca tagcgcagtt gtcacaacct
     9361 cttgataaac ggtgtttaaa agcttgcatc tgcggtaata agcccaacca aattaaaaac
     9421 ataagaagct gtttttattt ggtcgttctt attacttgat gcagagcagc ttttttacaa
     9481 cctcttggta cacggtgttc aatcagctga ccctcggtat tagctcaaaa aaacggcaaa
     9541 atatgaggaa ctatttttcc atttttttct ctaatactca ggtcaaaaca ctgatttcac
     9601 aacctctttt tttaatgaaa aatatggctc tcatgcgccg cttctttgaa tttcgtcatt
     9661 ttttcttcga tgaacggata gattgccgtt cctacgattc caaagacaac aaacaaaccg
     9721 atcatgatcc agattgcttt cgtagcattt ggccaataaa ttcctcctgc agattcccgt
     9781 aacaagttta ccgcatgagt aaatggcaag aacggattga tcatttggaa gaatttccct
     9841 gagacttgga tcggatagtt cccgcctccg ccagagatcg acagtactaa gatgataata
     9901 gcgatccctt taccgacatt atcgaataat gcagccaaga catagacgat catcatgaac
     9961 gctaaggcga tcaagacagc aaatgacacg ctgtagaccg gattttggac atctactcct
    10021 agtaagaaaa tattccctaa tgtaacgatc aagctttgcg caatcgccat tgtcaaaaat
    10081 gtcagcatac gagcaacgaa ttgttctcgt ttcgtaaaat tgtttcggtc tttctttcct
    10141 aaatagtatt ctgtcgtagc aacacttgaa agaagaacag cgccgaccca cagacatagt
    10201 gcagtataaa acggggtgct agctgaaccg ttatttgcga ttggataaag cgcattggtt
    10261 tgtagctcaa caggttgtgt aaagaagtca ctttcttttg ttgcgtctag tttcaataat
    10321 ttgatgactt cgcctagatc gacttcttgt tcgccttttc ggatcgcgtc tgctgctttt
    10381 tggataccag ctttgatatt tggccagtca ttgttgatca agtccgcagc agcgttcaca
    10441 gctgtttcga cttctggcat tttttcattt actgttgtca atgtaccggt gatttcattt
    10501 tttagtgctg gccaatcatt ttggataaaa tcagcggcca atcctaattt ttgctgaatg
    10561 atcggtaatt cattgttgta taggtctgcc ccttcattga tgccattgac gatggtatcc
    10621 atatttccat tcagcattgt attggcatca tggatttctt gtccaattgc aggcatttct
    10681 ttttgatatt tttctaaaat tgccactgca ttcgttacag ttgctttcgt tgagctgaga
    10741 agcgaatcaa agtcgatctg gtccgctttg ctcagtgcgc cttgtgcagt cccaatcgta
    10801 tcgatgactt ggtctagtag agaagtgaca gtggaactga tttgacctgt attgatacca
    10861 gcaatgatgc cattgatatt attaacaacg gcatcaattt gatctagcaa ttcatcaata
    10921 gccttcgtgt cttcattttg aacagctgtg ttcaaagcag ataggcggtt ttgcagattg
    10981 cttagcaagg gttttaattg atttagctga tcaataattg gctgtaattt cgtatttcca
    11041 gcacttgttt gcaatttagt caggaactcg accagtttat cgattgcatt ttgttgttga
    11101 gatattccgt caataaagcc gttcaataca ttgctgatgt ttttcttttc atcatcagtc
    11161 agcttaccat cttcaatagc tgttcttaca gtatctatta acgaacctgt cgttgttgcc
    11221 acttgtccaa ttgacttcaa tgtaacatct acactgtttg tgatgcttgg tatcgcatct
    11281 ttcagctgag aggcacctga ttttgtttgg tttgctagat cacttgcctg attttctaaa
    11341 tttttcacat ctggcaagat atcttgtact tgttggatga tcgtcaagcc ttgttttgct
    11401 tcatcgattc cttggttcat tgtctcttgg atcgaagcaa aatcgccgtc gatctctgct
    11461 agttgcttac cggcattttg gatctctggg attttttctt gcaaatcaag gatgattttt
    11521 gcttgatctt tcaactcagg cattttgtca ttcaatgcaa cgactttttc acccatctca
    11581 tctactttag ggatgtaatc cacgaattca tttgctttat tcagtttttc cttgatttca
    11641 ggtagttgac tttgtaattc gaggacttgt tttgtgtagc cgtcgatcgt gtccaaattt
    11701 tcatctgttg ataaaatcat atctttaact ttattgatac ttactaggtt cgtatcgatg
    11761 tcgtaaccta tctcattgaa tactttcaat aaagtcgaac tggctgtttt gataaattca
    11821 tttgtgattt gcgattggat cgaacttgct cctttatcgg tgattttcgg tgcaatcgca
    11881 ttgatttttt catttaccgt atattcgatt tttggtttct tgatatcacc actagtaaaa
    11941 ctcagcagat cttctgagaa atcctttggc aagtaaatac ctgcgtaata ctttccggaa
    12001 cggactccgt cttctaattg ttcttttgaa tcaacgaatt gccatcctaa ttgtttattt
    12061 ttatgaagcg attcgatgac ttgctcccca atggccactt ctttaccttg aaactctgct
    12121 ggcttgtctg cactgtaaac agcaattggc agctctccag tatttccata tggatcccat
    12181 aatgctttga tgttaaacca cgcatatagc gaagggatga tcatgatcgc gatgatcaga
    12241 agtgttgcaa tcgggttttt gaaaatccgt ttccagtcaa gcaaatataa tcgaagcgta
    12301 tttttgattg actgcatagt tctccccctt cttctttttt cagctgaatt actttaccat
    12361 gtatttcata cttttttcaa tcggcttttt tcaaaaaagt cgaatttttg gacatatcac
    12421 ggcaattgtc tcattgtaat ctcttacata ttgagaaata attaaggatt acagccttta
    12481 ctttatcatg aaataaaagg gaaagataat tttatgccaa agtttttatc tattatacgt
    12541 atacgcaaaa aagctaaaac attttttgtc tttgccccta caaaaatgtt tcagcttttt
    12601 tcgtttttta ttcctgtatt tcttattttt cgaatgacag gatgattttc ccagcaggag
    12661 gatgtccttc aactagctga tgtgcttgga tgacatgctt aagtgaagca gacagattca
    12721 ttgaaataaa aacatgaaag gctccttttt ggtaggcttt gattatgcca gcaaaggctt
    12781 ctgcatctag atattccttt cttggaacaa acgattcata aaagccttcc tttttttgcc
    12841 gcaaatctag atctgggagg tcatttaatg caacatacct gccgcctggt ttcatgatct
    12901 ggatgccggt ttctcctttg atacttcctt tcgtcgcatc gattacaagg tctgcctgat
    12961 cagcaaattg cagacctgga tttgttttat cataagcagc aaatgctgaa gctcctaatt
    13021 tttttacctt ttcctcattt ttactggatg cactggttaa gattcggatt cccttttcat
    13081 ggagaagctg gatcagactt gacccgaccg cacctgatgc tcctagtatc attacagtat
    13141 ctgtcggctg gatttccgtc aaatggttga ttagattata agccgtaatt cctggcgtga
    13201 ccattccggc tgcttcttcc caactcattt tatctggaat ttttgctact tttgctgatg
    13261 gcaacacgac ttcttctccg taagtcccgc cgactgcatg gacagctact cgatcaccta
    13321 catgaacagt tgtgacatct gaaccaatct ctgtcacaat accagcgcca tcattacctg
    13381 gtacataagg gaatttcaat gttcgtatct ctttcatttc tcctaaacgc aaggcaacat
    13441 cataaggatt cacactgaat gcttttattt ctacacgaac atgtccagaa ccgacttctc
    13501 tgggatgagc atcgatttct tcaaaaacat cttgtgcttc tccgtaaccg ttaattgcaa
    13561 atcttcgcat ggtcaccatc tccctttttc tttaagttta cgtttatgcc tttattttga
    13621 caaaaaaaat gtcttttcgt tttggcgcat gattcaaaac aaaatagttg tcttttttcg
    13681 cgtaacgtgt taaattattt atctaaaagc gtaaagatta cgatttacgc taataaaaaa
    13741 taattggagg aatttgttga tggctaaaaa atcaaagatt gccaaagcaa aaaaacagca
    13801 agaaacgatt gaacgctatg ctgctttacg ccaggaatta aaagcgcaaa aagattacga
    13861 aggcttgcgt aaacttcctt tagatgctcg tccagaacga ttgaaaaatc gtgatttaat
    13921 cgatggacgc cctcgcggat acatgcgtaa attcgggatg tcccgtataa aattccgtga
    13981 attagcacat aaaggtttga ttccaggagt aaaaaaagcc agctggtaat ccaagtctgg
    14041 ctgtaatgta aaggaggagg gcgcatggac cactccttgg atcatcaaaa aagatacgtt
    14101 cgcttcttct agcagaaatg ctggtactag aagcagacgt atcttttttt acgcacagct
    14161 ggtccttttt tatatgtaaa ggctgcatgg ttacttcgct atatttattt agtaacagga
    14221 gtcagtgctt cgatacccac actaattttc aatgctcggt caacttctgc cataatgggt
    14281 cgatccaaat ggcagacctt ttctttcaat cttgatttat cgatcgttct gatctgctcc
    14341 aacaggataa cggagtctct ttctattcct gttccagtag ctttgatccc aatgtgagtc
    14401 ggaagcttag gtttagccat ttttgccgtt atggccgcaa caatgatcgt cgggctaaaa
    14461 tggttaccta gattattttg aataaccaaa actgggcgtg ttccgccttg ctccgatccc
    14521 acgactggag aaagatcagc aaaataaata tctcctcttt ttaccatagg atcctcctta
    14581 ttcttgatat tctcttggta tccgctgtcc taatccgcaa gccacttcat agtggatcgt
    14641 acctaattga tctgctatat cctgcatagt gatttctttg tctgcatttt tgccgataag
    14701 cgtcactttt gttccgacag gaaattcttg aggcaatcgg atcatcagtt gatccataca
    14761 tacacgccca atcgtttcac agtagttccc ttctaccaat agagaaaaac cctgcatctt
    14821 tcgtggccag ccatctgcat aaccgatcgg tatcgttccg atccactctg cttcaggagt
    14881 gatatatgtt tctccatagc cgattccttc acctgctggg agttttttga cttgaatcaa
    14941 ttcagaaacc agctcaagtg ctggctgcaa tggataaact tccggtagtg cgtgcccaga
    15001 gggattaagt ccgtacattg ctactccata acgaatcata ttgccgattg tttcatcatg
    15061 ccataaagcc gttgcactat tgctgacatg tacatatcga ggaagttcgg gaagtttttt
    15121 cagcacttct ataaagcgtt ctttttgcag attccaatat gtatcatctg cctgatctgc
    15181 tgtagaaaaa tgggtaaata aaccttccca ttctaataca cgactttctt tgatgaatgc
    15241 agctgcctct tttacttctt caggtgtaca aaatccgata cgacccatcc cagtgtctgc
    15301 ttttagatga atcgaaagag gcgtctctag cttacttttc tctaaaacag cagctgctgc
    15361 ttgcaaccat tcccgtgtac ctgcagttac tgataaatca tgagcaacag ctacagtcag
    15421 tgcgtcgtaa ggaaccatat tcaagataag gatgggctga gtaaatccag cttctctcaa
    15481 ctcgactcct tcatctaaat ttgaaacaca aaagcccgaa gctccacctg caactgctgc
    15541 ctcagctgtt tcaatagctc catgaccgta tccgttcgct tttactactg caaacaattc
    15601 ttttccttgc ggaagtcttg caacttcatt tgctacattt tcagttatag cttttttatg
    15661 tatcactgct ttcgttgggc ggtgccaagc gacaaccatc gtttttcctt ctttctagtc
    15721 ttccaaaata atttgtgcaa aagctgtttc atttgtatga gtaatactga cccatacatt
    15781 gccttcatga ggtgaacgcg tcacaacagg ctgaccattt tcatttttta aaatctcgac
    15841 atcttgaaag gtaacttttc cgatcccagt tccccaagct ttagagaaag cctctttgca
    15901 agcgtaacgc ccgcctaaaa attctacttg gcgatgaaaa ggcaaggact ggaatagttt
    15961 catttcatca gaggtcaaga ttcgtgcaat gaattttggt ttttcttcga tcagccgagt
    16021 gatcctagga agttcgactg catcgatccc aattcctttt atcatatgct tttctctttt
    16081 caataaattt agaacactct cattctacca aattataacc attttgccac aaataagtcc
    16141 acaaataaca aaaatgaaag tttctcttaa aaaagagaaa ctttcatttt atgtttaatc
    16201 gttatttgaa cggattacaa atccgcgttt tttatcattg tgacgtttac cgccatcttt
    16261 ttggctgtta ttatttttgc tataaccgcc ttttgatttg tttccacgat aattaccgcc
    16321 gtcacgacga ttacggttgt ttccgccacc acgtttgcca tttttattgt atcctttttt
    16381 ctgcattggc aatgggcgtt ctggtgtgat cttcactggc actgcatcag ctggatcttt
    16441 tgctgtcgtt tttagcaaaa gtgcaactaa atcttgtgca gaatattctt ctaataattt
    16501 atctgctgtt tgcaggtatt tatctagacc attttctgca agttttgttt cgatttgttc
    16561 aactgcagca cctaattgac ctttgaatgc ttctttttcg gttggtggac gtaaagtcgt
    16621 catccgtttt ttcgtcaagt tttcaatcac atgcaagtag tccatttcat ttggtgtaac
    16681 gaaagtcaca gacataccgc cttttccggc acggcctgta cgtccgatac ggtgaacgta
    16741 gctttctgga tcttgaggga tatcatagtt gtacacatga gtgacacctg aaatatctaa
    16801 tccacgtgca gcaacgtctg tcgctacaag gatatctaaa tgaccgctct taaatgaacg
    16861 caatacgctc attcgttttt gttgagacaa atcgccatgg attccttctg cacggtaacc
    16921 gcgtgcttct aatccgcgtg ccaattcgtc tacacggcgt tttgtacgtc cgaagacgat
    16981 tgttaattca ggtgtttgaa catcaaacaa gcgagtcatg atatcgaatt tttcgtattc
    17041 ttttgcgcgg acatagtatt gatcgatcaa gtcagctgtc atttctttcg ctttgatctt
    17101 cacatgatgt ggatttttca tgaatttaac gccaatattc ttgatagctg gcggcatcgt
    17161 tgctgagaaa agtaatgttt gtctttgatc aggaacttgt gaaatgattt tttcgatatc
    17221 ttccaagaag cccatattca acatttcatc tgcttcatcc aaaactaatg tttgaacggt
    17281 tccaagtttc aatgtgtggc ggttaatgtg gtcaagcata cgtcctggtg tacccacaac
    17341 gatatgtgga cgatctttca agccacggat ctgacggcca atatctgctc cgccgtaaac
    17401 ggcttgtaca cgtacttttt tgtcgcgccc taaacgatat aattcttctt gtgtttggat
    17461 agctaattca cgtgttggtg cgataactaa accttgtaat tcgtgacgat ctggatcgat
    17521 tttttctaac attggtaatc caaatgcagc tgttttccct gtacctgtct gtgcttgtcc
    17581 aataacgtct tttccagcta atgctagtgg aatcgtttct gcttggatag gcgttgcttc
    17641 ttcaaaacca gcgcgttcga ctgatttcaa caattctggt gataattcta gttctttaaa
    17701 tttcaaatga atcctcctat atggttgtta actgaggatg tagctttttt gctaccccgt
    17761 cgatttgttc ggtcaaagtg tattgtttct ttttgacagt ggccacgcac gtttttatcc
    17821 attcttagcc cctgtggcac caccgaaaaa tcactttgcg aagtttaggc attagaaaag
    17881 ccaaacgggt cgcgttcagc tttatcatta ttatgacatc tggcttcaac aagttgtttc
    17941 tatggcaaaa ccagtccaat ttctcttcat ctttcggcaa taggttattc taacatagtt
    18001 tttcgttttc tgcaagttta cagattttta tttcatttgc tgaagttgtt ctaccacttc
    18061 aaataagccc atgctgttgc tgccttttag cataatacta tctgttgctt caagattttg
    18121 tttgatagca tcaatcaatt tattcttttc tttttcaaaa tagaacacag gtagttgggg
    18181 gtatttttgg ttcaattctt cttttagtgc atgcatttgt tccccataaa ggaaaatcat
    18241 ggcgaatttc tcaggatcga tatgttcact catttgcgca tgcatattta aagactcttt
    18301 ccctaattcc agcatatctg ctaacacagc gattctgcgt ccttttgttg gcagattttt
    18361 aaaagtatct aataccagtc ccatggctgt tggatttgca ttgtagacat cgctcaaaat
    18421 agctgcacca tttcctgccg ttagccattc ggttctgttt tgagtgatag aaacatgggc
    18481 gagcccttgg cgtatctcag gtatggacag tccaaagaag cgcccgatcg tatacgcaat
    18541 caaagcattc gttacattat aacttccagg cagcgggatc gtaaatgttt ctccttctac
    18601 tacaaaatcg gtttgtgctt ttccttttgc tgtcacttcc gcatgaacat cgccatttcc
    18661 aataccgaat gttgtgactg tttgtactaa tgttgccgtt aaaggtgtca gcaacggttc
    18721 atctgcgggc acaacgagta atccgtctga tacaagtccg tcagtgattt ccattttggc
    18781 tttagcgatt ccttctcgcg acccaagatt ttctatgtgt gcttcaccga tcattgtgat
    18841 tgctgcagct tttggttgag ccataagtga aagttccgtg atttcattgg cgtgatccat
    18901 gcccatttcc aaaacgatct tttctgtatc ttggggcatg tgcaaaatcg tgtaaggaag
    18961 accaatatta ttgttgtaat ttccttgcgt cttatatgtt ttgtattttt cagacaagac
    19021 agacgcggtt agatctttcg tggtggtttt accattgctt cctgtaattg caacgacttc
    19081 tgcgcctact ttttccaagt aataggttgc caattgctgc atggctttca aaggatctgc
    19141 tacaggaata actggaaaat tttttggagc ttgttcaaca ggcaagctcc aaaatgaagc
    19201 agatgctccg tttgtgatcg cttgctggat aaattcatgt ccatctcgcg taccagcaag
    19261 aggaacgaat aaacttcctt cggttatctt ccggctgtca aattcgacat tggtaatctt
    19321 tgtcagtgca gagcattcgc tgcccacggc ttgtgcaatt tcttgtattg ttagattcat
    19381 tgagatctct cctaaaaaag gttgtgaaaa aagcgtttgg cctaaacggc gggttcgcat
    19441 gactctcact tccaagtaaa tttctctatt cgtatttaga agctagaatg aacgcggtta
    19501 cgcaatcact ccagcctctt ctttatttct ctttattcat ttttttcaaa aaaagattga
    19561 cgctgatggt atcgattcat tcctaactgg atcagttctt caatcaaatc accgtatttc
    19621 aagcccatat tttcccataa gagtgggtac atactgaact ccgtaaatcc tggcatagag
    19681 tttaattcat tcaggaataa ttcattttta tttgtcaaaa agaaatcgca ccggctcaat
    19741 ccgcttccac ctaacatcgt gtaagctaac ttcgcgtact cttgcgcttt ttgataaact
    19801 tcttctggca cttcggctgg aatctgcatt tcgattttat tattgatata ttttgcttca
    19861 taatcataga atgctacgtc ttttacgact tcaccaggca aagtcgtccg aacgtcttca
    19921 tttcctaata cagcaacttc gatttcgcgc gcttcaattc cttgttcaac gattgctcga
    19981 gaatcatact gataggctgt tgctaaagca ttttgcagct cttctcggtt ttctgctttt
    20041 gtaatgccga cactagaacc catattcgcc ggtttgacaa acatcggata aagcaaagaa
    20101 ccttcacatt gatcaaatac ttttttagga ttttctttcc attgattctt aagtactggt
    20161 acataaggaa cttgcggcac accagcagct tgtaaaatat acttggtcat gattttatcc
    20221 atggcacatg cactggtcaa tacgcctgcg ccgacataag gcatattcaa tgtctctaag
    20281 aagccttgga tcgttccatc ttccccgttt ggcccatgta aaactggaaa aacgatggct
    20341 ccttcttctt tgatttcgcc cggattgatc acttttcctg taaagccttc ctctgtctgt
    20401 ccacttgggt cccatgaaag atgcaagaca tctttgctgg caggtttttc tgttagtaat
    20461 ggacctttga cccattgtcc ttcttttgta ataaatacga gttgaacttg gtaataatta
    20521 taataaatgg catttaaaac tgaaaatgcg gaaagaatgg acacttcatg ctctgcgctg
    20581 cgtccgccat atagtaaagt aatcttcaaa agattgtcct cctgtattct aaatcgttca
    20641 gtgatttact ctcatatttt agcacaaaac aaaaataaac gaataactta tttataaaaa
    20701 aacgagccag cttctaaaag aacgagcgtc atttattttt tgtcagctag attatttgtc
    20761 ccgtttttta taaaaagtta tgataaagaa aaggagggat gatgatggcg caaacaaatc
    20821 cacgcagccg cttggcagaa atcattcgag tgttcatcaa ttacaatgta gtgccaaact
    20881 ttgtacagca aaaaaatccc gaacaggtaa aaaaagcctt tgaagaacta ggccccacct
    20941 tcatcaagat aggacagatg ctttctgttc gagaagattt gctttcttct gccttcactc
    21001 aaacattcaa aacattacaa gatagtgtac ctagcgacac attttcaacg gtcaaaaaga
    21061 cgatcgaaac agaactttct ctttcattat cagatatatt cgatgatttt tcaaaatcgc
    21121 cttttgcctc tgcttctatg gggcaggctc atcgtgccaa gctgaagaac ggtgacgcgg
    21181 tagtcgttaa gatccaacac cctaacatcg ccgaagagat ccgacttgat cttcaactat
    21241 ttgaacgagc gatccctttg atcaaataca ttcctgaaac tagcgtagtt gatctaaaag
    21301 gggtactcca agaagtaaaa cgttccttga tcaatgaaat ggacttctta aaagaaagtc
    21361 aaaatggcga acaattctat caaaagaaca atggctggaa agagatccgc agccccaaga
    21421 tttatgacgc tttttgttcg aaaaaagtga tcgtgatgga agaaatgtct ggtaaaaact
    21481 tgaatcattt aatgaacgcc gaaaacaaaa ctgaaacttt tatcactgga attcaaaaca
    21541 agcagctgaa acaagaagtt gcaaagcttt tagttgagaa ctttatgaaa caggtgtttg
    21601 atgatggttt tttccatgct gatcctcatc cagggaatct gctattccat gtactaacaa
    21661 aagaagaaca gacacaagct agccgaaaaa ctgaaacagt ccatgaaaaa gagttcggtt
    21721 cctttgcttt tcgagcctcc acttctgcag aagaccctgt tgcgccatat accatcaact
    21781 acatcgattt tggtatgatg gggcatcttt ctgcaggact taggcaaaaa ctcacacaag
    21841 ccgttttagc gctgtatacg aaagatgctt atcgaatcga aaaagcggtc cttcgacttt
    21901 gtcagcaaga aggctcgttt gatgaaagtc ggttccacca agaactgaca agttttcttg
    21961 aacagtatta tgattctcct attgatgaaa tcaacttgca agaagtattc acacatgtcg
    22021 tgacgatttg tcatcaaaat aatctgcagt tcgatcgtga tatcacgctt ttgctgaaag
    22081 catttgggac attagaaggg gtgatccgcg tcctcgatcc tgaagtttcc ctcatggaag
    22141 tcgcttcgcc ttttgctcaa cattattttc tcactcatct ggatgtagaa gatacattga
    22201 aacaaagcgg attagactta ttggaaggga tgaaggctgc gcctaagatc cctcaacagc
    22261 tccaccatct attagaaatg tggacttcgg gacaaggaaa agtcaatttg gaattgaaaa
    22321 agcaagacaa attattgagc aggatcgaaa gtatgatcaa tcggcttgtt ttcggtatga
    22381 ttttagctgc attgatcgta gggtcctccc tgcttgtcca agcagcacct gtagaaaacg
    22441 cagaggtcgt ttccttatta gggatattta cgtatgctat tgccgcattc gtcattattt
    22501 ttttggcaat cgatgcattg atccaaatgt ataagaagag gaaaaagtag aaaaaggttg
    22561 tgacagaaag aatcagcttc aagaaataag aaggggtttt acataaattg tttctcaaat
    22621 ttttgtgaaa attcggctta tttttcgaag aggctacttc tgaaacaccg agaataaaaa
    22681 atgtgaaaaa gctgttctgc acccagtaat aagaacaatc aaaaagggaa catggaacac
    22741 atttatcctg tttccctgtt ccctttttga tgtattcgag cataaataaa ataccttcgt
    22801 tacctactat cttctgtact tggtaaacct tagtcaattg ccttttttaa cgcttgacga
    22861 aaagacatat gatctttctc cgtaaagggt tttggaccct ttgtccttat gccagctttt
    22921 cttaactttc cactatgata acgttgttct agaagcgtgc cgatattcgc ttcgtcaaat
    22981 tcttttccag agtgatggaa cactcgcaca cccttcggca atacaagtga agctaaacca
    23041 taatcttgcg tgatcaaaaa gtcattcttc ttaaccatac cgacgatttt ataatccgca
    23101 ctatcagctc cgcggtctac gtagacgatt tggacatgat tagcgtattc tttggtcgta
    23161 taatggtcca cactggtcac gataaccacg tcaagaccat aggattctcc taattttatg
    23221 acatcttctt ttactggaga accatctcca tcaatgaata gcctcattct ttttcgcctt
    23281 cttccataaa tgataaacag ttactaatcc gctcagtaaa agaccaacat tgatccattt
    23341 tgccaagata agcagttgta cactttctat cggcactgat tcatggttca gcgtgatcaa
    23401 taatagatgg attggctctc ccatttcttt tgctaaaaca aacaaaagcg tcatcaataa
    23461 aaactggtgg atcacactcc ctttattttg agtagtatac ttgacaacga tccggcgatc
    23521 cattggaata gctgtatcag tttgataata ttccttattt agccagctgc ctgttactac
    23581 gtgccagaaa ctaagcagaa agacacctat cgcaaaacca tttggaatca agaggaccaa
    23641 taaagaagaa ctcagtccaa gcaattgcat agccaatgga gaccatgtta aacgtttcaa
    23701 aatccatggt ccgactattt ttgctgcaat caagcctgct gcataaggca aatacataaa
    23761 aatcgaaagt ttttctaatc cgtacagtat gccaacataa aaactgctaa ataaaaaaag
    23821 gaaattccct agcattccat ttagaaaagt aaacagattt agccagcttg gcaacttcca
    23881 ttccttcttc tggtgtccta aaacaaacac gaacaagatg aacagcagca agaaaccata
    23941 aatagcaaga tcaaaaagcc tttcatcgaa taacaatcgg gcatttctca aaagaaacaa
    24001 taagccaaag aatatcaaaa ataaaacaag ctctcgcgta gctaccagat ttcttttgat
    24061 gtctttgaaa tccagttcgt aacgcggata atgactaacc gtatggtagg ccatgacgta
    24121 aaaaagtgta taaatagtaa agcttgctgc aatctgagtc ggtgcaggca actcaatcgc
    24181 ttgatacagg agaaatagcc agatagctgc aaataagtac tttttcccat ccatattgat
    24241 aaaaccttgc gtcttttcgt gaaaattgac cgtggtatta gcaggtacaa gccatgctgc
    24301 actcagccct agcaaaacac ttgaaaaata aaataaagga aaataaaata cgcctaatat
    24361 tccaagcaat gaaccagcac caccaatcag cagagcgatc atcagcaacg tgtaactatc
    24421 tagaccaaat cgtagactgt tgactaaaaa tagtccagtc atccgaaagg cataaaacag
    24481 cgtaaatggc aaaatcgtca gccaattatg cgagtctgtt agtcccaaaa ataaaagata
    24541 aggaataaaa ataaccgtgt tacttaagat aaagggcgtt gtttgtgtaa aattacgccc
    24601 aaatcgtttg agtttttcga acatgacaat ggctccttaa acagtcttat aactaatctg
    24661 ctcttcgatt ttagcattaa aggggaaata aaccaatttt acagaatata ttcttgtaaa
    24721 aaaaaataca gaaagaagca taagcttctt tctgcatttt tacggtactt gattactcaa
    24781 gcagacaagc ccttctccac aaaagtcttg cccgacaccc ctgcatccgg caggtgataa
    24841 agatagaaat caattatcct gtgcctccaa aatcaattag gagctctgta aaccagagtc
    24901 cttctcgacc cgtcgcataa atttacttta acacagttcc tatgtttatg cgagagcttt
    24961 tttcatttta taatcgacca tttgaaatcc ttgaccgata tctgaaataa gttcttctgt
    25021 aatctcaaat ccttcatgct tataaacctc gattgcttga tgattcccct gattcactcg
    25081 caaaaagatc tctctttcct ttgtttgcgc agcaagttct tcataccagc gaaaaactga
    25141 agaagtcaat ccttgaccac gtactttatt tagcaaatat aatttactta aataaatttt
    25201 tccatttatc tcttcataag cagtgtaacc gacagtcttt tcttcgcttt ttaataaaaa
    25261 gtaggacaca ccttctgtca gttctttttg aatctgtttt tttgactgat aagttttcag
    25321 catgtaatcc acttgttttt gaccaatgat cggcacaaaa acttccggcc atacttcttg
    25381 tatgactgcc atcagctcat ccagttcatt ttctgttact tttatttttt ccatacaccc
    25441 acgtcctttt ttacattata aatgaccttg cgcttgatgg aataaaaaga gcatgatcat
    25501 gctatacaca cccaccgacc acggtttcaa gaaaatcaca atctgcataa attccctaaa
    25561 agataatttc gtcaaaccag ctactagaca gatcaagtcg tctggcgcaa agggaagcag
    25621 caaggtaaca actagtactt ttttaatatc tttcgttttt cttgtcagca attcatcaaa
    25681 tttctgatac ttttttggcg ataaaataag ctggacaaag gctacgccat agtaacggac
    25741 aagcaaaaat ccgattgctt ccccaatcac taggccaata caactatata aaagcccaaa
    25801 tccattgcca aacatcagca tgcccgctac tgaggagatc cctcccggca aaataggaat
    25861 gatcacttgg atcacttgca acagaacaaa tatcatcact gcatacttgc caaattgctg
    25921 gataaacgct tgcaaagacg tgctcgatcg aaaaatccca tgatgataag cataaagaat
    25981 caacacaaag aaaaaaaaca gaccaataat gggcatgatc tgaagacatt tttgtaaaac
    26041 tgatcctttt ttagacatct atattcccca catctttttt catttattaa aactgcctcc
    26101 tt
//
LOCUS       DACCXO010000030        25569 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.031, whole genome shotgun sequence.
ACCESSION   DACCXO010000030 DACCXO010000000
VERSION     DACCXO010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.031"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            152..280
                     /locus_tag="IVY70_001163"
                     /pseudo
     CDS             152..280
                     /locus_tag="IVY70_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
     gene            394..633
                     /locus_tag="IVY70_001164"
     CDS             394..633
                     /locus_tag="IVY70_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958302.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            745..939
                     /locus_tag="IVY70_001165"
     CDS             745..939
                     /locus_tag="IVY70_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958303.1"
                     /translation="MEEKLLKTMKQKHLKRLSVMQYINDMQITGKEKACLLGSMKNFE
                     QLRRTYVKTSSNCQLLLEVS"
     gene            1025..1696
                     /locus_tag="IVY70_001166"
     CDS             1025..1696
                     /locus_tag="IVY70_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP7958304.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQQEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1749..3725
                     /locus_tag="IVY70_001167"
     CDS             1749..3725
                     /locus_tag="IVY70_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7958305.1"
                     /translation="MAINRYVKKAGMLIGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            3740..4492
                     /locus_tag="IVY70_001168"
     CDS             3740..4492
                     /locus_tag="IVY70_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7958306.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTILSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLESNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            4508..5263
                     /locus_tag="IVY70_001169"
     CDS             4508..5263
                     /locus_tag="IVY70_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958307.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKNRVKKLSSDQLKLVSNEETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            5276..5536
                     /locus_tag="IVY70_001170"
     CDS             5276..5536
                     /locus_tag="IVY70_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958308.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            5533..7623
                     /locus_tag="IVY70_001171"
     CDS             5533..7623
                     /locus_tag="IVY70_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7958309.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            7650..8114
                     /locus_tag="IVY70_001172"
     CDS             7650..8114
                     /locus_tag="IVY70_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958310.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            8111..8353
                     /locus_tag="IVY70_001173"
     CDS             8111..8353
                     /locus_tag="IVY70_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958311.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            8371..8673
                     /locus_tag="IVY70_001174"
     CDS             8371..8673
                     /locus_tag="IVY70_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958312.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            8743..10806
                     /locus_tag="IVY70_001175"
     CDS             8743..10806
                     /locus_tag="IVY70_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958313.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKYEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            10806..13418
                     /locus_tag="IVY70_001176"
     CDS             10806..13418
                     /locus_tag="IVY70_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP7958314.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            13415..15948
                     /locus_tag="IVY70_001177"
                     /pseudo
     CDS             13415..15948
                     /locus_tag="IVY70_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321282.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15998..16330
                     /locus_tag="IVY70_001178"
     CDS             15998..16330
                     /locus_tag="IVY70_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958315.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            16331..16924
                     /locus_tag="IVY70_001179"
     CDS             16331..16924
                     /locus_tag="IVY70_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958316.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            17087..19111
                     /locus_tag="IVY70_001180"
     CDS             17087..19111
                     /locus_tag="IVY70_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP7958317.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            19111..20310
                     /locus_tag="IVY70_001181"
     CDS             19111..20310
                     /locus_tag="IVY70_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP7958318.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            20326..20970
                     /locus_tag="IVY70_001182"
     CDS             20326..20970
                     /locus_tag="IVY70_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958319.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            20981..21787
                     /locus_tag="IVY70_001183"
     CDS             20981..21787
                     /locus_tag="IVY70_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958320.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            21809..22039
                     /locus_tag="IVY70_001184"
     CDS             21809..22039
                     /locus_tag="IVY70_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958321.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            22553..22861
                     /locus_tag="IVY70_001185"
     CDS             22553..22861
                     /locus_tag="IVY70_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958322.1"
                     /translation="MEVRIRNVDEKIVREIDRRCDELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQDKFDQLLNQLIDKFSFVQSHNTKVLEEYICQNNQIIELLIEQSRGGEV
                     "
     gene            22863..23285
                     /locus_tag="IVY70_001186"
     CDS             22863..23285
                     /locus_tag="IVY70_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958323.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     SEVEKKVNSNQAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            23305..24894
                     /locus_tag="IVY70_001187"
     CDS             23305..24894
                     /locus_tag="IVY70_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958324.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTGKEFSETSNEAQSLMKHGINFDKSEEQDFTK
                     YLSYMTRHYALEQKKVLTHSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQTFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLTEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            24906..25241
                     /locus_tag="IVY70_001188"
     CDS             24906..25241
                     /locus_tag="IVY70_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958325.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            25382..>25569
                     /locus_tag="IVY70_001189"
     CDS             25382..>25569
                     /locus_tag="IVY70_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958326.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         9544 a         3962 c         5257 g         6806 t
ORIGIN      
        1 agatcaaata aacaagaacc gtaggaacta cggggatagc ttggtaaata agaggcacct
       61 ctgttagtaa agtaatacgc tatcaagtat gctctcttcc caagaagctc ccacttcaag
      121 cgtaagaaac cgttaggttt agctaagtgg tgagtagttc acaatgacgc ttacgaatga
      181 taatggagac gaattatctg ttgaagatac ttggtcatat ttgagtgatt atattgtcag
      241 cgttgaaatc attgaatttg tagcggataa agaagaataa aaactggagg aataaatttt
      301 acagataaag gcttagaaga agtcacgacg aataataaaa aggtagtatt accttaggca
      361 ttgagtaagg aattcattta ggaggaaaat tagatggcgc gaccagtttt agaggcgatg
      421 gatatggata ctttgacaaa aggaaggtat gtacagctgt tagctcgtgg ttattcacct
      481 aaagatgtat tcaaatccct gtctaaagga acagatatgg aaaaagaaag attacgaaaa
      541 gaattcgatt attggagaac ccataatgga atcaaggatt tgaaaccagc acggccagta
      601 attaaacgaa agaaaaagat aacccaagac tgaagtgtag agcgtatgtg atcataggct
      661 tttttatttt tgcaaaaaac aaggaaggta ggaaaactca tgaattatga agaaatcgaa
      721 aacagaaaaa aagtgtcgaa ataaatggaa gaaaagttgc taaaaacgat gaaacaaaaa
      781 caccttaaaa gattatctgt catgcaatat atcaatgata tgcagataac aggaaaagag
      841 aaagcttgtt tgttaggttc aatgaaaaac tttgaacagt tgagacgaac ttatgtgaaa
      901 acaagttcta attgtcaact attgttagaa gtctcatgac attcattaat agaattcatt
      961 tttaaaactt tttctatgga aatgagaaaa agattttttg ttttgtctga aagggggtag
     1021 ctggatgaga aaatggttat ttggtttttt aggagtcgct ttaatcgtct tttgcagtgt
     1081 gtttggctat gttagctacc aaaaacatga aggagaggtg tttaagcaaa atatagaaaa
     1141 aaagatgccc gtagatcaaa tcaatgcaca tgcaaagagt tataaggagg atgcaacgaa
     1201 cgtcaataat gatatgtctt tggggcaaat gttgtccata caacaagaag caatcgaaat
     1261 gggcgtcaat aaacaagtat ttgcacaaat tcagattcct gcacttggtt tagcattgcc
     1321 aatttttaaa ggggcaaatc aatacacttt gagtcttgga gcggctactt atttttatga
     1381 agatgctgaa atgggcaaag gaaactatgt cctagcaggt cataacatgg aaatgccagg
     1441 tgttttattt tctgatattc aaaagttgtc attaggggag gtgatggact tagttagtaa
     1501 cgatggcgtc tatcgttaca aggtaacgcg taaatttatc gtacctgagt actttaaatt
     1561 aatcgatggc gttcctgaag agaactcttt cttgtctttg ccaaagaaag gagagaaacc
     1621 cttattaacg ttgtttactt gtgtttatac gtcacaagga aaagaaagat acgtggtaca
     1681 gggagaacta caataacaag gttttaatca acgtgagtcg ttgaaatatg aaatgaggag
     1741 agtaaaaaat ggcaataaat cggtatgtaa aaaaagctgg aatgcttatt ggaatgttag
     1801 gaatcattgc aggctgcttt cagggatatc gagcatatgc agaagatgtc acgcaaaaaa
     1861 cgccaccaga gaaggtcaat atcacggttc ataaattaat gtacgatcaa ggaacgcaat
     1921 tgaatgttga tattgacggg atcaaaaacg atggttatac gcacgatcaa tatcctacag
     1981 gtgtaacgaa atacaacaag gcagattatg gtgatgttga gtttacgtta ggaaatatta
     2041 cggatcaagt attaccgaga gaagattctg atctaaccaa cgcgaaagtg gacgagattg
     2101 tacaagatgt agaagacaag ggatctaaca gtgagtacgt caagaatgca accaacaaaa
     2161 ttacttcagc tgtagatgaa aatggtgaga tcacttttac tgatcaacca gcctatgtca
     2221 attccaaagg gaatgtatat gtggtttatg aaagtaaatc cgctgctgga ttagtcacac
     2281 aaaaagcaaa accaatggtt gtgattgcgc caatgacaga taaaacagga tcaggatttt
     2341 taaaaaatat tcatttgtat cctaagaaca ttgtttctaa gttgagtttt gaattaacga
     2401 aatttggtga cgatggcgca gaccaaagca aacaaaccct tttaaaaggg gcaaaatttg
     2461 agttatacaa gggcgaacct ggaaaaggaa caaaattggg tgatcttgta tcggatgatc
     2521 aaggaaagct gactgcgact gatttgacgt taggaaaata ctattttgtg gaagttcctt
     2581 cggaagttgt agtgggatca gatcaagaac caacagcaga tcaatattta ttaggcgcag
     2641 acgctcgcaa cgatgcgcac aataaattaa cctttgaaat tacgaatgat ggcgtgacaa
     2701 gtgacttaaa agcttcatac gtaaactata aagcaccagt gatcgataaa acagtaacaa
     2761 atggaacagg acaagaacat tctttccaaa ttggtgacgc agtaaattat caaggaacga
     2821 ttcatattcc aacagacatt gcaggtggcg cagacggtat cacagtaaac ggtgtgaaaa
     2881 gtgaaacttc tccttacagt gtatttaaat ggggagatac agcaggacag ggactttctt
     2941 atgtcgctgc taaagcaaat atcaaagtaa caaataaaga tggatctgtg gtattaaaag
     3001 aaaatacaga ttataaaatc caaaattcag aaaatggctt tgtgatcgat tttatcgtga
     3061 ataatggcca agtaagtgat actgtagcaa gtttacacgg acaagatctt caaatgactt
     3121 ataacatgta tgtaaatgac agcgcagctg ttgccaatcc attaacaaat agcgtggatt
     3181 tcgtttataa caacaatcca tttaatcagg aagaacacca cgaaaaaacg aaagctgacg
     3241 ttgtgactta tggtgcaaaa ttcttaaaag tcgatagcgg actatttgga acaggaatca
     3301 aagcaacacc gcttgaaggc gcagaatttg ctgctaaaaa cgcagaagga aaatattatg
     3361 gtggattagt tgatacggat aaagacggtg tcaaagaagc agtatgggta gacgatgtag
     3421 caaatgcagc gatcctaaaa tcagataaag aaggtcactt tgaaatcact ggtttaactg
     3481 aaggtgaata ttcactggaa gaaacaaaag cgcctgaaaa ctatcaaaaa ttaacgaaag
     3541 agatctcttt caaagtagac aaagattcat acaaagaaga aaaccgaatc acgatcaaaa
     3601 acaatcaaaa agcttccgta ccaatgaccg gtagcaacgg ttttcaaacg tatgtattaa
     3661 tctcttgctt gttgctagga gcaggggcac tttctgcagt tgtttacttt aagaagaaag
     3721 cctagaagga tcaaaaccaa tgattatcag gaagcctaag aaaaagcgaa tcatgggtaa
     3781 atggatcatt gctttctggt tactttcagc tgtaggtgtc ttacttttga tgcctgctga
     3841 agcgagcgtg gcgaaatacc aacaaaatca gcagattgcc gcaatcgatc gaacaggaac
     3901 ggcagcagaa acggattctt ctttggatgt tgctaagatt gagttaggcg atcctgttgg
     3961 tattcttaca attctaagta tttctttaaa actaccaatt tatgacggaa cgtccgacaa
     4021 aattttagaa aatggtgtag gtattacgga aggaactggt gatattacag gaggaaacgg
     4081 taaaaatcct ttaatcgcag gtcatagtgg actttataaa gataatcttt ttgatgattt
     4141 accttcagta aaaaagggag aaaagtttta tatcaaagtt gatggtgagc agcacgctta
     4201 tcaaatcgat cggattgagg aagtgcaaaa agacgaacta caaagaaact ttgtcactta
     4261 cttagaatca aatcccaatg aagatcgagt aacgctaatg acatgtacgc ctaaggggat
     4321 caacacacac cgttttctag tgtatgggaa gcgagtaacc tttacaaaat cagaattaaa
     4381 agatgaagaa aataagaaac aaaaattatc ttggaaatgg cttttaggaa gtactgtttt
     4441 tctttcagtc atgatcattg gttcgctatt tgtttataaa aagaaaaaat aaaggagggt
     4501 aaccaacatg agaaatagaa aatggctatt tgtttgtgca gccgctcttt tttctttcac
     4561 tgcctacaca cagttgacgt ttgcagacag acaagaaagc acggatagca cagaacaaac
     4621 aatccaaacg tcagatagca gcacgattca aagcagcgat gaaaccgtcg attcgtcaac
     4681 agttgattct tcagaagaac ctaaaattgc tgaagacgat caaatagtca aacagaccat
     4741 tcacgttcaa aagattattt cttcagaaca attagacaac caggggcatg aaaaacaaga
     4801 aggagtaaat ggcgcgaagt tttctgttta tgatgtatcg gacattttac aaaagatgga
     4861 tgttaaggat ctcactactg accaaattga atcccaatta aaaaatcgag tgaaaaaact
     4921 atccagtgat caattgaagc tagtttctaa tgaagaaaca aagacaattg atcaacaaac
     4981 aggtgtcttt gaattctcgg tagaagtaca ggcaaatcag aaacaagctt attatattgt
     5041 caatgaatca agtcctgaaa acatttctaa tagcgaggat attttgctat taacacctgt
     5101 ttctgataaa aacggtgaat tcttaaagga tgtgtggatc tatcctaaat cagaagcttc
     5161 gcaacctaaa gaagaagtaa aaaaaatcgt ttctactggc gtaaagaaaa acttctttga
     5221 gaattgttgg gattttgtta ctcacttgtt ttctagagat taaggaagaa ataaaatgaa
     5281 actgatcaaa ttattattat tcaatttttc ttgtttaacc gcagtctttt tgtactgcgg
     5341 tttattattt tctaaactat ctattttgcc aagctaccaa ctatacctaa tgaggatttt
     5401 aaacatacca gctgcttcca tgccgctaga tattatggaa aagctcatta ggaatccatt
     5461 atttactacg atcaatttga tcgttattgt cggaatttca ctagtcattt ataaaaaaga
     5521 ggggatgaag ggatgaattt taaacactcg tggacaagac ggttaaaata tgtttttccc
     5581 ataatgatga tgttaggtac attgtttggg ttaaaaacag ccaatgtctt tgctgaaaaa
     5641 gttattgtcg atccgtcaaa tccaatccag aacgtaaaga ataattttat tattccaaaa
     5701 gatgttccta atgcaaaaac aaatattact gtaaacggag cgtcaaaagt ttacaaatat
     5761 catgttgatg ccaacaaagg tggttttaca gacgactacg taggattagt ggagggaaac
     5821 aaaaatatac gctatccttc tttccaaaaa gaatacggtg aaacaaacct tgttttagaa
     5881 ggcgtaacaa ccaacaccaa agtcaatatt gactatggaa aaatcggtac gtataacggg
     5941 aaaaaagtca atatcaagct agttctatct aatattcact tatactcgga tacgttgccg
     6001 tggaatctac tagataacaa ctatacaaaa actcattttc gagacgatgg ctataaaaat
     6061 acgaatggtg caatgagtaa atctaaaaaa agaacagtac tttggatctc agacaatctt
     6121 ttctcaggaa ttgtttacca ttcaacacaa atgaatgtgc agctagtagc cacatatgag
     6181 gacggtagcc ccgttcaatt ttcaggagat acatttatca gttttaactc tttgaaccct
     6241 gcaggaggaa aaagcacgga tcttaaagga gaatatgccc actatgacaa aatgaatacg
     6301 acagattggt atgtcagaaa agatacggtt ctttcagaat ttaaatcttt ctacaacaat
     6361 ctgaacgtgg taggtggcca cccaggagga agctctaagc tgacacaagc ggataatgat
     6421 ttcaataatt tgcatgataa attaggagat ccgaagtttg gtcaaggtac tgtgtccttt
     6481 aaaatatctg aagcaaatcc gacgtttgtg attggttctt ctaatgttca aacctggttt
     6541 actttatctt ctgcgacaat ctttagtgtt gttcctgatc aacctgaaaa aacaggggtt
     6601 gataaaaatg gaaataacgt gaacgataaa atgttgcaag taggagacac gattcaatat
     6661 cgaatcaaac agaaggtgaa tcgattagga gtggatttac tagcggttta cgatcgattt
     6721 gagttaatcg ataatttacc aaaagaagta aattacgtcg atgcacacgt tgaaagtggt
     6781 acaaataaaa aattcgatgt atctggtgaa gtgacctatg acaaaacaaa acaccaggta
     6841 aaatatgcag cgaaagctga tacattaaaa aatcgaatga agtataacgg cgaaacttac
     6901 gaattagtga tcaatgtaaa agtcaatgaa ttggccaatc aaaatagtgt agccaaaaat
     6961 cagggaacgt cgatcattaa taaagtagaa aaggatacaa atatcattac cgtttatttc
     7021 cctaaaattc cagtgaaaga agtacaacaa aatggaaaag atgtaaacgg acgaaatgac
     7081 ggtaagaagg gtacgccgac agctccttta aacgcaggat ctgaagtaca atatctagtc
     7141 acacaaaaat ggcacacaaa aggcgtagac gctgcgtcag atcactacaa acaatttagt
     7201 attcaagatc caattgaagc tcgtttgaca tataaagaag gttctgcgca agtaatagat
     7261 aagagtaaag gaaaagatat tacttctgaa ggcacactaa cctatgatag caattcaaga
     7321 acgctgaagt gggaagcttc cgctgacttc ttaagtaaca atcttttaga tggacgagaa
     7381 attcagctga tatttacagc taaaactcca ttacaatcag aaaaaaatat tgataaccaa
     7441 gccgtagtag cagtagataa tgtttctaat aaaacaaatg ttgtgacaat tggggttgat
     7501 cctaacttgc cacaggtcat tgttcctaaa acaggttcta cacatttagt aacgatttca
     7561 gcggtcagct tactattact tgtgttagca acttttagtt atgcggttct gagatacatt
     7621 taaaataaaa aaacgaaaga atgaataaaa tgaaaaagtt aataatagta gttacttcct
     7681 tgattgccat gattgttatt ttagtcggat gtagtaatga aaaaaataaa caaactaatc
     7741 aagataatgg cgtattgaaa tcaggaacta tgtggaaaga agaagtaggt ggtcttgttt
     7801 ataatttgaa aattatagac gagactacct gggaatactc tgagagtgta tggcatccag
     7861 atcctgtaca aataacagtc aagaggcaaa aagactataa aggtttagaa agatacaaaa
     7921 tagtagattc agctggtgtt agggaattta taaacaaatc tgattcttta tttattgttg
     7981 ttccctatga gaagaatggc gtaaaaaaaa taatttttct tggatctagc aaggatgaaa
     8041 agcgaacgaa agagaaattg attcacgatg gatcacaatc caataaatat aagttacaaa
     8101 agactagcga gtgattgcta gtctttttat ttgtcctgaa ggaggaatca cagtggcaaa
     8161 agctaaaacg ccatttgaaa aagccgcgtc taaaatctta gaacgccgcg gtgtatctga
     8221 aacacagtgg aaacaagaag tccttgaaaa agcacaattt gacctattac ggggaaaaga
     8281 caaggactta gaaggatata tctatcaacg tgaacgtgaa aagcttgttt tcgatgaaat
     8341 tattaaacac taaaaggagc gaacaatttt atgtttgaat caattacaat gaatttttta
     8401 acacaaccga aagtagtagc tgcggttgat atttttagag cattacaaac tgccgcaact
     8461 agtttaaaaa acaatctgat gatttttgct gctgctgcgg ccgttgcttc cattgttatt
     8521 gggggtcttt tcttcctatt tggacgaaga ggagctgaaa atgggaagat gcacgtaggc
     8581 tatgtattga tgggggtagg agttatttta gcagcagctt ctattgtggt ttcgttattt
     8641 ggattgtttg gacaaacacc tcaaggatta taagaaagta aaaaagtagg agtaagaagt
     8701 agtcgatact ttttactcct tttttatgaa aggagaatac tcatgtactt tacgattgca
     8761 ggcaatacca aaacagataa agagttaata aaaaaagcaa cgaaggaagt aggaaagcgt
     8821 acttcttttt taccgaaaac aattaaatat tctgtcattg aaacggaaga acagctacat
     8881 gtgttaggga aagtactgga acacgaaagt cgatcttctg ttaagttgac tctcacgtta
     8941 catgacacag aaggaaaaat agagaaagct ggtacagaat acgtttttgg cccactggat
     9001 tatcaaattt cagaaggttt ctatcctaca ttgatggatt taatccgcct tgatgtgttt
     9061 aaagaaacgg atgaaactat gagtgtggaa gagtcttatc gaaacgaaaa acttttacaa
     9121 gttttagaag aagagttaga gagcgaccaa agaaaagtga atcaagggag tgaagtcgaa
     9181 gtaaatgaag aagtaccatg ggaacgacca atatttccaa agaaggagtc aaaagaaaaa
     9241 gagacagtct atgttgacga acaagctgat attccgactc agaaatcgat atttgaggaa
     9301 gagaacgatg tgatgggtca attggaggta gatccctttg aagaaacaat ggaggatgaa
     9361 gagacggccg aagtagaagt tgatccagtt gaagcgaatg aagagtcaac atggactgaa
     9421 ggggattctc aggaatatat tgaaccactt atcagtgaag aagtagaaaa aaacgaaata
     9481 gagatacaaa aggcaactgt atttgattat attttggatc aatatccgcc agagcatgaa
     9541 tggatgaaag aaaaaaacaa attattttta aatgagcttt attcgaaata tcaattaccc
     9601 caaacctatc aagcattttt agagagtaaa gaagaattaa tctcacaggg taaaggcaca
     9661 cttttagaac agttagagtc tgtccaatca acaaattggc atgagcttgc acaagaagat
     9721 ttattagcca cctttgaaga acgtcaacaa gaaacagatc aagaaattaa tgagtatgcg
     9781 acggaacagt cgtctaattg ggagaaagca aaagaggaac taaataaaga agaacagcga
     9841 gtgattgaag aagaagtaca aaaaattaaa gagcgatacg aaaagaaacg cgacaatgcg
     9901 tattccttgt gtgtagcaaa gatcaataat tttactcaga cgaatcaaga agcgttatcg
     9961 aaagaaaaag aaaacttgtt aaaagaacgt attaaagaac gaaaagaaga attttaccga
    10021 ggtttgcgta cagataagct aaagattagc aaagaattaa atgaacaact gaataagcta
    10081 tatcaaacaa cgactgaagt tcttatggaa aaatacgaaa agatccagca agaattacaa
    10141 gtgcaaattc caaaatggaa aaaagagtat gaaattcaac aacaagaatt gaaagaacaa
    10201 gaagaaaaac aacggcaaaa agaaaaagag cgcgaacaat tagaactaaa aaaacgtgaa
    10261 caggatcttg cggaacaaca aatggcaata gaaaaacaac ggttaaacca agaaaaggaa
    10321 aaagaagcag ctgctcgtaa ggaacgtcaa gaacaaatgg atacattgcg acaagctcag
    10381 ttaaattatc tagcacaacc tcaaccaacg ccttcagtag ctgctgttca aaccccaatg
    10441 aacaaccaaa caaatctaac tccttcacca cagcgttcct ttaacggatg gatggtggga
    10501 tgtattgcaa gtttaagctt gcttctaggt ggaggaacag tatttgcttt taatcatatc
    10561 aatcaatctt cacaggctga ttctgtggcc acaacgacgt caatcaagga agaagcccaa
    10621 gaacaagcaa gaaaagagta tgaacaacaa ttagaagaat tattgaatcg taccaatcag
    10681 tcaacaacag agtcaagcat ggaaaatagc aaagagagtg attctagtaa ggctaaagaa
    10741 ggatcaagcg attcaactgt acaatcaaac acttccgaaa gttcagaatc aaatgaggtg
    10801 aaataatgcg tgttagagaa acaacgaagc gaaaagacaa acgattcaat ttagaaaaga
    10861 ataggtatct ttctcatttc tatcgctata ctccctcagg agaagagcta ccagaaaaac
    10921 atgacaaacg catttcgaaa aagatttttt atagtggact tgtatacctc cctttagagt
    10981 atttagtaac aagagtacca gaagttttca accgattgga aattgttaag ggaagcttac
    11041 aaggaataaa tctgtttcaa ttacaggaaa tgccacaggt ctttcatttt gattcagctg
    11101 gtttttggaa tccatttact cctacaacta gtttgggtac acgtttagca atttcgacag
    11161 tttgtgtggt tccagctgta cttatgggat tgaaaatgga catgtggttt cgtcctctag
    11221 cagatggaca aaaaggggat aatcgattaa tgaccttaaa agaaatccaa cagatttatc
    11281 cgcaaatacc tgaaacaaaa actacttttc caggttatgg aggtattcca atcacacatt
    11341 acaaaaaata ttggtacatt caaacagata ctgttaatac gtgtattgtt ggtacctctc
    11401 gatcagggaa aggtcagatt gaagtgttag ccaccattga caatttatct cgtgcagaaa
    11461 aacaaagttc aatggtcgtt aatgatccaa aaacagaatt atatgttggt agtaaagatg
    11521 aactagaagc tagagggtat gatgtatatg cctttaatat caccgatcca ctgcaatcaa
    11581 tgagtgataa tccattggca ctcatcgtga aatactggaa acgaggagac atagatactg
    11641 caacacagtt aaccaatact ttcacaaata cgatttatta tgatgccaac gcttcagata
    11701 acaaatactt taacgaaaac gcgcagaaag ctgtcaatgc gcttattttt gcgttgttag
    11761 aacaagctga tcaaacagga gattactcca aattgacacc gaataatctc gttgagcttc
    11821 tttctgagat tggcggtttt aattattatc cagatccaga caatgaattc aaacagttga
    11881 atgccttaga tgaatttatg aatcagttgc cacctggaaa cattgcaaaa aaacaatatg
    11941 gggcaacgaa aattggatct gataaagcga agggaaatat tttatcaacg gcaatcacgg
    12001 gattgaatcc atttactcta acgaaaattg caaagatgac ttctcaatca acgattgatt
    12061 ataaatcggt tgggtttcca aaatacttag atttgaaatt acatgaaagt cttttaaatc
    12121 aacgagtcgt agtagaattt tatcataagg gaaagaaatt tcatgaagaa cgtgtcaaag
    12181 taggctataa aggattttgt gaaatcaatt ttgattgtca tttagttgca ggtgatcaag
    12241 tttgtttaaa atacgatcat caaggaaaag aatatgtggc ttggtatgaa ttttctcaaa
    12301 gagtattgaa agacgaagag ggtaatatcg tttatcggaa aaaacgagga gaaacccatt
    12361 tgccagaatt agaaaaagaa gccgttctaa cattagatga ggagaaaagt aacttattag
    12421 tagagtctat tcggatgcac tataccgata aaccaatggc tatttttatg cttacacctg
    12481 attatgatcc atctaaccat atcattgtta gtatttttct tagtcagtta tataaagaat
    12541 tatctacgca atgtgttaag actaaaggag ataaatgtca ccgcaggatt cattttattt
    12601 tagatgaatt tgggaatatg ccaccaatgg ataatatgga aggaatcatg acggtcacag
    12661 ctggccgaaa catgttgtgg gatctgttta ttcaatctta tcagcaactt tatgcgaagt
    12721 atggagaaca aaacgcagcg attatcaaag aaaattgtca gacacatatt tatattatgt
    12781 cgacagatga taataccatc gaagaatttt ctaaaaaagt ggggaataag acggttgaac
    12841 aagagaacag taccatcaat tcattaggta tgaatactca tatcaatcgc aatgttgatt
    12901 cagatcgtat cttaactcct gaaagaattt ctactttttt agaaggtgaa acgattgtac
    12961 tcgctccttt aaaacgacaa gatttgaaag gtcataaaat acggccgtat cctattttta
    13021 atcatgggga aacgaatctt ccttatgctt atcaatatat tgaagatttc aatccatcgt
    13081 gggatatcaa tgatatagac atacattgta cacatgcgga cttggatctt aaggctttac
    13141 aaattgatta tgattatttc ctaccagatg aatatgctag aaatctctat catggactgc
    13201 atgatcaatt agaagaaaaa aacgaagaaa atatgtttta taaaatcgtt caatctcttg
    13261 agaatgagac ggctaaaaaa ttgttaattc aattttacga agaacaagca gcagatcgac
    13321 ttcaagaata tgttgaaaag ttgatcaaaa ataaagaaat ttctgaaatg attgggaaac
    13381 ggttgttatt ggaatctgaa aaggtggtga aagaatgatc attggaggaa ttttttctgg
    13441 catagccgac tggtggtcag gcttattcgg agaaggggat gcaaacccca aaaccgtaac
    13501 gaaagtctta caagattatg gtaattatct gcagtatcaa gatgtattta gttggatttg
    13561 gaacactttt ctgtggggat tgattaaagg attatataat ctcaatactt tactagaaaa
    13621 aaccatttat caaagctttg atttaaaaga cttattgaac gccgctgggg taaatgcact
    13681 atttcaaagt ttgattagta aggtattggc tcttttttgt gtcgtcacat taatttattt
    13741 aggcttgaaa ttttttatga gtaaacaccc accaaaagtc aaaaatatct tggttaattt
    13801 atttatggcc atgatcttga tttttggtgg gagttcttta atcgatgaag ggttaaatat
    13861 cagtaaaagc ttttacggag acgtcactgc agcaaataaa gcagataaaa cgggttcacc
    13921 tgcttttcaa attattcaaa ataatgtgat cgatgtcagt acattactag caactgaccc
    13981 gaataaagta gaatccctgc caacagacaa aagaaatgct ttaacagcga aaaattttaa
    14041 aacagcgaat atcaatggga ttattactcc taaacaagct aaagaaatgg cggataaaga
    14101 taagagtggt ttatcagatt tacaaaagga tcggttaaga gcgttacaat accgattgga
    14161 actagatgat aatgggaaag aaattcctgt tcaaattagt gacgaaggct tagcaaaata
    14221 tgtctatact tcagggtatc gtcgatacag tagtaaacca ggtattatct tagctggtga
    14281 atcttctttg gcggtggcct atttatttat cctatttacg atcgttactt gcattattga
    14341 gctggtattt aaaaaattct atttagtgat tgccgcgtca acggactttg agacaggcca
    14401 acggatgaaa agtgcgatag aagatatttc tcaatccttc ttgttattgg cgtttacgat
    14461 tttagaatta cgaatctata cattaatgct ttcaggtatc ggtgatttgc atgcggcagg
    14521 aaagatcaat ggattcttgt atgttgttgc tttaattgtg ctaactattg cgctattcaa
    14581 aggctctcaa tcggttacga agattttcgg tgttgatacg agtctgaaaa acggcagtaa
    14641 ttctcttctt agcatgtttg ccttaggaaa tattgcgaaa aatgtgggta gcggagctaa
    14701 aaaccttgca agtgctggta agagtggttt aagccattta aatgatattc gtaaaaatgg
    14761 atttggccat cgaaaagatg ttacccctga taacggggaa gaacaatcag acgataaagc
    14821 tacaactgta cctaatagta agaaaacagt ttccgaaatg ttcgggaaag cgaaaaatgg
    14881 tgtttctaaa gccgcagaag gtcttggtta cgtcaatgaa cgtgggttaa aaggaacagc
    14941 tgaaaagatc ttacagaaaa agccaaagac accttagaag agaaattaga cggcacgaga
    15001 gctaaagaat tggctgaagc tgtgaagaat ccatccgaag cactagaaaa acaaaaagaa
    15061 aaagcttcag aagtcaaaga aggactaaaa gatgcgtatg gtgaaggaca agttaatgca
    15121 gcagtaaaag ctaatacaac aaccgaaaaa gaaaatcaag aaggtatagt gattccttca
    15181 gtcaaagatc accaagaagc gttgcactct aatgaaaatt cgattgaaga gaaaaattcc
    15241 atagaagaaa gaaattcaga agaaggaaaa aatggacagt taccaaaaac tgatccagaa
    15301 caatatcgct ataaacctaa cagtgacctt gaaaatgaga agcgaatttc agatttatca
    15361 aaagtaaatc aagaagacaa agcagaaaat gagggacagt taccaaaaac tgatccagaa
    15421 caatatcggt atagatctgt taactccgaa aatgaaaaac gcatttcaga tttaccaaaa
    15481 cgaaatcaag aagtacaaac ggatgaggat tccatcgttc tgccaaatga taatggaatc
    15541 gaagttggaa ctgaaaaatc aacgaaaatt ggtttaccta aggcacaaaa tttaccaatg
    15601 aaagatagtc gtttgaatca attcaaggag aatgatcaaa gctttgttca ccaatcaact
    15661 gcaggtactg ttcaaatgaa accaagtcgt ttgcctgaaa atggaaatct gacaactgtt
    15721 tcttacaatt tatctgaaag tggtgagaca gggaatgcgt ccgttcaaaa tgttgtgaca
    15781 agagaagctg caccaaaacc tattcaaaac gttcatacag gttacaagcc agcaaatgtg
    15841 gcatttactg caccaacaaa aataaatgtg acaagagaaa ctaaaaacag gaacaatgca
    15901 ttgaaagaac aactattgag ggatatcaaa caggatatca aaccataaaa ggcctacaag
    15961 ttaggccttt tatttatatg aaaaggagaa atcaattatg gataaatata taaacatgaa
    16021 aaaccctcca agatttagtg gagaatggaa gtttttcgct tttttctttt tagatctatt
    16081 aacgcttggt ggctgctttt tttgggcgaa aatggtcaat aatcttgtcc acttaccact
    16141 cttttttgca ctcttgaatt atggcttatg gattgcgata gcgttagtga ttgtttggcg
    16201 accctataat aacccaggtc aacgacaaat ttttgcgatt atcgagtcta ttttttttgc
    16261 ggacaagaac cattatcatg cgttagatcc taaccgaaat tatcatggta cgtataagga
    16321 ggatgaataa atgcgagtca aaggacaaaa ttcagttgaa aggaaatcca ttttgcagtt
    16381 tggaaaacca gaagagcgct ctatcttgga cttatttccg tataaatcct gtacagaaga
    16441 gggagtctta attactagag aagatcattt tcaacgttat tatcgtgtag tttctacaga
    16501 cgtggaagga ttaaatgaac aagagaaact agaacgaatg aatcagttga ccaccgtcat
    16561 gcgtacgtat gtgccaacaa ttaagttagt tagtttgacc acagagacgg atctaacgga
    16621 acaaatcgtg caaaagcggc aattattaga taaaaatcgt atggaacagt ccttaggaag
    16681 aaatctacaa cgattaaaaa aatatgaaaa gaaattagtc gaagaaatcc aggaattgaa
    16741 acgagcagaa caagaacgtc ctgacttaag cttctttttc gtcattgaag gaaagacaat
    16801 caaggattta caaaccagaa accgtcagtt aatgcgatct agtggcattt tagggttaaa
    16861 accactctac aaaaaagaat tgatcaagat tctgtatcga atgaacaata tgaatgatga
    16921 gtaggaatgg agaagagaga atgaaatatc cgttgatatg gttagaaaaa gatgttccga
    16981 aggaagagac cactagaaaa tttcctcaca ctttaagcct gggtaagttg ggaaaagggc
    17041 aatcttttaa ccaagtaaag aatcaaacaa gaagaggtga ctgtacatgt tagaagcaat
    17101 taaaacagct ttttcaccac aaaatgatca aaaagtactt gatccattaa agcaactagg
    17161 aaatcgggaa atagaaattt taaaatccaa agggtatgat ttagattttt taaaacaagt
    17221 gatgccacaa ggtgggttaa attttgatga agaatatgtg acgtatggta atggttattc
    17281 acgatgtgtg acagtttatc actacacaaa aaatcctacg cttttttggt tggccaatct
    17341 catgaacaat cctcatacag tggccacact cgatgttagc acagatgaaa agcaaaaagt
    17401 gattgatcaa atcaatcgtg ccttagatga attaaatgat cgatcagata atgaacgaaa
    17461 agcgactgac cgaaataaaa gcgcagaaga atacctggaa ctagcggaat atgctcgtaa
    17521 attgacttct aatggggaaa tttctaagca agtcaaagtg agagtacatg tttacggaaa
    17581 cacgattcaa gaagtagatg aacgagcgca ggaactgatc gaaaatctaa aagggatgga
    17641 ttatcgtgcg gtgacttatc tgcataaaac caaacgagat tggcaatcga tgtttaccat
    17701 gttcgatgaa caagctgaat ggttcggaaa tatcaaaggg caatcactac catcgttaaa
    17761 cattggcgga ggtttcccgt tccaccacca aacgctaaaa gatccaggag ggacacaata
    17821 tggggaaacc atgacaggcg gcccatttgt ctggaatgcg acgtataaaa atagtgtacg
    17881 gttgtctact aataccttgc ttctaggaat gatgggagct ggtaaatcga ccgttcttaa
    17941 aatgatcgcg gaagcccatt tagctgcagg agactttatt tggggctttg aaaagggaaa
    18001 agactttatt cctttcttaa aggaatataa cggtattatg gtgcgtttag acggatctga
    18061 cggaatgatc aatcctttag agatatttgc cacacggacg tatgatgaag catccagttt
    18121 atatgacgat ggatcagtca aagatttgaa gatcaatgaa gcagcgtcct atcaaacaca
    18181 cttagacaaa gtggtctatc aggtacagtt agtgagtcct cagctaaaag gaacgatgaa
    18241 agcagaattt aagacctact taaatcgttt ttatgaagag tatggaacag taccacgcgg
    18301 ctttacttcc tcaaattcgc gttcaaatac ggagacgcaa gtcacaggta aagatccaga
    18361 agcttatccg acgttcaaag aatttttaga ctttttagga caactggaat taccaggtgc
    18421 ttcccaagag aaaaagaatc gaaaagaaga aatggaatcc atcgtagaat ccttatgtga
    18481 gacgtatggc atgatttttg atgggcatag tacgattcga caccttgacc aacaacagtt
    18541 ggtctttttt gacattgact ctattacagg cttagatcga ggggtacaac aatgtcaaat
    18601 gtacatggct atgacgttga tttggaatca ggcattgatt caaggaagaa gacaacgacg
    18661 tttaattaaa gaaaaacaaa tggaagaaca agacttcaaa cgcttcttgg cgatgattga
    18721 tgagtgccat aatgcgatta atccagattt tattgaaacg gtaaattatg tcacaaactt
    18781 tcaacgagaa atgcgtaaag tagaagcaat gactgtttta gctacacaat ccccacaaga
    18841 aatggtgcca gaaaatatgt cttctgataa cttctcgaaa ctgaagaaag tctttgaatt
    18901 ttcttctacg aagatcttta tgcgtatgga tgaatctatt ttacgtcata tagaaggatt
    18961 agtgggtaaa tcaatgacga actcagaatt agaatccatt cctcaacaaa atcaaggaga
    19021 cgctgtgatc aattttggct caaaagaaac cattcgtgtt cattttagtc cgaacaaacg
    19081 tcaattaaaa ttgtttgatg gaggaaaata atggaaaacc aaaatgaaag tttaatcaaa
    19141 cagtatgtta agcgtcgtgc aaagcggcgc ttatttttgt gggtgtttgg tacttctgca
    19201 ggtttgatta ctatattgat cactgtgttt gtgacccttt ttctaatact tgcggcaggt
    19261 tctattgata attctgattc aagtagtggt ggcgaagctt ttacaggaga atattcggaa
    19321 ggcttaccca tttataaaga aatcaaagga agaggccctt tttctgatga gattgcgcaa
    19381 tatgctgtag gtgcagcagt gaagtataaa ttattaccaa gtgtgatcct ttcgcaatac
    19441 ggttatgaat cagcgtttgg tacgtcggcg tccgcaagaa atgatctcaa ttattttggg
    19501 attacgtggt ttgatgggtg tctgtttcct aaaggaactg cgcgtggaat tggtggcatt
    19561 gaaggtggct ggtacatgaa gtttcccaac tcaaaagctg ccttctccta ttacggcttt
    19621 atggtagcca cacaatcaaa ttttaatgct tgtgtcggaa ataaaagccc tggcgcaagc
    19681 ttattgatct taggacgagg agggtatgcc gccgcaggaa ttactgaaga cagtgcctat
    19741 tataaaaatt gtatgtctat tattaataca aataaattga tggagtacga tgagtttgcg
    19801 attaaacact ggagagaagg cggcggaact ggtggtacga ttacaggaag ctggggcaat
    19861 ccattcccag gaagtagctt agataaaaat tcgttcagtg gaggacaact ttttgggaaa
    19921 aatccaggag gagaattccg acctaacggg ttccatgatg gactagactt tggttcagtc
    19981 gatcatccag gaagtgagat tcatgcggtt catggcggaa aagtcgtgta cgttggcaat
    20041 cctggtattt caggtttggg tgcgtgtgtg atcgtgatta attacgatgg cttgaatatg
    20101 gtgtatcaag aatttgcaaa tagtacagga aattctcgag tgaaagtagg cgatcaagtg
    20161 aaagttggtc aagtaattgg gatacgagat acagcacact tgcatttagg atttactcga
    20221 atggattggc gtcaagcaca aggacatgca ttcattgatg atggtacatg gatcgatccg
    20281 ttaccatttt tgaatagcag caaaaaatag atcaggagaa aaaatatgga aaataaaacc
    20341 ttaaaaatcg tcttaacagc tgcaattagc gttagtttaa tcggcaatat tgcgttgttt
    20401 tggcagctta aagaagaaca agagacaaat acctataaaa ttgaagaagt ggctaaaaac
    20461 gcgtctgata agctcaaaaa aaattcttct gaggcacagg cgaagatcga caagttaaat
    20521 caaaccatca atgagaaaaa ccaagaaatc gatcagctga agcaacaaga tggcacaaaa
    20581 gcaaaagagg aagtaactga agcacaaaga aagacggcag aagagttcgg tcaattggcc
    20641 attgataaat cattaagcag aaatgaatta acggaaaaac taaaagatgt cgcaacccaa
    20701 gaagtgatcg acaaattaag tcctgcagac gatgatcacc aacatgacga ctacggaagt
    20761 tactctttta cattaggtga cgtgcaaact tacgctcaaa caagcagtgt aaaagaggaa
    20821 caaaattttg tcgtatttgt tgattataca atcggcaatc ctcaatttaa agatgtcaaa
    20881 ccacaaaaaa taaaaggtgg attaactgtc actgaaaaac aagtagatgg tcaatggaaa
    20941 gtcacagact ttagttattt cactcgttag gaggaagaaa atgagtaaga aaaaaggagc
    21001 aagacaacgt tttaaaaaac gaaaaagtta tcttagatgg ctggggatct ttatgattct
    21061 cggctttttg attcttgcta gtccgattta cacacataaa aaggtagaca taccagatga
    21121 agtaagcacg aacgcatttt taaacacgaa ggaactgacg atggtttcaa aagaaaaagg
    21181 agcgaaccag caattagttt ttgaatttgc gatcgacgat gaagatcaga gcttattaaa
    21241 agaactagct aatcttaatt atcgtgtaga agtaaagaca gctaaagggg attatcaagc
    21301 aataaaagcc aaagtgatca aggtgtctga tgattatttt gtagtgaagt tagcacatgt
    21361 accagaagaa tatatcgcca tgcgtttaac cattattcct gaaaaaattg atccaaaagt
    21421 ggatatgcaa gaaccacaag atctgattta ttacgttcac gaagataagg ttaaagatca
    21481 agtgaaagat gaagattatg aacaacatgc gatcaattac aaagtgaaag gatacaaaaa
    21541 agaacaaaaa gcagcacaaa aacaaatgga atccttccaa gcaaccattg atttgaatga
    21601 agaattagtg aagaagctaa aagatcaatt gccttatcaa gtcgcagatg atcaggaaaa
    21661 tactgaaaac aagattaata gctaccagca agaaattgaa acatcgaaaa cgcaaatgaa
    21721 agatcaagaa gagatcattc aaaaacttca agaaaaaatt gatcgtatga caaaagaaaa
    21781 gatttagaag gacaggtgga gtgaaaaaat gaagcgaaca aacttggctg acaaagatac
    21841 tttcgctgag cagaaagtat tgattaaagc ctatctcaaa gttgtaaaag ctaaagaaat
    21901 atgtcgagaa gtgaatttat ctaatacaac aatatctgag ttgaagaatg gtcgaaagaa
    21961 tttagatact ttaaaattca gatcatttga agctctatat gtagctgcta aaaaatattt
    22021 tacagctgat aacaaataaa aaacaggagt attttatgtg aaaaaagtat ataaaaaatg
    22081 cttatgaatc agctatatca ccttaaccat ttgttaatga gatgggagaa aacattaacc
    22141 acgattctaa cgcaaacatc aagcacataa aacatttaac actcctcctt tctggctgaa
    22201 gatttcaacc aaacaggaag acagcgtatc aattatagca tacacttttt cttaaattca
    22261 tttttcatat gacaaaatat ttccttctaa tcgacaaagt tagaaggatt ttttattgtc
    22321 taaatacaca aataacctta atttataaag agcataagga aacgtgggaa cactttttaa
    22381 aaaaagtgtt ccaataggct gtgcctatta cgtttctcaa aaataattgt gggaacaaaa
    22441 tatttaaaaa taggatagtg aaaggatagc aaaggatagt aaaaggatag aagcaggata
    22501 gcgaaaagga taggctcctt ttcgctatcc tttttaaatt ggaggaatta atatggaagt
    22561 aagaataaga aatgtagatg agaaaatcgt aagagaaatt gatcgaagat gtgacgaact
    22621 aagtaaccga acgggtaata aatggagtcg taacgattat ttaaagctac tcgttgaaaa
    22681 tgattttgat cgtcctttga tggaatataa acaagataaa tttgatcaat tattaaatca
    22741 gttaatagat aaattttctt ttgttcaaag tcataataca aaagtattag aagaatatat
    22801 ttgtcagaat aatcaaatta tcgaactttt aattgaacaa agtagaggag gagaagttta
    22861 acatggccaa agttatagac aagcatattt atttttatga aacacatata gatgttctag
    22921 atgaaattat tgcaactcat gcagaggtta aaaactatag tgaagccgtt cggtttctat
    22981 gtatgaatta tgattcagaa gtggagaaaa aagtaaattc caatcaagca aagttgaatg
    23041 caatgggaaa agaaatttct attttaaccg aaatggtttc agagtttgcg gatctccatt
    23101 tgaaagatac agggatttta attggtacaa attcaactgt ttaccaagaa gcaaaagagc
    23161 gtgtggaagg caagataaaa gcaaatcaaa cgaagaaata ttctcctaag ataaagaaaa
    23221 caataagcga atcagtgact acaaaaagtg aagtatttga tccaacaagg ttcttaaaac
    23281 gataaaaatt agaaggagaa tgtgatgagt aaatcacctg cagtcattct cacttgtcaa
    23341 tttaccttgc ccaataaaaa atctttttca acatacattg attatatgac aagagaaaaa
    23401 gcgctggaag aaaaggaacg acgtacgccc gaggaagaac aggaactgcg tctaatcaag
    23461 aatgctttag aggattttta tattcctgaa ggagaaactt tttcggagag taagacgggt
    23521 aaagaattca gcgaaacgtc gaatgaagcg caatcgttga tgaaacatgg aatcaatttt
    23581 gacaagtcag aagaacaaga ttttaccaaa tatctttctt atatgacacg tcactatgcg
    23641 ttagaacaaa agaaagtact gacgcatagt gaacaaaaag aataccaacg cttgaaccaa
    23701 gcaataaaaa aatatcaaac agagccacac aaaaaagctt caggaaatct tcctggagtt
    23761 ttttctatgg cttcagacga agtgaaagga agtgatctga aggagatcaa gcaaaccttc
    23821 caaagaggac aacaaaaagg ctcaatcatt tatcaagatg ttgtctctca tgatcattcg
    23881 tatcttgaaa aaataggatt gtacgatccc aaaacagata ctttagatga agaaggttta
    23941 aaagcagctg gaagaaaaat gatggagaca ttgttcgaga aagagcaact gaatgaaaca
    24001 ggctactgga tggcaacgat tcaccgaaat accaaacata tccatattca ttttgcagtt
    24061 gttgaaaaaa aaaatacgcg taaaccatac actgagattg aaaatggtgt gagttatatc
    24121 gtaccgaaag ggaagagaag gcaagcaaca ttagacgaaa tgaagactaa ttatgtacat
    24181 gagttagaac gtttttctta tgaaaaaaat cgaatgggta tggagaaacg aaatttattg
    24241 acacggaaaa gtgatttgcg taacacctta accaaagtag tgaaagagcc agtacgttat
    24301 gatcgacacg caatgagctt attaactgaa gtatataaaa acttacctaa aaagcgttcg
    24361 gaatggcatt acggggatga aaatcgaacc aaactttcac catacacaag agagaaactc
    24421 gatgagttaa cccaatatgt gttaacaaaa gaacctggat atcaagaata tatacagatt
    24481 tcaaaagcat tgaaagaaga agcaaaaagt ctttatgggg aaagcaagag agaatcaaag
    24541 gatgcagaaa aaaatgctct ctttgattta aaaaagcgca caggaaatgc cattctaaca
    24601 gaactgaaga aacaggatag tcaaataaaa cctttaatag aaaggatgga tcagatgcaa
    24661 tctatttaca atgaacgatc ctttcgaaca cttaaacagt tgccaacgaa tgaatacaaa
    24721 tatagttacg aagaattcat gaagcgcagg aaacaattcc aacaaagagt tgtcacgcaa
    24781 cggagtttgc gtcaattaaa tcgaacgatt aatgcccatg aagaaaaata ccacgcaagt
    24841 aaagcatacg aagagcaaca acgaagaatt caaatggaac aagaaagaca gtaggaggaa
    24901 tagcaatgaa acaatattta gatcaatgga aagttataga aggatcatta agagaagaac
    24961 gaatcgaaca acttcccgat tgtttagaaa aagagcattt gtttcagata agagaaatgt
    25021 tgataaacga acagtttgat ccaaatcaat ttttagtcgt tgagtaccca gctactggtg
    25081 tgtactgttg taatcatgtg aagggtgaaa aatattttat cattcaagaa tatgagggca
    25141 agttagcgcc ttattatact acttgggaaa tgaatgaaga ggggatcaat aacttcccgt
    25201 gtaaaagcat tgaagagtct atttcactca cagaatgtta gttgttaagt gaataaaaga
    25261 agtaaaagag tcctgaacgt gattgtccag gactcttttt tttcactgaa ttgttttccg
    25321 ccaattcatt gagtggtgcg atttccgtct acattatatc aaaagaaagg aggaagaaga
    25381 aatggcttat tcaacgattg gagttgttgt tgaaaaaagc agagacaatc ttgtttttgt
    25441 gacagaaatt caaacaggca gagcttttgt agtcacagac aaagcggcaa aagcatatca
    25501 aagtggcgat attttaacgt taaatctgac tacgaagacg ttcgtcgatg cggcagaaga
    25561 ttatccatt
//
LOCUS       DACCXO010000031        25531 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.032, whole genome shotgun sequence.
ACCESSION   DACCXO010000031 DACCXO010000000
VERSION     DACCXO010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.032"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            130..2190
                     /locus_tag="IVY70_001190"
     CDS             130..2190
                     /locus_tag="IVY70_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958327.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2273..2842
                     /locus_tag="IVY70_001191"
     CDS             2273..2842
                     /locus_tag="IVY70_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7958328.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKSDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3017..3823)
                     /locus_tag="IVY70_001192"
     CDS             complement(3017..3823)
                     /locus_tag="IVY70_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958329.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3961..5475
                     /locus_tag="IVY70_001193"
     CDS             3961..5475
                     /locus_tag="IVY70_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP7958330.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5878..6861)
                     /locus_tag="IVY70_001194"
     CDS             complement(5878..6861)
                     /locus_tag="IVY70_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP7958331.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6866..7513)
                     /locus_tag="IVY70_001195"
     CDS             complement(6866..7513)
                     /locus_tag="IVY70_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7958332.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7598..8230)
                     /locus_tag="IVY70_001196"
     CDS             complement(7598..8230)
                     /locus_tag="IVY70_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7958333.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8246..8797)
                     /gene="coaC"
                     /locus_tag="IVY70_001197"
     CDS             complement(8246..8797)
                     /gene="coaC"
                     /locus_tag="IVY70_001197"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP7958334.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8794..9531)
                     /locus_tag="IVY70_001198"
     CDS             complement(8794..9531)
                     /locus_tag="IVY70_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP7958335.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9528..10517)
                     /locus_tag="IVY70_001199"
     CDS             complement(9528..10517)
                     /locus_tag="IVY70_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP7958336.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10775..11317
                     /locus_tag="IVY70_001200"
     CDS             10775..11317
                     /locus_tag="IVY70_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7958337.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11334..12419
                     /locus_tag="IVY70_001201"
     CDS             11334..12419
                     /locus_tag="IVY70_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7958338.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12431..13237
                     /locus_tag="IVY70_001202"
     CDS             12431..13237
                     /locus_tag="IVY70_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7958339.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13238..14065
                     /locus_tag="IVY70_001203"
     CDS             13238..14065
                     /locus_tag="IVY70_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7958340.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14062..15135
                     /locus_tag="IVY70_001204"
     CDS             14062..15135
                     /locus_tag="IVY70_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7958341.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15563..18259
                     /locus_tag="IVY70_001205"
     CDS             15563..18259
                     /locus_tag="IVY70_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7958342.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18336..18824
                     /locus_tag="IVY70_001206"
     CDS             18336..18824
                     /locus_tag="IVY70_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7958343.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18836..19330
                     /locus_tag="IVY70_001207"
     CDS             18836..19330
                     /locus_tag="IVY70_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7958344.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19351..20160
                     /locus_tag="IVY70_001208"
     CDS             19351..20160
                     /locus_tag="IVY70_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7958345.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20157..20978
                     /locus_tag="IVY70_001209"
     CDS             20157..20978
                     /locus_tag="IVY70_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7958346.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21029..23128
                     /locus_tag="IVY70_001210"
     CDS             21029..23128
                     /locus_tag="IVY70_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP7958347.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23125..24243
                     /locus_tag="IVY70_001211"
     CDS             23125..24243
                     /locus_tag="IVY70_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP7958348.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24282..25160
                     /locus_tag="IVY70_001212"
     CDS             24282..25160
                     /locus_tag="IVY70_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7958349.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25306..25503
                     /locus_tag="IVY70_001213"
     CDS             25306..25503
                     /locus_tag="IVY70_001213"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958350.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         8067 a         4447 c         5407 g         7610 t
ORIGIN      
        1 aaacaataaa aaatcaaaaa aaatactctc ctaatagtcc atcttctttt gattttattt
       61 atataatgaa atcatcttat gaaaattact acttcatgtt tagacaagag tagggaggac
      121 taagaataaa tgtgttcaaa aagacgttca aaacaactaa ataaacacgc ttgtcgttta
      181 gataaggaac ggtcgtgttc tgaaaaacac tacgttaagt ttatgatgcg cacgatgctg
      241 atcagtttat ctgtaggtgt ctatatgcat gtacccgttc aggcaacgac acaaaccatc
      301 aaggtcgtac gtacctctgt ttctgagacc actgattccc ctatcactat tactcatact
      361 ttcactgatg caaatggaaa acagctactt cctgcattga cttcctataa taaagtcagt
      421 tatcctccaa cgataaacca ctactggtta attcctaaca aaacattatg gacatcggaa
      481 accggaagca gtgtgccgct ttctatcttt tccgatatga ctgatcctac aaaaggtgat
      541 cagttaaaac agatcactga tattttgaat catgctttga cagatataaa ggacggtgga
      601 agcgcacaag tttcttatgt atatgaaaaa gattattcac agtttgaaag aaagaaaatc
      661 gatgttctgg cagggacaaa agtttcgata tctgatctgg tagaaaggct gaagaacgca
      721 gatggaacac aaggagatat gtccaaagta atttcaaaca atgggaatat cgtggatacg
      781 actaatacag gaacatctac tatttccttg tcctattttg atccaattgc tttaaaaaca
      841 atgactgcat ctgctgtgat ccatgttcgt gctgatcaaa cagctatcaa aggaaaagat
      901 cttcacttaa gaacaggaga gagttacgcc cctaaagatc ttgttgaata tgtaacaaaa
      961 agcgatggaa cttcaggaaa taaagaagaa atcaaaatga acgggacagt agatactaca
     1021 actccgggag aatatcccgt tgtattaact tatatagact cgaaaacatc aaatgtggca
     1081 gaaacaacag ctgtagttta tgtagaagca tctgctgctt catcgactgt catcactcat
     1141 tttatagata gtcaaacaaa taaagaactt caagcagaaa cacatatgag tagtgtaaat
     1201 cgtattcctg atttgcgcct acctgctgaa ttttctgatc gagtgccaaa tatagattta
     1261 tcgacttatg aacaaaatgg gaaagtcact tctatttctg attggatgca agcaaacgga
     1321 ttagacagaa aaagtcactg gtcggagatc cttgaagtac ttcactcaga actgatgaat
     1381 aataggaagc aatcaggagg ggaaattacc tttacttatg tttatatacc agacaatcgt
     1441 tcattagaag gtcatgatct tattgttcat ccaaacgaaa aaataaacaa agaagagttg
     1501 atccaatcag caacaaattc cttaggtaaa cccgtaccgt taaaggatgt atcggttaca
     1561 atcaatgaag taccattgga ggatggttat ttgaccaaac aaacaggtga ttttacgatt
     1621 atgtatagtt atcatgaccc gtattcactc caagaactcc atgctcagtc actattacat
     1681 gttttaccat taaacaaaca gatagatcaa aaagaacaaa aaagacaaga agaaagcttg
     1741 tcagaaaaaa aacaacaaat ggctcatgca gtttctccaa cagggaaagt aaaacaaaca
     1801 agaagcctag agttagcaag aatatctaga aaaatctcag aatctaataa aacgataagg
     1861 caagaagctc atttggcccc acaagtttct ggattggaaa atgaaatttt agagcctaaa
     1921 ataaaacaac aatcagaaaa agaatttgtt gctaaacagt cggaagatca gaaggaatca
     1981 gatgaccaga aggaaacgaa gaaaaaacgc ttgtcaacta ttgattccac cgatgagcat
     2041 ctgcagccgc aagtaccact tcctagtccg ggaacacccg cacctggtgg aggtattcct
     2101 tcttctgctt taggagattt catgcgaggt atggcaggaa catgggtata cgctaatcga
     2161 aatgaatttg acgcagatga ctggttctga tttatatctc tatctgttat agtcaaataa
     2221 gtagctgagt catatgttat ttggcataat ggagacgagg agggatagaa aaatgttaga
     2281 taaaacgtta ccttacgcag aaatctggat gacaagacca cttcaacaaa atgttccaat
     2341 cacgccttta gcggaaggtt ttgaatttcg attttatcaa aaagaagatg aatctgcttg
     2401 ggcggagatt gagacttccg ttggggaatt tcaaacgaca gatgaagcac ttgagtattt
     2461 tcaacaaaac tttgctcctt atcctgaaga acttgaaaga aggatgtttt ttgtagagac
     2521 aaaaaaagga gaaaaagtag caacctgtac cgcttggcga aaaaaatcaa gagagcaagt
     2581 gtatccggtt tttcattggt tagcagtgaa atcagattac caaggaaaag ggctcgcaaa
     2641 agcattgact gccagagtct tgcaagaatt tcctaccctc catacggacg gaccaatcta
     2701 tcttcacaca cagacatgga gccatcaggc gattgctctt tataaaaaaa tgggttttac
     2761 ttttattgct gaaaatctag acggcacacc taatccagat tatgaaaaag ttatgcaggt
     2821 tttaagtaaa ttagataaat aacgaaatgg tgaaataagt cctcagtatt gggataaaaa
     2881 acgaaatagt cattcctcat atcttacttt tttgagttaa tactatcgga taactaatgg
     2941 catgccatac attcaaaaaa gaaggggcag tgacaaaagt attgtcactg ccccttctct
     3001 tgttttttag catgttttat cgtttgattt ttttaggaat aaaattcttg atgaacgtaa
     3061 atttttggta atcgtttcga ctatagaggt aaccaaatac actgaaaaca actgctccaa
     3121 taaaattaac aaataaatcc ttcatagtat ccatgattcc cacgtctaag taaccatttt
     3181 ggatgattgt tttatctgtt ttaccatctg cattttgact ttcaatgatt gtccgagtga
     3241 tatctggaac atgatagaca gtgtttccgt ctttacttaa ctccacacta ttgatttgct
     3301 ggatgatcgt atctttttgc atatcgaatt gcagtaattt atctgctgaa aattccacaa
     3361 actcccacat aacaccaact gtcatcgaaa aacagaagga aacaactgct agtaataaag
     3421 gagtcaagtt catggttttt gtatttttat tgagcaaata aacaagggaa aagccgacac
     3481 ccccagctaa aaatccgctg aatgcatgta gaaaagtatc ccacattgaa aaatagccat
     3541 aaaaatcact taactctcct aaaactgttg aactaaaaat aaaagataac aaaatgattt
     3601 ctagcatatt tggaatctgt atctttaata tcttttccag aaaataaggg atttcaaata
     3661 aaattaatgt cagtaataaa agcattgtat ttccccagtg ttggttccag acctgatcca
     3721 ctagcataat aacgacgatg atacgtaata aaatatagat tccgtttgtg attctacctg
     3781 cagtttttgt actgttcgta aaaaaggttt tcaaatgatt cataaaataa ctccttcatg
     3841 tatatcctta attatactaa gaagcgtgtg tttaccaaag ctatatgact taaagaaaaa
     3901 cagagttgat attgtgaaaa aaatcactta atatagtata aggagaaaag ggggatacaa
     3961 atgaaaaaat tagttgctgg tttattaaca gccgttgcac ttcttgctat gagcgcatgc
     4021 ggcgcagatc agaaaaatac agcgcagaca agttctagta cgaaggaatc tgccgaagta
     4081 tcatcaggcg cttctgaaca agtatataca gatccaaaag aaatgaaaga atcttatgat
     4141 gtcgtgatta ttggagctgg tggtgcaggg atggcagcag ctatcgaagc aaaagatgct
     4201 gggttgaatc cagtaatttt ggaaaagatg cctgttgccg gaggaaacac atctaaatca
     4261 tcaagcggta tgaatgcttc agaaacaaaa tttcaaaaag aagaaggaat caaagactcg
     4321 aatgatgcct tctatgaaga aacgctaaaa ggtggtggag gaacgaatga taaagaaatg
     4381 cttcgatact ttgtcgatca ctctgctgat gcggtcgatt ggctagatca aaacggtatc
     4441 aaactggaca acttaacgat cactggaggg atgagtgaaa agaggacaca tcgtccgtct
     4501 gatggctcgg ctattggtgg atacttagtg gaggggttat tgaataacgt ccatgagcgg
     4561 gaaatcccta tttttgtcaa cgcagatgtg acagacatta agaaaaatgg ggaaacggtc
     4621 aatgaagtga ctgtacatgt tcaaggagaa gaaccaaaag aggtcaccgg aaaagcaatc
     4681 gtagttacca caggcggttt tggtgccagc aaagaattta ttgaaaaata tcgtcccgat
     4741 ttagctgatt atattacgac aaatcaagaa ggatcaactg gagatggtat caccatgatt
     4801 gaaaaattag gtggacaaac cgttgatatg gataaaatac aaattcatcc aactgtgcag
     4861 caggatgagg gggtcttgat tggtgaggtt gttcgcggag aaggagcaat cttggtcgat
     4921 caagagggga aaagatttgt caatgaaatg gatacaaggg ataaagtatc tgcagcaatt
     4981 acagcattgc ctgaaaaatc agcttatttg atttttgacc aaggcgtgcg tgatcgtgcc
     5041 acagctattg aattttatga ccaaaaagga tatgtcacaa ctggaaagac aatggaagaa
     5101 ttagcagaaa aaatcgattt accaaaagaa acactagcaa aaacagttga tggatggaat
     5161 acggatgttg cggagaaaaa agatgctcaa ttcaaccgtg ccacagccat ggaacatgac
     5221 ttatcgtctc cgaattatta tgcgatcaaa atcgcaccag gaatccatta cacgatgggc
     5281 ggtgtgaaaa tcaatacgaa cactgaagta ttagataaag aaaaccagcc aattaggggg
     5341 ttatatgctg ctggtgaagt cacaggtggc ttacatggaa acaaccggat cggtggaaat
     5401 tcagttgcgg agatcattat ttttgggcga caagcgggca tacaagcagc tcgatttgct
     5461 tcggaaaatg aatgaaatag aacgtaaata aaaagaggtt gtgaaatcag cgttttgagc
     5521 taataccgag ggtcagctga ttgaacactg tttagtgaag aggttgtgaa aaagctgccc
     5581 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggttgg
     5641 acttattacc gcagatgcaa gcttttgaac accgtttatt cggaaaaaga gcgtgggaca
     5701 aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga agcagaagaa
     5761 attccttcgg aaataagctg aaattcacaa aaatttgaaa agcaattttc gtgaatttct
     5821 tcttatttct tggagttaaa cgcttctgtc ccagcctctt ttttgatcag catgatttta
     5881 ttcagttatc ttcaaaatga tttttccgat gtttttgttt gcttccatat attcatgcgc
     5941 ttgtttgact gcgctgaatt ggaatgtacg gtcaatgatt ggccgaatct tgccattttc
     6001 tagaaaaggc ccagcttttt gcataaaagt ttgtgcgagc tctgctttat actgatcact
     6061 tctaggtgtc aataatgtac caagcagttg gacacgtttc ccaatcaata caccaaggtc
     6121 tatctcaggc acgatggtcc cacctagcat accaatcaag acccaacgtc catcaacggc
     6181 tatagaagct aaattctttt cccagtaaga agcgccgatg aaatccaaaa taacatcgac
     6241 tccccgacct tcagtgattt ttcgtacctc ttcttgaaag tcttgttttt tatagttgat
     6301 caattcatct gcaccaagtt ctttacaaaa ggcgagtttt tcttctgatc ctgcagtaac
     6361 gatgattttc gcgtgagaaa gctgtctagc taattggatc gctgctgttc ccacaccact
     6421 tgctcctgca tggatcaaga cagtttcgcc ttcttgtaat tttccaagcc agtaaagtgt
     6481 ttgataagca gtcaaaaaaa cttctgagat tccagcagca gaaacatagt ctagcgtatc
     6541 agggagaagg attgcacgat cggcaggcat cacagcatat tcggcataac cacctagatt
     6601 gactaatccg tatacttttg tcccaggagt aaaagtggag atatcacttt tattctcaac
     6661 tacctctcca gctacttcga ctcccaaaat agggtaggga ggtttcaaag agactgattc
     6721 tcttttcata atatctgttc gattgacagc tgctgtatgg actttgatca gtaattcgcc
     6781 ttgttttggt ttaggaacag gcacttcaat ttgttctaat acatctacag aaccaggatg
     6841 cttgattgaa atggcacgca tggatctatt cctcctttgc ccattcttgg aatgctgtat
     6901 tcaactcttt gctaccttca gaaagtacta gagtcgtacg agaaatatcc gtttcttcca
     6961 ctagagcggc aagtacagca gcaactgtag agcgagtgat cgtttttgat gccttctcat
     7021 taggcttagt taattgaatg gaagtaactt cttcatcgtt agtcaatgtg actggttgaa
     7081 gaatgacaaa atctaagtta gttcgtttca gccattcatc tgcataatgt ttagtaatat
     7141 aataatctgt catggactca ggccattttg ctggttcatc tgcataaaca gcactgacca
     7201 tcagatagcg agagacatgc gcattttcag ctgcgatcac tgttttgatt gcgccatcca
     7261 agtctacttg cagaagattc tttccttgag agccggcagc aaaaatcaat acatcgatgc
     7321 ctttgaatgc ttcggtcatt ttttccacgg accaagttaa atcaaaagag acatacgaaa
     7381 cattctcttt ttttactaat gtttgggaag caacatctct cacccctgca aaaatttcat
     7441 gggaacttgg agctaaatct tttatcaggt gtcggccaat ttgtccgttt gcacctacaa
     7501 caaaaatttt catttggatc atcctttcga cgtcaaatct ttccattcaa tagtaacacc
     7561 aaaagagaaa aaaatcagag atcggctacg atgactatca gttagtaaga gaagggtgct
     7621 tgaagtatct taacaagaca ttgcatactg ctgcagtcgc aagacctgcg acgattcctt
     7681 caggtatacc acttgtaaaa ataaccgcta gaatagctga ataaacagca tttaaatttg
     7741 ttccgatgac tttggcataa tcttgctgga ataaaaagta gatcatattc atcactaaaa
     7801 tcgtattggt caatgaaccg gaaagtccag ctaagaataa agaaacggaa tctgcttttt
     7861 gtttcattag tttgcgcaga cctttataaa cgaaataagg aaccacccca atcaagatgc
     7921 gaggaataaa cgcgatcagc aaggctttcc agcttccatg ttctgttccg atgactggga
     7981 taaacggaga aaagacaaaa gacaaaggtg tttggataac tgtgctacgg atcatgctga
     8041 tcagtccaaa tgtaccgcca agaaaagcac ctagtcttgg tcctaaaacg atcgaagcaa
     8101 tgattacagg aatatgcatg gttgttgcat tgattggacc gatagggata aaacctaaag
     8161 gcgtaacagc taataaaatc aaaatagcta aaaacatcgc agttaatgta aagttttttg
     8221 tatttttcat aagtactcct tttttctaag catttctttc attaagagtc agcatgacct
     8281 gttcgatgat cgtctgaata tcagctaagg caccttttcc aaaatctcca caagctaaaa
     8341 gagattctct tggttcaatt tcctgatagc cgacagatct caatgtttcc agattacgct
     8401 gagtgatcgg gttttgatac atattcgtgt tcatggcagg ggcaatcaat tttggtgttc
     8461 ccactggaag cgccattgcg actgttgaaa gaagatcgtc tgcaatgccg tttgctagct
     8521 tgccgataat gtttgctgta gcaggcgcga tgagaaacaa gtcagcttgt tttgctagtt
     8581 cgatatgatt gattacctgc ggatttgatt cttgcatgac gtctgtatgg actgcgcgct
     8641 tcgacagaga ttgcaaggtt aatggcgtga taaaatgtgt gctgctttta gacataatca
     8701 catctacctg ggcacctaat ttgacgaatt gacttgtaag atcagcgctt ttgtaagcag
     8761 aaatacttcc agtaacgcct agtaagatct ttttcatttt gattcctcca gtctaatttt
     8821 ctcagtaatc aatgcagcaa tctcagattt actttgagct tcttccactg ttccatcttt
     8881 agataagaga tagccgtggt gtgtctcgcc atggatttcc gtcaaatcat ttgcgaaaat
     8941 aaaatcagtt ttgttattta tcagactatt ttttgcgact gccagaagag attcctttgg
     9001 tacatctact aatagtttga aaccaatgat ttttgctttt ggctgccatt cacgtagata
     9061 agaaatgatt tttggtgttt tcttcaaaac gagcacaaga tgctcggtgt tagaagagat
     9121 ctttgaaaca gtggtatctg tctgttcatt ttccgcaaac caattgtcca gttcttcttg
     9181 cgttgaagaa gtgggtaggt tttttgccag ctgttcttct gaaaaactaa aagcaggagt
     9241 aaaatcactg actgccatac tgtggataat cgcatcgtag tgttcttttt tcgttagttc
     9301 ttctaattgt aaaaataagt ccagtgcaga ttcgatcgta taaatcgtta tattggagtg
     9361 tcttttcggt ttcagtgctt gtttcgtagt cacgtaatcg attgtggtat ccggattggc
     9421 agctaaataa tcagctaatg cttgccctaa tcgtcctgtc gaatgatttg taatagcacg
     9481 tacttgatcg attttttcag tcgttcctcc agctgtgatt agaatcttca tttatggtca
     9541 cctacattta ctagtgttct tccttggtgt gttccagcaa gtagtttaga tgctgtttca
     9601 ggcaattgat ccagagtaat ctcttggacg accagttcat ctgcaatctg caaatccgcc
     9661 atctgttgcc ataaagaaag acgttgatct attggtacat tgacagaatc aattccaatc
     9721 aactggatgt ttcgcagaat aaaaggcaag acagtcgcgt ttagtttgat tcccccagca
     9781 ttgccacata aagctactgc accgttataa gagatcaatg gaagaagact gcttagctgc
     9841 tcgccaccta ctgtgtcgat gacatagtcg atttgctgtt ttcctaaagc ttttgttgtc
     9901 tctggcagaa attcatcagg tcgtacgact tgcaaagcac ctagactttt tagccatgtg
     9961 acagactctt cttttctgga aaaggcaatg attgactgga atcctagttt atgtaacagg
    10021 gctatcgctg tactgcctac accgccagaa gcaccagtaa cgacaacttt tttatcggag
    10081 gtcatacctt gacggattaa agcgttcaca cacaacatag cagtaaaccc tgctgtacca
    10141 aggatcatcg cttgacgcag cgtcatattt tttggtagag gaactaacca gtctcctggt
    10201 actttttgat attggctata accgcctgga tgggtgacgc caagcccaaa accagtagca
    10261 agtacaagat cttcttttga aaaacgtgga ttgttacttt ggacgacagt tccggataaa
    10321 tcaatccctg gagtcatcgg atatgttcgt atcacctgac cagactcgct cattgccaat
    10381 gcatctttat aattgacatc agaataggca atttttacca agacttcatt ttcagccaac
    10441 tcagtaaatg gcagatcact gattgctgta tggaaaacag gttcctttat tacctgaaaa
    10501 gatcgaaaag cgttcattcg tgagacactc cttttaaaat tttcttttat cataccataa
    10561 agcctactct tttattcttg ttcttccgta aatttagaca aaaattaggt gaaaaataaa
    10621 gaatcaaaca actagaattt attccaaaag ataaagaaat attcagcaaa aaaaatttta
    10681 caaagggttg cctttttcaa taaatttcgt atactaatac gagtggtagt aaacaggaag
    10741 tttactatta gaaaacttcg attcggagga aattatggaa attggtgaga agttaagaaa
    10801 tttgcgcatc caaaagaatt tgacacaaga agaactaggc gaacgcacag atttgtcaaa
    10861 agggtatatt tcccagttgg aacgagattt aagttcacca tcaatggaaa cattcttttc
    10921 tattcttgaa gtcttaggtg tgactccaga agaatttttt catcaagaag cagcgaatct
    10981 gcaagtggtt tattcaaaag aagatcatac ggtttatttc gatgaagaaa acgggtatga
    11041 attggaatgg ctcgtggctg attcgaatga aaaagaaatg gagcctgtct tgctgacttt
    11101 tgaaggtgca ggagaatata agacgtttga accttcatta tctgaaacgt ttatttttgt
    11161 attagaaggg gaattagagt taagcttagg agagacggtc tatgtggcaa aagcaggaca
    11221 gtcgatctat tatcaagcga caaaacaaca tcaattaagg aatcatctca aaaaacgaac
    11281 aaaagtattg attgttgcaa cgcaatcata tttgtaatat agggggtaaa aacgtgggga
    11341 aaccaatcat ttcatttgat caagtagtga aacgctatga tgatgaaaca gtattaaaaa
    11401 aagtaagttt tgagatcgaa caaggaaaat tttatacact gcttggtcct tccggctgtg
    11461 gtaaaacaac gatcctacgt attatagctg gattcaccga agctacagaa ggggacatct
    11521 attttgaagg gaaacggttg aatgatttgc cagctaataa gcgacaagta aatacagtct
    11581 tccaagatta tgcacttttt cctcatatga atgtttttga taacgttgct tttggattga
    11641 agatcaagaa aatgtccaaa tcagatatcg aaaagaaggt aaaagatgct ttgcgtatgg
    11701 tccagcttcc tggatacgaa aaacgggaga ttagtgaaat gtctggtggt caaagacagc
    11761 gtgtagcgat tgcacgagca attgtaaatg aaccgaaagt attgctatta gatgaaccgt
    11821 tatcggcgct tgatttgaaa ctacgtacag atatgcagta tgaactgcgg gatctgcaac
    11881 aacgattagg aatcacgttt atttttgtta ctcatgacca agaagaagcg ttggcaatga
    11941 gtgatgagat ttttgtcatg aataaaggga aaatcgtcca aagtggtaca cccgtagata
    12001 tttatgatga gccaatcaat catttcgttg ctgattttgt tggggagagc aatatcgtgg
    12061 acggtgtaat gatcgaagat aatctagttg agtttgttgg taagcaattt gaatgtgtcg
    12121 atggcgggat gcgtctaaat gaaccagttg aagttgtttt acgcccagag gatttgacga
    12181 tcacgacacc agataaagga aaactagtcg taacggtcga tactcagctg tttcgtggtg
    12241 tccattatga aatcatctgt tatgatgaac agcaaaatga atggatggtc cattcaacga
    12301 aaaaagctaa agaaggaagc aaagtaggat tggcctttga gccagaagat atccatgtca
    12361 tgcgtttcaa cgagtctgaa gaagaattcg atgcacgtct tgacagctac gaagaatagg
    12421 ggggacaaga atgaaaacaa tgcgcaggat ctattccgtc ccttatattt tgtggctcgg
    12481 tttattcgtg atcgcgcctg tattaatgat catttatcaa tctttttttg atatgaatgg
    12541 gcaattcacg ctgaataatt atctatctta ttttacgtcg gggacatcgc tttctatgac
    12601 gatcaattct gtatggtatg catttttgat cacgtttttc acgctgatca tcagttaccc
    12661 gactgcttat tttttaacaa aactgaagca caagcaatta tggctgatgc tgattatttt
    12721 gcctacatgg gtgaatctgc tgcttaaagc ttacgcattt attgggattt tcagtgtgca
    12781 cggcagcgtg aatcatttct ttgattttat cgggatcggg ccgaaacaga tattattcac
    12841 agacttcagt tttctgttcg tggcaacgta tatcgagatt ccatttatga ttatgccaat
    12901 tttcaatgca ttggaagaga tgaatccttc tttgatcagt gctagtagag atttaggcgc
    12961 gaatagcttt gagaccttcc gacgagtgat ttttccgtta tcattaaatg gtgtgaaaag
    13021 tggtgtccaa gcagtattta ttccttctct ttctttattc atgctgactc gtctgattgg
    13081 cggaaatcgg gtgatcactt taggaacggc tatcgaacag catttcttgg ttacacaaaa
    13141 ctgggggatg ggttctacaa tcggtgtgat tttaatcatt gctatgttca tcgtgatgct
    13201 cctgacaggc gagaagaaaa aaggaggtcg ctcataaatg aaaaaattta aatggtcgta
    13261 tctttattta gggatcgtct ttttattctt atatgcaccg atcttctatc tgatttttta
    13321 ttccttcaat gaaggaggaa cgatgaacag ctttacagga tttacttggg aaaattatga
    13381 agctgttttt gaagatacac gtttgatcat tatcgtattg aatacgttca tgctggcttt
    13441 cttatctgca ttgatcgcca cgatcatcgg gacatttggg gctatgggaa tctattacac
    13501 gaaaaaacga cgtgcacgga cagcgttgtt gagcatgaat aacatcttat tagtctctcc
    13561 tgatgttatt atcggtgcta gtttcttgat tttctttaca ctactaggat tcggattagg
    13621 ttttcagagt gtattgcttt cccatattgc tttcagcatt ccaatcgtcg tcttgatggt
    13681 cttgccaaaa ttgcaggaaa tgaacgattc aatggtcgat gcggctagag accttggtgc
    13741 taataatttc caagtagtca aaaatatcat tttgccttat ctttctccag gaatcatagc
    13801 tggatatttt atggcattta cctattctct tgatgatttt gcggttacat ttttcgtcac
    13861 aggaaatggc tttacgacgc tctcagtaga gatctattct cgtgcaagac aaggaatcag
    13921 tttagaaatc aatgcgctaa gtgcgttagt tttcttgttc tccatgatat tggtcattgg
    13981 atactacttt attagtaaag aaaaccgtcc aaaacggctg aagaaaaaca gaagagtacg
    14041 cgagggggtg gcacggctgc gatgaaaaaa ttacaatctc tgtttttagg tattctcatc
    14101 attattgcgg tgctgctgct tggtgttcgt caacttgaaa aaacaagtgg catgagtgga
    14161 gcaaaggtac ttaccatcta taactgggga gattatatcg atcctagttt gatcacgaaa
    14221 tttgaaaaag aatatggata caaagtgaat tatgaaacat ttgattctaa tgaagcgatg
    14281 ttcacaaaaa tccagcaagg tgggacaaac tacgacatcg ctataccaag tgaatatatg
    14341 atccaaaaaa tgatcaaaga aaaactagta cttccattag atcattcgaa gatcaaagga
    14401 ttaaataata ttgatccacg atttttagac ttggattttg atccagataa tcagtattcg
    14461 attccatatt tttggggaac tttagggatc gtatataacg ataaattctt tgatgcaaat
    14521 caaattaagc attgggatga tttgtggaag ccggaattaa aagacagttt gatgctgatt
    14581 gacggtgcac gagaagttat ggggctttct ttgaatagtt taggctattc tttgaatagt
    14641 aagaatatgg cacagctgac agaagcagca aataaattga atcatttaac accaaatgtc
    14701 aaagcaatcg tggcagatga gatcaaaatg tatatgatca atgaagaagc ctccgtggca
    14761 gtgacatttt ctggcgaagc tgccgatatg atgtgggaaa atgaacattt acattatgtg
    14821 atcccaccag aaggatccaa tctttggttt gataatatcg ttatgccaaa aaccgtgaaa
    14881 aataaagaag gtgcctatga tttcatcaac tttatgctag aaccggaaaa tgcggcacaa
    14941 aatgcagagt atattggtta ttctacacca aatgaagaag caaaagcgtt attgccaaaa
    15001 gacatttcgt cggacgaaca attctatcca tctgatgaca cgatctccca tcttgaagtc
    15061 tacaaagatc taggatcgaa atacctaggc atctataatg acctattctt agaattcaag
    15121 atgtatagga gataatggca aaactcagta agaatgggga cttatcaacg ttctaaagag
    15181 ctaaaaatcg tcctttttct atctgagttt tccaatcttc taccaaaagc ggatgaaacg
    15241 ccgctttttt tgtgttattt tattttttct accacgtttt tctaccagag aaaagtggac
    15301 aaaaccattg taaaataggg cccgagacgg gctagtaaat ctaagattga aaaagttaca
    15361 gaaggataaa gtaattggaa actatgcaaa tatacttaac ccttctgtaa tctattaata
    15421 aaactaatat atgaatccag ttcaatattt gtagctatga aaaaacataa aaatcatatt
    15481 agataaaaac taaattggta gataaattat tttgtcagat atctgaggca aatattcgac
    15541 tatttgtgtt tcaaagtaaa aattggaata caaatataga atagacatat tgcagccgat
    15601 agtttattat gcttttagac acattaaact ggaggaaaaa atgaaaaaga gcgctaaaga
    15661 tcgagttttt gatgagctga tacgactaac agggaatatg tcttctgtca ctgccgagaa
    15721 aatttcgaag aatctaggta tctctcgtca gaatgccagc cattatttga cgagattagt
    15781 ggaagataaa aaggtcgaga aattaccggg aaagcctgtt ttgtggaagc ctttagatga
    15841 atatgcggtt gtagataata cggagcaaat aaacgaagcc tttcattctg tagttggtca
    15901 tgacggaagt ctgagagagg tcattcaaaa atgtatagcc gctgtcaaat atccgcctaa
    15961 cggactcagt gtattaataa atggagccac gggtgttgga aaaagttttt tagccacaaa
    16021 aatttttgaa tacgctatcc atgagcaaat tattgaaaaa gatgcaccat ttgctatctt
    16081 aaactgtgcc gattatgcgg acaatcccga actgctttcg gcaacactgt ttggatacaa
    16141 gaagggggcc tttacggggg ctgaaaagga taccgatggt ttattggcaa cggccaacaa
    16201 cggctatctt tttctcgatg aggtccatcg cctatcgaaa gaaaatcaag aaaagctctt
    16261 tctatttatt gatactggaa actatcgtcc agtcggtgaa aatgtaaatt ggcattctgc
    16321 taaggttcgt tttattttcg ccacgacgga aaaaggtgaa aattatttat tagacacctt
    16381 tgatcggcgt attcaaatta gtgttatgtt gccaactttt gaagatcgcc ccattcgaga
    16441 acgattagag ctgattcaat tattttttca aaatgaagcg gatgttttac aaaaagatat
    16501 tattgtttca agagaagcct tgtctttaat gttatctcat ccctttagtg ggaatattgg
    16561 taagctgaag aatatcatta aaatcagttg tgcggatgtg tatagtcgaa cacaagaaga
    16621 accattaacg attgggaaaa atgagatctt gattcagtta aatatgctgg aatccgaagt
    16681 agaaagcttg ccgattgcta gtttattgat aaagaaaaat caaaagataa aaacggatcg
    16741 aatcaaaccg tttgattatg attcgatttt ggaaaaaatt actcagctga taaatgaagg
    16801 taatttcaat gaaaattttt cttcgataaa gaatgaagta cagcatttgg tgcatcacat
    16861 tgataaggat tcttttttat caatgagtag aaaattgatg attcagatgt ttagaaaggt
    16921 ctgggtatcc gttttaggaa aaacgtacgg attgacgacg agtggacctg ttgcagaaat
    16981 tattagcaga tcctatgtca tttccccaag ctataagaca gacttaaccg ggataaatcg
    17041 tacattagct gcaaatctac ctcggacgac ttacttatgt aatcagttct tggagcattt
    17101 accaccgctc tcagaaaagg acgagaactt tctaagactg cttattgtcg tgactttatc
    17161 tgattatgta gatgaatcca tcgagctgaa gggcctgttg cttgctcacg gagatagtac
    17221 ggctagcagc attcaagcag tagtgaatca attatgcggc aattatgttt ttgaagcgtt
    17281 ggatatgcca gtagagagta atgtgtcaga gattgtcgaa tcgacaagga atttcgtgaa
    17341 acggcaagtg aatacagata gtctagtgct gatcgtagat atgggctctt taaatcaaat
    17401 ttacacacag atcaagaacg atttaaaagg ggagttactt cttctgaata atttgacgac
    17461 ttccattgca cttgatattg gcttgaagat gacgatgaat gcgccattta aacagattgc
    17521 ggaggatgca gcagacaagt attcaatcaa tgttcaatac tttgaaggct tttctcaaag
    17581 tagcaacatt atcatttcct gtatgtcggg tttggggatt tcggacaaat tgaaagatgt
    17641 ttttcgtcat catttatcgg atgaatccat cgaagttttc acgaaggact acagagaact
    17701 acgtgaatta atcgaccaaa atgatgaaac ttattttgac aagacaaaat tggtcatcac
    17761 gacttcggat cttccacgtt ccttttcaat tcctaatatt aatgtttatg acatcttaga
    17821 cggtgatgga gcagccaatt tggagcatat tttgagtcct gaaatgaagc cggcagaatt
    17881 taaccgcttg attcaggagc tagtaaaatt cttttcgttg gaaggaattg cggaccgttt
    17941 gaactttctg aacccaacga tcgtgatgag tgaagtggaa acagtcattt ctaaatatga
    18001 aaattactat catttcactt taaatggccg tgtaaaacta aatctctata tgcatacggc
    18061 attgatgatg gaacgtttat tcttggctcg gacagaaaaa tctgaagaac cgaattatga
    18121 tttgaaacca gatgaagatg aattttatta tgtttctaga agtatttttc aatcaatgga
    18181 gatgaaatat aattttaaag tcaatgctta tgagctttcg ctgctctatg aattattagg
    18241 accgtatatt caaaaataaa gtgtctaaaa aagtgtctaa tctgtttttt ttaggttaga
    18301 cacttttttt aatgtgaatt aacgtgatta aaccaatgtt tttaaaagtt ggcacaggaa
    18361 ttgcttatat aaaagtgaac agaaagaaag gaattaacca tatgaagaaa attctaatag
    18421 cgactcacgg tcacttagct tcaggctttc ttagcagtat tcaattactg acggggaagg
    18481 ttcaagagat tacggtgatt aatgcttatg tggatgagtg tgattttgaa caggagttga
    18541 atgtatttgt aaaccaggtg aacgagcagg atcagatttt tgtttttacc gatttatttg
    18601 gcggaagtgt caatcagaag attaccaaaa cctttttgga gaaagagatt gctgtaacta
    18661 ttttgtctgg ctttaatctg ccaattctgc tcgaaatagt attggcaacc aaggaattaa
    18721 caaaggagga tattcaggaa ctggtaaata aatgccaaca ggaattaaag atgaccattc
    18781 ttgatgaggt acatgtagta aatgaagagg aagatttttt ttaggaggaa gaaaaatgat
    18841 taaagcagtg cgaattgatg aacgattaat tcatggacaa gtagcgatga cttggacaaa
    18901 gagtctaaat ttaaccgggt tagtagtagc aagcgatgag gcggctacca acgatattca
    18961 aaagatgaca ttgaagatgg ctgcaccatc gaatgtcaaa gtcattatta aaacagttga
    19021 tgacgcgatt catttgttaa aagatccgcg ggcgaaggac atgcgcttga tggttttagt
    19081 ccctaatgtc aaagatgcgg taaaagttgc gaaagctttc ccaacggaaa tcgaattaat
    19141 gaacgtgggg aatgctggga aaatgacacc gccatcggaa gataagaaaa ccttatcaaa
    19201 ggaagtgatg ttaacgacgt cagaaattca agacttaaaa gaattagttg aactttatcc
    19261 agataccgtt tttcagccta caccggcgat ggaaaaacgt tcagctatct ctattttgag
    19321 tgatttttag aaaaaggaag gagaaaaatt atgtatgatg catttgtagt cgctttggcc
    19381 gtttttattg gagttgctgg acatgaaatg ttcgggatgt ccatgctgag tcgtccgatt
    19441 gttgttgctc cgttagcagg agcgttgatg ggggatttgc agacggggtt gattgtcggg
    19501 gcgtcactag aagccatctt tatgggtgtc gtcaatatcg gaatctctag tacggcggag
    19561 ccttctttgg ctgctgggct ggctacagcg ttcgcaatca aaatgggagg aaacgtggat
    19621 gcgattattc ccattgtctt tccactagca gtattaggat tacagttaat gaatatgatt
    19681 ttttcttttg tatgtggacc gatggctgct aaatttttca aatttgcaaa atctggtgat
    19741 caaaaaggaa tcatcaatct gcacttcttt atgtggtttg tccactatgc gctttatgct
    19801 cttattccat tctttgccgt tttatttgga gcagatgttg tcaaaagcgt cttggataat
    19861 attccagagg tcatcatgaa cggattaaca gtcgctggaa atctgctgcc ggcagttggg
    19921 atggcgatgc tgttacggat gctttggagt aaaaatattg ctgtgtggtt tttctttggg
    19981 gccgttgtta tggcgtatct cgagttacca ttgattgcaa ttgccgcagt tggaacgatt
    20041 attgcaattg ttatagccca aagagatcta caactaaaaa atatatctgt tcatgcagtt
    20101 caaagcgatg ctgctgtaaa tgcaacgctt gaccaagaag aggaggattt ctttcaatga
    20161 aattagctga tggaatttct aaagatgaga aaaaattggt tcggaagatg ttttggcgct
    20221 cgatgactat gtatgcttcg gtgaatccgg tgacgatggg cggcggtggt ttcgcctatt
    20281 ctatgatgcc cttcattaat catttctaca aaagtgaaga agatcgagct gcggcgctgg
    20341 aacgtcatac acagtatttt tcgacgacca ttccttgcgc gtcctttgtc atggggattg
    20401 cggcatcgat ggagaaggaa aattctgaaa aagctggttt tgacgtggaa agtatcaacg
    20461 cgatcaaaac gagtttgatg ggaccattgg ctggaattgg cgattcattg ttctggggag
    20521 tttggcgtgt cgttaccgcg ggactagcga tcaacttagc caatgccgga aatattttgg
    20581 caccaattat ctttttagtt atgttcaata tacctaatta cttgatgcga tactatggcg
    20641 gcgttttagg ctattcatta ggctctaaat atattgaaaa gctgtatgcg aacgggttaa
    20701 ttgaaattct gacaaaagct gctagtatct tgggtctgat catggtaggt gcaatgacga
    20761 tcagtatggt caaattcaat accgttttga gcttttcaat gaagggcgaa gaactaatga
    20821 atgtccaaga aatcctcgat agtattttta aaggaattgt accgttattg gtaacattga
    20881 gctgtttcaa attactagat aaaaaagtcg gcgtcatcaa aattattttt ggaatggtcg
    20941 tcttgggaat tttattgtca ttacttggaa tcgcatagta ggaagctgca gctactaggc
    21001 tgcagttttt ttagcaagga gggaaaagat gactattaat gaagtaattt attcacccaa
    21061 acacccattt tttaatgctg agttaaacga cggaaatgtg actacttcaa acgaatacag
    21121 ctactatcgg aatattcctg atgtaaaggg attgaccttc gatgtaagta atcaaaagac
    21181 attagcaaac cttgatgttt gcgggacgat tcaaacgttg accttttatc gagaaaattt
    21241 actaactgag gaaaaaccag gtgtttgggt caataaacag ctctctcaaa ccaattcgtt
    21301 atccttaaag gttgaagtaa acgggattgt ctaccatttg tcagaatcgg atcatcgtat
    21361 tgaagtcgat gtgattcaag actgtttgcc tagatatatt catcattatt cgacgtttaa
    21421 ggtcattgta gtatcctttg ctcccatact ggctaataag cgtttaagta tgcttgttca
    21481 acaggtttac attgtgaatg aaacacacga aagccttcaa gtttcagtga aagatgtgcc
    21541 ttcgtatcaa gagaagtatt cggatcagca aaatgtgttg atttctcaaa aaggaaataa
    21601 ggacacgatc gcacaacagg aagtggcaag cttcgccatt gcttttgtgg acccaaatgc
    21661 ttttgaagaa gtaatgactt ttcaggaatc tgatatcgaa aaatggctga aggaaacatt
    21721 gaactatttt gctcagctat ttgggcagct gtcaatgcca aacgatagag ttgtccatct
    21781 ctatcgtcgt gcactttatc aaagcttttc tagtttcggt atgaatcgac agggacaggt
    21841 cgtcggcagc aattggggca gctatcctgc tactaatcga atttggaata aggatatgta
    21901 ttacgcatca ttgccattca ccatgtttga accggagtta tgtcaaaaaa cggttctatg
    21961 gtttgatcaa tatggcgtta agtttcctgg ttcaaaattt acaggaggta tcaatcattc
    22021 tctgagtaat tcgttatcca gtgcgttact ggctagtctt tattttgaac atactgggga
    22081 tacaaaattc tttgagcggt gtccgcaggt tctactgaat gcttcaagaa ttattgagga
    22141 catattagct caacgtcggt caggggaacc aatgcttttt ccatctgttt ggttatcaga
    22201 cgcctttgca cttggaaagt atcataccgg cagcaatctt tgtctttgga aggcctgtat
    22261 tggacttgca gaaatttttg aagccctaga acaactgagt cttagcaagc gatacaaaaa
    22321 cattgcctgt aaactaaagg aatcaatttg taaacaaatg actatcgatg gtccttttgg
    22381 tgagcaattt ttggagggaa tcggtgatga ggagaaaaca acctattcag tgaagcacta
    22441 tcaaaaaccg attcttgagc aagggttaat ctttttaagt gatgtgattg tggatggaaa
    22501 aattaattta ttgatgcatg atggagaaga atcagacact acgttgatgc ctttttatca
    22561 atttttagat aataaagatc atttatatca gaacaccatg gccttttctg ctagttcttt
    22621 caatccaacc tatagcgagg aaatcaaagg catcacttgg ggattagaat ccggcgccac
    22681 ttttcctggc tttatcactg ttttaatgag cgagctgcac aaccctcaag ctttcgtaac
    22741 ccgattagaa gaactaattt gtttggcaga tttagacggt tcgtggtggt ggtggccgta
    22801 tcgcttagag gctcaacaag gagaagtcgt gcgagacttt ggctgcggca aatgcggctg
    22861 ggcatcgggt ctttttgtca gcctaatgat cagtcaatat ttcggattga aattcaaaaa
    22921 aggagtattg caaattgatc ctgttgataa tttaacgttt tcttggaaga atttgaagtt
    22981 cggtcaagcc ttcatttcga ttgaatgtac gcaatccgag ttgagtattt caaatttgtc
    23041 agaaaagcca ttgaaaataa gtgatacgaa aaaaattctt cacgtagaaa agggaaaaac
    23101 gattcacatt caaaggagaa aaagatgagt atactaaaaa atattaccca atttaaaaaa
    23161 atccagagtc gagcaatcac tcccgaaaat ccaacaggcg agaaaggtaa agctgcgatg
    23221 ttgccaagcg agctaggacc aagccgcaaa ggtcgtggcg caattccgct gacttctggt
    23281 gaagaaactg tgattgcgga tattactgga acgggaataa ttcgtcatat gtggatgacg
    23341 attcgtgatc gaacagaatt tggcagtttt gttttacgtg atttaatctt aagaatttat
    23401 tgggatgggt cggatagccc tgctgttgaa agtcctttag gggatttctt ttgtaatggg
    23461 tttggagagc gttgcgaggt gaactcgttg ccgatagtgg ttaatcctac agggggcttt
    23521 aattcctatt tcgaaatgcc tttccaaaca tccgctaaaa ttacgatcga gaatttacat
    23581 ccgaaggata tgtcctcatt tttttatacg ataaattacg ctttggtgga cgaattggaa
    23641 gaaaatagcc tatattttca cgcaaattgg catcgggaac gcattaccaa attacaggag
    23701 gattataagt taattgatcg attaaagggt caaggctatt acgtgggtac ttaccttgcg
    23761 cttacctgcc tagaacgtta ttggtggggc gaaggcgagt ttaaattcta cattgatggg
    23821 gatagtgagt atccgaccgt aacctcaaca ggttcagaag attattttgg cggggcgtgg
    23881 gcctttcatg agtacgatga gttcggacgt ccacatgcga aaaactattc aacagccttt
    23941 ttagggtatc cttaccaatc aaatagagat gccacaagag atcgttttga aacgggaaaa
    24001 ttgaatgccg ttcatgcctt tggtgacgac gcaatgccac gacatggtct ttatcgttgg
    24061 catcttcaag accccatctg ttttaccgaa gatttatcgg tcactttaca acaaatcggc
    24121 aacgatgatg ttcgcttatt tgagcggcaa gacgatgttg ctagtgtcgc ttactggtat
    24181 tcaacagaag cttcaggaaa tactaaacgt catttcactg atcgagaacg cttgccgcgt
    24241 taatgaagtg tttttcacat attcgaaggg aggaacctat gatgatatat ggggcaattg
    24301 aagctggagg gactaaattt gtctgtgcag tcggtactga ggaattaacg attttggaaa
    24361 gagtcagttt tcccacaaca actccagttg aaacaatgaa gcaggtcatt gattttttta
    24421 aaccttttaa ggaacaattg gcaggcatcg gggtcggctg ctttggtcca attgatattc
    24481 aatgcaaatc aacgagtttc ggtcatatca catcaacacc aaaacttgct tggcaaaatt
    24541 tcaattttgt aggaacgcta aagcaatatt tcaatattcc gattgaatgg acaacagatg
    24601 tcaatgcggc ttgctatggt gaatatgttg ctggttctgg caaagggtta gcaggtgttg
    24661 catacttcac tgtgggaaca ggtatcggag gtggtgcctt gttcaatggg actttcgtgg
    24721 aagggttcag tcatccggaa atggggcata tgttgatcaa gcgacatcca aaggacaatt
    24781 tttcaggaaa ctgtcccttc caccaagatt gtttagaggg gatggcagca ggaccagcga
    24841 ttgaaaaacg gttgggtgta aaaggccaga atttattggc tgacgattcg ttttggcaga
    24901 ttgaagcctt ttaccttgcc caatgtgcat acaatacaac actgatgttt tcgccagata
    24961 gaattatttt tggcggtggt gtaatgaaac aagaacacat gaagaaaaaa gttcaggata
    25021 aatttgttga gcttataaat ggatatgttg aaataccacc aattgatagc tacattatta
    25081 cgcccgaatt aggtgataat gccggaataa tcggcggatt agcattggcc agaaaagcgg
    25141 taagaaataa gcaaccataa ttaatgaatg tttcattgat actcatgtca aaagtattta
    25201 accgagtaat caaatgatca aatgaagttg gataaagcga aaatgaattc aaataaaaag
    25261 gctctaagtc aaatcgtgtt gttttcatta aatcactgaa aggaaatgct tctgcgtttc
    25321 cttttttctt tttgttgtgc gctgtatacc ttgaggatat tcactggtgg cgtacactta
    25381 aatagtaata tattgtgtta ctttatgtct gtggtaatag ttatagttgc ttaccaatgt
    25441 atgttcattt ttcgaaatgt tgacttcata atccttggct taatgtttga atttttttgg
    25501 tgatagattt tttcagccgt ataaaataaa t
//
LOCUS       DACCXO010000032        24609 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.033, whole genome shotgun sequence.
ACCESSION   DACCXO010000032 DACCXO010000000
VERSION     DACCXO010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.033"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(152..550)
                     /locus_tag="IVY70_001214"
     CDS             complement(152..550)
                     /locus_tag="IVY70_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP7958351.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="IVY70_001215"
     CDS             complement(547..1320)
                     /locus_tag="IVY70_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP7958352.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="IVY70_001216"
     CDS             complement(1317..2000)
                     /locus_tag="IVY70_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP7958353.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="IVY70_001217"
     CDS             complement(2167..3054)
                     /locus_tag="IVY70_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7958354.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="IVY70_001218"
     CDS             complement(3051..3920)
                     /locus_tag="IVY70_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7958355.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="IVY70_001219"
     CDS             complement(4057..5343)
                     /locus_tag="IVY70_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7958356.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="IVY70_001220"
     CDS             5616..6194
                     /locus_tag="IVY70_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7958357.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="IVY70_001221"
     CDS             6194..7123
                     /locus_tag="IVY70_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7958358.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="IVY70_001222"
     CDS             7120..8856
                     /locus_tag="IVY70_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7958359.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="IVY70_001223"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="IVY70_001223"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP7958360.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="IVY70_001224"
     CDS             complement(9752..10261)
                     /locus_tag="IVY70_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP7958361.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="IVY70_001225"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="IVY70_001225"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP7958362.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="IVY70_001226"
     CDS             complement(11562..12653)
                     /locus_tag="IVY70_001226"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP7958363.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="IVY70_001227"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="IVY70_001227"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP7958364.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="IVY70_001228"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="IVY70_001228"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP7958365.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="IVY70_001229"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="IVY70_001229"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP7958366.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16616..17590)
                     /locus_tag="IVY70_001230"
     CDS             complement(16616..17590)
                     /locus_tag="IVY70_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP7958367.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17571..19454)
                     /locus_tag="IVY70_001231"
     CDS             complement(17571..19454)
                     /locus_tag="IVY70_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP7958368.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19472..21697)
                     /locus_tag="IVY70_001232"
     CDS             complement(19472..21697)
                     /locus_tag="IVY70_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP7958369.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21812..22846
                     /locus_tag="IVY70_001233"
     CDS             21812..22846
                     /locus_tag="IVY70_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7958370.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22913..23926)
                     /locus_tag="IVY70_001234"
     CDS             complement(22913..23926)
                     /locus_tag="IVY70_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7958371.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24004..24192)
                     /locus_tag="IVY70_001235"
     CDS             complement(24004..24192)
                     /locus_tag="IVY70_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP7958372.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7075 a         5346 c         4401 g         7787 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tgcaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccga tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aagaattgta cctagtatag acataaaaaa atcgactgtc aatcattttt atgaaacttt
    16441 ttttaatttt ctgatagtta actttgtttt tcagaaaaat aacaattggt tttattgaag
    16501 aaaatagtca tgaacaaaag tatctgagaa cttttttact ctcttgttca tgacatatag
    16561 aataaagcag ctttgtcttg taaactccgt ttttcaaata ataatctaaa gattgctact
    16621 gaataataaa gccgatttca tggtcctctt cgctgctgat tcggtaaggt gactcaacat
    16681 ccgctcccca gctattgata cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg
    16741 ttcgtctttc tggcggtagt tcttctatat gtgttgcctg ttccagttct tctgctttga
    16801 atggtaaagc ggaaaaagca aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt
    16861 cttgatcttt atccgtatta tccaacgttt gatttcttgt tagttctacc catttcgttt
    16921 ccatatgcat gccgcattct tggggaacaa ggtatgggga aacggggagt cctgccactt
    16981 tataaatacc ttcgacacca ccagccatcc gatccggata tgtttcccct gataggcctt
    17041 catactcaaa aaattttgct ttcgttggca taatgaacct taaacccaat gcgggtaatt
    17101 ccggtaatcc tttttgtcca taataatgca tattgacaag cattttaccc tctccagcga
    17161 tttggtagga aattgtcact tgggtttttg gtactgtgtt gatttccagt gtaaaaatga
    17221 tttcaaccgt ttcagcgtat tcattacttg aatattgatt atttactgga gcaatcggca
    17281 aatcgatctt tttattgtcc accagtactt ctatttcttt gacttttgga tacaatccgg
    17341 ctcctaacca cataccggat ttaaatccaa atccgctacc tctgtcatta tcggtcgttg
    17401 ctcgccaaaa aactggaagt gtttcccggt atagccattc ttttccgttt ttattgagcg
    17461 attccagtcc gcctcttgta taagaaaaaa tataatgcgt attccccgat ttgacaccta
    17521 atgtacagtc gccgaagacg attgcaactt tttccgtatc catatgactt ttatttactg
    17581 tatttttcat aataatatct tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa
    17641 acgatcccat tccctgagaa ttcatagtca cttggtttat cgtcaaagtc accgccatag
    17701 cgcatcactt ctctgcctgt gattggatca gtcacttgga tcgcctgatc tttaaaatcc
    17761 catatatagc ctccttggta catttcataa cgatcaagca acgtcatgta agaatccatt
    17821 ccacctaacg aattccccat gtcatgcata tattcacata agatgaacgg tttttccgct
    17881 ttattttcaa ggtagtcaat gatctcttct ggtggcgcat acatgcagct ttttacatct
    17941 gaaatcgaat cttcgaatgc tggatttgga aaattccctt cataatggac aagtcgatcg
    18001 gggtctactt ttttgaagta atcgttcatt tgtcgaatgt tttcttccgc ataagattca
    18061 tttccaagag accaaaaaag aatacttgga tggtttttta atgtttcgaa attcgttttt
    18121 gctcgatcaa gaacagccgc ctgccactga ggtactgcac ctggtacgtt gtaagaaggc
    18181 tcaaatgcgc ccattttttg ccatgagcca tgggattcga ggttcgtttc cgccatcaca
    18241 tggattcctg cttggtcgca taaataatac caagtgatct ggttggggta atgagatgtc
    18301 cgtacgccgt tgatctttgc attttttatg atagccaaat ctttttccat atcttctata
    18361 gtgatggcgc gtcctttttt cggatgccac tcatgtcgat ttaccccgtt gataatcatc
    18421 cgttttccat tgaagtagat gattttatct ttgatttcga ttttacgaaa accgaaggaa
    18481 tagtcaatat actccgttac tcttttttct tcgtctatcg tctccaattc aagcttatat
    18541 aaattgggct gaaaatgccc ccacagtgcc acattttcaa atccatcgat gcgaatcgtc
    18601 atttgctctt ttaacggata ttgctgttga tagatttcct tcccatcagg atctagtatc
    18661 gctacattca gctgactgtt tttggctact tgctcaaaag acaaatctac ttcaagtttg
    18721 ccattttgac atttgtcatc taagcgaggt ttgatccaaa gatcttctaa atggttttgt
    18781 cctcgagcaa ctaagaagac atctctgaag atcccaaaaa atcggaaaaa gtcctgatct
    18841 tccaagaatg cagctgtact tcttttgtaa acctccacag ctagaatatt ttcttcttcc
    18901 aaataaggtg ttaaatcaaa ttcagacgga gtgaacgaat cttcggcata cccgataaaa
    18961 tgaccattca accaaataaa cattgcttgt tctacaccag caaaatgaat cgagatttgt
    19021 tttcccttta gaccttcttc taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct
    19081 ttagcagctg aaaagatccc ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg
    19141 cgaaatacat gtccctccca cggatacatc gtattgatgt aatggatctt gtcataattt
    19201 gctaattcaa tatgactagg aacagtgatt tcatcaaaag aacaggcatc aaaatctttt
    19261 ttataaaatg tttttggtcg taccgaagga tttggcgaat aacaaaaacg ccatgatcca
    19321 tttaaagact gtttaaggga ggatgtggtt tctacttctt cccttgttgc ataacaaaca
    19381 tgatcgctat gtgcatttga ttggttcact ctaaaaactt gtggatcatc taaccatttt
    19441 atatttgctt tcatactttt ttccttctct ctcataactt tttgaaatga agcagttctg
    19501 tttggaaatc accttgaaaa tcggggaagt aaagtccaac tgtcatcaat tcggcccccg
    19561 aaaatatccg cttcgaggtc tgttcctgat aaagtgcctc atcatctaac cccatcagat
    19621 atacttcatg gaacgctggt tgagctgaag ctagtatcct ccctaaaaac acaatagctt
    19681 catcttgttt aggagacaca aacatccatg cggcaatatt ttcttcaaaa ggactcgcca
    19741 aacgataata atggccatac tggatcaccg gacgaatcgt ttgataggat ataatttggt
    19801 cttttacttg ctttttttct tcaggagaca gctttgtcaa atccagttca taaccaaaaa
    19861 cagcgctcat agctgtttct cctcttgttt tcaacgaagt agatctgtcg gtctgatgat
    19921 ttggtacagc tgatacatga gctgtgatgg acgaaatcgg ataagcaagg cttgttccat
    19981 attggatttt catacgctcc actgcatccg tgttatcact tgtccatgac tgcggcatat
    20041 aataaataaa cccggcatca aaccgtccac ctccaccaga acagccttcc cataagattt
    20101 ccggataatc agttgtcaaa tgttcaagta gttcataaag cccgagcata taacgatgcg
    20161 ctacttctcc ttgtctctgt gggtcaaggg taatcgaata gacatctgaa agcgaacggt
    20221 tcatatccca tttgatgtag tcaagaggaa tcgtatctaa aatctttctc atttgtttct
    20281 caatcgtctg ccgaacatct aaccttgtaa aatccagtac atgttgtgat ctggaagcag
    20341 aaggcattct acctggctcc tgcataagaa aatcaggatg tgttcgatac agttcagaat
    20401 caaccgagat catttcaggt tcaaaccata aaccaaattt caatcctttg ctatgagcat
    20461 aatctgctat ttcacgtaat ccatttgtca acttgccttc atactcgaac cagtctccta
    20521 atgaagaatt gtccgcatct ctttttccaa accagccatc atctaatacg aacatttcga
    20581 tgcctagctc ctttgcttca tcaatgatcg ccttgatttt ttcactattg aagtcaaagt
    20641 aagtggcttc ccagttattg accagaatcg gccgttcagc aaattgatga cgacctcttg
    20701 cgattctttc acgtagcaac tcatggtgga tctgactcat tttgttcaat ccctgacttg
    20761 agtaagaaag gatcatttca ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa
    20821 aacgatggct gttgatccca ccgacgactc tcaattgatt gatctgatct ttttcaagtt
    20881 cgaaagaatg gttgccggaa taaacaaatt gaagtccgat cgcttctcca gtattttctg
    20941 tcgtatgatg atgaactaaa gcaataaagt tattcatatg gtgactgctc gttcctcgac
    21001 ggctttcaaa tacttttgtt ccagataaaa cagattgccg tgaaatctgt ctttcattga
    21061 catgtgctcc tggcaaagca atgacttcat caaatcgcct agtatgggca aagtctagct
    21121 ggaaactagc cgctttttct agtttgattg tttctccggt ttcatttctt atctttacag
    21181 atcgtgtgat cactgctcta tcagtaaata tggtatagaa caaatcgaaa taaatcgctg
    21241 tttctctatc cttaagtgaa atcaccaacg tttgtgcctc tgacttgtcc atcacataag
    21301 tttgaggcaa gccttcgatc tctggcttcc catctgtaat ataatggctg tcatatctca
    21361 gatccagtaa gtctgatcca ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca
    21421 tgctgccatg agaagagtat tcttgcggta gtgtatcttt cgacagagac cggtcaggat
    21481 ttaagggaac atttcctgaa aatccacgat ctcttcgggg ataattttta ttgtctttgt
    21541 actgcttgac tggtttgcca aaataaatat gcgccaatac attgctttct tctatcttta
    21601 tcagataaga aagaaatgta tttcgtaaat ggaagactcg gctcttttca tcaaataaga
    21661 tgaaattttt atctgttttt ctatgcgtaa tatccatcat ttcactccca acaaaacgga
    21721 aaccgtttac ttttttttat aattcctgta tactgtttac taaacaattt tgtcaataaa
    21781 aattttttat ttttttgagg agtgtgtaaa aatggttgga attagagatg tcgcacgaaa
    21841 ggcaaatgtc tctcctgcta cagtttcccg tgtgttgaat aacgatcaga cgatccatgt
    21901 ggctcagact actcggcaac ggatagtaga ggcagctgct gcgttggatt atgacatcag
    21961 tactcgtaaa tatatcaaaa agagaatgcc ttcgattggt gtgatctcta cgatcggcaa
    22021 agcgagcgaa gaacaggata tctattacaa acatttgcga gtagggatgg aagaagaagc
    22081 tcgacgtctg catttaggga tgaatcgaat ctataatcta tctgataatc ctaaacaatg
    22141 ggaagatcta gatcagttag gtgcggtgat cgttgttggg actgttacga aagaatcgat
    22201 taccgccttg ttaaaacaaa acatgcaaat agtcgtcgtg gataaccctg acatccagca
    22261 agaggtcgat atggtgtatg cagatcttga acggatgact aaaaccgtac tggaattgtt
    22321 cttagaaaat ggtcataaaa gaatagccta tatcggggga tataaagtcg atatggatga
    22381 attcggtggg aagcagattt ctataaatga aaagaggatg cgtgcctatc agcattttat
    22441 gatcgatcat cacttagaaa aatatatgtc ttatcatcta ggtggctgga cagaaaaaca
    22501 aggtgaggta cttgcgacta atctattaga aaatgaatcc cctcttccta ctgccattct
    22561 tgtcgggagc gatccaatgg caattggtgt ctatcgggca ttgaaacaaa aaaatatcca
    22621 aattggagaa gagattgttg tagcaagctt tgataatatc gagacaatcg caaacctctc
    22681 ccctggcttg accagtgttc aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat
    22741 ggcattggag cgaattgacg gtatacggga cgaaccaatc attttgactt atcctgcacg
    22801 attgattgtt agggaaagtt ttatcccaag aacaaaaagg tattgaaaga gaggttgtga
    22861 aaagccgctt cgtttcttca tactcaaaac gaaacggctt tttcacaacc tcttacttat
    22921 acttattcac aaaatccaat gtctgctggt aacaattttg cgattctttt agcgtgtaat
    22981 caaattggta ttcatgggta atctcttttt ttgtatctgt ataaaacaag gattgcacgg
    23041 gtacctttaa ttctgtcaac cgattttcta atgcttgacc ttgctcctgg aatgagtatg
    23101 catttccgtc agttatatac gttggcggaa attccttaga aacatggtca gccacactag
    23161 cttctttcaa ttgctcactc gttttccaat cttttgttcc aatcaacgac cacgcaacgg
    23221 ttttgacgaa aaacttcata aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat
    23281 caacaggtcc gcaataagaa atggttcctt tcagactatc ttttggaagt agctgtttga
    23341 ttttcatttg tttagcatac gcatcattcg tttgtatagc tgcatattgc aatgcgattt
    23401 gtgaacctgc actatctcca ccaaaaaata attgttccat gtctagcaat ttgtcgtctt
    23461 tttcttcagc taataaatct ttgatcagtt cattcacttg aagaacttga ttagggtatt
    23521 ctgaatcagg cgctaattca taattcatcg caaccactgc gatgttggcg tctgaaacta
    23581 attttgtcgc aaattctttg acacccgatt tatctccgcc gacgaatcct ccaccatgta
    23641 cccaaaatag aacaggaaca ggccccttgc tgttttttgg atagtagatg tcataagtat
    23701 tctctttata cttggactca tacgtcaaat ctgttttttt cgccacgttt ttttctgctt
    23761 gatcgaacgt ttttttatca gtgatttgaa cagtatttga aaacatatgg ttgatcaaga
    23821 atgctccagg tctgggggaa agctggaagg caagaaagat tgcaaacgcg atcccaagaa
    23881 tgattccgaa gaaccatccg ataaatttaa gaattttctt tttcataagg ctccctcagt
    23941 agttagtatt aagcattctg attataccat agtgttattt cctatcaagc tctcctagat
    24001 gatttaatga ttttccccaa acttcttctg caatgctttt gacaaaagaa attttttcca
    24061 ttgttcttca aacaaaacat tttcttcttc tgttgaagca aatccacatt gaggacttaa
    24121 gcacaaacga tccaaaggca gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg
    24181 gtgctcttcc atttcgccat ttttcgttac agtcaaaatc taagacaact aatttatctt
    24241 cagagacttt ctctaaaaga acaaaactcc cagcacgttc actatcgtat tctaaataat
    24301 aggcacggac attttcttga gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg
    24361 ccgccaaaat cgaataataa tttccgcgtc aaacatgtat atttagtgtt gaatcatttg
    24421 agaatatgaa aataaaaatc tttttgatca atcagttaat caaattgaaa taacgaaaaa
    24481 tctgagccag aatgaggttc attctgaccc agatttgatt ttgtacaacc catcagtcct
    24541 tattgacaaa gagccactaa aaagactaag aaacaataac aaaagttaac acttcttagt
    24601 ctttcttta
//
LOCUS       DACCXO010000033        24356 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.034, whole genome shotgun sequence.
ACCESSION   DACCXO010000033 DACCXO010000000
VERSION     DACCXO010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.034"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(10..858)
                     /locus_tag="IVY70_001236"
     CDS             complement(10..858)
                     /locus_tag="IVY70_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7958373.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1082..3022)
                     /gene="thrS"
                     /locus_tag="IVY70_001237"
     CDS             complement(1082..3022)
                     /gene="thrS"
                     /locus_tag="IVY70_001237"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP7958374.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3359..3538)
                     /locus_tag="IVY70_001238"
     CDS             complement(3359..3538)
                     /locus_tag="IVY70_001238"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP7958375.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3585..5408)
                     /gene="cadA"
                     /locus_tag="IVY70_001239"
     CDS             complement(3585..5408)
                     /gene="cadA"
                     /locus_tag="IVY70_001239"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP7958376.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESAYAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5686..7965
                     /locus_tag="IVY70_001240"
     CDS             5686..7965
                     /locus_tag="IVY70_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7958377.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8058..8705)
                     /locus_tag="IVY70_001241"
     CDS             complement(8058..8705)
                     /locus_tag="IVY70_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958378.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8884..9603)
                     /locus_tag="IVY70_001242"
     CDS             complement(8884..9603)
                     /locus_tag="IVY70_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7958379.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9734..10885)
                     /locus_tag="IVY70_001243"
     CDS             complement(9734..10885)
                     /locus_tag="IVY70_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP7958380.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11102..11842)
                     /locus_tag="IVY70_001244"
     CDS             complement(11102..11842)
                     /locus_tag="IVY70_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7958381.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11846..12169)
                     /gene="rsfS"
                     /locus_tag="IVY70_001245"
     CDS             complement(11846..12169)
                     /gene="rsfS"
                     /locus_tag="IVY70_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP7958382.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12226..12819)
                     /locus_tag="IVY70_001246"
     CDS             complement(12226..12819)
                     /locus_tag="IVY70_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7958383.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12803..13450)
                     /locus_tag="IVY70_001247"
     CDS             complement(12803..13450)
                     /locus_tag="IVY70_001247"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP7958384.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13463..13774)
                     /gene="yhbY"
                     /locus_tag="IVY70_001248"
     CDS             complement(13463..13774)
                     /gene="yhbY"
                     /locus_tag="IVY70_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP7958385.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13790..14899)
                     /gene="yqeH"
                     /locus_tag="IVY70_001249"
     CDS             complement(13790..14899)
                     /gene="yqeH"
                     /locus_tag="IVY70_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP7958386.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14896..15423)
                     /locus_tag="IVY70_001250"
     CDS             complement(14896..15423)
                     /locus_tag="IVY70_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP7958387.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15603..16388)
                     /locus_tag="IVY70_001251"
     CDS             complement(15603..16388)
                     /locus_tag="IVY70_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP7958388.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16381..17250)
                     /locus_tag="IVY70_001252"
     CDS             complement(16381..17250)
                     /locus_tag="IVY70_001252"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP7958389.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17251..18630)
                     /locus_tag="IVY70_001253"
     CDS             complement(17251..18630)
                     /locus_tag="IVY70_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP7958390.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18638..19057)
                     /gene="fabZ"
                     /locus_tag="IVY70_001254"
     CDS             complement(18638..19057)
                     /gene="fabZ"
                     /locus_tag="IVY70_001254"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP7958391.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19082..19558)
                     /gene="accB"
                     /locus_tag="IVY70_001255"
     CDS             complement(19082..19558)
                     /gene="accB"
                     /locus_tag="IVY70_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP7958392.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19561..20796)
                     /gene="fabF"
                     /locus_tag="IVY70_001256"
     CDS             complement(19561..20796)
                     /gene="fabF"
                     /locus_tag="IVY70_001256"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP7958393.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20815..21552)
                     /gene="fabG"
                     /locus_tag="IVY70_001257"
     CDS             complement(20815..21552)
                     /gene="fabG"
                     /locus_tag="IVY70_001257"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP7958394.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21564..22481)
                     /gene="fabD"
                     /locus_tag="IVY70_001258"
     CDS             complement(21564..22481)
                     /gene="fabD"
                     /locus_tag="IVY70_001258"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP7958395.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22588..22812)
                     /locus_tag="IVY70_001259"
     CDS             complement(22588..22812)
                     /locus_tag="IVY70_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP7958396.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22865..23827)
                     /locus_tag="IVY70_001260"
     CDS             complement(22865..23827)
                     /locus_tag="IVY70_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP7958397.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23837..24277)
                     /locus_tag="IVY70_001261"
     CDS             complement(23837..24277)
                     /locus_tag="IVY70_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7958398.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6658 a         5428 c         4246 g         8024 t
ORIGIN      
        1 gtaaggtttt tatttcgaat ccataaaata ttcttggatt gcttctcgtg tttttttcac
       61 tttttcgcta agcttttcgt ttttagccat atagaaataa aaaatgatat cgatcgtagc
      121 aaattgggcc aaaagtgaat tagttgccgc acttcgattc gtgatttctt ttggtctaga
      181 tgtttgtacg agtacatcgg ctatcttgct aagcgggtta tttccaaatt gcgtcaacgc
      241 aatgatttcg atattcatgc ttttcgccag ctcggctaat gcgactacat ctgcacttct
      301 acctgagttt gaaacaagcc aaagcacttt ctttttctca ttgctgacaa gttgcgggag
      361 caagacgtgg atatcttttt caaaaatgat tggcttaccg acacgagacc atttttgcag
      421 aatatcttct actactaaag aagaggcgcc aaccccatat aaaaaaacag tctctgccga
      481 ttccaaacac tctacgactt tttggatcgt ttcttcttct agaatatcta ctgtttcttt
      541 tatcgctact tgtgcattgt taccaagttt gtttttgata gagtaaaagg actcatttgc
      601 ttctatatca ctaaaacctt catcgattcc agtctgtagt tctgttgaaa tcgtgatttt
      661 gaagtccgtc agactttgaa aaccgatttt tttggaaaaa cgtacaactg tcggcgcact
      721 caacccggaa gcttctgcta attcattagc tgtcattgta gggaccttct caatattacc
      781 gataatgtag ttggccaact ttttttctgc tgaagaataa tcttccatca tactttcaat
      841 acgactaatt acgctcataa gttcctccac ccttttaatg atagtctatg taagtatacc
      901 atatcagacc cttattcacg gtcgtatact ggataaaaaa caaaaaaaca atccgcccaa
      961 cagaatatct tatcccatca atcccacaga ttctattgag catctgtgtc tttgaggaat
     1021 acctgttatc tattgttcgg attgctttct atctactact tttttacagg aaatttacct
     1081 attagttttc tctgctgtaa tttttgactt ctgcttccat agcatcaata aagatattca
     1141 aatcttctac agctgtttct ttacttccat aacgacgtac gttcactgtt gcatcttcca
     1201 attccttgtc tccaacaact aattggtaag ggatcttttg tgtttgagag gcacgtatct
     1261 tatagcccat tttttcatta cgatcatcaa cttctacacg caaccccttc atttgcaaac
     1321 gttctttgat ttcataagca taatcgccat gtgcatcaac agaaacaggg atgattgttg
     1381 cttgtatagg tgccaaccat gttggaaaag cgcctttgta tacttctgtt aaataagcga
     1441 caaaacgttc cattgtagaa acaattccgc ggtggataac tactggacga tgcgtatttt
     1501 ccccgtcttc tccaacatat gtcaaatcaa agcgctctgg caataagaag tctaattgta
     1561 tagttgataa tgtttcttct gtaccaagag ctgttttgac ttgaacatct agttttggtc
     1621 cgtaaaaggc tgcctcgcct tctgcttcaa agtaatctag ttctaattca tccatagcgg
     1681 ctttcagcat tgcttgggct ttttcccaca ttgcgtcatc atcaaaatat ttatctgtat
     1741 tgtttggatc gcgatagctc aagcggaaac gataatctgt aatattgaag tcagcatata
     1801 ctgccaccat caattcaagc gtacgtttaa attcatcttt gatttgatca ggacgaacaa
     1861 atgtatgacc atcattcaac gtcatttctc gtacacgttg taaacctgac aaagcacctg
     1921 atttttcata gcgatgcatc attcctaatt cagcgatacg aattggtaat tcacgataag
     1981 aatggatcgt gtttttgtag accatcatgt gatgagggca gttcattggg cgaagaacaa
     2041 gcatttcgcc atctcccata tccattggcg ggaacatgtc ttcttgataa tgatcccagt
     2101 gtccagatgt tttgtacaac tcaacatctc ccattattgg tgtatataca tgttggtaac
     2161 ctagactaac ttctttgtct acgatataac gttcgatcgt gcgtcggatt gttgcccctt
     2221 ttggaagcca gaatggcaaa ccagaaccta cttctgggga aaccatgaac aagtcaagtt
     2281 cttttcctag tttacgatgg tctctttctt ttgcttcttc acggagacgg atatattctt
     2341 tcaaagcttt cttgtcaaaa aaggctgtcc cgtatacacg ttgcatcatt tgattgtttg
     2401 agtttccacg ccaataagca cctgccactg ataataattt aaatacttgt atacggccag
     2461 tggatggaac atgtggtcca cgacacaaat cgatgaattc gccttgttga taagcagtaa
     2521 tcacttcttc ttctggcaat tcactaatca gttctacttt gtacggatca cttgcaaaaa
     2581 tttctaatgc ttcctgtttt gaaagaacac ggcgttcgat cggcagattt tctttgacga
     2641 ttttcatcat ttctgcttcg attgccggca aatcttctgc agttacttgg ttttgtccat
     2701 tatctgtatc gtagtaaaac cctgaatcga tagctggtcc aacgccaaag tggatatttg
     2761 gatagaggcg acgcatggct tgggccatca aatgagctgc tgaatgacgg actaagccaa
     2821 gtgcatcttc atggtctggt gtgatgatct caatacttgc atcttcctcg attggacgag
     2881 tcaaatcaac gagttttccg ttcactttac cagctaaagc tttttttgct aagctttttg
     2941 aaatgctttc agcaattgcc aatgttgttg ttccagactc aaattctttg actgcaccat
     3001 ctggaaaagt aatttttatc ataatagttt cctccttgaa atgttttgat tgatcagatc
     3061 atcaagctga aaaacaaaaa aatcctagca tctcttgaaa agagatacta ggacgaaaag
     3121 ttcgtggttc cacctaatgt tcaaacgcaa atgcgttcct caagcgttgt aacggtccga
     3181 ccgcccttgc ttcaacaagg gtttcgaaag tggtcatcct ttggttttct cctggaaaag
     3241 tttcagccta gcttttcctc tcttatcgaa gactctcaaa ttcagttggc tttctcatca
     3301 atcaattatg tgtttattta accaccaata aatagaaaaa tcaagtggga aactaaactt
     3361 attttttcca ttccccattt acaccgtatg ttcctttttt catctcattg atgatgacat
     3421 ggatgtgttc ttttggtgcc tgcgtgtttt tatgaacagc ttccgtgata tctttcatca
     3481 tattttctaa ttgttccgga ctgcgacctt ctactaattc gacatgtacg aatggcataa
     3541 tgctctctcc tctttctgat cgttcttttt tcaactcgat tctatcatac tatgtttgtc
     3601 ttgggtactg aatcaggaaa aatggctgga tctttttttg ctctcaaggc actcaaaata
     3661 gagaccgtat ccacgacttc ttgcaaaaca gctccgaata atgcggggat tagtcccgta
     3721 cttgcaatta acatcaaaaa aatacaaata aagatgccaa tcagtacaga ctgtttcgct
     3781 attctcatcg tatctcttga aatcactact gcttttgcta cacgcaagag atcatctttc
     3841 aagatgaccg catatgcact ttcactagca gctgtagaac catgagcccc cattgcaata
     3901 ccgatatctg cagcagcaag tgctggtgcg tcattcacgc cgtctcctac catcgcaaca
     3961 ggtctttgat ctttagggat actttctaaa atggtcagtt tatcttgagg caagctacgg
     4021 gcatgtacag aatctaattg gactttttct gcaatcgttt gagcaatggc agatccatcc
     4081 ccggtgatca tcatcaaatt tttcacatca agttttctta gctccttgat tgtctttgct
     4141 gcttctgggc gaatctgatc cgaaaatagg atcgttcctg caaaagatcg atcaattgtt
     4201 acatagattg ctgtattgga cgtttctatt tcttcagctg aaacaaaaga ggcacgtcca
     4261 atctttactt catgattatt gactactgct tggattcctt gaccggtcac ttctttcagt
     4321 tgactaacag gatacagttg gctgtttttc acgttgttca ctaacgaacg agctaagata
     4381 tggctagatt cctgttctgc actagctgca tacagaagta gctcttcttt ttttattcca
     4441 ttgactggct gaatcgcctc tacttccaat atccctttgg taagcgttcc tgttttatca
     4501 aaagcaatcg tttttgtacg tgcgagcttt tcaatcgttg ttccagtttt tacaacgatc
     4561 ccgtttttac ttgaacggct cattcctgca actaaagcaa tgggcgcagc tagaatcagt
     4621 ggacaaggtg atgccactac cagtacctca gcaaatcgta aagggtcttt tgagaaccac
     4681 caagaaatac caccgatcaa ataagcaatc aacgtgaaag ggactgcata acgatcagct
     4741 aaacggacaa atcgggcagg cttttctttt gattcttcga ctagtttaat aatcatttga
     4801 tactggctgt ctgcggcacg tttttcagcg atcatttgga tcgatccgtc tccatttaaa
     4861 gaccctgaca taatctgatc tccaggtttc ttggtttccg ggcgggactc acctgtcaaa
     4921 gaagattcat cgaccgaaga ttctcctttg gtcactcgac catctgcggg tacaagttcg
     4981 ccaggcttga ctagtaactg gtcatcaata tttatttcat cgattactat atcctgcaat
     5041 tttccttctt tgaatcgatg ggcaattctt ggggtgttat ctaacaaaga ctgcagttct
     5101 ttacccgctc gtttattcgc gtaatcctct agactatctc cgcccgtaag catgatcaat
     5161 atcatcaaac tagcccagta ttctttgacg gctaatgttg caaggattgc ggtaatcgct
     5221 aatacatcta cgccatatct tcctgaacgt aacgtcttga tcatttccaa aaacatcaag
     5281 aaggcgagaa tccctccagt aaatgcgaca atcgcaaatg caagggccgg ctggtgaaaa
     5341 acaaattcac ttaataaagc tatcccacca ataagcaaag taagtaaaaa ttttttgaaa
     5401 tgtgtcattg ttttttctcc tcttttatgt gacttagaga ggttagtaat aaatttccag
     5461 ctgcctcttt catcttaagc atagtccttg actagcactt tttaaaacaa taggccttaa
     5521 ttgaaaacaa ctatcactat cagcgtttcc aaaattcatc agttctacaa acaaaaatat
     5581 tcagcaagga ttactttttc gattcatcag ttaaaaagta ccttttttcg aaaaactatg
     5641 ctatactttt aattcgttat ttacggcgga aggagcaaac atgcgatgaa tgagaaacaa
     5701 ctagaacaaa cgcacgtgga tgaaacgatc gaactaatcc agatcgaaca aaatatgctg
     5761 gatacaaaac aacaagaatt gactcaggaa atgaatcatt cgacaaaaga cacagctaat
     5821 cacaagattc gagctggttc caatgaagct ttttatgaat ctgctgttga atatcgccaa
     5881 catgagcagg aattgctttt gaaataccat actcttgaag cacagcaaaa acgcctgcaa
     5941 acgttgtctg tcatgaaaga tagtccttat tttgcccgta tcgactttaa agaagaccgt
     6001 gaaacagaaa cattatatct aggtatcgct tctttaagag atctggcaga agaaacaatc
     6061 gttattgact ggcgcgcacc aattgccaat ctttattatg aaggtgagat tggtcccgca
     6121 ttttatgaaa ctgaaacaga tagaatagat gtcgatctgc agttgaaacg acaatttaaa
     6181 atccaagaag gacgtatttt atctatggta gatacgtcgg aagtaatcaa tgatgacttt
     6241 ttgctagaga tattggatga atcatcaagc aaccatatga aaaacatcgt ctctacgata
     6301 caaaaagcac aaaatgcaat cattcgggac acaacaagta aagtaatgct gattgaagga
     6361 atcgccggaa gtggaaagac atccgctttg cttcaacgta ttgcttttct gttgtatcat
     6421 aatcgcaagt ggctcgaggc tgaaaacgtt cttctatttt cacccaacca tttgttttct
     6481 gattacatct cgactgtatt gccttcatta ggtgaaagcg gcgtgcctac acagacattc
     6541 aaaaattatt tggtgcagct gctgccagag ttttctttac tagatgaaca gcaacaagaa
     6601 agtggttttt tgctagggga aaaagatccg atacaagtga tgaaaagcgg attgacctta
     6661 gtcgatcaga ttgatcgcta tatccaaggc atcacaaact acggtccctt gttccgtgat
     6721 atgaaaataa atggccgtac gatcctatca aaggaatcga ttcgccaatg gtataaagaa
     6781 acaaatgaac aattaccttt acatcataga ctttctctat tacaaacgaa gctcctgaaa
     6841 aaattaggcg gtctgcaaaa agacgaaaca cgtcagcaat gggtcaaaga tctggcggaa
     6901 gaacagctcc aagaactata cgcaacagat ccaaatcttg aatatacaga acaaaaggag
     6961 cgttcattac gtaaaaaact ggccaaccaa atcgtacgta aaagattccg tcgaatccat
     7021 cgagggatca tgaattatca atttgtcaat ataacgaaac aatatctcca ctttctacaa
     7081 actgtgccac tttctcttct aacacgacat aaaatcagcg agaccaggtg gcaggaacat
     7141 ttgacaactg ttcgtacaca cttgcgtcaa agagaattgc gtcaagagga tgctgtacta
     7201 ttctttttac tgatgcgccg aacatatcca gtgacggtta cacacaaagc tcgctatatc
     7261 tttattgatg aaatgcagga tttcgcacca tcacaagtgg cattgctgca agccttgttc
     7321 ccaaaagcaa atttgacttt ctgtggagat ctaaatcaaa atgtctttgg aaatgaaacg
     7381 atcgttggat cattggaaga ccttttcgtg gaccgtgaag tcactcgttt ccaattaacg
     7441 acaagctatc gctccacaaa agaaatcact gattttgcta atcattttat tgtgaaagaa
     7501 catcaagtag aaacaacagc aagaaaagga gaccttcctt tgctcatcca aggaacaaca
     7561 gatgatatca aattgaattg gttagcagat accattcgaa cgactcaggg aaaagcccgg
     7621 tattggcgta cagcaatcat agctaaaaca caagaagaat gcgaagctct ttatgctcaa
     7681 ctgccagacg agttgaaaca acaagtccag ctgattttaa atgagagtga ctttatgaag
     7741 cgttccatta ttttacttcc agccttttta gcaaaaggtc tggagtttga ccgagttttc
     7801 ttgtgggatg tagatgcaga acatttttct tctcagcaag acaaattgat cctttacacc
     7861 atgtgtacac gtgcgatgca tgaattgacc ctttttacct ccaataatga aagtcctttg
     7921 ttaaacaata tggatatttc atcttatgag aaaaaagtac tgtaagtaaa aagacaactg
     7981 ctatcattaa tgttagcagt tgtcttttta tttacaataa taattgcaga aaaagtaatt
     8041 caatcattta aggtcattta ttctatatat aaatccaaat ctgtttcagg atattttaat
     8101 ttgacaagcg aaataatcat tttcagttct tccatgtttt ctggatattc tgggtcaaat
     8161 accttgctat gacggccaat cccgccattg tcgatcaatc ggtaaattcg attccattca
     8221 atgaattggt caattgattg gaaattgctg attggatagc cgccacaatc caaaatctct
     8281 gatacacgaa atgctaggta tgcactatcg atcactttat tttttttcgt gtctatcttc
     8341 tcatttcttt cgataagaat tttttcagga cgatgtttca agctccactc taatagtaga
     8401 aagaaagcct ccatccgata ctcttcttca aataaccgaa aggctgaatc agaacgacga
     8461 aaaagcagcg gaatcagata attttgtcta taaatcagca ttggtgcgcg gtccaccata
     8521 cactctaaat aacggttcag ataccctgta ccgacatagt ttcctaatcg atagccttca
     8581 acgccgaatc ttgcgtattt ttgcgctaat tttgaacaat cagttacgtt cacagctaac
     8641 tcttctactt tgcggtaacc gcttaagata cgttcaaaag gttctaaagt gatttttgct
     8701 tccatattct tccccgtcca tcttccacat acttcatatc atttcacatg atacttatgc
     8761 tctcatcata ccacaggttg aattagaaaa gaaaataaat cacacaaaaa agcaagacaa
     8821 tctgttagtg agattgtctt gcttttcgaa aaagactgaa tacttttcgt taaaactggt
     8881 tgcttagtct tctaatgcca cattatggaa cacatgctgt acatcttcta gatcttccag
     8941 tgcatcaatc attttttcaa atgtttcttg atcttctgga gatagtgtta cctcgttttg
     9001 agggatcatt tggatctcag cgattgaaaa atcagtgatt cctttttctt ttaatgcttc
     9061 ttgtatacta tggaaatccg ctacttcacc gtaaacgatg atttgtcctt cttcttccat
     9121 tacatcgcga acatcgatgt ctttttccat taggtattca aggatctcgt cagcgtcttc
     9181 tcctgcaaaa gcaaagattg ctgtattgtc aaacatgtaa gaaaccgctc cggaaacacc
     9241 catgttcccg ccatttttcc caaatgctgc ccgtacgtct gctgctgttc gattcacatt
     9301 gttcgtcaat gtatcaacaa tcaccattga gccgcttggt ccaaatcctt catagcgcaa
     9361 ttcagagtat gtttcatctc ctgcaccttt tgctttttcg attgctcggt caataatatg
     9421 ttttggtaca ttataagttt tagcgcgatc aatgacgaaa cgtagttttt ggtttgcatg
     9481 cggatcagga tcacctgatt ttgctgccgc gtagatttca atcccaaatt ttgcataaat
     9541 ccggctatta ttagcatctt tagcagtttt ctttgctacg atatttgccc atttacgtcc
     9601 catattttca cttcgctttc tgatttgata gtttcataac gcaaaaacgt cctgttccat
     9661 tatacttgtg aatcacgatt ctgtgaagct ttttttcttt cttccttcat tcttctcatt
     9721 tttagtcaat tatctatact cggattggga tttgcccgaa attctgttcc ggaatattgc
     9781 ctcccaattg atagatttga tcacttctaa ctaacagttc cgcttcttgt gcctcttttt
     9841 ttccgattcg ggaataaata gaaatctctt ttttttcttt taagaatctt cttccagcat
     9901 cggtaaatcc aagtagtcga agataatttt tattttgttg gacaggaatc tcttgctttt
     9961 tgatattcag caagacataa caagataatc gttggatccg tgtccatgta tatctttttg
    10021 tttttacctt tgacacaaaa tcctcaaaac tatcagcagt tgcagctgct gttttcatgc
    10081 gcgattctat tccttcggtc atctgataga tatctgtcag ttccagcaaa gtagaagata
    10141 acaaccgata acgcaaatac ggccaataag attcccaagt ctgcagcgaa caagttgaaa
    10201 gatcttcttc tgtctgaggc ggcacaagtc cagctattct ttttccttct tctaacgcat
    10261 ggcgaatggc tgttgcactg gctatcctct gatctgtgat gattttttca tgatagcctg
    10321 cacttttacg tcggatcgga tgtaattcca tagggtggac atacttggca ttttcctttg
    10381 cataacttaa tcccaagata tgatttggcg aagaaaaatc aagcgtaata tcgggataca
    10441 gactgcgaaa aacctcagtc attttttgcg gataactata cgtttgatca gacagttctt
    10501 gaaatgcctc ttctatccta tcttgattct cttggacaaa gcgtccgaaa ctgctgtaat
    10561 caaacgaagt ctcttcttct gttccaaaac acaaactgtc acagtccagt gtttgcaaga
    10621 tttttactgc acctttagca aaatagtcag ctgactgaac cgcccattcg acgggaagct
    10681 ccactactaa atccacacca ttttgtaaag cttcattcgc gcgatgccat ttatcgatga
    10741 tagccggttc gcctctttgc aaaaaattcc cgctcattac agcaatgacg acctctgctc
    10801 ctgttatttt tcgtgccatt tccacatgat agcgatgtcc attgtgaaaa ggattgtact
    10861 ctacgataat cccgcagctt ttcatgcgca tcctccttca tagttatcga taaaaaagaa
    10921 atagagacaa gtaaaaaaag gcaaaaatcg gaaaaaatac ccgaactata taaactattc
    10981 tcgcaaacac aatacttctg cgacgaaaag ctgcactgca agagcaatgc ctatatcgtt
    11041 cggatatata atttatgttg aagatttatt cttttcttgt taactcgagc aggcaaaacc
    11101 gctagtcttt gtatgctaca aagaaccagc gctttgattt ctctgatgct gcttcgtctg
    11161 taaaatcaga aaatgcttcg actttcgaaa agccgctgtt ttccagcatt cttaaataac
    11221 tttccaatgg ataggtacgc tcttgatgta gttcatcttc acgaataaat cgaccagacc
    11281 cttcttgttc ttcaacgaaa aaggtaagaa aatgttcgat gctgtgtttc tcgcttcctg
    11341 gataactatc ccacaaaaaa gcaaaattat ccccttgata atgatagcta tattctggaa
    11401 aaacctcatc tacttgatag gtggagtgta cgtcaaataa aaatcggcca ttttcttcta
    11461 acgcttgata gacttcatca aacacttgct gtacctcttg tctatttttc atatagcaca
    11521 gcgaatcaga aaaacaggta attgcctgat attgtccgat ttctgataaa tcgagcatat
    11581 tgccttccac aaattggatc gatgcgtctt cttcatatgc tcgctggctg gcaatcatca
    11641 acatctcttc tgaaaggtct aaccctgtca ctttatatcc atcttttgca aaggcaaccg
    11701 ctaatgctcc agtaccgcat gccagttcca aaatattttt tgtgtcttct tgtaaatgac
    11761 gtttcgaaaa agctaaccat tgctgataaa gtgtttcgtc catcacttca tcgtaaatga
    11821 aagcaaatgt ttcataagcc atatgttaat ccaaccattc actaagatcg actagcggag
    11881 catctgacca tagtttttcc agattataga aatttcgttc tggcgcatgg aaaacatgta
    11941 cgatcacatc tccaagatcg atcaataccc attttccgcc ttcttttcct tcaatacgtt
    12001 ttacttcata tttgttttct tcttctttat caatgatttc ttctgtaatt gcgttgattt
    12061 gacgttcgct atttgctgaa cagatcatga aataatctgc caacaaggaa acatttcgaa
    12121 cgtctaaagc gataatgtct tctgcccgtt ttgaatctgc tgcttcaatc gcaattttta
    12181 attgttcttt actatctatg atgatttcct cctaatatga gcagcttatt tttttgctac
    12241 ccagtaatta tatgtttcga ttgtttttgg ataaatctgt tgttcttgcg caagcaaatg
    12301 ggccaatgta tgttttgttt caaatgctac agcttcgtct agatcagccc atgcaatggc
    12361 tcgtgcatct tgtaccccag gaaaatctcg acctggttca atatagtctg ctacataaat
    12421 gattttgtca aggagactca tcttagcagc cccagtcgta tgcacccgga ttgcatgaag
    12481 aatctctgca tcggtgattc ctaattcccg ctctacaaac gaagcgccaa caagaccatg
    12541 ccagattgca ttattataat tcaataactc cggatcaaaa ccgtctctta cgattaccat
    12601 tttgaactcc tcatctggtc gttcttttgc ataatcatgt gtcaatgcag caatactagc
    12661 tttttcagga gaagcaccgt attttttggc aagagccacg gctgtttctt ctactccaag
    12721 cacatgcttg aagcgttgtt cgctcattct catctgtatt ttttgcatca ggttttctct
    12781 agaataagca gtatattcat tactatagtc catattcata caacccttca ttttgaatat
    12841 aatcaattac tttatctgga acaaggtagc ggatagaaca gccttctttg atcttttgac
    12901 gtattttcgt ggaactgatg tcgatctcag gtacatctac ccaaattaca ggatatggag
    12961 tatccgttgt gtatcctggt cgacgtatac ccacaaagtt gaccatagaa gtcagctcat
    13021 cgattttata ccattttggc aaatattcta ccatatcccc accgataata aagtaataat
    13081 ctgtatccgg attgttttgc gtcaattcct tcatagtatc ataggtgtaa cttttcccac
    13141 cacgagctaa ttcaatcgtt tcaatctgca aaaaaggatt gtcttccacc gctaattcca
    13201 acatgttgag tcgatgtttt gcatcaatcg tctgtttctc atcaacatga ggtggctgat
    13261 aagaaggcat caaaaatacg cggtcaagac ctaaattccg gccagcttgt tcagccatca
    13321 ctaaatgtgc aaggtgaacg ggattgaatg ttccgcccaa gatccctact tgctttttct
    13381 tctctaagaa ttgaggcatt tcttcaggaa ataattgtga acgaacaaat gtcttagctt
    13441 gcgtattcat catggtcact ccttatattg cttttacttc ttttgatagc ctttgatact
    13501 tttcttttgt ggaaggtttg tataatacaa taacacgtcc aatgatttgg acgatttcgc
    13561 aatctatttc tttttctaat acttgagctg cttcttctgc aatctcatct gtattttgaa
    13621 gtaacgatac tttgatcagt tctctttttt ctaaagcttc ttcaatttga acgatcattg
    13681 ctgaattgat cccacctttt ccgatttgaa aaatcggctg aagatgatgc gctttgctac
    13741 gtaaaaaacg cttttgtttt cctcttaaac tcataatttt ccttcttcct taaatcaatg
    13801 ctttgcgttt caatacatct acaccctttg gcgcccagcc cgcaattact actggcgctg
    13861 ttacagtgat ccagcctaga ccggcaaaaa cgatgtctgt tttttctttc acagaaaact
    13921 caaaacgaac aagctccgga aatgcctcga cttcatcagg acgaggaggc tgtaataatc
    13981 cacctacatg tttttgataa aattcgtctg ctttttccag tttcgttcga tgaatattta
    14041 catcatttga cacataagca ataaatgaag cacgattgcc gctgataaaa tcaaatcttg
    14101 caagtccgcc caagaaaagt gtttgccctt cattcagctg ataaacttta ggtttgattt
    14161 cttttaacgg tgcaatgatc cgcaaatctt ttttacctaa gtagtgcgcc atttgatggc
    14221 gatggataat tcccggtgta tcaatcaaaa aatgcccgtc atctaaagga atctcgattt
    14281 tatccaaggt cgttcctgga aactgcgaag ttgtgattac atcttgtact cccgctgtat
    14341 ttttgatgat ttgattgata agcgtcgatt ttccgacatt tgttactccg acgatataga
    14401 catcccgtcc atcgcggtat tcttcgattt tttctaatag ttccgtcatc tcatgagatt
    14461 tcttagcact agttaataat acatctactg gacgaattcc tgcttcgtaa gcacgttcct
    14521 tcatccattg tgttaattta gtttttttca atgatttagg taatatatcc actttgttcc
    14581 caactaaaag tactggattg tcaccgacaa aacggtgtag tcctgggatt agtgaaccat
    14641 tgaagtcaaa aatatccacc acattgacga tcaatgcatc tttttgccct aattcattca
    14701 gtaagcgcaa aaagtcgtca tccgtcaatg agacatcttg ggtctcatta taatggcgta
    14761 atctgaaaca acgctgacaa tagacttccc cggaagctaa acctttttcc aaagccgttt
    14821 gcggcgtgta tcctaattca ccgggtttgt ttgtttgtat aacggcacca cagccgatac
    14881 attgtaattc ttctgtcatt cgattccacc tcgccagccc atatcagggt gttttgctaa
    14941 tagataggcc ataattcttt tttcccaaaa acgattaaat tgtgtcttcc aactatctgt
    15001 tgcgataatg ggttttacaa ggatgctacg tatccctgca cgattggctc cacgtatatc
    15061 agtcataatc tgatcgccta ccattactat ctcatcatct gctaaatcca attgtttctt
    15121 tgctatgttg atccctcttg ctaaaggttt cattgctcgt gcaacataca aaagatcaaa
    15181 tttttcaatc gctcgcgcga cacgttttga attattgttt gaaacaacaa caacagggat
    15241 tcctgcattt ttcatttcta aaatccaagt aagcaactct tctgttccat ctggattatt
    15301 ccatgcaatc aacgtattgt ctaaatcggt caatactgct ttgatgccta gattctttaa
    15361 ttgggcaggt gtaatctgat agatggcttc taccatccat gttggtttgt aatttgaaaa
    15421 catgctgtct cctcatctgc aaaaaatgaa ggagcacata aagtactcct caaccccgta
    15481 ttatactgca tatcagcaaa aaaaactacc tctatactcg ttcgtctttc agaaaaagca
    15541 tttctttaca aaatgaccaa aaataaagat tgatcacaga aaaatttaga aacaaaaaac
    15601 aactaatatt ggcggaacct cttgtatcgt ttttccagta atttatgctg atctaatcga
    15661 acaagttccg tcaatctttc aatcaacgct tttttcataa tgtgtgcgac tttatatcgt
    15721 ggcaactgct cgccgttgaa cgtttcagga ataactcgat caacgattcc taactcgcgt
    15781 agttctggcg cagtaatttt catcaactca gctgcttctg cggcgcgact accgtctttc
    15841 cacaaaatcg acgcaaatcc ttctggagaa agcacagcat aaatcgtttc ttccagcatc
    15901 cagacttcat ccgctaaacc tagagcaagg gcaccgccac ttccaccttc accaatgatg
    15961 atggcgatga tcggtacctt taaatcagac atttcgagca gatttctggc aatagcctct
    16021 ccttcgccgc gctcttctgc tcctacaccg caatatgcac ctgctgtatt gatcaaggta
    16081 atcaccggtc tattgaactt ttccgcttgt ttcatcagtc gtaaagcttt tcgatagcct
    16141 tcaggatggg gagaaccaaa atttctgcgg atattttcct gcagatcccg tcctttttga
    16201 ataccgatca ctgttaccgg tttgccatct aatcgagcga ttccaccaac aatcgcttcg
    16261 tcatctgcaa ataaacgatc accatggcat tcctcgaaag aatcgaagat ctcttcaaaa
    16321 aaatccaagc ttgtccatcg ttctttagct cttgctaact gaacgacatc atgtgctgtc
    16381 ttatccacga tctatccatc ctttctgtgt atgcaattca agaagcgtat ggattttctg
    16441 tttcaattcc atgcggggaa caatctgatc gacaaaacca tgagataata agaattcagc
    16501 cttttggaaa tcttctggaa gttcctgctt gatcgtctgt tcgatgacgc gtcttccagc
    16561 aaaaccgatc aagctttggg gttctgctag aataatatcg ccttccattg caaaactggc
    16621 agttactcct ccggtcgtcg ggtccgttaa aacagttaga taaaaaagac cttcattgct
    16681 atgccgtttg acagcagcag agatttttgc catctgcatt agagaaaaga ttccttcttg
    16741 catccgtgcg ccacctgaag ctgtgaacaa gacgactgga agactttcct ttgttgctcg
    16801 ttcgaataat ctggtgatct tttctccgac aatcgttccc atacttccca tgataaaatt
    16861 aggatccatc actccaataa tgaagggaat cccctgaatg gttgctttac cagtgaggac
    16921 tgcttcatga agtcctgttt tatcttgcat ttttttaatt ttttccgtat attcgggaaa
    16981 tgctagcgga tctttggtta caagatccgt atcccattct tcaaaacttt tttcatcaat
    17041 aatcaaagct agacgctgcc atgcaccgat tcgaaaagcg tatccgcaat gagggcatac
    17101 tttttcttca ccgatgtctt tcgtataaag gatatgtttg caatttggac actttgccca
    17161 catgttatca ggaacagatg gcgagttagc aggtttgctc tctctattag gattgatccg
    17221 aatatatttc tttttcttaa acagagccat ctatgcgcct cctatctccg tttctggtgt
    17281 ccaattcggt aaaaattctt cttgtaaaaa tgctgtgctg taatcacctg ctatgactgc
    17341 aggatgacta atcaaatcca tctgaaattc tgcgttcgtg atgattcctt ctgtgataaa
    17401 ttcacttaag gctcgctgca tcttcattaa tgcttcaaaa cgcgtatttc catggacgat
    17461 tatttttgca atcattgaat cataatatgg cgggatcgta tatcccgggt aaactgcact
    17521 gtctactcgc agtcccatcc cacctgctgg aagcagtaag ttttggatct tccctggaga
    17581 aggagcaaaa tggaaagcag gattttctgc attaatccgg cattcgattg cgtgtccttg
    17641 gaatactact tcttcttgac acacattcaa aggctcaccg gctgctatct ccacctgttt
    17701 ttttactaaa tcgattcctg tgaccatttc tgttactgga tgttccactt ggattcgagt
    17761 attcatttcc atgaaataaa aatcgccttc ttcatccatc agaaactcga tcgttccggc
    17821 attttcataa ttgactgctt tagctgctcg aacagcagcg tctcctagtg cttctctttt
    17881 tgtttgtgag atcactacag aaggagattc ttccagtact ttttgattgt tgcgttgtaa
    17941 agaacaatct cgctcgccta aatgaatgac atgaccaaat cgatcaccta agatctgaac
    18001 ttctatatgg cgagcaggat aaatgatttt ttccaaatac atatcatcat ttccaaatgc
    18061 cgctgctgct tcttgctgag ctgaagaaaa atgctgtggg agctcttctt ttgattgaac
    18121 tttccgaatg cctttcccgc caccacctgc agctgctttc agcatcactg gataaccaat
    18181 tctgtctgct atttccaatg cttcttctac tgtagataat acaccagtac ttcctggaat
    18241 gactgggaca tttgcttctt gcatcaattg acgcgcattg atcttatttc ccattgcatc
    18301 gatcgttgca cttttaggac caataaatgt gatattgcac tcttcacaca tggaggcaaa
    18361 tcggctattt tcagataaaa aaccaaatcc tggatgaatt gcttcggctt ttgtcacgat
    18421 tgcagcgctc agtacttcct gaacattcag atatgaatca acggcttttg ccgggccaat
    18481 gcagattgct tcatctgcta actgtgtatg aagtgcttcg gcatctgctt ctgaatatat
    18541 cgctacggtt tggatcccta gttcgcggca tgcacgaatg atacgtacag cgatttcacc
    18601 gcggttagct atcaatactt tagaaaacat atttcaccta tccaatcata aaggttaatt
    18661 ccgcttcggc tactttttta ccgtctactt tagcgattcc tttgcctatt ccggctgaag
    18721 ctttcacttt caatatctca acttcaaggt ataaggtatc tcccggtgta actttcttac
    18781 ggaattttgc tttgtccaat ccgccgaaat aagccgtctt tcccttgaat tgttcaagag
    18841 aaagtaatgc aactgctcct gcttgagcca ttgcttcaac aatcaatacc cctggcataa
    18901 caggttcttc tggaaaatgt ccttgaaaaa agggctcatt gatcgttaca tttttcttag
    18961 caacgatccg ttctccttca accatttctt ccactcgatc aatcaaaagc attggataac
    19021 gatgtggaat gatttctttt atttcttgta tgttcattgt tttccctctt tcctgctttg
    19081 cttatttttg ctgaatccta aagagtggct gtccatattc gacaactgac tcgttttcta
    19141 caaggatttc tgtgatcact ccgtcggcct cactagttat ctcattcatc agtttcatcg
    19201 cttcaataat gcagataact tctcctttgg caacttgatc acctactttt ttgaaagctg
    19261 gtttgtcggg cgctggttgc agataaacga ttcccacgat tggcgaaaca acctcgattc
    19321 catcaaatac tgccgattct ttttctttca ttatttctgg agcagttgtt gcttgttcag
    19381 gtatgacatt gactggctcc gaaactggca acgtttcgtt tgtttgaacc atattcatgc
    19441 ctcgacttgt tttattttta ttcatataca attcaaattg accttcacgc aaatcgaatt
    19501 ccgttagcga ggattgatca aattgactga ctaattctct gatttcattg atatccaaaa
    19561 ttattcctcc cagcgtttca tgcacagaac cgcattatgt ccgccaaacc ctaatgaatt
    19621 actcattgca taagtaaaac ttgcaggttt gctttcgttt ttgacaactg tcaagtcaat
    19681 ctcaggatct tgtttttcta cattgatcgt aggtgggata aattgatgtt ctaatgccag
    19741 caaagttgcc aaggcttcga tcccgccggc tgctcccaat aaatgtccag tcatcgattt
    19801 cgtgctgctg acgtaagtat ctctgaaatt ttcacctaat gcgatttgga ttgcttttgc
    19861 ttctgcctgg tcattggctg gtgtacttgt tccgtgagca ttgatatatc cgacttgttc
    19921 cggctgtaca ccagcttctt ccatcgcaag cttcattgct ttagatgcac cagaaccatc
    19981 tggagtagga gctgtcatat ggtaagcgtc acagtttgca ccgtatccaa cgatttctcc
    20041 atagattttg gcaccgcgag cttgtgcatg ttctaaagat tcaagaacta gaacgcctgc
    20101 tccttcaccc atcacaaaac cattccgatt ttcgtcaaac ggaatcgaac cacgattagg
    20161 atcagttgat tctgagagtg ctgttaaagc tgcaaatcca gcaatcccca tctcacaaat
    20221 cgttgcttct gttcctccag caagaatgac atcagaatag ccatgtttga tgttacggaa
    20281 tgcttcccca attgaattcg ttccgctagc acaagctgtt acgatagctg tacaaatacc
    20341 ttttgcacct acacgcaaag caatatttcc agctgccatg ttggcaatcg ccattggtac
    20401 gaacattgga gaaactcgtt ttgcaccttt ttcatgtaga cgaataactt ggttttcgat
    20461 tgttggaaga ccaccgatcc cagatccgat gatgacgcca aaccggtctt gattcatttt
    20521 ttctttatct aaaccgctca tctccattgc ttctaaagca gcgtagattc catagatcga
    20581 aaacatatcc attcgtttgc tatctttttt aataaaatat ttatcgtaag ggaaatcttt
    20641 gacttcaccc gctaaggtaa tccctgtttc tgaagcatca aatttagtga tcggtgcgat
    20701 gccattcgtc ccctctttta agctattcaa aaagctgtct gcgtcatttc caataggtga
    20761 agtgactcca tagccagtaa ttaccactcg attcatttgt ttgctctcct tttcttatcc
    20821 gtgcatgacc aatccgccat cgacattgat gacttgtccg gtgatatatg gattttgtgc
    20881 taagaaaact gctgtttgtg caatatcttc tacttgtccg aaacgctgca tcggaatttg
    20941 cttttccgct tgttccttga ctttgtctgc taaaacttcc gtcatatcag ttgtgatgaa
    21001 tcctggcgca atggcattac atgtaatgcc tctagttgca gcttctcgtg ccacagattt
    21061 tgttaatcct actacgccag ctttactagc tgcataattt gcttgaccaa tatttccaat
    21121 caacccagat acactagaga gattaatgat ggccccttct cgctgcttca tcattttttt
    21181 caatacgtgc tgcgtcatat tgaaggttcc aatcaaattg atatccagac attttttgaa
    21241 atcttctgca tccattcgca tcacaagttt gtcatttgtg attccggcat tgtttactaa
    21301 cacatggatc gatccaagct tttcttctgc ttctttgatc atttgtcccg ctttctcata
    21361 atcagagatg tcaccggaaa ctcctacgca ttttacaccg aatgcttcga tttcttctat
    21421 cttttctttt ggaatctctc cgcgaccatt caaaatgata tttgctccag cttttgcaaa
    21481 agctaaggcc atcgctttgc cgattcctct tgtactacct gtgataaata cattttttcc
    21541 ttttacatcc ataacgttcc tccttatagt attgctattg tttcatcaaa tgtctgctta
    21601 ttctcaacac gcagaactgg tattgtttta tcgatttttt tcataaatcc gcttaatact
    21661 tttccggggc cgacttcgat gacttgttcg atgccgattg tttttaactt atggatactc
    21721 tcatagaaac gaacaggttg catgacttgc tgttcaagta gtcccgcgat tgtttctttc
    21781 ttcattattt ccgttgttgt gttgcttatg ataggaaaag aaggttcaga gaattgaatc
    21841 tgacttaggt ccttcgccag tttttttgct gccggttcta aaatagctgt atgaaaaggc
    21901 ccgctgacat ttagagggat cattcttttg aacccttttt cttttaatag taaaacagct
    21961 tcgtctactg ctttttcttc gccaccaatc acgatctgct gaggtgtatt ataatttgca
    22021 ggagatacga tgccgtactt gctagcttca tgacagcttt cctcgatcgt ttcaatcggc
    22081 gcattcatca ctgcaaccat tttcccgctt ccagctgggg cagcttctgt catataagcg
    22141 cctctttttg ctactaacgc aactgcttca gtgaaggata gcgcattact agctactaat
    22201 gccgaatatt ctcctaaact cagtcctaaa gctgcatctg ggatgattcc atgctccttc
    22261 aataagcgat aatacgcgat gctgactgtt agaatagctg gctgtgtata ttgcgtctga
    22321 tccaaacgtt cattttctgt aaagcaaagt tcagccatat cgtagccgag aatctcactt
    22381 gcttcatcaa atgtctgctt tacaacggct tcttcatata aatcttttcc cataccttga
    22441 tattgtgcac cttggccact aaataagaat gctgttttca tgtttttctc caatatatcc
    22501 agaatatatg tttgattctt cctattcaaa aaggccgaga cattcctatt tcaggaaatg
    22561 tcccagcccc caacctaatt ttaatgatta tgcagtttgt ttttctacgt aagtcactaa
    22621 atcttgaacc gttttgattc cgtcttctgt ttcgattttc acgtcaaatt catcttcaat
    22681 ttcattgatg atttggaata aatccaagct gtcagcttct aagtcttctt ggatgtcagt
    22741 agtcaatttg atttcttcag tttcttttcc taattcttct gcgatgattt cttgaatttt
    22801 ttcgaatacc ataataatat attcctccga tattgtttgc tttttatttt tataatttaa
    22861 cagtctacaa ctccatgaga atacttcccc atgttaaacc tccgccatag cctgtaaata
    22921 ttactttctg ctgttttcct aaggtgatct ttccactaga aactgcttca tccaataaga
    22981 tagggatgct tgcagcagat gtattcccat atttatccat attggtcagg aacttttctt
    23041 gcggcatctt tacttttcgg gcaattttct caataattct gaggtttgcc tgatgcaaaa
    23101 gcagataatc aactggtttg tctttcatga cttcttggat attgtttgca acgtctcgta
    23161 cggcaaaatc aaaaatatct ctgccagtca tttgcaaata atacgaacct tctgaatctt
    23221 ctgaatagaa cggattttta ttttgctggt agccagcggt caatgagcta cttctggtac
    23281 cgtcagctgc taatttttct ttcagcaatt ttttctgtgg attcgcttct aagagaatcc
    23341 cacctgctcc atcgccaaac aacacagcag tcgtacgatc agaccaatcg attagcttag
    23401 atagtgtttc accaccaatc accagtccct tagttgagcc gcagcggatc attttttcag
    23461 ccatgcttaa cgcataaaca aatcctgagc acgcagcact gatatcaaaa gcgatagcat
    23521 gagttgctcc caatttccct tgtacttgtg ctgctacaga tggtgtcgtg taatcgggcg
    23581 acattgttgc tactaaaata aaatccaaat ctttagggtt tagatttttc tgttttatca
    23641 atttttccgc cactaaaatg cataaatcag aagtctcctg atttgttaca catcttcgtt
    23701 cacgaatacc cgtccttgac gaaatccatt catcagaggt atccatcatc ttggaaagat
    23761 cattattagt aataatcttt tcaggaagga cgctggctgt tgcagtaatc attccaaatt
    23821 gttccattaa atatcctcac ttatagtctt gcaagaagtc gtgcaagttt ttcagtgcat
    23881 ccagcaaggc atgctgttcc tccgtagcca ttccgtctaa gatgttcttg accatttcac
    23941 gatgaaaatg ctgatggacc cgaaatagta actttccttt tttcgttagt cccaacttta
    24001 caacgcggcg gtcatcttca ctgcggatac gttcgacata gccttttttt actaacttgt
    24061 taatagcagt ggtcaaggtc ccaacagtaa ttgatagttc tttggctact tctgaagttg
    24121 tttttttctt gtacatcccg attgcctcga tcgtgtgcat ctctgtgatt gacagatctt
    24181 tgaattgaga ttttttcagc tcagactctt cgattgtcaa aatatcattg aagacactaa
    24241 caagataatc atttactgtt tttaaatctg gttccatgtt ccaccgtcca ctaattagtt
    24301 tgattatcaa atcttttgat gttcaaacta tatcttaact tttttcaaaa tgcaac
//
LOCUS       DACCXO010000034        23603 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.035, whole genome shotgun sequence.
ACCESSION   DACCXO010000034 DACCXO010000000
VERSION     DACCXO010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.035"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            46..1386
                     /locus_tag="IVY70_001262"
     CDS             46..1386
                     /locus_tag="IVY70_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7958399.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1604..2047
                     /locus_tag="IVY70_001263"
     CDS             1604..2047
                     /locus_tag="IVY70_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP7958400.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2551..3969)
                     /gene="nhaC"
                     /locus_tag="IVY70_001264"
     CDS             complement(2551..3969)
                     /gene="nhaC"
                     /locus_tag="IVY70_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP7958401.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4372..5595)
                     /locus_tag="IVY70_001265"
     CDS             complement(4372..5595)
                     /locus_tag="IVY70_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP7958402.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5626..6465)
                     /locus_tag="IVY70_001266"
     CDS             complement(5626..6465)
                     /locus_tag="IVY70_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP7958403.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6482..7162)
                     /locus_tag="IVY70_001267"
     CDS             complement(6482..7162)
                     /locus_tag="IVY70_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7958404.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7164..8237)
                     /locus_tag="IVY70_001268"
     CDS             complement(7164..8237)
                     /locus_tag="IVY70_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7958405.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(8884..9555)
                     /locus_tag="IVY70_001269"
     CDS             complement(8884..9555)
                     /locus_tag="IVY70_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7958406.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9642..12320)
                     /gene="mgtA"
                     /locus_tag="IVY70_001270"
     CDS             complement(9642..12320)
                     /gene="mgtA"
                     /locus_tag="IVY70_001270"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP7958407.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            12979..13416
                     /gene="ndk"
                     /locus_tag="IVY70_001271"
     CDS             12979..13416
                     /gene="ndk"
                     /locus_tag="IVY70_001271"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP7958408.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13438..14304)
                     /locus_tag="IVY70_001272"
     CDS             complement(13438..14304)
                     /locus_tag="IVY70_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7958409.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14464..16557)
                     /locus_tag="IVY70_001273"
     CDS             complement(14464..16557)
                     /locus_tag="IVY70_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP7958410.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(17306..17929)
                     /locus_tag="IVY70_001274"
     CDS             complement(17306..17929)
                     /locus_tag="IVY70_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958411.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(18088..19485)
                     /locus_tag="IVY70_001275"
     CDS             complement(18088..19485)
                     /locus_tag="IVY70_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7958412.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(19517..20488)
                     /locus_tag="IVY70_001276"
     CDS             complement(19517..20488)
                     /locus_tag="IVY70_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7958413.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            20862..21287
                     /locus_tag="IVY70_001277"
     CDS             20862..21287
                     /locus_tag="IVY70_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958414.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(21775..22476)
                     /locus_tag="IVY70_001278"
     CDS             complement(21775..22476)
                     /locus_tag="IVY70_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP7958415.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(22492..22932)
                     /locus_tag="IVY70_001279"
     CDS             complement(22492..22932)
                     /locus_tag="IVY70_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP7958416.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(22932..23507)
                     /locus_tag="IVY70_001280"
     CDS             complement(22932..23507)
                     /locus_tag="IVY70_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP7958417.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT         6789 a         4895 c         4063 g         7856 t
ORIGIN      
        1 cttaatataa attttgattg tgaatttaaa agaaagaggt catttatgga agataaaaaa
       61 ttgaaaaaac aacttagctc cagacatatc accatgttag ctttaggtgg agcaatcggt
      121 gcaggtctat ttaaaggaag tggcgaagcg attgggatcg ccggcccttc tgtattgatt
      181 gcttttttga ttggtgggct gattttatat atcgtgatga aaggtctggg aaaaatcgtt
      241 ttaagcggtg gcgacacgca tcacggatta tctggtttga tacgccctta tctaggtgct
      301 cattctgctg attttactga ttgggtctac tggtctatgt ggattatcaa tatcatcgct
      361 gaagctgttg cagctgcttc ctttttgcag ttgtggttcc cgcacattcc agcctgggta
      421 tttgttttta tgttggctgt tttaactacg atcatcaatc tttattcggt acggttattc
      481 gcagaaacag aatattggct ggcttttgct aaaatcagtg tcattatctt actgatcatt
      541 tttgctactt atcttgtcgg tcagcaaatg ttgggcacag gcgttttccc tacccttcag
      601 caattgactg accatggtgg gtttactcct catggtatga agggaatcat cagttctttg
      661 cttgtagtta tctattctta cggcggttct gaactgattg ctatcacggt aagcgaagca
      721 gatgatccta aacgtgcgat tcctaaagca atcaaaggtg tcatgggaag aatcatttcc
      781 ttttacatca ttcctttgtt tttgcttttg attctttttc cttggaatac tttagcaggc
      841 acgaatgtca gtccttttgt gatggtattt gaaaaaatga atatcccatt tgcagcggat
      901 atcgtcaatt ttgtgattgt ccttgctttg ttctcatcta tcaattcggg tgtgtatgca
      961 tcttcaagaa ttttgttctt ccgtttgaag gatcgaaaag gttcctctag aaaactagct
     1021 gttttaaaca agcatcaagt accgcaacgt gctgttttat tttgtaccag tgttctttat
     1081 cttggtgtag ctctttctta ttttgtcggt gacaaattat ttggctatct ggcaggttcc
     1141 ctttcgtata cagtattatt gatctggatc attatcagtg cggcatcctt tgtactggca
     1201 ctaagaaaag gaacattatg ggataaagga atcagctttt ttgcattagc tgtcttagga
     1261 ttgattttcc ttggtatcct gttcacgaac tccattgggg taactgtatt gactggcctg
     1321 ctttacctgt tcatttattt cagttatcaa aaaaagaatg atgcgttcgt attgacgaat
     1381 gaatgaaatc gatagaaaat tttattttat ttatgtggaa tagatggagc tattgaagaa
     1441 aggagttgtg acaaaagtct tgttgcaact cctttttccg aataaacagt ctcatgacct
     1501 cttggtaaac ggtgttcaaa aaccctctgt gcaaaataat tgattgttct ccatgctcat
     1561 gacatgatag aagaaaggaa tgaaaaaaag gagtttgttt taaatgaaaa agtattggaa
     1621 gtttttgttg atgatcggtg tttcaacggt agttatgttt ggtatgatgt acttgaatgt
     1681 ctatgcgctc gatcatatct tcttcagtcg tacacgagta ttcatggcgt tgatgatggg
     1741 tgcagtcatg gcagtcatca tgctgttgtt catgtggaaa atgtatgaaa acaaaaagat
     1801 aaatgttatg atcttagccg ctagtcttct tgtttttgcc ggttccttgt ttatggtacg
     1861 aagccaaact actgtcaacg acacagcttg gatgaaggcc atgattcctc atcattctat
     1921 cgctatcctg gcaagtaaac gagctgattt aaaagatcct gaagtaaaaa aattagcgga
     1981 agaaatcatt caggcacaag aagaagagat cgacaaaatg aaaaaaatga tcaaagaaat
     2041 ggaatgacca ttatccgtcc aaataaaaaa agaggatgtt ccagaagtta tcagcttcaa
     2101 gaaataagaa gaagctactt ctggaacgcc gtgaataagg ggttgtgaaa aagctgccct
     2161 gcatcaagta ataagaacag ccaaacaaaa atagcttctc ctatttttcg tttggttagg
     2221 cttattaccg cagatgcaag cttttgaaca ccgtttatta agaggttgta aaatcagcgt
     2281 tctgacctga gtattagaga gacaaatgga aaaatagttc ctcatatttt gccatttttt
     2341 gagctaatac cgagggtcag ctgattgaac accgtttatc gagaggttgt gaaaaagtcg
     2401 cttagtttcg cgtattaaga aaaattttgt gaaatggctt tccacatttt tgtataaatt
     2461 tggcttaata ccgagaaact ggcttttgaa taccgtttat tcgtaaaagg gattgtgaca
     2521 aaagtcttgt cacaatccct ttttatgttt ttattgttct aattgcgctg tcttgcttac
     2581 tgcttcttgc ggtttcacaa atttttttga gattccccaa cctgtgaagg cactgaccat
     2641 cgaaaacaca ggagaaagca aactgaaaaa tacgaatggc aagtaagtga gtgtaggaat
     2701 accaaatgta cttgctacga aacttccggc aattccccaa ggcactaagt agttgattac
     2761 tgttccacca tcttctagcg ttctacctaa tacagttgga tcaaatcctg ctttttcata
     2821 agtttcttta aatgcattac caggcagaat aacagatagg aattgttcac caataaaaat
     2881 atttactccg atacttgtca tcaatgttgt aaaaatcaaa tttggtgtgt tcgtcatctt
     2941 tcttgatact cgttccatca cggttgagat caagccaaat tcgatcaaca atccacctaa
     3001 tgacaaagtt agaataatca atgacacagt aggcatcata ctttcgattc ctccgcggct
     3061 aagcagtgaa tcgatttgtt ggtttccagt atttgaaaca aaccctgatt cgatgacccc
     3121 agccaacttc gttaaggaag tttgtgggtt ttgaataaaa atcattccta ctgctacagc
     3181 gatattcaac aaaatcgtta aaattgccgg cacctttttc catgcacaca gtaacattaa
     3241 aaataaagga atcagtgacc agattgaaat agtaaagttt gcttctagaa catcagtcac
     3301 ttcacgaata gatgaaagac ttgcggcttt acttgtgtgt cctaaaatca tgaataaaaa
     3361 gaaagaaaca acaaatgccg ggacagttga ccacatcata tgcttgatgt gtttgaacaa
     3421 atcggcatca acaatagctg ctgctagatt tgttgattcg gataatggag acatcttatc
     3481 tccaaaaacg gcacctgaaa caatagctcc aacaaccagc gctggattta agcctagtgt
     3541 tgtcccaata ccgaaaaatg caatcccaat cgttgacata acagtaaagg cactccctac
     3601 agcgctacct acgattgcac acacaacaaa tacagaaggg acaaaccact ggacactgat
     3661 caatttgaac ccaatgacca ttagagttgg aatgatccct gcttgtatcc atacagcaat
     3721 caatgaaccg actaaaataa aaataaagat tgggatgatt cctggtttga tgccattgat
     3781 gattccttcg tgcagccaat ctgtcgggta tctttttatc actgcataca acatgaccat
     3841 tccgatcgct gctaagatcg tgatattagg tgagaccttt aatccgacaa cgccaatcgc
     3901 aatgcctaaa acaataacca ttaatacaag tgttgcttct aaaaaacttg gtgttccttt
     3961 ttttcccatg ataatctctc cttttttagt tgttcttatg ttctaaaaaa gttaagatat
     4021 cggattactc cgccgcaatt catccgcgat tcctccttta taaaaataaa aagacctgtt
     4081 ttaaactaag gacgaatgac tccgcggtac caccttaatt aaaaacagat ctgtttttca
     4141 ctttcaaact tttattcaaa tgtatggact ttccatgtaa ttcgtcaatt aggcttgatc
     4201 ggttcacagc gaccaccgac tttctgaaca aaacacctaa ccgctactgc gtgaaaagtt
     4261 gacttgtacg aagtttacct tccataagat catttgtcaa tgaaaaagaa aatatttttt
     4321 catttttctg aaaattattt ttataacttt cacacacaag tttatttttt actagaaagt
     4381 agcgccctct tgtttttttc ttgctactat ttcttgatgt tcttgcttga ttctttctaa
     4441 tagctttggc tctttcaaca gatcaagagc tgtattggcg agtagttctg ctgcaatcgc
     4501 aatagaagca aggcctttgt cacttttagc agcagctttg aattcttctg tgtgtccggc
     4561 aatatatgtc tcagagattg ctaccgtagg ctggattgtt ggtatgactt ggctgacatt
     4621 tcctacatca gaagaaccga gacttgttct ttgttccgtt tctatttctt catctggaac
     4681 acctgcttct tttgcatgct ggaagaataa gtcatcaaaa gaaggagtaa cgatgacatc
     4741 atcaactgca ttttggaaca atccaaactc aaaagtcgtg cccgtactta tcgcagcacc
     4801 tcgtacgata ttttccactt tttcatatac tttatctaat gtttgccgat ttcctgcacg
     4861 taaatagaaa cgtccagcag cgtaatctgg aatgacgttg gcagctactc ctccgtctgt
     4921 aatgatccca tggatattga catcttttgg caattgcaag cgtagagcgt tgattccatt
     4981 aaacacttgg atcaatgctt ccaaagcatt tactcctttt tcaggtgctg ctgctgcatg
     5041 ggaagcgaca ccgtaaaatt tgatgtcgac tggatcattc gctaaagatt ctgtcgtttt
     5101 cccataacga aaagctggat ggacgcataa cgctacatcg acatcctcaa aaaaaccttc
     5161 tcgaacaaag ctgcctttag ctgatccgtt ctcgccccct tcttcccctg gggtgccata
     5221 tacacggatt tctccaccag tttcatcaat gaattgcttc actatgctgg cggctaatac
     5281 ggagtaagta ccaaacaaat tatgcccgca tgcatgacca atacctggta aagcatcgaa
     5341 ttctgccaaa aaggcaattg tgggaccggg tttattggaa atataacgtg catcaaaggc
     5401 tgttcggtgt ccagctactt ctttttctac ccaaaacctt tctttttcta attgtttaat
     5461 caacgctgct gaggaataaa actcatagtt acttacttca ggatgttcgt ggatatctag
     5521 agcaagtgct tgatattctg gtaaacgata ttggattaga tccttcaatt ggtttgttga
     5581 gattgttgtt gtcataaaaa attcctccta tgctagtcat ttttcttatt ttctcccgaa
     5641 tgtttcccat gcaggcacgc ttgatccttt tgatgttttc tcgattactt tgactgtgtc
     5701 ttctgtttga taagcgtcta ctattttttg atacgtttta ttgttttcat cttcttttct
     5761 tgcaacgatg atattcacat aaggacgtgc atggtcatcc actggttcta agaagatggc
     5821 atcagaagca ggattaaatc cagcatctac agctacaccg ctattgatca ctgaaatgtc
     5881 tacatctgac aaagcacgtg ctgtttggga agcatctaat tcagaaatgt ctagattttt
     5941 tctattctcg gtaatatcag aaacagtggg cgcttgtcct tttgccttat ccactttgat
     6001 caatccagca gtttgcaata acagaagagc tctgccgcca tttgtaacat cattagggat
     6061 tgctactttt gctccgtctt tgatatcagc tacatccttc aaagtgttag aataaattcc
     6121 cagcggtgca ttgacagtat ttccgatcga tactaaatca gttccgtgtt ctttattgta
     6181 attgtctaaa aagaattgat gttggaaaga attcaaatca atcgatccat ctgctaaagc
     6241 cacatttggt tgattgtaat ctgtaaattt cacgtattcc aaattaatgt tctccttttt
     6301 caaacggtct ttcacgttgt cccatacatc tgtatcttct ccaataatcc ctagttttac
     6361 gatagtttct tttttgtcag ttgtatctgc ttttccttgg ctacaccctg ccaaccaaat
     6421 aattgctaca aatgtaatga ctacaaaacg aataattttt ttcatttttt ctcctccact
     6481 ttcaatgtgt aattttttta atcagttgat tactgataaa ttgacttaaa aaaactaaga
     6541 ttagaatcaa taatgtagcc acaatcgtta catctgtttg gaatcgatta taccctctcg
     6601 atatagcaag attccctaat ccgcctgcac ctactgctcc agccatggta gtcagtccga
     6661 tcacgttgat gatagtcaac gccgagaccc gaatgatacc aggcaaacct tctaacaaat
     6721 acacacgtaa aatgataccg attggactgg ttcccatcgc ttctgccgct tcgatcaccc
     6781 caggatcaac ttctagcagt gcgttctcga tttgacgagc aaaaaacgga acaactccaa
     6841 aaatcaatgg cactaaagct gctttcgcac caatcgtcgt tccaactaag aaacgagtag
     6901 tcaatgctaa aagagaaagc aagataatga aagggatgga acgaatgata ttgatcagct
     6961 tatcaagtat ttcaaatagc agacggttag ctagtaatcc attagggcgt gtcactacta
     7021 acgtcacacc taagatgatt ccaatcatcc cgccaatgaa tgctgtccaa aaagacatat
     7081 atagtgtttg cttcgtcgct tcgacaaatt catccggaat caaataaaca ttgggcaaat
     7141 atgttttaaa gaaatcagtc attttatctt cctccttcga tcactttgat acgactatct
     7201 aaatcgtttt tttgtaaaat atttattttt acaccagatt tgatcaaata atccaatgct
     7261 tttttccgct gtttggtttc tccagctaaa gtaacgatca aactccctaa cggcgtttct
     7321 tgtaataact ctacattgcc atataaaata ttcgctgtta cttgatattt gctgtacaga
     7381 tgagcgatta gaggttcgtt cgtctgattt ccaatataac ttaattccac cagccattta
     7441 ttggcgatct gatcggcaaa agcagaatgg gcaatgatcg tttctaatgc ttggtcaaga
     7501 tgagtagctg ttcgaataaa atctttggtc aattcttctt caggattgct gaagatctgg
     7561 atgctgctac ctttttcaac gattcttcca tcttccataa ccgctacctt gttgcagatt
     7621 tctttgacaa cttgcatctc atgagtgatc aggacgattg ttaaacctaa ctggcgattc
     7681 aattttttta acaatgcaag gatctgaagg gtcgttttcg gatcaagtgc actggttgct
     7741 tcatcacata ataatacctt aggttcattc gccaaagctc gtgcgattgc tactcgttgt
     7801 ttttgtcccc ccgacagttg tttaggataa gcagatgctt tttcttccaa accgacaagt
     7861 tccagtagtt cattcacttt ttgacgacgt tcctgtttcg attttccaga atactttaaa
     7921 gaaaaatcaa cgttgtcaaa aatagttcgg ctttccatta gattgaaatg ctgaaagatc
     7981 ataccgatgg cctttctttc ttttcgcaat tctttgggag agagctgact cagattcgtc
     8041 tgattgacaa tgacttctcc tgctgttggt ttctgcagca aattcatcac acgaactaac
     8101 gtacttttcc ctgcacctga gtaaccgacg attccataaa catcgccttt ttcaatcgtt
     8161 aaatctacag aatctactgc ctgtatctct ttttcttttt gtttgaaaca tacagaaaca
     8221 tctcgcaatt caatcattaa aattcctcct tatctaaaaa aagcgtcctc aagaaaatga
     8281 aaatgtcatt ttcttgagga cgcttttttg cgtgttacca cctcatgttc aagctgtgtt
     8341 cacacacaac tcctcttcaa gtacgatcat actctagcaa gatatcggtc gcagccgtca
     8401 tcgccttaca tctatgctcg ggatgaattg ctcgatggcc attttccaat agtcgttcct
     8461 tgcttcttct cagctaccga agctctctgt caaaggttct tctatcgtac ttaccatttc
     8521 aatgcaaata atattcaaaa aaaaagtcct tttactaaca taaactatgt cagtaaaagg
     8581 acgacgtatc gcggtaccac cttttttcgg aagtttcctt ccctcaagcc caacatctca
     8641 aaaagagaga ttggcgttcg ttttaacggt cgaacagttc cgaatttgcc tacttatcga
     8701 caaattattc tccaaggcca ttttcagctt ttcttacttg actcttctca ccagccagag
     8761 cctctctgca aagtttccaa acttactttc cttttcaacg aaatcgatat tttgttgttg
     8821 ggattaatct acatgaggaa aactgaaaag tcaagtacga aattcaaatt attttttcta
     8881 tatttatttt tctgtttttt tatttaacat ttcaataaca gttcgaagat cttttccttc
     8941 cgcaagaagt ttcaattttt ccttttcttc gacggtataa tttcggacaa aactggaatc
     9001 aggtatcaaa acaacgccta atcctgctcg tgaagcagct gttgctccta ctaaagaatc
     9061 ttccactgca aaggctgaac tagcttctaa tcctgttcgt tccaaagctg tcagataagg
     9121 aatcggtgaa ggcttgttct ccgacacttc gtctccgaat gtaaacgtgt ccaagtaatc
     9181 agctaattta aagtgatcca agatatctgt tgctcttttc gtaacagtgg tcgtagcaat
     9241 gcctagtttc atatgacgtt cttttaagtc tgccaatatt tcttttgcat atggttttaa
     9301 gtggatggct ccgcttgtca gctgttgata aaaatattct tcacgcttcg ctcggacttc
     9361 ctgtactttt tcagggctac ctgctgcttt cactaaaata tcaaaacttt gtttccagtt
     9421 ttttccgccc caagcgtcaa cgatttcttt tgggatatgg atatcaaatt gctgaaatcc
     9481 ccaaagccag ccatcacgat acgttttttc tgtgtcaact aacaaaccat ctaaatcaaa
     9541 tattgcacct ttcatctata cattaatcct ttccaaaaca atacacatag tttagcacga
     9601 ctggtccaaa aagaacagaa gcaaagaacc agccttctcg tttataagag ttcttggaat
     9661 cgacggatat agattttctt cacagctgtc acaagcgcca gataagcgat gactgtagca
     9721 aataaccata accagtaatt gccaggaagt ggcatcaagc ctaattcagc accaaaactt
     9781 gtaaaaggaa ggacagaacc gacaataatt cctaatgtag tgataacaaa catggcaaat
     9841 gttgcgttgc tttggataaa tggtactttc ggtgtacgca gtgcatggag cactaaggtt
     9901 tgtgtccata gtgattcgac aaaccatcct gcatggaata atgcgatgaa aatcactcgc
     9961 tgtgaactat ccaatgtatg gaaacttccg cccactacag caggacaaat aacaaaatac
    10021 atcaacaagt aagtagtaat atcaaatatc gaacttgttg gaccaaagta aaccatgaat
    10081 tccccaatac tagatgcatt ccattttttt ggctcgtcca aatattcttt atccatccga
    10141 tcccatggca aggaaatgca ggaaacatca tagatcagat tcaaaaacaa aagttgtaaa
    10201 ggcagcattg gtaaaaatgg caggaacgta cttgcaatca gcacggaaaa catattgcca
    10261 aaattagaac ttgctgtagc cttgatatat ttcatgatat tcccaaaagt ttctcgtcca
    10321 gaaagaaggc ctctttccaa aatcgtcaaa tctttttcta aaaggatcac gtctgcagat
    10381 tctttcgcaa tatccacagc tgtatctaca gaaataccga tatcagccac tttcattgca
    10441 ggagcgtcat tgataccatc tcctaaaaat ccgacagtat gaccattttt acgtaaagct
    10501 gtcgtcaaac gtgctttttg ctgaggattc aattttacga atacttcatg gtcttcagct
    10561 acttgtgcta attcatgatc actcatttcc gagattttct caccggtaac caattcattg
    10621 atctctagac ctacttgacg gcacactgaa cgtgtaacta attcgttgtc cccagtcaga
    10681 acttttacag ctacaccatg ttctttcaga gctttcaacg cttgtttcgt tgtttctttt
    10741 ggtggatcaa ggaaagcaag ataaccaatc aaaaccatgt ctgactcatc tttgactgaa
    10801 aattctccaa cgacgcttgg attggtcttt tgtgcgacac caatcactcg taagccatct
    10861 tcattaagtg actgtacttt tgcaagtacg gcttcttttc gttcttttgt taatggtgta
    10921 acagtaccgc caagatcaat aaatttagaa atgttcagca tttcttcaat cgcgcctttt
    10981 gtgatcattt gtgttttacc agctggatca gcaaccacaa cactcatacg acgacgttca
    11041 aaatcaaaag gaatttcatc cactttttga taattgatct tatctgtatc taaagtttct
    11101 gttgcttcat caatgatagc cacatctaac aaatttttca gccctgtttg atagtagcta
    11161 tttagatacg catggcgcaa aacacgatca tcttcatttc cttcacaatc catatgatat
    11221 tcaagaatga ttttatcttg tgtcaatgtc cccgttttat ccgtacataa cacatcgatt
    11281 gcaccaaagt tctggatggc attcaaattt ttgatcactg ttcctttttt ggccatcaca
    11341 cttgctcctt tgaccaaatt cgtcgtaacg atcattggaa gcatttcagg tgttagacca
    11401 actgctacag atagtccaaa aagaaaagct tctagccaat ccccttttgt aagcccattg
    11461 atgacgataa ctgctggagc catcaatgac ataaatttga tcagtagcat ggatgtttta
    11521 tggatgccta attcaaagct tgattgaatt ggtttttcag atagcgtttc tgctacatga
    11581 ccaaatagtg tttcatttcc aacagaaaca atgattcctt ccgcacttcc actgatgaca
    11641 ttacttccca taaagacaag attttcgtaa cttgtttcgc tttgctgatc gtcttcacat
    11701 actttcgcat gcttttctac tggatagctt tcccctgtca atgctgcttg ggaaatgaat
    11761 aaatcctttg cggacaataa acgcatatcc gctggtatca tatcccctgc tgaaagccga
    11821 acaagatctc cacagacgat ttcttccata ggaatctctg tgtactttcc attacgtttt
    11881 acagctgccg ttactttcac aagtgatttc agtttttcag ccgcttgatt ggaacgtacg
    11941 gactgaatca acgtcattgt cccgctgacg ataaccatag tgaagataat ggctacaccg
    12001 aaaagatctt tatctccagg agctgctaat acatattccg tgatgaatga aataattcct
    12061 aatgcgatca acactaatgt aaatggcgtg atgtatgctt tcaatatttc tacaatgaat
    12121 ggtgtttttt tgccatatga aatcgcattt tcaccgtaat tttctcttga acgttcgatt
    12181 tgttcttctg tcaatccatt tgctgatgca tggaatagtt caaataattc ttgtttcgtt
    12241 tttccagcaa aagctgcata gattttttgt gtatctttct ttttttccat ctcaatctct
    12301 cctttgtgtc caaaagacag ctgttctcgc tgcttttggt ctttagctat tcatcggatc
    12361 gatagagaaa aaactgaaag gggctgagaa cagatagata atagccctgg cctttcctct
    12421 agatccttgt ttttcgaatg actgtcatcc atttgttctc atctccttta ctaagtaatt
    12481 ttaattcatg acttccaatc atttttcgaa tagttatttt tccaattaga ccacctcctt
    12541 tttatttttc accgatgggg atttgcggca acaaaaaaac ctctcgccaa atactcagcg
    12601 aaaggtttga aaaagcatat taaaaaaagc ttctcagatc tcatcgttga gttttgacac
    12661 tgtacaacgt aaaaagcaga tacctgcttt tagctacctt atgaaaagcc ttatctcgac
    12721 aattcctgtt ctacccattg gcgtctctcg acgtttttgg gcagtagcct atgtttgtat
    12781 aggagcctca cctaacaaat cttattcggt tttccttcgt taatataaca actaaaaaag
    12841 aggatgtcaa atcaattttc catgtttttc atactttttt taaaatacac acgttatatt
    12901 cctgtctcac gcggttttat ttgtctcatt ttccacctcc cgctataata gaaatatagt
    12961 aggaaacgag gtgctgttat ggaaagaaca cttgttatta tcaagccaga cggcgtgaga
    13021 cgccatttag tcggaagcat catccaacgt tttgaagcaa agggactggc cattgctgaa
    13081 atgaagtttg aaactatgac tcctgaatta gcaaaagaac attatcagca cttgactgag
    13141 cgcagttttt ttgatgaact gatcgattac atgacttccg gtcctgtcgt ttatcttgtg
    13201 ctggtcggcg aggaagtgat cgatatcgtc cgtaagatgg ttggagcaac aaaagctgcc
    13261 gatgctgttc caggtacgat ccgaggagac tacgcgctgc cggggactga aaacatcatt
    13321 cacgcttctg attcccgtga tgctgcggta aaagagatcg cccgcttttt cccgccattc
    13381 gatataaaca aaccgtctga aaaagcggcg aagtgataca aattctaaac tgctggtcta
    13441 gttgctgggt tcagcaatgg ccagcagttt tttctctaag gacctccgtt gattctttcc
    13501 taacatatat tcgagcactg tgaagaattc ttggtagtct ttcgtctttt cttcccctaa
    13561 agcagcagaa atcaatcttt gataataatc tgccatcaat gtttcataga tcactgtact
    13621 tctttcttta tgtgataagc tccgtttcat atcttcattc acactacgtg cctttttcaa
    13681 ccgaccattt tctatataaa acgaagtcag gttcattaaa aaagtaaaga gatactgctc
    13741 atgattgttc aactgtgcta atcgtcgcat tctttttttg attttcaaat aattgttctc
    13801 aatgaaccga tcactgaata tccaaggaaa atcagctaga atagatagtt caaattgacc
    13861 atagtctggt atctgcatca agtattcttc aatgattcgg cattcataac gtaaaaattc
    13921 aaattctgtc tcagatactt ctgttttttc gaccatgtga agattcaaaa ccaaataata
    13981 cagatcttct gttttcttaa aatgcctacg gttattgctg atctctttat aaggatcaaa
    14041 gctgtcttct tgtacccgat ctagcattcg tctttttttc tttaacttta ttggttcatc
    14101 cagctggttt cgcatccatt catattctgc aagtgtcatg ttcattcgct ctaaaaatga
    14161 tctcaatgtt acaaatgaca tttttttcac tgaatttgtc tcaatcccga ctagagaagt
    14221 cttaaaacag attccttccg ctaattggga ctgtgtcaac ttcttgttga ctcgaagttt
    14281 cttgatcaat tcaccatcat acataatagt tctccttttt aatttaaatt tttccacatt
    14341 caataaatag atacgttaaa aaggaaaaat acacgaaaaa aataaaaaaa tgtttttttc
    14401 ataaaaaata ccaaccttca agcattagaa tgttgtctaa ctactgaagg ttggtttgat
    14461 tgtttattga ttattttttt gctgttcaac gtctgttctt gttactggat ataagttggt
    14521 tgttgtttta ttaccatttt ttgaaaagac acttgtcgaa tgttcgcctt tttgcttttc
    14581 gattgcttca ttacgttccg cttcattttt atagtcatat tttttcggat caatcgtctc
    14641 aaatccgttt ggcacataaa accgcaagag attttcctga ttcagcttat ctgaaagact
    14701 taacgcagtc ttgacttttt cctgatcttt ggagatttcc tcatcgattc ctgctgcttt
    14761 cgtatcgatg atttctcctg tctgattatt gtaagctttt cctcctagga cggtgtattt
    14821 gggtgtcaca aagttcccat ttcgaaaagc aactacttga tcatgctgag aagataataa
    14881 gtctgtacca aaatgaagat aatctttatc atcgattccc aatagatgta gtaatgtcgg
    14941 caaaacatcg atttctccac cgtattcatg attgactgtc cctttagtat aaccaggcat
    15001 atggatcatc aatggcactc gttgcatctg tgcgttgtca aattcatccc atgtatctgg
    15061 atctttacca agagcgcttg ctaaatcttt attatctgta ttcgagatcc caaaatgatc
    15121 cccgtaaatc acaaagattg aattttggta aataccagat tttttcaaat acgtaaaaaa
    15181 ttgttccaat gattgatcca gataatgtgc cgttcgaaca taattatcta ctgtgctatt
    15241 gccagtattc aaagatggaa agtcgaaatt cttgtcatcc gtataataag gcgtgtggtt
    15301 ggttacggaa agaaatttcg tgtaaaacgg ctgttgtaga tgctcaagat attgggcaga
    15361 ttctctgaat aaatctttat ctaatatccc ataacctaac gtttcgtcgg attcatcaaa
    15421 ataagatcga tcaaagaaat tgtcataacc taagttttta tatacatgat ctctattcca
    15481 aaaactagct acattcccat ggaaaacagc gcttgtatag ccttgctttt gtccaaggat
    15541 cgctggtgcg gcttgaaaga cattgtctga cccaagttct gtgaataatg agccttgagg
    15601 taaaccaaat gtgccagttt ccaacatatt ttcagcatca cttgtctttc cctgtcctac
    15661 ttcatgaaag aaattgtcaa agctgatcgt tgcttgattc tggaaaatac tatttaaaaa
    15721 cggtgtgact tcttgtccgt ctaccttcat attaatcaaa aactgctgga aactttctaa
    15781 atggagaacg atcacatttt tccctttagc gatcccaaat gtttcgggat ttggctctgc
    15841 ataatgtttc tttgtataat ctaaaacttc atcaatccca ttactgcttg catgcgcgcg
    15901 aacactgcta gtcattcctg ttttgattcc gtcataaata gtatacgcat cgattcctag
    15961 atatttgaca atatatgaac ggtcaaatgt tcgctgcagc aattgtggac ggttggcttc
    16021 gctcagcgct agatttcctg aaaaaataag ccctgaaaga caggtaacag cgatagccat
    16081 cggtttgccg acagggttgc ttttgatagg tattttcttg accttcaacc aaaccagtag
    16141 cccaatgaac acagcgatat ccagccagta gatgacatca tggggcttca tcaaagaaaa
    16201 ggagcttcct gacaatcctt gcgacacttt cgaaaagcct aatacagatt tgactgtaat
    16261 gaaatctgtg aattcccgat aaaaaataat attcaagtac aacaaaagcg tatttaaaat
    16321 atcaatgatc aaaagaatag ctaacgctgg ctttggcttt ttaatataca aagaaaggcc
    16381 aaaaaacaat aaggtagtcg caattgggtt gatccacaag ataaagtact ggatcgtacc
    16441 ttccacacct aatgaaaaat ctgcatagta ggcaatgatc gtcttcagcc agaaaaagaa
    16501 aacagctgtc aaaacgattg ctaaacgatt ttggcacaat tttttaagtt gtgtcacgta
    16561 tatcacttct ctctattcac attttttctt attttgctcc tgaaaaatga aaatttccta
    16621 aaaaagttgt tatcttaaga tttttttatt ctttctaaag attattccgt ccacttttca
    16681 cagcataaaa ccgctgcgat gtaagcgatt aaactaatga agagataaaa gtcttgtggt
    16741 gaccgcttca ctccacggta ttcaaaagcc agtttctcgg tattcgtaca aaaaaagagg
    16801 ctgtgacaaa attcttgtcg cagctcctta tttcttgatg caggacagct ttttcacaac
    16861 ttcttcctta aacggtgttc tcctgccaac tcctcggcca caagtcacaa tatttataaa
    16921 acatgagaag ctgttttttg aaattgttcc ttgtggctca gagttaaacg gcaggctcaa
    16981 gacctcttga tacacggtgt tcaatcagct gaccctcggt attagattat gtcaggacgc
    17041 taattttaca acctcttggt aaacggtgtt caaaagcttg catctgcggt aataagccca
    17101 accaaacgaa aaatataagg agctattttt tgaaattgct ccttgtggct cagagttaaa
    17161 cggcaggctc aagacctctt gatacacggt gttcaatcag ctgaccttcg gtattagctc
    17221 aaaaaacggc aaaatatgag gaactatttt gccgtttgtt tctctaatac tcaggtcaaa
    17281 acgctgattt cacaacctct gtttttcatt gtccatagga ttgatattgt ccttgtgatg
    17341 actcattcgt aataattgag gcttgatttt cttcacttcc cgttatcgtt tgtccactgc
    17401 cagaatctga aggacttgtt cctgtcgact cgtttttttg agaagagaca gttgttttct
    17461 tgacttgttc atttgtcttt ttccattccg ctaaacgatc ggcgaccact tttttgtcta
    17521 ctttttcaga ataagcggtg atcacttttc catttttcaa acgggctact ttgtcgccat
    17581 catgagtcaa aagcaaaacg gtcaaatgct tgtctttaaa ttgatattgt ctgagagaaa
    17641 tcgcactaag tacgtcatcg atattaataa attgattttc ttcttttcct tttggcaaat
    17701 aaatgtccaa ttcgcctttt tcgtaattgg cagttatttt atactccgcc caccaataag
    17761 ttgagaattc ttttttgaaa ttatctactg attctttgct tgctacgatt ttttctgttt
    17821 ctttctgagc aagatctgct gtcatttttc ctgaaaatgc tgcggataaa ataagcagta
    17881 agaaaaaacc gaataaccaa aagagaagaa acggtctttt tttcctcatg ctttcctcca
    17941 ctcctattta attttgacta ttctattata tacaaaaaat gaaatagaaa gacaaaaaag
    18001 ttgaaaaaga tcgtaaataa ttgaacttct ttttcacaaa atggtaagaa tggagaattt
    18061 cacgcatttg ctctcaacta ttctttctta agcctcaaat ccgtttttat ctgatacgtc
    18121 tttgaaccaa tgacccgatt ttttgattgt tctttttcca tcttgtgcta agtctactga
    18181 aataaagcca tatcgattct tatatgcatt ggtccacgac cagttatcca tacatgtcca
    18241 catatgatag cctaaacaat tggcaccttc ttgaatggct tgatggacat atttcagatg
    18301 agattggaca aattcgatcc gataatcatc gtggatcatg ccatcttctt tgataaatcg
    18361 tgcttcgttt tctacaccca taccgttttc agaaataaag cagtcgatat ttccataatg
    18421 atctcgtaca ttgatcaaaa tatcatagat tccttgttca tagatctccc atccgcgata
    18481 aggattcatt ttacgaccag gcatttcata attgtcgaaa taatcctcag gcattggacc
    18541 gtttttagta tcgattggtg tttcttttgc ttttacccgg cgtggctggt aataattgat
    18601 acctaaaaga tctactgtat tttctttgat aaccgtcaaa tcctctgcat ggatcacagg
    18661 catgagatca agttctttta ctatctcaat caattcactt ggaaactcgc cttttacagc
    18721 tggatcaagg aatgaacgat tgaagaacgc atctgctatt tgcgctgctt taacatcttc
    18781 agggtttgat tcgtctcttg gatagcttgg tgtcagattc aagatgatac caataacacc
    18841 accttgctcc atttcatggt aagatttgat ggctaacgct gaagccaacg cttcatgata
    18901 gccaacttgg atcgcttttt tcatatctga ctcttctgga taatgccatc gatataaata
    18961 acctgcttcg actggaacga ttggttcatt atgagtaaac cattttttta ctttcgaacc
    19021 aaacaattca aaacaacttt tggcatattt ttcataggct tcaaccgttt ctcggttcaa
    19081 ccatccgcct ttttcttgca gatacatcgg catatcgaaa tgatacagat tcacaaaggg
    19141 ttcgataccg ttttcaatca actcatcaaa ataacgatga tagaaacgaa cggcttcttc
    19201 gttgattgtt ccagattcat caggtatcaa acggctccat tggatagatg ttcgaaatga
    19261 attatgccca gtctgtttca tcaactgtac atcctcttga tacttcgtat aaacttgcga
    19321 ggttttttca ggtcctactt gatcaaagaa cttttgcggt tcttttttgt accaataatc
    19381 aaaaatgttc tctccttttc catcgcctga aatccttcct tctgtttgtg ggccacttgc
    19441 tgcagaaccc caccaaaaat ttgctggaaa ttgatatttt ttcatcaaag cacctctttt
    19501 tcttttttag ttcgttttag ttgattccat gtatgatatt ttccatcaca atcacttttt
    19561 cattttcttc ctcttctttt ttgattcgtt ttaacagctg ttctgctgct cgttccccga
    19621 ttttatctgt tggtggcgtg atcgtgattt tcggaaggat actcagatcg tttttatcta
    19681 aataatcata gtgactgaac gcaatatcct tcccgattct gacaccttct tgattgaaac
    19741 attcgatggc accaatcgac atattgtaat tgctgacaaa aatagcagtt gccccttttt
    19801 ctaataatgc ttttgccccg taatagccgc tttttctaga atagtctcct gtaaagacct
    19861 ctaattggat cgatggatca ttttggaccg cttctttggc tcctgctaaa cgttctcttg
    19921 ctacgtagtc tgtttgagtg gcagcaataa atccgatttt tttatgtcct tgttcaccat
    19981 gatgagcgat cacttttgct acactttctc gatcattcac taatattgta tcaatacctg
    20041 ctggtccttc tggacaattc aatgcgataa ctggaatact catttttgtg agcatttcaa
    20101 atccagacca ctcttcttcg gataagaaga caatcagccc atcgatcaca tgcttcatca
    20161 ggtattcaat tttttgttca attttttctg gctgatcggc aaacccgctt aataataagc
    20221 tatacccatt ttcttcagct actctttcga tccctgagac aacttccgac ccaaaacggc
    20281 tggaaatccc gtttatcaaa agtccaatag agaagcttcg attattcttc aaccctttag
    20341 caatgatgtt ttctttatag tccaacgctg aaatcgcttc tgctattttt ttcatgttct
    20401 tttccttcag ttgctgacca ttcaagtaac gggaaactgt tcccactgaa acaccagcca
    20461 tcttagccac atcacggatc gatgccatac attctcactc ctgccagtta catttgattt
    20521 ctctttcttg ttgttcgatt atgatgaccg cattctttgc atagtgttgc ttgtcatcaa
    20581 tatccatata actaaagtct tcagtagagc aacttcctct cactattaat tatacttaaa
    20641 caagtatttg aaccggttca tttttaatat agcatttttt taaaagattg caacactttt
    20701 ttaaaacgct ttctgaaagt ggcggatttt ttgccttttt aaaattttat tctaaaattt
    20761 actctatttg tgggtgtttt gtgcgttttt ctcttccatc cgtttgaagt tttacattcc
    20821 aatggtataa tttctttatc ctatcaaatg ggggaagtat tttgaagaaa ttaatcgtac
    20881 tatttatttt gtctgcggga cttttgacat tgactggctg tgatttgttt ggcggctctg
    20941 accaatcctc ggacgaaaca accgattctt ctgtcttaaa agcggaaaaa gcagatacca
    21001 ccaacagtgt atatacgcta aaagtacgta aagatatgga tttatccgcg ggacagttga
    21061 catttacaga taaaaaaatg gaatggacac gcacgtatca gacacaagga actgattctt
    21121 ctgaatcatc tgaagaaaaa acagtgctga cagatatcaa gatcactacc aaagatgacg
    21181 aatatattat ttcaggaaaa gaaaataaaa aagaaacaac agtcatattt acaaaaatcg
    21241 gtaattaccg tttgaaggat gaggatggca acatctacag tctatagtgc cgtctgttgg
    21301 atcttctttt gtttctcccc ttcttttctg ctatagtaaa gaagcaagat gattgtaaag
    21361 gagacgaaat ggatgatcga tagtcctgat tattgtacat gtaataacaa caaaaatatc
    21421 catacagaat ttaatgacca aggaagcttg agcatatgcg acaactgtca caaactagtc
    21481 aaagattcat ttaaaacatt tgaatcttat cctgttcaaa attatgtaaa aagtagaaga
    21541 agataaacaa atccaagcaa aactcactat ggctgatttt tatgaacttc aaactcagct
    21601 tcttcctttg atcaagcttc atcagtaaaa caaacagtgg atcatttcag tttacaaatc
    21661 aaagagtaaa gatttattta tttcaaatat ttgaatataa aataaccaaa cgatagaaag
    21721 gcttattcta catcacgtag taatttttct ttcccgtcgt ttggtttttt atttttagcc
    21781 taattcttga ccttttgcat gctggttatc cgtaaaatat tttccagcag gctttccttc
    21841 caattcagct aatttttctt ggatctgacg gatcaccttt tttgcatctt ctggattttc
    21901 tacaaaatcg aatttatccc catccacttg tattttagga catacatcaa aatcttcata
    21961 ccattgatta tagcgggtgt tcaacgtttc ataataggta tataacgtct catcgctttc
    22021 tagctgttca tagtcacgcc cacgtttttt gatacgttct agcatcgtgt ccaaagaaac
    22081 tttgatatgg accatcaaat caggccgttt ttttggagca acatcatcta actcgtttag
    22141 catcgtatct aataagctat cgtactgctg tacttcgata tccgttaccc gacctaaatc
    22201 agcgtttaga tggaacaaca ggctatcttc atagatggat cggtctagca cattgtcatc
    22261 gttcactaaa gcatttttca ttgctaaaaa tcgcttgttc aaaaagaaga tttgcaataa
    22321 aaaagcgtac tgctcaggat ttgaataaaa gagcggcaat acttcgttat cttcgacatt
    22381 ttcataaaat ggtttagaat caatctcttt tgcaagcata tccgtcaaac tgctcttacc
    22441 tgcaccaatc gttcctgaaa tcaaaatcac tgacatacaa cccttcctct tctataagta
    22501 ctcgcctacg taagtttcaa caggcaatgt ttcaaaatca tattcacgta cagcttggtc
    22561 tgattttaaa tacgtatgga gactttgtgt aatcatcaag ttcgtaggta ccgtctcttc
    22621 ttgtacaatg atcatcggtt ttttcaacat agttgccaca cctaattcgt aagccgttcc
    22681 tggatcgatt gtttgatgct caaagtcaag gatagcaaca acaaattctg catttgtcag
    22741 ttcttccata tctttttcgt atacttcctg cgcccatcca gcgctgaata gttcataatt
    22801 gctttcttgg tgatgacgtg gagagtagaa acttgtgact gttttgtttt cctccaacgc
    22861 tttttcgata cgacttactc gatcgatttg ctcctctgaa aaaaatggtc ctgctaaata
    22921 tatttgactc attattttgc ctccttcaat aataactgat ttttaataga tactgctcgt
    22981 aattcattga ccagttccaa tggattgttg gaaaagatta cttgatccaa ccatttttta
    23041 tccataaaat cctcttttgc aaaagtttct aataattgtt gcaagccgct gtaataaccg
    23101 ccaatattca atattccgat tggcttgtcg ggcgtcagac cgattgctat ccaagaaaga
    23161 agttgtgaga attcctccaa tgtacctaga cctccaggga aaacgaggac agcatctccc
    23221 ttttcaaata ataatacctt tctttcatcg atcgtttctg taggaataaa tgtagtaaca
    23281 tcttttccag gcaattcagt ttcaaaaagc ccattcggat agatacctgt gacttttccg
    23341 ttattttcca gtactgtttt actaatcact cccataatcc cggagtatga accaccgtaa
    23401 accagttcga tttcttcttg tgccatgtat tttcctaacg tttgcgcaat aaatttgtaa
    23461 ctttctttat taccaaaacg ggaaccgcaa aaaacagtaa gtttcataaa aaaacactcc
    23521 ctaactcaaa agtctatctt cattctacac gtgttttttt aaaataaaag actagatttt
    23581 gtagtttttt agtggtatat agg
//
LOCUS       DACCXO010000035        23202 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.036, whole genome shotgun sequence.
ACCESSION   DACCXO010000035 DACCXO010000000
VERSION     DACCXO010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.036"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..655
                     /locus_tag="IVY70_001281"
     CDS             <1..655
                     /locus_tag="IVY70_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide excision repair endonuclease"
                     /protein_id="HAP7958418.1"
                     /translation="NKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEIS
                     LLALRVQLPDLQEMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQ
                     KDIELVTYFIRRIQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDV
                     SPDFYYEKKEAHLSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(712..942)
                     /locus_tag="IVY70_001282"
     CDS             complement(712..942)
                     /locus_tag="IVY70_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP7958419.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(1028..1915)
                     /locus_tag="IVY70_001283"
     CDS             complement(1028..1915)
                     /locus_tag="IVY70_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP7958420.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(2004..3227)
                     /locus_tag="IVY70_001284"
     CDS             complement(2004..3227)
                     /locus_tag="IVY70_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7958421.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(3244..4323)
                     /locus_tag="IVY70_001285"
     CDS             complement(3244..4323)
                     /locus_tag="IVY70_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7958422.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(4454..6181)
                     /gene="ptsP"
                     /locus_tag="IVY70_001286"
     CDS             complement(4454..6181)
                     /gene="ptsP"
                     /locus_tag="IVY70_001286"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP7958423.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(6181..6447)
                     /locus_tag="IVY70_001287"
     CDS             complement(6181..6447)
                     /locus_tag="IVY70_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP7958424.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            6926..9160
                     /locus_tag="IVY70_001288"
     CDS             6926..9160
                     /locus_tag="IVY70_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP7958425.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            9308..9628
                     /locus_tag="IVY70_001289"
     CDS             9308..9628
                     /locus_tag="IVY70_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP7958426.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(9833..10057)
                     /locus_tag="IVY70_001290"
     CDS             complement(9833..10057)
                     /locus_tag="IVY70_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAP7958427.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(10255..11835)
                     /locus_tag="IVY70_001291"
     CDS             complement(10255..11835)
                     /locus_tag="IVY70_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP7958428.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(12236..13612)
                     /locus_tag="IVY70_001292"
     CDS             complement(12236..13612)
                     /locus_tag="IVY70_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP7958429.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(13740..14858)
                     /locus_tag="IVY70_001293"
     CDS             complement(13740..14858)
                     /locus_tag="IVY70_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7958430.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            15000..15434
                     /locus_tag="IVY70_001294"
     CDS             15000..15434
                     /locus_tag="IVY70_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7958431.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(15512..16045)
                     /locus_tag="IVY70_001295"
     CDS             complement(15512..16045)
                     /locus_tag="IVY70_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP7958432.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(16139..17317)
                     /gene="mutY"
                     /locus_tag="IVY70_001296"
     CDS             complement(16139..17317)
                     /gene="mutY"
                     /locus_tag="IVY70_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP7958433.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(17310..18107)
                     /gene="recX"
                     /locus_tag="IVY70_001297"
     CDS             complement(17310..18107)
                     /gene="recX"
                     /locus_tag="IVY70_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP7958434.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            18214..19596
                     /gene="rlmD"
                     /locus_tag="IVY70_001298"
     CDS             18214..19596
                     /gene="rlmD"
                     /locus_tag="IVY70_001298"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP7958435.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(19770..20348)
                     /locus_tag="IVY70_001299"
     CDS             complement(19770..20348)
                     /locus_tag="IVY70_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP7958436.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            20670..21140
                     /locus_tag="IVY70_001300"
     CDS             20670..21140
                     /locus_tag="IVY70_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP7958437.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(21248..22474)
                     /locus_tag="IVY70_001301"
     CDS             complement(21248..22474)
                     /locus_tag="IVY70_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP7958438.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         6887 a         4701 c         3971 g         7643 t
ORIGIN      
        1 caataaggga tttgtattaa cagatactcc tacggctcga acatacgggc cttttcgtct
       61 ctataaaaaa atgccgtcta ttttgcgtat aatggaagaa acttatcaaa tgccttggtt
      121 gtctgaaatt agtttgttag ctttgcgagt ccagcttcct gacctacagg aaatgacttt
      181 tgaacagaaa aaaaaggaac tccaagggct gtttcaaggc cgaaataaaa agctactgac
      241 ttacttgaaa aaaagacaac aacatttcat ctatcagttg aatttcgaaa aagccggtat
      301 gcttcaaaaa gatatcgaat tagttactta ttttattaga cggatccagg aacaaaagca
      361 gtttttaaga acaccatcgc tcaccttcag catgccatta gcagcagatg agagtcagaa
      421 aaagcactac ctcgtttgtt atggtcaact tgcagaaaca atgattacct ctggtgacgt
      481 ttctcctgac ttctattatg aaaagaaaga agctcattta tctttgaaac gtcaattgtc
      541 aaaagaagaa atcgatcctg tacaaatctt gatcagttac cgcaaaaaat tagaaaaaga
      601 acagtttgaa attgaactgt taaacaaaaa agaagctgaa aagcaactaa attagttgct
      661 tctcggcttc ttttaggcat cttcaacttc aaatgtcaat tatcctaaaa tttattggat
      721 taagtcatag atttccatcg ctacgatatc gatattatca aattcgtatt gctgatgagt
      781 atctgtttca gttaattcaa atgtatcggt cgctttatca taagttactg tacacttctc
      841 tacgccttct ttttcaaaac ggcgaacttg cagttcatta tctgctgact cgaccatagc
      901 ctccagtcgt tttatgattg cgactaattg tgaatgtgtc atcggaggtc ctccttttct
      961 tatttctgtt atattgtatc aaaagcatag cctacttgca ttgattttct ctaaaaaaac
     1021 tcgtttttta tgattttttt tctggtattt ttccttcagg ccaccaaagt ttgatcaaag
     1081 cttgggttcc gtcattctca aaacctttat ctgccaactc ttcatatagt ttcaatgctt
     1141 gggtagttgc tggcaataca agatccattt tttctgcttc ttctaatgca atctttaaat
     1201 ctttgataaa atgtttgaca aaaaaaccag gtgtgtaatc ttcttttaaa atacgaggag
     1261 aataattgct taaagaccag ttagctgcac ttccacctga aagcgtctca atcactttct
     1321 ttaaatcaag tccgtttttt tgtgcataga caagcatttc cgtcattcct gtcatcgtac
     1381 ctgcaatcat gatctgatta gccatttttg tatgctgacc tttcccagca gaaccatgca
     1441 atgtaaatgt cgtaccaaat tctttgaata aaggcagtac ttgatcataa acggcttctt
     1501 ctccaccgac catgatcgtc agtgtaccat tctttgctcc taagtcacca ccggataccg
     1561 gtgcatctaa cgcatctgcg cctttttctt ttgctgtttt tgcaattttt tctgccaggc
     1621 ttggtgtact tgtagtcaaa tcaactaaaa tatgtcctga gatatctgca gaaaaaatcc
     1681 ccgactgtcc atagtagatt tcttctacat ctttggggta acctaccatt gtaaagagaa
     1741 tattactttg ttctgctacc gcttttggtg tatctgccca gatagctcct tctgttacta
     1801 aaggatcagc ttttgctttt gtccgattat agacagctac ttcgtgtcct gcttctagta
     1861 gatggcgtgc gacagcactt cccatgacgc ctgttccgat aaaaccaagt ttcatttttt
     1921 cgacctccta aaaatcaaaa atccttgctt ctatgctagt atacgctaga aacaaggatt
     1981 ttttcataaa aaatgtttga attttatctt ttaaatgaat ttaattttat tttgattcgt
     2041 tcaatcgagt cttcttcttc ttttcttcgc atctcttctt catgataaac aaccatgtct
     2101 tgataaaatt cgatcatagc ttcgccgaaa tgtgtagatg aaatatcata aagtttagct
     2161 tctaaaactt ttggatcttt aggaacttca ctggaaatat aatcaataag tgctggtgca
     2221 aagtcttcgt cggtttcaaa agttttccct aatgaaggat gatcgaacaa tcggttcaaa
     2281 taagcatttc cttccacgac acaaggtgtt ccggctgcca tcgcttctgt ataagtaagc
     2341 ccttgtgttt ctgaagtaga agcactgaca aaataatctg ccgctttata ataaagtgct
     2401 acatcattgt tgtccacttc tcctgtgaac tgcacaaatt cttctaactg cagttctgag
     2461 atcaattcct ctaactcttc tacataaggt ccttttccta cgataactaa tctggcgtta
     2521 ggataagcgg agatgacctg aggaaaccca ttaatcaagg cttggatatt tttctcataa
     2581 gaaatcctgc ttaaagaaag aatcatcaaa tggttttcct gaagacctag tgactccctt
     2641 aatttgctga tatcctgctg agtgatatca gaacgtttga atttttcaat gtcgatacca
     2701 gtaggaatca cgcgaagcgg tgctattaca ccatagctgc gtaatgtatc aataacacgt
     2761 tcacttggac aaactacacc tgtcgtatga tttgcaaata atctagacaa gtattttaca
     2821 tgggattgcc tgatcacttt tcctttaccg atataatgaa ggtagtcttc atacatcgta
     2881 tggtaagtat ggatcagagg tattttcaac ttcttagcta ccatcttccc aagtatccct
     2941 gctccgaatt ctgtatgggt atgaataaga tctagttcta gttctttggc aaccaaataa
     3001 gcataccaca tgcctctaac aacaactctt cgatccttga aagaaataaa tggtacactt
     3061 ggcattcgaa tcacatcttt ttccaactta tccgcattag gatcggtcgt tgtaaaaata
     3121 tagacttcat gcccatgtct ttctaattct tgttttaatg ttttgatcga tgtagcaact
     3181 ccgctgactt gcggaaagta ggtatcggta aaaaagccga ttttcatgtt tttcgcctcc
     3241 aaactagtcc ttgacctgat ttactttttt taatttgcta gagacttttt cattcagtac
     3301 actttcgtat acatttttaa gttcttcacc gattttattg atacttcgct cttttgctgt
     3361 ttgataaccg gcttgtgtcg tactcggtag ttcttgattg gtaatctgtt ggatcagtcg
     3421 gataaattct tcgtttgtct tccccatata gcagttcttg ctatcttcta accagccatt
     3481 atacactgga atatctcgaa caagtacgtt ttgtttgctt gctaaagctt ctaaaacaac
     3541 gatcccttct gtctcttcat aagaagggaa aaagaataga tctgcacctg aatacgcacc
     3601 ttctatgatc tctccacgaa tgtaaccagg aaacaggaca ttttcaggat gatcttcttt
     3661 tacaattttc cggatgttcc gaggaatcga gtacatggga acatgaccaa accaaataaa
     3721 tgtgtattcg ggcatcttct tagcaatttc tacgaaatca atgattcctt tacgttcgaa
     3781 aaacaaaccg acacaaatga ttactttttg atccggagac agtttgaaat attctctgaa
     3841 cttttgttct ttcattggat cagggtgata tttctctaag tcgatcccat tggaaatagc
     3901 ttgaatcggc acacggatac catagctttc caacaactgt tttgaataag gtgttggcgt
     3961 gatcagatag tccgcttttt ggtacaatcc taccagatat ttttttacga taggagaaag
     4021 ctggttcgat ccgataaagg aatttcggaa atcttcctct gtagaatgcg catgatagac
     4081 aacttttttt ccttctcttc tggctttatt gaccatatta tgactattga ttccgtatgt
     4141 attgatgtgc aggatgtcat aatcttcttt ttcatctagt gtataagaga taccgacttc
     4201 cgtcagagca cgtttttgat gatcaagcgc acgcccgatt ccagattttg caagcatctt
     4261 ttcactttca aaatataata ataccttcac cttacgtccc ccttcactta tatagtataa
     4321 catagcctac aaactgtcac tcaaacttaa gccctatttt tgtctattct tactgacaaa
     4381 aacgttttct taaaaaagaa caaaaaaact ggcgtagctc ttttacaagc cacaccagct
     4441 tttgatacgt tgtttatttt acatattcat gaacaagttc aacgacttct tccatcatat
     4501 cgcattcttt caatgcacga tcagcaagtt cagccatttt cgctgtgtcc aagcgtttca
     4561 ttaaactacg cgttttaaga atagatgtcg cactcattga gaactcatct agacccatac
     4621 ctactagcaa tggaacagct gtttgatctc cagccatttc tccacacata cctgcccatt
     4681 ttccttcagc gtgtgctgca tcaattacat ttttgatcaa acgtaaaatt gatgggttat
     4741 atggttggta caagtaagaa acgcgttcgt tcatacggtc tgctgccatt gtgtattgaa
     4801 tcaaatcgtt cgtacctaca gagaagaaat ctacttcttt tgcaaacttg tctgctaaga
     4861 ctgctgctgc tggaatctcg atcatgatgc cgacttggat cgtatcagat acttccttgc
     4921 cttcacttat caatttttgt ttttcttctt caaagatcgc ttttgctgcg cggaattctt
     4981 tgagtgtagc taccataggg aacatgatac gtaaattacc atgaacagat gcacgaagca
     5041 atgcacgcat ttgtgtacgg aacatttcgt cgccttgttc agataaactg atacgcaatg
     5101 cacgatatcc taagaatgga ttcatttcat gaggtaattg cagatatggt agttctttat
     5161 ctccaccgat gtccattgta cgaacaacta caggttttcc ttccatacct tctaatacag
     5221 ctttgtaagc ttcatattgg tcatcttctg ttgggaagtc tggagaatcc atatacagga
     5281 attcagtacg gtacagacca actgcttctc caccattatt atggacacca actagatctt
     5341 ttggtgttcc gatgtttgca gctaattcaa aatgcttgcc gtcagctgtg acagtttctg
     5401 cattttttaa tttttcccat tctgcttttt gtgctgcgta atctgcacct gctttttcaa
     5461 attcagcttt ttgttcatct gtaggatcga tgattacatc gccttcgatc ccgttgacag
     5521 ccaaaatatc gccttctttg actttagcag taatttcttt tgtcccaacg attgctggaa
     5581 tttctaaaga acgtgccatg atagcagaat gtgaagtacg tccaccaatg tctgttacaa
     5641 acgcttttac aaaattgcga tctaattgtg ctgtatcact tggagtcaag tcatgtgcaa
     5701 caacgaccac ttcttcgttg atcattgatg gatttggtaa agtaacgcct aataagtgcg
     5761 ctaaaatacg tttagcaacg tcacgaatat ctgcagcacg ctcttgcata taagcattat
     5821 cttccattgc ttcgaacata ccgatataca tatccgttac ttcttttagc gctgattcag
     5881 catttacgct gttgtcttta atattttgct tgatttgacc taccatttct ggatctgaaa
     5941 gaaccatcaa atgggcatca aatacttgcg cttctgcttc acctaagctt tgcgctgctt
     6001 tttcacgaat ttgctgcaat tcttcagtag atttagctaa tgcagcatcc aaacgagctt
     6061 cttcagccgc agtatcttct actgttgcct tactgaatgt taaatccggt tgaactagca
     6121 ggtaagcttt tgcaacggct acaccgtcac tagcggcaat cccttttagc atttcaacca
     6181 ttattcagat aagccttctt tcttcattgt ttcaacgatt gctgccattg catcagcttc
     6241 gtcagcacct tctgcagtaa tagtaacatc agaaccttga cctacaccta aagacataac
     6301 gcccatgatt gatttcaagt ttactgattt acctttatac tctaagttta catcagagtt
     6361 aaatttgctt gctgtttgta ctaatagtgt agctggacga gcgtggatcc cagtttctgc
     6421 tactacgtga aattctttct tttccatatt aatcggtctc ctttaagtga gtgttgtttt
     6481 tatgtgaggg ttttcttttt tagtaattca gagaatccta acaaatgcca ccctttcatc
     6541 tgtaaagatt aacatttttt ttcgttagat acaacagttt tcattcaatt tcagataaaa
     6601 gtattcaaat tattccggat tctttttcat ccttttcttc acattttata acaaacgtgt
     6661 ttatttcctt tttcttgtcg ctttctcttc tcctgacgct tatcattctt tcctttgacc
     6721 ctctttgttt tcatatgaaa cgaattactt gcttcccttt ttatttttag tataataaga
     6781 gtcaaaggtc aggaatggtc aatagagatt tcaccttttc tacctcataa atctcttaaa
     6841 aaactagggg ttattctttt acctcatgaa aaacgagatg taaaatatgg atgtacccat
     6901 ttttgtgaaa ggacgtgaat gattcatgct ttgtcaaaac tgcggcaaga atgaggcaac
     6961 gattcacttg tatgccactg tcaatggtca gcggacacaa ctcgattact gtcagagctg
     7021 ttatcaaaaa atcaaaaatc aacaaaatgg aggtctgact aatatggccc aaaatgatcc
     7081 atttggattt ggcagtttgg acgatctttt ccgttccatg tctcgccaaa tgcaacagca
     7141 aggaaattat gagcaaacac caccaacaca atttggcgga aacaacaact tcaatggtgg
     7201 tcagccacct caaggcaata cagaaggttt attgggagaa tacggaatca atatcacaga
     7261 acaagcacga caaggtgata tcgatcctgt gatcggacgc gatgacgaaa tcaaacgagt
     7321 cattgaaatt ttaaatcgcc gaaccaagaa caatcctgtc ttgattggag aaccaggtgt
     7381 tggtaaaaca gcagttgttg aaggtttagc acaaaaaatc gtagatggtg atgtaccgca
     7441 aaaattaatg gacaaagaag tcattcgttt agatgtcgta tctttagtcc aaggtactgg
     7501 tatacgagga caatttgaag aacgtatgca aaaattgatt gaagagatcc gtcaagcaga
     7561 aaatgtgatc ctattcatag atgaagttca cgaaatcgtc ggtgctggtt ctgctggtga
     7621 tggaaatatg gatgctggta atattttaaa accagcttta gctcgaggag aattgcaaat
     7681 ggttggtgct actaccttaa atgagtaccg catcatcgaa aaagacgctg ctttagaacg
     7741 tcgtatgcaa cctgtacggg tagatgaacc aactgtcgaa gaaacgatca gtattttgaa
     7801 aggtttgcaa aaacgttatg aagactatca tcatgtaaaa tatactgatg aggccatcga
     7861 agcagccgca actttatcca atcgttacgt ccaagatcgc ttcttgccgg ataaagccat
     7921 cgatttgcta gatgaaacag gttctaagaa aaacttgacg atccaaatcg ttgatcctaa
     7981 aacaatcgag aaaaaactgc aagaagcaga agaacaaaaa gttttagctt ctcgagaaga
     8041 agatttcgaa aaagcagctt attatcgcga tcagatcaat aaacttcaaa aaatgaaaga
     8101 acgtcagctc actgaagagg aaacacctgt gattaccgaa aaagatatgg aaaaaatcgt
     8161 tgaacaacgg acaggtattc cagtgggtga attaaaagaa aaagaacaga cacaactgaa
     8221 aaacttagca gatgacttga aagctcatgt catcggacaa gataacgctg tagatcgagt
     8281 agcgaaagcg attcgccgaa accgtgttgg attaaacaaa cagaatcgtc caatcggctc
     8341 cttcttattt gttggaccaa ctggtgtagg taaaacagag cttgcgaaac aacttgccta
     8401 cgaactattc ggttcacaag attcaatgat tcgtttcgat atgtctgaat atatggaaaa
     8461 acatagtgtc tctaaattga ttggttctcc tccaggttat gtcggatacg aagaagctgg
     8521 tcaattaaca gaaaaagtac ggagaaatcc ttacagtctt gtcttgttag atgaagtaga
     8581 aaaagctcat ccagatgttt tgcatatgtt cttgcaaatc ttagatgatg gtcgattgac
     8641 agatgcacaa ggaagaacgg tcagctttaa agatacgatc atcattatga caagtaatgc
     8701 cggaactggt aaagtagaag caaacgttgg ttttggtgcg gcacgcgaag gtgttactcg
     8761 ttctgtattg aaccaattaa acaactactt tacaccagaa tttctgaacc gttttgacgg
     8821 gatcatcgaa ttcagtgcat tatcaaaaga aaacttgatg acaatcgtca cactaatgct
     8881 agacgatgtc aaccaaatgc tggcagcaca gcaacttcac atcgaagttc ctacgaatgt
     8941 caaagaaaaa ttagttgact taggctatga tccatctatg ggtgctcgtc ctcttcgacg
     9001 cacgatccaa gaacaaatcg aagatggtat cgctgaattt tatcttgatc acccttctat
     9061 ccatgagttg aaagcaaaac tggataaaga tggaaaaatc atcgttactt ctaaaccaga
     9121 acgtttggct aaagaatcag ccgaagaaac ggctgaataa atccagtttt atagcgtgag
     9181 acggtatttc tttgagaaat gccgtctcat tcttttttca gaaaagtcaa aatactgttc
     9241 attttataaa aaagaaatta taataagaga aacaagcaat taaaaataca taaaagaggt
     9301 gagcgccatg aaattggtaa atgtaacaaa cagccattcc cgtcttgtat taaaccagct
     9361 tgaaaacact gatgcccatc tagtcaaggt ctacacagcc ggaaatacca ctattgtcta
     9421 cactgaagcc cctgaacata atgaaattct gctgatcaat gacaaacgta aaatccagcc
     9481 gaaagaaatc gaagatgcta aaaattattt cttagataaa atcaaagacg ccgtttacca
     9541 cgaggatgaa atcaaagtaa tcgaattggc aggccttgtt gaaatctcga ttccaaaaca
     9601 tgaattaggc gctacattag ccgtgtaaac aaattttgaa cataataatt aaagagcgtg
     9661 ggacaaaagt aaagaacact tttgtctcac gctctaaaca cgtataaacg gcgaaagcag
     9721 aagcaactcc ttcggaaata agctgaaatt cacgaaaatt gcttttcaaa tttttgtgaa
     9781 ttccagctta tttcttggag ttaaacgctt ctgtctcagc ctctttttct ttctagcttt
     9841 ttgatttttc tcttttcttt tgtacttttc tcttttcatt ttcagttcta cgcgtgtctt
     9901 tatttttaac tttttcctct ttatggatga ttgatcgtac tttttgtgca atttgctcgt
     9961 ttttgtccca gtcttttaca tctaataatc gacataatcg taacatctct tcttttttct
    10021 gtttttttct ttcacgtctt tgccattctg tttccatgtg tcacactctc ctttctttaa
    10081 tcatatgtac gcaaaataat ttttatattt ttttgattag acatttctat gattttaata
    10141 tagtaaaaag agccgtcacg gaaaatccgt cacggctcta aacaaaatat gagaggctgt
    10201 ttttgtttgg ctattcttat tacttgatgc agggcagctt gtgtcaagac ttctttacat
    10261 caaacttttt aattttacat tgggatattt atccgcaaac cagcgttctg caaattggtt
    10321 ctcgaataag aacaacggtt ggtcgaaacg gtcacgagcc aagatattgc gacttgaact
    10381 catcttctca tccaaatctt ctggatcgat ccagcgagcg attttgtgtc ccattggaga
    10441 catgattact tcggcgttat actcatttaa catacgatat tggaatactt cgaattgtag
    10501 ctgaccaaca gctccaatga tatattcttc cgttagatat gttttataaa gctgaatcgc
    10561 tccttcttga actaattgat agattccttt gtggaaggat ttttgtttca tgacattttt
    10621 tgcagtcact ttcataaaca gctctggagt aaatgaaggg agttcttcat aagcaacttt
    10681 tagttttcct tcgaacaatg tatccccaat ctgataattg cctgtatcat atacaccgat
    10741 gatatcgcct gctactgctt tttcgacgtt ttctctcgaa tctgccataa attgtgtgac
    10801 attgcttaat ttcagttttt tatttgtacg ttccaaccag acatccattc ctcgttcgaa
    10861 tgttccagaa cagattcgga caaaagcgat tcggtcacga tgggctggat tcatatttgc
    10921 ttggatcttg aatacgaaac ctgaaaattc ttcctcataa ggacttacca tctgatcatc
    10981 ttgtgttttg tgtccataag gagctggtgc caattcaacg aaagtctcta aaaaagtctg
    11041 tacaccgaag ttagtcaatg cagaaccaaa aaatactggt gtctgatcgc cacgagcaat
    11101 tttttcttga ttaaatgcat ctccagcctc tttgattagt tctacttctt ctaagacttg
    11161 ttcatataag gaattttcag ttagaggatg attttcggga agcagtccat cttcatccaa
    11221 ttctactaat cgttcatcct gataattttc cggacgataa aactctacac gattatggta
    11281 gatatcataa agaccttcta gccctttacc cataccgatt ggccaattca tcggataaga
    11341 ttcgatatct aatagctctt ctaattcttc taacaattcc aatggttcac gaccatcacg
    11401 atctagtttg ttgataaaag taaatatcgg gatgccacgt ttttttacaa cttggaataa
    11461 tttctttgtc tgtgcttcga tcccttttgc actatcgatt accatgaccg cactatccac
    11521 ggccatcaaa gtacgataag tatcttcaga aaaatcttca tgtcctggtg tatccaagat
    11581 atttactcgt ttcccttgat aatcaaactg cataacagaa ctagtaacag aaatcccacg
    11641 ttgtttctcg atttccatcc agtctgattt ggcaaaatta cctgtttttt tgccttttac
    11701 ggtcccggct tgacggatag ccccaccaaa caatagtaat tgttctgtaa tcgttgtttt
    11761 cccagcatcc ggatgggaaa tgatcgcaaa agttctgcga ttgtccactt gttgctttaa
    11821 ttttggatta ttcataacct aactccattt cttccattca attatttata ttttctctag
    11881 gcacagattg tccgatctct taaagaaaga caatttcata tagggctgtg acaaaagccg
    11941 tgtagcagtc tctttttccg agtaaacggc gttctagaga cagcctatca gcaataagcc
    12001 cacccaagcc aaaaatacga aaagctgttt ttgcttggct gctcttatta cttaatgcag
    12061 gatagctttt tcacaacctt tgtcaactac tacaactgaa tcagtatagc atggattcag
    12121 cgaatgaaaa agccaccaaa ccataagagt caaatatttt tttgtcagct gactaaaaga
    12181 aaagacgtgt tgcttgttta ttcaagcaac acgtctttca taaccaagtt tccaattatc
    12241 ttcttggctc gtcatcttcg aattcttttc tgaagtatct tcgttcttct tcaggttccg
    12301 cttctgtttc ttccggatca tgaaaaatga cacggatttt aaggattcga gaaccttcca
    12361 tttcttcaga aaccagcgta atattatcga catcaaacga taacttttcg ccttcatccg
    12421 gaatcatgcc taatgccgta atcaaataac cagccatcgt atctacatca ctcatatgca
    12481 gatcactgtc aaaagcttca ttgaactcat caatcagcat acgtccttgg atgatatatt
    12541 cgtgttcatc gattttttca taaagattct caacttcatc tgattcatca tcgatttctc
    12601 cgacgatttc ttctaaaagg tcttctagtg ttgctaaccc aacgacgccg ccatattcat
    12661 ctaataaaat agccatctga ttctgcgtct ttttcatttc atataacaga tcatcaataa
    12721 agaccgtttc aggaacgaat aaaggctcct gcatgatttt cttgatatct aaattatcaa
    12781 agccgaattt atgggcagct tttagtaaat tctttgtatg aaggattccg actactttat
    12841 ctttgtcttc gttataaaca gggattcttg aatagttttc ggataaaact tcattcacat
    12901 tttcttccac agtgtcgttg atatcaatca taaatgcatc cgttcgtggg accatgactt
    12961 ctcgggcaac tttcgtatca agagaaaaaa ccccttggag catttctaat tcttcattat
    13021 tcaatacgcc ttcgttttca agcatataac gcatttcatc tctggtcatt tttgaatctt
    13081 catcatcaaa agtcatcggc gtgatacgcg ccaataaact tgtggaagca gaaagcagcc
    13141 agacgaaggg cttagctact acaccaatca agcgtatcag ccctgaagtg aactttgcga
    13201 cttcttctga tttatttaaa gcaatacgtt tagggtacaa ttctccaaaa acaatcgata
    13261 cataggtcaa aatagccagc acaatgatat ttgcaatatt ttttgccgct gtgccgccac
    13321 ctaataccgg agctaaccgt tccgataaag tattagctag tgaagcacca gacaaaatgt
    13381 tgaccaaagt gattcctact tggattgtcg ataggaaatt atttggattc tgcaatattt
    13441 ttaaaagctt ttgagatttc acatctcctt catcagcttt ttgctcgatt cgattcttat
    13501 tgatcgaaac aacagcaatt tcggaagctg ctaaaaaagc attgatcaat gtcaaaacga
    13561 tcaataataa aatctgcgct aacagcgact gactctcagg gtcagcattc ataatcgcat
    13621 tctctcctta taaaattaat ttttagttta cgatagaaaa aaacgtggca ctatagccac
    13681 gttaaaatgg tatcacaaag caattttctt tgcaataaaa acaagtgtac taaaactacc
    13741 tacgaggtcg tagcaatttt cttcttgttt tcattttgct tgtcttcttt gacgattttg
    13801 attatatagg aaatgatcgt cttgcagatt gcgtaaaaag gaacacctaa gaagatacca
    13861 agcaatccgg caatatttcc agctactaga agaacgataa tgatcgttaa tggatggatt
    13921 ttcagtgatt tcccaattac attaggataa atgatattcc catccaactg ttggacgatt
    13981 agcacaacca cacaacaaag taatgctttt accggatcat tgaagactgt tactagaaat
    14041 gcaggtgcta gtcctagata aggtcctaaa tatggaatta ggtttgtcaa tcccgcaatg
    14101 accccaaata agaaagcata atccacacca attaccatat atcctaaaaa tgtgaaggta
    14161 ccaacgaata aacattcgat tgcttgtccg ctaatgtaat tagccaatgt tttattcagt
    14221 tgccccaata actccacaat ttggtttctt cttttttctg ggaaaaactg ttggatgttt
    14281 ggcactagtt tttctccgtc tttcaacata tagaacaaga tgaatggtgc tgtaatgatt
    14341 actacggttg ccgaagcgat cgttccaacg atcgaaccta aactattgga tactccacta
    14401 aggaattgct gaatgattgt cccgtaagaa atgtctaatt gctcaaaata tttattgata
    14461 tctacttcct tgaacaaagg caattcggct acctctttta accagctttc tacctgtttg
    14521 atgaaatcag gcatattaga tgccaatgag gaaatttgct gtactagact tgggatgaca
    14581 ctaagtaaaa tccaaataat tgctgcaact aaaaggagca gcacaattgc gacagcccat
    14641 attcttttga tttttgcttt catcaacagc ttgacaatag gattcaataa atagtaaaga
    14701 aagcctgcaa tcagtacagg tgcaaataac gtggaaaaaa atgtcccgat cggttcgaaa
    14761 ataaagttta tttctgatga aatcatgatc aatgcagcta tgatcaataa ttcaagcgac
    14821 caaaacatta gtttagactg tctaagtttt tcaaacaata gatttcctcc tttctttctt
    14881 actagtatat attatattcg ttaaaaattc ttttattctt tttttatttt tgaaaacttg
    14941 ccatactttt tatttgtttt attttccagt atactatacg aaaggaaggt ggctagacca
    15001 tgaaagttgt acaaacaaaa gatacaatga gcaatattta cttagatgct gtacggatca
    15061 ggcatcaagt ttttgtagtc gaacagggtg tgcctttgtc tcgagaaata gacaaagatg
    15121 aagcccattg tatccacttc gtcctgtact cagataaaaa agaaccgcaa ggaactgtac
    15181 gtcttttgcc tttagaaaac ggaaaaatga aattacagcg gatggcgatt ctttctgaat
    15241 acagacacca agggttagga aaaatattga ttgaagaagc agaaaacttt gctaagaatc
    15301 aaggctataa tacgatttta ttaggggcac agtccacagc tgaaactttt tacgaaaaac
    15361 taggatatac agcttacggc gatccttttg aagatgctgg catgcctcat atttatatga
    15421 aaaagacttt ataaaaattt atttcgaaaa aagcaaaaaa cagcaagctt ttgagaaaaa
    15481 tctctcactg gcttactgtt cttttttgtg tttacttacg cgacaattgc ttgtaacggt
    15541 tataccaaat atcaatatag ccttccgaga acgggccatc tccattattg atccagtcaa
    15601 ccagaatctt cacattttct tttaggatga agtcaatatc gttcgggtaa ttcatcattt
    15661 tgctgtgcat ctcatattca tcgacatcca atagtcgttt ctcgccgtca ggaaatacct
    15721 tgatatccaa gtcatagtcg atatatttca aggcctcatc gtccagcaaa aaaggtgaag
    15781 ctagattaca ataataggaa accccctttt ctctgatcat tgctattaca ttgaaccagt
    15841 attttttatg aaaataaaca atcgctggtt cgcgcgttac ccatcggcgt ccatcagatt
    15901 cagtgactaa tgtgtgatca ttcacgccga tcatagagtg ttcgcttgtt tttaatacca
    15961 tggtatctcg ccacgttcga tgcaatttgc cgtcgtgctt gtaactttgg atcgtcacaa
    16021 actctccttc ttttggaact cccatagcaa cccttcctta agcagcattt tgtgctttgt
    16081 gtttgggtgt tcattacacc gacatcataa ttattatatc aataaaaatg agctttgcct
    16141 agaaaggatt cctattgttt cgatttttct ctaactgatc taccagtttc atttggacct
    16201 taggaaaaac gaccgaatca aatgcatcag gcttcaacca cttcgaagtt ttgttctcgg
    16261 taaattcttg ttctgctccc tcggttgccc gtccgtaaaa aaggagaaca tgccatttca
    16321 gatgactaaa aatatgagta acttctccta aatgtcgtgt ctgccaaaca aagagttcct
    16381 gcttttcaaa tgggagattt tgctcttctt ctgctactag gtcatcaaac agatcgattt
    16441 ctggctttgt ttcccattct tttttcaatc gctcatactc ctcttgtgtc acttccatca
    16501 ttggaaatgt ccacatattc gccagcaatt tttggctatc tctttcttca aaataatacg
    16561 ctccagaatg attttgaaga gctgcactga tataataaac gtcttttggc tttgcttttt
    16621 ttgtttttac aggaaaactc gtttggattc ctcgtttgtt agctaaacaa aaggcttgga
    16681 tcggacaagt ctcacacttt ggagaagttg gtgtacagat agctgaacct agatccatca
    16741 ttgcttggtt gaattcacct ggatgctttt catcaatgat tttccgcatc gcttcatcaa
    16801 agattttcct gctagaagct tttgcgatgt ctgcttcaat gcaaaacaat ctgctgacta
    16861 ctcgcatcac gttgccgtca actgcaggtt ctggaagtcc aaatgcgata cttgcaatcg
    16921 cgcctgtcgt atatggtcct attcccttca atgagctaat ttcttcaggc gtttgcggca
    16981 tttccccatc aaattctgac atgatctgtt tagcagcagc ttggatgttc cgggctcttg
    17041 agtaataacc aaggccctcc catgctttta aaagcttttc ttctggagca tttgctagct
    17101 cttcaattgt aggaaaccat tccatgaaac gataaaaata atcgatcact gtgtctactc
    17161 tggtttgctg aagcatgatc tcagagatcc agatacgata aggatcacga ttatagcgcc
    17221 aaggcaggtt tcttttttct tgttcatacc attgaatgaa ctgatcttga aattcttttg
    17281 tttctttatc tgtccatttt tcccattctt tattcacttt cctcttcctt ttccgtaatg
    17341 aatcgctgga tcatatccaa atcaaatcct ttttgatata agctttgctt tacttttaga
    17401 tttctttttt taggttcaaa ccttaaattt cttcgccata gtttatctcc ttgtttgact
    17461 aaataatcat attctatttc ttgatctgct tcttgcggaa gatcagctat cgcttgttgg
    17521 atcacttctt gagaaaagcc tttcgtcatc aatgtttgtt ggatttttct caataattct
    17581 ttttggcttt ttccgtgatt ttttccaaac gctttttcgg ctgtctgatg tgcattttga
    17641 acttggtctt caaatgtata ttggctgagc gcttgttcaa tgatttcagg tttcaaacct
    17701 ttttgctgca tctgctgcgc taatttcttt ggtcctttgt cacttaagcg catattcgtg
    17761 cgcacataac tttccccata gaccaaatca tcgacaaggt ccaattcttt taagcgggct
    17821 acgattttat ttcgatcttc ttgaaggatt tccttttctt ttaagtatgt tcggatttct
    17881 ttttccgatc gcaactgata acttaagtaa ttcattgcta ggtgaaaacc gaaatcagct
    17941 ccgctactct tcttgatctc ttggatagtt tcttcgtcta attcccgtcc ttttaataat
    18001 cgatagcgta ctaatacatc ttcagatact tgtaaagctt ctccttcaga taaatctacg
    18061 cgatagaaag ggcctttgcc tttactgatt ctcgtaacgg ttaacataaa catcctcctc
    18121 gaacatatat tctctatcat accacagaaa atccccgtaa tcaggtatta tatgttagaa
    18181 taaaccacag aaaaaagaaa aagggagata cttatggcag aaacaattgt caaaactgga
    18241 caaacggttc ctttgaaaat caagcgttta ggcataaatg gtgaaggaat cggttatttc
    18301 aaacggttga tcatatttgt tccatatgca ctaccaaaag aagaagtact agtgaaaatc
    18361 acgaaagcaa ctcctcgcta tgcagaagga gaattaataa aagtcaaaaa aacaagcaaa
    18421 gatcgcgtag tggcaccatg tcctgtttac tatgaatgcg gtggctgtca attacaacac
    18481 ttagcttatc aagctcaatt ggattttaaa aaggatctgc ttttgcaagc attggaaaaa
    18541 ttcaaaccag caggctttcg atcttatcaa cttttgccga cgatcgggat ggatgagcct
    18601 tggcattata gaaacaaagc acaatttcag ttacgaaaaa ataagcagac tcaaaaaatt
    18661 gaagctggtt tatacgaagc aaactctcat cagttagtcc ctataacaga ctgcttggtt
    18721 caagaacctc gaacacaaaa agtaatgaat actgttgttc aactattgaa taaatttgat
    18781 gcacccatct atgatgaacg gacaaacagt ggaatcttcc gcacaatcat ggttagaatc
    18841 ggtgttaaaa ctggggagtt acaagttgtt tttattactc gcagtaaaaa attcccgcaa
    18901 aaaaacgcaa tgatccgtga gatcaatgaa cgactgcccg aagttgtttc aatcatgcaa
    18961 aatgtccagg cacaaaaaac gtctctcgtg atgggggatg aaacgatcca cttatgggga
    19021 aaagaagcca ttgaagaaca aatcaatgaa gtgactttcg atctttctcc tcgtgccttc
    19081 tttcaactca atcctcagca gaccgaattg ctttatggcg aaggaatcaa agcattagat
    19141 gtccagccta atgaaacgat cgtggatgct tattgcggag ttgggacgat cggcttaagt
    19201 gtagcaaaac aagccaaaga agtacgggga atggataccg ttcctcaagc aatcgctgat
    19261 gcaaaaaaaa atgcggaaag attaggatat cacaacactc gatatgaagt cgggacagca
    19321 gagcaagttc tgcctaaatg gttgaatgaa ggttttcagc ctgatggtat catcgtagat
    19381 ccaccacgta cgggtttgga taacgcattg atccaatcat tattgaaata ccctccaaaa
    19441 aaacttgttt atatctcctg taatgtttct acacttgcca gagatctggt taatttaagt
    19501 aaaaagttca agattgaata tttacagtcc gtcgacatgt ttcctcagac tgcacgttgt
    19561 gaagttgtcg tgaagatgac gagactcgct gactaaatga aagaggttgt gaaaaagtct
    19621 gccctgtatc aagtaataag aacagccaaa caaaaatagc tcttcatgtt ttatgaatac
    19681 tgtacttgtg accgaagagt taacaggagg acactattta tttaaaaaaa gggttgtgac
    19741 atagtttttg tcacagccct ttttgattat tattggatat tcacgtctat cacttgataa
    19801 aatgcatttg tcgtatcgct gatattccat actgctaaaa tcacattata tccttttcga
    19861 tcctgcggaa gtgtgatcgt ttggttgact gtttttggtg gaacagttgc tttgtcgtct
    19921 atttttgtga tcaattcaaa atcactaaat ttcaaaggtt gattaggatt ccaagttggt
    19981 ttggtaatgt agtaatccca agtacttgtc cgatgttgtg ctgttaaatt ccaagtaata
    20041 tttaatgttc ctggattaac catgttttta tgccatctgt tagatgtctg ttcatctaaa
    20101 ggctcaaagc cgctgactcc tgcactagca atttttccat caatgaatgt atttttggta
    20161 gcttcgatac tttgcggttc ccattgtgct cttccgacat tttgatttaa gtttcctgct
    20221 gctgatgttc ctaggtatgc acggcttcct ggagaagtaa tataaccatg cgcaaaacct
    20281 ggaatgctaa aaccgaacaa ccctaaacta actaacccaa taaacaataa cgtactaatc
    20341 tttttcatgt agaaacctcc ttcatttgat aaccacattg taaacgcttt attgtattaa
    20401 cacaaacatt atgcttttat aattttatgt tgaagtaaaa aaggtgaaaa aagtaagatg
    20461 cagattataa cagaagtaga cgagaaaagt acaaatagaa aacatgagaa tcaaatcata
    20521 tgatcttttt caaaaacatc attcatataa acaaatgttc aactttttaa taacaacaat
    20581 tatcaatttt tttcttactt cacaaactaa atacaggtct tgctgtgagc cttcatggta
    20641 aactaatatt atagaaagga gttagtaaaa tgaagatgat aaaagatatt cgtttttggg
    20701 tttgtgttat aggaattgtc atattaggct ttttatcagg gttgcttagc ggaaatcccg
    20761 gaaaatatta ctattctttg caacttccac catttgcacc acctagctgg atatttgggc
    20821 cgatgtggac cttactgtat atattgatgg gtatttctct ttatttgtta cttaatcaca
    20881 acaacaaaaa acagcgaaac aatcttgtcg gtctttttgt gattcagttc atttttaatt
    20941 ttatatggtc tgctctattt tttaatttaa gaaatatttt cattgcagca atcgatatta
    21001 cacttttagt tattttcttg tcagtgctta tgtatcagtt gtggctgcat catcgcctgg
    21061 caatgtggct catgattcct tattatttat gggtactatt cgcaactcta ttgaattatt
    21121 caatttattt tttaaactaa gcgctaaaaa gagacgtcct gtaatttaac acatagaaaa
    21181 agtgccacct aagttagatt ttttgtctaa tttaggtggc actctttatt aaccaaacta
    21241 tttttttcta gtacccactt gttgttccat actcattatt ctggtcataa ccataaccat
    21301 tttgttcctg tccagcgtaa ggatcataac tttgagtatc ttgacttggt gtatagtact
    21361 gacttgtatc ttctgtttgc tgataatagg tattcggatc ttggtactga tcatatgtgt
    21421 tttctgaata ggaagaataa tcggtgtacc cttcactatt cgatccatca ccgtttgtcg
    21481 aatcgccatc ttccgaatca tcaaagaaca tcattgaagc attctcattt ttcaaattag
    21541 gtaatgtatc gcttgttttt atatttaatt gtttcttcag ttcattttgg atcgaaagaa
    21601 gatcattttt tcctaaaatt tgatagtaga tatcattgat catttgtcct tcgccttgca
    21661 actggtcttg tttgattgtt tcaaacgccg gggtgtagtt cgtcgctaca tcaagcatgt
    21721 catcccaatc taagtccgtc gtcacattgt tctcaactgc ttttaaaatt tttctataat
    21781 tgcttacgcc atctaaactt aaaactttat tgacgatttt cgtcactact tcccgctgac
    21841 gacgttgtcg accgatatcg ccttctggat cttcgtgtcg cattcgagaa taggctaaac
    21901 ctttttctcc attcaatgtt tgttcaccta ctggtacatg gacaccatcc aaagtaaagt
    21961 caattttatt attcactttg atgccaccga ccgcattgat caaatcttcc atgccatcca
    22021 aattgatcgt tacataatga tcaataggaa tatcgagcag ttgctcaatt gaatccatgg
    22081 ccatttcgac accaccaaat gcgtaggcat gatttaattt atctaccgtt ccataaccta
    22141 cgatattcgt ataaatatct cggtccaaac tgatgatcgt tgattttttt tgtttcggat
    22201 taactgttac taccatcatc gtatcagaac gtccttgttc cgttcttcct aatccaccgg
    22261 tatctaaacc taatagcaaa accgaaaaag gttctttgtc atcaatgctt actctctcag
    22321 aacgtttgga ggatattcga ttagagtttt tagaaatttt gtttactgtt tggctcgctt
    22381 ctcccataaa tctgattccg tatgctgata atccggcaat cagcaaaacg aataccccta
    22441 acaaggttaa tagaactttt tgccatcgct tcataataca cctctgtttc acattttcta
    22501 aaatattgtc tcatataact gttcaatcag caaattcact ttttaatcat aagaaaactt
    22561 taaaaaacat ttttgtgtaa cgattattat tttttaaaat ttctcaaaat tcttaactat
    22621 cataccatac cgtgaaaaag atcgaaagac cgaatacacg cagttcgcaa tcgttttatc
    22681 tttttttatg tattttttcc agaaaaagcg aacaacaagg tattttccaa ataatcgtgc
    22741 agtaaaatga tcgcacatac ctttcccatc tttttaacag tctttgccta ccgttcagat
    22801 tatcattcga tttcacgaca atttcttcct atgactggtc cttttgttaa acccatcttc
    22861 tattcgtttt atttccagct cactgcatgg ctcttaaaat gactcgaact agtgattgca
    22921 ttaccatcat cttttaatgg atagatatgc tcctcttcaa agtaaaacga ttttctttta
    22981 aagtaataga attgacatgg taatgagtat gtatactagt ggtttcacaa atggtctttt
    23041 attcaacatc caaaaactga aatttcttta aaatccatta ctaacgaaga aaatgttcct
    23101 tgcttgataa ttagttagga catgtcaagt gaacaaatgc atttacttag ccaaaaaatc
    23161 taaaaacttc ataaatagac aaaaagacac gactctctaa ga
//
LOCUS       DACCXO010000036        22992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.037, whole genome shotgun sequence.
ACCESSION   DACCXO010000036 DACCXO010000000
VERSION     DACCXO010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.037"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(109..564)
                     /locus_tag="IVY70_001302"
     CDS             complement(109..564)
                     /locus_tag="IVY70_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958439.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="IVY70_001303"
     CDS             complement(718..1926)
                     /locus_tag="IVY70_001303"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP7958440.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="IVY70_001304"
     CDS             complement(2038..2370)
                     /locus_tag="IVY70_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP7958441.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="IVY70_001305"
     CDS             2518..3393
                     /locus_tag="IVY70_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7958442.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="IVY70_001306"
     CDS             complement(3475..4056)
                     /locus_tag="IVY70_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP7958443.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="IVY70_001307"
     CDS             complement(4062..5321)
                     /locus_tag="IVY70_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP7958444.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="IVY70_001308"
     CDS             complement(5323..6369)
                     /locus_tag="IVY70_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958445.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6604..6879)
                     /locus_tag="IVY70_001309"
     CDS             complement(6604..6879)
                     /locus_tag="IVY70_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP7958446.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7122..8432)
                     /locus_tag="IVY70_001310"
     CDS             complement(7122..8432)
                     /locus_tag="IVY70_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP7958447.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="IVY70_001311"
     CDS             complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="IVY70_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP7958448.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9978..10658)
                     /locus_tag="IVY70_001312"
     CDS             complement(9978..10658)
                     /locus_tag="IVY70_001312"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP7958449.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10707..11342)
                     /locus_tag="IVY70_001313"
     CDS             complement(10707..11342)
                     /locus_tag="IVY70_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7958450.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11408..12811)
                     /locus_tag="IVY70_001314"
     CDS             complement(11408..12811)
                     /locus_tag="IVY70_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP7958451.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12798..13829)
                     /locus_tag="IVY70_001315"
     CDS             complement(12798..13829)
                     /locus_tag="IVY70_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958452.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13860..14081
                     /locus_tag="IVY70_001316"
     CDS             13860..14081
                     /locus_tag="IVY70_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP7958453.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14246..14983)
                     /locus_tag="IVY70_001317"
     CDS             complement(14246..14983)
                     /locus_tag="IVY70_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP7958454.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15136..16476)
                     /locus_tag="IVY70_001318"
     CDS             complement(15136..16476)
                     /locus_tag="IVY70_001318"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP7958455.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16713..18236)
                     /locus_tag="IVY70_001319"
     CDS             complement(16713..18236)
                     /locus_tag="IVY70_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP7958456.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18614..19225)
                     /locus_tag="IVY70_001320"
     CDS             complement(18614..19225)
                     /locus_tag="IVY70_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7958457.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19348..19473)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19555..20271)
                     /locus_tag="IVY70_001321"
     CDS             complement(19555..20271)
                     /locus_tag="IVY70_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP7958458.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="IVY70_001322"
     CDS             complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="IVY70_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP7958459.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20830..21627)
                     /locus_tag="IVY70_001323"
     CDS             complement(20830..21627)
                     /locus_tag="IVY70_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP7958460.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21597..21968)
                     /locus_tag="IVY70_001324"
     CDS             complement(21597..21968)
                     /locus_tag="IVY70_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP7958461.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="IVY70_001325"
     CDS             complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="IVY70_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP7958462.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6446 a         4914 c         3899 g         7733 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccg ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gaaaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaaatt aaataaaata
     6481 catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt tattcctttt
     6541 ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc tttttcaact
     6601 gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta ctttgctagc
     6661 tggaatttcg atttcttcac cagtttgtgg gttacgacct ttgcgagctg cgcgttcgcg
     6721 aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta aagcatcttg
     6781 aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta aatcagtagc
     6841 tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac
     6901 aagagatatg atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa
     6961 agaaatattg attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc
     7021 aaggttttta ccaattttca catgcttttt ttagtcatag aaacgtaaca aacgaaactg
     7081 tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg
     7141 ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa
     7201 cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt
     7261 ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt
     7321 ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga
     7381 ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc
     7441 actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct
     7501 tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg
     7561 ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga
     7621 atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt
     7681 gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca
     7741 gctgtatcta tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg
     7801 tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg
     7861 atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg
     7921 tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc
     7981 cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca
     8041 tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat cactggttta
     8101 ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg
     8161 ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc
     8221 tgatccataa aaggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct
     8281 cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg
     8341 acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg
     8401 cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc
     8461 tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt
     8521 atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag
     8581 ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat cggttgcttt
     8641 ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa atccagtatt
     8701 ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag ctggtttttc
     8761 ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct ctaatacttt
     8821 cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat gtttatgtga
     8881 aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa aggcaccgaa
     8941 gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt ttgtatcgat
     9001 ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt cttgttcagg
     9061 attgatagac aacactttga catttacatc atcattgaca gccaatacat cacttggttt
     9121 tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc cgcctaggtc
     9181 gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat ctccctcatg
     9241 gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta ccgctttatg
     9301 cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc tcaatgtttt
     9361 gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg cagggacaaa
     9421 accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa caggtgcttc
     9481 gatgaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt tagcatctaa
     9541 acggcgtttt gaaagtaggt agctgccgtt ttctttatct ttgccgatcg tactgataac
     9601 gactaaatct aatacatcac cgacttttac tacttcgctg atatcttcta ccggcaaagt
     9661 tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc cgacaatcac
     9721 ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt ttacttcttg
     9781 tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat caaattctgt
     9841 cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt tttagtttaa
     9901 ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa caattgtttt
     9961 tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac agcttttatt
    10021 gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc atcctctgct
    10081 tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg tatctctttc
    10141 gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa acgtctttct
    10201 gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg taatacagct
    10261 gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt tcgttggaca
    10321 gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc ttttgtgaca
    10381 tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg ctgtccatct
    10441 tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc ttcttcatct
    10501 gaaaaagata cattgttcgt aatagctaaa taagttactg cgcgatacat ggcaccggta
    10561 tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact tttcccagat
    10621 gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct ttttctcaga
    10681 aaaaaagccg caaagcgcgg ctattattat ttaattctta attcttgacc agggtaaagc
    10741 ataaagttgt ttggatctaa gccattcaat tcaaacaatt gatcagtggt gatccctgca
    10801 cgctcagcca cttgttttgg accttcgcct gcttgaacag tcgttgttga accggctgca
    10861 gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc ttgataagtt
    10921 gcctcacttg aggaagtcgg tgttgtctct gctgcaatac tgctttctga cgttacacct
    10981 gtatcttgag acacagtaga ttcttccgcg attgtactag attctactgt tgaagtctca
    11041 gttgactcct cttttgttgt acttgtagaa ctttctacta ctgatgtcga agtttcagaa
    11101 gaagttgctg ctgttgcatt gttgcttgat ccatttctga tccagaaata agcacctgct
    11161 ggaatagcga tacataagac cagtaaaatc aaaaatagcg aaagaaataa tgtatttcct
    11221 ttcttttgtt gtctctgaga acttcttgat gaaacttcat catctgtatc gtagattggt
    11281 tgttcccatg gttcttgtgt ttcgttattt tctgtagatt gatgtctatc ttttctgctc
    11341 actgttttac ctcctaaact ctatcgtgtg tattatacca taggaaaaag tgtcttgtcc
    11401 taaaaaatca atgttccatg ttttttttaa ataaatttag taaaatcgtt tcccaatgaa
    11461 gttgttcagt cttagaaaaa agcttcctct ttggcagaag ttctgctttt gctccatcaa
    11521 tatcacaaca attctgcggc ttttcggaga gtttttcgcc aaaataagct aagatgaatt
    11581 ttctgcggca gttttcttca ttaatatagc gaagcatctg ctgcaggcgg aattcttttt
    11641 ctttttcctg acgtttgagc cgttccaccc aattttctgg tttttcagac tgctggatca
    11701 gctccatcca ttttttttgc agctcatcaa agggagcttg ctttgtggca tattccaaat
    11761 aaagctcaaa actttgtcgc tgttctctag aaagttgatt aaagaaataa tggatacgtt
    11821 catccccact ttgataaaaa agaattgctg cactttcttc ctgatctctg cctgctcgac
    11881 cgatttcttg tacatagttt tctagactgt ctggcaaatc ataatggacc acataacgga
    11941 tatcagattt atcgatcccc ataccaaatg cattcgtagc aatgagaaac tgcagctgat
    12001 tttttacaaa ctgctgttga agttgtcttc tttgagctgc atcaagacca ccgtgatagt
    12061 atccaactgt aaaacgttcc ctgaataaat gatacaatcg ttcgacttct ttttttgttg
    12121 cacaataaat gattgccgct ccttgtgctc tttccatgaa gtgttcaagt tcttgttctt
    12181 tttgctgcgt tttccgaaca aaaagcgaga tattttgccg attgactgat tgtctgatct
    12241 cttttggtgg tcgatcaaat agtacatgac gtatatctct tgctactaaa tctgttgctg
    12301 tcgccgttag tgccagtgtg accgggtttc ctaaaagctt tcggattttt cctaactgtt
    12361 gatattctgg acggaaatca actccccatt gagacacgca atgagcctca tctactacat
    12421 ataacgcaat attttgtttt ttcagatgct ctaaaagaga aggattagtc aacatttcgg
    12481 gactcaaaaa caaaaattta tattgcgata gatgacggag gacgtatctt ctttcagatt
    12541 gcgaaagtaa actattgaaa gcagctactc gtttctcccc tcgtttttgt aattgcgtca
    12601 cttgatcttc cattagagaa agcagcggtg acacgatgat gaccagacct tccattaaat
    12661 accccgtcaa ttgatagcaa aggctttttc ccgttcctgt aggcaaaata gcgagagtat
    12721 cttctccatc aagcaacgcc tgaataattt cttcttggcc gggacgaaat gaatcaaatc
    12781 caaaatattt ttttaattca tttattaaca ttctgtatcg cttcctttct caatagatag
    12841 atttgataca aacgaaattc tccataatct aattgtccat ctacattcca ttcgctaaag
    12901 cgccactctc gcacggaatc cttatttggt tccaaacgtt cgattgtttc tgcagagaga
    12961 atccactcgt atggaaaatc atcaaaaaac aaggcccact caataagatg atctgttact
    13021 gttcctcttt ttaaatgacg gattgccatg atctggtcaa ttgtctgacc attcataaac
    13081 atctgcttgg tgatcatcat gctttgattg aaattttgct gtaataatga atcgatcaaa
    13141 gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg gatacatctg
    13201 ctttgttgca gataaacctc tgactcatca ttgttttttg ccagctgata aggaagcagc
    13261 cctgttcgct catttccaga taattggtta gctaaaaaat ctgctgcttc ttctggtatc
    13321 tccgaaaaca gctgcaccaa actctcatac gcagtatcaa tcaaaatttc acgtggcaat
    13381 tggctttcac taagccattt ttttagttgg aaagtataaa aaggacttgt ctctgctgga
    13441 agatagtctt gaatacccgt tgccagatta gaaattactt gaacagcaaa tttaatgagc
    13501 cgccaactag tttcccctgt tctgccatag cggtcaaaac gcaaaccagt atgttctatt
    13561 tgatgttcgg acaacatatc agcccctgca ggcgttagct ttgcttcatt ttcatttatt
    13621 tcgatccaac cttgctgcgc aattttttgc aaaatttgat aaaacttatc ctgcttcaat
    13681 tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa atccataaat aagtactgaa
    13741 gtcgttcgtt ttcccacaag gagttggtaa agagaactaa tccgcagctt gtgtcctgat
    13801 gaaaatagcg ataaaataaa aacaatcata ttattctcct taagttagga tggttagaaa
    13861 tgtcactact atgtaaactt gtacctgaac gctgtattgc atgcggtctt tgtcaaatcg
    13921 aagcgccaga tattttcgat tatgatgaag aaggaatcgt tttatttgcc caagaaccag
    13981 aggctcatca acagttcgtg atgccggaag aagaaacata tgtaaaaaaa gcgtatcaaa
    14041 aatgtccagt tcgagcgatt ttattagaaa ataaaatata aatataaacg aaactttaaa
    14101 aacgtacact aagttctcta aacttgaaaa taagccggga tctgtttctc ttgttccgtg
    14161 aaatcccgac ttattttgaa agattcattt gctatctata gttaattgac tagttttttc
    14221 acggacccta taggacccat ttatattatt tcaacttttt cagccagttt tccaatgtat
    14281 tgacgatcac agtttgctgg tctgcgttag aaatttctgc ctctccatct cctttttgct
    14341 caccatagct gccaaaacct ccatgatttc cgccattgat tgatacatac tctgttcttt
    14401 ccggtaaata ctgcttagct tcttgataag cttcctgatt caatacttta tcttttgtcg
    14461 ccgtgatcga taaaacagga acagctgatt ctgtcaatgt tccttttttg tcgggataac
    14521 ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg attcttattg acataacgac
    14581 ttgccattac gccacctaat gaatggccac cgatcacata atcggaattt ggaaaatctt
    14641 ctttgacctt atccgctttg tttccgccca tcactgctag atcaagtggc attttgacaa
    14701 tagcgactga aaagccgttt tttgctaaat cttgcgccca aatactataa ctttctggct
    14761 ctacgagagc accaggataa aaaataagta atggagactt tccatgattc tttttctctc
    14821 cttcaaaaaa taagtaatct tctttgtttt ttccttcttt taccaacact tgcgcttctg
    14881 acgatggttc gtacgtattt tgctgtatat agtaataacc ggctccaccg agtaatccta
    14941 aaatgatgac taagccaatt agtattcgtc taaatatttt cactttctcc caccttttct
    15001 atctgagtga cccctttttc ttaatcaagt atgattatac cttaaaaaaa taagagagaa
    15061 atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat atccgccctt
    15121 tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat aaatttccat
    15181 tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa agtcaggagt
    15241 atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa aagtagcgcc
    15301 gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc ccaatagatt
    15361 tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa attcttcata
    15421 agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat ctttcttgaa
    15481 tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga tttccaaact
    15541 tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca cgacagcttc
    15601 accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa aatcaaatac
    15661 ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg catctttcgc
    15721 gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat cgaattcgtg
    15781 ttttttcaat gcttctttta cttcagaaag cttttctcca gagtaaggag ctgcatcagg
    15841 aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg gtcctgtcaa
    15901 gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat ctggcgcaaa
    15961 tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc ataaattttc
    16021 ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag catcaatcaa
    16081 agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg atccgcgacc
    16141 aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat tattttcgtc
    16201 ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc cagagccaat
    16261 ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta gcttttcact
    16321 gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat gtccttgtcc
    16381 tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg attctaatgc
    16441 ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct aatatgttaa
    16501 aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg acgttttaag
    16561 cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa cactcactga
    16621 atggagctat tgacttgtcc acgtgcttat cgatagccgt ttatcggttt cttcattctt
    16681 atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac acctaggatc
    16741 acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca ccacactgaa
    16801 acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg tgtaattaag
    16861 tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc gacgccggca
    16921 aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga agttgaagga
    16981 atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac acttgaagat
    17041 aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa tccattgttc
    17101 ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat caaatcagaa
    17161 gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt gattgctact
    17221 acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc acctaatgga
    17281 agcatgcttt tacctagaac cgtacctagt accggaaggt ttgttaacca gtttgtcaat
    17341 gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact gatgatcatg
    17401 ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt gattacttct
    17461 tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg gttgtatact
    17521 aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata tacaatagaa
    17581 atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc catcgaaatc
    17641 ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc tagacagcct
    17701 acttgagacc ctactaatac gattgctaca gctactactg tatcaaagcc tacagccatc
    17761 atgactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc gtaagtagag
    17821 ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt tcctttgaat
    17881 ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat aatacctaaa
    17941 aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt ttctttagtt
    18001 agctcccccg ctggtacacc tagcatccca ttgattggcg ccatgaaaat atcccacagc
    18061 ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc tgcatcgtct
    18121 acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc aataataata
    18181 aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg ttccatcttc
    18241 ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt ttatcaaaaa
    18301 gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa aaagttcgtt
    18361 tttccctatt ccctcctctt ttttcaggtt tttaaagctt tcttcttttt cattgtagcg
    18421 tttccttttt tttataaaca aacattttcg tcttttaaaa ggagaagaat ttcacatttg
    18481 caaagaattg attgattttt atgcataaat caaaaaaata ctcaaaacaa acaaataaaa
    18541 cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa aaagaccaag aaatttcttg
    18601 atctttttga tatttatttg actgctgtat gacgttgttc gaactgatgt tgttttcgac
    18661 ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac agcactcacg atgaatcctt
    18721 tgatcaagtt gaaaggaacg atcccaatca gtacatagtt tgctaaagac atgcccagca
    18781 tctgtgttgc gttcagccct aaaaacatta agtaaagagg cgtaataacg aaataatttg
    18841 cgatactcat aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa aatttgtttc
    18901 ttcctttatg ctgtttcttt acactaaaga aataataaat cgggaatgta aagatcgttg
    18961 ttgataagaa actagcagtg tctcctacca aattatccgg agaaaaacct gtagtcagca
    19021 gatgtaatac tgaacgcaag aacgctgttg caactccggc cgctggaccg aataagaaca
    19081 tgctgatcaa taccgagata tcactaaaat cgatcttcaa aaaactaaaa gccggcatga
    19141 tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc aaacatcgct actgcaacca
    19201 ttttctgtac acgggtgttc ttcatttgaa atcctcctgt taggactcct cttcagcgaa
    19261 ctttttgacg tttctctcca aatgaaaaaa ctctattttc cagggaaaat agagttagag
    19321 ttatttgatc aagcgtcgtc aaataagctc tatcttcttt atccagacta tactgtcggt
    19381 atcggaattt caccgattca gctatttttt tgcaaaatag gtcgtggact ataaccaccg
    19441 gtcgggaatt tcaccctgcc cctgaagacg aaccttatat aatttttgtt tacatattca
    19501 ttatacatat ctattcaaaa aacgcaacat ctaacactta taaaaagtaa gtgttcattt
    19561 tgagtggttc agcaaactgc tgacttcttt tttgttcagt tcacggtaat ccccaggacg
    19621 taggccttgt aaagtcagat ctccgtattt ttcacgtttt agtttttgca caggaagacc
    19681 aactgcttga agcatatttt tcacttgatg attacgtcct tcgtggatcg tcaattccac
    19741 gatactgtgg tttgtcttag gatccactga taaaatttgg aatttagctg gagatgtttt
    19801 tcgtccttcg atctttattc cttttgtcaa aggtgcaagc atcgattttg tagcgatccc
    19861 ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca ggcgctgagc
    19921 aaaatctcca tcatttgtca gtagcaagat ccccgaagta tcgtaatcca gtcgacctac
    19981 aggatagata cgttctttta ccatcggcaa aagatccgta acaacttttc ttcctttgtc
    20041 atctgcaaca gcagaaataa ctcctttagg tttgtacaat aaatagtaac cgtattcttc
    20101 ttgataaatt gggacttcat ccacttccac agtgtcatgt ttcccaactt ttatccctaa
    20161 ttcccttaca acttgtccgt ttacttttac acgtcctgca aggatgaatt cttctgcttt
    20221 tcgacgagaa gtgatgccag cgtgggcaat tactttctgc aatctctcca ttacaattat
    20281 tctcctttat tcattccatc aaagaataga tctggtgaca cgtcttcttc aagtgaaatt
    20341 tccatttctt gtgtatcagg cagttcttct aatgatttca aaccaaaata atctaagaag
    20401 tagccagttg ttccatataa tatcggccga cctggtcctt ctactctacc tttttcttca
    20461 atcagtttat gggcagctag acgctgtacc gctcctgaaa cctgaactcc tcgaatctgc
    20521 tcgatttcag ctcttgtgat tggctgttta taagcgatga ttgctaaggt ttcgacggcg
    20581 gcttgagata atgtgtttga tgtcccttgc gcatatcgct taagtaaggg agccagctct
    20641 tttttagtcg ttaatacata atggctttcc gtttcaaaaa tatgcaaagc tgagtgcgag
    20701 ttttgagtgt aagattcttt taattctttc actaaacttg tgacttctgc ttcgctgaga
    20761 tttaacagtc cgcttaattc ttcattactg atcccttcct cgccaacaat aaaaagaagg
    20821 gcttcaagtt cacttaattt cgtcatgttc ggtatctcct actgctggat aaagcataat
    20881 tgattcatag ttttcttttt gttgcgcaat gatctgccct tttttcatca attctagcaa
    20941 agccataaaa gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag aatcgaaagt
    21001 acagccatgc gcatttcttt ttatacgctc ttcgataaat accattcgtt cttcgatcgt
    21061 tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa aatttcttac gttctaagat
    21121 tttctgcatg gcacggatca aatcgatttt tttgacttgt cctcgcttca acggcttttc
    21181 agcatccgct aacccatcga tgttcatagg ttcttttgtg taataaagac ttcgttcctc
    21241 tgctttttct gtcagtcttt cagctgcata tttatattta cggtattcta aaagctgagt
    21301 gaccaattct tctcgaggat cttcttccca taattcttca tcgtctgtgc cgaattcttg
    21361 gacaggaagc aatgtcttgc ttttgattgc catcaatgta gcagccatca ctaagtattc
    21421 tcctgcaaca gctagctcga gtgttttcat tgtatggata taattcatat attgctccgt
    21481 gatcgtagcg atcggtatat catatatatc gatttccatt tgctgaatca gatgaagcag
    21541 cagatcgaga ggaccctcga atacatctaa tttaatattg atctccgtca actcattcac
    21601 tctttcttct ttttctttgc tagccatttt ccaatgtaag gctctgtgat ttcagttgca
    21661 cggatggaga catccgggta cttttcctgt aattcatcta acatttgaaa gcccatattc
    21721 tcaccgcgat aagacggatt caaagaaatg tcatggatga tcagctgatc gttcgttgcc
    21781 cagtaaatac caataatccc ttgaatgttg gttgaacctt ctggcgtaaa gaaaaaaagt
    21841 tcataatcgt cttcagattc gtacgtatca atctccttca acaaagaagt tttacctgtc
    21901 agtttcttat ggaaactcaa tagtcccata gcaattttac ggttttcttt ccggtaatgt
    21961 gttaacatct tctcaccttc ctatgactaa gcccttggaa aatgctgctt ataaacatcc
    22021 gtcattcttt tcttcgtgat atgtgtataa atctgagtcg tagagatatc cgcgtgacct
    22081 aacaattcct gtactgtccg caaatcagct ccattttcta gcagatgtgt ggcaaagcta
    22141 tgccgcagcg tatgtggtgt tacttctttg taaatcccag cctctcgtac taactgcttc
    22201 agatttttcc agattccttg ccgagaaaga cctttcccat gattattgac aaatacatgc
    22261 atttcagatg cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg ttccagccat
    22321 tggatcgcat aatcacctaa aggaatgatc cgctctttat ctccttttcc aactgtctgt
    22381 accagtccaa gagaaagatg aagatctccc aatttcaatc caatcaattc actgacgcgc
    22441 atcccagtag catacatgac ttccaagatt gcacgatctc ttatccctaa cgtttttgtc
    22501 gtatcaggtg cttcaatcag cttttctact tctgtcagag tcaatgtact tggtaatttc
    22561 tgtgctttct tcggcgtatc gatatgttgc atcggatcat gatcagtgat acgctcttgc
    22621 cgtaaaaact gatggaatct tctgagactt gagatcatcc gagtaacagt tgctgtcgct
    22681 tttttttctt catgcaactt ctccagaaaa cggatgacta aaaagcgatc gacatcctgc
    22741 catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc acgttcatag
    22801 ctttgtcgtg tattggcgga caatccccgt tcaatattca agtagtggag ataatcgttg
    22861 atttgctctt tcataataat ccccctcatg cctctctatc atatcagata ccgcttcatt
    22921 ctgatagtct ttttccataa tctgagcaaa gaaaaagccg tctagacact cagacggact
    22981 ggtttctttt tt
//
LOCUS       DACCXO010000037        22750 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.038, whole genome shotgun sequence.
ACCESSION   DACCXO010000037 DACCXO010000000
VERSION     DACCXO010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.038"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(59..682)
                     /locus_tag="IVY70_001326"
     CDS             complement(59..682)
                     /locus_tag="IVY70_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958463.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            872..2578
                     /locus_tag="IVY70_001327"
     CDS             872..2578
                     /locus_tag="IVY70_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP7958464.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2736..3638)
                     /locus_tag="IVY70_001328"
     CDS             complement(2736..3638)
                     /locus_tag="IVY70_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7958465.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(3635..4339)
                     /locus_tag="IVY70_001329"
     CDS             complement(3635..4339)
                     /locus_tag="IVY70_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP7958466.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(4370..5011)
                     /locus_tag="IVY70_001330"
     CDS             complement(4370..5011)
                     /locus_tag="IVY70_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7958467.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(5049..5363)
                     /locus_tag="IVY70_001331"
     CDS             complement(5049..5363)
                     /locus_tag="IVY70_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958468.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(5351..5980)
                     /locus_tag="IVY70_001332"
     CDS             complement(5351..5980)
                     /locus_tag="IVY70_001332"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP7958469.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5987..6703)
                     /gene="pyrF"
                     /locus_tag="IVY70_001333"
     CDS             complement(5987..6703)
                     /gene="pyrF"
                     /locus_tag="IVY70_001333"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP7958470.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(6700..7632)
                     /locus_tag="IVY70_001334"
     CDS             complement(6700..7632)
                     /locus_tag="IVY70_001334"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP7958471.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(7629..8405)
                     /locus_tag="IVY70_001335"
     CDS             complement(7629..8405)
                     /locus_tag="IVY70_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP7958472.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(8459..11644)
                     /gene="carB"
                     /locus_tag="IVY70_001336"
     CDS             complement(8459..11644)
                     /gene="carB"
                     /locus_tag="IVY70_001336"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP7958473.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(11649..12725)
                     /gene="carA"
                     /locus_tag="IVY70_001337"
     CDS             complement(11649..12725)
                     /gene="carA"
                     /locus_tag="IVY70_001337"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP7958474.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12730..14010)
                     /locus_tag="IVY70_001338"
     CDS             complement(12730..14010)
                     /locus_tag="IVY70_001338"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP7958475.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(14029..14955)
                     /locus_tag="IVY70_001339"
     CDS             complement(14029..14955)
                     /locus_tag="IVY70_001339"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP7958476.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14968..16251)
                     /locus_tag="IVY70_001340"
     CDS             complement(14968..16251)
                     /locus_tag="IVY70_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP7958477.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(16265..16804)
                     /gene="pyrR"
                     /locus_tag="IVY70_001341"
     CDS             complement(16265..16804)
                     /gene="pyrR"
                     /locus_tag="IVY70_001341"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP7958478.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(17039..17950)
                     /locus_tag="IVY70_001342"
     CDS             complement(17039..17950)
                     /locus_tag="IVY70_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP7958479.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17947..18411)
                     /locus_tag="IVY70_001343"
     CDS             complement(17947..18411)
                     /locus_tag="IVY70_001343"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP7958480.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(18416..19114)
                     /locus_tag="IVY70_001344"
     CDS             complement(18416..19114)
                     /locus_tag="IVY70_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7958481.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(19101..19487)
                     /locus_tag="IVY70_001345"
     CDS             complement(19101..19487)
                     /locus_tag="IVY70_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958482.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(19634..21304)
                     /locus_tag="IVY70_001346"
     CDS             complement(19634..21304)
                     /locus_tag="IVY70_001346"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP7958483.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            21487..21720
                     /locus_tag="IVY70_001347"
     CDS             21487..21720
                     /locus_tag="IVY70_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP7958484.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21819..22250)
                     /locus_tag="IVY70_001348"
     CDS             complement(21819..22250)
                     /locus_tag="IVY70_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP7958485.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            22329..22742
                     /locus_tag="IVY70_001349"
     CDS             22329..22742
                     /locus_tag="IVY70_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP7958486.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
BASE COUNT         6437 a         5076 c         3999 g         7238 t
ORIGIN      
        1 ttgttctgat aattaaaaat agcgctatat agacccaccg tttcatcgcg ccaatgtctc
       61 aattagcaat ctcggaattt tgggaagtta ctcccgttct atccaattct tcttctaatt
      121 gtttgatatt ggctaacgga agtcgattca gttcagggat caactcctta tcaaaatcag
      181 ttattcctcc cttacccgca gttatccgat ttgcttcttt tcctccatca aagaaaataa
      241 atgtcacttc atccgttcgt atgttatatt tttgttccag ttcatgtaca tcataaacga
      301 tcggataata aaagatcggt cgattcaatt cttccataag ctcaggatca ttaaaaacat
      361 caagcatgtc ctgataggtt ttcccgttcg gtttgctgaa catcaccgat atcgcccggc
      421 tttgtttgat tttctgatca gctttgctgt aatcaaaagg tttcaatttg ctggttggta
      481 tggacgaata aacgagacta tttacttgct cttctcggat tttttccggc aataaaaaca
      541 ttgcaactgt taacagcaat gataagatga gcagaaaaac aagacgtcga aaatacttac
      601 ttggattttc tgcgaatgac actgagaaat gttcattcat ttcttgtact ctcgatttaa
      661 ctacttccca aataacaaac accttctttt ctactttttc tcctattttt tattatacct
      721 aaaaatttgg atttcaacga tagaaacact gttttttatg taattagtcc aagatttttc
      781 tagacttttg ctttactatt cggccaaatt ggtttttctt ctttttttcg ttataatgaa
      841 tatacttatg aaaagaaatg aggaatgaac catgtccttt gatggtgtat ttacccacgc
      901 gatggtcaac gaattgcgtg aaacgttact atctggccgg atctcgaaaa tccatcagcc
      961 ttatgaaaat gaaatcgtgc tggtgattcg ttcacgcggc aaaaaccatc gattattatt
     1021 atctgcgcac ccaagttatg ctcgtgtaca gattacccaa atcgattatc agaatcctga
     1081 taatccgcct aacttcgtga tgatgcttcg aaaatatttg gatggcgcta ttttagaaga
     1141 catcgaacaa atcgaaaatg atcgagtgat ccatttccac tttgctaaac gaaacgagtt
     1201 gggtgatcta caaaatatca tattgatcgt tgaattaatg ggacgtcata gtacgatcgt
     1261 cttagttaat cgagaaactg gaaaaatact ggatgcgatc aaacacattg gaagctctca
     1321 aaacacttat cgttctttgc ttcctggtgt agaatatgtt gctccgccaa aacaagaggt
     1381 cttgaaccca tttagctccg agaaagaaaa aatctttcag cggctttccc aaactgaact
     1441 agatccaaaa gcaatccaac gtcagtttca gggaatcggt ttcgatatcg ctcaagagct
     1501 gaccaaaaga ttactggaac gtcctaatga aaagatggtt gtatgggacg aatttttctc
     1561 agccatcagc catcagccta ttcctacttt ttacgaaacg gaaaataaag attttttcac
     1621 accgattgct tatcaggtat tgtccgaaca ggcatctgct gtaacagctt atccaacact
     1681 cagtcaatta ttagatagct attatcacga aaaagcagag aaagatcggg caaaacaaca
     1741 aggcggagaa ctgattcgaa aaatcgaaaa tgaactaaaa cgaaataaaa ataaattgaa
     1801 aaaacgtgaa caaacactca aagaatccga aaacgctgag aattaccgta gaaatggcga
     1861 actacttact acttttctga cacaagtacc acgcggcgca aaagaagttg ttttacctaa
     1921 ttattatgaa gaagatcggc caatcaaaat agcactagat ccggcattga caccaaatca
     1981 aaatgcacag aaatatttcc atcgatacca aaagctgaag aacgctgtta agctgattgg
     2041 cgaacaaatc caagaagcca aagatgaaat ccaatatctt gaatctgtct tatctcaact
     2101 ggaaattgcc ggcccaatgg atatcgaagc aattaaagaa gaattgactt cagaaggata
     2161 cctgaaaaag aagaacttaa aaaaacaaaa acgtaagaaa ccatctcagc ctgatcaata
     2221 cttttcttct gacggaacgc tcattcttgt tggtaaaaac aatcttcaaa atgaccagct
     2281 gacaatgaag acagctaaaa aaaccgacta ttggcttcat gcaaaaaata tacccggttc
     2341 gcacgtcatc attaaaagtg acaagccctc agatgaaaca attacagaag ctgccgagtt
     2401 agctgcctat ttttcaaaat accgatactc tgcccaagtg ccggttgatc ttgtacaggt
     2461 aaagcatatc cgaaaaccaa atggcgccaa gcctggttat gttatttatg aaaaccaaaa
     2521 gacgattatc gtgacaccag aagaagaaaa aattacagct atgaaacaaa acaggtaatc
     2581 aatgagtgtt tctagaatca tatttgcgta ctttcccaca acagcaatta gttataaatt
     2641 gtttgctaca aaaagaggag ttgtgacaaa attctgtcac aacccctctt ttttgatcaa
     2701 cggtgttcaa taaacagcgt tctcatctgc ttttcttagc tctgctggtc cagtaaccga
     2761 gtaagctctt tgataaatct ttgagcgatt tcactgagcg gacgtttcgc cgaataaatg
     2821 atataaaatt catttagttc gccttctgcc aaagggatta accgcaagtg ctcgcgaatc
     2881 cctccaatca caataccaga tcctgaagcg taagcatcag tttcaagaac gatattcatc
     2941 aaagtacctc gatcactcgt ataaatcact gtttcttgat cagggatctc gataaggtct
     3001 tcataaaaat acgggaaatt atttccatcc tgtgtaaaac gaacttgagg ataagataac
     3061 aattcttctt tggtgatctc tgcgtgttca gctaatggat gtccttgcct taaaaaaata
     3121 tgcgttttga attttccaat gctgcggcca cttagctctc cttgttgtaa atatcttctg
     3181 ataccagctt gattatgctc attcatataa ataatgccga tctcactgtg ctgctctttg
     3241 acttcttcga tcactctcgc tgttgtcgtc tccatgatcc gaaattgttt gatctcgtct
     3301 ttgaattcct cgagtagctg tgcggtcaaa ggacctaaga aatcataatg ctgggcagcg
     3361 atcgaaaaat tgtctctctt ttcactcgtt agatagtgag tttctaatgt atcaagctgg
     3421 accaatattt tctcggcacg atttaagaag tcttgccctt cttctgttaa atatgcccct
     3481 cgattcgttc gagtaaacag cgtgatccct aactcggttt ctaattcttt gataccagct
     3541 gaaagacttg gctgggagac aaacattttc tttgccgctt ctctgaaact accattgttt
     3601 gctacagcga ccacgtattt catctgctga atattcattg ctcaaactcc tcgaaataat
     3661 aaatttctct tccattcaag ggctggattg gtcggttgtt cggtcgagca tactgaccat
     3721 cgaaggggag aagaaactct tggttcagtt ttcctacatg atccattaca aaccattgga
     3781 ttggcccgct ggtaggcgga gttgtcaatg agccaatata atggaaatgg cttttttgct
     3841 ccggaaagaa cacatccgga tttacccaat gctcatgttt ttctaagttc caactttccc
     3901 cattttcttg attatacagc caaccttcct gtaccattag ctggtacatg acacccacaa
     3961 ctagattctc ttttttgctg ttcatatgaa caaaatgaaa ttcaatatct tcctgttttc
     4021 cgttgatgat atgttcagaa ggcaagtgga aatgaatatc tgtcaaataa tagcgttctt
     4081 tcttgaactc tacataactg agttggtcaa aaggtactaa atgaatcgtg tttttgaatt
     4141 ccttgtcagt aaaccgttgc ttttgataat gaaaggaaag cgtgtctcct tctattttgg
     4201 tttcttcccc cttggtcaaa tgaataggcg actgcaatgg aaattccgca cctctcgcaa
     4261 accaatcaca taaactcgcc caattttccg gacctttgtc tccagaatag ccccattcga
     4321 catccatatt ctttttcaac tatacttccc cctgcatctc atatcatttt tatttttctt
     4381 ctttctttac aatcgttgag acggtttcta aaaacgcctc ttttccataa aattcctggt
     4441 tcaatagttc cggaaatatc tctttaaaga aatactgaac agaaggccga tgttcaaagc
     4501 ttttaatcgt agtcactgcc tcaatgaaca tctccttata aactttttcc gggttatttt
     4561 tgatgatttc atcaaaagat tcataaagaa ggtcaacctt atctgaaatg gctaatagtt
     4621 ctccttctac ggtttcatct tttccttcga acaaccgacg gcgataaact tcttggaact
     4681 cagctggaat ctcttggttg atgaattcgt ccgtcatctt ttcttcaact gtttgcagca
     4741 tccctcttaa ctctttgctt gcgtatttca ccggcgtttt gatatcacca ataaaacgct
     4801 ctgtgtagtc atgattcaat gatttttcat agagcagttt ccaatcgatc ggttgtccat
     4861 tcgcttcttc aatgtctcct agaacttgcg caatagatgc tacacgatac gaatgtgctg
     4921 caactgtatg ctctgtatat ttaaaaaatc caggtgccct tgtgatcgtc tctaaattgt
     4981 tcaatcctaa aataaattca ttcaacccca taaaagttac ctccctttga gatttctttt
     5041 tccgaatatc atatcattaa ataactgata ttcgcaacat ttccgctctt taccattttt
     5101 gaaacagctc ccttaccaaa aaatagagaa aatctatata taatagaaaa aagagaagag
     5161 attattctct tctttctgct aaccagtctt agaaaaatga attttaaaaa aatcaaaaac
     5221 tgatcacgat agaagtttct tttcatacct gatgaaaaga tgcttctcgc aatcagtttt
     5281 tggatttttg ctattccaat tgatgattct ggagacacaa atcggatttc tctatcttca
     5341 tcaagcgcgc ttaaatctgc caattttcag gatctttttt ccattctttt agcagagtta
     5401 attcttgctc atcgatataa cgatcttcta aagctgcttc aatcaataca gaataattgg
     5461 tcaacgtcat aagagggatt tcggcttttt cgaaatttgc ttttcctttt ggcaactcat
     5521 acgtaaagat agccgcaaca ccaaggatat ccgcaccttc tctttttgct gcttcagctg
     5581 cttccaagac acttccgcct gtagaaatca aatcctcgat cacaaccatc ttctgacctt
     5641 caaatatacg tccttcgatt tggttacctt tcccatgatc tttcgcctta cttcgaatat
     5701 agaccattgg caaatccagt atttccgcta cccatgcggc atgagggatt cctgctgttg
     5761 cagttccagc aatcacttgt acttcagggt aagcttcttt gattttgctt gctaatcctt
     5821 ttgcgatctc ttttcggact tttggatagc tcatcgtgat tcggttatca cagtaaattg
     5881 ggcttttgat tccacttgcc catgtaaaag gattacttgg atttaaaaat actgcttcaa
     5941 tttctaataa gtcttttgcg atactttgtt caacattcat tttaggttag ctccattctg
     6001 tttgaatcgt ttgatatgct ttataaggat tagttgactg agtgattggt cgacctataa
     6061 cgataaaagt tgagccgatc ttttttgcct gagaaggtgt gactactctt gtctgatctc
     6121 ctgcgactgt tccttctggt cttatacccg gagttaagca aacaaattcg tctcctgcca
     6181 tttctttgat cgcttctact tcccaagcag aagaaaccac accatctaat cctgcttttt
     6241 tggcacagct agcataatgg atcacacttt tttctaaaga aacttcgatt agttgatcgg
     6301 catgcatttc ttcttcactt gtagaagtaa gctgagtgat agctagaagt agcggacgtt
     6361 ttccaccttc tggagtcccc tcttctaatc ctcgcattgc cgcctccatc atacgaatac
     6421 cgccagcggc atgaacacag gtgagatcaa caccaagctt tgccaatccc cgcattgctt
     6481 tttcgaccgt attcggaata tcgtgaagtt tcagatctaa aaatacatta tgaccagcct
     6541 cttttagata acgtacgatt tcaggtcctt cttggtagaa caattccatt ccgattttca
     6601 caaacaattt ttcttctttc gggaaaaatc gaagaaagtc ttctacctct tgcctagttg
     6661 aaaaatctaa tgcaataatt ggtcgttggt ctagttgatt catcgttcac gttcctctct
     6721 cacttctttg attaatcttt ccagcgattc gatgcctagt tcgctcattc gcacagggag
     6781 ctctttaatt agtttaggac agatatacgg atcggtgaaa ttcgcggtcc ctatagctac
     6841 agcacttgct ccagccataa acatctcaag aacatcatcc actgtctgca cgccgcccat
     6901 cccaatgatt ggaaggctgg atatacttgc tacttggtga atcaaacgaa tagcgacagg
     6961 cttgattgct ggtccggaga gtccacctgt ttgattggct aaaatcggtt ttcttgtctt
     7021 aaggtcaatc cgcataccaa gaagagtatt gatcatcgta aacccatctg cccctccagc
     7081 ttcgattgcc tgagcgattg gaacgatgtc tgttacattt ggggacaatt tgacatagac
     7141 aggaacagaa gaaactttct ttactgcttg tgttaattga aaagcaacat cagaatctgt
     7201 tccaaaagca attccgccat gtttgacatt tgggcaagaa atgttcagct cgattgcttt
     7261 cacatttggt gcttcgccaa ttttgctaca aacttctaca tagtcttctt cacaagcacc
     7321 agcaacattt gcgatgatgg gtaactcatt gtactcctct aattctggaa gaagtttatg
     7381 cataacaacg tctagaccag gattttgcaa accaattgca ttcaacatgc cagatggtgt
     7441 ttccgccact cttggcgtgg gattgccaaa gcgggcattt ggagtcgttg ccttgaccat
     7501 gatcgatcca agctgattca aatcatagta ttttccgtat tctttgccaa aaccgaaaca
     7561 gccgcttgcc ggcatgatag gattttttaa ttctaggcca ggaagtttga ccgctaaagg
     7621 attctcattc atataattac ctctcctatt ttaaaaatcg gtccttcgtc acaaactttc
     7681 acactttttg ttcctgtttc atcttccggc gtatgacaaa cgcaagcata gcaagcccct
     7741 atcccacatg ccatgcggga ttctaatgat aactggacat tttgatgcga agaaaacagc
     7801 tgttccactg tcttcaataa cccgtttgaa ccacatgcaa aaacagctgt aggttccgtc
     7861 ttcgttccta acaataaatt accgacattt ccgtgcatac cgtatgttcc atcgtcagta
     7921 gcgattcttg tttctcctag acgattaaat tctttttcat aataaacgac ttcatgtgtg
     7981 gcaaaaccta aaaaatgcgt tggcttaatt cctttttcga ctaactgttt tgataactca
     8041 tatagaggag ggacaccgat tcctccaccg attataaata cttcgtctcc tgtatgaaga
     8101 ttggacaaat caaaaccatt gcctaaagga cccatgacat ctaaatggtc tccggcagaa
     8161 agagtagaga agactctagt cccctcacct tccacccgat aaatgatacg gcaagtattt
     8221 gtttcgtggt cgatatgatt caagctaatc ggtcggcgta atagcaagtc tgcacgcggc
     8281 acgagaatat gcaagaactg tcctggttgc tgcatttctt ttactagatc acctttcaat
     8341 gtcatttcaa aaatctttgg tgccagctgt ttttgctgaa cgatttccat catttcttgt
     8401 ttcatctgtc ccctcctgca acggtttttc gtctttttgt cttaacatgt gctcgccgtt
     8461 aaatagggtt agttgaaaaa gcacgggatt ccatcactcg aacgatcgca tctgctgtgt
     8521 ctaaagaagt aaacaatgga ataccatgtt caactgcttc tcttcggatg atgaaaccgt
     8581 ctttagaagc atttccccga tctttatcaa tcgtattgac aaccacttgt gttttaccat
     8641 ctcttattaa gtcgactaca tttttttctg ctgcttcctc agaaattttc gctactgttt
     8701 caacgaccag accatttttt tcgaaataag cagctgtatg tttcgttcct atcagactat
     8761 agccaatttc tgtaaaacgt ttagctaatt ctagtgcttc ttcttttgtt tcgtccgcaa
     8821 tcgtaaataa tacggctcca tattcaggca agtgtaatcc agaagcttcg aatgccttgt
     8881 acaaagcttt gtctaattgt ctgtcagatc ccattacttc tccagtcgat ttcatttcag
     8941 gccctaagta agtatcaact ttctgcaatt tcgtaaaaga aaagacaggt gctttaacat
     9001 gaacttcttt tgtttctgga taaagtccgt tttcataacc aagtgtcttt agtttctctc
     9061 ccaaaatagc tttcgtcgca atttgtgcca ttggaattcc tgtcacctta cttaagaatg
     9121 gaacagtccg acttgctcga ggattgactt cgatcacata aacctcattt tcatggatga
     9181 caaactggat attcatcatc ccgacacaat tcaaacctaa tgctaagcgg atcgtgtaat
     9241 cttcgatcgt tttcttgatc gattcagata atgtttgagg tggatacaca gccattgaat
     9301 cccctgaatg gacaccagca cgctcaatat gctccatgat ccctgggatg agtactgttt
     9361 caccgtcaca aatcgcatcc acttcgcatt caccaccaag caagtaacgg tccactagta
     9421 ctggatgttc tggagatgct ttcacagcat gttccatgta atcttctaaa tctcgctggt
     9481 tttcaacgat ttccatcgca cgtccgccta atacatagct tggacgaaca agaactggat
     9541 aaccaattct cgaagcgata accacggctt cttctttact tgtagcagta tcccctaaag
     9601 gttgaggaat atccaactct ttcagcgctt gctcgaataa atcacgattt tccgcacgat
     9661 ccaagtcttc gatcgttgtc cctaagattt ttacaccagc agcagtcaaa ggttcggcta
     9721 agttgattgc tgtctgacca ccaaattgaa cgattactcc tatcggctgt tctagctcga
     9781 tcacattcat cacatcttcg aaggtcaatg gctcaaaata taatttatcc gaaatagaaa
     9841 agtcagtcga aactgtttca ggattgctgt tcatgatgat tgcttcgtat cctgcctgct
     9901 ggattgcttt gacagaatgc acagtcgcat agtcaaattc aaccccttgt ccgatacgga
     9961 taggaccaga tcccagtact agaattgagg gcttttctga tcgtgaactt tcattttcat
    10021 attcatagga actataaaaa taaggtgttt ttgattcgaa ttccgctgca caagtgtcaa
    10081 ccatcttata tacaggaatc aaatcatttt cttttctcat ctggcgaacg gcttcttcgt
    10141 ttatttccca cagctccgcc acttttttgt ctgtaaaacc gttttgtttc gctgttttca
    10201 atatcttgtc atccaacggc tgctgtttca actgctgttc gatttctacg atatgcaaca
    10261 gtttatctaa aaagaataaa tcgatttttg tcaaatccgc taattcttct atggtgtacc
    10321 ctcttctaat agcttcagct acataaaaca ggcgatcatc ttgtgctcga accacttttt
    10381 ggattaggtc atcgtccgtg atgtctgcca actcaggaag ttcgatatgg tgtgtaccga
    10441 tttctaaaga acgcacagct ttcaataaag actcttcgat attccgaccg atagccatca
    10501 cttcaccagt tgctttcatc tgcgtgccta aaagccgttc tccgttttca aacttatcaa
    10561 acggccagcg aggaatttta gctacaacat aatctaatgc aggttcaaat tctgcaaaag
    10621 tcgttcctgt aacaggattt ttcatttcgt ctaatgttaa tccaagcgct attttagcag
    10681 ctagcttagc gatcggatag ccagtcgcct tacttgctaa cgcagatgaa cgagaaacac
    10741 gcggattgac ttcaatcaca taataattaa aactatgtgg atcaagagcg agttggacat
    10801 tacagcctcc ctcgatctta agtgctcgaa taatcgaaag agaagcatca cgaagcattt
    10861 gatattcatg gtcagacaat gtttgactag gggcaaatac gatagaatca cctgtatgga
    10921 tacccaccgg gtcaaaattt tccatattgc agacaacgat tgcattatca gccgaatctc
    10981 tcatcacttc atattcaatt tctttaaaac ctgcaatact tcgttcaatc aaacattggg
    11041 tagcggggga aagacttaat ccattttctg atacgatacg taattcttct tcattttcac
    11101 acatacctcc accagttccg cctaaagtaa aggctggacg aacgatgaca ggatagccga
    11161 ttttatttgc aaaatctaca gcttcttcta ctgtattgac gatttcagac tcggggattg
    11221 gctgattcaa ttctttcatc aactgtttga atagatcacg atcttctgct tgatcgatcg
    11281 cagaaagctt tgttcctaga agttctatgt tcaattcttc taaaatacct gatgctgcca
    11341 gctccattgc catattgagg cctgtctgtc cgcctaaagt tggaaggaga gcatctggac
    11401 gctcttttcg aagaatcctt gatacaaatt caagtgtgat cggttcgata tatacttgat
    11461 ctgccacttc tttatccgtc atgatcgtcg caggattaga atttactaaa accacttcat
    11521 atccttcttc ttttaaggca aggcaggctt gtgttcctgc gtagtcaaat tctgcggctt
    11581 gaccaatgat gatcggacca gatccaatca ccataatttt cttgatatct gtacgtttcg
    11641 gcatctgttt actgctcctt tcctgcgtcc atcatttcca taaattcatc aaataaatgt
    11701 agtgcatcat gcggaccagg ggcagcatct ggatgaaact gcacactgaa agcgggataa
    11761 tctctgtgtt tgactccttc tactgttccg tcatttactt ctacatgagt tacgatgagt
    11821 ttttctggat caatcgtggc ttcatcgact gcatagccat gattttgaga agtaaaatca
    11881 attcttccgg tcgctacttc tcgtaccggg tgatttaatc ctcgatgtcc gaatttcatt
    11941 ttataggtat ctgcaccgtt ggctaaagca aataactggt gaccgagaca aataccaaaa
    12001 atcggtactt ttccttggat ttttttgatc atttcgattg cttctggcac atcttttgga
    12061 tcaccaggtc cattcgtcag catgactccg tctggtgata gcgctaaaat cgtctcggca
    12121 tctgtgttat agggaagtac tgtgaggtta cagtttcttt tactcaattc tcgtaaaata
    12181 ctatgtttca agccgaagtc aatgacaatg actttccgac ctgttcctgg acttggataa
    12241 ggtttgttag ttgagacttg ctgcacttga ttggttgcta gaacggttgc tttcagctga
    12301 tcaaatgcat gttcgaagga atctccagca tcaactatac tagctttcat cgttccgact
    12361 tcacggattt tgcgagtaag ggcgcgcgta tcgatacctg atattccagg aattcctttt
    12421 tgtttcaaaa atgaatctag cgtcatttgg tttcgccagt ttgaagctac acgtgcatgt
    12481 tcttttacta ctactccttt gcatgttgga gaaatcgatt cataatcatc tctatttacc
    12541 ccataattac ccaccatagg ataggtaaat gtaatgattt gaccgttaaa actttgatca
    12601 gtgatagttt cttgataacc agtcatactt gttgtaaaaa cgatttctcc gaagacacta
    12661 gcatctgcac caaaagcctt tccttcgaaa actgttccat cttctaatat aagcaaacgt
    12721 tccatacttt tattctccct tctgccacac aagttttccg ttgacaaaag tcatttgtgt
    12781 ttcgcctttt actttccagc cgataaaagg tgtattctcc ccttttgata aaaagtcttc
    12841 tttatcgatc gtgcaagttt gtgtaatatc aaaaactgct acatcagcgg gggcaccaac
    12901 cgttagcgtt cctgcattca aaccaaaaat ctcagccggt ttaactgcca accaattgat
    12961 cacttgttcc aacgtgaatc gaccagtttc tacaaaatga gtatagatca gttgaaacgc
    13021 tgtttcgctt cctacgatac caaatggtga tttcatgaaa ctttggcttt tttcttctaa
    13081 tccatggggg gcatggtctg tagcgatgca atcgatcgta ccgtcaagca agccttcgat
    13141 caaagcttct ctgtcctccc gtcctcttag cggcgggttc attttccaaa agccgaaatc
    13201 ttcgggaata tcttcgtcga tcaaaataag atgatggggt gaaacttctg ctgttacgtg
    13261 gattcctgct ttttttgcgt ctctgattac acggacactt tctttagtag aaacatggca
    13321 aacatggtaa tgaacaccag ttgcttcagc tagcaacaga tctctagcaa tttgagaaga
    13381 ctcggttacg cttaagatcc cgggaagacc aagttcttct gccttcttgc cagcgtgcat
    13441 aacgccgcca aataataaag attcatcttc tgtatgggct actagggctt tattgttttt
    13501 tgcagcttct ttcatagcca gatacatcgt cccagctgtt tggacaccta caccgtcatt
    13561 tgtgaaagca aaagcacctt cttcaatcag tgcttcttga tcaactaatt tctcacttcg
    13621 cagattttct gtgattggcg catactgcaa taccttcaca actgcatctt ttctaatgat
    13681 gtcatatact tgtctcagct tttctgccgt atcaggtaca ggatttaaat tcggcatcgc
    13741 gcatacagta gtgaaaccgc cacgagcagc tgagcgagtc ccagcttcga tcgtctcctt
    13801 gtatgtaaat cctggctctc tcaaatgaac atggacgtcc actaaaccag gtgtgatcaa
    13861 ttgtcccttt gcatcaaaca cttcatcaaa ttcggcttct gagaatcctg taccgattgc
    13921 tttgattcgc ccatcttcta tccaaatttc tgctggtgtc gtcatatttt ttcgtgtatt
    13981 gatctgaccg tttttaatga gtgttttcat tttttcatcc tcctataatt acgcttttcc
    14041 atttagcact gcttctaaga ttgccattct gacgaatact ccattgctca tttgctgaac
    14101 gatccgcgac tgtaatcctt ccactaatga atcagctaat tctacatcac ggttaactgg
    14161 tgctgggtgc ataatgattg catgtttttg caacttcttc gcacgctcaa ctgttaaccc
    14221 atgttcctga tggtagcctt cttttgagaa actttctttt ccatcatggc gttcgtgttg
    14281 cacccgaagc atcatcatga catcaacttg atctagtagt tcatccaatg gcatataatg
    14341 tccgtacact tcgaattcat cgtcgtacca ttctctagga cctgagaaga atacttgtgc
    14401 gcctagacgt tttaacatct gcatattgga tttagctacg cgagagtgag tgatatcacc
    14461 tacgatggca actttcagac cttcaaaatg accaaattct tcataaatgg tcatcaaatc
    14521 aagcaagcat tgggttggat gctgtccgct tccgtctcct ccattgataa tcgaacactg
    14581 gattgtttta ctttgtatca gttcgtcata atagttctca tcaccatgac ggataaccgc
    14641 tacgtctacg cctaatgctg acatcgtcag tactgtatcg tatagtgttt cccctttttg
    14701 gacagagctt gtgcttgctt caaattcaat cacttctagc cctagttttt tctccgctac
    14761 atcgaagctt ttatgtgtgc gagtactatt ttcgaaaaag aggtttgtag caaagtactg
    14821 gcgcttttgc ggagtccaat tagcaccttg cttgaattcc tgccctctgc ggatcaagcc
    14881 cataacttct tgatcagtaa gtgcctcaac ggtcaataaa tgctttaagc tgatacgttc
    14941 tgatttgatg atcataatgt ttcctcctta gttttcttcg cttcgtgaag tttctggtaa
    15001 gataagattt aaaatgatgc caagtacagt tgctaatgcc attgccgaca atgtaaatgt
    15061 gcctacttca aagacgagtc cgccaattcc tactactaag ataactgaag caatcaacag
    15121 atttttcttt ttatcaaaat cgattttgtt ctcgattaaa atcttcattc cacttgcggc
    15181 aattactcca aataagataa aactgattcc tgagatgact ggtccaggga tacttaaaat
    15241 caaagcgctt aattttccta cgaatcctaa aatgactgca aatactgcag ctccaccgat
    15301 cacaaacacg ctatggacac gtgtgatagc cagaacacca atgttttcac cgtaacttgt
    15361 tacaggagga cctccgacaa aggctgccac gatttgtgct aatccatcac ctgtcaacgt
    15421 cttatgaaga cccggttctt caaagaaatt acgtttagtt agtttattca ataccataag
    15481 atggccgata tgctctgtca tcgtcacaaa agcgatgggt gccattgttg tgattgctcc
    15541 aagatatagc tttggttcat attgtacaaa gggaatctca aagttaggaa cagcaaacca
    15601 ggcagcgtct acgattggct tagtatccac gattccaaac agcaaggcta cgatgtatcc
    15661 gctaataata cctagtaaaa tcggaatcaa tccgagaaaa cctcttagcc acatgttgaa
    15721 gaaaattgtt aatcctaatg tgatcagcgc tacagcaata tacttgaaat catacgtgcc
    15781 ctgattatac atagcatttg ttgctgcatt tgccgcaagt cccaacccga taaccatgat
    15841 gactggaccg acgacgattg gaggcaagat tttatccagc catgctgaac cgatcttttt
    15901 gatgatcagc gcaacaatca aatagactaa tccagttgtg actgctcctt gtgcaatcgc
    15961 tggataacca tctgttttca tcaaaagctg catcgctgca atgaaagcga agctgcttcc
    16021 taagtacgca ggtatctttc ctttagtagt aaataagtac accaatgtac ctagtcctga
    16081 actgactagc gcaatacctg gatcgatacc tactaagatg gggactaaca cggttgctcc
    16141 gaacatcgtg aacaagtgtt gtaaacttaa tcctacccaa tgtcctgggg ttgggcgatc
    16201 gtggatatct aacaccacgt catcattatg aaatcctttt ttctccgaca tgtttctctc
    16261 tcctctaatc ttcctctttc aaaatcatga tgcgatcttg tccatcaagc tcttgcatct
    16321 ctactaaaat ctcttccgcc cgagatgttg gaatgttctt cccaacataa tctgcacgga
    16381 ttggcaattc tcgatgccca cgatcgacca ggactgctaa tgaaattttt cttggtctgc
    16441 caaaatccat tactgcgtcc attgctgctc gaatcgttcg tcctgtatac aaaacatcat
    16501 caatcaaaat aacttctttt ccttctaagg aaactgggat gtcagaagaa tgaagttccg
    16561 gttcttctgg attttctttt ttgtcatctc ggtataaagt gatatccagt tctcctacag
    16621 gaatatcgat atcttctagc tgcttcaggc gttcggcaat tcttgatgca atgtagattc
    16681 ctcgtgtttt gatacctacc aatacgatat cttggatact atggtttctc tcgataatct
    16741 cataagtaat tcgagtcaat gctcgcttca tggttacctg atctactact tcttttgctt
    16801 gcattttcat tccccctact tttttgcata aaaaaactcc tgccctaaga ggacaggagg
    16861 aatgtttaga ctgagcgtat actgatacac tgatagctat actatgtcaa tgagcaatct
    16921 acttatggcg caccatacgt atccttgctc ccacccttct tagcctcacg ggactaaact
    16981 taaaggaatt ctattaagtt ttatgctttc gttgttaatt actataccgt tatctaaatt
    17041 aaaatgcaat gttttttctt aatttttcta aagttttttc aaaaacttct ggaagaggtg
    17101 cttcaaagat catcttttcg tttgtagtcg ggtgcgtaaa acctaacatt tgcgcatgga
    17161 gaaactgacc attgcctttc aaagttttct tcggtccgta tactggatct ccagctaacg
    17221 gataaccgat atacttcata tgcacacgga tctggtgtgt tcttccggtt tctagtttta
    17281 attcaaccag agtaaatcct tcgaaacgtt ccaatacagt aaaatgtgtg acagccgact
    17341 tcccatcttc tcttatcgcc tgcattttcc ggttttcctt cgaacggccg attggcgcat
    17401 tgatggttcc tttttcgtga ggaatctctc catggactaa agcaacatat ttgcgcaaag
    17461 aggtcttatc ttttaattgc tgagctaatg attcgtgtgc ctggtcattt ttggcaacca
    17521 ttagcagtcc ggaagtatct ttgtcgattc ggtgaacaat acctggacga ataacgccgt
    17581 tgatcgtaga aagatccttg atatggtaca tcagcgcatt gaccattgtg ccatgcgtat
    17641 gacctgctga aggatgaacg accattcctt gaggtttgtt tatcaccgct acatcttcat
    17701 cttcgtaaac gatcgtcaac gggatatttt ctgggaccac atctaatgtt tctggttcgg
    17761 ggatcaagac agtaatctcg tctccttgtt ttactttgta atttgctttg actggctgtc
    17821 cattgacaga aaccgctcca tttttcagcc acagctgaat ttgcgaacga ctgtaatcgg
    17881 tgaataaatt agtcaatact ttatcgatcc taccagattc atggtgaatc gtcgttttaa
    17941 gttctgtcat ttttttcttt gatccttttc atctaaaata atataaatta acatacaaat
    18001 cactccacat acaagtgtca catctgctac gttaaaaatc ggaaagctca taaaatctgt
    18061 ctggaacata tctactacat agccaagacg aatacggtcg ataaaattgc caatcgcacc
    18121 agctagaatc aaactcaatc caatcatgaa ccagcgatta cctttttcac gatttttaat
    18181 catcaatgtg agaatcacaa cgactgcaat gagtgtcact atagtaaaga accacatctt
    18241 cccttctagc atactccatg cggctccagt gtttcgaata tgattcagtg acaaaatacc
    18301 tggtataaga tttttcgtct ctccaagcgc tagattatca gtgatccacc atttgaccaa
    18361 ctgatccact cctactagca aagcgctgaa tatccaataa ataattaaca aaaaatcaat
    18421 ccttttctat ttgtttataa atatcacgag gtcgtacgat cccgagcaga ttaccatcgg
    18481 gattcccatc tttagtgata agtacggctt ccaaagtccc ttgctcacga aacatcgctt
    18541 caacctcgta aatatgtgtt ttagctggaa caaaatgata atttgtgtac tttccgtttt
    18601 ggatgatcaa atccaacgct cgtttatttg ttaaatcaac cggacctttt tggctttctt
    18661 ttgctagcca aaacccgata ttacgtaaag taatcaatgc tacaaatttt cctttattgt
    18721 ataacggaaa ttgtgaatac cttttttctg ctactatctt taacagttca agtaacggaa
    18781 gatcttgttc aaaacctgtt acacgttttg caaatcgagg cagcacggtt tcaggctgaa
    18841 tcagttcttg ttcgatcagc tggattcgct tgactgtcca atcatttggc tctgcaatga
    18901 caaaatcgac tgcgatccga tcatggacga tcgcattcct taactgagca agctgtaaaa
    18961 gatcatcttc gtattttctg atcattaatt gttttctttg agctaggcgt ctgacaagtt
    19021 cggtaaatcc catatttcgt gcatttccca tctcgtctct catccatttt tcgattcgat
    19081 tgaaactgat taaaaaaaga tcagagtttg ccataatttt cctccttgat caaatatgcc
    19141 cggtttttta tatagaaaaa gaacgcaatc aacacaataa ctgcaaaaaa actgaaaaat
    19201 aaattttgta ggaaaacggc ccccaaccca gttgctagaa ataaaaacgg atagatttga
    19261 agaatcaagt aattcattgt gatcacataa gcaggctttg cttctttcac tgtataaacg
    19321 gggcttttta gccaataaaa ggtgacctgc atcgtaaagg gcacttccag atcaagcgtt
    19381 atcttttgtt catgactgag aacactgcgt ttttcattgt tgattcttag ctgaatcgag
    19441 ctacccatac cgtaaaaacc ggtttttctt ataaatgtcg ttttcatcat accctcctat
    19501 tatgctttag tatagctcaa aatacaataa agaaaaagaa tgatcctagt gtaaacaaaa
    19561 ttaattccct atcgtctaaa aaaagaagct gtagtcaaaa tacagctcct tcgatggtag
    19621 attataatca agcttaaaac aatcccaaca cttctccgtc atttgtcacg tccatattca
    19681 atgcagcagg ttttttaggc aaaccaggca tcgtcataac atctccagtc aaggctacta
    19741 aaaatcctgc ccctaatttt gggatgattt cgcggatcgt gatggtgaaa tcttccggtg
    19801 cacctagcag tgaaggatca tcagaaaatg aatactgtgt ttttgccata caaatcggca
    19861 gtttgtccca tccattcttc gtaaactctt ctatttgttt ttgcgctttt ttgctgaaaa
    19921 caacaccatt tcctccgtaa attttcttga cgattgtttc tgtcttttct tgtatcgttt
    19981 cttctaggcg gtataatggc ttgtaattag cttcttttct atcaatcaat cgtactacag
    20041 cttttgctaa atcaacgccg ccttctgctc catctgccca tacactcgct ctcttggcaa
    20101 gaatcccctg attttcacac aagtgctcca acagtgtgag ttcattgtct gtatctgtta
    20161 cgaattcatt gattgcgacg atcacaggta gctgatattg ttccatatta cgtatatggc
    20221 gctttaaatt tgcaaaacct actttcaaag catcaagatt ctcattttta agttcatctt
    20281 ttttcattcc gccatgcatt ttcaatgcac ggacagtggc gacaatcacc acagcatccg
    20341 gcgctttttt caagttaggt actttgatat ctaaaaattt ctctccgcca agatctgcac
    20401 caaatcctgc ctctgtgaca acgtaatctg ctaatctcaa tgctgtcttg gtagctaaaa
    20461 tactattgca gccatgagcg atattggcaa acggtccccc gtggacaaaa gctggtgttc
    20521 cgtaaattgt ttgtacaaga ttcggcttga ttgcatcttt cagcagtaat gccagagcgc
    20581 cttctgcttt caagtctcca gcagtcacag gctgacggtc aaatgtatag ccgacaacga
    20641 tccttgcaag acgagccttt aaatcttcaa ggtctgccgc aagacacaag atagccatga
    20701 tttcgctggc aactgtgata tcaaacccat cttctcttgg aacaccttgg attggcccac
    20761 ccaagccaac aattacttta cgcagttcac gatcattgag atcgactacc cgtttccaaa
    20821 tcactcggcg ggcatcgatg ttcaattcat ttccttgatg aatatgatta tctaaaagag
    20881 cagataatgc attattagct gttgtgatag catgcatgtc accagtaaaa tgcaagttga
    20941 tatcttccat aggcaatacc tgtgcatatc ctccgcctgc agcaccacct ttgattccca
    21001 tcacaggacc aagtgagggt tctcgcaatg caataacaga ttttttattg attttattga
    21061 gcgcgtctcc taaccctaca gtgatcgttg actttccttc gccagctggt gttggattga
    21121 tggaagtaac taagatcaaa tgcccctcag cgttatcctt tgatcgattg attccagaaa
    21181 aattgatttt cgccttatat ttcccataca gttccaaatc gtcttcctta agatctaatt
    21241 tactagccac ttcaacaatc ggttctagct ctgtttcttg tgaaatttgc agatcattct
    21301 tcatttgatt accccttttc acgaacttta ttcaacaaaa tatcttcttt gttaagatat
    21361 gccaattata ccttttttta ttcagaaaaa aaacataaaa ttacaggttt ttaatttatt
    21421 tgacgtactt ataaatactc tttataatga aggctatgat cagactttag aaaggtcgtg
    21481 agtgctttga ccacaggaat tgtaaaatgg tttgacaaca aaaaaggata cggctttatc
    21541 agttatgatg acacagaaga aatttttgta cattttactg cgattgaaga agagggattc
    21601 aaaactttgg aagaaaatca agtggtcgaa tttgagatca tcgaagggaa tcgaggaaca
    21661 caagctgccc atgtcaagaa aggcaacgtt cagactaccg atactagtat gcatgaatag
    21721 ataaacaaat agagtgagac gctaaacggg atgaaccgta cgtctcactc ttttcttttc
    21781 ctgtaaaaat tcgttttttt aggttctgat ttttaaaatt agctttttct ttttttcgta
    21841 taaggataag gaattgattc tgatttgggt ccgttttttt caaaatgatc aaaaaggact
    21901 acttgatctt tttttcgcat catgaaaaca gatggggctt tctcatcttg caacatcatt
    21961 tgcaccccag tattagttgg cgtcaaagat cgaatctgtc cgacagatat ttctcggtct
    22021 ttccaaaaag cagcataacg tataatcaca gagtttgatt ttaaaataaa agaacgccgc
    22081 aaacctagaa atagaaaaac aagaaaaata cctaaaacaa aattgctttt ccaataagga
    22141 cctgtgtttt ctaaagaaag aatcagacta aaaaacagaa tgatcaaggt caatgaccaa
    22201 tagatgatcg tatgtgccag ttcaggctgc catcgaatat tttttttcat tctctaacct
    22261 ctcaccgatt cagtaatgat ataatcttac cataacatta gtttgaacaa aaaggagaat
    22321 ctctttttat gttaaaagta tacattgatg cctctacaaa aggcaaccct ggtcctagtg
    22381 gaggtggaat cttgattgtc tatcaaggta aacaagaaca gctatctatc ccgctttcta
    22441 tagggacaaa ccatcaagca gagttcgaag tctttttaaa aactttagaa tttctaaaga
    22501 aaaacgactt acagaatgaa acgattttct gtttttcaga cagcaaaaca ctagtttcta
    22561 cggtcgacaa aaacgcaacg aagaacgagc tcttccttcc ctaccttcaa agaatccaag
    22621 aacttctatc cgaattttct ttattgatcc tgcaatggtt gcccgaaaac aaaaataaag
    22681 gagcagataa tcttgcacgt caggctctcc ataaacaatt aaaagaaaaa aagactcttt
    22741 aaattaaaaa
//
LOCUS       DACCXO010000038        22518 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.039, whole genome shotgun sequence.
ACCESSION   DACCXO010000038 DACCXO010000000
VERSION     DACCXO010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.039"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            68..2509
                     /locus_tag="IVY70_001350"
     CDS             68..2509
                     /locus_tag="IVY70_001350"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP7958487.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2839..3543)
                     /locus_tag="IVY70_001351"
     CDS             complement(2839..3543)
                     /locus_tag="IVY70_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958488.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3730..3975)
                     /locus_tag="IVY70_001352"
     CDS             complement(3730..3975)
                     /locus_tag="IVY70_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958489.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(3992..4843)
                     /locus_tag="IVY70_001353"
     CDS             complement(3992..4843)
                     /locus_tag="IVY70_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP7958490.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5009..6013
                     /locus_tag="IVY70_001354"
     CDS             5009..6013
                     /locus_tag="IVY70_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP7958491.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6254..6610)
                     /locus_tag="IVY70_001355"
     CDS             complement(6254..6610)
                     /locus_tag="IVY70_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7958492.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            6978..8165
                     /gene="tuf"
                     /locus_tag="IVY70_001356"
     CDS             6978..8165
                     /gene="tuf"
                     /locus_tag="IVY70_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP7958493.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8353..10944)
                     /gene="mprF"
                     /locus_tag="IVY70_001357"
     CDS             complement(8353..10944)
                     /gene="mprF"
                     /locus_tag="IVY70_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP7958494.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(10970..11689)
                     /locus_tag="IVY70_001358"
     CDS             complement(10970..11689)
                     /locus_tag="IVY70_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7958495.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            12009..12530
                     /locus_tag="IVY70_001359"
     CDS             12009..12530
                     /locus_tag="IVY70_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP7958496.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            12589..13449
                     /locus_tag="IVY70_001360"
     CDS             12589..13449
                     /locus_tag="IVY70_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP7958497.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLCGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13556..15949
                     /locus_tag="IVY70_001361"
     CDS             13556..15949
                     /locus_tag="IVY70_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7958498.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16131..17972
                     /locus_tag="IVY70_001362"
     CDS             16131..17972
                     /locus_tag="IVY70_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP7958499.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            17996..19105
                     /gene="rpoD"
                     /locus_tag="IVY70_001363"
     CDS             17996..19105
                     /gene="rpoD"
                     /locus_tag="IVY70_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP7958500.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            19325..21424
                     /locus_tag="IVY70_001364"
     CDS             19325..21424
                     /locus_tag="IVY70_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP7958501.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            21620..22483
                     /locus_tag="IVY70_001365"
     CDS             21620..22483
                     /locus_tag="IVY70_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7958502.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
BASE COUNT         7572 a         3966 c         4695 g         6285 t
ORIGIN      
        1 ataactggaa caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt
       61 ggcttacttg aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg
      121 cggaagttta agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt
      181 agataaaaca aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca
      241 agctggtaat ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga
      301 tgtcccagca atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc
      361 acgccagctg atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat
      421 gtctcaagca cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat
      481 ttccagtatg gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac
      541 cgcagagaat gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt
      601 gtcgtcccag ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat
      661 cccagtcaag atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag
      721 tacattggaa aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc
      781 aggaaatgct tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata
      841 cgcagaaaat aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat
      901 tgacccaagt atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc
      961 aggaatgaat ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc
     1021 tatcgtagtt agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc
     1081 aatcgcatta ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta
     1141 cggattgctt cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt
     1201 aggcttggca gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa
     1261 aaagttttat cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt
     1321 gacacaagaa actgcgttga tttttcagga acaaacactt tctgaagagc tctccgatca
     1381 catgatcgaa aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc agaattttca
     1441 aatcaatggc aagaaaaaat ggattccgat ggcaacagag gaaccatctg tcattgcagc
     1501 tgcaagtaat ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc agcggctgat
     1561 gcgagggcaa atcgttctat caggaaaatc ggagtatcaa gcagtgatca atgcagttaa
     1621 tcatcgaaaa gaagagctga ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg
     1681 cgggggcggt gttcaagata tctctactcg ggaattcatg gggagtttcc atgcatactt
     1741 atctattgat tttttagtgg atgtaaaaga tgcaatgggc gctaacatga tcaactcgat
     1801 tcttgagagt gtggcaaaca agttacggga gtggttccct gaagaagaga ttctattcag
     1861 tattttaagt aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga
     1921 acgcttaggg agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg
     1981 ggaatatgcg aagcttgacc cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg
     2041 tatagaagct gtagtggcag ctacaggaaa tgatacacgt gcggttagcg cttcgatcca
     2101 cgcctacgcg gcaagaaatg gattatacca aggattaaca gactggcaga tcaaagggga
     2161 taaactagtt ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg gagcttccaa
     2221 tatcctgcca aaagcaaaag catctttagc tatgttagat attgattcag ccaaggaact
     2281 agcacaagta attgctgcag tagggttagc tcaaaacctg gctgctttgc gtgcattagt
     2341 tacagaagga atccaaaaag gccacatggg acttcaagct cgttcattag cgatctcaat
     2401 tggggctatt ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat
     2461 gaaccagcag acagctatac aaatattgga aaaaatccgg gaaaaataaa aaagcaatat
     2521 ctttagaaga ataaagcaag atttacagtt attttattta ctgcagatct tgcttttttc
     2581 tctatcagaa aatatgttta ttattagaat tttttatagt taaggttagg catcttaaga
     2641 aatcaggcaa ctccaagata tcttcaggta gaagagaagt cgcctaacat tgtaggatca
     2701 taggtgattt gttggacagg tcaagctgat atttgagcct gatgttagaa ggaatttctt
     2761 tttacttgct gtagaaatta ttatatgact aaaaaggctg ggattttaaa tcccagcctt
     2821 ttatgacaaa tagaattttt atcgatcagt aatcgctgtc gctgcaaatt gttcgatcaa
     2881 aggaatcaaa gtcaatttga ctttgtattt ccgttcgagt gctttttcga taacgaactc
     2941 attttcagta gtcatagaaa tagttgctaa tttacgaggt tcatcagcat ttttttcatt
     3001 aataactact tcatgttcat tttgttctgt gtaaaattta ggatactcag caacggcttt
     3061 tgaaaaatca gcaaaactta aagcggcatt cgtatcagtt gagcgtgtat tttcagttgt
     3121 gtccgctttt ggtttttccg ttttttcttc tggcacactg actgtttctt ctgtaactgg
     3181 tgtcgcaaca gcttgcgcag ttggttcatc gtttttcaat cgttgatcat tccagtaaga
     3241 cacgatttca ataagaagat tgtcacggaa tcgttcgaaa atcccgtttt ctcctgaacg
     3301 aattctcttt aatacagtat tgaagttgtc atcgattggc acgttgatcc gtgtatcttc
     3361 atttgttttc ccagctaatc cgatcgttaa gatttgacgg tttttgggat caagcatgac
     3421 ttgtaacggc aaaccataac gtcgcaagct ctcttcttct agagaacgga ataattgtcc
     3481 tcttacacca tccaatgaag gacggaattg atttaataat ttttgtttct gttttttctt
     3541 catatgttct ctccttcgct catacccttt cgattataag aaaaaaacag ccaaatgaaa
     3601 aggagtaatg caaaaaaact tgcgtatacg caaattacaa cagaaaaatt tgcgtatttt
     3661 cccgtttttt gaaagaaaaa taaaaaatgg aacggattat gtatatatac ataatccgtt
     3721 ccattctttc tattttttcg gtatcaaggc aaaaacaggt agttcaggtg aataaatgac
     3781 ttcatgttcg tgccagggtt tgaccattag cgaatgatcg aaccgttttt tcagttcttc
     3841 gatgatttga tcatgtgtcc agtttcttgc aggaaaatgt tggattttct ctgcatcgat
     3901 caaaaagaca aaatcggaat gaaaagtctg ggtaacttct tccgagagag aattcaagcg
     3961 ttcgattgga tccataaaat cacctttcga cttaaaattg ggtagtatct gggtcttttg
     4021 ctttgccgac tacaccatct aactgatcga tttgtttcat ttcttcttct gttaattcaa
     4081 aatcaaataa ttcagtattt tctttgattc tgcttggtgt cactgactta ggaagaggaa
     4141 ggaattcgtg ttgcagcgac caacgcaaag caacttgtgc aatcgtcttg tcatgtgctt
     4201 cagcgatttg tttcatttca ggcacatcga agattttacc agtgcctaat ggactatatg
     4261 cttcgaggat catatcatgt ttttttgcat actcaactac agttgtctgt aattcgcctg
     4321 gtgcaaggaa aacttgattg accatcggca taatttttgc tgtttttgct agtgtatcta
     4381 gatggtgggg taagaaattg ctcacaccga ttgctttgat tttacctgct tcatacagtt
     4441 cttcaaatgc tcgccatgta tctgcatttg cttgttccca attatcacga aaagcaacag
     4501 ggtttggcca gtggatcaaa aacagatcta aataatctgt ttggagcttc ttcaatgatt
     4561 cttcaaaaga agacatcaca agttcatagc tgtggtttgc attccataat tttgttgtca
     4621 agaatatctc ttctctagga attccacttt ccttaatcgc ttggccgacg ctttcttcat
     4681 tcttgtatcc ttgggcagta tcgatatggc gataacctgc agccagcgct tctttaacag
     4741 aggaaacagc gacatcccca tcaggtgttt gccatgtgcc gaatcctact accgggattt
     4801 cgtaaccatt tgatagacga taagtgcttg ttaatgaatt catactttca gctccttttt
     4861 tattcgttca actataagtg tagcgcattt ttcgaaaaaa agaatccatt ttgtttctac
     4921 tcttgaaatt tttgtttaaa agaaaatgat tttgattctg gtatttttga ttttttttcc
     4981 gtaaactaag acagaaggga atgagaaaat ggatatctat ttaaaaaaag caattctgca
     5041 tattattgac agagaaactg gttctccagt cttttcacaa aaagagctgg atctgacaaa
     5101 agaatatatt cgtgattttc ttcaaaagaa gatccaaaaa atctcttctg ctcaaacaaa
     5161 aactggacaa ctagcagaag aaagcatgat cgttgaatca cttaaaatcg ctcaagaaaa
     5221 ttttgtggaa gcaagtgaag caatcgttca gcggtggttc gatatctacc aagaaagtga
     5281 agaagcacct agtgcagatg tatttgttgt cttgtatgaa ttagatacag tgatgtactt
     5341 atctttattg aaagtcaatt atcgtgaggc ttacactcac tttgttgaag cagacgaagc
     5401 agggatcgac aaccgcttga ttttgaatcg agcaattcta ggggcgaaaa cacaaaaagc
     5461 agatgaagca ttcgctgtga atttgactga ttggacctac gaattaattg aaaaaaaata
     5521 tgagttttct ggcaaaaaag agctttattt ttctggaaga gtgatcgaaa gtgttcctgc
     5581 accttcttta gaagaaaatg taaaagtgat ccaaaaagta gccaagcatc ttggaaaaaa
     5641 atttgagaca gaagaatttg atatcatggc tgatttgaaa gaagctgtct atgatacgat
     5701 agaagaaaaa ggtcggttgg atcatgagat gattgcggaa aaagtcttca aggaaaacat
     5761 cacggcaaaa atggctttta aagaagaagt acaagagcaa ggatttgtcc cagaagcacc
     5821 tcctgtaaaa gaagtacaag aaatttctga gaaaaaattt ggaaaacaaa aattcaagct
     5881 atcaaacggg atcgaactga tcgtaccagt agatgtatat cgcaacccag atttgattga
     5941 atttgtgaat aatccggatg gtacgatatc cgtgatgatc aaaaatgtgg atgaagtatt
     6001 gaatcgttta taaaaatgac tagaatatta ggtgttcaag taccagtgat cagttaaatg
     6061 aattgtgtca agagaaggat cggaaaagac gaatataaca aaaaaagagc gtgagacaaa
     6121 agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaag cagaagcaac
     6181 tccttcttat ttcttggagt taaacgcttc tgtcccagcc tctttttatc taataaaatt
     6241 ttaagaccat ttactaatta tcgatttttt ctgccgcatt tttaggcact tcagaagcct
     6301 ttatttcttc ccagcttaat acacctttac gaggattgac tttgagttta agatacgcat
     6361 tcatctttaa tggatgttct ctttcttcgc gaagcgtcac ctgttttttt tcaccatctt
     6421 tattataagc cgcttgcttg taggtataag tcgtgaattt ctctccgtta tctgcttggg
     6481 tagctgacat ttcgccattc gtcgtgattt ttgtataata agcttcgccg ccgtaaaaat
     6541 aatgataagc tgcaaaactt ccgccgagaa atatgcctaa aacgacaagt acgccaatta
     6601 attttttcat gtacttcacc tctttcctta ttattaacga aatagaagat ttcgtctatt
     6661 gaaagttgtt ggcaacagct gaattttaaa cttttcctca ttttgttctt attgcttcaa
     6721 aagaaagtta aggactatct tgaagttttt ctcaaaaaac acattttttt atttataaaa
     6781 ggtacttttt tcgcgtataa gacagacgag tgctcttagt tgactttgac caatattgac
     6841 gtataataag cttagttcga atcttacaaa aagtaagtcg gacgtattct ttttgattta
     6901 gtcaaaaaaa gactataata aaaaggtgag agctgaaatt tttcagtgat ctatattatt
     6961 taggaggttt gtaaattatg gcaaaagaac attatgacag aagtaaacca cacgttaaca
     7021 ttggtacgat tggacacgtt gaccatggga aaactacttt aactgctgcg atcactacag
     7081 tattaggcaa aaaaggccta gcaaatcctc aagactatgc tagtattgat gcagctccag
     7141 aagaacgtga acgtgggatt acgatcaata ccgcacacgt agaatatgaa acagaaaaac
     7201 gccactacgc gcatattgat gcaccaggac acgcggacta tgtgaaaaac atgattacag
     7261 gggcagcaca aatggatggt gcaatcttag ttgtttctgc aactgacggt ccgatgcctc
     7321 aaacacgtga acacatttta ttgtcacgcc aagttggtgt aaaatacctg attgttttct
     7381 tgaacaaagt tgatttagtc gatgatgaag aattgatcga tttggtagaa atggaagttc
     7441 gcgagttatt gagcgaatat ggtttcccag gcgatgacac tcctgtgatc aaaggttccg
     7501 cattaaaagc attgcaaggc gatccagatg ctgaagctgc tattatggaa ttgatggata
     7561 cagtagatga atatatccca acaccagaac gtgatacaga taaaccatta ctattgccag
     7621 tggaagacgt cttctcaatt acaggtcgag gaactgttgc ctcaggtcgt attgatcgtg
     7681 gtgctgttcg tgtcggtgat gaggtagaga tcgtagggat caaacctgaa acacaaaaag
     7741 cagttgtaac aggtgtagaa atgttccgta aaacgttaga ttacggggaa gctggggata
     7801 acgtaggcgt gttgttacgg gggatccaac gtgacgatat cgaacgtgga caagtacttg
     7861 ctaaaccagg ttccattact ccacatacaa aattcaaagc agaagtgtac gtgttgacaa
     7921 aagaagaagg tggacgtcat actccattct tcaacaacta tcgtccacag ttctacttcc
     7981 gcacaactga tgttacagga acaatcacat tgccagaaga tacagaaatg gtcatgcctg
     8041 gtgacaacgt gacgattgat gttgacttga tccatccaat cgctgttgaa aacggaacga
     8101 cattctcaat tcgtgaaggc ggacggacag taggttcagg tatcgttaca gagatcgaag
     8161 cataagaaac gcgactcgaa tattttggta atgaaaaatg tttaaaataa aagagcggtc
     8221 caaacaggat ttctgtttgg accgctcttt gttcttcaat tgtataagaa gagattgtag
     8281 aagcatgaac tttcgtttta cttcagtctc ttttattcat catgagagtg ttcatctatc
     8341 ggtatgcagt ttttaatact ttcttcgtat ccattttttc aaaccactta ttctaccttg
     8401 tgtctgctca actggcgcat tatcaataat cagcaaagca atcataacat atgcaatcca
     8461 actatctcta gaatatagag tataacgtga gacccagttt gtagcgtact tgcttttata
     8521 ttctcttaat ccatggaaag aataaaaatg agaaccaaac tcgtaaacca agtatgcaat
     8581 cctttcctgt aaaaaactct ttctggaagt tccgacattt gaaagagggg ccatccctaa
     8641 attgaaccat tggatatgtt cttctttcat ataggcaaac aaatgcaaga acaagaaatc
     8701 catactgcca gaaggtgctt ttttagaatc atatcgcatc agatcaatcg ttcctacttt
     8761 attgttcgtg taagtaggca tgatcgttgc aaaagcgaca atttcttctt gggtattttt
     8821 gacaactgca ataggcgctc tttgtagata atcttcagag aaaaagccta aagaaaaccc
     8881 tttttccttt cgtgaaccca accaattatc agagatattt ttgaaagtac tcatttgctc
     8941 agtagaaaat ggtggttgta agacatcaaa tgtaaatcct tctcgttcga tccgattcaa
     9001 gacagcacga gtgcccttca tttttttccc tgaagttgta aaactattca ggtctacata
     9061 agcttcttcc cccatcttaa tgaaatcgta gccgaattca tgcaaaatca taacaatctc
     9121 ttcgcttgtt tcgtagaaaa ctggtaaata gcatagtcga tctgtttctt cgataaaagc
     9181 ttcgattgct tctggaaaat cttctttctt accagaagga tcacccatta caatacattt
     9241 attattgaag caagcgaatt ggagaagaac agttggttca ccgtcttttt cataggcaaa
     9301 catccgctta tcttttaaga agatcaattg actatcggag tttcctccat aagttgtaag
     9361 gatcttcagg gccttttctt cattaaaggc ttcgccaatc tgtttctttt ctccttgaag
     9421 gaaatgaaca aatagaacga tcatgaaact aacagcgatg atggccaaca atcccgaaaa
     9481 ccaaatcttt tcagaaggaa ataagaaaaa ggaaataaaa tggtgacgcc gatgagggaa
     9541 atccggcaga ttgtataccc caataacgat gtaaagcaag gacagtgcac caataatgat
     9601 tccatcgatc gtacgccact cccatgaata gatcaattgt tcccgaaata attcgttttt
     9661 agaagcaaga atgatcagaa gcagaataga caagaaaacc accggtgtga aactaaaatc
     9721 acttaataaa acataaagta aagctagaac aatcaagaag attgtaggca aatatgctcg
     9781 tttcacacgt gctgcgatcc ctctgcccat aatgattaaa agaaatccca atagtatact
     9841 aggaaattgg ataataaaat gaaatttcaa tgggttcaaa ctatgaagcc agcgaaactc
     9901 tgtaaaagct tgcgggatag tggcagaaag tactagcatg attccagaaa agtataataa
     9961 gacaaccacg attttatgcg caatttctgt tgctaattgt ttagggatac cagaataacg
    10021 ctgatcaaaa gtggaaccga tatttttgaa gaaaaagacg attccaatca aaaacggaat
    10081 gatgtagtag aacaggcgat agagcaagat ccacacaacg actatttctc ttggcacacc
    10141 aatagcggac aacccgataa tcatcattac atcgaaactc ccaatttctc ctggaatcat
    10201 agacacgatt cctatcactg aagcagcaat gaataatgga attgtttgcc acagatcaat
    10261 ccggatttcc attaaataac caacagaaag gaaactaaac aggacaccgg tccattccaa
    10321 caatgaaact agcaatagct gtccttgagt tttaagtgag gtaccaccaa ggaatccttt
    10381 ctttttgaaa gtagtaaata aaaagacgac aggaaaatat agaccgccgc cgatcaacca
    10441 tatccaatat tgcttcaaaa aaggagcaac tggcgtgaag actacaagta aaaaagaaag
    10501 cagactgtaa atagaaagtc cagccatcaa aaacaagaaa actttcgata atgcttggat
    10561 cacgtttctg ctctctttct tttgtccata tagttcagag cgaagcccca cactaacaaa
    10621 cccgccgaaa ccggcaatat tgttgatcgt attgatcagc cagcttgtct caaacaggta
    10681 acgaggattc tgcttttgtt caagcatccg attcaaaata atatcataac caaccatagg
    10741 taacacagag ataagaccaa tcaatagcat tagtccgctt ttccatacag gtatagtggc
    10801 aaacgtttct cctaattgct ggatagatag agttttaccg atggacatca attcaccgac
    10861 aataatgaca atgacagaaa tcaaaaagat tgttttgaac agtcctaggt ggtttttcag
    10921 ccattgtatg gtatttttta acaaataaat caccttctat tcctaatagt tatgtttcag
    10981 aaagaaattg aagaacttct cgattgaagc gttcaggatc ttttctagct aattcatgtc
    11041 cttgttcttt cacaagcaca aaggaagctt ttggtatagt tttagctata tacaaagaat
    11101 gttttagctt gattacatct ttttttccca cgatgatcaa agtgggggag ttgatttttt
    11161 ttaaatcatt ttctgtaagc ccgatgtcat gcagcaaaag tttgataact aaaagatttt
    11221 tgcgaagact tgggcgaaat aaagagagta accataccca tgcataatga atattactga
    11281 tgactctggc agaaaaacga acgcctttaa caagcgtgtt tccagaattt aaaatcaatc
    11341 gatgaacttt tttagggaag ctgctggcaa ataccagtgc tagatttgct ccatcactaa
    11401 atccaagaaa atctgcctga tcgatttttt ccagcaacat gatcgtattc agatcctcgg
    11461 ccattaactg aaaattcaac atagatgctt cgtttgtcga acgaccatgt cctcggctat
    11521 ctactagata cacggtatag tagcgttcga gtacagggac ctgttctgaa aaaaagcggc
    11581 cgctgccgtc attgccatgc agtaaaaaga gagggaagcc ttgaccgctc ttttcatagt
    11641 aaatttcgct cccatctggc atttttgcat atttcttttc ttttttcaca taatcaccta
    11701 ctgtgtaaag tataacgatt atatcagtca ttgggcaatt gtttttactg aaaaacaacg
    11761 atcattttcg taagatgtca aaaaatacag gaaaattcat tttttcttaa ggcgtacaca
    11821 gaaatcaagg aagaaatagc caagaagata aagaaatagt aagataaaaa aagatgtcaa
    11881 aataactagg tgatgaaatg aagcgaaaac gattatctat tatccgtatt cttttttaca
    11941 tgatcattct gacggttatt ggtgcggtgt tgtttttatg aggaagaact tatcgttcgg
    12001 taaaacgagt ggaaagctat caaatagaaa taacaacagc tgcaaaaaat tatcatatgg
    12061 aaaactacga gaatttgatc aaggcgataa tattgaccga atcaaaagga aaagggacag
    12121 atctcatgca gagctcggaa agtctgcatg gagaagctga gatcattgat aatccagctg
    12181 acagtattga tcaaggtgtc aaatatttgg cggaaatgat agtagaagca aagaaacaag
    12241 gatgcgattt atggacagct gtccaagcat ataattttgg gaaagattat attgcctatg
    12301 taaaaaatca tggcggagaa aacacagtag aattagcaga agcgtattct aaagatgtgc
    12361 tgagcccact tttaggaaat gaggaaggga aacaataccg atattggcgg atccagtcct
    12421 tgctttacaa tggcggctat ctctatcata atggaggtaa catgttttat cctgatattg
    12481 tcaaaatgaa tgaaaaaata atagagacct atgaaaaagt ctttaagtaa aatagcagga
    12541 aatgttcaat catgaataaa attaaatgaa agaaggagtg cgcaaatagt ggaaaaaagg
    12601 caaaaaatgg aaaatcaaga agtggggtac acaaccaaag actggatgtt acggtttgtg
    12661 aagggaatgt ttatcggttc aggatttatc ttgccaggag tcagcggtgg tgcattagca
    12721 gctgtctttg gcatgtatga gcggctgatt tcttttttag cccaccttac aaaaaatttc
    12781 aaagaaaatg ttttgttttt tctgcctgtt ggtctaggcg gagtgacagg catctttcta
    12841 ctttcttttt tagtcagttt tttattaggt gcgtatgaaa caacgatatt atggtttttt
    12901 gtcggatgta tcgctggaac tgttcctgcg ttatggaaag aagcagggaa aaaaggaaga
    12961 gatacttctg atgtgatcat catggttgtt acttttgttc tggcttattt gtttttatta
    13021 tgcggcgctc aattatttga cgggcaagtc gatcagaatt ttggaacatg gctgattgcc
    13081 ggcggattga ttggtttggg tatgatcgtc ccaggattga gcccttctaa ttttttggtg
    13141 tatatgggat tgtataaagc gatggctgac ggatttaaag aagtacgact tgaagtgatt
    13201 gtgccgatcg gaattggtgg gattctttgt gttcttggat tgtcaaaaat catggattac
    13261 attttctcaa aggcatatag caagctattc catttcattt taggtgtagt ttttgcttca
    13321 acgattatga tcattcctac cgattacgcg ggattgggag ttgctggggt actagcttgt
    13381 cctgttcttt ttatagccgg tacagcacta ggttggtgga tgagccgttt ggaggaacaa
    13441 tataagtaaa gatagattga taataaaaac agcacacggg tgattcaaac aaaaaggttg
    13501 gcatcttttt tgtaaaatca ttcgtgtgtt tttctataaa ttttataaaa acaagttgtt
    13561 tttaatcttg gaacacgcta gacttaaaca cgtgattatt tttgaaagtt ggcggaaaat
    13621 ggagaaaaag aaaatgaatg gacttacaca gcaagaggta gttcagaaaa tggcttctgg
    13681 ccagcaaaat gactaccagg aagatgcatc aaaaagtaca aaacaaatat tcagcgataa
    13741 tatattgacg ttattcaatt ttctgaactt tggtattggg atttgtctgt tatttgtagg
    13801 tgcgtattcc aacatggcgt atttagcaat tattgtcgtc aatatcgtta ttggaattta
    13861 tcaagaaata catgcaagaa atcttgtacg gaaattatcg attgtttccc aatcagaagt
    13921 actagtgatc cgggacggta aagaacaatc gatttctact aaagagctgg tattaggaga
    13981 tttggtaaaa atcggagcag gagaacagat accagccgat ttacaggtta tatctggacg
    14041 ggcagaagca aatgaagctc ttttgacagg cgaatctgat ttgattgtca aagaaactgg
    14101 agctgaattg ctttctggaa gctttttgac aagcggttct gtcatggctg aagttatccg
    14161 agtaggtgca gataattatg ctgtccgctt gactagcgag gcaaagactc acaaagctat
    14221 tcgttctgaa cttgtagatt cgattcgcaa agtatccaaa ttcactagtt tcgtgattat
    14281 tcctttgggg atcatactat ttttggaagc actgttcatc cgcgatgctt ctatcaaaat
    14341 ttctgttatt gcatctgcag cagctttgtt aggcatgctt ccaaagggtt tggtattgct
    14401 aataagtatt gcattaacga caggggtaac caaactggca aaaaaacgaa tccttgttca
    14461 agacatgtat tcaattgaaa cgctggccca tgtcgataca ttatgcttgg ataaaacagg
    14521 gaccattact gaagggaaaa tgagtatcca gcgggtagaa gcactacatc cgctgtacga
    14581 gaaagccatc ccagaaatta tggggacgta tctggcagaa agcagtgata acaacatcac
    14641 tatgcgggct atgagagatt attttgagct ttctaatcgg tatgatgtga caaacaaagt
    14701 cgcattttct tctgatcgca aatggggcgc gatggaattt ccagaactgg gagtcgtgta
    14761 cgtaggagcg cctgaacgtt tggtggaaga agatcggctt ccggacagtc taaaaacagc
    14821 ccaagcgaat ggctatcgcg tacttatgtt ggggatcgct ccaaatcagc aattgaacga
    14881 agaaaaacca gcatttgttg agccggttgc tctgtttgaa atcgatgatc caatccgaaa
    14941 aaacgctcaa gaaacactag cttatcttca tgaggaaggt gtggatctga aagtgatttc
    15001 tggagacaat ccttttactg tctccaatat cgctagacgt gcaggattac cgaattatga
    15061 tgcgtatgtc gatctttctc aaattactga agaaatggaa gtgcgagaag ctgctcatcg
    15121 ctatacggtt tttggacgag tgagtccgca gcaaaagaaa ttgctggtaa atgaattgaa
    15181 agaaagcggc agaacggttg caatgacagg cgacggtgtg aatgatgttt tggcgttaag
    15241 agaagcagat tgcagcattg ctatggcaga aggtgatgga gcaacacgtc agattgcgaa
    15301 tctggtattg ttggactctg attttactac attgccagaa gtcctgtttg aaggacgccg
    15361 agtggtcaac aatgtgacga aagtctcagg catctttttt atcaaaacga tttattcatt
    15421 tattttatcg attatatgtg ccgttacagc aattggattt ccgtttatcc ctatccaaat
    15481 cacgctgatt gatttagcga ttgaagggta tccttccttc tttttatctt ttgaacaaga
    15541 taaaagaaaa atcacttacc gttatttgcc tacagcactt attaatgctt taccaaatgc
    15601 cttgcttgta gtagtgaata tcattgcggt gtatctactt ggaaaaacat tcgcatgggg
    15661 aaatgctgaa actactactt tgatgtatta cttgctgatt gggatcagtt gcatggcagt
    15721 tgtaaaagct tgttatccct tcaacccatt gcgcatcttc ctagcagtaa cgacgatcgt
    15781 agggatatat gtagctgcaa tgctgttcca tggaattttg gaagtaaact tagggttggg
    15841 agagagcgta ggatacttct ttgtattcat gctcgtaaat attgttcttc gagtattgta
    15901 ctggaaggct gatattcaaa atcagttact gaaaagagta gcaaattgat gcttaatgag
    15961 atagaaatgt gttatacttg aaaaagcaat gaaatatgat acaatcaaac atgtataatt
    16021 gtgtccttta aaatcgcact gatttcagtt agtgcgattt tttaccggtt attctcctga
    16081 aatatcttct gttaaaccca aaagatatca atagagcagg gggtgaaaaa atggctcaac
    16141 ggattcctca agaggttatc gaaacagtac gcagtcaaac gaatatcgtt gaggtgattg
    16201 gacaatatgt ccagttgaaa aagtctggta aaaattactt ggggctgtgt ccttttcatg
    16261 aagaacgtac accgtctttt tctgttgcag aagacaagca gatttttcat tgctttggtt
    16321 gtggtaaagg cggaaacgtg tttacttttc ttcaagaact agaaggtcta agttttccag
    16381 aagcagtggt aaaagtggca gacttcgaac agataccgtt agaagatcaa tggcgtcagc
    16441 gtactatagc tgtccaaaat cctgaatctg ctacggggaa actgattgct gctcatgaaa
    16501 aagcagcgga aatctatcat cacatgctat tgcatacaaa agctggacag ccagctttag
    16561 aatatttgca aagtcgtggg ttgtctttag aattaattga agaattcaac ataggttttg
    16621 cacctaatga gcgttctttt cttcagcaag tattcaagaa tgaatcaatt tcaaatgaat
    16681 tgttagaacg ctctggattg tttgtcacgc atgatgatgg ccgtatgtct gatcgttttt
    16741 atcaaagagt gatgtttcca cttcgtaatt ctcaaggcca gacgatcggt ttttcaggac
    16801 gctggcttgc acctgaaaaa gggcaggaaa aagatcagcc caaatacttg aatagtccag
    16861 aaacagaact gttcaataaa agacaagttt tatttaatct tgataaagca agaagcgata
    16921 tccggaaaaa cggagaaatt ttgttgttcg aagggtttat ggatgtgatt gctgcatggc
    16981 aggctggtat caaaactgga ttagcttcta tggggacaag tctaacagct caacagatcc
    17041 aacaaatgga aaagctgacg aaagaattgg tcttttgcta tgacggagac aacgcaggtt
    17101 ttgaagcaac caatcgaggg attgaattgc ttcggcaaaa cagtcgatta gcactttctg
    17161 tcgtagtcgt tccagaaaag cttgatcctg atgaatatct ccgtaagtat ggagaagcat
    17221 ctttccaaga attagtggct catgggcgcg aaacagtctt tacttttaaa tcacgttatt
    17281 taaaacaagg gaaaaattta gataatgaaa aagacaagat cgaatacctc gaagaattac
    17341 tagttgaact tagccgagtt gtttcaccaa tcgaacaaga tatgtatcta tctcaactgt
    17401 caacagagtt tcagatcggc agagagacga tacaaaaaca actgcgcgat cttcgaagga
    17461 caaatcagca aaatcgtcaa gaacctcagg aaagtcaaca cactcaggtt acgagacaag
    17521 aaactgcacg gaaaaagaga cctttgacgc aagtagaaaa ggcagagcag attctattat
    17581 atcgtgcatt taaagaagtt tctgtccgta atattttaaa agaacgagaa tttcagttta
    17641 ttcatgatgc atatgcagaa gtttattttt tattcgatgc atatgtctca caacattatg
    17701 aattcaacct tgcagagttt ttagactttt tgcaagatga aaatttaagg cggttggtgg
    17761 tggaaatcga atatttaccg gtggcagaag aaagttcgcc acgagaaatc caagacttgc
    17821 tagatgtgat cagcaaatct ggcttagctg atgaaataac attaaaaaaa caaatgcagc
    17881 aagaagccag acgaactgga aacaaacagc ttgagcttga attgacgatt gaaatcatca
    17941 atttaacgaa acagttaaaa caagccaaat agctactcaa ggggggcctt cttttatggc
    18001 aaatggaaca gataaaaaaa cgtatgaagc agcagtagca gctttcatca aagaaaacaa
    18061 acccaaacga caagttgtat atgatgattt atccaatcag cttgctacac cattcacttt
    18121 gaatgcagat gaaatggaca aattgatcca aaagattgaa gacgcaggta tcagtgtggt
    18181 ggatgaaaat ggtgatccat caattcacag cttgaagagt gctgagaaaa aagctgaaca
    18241 agcaaaaaca gaagatcttt ctgcgccaac cggtgtaaaa atcaatgacc cggttcgtat
    18301 gtatctgaaa gaaattggcc gtgtctccct tttaactgcg gaagaagaag tagcgttagc
    18361 attgaaaatc gaagaaggcg accaagaagc gaaacagcgt ctggcagaag caaaccttcg
    18421 tttagtagta agtatcgcta aacgttatgt aggacgaggc atgcagttcc ttgacttgat
    18481 ccaagaagga aacatgggac tgatgaaggc agttgaaaaa tttgactacc gcaaaggatt
    18541 taagttttca acctatgcta catggtggat tcgtcaagcc atcactcgag caatcgctga
    18601 ccaagcacgt acgatccgta ttccagttca tatggttgaa acaattaata aattgatacg
    18661 tatccaacgt caattgctgc aagaccttgg aagagaacct acgccagaag aaatcggtgc
    18721 ggaaatggat cttccaacag aaaaagtccg agaaatcttg aaaatcgcgc aagaacctgt
    18781 ttcccttgaa acgccaatcg gtgaagaaga tgactctcac ttaggtgatt tcatagaaga
    18841 ccaggaagca acaagtccag cagaacacgc agcttatgag ttactaaaag aacaattgga
    18901 agatgtacta gatacattga ctgatcggga agagaatgtc cttcgcttac gttttggttt
    18961 agatgatggc cggacgagaa cattggaaga agtagggaaa gtatttggtg ttactcggga
    19021 acgtattcgt cagatcgagg ctaaagcatt aagaaaattg cgccatcctt ctcgttcaaa
    19081 acaattaaaa gatttcttag aataagcgtg gactattgac cagaagagaa atcagtaaaa
    19141 ctgtttggtt tattttaaaa gaaaaaaagc ttgagactac gcagtctcaa gctttttttt
    19201 ctataggaag gaaattcatt cattattttt aagtaaatcc taaaaataag ctattggcgt
    19261 gacgtataga atatgataga ttaaatggta gcgtaaacct agtaaaggag ccgatcgcta
    19321 agtgatgaaa aaatgtccaa attgcggatc gaaaaaaatg gagcagaata ctatctgtcc
    19381 agactgcggt catcaaataa ctgcagaaga tcagcaaata aaagaatcaa ttgaagaaac
    19441 aaatacagat gcagctatgc aggataaatc aactgaagat gcagtctttc ctgatacaga
    19501 attaaacgat ccaatcgaat ggtcggaatt gaaagattta cctctagaat ctgtaatgga
    19561 attattcgaa aaagcagaac cagaagacga agaaccacct gctcacgatc aagtaactaa
    19621 tatagagaaa aatgaacaga agaaagcaac tgttcatgag acttcggaag aatcaggaaa
    19681 gaatcaagaa gaaacgcttg aatcaaaaca aaaaagaaga gtagctgaac tgaaagaaat
    19741 cgtcgataat gaggaagaaa attcgatact atccgcttat atcaaagcac atcgtgaaga
    19801 tacaaaagaa gagcacgcga aagagctgct caaaatgatc agtgaaaaaa ttgcacagga
    19861 aaacaaagta gagccagaaa aggaacaaga aactgaaaaa gactctgata gagaaaacgc
    19921 cacaggtaaa gaaacagtaa aaataaaaga cgtaaaaacg gatatcacag aaaaaactga
    19981 agtatttact gaaaaacaat tagaaaagga aaaaacgaat aaactgccgg aagcagagaa
    20041 aaaagaaata ctcgaaaaca cagacaaaaa gtcaacagag cccgatgaaa taaatagtga
    20101 atccgatgct gaaaatcaat ctgtaagcaa agagggtact gatcttgaag caaaaataga
    20161 aaaagaagag aaccagccta agctgtttga agcagaagcg aaatcaataa caatcgtgcc
    20221 agatactgaa aaaacacatc aagaatcaaa cttagaaaaa acagatccag ataaagcgca
    20281 aaagaaaaaa tcaaaaaaag taccttatct cgttttagca gcagtacttc tactcggtgg
    20341 aggaagctgg gcttattatg accatcatca aaaagtagaa gccgaagtag cagcagaagc
    20401 aaaaagacag caacaaatgg aagaacttca tgagaaactt gctgcctttt atacagatga
    20461 tcaccatcaa tttattcgtg cagatatgat tcatcaagat ttatcaaaac taaaaaatga
    20521 tttatccagt cttaagaatg aaaaaggata ttcagagtta gaaacaactt accaagatat
    20581 ccaatctaag atccgagata tccaaaaagt gaatgagtgg ttcatagcac ctgccatcgt
    20641 cgatgatcat ctagcagatg acatcaagtt gaaagcagat aaagagatcc aagcaatcga
    20701 aacggataac acagattttg gaaaattgat tcaacaagca caaacagaag ctaaaaatca
    20761 atatgctgaa ttgcaaacag ctaaagaaaa aacagctata gtctttaaag acggaaaagt
    20821 agtggattca gcaacacgcg accaatacaa gacggcaaaa gaagcagtag aaaaagtgaa
    20881 aaatacagat ctatctgctc agttgaatga acaattgaaa caagtagata agatgctcac
    20941 tgataaagag aaaaaagaag aagaggaaaa gaaaaaagca gaagaagcga aaaaagcagc
    21001 tgaagaacaa gcaaaacaag ccgctcaagc agcagctgca gctcaagccg ctcaacaagc
    21061 tgcggtcgca caagcagcac agcaagaagc ggcatcctcg accccttcct cttcaaactc
    21121 agcgaatcag ccaatcaaat ctgccagagc aagtgatatt gccgatgcat ctaatccagc
    21181 atggacgtgg gcaccaggtg tccaaagcaa agtgattgct acatgtatcc aacgtggata
    21241 tatcgttgaa ggaggttaca gattagaaaa agccagaatc gaaaatgggg aaggctatta
    21301 taatctatat gccacttcaa caaaatctgc attgatgaaa ggcattggag aaagtgcttt
    21361 gccattttac atcgtaacca tcaactgtaa aactggatgg ttcggaggaa atgggagcaa
    21421 ttaattttag gcaaatgaaa gaggctgtga gcctgccgtt taacaccgtt tattcgaaaa
    21481 aggggagtat gacacaactg ttgtcatact ccttttttca cctttccaaa ccaaggctca
    21541 gctattcaag tagataacat tatgttatga tagcaaagaa atgtattaag aaaacctttc
    21601 actatagagg gagaaaaaaa tgaacgattt attagcaact atatttacag gtattattat
    21661 tgatgaaaat gaaaaaaact actttgtcca aaaaaacgga caaacctttg gcttaagcaa
    21721 atcagaaggt gaacacaaaa tcggtgaagc agttgaagga ttcggttacc aaaatcaaaa
    21781 acatgaaaat cggatcacta cacaaattcc taaaagccgt atcggtcatt acgcttttgg
    21841 aatcgttact gctacacgaa aagatttagg tgcgtttgtc gatatcggtc ttccagataa
    21901 agactttgtc gtatctcttg atgaattgcc gacaatgaca gagctatggc ctaaaaaagg
    21961 ggatcgcttg atgatttccc ttagagttga tgcaaaagac cgtatttggg gcagtttagc
    22021 agatgaaaaa atattcaaat ctttaagcaa atatggaaca gaagaaatga aaaacaaaga
    22081 tgtgatagga acagcatacc gcttgaaatt aacaggtacg tacgttttaa cggaagattt
    22141 ctatcttggg tttatccatc cttctgaacg atataatgaa cctcgtctag gcgagcagat
    22201 aaaaggtcga gtgattggtg tccgaccaga cggtgtactg aatctttcat tgaagccaag
    22261 agcatatgaa gctatatcag atgatgcaca aatgatttta accattttag aacgttctgc
    22321 agatcagcaa atcaacttta cagataaatc ggatccagaa gaaatcatga aagcttttgg
    22381 tatcagcaaa ggagcattta agcgcgcatt aggtaatctt ttgaaacaag gattgattac
    22441 acaagaaaac ggtgtgacaa aactagtcaa aaaatctaat tgagaatccc ttgcatgttt
    22501 ttcttaacta gcttataa
//
LOCUS       DACCXO010000039        22321 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.040, whole genome shotgun sequence.
ACCESSION   DACCXO010000039 DACCXO010000000
VERSION     DACCXO010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.040"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            25..267
                     /gene="yaaA"
                     /locus_tag="IVY70_001366"
     CDS             25..267
                     /gene="yaaA"
                     /locus_tag="IVY70_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP7958503.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            254..1378
                     /gene="recF"
                     /locus_tag="IVY70_001367"
     CDS             254..1378
                     /gene="recF"
                     /locus_tag="IVY70_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP7958504.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1375..3321
                     /gene="gyrB"
                     /locus_tag="IVY70_001368"
     CDS             1375..3321
                     /gene="gyrB"
                     /locus_tag="IVY70_001368"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP7958505.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3341..5812
                     /gene="gyrA"
                     /locus_tag="IVY70_001369"
     CDS             3341..5812
                     /gene="gyrA"
                     /locus_tag="IVY70_001369"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP7958506.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6048..6347
                     /locus_tag="IVY70_001370"
     CDS             6048..6347
                     /locus_tag="IVY70_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP7958507.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6397..6909
                     /gene="ssb"
                     /locus_tag="IVY70_001371"
     CDS             6397..6909
                     /gene="ssb"
                     /locus_tag="IVY70_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP7958508.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6935..7171
                     /gene="rpsR"
                     /locus_tag="IVY70_001372"
     CDS             6935..7171
                     /gene="rpsR"
                     /locus_tag="IVY70_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP7958509.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7314..9287
                     /locus_tag="IVY70_001373"
     CDS             7314..9287
                     /locus_tag="IVY70_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP7958510.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9293..9745
                     /gene="rplI"
                     /locus_tag="IVY70_001374"
     CDS             9293..9745
                     /gene="rplI"
                     /locus_tag="IVY70_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP7958511.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10019..11386
                     /gene="dnaB"
                     /locus_tag="IVY70_001375"
     CDS             10019..11386
                     /gene="dnaB"
                     /locus_tag="IVY70_001375"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP7958512.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12166..13458
                     /locus_tag="IVY70_001376"
     CDS             12166..13458
                     /locus_tag="IVY70_001376"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP7958513.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            14245..15108
                     /locus_tag="IVY70_001377"
     CDS             14245..15108
                     /locus_tag="IVY70_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7958514.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(15237..16859)
                     /locus_tag="IVY70_001378"
     CDS             complement(15237..16859)
                     /locus_tag="IVY70_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7958515.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            17108..17629
                     /locus_tag="IVY70_001379"
     CDS             17108..17629
                     /locus_tag="IVY70_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958516.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(17815..19545)
                     /gene="spxB"
                     /locus_tag="IVY70_001380"
     CDS             complement(17815..19545)
                     /gene="spxB"
                     /locus_tag="IVY70_001380"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP7958517.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            19710..20528
                     /locus_tag="IVY70_001381"
     CDS             19710..20528
                     /locus_tag="IVY70_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP7958518.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(20631..20828)
                     /locus_tag="IVY70_001382"
     CDS             complement(20631..20828)
                     /locus_tag="IVY70_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958519.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            20997..21575
                     /locus_tag="IVY70_001383"
     CDS             20997..21575
                     /locus_tag="IVY70_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP7958520.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
BASE COUNT         7182 a         4026 c         4701 g         6412 t
ORIGIN      
        1 aataatgaaa atgagggatg ggcattgaaa caacaagtca ttctaaatac ggaattcatg
       61 acgttgggcc aaatgttgaa agaagtcaca gttatcggta gtggcggaca agcaaaatgg
      121 tatttggcag aaaacactgt gttagtcgat ggcgaaccag aaaatcgtcg tggacgcaaa
      181 ctttatcccg gcatgatggt tgaagttcca gaagtaggaa ctttttttat ggtaaaaaaa
      241 ggaaacgacg ataatgaggc tgaataagct gtatttaaaa aattatcgaa attacgaaga
      301 gctgaacatc gaattttcca agaacctcgt catcttttta ggagaaaatg cgcaaggaaa
      361 aacgaatctt ttggaaagta tctacgtatt agctatgacg cgaagtcatc ggacaagtag
      421 tgagcaggaa ttgattggct ggactgacga tcaggcgatg attcaaggag agatcacaaa
      481 aggttcatcc actcttccat tagaaatctt attatctaaa aaaggccgaa aaaccaagat
      541 caaccatatc gaacagaaaa agttaagtac ttacgtggga caaatgaatg tcattttatt
      601 tgcaccagaa gatctttctt tagtaaaagg cagcccgcag atcaggcgga agtttttaga
      661 tatggaatta ggccagatca atccaatcta tctttatgat cttgtccaat accaagcaat
      721 tttgaaacaa agaaaccagt acttgaagca attgaatgaa aaaaaacaaa ctgatttgct
      781 ttatttggat attttgtccg aacaactagc agcgtttggc agcaaagtat tgaaagcaag
      841 agcacagttc gtcaagcgat tggagttttg ggctaattct ttgcatcagc aaatcaccca
      901 tcaaaaagag cagctggaaa tcgagtatct caccgcagtc gattctttag aaacacatac
      961 gcaagagcaa atccaagaac aatttctcgc acttttgaac caaaataaaa agaaagacct
     1021 ttttcgggga acaacgacag ttggtcctca tcgtgacgat ctttcctttt ttattaatca
     1081 gaaaaatgtg cagacttatg gatcgcaagg acagcaaaga acaactgcac taagtgtcaa
     1141 attagcagaa atcgatttga ttaaagaaga gacaggggaa tatccaatcc ttttgttaga
     1201 tgatgtgatg agcgagctgg atgataatcg tcaactccat ctgctagaaa caatcgaagg
     1261 aaaagttcaa acgttcttaa caacaacgac acttgaccat gtaaaaaata aaatgactgt
     1321 tgaacctgag attttttatg ttcaacaagg gaaactaaaa ggagaagaaa ctgaatgaca
     1381 gaagaaagaa gtttagtaga acgcgctaaa gagtatgatg ccagtcagat tcaagtactt
     1441 gaaggacttg aagccgttcg taaacgacct ggcatgtata tcggttcgac tagttctgag
     1501 ggtctgcatc accttgtttg ggaaattgtc gataattcga tcgatgaagt attagcaggt
     1561 tttgcaacga aaatccatgt gattattgaa aaagacaaca gtatcaccgt tattgacgac
     1621 ggtcggggga tacctgtaga tatccaggca aaaactggac gtcctgcagt tgaaacggtc
     1681 tttactgttt tacatgctgg aggaaaattc ggcggtggtg ggtacaaagt ttctggtgga
     1741 cttcacggag taggatcttc tgttgtaaac gcgctttcta cacagcttga tgtcaaagta
     1801 tacaaagaag gaaatgtgta ctatcaagaa tatcgtcgtg gagctgtagt ggacgactta
     1861 aagattatcg aacaaacgga tcgccatgga acaacggttc actttacgcc agaccctgaa
     1921 atttttacag agacaactga gtttgatttt tcaaaattgg ctacccggat ccgtgaactt
     1981 gctttcttga accgcgggat gcgtatatcg attgaagaca aacgagaaga agaaccagtc
     2041 atcaatgaat accattatga cggcgggatc aaaagttatg tcgaatactt gaatgcaaac
     2101 aaaaccgtga tttttccaga acctgtatat ttggaaggtg aacagcaaga tatcgcagtt
     2161 gaagtctcta tgcaatatac agacggctac cattccaata tcatgagttt tgccaacaat
     2221 atccatacgt atgaaggtgg aacacatgaa tcaggattca agactgcttt aactcgtgtg
     2281 ataaatgatt atgcccgaaa acaaaagctg atgaaagaaa atgacgacaa cttgaccggt
     2341 gaagacgtgc gagaaggatt gacagctgtt atttctatca agcatcctga ccctcaattc
     2401 gaaggacaaa caaaaacaaa attgggcaat tccgaagtac ggacagtcac tgatcggtta
     2461 tttagcgaac atttcatgaa attcttgctg gaaaacccaa gtgtgggccg gcaaattgtt
     2521 gaaaaaggat tactagcttc cagagctcgt ctagcagcga aacgtgcgcg tgaagtaacg
     2581 agacgtaaag gtgctttaga gattagtaat ctgccaggaa aattagcgga ctgctctagc
     2641 aacgatcctg aaaaatgcga attgttcatc gtcgaaggag attcggccgg cggatcagct
     2701 aagcaaggtc gtaatcgaga gtttcaagcc atcttgccta ttcgtgggaa aatcttgaat
     2761 gttgaaaaag caagcatgga caagatcttg gcaaatgaag agatccggtc tttatttact
     2821 gctatgggta cagggtttgg agaagatttt gatgtatcga aagctcgcta ccacaaatta
     2881 gttatcatga ctgatgccga tgtcgacggc gcgcatatcc gtacattgct gctgactctt
     2941 ttttatcgtt acatgcgtcc tattgttgaa gctggatacg tctatattgc tcagccacct
     3001 ttatacgggg taaaacaagg gaagaatatt acgtatgtac aaccaggaaa aaatgcagaa
     3061 gaagagttga aacaagtcgt tgcttcactt cctgcctcac caaaaccaag cgttcagcgt
     3121 tataaaggac ttggagaaat ggatgatcat caactatggg aaacaactat ggacccaagc
     3181 aatcggatga tggcccgcgt cagtgtggac gatgctattg cagcagatca gatatttgaa
     3241 atgttgatgg gggatcgagt agaaccccgt cgtgcattca tcgaggaaaa tgcacactat
     3301 gtgaagaact tagatattta atcatgaagg aggaaaaaga atgagtgaag aaatcagaga
     3361 aaacatccat gatgtcaatc taaccagcga aatgaaagac tccttcatcg attatgcgat
     3421 gagtgttatt gtcgcacgtg cacttcctga tgtcagagat ggtctgaaac ccgttcatcg
     3481 ccgtatttta tacggcatga acgaattagg tgtaacaccg gataaagccc ataaaaaatc
     3541 tgccagaatc gtcggagatg tcatgggtaa ataccatcct catggagaca ttgcgatcta
     3601 tgagtcaatg gtacggatgg cacagccatt cagctaccgt tatatgctag tggatggtca
     3661 tggaaacttt ggttctgttg acggtgacgg tgccgccgct atgcgttata ccgaagcacg
     3721 gatgagcaaa atcgctacag aaatgctgcg tgatatcaat aagaacactg ttgatttcca
     3781 aagcaactat gatgatacag aaaaagaacc agttgtacta cctgctcgct tcccgaatct
     3841 tttagtgaac ggaacaacgg ggatcgccgt aggtatggcc acgaatatcc cacctcataa
     3901 cttgtctgag gtagtagcgg ctatcgattt gctgatggat aatcctgatg tgacgaccaa
     3961 tgaattgatg gaagtattac ctggaccaga ttttccaaca ggtggtttgg ttatggggaa
     4021 atcaggaatc cgaagagcgt atgaaacagg taagggttct attaccgtac gtgcaaaagt
     4081 tgagattact gagatgccaa atggtaaaga acggatcatc gtgacagaat tgccttatat
     4141 ggtcaataaa gcaaaattga tcgaacggat ttctgaactg cacagagaca agcgtatcga
     4201 agggatcaca gatttgcggg acgaatcttc ccgtgaaggt atgcggatcg tcattgacat
     4261 ccgtcgtgac gccagcgcat ccgttatctt gaataactta tataaattga catccttgca
     4321 aaattcattt ggattcaata tgttggcgat cgaaaaaggt gtgccgaaag ttttgagctt
     4381 gaaacaaatc cttgaaaact atatcgaaca tcaacgcgaa gtcatcacac gacggacggt
     4441 ctttgaaaaa gaaaaagcag aagcaagagc acacatctta gaaggtttgc gtattgcttt
     4501 ggatcatatc gatgaaatca ttgcgattat ccgcgggtct aaatcagatg acgaggcaaa
     4561 agcaacaatg atcgaacggt tcgatttatc cgatcgccag tctcaagcta ttttagatat
     4621 gcgtttacgc cgattgaccg gtttggaacg ggaaaaaatc gaaaatgaat accaagaact
     4681 cttaaaactg attgaagatt taactgacat tctggcaaga ccagaacgtg ttacagaaat
     4741 catcaagaat gaattagcag acttgaatca acgatttggt gataaacgac gcacagaatt
     4801 actagtcggt gaggtcttaa gtctggaaga tgaagattta atcgaagaag aagaaatcgt
     4861 tattacattg acaaacaatg gttacatcaa acgtttggca aataatgaat tcagagctca
     4921 acgaaggggt ggacgtggtg tccaaggaat gggtatccac aatgacgact tcgtcaaaaa
     4981 tctggtttct tgttctaccc atgatacatt gttattcttt acgaataacg gaaaagtgta
     5041 tcgggcaaaa ggatacgaaa ttccagaata cgggcgtaca gccaaaggaa tcccaatcat
     5101 caatttacta ggtatcgatt cgagtgaaag catccaggca atcattgcgg tgacgggaga
     5161 agcagaagaa ggccactatc tcttctttac gacgcgtcaa ggtacagtaa aacgtacttc
     5221 tgtcactgct tttgctaaca tccgaagtaa tggtctgatc gcaattggtt tgaaagaaga
     5281 cgatgagctc gtcaatgtgc tactgacaga tggtcaatct aatatcatta ttggaacaca
     5341 taacggctac tctgtcactt tcaaagaaga agcagtccgc gatatgggac gtacagcttc
     5401 cggggtcaga gggatacgcc tgcgtgagga agattatgtc gttggggcag ccttaatgaa
     5461 ggaaaatcaa gaagtattag tgatcacaga aaaaggttac ggaaaacgga caaaagtatc
     5521 ggaatatcca gtgaaaggcc gcggaggtaa agggatcaaa acggcgaaca tcactgaaaa
     5581 aaatggtcca ctagctggat tgaccactgt ttctggagat gaagacatca tgctgattac
     5641 agataaaggt gtcattatcc gtttcaacgt atcgactgtt tctcaaactg gacgttcaac
     5701 attaggcgta cgtttgatga agatggaagc tgatacgaaa gttgtcacga tggcagcagt
     5761 cgaaccagaa gctgcagaac aaacagttga agaacaagga tcagaagaat aaccatcaag
     5821 actaagtgtg taagctagca agaataaccg attaaaaaat ctggttattc ctgctggctt
     5881 ttttgattag ttgaatttct cttaaaatat tcgaaactgt tcattctgta ttgcttttga
     5941 ccaaaataga gtgtataatt agagcttgtg agtagtctaa ttaattagat aactctcctt
     6001 gctcttagga acctaagagc cgaaagtcca cagggaggtg aatatcaatg gaaaacacga
     6061 aatatgaaat catgtacatt attcgtccaa acattgatga agaagcaaaa actgctttaa
     6121 tcgaacgttt cgatgcaatc ttgaaagata acggagcaga agttatcgaa tcaaaagatt
     6181 gggaaaaacg ccgtttcgct tatgaaatga acggttaccg tgaaggtatc tatcacatcg
     6241 ttaaagtatc ttctccatca tcagcaaatg ctgtcaatga atttgaccgt cttgctaaaa
     6301 tcaacgacga tatcattcgt cacatgattg ttaaagaaga agcttaattg ttgtttcatg
     6361 tgaaacaata gaaaacgatg agaggagatg actattttga ttaataatgt tgtattagtt
     6421 ggacgtctga cgaaagatcc agatttgcgt tacacagcaa gtggtacggc agtcgcaaca
     6481 tttactttgg ctgtgaatcg taattttaca aaccaaagcg gcaatcgcga ggctgatttt
     6541 atcaactgtg tgatttggcg caaatcagca gaaactttag caaactatgc tcgtaaagga
     6601 acacttttag gtgttactgg acgtatccaa actcgttctt atgataatca gcaaggacaa
     6661 cgtgtatacg tgacagaagt agtagctgac aacttccaat tgctggaaag tcgttctgca
     6721 tctgaaaatc gccaacaaag cggcggattc caaagttctg gtcaatcagc tggcggattt
     6781 ggcggaaaca ataactctaa tcaaacatct caatcatcca acggtatgcc tgatttcgac
     6841 cgtgatactt ctgacccatt cggctcaagt tcaacgatcg acatttcaga cgatgattta
     6901 ccattctaaa cgaatttgat aggagggaaa tagaatggca caacaaagaa gaggcggacg
     6961 taaacgtcgt aaagtagact acattgctgc taaccatatt gaatatatcg attataaaga
     7021 tatcgaatta ctaaaaagat ttatttcaga acgcggtaaa atcttgccac gtcgtgtaac
     7081 tggtacaggc gctaaaaacc aacgtaaaat tactgttgca atcaaacgcg ctcgtatcat
     7141 gggcttgcta cctttcgtta gcgaagacta ataaatccta ggaattgaat gactgatcct
     7201 tttgatcagc tattcaattc ctttttttct gtaaaaataa cctctattcg tttagtcgct
     7261 cgaatgacga aagttatggt aaaatcaaat tagtcatata tgggaggaaa attatggaaa
     7321 ataaaaacaa tcatttatcc ccgaaacctc tcttccttat cctgtttata ttaatcgaag
     7381 tattggtagt cttaatttta ccatttcgtt ttgttagcgc ttcggtcatt ctattgatga
     7441 atttatgggg tgttcgctat tggttgaaaa tgaaacgttg gcttatttta aataaacgtg
     7501 aaaaaatcca gcaggcttcg actgtagctg aaaccaatct agcttatgtc tcagaagtca
     7561 tgcctgtagg agtgattgct tatcagccat caagccaaaa aatcgaatgg ctcaatccat
     7621 tagcattgaa aatcaaagat caaagtggat taacagagga agaattgcta gatacttttt
     7681 ttactgcaaa agaaaaaaag aaaaaccaag tctatctagc agataaaaca tttcagtttg
     7741 atatggatac agtaaagggt gttgtttatt ttaatgatat tacccaaaac cagttgttaa
     7801 aactccatca gacgaacagt cagccggtga ttggtatttt atctattgat aactatgcag
     7861 atgccattga taaattagat gacaaagaga tttcttattt gaactctttc gtcactacac
     7921 tagtttctga ctggatgaat gaataccatg tctttttcaa aagaatcaac gcagaacggt
     7981 ttttcttcgt agcacagatg atcgatctga aaaagatgat ggataaaaaa tttgatttgc
     8041 tggatcgctt tcgagcagaa gctgaaaaac agagctcgcc tatcaccctt agtatgggga
     8101 ttgcttatgg ctcaagaacc ctcgatgaaa tcggtggaga agctcagaat aatttagatg
     8161 ttgctttagt ccgtggaggc gaccaagttg tcgtgaaaga ggtaaaagaa ggagcaaaac
     8221 ctgtttatta tggggggaaa tctgctaaga atgtcaaacg cacgcgtgtt cgttctcgag
     8281 cgatgagtac cgctttgcgt cggatttttg atgaaacagg cgatgtgttt atcatggggc
     8341 acaagtttcc ggatatggat gctttaggct ctgcttttgg tgtcagttgt ctggcacgct
     8401 tcaaccagaa aaaagcttac atcgttatca atgagaacga gatcattcca gatgtagaac
     8461 ggtgtcttga agaaatccat aagcatcctg aattagaaat gcaactaatc tctccagaac
     8521 gtgccataga gctgaaaaaa gaagaggacc tcctcgtgat ggtggattac cataaacctt
     8581 ctctatcgat ctctcagggc ctatatgagc aatttgataa gattgtgatc attgatcacc
     8641 atcgtcgtgg ggaagagttt ccaagtaaac ctctgctgac ttatattgaa tcttcagctt
     8701 cttcagcttc agagctagtt tctgagctga tccaatacca aagcagcaaa acaaatcgct
     8761 tagatcgttt tgaggccaca ttattgcttg cagggatggt tgtagataca aatagtttca
     8821 atgtccgtac aactgcacga acttttgacg ttgccagcta tttaaggtct tgtggagcag
     8881 acgcatcctt agtacaatat ttattgagct cggatttagc gacatacttg gagatcagca
     8941 atttgattgc tggaagcgac tatgtgactt ctgatattgt tgtggcggca gggacagacg
     9001 aaaaagagta taatagtgtt atagcagcaa aaacagcaga tacattgctt tcgatgatca
     9061 atatcaatgc agctttcgtc attacaaagc ggacagacgg cttgattggg atcagtgcca
     9121 gaagtaccgg cacgatcaac gtccaaatca tcatggaatc cttaggtggc ggaggacact
     9181 tcacaaacgc agctactcaa ctggaaaata cgacagttga ggaaacaaaa cagaagctat
     9241 ttgctgcaat caaagaaaat atgaatcaaa tatatgataa ggagtaatcc aaatgaaagt
     9301 aattttttta caagatgtaa aaggaaaagg taaaaaaggg gacatcaaag aagttccagc
     9361 aggttatgca caaaattatc taatcaaaaa taacttggca aaagaagcaa cgaaaagcag
     9421 tatcagtcag ttagcaggac aaaagaaagc acaagaaaaa cacgaagctg aaatcttgga
     9481 agaagctaaa aagttaaaag aattcttgga aaaagaagat actgtagtcg aattgaaggc
     9541 gaaagcaggg gaagacagcc gtttgtttgg ttcgatccct tcaaaacaaa ttgctgaagg
     9601 gttaaacaaa caatacaaaa tcaaattaga taaacgaaaa atcgaattag aaaatccgat
     9661 ccgttcacta ggctacacaa acgtacctgt taagcttcat catgaggtga cggctacgat
     9721 caaagttcat gtcgtacaag aataacaaat cttgatagcg atgttcagct ggtaagtata
     9781 accagaagga aaaaagacaa accgaaattt atctgagaaa acagctgcag caatgaaagc
     9841 aaaagctgca aatcgatttc agattgattt cggtttcttt tttgggaaca gcaaaatagc
     9901 ataaagaaaa cgagtataat aaaagagaac tattttttta ttacactttc ggtacgaatg
     9961 atgacttttc tctcattttc ttgtaaaatg aagcaattga agacaaggag cgatacgtat
    10021 gaatgaagtg tggcaggatc gaatcccacc gcaaaatatt gaagctgaac aagcggtttt
    10081 aggctctgtt tttcttgacg cagaaacgat cattgatgcc atggagtaca tagagccaaa
    10141 agacttttat cgtcgtggcc atcaaattat ttttgagaca atgatcgagt taaatgatcg
    10201 taatgaagcg attgacgtgg ttactgtcaa agaccggctg gaacaagcca atttgttaga
    10261 agatgccggt ggtttaagtt atctctcaga cttggcatta gcagttccga cagcagcaaa
    10321 tatcgtctac tatgcgaaaa tcgttgaaga aaaatcattg ctgcgtacat tgatccagac
    10381 agcaacagga atcgttacga aaggattcga gcaaggagaa gaagttcaaa caattttaga
    10441 tgatgccgaa cgcagtatct tggaggtttc ggaaaaaaga aatcgaagcg gctttttatc
    10501 tattgctgaa gtactgaact ccacgattga aaatatcgat cgactagctc aaaacaatga
    10561 agaaatcaca ggtctaccaa ctggctatca aggtctggat aaaatgacag caggtttcca
    10621 gcccgaagaa ctaatcattc ttgctgcacg tccagcggta ggtaaaacag cgtttgcatt
    10681 gaatattgct caaaatgtag gaacaaaaac agatcgttct gttgcgatat ttagtttgga
    10741 aatgggggca gaatctcttg taaatcggat gctttgtgca gaaggttcga ttgaggccag
    10801 ccatttacgt acaggtcagc tttcggaaga agaatggtcg aatctgatcg ttgcgatggg
    10861 gagtctttct cgagcaaata tttttatcga tgatacacca gggatcaaaa tctctgaaat
    10921 ccgtgcgaaa tgtcggaaac tagcgcaaga aaaaggcaat ctaggcttga tattgatcga
    10981 ttatttgcag ttgattgaag gtaacggtaa agagaaccgt caacaagaag tatcagatat
    11041 ctctcgccaa ctgaaaaaac tggcgaaaga gctgaaagta cctgtcattg ctctttcaca
    11101 gttatctcgt ggagtagaac agcgacaaga caaacgacca gtgctaagtg atattcgtga
    11161 atccggatca attgaacaag atgcggacat cgtagccttc ttgtatcgag atgattatta
    11221 tgaaagagaa ggcggagatg acgatgatgg aaacgagcca aaaaatgaca acgtcatcga
    11281 agtcatcatc gagaagaacc gaagcggtgc acgaggaaca gttgaactac tgtttatcaa
    11341 agaatacaat aagttttcgt cgctttcgcc aagagaagaa ttttaaagaa gaggttgtga
    11401 gccttgagca atcccgagag ctctcgtaag aagatgcaag gagtaaccgc aggaacgact
    11461 agagtactta atttaaacag atctccaaag ggattgtgac aaaagtcttg tcacagtccc
    11521 tttttccgaa taaacggtgt tcaatcagct gacccttggt attagctcag caaacgtcaa
    11581 aatatgagga actattttga cgtttgtttc tctaatactc aggtcaaaac gctgatctta
    11641 cgacctcttt tccttaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatc
    11701 atcaaacatg agaagcagtt ttttgaaatt gctccttgtg actcagagtt aaacggcagg
    11761 ctcacaacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat acgcccaatc
    11821 aaacgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga tgcaggacag
    11881 ctttttcaca acctctgatt cacggtgttc caaaagtagc ttcttcgaaa aataagtcga
    11941 aatttcacaa aaatttgaga agcaattttc gtacattcct tcttatttct tgaagctgac
    12001 cacttctgtt acaacctctt ttttaataac cattctaaaa agataaagtt cgttttttaa
    12061 gcgtttttat aaaataaaaa taaaaatcat tcgattttat ttgaaatatg tatctattat
    12121 tgatacaatg gttcaggatt aaataatata gaacgaggtg ttcacatgtc atctgtagta
    12181 gtagtaggta cgcaatgggg cgatgaaggg aaaggaaaaa tcacagactt tctcagtgaa
    12241 aatgctgaag tgattgcacg gtaccaaggt ggcgacaatg cagggcacac aattaagttt
    12301 aacggtgtga cttataaact gcatttgatt ccttctggga ttttttataa agacaagatc
    12361 agtgtgatcg gaaacggcgt tgtagtcaat cctaaatcat tggttaaaga attagcttat
    12421 ttacatgaaa aaggtgtaga aacaagtaat ttgcgtattt ctgaccgcgc gcacgtaatc
    12481 ttgccttatc atatcaaatt ggaccaatta caagaagatg ctaagggcga aaataagatt
    12541 ggtacaacga ttaaaggaat cggacctgcc tatatggata aagcagctcg tgtaggtatc
    12601 cgtattgcag acttattaga taaagaaatt tttgaagaac gtcttcgttt gaatctagat
    12661 gacaaaaacc gtcaatttgt aaaaatgttt gattcagaac cacttgattt tgacgatatt
    12721 tttgaagaat attatgaata cggacaacaa atcaaacaat acgtcacaga tacttccgtt
    12781 atcttgaatg atgctttgga tgcaggcaaa cgcgtgttgt ttgaaggcgc acaaggtgta
    12841 atgctggata tcgaccaggg aacttatcct tttgttactt cttctaaccc agtagccggt
    12901 ggggtaacaa tcggaagcgg tgtagggcct tcaaaaatca ataaagttgt cggcgtctgc
    12961 aaagcctata cgtcacgtgt cggtgatgga ccatttccaa ctgaattatt tgatgaaaca
    13021 ggagaacaaa tccgcgaagt aggacgagaa tacggtacga caactggtcg tccgcgtcgt
    13081 gtaggctggt ttgattctgt agttatgcgc cattctaaac gagtttcagg tattaccaac
    13141 ctatctttaa actcgatcga tgtattaagt ggattggaaa cagtaaaaat ttgtacggct
    13201 tatgaattag atggcgaact aatctaccac tatccagcaa gcttgaaaga gttgaaccgc
    13261 tgcaaaccaa tctatgaaga attgccaggc tggtcagaag acattacagg atgcaaaaca
    13321 cttgcagaac tgccagaaaa cgcacgtaac tatgtacgtc gtatctcaga gcttgttggt
    13381 gtacgtatct caacattttc tgtcggacct gatagaacac aaacaaacat cttagaaagc
    13441 gtctgggcgc aaatctaagg ataaaaacga cgactgaatc aggacgttct taccagagcg
    13501 agtcgaagtg tttatctata cgataataat aacttgaaga ggtagtgaaa gagacgttta
    13561 gccttattgc cgataggctg tctcttgaac actgtttatt cagaataagg gactgtgaca
    13621 aaaaccgtgt cacagtccct ttttccgaac aaacggtgtt caaaagcttg catctacggc
    13681 actaagccca atcagatgaa aaatatggag tgctattttt atctgattgt tcttactgct
    13741 tgatgcagag cagctttttc acaacctctc cactaaacgg tgttctcctg ccaactcctc
    13801 ggtcacaagt cacaatattc aacaaacatg agaagctgtt ttttgaaatt gttccttgtg
    13861 actcggagct aaacggcagg ctcacaacct cttggtaaac ggtgttcaat cagctgaccc
    13921 tcggtattag ctcaaaaaac ggcaaaatat gaggaactat tttttccgtt tttttctcta
    13981 atactcaggt caaaacgctg atttcacaac ctctgattca cggtgttcca gaagtagctt
    14041 ctacggaaat aagccgaaat ttcacaaaaa tttgagaagc aattttcgta aattccttct
    14101 tatttcttga agctgaccac ttctgtcaca acctcttatt ttgattagaa cttgcgaaaa
    14161 taaaccggtt aaagtgtgtc tagcgcaaga gatttgctat aatagacgat aagggtaaaa
    14221 tgtaacgaga aggggttgaa aaatgtgtat caaattgtaa cagattcttg ttgtgatctg
    14281 ccataccatg tattagcaaa agaaaaagtt gattttttat caatgaatct ccaattgaat
    14341 gatcaagagt tagtagatga cctcggaaag acattcaatt atgaccgcta catgaatcag
    14401 ctgaaaaatg gtgcgatgcc tacgacatca caaatcaatg ttggccgata tttagaattt
    14461 ttccgttctt atgcggaaaa acagattcct gttttatatg tggctttttc cagtggatta
    14521 agcggctctt acagtagtgc gcttcaagcg gttgaattgc tgaaggaaga atatgaagac
    14581 cctgagatct atgtttttga tacaaaagct gcaagtattg gggaagggct tttagtattg
    14641 gaagctgtaa gattgaaaaa agaaggtaaa agcatcgaag aaaccctaga gtggctggaa
    14701 agtaattatt tgaaagtcca ttcatgggta acagtagacg atctgaaata tctagaacga
    14761 ggcggtagga tttccaaagc aagtgctact cttggcagtc tattgagtgt aaagcctatt
    14821 attacggtgg ataaagaagg aaggctgacg aatgttgaca aagttcgagg aagaaacaag
    14881 tcgatccaga caatcgtcaa tgagacggta aaaacaatcg ttgcaccact tgatcaaatc
    14941 gtttatgtgg cttacgctgg ggatctagag agcgcggaga aggcaaaacg gcttttggaa
    15001 gagcagataa agatgaaaga tgtgaagctt tatcccttag gaccaacgat cgccagtcac
    15061 acaggctatg gctgtatcgc gattttttcg atgggaatca gtcgataaaa gcagtaactc
    15121 attctgtaaa aaagcccaga aattgattgt aacaaaaaca gcgactctta aaagatagat
    15181 gagcatccta tctcttagag gtcgctgttt ttttattttt taagctaaat aggccattat
    15241 gcccataact cgttcaattg tttttgaata tctgcatttt ccaaaaattc atcatatgtc
    15301 gtatcagctc gatcgatcac acctttatcc gaaacggcaa tgatgcggtt tgccaatgtt
    15361 tggataaatt gatggtcatg tgacgcaaat aagattgagc cagtaaatgc catcaagccg
    15421 tcattcaatg ctgtgattga ttctaagtct aaatggttcg ttggatcatc taatacaagt
    15481 acgttagctt tcgaaagcat catttttgaa agcatgcaac ggactttctc tccaccagat
    15541 aaaacattca ctggtttcaa tacttcttca ccagaaaaca acattcttcc taagaagctt
    15601 cttaagaagg tgttatcatc ttcttcttta cttgcatatt gacgcaacca gtctaaaatc
    15661 gttagatcag tatcgaattc tttcgttgta tcctttggta aataagaatt acttgttgtc
    15721 acaccccaac gtactgttcc agtatctggc gtaatttcac ccatgataac tttgaatagc
    15781 gttgttgttg ttatatcatt ttctgcgacg aaagcgacct tgtcttcttt gctcaaatta
    15841 aaagatatat catctaaaat tttctttcca tcaatcgtta cacttacatt ttctacctgc
    15901 aataagtcgt ttccaatctc acgttcaggt ttgaatccaa cgaatggata acgtctagaa
    15961 gaaggttgga tatcgtctaa agtaattttt tcaagcattt ttttacgtga agttgcttgt
    16021 tttgattttg atgcattcgc actaaagcgg gcaataaatt cttggagttc tttgatttgt
    16081 tcttcttttt tggcatttgc gtttgcttga agtttagccg ctagttgact agattccaac
    16141 caaaaatcat aatttcctac ataaagtttg attttaccaa aatctagatc cgccatatgt
    16201 gtacatactt tgttcaaaaa gtgacggtca tgggatacaa cgatcacagt attttcaaaa
    16261 ttgatcaaaa attcttccag ccaattgatc gattggatat ccagaccgtt tgttggttcg
    16321 tctagcaaaa gaacatcagg tttaccaaat agtgtttgtg ctaataacac ttttaccttt
    16381 tgtccaccag ttaattcact catttgctga tggtgcaatt cttcagggat gttcagacct
    16441 tgcagtaaaa cagctgcttc tggttcagct tcccatccat taagctcagc aaattctcct
    16501 tctaattcag ctgctcgaat accatcttcg tcagtgaaat cctctttcat gtaaatcgca
    16561 tctttttctt tcagcacttc atacagacgc ttgtgtccca tgatgactgt ttcaagtaca
    16621 gtatagtctt catagtcgta atggttctgt ttcaatacgg ccatccgttc atctggtccc
    16681 atagtaacgg aacctgtgga aggctgcaga tcccctgata acactttaag gaacgttgat
    16741 ttacctgcac catttgcacc aatcaagcca tagcagtttc cgggggtaaa tttaatattc
    16801 acatcatcaa atagtttgcg gtctgaaaag tgcaaactca catcatttac agtaatcaat
    16861 ttttttcctc acttttatca ttattagttt tgacaagtct tatagaatta tataggggaa
    16921 aggtaaaaga ttcaaggcaa tcgaacattc tttgtgtttc acgtgaaaca gaatgaaaaa
    16981 aataaaaata aaagcggagc gaaaatagct actataggat ttattagttt gcaacagcat
    17041 aatttttgac gttatttata agagggagta ctataaaact gtaaaagaaa tgaaagaagg
    17101 attgaaaatg gatgaaaaaa gaaccatcga ctggggctca ttgattttgg gaattttatt
    17161 tgtacttgtc tcattgttat catttcaaga ccctgtcgga aatttagttg caattgtagt
    17221 agtctttgcg atttttgcct ttattaaagg gatatttgaa ctgtttgtac ggaacagatt
    17281 aaaagagcta acaggatata aaggaaagat gcccttaata atcgggatca tcgatattct
    17341 agttggcgtc tttttcttat tcaatattgg tgcaggagtc gcagcattgc cttttgtttt
    17401 tgcagtatgg tttattgctg attcagtttt agcgttattt actgctgact tggcaagagg
    17461 agtaagtgaa ggatattatt ggtttacgat catcgtcaat attttaggaa ttttattagg
    17521 aattttctta ttgtttaatc caatttcttc tgcgttgaca ttaagctttt tagttggatt
    17581 ctattttatg ttatttggta tcacacatat cgtctatgca tttagataag taaaatcaaa
    17641 caaataaaaa agccggcttc cgattacagt tttactgttg taattggaag ccggcttttt
    17701 tatcatggac aaatatgcac gaggtgattt ttaggtgtcg agacaaattt atgcaaaaat
    17761 gtgagaagct atatacacaa attttatctt aatactcgaa gcatagcgca gttgtcacaa
    17821 cctcttttca tataattcat tcaatactgg caaatcatgg acttcatatt tttctttgaa
    17881 tgcttggatt tcttcttcag aatattttct tggatctaaa tgcagatctt ctactggcaa
    17941 tgggcgcttg ttattgatct tcacatcgat tactacagga cgatctgatt ttgcagctgc
    18001 atcaaaggca aatcgaagtt gcgagtattc tgtgactgta aagctatttg ccccgagcgc
    18061 ttctccaact ttaccgaaat ctgcatcttc taaaaataca ccaaatttct tttgttccgt
    18121 atcttcttgt tctgcttcga tgaaaccaaa agaatcattc gaaaagacaa cattaataat
    18181 tggaagctga tatttgactt gtgtaatgat atcttgcatg accatagcaa acccgccatc
    18241 accacttaag gtaaatactt gtttttctgg gtagcttagt tgtgcggcaa ttcctcctgg
    18301 cacaccatta cccatagttg cgaaccatcc agatgtcgta tgtttttgtt taccgttcat
    18361 attcaataaa cgaatagaat ggatagtcgt gttaccgaca tctgtgacga aaatcgcatc
    18421 gtcttcagca atacgattga tttctttata gacagcctct ggacgaatcg gttcttcctc
    18481 tcgatcttca aaagatttca accaatttac ccatttttct ttattttctt gatttgctag
    18541 gtaccagcta tcagcagggc gagcatcccc taactcgaca agtctttgca aagctgtgtt
    18601 tgcatcaccg aggatcgaca aagatgtttc atggcgtcgt ccaaattttg acgcatcgat
    18661 atcgatttgg ataaattcag cagttggatt aaaaaatgca cgtccaaatg ggaagtcgct
    18721 accgacaaat aagatcagat cggcttctgc caatgcttca tttccgggtt tagtagcgac
    18781 acgtccagca aaaccaagaa agttttcata atcatcagga ataattcctt ttgctagaac
    18841 ggaagcgaca acaggaactg aaaaatgttc tgagaattct ttgatttgcg gaaatccgtt
    18901 tcgcgttcct tgaccaacat aaagaactgg tttttttgct ttttcaagat aaggcaaagc
    18961 agctagcaaa tctttttctt ctggtaatat cacacttgtt ttgtgagtgt gagcggtgga
    19021 gaaaggttgt tcgtcaattt cttcaaaacc aagatcaaca gggatagtca cgatagccac
    19081 gcctttatgc tcataggcgg ctttaatggc ttcatctact acatgcggaa gactttgtgg
    19141 tgtcatgact gtacgattgt atacgctaac atctgcgaaa ataggatttt catttagttc
    19201 ctggaaagag ttatagttca tggatgtaga agcaacttgt cctaagaacg ctactagagg
    19261 cacatggtcc atctgtgcat catacaaacc attgatcaaa tgtgtcgcac caggtcctgc
    19321 agagccgaag acagcgccga ctttccccgt taattttgca tctgcagcgg ctgcgagtgc
    19381 gccgacttct tcatgacgaa cttgaatgta gttgatttcg gttttttctt gatatagcgc
    19441 atccataatt gaattgaaag atcctccggg aatgccgtaa atatggtcga taccccagct
    19501 ttcaagaact tttaccattg cgattcctgc attgattttt gacatggtgt ttcactcctt
    19561 tagcaatgat gatacataaa ttttttgtat ctagagtata gaacttttta tcgaaaatcc
    19621 caactaattc gtcttttcac aaaaatgtag tcatgttttt gggaatatgc tagagtagta
    19681 catagataga ttcaagagag gggaatggca tggttgaatg gctattgaga cttgaacctt
    19741 ggcagcaagc acttgctggg acagctttta cttattttat gacagctctt ggtgccgggt
    19801 tggttttttt ctttaaagaa atcaaaaaag aagtgctaaa cttgatgtta ggatttgctt
    19861 ctggtgtcat gattgctgca agtttttggt ctttgttaga tccggctatt acaaaagcag
    19921 aagaaaatgg agatattgct tggctagttg taagtattgg gtttggttta ggcggtgtgt
    19981 ttttatatat ggcagataaa acactgcccc atatgcattt tggaccacaa catgaagcag
    20041 aaggattgcc tacccattta aaacggacga tcttgcttgt tttttctatt acattgcata
    20101 acattcctga aggtttagcc gtgggggtag cattcggtgc agcagcaact gcggataatc
    20161 cgacagccgc tatattagcc gctgtttcgg tggcgctagg tattggtatc caaaacttcc
    20221 cagaaggagc agctgtatca atcccgttgc gacaagaagg tctgagtcgt aaaaaggcct
    20281 ttgtttacgg acaagcttct ggaatcgttg aaccaattgc tggagtgatt ggagcgctac
    20341 tagtaacgaa agtggagctg ctgcttcctt atgcacttgc ttttgccgca ggagcaatga
    20401 tttatgtcgt ggtggaagaa ttgatccctg aagcacagca gacattgtcc agcaaacgtc
    20461 attttgcagt ttttggcgtc atgagtggat ttatcattat gatgattttg gatgtggcat
    20521 taggataaat aaaaaaaata tatatgatat atcaaatagg gccgcgaact tatatcgcga
    20581 ccctattttt ttagtaagtt atatcataat gaagtgaatg gtatcataca ttaataatag
    20641 ttttttctat gtgtatttcc tttgatccag cgaacaagcc agatgataag tgcgattggg
    20701 attagaactt tgaccgcaaa ccataaaagc ccgcttagga cactgacaaa aagactgatg
    20761 acgaagctag ctgcaattag cactaaaaga accatgaaga tatttaagaa catatttttt
    20821 tgacgcatac aaaaccctcc tcttgttcat tctttactat agaaaaagta taacagggat
    20881 tgtttaaaaa cacctccaac tgaagactga tttttacttc atctacttaa atgatcaaac
    20941 aattgctttt tctctggttt atcatctaaa atagagcgga aaaggaggag aatatcatgc
    21001 ctaaaaatcg tcttgaagca tttacagatg ctgtaatcgc gattatcatg actattttag
    21061 ttttagaact tcatgaacct aacggagaca cgtgggaggc gctcgctgca ttaggttacc
    21121 gattttttat ctatattgtc agttttatca ttttagcgat ttattggaac aatcatcatc
    21181 atttatttca agttgtacat aagataaatg gacgggtctt atgggcgaac agtttctttg
    21241 tttttgcgct ttcgcttttt ccgtttgcaa cttcatggat cagtgagtac atcaatagcc
    21301 tggctccaga aatgacttat ggttttgtta ccttgaccgc taatattgcg tattatatct
    21361 tggcaaaaga acttgtcaaa gcaaataaag gccacgagaa agcattgaag gcgatgttga
    21421 ataattacaa gaaatcgtat ctatcgattg gtttgaatgt gttaggaatt gtcttagggt
    21481 atttagtagc gccagtagcc gtgctgatcg caaacatcgt gattctagct gcttggatca
    21541 tcccaagtcg taccatcgaa tcacaatatc ggtaaaatga tccgtgttta aaatttaaga
    21601 gaaaaatata gaaaaaataa ataaacgatc aaatatccga gtgatactag tattcaaaga
    21661 gggatgcaga tggatcaaaa aagaattcat gtaggactga ttactagttt tgtcacgatg
    21721 cttatttatt tggatcaagg tcatttgact tggacagtag ttggtgtcgt aggatttttg
    21781 acattattca cagcaataaa aaaataaaca tctttatgta atctgatgtt ctatctattt
    21841 aagaaaagca tgtttacagc agtatgattc tgttgtaaac gtgtttttat ttgtggaaaa
    21901 tttaattttt agaaaagtgt atcgaaaaat agatagttag tttttccaca gtggataact
    21961 tcgtaagaga taaaaataaa aaattagtca aagaagggcg tatagatttc ttttgatagg
    22021 agatatacaa cagaaatgaa taaatattaa cgtgttttca aacagtataa aacggtgtgg
    22081 tttttggaat aaaaaaataa catatctcat tttacacttt tgttatttta tacttaaaaa
    22141 ataacgacac agaaaatacc gtgtctcaga tttctctact acttctaaaa actactgttt
    22201 ttttcctttg aaatgccttc aaaaagtagg atcaggcaga atatactggc agaatcaagg
    22261 agaagggagc ctaaataagg agaaaaacca tagaaaatat aaagtaatta cttataaaac
    22321 a
//
LOCUS       DACCXO010000040        22203 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.041, whole genome shotgun sequence.
ACCESSION   DACCXO010000040 DACCXO010000000
VERSION     DACCXO010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.041"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(88..606)
                     /locus_tag="IVY70_001384"
     CDS             complement(88..606)
                     /locus_tag="IVY70_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP7958521.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(608..1225)
                     /locus_tag="IVY70_001385"
     CDS             complement(608..1225)
                     /locus_tag="IVY70_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP7958522.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(1222..2085)
                     /locus_tag="IVY70_001386"
     CDS             complement(1222..2085)
                     /locus_tag="IVY70_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7958523.1"
                     /translation="MTANDGLFQKDQCESANKKILSYVLMTVIGIILIIPLLWMVFTS
                     LKPMEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFI
                     AYGFAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGN
                     AFNIFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWN
                     DFLGPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFI
                     EGMDLTGGSKG"
     gene            complement(2072..2983)
                     /locus_tag="IVY70_001387"
     CDS             complement(2072..2983)
                     /locus_tag="IVY70_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7958524.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(3024..4358)
                     /locus_tag="IVY70_001388"
     CDS             complement(3024..4358)
                     /locus_tag="IVY70_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7958525.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4566..5396
                     /locus_tag="IVY70_001389"
     CDS             4566..5396
                     /locus_tag="IVY70_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAP7958526.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(5640..5889)
                     /locus_tag="IVY70_001390"
                     /pseudo
     CDS             complement(5640..5889)
                     /locus_tag="IVY70_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6080..6274)
                     /locus_tag="IVY70_001391"
     CDS             complement(6080..6274)
                     /locus_tag="IVY70_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958527.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(6779..6964)
                     /locus_tag="IVY70_001392"
     CDS             complement(6779..6964)
                     /locus_tag="IVY70_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958528.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(7814..8155)
                     /locus_tag="IVY70_001393"
     CDS             complement(7814..8155)
                     /locus_tag="IVY70_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7958529.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(8155..8385)
                     /locus_tag="IVY70_001394"
     CDS             complement(8155..8385)
                     /locus_tag="IVY70_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7958530.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            8672..9010
                     /locus_tag="IVY70_001395"
     CDS             8672..9010
                     /locus_tag="IVY70_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958531.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(9215..9454)
                     /locus_tag="IVY70_001396"
     CDS             complement(9215..9454)
                     /locus_tag="IVY70_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAP7958532.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(9430..9777)
                     /locus_tag="IVY70_001397"
     CDS             complement(9430..9777)
                     /locus_tag="IVY70_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP7958533.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(10509..11090)
                     /locus_tag="IVY70_001398"
     CDS             complement(10509..11090)
                     /locus_tag="IVY70_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7958534.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(11273..11899)
                     /locus_tag="IVY70_001399"
     CDS             complement(11273..11899)
                     /locus_tag="IVY70_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7958535.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(11921..13252)
                     /locus_tag="IVY70_001400"
     CDS             complement(11921..13252)
                     /locus_tag="IVY70_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP7958536.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(13407..13808)
                     /locus_tag="IVY70_001401"
     CDS             complement(13407..13808)
                     /locus_tag="IVY70_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP7958537.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(13894..14100)
                     /locus_tag="IVY70_001402"
     CDS             complement(13894..14100)
                     /locus_tag="IVY70_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958538.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(14195..15697)
                     /locus_tag="IVY70_001403"
     CDS             complement(14195..15697)
                     /locus_tag="IVY70_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP7958539.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(15715..16170)
                     /gene="argR"
                     /locus_tag="IVY70_001404"
     CDS             complement(15715..16170)
                     /gene="argR"
                     /locus_tag="IVY70_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP7958540.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(16268..16885)
                     /locus_tag="IVY70_001405"
     CDS             complement(16268..16885)
                     /locus_tag="IVY70_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958541.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            17106..18479
                     /locus_tag="IVY70_001406"
     CDS             17106..18479
                     /locus_tag="IVY70_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7958542.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            18554..19666
                     /locus_tag="IVY70_001407"
     CDS             18554..19666
                     /locus_tag="IVY70_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP7958543.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(19738..21375)
                     /locus_tag="IVY70_001408"
     CDS             complement(19738..21375)
                     /locus_tag="IVY70_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP7958544.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(21543..22151)
                     /locus_tag="IVY70_001409"
     CDS             complement(21543..22151)
                     /locus_tag="IVY70_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7958545.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         6467 a         4530 c         3871 g         7335 t
ORIGIN      
        1 atgaataaac aactacgctt tcctcgaaac aaaaaaataa agcacaaatg cgcatttacg
       61 cactcatgct ttattcgtac aattaattca gataacggca gcgcatagga tgccaataac
      121 acttcctaaa atagctcctc ctaaaatgtc agtaggataa tgtaaaagta aaaaaatcct
      181 cgaataagcg atcaaacaag caaataaaaa ggctaatatt cctgcaactg gacttaattc
      241 gaacaacgca gctactgata aaaaggctag cgctgtatgt cctgaaggaa aagaagaagt
      301 cttcggttta cttgtgtaaa ggacaagccg acgatttttt tcataaggtc ttttccgtct
      361 tgtcgttttt ttgataaaaa gcaaaccaac aaaaaaatag atcaaactgc cgataagaat
      421 ggctagacca tattctctag tatcaggtat caagactaaa acagcagcaa ggagcaacca
      481 aataaaacca ccattagaga tattactgat aaatttcaaa aaattgattt ttcgactatc
      541 cgttttccgt tgcttggcga acttgttgat aatactatca tcgattttag caatcaaacg
      601 caacatgtta ttcctccttt ttaagaacct tttcttgcgt acgatggata aaaagcagga
      661 cttgaaaata aaacaagaaa ctgaaaccta ataaagcaaa atagattggt cctgcaaacc
      721 agaatgcgac aagaaggatc aacgtgcctg ccaaatagaa aagcgcgctt ttgaaatcat
      781 taaaaaacaa gaaaaatgca gtaatccaag aagcttcttt ttgttccagt tgaagcaaga
      841 ttttgtaact aaagagaagc gcgcttaata gaagcaaaaa caacgtagta tacatcaatg
      901 tatagccaat tcccccaccc caatagcgga aaatctgata atcgatcaat agaagaagaa
      961 aataaacact tgctaaacta tcttgctttc cataaagaga gaagttctca cgaaactttt
     1021 tccagaatag tgaaccgagc tttttctctt catttttttt gtaatgataa atcatataca
     1081 gaactgttcg acttgcagtt cctaatgtga aaacaggcaa gacgcacagg aaccatagta
     1141 gctgtaaatg gataagacgt ggaatccatt caatgatttc taagaaataa tcattggctt
     1201 ttttaccaat cgatcgattc atcatccttt tgaacctcct gtcaaatcca tcccttcaat
     1261 gaaataacgt tgtgcaaaaa agaacagcaa aatcgtcggg atcaagacaa gtgttgctcc
     1321 tgccatcaga taattccatt gtgtcgttgc ttggttctga aaaacttgca agccaatctg
     1381 taacgtgtat ttttcttgat cttggatata taacagagga cctaagaaat cattccaagc
     1441 tgcgttgaag gaattgatcg caatggtaat caaggcaggt ttcgtcaatg gaagcatcaa
     1501 atgactccaa atatacaaat gattcgctcc gtcgatttca gcagcttcga tcaattcatt
     1561 attgatcgat aagtaaaatt gacgcatcag aaatatattg aacgcattgc cgaagaacga
     1621 tggaacgatc aggggcaaat aagtacctac ccagccgatc ttggagaata atacatattg
     1681 agggatcatc gttacaaacc ccgggatcat catcgtagaa agaaccaaag caaacatcag
     1741 ctttttccca ggaaaatcaa gcttcgcaaa tccataagca ataaaagaat ttgaaaggac
     1801 attaccgatc actactagca cagtgatgaa taatgtattt cttgcatagc gccaaaaagg
     1861 aaaggcggca atcgtatcca gatagttttc ccatacgatt ttttctggga aaaaggtcgg
     1921 aggataacga acgatttctt ccattggttt caatgaagta aacaccatcc aaagcaatgg
     1981 aatgatcaaa atgattccta ttactgtcat gaggacataa cttagtattt ttttgtttgc
     2041 cgattcacac tggtcttttt gaaataatcc atcatttgcc gtcacctcca gaataataga
     2101 cccacttagg tgcaagcttc aaattgacaa acgtcagaac caacacgatc agaaacagga
     2161 tcattgacat agccatcgca taacccatat caaaagctac gaacgccttg ttccacatat
     2221 gcagattata aaaaagcaaa gaatttgcag gtccgcctgt tccatttttc gtcatgacat
     2281 aagcttcttg aaaaatttgg aaagaaccga tcgtagaggt gatcacatcg taaaaaataa
     2341 taggagtgat taatggcaaa gtaatacttt tgaactttcc ccaaacgcct gctccatcga
     2401 tttctgctga ttcatataaa gatctaggaa cattttgtaa tgtagctaag taaagtagca
     2461 ttgctcctcc agaactccat attttcatca aaatcaacgc aggtttcgtc cattgtggat
     2521 caaacagcca agctggtcca gggatatgga accagcccag aaatgtgttg atcaatcctg
     2581 cagatggaga aagcagctgc atccataaga tgtagactgc tactcctgat aagactgccg
     2641 gtaaatagaa agctgtacgg aatacactga ttcctttgat tttttgattg agtactgtgg
     2701 ctaaaaacac actgaaaatc gtcgtcagcg gtacattgaa gatgacgaaa taaacggtgt
     2761 tgtataacga tttccaaaat aaatcatcgt gaacgaacat tcttttgaaa tttgctagac
     2821 caatgaaatt catcttggat gtcaaattat aatcggtgaa actaccaata actgaaaaaa
     2881 gcataggccc tagcataaat accaagaacc cgataataaa aggcgagata aataaatagg
     2941 cccatcttgc ttcttttttc tttcggatag ataaacgctt caaattttct tgcctcctct
     3001 gtacctagac agtcaaaaat ggattattga ttttgcttga ctaagttttc tactgctttt
     3061 tgtgcatctg ccaatccttc ttttggtgat tttgttccta aaagaatctg gtcaatctgt
     3121 tcattcacta atgtcgtgta atccggagca tctaaaggaa ctggtgtgat caccgtattt
     3181 ttaaagtttt cgtccgccat ttgatagatc atttttcctt tatccgttag gttgtcatta
     3241 ttcactaaat tctcattggc tgtcttgctc gccatgatat caaaactttt ttcgccgaat
     3301 ttttcttgta cttctggaga agaaagatat ttgatgaatt cataagaggc ctcaggatct
     3361 tttgctccgt atggtacttc caagacgaat ccgccacccc acgaccaatg gcctgaacct
     3421 tcttcttttt ctgggagctg catcacgcca aaatcaaaat catccggtgc attttccatc
     3481 aaactagagt aataattgat attttgggca cgcatggcta ctaatccgga aataaatgga
     3541 tcagatacac cagacccaaa ttcggcttct aatttattga tcgtattttt cccataataa
     3601 tcttgccagt cttggatcca ctccaaggct tccactttat ttttagtatc aatcttgact
     3661 ttttcatctt tgtcaaacca actagtcccg ttatctgcgt ttagcgccca tacatcagcc
     3721 cctaaattcc ataatggata aaaaccgatg cgtgtaaatt cttttccttc ttttttatct
     3781 agtttacgtg ctgtttcttc taattcatcc caagttgtcg gtagatcttg ttcactgatt
     3841 ccagcttctt taaatatctt tttattataa aaaatgactt gtgtatctgt attgaatggg
     3901 atcgcataag gatcaccttc atattctact gtatcccaaa gctgcggata aaaatcctta
     3961 gaagtgtctt tttctacata tttggagaga ttagttgctt gctgtgcttc tgctctttgg
     4021 gcaacggaat ttatgtcctg tacaataaca tcgggtgcat ttccagctgt tattgcagat
     4081 aaagatttag tccagatgtc accccatggc tgatacttat aatttacatg gatctcgttt
     4141 tgtgattgat tgaaatcctc aatgatttct tcgatagctt cttttcgtgc accggatccc
     4201 caaaaggacc agaaatcaat attttttctt ccatcacttg ttttttcaga agaatttgct
     4261 gcacttgaac tggcatcgct tgcattactt gatccgcatc ctcctaataa aaacaatgct
     4321 gaacttgcca gcaaccctgt aaacaataat tttttcatat tttacctcca aattttaatg
     4381 taaccgatgc cggttttaat ataacgatat cattttgttc tgaaaataac aatttataag
     4441 gtatgattct aaaaaaatta ttaatgatac caatacttta tataattcgc gagcgctttc
     4501 tttgaatttt ttaatctttc gtagcagaat gagaatgtat taaaaataga ttaggaggaa
     4561 ttttgatgtt tagacaccag aaagaattgc agtttgaagc aaaaccagat cgtccgaatc
     4621 cattaatcgc gaaatatctg caggagctga tcggcggcca atatggcgag atgagtgtag
     4681 ctatgcagta cctgttccaa ggctggagct gccgaggaga agaaaaatat aaagatatgc
     4741 tgatggacat tgcaacagaa gaaatcggcc acgtggaaat gttggcgaca atggttgctc
     4801 gtttattaga gggtgcgcct gttgaagatc aagaagcagc gatgcaaaaa gatccaacaa
     4861 tcggtgccat tatctctgga atgaatccac agcacgcgat cgtagctggc ttaggacctc
     4921 gtcctgctga cagcgtgggc aatccatgga gcggtgccta tatcattgcg agtggcaact
     4981 tgttagcaga ctttagagct aatttaaatg ccgaatcaca aggaagactt caagtagcac
     5041 gtatttacga aatgatcgat gatcgtggcg taaaagatat gctgtctgtt ctccttgcaa
     5101 gagatagcta tcatcaaaat ctatgggaat gtgcagtgaa agaattagaa gaaaaagaag
     5161 gttcaatctt agtcccaagt actttcccgc gtgacaaaga acgaaaagac attgcttatg
     5221 acttctataa cttctcagag ggagaccaaa gtagtgaagg ttcttgggct agtggcaaag
     5281 cactggatgg cgaaggcgaa tatcaatggt tgaaagaacc taagatcgaa ggcgctgcac
     5341 caaaattaga accagtaaca ccaaaaatgt atgggacacc tccattcaag aaataaatat
     5401 ctacccgtag aaacacccgt tttttgttcc acacgctttg tgagcaaaac tgactaaagt
     5461 cattaatcat aaccaccctt caaaagggtg gttttctcta gagctataag ctcaaatcac
     5521 cggccagcgc ctaaagacgc tggctttcat attttatgtt caagccatat tgcctttgcc
     5581 agttttgccc acctcttaaa aggtttatgt actactcctt agcccttaaa aggatcgtct
     5641 tactctcgta cactcagctt atctaacgca ctatcatgtt tttcttgttc ttgaatgtac
     5701 tttttgatgg tcgcttcatt ttgtcctact tttgtcacat agtatccttc cacccaaaag
     5761 tgtcgatacc caaatttata ttttagattt gcacaatctc ttgatgatct cctgaatatt
     5821 ggctcgatat tgattataaa ttatttttct tctatacttt ggtgtgaata caatgtggta
     5881 tttacacatc tactttgtac gggctaaact atttcccttt attgctccac ccgcatagca
     5941 agtggttttg tatttcaatc gctcggcgcg ctcaattagg tctaaagaca ataaatctaa
     6001 aaacccgctg cttcactgac agaaacaacg ggttttcaga aaaaaatgaa atgaaaaaga
     6061 tctcttacgg caatctattt tatttttttg attttttata aatggaaaat gcttcaagaa
     6121 gtccaagaaa taaagccgta taaaaacaag aacctatgaa atctttattt attagatatt
     6181 caacagaaat cccgataaca ctagccaaca atatatttat cagaagtgta aaataaggat
     6241 gctttttata gaatagaact agtgttgttt tcatagactc tcctttcttc atatttccaa
     6301 ttaatcacaa attcacggca ttactagaaa aattaaaggg gcgcagaagt ggaaaaatac
     6361 tcttcgactt tccctacaaa ccaggccatc ttgtgtttaa taactatacc aaatctaagg
     6421 tacctctaaa tctaaattcc atttgtggcg gtgacctcca atttttaact cttctatctc
     6481 ccatccagta tacataacct tgatacttcg accacttata tatcgaacac acaaatacct
     6541 atccattgca ggagtactac ctaacttagt tgctacttgc atctaaataa cagctgaaaa
     6601 gataggactt actcttagta aagtatctga actattaatt tttaaactat catttttatc
     6661 ctcaactaca tgcacatctg ttgcaacttc actttctgtc tcatccagat aaatgattaa
     6721 ggaatcggga attgcaatat cttcagctga cgctggaata gttgagctaa attagtcatc
     6781 atatctttat tataaaataa ccaacagcaa aaaatacaat gaaaaacaaa cttggaacta
     6841 atgtatcatt aataaatgtt gaaaaattca gcaactcatt actgtatgat tttagcagcc
     6901 caaccaacat ataccctata aaagctaata agatggttag gaatccttta gaatttttac
     6961 tcattcatag acacttcctt aaataacttt tattatagaa ggtattgtaa ataataatat
     7021 tgacaaaaag tgactcaaaa taaaaaaaac cttacattat actggaatga actgtattga
     7081 tagtgtacct aaaagctcta ctgacataag atcgtgtaat cggtaaagta attttattaa
     7141 ttatacggga tgtaatcaat tagagaataa actaatcata aaaggtactt ttacattcat
     7201 aattgtcgct ttgagattct actttggttc actatctgac ccaattgcac attttcgatc
     7261 attacaagta ttggtgtcgt tagaacaact aaataaaaac tagcggtaga tttttcagaa
     7321 tctatgctaa tttctgtatg gatgttccag tgatggcaaa tttctctttt cattgttttt
     7381 cttgtcgcat gatatgcctt tttgtgttat tcagttaaac tatagaaatt ctttcctaga
     7441 cagatcttga tcccaaattt tacgtttttg agcatcgata tagtttaaaa tgacattact
     7501 catatctaaa gagtttagat tttttaggac taaaactgtc agttgttctt tttcttcttt
     7561 ctgttaaagt attgtcgcga cttttcgtat acgttaagat atgtcgtgaa aaaacaagtt
     7621 ttgtgacaca aaaaaacgaa tgacttgagg aaatcacctc aatcattcgt ttaaaatgct
     7681 atatattctt tagtttaaga tacggaaatt aatgccatcg accacactag tagaatgaac
     7741 gacatttact tggccttttt ttaatttcgt tatgtttagc atataaaaat attatagagt
     7801 ttacttacaa tgcttaaaac atatactgta taatctgatc aattttagcc atgtcttgca
     7861 atggtaagtt ttctattttt ttgagttttc gttccttaaa atcaagagat tttaattgtg
     7921 taataattac ttgtcctttt atatctagct cttcaggtaa tgagtaacgt gtcggtaaat
     7981 tctttatagt ggaagtaatt ggacatatga ccgaaaacat tgttttacgg ttaaattcat
     8041 aacgagagac gactagtcct ggtcgtcttt tttgaatttc ttttcctacg gagggatcaa
     8101 aatcaatcca tacaatatca ccttttttag gaatataaat tctttcttct gacactataa
     8161 aatttctctc ccttcaggag ttaaatcttc ccattcgtct ttctcataat actcggcctc
     8221 ttccccacca ctaaatggat cttcaatttt tggaactaac gtaattgttc catcatcaga
     8281 atacacaacg atatattctt ggttttctga aggtttcttt ccgttatcag aaggtaaagt
     8341 taccactaca gaacttcctt gtaatctaga ttttgtaaca agcattatca tcactccctt
     8401 aataaaagta tactatataa cactaagtaa cgcaaaattc ccctttgtaa actatctaga
     8461 aaattgtttt gtttaaagta tatcttattg gatataaata gaaataaatt gtagccagtt
     8521 cattttttct ttaactaaaa aaataatcca agaaacattg aacctatgga tccaaaagct
     8581 acccaaatac tatagctaac cccccgctac cttcaatttt gcagataaaa cgctttggga
     8641 gaaaatttca aagacaacag gccatcctat catgcccgat tataaacaaa aagctggcta
     8701 ttccttttgc tctgacaaat ttcaattggg aaagttcacg aaactagcag gtgaagaagt
     8761 ttcaactgtt cggatcaaag aatgcccgct tcaaatagaa acattggttc aacgaatttc
     8821 aactagacaa gactttgcta ttgtagaacg tgagatcgta gctatttttg tagaagaagg
     8881 gattttacat gatccaactc atatcaatgt ggataaatgg caacctctta tctataaatt
     8941 cagagaatat acaactgcca accagtcgtt aactttgaat tttagattcc aagaattcaa
     9001 agagtcataa tgataaacat cgttaggtat aaataaaaaa gactcgcagc acgagtaaaa
     9061 aacaaataga agctatacat tagaaaggtc gactgtgacc ctatagatgc agtatatgca
     9121 gcttatgagc atttcttctg ttctctttgt ttttgggatc tgtgctgaag tctttttcgt
     9181 tttttgccgc tctttctttg aaaaatcgat tatgttatac cttctccatt actaattcag
     9241 gcatctgctg agtaatgatt tttcgcgcaa aagaaatatc tgttacccaa ggttcgattt
     9301 gttcttcagt caagacaagc ggcattcggt catggattgg tgcgactgtg cggttaggtt
     9361 cagtagtcat tatgattgat tctgccattt tttcatcctg atggctacga taaaaaccgc
     9421 caatatacac tattttttcc ttatttgaaa acataaattt gtctttttct tcattccact
     9481 cattgggaaa acacaacgat tttgcaaaaa tggttgatga aaggtctttt tttcttctac
     9541 tgtctctgca cgggcattaa tcatcaattg tcctttttta aatccggtaa atccccactt
     9601 catgatttcc ggagtgagga tgttttcttc ctcttttctt aacgtcacaa caagatttga
     9661 cggaaaaact tctccagctg ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc
     9721 tcgataataa ttctgaagga gctctgactc tagatcaaaa aaataacgac cacacatcct
     9781 aatccctccc aatctttttt tctatcatac actgcaacaa gaagttcgca ccaacaaaac
     9841 gctttcaatt tttgtagaac taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg
     9901 tattctcctg ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgct
     9961 ttttgaaatt gttccttgtg actcggagtg aaacggcagg cttacgacct ctgggtaaac
    10021 ggtgttcaaa agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagaaagcta
    10081 ttttcacaaa atttttctta atactcgaaa ctaagcgact ttttcacaac ctcttcctta
    10141 aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagca
    10201 gctattttta tttggctgtt cttattactt gatgcagggc agctttttca caacctctcc
    10261 actaaacggt gttcaatcag ctaaccttcg gtattagctc aaaaaacggc aaaatatgag
    10321 gaactatttt gccgtttgtt tctctaatac tcaggtcaaa gcgctgattt cacaacctct
    10381 aatacacggt gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaattt
    10441 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
    10501 tctaattatt aaagaacctt ttgcgaagtt aacaatcttt ctttgatcaa ttgaaaattc
    10561 tgcctaagta gtgatagatc ctgtgtgact ttcaacaata agatacttcc ctggatttca
    10621 gcaatcaacg ctttggcttg acggtctgct tcttcatcgg acagtttttc ttcagaaagt
    10681 gccaggatat tttgtttcgt atacttgacc cattgattga ctaattctag tagcaacaga
    10741 cggtattctt catccactgt actcaactca ataatcaaat tacctacagg acagccatga
    10801 taagtgatct gtgtctcatg atcagcgaaa atccagtcca acatttcttc taatgcttct
    10861 tctggctgtt ttccagcatt taagatgtct tcgaacaatt ctgtctgcca tacgtccata
    10921 tgcgcatgga tcaccgctag acaaacttct ttttttgaag aaaagtaata ataaaattga
    10981 ccttttcctg tatcagaagc tgctagaata tctcggattg acgtttcatg atagcctttt
    11041 ttataaataa gatctttggt cgcttccaag atcttttcct ttgttttcaa tgatattctc
    11101 ctttttcatt tctcatagtc ctatattata cagtaaattc atcatttttt cttcttaatc
    11161 gtttaaaaaa gactgtgcca aattatctga cacagccctc actataaaac tgttctcata
    11221 tagctagatg ttcattgatt caagaactgc gaggacaaaa gcaattccta tcttatgata
    11281 gttgctctaa tacttcctct gttgtcatca cttgatctgc aataaagcca aagttcgtca
    11341 acgctgcatc cattgcttct tggccaggag caccaattgc atctgtcgct accacaacat
    11401 tgaatccact ctctaccaat tcacgcatat gggaatcgct acacaagttt ccgttcattc
    11461 caccaagaat tactgtgtcg attccatttt ttctcaattg gagcgccaaa tcattgcttt
    11521 ctggtccaaa gattttatga ggagagctga tgatcgtatt ttcatccaaa taaggtttca
    11581 attcttcgat aaaatcggct cctgaacctt cttccactga ttcatattga ctttttctag
    11641 caaacatctg ctgctcaagc atcatatgtt ctcctgcacc gccaaattgc catttatggt
    11701 catgtgggta aaaataatga ggagaaataa agagtttgta tcctttttct tttgcttgtt
    11761 ttaccatttc aatcatattt tcgatcgtat tgtatttttc taagatatct tttgtcaaac
    11821 caaagccacc gcctgctggt ttcaagaact cgacttgcgg gtcagtgaga actactgctg
    11881 tatttttatt attgaacatg gtggattctc cattcgttat ttattttaat acatcattta
    11941 gagcttcact cattgtcgga tgagaataga tttgatctct caataacgta taaggaagtt
    12001 ttccattcat agctagcgaa atcaagttga tgacttcatg agaatcttcc gcatagatac
    12061 ttgcccctag aatcaagtca gtttcagggt cgactaaaac tttgaataca cctttaggat
    12121 cttctaatac ttgtgcttta gggacaccgc ttgtcatata cttgaataat ttgtaattga
    12181 cgccttgtgc tttggcttgt ttttctgtta aacctacatt ggataaagtc ggagtgataa
    12241 atacactgta aggcacagct ttccggttat tcgttgttcg tttattctcg cccttcaact
    12301 gatccataat gattcgataa tcatctagag agatataagt gaactgcaat ccgcctttga
    12361 cgtctccaat cgcccaaatg ttgtctgcat ctgtacgaag gtattcatcc acagcgattg
    12421 ctccgcgatc tgttagttta acatctgtat tttctaatcc aagtgatgct gtacttggtt
    12481 ttcttccagt tgcagccaaa acttttgacg cttggattgt tacttcttgg ccatcttctg
    12541 taaaggtgac ggcagctgtt gattcttggt ctgctacttg atctacagat acacctagat
    12601 ggaattcgat tcccgcattt gtcatgtctt ctaaaatcat ttgcgcgata tcttcatctt
    12661 cacgtggaag aaattcagaa tgggcatcaa gaacgacaac tttagatccg tattcattga
    12721 acattgatgc aaattccaag ccaatatatc cagccccgat gatcactaat ttttccggta
    12781 attgttcctg atccatcgct tgcgtagaat caatcaagta gcgacttgta ttcaatcctt
    12841 tgattggtaa aatgattggt tcagaaccag tattgatgaa aatccgttcg ccttcaacaa
    12901 tcactttttc accattattc gtcacttcga tttgatggtt agaaagaaaa cgtgctgtac
    12961 cgtccatcac tgtcccagtt gcctcatcgc tgatcatatg ataatttttg gcgcgaagca
    13021 tgcccgtcaa tttttctttc cgagcaacag cctctgtaaa gtcgattcct ctttctccat
    13081 tgaaaatcaa tgatttagaa ggaatacagc cgatgttgat acatgttccg ccatacatac
    13141 gtgttgactt ttcaacgact aaaaccgatt caccttttga agctaacgcc ttcgctaaag
    13201 tcttaccgcc ctttccaaaa ccgatcacaa tattttgata tttttccatc atttttccct
    13261 cgcttctttt ttacctaccg attggtacaa aaatatgata accgataatt gtctattctc
    13321 caaatcatct gctttatttg tcaaaaaaaa ccagacaaaa agcggtcctt tcacgttttt
    13381 agtctggttc gatttccttt attcttctaa acaatcgtta cttctacatc gatatttcct
    13441 ctagttgctt tggagtaagg gcagaattca tgcgcatctt ctgcaatttt ttgggcttct
    13501 tcttcgctga tgccatcgat atgcacttct aatactacac cgatctgata gccgccatca
    13561 tctaaattat aaagagagac acgtgctttt actgtggact ctgctgtcat tcctgcttct
    13621 ttgatcatat gttgtaccgc cccattgaaa caagaagcat aacctgctgc aaataattgt
    13681 tctggatttg ttccttctgc atggattcct ggaggagtga ttttcatatg catcgagcca
    13741 tttggtgcct cgacttctcc attgcgcccg ccatggttga tcattgctgt ttcgtatgct
    13801 tttttcattg tactgctcct ttcgattgac catttgctac ttggctataa ttatagtgta
    13861 tgacaagtaa ggcgctttct acaaaaattc tgcttattcg aataccagaa tgccgtcttc
    13921 tactattttt ccaaaaaaca aaaaaatact actcgtgaac aacagcgtca tatttgttcc
    13981 aaaataaata gcaatctttt ctacataatc ttttatatga ttgattttaa gatacatata
    14041 taccagaaag ttgaccccat aaaccaatac tgttaaaaaa ataaccatat tcgttaccat
    14101 tttttcctcc ttttgctggt ctccctacca gcactcgatt taaccatacc actttttttg
    14161 ccgtccaagc aactagcctt tcaaaaagac tggtttattc tgctgttaat gattcaagat
    14221 cagaaaaatg tccgaataat tttgctaatt ttactaacgc caacttgttc tttccgaatt
    14281 catcattttc tatctgtaat actagtaacg gctcatgtaa gcttaaacgg atcaaaaacc
    14341 agccgtttcc gtatggctct tctagatcat atcgaatacc ttcttcattt tccgggtcga
    14401 agttcatccc tttttcttct tccaacagtc gcttcatttc ttctaatatc ttgtttcctt
    14461 gttcgcgtgg ttcatcgctg aacaatttta atcggatttc ttgtacttct acgggctgtt
    14521 tcaactcttt gatttgttca tctaatgtga taccttttct ttttaattct ggcaaaagca
    14581 tcaggatctt tgcgatgaca tatgcaccat catccagaaa atagttttcc ttgaatgcag
    14641 cgtgtccgct tgtttcgatg gctagctgtg tatctttccc aagacggtta agttctaatg
    14701 ctttattgat tacatttcga tagccggaaa tataacgaac ttggatgcct cctttttctt
    14761 caatgaatct ttgtagatga gaagaagtag gggaattcgt cacgattctt gttcctggat
    14821 gctcttttaa tacgattgta cttaatactg cgatcaaatt atttcgattg atcactgttc
    14881 cagaagcact gacaactgca gaacgatcca catctgtatc gaaaatgatc ccaagatctg
    14941 cttcatttgc taaaacagcc tgcttgatac tttccatcgc ttctttatta tcaggatttg
    15001 ggatatgatt aggaaatcgg ccatctggtt ccaaaaattg ggaacctgtt gaatccgcac
    15061 ctaagctatt caatactttt tcagcaaaga atccaccagc accatttccg gcatcaacaa
    15121 tgattttcca accatttagt ggtttgcttt ctagtgttcc aatccccttt ttaatttttg
    15181 tgactaaatc ttgcgcataa ggtgtcaaaa ggtctgcttt ttctacgctt ccagtttgtt
    15241 cgctttttat tttttgctgc tgtgatagga taaaagcaat atcttctttt tctgctcctc
    15301 cttctgaggt aaagaatttt attccattat aataataggg aagatggcta gcagtcagca
    15361 taaccgcagc atcgcacgag aactgttcaa attgagtgga cataaacata gcaggcgtag
    15421 tagctaattc gaaatcgatg acatgtatcc cttgttcttt cagactttgg atcaacgcct
    15481 ctttcagtgc tggtccgctt atcctactgt catgaccaat cccaatcgtc cattttttat
    15541 ttggatcggt tgatttcttc tttttcttta tccagtttgc tagtccccag ccgatctttt
    15601 ttaccatgtc tgtcgtcaag tttacttcat atccttcaac tggtaatgcg attcctcgaa
    15661 tatccgatcc attttgcagc gctttcatat ctttcatata aaagtcctcc tcatttattc
    15721 cctcattttt tccagctttt ctgcaaatcg ctgtgcttct tcttctgagc ggcaaataat
    15781 aatgaatgta tctacaccgg ccatcgttcc gacaacttca gggtaagcca cttcatctag
    15841 ccaattcgtt accacatcgg caccatttcg ttcagtcaat acgataacca tgaactgaac
    15901 agactggata cgcaaaacct cgcgtctaat tgctgtacgc aaacgttctt catttaaaga
    15961 aacagatggc tggttaaata atgtatagcg tactattttg ttttcatttt gactttttac
    16021 taacttcatt tctcgaatgt ctcgagaaat cgttgcttga gtcacatcgg cgcctgcttc
    16081 atgcagccgc gcaagcagct cttcctgtgt cccaataggt tgttctgtaa tgatctggcg
    16141 gatcagtgct tgtcgctgtt tctttttcat attagacacc tctcaatgtt gattatagca
    16201 aaagataagt aatatttctt catattttgg tattttttac ttttattttt tgttttcatt
    16261 caggttgcta acctttttct attttaacac gcaaaatcgt cttcattttt tcaggtgctg
    16321 attttcttgg atcgctgatg taaatctctt tgtgttcttt agaagctctt tcatagcctt
    16381 cttttgcgat gaatgcttct aatttctcaa acgttgctgg ttcttcggca tagggcccga
    16441 cgtgtaagat ttgggcacaa agaccttctt ccatcttttt gaatactact tgttctttca
    16501 agtcttccgg aagtttgcta gaacgctgaa tggcttcttg tgctatcgct tctgtaacaa
    16561 agtcaggctg tcggatcatc aattgataag aaaaatgttc ttttttcatc actggttcat
    16621 gcaaatactt ttcttgcagt ccccattgtc cttccagtgg atagacagta tacatctgat
    16681 aatcaggaat cagccaattt tttttaggcg acatgcgtat tgcatagctg actgcatata
    16741 gacaggctac tcttcgttta aagtcttcct gattcgggtc gccgatccct ctgatgctaa
    16801 agaatgattt tttaggaacc tctagtattt gcgggctttt tttcatacta tagctcttgt
    16861 ctttacgcca ttcaattttt tccatttttc ttcctccgcg aaattccatt tagttattta
    16921 ttatcatacc tctttttgag tgacttggct aatcgtgttg acactatttt tcagtttgtg
    16981 ctagaataag tgaagtaaat aaatcgtaca gataacacgt ttcggcgtgt cgagagagag
    17041 gcggtttcta tttttataat agaaatcact ctcttttttt acgaaatttt ttagaggtga
    17101 tgtaaatgac aaaacaaaaa cagctgcttt tagtacctgc agtaattgca acagggatga
    17161 tgtcttttgc gggcgtattg atcgagacgg ccatgaatgt aactttccca actcttatca
    17221 gacagtttga tttaacgact gcacaagtcc aatgggtaac aacgatttat cttttgatga
    17281 tttcgatcat tgttccgctg tctacttatt tgaatcgcaa ttttagtctc aaaaaactct
    17341 ttttagcttc aaatctgctt ttcttggcag gtataatgat cgatttcttc tctcctacct
    17401 tcatggtcct attgtttggt cgattgctcc aaggtgcagc gacaggtatc gctcttccgt
    17461 tgatgttcca tattatcttg acatttgccc ccctcaatcg acgaggcgcg atgatgggtg
    17521 tcggtacttt gacaacggcc attgctccag caattggccc cacttatgga ggcattatga
    17581 cttcacattt tacatggaat catatcttcc tgtttcttgt gcccgttttg tttctttctt
    17641 tgattatcgg ctggtttgct attcccgaga tggcagtaca aaaaagtggt tatttggatt
    17701 ggataagcgt tttaggaatt tgtcttttgt ttagtggaag tctaacattt ttgagtacgt
    17761 tgggaacagt ttccagctgg attgctttaa tcatcggcgg tattggctgg ttcctttttt
    17821 actatcgaag taaaaaagtg gagcatccac tgatccgact gtctattctt tcaaataaaa
    17881 cattccgctt gtttttgatt ggcttcttag tttatcagtt tttattactt ggtgtatctt
    17941 ttgtcttgcc gaatttcctt cagatcgttc aaggaagtag tccttttgtc gctggattag
    18001 cgatgcttcc cggcgcagcg gttggtgctt tgctttctcc tttttccggg aaaatgctgg
    18061 atcaatatgg tccgaagaag ccaatttttg ctgggctgat tctttctttg attggttggc
    18121 tagcattatt cctactcata ggacacgctt cagtgggtgt attgattgct tgtcatgtgt
    18181 tttatatgat cggtatcgga ttatcttaca gcaatatgat gaccaccgga atgaatgcac
    18241 tgaatgaaga actacaagga gacgggaacg caatgttcaa taccttgcag cagttttctg
    18301 gcgctgtggc tacttcatta gtagcggtca ttatcaatta cgtccagcat catacttccc
    18361 ataactatga agtgtctact acattaggat caaaagcagc attaggcgtc ttgttgctgt
    18421 tgcttctcgt aagctttgct cgattcgcct attatctgtt ctttgctaaa aaagcgtaga
    18481 ttccatatac ggcaggtgtt gcatctgccg tttttttgtg tgaaaatggt tgtttgtaaa
    18541 aggaggcttt tttatggaaa aaattgccat tatcggtggc gggatcattg gtatgaccct
    18601 agccagttac ttagatacat caaaattcaa tgttgcttta tttgatgaag ggacaggaca
    18661 ggcaactaaa gctagcgctg ggattatctc cccttggttg tcgaaacgca gaaacaaaca
    18721 atggtatcgt ctggctaaag acggcgctgc ttttttccct aaacttataa aagatttcca
    18781 acttgatcaa acgatttacg cacaatcagg gacacttttg cttcgacctg aaaaagattt
    18841 agctgaattg gctgttcttg ccgagaaacg aaaagaagaa gcgccagaaa ttggaaacat
    18901 cgaatggctt tcacctgaac agacatctca agcacttccg ttattgaaac cagcagtttc
    18961 actgcgtatc agtggtggtg gtcgtctgga tggaaaagca tatttagaac atcttggtca
    19021 gcgtttagca caaaaacaaa tggagcagat ccaggcaaaa gtcactttgc ataagaagag
    19081 cgattcttgg ctcatcaagg gaccgtttgg agaatacaca gctgatcgtg taattttgac
    19141 acctggcccg gcacttaagc ctttattaca agaattaggc tatgctgcag atatacgtcc
    19201 gcaaaaaggg cagctcttag catttcagac atcttataag atgagtcaag actggcctgt
    19261 tgctatgctt gacggagaag ctgatttcat tcctttcatg gatggaaaga tccttttagg
    19321 tgctacccat gaaaacgatc aaggctggga tttggctcct actagcgaag cttataaggg
    19381 attgactaag agtacagaac agttcttgac tgatccagat cagataaaaa actggccata
    19441 cgagttccgt gtaggtaccc gtgcttatac gtcagatttt tccccatttt tcggcgtact
    19501 gccagatgat ccaagcttac tggctgctag cggtctaggc tcttctggct tgaccacagg
    19561 tccttatatc ggctatctat tagccaactt cttaaatact ggtcattggc ctacggaaga
    19621 ttatcaaaag gacatctcca cctatattac gaaaaatcca tcatagaaaa acagcttttt
    19681 caagtaagga tcccgagcga tattgcgcat ggattacttc ttgaaaaagc tgttatttta
    19741 gttcaatttc tgaatcaaat tctccaaact ttcttttgct tcatctggca agagatcttt
    19801 tcctatctga gcttcaatga atcgtaaacg aatatcaagt cgttgtttca ccagattttt
    19861 ataggacgta tcctcaaaag aaacaggaaa atctggtata ggtaaatagt ggatgtcttc
    19921 aaatggacca aacgacttga attctgccgt ttgttgcacg attctttcaa cgattgacaa
    19981 tgtcgtttca ggtttgattt ttttatcatc gaaatatcca gtattcagga catagcaggc
    20041 tgtttgctgt ttcaaaaaca actctcgaaa agcttgatag tcttccctaa gaggatagac
    20101 acggaaagga ttagcaaatg gttcaatgac tagctcatct agctgtcttt ttgtagtgat
    20161 attttccgcg tctgatcgtt ttgttgctaa tgttgcacca aaagacgctg ctaaaactgg
    20221 gtcattgatc ttgataattg gcggaagcga atcatctttc atgatccaaa aaacagcatc
    20281 gatcttttcg ctaagatgat cgactcggtc gggtgtcgca taacgtgatt tgactgtccg
    20341 cccatttccg tttcgtatat ctcctgttac taggactttt tctcctttac ttgtacatgt
    20401 cacgcccaca ttctgacaag taaggaaaga agaaatggcc ggatcatcag acggatagtc
    20461 ttgtgtttta tcaaagtaag aaggttccaa ggcagtagca aaaccatttt ttcgagaaat
    20521 aacgaaagca tcgtcatgca agatcgaaac agagtgacct tccgtatgtt ttgctaaggt
    20581 aatcgttgat tttccagaac cggacaaccc aaatacagcc atcgtatagg tctggtcatt
    20641 actagcatat tgtttcatcc caccgtggca ggcaataaag ccattgcgat ttcccaatgc
    20701 ccaagcaagc gtcaaagtcg ctttttttaa ttctccaaag taacgtaatc ctagcacagc
    20761 cgctgtatta tggagagcat caaacaaaat gagtccattt ggaaatgttt catgtgtcca
    20821 ttcgggatca caaaagatat aaagatctcc ctcatcaaag gaaacagact tttgataagt
    20881 ctctaagatc ttcgaattcg ctggctgaaa attcagcaaa taagaataca aattgttctc
    20941 aaatccttct ggaatcagca aatgactttg cacagaaaat tcttcagata ggccaacgat
    21001 tacttttgta tggtaaaact ggcgcttgcg actttgatac acagcttctc ttaaaatttt
    21061 ctcgtatttg ctttgttcct tgtctaagtc ctcaaagatc cgccgagcac ttgctgttct
    21121 tcccacaatg cttccatcat tgaatactag tgtcctaaca tcagctggta aatctagttc
    21181 ttcagcatgt gagattgtta aatctgtgat gatcgttcct ggactccctt ttgctagttg
    21241 ataagctgtc ttcctatcaa aaacttcatg aacattatta ccgtaaaatg tcgtttctac
    21301 aatcgatcga atactggata acagtggatt tccacttgtg atttcgttta attcgagact
    21361 ctcttttgta ctcatggctc ctcctcattc cctaaacaaa aaaacgtttt ctttattata
    21421 cattattcga gtttttattt gtactgctgc gctgtgggaa gcaaacgagt tatttttcac
    21481 acaaaaaaac cgagaacttg aataaacaag tttctcggtt tcatcatttt ccttttatcg
    21541 gcttagtacc agccgtttgc taaccagaat gttttagcag catcccatga accataacga
    21601 gatgttacat attgttctgc tacacgttct tgattggcag ctgaatagtc gccgtttaag
    21661 tatgaagcat ctaattgata acggccgatg taacgtccgt tagtagctgt gtatgaaccg
    21721 cctgattctt tttgtgcgat ccattctttt gcagagcttg aagttggcgc tgttgcagca
    21781 gctggggcag caggagctac ttcaactgtt tcaacaggtg caacgtatgt atgttcttgc
    21841 gttgctgttt cagttgtaga tgctgtattt tctgttgttt tgttttctgt tgttgctgaa
    21901 gaatcatttg aagttgggat tgttaattgt tcaccaacat aaatgcggtt gatgtttgcg
    21961 atcgaatttt tttcagcaat tgcatcgatc attgagttgt ctcctgcaaa tttttgtgag
    22021 atcttagata atgaatcacc tgattttact gtataaactt catcagcgtg agcatttgta
    22081 gttcctacaa acattgcagt agcaccagca gcaaaagttg tacctaaaac aattttctta
    22141 aatgaattca tattaaactc ctttttttct ccgtttttta ttctttcgac gctattaact
    22201 tta
//
LOCUS       DACCXO010000041        22184 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.042, whole genome shotgun sequence.
ACCESSION   DACCXO010000041 DACCXO010000000
VERSION     DACCXO010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.042"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            254..2089
                     /gene="tdc"
                     /locus_tag="IVY70_001410"
     CDS             254..2089
                     /gene="tdc"
                     /locus_tag="IVY70_001410"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP7958546.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2394..3872
                     /locus_tag="IVY70_001411"
     CDS             2394..3872
                     /locus_tag="IVY70_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7958547.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3838..5169
                     /locus_tag="IVY70_001412"
     CDS             3838..5169
                     /locus_tag="IVY70_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7958548.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5283..7835
                     /locus_tag="IVY70_001413"
     CDS             5283..7835
                     /locus_tag="IVY70_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP7958549.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7842..9281)
                     /locus_tag="IVY70_001414"
     CDS             complement(7842..9281)
                     /locus_tag="IVY70_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7958550.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9256..9438
                     /locus_tag="IVY70_001415"
     CDS             9256..9438
                     /locus_tag="IVY70_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958551.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9627..9989
                     /locus_tag="IVY70_001416"
     CDS             9627..9989
                     /locus_tag="IVY70_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958552.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10176..11033)
                     /locus_tag="IVY70_001417"
     CDS             complement(10176..11033)
                     /locus_tag="IVY70_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7958553.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11153..12064
                     /locus_tag="IVY70_001418"
     CDS             11153..12064
                     /locus_tag="IVY70_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP7958554.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12071..13717
                     /gene="mdcA"
                     /locus_tag="IVY70_001419"
     CDS             12071..13717
                     /gene="mdcA"
                     /locus_tag="IVY70_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP7958555.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            13819..14118
                     /locus_tag="IVY70_001420"
     CDS             13819..14118
                     /locus_tag="IVY70_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP7958556.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14108..15784
                     /gene="mdcD"
                     /locus_tag="IVY70_001421"
     CDS             14108..15784
                     /gene="mdcD"
                     /locus_tag="IVY70_001421"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP7958557.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15788..16381
                     /locus_tag="IVY70_001422"
     CDS             15788..16381
                     /locus_tag="IVY70_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP7958558.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16405..17481
                     /locus_tag="IVY70_001423"
     CDS             16405..17481
                     /locus_tag="IVY70_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP7958559.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17506..18375
                     /gene="mdcB"
                     /locus_tag="IVY70_001424"
     CDS             17506..18375
                     /gene="mdcB"
                     /locus_tag="IVY70_001424"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP7958560.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18486..19250
                     /locus_tag="IVY70_001425"
     CDS             18486..19250
                     /locus_tag="IVY70_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP7958561.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19739..21832
                     /locus_tag="IVY70_001426"
     CDS             19739..21832
                     /locus_tag="IVY70_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7958562.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21857..22159
                     /locus_tag="IVY70_001427"
     CDS             21857..22159
                     /locus_tag="IVY70_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7958563.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7181 a         3789 c         4725 g         6489 t
ORIGIN      
        1 acagtcattt tatttgtttg ttcgcttttt ttaggttatc tattttagaa gactgattta
       61 ttgaaaaatt gttcgctttt ttgcgttgaa ttatcagaaa aatccgactt tatttatttt
      121 tgtttgtaaa aatctacgtg gatttgatgt tataaaaaaa aaattctcat ttttggcgct
      181 atagtgaaga ggtgttacag ataaacacat gtatctgcaa gacattgacc aacaaaatat
      241 ggagggaaga aaaatgaaag atatggatat caaggccgtc tttataggcg acaaagctga
      301 aaatggacca gtttataaaa tgttgttgaa caagatggta gatgaacatc ttggatggag
      361 agaaaattat attccttcag atatgcctgc aattagcgaa ggagataagc tcactccaga
      421 ttatctagcc acccgcgacc atatgataga ggtcttggat gaagtcagtc agcgattacg
      481 cgcaggatcg atcccatggc attcagcagg ccgttactgg ggacaaatga atgctgaaac
      541 attgatgcct gctttattag cttacaacta tgcgatgctg tggaacccaa ataatgttgc
      601 attggaatct tctatggcaa cttcgcaaat ggaagcagaa gtaggacagg attttgcttc
      661 attattcaat atgacagacg gatggggaca cattgcagca gatggttcta ttgctaatct
      721 tgaaggatta tggtacgccc gttgcatcaa atcgattcct ttagctgtca aggaagtgct
      781 gcctgaaaaa gtgaaaaaaa tgtctgagtg ggaacttttg aatttatctg ttgaagaaat
      841 tttggaaatg accgaaagct ttacggacga agaaatggat gaggtcaaag cagcttcttc
      901 ccgtagcgga aaaaatatcc aaagattagg gaaatggctg gtccctcaaa caaaacacta
      961 ttcttggatg aaggcgttag atatttgcgg agtcggctta gatcagatgg ttgctatccc
     1021 agttcaagag gattatcgaa tggatattaa tgctttggaa aaaacaattc gtgaattagc
     1081 tggacaaaaa atcccaattc taggtgtggt tgctgtcgta ggaacgacag aagaaggtca
     1141 agtagacagt gtagataaaa tcgtccaatt aagagaaagg ttaaaagatg aagggatcta
     1201 tttctattta catgttgatg ccgcatatgg cggctatgcg cgttcactgt ttctaaacga
     1261 agcaggagaa tttgtgccgt atgcttcctt agctgaattc tttgaagaac atcatgtttt
     1321 ccaccactgc gtaacgattg acaaagaggt atatgaaggg ttcagagcta tttcagaagc
     1381 ggattctgtc acgatagatc ctcataagat gggctatgtc ccttatgctg ctggaggaat
     1441 cgtaatcaaa cataaaaata tgagaaatat catttcttat ttcgcgccat atgtgtttga
     1501 aaaatcagta aaggctccag atatgttagg agcttatatt ttggaaggat caaaagctgg
     1561 agcaaccgcc gccgcagtct ggacagcaca tcgtgtattg ccattgaatg tgaccggata
     1621 tggtcaattg attggggctt caatcgaggc agctcaaaga ttcagagaat ttctcgatca
     1681 tctgactttt actgttaaag gaaaaacaat cgaagtctat ccattgaatc atcctgattt
     1741 taatatggtc aattgggtat tcaaagaaca aggctgtaca gatttgaacg ctatcaatga
     1801 attaaatgaa aaaatgttcg atcggtcttc ttatatggat ggcgatgttt atggtgaacg
     1861 gtttattact tctcatacta catttacaca agaagattac ggtgattctc cgattcgttt
     1921 tgttgaaaga atgggtctaa cgaaagaaga atggaaaaaa gaacagaaaa tcactctgct
     1981 acgtgcggct atcatgactc cttatttgaa tgatgatcga attttcaatt tttatacaaa
     2041 aaaaatcgcc aaagcaatgg aaaagaaact aaacgaaatc atccaataga actgattttt
     2101 gtgtgaaaga ataacaagag acatatggag aacagacaat tatttgcttc atcaaaacaa
     2161 ttgtatttcc ccctgttttt catatattcc tcgccaagat attctttttt ataaaacgtt
     2221 ctataagttg gatcgttcgt ttcatgtatc aaacccagtt cactaagatc gttcaatgga
     2281 tgtttcggtg atactgggtt ttgtactgaa tcgatgaaac cttgtcattt ctgttcttga
     2341 acaaaaataa aagaaaacaa aaacatatgt cgctattgaa agaaggaata aagatgaaag
     2401 atactggttc atcaaagcaa atcacgacca tgacctttat cggaatgacc tgcgcccttg
     2461 tagcaagtgt acgaaatatt ccagatgtag cagcaacgag ctggacgatg attttttaca
     2521 tgctggttgc agtattattg tacgcttttc caatctcatt gatctctggt gaatttgctg
     2581 gaatgtttcc ccaaaaaggt ggtccagaac tttgggtatc gaatgctcta ggaaaaaaat
     2641 gggggttcgt tgtctcatgg ctgttatggg tccaaatgtt tcctgggatg gtgatggtcg
     2701 cttcagcact cgctccgttg tttggtaata ttatcgataa tgtgccgctt ggtttgaaca
     2761 gcaaatttac attagttgtc attttagtcg tttattggat catcactttt ttgaatttga
     2821 aatttgatat ggcaaaaatc ggcggaaaag tcggtgtttg gttaggattg tacgtgcctt
     2881 tgacgatgat gctcttatta gggtttgctg cgtggatcaa agttgggatc gtaccaaccg
     2941 gaacattagg cagtttttca tgggacaaat taataccaga tactacgact gcatcctcat
     3001 ttgtttattt tgcgccgatc atgtttattt ttactggtat cgaaatgtcc tctgtgtata
     3061 tcactcgatt ggaaaatcca gttaaaacgt atattcgcgg agtatttgcc gcgttaattt
     3121 ttatattttt cgtcaatatt ttgaatgctt tagttgtagc aaatgtcgta ccaaaaggac
     3181 aaatggaatt gaataatatc gcgcaatcta tttcgattta ttgccaaatt cttggtttgc
     3241 cacatcttat tgtgaatctg tttagtttac ttgtcttcat tggtgtagca gtccaattat
     3301 ctgcttgggc ctcaggacca gcaaagacag tgacagaaag tgctagaaaa ggtgcgtatc
     3361 caccaaaatt caatttttgg aagacgaatc aatttgacgt atccaaatcc gttattttga
     3421 ctcagtctgt cattatctca atctttgcac ttttttattt gctgattcct ggcgtcaatc
     3481 aagcattttt gatgctcgta aattcaacta ctgttatcta ctgtatcgtc tatgtcatca
     3541 tggggatcgc agttttgaga ttgcgttata cacatgcaaa gttgaatcga ccatttagaa
     3601 ttggaaaaaa agagaaaaag agcaatcttg gagtttggac tgtcgttatt gttttatttg
     3661 cggcaatcgg attttctgtc ggactgacga tgaaagctgg aacatggatc aatctaattg
     3721 cagtcacagc catcagtgtc attttgtttg tagtaccact ctgtattgaa aaaataaaga
     3781 aaccttcttg ggaacaggaa gtcgccatgc tgcttgaaaa agaaaaaggt gaacttgatg
     3841 gaaaaaccgg caatgaaacg gactctaact aatcgaaata tccagttaat cgcattaggt
     3901 ggcgcaattg gaacaggatt atttttaggt tcagcaaagg caattgcatc agcagggcca
     3961 gctgttattc tcgtttattt gatatgcggt ttggctattt atggtttgat gcgggccatg
     4021 ggtgatttat ttttagcaaa tagtacattt aactctatca gtgatttcgt tgactattat
     4081 ttaggcaaaa agtgggcatt ttttgtcaat tggacttatt ggctttgctg gcttggagct
     4141 ggacttgcgg aattaacagc aataggattg tatatccatt tctggtttgt acatgtacca
     4201 gctttgataa gcggaggtag cgcactaatg atcttaatgg cagtcaacct tgttgcagtc
     4261 aagtggtttg gtgaattgga atcttttttc tcaatcatca agatcttggg gattttcctt
     4321 tttatcttat tgggtatagg attgagtgtc tcagattttc atacccagtc tatcggaatc
     4381 aaagaaaatt ttggacagat cgatgaatta ctaccgtttg gctttgctgg aatggtagtc
     4441 tcatttccta tggttttatt cagttttgct ggaattgaaa tgatcggctt aaccgttggc
     4501 gagacaagtg atccgcaaaa gaatttgaaa aaagcaatca actgtctgcc ttggcgtatt
     4561 ctctttttct atataggaac gattttgtcg attttatttg ttgttccatg gcattgttta
     4621 gatttaaaag aaagtccttt agtgactatg ctgcaagtga tccattgcca aggcggagca
     4681 gcaattgtca atctaatgat tttggttgct tctttgtcct cagcaaatag tgcagtctac
     4741 agtactagca ggattttata tcaaacagca aaagaaaaaa gtttgcccca ttggttttgt
     4801 gcgctttcta agaaaggcgt acctttacac ggtattttaa ttacaggaat gttatttttt
     4861 gccggtcttt gtctacattt ttttgtagct gatagtcgat tgttttttca gcttttggca
     4921 ggcatgacaa cgagttgttt tctatttgtg tggtcttcga tcttgtgtgc acatgttcaa
     4981 tttattaaag aaaagaaacg gaacactaca caaagatata aagatcttgc tttgttggtg
     5041 ttttttactt caattgcttt cagcttgtta ttcgaaagca cgacacgctt tattcctttt
     5101 ttattggtta tctggtttgt attcttaaat tttgtatata aaagaataag caatagaaat
     5161 atcagttagg cccagataca ttttgcaaaa cgaattattt cagacccccc tgaaataatc
     5221 gatacgcctg aaaaaacttc cctgcgtata ataacagtat caattaaaca ggaggggata
     5281 ggatgaactg gtacagattg acggtagaag atgtattgaa aaactatcaa acatcaacag
     5341 aaggcttgtc gcaagaagaa cgacagaaac gcctgactga tcaaggatat aatgaaataa
     5401 aagcaaggca gcagacaaaa cgatggaaaa aaatagcaaa gcattttact gatctgctga
     5461 tgatcgtgtt gatcattgcc tcttttttga aatttgcttc tagtgaatat attgaaggaa
     5521 gtattatttt attcgtagtg atcgtcaatg gactcgttag ttactggcaa gaaagaaaag
     5581 cggaagaatc attgaatggt ttgaagcagc tgatgggaca agaagcgata gtactatcaa
     5641 acgggatgca agaaaaaatc cctgcgaaag aactggtaaa aggagatgtc attgtcttac
     5701 gtgccggaga tgtcgttcca gcagatatcc gattaatcga agcacatgat ttattagtgg
     5761 aagaatcgat tttgacggga gaatcagaag catctgaaaa aagtcatgcc gttttgacag
     5821 aagaagaatc gatcggcgac cagaaaaata tgggtttttc aggaacgctt gtacaatctg
     5881 gttctgcagt cggtgtcgtt gtagaaacag gaaacacgac agaaatcggt aaaatcaatc
     5941 aagcattgca atcggtcgaa caacaaacga cgcctctgat taaaaaaatc aaccgattaa
     6001 acaaacaaat ttttcaaggc attctttgtt tgagcctatt cttagttatt tttacgacat
     6061 ttcgatatgg tatggattgg catatccttc tttcggctac gattgctttg gtggtgtcaa
     6121 tggtacccga aggattgcca gctgttttga ccatgattct atccgttgga gtacatgaaa
     6181 tggcaaaaga aaaggcgatc atcaaaggac ttccttcggt agaaacatta ggttctatga
     6241 cagtgatctg ttcagataag acaggaacat tgacaaaaaa tgaaatgaca gttgtggatg
     6301 tagctgcaaa agaagaagcc tgcgtgttgt ccattatgaa aaactgtcag gaattaaaaa
     6361 caaaagaaga acagaaaaca gaaaatttaa gtggcaatcc gacagaagtt gcgctgattc
     6421 gacacacaga aacagccaat ctacctttga aaaaaataga agcaaagatt ccgttcagtt
     6481 ctgaatacaa atatatggca acgatgcatg cggaggaaaa aggagcggtg atttacgtca
     6541 aaggtgcacc agaagtatta tttgctaaat caactctttc ttctgcagaa caagaagcat
     6601 ggagtcagac agctgcagaa tttgcgagaa aaggtcagcg tgttttagga tttgcttata
     6661 aaaaagtaga ttcgaaacag gaactcacgc atgagacttt aacccaatta acttttgcag
     6721 ggatcgccgg attgatcgat ccgccaaaag aaagtgcagt caaagctgta aaggaatgcc
     6781 aacaagcagg gatctcagtg aaaatgatta caggggatca caaagacaca gcaaaagcga
     6841 ttgcggaaca aattggctta aaacatactg ccaaagtttt agaagggatc gatttggatc
     6901 ttatgtctga tgaagagttg attcagcaag tgccaatagt cgatgtcttt gctcgaacga
     6961 caccagaaca taaactgcga attgttaaag ctttgcagaa aaatggagaa atcgtcggaa
     7021 tgaccggaga cggcgtaaat gatgcacctg ctttgaaaag atccgatgta ggaattgcta
     7081 tgggaatcaa aggtagtgaa gtaagcaaac aggcagctga tatggtttta ggagatgata
     7141 atttccatac gattgcaaaa gccgtaaaag agggccggcg gattttggat aatttgcaaa
     7201 aaaccatcaa tttcttcttg ccaactgcat tagctcaagg attgatcttg atttgggcac
     7261 ttatgctgaa tcgcccgcta cctttgtccc cagtacagat tttatgggtc aatatggtaa
     7321 cgacaatcac tttatcttat gcactagggt tcgagtcgcc agatccagaa atcatgaagc
     7381 gcccaccaag agatccaaag caaggaatcc tatctggtta tcatttgttt cggatcatct
     7441 atgtctccct actgatcatg gttcctgcct atttattggc tatgcagttt gaagggcaaa
     7501 gcttacagca aaccattctt ctgcaaaata tcgtcttagc tcaagctgtt tacatgatca
     7561 attgcagaga gttgtccaag ccgtcattga accaaggtct tttgcaaaac aaagcgcttt
     7621 ttctttcatt aggtatcctt gctttattgc aagggatggt tatttttcta ccagtcggcc
     7681 agcagctgat cggtacagta agtttaacga tgacacaaca gttattgatt ggagcaaatg
     7741 tgattcttct attcttagtt gtagaaatag aaaaagggat catgaacaaa ctgtttcgca
     7801 aaaaagaaaa aacagctaga gaatacagtc gctagccgtt actataaaaa agttttgaaa
     7861 gtgagttcag tcaaacgatt gagctcattt ttcgttggaa tagaagaaat gtatacaata
     7921 gggatatcta gagaatctag agggaaattg gaaatcaaga tatctgtttt ttcaaaagaa
     7981 atagtggaaa gatcggttag ttcgataggt aagaagcaga cacgttttcc taagtaatct
     8041 tttaattcct gctgcaagaa cgccttccat gaaggttctc cttggaaaat aaaatagaca
     8101 ttgaacaaat tcggtttgat agaaaaaatc gtttgttgga tcagtagaga caagagttgg
     8161 agaaagtaat ttggttcatt gatttctatt tcttttttta gatcgttcag taaattctct
     8221 ttctgttgat acaagtgtgc atattgctga tcatatgttt ctaaaaaacg atggtattga
     8281 cggtccaatg cttcaatcaa tatcggcgat tcatgatatt ttaatatgag gagagccaag
     8341 ttatcagtca gagatgtttt ttttagttga tcaagagaca gctctgaagc tagttcctct
     8401 acaaacgttt cgaggacctc taaaaatgga gaatgatgca ggcagcgagc ggtttcttgt
     8461 ccatagttat tgttgtaatt ccaactttcc agaaagcaga aagaagcaaa agaaagctca
     8521 gtgtctttaa gataaatacc ttccgttaaa tacaattttt tcaatgcaga gtcatacagg
     8581 gaggagagtg tgctgttggt atggatagaa gaatctaatc ggcaattcgc ttttatacgg
     8641 atcgtattga taaagaacca acatgttccg aaaatctctt ctgtttcaac cgctagttca
     8701 gtttgactga ggtttcttaa aaattgaaaa taatgggatt catggatcga ataatcctcg
     8761 aaaaaatagt tgttatgagt gaaaggaatc agcaatcgat gataaaaagt acgaatcgca
     8821 cgctcttttc caagaataca agctgtatat ttcgtcaaat cgatctgcaa gtcgtaactt
     8881 gctaaacgct gattgatttt agtcacatgt cttttcaatg tcccataaga aagcccatgg
     8941 tcggctagaa aatcagggac agcaactttt tttgtaaaaa gcagattttt cagcagttcg
     9001 acactgactg attgtttgaa aaaaatctcc caaaggtcat ttgttaataa attttgctgg
     9061 ttttgcaaac tgattcctgc ttgatcgctg atgatgtccc agccttcggg cagctgagag
     9121 cgaatagatt gaaggtcaga aaaaatcgtt cgttcggttg tccctatgat ttcagctaat
     9181 tcatttacgg ttatctttgt atgattcagc aattgttcta ctaaagaaac atgacgtcga
     9241 atatcttttt cggtaatgat ttttttcatt agtgaataca tcaatcagac tcccttctat
     9301 tctaagaata cggagaaaat aaacggtggt caagtttcaa ctgatttttc aatcattgtt
     9361 tatttttctg gagcatttac tgatgataag aatttcatca tgaacttgaa aaatgatacc
     9421 actaaaatca aaaaataggt gggcaaacaa taataaacat tttaatagta gatggtagca
     9481 agaccaacac aggcggattg aacatttaag agttgataaa aaagaaaaat atcgaagtat
     9541 ttgattagca actacttgta ttcatttttc tattcttacg tggtatcttt gaataaagcg
     9601 aatcaataag aaagagatgg aagcagatgc taacgaaaaa agaactaatc gatcacatca
     9661 atgaaacata caatttgtct ccagaatata tttttaaaaa atttcctgat tattgcgtat
     9721 tcagacatca aaaaagcaaa aaatggtttg gggtcctgat gtctgttcca gaagaaaaaa
     9781 tctatgatga tgcaagtgac cataggatca tcaatattct tgatttgaag gtcgatcctg
     9841 aacttggcgg aattttgcga ggaaaagagg ggtattttcc agcctatcac atgaataaag
     9901 aacattggat ctctattgat ctacgtaaaa tagaaaagct agatgaacta gcagatttga
     9961 ttgatggaag ttttaaatca acgcaatgaa tgtctaggac aatagagaag aggttgtgac
    10021 agaaatgttt agcttcaaga aataagaagg aatttacgaa aattgcttct caaatttttg
    10081 ggaaatttcg gcttattttc taagaaatta cttctggaac accgtttgtt cgaaaaaaga
    10141 gctgcgacaa gaattttgtc ccagctcttt tttttttatt ttcctaaata aaattcccgc
    10201 atcgtttttg caatcatttt gatcaaagga tgatcttctg tgaagctaga agataaataa
    10261 attgttcgtg tcaagggcaa atctttgatt tcccgcaaga cgaaaggatc atttttatcg
    10321 taagaccaag aaacttttgg gaaaaagcta acagctaatc cttgcttgac aatagaacgt
    10381 aatgtcgcag gatcatctgt cgtcgaactg atcgtcaatt cgatactttt cgcttcaagt
    10441 gctgcatcca gtgtatgacg gaaggcgttg ctctttgaca gcatcaagat gggggtgcgt
    10501 tggatatctt ctaattgcag atgttctttt ttagcaagag gatgtttctt aggaatggcg
    10561 acataaacag gttctttcat gataggaata ttggtcatgc caggcttcat ctccgaagtg
    10621 atgtacagta caggatcggt aatatcatct ggtaaatgat gttgcatgat cgttaattgg
    10681 atatcaggat gcttttccat aaaaattcgg accaagttcg gaatagaaac agaggctacc
    10741 tctaaataca atatgacgga agtaacgatc ttctcattga ccgaagacaa taatttgatt
    10801 ccttcttcta atgtgtctaa tgtcttgact gcatatggat aaaattcttc tgcataaacg
    10861 gtcgtctcaa tgtttctgcc gctatggtaa aacaaaggga cacctaattc gttttccaaa
    10921 tgtttgatcg cacggctcaa ggcaggttga gaaataaata attctttggc tgcttgggta
    10981 acattttttg tttcgaatac ttttttaaaa tactgcagat gaagcaattt cataattatc
    11041 catcccatcc ataccttttt gttatggttt tttctttttt tggcattttt tattatagca
    11101 gaaattgact aagatagcag tggaaaaagt aagtgctgag gaggcgaggc tcatgagtac
    11161 aggtttctta tttcctggac aaggcagtca gaagttaggg atgttagcgg atatccctga
    11221 aatgtacatg gaacgtttag aaaatgtgac ggggtatcgg ttagttgata aagaagaaaa
    11281 ctatcaagat acggttttta tccagctagc attactgaca aaagcagtat tttatttaga
    11341 tgaaatggaa aaaaacggta ttcagcctga tattgcagca ggacattcga ttggtgcttt
    11401 ttgtgcagct gttgcttgtc gatctttgag atttgagaat gcagcggaac tagtctatca
    11461 ccgcgcagtc ttaatgaaag aagcttatcc aagcggttat gcaatgggcg tagtagtagg
    11521 tgtgacgaga agcgaagctg aggaaattgt agcaaaaaca tttgatcctg accatccagt
    11581 ttatctgtca aatgaaaatt gtccgatgca acatacgtta tctgggagta tcgaaggttt
    11641 atccgttacg ttggcagaag ccaaacggca ccaagcgcaa acagcgaagc tgctgaaagt
    11701 ccctgtgcca tctcactgtc tcttgatgaa tgatacggtc aagaaattca agccgtatat
    11761 gcagtctgtc catctatctg atgctagatg tttgtatata aaaaatacgg atggaagagc
    11821 cactcaggac gcagaagaga ttcgtaaaga tttgttggaa aacctagctt tccctgtgca
    11881 gtggaaccaa atgatggact tggcaaaaga attaggaatg gatctgacga ttgaatttcc
    11941 ccccggcaat actttgacaa agctgattca tacaaaattt ggtgtagata aagggatccg
    12001 tacgatcaat ctagaccaac atggtattga tgacgccacg tttctatatc aaaaatggag
    12061 atgactaatt atggaaaaac caaaccgcaa ctgggcttct aaacgcgaaa aaaaagcgca
    12121 gcgattggaa aaaatcagca acttgctcaa tggcaagtat gtagaaactg aaaaaatcgt
    12181 agaagtaatg gaaaaactga ttgctcccgg cgacaaagtt gtattagaag gcgacaatca
    12241 aaaacaagca agttttcttt cagaaagtct tgaaaaagta gatgcagaaa aagtgaatga
    12301 tctgcatttg atcatgtcta gtatctcacg accagaacat ttgaatattt ttgaaaaagg
    12361 aatcgcaagc aagatcgatt tctcttatgc tggtgcacaa agcttgcgtg tcgcccaaat
    12421 gattgaagac ggcacaatga aattaggtga tatccatact tatttggaac tttatggacg
    12481 cttattcatc gatttgattc caaatgttgt tttagtagct gcagataaag cagataaaga
    12541 cggcaacttg tacacaggtt tcaatacgga agaaacacca acaatcatcg aagctgcagc
    12601 tttcaaagat ggtatcgtga tcgttcaagt taacgaagtt gtcgattctt taccacgtgt
    12661 agatatccct ggtgactggg tagacgttgt cgtgaaagca gataaacctt atcaattaga
    12721 agctttattc acacgagatc ctcaaaacat cacagaattg caaattctaa tggcaatgat
    12781 ggcaatcaaa ggaatctacg cagaacacgg cgtacaatct ttgaatcacg gtattggatt
    12841 caatacagca gctatcgaat tgttactgcc aacttatggt gaatcactag ggttaaaagg
    12901 gaaaatcgcg aagaactggg tattgaaccc acatccaacg atgattccag ccatcgaatc
    12961 aggttgggtt gaaagcatcc atagttttgg tggcgaagtt ggtatggaaa aatatattgc
    13021 tgctcgccca gatgtctttt ttgtcggtaa agacggcacg atgcgcagta accgaacatt
    13081 atcacaagct gcaggacaat atgccatcga tatgtttatc ggttcaacct tgcaattaga
    13141 ttacgaagga aactcttcta ccgtaacaaa aggacgttta tctggttttg gcggtgcgcc
    13201 taacatggga cataatcctg gcggtcgtcg tcattctaca ccagcatggc aatcacttcg
    13261 cgacaatgat gatccattag ggaaaggcca aaaattagtt gttcaaatgg ttgaaacata
    13321 tggttcaaat aaaaaaccag tctttgtcga agaattagat gcactagctg ttcaaaaaca
    13381 agcaggctta gccaatgcac cagtgatgat ttatagcgaa gatactacac atatcgtgac
    13441 agaagaagga attgcttatt tgtacaaagc aaaaacaaaa gaagaacgac aagcagcaat
    13501 tgcagcaatt ggcggtgtta cacctcttgg aatgactagc acgaaagaat cactagatag
    13561 cttgcgtgca gccggcatcg tgaaattacc agaagatcta ggcatcaaac gtagtgaagc
    13621 gaaacgttca ttattagctg cacaaaccat cgatgatttg atcgaatggt cagatggcct
    13681 gtatgaacca ccaatgaaat tcagaacttg gtcataaaat tggcgagaaa aaccttttgt
    13741 cgccatttac ccagatggaa gaagaaagac gaataagcag aacgtgtatg ctctgtttta
    13801 caaaaagggg agagatccat ggaacaatta gcttataaat atccaacgaa acagccgatc
    13861 aataagaaaa tccatgtagg agtggtcggt tcaggagatt tggaaatctt gatgtttcca
    13921 acagaaaaaa cagaatctac tgtaaaaatt tgtacaggaa gcgatggctt cggcgaagta
    13981 tggaacaatg tattaacgag attttttgat cgttatccaa tttcagcaga tatcgtgatc
    14041 aatgacttcg gtgctacacc aggagttgtg tacctacgat tgacgcaagc aatggaggaa
    14101 ctttcagatg aaaaatagtt ttgtagaatt aaacgccaga gaacgagtga acgctctact
    14161 tgacaatgga cgtgaattga ttgatccatt cgatcaaatc atcgcgccaa gtttaccagc
    14221 acaaggaatc gtccctgaaa gtgatgacgg gatcgttgtc agccgtggca cgatcaatgg
    14281 caaacaagca gtagtaatcg ctatggaagg aaaattccaa ggtggcggta tcggtgaagt
    14341 ttccggtgca aaaatcgttg ctgcacttga acatgtgtta gaagaaaata aaaaaggaaa
    14401 tgagatcttt ccagtcattg tactagatac aggtggtgtt cgtttgcaag aagcaaacta
    14461 cggactgtta tctatttcag aaatcggaaa tgtaatcatt gctctaaaac aatatgtacc
    14521 agtcatcggt ttgattccag gacgtgtagg atcatttggc ggtatgtcga ttacttcagc
    14581 attaatgagt tatttaatcg caacaaaaaa agcgagagtc ggtttgaatg gaccagaagt
    14641 tattgaacaa gaagcaggcg ttcgagaatt tgattctagt gacaaagact tgatttggaa
    14701 tacattagga tctcgtcaac gggtagaatc aaaaatcatt gatgaattag tgactgatag
    14761 tgtagaagca atcaaagctg ctgtcatcgc agcaatggac gaacgaaaag acacgcatcg
    14821 cagtgaaagc tcagcatttt atttatcatt acttgattct ttagatcttt caaaaccaat
    14881 ggccattgac gattacaatg aagcaattgc taaccatcaa gcaaaatcaa ttgacttacc
    14941 agaagtagca gaaggaaatg aaccagctgc agcaagtgtt ggttacgatt ggtttgcggc
    15001 actaactggc atgaaaaatc caacttcttc tattagtact gtgtattatg gtaaatcaag
    15061 taaattcata gaagaagcca tcgtgacatc aatcgtacct gatcctaaaa acccgatgta
    15121 ccgtgtacga aacggtgaag ttggtttagt tgaaggtttc cgcatgggac aaattttgga
    15181 tcacgtttac gaagaagata aagacaaaaa agtgaaacgg ccaatcatcg ttgtgatcga
    15241 tgtaccaagc caagcatacg gctataacga agaattgatc ggtatccatg tggctttggc
    15301 taatagtgct gctgcttatg caaaactccg tcaagctgga catccagtta ttggattgat
    15361 tgcaggaaat gctatttcag gtgcattttt agcacatgga ttacaatcaa gccgcttgat
    15421 tgctttaaac aataaagaaa ttaccgtcca agcgatgtct aaagaaagtg ctgcacgtgt
    15481 gacaaaacgt tcggttgctg aaatcgaagc agctgctgaa aaagttccag ctatcgctta
    15541 cgatatcaat aattatacaa aactaggagc gatctatcat ttcctagaag atatcaccga
    15601 ccaagctgct tcagaacaaa atgtcagcaa agtcatcgaa gcaatcaatg aagccattca
    15661 agatgtccgc aatacaaatg acacgatgct gacaaatcga tacacaaacg aaacagccaa
    15721 aacattcgga cgtgtcgaaa caaataaagt ccgtgccaaa atggacgaag aatgggatgc
    15781 ataaaaaatg attcaagcac atgatattgt cctttttgat aaacatgaaa tagaagggtt
    15841 caccttgcct gaatgggtcg ttctaactga aaagatgttc ggaacagttc gtcgtgcgtc
    15901 gacggatcac gcagaaatac taccgattgg tttacgtgga cctgcccgca atcagagatg
    15961 gtcaacagaa atctcaaaac atgccgtcaa acaagtcatt catccgtggg acatcatcga
    16021 acaagaatcg tatagacaag agaaaatcat ggattatcct gtttatcagc aatttcatgc
    16081 tgctagaaac atattatcag gatgtaaatg gggaatcggc ggcagcttag gatttgaatt
    16141 atcgactggg attccggcag taaaagaaac cagtgatttt gatttgctgc tgtacgcaaa
    16201 ttcaccgatt gaattgccaa ttcaggtaat tcaatcgcat cctgcttttt ttgagcaatt
    16261 cgatacccaa gtagtgactc ggcaaggcgg tttttcacta aaagagtatt tgagagagcc
    16321 agagaaaaaa attctattga aaacagtcga aggacctaaa ttaaccaaag aactttggta
    16381 ataaagaaaa agagagagaa agagatgttt gaaaacaaaa aaatcaaaat caacgtaagt
    16441 gacttccaga aagtatcagc tgtaacggct gttatgatgc aaacgatatt gagctttgct
    16501 ttggcaaaca gccacagcgc aacaaataca gcttttcttg gtacctttta cgtcatcgtt
    16561 aaatacacag caccaatgtt tatttttgca attgtttata atatggtaaa aacaagtcag
    16621 catttgtctt attgggaatt tttgaaagaa aagttttttg aactagtcgt tccgtatctt
    16681 ctatggacga cggcttatct ttggctattt ccagcagtcc aacaacaaac tccgtatacg
    16741 gatactgcaa gctttttact gaaatatgtc acaggtgatg gagcgcctca tttatggtat
    16801 accgttatga tgctgcagat ccaattgttc atgccgtttt ttgtttggct cggttacaaa
    16861 gtatttgcta ataaaaaata tgtatggcca gtattgattg gttcaacgat tttatatgtc
    16921 gcatggtatc tgttctatca aacacaagta ttgactgggc cttatcatga cagctggtac
    16981 ttattagatc ggtttgttgc tagtttcatg atttatgggg tagccgcatt tgtttatcac
    17041 gaaaaagtct atcaatattt ggacagagta cgttatcttt tcttacctgt tgccttagtt
    17101 atcgcgttct tttctgtccg ttcattgctc gcacatccag gagacttgag ctttgcgaat
    17161 gcaccttact tgaatacgat ccaaagttta tacagtttag tcatcatctt tgctgtcttc
    17221 ataggcgctt ccaaaatgat tgtaaacgat tcgccaaaat tgccattgtt caaatggttg
    17281 tctgtttatg cttatcgtac gtatttagcc aatgtatttg ttttccaagt actgttatta
    17341 ttgtttaaag atagttggct gcagttacct tcaggagtca tgattcttgt agcttatcta
    17401 atgacggcaa gttgtgcatt tgctttaagc tggttacttc atatcatttg ggtagctatc
    17461 aaaaaaggtt tcagcaagta atagtaggaa gaaggagtga gtgtcatgat aaatgacgaa
    17521 cgaaaaaaag cagtgactca gcaactcgct gcgttggtag aggaagcatt gatcgcagaa
    17581 gtaacattaa gtccaaaacc tggattagtg gatgcactgg atgcgggtgc acattccgat
    17641 atggactatg ctttatttct taagtctgct aagacgctaa ctcctttttt tgaagaaatg
    17701 gctcaaatag catggcatca tgctattgat caggagttaa gagaaagaat tgctgagatt
    17761 gggcgaaaag cggaaaaggc aatgctgatt gctacaaatg gcatcaatac ccataaagga
    17821 gcgatttggg caatggggct attgatcagt gtcattgcac aaaaaatcag tatggaacag
    17881 cctttttctt tatcgaaaat acttgatgat gtcagcaagc ttgctgcttt tcctgataca
    17941 aaatatcgtc ataaaaaaac cactcatggt catagggtga agcaaaaata tggcgtaaat
    18001 ggtgcatatg aagaagcgat attagggtat ccgcatatca ggcttgcaat tcaagcagct
    18061 aaggagctag ataatgattc atatgtacag aaacaaatgc atatgttgtt agttttgatg
    18121 agttcgctag atgacacatg cgtattgcat cgcagtgatc agaaaacgtt agtggaaatg
    18181 caaaaacttg ctcatgctgc tagtcaaagt gctttaccaa atcgtccatt tacagcactg
    18241 ttgcactttt gtcaggaaaa ccaaatctct cctggaggaa gcgcagatct cttgtctgca
    18301 agcttgttca tgctgaagat agaggaatta tggactgccg acacatacat ttgtccgatg
    18361 ttactcaatg actaaataag acaaaattgc taaaaaaaca gtttatgcat ctggttattg
    18421 actcttcctt ttagcaaggc tatcataaaa atagtttttt gtaataaaag ataacgagga
    18481 agacaatgaa aaaaatcaat tatttagcaa tcattttgtt agtgggccta tgtttgtttg
    18541 ctctgccgac ccaaggattc gctgtcgcgg gtggtcatgg tggaggtaca tctagcggtc
    18601 gaagttcggg cagaggttca tttggtggtc gttcatatgg gggcagttat tttggcagtc
    18661 gttcttacgg cagagattct tatgggtatt atggtccagg tacaggtcct tctagttatg
    18721 agcaaacggg atttcttgca ttagctgttt tgtcagtagg aggggtttct gctttttcta
    18781 tcgtcaagaa taaaagacaa aagaaaaggc tgaaagcgga gatattgtcc cgcatgtcag
    18841 gaacggctaa agaaaagaaa gcacagtttc tggaaatcca aaagatattc atgaagatcc
    18901 aatccgcttg ggaagaagaa actcctgaaa aagccagaag ctgttataca gacaagctgt
    18961 ttgccgaaca tcagaaagtt ctgcaaaaaa acaaagcaga cggtgtaaga aatcatacta
    19021 aaaaaataac tattcaggaa gtaggaaatt atcgacagat ccacgaaaat agttttgcca
    19081 tccgaattga tttcagatgc tgtgattatt cagtcgatcg aaaagatggg cgattgatct
    19141 caggaagtaa gacacaacga cagtattttt ctcagatatg gtattttgat ttttctgaga
    19201 aagaaaaaag atggcaagcc gattttattc agccgattat gctggattga caggaaatat
    19261 ttatgaacgg agttgtgaca aaagccttgt cgcagctcct ttttctgaat aaacggtgtg
    19321 ccagagatag cctatcggca ataaggcacg attttgtaaa atatgggaag cgatttttca
    19381 aaatcgcttc ttatcgctca aggctgaaca tctctgtcac aacctctcca ctaaatggtg
    19441 tttaaaagct tgcatctgcg ataataagaa caaccaaacg aaaaatagga agcactattt
    19501 tagtttagtt gttcttatta cttgatgcag gacagctttt tcacaacctc ttttttgtat
    19561 agatgggcaa aatttttgta ataataggcg tatgtctttt tcgttgagga gatgaaaaag
    19621 catatggctt attttttgtt tgctttgctt actttctttc cacttcaagt ggcagaaata
    19681 tctacttttt attatgtaag cggaaccata tacttgaaat cagaaggaga tcatttctat
    19741 gaaaacaaga acaattgaaa tacttcacag aatcatgaca gcccaatatc cagtaaaagc
    19801 aaaacatcta gcaagtgagt tcaatgtcag tcagcggacg atccgtcagg aagttcttga
    19861 agcgaatatc tggttgagca gtaaaaagct tccggagatc cgtacaattc gcaacaaagg
    19921 ttttttgttg aagctggaaa aggaaaagca agctcgatta gaaactgaaa taaaagatgt
    19981 gaaagacggc tttttgaatc ggcgcgaacg tatttttgat ctagttttag ccgttgctta
    20041 cagccgaatc ccgatccatc taaacaaaaa agaagaagaa tttcaaatct ctaaaagtac
    20101 attagatgaa gatatgagac ggttacgagc aacactggtc agttatggga tagagatcgt
    20161 cagttatgga aagcaaggac tggtttataa aggttcagaa cgatctatcc gtacgatgat
    20221 ctatgatttt atcaatcaga atctgggaat gaccgacttc tttatgaatc aagatgcaca
    20281 attaacaccg gctcaaaaaa tctttctcca ttattttcct ttggtggaaa tcaaaaaaat
    20341 acaggagatc tattccaata agttgttgaa acaagaagat gatatttata aaaaacaaat
    20401 cctgcttttt acattgatct ggatccagcg aatcaggaaa catgaattta tctctgccat
    20461 taattggaaa aatacagaaa taaaagacag tagcttttct gcatttattg accacatgat
    20521 cgaggaattt caattggaca atgtcccgca agtagaaaga aattatcttc gtttcactat
    20581 tgagacgttc aatgctagag atatctccaa ttcaatcgag tgggtacaag ctcaattact
    20641 aacgatccaa ttgatccaat ttgtagagaa cgagacacac gtacctttcc atctaaagga
    20701 agaagcttta ttcgaaaatc tctataagca catggctgca ctgattgtaa gaatcaagaa
    20761 tcatatccaa gtcatgaatc cattgaaaga aaacatccga acaaattatt tcccaatcta
    20821 ccgcgcagtc actcattttg ttcctacgat cgaagaagta attggtggca agatactgga
    20881 ggacgagata gcttttcttg tcatccactt ttcgacgatc gccagtgtaa tcaagcgaga
    20941 cttgaattat gtttataaat cagtcgttgt ctgtaaccat gggatggcta ctggaaatct
    21001 gttagcagag aatctgaaag aaaagtttcc tcagatcgag attactgcag ttttaagttc
    21061 aaaagaaatc gaattagtag ataaattgga tgtcaatctg gttttcagca cctttcaatt
    21121 gaattatcga aataagccgc ttttagttgt cgatccgatt ttaactgaga cgaatatctt
    21181 ggttatagaa gactttttga aaagaaatga agaactacag cggttagagc ctaacagtca
    21241 tgaatcgacg gagttattca ctcaaataat agatgtcatg aaagaaagtg gtggccgagt
    21301 agatcgaatc atctatggca agttagaaaa attattcgaa acaaacaatt tagaaatcaa
    21361 caaaagggag atccagccta tgttaaaaga tattttgacg gacagcaata tcatgatcaa
    21421 agaaaaagcc actacttggg aggaatcgat tgaactagct gctagaccat tggttaaaga
    21481 aaacattgtc gaagagcgct atgtcgaagc gatgattcgt gcagtgaatg attacggacc
    21541 gtatatcgtg attggcaaac acatggcact agcccatgcc cgaccagaag atggggtcaa
    21601 taaattagga gtcagcgtag ctacgattga gcaaccaatc gatttcggta atcctgaaat
    21661 ggatccagta aagattattt tttgtttggc agcagtagac tcctactcgc atctgaacat
    21721 catgaaagaa ctaattgaat tgatcaacaa cgaagagaag ttggatcgat tgattgaatg
    21781 cacaaatatc gaagatttca aacaactgct tttttcagta gttgagaaat aataaatgat
    21841 acgggggaga acaaagatga acaaattgaa cattttattc gtatgcggtg caggtttagg
    21901 aagtagtttt gcagcacaga tgtctgctga ggatgttttg aacaaattag gagtagaagc
    21961 caaactggac cattgtgata tttcttccgc agtttccatg aaaccagatg tgatcatcac
    22021 agcagaaaat tttcgttcgc agttcgaaaa gttcgcagtt gatcctaaaa cggcgatgat
    22081 ctatttgaaa aatattgttt caaaaaatga aattgaagaa aaaatcaccc cagttctgca
    22141 agataaggga gttatctaaa tacaaaaaga aaagagggta aaaa
//
LOCUS       DACCXO010000042        22029 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.043, whole genome shotgun sequence.
ACCESSION   DACCXO010000042 DACCXO010000000
VERSION     DACCXO010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.043"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(426..626)
                     /locus_tag="IVY70_001428"
     CDS             complement(426..626)
                     /locus_tag="IVY70_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7958564.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(931..2163)
                     /locus_tag="IVY70_001429"
     CDS             complement(931..2163)
                     /locus_tag="IVY70_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP7958565.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2417..2986
                     /locus_tag="IVY70_001430"
     CDS             2417..2986
                     /locus_tag="IVY70_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7958566.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3164..3604)
                     /locus_tag="IVY70_001431"
     CDS             complement(3164..3604)
                     /locus_tag="IVY70_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP7958567.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3762..4526
                     /gene="map"
                     /locus_tag="IVY70_001432"
     CDS             3762..4526
                     /gene="map"
                     /locus_tag="IVY70_001432"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP7958568.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4558..5481
                     /locus_tag="IVY70_001433"
     CDS             4558..5481
                     /locus_tag="IVY70_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP7958569.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5557..6696
                     /locus_tag="IVY70_001434"
     CDS             5557..6696
                     /locus_tag="IVY70_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP7958570.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6689..7489
                     /locus_tag="IVY70_001435"
     CDS             6689..7489
                     /locus_tag="IVY70_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7958571.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7489..8316
                     /locus_tag="IVY70_001436"
     CDS             7489..8316
                     /locus_tag="IVY70_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7958572.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8294..9028
                     /locus_tag="IVY70_001437"
     CDS             8294..9028
                     /locus_tag="IVY70_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7958573.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            9128..9994
                     /gene="rfbA"
                     /locus_tag="IVY70_001438"
     CDS             9128..9994
                     /gene="rfbA"
                     /locus_tag="IVY70_001438"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP7958574.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            10008..10580
                     /gene="rfbC"
                     /locus_tag="IVY70_001439"
     CDS             10008..10580
                     /gene="rfbC"
                     /locus_tag="IVY70_001439"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP7958575.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10602..11630
                     /gene="rfbB"
                     /locus_tag="IVY70_001440"
     CDS             10602..11630
                     /gene="rfbB"
                     /locus_tag="IVY70_001440"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP7958576.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11728..12579
                     /gene="rfbD"
                     /locus_tag="IVY70_001441"
     CDS             11728..12579
                     /gene="rfbD"
                     /locus_tag="IVY70_001441"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP7958577.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12614..14647
                     /locus_tag="IVY70_001442"
     CDS             12614..14647
                     /locus_tag="IVY70_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958578.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14691..15971
                     /locus_tag="IVY70_001443"
     CDS             14691..15971
                     /locus_tag="IVY70_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP7958579.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            16181..16987
                     /locus_tag="IVY70_001444"
     CDS             16181..16987
                     /locus_tag="IVY70_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7958580.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16999..18219
                     /locus_tag="IVY70_001445"
     CDS             16999..18219
                     /locus_tag="IVY70_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7958581.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18209..19795
                     /locus_tag="IVY70_001446"
     CDS             18209..19795
                     /locus_tag="IVY70_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7958582.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19834..21972
                     /locus_tag="IVY70_001447"
     CDS             19834..21972
                     /locus_tag="IVY70_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7958583.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         6954 a         3726 c         4569 g         6780 t
ORIGIN      
        1 gttttcgcag caggaatttt gaaatagttc cttattagaa tagtagagat tgattaataa
       61 gctatcttcg attgctaaaa agaaaacttc tgaattccat actttataaa aatctttgat
      121 aaatctattc gaagggtcaa atttaaacca taattgtgtt tttccttcca tcagcataaa
      181 agttcacctc aaaaagagta tacgaacgaa tgttcttttt gtgaaggcgg aatttaatat
      241 actgaataat agaaaacaag tacttgtgcc aaattatgtg ccaaaaaaat tcgaattcaa
      301 caaaatgtaa acaaatagaa aaccttctat attaagattt cctgcaataa caaacacaac
      361 agaagacgtg taatagttag tcaggaacgt acaaataacc cctgtatcct ttgcggtaca
      421 gggggttatt ttgtataagt agacattgta gtttctaaac tgctatgtcc taaacgtttc
      481 gatacgctaa gtatatttac tccttgataa agtaaaatag atgcatgcgt atgtcttagt
      541 ccatcaataa acagtcgaag agaaaatagg gaaaaattga ttactaaaga aaaacttact
      601 aggtcgacgg tgtagttgat tttcatactt caatagattc gaattaaacg atttcgattg
      661 gcttatacca gtcctatcca acgaaagata tatcgttctt aactctagcg gtataacttg
      721 aagctttacg taaagccaat gggaatgaac tggagatgac tttttattat ttttagtaat
      781 agctgatggg ttaatactta caactttcct tttcaaagta ccatataaaa aaacggtggg
      841 gttgtttagc gaaataaagg atctaagcca atagcttaga tcctttattc tatgctttat
      901 attatttttt gcatgtgtgc gaaagtatac tcaagcccaa tcgccatttc ggaaaatcgg
      961 tactgtagag ccatcttcac ggattccgtc gatatccatc ttgtcagatc ctaccatgaa
     1021 atctacgtgg gtttgacttc tattcaatcc agcttctgct aattcttcat cggacatttc
     1081 tgtaccgcct ttcacactga aggcataagc agaacctagt gctaagtgat tcgatgcgtt
     1141 ttcatcaaat aatgtattga aaaagatgat gccagattga gaaataggtg aagggtcagg
     1201 tacaagtgcg acttctccta aacgacgcgc accttcatct gtatcaagca gttttgctaa
     1261 gacatcttct ccttgttcgg cagaaaaatc aacaactttt ccatctttaa aagtaaattt
     1321 catacccgag atgatggtgc cagcataact cagtggcttt gtacttgaaa tgtaaccatc
     1381 tactcgatga ctgtcaggtg ctgtgaacac ttcttccgtc ggcatatttg ccatgaattt
     1441 ttctccacga gcattatagc ttccagcgcc ttcccaaaga tggtttttag gcaatccgat
     1501 aatgatatct gtaccgggag cagtatagtg aagggcagaa aattgttctc gattcaattc
     1561 ttctgctttt tttgctaatt tttcatcatg cttcttccat gctaagacag ggtcttcttc
     1621 atagacacga gtcgttttga aaatctgatc ccataatgcc tcaacttgtt tttcttctgg
     1681 cagttctggg aagacttttg ctgcccattg tttaccagcc gcagccacga cagtccaact
     1741 gactttattt gcttgagttg ccttgcgtaa attcattagt gctttgccat tcgctgattg
     1801 ataagacgct actcgatggc tatctacacc agcaaatgca tcgggatcgg cagaaacaac
     1861 gctgatcctg cttgctcctt tttcaagcca ttcatccgct tgatcgattt tagattgtgg
     1921 tacattctca atacgatccg ttgcagcatg taaaagaaat tctctttgga tctggtcgtc
     1981 agtccattga acgataacct cagcggctcc tagtttataa gcttcttgtg tgatcaatcg
     2041 agcaaggggt gcctgttcca cgctgatctg taaaacaact gtgtgtcctt tttctgtggc
     2101 tacgccagtt tctgcaatta agcgtgcata tttttttaga agttcattaa aatcagataa
     2161 cataataaaa attcctcctc accatttttt ctggtaacta ttagcatgat accatttaat
     2221 tatgattcaa acaatgaggc tttaaataaa aaaaggaggt tattttttta aggttaagtt
     2281 tcatgatata tgttactact tgctatttca gcttgtgaag aaaatcaaaa actaattttg
     2341 catatcaaaa aaacatgtta caatgaaatt aatcaatggt ggaagcgtta taaaaaaatt
     2401 aaggagatta aaaaatatgg caagaaaaaa gacgattact agagaacaga ttttggcagc
     2461 tgcttatgaa gtagctgcca ccgatggatt tacacgattt actgcgagaa acatcgcgaa
     2521 caaaatgaat tgttccacgc aaccgattta tcttgagttt aaaaatatgg atgacttgag
     2581 acaagctctt tttgagcaga tctatgatca tttacaatat gaagtgttca ctgttgagca
     2641 tacagggaat acgattatcg atcttgcttt taattatatc cattttgcaa gaagagagaa
     2701 gaagttgtat cgttcactgt acttagaaga atatggtgga ggaaaagaga tgcagaattt
     2761 ctcttatact tactttgtga atacagtgaa acgcgatcca gcttatgtga atctttcaga
     2821 agagcagatc gaatctttgt ataatgggac atggatcata gctactggga tcgcagcttt
     2881 gatgtcttca agcatcatcc atccaactga tgaacagatt gaaaagatga tccaagacag
     2941 tatagatgct atcttgaaat tcaaagaacc aatcgaaatc gattgaatat actcaaaagt
     3001 tccacaaatc agtaggtaac aagagatgtg tcaaaataag gaaaagttcg tttttcttac
     3061 agcgtatgca tttttaactc ccgaagaact cttccatgaa tgcaatgaat aaaagagcca
     3121 tacagattga ataaatctgg atggctcttt tattgcaagt atattaaatt tttgcgacta
     3181 gttctttagc gaaagcttct aaattttcga tatcttcttc ttcagcattc agatccactt
     3241 taacattttc ggctcctttt gatgcgcctg tttttgcaaa agcagcatcg aaatcatcca
     3301 ccgatttgca gaattcatcg taaaaagtat caccagatcc gcagacaccg taaatttttc
     3361 ccaatagatc caattcttgc aagtcttcat aaaagtctac gatctcatca ggcagatcgc
     3421 catcatcgta tgtataagta gctactacgc aaatatctgc ctcctcgaat tcatctgcat
     3481 ctacttgggt acattcgttg atttcgactt cgatatccag attttcaaat gcttctgcaa
     3541 caatatcggc gatttcttca gtgtttcctg tcatgctagc atatacgatt tttgctaaag
     3601 ccatgatgtg cctcctcgtt tataattatt taacaattat gaattatacc aaaacatcat
     3661 tgaaaagaaa ataaaaccta aagacacctg gggaatgtca tttggctaaa tgtatgccgc
     3721 tatgttaaaa tgagagggat ttgtaaaaag gagacgaaaa tatgattact ttaaaatcag
     3781 cacgtgaaat tgaaatgatg gcagaatctg gagctttatt agcagatgta cataaaaatc
     3841 tgcgtgattt tataaaacca ggaatcacaa gttgggatat tgaggtattt gtcagaaact
     3901 atatcgagag tcatggcgga attgctgcgc aaattggctt tgaagggtat gaatatgcga
     3961 cttgtatcag tataaatgat gaaatttgtc atggattccc tcgtaaaaag cctttgaaaa
     4021 acggtgattt ggtaaaagtc gatatgtgta tcgatttgaa aggcggcatt tctgattctt
     4081 gttgggccta tgtagttgga gaatctacac ctgagatcga ccgattgatg gaagtaacga
     4141 agaaagcttt gtacatcggt attgagcaag cacaagtagg gaatcggatt ggtgacatcg
     4201 gtcatgctat ccaaacttat gtagaaggcg aaaatctagc gatcgttcgt gattttattg
     4261 gtcatggcgt tggtcctacg attcatgaag aaccagcagt tcctcattac ggagaagctg
     4321 gaaaagggtt gcggttgaaa gaaggcatgg ttatcacgat cgaacctatg gttaataccg
     4381 gaacttggaa aatgaaaatg gatcctaacg gctggactgc gtatacaaga gacggcggac
     4441 tttcttgcca atatgaacat acattggcta ttactaaaga tggtccaaga attttgacat
     4501 cacaaggaga agaaggcact tattgatcta tccagtcaat aggtacggga gaggtacatg
     4561 aaagtttgga acaaaataaa acaaaatgaa aatctgatgc gctttattga gacgactcaa
     4621 aagcgcatca ctgattcaga aatgagtacg acctctgtcg tcgttgctta ttatttactc
     4681 ttgtctttat ttccattgat tattgctttt ggtaatattt taccgtttct ccacatcgat
     4741 caagagactg tactgaccta tttacgagaa gtgattcctg agaccgttta ccagtttatc
     4801 ggtcctgcaa tcaaagacct tttgacacaa agttcaggag gcttgttatc cgtttctgca
     4861 ttagcggctt tatggtctgc aagtcaaagt atcaatgctt tgcaaattgc gatgaataaa
     4921 gcgtatggcg tagaaaatag aaaaaatttt ttgatcgtcc gtttcttctc actaattgtc
     4981 attgtcttgt tcatgattgc tattagtggg gtaactatcg ttcttgggtt gggacagctg
     5041 attttggatg ctattcagcc aatcgtacaa atacccgtga actttatcga tcagttccaa
     5101 actttgaagt ggccaatcac attggtagct ttatttgtca tcatgttctt gatttattta
     5161 attgtcccta atgctcagtt gaagttgaag acgatcattc cgggcacgat cttcgctact
     5221 gtcggctgga tgctgttatc acaagtgttt agtatctatg ctaggtattt tgcaacgagg
     5281 atcagcggtt atcagatcat aggaagtttt attgtactca tgctttggct taattttgct
     5341 gctacgatca tcattttggg cggaatcatc aatgcagttg ttcaagaata cgtgacgggt
     5401 catcaaatca aaaaacgtcg agatccaagc cgaaaatggt tcaataaatt gaaaactaaa
     5461 ttttctcata aaaaaaagta gaaacaagag ttcttattgt aaatttatat cagtaattta
     5521 taataattat gactcttaat taaagtaggt gcccatatgc ttgtttcttt tagtatggtt
     5581 ataagatttt ttctgacaat tttattatca ctgatattaa cacctgtatt caaaatcatt
     5641 tcagtacaaa caggtatggt cgacaaacca aatgaaagaa gaataaataa agttccaatg
     5701 ccttcagcgg gcggactgcc tatctttatt tcatttgtta tatcgacttt attcttattc
     5761 cgtaaaatta tccctcaatc gtatattgta ccgatcttga ttgcttcttt agtaatcata
     5821 gtcacaggag tgctcgatga taaatatgag ctgacaccta aacaaaaaag tatagggatc
     5881 ttgattgctt ctcttattat ttattttgta gcagatatac gtattgattc cttcacgctt
     5941 ccttttattg gatatatcca actaggctgg cttagttttc ccgtaacgat tttttggatc
     6001 ttcggtatca cgaatgcggt caatctgatt gacggtttgg atgggctggc ggcagggatt
     6061 tcattgatcg gtttaattac gataggaatc attggatatt ttttcctcca tgcttctaca
     6121 gtgtatattc cagttgttat tttctgtctt gtagcaagta ttatcggttt ttttccttac
     6181 aatttttatc cggcaaagat ttatttaggc gatacaggag ctctattcct agggtttatg
     6241 atggccgtcc tttcactaca agggctgaag aacgtgactt ttgtttcatc gatttctttg
     6301 ctgatcgtca tgggagtgcc ggtaacagac acgttttttg ctatcattag gagaaaagca
     6361 aaccgtgttt ctttttctac agcagataaa aaacatcttc atcaccgact aatcagttta
     6421 ggctttactc ataaaggtgc tgtcctgacg atttatgcga tggctttgat gttttctttc
     6481 acagctatgg tcatgaatta tactggaaga atcggaacga tcatattgat tattgccatg
     6541 ttgtttgctg caattttatt atttgaattg attggtctga tcaatgagaa acatcagttt
     6601 attttaaaaa cattgcgatt tttagggaat aaagagtacc gtacgcaagt aaaacagaaa
     6661 tatttgaaga aatggaaccg tcatatagat gaataacaaa ttaaaccagc cgatccttat
     6721 tagtattgtg acggcaaaca gtgataaaat ttttcggaca ctagataatt ttatttcaac
     6781 tgtaaaagat gaaacagcag taaaaatccg aatttttgac aacaattcac ttcccgagta
     6841 caaaaaaaga ctaacagagt ataggaaata tccctacatc gatatctatt ttcatgatga
     6901 aaattccgga tttggttatg gacataatca caatctgata gctagcgatt ctcgctatgg
     6961 cattatttgt aacccagata ttttagtgga tgaagagaca gttacagcat tggtcttgtt
     7021 attgaaagaa catccggaat gtgcgatgtt agcacctaag attcttaatg aagatggaac
     7081 aactcagcac ctcatccgtg agcagctggc tgtttttgat tacgttttgc gctttgtgcc
     7141 tttcaaatgg gtcaaaaaaa agtttgacaa acgactggct gtgtttgaat gccgaggatt
     7201 gcctactgat gctaatagtt atgtacggat gatctcaggt agttttatgg tagctgatat
     7261 tgataaattt aaagagataa aggggtttga tgagcgtttc ttcatgtact ttgaagacaa
     7321 cgacttgtgt ttgagttttg aaaaagcagg ttacaagtta ttgtacacac ccttttatgc
     7381 agttatccat ttttacggga agggtgcgca tcgaaatgca aaactgtttc gcgtgttttt
     7441 gacttctatg atgaaatttt tcaataagtg ggggtggcgt tttttctgat gaaaccagaa
     7501 atattagtag tcgttgtgct ttatcagcaa ctattcagtc aatctccagc ttatggacca
     7561 ttaagcaaag cacttagcga aaagcaaatt cagcttatta tttacgataa tagtcctcaa
     7621 aggcagcaaa atgagctgtt tgaaaaagaa aatatcgttt atcatcatga tccgcaaaat
     7681 cctggtttag cggtggctta taattttgcg ttgtctcaag cagatgaagg aacacgttgt
     7741 ctcgtcactc tggatcagga tacgaggcta gtcgacgatt attttgaact attgcgtaaa
     7801 gtaaccttta cagatgaatg tgttgctgca gttccgatga ttttttctgg gagccgacaa
     7861 gtttctcccc tatattcaga tcgttatatc aatcgtacag ctcaagcagt agaagttgaa
     7921 ggcccaacag ctgaacgaat catggcaatc aattcaggtg ttgcctggtc tgtcaaattt
     7981 ctaaaaaaga ttggcgggtt taatcctgct ttttctttag attttttgga tcattggctg
     8041 ttttggaaag tggatcagtt ggaaaaaact gtggagatcc ttcctgtacg aatggaacat
     8101 gatctttcgg ttttagacta tgaaaaagtt tcaagtaatc gataccgttc tattttacaa
     8161 gcagaaaatc gattttatca tgaatatgat caaaggcatt tatccagtca ccgacgacaa
     8221 ttattgttaa gaacagctaa acagttcttg accgtcaaaa accggcatat ctggcggatg
     8281 actttgcgct catatgttaa gaattggaag gtgtgaattc gtgcgttcgg tttgtatagc
     8341 tacctttaat ggaggcagat atttaagaga gcagttagat agtattttat ctcagctttc
     8401 agcagaagat gaagtagtca tttctgatga tgggtcagta gatgatacat gggatatatt
     8461 aactgaatac gcagaaaaag ataagcgaat ccagcttttt tcggggcctc gtcaaggatt
     8521 gatcgctaat tttggttatg cgatccagca gacgaaagga gatctaatct ttttggcaga
     8581 ccaagatgat gtttggttgg agacaaaagt gaaaaagatc actgactatt ttgagcagaa
     8641 tccggatcag ctagtagtag tttctgattt aatcattgtg gacagcgagc tgcaagagat
     8701 ccatgattcg tatttttcgt ttcgaaattc caaagaagga tggcttaata atgtgataag
     8761 aagtcactat attggtgctg gtatggcttt ccggtcttct ttaaaacccg atattttgcc
     8821 gatcccgtca gaagtgccga tgcatgatat gtggattggt ttgctgggag gaaagaagac
     8881 tgggttccta agagaaccgt taactctgta ccgaagacat gatttaaacg cgagtgagat
     8941 tgaaacacat tctaaattac ggcaaaaaat ttcttggcgt gttcatctga ttacggcgtt
     9001 gatcaaacga aaatggttta aacgttgata aaagatgttt aaatccttaa tatttccatg
     9061 acaaatataa caattgatga gataataaca aagtgaaatt tgaaatcaaa aaggagagca
     9121 gatatccatg aagggaatca ttcttgctgg gggcagcgga acacgtctgt accctttaac
     9181 aaaggcaaca tcgaaacaat tgatgccgat ttatgacaaa ccaatgattt attatccaat
     9241 gtctactctg atgttggcgg gaatcaaaga aattctgatt atttcaacgc cacaagatac
     9301 accacgtttc aaggaattat ttggaaacgg aaatgattta ggactgcaga tcgaatatgc
     9361 agtacaagaa agtccagatg gattggcaca agcgtttatc attggagaag aatttattgg
     9421 agatgatagt gtctgcttgg tattagggga caatatttac tatggtggcg gtctgtctaa
     9481 aatgttgcaa cgagcagctt caaaagatac aggcgcgaca gtatttggct atcatgtcaa
     9541 tgatccagaa cgctttggtg tagtagaatt tgatgagaat atgcaagctt tgtcgattga
     9601 agaaaagcct gctcagccaa aatcaaatta cgctgtaact ggcctttatt tttatgacaa
     9661 tgaggtaatt tctatcgcta aaggtatcaa accttcagaa cgtggagaac tagagattac
     9721 ggatgtcaat aaagcatatt tggataaagg aaaactatcc gtagaactta tgggacgcgg
     9781 atttgcttgg cttgatacag gaacacatga atcattgctt gaagcatcta cctttatcga
     9841 aacaattgaa aaacgtcaga acttaaaagt ggcttgtttg gaagaaattg cttaccgtat
     9901 gggctacatc gataaggaac aattgctggc ccttgcacag ccattgaaaa aaaatcaata
     9961 cggtcaatat ttattgaatt tggccgctga ataaggagtc cttacagatg aaagtaacag
    10021 atacaaaatt aacagatgtc aaaatcgtag aaatggacgt ctttggcgat catcgcggtt
    10081 tcttcacaga aagttattca aaagcaaaat ttgcagaaca tggattgaaa tatgactttg
    10141 ttcaagacaa ccattcacta tcagctgaag ctggtgtcct tcgggggctt catttccaaa
    10201 aaggagaagc tgcacagaca aaattgattc gggtagtcac tggagcagtt ttagatgtag
    10261 tagtggatat ccgcaaaggt agcccgactt atggtcaatg ggacggattt atcttatctg
    10321 aacataatca tcgacagttg cttgtaccta aaggatatgc ccatggattc gttactttga
    10381 caccaaatgt gaacttcctt tataagtgtg ataattacta taatgcagaa gcagatgggg
    10441 gcatcgcatt tgatgatcca gatttagcga ttgattggcc aattgatcca tcaagagcga
    10501 ttatgtcgga aaaagatcac cgtcatccga cattaaaaga atttgaagca gaaaatccat
    10561 ttgtttacgg agaaatctaa gaattaagga gaaattttct tatgaaaaac atcattgtta
    10621 ccggaggtgc cggattcatc ggctctaatt tcgttcatta cgtggtaaac aatcatccag
    10681 aagtgcatgt cactgttttg gataaactga cttatgcagg aaacaaagag aacttggccg
    10741 gacttcctag cgatcgcgta gaattggtcg taggagatgt tgcagatgca gaattagtgg
    10801 accgtctagt aaaagaagcg gacgcagttg ttcattacgc ggcagaatcc cataacgata
    10861 attcattaaa agatcctttc ccatttgtac aaacgaatat cattggtacg tatacacttt
    10921 tagaagcttg ccgcaagtat gatgtacgct atcatcacgt atctactgac gaggtttacg
    10981 gcgatcttcc attacgtgaa gacctgccag gacatgggga aggcgaagga gaaaaattca
    11041 ctgcggaaac accatataat ccttcaagtc catattcttc tacaaaagct ggttcagatc
    11101 tgttagtaaa agcatgggtg cgttcttttg gcttacgtgc aaccatttca aactgttcaa
    11161 acaactatgg tccttatcag catattgaaa aatttattcc tcgccaagta acgagtatct
    11221 taagcggtat tcgtccaaaa ttatatggcg aaggaaagaa tgttcgtgac tggatccata
    11281 cgaatgatca ttcttcagca gtgtggctga ttttgacgaa aggcagaatt ggcgaaacct
    11341 atctgatcgg tgcagacggt gaagaagata ataagaccgt gatggaattg atcttagaaa
    11401 tgatgggcca accaaaagac gcatacgacc atgtaaacga ccgcgcaggg cacgatttgc
    11461 gttatgcaat cgattcgaca aaattgcgag aagagctagg ttggcagcct gagtttacga
    11521 acttccgtga tgggttggct gacacgatca aatggtacga agaacatgaa gactggtgga
    11581 aaaaagaaaa agaggccgtt gaagcagcat atgccaaaaa tggacaataa tacagaggaa
    11641 atttcactgt attaatggaa aaacacatgt gaggcaggcg ctcgcctgtc tcttttttaa
    11701 aataaaaatc aaggagcgga taaccatatg tttttattaa ctggaggaaa tggacaatta
    11761 ggtacagaat tgcgccattt attagatgaa aaaaatctgc catatgtttc aacagatgca
    11821 aaagacttgg atatcacaga tgctgagaaa acaatggctt atattacaga attaaaaccc
    11881 gatgttattt tccattgcgc agcttacaca gcagtagata aagcagaaga agaagcaaaa
    11941 gaattagatg agaaaatcaa tgtcgatggc acaagaaatg tagcacttgc agcaaaagct
    12001 gtgaatgcac aattcgtcta tatcagtaca gactatgttt tcgatggaaa aaacaaaaat
    12061 gtagaatacg aagtagacga ccaaacgaat ccactaaatg aatatggtcg tacgaaatta
    12121 ttaggcgaaa aagcagtaga agagatttta gaagactatt atatcatacg aacttcttgg
    12181 gttttcggga tctatggaca caatttcgtc ttcacgatgc aacgcctagc ggaaacccgc
    12241 gacaaactga ctgtagtaaa tgaccagttt gggcgaccaa cttggacacg tactttggca
    12301 gaatttatgg tatatgtcat cgaagaaaaa gcaccatttg gtatttatca tttgtcgaac
    12361 gaaaatagct gttcttggta tgagttcgca aaagaaatat tgaaagatac agatgtagaa
    12421 gttgcacctg ttacgtcaga agaatttccg caaaaagcaa cacgtccgca gtattcagtc
    12481 atgagcctga aaaagacaga agcgttaggt tttgtcatac caacttggca agaagcactt
    12541 gcgcaaatgc tggaagctgc caaaaaaatg aataagtaat ttccttttta tcaaatagaa
    12601 tttggagcat gatatggaaa aacaaacaag aagtgcacga cgaaaagaaa agccaaaagc
    12661 gaatcaggca gattcgaatt tggtgcttga cctgtttcta aaactccgct atcttattgg
    12721 cgttttagtc atcgtggtca tcgttacttt taatttaaac ggaagttcac ttggtgtttg
    12781 ggataaatat gtgtcgcaaa gagacgatgg gaaaaaaact gatgtgattt ttggacagaa
    12841 tcgggaagtg cggtcagacg aatggctggt gcagacacct ttttacctct cacaagctga
    12901 agaggacttt tcgttggtga acgaggatta ttcattaaac ggacaaaaca tgatcattgc
    12961 gtataattct cctgtaaagg atattacggt tatcggaaaa ccattcaact ggggattctt
    13021 tttcctcgga agagatcgag gactttcctt ttattgggcg atgaaattag tcgtcatggt
    13081 cttgcttgga ttcgaagtac tgatgatatt aacgaagagg aaaaaagctc tatcgctaat
    13141 cggtgcattt gcgctgacat tttctccagc agtccagtgg tggttcatgc aacatgtggg
    13201 cgatttgatt ttctttactt tgggactgat gattgctttt tatcattatt tctacaatca
    13261 tgataaaaaa tggctacgaa cgttgatgat gcttttggtg gttattttcg gtattgggtt
    13321 tattttagtg atttacccag cccatcaagt gatgctggcg tacttgctga ttttttattt
    13381 tgttggcctg cttatttact atagcaagaa agtttcatgg gatctgttgg atgtagtgtt
    13441 gattggaggg gcagttttat ttattggcgg agtcatggtt catttctggc taacgtcaaa
    13501 agacgcattg ttagcttctt tgaatacctt atatccagga aaacgggtaa gtactggagg
    13561 aaagtggacg attggcgaat ttttttcttt ccttacaaat tggaaaattc cttttgaaga
    13621 tatcaactat tcgaataact ctgaagtggc cctgttctat cacttttttc caactgtatt
    13681 cctagcaagt ccatttgtgt tatttggcaa aaaagatagc gaacaaaagt tactgggacg
    13741 tattttgatg attttttgtc tgttttctat cttttggatt accgttgggt tgccaaaatg
    13801 gctagcagaa atcactctgc tgtcttatgt tcctccatta agagccatcc ttacttttag
    13861 ttttgcggct tgtttattat cgatctggtt tatcgcttat atttggagag aagatgttat
    13921 tcctatttgg tataaattca ttttgttggc agtaaatgca tggtcttatt tctatgcgat
    13981 tacgcattcg aaaatggata attatttttc ggattttgaa accatcgcag ttgctgtgtt
    14041 gggaacagtg attttagctt ttgcattatt tggctggaga cgcttgtttt acctcgcttt
    14101 tgtcgcctta gttatcgtat ctggcttttt cgtcaatcca gtcgtagaag gtacaggagc
    14161 gatttttgaa aaaacactgg caaaagaaat ccaaaaaatc gatcaacaag atcctggtaa
    14221 agtttggttg tctgaagatg aattatataa cttcacccca acattgggag tgaaatcctt
    14281 caatacagta cgcttttatc cagatatagc tgcatggaag aaaatcgatc cgaaagaaga
    14341 atacgagaag tattataatc ggtatgctca tacaagagct tttatcgcta aggaagatac
    14401 aaagttcatt ttagatcgtc cagatatgtt ttcggtcacg ttgaattttg aagacgcaga
    14461 aaaattaggg atcgactatg tgatcagtaa acgaccatta actgattaca accaattata
    14521 tgaagcgcag ttcacgcagc tatatggacc ggataaagac ggttatatga tttttaaagt
    14581 agaatatccg gattatccag atgtaccaca aacacaaact caggaaaatc agctagtcgt
    14641 acagtaatag gctagctgag aataatcctg aaatattagt agaaaggaga gtgaagataa
    14701 ttcaagcaat cgtacaaaaa tttgtcaaca aaatgagtgt cggtttcttg cttttgttgc
    14761 tgacgctagt cttttttgtt tggacagcag gggtaggttt tccagcaact ttgtttatat
    14821 ataacaatag ttcattactt atcgctttta gtgtggctat tttgctcatt ttaaatgttt
    14881 ataaagcaaa aggcgtcgat tttctttatc taggtgttgc gcttgccctt ttcattttct
    14941 tttcttactt tcgggaatta agaaatgagt ctttatttgg agacttactg tttcctttga
    15001 ttattttatt ttgtttagta attaaatgga tcgagtggac aaaatgggat cgcggtattt
    15061 atctgattgt ctttacggtc ttcctcggaa tcacgttata tcgtgtattt acagagatcc
    15121 aagtaccaga aggacaaagc atctgggctc caggcaattc gctttctgat atttggatca
    15181 atacgaatac aatcggaagt tctttgatga ctcttgcttt attgatcagt ggatttgcca
    15241 gttcttttga acgatggtat gttcgaatca tcggtgggat agcagtcgtt gcagcctttt
    15301 taggtatttg ggtctgccaa tctagaggtg cattagtcgc gttgattgtt tttgcgatat
    15361 tggatctttt acctaaatcg ttgatgcgtg tcattcgtgc gccatttatt gcctacacgg
    15421 ttacgattct tttagctttg ccgatttctt atctggcggc tgtctctgaa aaagtcaatt
    15481 tgtttaccgg tcgagaagat atttggcata aattctatca gacgctagga gaaaaatctg
    15541 aacaagtttt actaggaatg aaaacattta tcttccagcg aggaaaccaa tttttaggaa
    15601 accacaatag ttataattct attttgaata tctatggatt gataggattt gggattgctg
    15661 ctttgctgct tatccttttt attggtcgct taacgttgaa agcagattta tccaacgggc
    15721 agatgacttt tatctgggcc ttttttgcgg tgatgatgca gtcatttatg gaggatacat
    15781 tgacttcata cccatgggtt ccgattattt atatcctgtt ggctatggcc gttcatcgat
    15841 atgatgaacc agaagttacg gaggaattct atgttatgga agaggcatat gacgatatga
    15901 cttatgatga tacatcttat gaggaacaac cagatagcgc tcattcttcc aggatccaac
    15961 gctatcattg ataggcacaa aatacgagag aggctgtgac aaccgtcgca gccttttttg
    16021 gtttattgca attgtctagc atcttctgta aaatagaaac gttagagaaa atcttgtatc
    16081 atagaaaatt aagaaagaaa taagaacgca gctcggtacc aaaaatatgg taaaatggca
    16141 cttgactgta attcactgaa aatcgcttgg aggaacacaa atgtttaaag atttgtattt
    16201 atttatgaag gacatttatc agaacagaaa attactgcta cagttttcga tcaatgactt
    16261 caaatcaaga tatgcaggat cgtttttagg aatcttatgg gcgtttatca atccgctatt
    16321 tactgtattg atttattggc tggtatttgg tgttggactg aaagcacaaa tgatcgatgc
    16381 aaaataccca tttatcgtat atttagttac cgggatgatt ccctggttct tcttctcaga
    16441 tgcatttgca tcaactacgg ttgtttttcg ggaatatact tatttagtaa agaaagtcgt
    16501 attcaatatt cgtatcttgc ccacgacgaa aatcctatcc aatctgtaca cccatttatt
    16561 cttcattctg attggcatag ctgtcgcgct tataaatggt atatatcctt cagcaatgtc
    16621 tttacagctg atttattatt tattttgcat gtgtgctttc ttgacaggat tgacttggct
    16681 gacggcatcg atccaaccat ttttacctga tatcatgcaa ttaattacga tcgtgctgca
    16741 gttgatcatg tggacattac cgatattatg gagccctaat cagtttgatt caggtcttgt
    16801 aagcatattg aaattaaatc cgctgtacta tgttgttcaa ggttatcgtg agtcattttt
    16861 aagtgaagct tggttctggg agcatggaac atatactttg tatttctggg ttttcacgtt
    16921 agtaatgttc gtaatcggct ctgcagtgtt ccgtagattg aaaccacatt tttcagatgt
    16981 gctgtaagga gaatagctat gacagaatat gcaattagac taaaagatat cacaaaatct
    17041 tataatatgt acgcaaaacc ttcggatcga tttcgtgaag ccctcaatcc ttttaagaaa
    17101 tcctatcatg atgtatttta tgcgttgaaa aacgtgaatg tagaaataaa aaaaggtgag
    17161 atgatcggtt tcgtgggaga aaacggatcg ggaaaatcta caatgttgaa aatcattacg
    17221 ggagtactga caccaacatc cggcacgatg gaaatcgaag gaaaaatctc agctttgcta
    17281 gaactaggat caggattcaa tcctgagtat tctggctatg aaaatatcta tttaaatgga
    17341 atggttctag gattttcaag agaagaagtc gatgaaatgg tcgatgatat cattgaattt
    17401 gctgacatcg gcgaccatat ttatcaacct gtaaaaacct attcaagtgg gatgttcgtt
    17461 cgtctagctt ttgctgtagc gatcaatgtg aatcctgata ttttgattgt ggatgaagca
    17521 ttggcagtag gagatcttga atttcaacta aaatgtatgg aaaaatttac agaaatcaag
    17581 aactcgggaa agacgatttt attcgtttct catgatatta gttcgattcg ccgtttttgt
    17641 gatcgttcct attggttgaa aaatggaaaa gttgtcgaat atggtgatac gatggaagta
    17701 acgacgaatt acgaaaactt tttgaagaaa aaatcagtta aaacagtaga tcgaaataaa
    17761 aaccaagtag aacgcttcgc agctccagat atcgtagacg tgacgaaagc cgaattgttg
    17821 gataaagata aaaatccagt ggatatgctg gaacaaggag aaaaactata cgttaaagta
    17881 acctatgaag taaaagacga tacaatcaaa aatccagttt taggaattgc tctgcgtaca
    17941 gtcgacaatt tttatgtgtg cggactcaat actctactag atggtatcaa gatcccttgg
    18001 aaaaaaggtg agaatatctt ttatctggag tatgaaaagt taggtttgtt agctggtgaa
    18061 tattattttg acgttgcggt ctttgaagaa aatgcaacgg ttcctttggt atacaagaca
    18121 aaatacatga acttgtttgt cagcggttct tatatcggag aaggcatcgt ggtcttagac
    18181 cataaatggg aagaagggac tcatagcaat gaaatatgat tttgaaatgg acctagatga
    18241 acaaagcagt gtgggaaaaa ttgccgcaca gatcaaacca ggaagtaaag ttttagagtt
    18301 tggtcctgga aacggccgat taacgaaaca tttgatcgga gcaaaacaat gtgaagtgag
    18361 cattgtcgag ctagataagg aattgttcga ttttgtcagc gagtttgccc aagatggatt
    18421 ttatggagat atcgaaagtt ttgaatgggc aaactattat gctggacaaa catttgatta
    18481 cgttttgttt gccgatgttt tagaacattt ggtagatcct ggaaaaacgt tgaagaaagt
    18541 tagagaattt ttgaatgaag aaggagaaat tctaattaca ttccctaacc ttgcgcataa
    18601 ttctgtcatg attgatttgt tcaataatca attgccatgg gcttcttatg gtttgctaga
    18661 tgaaacccat aattcttttt atactcatga tggcttccaa aaagtttttg aaaaagcagg
    18721 attatacatc aatattgaag attatttgta cttagctgta ggtgatacag aattaaaatc
    18781 aacttatgaa gaattgccgg aagctgtacg ttatgatttc aaaatgcgac catttggcga
    18841 agtctaccaa tacttcttct cattgatgaa acatccagta gcacaaagtt ccattgcgca
    18901 gccgcaaaat tctaattatg tgaaagtatt agaagtaatc cagcaaacga aacaagatga
    18961 aataattcaa caaataccat tcaataattt cacaggggaa aatgagacac taagtttcca
    19021 tgtatcagaa ttagcagaac gaatggtgtt tcgcttcgcg cagcaaccta gctttatcga
    19081 attcagcgca gaagctgctg gagaaaaact cacatttatc gattcaaatg ctgtagtcaa
    19141 aacagtaaat gattgttatt tgtttgatgg taaagattta ccagagttct ttttaacgga
    19201 tattgcaggg aaagaagtga ctatccattg tcactatcgt tttatcggag aattgacacc
    19261 aacaatgaaa gaattgctgg aaacaatccg tccaatggct gaagttacaa agcagctttc
    19321 tgagaaaaat gatgaattag aaagagaaaa tcattatctt attgagaata atacaagatt
    19381 agatcagacg ttgaaaacaa ccataaatcg ctattgcacg ttgttggaaa gtgatgaatg
    19441 gacaatcaaa catagattag ggcgacgtaa gaaagaaaca tcgaaaaaaa tccaagaaaa
    19501 agagctttct ctatgtattg acgaaaaaat ctgggatgca gaaacaaaga tattgaaaat
    19561 catcgggtgg ggaatcgcta attcggatcg ccaaccattg tcctataagt tatcagccga
    19621 ccaatcacca ttttttgaag caattcctgt tttacgagaa gaagtcaatc aagcaaaaca
    19681 gctagctgaa ggagcaaaag caggatttga actgcggatc ctttgcgaga aggaacgttc
    19741 attcttagtt gaagcagtgg ctcaaaatgg tcaatcatgg atcattgaaa tgtaaataat
    19801 gattaactta taaagataag gagtaaacga atcgtgcaaa aaaaagaaat tgccgtattt
    19861 atcgatcaaa tcactcgtga acgggccact ggcgatttgc ttattgtggg ctgggcaatt
    19921 gatgaagtga ccaaagaaat ccctacgata aaggtagaaa aagaaaatgt gattgctgaa
    19981 gcaacacatg tcgtaagatt ggatatcaat catttatata atcttgatgt taagacccaa
    20041 agcggtttta aggtacgttt atcagggaaa atgagaggaa aagcaatctt agacttccaa
    20101 acagcaagac atcaaaatgg catagctgtg aaattaaacg gtaaatatcc ttatgatgac
    20161 ggcattgaat cttcatggga aagaaaaaaa agattactga aaaaagggat aaattatgca
    20221 aggactcatg gcgtaaaaaa agcaatccgc cgggtcaaac tggaattgaa accaggatcg
    20281 atcgactatg cagagtggat cagtcgtcat gagaaacctg atctgaaaga gcaaaggaaa
    20341 gaagtacaag gatttgccta ccgcccattg atttccatac ttatgcctgt ctataacgtt
    20401 gaaatcaaat ggctcgaaaa atgcattgat tcggtacttg atcaaacgta tgatcactgg
    20461 gaactatgta tcagtgatga tgcttctact gatccagcta tccgaaagtg tctagaatcc
    20521 tatcaagcaa aagatgatag aattaaagtc gttttccgac aagaaaatgg acacatcagt
    20581 ctggcaacta attctgcatt ggaaatggca gaaggagagt ttatagcgct cttagataat
    20641 gatgacgaat tacctccatt tgccttatat gaagtggcaa aagtgctaaa cgtacatcca
    20701 gaactggatt tgatctacag tgacgaagat aaaatcgatg cagatgggaa tcgctttgac
    20761 ccgcacttca aagcagactg gtctcccgat acattgatgg gaaataatta tatctctcac
    20821 ttaggcgtat atcggacaag cattgttaag gaacttggcg gtttccgtaa aggatatgaa
    20881 ggctctcaag attatgattt ggttcttcgt gtgacagaac aaattcctgc agaacacatt
    20941 taccacattg accgtgtctt gtatcattgg cggacgatcc cgggatcaac tgcaagcaat
    21001 ggagaagcta aaagctacat ctatgattct ggagtgaaag cattgacaga tgctctatct
    21061 agaagaaata tcaagggaag cgtacatcct ggcagaattt ctggttttta tgaaatcact
    21121 tatgatgttt tgcaagaaga cttggtcagt gtgatcattc caactaagaa tggctatgaa
    21181 gatctaaaaa cttgtgtgga ttcgatcatc gaaaaaacgt cttatccaaa ttatgaaatc
    21241 attattgcag ataatggaag tacagatcct aaaatgcaag aattatttgc agaatacaag
    21301 catcagctga aagaccgatt tatcgtcgaa ttaattgaca ttccattcaa ttattcacgt
    21361 atcaacaatc tggcagcaga aaaagcaaac ggcaaatact ttttattctt gaataacgat
    21421 acagaagtga ttgaacctga ctggatgaca gcgatggttt cttatgcaca gtttgatcga
    21481 atcggctgtg taggagcaaa attattttat ccggatgata caacgcagca tgcaggtgta
    21541 ttattaggga tcggtggcgt tgcaggacat gcattgaata actatgatcg aacacattgt
    21601 ggttactttg gccgattggt cattgacgtc aattacttag cagtcactgc agcttgtatg
    21661 atggtcaagg cagctgactt caatgctgtc aatggatttg atgaaatatt agaagtagca
    21721 ttcaacgatg ttgacttgtg cttgaaagtt tatgaactgg gtcgttacaa tgtctatgca
    21781 catcaagcag aattgtacca tttcgaatct aaatcaagag gttatgaaga tacaccagaa
    21841 aaacaaagac gttttgctgg ggaaatcaaa aaaatgcaag ataagtggcc ttcttatatt
    21901 gctcatgacc cattttataa cgataatcta actaaagaag gaatcggcga tttttcgttg
    21961 agacctgatt gattaaaaga actgtatttt catttttatt aatgaaaata cagttctttt
    22021 aatttaatt
//
LOCUS       DACCXO010000043        21737 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.044, whole genome shotgun sequence.
ACCESSION   DACCXO010000043 DACCXO010000000
VERSION     DACCXO010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.044"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(192..1091)
                     /locus_tag="IVY70_001448"
     CDS             complement(192..1091)
                     /locus_tag="IVY70_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP7958584.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1101..1457)
                     /locus_tag="IVY70_001449"
     CDS             complement(1101..1457)
                     /locus_tag="IVY70_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP7958585.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1812..2108
                     /locus_tag="IVY70_001450"
     CDS             1812..2108
                     /locus_tag="IVY70_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP7958586.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2752..3705)
                     /locus_tag="IVY70_001451"
     CDS             complement(2752..3705)
                     /locus_tag="IVY70_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7958587.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3728..4483)
                     /locus_tag="IVY70_001452"
     CDS             complement(3728..4483)
                     /locus_tag="IVY70_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7958588.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4480..5439)
                     /locus_tag="IVY70_001453"
     CDS             complement(4480..5439)
                     /locus_tag="IVY70_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP7958589.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5432..6382)
                     /locus_tag="IVY70_001454"
     CDS             complement(5432..6382)
                     /locus_tag="IVY70_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP7958590.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6532..6984
                     /locus_tag="IVY70_001455"
     CDS             6532..6984
                     /locus_tag="IVY70_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP7958591.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(7073..9340)
                     /gene="gshAB"
                     /locus_tag="IVY70_001456"
     CDS             complement(7073..9340)
                     /gene="gshAB"
                     /locus_tag="IVY70_001456"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP7958592.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9527..10525)
                     /gene="ftsY"
                     /locus_tag="IVY70_001457"
     CDS             complement(9527..10525)
                     /gene="ftsY"
                     /locus_tag="IVY70_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP7958593.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10536..11345)
                     /locus_tag="IVY70_001458"
     CDS             complement(10536..11345)
                     /locus_tag="IVY70_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7958594.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11354..14935)
                     /gene="smc"
                     /locus_tag="IVY70_001459"
     CDS             complement(11354..14935)
                     /gene="smc"
                     /locus_tag="IVY70_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP7958595.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14955..15641)
                     /locus_tag="IVY70_001460"
     CDS             complement(14955..15641)
                     /locus_tag="IVY70_001460"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP7958596.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(15989..17770)
                     /locus_tag="IVY70_001461"
     CDS             complement(15989..17770)
                     /locus_tag="IVY70_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7958597.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17796..18710)
                     /locus_tag="IVY70_001462"
     CDS             complement(17796..18710)
                     /locus_tag="IVY70_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7958598.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18737..19699)
                     /locus_tag="IVY70_001463"
     CDS             complement(18737..19699)
                     /locus_tag="IVY70_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7958599.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19699..20643)
                     /locus_tag="IVY70_001464"
     CDS             complement(19699..20643)
                     /locus_tag="IVY70_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7958600.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20644..21648)
                     /locus_tag="IVY70_001465"
     CDS             complement(20644..21648)
                     /locus_tag="IVY70_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7958601.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         6076 a         4642 c         3849 g         7170 t
ORIGIN      
        1 gtgattatcg ttcaaaaaaa gaatacacgc ttgcatctag tactctgcga gttacaaatc
       61 atccgatcaa ataaacattc gctgattatt ttccgcaaaa aaagcagcat atccacgata
      121 aatggatcta ctgctcttac ttatccttat gttttacaag gtattttctt ttacacatta
      181 ctaatgctgt ctcaatacgg aacgattgtc atcgcttgtt caaattcctc ttgcaaatgt
      241 tgtgtttgat aggcctgaaa agctgggtga tgggcacgca agaggacacg taatttcata
      301 ggatcatagc tgtctagcaa ctgttctttc aagacaaaat ctcgggtcag ctgccattca
      361 taataataac caaaaggtct gcagctgtaa atcgtgtgac cgatggattt atcttcaaag
      421 cgacgatgcg tatggccaaa aactacttgt ttgatatttg aataacggtc aagtaattct
      481 ccgaatgatt ctgatcctaa aaatgcattt aatttgttcc aacgctcata aggagcagat
      541 tgatacatga taaatgcccg tttaggaacg aaatgagtgg ataaaatcac ctcaagtttt
      601 tttgcttcca gctggtctag caaatttttt gcagctgcaa gcatttctgc attgacctcg
      661 agatctgttt tcccacgctg gatgaaccga tcataccaat acaaacgctt cattcgtacg
      721 attgcctcgt aatctttcaa ttcggaaaac tgatagtcgt accagccatt catccctaaa
      781 agaacggtct gctcatttaa agcaatatac cgttcattta aaaaatgtgg gtctggaaaa
      841 tggtccatca tctgttcatt gggcacatct gccaactcgt gattgccgaa atgaaatgtc
      901 gtagataatc ctttattgac gaaaaattcg acaacagcta ggcaacggtc tactttatta
      961 gccgtatctc cggctagatg aagccgagtg atcttgtgct tttgcaaaac ttgccaaagc
     1021 tgcatcagct ctgattcgcc gaatttattg atgtccacat gtaggtcgct gatgatcgcc
     1081 aattttccca ttttatccct ctagtctttt cgaacaactt gcaagttgcc ttttctcaag
     1141 ccgcttggtc cttttaattg taaactatta taagttggtt ctaaagcaag tgtggatgga
     1201 tcatccatgt aaagtattgc actgtctttg attttgattg gtccaactgg tgtatcagcc
     1261 actacagaat aaagacttag atctgtttgt gtatcttttt tgacaatgat caatcgaaat
     1321 gccgtatcca attggcaact gacttggctt tctgcaaatg gaccgtcacc gttttcgata
     1381 tctaataaaa gttgatcgtc tgcttctaat ttattcttca atacttcttg tgcttctttt
     1441 gtcacttcta atttcatctg tatcacctca tgtcatagaa taatcacttt attcatcaga
     1501 tgcaactaat ttgtctggtt ctgactgttc tcaatttatt ggaaagtaag tcattcgttt
     1561 attatgattt ctcttatcac aaaagagaca actttccctt ttcttcgttc agtttcttga
     1621 acggaaaaag acccaaaaag caacctgtta tttgtttaac cttattcctt tcttaaataa
     1681 caaaaaatac tataaaaaat aaaaaacctt taacaactat tggaaaaaga attacttttt
     1741 gttcacggat tattaacaac tcttcggtag agtaaactta tcaaataaaa gacttctttg
     1801 gaggaatcac tatgaaggaa tatataaaaa acatctattt tatcgaagaa acacaaaata
     1861 tcgagggttc ttatattgaa gtaaaaacac tctttgtcaa tgaagataaa accaaagcat
     1921 tggatattta taaaaaatta gcttcaaaaa aaacgaatag ttttggattg atattaagcg
     1981 agtataaaat caaagcagaa gagagttatt tctatcaatt actaaaacgg tggagtaagc
     2041 ttcccgccga tttttatcga aaaatgcaga tcatcaacta tcaaccgctt gctgagacac
     2101 atgcctaaat agaggttgtg aaatcagtgc tttgagctaa taccgaaggt cagctgattg
     2161 aacattgttt agaagagatt gtgacaactg cgatttgctt tgagtaataa gaacgctgga
     2221 cagaaaatag cttctcatat tttctagtct agtgggctta ttgccgagaa gcagcagatg
     2281 gaacatcgtt tagaagagat tgtgacaact gcgatttgtt ttgagtaata agaacgctgg
     2341 acagaaaata gcttctcata ttttctagtc tagtggactt attgccgaga agcagcagat
     2401 ggaacatcgt ttaaagaaga gatcggtaca aaataaaaat atctaaattt aagttttatc
     2461 taagtttcgc tttgtacctt tgattcaaat caaaggaggc gatcttgatg ctgaaaaaat
     2521 ggatcacact attttttgcc actccccatt ctagaatcaa cccgtatgat gtagataatg
     2581 aattcacgtt gaaaaaagat ttctcaaata aataaaaacc atccgtaaaa tcatcaatac
     2641 gattttacgg atggtttttg ataacttctt tgaggatagg ctcttgctat ttaagaatgt
     2701 aagaaacatt attgttacat agtatgagag tttaatcctc tgttatttat gctatttaaa
     2761 tgcttgattc acatcttcga tcatcagttt cattgattct aaaccgcccc cgcttaaata
     2821 ccagacatct ggttcaaggg agatgatctg ctgattttta ccagcattcg tttgtgctac
     2881 cagttcattg gcagaaatat catctttgct gtcgtctcca ccaatagctt ttgttctgtc
     2941 tactacaaat aagatatccg gatttttttc cagcacatat tcataagaaa cactttgacc
     3001 gtgagtggat gcttcgattt gatcatctgc ttgttcaaag ccaaataaat catggatgaa
     3061 accgaatcta gaaccagatc cgtaggctga taattgccct tcattcacaa gtgtgactag
     3121 tgcttttttg tcagtagctt ctgctttctt tttcgtctga tcgattgcac tagataattc
     3181 ctctaattga gttttagctt cttcttgctt accaaagatc tcccctaaag cagtgacatt
     3241 ttgctggaaa gattcccatg gatttttcgc atctaaagaa aggaaaatgg tcggagcaat
     3301 ttgacttaga tctttttgaa aatctctttg tcggccagaa atgatgatta gatctggctg
     3361 aagctgatta attttttcta agtctggttc ttttattcca cctgctgatt ctactttttt
     3421 ataggatgaa agatactcag gaaggctgct tgtagctgct ccaactacct tatctccaac
     3481 accaagagca tccatcgtat ctaaagaacc attgtcgaat acgacgactt tttctggatt
     3541 atttggcacc tcgatttggt ctccattact atctgttatc gtcaatgtag tttcagtttg
     3601 tgtgctgctt ttttttgtgt ctgcctgact ggtgttgttg ctatttccac atgctgctaa
     3661 accaaatgat gctagaatca agatacctac tactaatttt ttcatctctt ctctcctaat
     3721 gttagtttta gaaataaaga cagaatctgc ggccttcaat ctcacaaatc cgtatgtcca
     3781 tttcgtataa ttcatccaat acatcttttt ttatcatttc ttgggtcgtt ccttgcgtaa
     3841 ataactgtcc atctttcatt gcaacaattt cgtctgcata acttgcagca aagttgatat
     3901 catgaagaac aataacaatt gttttcccga tttcttgtac taaatttttt aaaatctgca
     3961 tgatttgaac cgcaaaattc atatctagat tgttcaaagg ttcatccagt aaaatataat
     4021 ccgtgtcttg cgctagcacc attgcaatat acgcacgttg gagttgaccg cctgaaagag
     4081 tgtcaatcga ttgatccgct atttcttcta acccaatgtg ttccagtgat tcctgaacga
     4141 tttcctcgtc ttttgtcgtc aattttcctt tcgtataagg aaatcgtcca aattctacta
     4201 attctttcac ggtgatttgg acatttattt cattcgattg tcgtaggatc gataattttt
     4261 tggctaattc attggatttc caagccttta cttcgttatg gtctaaatag atttcacctg
     4321 tatcttttgg tatcaaacga ctgatcatcg acaacaccgt tgactttcca gcaccatttg
     4381 gaccgataaa agcagtcaac ttcttttcag gaatcgttaa ctgtacatct ttaaccactg
     4441 attttccatg gtacagttta ttgacctttt gtaaatcaat cataggatac tcttcctttc
     4501 gcgaatcaat tttgtgacaa aataaattcc tccgacaaat tcaacgacga cactcactgt
     4561 tgtattccag ccgaaaatct gctctactgc gagctgtcct cctgtcagaa acagcatagc
     4621 taccagaaag cttccgataa atagctgacc atgacgataa gtagaaaaga cacgataact
     4681 gatatttgcg acaataaacc ctaaaaaagt aactggaccc acaagagccg ttgatgtccc
     4741 tgtcaaaaga ctgacacaac ataaaatcaa aaattcaaaa cgatctgtgt ctatcccgag
     4801 gtttactgct tgttcatttc cgagatggaa aacgtctagc acatgtcgat atcgaaataa
     4861 caggtaccag cagccgataa tgatcaaaga agcaggcaaa agatactgac tctggatatt
     4921 gccaaagctt gcaaataatc tgccttgaag caaatcatat tcattcggat ccatgatgac
     4981 ttgtaaaaag gtactgacac taccaaaaag cgtacctaaa atcatccctg acatcagtaa
     5041 taaaaaaaga ttcgctctca tttttttcaa aagaaagaaa aataacaata gactcagccc
     5101 gaccatcagc ccaacgttca ataagaaaaa aactgtacct gtctgctgaa gcatcgtcac
     5161 tcctccgata aagaagaaaa ggaccgtttg gacaaggaca tataacgact caagtcccaa
     5221 tactgaagga gtcaaaaact ggttatgcgt aattgtctga aagctgattg tggcactgct
     5281 gacagctagc gagacaagga taaatgcaaa aattttctta ctcctcaatg cacatgcaaa
     5341 atgccagttt ccatatgtat ggatcgtcaa gtacattcct gtaacaaata aaacaagaac
     5401 aagcatacca ataaaaatag atggcttttt tttaagcata tcgtcgtccc cttatcagaa
     5461 gataaaggaa gatagcccca ccgatgattc caacgatcac actgactggt atttcataag
     5521 gaaaaatcaa cacgcgagcg acaagatcgc aaaatactaa gaacaaacta cctgctaaag
     5581 cagttacgcc aattgttttg tgcacttgat ctccatatat tctagagaca agattcggaa
     5641 tgatcactcc gataaaagga atgcttccta ccatgatcaa catgactgca ctagccaatg
     5701 atactaagcc aatgcctaaa aactgcatct tctggtaatc tattcccaga tttactgcaa
     5761 acgattcgcc cagaccaatg atcgtaaatc ggtaagcaaa tagccaaacc aagagcaaca
     5821 atggaacaac agcatacaat aactcataat ttcctcgtat cacagtcgca aaattccctt
     5881 gaagccatga agtcatattt tgtaccaact ggaattgata agcaaaaaaa gtagcaactg
     5941 aaccaatgat attccctaac atgactccaa tcaacggaag catcaattga tttttctgga
     6001 ggaacaatcg cgtaagggat aaaaaaagca atgttcctgc aaaagcaaag caaaatgctg
     6061 tcaaggatcg gacaatcaag gatgcaccag gtaggaaaat catagccact aaaataccta
     6121 aacgcgcact atccatcgtg cccgcagtcg ttggtgaaac aaatttattt tgggtcaaat
     6181 gttgcatgat caacccacaa atactactgg tcgctcccac aaggatcaaa caaatcgttc
     6241 gtggcaaacg agtcactagc aagatttgta agctttgccc atctaactgg agcagttctg
     6301 acaattgtac attcgatact ccgatgaaca atgaaatact acttaaaata actaataatg
     6361 ctactagtcg tccgtagcgc atccaatcca cctttctaat tgagaatgat tttcattctt
     6421 attttaagtg aatcgttgaa caaaagcaac cgctttttca tatgttttag gtattattgg
     6481 gaatatgcta taatcgtaag aaagacaaag agaagaagga gggtattttt tatggcacaa
     6541 gatgatgtaa aaagccattt ggataaggga ctttatggta cacctttagt gaaccctgaa
     6601 gaacagcata aatatttagg gacattcagg gaacgctgct atcttagtat gactgttgct
     6661 gaaatgatcc aaccaaaaaa caaagaaaac tttttaaaag aactcaaaaa acatccagat
     6721 gcttccatac tcttgaatgg ggctctaccc ctttctgtcc aaaaccaata tatccagcta
     6781 gcaacgaaaa acaatagccg tttcactgta gtcaacgatt acgtcactgc aaatcctgag
     6841 gaattcggat tgctcttaac agcaaaagaa gcagtaaatg aacctgtgat tgatatcgaa
     6901 aaaaaatacc cagaagaaaa ctcttcgcca gctgaaaaag aaaaaccaaa aaagaaaaaa
     6961 agcttctggg atcgtttatt ttaaaacgaa aaaacacata aatttttaca tgcaaaaaaa
     7021 gcttttgatc agattgacat caaataggac aatctgtcaa aagctttttc ttttaaaata
     7081 cttcaggata taacaatttc aatacattca tcgtcaatct tctgcctttt cctgcaaaag
     7141 gataaacatg catatgcatg gctggattga aatttgcttc gatgatccca tatgcttttt
     7201 tatcagttgt aggatcgatt tctttatctg gtataatcaa atcgatacca ctaatcttag
     7261 ctcctagtgc ttctaccgca gaaacggcga tttttttgta agcttcagaa aattcatcag
     7321 tcatatcgat cgaatcacca cctgtactga tatttgagtt ctcccttaga taaacaattt
     7381 gattggcttg gggaactgat tcgatcgtca acccttgttc tttcaacatc aattgttcta
     7441 attcacctaa ttggatcaat tctaaaggtg cacgatgatt cgttccacgc aaagggtcac
     7501 tatttttttc ttcaacaagt tctttcactg ttcggatact gtcaccaata acattagcag
     7561 ggactcgcaa catgatggct tggacttctc cgtcgatcac aaagaaacga tattccgttc
     7621 ccggcataaa ttcttctact aaaaccgaac tgtcttcacg aaaagcaatg gctaaacctg
     7681 cttgataatc ttccagacta gctccttcct tgaaaatcgt gatacctaac ccgtaattgg
     7741 ttgatttagg tttgatcaca aatgcctgct cggcaaatct cggataagct tttaccgctt
     7801 cttccatgga tgaaaattct gcgcctcctg gtacccggaa cccagcttct tttaagactt
     7861 ttttcgttac tgttttgttt tccatgataa gtgggacgat atagctgtcc ttacttgtca
     7921 tgtttgcatt tttcacatat tctacatggt cttggaattg cagacgcaaa aattgatccg
     7981 actcatctaa tactttgact tggacacctt tttggatggc atcgaacata aatatctgag
     8041 tagaaagttc catttcgcga aaaccggcca actgataggg acgttcatgg ctttctttat
     8101 aatattgatg tcccaatgcc acagcaactt cttgctgact cgatagctgt gcttcttgat
     8161 agagtttcgc tgctaacgtt tgttcaggat gatccatttg agtcaatgct tcctctacta
     8221 aataaagaga atccaaccaa tccaattcaa ccagcatttg cttcattcct tcaagaattt
     8281 ctctaccttc ttgatgaaat tctgtttgat ctgatggttg ttctagtgct actttgttat
     8341 ttcgagtcgt accttctgct acccattggt cacagtcttc tttttcttct gtccataaca
     8401 tgtacatcag gaataattgt aagaaccgaa cttgctctgg attgataccc aatcttgcat
     8461 aaggattcaa atcgatattc cgcaattcaa tataacgaac acctgttgtt gctaaatctg
     8521 cgactttctt tccgcctcgg aaacgaagcg gtgtataaaa ttctttctcc tccgaaagtt
     8581 tcccttcttc gatcattcgc tcgatatctg ctaaatactg tttcatagaa gcataggaaa
     8641 ctttcacatt tggatgattc gtatagccca gcgcactgtt gcgaatactg cgaacaggtt
     8701 cttgcggatg tgattcatct aaaaagtagt tcttctcact cattggtgaa gcaccgaata
     8761 aataagtgat catccagcga tagcgcataa agtttctcgc tgttttcaaa taaagttctg
     8821 ttttgaattc ggaaaaatct tgaaattctt cctgatggct gaacaatgtt cttagcaact
     8881 catcgccaaa ttcaaaattg aaatgaatac cgcttaccat ctgttttcgc ttcccgtatt
     8941 cttttgctaa ataacgtcga tacaagacat cttcgaaatt ttttaatttt gcaataatga
     9001 tctcttcgtc tttttccggt aaggcaggtg gcatacttaa tggccagatc atctcttctt
     9061 tcggtataga acgagcagtc acatcataaa cagcagcgag atactgaaat aattcgggaa
     9121 tagaatcggt aacaggggtg atcatctctg tttgtgtttc gctaaaatct gtttggatat
     9181 aggggtgata ggatcgatcg ccaaagattg ctggatgatc ggtttttgct agatttcctg
     9241 caagatcaac ccgctgtcct tcccgttcta taccaaatcg agcttggtcg ataaaagggc
     9301 gcgcattgac atgcaataat aattgcttaa aattcatcat taagtgacct ccaattcata
     9361 tgaatctaat tataatcaaa aagtctaaga aaaagaacga aattgtttga gaaccgctta
     9421 caaataagta taaaaatgaa ggcatcaaaa acacagctat gttaaaaaca atcattcaaa
     9481 caaggagctg caacttttgt cacagctcct gctttttaga catgggttat tcttctttta
     9541 gtaatccttt gaacaatcca acgacaaaat catttggatc aaacggctcc aagtcatcga
     9601 ttccttcacc aagaccgacc aatttcactg gtaaatgcaa ttcattacgg atagcaagta
     9661 cgataccgcc ctttgctgta ccatctaatt ttgtcaaaac aagaccggtc acatctgttg
     9721 tttctttgaa ttgttttgct tgaaccatcg cgttttgacc agtggttgca tctaatacaa
     9781 gaagtgtttc ttggggtgct tctggtaatt cccgctggat tactcgtttt attttttcta
     9841 attcgttcat caagttcact ttattttgca gtcggcctgc agtatcaact agtaaaacat
     9901 ctgcctgctc tgtctttgct ctttccaaag catcgaatac gacagctgct ggatcgcctc
     9961 ctgcattgcc acgaacaact tcaacaccag cgcgttcgcc ccagacaact aactggtcga
    10021 ttgctccagc gcggaaggta tctgctgctg ccatcaatac ttttttacct tcgtttttga
    10081 actgatgggc aagtttacca atacttgttg ttttccctac accattaaca ccgacaaaca
    10141 aaataactgt tagtccgtct tcttggatat ttagttcatt tacttcattg attccttccg
    10201 cttcatagag atccacaagt ttctcgatga tcgcattttg gacttctgcc gattttttcg
    10261 catttctgag tttcacttct tgacgtaatg cttcggtgat tcgtatcgct gtttcaaaac
    10321 ctacatctgc accgataagt gtttcttcta attcttcaaa aaagtcttca tcgactgttc
    10381 gaaaattggc aaacaactca ttcatccgtt caccaaatgt tttacgcgtc ttttctaaac
    10441 ccttgtcgta cttctcctgt acttcaactt cttctgtttt ctcttcgcca gtaaaagcac
    10501 gcttgatttt atcaaaaaat cccatttgac tccccctact ctaatacaaa tttttggatc
    10561 actttggcta cgccattttc ttcattgctg gctgtcacga aatctgctgc ttctttgacg
    10621 aaaggcacag cattctccat cgctacgcct agtccagcat attggatcat cggtaaatca
    10681 ttttcctcat caccgatcgc catgatttct tcttgtttca aaccaagatc ttttgccaat
    10741 aaagaaatac cataagcttt tgtaacacct tttggcataa attccaaaag catacttctt
    10801 gttttgatga tctcatacat ttcatggaat tctgctggta tttctttgat tttaggatct
    10861 aattcttctt ggtcataagc gattactgcc ttattaagca caaattcatc tgtaatatcg
    10921 cttatatccg ctggttggaa ttgcagcagc ttattcaaca cattgtacaa ggattgcttc
    10981 cttggggcac taggcagctg taaaaccaca ttgtcagaca aaacatctag tggcagatcc
    11041 aattgctgag ccagtttgta taagcgttgg atatctgata cttccatcac tttcttttcc
    11101 atgatttcac cagtatcatt tttttgtacc aaaccgccgt tgaaagtgat gctgtagtca
    11161 ccttcatcta ccagtcctaa ttcttgcaga taatgcgcca tcgcagctaa ggggcgtcct
    11221 gtacatagaa cgatcttgac cccttgctgt tttgcttgtg ctaatgcttg tttattctca
    11281 tctgaaatca gcttttgttc atttaataat gtaccgtcca aatcgattgc tactaatttg
    11341 atcattttcg ttcctacact ttcgtttcgt tgattttcgc ctcttcattg atatcttcca
    11401 tccgtacaga aacgattttt gatacaccgg attcctgcat cgtcactccg tacagtacat
    11461 ctgctgcgac catcgttcct ttgcggtgtg tgactacgat aaattgtgtg tcatcttgga
    11521 aatcgctcaa gtaacgacca aaacgtttca cgttggcttc atctaacgct gcttctacct
    11581 catctaagat acaaaacgga acaggacaga cacggataat ggaaaagagc aacgcgatgg
    11641 ctgtaagtgc tcgttctcct ccagaaagca aacttagact ttgcagtttt ttgccaggag
    11701 gttgtacttc aatctcaata cctgttttca gcaaatcagt tggttccgtc agtactaatt
    11761 cagctttacc gccgccaaac atatttggaa agacaatttt gaattcttga cggatagctt
    11821 caaatacttc tttgaatctt gtccgcactt catcatccat ctcatccatc gtttcaaaca
    11881 attggttttt cgctgctaac agatcatctc gctgtgttgc taagaatgta tgtctttcac
    11941 tgacttgttc gtattgttcg attgaattta agtttactgg tcccagtttt tcaatctgtt
    12001 cttttaagga tgaaaccttt gttctactgt cttcgatatc ggttgtttct tgatagtctt
    12061 gggaagcttt ctcaaatgtc agctgatatt ctgactgcag ataggtcaaa tgattatcta
    12121 aaatcatttc agctcggtct ttttggactt ctaatttgga ttgttccgac agtcgtgctt
    12181 tttgctcacg attacgctct gccaagactg cttcaagctt atcgatctcc ttttgccttc
    12241 tatctcgctg ctctttggct tttgccaact cggcttttaa tgtttctcgc tgagcagata
    12301 attcgttaat ttgtttttcc aaactttctt ctgtgacttc atgatcagaa aagtctgctg
    12361 tcaatgtcgc caactgcttt tctatcgctt cttgacgttc ggttgcctct gctttttgga
    12421 ctcgggcacc gcggagctgg atctgcagat gattaaactg ctctttcaag acagcaagat
    12481 ctgcttgttc ttgtgctttt tgactttgta cttgtgctct tctggcttcc atctggtcgc
    12541 tttcttgact taacgatttg atttcttcgt cgattttttg tcgttgcgat tcaagatctg
    12601 tttgtttgtc tttcaattcg ttcgtttgct tttcgtaatc ttcgataaac tgctgcagtt
    12661 cacgtgtctc aaaagttgag atttgctttt ctttttcaaa acgttcaagt tcatttatga
    12721 tgttttgtag ctgatttgtc gcttcttgtt cttcaaaacg taattgttcg cctcgtgtac
    12781 gcaaaacttc ttgttcttcg gctaaacgag ctgtttcttg ttgcagctcc tgaacttttt
    12841 tttcttgtgc ttggagttgt ttatctgctt cttcaaattc actagtcaac tgtttcaatt
    12901 cttgattttg tacaaacaaa ctgccttggt ttcctcgttt attcgctcca cctgtcatgg
    12961 atcctccggc attcatcaca tcaccttcta aggaaaccac tcgatattga tagcggatcg
    13021 tttgcgcgat tgcattggca ctagttaagt ctttggctaa aaggatcgtt cctagcaggt
    13081 tttgaaccac agtttggatt tgatcgggat aactgacttg ttcactggca atccctataa
    13141 acccttctac agctgctgct tgtgtcaata tatgagccgg taattgtctt ggtttgattg
    13201 tcgtcagtgg aaggaatgtt gctcttcctc ctctttgctg tttcaaatac gtgatggctt
    13261 gccgagcatc tttttcattt tcaacgatga catgctgggc tgccccacct aaagctgctt
    13321 cgatagctaa cgtgaaatct gcaggaacat cgatcagttc agccactgct ccgacaatac
    13381 ccgaaagctg ctgtttatgc tgcaagacca atcgaacccc ttggtaaaaa ccaaaatagt
    13441 tttcttggat ttcttgaagg cttttcttac gagcacgtag ctgctggact tgattcatta
    13501 attgatacat tttaggctgt tcttttgcta atgcttcttg gaactttttc ccgttttgtt
    13561 ggatcatttc taacttctct tttgtttcag ttaaagatga ttttagctgc gcctgctttt
    13621 gtgtcaattc ttctttttgt aagatcaatc ggtctactga tgcttctact tccgattgct
    13681 tggctaacgt ctgcttgctt tttgctgttt cttgtatata ttgacgttct agatacttca
    13741 gttcgtttcc tacagctgct tgttcttgca tcaaatcaac atattgatct cttaattctg
    13801 ccaataattc tttcgttgat ttttggtatt tttctaattc ttgctgtgtt tttatgagat
    13861 ttgcttcagc tttttggatc tctgtttctt tttctgctgc tgctttcatc aagctttctt
    13921 gaagcttttc aaaatgttta accttttttt gtgcttctgc taatgaagtt tgatattctt
    13981 gcgagctttt ttgtgtatgc tttgtccgct cctgcaaaac atctttttgt ccttctgtct
    14041 gcttcagttt ttccgaaaga tctaataata cttgctgatt tttttcgatc agacgatcgg
    14101 cttgcgcatt ttctttacgc tgcttcgcca aaatggattc ttgttcttgg atcgactcag
    14161 atagtttgcc taattctaga ttgaatttcg caagctgtgc ttgtttgttg tcccaatctt
    14221 tttttgctgt cttgatttca gcaaccatca aagagacatc ggtttgagtc agtgtttctt
    14281 ttagccgtaa aaactccttt gctgcctcac tctgggcggc caaaggagtt aactgttctt
    14341 ccaattcgtg gatgatgtct tggacgcggc tgaggttgtc ttctgtttca aaaagcttct
    14401 gttctgcttt ctttttccgc tgcttgtatt tcaagacacc agctgcttct tcaaagatcc
    14461 cacgacggtc ttccggttta ctactgaaaa tcgcttctac ttttccttga gaaatgattg
    14521 agaacgattc ttttcctaat ccagaatcta agaacaattc ttggatatct tttaatctgc
    14581 agctttgttt attgatgaaa aactcgcttt ccccagtccg tcgataacgg cgggttacac
    14641 tgatctcatt gtactccaat ggcagataat gatcactatt gtctaaaatc actgtgactt
    14701 ctgcaatatt cagctgctta cgggtatcgg aaccggcaaa gatgatatcc ggcatttttc
    14761 cgcctcgcag acttttagca gattgttctc ctaataccca gcggatagcc tctgtaatat
    14821 tgctttttcc gctgccattt ggtccaacca ctgctgtgac gctattttca aaatcaatga
    14881 ctgtcttgtc tgcaaatgat ttaaaaccgg ctatttcaat tcgtttcaaa tacaaagctt
    14941 tccctcactt acttttacag tgctgccaat gcattttccg ccgcagcttg ttctgctaat
    15001 tttttcgatt tcccttgtcc agtgccaatc aattgatcat ctacataaac ttcgatccaa
    15061 aatactcgtt catgagctgg accttcttca ttgatcaatc ggtattcgat tgatacatca
    15121 cctgattttt gcaagacttc ttgtagtttt gttttatgat ccatctcatg tgaaaaagca
    15181 cctgctttga ctttaggaaa aatcactttt tcgatgaatg catgggtcgt gtcgaatccc
    15241 tggtcaagat ataacgcgcc taaaaaagct tcgaataggt cgcaaagcaa tgctggtcgc
    15301 gtacgtcctc ctgaattctc ttctcccttg cccaacaaaa catattgatc aaagtgacat
    15361 tctttggcaa atttcgacaa gctgtcttcg cgaacgatcg cagctcgtgt ttttgtcaat
    15421 tttccttctg gcaaatgagg atataaacgg aataaatact gagaaaccat cagttctaaa
    15481 acggcatctc ctaaaaattc aagtcgctca ttatccgata attgcagatt gcgatgctca
    15541 ttcacatagg atgaatgggt aaaagcttgt tcaagcaaat ttagatcgtg aaaaacgatg
    15601 ttatagtttt cttttagttc ttttgtcagc tggttgtcca ttttccacat tccttttatg
    15661 gatatttttt cgatacttcc ttattatact ggttttttgt caaatttgaa atggttcggt
    15721 caaaaggtaa gtaaagcttt aataatccta gtgattgacg agaagttgtg aaaaattcgt
    15781 ttagtttcga gtatgaagaa aaaattttgt gaaaatggct ttccatattt ttgcataaat
    15841 ttgacttaat accgagaaac tggactttga acaccgttta ttggagaagt catgaaatca
    15901 gcgccttgac ctgaatatta gagaaataaa cagaaaaata tcaaaaaacc gaaaaaggac
    15961 atcgcccttt ttcggtcatt tttagtgatt atgaacgtga atctgctgta acagaaacat
    16021 ttgcccatgg gtttggtaca tcataagacc aatcccagtc tttgacacgg tcgttgactg
    16081 gcaatacttc attacggaac aacgttggaa tcgcaaatgc ttcttcaaat gcatattgtt
    16141 gccatttttg atatgcttct ttttggtact cttcatcaaa tgctttgctt gaactgatat
    16201 cgttgatcaa ttttgtattt tcttctgatt cataacgagt gtagttgaac gcagcatttg
    16261 gtccccatag tccacttgga tttggatcag ttcctgtatt ccaagcagct tggtaaacat
    16321 caatttcagg atcatcgttt tctaatttat catagaaaga attgaattcg atcagacgac
    16381 ctgttgtgta ttggacatcc aaaccaattt ctttccattg ttggatatag taatctgcca
    16441 atggttgcgc tgtttcccca ccggacatcg aagcaaagtt gattgttagt ttatttccat
    16501 ctttgtcttc tcttaaacca tcgtcatcca catctttgta gccagcatca tctaatagtt
    16561 ttttcgcttt gtctagatct tgtgtgaatc ctttcatctc atcatcatgg tatgactgga
    16621 atactggagg aatcaatgaa gtcgcattgc ttctcaaacc tgcatagaaa cgttcaccta
    16681 ctgcattatt atctaatgca tagcccattg cttgacgtaa tgatttatca gccatttttg
    16741 aatctggatt gtattccaca ctctttgtat ctgcattcca tttacctaac ttgaatccta
    16801 gataagtata agaagtttgt tcacggccta agtttgtata gccagcaatt tctttatagg
    16861 tatcgtaagt atctgttggc atttggaaaa tcatatcata taatttagag ttcaatgctt
    16921 cagttgcact tgccatcggt acagatttca tcgtgatctt tttcaattta ggcgtaccat
    16981 tccagtagtg ctcgtttggc aagtattcta ctgattctcc tgcaacgata ttactcatgt
    17041 aataagggcc aaaagtcact ggatttttac gtacttgatc gcttgattcc atatctttga
    17101 ttgctacacc ttctaacgta tgttttggtg ctgcatatga ccagatacca ccacccgatt
    17161 gaagcatgct tgggtttact tctttatatt cgatttcaac tgttttgtcg tctatttttt
    17221 tgattccgga gattgtgtcg gcacttccat catgatattc ttccatacct acgatgttac
    17281 ggaaagtgtc gtcataacgg ataccagtgt aatctttatt cccaatgatt tcgtaaggga
    17341 aaatcacatc gtccgccgtt acatcttcac catctgacca tttgacgttg tcttttactt
    17401 tgattgtcgc ttttttcgta tcttcatcta gatcaagcgt tgcagctccg tcgtctgtga
    17461 ttctgaaatc gtcatcgttt gcaaatagcg gttcatgaga aggagccata aaagctgcat
    17521 catatgaatc ttggtaaaac tcccactgga acaatccttt aaactgactg tccgttgcga
    17581 ccgcagcttc taattcgccg tcttcgattg catcatcatt attttttgtt gcaattggaa
    17641 aagttaattc ctctttgtta ttgccgttgc ctccagagtt gtttccagag gtatctgtac
    17701 ctccgccccc gcatgctgca agaacgacag cagacatcag cgctgcaaat ccaaaaatcg
    17761 ttttcttcat tcctatttcc cccttcgctt ctatactagc ctaaccgttg gcgcgcatct
    17821 gcagaacgct tgaatgcttg tccaacataa ttgatactca acatcaaaac taaaataagg
    17881 attgatgcgg gtaaccagat ccattgccga ttcactaaga cttctccgtt acttgcataa
    17941 ccgatcaatg tacctaaact aggtacattt gccggcaagc cgaaacctaa gaaagtcaaa
    18001 gttgtctcga taccgatgtt tgccgcaaag ttcatcgtca aattagtaat gattaatgaa
    18061 cttagattcg gcatgatttc gcggaacatg attttaaaat cattggttcc cattgttttt
    18121 gaggcactga cataatctct tcttgcttca gataatgtct tgcttcggaa taatcgggct
    18181 ttagctaccc aataaaatgc actcatgatg aatataaagg accagacatt gtatctggaa
    18241 ataatcgata cgaagacgat aatgatcagc atgatcggta gaatcatgat aaaatcaacg
    18301 atccgcatca agatgttgtc aatgatccct ccgtagtagc cagagatgat ccctaatcca
    18361 accccgatga tcgaagtaag gatcgtgatc gcaaatccga tcgtaacaga gtttctagca
    18421 cctatgatca attgtcctaa tacatcacgt ccgccttcat ctgctcccag aatgaacttt
    18481 tgtccttgtg tcgaaactgt tcctggtgca gcgtatttgt cgaagatact aacatacatc
    18541 actttgtctt gatcggtcag catcgcaccg ataaagatca ctaataataa aaccactagc
    18601 aaacctaatg aaaaaatagc aattttatct tttttaaatt ctcgagcaat cattcgtatc
    18661 cccataggag gaatactttc ttgaactgct gtattgtttt tttcactcat cgatccattc
    18721 tcctttctga ttggttttat tgaatccgaa tacgcgggtc aacgatactc atgatgatat
    18781 ctgaaagtaa tgtccccact agcgtcgcga ttcctaaaat caatactaag gatgtgatca
    18841 cggtatagtc ccgttggccg atggaatcaa taaatagttt tccgactccc ggataaccaa
    18901 aaatcttttc gataactaca gaaccagcaa tcaacgaagt gatttcatac cccatttgag
    18961 aagccatcgg caacgctgca tttcggaaaa tatgtcgact gaatacttta ttcgtcggta
    19021 cccctttgga acgggctgtt ctgacaaaat cgagcgattt cgaatcgatt acttcacttc
    19081 gcagatattg gattgtgacg gcagtcgcca aaaatgcttg ggtaattgat ggtaacagaa
    19141 tatatttcaa acggtctatc cattgagtga taaatccacc ttgcaaacct agtgtttgtg
    19201 atccacttgt tgggaaccag cctaaacgat aaccaaaaac aaatagcatg atcaagccga
    19261 agataaataa aggaacagca aaactaaaga agttgtagat tacgacaatc ttatccaaga
    19321 ttgaattttg ataccggcca gcaagcatcc ccagaggcac agcaatcaaa taagtaagga
    19381 caactgtcaa gatcgataga tataaagtgt tgatgatccg ttcgccgatc aagtcagcaa
    19441 cagagcgttt gtataagaaa ctttgaccaa aatcgccttg caaagcattt ccgatccaac
    19501 ggaagtactg gacataccat gggtcattca agccagctgc ttgtcgcatc tgttcgatca
    19561 ctgccggatc ctgatttgga ttgatcaaac cggtaaatgg atcccccggc atcgcttggg
    19621 ctaataagaa gatcagaaca cttaaaataa taacttgcgg gatcatcaat aaaacacgtc
    19681 gcagaatcgt tttccacatt agcttgcacc tccatctttc aacgccactt tgtgtgtagt
    19741 tgtgatcgta cgcaaatcgt agactcgacc agtcgcatca tagtattctt tttggttttg
    19801 gatatactct tgttctactc ttcgtcgatt ttctttgtgg ctttccctat tttctacatc
    19861 gatttgcgga atagctgaaa gcagacgttt cgtatagata tgtcgcgggt cattataaat
    19921 gtcttctctt gtaccgattt ctacaaatcg acctttgtac ataattgcga tattgtcgca
    19981 catgtgtttt actacaccta aatcatgtga aatgaataaa tagctgaggc cgtattcttg
    20041 ctggattcgt ttcatgaaat tcaatacttg cgcttgaaca gatagatcca gagcagatac
    20101 aggttcatcg gctacgatca gttttggatt ggtagcaaca gcacgggcta ctcctagacg
    20161 ttgacgttgt ccgcctgaaa attcatgagg atatttatat agcgcatctt ccggcatccc
    20221 aacgatatct agcagtcctt ttaccttttt cttttcttct tgatcgctta accgttcaaa
    20281 atttcggatg ggttcagcaa tgatatccaa cactcgcttt ttaggattca aactggacat
    20341 tgaatcttgg aagatcatct gcacatcctt gttataaccc atttttttgc ggttgcttcg
    20401 cttcgttaca tcttttcctt catacatgat ttgtccggct gttacctttt ctaatcccac
    20461 aacggctttt cccgtagtag attttccaga gccagactct cccactaaac catatgtctt
    20521 tcctttttcg ataataaaat ccacaccatc tacagcatat acatgatccg ttactcgatt
    20581 gaagaatcca ctgcgaatag ggtaatgaac tttcaggtct ttgattgtga ttaattctgt
    20641 catctacacc tctccttttt cgtctctaaa gtggaaatgc ttatagcagg agcaacgtac
    20701 caagtggttc ggtgcgactt catgaaggat aggctcttct tcatgatcgc ttgctggtat
    20761 ccatggaata cgtggtgcaa accgacaacc tttacgaggc atgtttttca gagaaggaac
    20821 gacaccttcg atcacatgga gttctgcatc atgatttcct tcttgtggta tagagttcag
    20881 taaagagcgt gtataaggat gcttaggatg atcaaacagt tcttttacac ctgctacttc
    20941 aacgaactgt ccgccataca tcactgccac tcgatcagcc atttctgcta cgactcctaa
    21001 gtcatgagtg atcaagatga tccctgtttt tgtttcttcc tgaaggtctt tcagtaaatc
    21061 aagaatctgt gcttggatgg ttacatctag ggctgtcgtc ggttcatctg caatgacgat
    21121 tggaggtttg caggcgattg cgattgcgat gatgacacgt tgtctcatac cgcctgatag
    21181 ttcatgggga tattggcgtc caactctcgc aggattagga atccctactt gttctagcaa
    21241 ttctagtgca cgagcttgac gctgttctgc attcatcttt gtgtgatagg aaagtccttc
    21301 tttgatctgg tcttcgattc tcatgagagg atttaatgca gacaacggat cttggaaaat
    21361 cattccgata tcatttccgc ggattttatt atagagcgtt tcgttcaggt cgattaaatt
    21421 cagatcgtta tataaaatct cacctgtaat ttttgtgtta ttaggatcaa gtaatcccat
    21481 aatcgtagta gccaaggtac ttttcccgca cccagattcc ccaacgattg ctaaaatctc
    21541 gtttttgtct aaagtaaatg aaacgtcatc tactgcatcg taatagtcat ctttgagtcg
    21601 gaatcctgta tgtaggttct gaacttctaa taattggtta tcggacaatc gaatacccct
    21661 ctctgcttcc taaatggaag tttttcttaa taaaaaagga cattacacag aataaatcgt
    21721 tttataacaa aggttat
//
LOCUS       DACCXO010000044        20678 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.045, whole genome shotgun sequence.
ACCESSION   DACCXO010000044 DACCXO010000000
VERSION     DACCXO010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.045"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            352..1041
                     /locus_tag="IVY70_001466"
     CDS             352..1041
                     /locus_tag="IVY70_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7958602.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="IVY70_001467"
     CDS             1028..2236
                     /locus_tag="IVY70_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7958603.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="IVY70_001468"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="IVY70_001468"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP7958604.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="IVY70_001469"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="IVY70_001469"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP7958605.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="IVY70_001470"
     CDS             complement(6339..7034)
                     /locus_tag="IVY70_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP7958606.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="IVY70_001471"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="IVY70_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP7958607.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="IVY70_001472"
     CDS             complement(8233..8424)
                     /locus_tag="IVY70_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP7958608.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="IVY70_001473"
     CDS             complement(8445..9335)
                     /locus_tag="IVY70_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP7958609.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="IVY70_001474"
     CDS             complement(9322..10089)
                     /locus_tag="IVY70_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7958610.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="IVY70_001475"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="IVY70_001475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP7958611.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="IVY70_001476"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="IVY70_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP7958612.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="IVY70_001477"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="IVY70_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP7958613.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14705..15043
                     /locus_tag="IVY70_001478"
     CDS             14705..15043
                     /locus_tag="IVY70_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958614.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15211..15969)
                     /locus_tag="IVY70_001479"
     CDS             complement(15211..15969)
                     /locus_tag="IVY70_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP7958615.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="IVY70_001480"
     CDS             complement(15987..16805)
                     /locus_tag="IVY70_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP7958616.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="IVY70_001481"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="IVY70_001481"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP7958617.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="IVY70_001482"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="IVY70_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP7958618.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="IVY70_001483"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="IVY70_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP7958619.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="IVY70_001484"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="IVY70_001484"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP7958620.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5924 a         4218 c         3797 g         6739 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20641 aaaaataaaa ttttaaacag aaggccctaa aatctctt
//
LOCUS       DACCXO010000045        19211 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.046, whole genome shotgun sequence.
ACCESSION   DACCXO010000045 DACCXO010000000
VERSION     DACCXO010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.046"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            299..1960
                     /locus_tag="IVY70_001485"
     CDS             299..1960
                     /locus_tag="IVY70_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7958621.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="IVY70_001486"
     CDS             complement(2030..2377)
                     /locus_tag="IVY70_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP7958622.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /locus_tag="IVY70_001487"
     CDS             2461..3402
                     /locus_tag="IVY70_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7958623.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="IVY70_001488"
     CDS             3403..4446
                     /locus_tag="IVY70_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7958624.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="IVY70_001489"
     CDS             4452..5522
                     /locus_tag="IVY70_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7958625.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="IVY70_001490"
     CDS             5512..6465
                     /locus_tag="IVY70_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7958626.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRYDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="IVY70_001491"
     CDS             6477..7487
                     /locus_tag="IVY70_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7958627.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="IVY70_001492"
     CDS             complement(7772..8593)
                     /locus_tag="IVY70_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP7958628.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="IVY70_001493"
     CDS             complement(8690..8968)
                     /locus_tag="IVY70_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958629.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="IVY70_001494"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="IVY70_001494"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP7958630.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="IVY70_001495"
     CDS             complement(11724..12311)
                     /locus_tag="IVY70_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958631.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="IVY70_001496"
     CDS             12486..13808
                     /locus_tag="IVY70_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7958632.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLTLIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="IVY70_001497"
     CDS             13963..14739
                     /locus_tag="IVY70_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP7958633.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="IVY70_001498"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="IVY70_001498"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP7958634.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="IVY70_001499"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="IVY70_001499"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP7958635.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="IVY70_001500"
     CDS             16629..17258
                     /locus_tag="IVY70_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958636.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="IVY70_001501"
     CDS             complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="IVY70_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP7958637.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18700..19122
                     /gene="mgsA"
                     /locus_tag="IVY70_001502"
     CDS             18700..19122
                     /gene="mgsA"
                     /locus_tag="IVY70_001502"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP7958638.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
BASE COUNT         6282 a         3456 c         3913 g         5560 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgaggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggtatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta acattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    17281 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    17341 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
    17401 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
    17461 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
    17521 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
    17581 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
    17641 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
    17701 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
    17761 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
    17821 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
    17881 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
    17941 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
    18001 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
    18061 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
    18121 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
    18181 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
    18241 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
    18301 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
    18361 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
    18421 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
    18481 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
    18541 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
    18601 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
    18661 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
    18721 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
    18781 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
    18841 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
    18901 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
    18961 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
    19021 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
    19081 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
    19141 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
    19201 atataatacg a
//
LOCUS       DACCXO010000046        18459 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.047, whole genome shotgun sequence.
ACCESSION   DACCXO010000046 DACCXO010000000
VERSION     DACCXO010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.047"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(61..1696)
                     /locus_tag="IVY70_001503"
                     /pseudo
     CDS             complement(61..1696)
                     /locus_tag="IVY70_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2251..2814)
                     /locus_tag="IVY70_001504"
     CDS             complement(2251..2814)
                     /locus_tag="IVY70_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP7958639.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(2829..3872)
                     /locus_tag="IVY70_001505"
     CDS             complement(2829..3872)
                     /locus_tag="IVY70_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958640.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(4005..4409)
                     /locus_tag="IVY70_001506"
     CDS             complement(4005..4409)
                     /locus_tag="IVY70_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP7958641.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(4642..5277)
                     /locus_tag="IVY70_001507"
     CDS             complement(4642..5277)
                     /locus_tag="IVY70_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP7958642.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(5543..6595)
                     /locus_tag="IVY70_001508"
     CDS             complement(5543..6595)
                     /locus_tag="IVY70_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7958643.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(6608..7546)
                     /locus_tag="IVY70_001509"
     CDS             complement(6608..7546)
                     /locus_tag="IVY70_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP7958644.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(7997..8647)
                     /locus_tag="IVY70_001510"
     CDS             complement(7997..8647)
                     /locus_tag="IVY70_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958645.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(8747..9256)
                     /locus_tag="IVY70_001511"
     CDS             complement(8747..9256)
                     /locus_tag="IVY70_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP7958646.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(9457..10326)
                     /locus_tag="IVY70_001512"
     CDS             complement(9457..10326)
                     /locus_tag="IVY70_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7958647.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(10346..11467)
                     /locus_tag="IVY70_001513"
     CDS             complement(10346..11467)
                     /locus_tag="IVY70_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP7958648.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(11469..11864)
                     /locus_tag="IVY70_001514"
     CDS             complement(11469..11864)
                     /locus_tag="IVY70_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7958649.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(11883..12680)
                     /locus_tag="IVY70_001515"
     CDS             complement(11883..12680)
                     /locus_tag="IVY70_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7958650.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(12682..13470)
                     /locus_tag="IVY70_001516"
     CDS             complement(12682..13470)
                     /locus_tag="IVY70_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7958651.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(13484..13960)
                     /locus_tag="IVY70_001517"
     CDS             complement(13484..13960)
                     /locus_tag="IVY70_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7958652.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(14032..16689)
                     /locus_tag="IVY70_001518"
     CDS             complement(14032..16689)
                     /locus_tag="IVY70_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7958653.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKR
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(16708..17112)
                     /locus_tag="IVY70_001519"
     CDS             complement(16708..17112)
                     /locus_tag="IVY70_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HAP7958654.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(17344..17601)
                     /locus_tag="IVY70_001520"
     CDS             complement(17344..17601)
                     /locus_tag="IVY70_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAP7958655.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(17726..18058)
                     /locus_tag="IVY70_001521"
     CDS             complement(17726..18058)
                     /locus_tag="IVY70_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958656.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT         5850 a         3721 c         2711 g         6177 t
ORIGIN      
        1 atttggttta ttacttactt tttcttattc tctagtaagc taatattgaa aaaaatgtaa
       61 tcagatcatt atttttatat gttgtaactt cctgataaga aaactaatta aaagaaagag
      121 gtaactacat aaactaatta aaaaagctaa cttcatagtt ggaggataaa tggaagaaac
      181 ttcaatgatt gtttctcctt gtgttccttc aatcgacact agtcctatta tcaatctctc
      241 ttttttgtcc atttactttt gcgactgttc cccaataatc aaatacagga agcgttattt
      301 tttgtccttt agattcatgt actgtataga ctattttcga atccatcact tgataaggca
      361 tagtatgcca ttccttgcca attcttactt catgattctg caaatgttct ttgtattgta
      421 atgcttccgt gggtgcatag tcataaagtc ctccgggaag gagtgtcgtg taacgaataa
      481 gattttcttg attcattacc aagtgatctc tctgagtgac aactttatgt tcatagtttg
      541 cttttgttgc agcagataca gccaacagta ataaaacaat tgccgttata ttaactactt
      601 ttctttgctt agctgatgtg ctcttcatga aaacgaacga taaaatcaac gagccaaaca
      661 gtacttgaaa gcctaagatt cgccaaggaa attgaatcat ttgaatgggt gtattttgta
      721 ataaagacca tggaaaaaat ttggtggata agagccaaga gactaccgca ctaaggtaaa
      781 tcactttttc tttgcgatta aatcctttaa aaaagaagag tggacagatc aaagaaataa
      841 taccaatgat tcctaccgta aaggttgtca ggtcattatt catcgcacca atcaaactat
      901 ccgagacatt taatgcttgg tgttgtaagg tcgttttatc tggctcatta atcgattgaa
      961 ccatgatttg tcggaacatc ggataccaaa agtatgctgt taaaagaatc gtcattccta
     1021 ctgcctttga caactccaca atcaaccgga tgctttcttt ttttcttgtt tcacttacta
     1081 caaagccgcc taagactagt aagccaacgg ctattacact cataaaagtg gaaagaacat
     1141 gtgtataaat gagtaagctc atactgactg ctaatggaat tgcacatggc tttttctcat
     1201 aaatcagtgc gtaagcataa tacataacta tcggtataac cgtaagtgca atcgcttcag
     1261 caatagcaga acgatgataa atatcaatcg tacggtaccc ataaaatgta tataaacagc
     1321 taaatataaa agccgcatcg attcttttta aaaattttag tccataatga aacatcagca
     1381 tgatagtaca aacattcaat agccaaacat ataagatata actaacgatt aaattattac
     1441 ttatccaata aaaaatgact gccggaaaaa aggtcaagta gggataaaaa taattcactc
     1501 cgctcccata atgattaaac gtcgtaaaat tgattggacc agaaaaaatt gaagataacc
     1561 ctttgactct agcaaaatgg aaggcaatat cccccgatgt agaaatagta tgaacatttc
     1621 taaaataatt gattgaataa agtccagcaa taattgctag gagaatggac aatattattt
     1681 ttttctttac ttgcattact taattatata ctcctttcat ggattgcttt atctagtata
     1741 acttaaattt taaataaatc gttatttatt tttactctca cttaaaaaag cactaaaatt
     1801 ataagagagg gaggtaatgg tctgaacacc aagaattgtt ccaacatcga attgcttaac
     1861 taatgagttc aaagcttaat tatgtggctc ttatatttac cgagcgtgga ttgcgatctc
     1921 tttagcgaaa caaacatctt tcataaaaaa aacagccata tccgctttct tggatatgac
     1981 tatttactac tactttacta tctttcattc ttcttgtctc acttatgata aggcgctgcg
     2041 tatcatgagt gaatgatatt aggaaaactt cataaaataa atcttttata catttccact
     2101 tctagatggt cattcatgtt agaagaatca aactattttt ttactgtcgt aataatatta
     2161 aaacactaga acaaatcaat taaattccat aaaatataaa taatagacct agcatcatct
     2221 ggtataaaat ggatgctagg tccgttatat ttactcaaaa ttgattctac gaatagatct
     2281 tcttatcatt tctattttag tagtcatgcg ttgttgttct tctaaaggtg gtaatggtat
     2341 aagtagttta gataaatttg taccatttac atttggttgc ccagttccgt aagaaatttt
     2401 ttctaataac ttccaatata atggcgagtc taagaagcag tctacgtatt ctgataataa
     2461 tttctcaact aacctaattc gaatcaagta agaagcaaaa acagattcct cacttatttc
     2521 cttaaccaag aaactttttc ccatagttcc tcctgtcctt gctatcaaaa tgtcattttc
     2581 ttccaatctt aaatcaacta gtttagaatt actaatatca cagtaaggca cactggacca
     2641 atttacacgt ccctcttgta tatctgtgat tctcaaaaac ttaacattgc cttgtttttt
     2701 cgccgaatct gtatagccat attgaatctt agtagaaata cttcctaaat aaacccatag
     2761 tcgaacatcg ctagatgatt tttcttgtcg ttctttatga acaacagtga tcatcttgag
     2821 ttgtccgact attttttagt tgcagcaatc tttttgggac gcttatttgc atgaagcaac
     2881 gttgctaagt ctagtaataa aacattggct aacacaggat tggctttctc gcgatacgct
     2941 gcggcgccac tttcaaacaa tactgctaga cgatcttcta caacaacgat ttgttccaaa
     3001 tacggtggaa atttgattcg ttttaggatc cggctaggag agtcccgctc ggacactaag
     3061 agttccgagg tataaggacc ataagaacga ctggctaaga gataatggcc ataaaaagcc
     3121 acaccttgaa tcttatcaga tgtttttctg ctcattgtca cggttggttg gattttgtct
     3181 ttgcggcgaa ttgctgtttt catttcagct atcgtcggca atagatagaa agtcaactgt
     3241 ccttcacctt ttagtgtgaa gttgccagat accagttgat cattgttttg agtcaaatac
     3301 gatgcttgtg gaatctctga gagatcagtt gtcagccaat aagcgattgg ctgtcccgtc
     3361 tcttcagatg tatctgctaa gatatcgatc aaacgtaaag cggctactcg tccagtagca
     3421 gatcctgtga ttgtcaacca taaaagttcc cgttttgtat catatactaa gccccctaca
     3481 tgtggccgtt taggtaaaat cagcagtttg atcggctgtc ctgtttctct atccagtaca
     3541 tgaataatag aatgatgctg atggtcatag cagtaggcag aaataaacac aaaatcttga
     3601 gaaaaagcca acccttgcgg cgtcatccat tgacaaacct cattttcgcc ggtttccggc
     3661 accaccattc ttgtttctgt taatccagga atgttatatt gttttgcttc agccttttcc
     3721 caagctgctt gaacaatagg ataccgtttt aacttttttt caatatcctg atgtatccgt
     3781 tgctgcttcc aatgcttaaa ctttaggaca tagtaaacaa gaaggccaat gaccagcaac
     3841 actaaaatca ttattgctac ccgctgtgtc atttttttgc ctccttgttc tattcgatca
     3901 atcgtccata taccgctatt aattaccgta actacatgtt tcttttacta gcgtttcttt
     3961 ataggatctt tcataatctc atttatagta acattaagaa tcccttattc tactatagtc
     4021 acttccacat caatattgcc tcttgttgct ttagagtatg ggcaaaatgc gtgggcttct
     4081 tctgcgattt tctctgcttg gtccttctca agtcctggca aagaaacttc taaaacgacc
     4141 ccgatttggt agcccccatc ttctaattga aataaagata cccgtgcttt gacttcagaa
     4201 tcagactcaa gattattttc ttccagcatg tgttgaaccg cgccgttgaa gcaagatgcg
     4261 tatccagccg caaaaagttg ttccggattt gtcccttcac tatggattcc tgggcggtcg
     4321 atcttcatgt gcatacttcc gtttggtgcc tcaacttcgc catcacggcc tccacgattg
     4381 atcattgctg tttcgtacat ttttttcatt gagatcatcc tttctttcgt cgttctatat
     4441 taagtgtaac gagtattttt taatctgaca aaggtaatgc ttgcattcgg cgacatcagt
     4501 tagacaacat cgatgatcag tgatagaagc agtttatcta tacaaaaaaa gaagggttct
     4561 atgatatttg gtgtccttac tcgccttgag taacaacacc aaatatcata gaaccactta
     4621 atcagcttca cactgctttt tctactttcg tagttgaaaa aaacttaaac aaatcatcgg
     4681 caaatagtta aagaaaaaac ttaatcgttg aaagaagccc aattgcttaa agaaggcaaa
     4741 ctgactaatc gacggaatcc gtgctaatcc gtaaagacct gcaattagta aactaataac
     4801 tgttaaaaca agataaaaat ggctaagcgt accttgacca ctttgacgat aaagcagaac
     4861 aagtaacaat gggaaaagta gaaagcctgc atagcctagc ccagaaccta tattatggat
     4921 ccaagtcgat agattacaag agctttcatt cgtattgata ctgaataagc cagtaaaaat
     4981 acaatcacct atcccatata aaccaactgt tgcaacaaca agtattgcaa gtgtttttga
     5041 tgttccaaca aataggtgat acaacgcagg caacgaaaga acaaaaaaaa gtccagatac
     5101 aactgaccag accaagaaag cacgtctgac tggactatct acgtcaccaa acacactaat
     5161 tacggtagtc atttgattca gtttgggata aaaaaggcca aggatatacg gtgtcgataa
     5221 atcactcatc acacctaata ataaaaaata aaaaccatat ttttgcaaaa aaatcatttt
     5281 cttcccctac tttttagtga ttattcgata catccaacaa tcaaattttt tctcatttta
     5341 acacatattt ttattctagt tgtcttttga acaatcaata acataaaggt gagtttcttc
     5401 tacattttaa aaaatcgaat ggcactgaag agttcagttg tcagcatcaa tctcctactc
     5461 aaaaaggcaa aaaaacgtaa gtcagatgtt gttgacatct cacttacgca tccattgatt
     5521 gaaaatttct tagtaaaatc tactagccta aaaatttgaa gacttcttct cgatcataca
     5581 gtttaaatac tttctcgcat aaaaattgag taattagtgc cgcaaaaaca gggatgacta
     5641 accaacaaag aacaacaaga aagccattta gccctgcatc taaagaagcc aacggaccaa
     5701 caagtccgac cacgccgaaa ccagcagaag caggcgtacc tgaaatagaa aatagagcga
     5761 caggaatagc tgtaatcact gcactaacta aacaaggaat aagaatcaca ggttttctaa
     5821 atagattagg catcatcatc ttcatagcgc ctaaagcaat cgcaattgtc acacctgact
     5881 tattaacctt ccaagagttc actactaaaa caaaagtggt tgcagctacg cccatcgcag
     5941 ccgcaccagc agaaacacca tctagttgga ttgccattcc gattgctact gttgaaatag
     6001 gagagataat caaaaaggca aaggaacaac caatcaagat agacattaac agcggttgta
     6061 aggtagtgaa gttattaatc aatgatccaa ttgcagaagt aattgctgtt acaaaaggga
     6121 ataaataaaa accaagtaaa cctgcaccta cacctacaac aatcggtgtt aacacaatcg
     6181 acactgaccc aaaactattt ccaatcagta aaatcagccc gacagcaatc gatgctgtca
     6241 gcatcgtatt aataatatcg cctgtaccag caccaacgta aacacccgtt tcacccaaat
     6301 caggtgtgaa tttgactaca ccagaaccaa caaaagaagc tccggctaca accatcattc
     6361 tcatcggatc aaatccaaat tgtaaggcaa tcaaaccacc aatgatcaaa ggtgttgcta
     6421 gttgaaaaat aactgctaac tgtgcgatcg ttaccgcaaa gggatacgca ctaaaatatt
     6481 ttaaaatggc acctaatact gcattaggaa tcaatccaat aatgattcct gttgctgtac
     6541 cagcgaggac tttattcaga aaaattttcg gtgatatttt ttgtgaagtt tccattcaat
     6601 ctctccttta ttttacgttc aaactatctt cttttgcatg gacaagctca gtcaagaaat
     6661 cacaataagg cgtcgcaaca ccatatttct tcccttttct agaaattgcg ccattgatat
     6721 aatcgatttc tgttaatcga tggttcttaa tcaagtcttg atacattgaa ggataatgta
     6781 acccaattgt ttccgggtca aaacaagatt cacaatgtgc aatgacttct gggacatcta
     6841 gttcaatctt ctctactgct gctactttgg caaactcatt gacaatcgtc gccaccattt
     6901 tatgagcagt cgatgttttt cctagctcag ccatattaac gtctaagatt gtgcatagtc
     6961 cgttcattgt tccgttcaca catgctttgc gataaataga atagtgaata ttgtcagaaa
     7021 aatgagcatt taatcctgat tcagacaatt tgtctgctaa tttttttgca gctgcttctt
     7081 ttccatcacc taaattttgt aattctactg aaccacttcc aaataatttg acctgaccag
     7141 gaccttcaag acctgccgtc cacatggtat ttccaatata gatattttcc attggtacaa
     7201 atttctcaat aatatcttca tgcccgatac catttaatag acataagacc tctgtatctt
     7261 ttttgattaa tgattggata tcctgcagca ttttttccag ctgcatcgct ttggtaaata
     7321 gaataatcag atcaacttga tcttcttttt ctacttcgga ttgaagaacg attggtagtt
     7381 ttgcttctac ctctttacca ttaaaatttg cttgtaatcc atgttctttg atttgttgga
     7441 catgttctgc ccagccatcg attaataata cttcgttccc actttggtga agcatcaacc
     7501 caaagcgact tcccattgcg ccagcgcctg caattgctat tttcattgat tcttcctcct
     7561 ttttctttct attatactca tttgagaatc ttttcacaac ctcgttttgt tattcttctc
     7621 ttatttatca tgtttatatc caccattcaa gttattttat cagcaaatca ataaaaactg
     7681 gaatagaatc ccaatttcat cgtaagagaa actagactag caataagaaa atagaaacat
     7741 tcatagaaaa aatctcactc ctctgattat aaaagagggg gcattaacct cgtgtatact
     7801 aaaaccttcc attcattcga tggatgattt tctttttgtg aaaaaatatt cattgtagta
     7861 cgatgttgcg caacacagac ggatggtatt gcttattatt tattaaaatc gaacaataca
     7921 cttatttaaa aacttctttc ccagattttc ttccattttt acagttataa ctagtattat
     7981 catgaaaaat agccttttat tgtctatggg aataataatt taaaattggt acttttttat
     8041 taattagttc atcgcccaat aatctctcca gtttcttagc atctcctgac tcaaccggaa
     8101 ttatccaagc ccagtctaaa taatttttta aaatctcatc atattttgca aatatttcat
     8161 tgccatattc cgtccttaaa attatatttt cagtgccaga caagtgattt atattgagtt
     8221 gataaaaaag atctccatct aaaaaaatat cattagaaga agatttcccc acagtaatga
     8281 ccattccatc agctctatgt gttacataca aataattgta tgacctcatt tcacctaatg
     8341 aatcaattat ttcttgccct ttagctctaa tatattgatc tctattacct atctcactct
     8401 tatttaacaa aagttttttc atgtcaaatt taatcggctg ttttattttt gtaaattcgc
     8461 ttctactact aatccaatcc gagattaatt tttcaacatg tcccagagta aactctgatt
     8521 tattcgctaa atactttttt tgttgtatta aagacatctt tccccgaagc tcaaaattga
     8581 gcctacatcg attctctatc tcttcaaaaa gcatcttatc ttcatcattc ttatattcat
     8641 cattcataaa accaatccag ctcctcgatt aagataatat tttaatctca tacaaaaact
     8701 agctctcatc aaaacaacca ttgttttgat ttctcatgaa atttctttat acttttctcc
     8761 gattataaat tcctaacccc ttgagaaaat tacgcatata tttatctccg caaggcttaa
     8821 aatttcgttg gccttctttt ctcaaaaatg ccccgatttc gccttttgat ggatagattt
     8881 ctgcctcagc aaaaacgtct aaaatatcat cagtggtcat agacaaggcg atttttaatt
     8941 tttttagcaa cacattgttg atatatttga tttccttgct tttttgcggc gtgtctacta
     9001 tcggcttatc tttttgcgga ccacgcgcca agagaatcag gccatttagg aatgcgtcaa
     9061 aaacttggtt gttgatggtt ttttcataca cattattttc tgcattatct ttgttcttat
     9121 cctgtacttt ggtcagtacc gcctgagctt cttctttagt cagtggtaca ccgcctagct
     9181 caaaagcttt aatcaaatcc gtatctttca aatcaatcgc ataacgcaat cgcaataacc
     9241 tatcattgtt attcatttgt tcctccgaag tatttagtct ttactttctt acgtactttt
     9301 tgtcttgcta agatttttag gatcaatgat aaaaaccgca tcacttatga taaggcgctg
     9361 ggtatcatga gtgaatgata ttgctgaata caaatagtcc gaccacatta agttgatcgg
     9421 acttcataag cattttattg actaatactt ttttgcttaa gaattgattc taacatcttt
     9481 tgctaaagtt aagcatccta tagttcctgc attatctgct aacttaggtg tgacaatgta
     9541 ttcttcgata tttggcgtat cgacatagcc gttaattagt tctaaaaaac gtctttgtac
     9601 tttctttttc agatgttctt gttgcatgac accaccacca aaaattataa tatctggtga
     9661 aagcatcagg gtcgtattac aggcacattg tgcaatataa tccgcctcga tttcccagaa
     9721 cggatcttct atagaaagat cttgtccctt ggttcccgta cgcatctcga tagcgggtcc
     9781 agatgccaac ccttcgagac aatcatgatg aaaaggacaa gtaccagaga aagtatctcc
     9841 tgaatgtctc ttaactaacg tatgccccat ttctggatgg ctaaaccctt caacaaaaat
     9901 cccatcttgt atagctcctc caccaattcc agttccaaca gtgtaataaa caacactgga
     9961 taaacctttt ccatgacctg aaacatactc cccgtaacaa gcagcattca catcggttgt
    10021 ccaggcaatt ggaatttcga aatgcttatt caacgttcca acaaaatcaa aatttttcca
    10081 ggcagttttt ggagtagagg tgatatgtcc ataggtcttt gattcacgat ggatatcaat
    10141 cggtccaaat gaccctactc caatgctttc taattgttct ttatagtgat taaagaaatc
    10201 aattaccaaa ctcattgttt cattcggtgt agttgtagga aaactcactc tttctaaaac
    10261 ctttaaatca tcattgccaa tggcacaaac aaatttagtc ccacctgctt ctattgagcc
    10321 atacatttct tactccaccg tccttttatc taggaattcg ttcttccctg tctaatagtt
    10381 tttcgaaaaa ctcatgtggt tccttttggt accaataggc aacagaagaa atatcgtctt
    10441 gacgctcata aagcttgatg tcatcatttc ctagggtttg gaaagtaaca cgtaaatttt
    10501 ctttgaaact aatggggtcg ggtaaatgcc agcggtacaa cccatgcatc ggtaaagcat
    10561 catcgccaaa accatgaact ggatttggat taggttttcc agcactaaaa taatcccggg
    10621 tgtgatctct tttagattga aaaggatagc ccataaatag cgtattaaaa ttttgagcat
    10681 atgccctttc tccaaagttc tttttatgga aagcccaagc gccgccaaaa taatcttctg
    10741 ctcctgtaga tgttattgta gggaaatctt catctccatc tatataaaac ttaaattctc
    10801 cttcccccca ccagtatctt tccaatgcgc acaaagcaaa gtaggttcct acataatacc
    10861 cagtgccttt gattccatca ataagggtgt aatcttttcc ttttgtcgtg atgggttcac
    10921 ggttccattt agcatgaaaa tacatcgcat catccggtac ttcagtcaat gcataattaa
    10981 atgtataaaa aatgtgctta ataaactcag gatgttcatt cgttaaagta atcttagctt
    11041 tttttcgaaa tggcatctca aaatagcaat tcatccctga tgttggattc actacgattg
    11101 gtaaggaatt cacttcgcat cttgtcccaa acccattaca aaaaaaatct cctaatggac
    11161 tttctacaga aggagtggat tcatcatccc aataaatacg caaaattaaa tcacgtaaga
    11221 cgaaactccc agcgattgtt ttgtctgtca ctgtcatcca aaaatgacgt aaaattccag
    11281 ggccttccat ttctgctaaa gtaattgtct ctccagcatt tatcggaaca gaaggtgacc
    11341 cttttctccc aattcctaga tgactcgctg tcattgccgc tttccctttt tcaccatttt
    11401 tattttctgg tgaaatagtt ctactttcct ctcccttgtg gagccatata ttatttaaac
    11461 aattcatttt attcgtcctc tatcctttct tcactttttt gattattcat atagatgatt
    11521 tgttgttgtg cctccgcaat aattgctcca atatctaaat tatcatttcc taactgcaaa
    11581 gcgattgaca ttgccaaggg aagattcatt cctgcaatta cgtgcactct gtcagaaata
    11641 taggtaaaaa acttttgatt tacacttcct ccagccatgt cagttaaaac aattatttcg
    11701 tctgtagtat ccactaactc tttaatttgt tcatctatat catcaatttc ttcgacatat
    11761 gcacataagc tactaatttg attatgcata cctgttaaaa actctagtgt ctctttaaaa
    11821 ccctcagcta gtattgaatg gctagcaatg atcaatcttc tcataattca aatccttttc
    11881 ctttacgcta atattccaaa tgcagccata accatcgcaa aaacaataac gagtagaatc
    11941 aaaatggtca tactgatttt tttcttactg agtagataat atgctgcagc agtaataatt
    12001 actggaatta atgttggtaa aattttgtcc aacattggtt ggattttcat actaacttca
    12061 ccaaaagtga atttaagtgg cgttgaaaca ctgataactg aagcaattaa tgatccaact
    12121 actgttagcc ccaatactga agcagcttca gttaaataat taatcttttc accgtattca
    12181 gtaacaatct tcattccctg cttataacca aattcaaagc ctctcattcg gaaaaagaag
    12241 attaccatga ttaaaatgaa ccataaaaag attcctacag gattattttc taaagccata
    12301 taagccgcaa ttgatcccat gatggttggg atcatcgtca tcaaaagaga gtccccaata
    12361 ccagcaattg gacccatcgt accaacttta aagtcattaa tcgcatcttt tgcttctaat
    12421 ccttgctttt cctccattgc aacacaagca cctaaaataa tggctgccag ccaaggctgt
    12481 gtattgtaat actgaaagtg attatttaaa gcttcgatgt attcatcatc atctttgtat
    12541 aattttctta aaataggtga caatgcgtaa gtcacagatg caccttgctg tgtttgataa
    12601 ttaaaggtac aaactgacat caaccaacga tagttcgctt tcctaagatc ctttttcgtt
    12661 acttgatatt tttcgctcat tttattcatc ctccccaata aacccaacat cagaagctcc
    12721 cgcagaagca gtgatttgtg catctttttc agctctatga tacacattaa atgctaaagc
    12781 gaatcctatg atcgccgctc caataatagg taccttcaaa aaagcggcta agaaaaatcc
    12841 aacaagtaaa taactaatgt atcgacctgt aggcataaag cgtaacaaca tcgcaatacc
    12901 aacagcaggc aacatttttc cggctaatgt tagcccatcc ataaaccaat taggtaaaac
    12961 atccaaaacg taattaacag cagactgtcc taaagtaacg gcaataaata ctggaattgc
    13021 tgcagataaa ccaaagaaaa ttggccccaa ccacaaaaca ctattcattt tcttaaattc
    13081 tcgtttattc gcatattcct gtgatttgcg aacaacaaaa ccatttaaga ttttgacaat
    13141 tacatctagt tgcaccccta acatacctac aggtagtcct attgctaatc cggcttctac
    13201 tccaagctgg ctaccaaatg ccgttgcaat aattgcgcac gttccatagt ccggtacaga
    13261 tgagccacca aaaccggcaa ctcctaatga catcaattga actgtaccgc caactatcaa
    13321 acctgttttt atatcaccca tcaaaattcc tgtgaataat ccccaaaaaa ctgcgtaatt
    13381 tacaaagatc atcggaatcc cataataatc aacatgttta ataaatgcta atatagtcag
    13441 cataataatt tgaaaaatag taaaatgcat tatccttact cctttatcct aattttgatt
    13501 gaatttcctg tgccttatcc gccggaacaa attgtgtaac aatttctaca ccagaatcca
    13561 acaatttttt atacgtgctg atattttcat ctgatgcata ggctctagaa gatagctgta
    13621 atgcattatc tttaacaaca gtaccgccaa caattagtcg gtcaattcta ataccgttat
    13681 ctactaaatg aaggattgtt tccggctctt tcacgatgac aaatactttt tcctgatcgt
    13741 atttcccctt tttaaaattc gcaatcgctg tttcttcagt gataattgat acagccattc
    13801 cagcaggtct agctaaacgc atacttgatt tttggatgtc gttatttgcg acagaatcat
    13861 cgatcaccat cactctgttt gctttagaca ttggtgccca ttgcgtgaca ataatcccat
    13921 gcaataaacg gttatcaact ctcgacataa caatactcat aaatacttcc tcctttatct
    13981 ttcattttct tacaagcaat taccatgcca aaacaaaaag aacttgataa atcaagctct
    14041 ttttacataa tcataaacgt atttaacctc atgaatcggg atgtcgacac tataataatg
    14101 ttcaacgaca ctgaaagatt gcttaaaatg tgtctgaaat ttttgaaatt cctcactcgt
    14161 ttcattgaaa taaatttcat cttccaaagt attttgtttt actaatcgtt caattaagca
    14221 actaatgtga acatataatc cgaaacacgt attattatca aatgaaattc ctagatcatt
    14281 ttgtaagatg tatatagcat ctgaaacccg ttctaataat ttagtcggat ttaatatggt
    14341 caattgctcc attaaattcg ttagactaaa attcttcaga atattattgt tcaaaacttt
    14401 cagttcatta tcattaaagt gtctattcaa ttgattggag aatttactta tattcattcc
    14461 taaaatcata tcttcaattg aaaagaattc tacatcttct agatgtggat ttagtgttcc
    14521 aacaataaaa agaatattgt tctctttacg aagctccgat aaatagtttt cattcactaa
    14581 agagtaatag tcacacgttt ctatgacaag atcagtatcc cttggaaagc tatcttcaag
    14641 tatccttttt aatttaaacg aagttcctaa tccgcttgca caagaacaga caatcatatc
    14701 cttcttaata tttgatgatt cataaatata atccacattt tgattttctt ttacttgttc
    14761 aagaatctgt ttaagcggta aatctaataa tatctgattt gcaacatcaa tagccattct
    14821 tgttgaaaca ttgtttagta gtccgaattt tttttgagcg tctagttcca aatttagata
    14881 aattttctcc agtgaaccca tatcaacaag taaaataatt tcttcatatc cggtacactc
    14941 ttgtaagtat ctattaattt tgtctgcaat ctcactactt gaagtcatta gtggcatatc
    15001 aatggcgtcg tatatatatc tctcaacgat cctattagca gtacttgcta tacaggttgc
    15061 cgttagatct ccatgagcca aaatcagtgc taaacgtttt tgctgaaatt ctttactaca
    15121 cattaactta aataaaataa ccaaaacatt tttttgaatt tttgttaaat aaatatcccc
    15181 tatttgaata aaacgattga ctaattgaac ttctcgacta caccaactat taatatcatt
    15241 gatgaaggaa tataatttat cgtccgatgt taattggatt tgataaatcc aagaattcaa
    15301 aaagatagac gttatttgaa gataataata gtcaaattct atttgaaaat ctctctctat
    15361 ttccttaaca tactgtctaa atcgaatctc ttgataggtg ggatatgtta aagctgaatt
    15421 attttggata tcaacattct tttgtctaaa atatctatca tatttctcaa aaagctgctg
    15481 cttatcagtg tctttcttcc acgtttgata aagatactct ataagttcgt tgatattaaa
    15541 aacttcgtct actttttcca aatcatccat ttgaatatat tttaattcat tctgtctttt
    15601 cataaccttc atgccgttga aattcaccca ttgtggtaaa tgatacatct gaatataaag
    15661 ttcttccaaa tcatctaaga aataaaaggc attggcacag gtcaattgaa tgatattttt
    15721 caaattgcca acattttcac taaagcgata gtttaataaa aaatctaata attgttgaga
    15781 tatcagtatt ttctttgcta gtctttcttc ttctgattta aaaaatatct tgattaactg
    15841 ttttctttca taaagagaac gactgtctaa tgttggaagt gttagcttta caggtattct
    15901 tctcaacaaa gttgataata gtttatcctc aggtggttct gatgttgcaa atataatcca
    15961 gcaacttgat gtgtaccagt tttcattatc acccatccga tgaaatttcc tcgtatccat
    16021 aaactgaaaa agtttctcct gacattcatt gctcaatgca tgaatttcat ctaaaaataa
    16081 gactccatta ttagctaatt ctgctaaacc ctttcttcca tcttcggctc ctgtatatgc
    16141 accttttaca tgcccaaaaa aattggacaa gaaaagttct ggattattgg catattcact
    16201 acaattaact gaaatgaaag atgccttctc ttctatataa ttattggcca acaaatcctt
    16261 atacacataa tttgctaaat agctttttcc agttccagtc tcgccatgaa gtaaaatggg
    16321 taagccattt ggcggatagt agattgctgc tttaatttga gcaattacat ttcgcagact
    16381 tccttgactt ccgataaccc tttctattcc aaccctaaaa gagctatcat accatttttc
    16441 cgcttcctct gaagagctaa aaatttgttg atttggaact ttctccggta aagaaaagaa
    16501 gattactgga cgagtattga tttttccaaa ttctccatct tttacgaatt cattcaaata
    16561 ttgagagaca atattacgac taacatttaa tttttcagat attttctgtg ctgtattgtt
    16621 cccccattta tttttaatct cagagttctc tatgacataa ttgacaatgc gttcttttaa
    16681 ttcttccatc acagcacctc ctcgacacta ttcgaatttt gccgacagta tttttccata
    16741 aatatgactt gctaaaaagg ctttgtttag atcattcaat tggaacgttg cttgttttgc
    16801 ttgatccgct aagacgttga aaagatactc tggcgaaatc gcatacccca aaatatcaac
    16861 gattgttgca atcagatctt cttttgattc ttcatctgaa agtaattcac gatacaacat
    16921 cgtttgtttg cttactgtgt catattcctc taaggattca tctgctaaaa gaaccacttg
    16981 ttccaacaaa cggtcggata aatatttata gaaaatcagc cccaataaat aatttttata
    17041 ttctgacgca tccattttac tacggagatt atctgccgca ctaaataact tctgatttaa
    17101 ttctgcaccc aaaaagatac actcctatct cttatttaaa gttgctatat accctcttat
    17161 tttagcatac tcagttttag aaaaagcaaa aaaataaaga aacttattct tgataacaag
    17221 ctacgtgacg tatatcccta acacaaaaat agccatatcc tttggtttgg atacggctat
    17281 ttgatattac tcattcaatt atcaaatcac agtaggtgtg aatctcatcg ggatcggcaa
    17341 tatctacttc tgattaggat caaaggtgat tttctcaata aaggtgtgat acaaagcttt
    17401 aacttgcttc ttctcactct ggataagaaa gcaatccaaa ctagtcaata tctgttggac
    17461 atctttgact tgaatcggtt ccccttgttg ttgaaagatc cctaagattt cattctctcg
    17521 aatttgtaac tgtcttcgtt tttcagtcaa ttcatttaaa cgatctttta acatcggaaa
    17581 caattcggga ctatcttcca aagcagcttc ctgtttttgc tctaatagtt agattttgcg
    17641 taccattttc ttcttttatt ttccttcgat tgctgatttt aatatagtct aaaggaaatt
    17701 tatatttatt gtattcttta taacttcaag agaacttcgt ttgagtatta ctgtattttt
    17761 ttaattccat aagaaaatta gttattacct gccacctaat cagcaaatct gaacctgagt
    17821 caatataatt aatattattt tccatctcca acattgtaag tactgttggt gttaatatag
    17881 ggactttgta caattgtatt tttttgattt tatcatctgc acaaaggaga ttttttaaat
    17941 ctaatccttc ttttatttca aaagttgaat tttttaaatc tataagagta aaaccaattc
    18001 cttcatactg aatttctgaa aatcgaattt ttctcccagt tgatagatca aggggcagaa
    18061 aaccttttat tacattgtga gtgtcaccat catttacaat gtcattagga attttgattt
    18121 tagaattctg tggagcacct attggaagat aataattcat tataattttg tagtcttcat
    18181 gaggaaataa agtactttta ggattgctaa gatattctcg taccgaatct aagaatggct
    18241 tatccattga atcttcatcc ccatttaaac taaaaaacat tcctacaatt gcttttgcaa
    18301 ctctaagtga attcattttc tttattgaat gtgttaaatc agacttacca ttctttaaac
    18361 ttgtattgtt ataaaggaaa tttttattgt cttctgcaaa tctaatatat tcctcaacat
    18421 actcactacc aaaaaaacta ttacatttac tacataacg
//
LOCUS       DACCXO010000047        18377 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.048, whole genome shotgun sequence.
ACCESSION   DACCXO010000047 DACCXO010000000
VERSION     DACCXO010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.048"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(103..1611)
                     /locus_tag="IVY70_001522"
     CDS             complement(103..1611)
                     /locus_tag="IVY70_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP7958657.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            1770..1961
                     /locus_tag="IVY70_001523"
     CDS             1770..1961
                     /locus_tag="IVY70_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958658.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2164..3081
                     /locus_tag="IVY70_001524"
     CDS             2164..3081
                     /locus_tag="IVY70_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP7958659.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            3074..4147
                     /locus_tag="IVY70_001525"
     CDS             3074..4147
                     /locus_tag="IVY70_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958660.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            4295..4813
                     /locus_tag="IVY70_001526"
     CDS             4295..4813
                     /locus_tag="IVY70_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958661.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            4794..5186
                     /locus_tag="IVY70_001527"
     CDS             4794..5186
                     /locus_tag="IVY70_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP7958662.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            5262..6197
                     /locus_tag="IVY70_001528"
     CDS             5262..6197
                     /locus_tag="IVY70_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP7958663.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            6330..7109
                     /locus_tag="IVY70_001529"
     CDS             6330..7109
                     /locus_tag="IVY70_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP7958664.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            7102..7419
                     /locus_tag="IVY70_001530"
     CDS             7102..7419
                     /locus_tag="IVY70_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP7958665.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            7663..9273
                     /locus_tag="IVY70_001531"
     CDS             7663..9273
                     /locus_tag="IVY70_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7958666.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            9430..10278
                     /locus_tag="IVY70_001532"
     CDS             9430..10278
                     /locus_tag="IVY70_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7958667.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            10509..10988
                     /locus_tag="IVY70_001533"
     CDS             10509..10988
                     /locus_tag="IVY70_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958668.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            11021..13249
                     /locus_tag="IVY70_001534"
     CDS             11021..13249
                     /locus_tag="IVY70_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP7958669.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            13378..15021
                     /locus_tag="IVY70_001535"
     CDS             13378..15021
                     /locus_tag="IVY70_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP7958670.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(15094..15639)
                     /locus_tag="IVY70_001536"
     CDS             complement(15094..15639)
                     /locus_tag="IVY70_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP7958671.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(15662..16147)
                     /locus_tag="IVY70_001537"
     CDS             complement(15662..16147)
                     /locus_tag="IVY70_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7958672.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(16137..16949)
                     /locus_tag="IVY70_001538"
     CDS             complement(16137..16949)
                     /locus_tag="IVY70_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP7958673.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(17088..18200)
                     /locus_tag="IVY70_001539"
                     /pseudo
     CDS             complement(17088..18200)
                     /locus_tag="IVY70_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
     gene            complement(18221..>18377)
                     /locus_tag="IVY70_001540"
     CDS             complement(18221..>18377)
                     /locus_tag="IVY70_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP7958674.1"
                     /translation="SSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGT
                     LRYSLGY"
BASE COUNT         5978 a         3343 c         3666 g         5390 t
ORIGIN      
        1 taaaaggttt aaaaagtgta acgtcttagt aaagtctata aaaaaacgaa actatcgatt
       61 aagctaatag tttcgttttc aacatttata agtaaacttt ttctattggt aatatagatt
      121 ttcggaaggg tagacaggtg cgcaggtaat atcgattcct agttttttca atgtttgttc
      181 atcattttta ttgaccatca cagtagagtg ggcttgtgct ccctcaagtt cagctagttt
      241 atcataagca acttgagcga ttggattcgt tactgcactg attgataaag cgatcaagac
      301 ttcattgatg cttagtgcat ccaaagagga atgaagtgtc tttgatttca atccttgtat
      361 tgtttcaaga actaatggtg acaataaagg aatgtcatct gcgatatggg ctaaatgttt
      421 tactgcattt aagataactg ccgctgacgc atccatcaat gaacttgtac gcccggtaat
      481 gatgacttca tctggtaatt cgattgcaat cacagcagaa ggatcttttg taggactttg
      541 cgcttgaatc ttatctgcat agtcatgggc agggactacc acgtttcggt cttcaggacg
      601 taaaccgact tcttccataa tcagtttgat tcgattaact gcttcttcgt cgatcaatcc
      661 ttttttgaag tcgcattctg tttggaaata acgacggatg atttcttgtt tagaggcttc
      721 ttggacaacg gtatcatcca cgatcccaaa tcctacacga ttcacaccca tgtcagtagg
      781 agattgataa acagattctt taccagtaat tttttcaata atacgtttga ttacaggaaa
      841 tgtctcaaca tcccgattat agttcacagc tactttattg taggcatcaa agtggaagga
      901 atcgatcata ttgacatctt ttagatcgac tgtggctgct tcataagcaa tgttcagcgg
      961 atgtttcaac ggaacattcc atactgggaa agtttcgaac ttggagtaac cagctgcatg
     1021 tcctttttgg ctttcatgat acagttggtt caaggaagta gcgagctttc cacttcctgg
     1081 gccaggagct gtcactacaa cgattggttt agttgtttca atatactcat tttttccaaa
     1141 accatcttct gagacgattt tttctacgtt gactggatag tcttcgattt ctgagtgttt
     1201 atagaccttg atgtttcgtc tttctagttt attgataaag actttcgtgg caggctgtcc
     1261 actatagcga gtgataacga cactgttgat taataagcca taaccacgta attcatcgat
     1321 taaccgcaag atatccatat cataagtaat cccataatca ccacggatct tattacgttc
     1381 aatatctcca gcatagacgc aaatcagtat ttcagcttga tctttcaatt tttgcagcaa
     1441 tttgattttc gcatcttctt caaatcctgg aagtacacgt ttcgcatgct tgtcgtcaac
     1501 tagcttccca ccaaattcta aatataattt gtcatagtgg ttcactcgtt ccaaaatata
     1561 cgctgactgc tcttcaatat atttttctga atcaaaacca atttttttca ctaggttccc
     1621 tccttaagta ctaaagcatt ttttcatttt tttgaggatt cgtcaactgg aagagcgaaa
     1681 ttcctcaaaa aaacaaagaa aatggtttaa accaacttgt ctagatagtt attcattggt
     1741 atgatagttt ttgataagga aggtgctaca tgaaaaaaag acagaaaaag aaaaacgcat
     1801 ataaacacta tatccgttcg atttttaccg gttatgaaag aatgcttgaa gatcccgaat
     1861 tggaacaatt gacgtttact tatctaaacg aagaaacgca gctaactcgt gatgagcatc
     1921 agcgcattca tttcacaaca agagatctgc ctagtaaata aaaaataaga taaaggtcat
     1981 cggattcgat tctgtcctat cagatggaaa gactagttga tcgaaaaaaa ggctgtacca
     2041 aattttggtg cagcctgttt tatggagcat tgcccgctct tttttataac aattttttac
     2101 agaaaatggt actattgaag tattaataag tgaaaaacag atgagtaaga ggaggcaagt
     2161 agtatgaaag agccagatac aaatgcaaat tatgagccag acgaactaga ggagacgaat
     2221 gcggatcagg atacaggaca agaatcgtct tctttttacg atatcaaaga aaaagcaagc
     2281 atgaatcttt cccaattatt agaagactta caggcttttg aaaaagctgt ccgcgaagaa
     2341 gatatccctg agatctaccg tttatacaat ggccgattga atgaagaatt aaaaagaagc
     2401 tcaaatgaaa atcacgagat cgatcaattg ctgatgcgta agatccatga tcgtttttta
     2461 caggaatttc cttttatgga acaagtagaa aacatctcgc caacgatgag ctattacaaa
     2521 atcggcactt attaccatga tcggcccacg gtcggtattg atgcaagtct gccagaaatt
     2581 tttgttttgc ctaagatcga tgaagagtgg gagaaatatt cccaaccgga gtcaacggac
     2641 tatgacaaaa aaatcaatga gctagacgca aaggtgatca ctgctcagac agaaattgaa
     2701 cgtttagatg aacaaataaa agaaatcaat cgacaaatga ctgatttgaa cgacaataaa
     2761 ggattcttga atcgtaaaaa aatcgaggaa gaaattcaag aattagagaa gaagaagcaa
     2821 gtactatcta atgaaaaatt aggctggctt ccttatattg agacgcctga aacaatccag
     2881 cagcaaaaag aagcattgaa gcaagaagca agagcagacc agcttcgtgc agccatcgta
     2941 gaaaaagaac aaaggcaaat cagacgctat ttcggtagca aagagggatt cggtcaagca
     3001 atccatgaat tcttgatgaa ttatcttggc aatgagaatc ctaataatca atctaatgaa
     3061 ggaggttccg agtatgaatg aacacgatga agcagaatgg acaaaaaatg aagaacaaac
     3121 agaagatcat gaagaaacga acacttcatt ggaagaacaa acaagtaatc ctctggatga
     3181 cacagaagaa atccctatgc tagaggaaac aaaaatacac gaaaaagaaa aagtaactcc
     3241 gacgaatacg aatactgcag aaaacaccca tgcttacgaa ataactgaaa atcccgggta
     3301 tttagaagga aaagagcaag atcatcaaga tatcgtcagt ttgattgaaa cagcggaagc
     3361 agaattagcg gatttacgta tgagaaaaga tttgatcgaa tcagattttc cacaattatt
     3421 agaatcatat gatcgtttta tattgaatga agaaccaatc tccacgatcg gaaagacaag
     3481 cctgatcgaa tacttcacgt atgaattact gaaaccgttg aatgatattg gtctggaacg
     3541 gtcaaattca gcttatgaca catggaaaac aagacatttt tcgatcaatt atacgttgaa
     3601 tggacaaaac gaattagtct tttcctttgt attaccgacc atcgatcaac gataccaagt
     3661 ggaatcgatg agtctcctaa acgtatcacc agagacaatg gaaatcaatg tccaagatga
     3721 ccgggtgctt tctttgatcc gacactggtc agtggatcga gtattctctg cagggcagat
     3781 cacgatattc aatcacaaat tgaatcaatt attagcccat gcaagaacac ttggttttac
     3841 cgttaatcag acattactag acaatacgaa gccacttcgt ttgaatcttc agagcgaatt
     3901 tgaattgacg aatgaagtat tggatgatat ttttatcgtg gcaatgaaaa atccatccta
     3961 cgatatggaa aagatcaacg aaactactta tcaagtacga ttagataaag gacagtcttt
     4021 aacgataaca acaaacgaaa aagatcaaac agtcttgtcg atctcatctg gagactaccc
     4081 acgatctgtc attgatttct ttatcaatta cgaattcttg gtcccattaa tggtgagaaa
     4141 gtcctgattg gataagttgg attaccagat gtcattcaga taattctgtt ccaaaggact
     4201 tgcggcagtt atccagaata attgtcacaa gtcttttctg tatgtactaa atggacgtga
     4261 ataacttgct gatgaaggag ggatttcatt aggaatgtcc acaaataaaa tagcacagct
     4321 tagcttattg tctgctgctt gtgtagcagg taggatcgct tttcaattta ttccgaattt
     4381 ccagccaatg actgctattt ttttatttat agtactttac ttaagcttaa aagatgcgtt
     4441 aattgtcatg agtctttcta ttgcgatcag cagtttttat tttggtgttg gaccatgggt
     4501 catcggccaa tggatcagct atactgttgt tctgagtttg ttttcaatcg tctgcttgag
     4561 aaaaacagtg caaaggaatc tatggttaaa gggagtctgt ttttttctgg cgggcatggt
     4621 ttacggaata ggtatgaccg tttttgatac gctgttatat cgtttgcctc aaccttggat
     4681 ctattacctt caaggggtgt cgtttgattt gatgcacagt ataggcaatg ttgtgttctt
     4741 tcttgttttt ctgccagtcg tcaaacgttt ttacaatcat tcaggaggaa aaaatgaaaa
     4801 aaataattta tagtttgctt gcaatgagtg tcagtatact cattttgaca agttgcagta
     4861 caacctatac acaatcggct tccaaccaaa aggaggaagt aaaaacatcg caaaaagcaa
     4921 caatcacgtt acaagaaaat ggtcaaaact tttcggaaaa agaagtgttc ttcaaaaaag
     4981 gcgatgattt actgagtatt ttgaaacgaa attttgaagt caaagaagat aacggcttca
     5041 tcacttcgct tgaagggcac tcacaggatg agaaaaatca actctattgg atgtttactg
     5101 tggacgggaa aagtgctact actggtgcca aagagatcaa attgaatgat ggacaatcgg
     5161 tcattttcaa tttgtctaaa ctataagttt cataaaaaac tgttaaaggg cgaatattgc
     5221 cataattcag tgagttcagt tgatgaaaga aggaaaataa catggaaaat tttaaaaaaa
     5281 gcggaatgtt ttccgtaatt gctgctctag gggctaatat tctagttgct atcagcaaat
     5341 ttgtcggcta tgcaatcagt ggcagtgccg ccatgctaaa cgaaagcatc cacagtatcg
     5401 ttgattgcag caatcagatt ttgctgttat tcggtgataa acaagccaat aaaggacaga
     5461 gcgagttgca tcaatttggt gaaggacgag caaaatactt tttcagtaca atcgtcgcaa
     5521 tgatgctttt cttcggcggt ggggcacttg gggtgatgga agcttttgaa aagttgacgc
     5581 atccatccca tgaagtgggt aatccgatca ttgttatagc aatactgtta tttggtttgg
     5641 taatcgaagg aagttcgtta cgcgtggcaa tgaaagaaat caaagaattg aatactgaaa
     5701 aactttccac gatgcgcttt ttgcgcgaaa gtcgacacag tgaaatactg attattttta
     5761 cggaagactt gtgtgctgta atcggtttgg tcttggcttt agcaggaaca atattgactt
     5821 atgcaaccgg gattgctgca tttgatgcga tcagcggatt gctgatcggt ttcttgctga
     5881 tgggagccgc cattttccta gcaaaagaat tttatagtct gatcatcggt gaaagcgtga
     5941 cagcaagcga tttatcaaaa atcaagttag cttttgaacg tcctgagatc agtcggctga
     6001 tcgatataaa aacagttcat ttaagtccaa cggagatttt agttgcagca aaagcagata
     6061 tcgttggtaa caaagagaat gaagcttact cggtcatcaa tgatattgaa aaaaatatgc
     6121 gtcagtctct tcctgacaag aagatgtata tatacattga aacagataaa tatgacccaa
     6181 attattctcg taaataaaac gtttattcat gaattgaagc aaaaatgaga aaaatataat
     6241 cttgttttta aataaaacgg ctacaattta gcgaatttat gttacaatga gtccgctgtt
     6301 tatttgttgt aattaaagga gatattttaa tgaacaaaaa gaaaagtgcg ccatggcttc
     6361 aagctttgca tgcagtgatg ccactttgta taagctatat accagtagga ttagcttgcg
     6421 gagtattgtt gcaaaaagtt ggatttgatc ctctcttatc aggactgtta tcgattctgg
     6481 tcttttccgg tggcgcgcag tttttagttg catctatgct tacgacgcaa gcatcatttg
     6541 caacgacatt attgatggtt ttctttttag aattacgcta tacattatta ggagcaagtc
     6601 ttgccggttt tatgaaaaaa gaaaagcgga cgtttttggc tgtcttttct caatcactga
     6661 atgatgaaaa ctatgcggtg aactacttaa agttttcaac tgacccttcc tggaataaaa
     6721 gaaaggcttt atatgtcaat tggttctcaa tgactgcatg ggccggtagc aatatgatcg
     6781 gtaatttttt cggctcggtg attcgagtag atgctgacct tgtccatttt gctttgacgg
     6841 ccatgttcat tttcatgttt atcatgcaga tgaaaagcgc actactgatc tttacaggtt
     6901 tattctcagg cgtattaggg gtcgtattca tggtattatt ccaaaatact ttcggattga
     6961 ttgcagcaac agtcattgct tcatttgccg gatttatgct ggagaaggca ttcaaaaaag
     7021 aaaaagctaa gaaactaaga gaccgcggta aagatgtcaa tgagccgctc ttcaacttcc
     7081 cggaacagga ggccaatcac catgactaaa ctatatttgc tacttttagt tgcttgtctg
     7141 ttcatcgtgg catatatccc gcgcttgttc ccgatgcttt attttactca tcgaaaagtg
     7201 ccatcctggt tcagtgactg gatgaaatat gtaccggtgg cattatttgc ggcgttagct
     7261 tttaaagatg ttttcattac tcatgaacat ctggatattg cttggaatat caaaatagct
     7321 gcaatgattc tagtagcagg cgtggcttac aaaacacgtt cgatggcgtt gtcagttttg
     7381 acaggacttg cgtctgtctt tttgctgtct atgctttaaa ctgttttatt ttattaaaaa
     7441 cagagattgc gacataagtt gttcaaactg atctatccga actgcatttg caacagtcaa
     7501 ttctggatgc aagtttattc gtagggataa aaaagattag tgttggctta tgatgcaagc
     7561 tcttttttat tttaggaatt agagagggat ttgtccttct atgatttttt gctataatag
     7621 ggaagatgtt tattttatga aagaatagtt gagggaattt aaatgaataa aaaattgatg
     7681 caaggaacat tttggctaac ctttgctaat cttctatgta aagttttagg ggtagtctat
     7741 ttgattccct ggctaagtat gatggggaac aatcaagatg gtatgcttgc tacgacattg
     7801 tataatgttg gctatcttcc ttatggattg ttcctgatgc ttggtacagt cggatttcca
     7861 aatgcgattg caaaaaaagt agctgtagcc acaaaagaag gagataacgc tgcttgtcgg
     7921 ctgattttta gaagtacgat caatatcatg tttgtcatcg gtatcgcctc tgctgcactg
     7981 atgtatttat ttgctccttt actagccgga atcagtccga ttgctaacgt taataatggg
     8041 atactagcta tcagaagttt atgcccatct ttgattgcta tcccaattct tagtgcaatg
     8101 agaggatatt tccaaggaaa aaatgatctg cggccttatg gaacttcttt gattatcgaa
     8161 caggctgtgc gagtgatcgt tatcttggct ggtacttatt acttgcgcgt attgactgac
     8221 ggaaccatat tagaagctgt attgatcagt acagtcgctt ctttcttcgg tgggttagct
     8281 gcgattatcc atatgtatat cgtcggacgc agaaaagact tcttcgaact taaagatttt
     8341 ttgatttcca gccgttattt cgaaagagaa aaccgatctg cctctgtctc tatcattcgg
     8401 gaaacactac cgtttatttt tgtcggctca gtcattacta tcgttcaaat gatcgaccaa
     8461 gtaacgatga aaccactgct tcactttttc cgtccagaga tcgcagatca gcaactggaa
     8521 ttcttattca gccgtgcttc tgtcaatcca aataaattga cattgatcct gatctccatg
     8581 gtaggaacag tagctataag cagcttgcca atattaagca cgatgaagaa aaaagatcgg
     8641 atacaaatcg aaaaaacagt aggagatagt ttttcgattg cgttattgat tcttcttcct
     8701 tcattgactg gaatgtcttt attagctgga ccactataca cattgttttt tggctatgat
     8761 cctgaaagtg tcggatattt ccaaatggct ttgctagctt ctttgttttt ctctttattc
     8821 acgatcctat ctacgatgct tcaatctttg aatcaccatc gttttgcgat ccgattaact
     8881 gtagaagcaa ttatcctgaa agtcatcttc caagtaatcg gattaggatt agttggcgga
     8941 tacgggatga gcttatcaaa tggcttagca ttcggtattg tctttgttcg cggctatcac
     9001 tttatgtgca aagaataccg aatagcacct ttagcaaaga tcagcaattt tttcttaaaa
     9061 actttccgct ccaccctgat tatgcttgca gtatgttttg ccgttttctt tttgctgaat
     9121 cagcgattgt caatggaatc aaaaagctat gcaatgattt attgtgtggc tgtaggaagt
     9181 attggcggct tagtattttt attttctcaa ttcggaaaaa atgggtttcg aatgttgaaa
     9241 aatgttaaac atcatagcag aagcaaagaa taggttaact aatcgttttt tgctgtcact
     9301 tgaaaaacgg caactcgtat gattacgctt gtcgtttttc tattttccaa ttttagtttc
     9361 atatctaatc ttacttatga tatagttata aggtctggaa aaattctttg aaacgaggaa
     9421 attaaacaca tgaaacttgc agtagttaca gatagttcgg catatctgcc ggaacgtatc
     9481 aagaaccatc ccgacttatt cgttattcca atccctttga ttatggatgg gaaaatttat
     9541 aacgaaggaa tcgatatcga agcggatgaa tattatggac ttttaaaaaa cagtaaagat
     9601 tttcctacta cttcacaacc tgtcgtaggg gaagttttgg gtttatatga ggaattgaaa
     9661 gagaaagggt atgacacgat tttaagtatc catctttctt cagggatttc tggctttgtc
     9721 aacacattat ttgcgatgaa agatgacatc agcggagtaa cggtcattcc ttatgactct
     9781 aagatcacta gtatgccgat gggacacatg gtagaagctg ccttagactt aaatgatgaa
     9841 ggtcagtccc ttgatgaaat acttgcacat atcgatcgaa tccgagataa tacctacgct
     9901 tatctgattg tagatgattt gaacaattta gtacgaggag gacgtttgac aaatggtgca
     9961 gcgctgattg gcagcttatt aaaaatcaaa ccgatcctga cattttctga agggaaaatt
    10021 gttttattcg aaaaaatccg ttcaagtaaa aaagcatttg ctcgagctga agaaattatt
    10081 ggtagaagaa ataacgaaat acaaccgcca gtcaaacttt acgtgataca tgcaaataat
    10141 ttagaagtag cgattgcgga gaaagagaaa ttgcagaaaa aatatccaaa tgcagaaatc
    10201 gaaattggtc atttcggacc agtcattggt acacatttag gtgaaaaagc catcggcatt
    10261 gccatttcag cgcaataatt ccttttattc aaaaaacata ccaaaaatat tccaacatca
    10321 tcttggactt tctacatgtt actaatgagc gtgtaaaaga acgagatggt gtttttattt
    10381 ctatcaatga agctcttttt ctttgcaatc ctcacttcaa tcctctataa tgtcgcatat
    10441 agtttagtaa atgagtaaac tactaattta cttatttact aaaaataaaa ggatgaggag
    10501 ttaaaataat ggaaatacca acaaatttaa aacataaacc agtaatcgtt gtagaagact
    10561 atgataaagt tgatggaaga aatgcattaa atacggatgc aaaagggctt tcactgggcc
    10621 ttgctcagtg gaatgatcgt ggaaaagtcg atatctcagg caaggtatgg cgacatactg
    10681 gagaaaaatg gtcacgacag tcggaagaat taccaatcac acgtatttta gatttagcga
    10741 tcttagctgc acaaggaagt ttatacttcc aagatgctta tcgttatgaa aaattttatg
    10801 atccagaaaa tccagtcgta gacatcattg gattacaagg gaaccgaatg acggtagaag
    10861 tagataccaa aaatccaaag atcgatgaag acatcatttt gtattacgat acactacaaa
    10921 aagacggaga actgattggg gaacgattcc gtattttaaa acgtctttta gatgagctag
    10981 gatattaata gacagagatg atcaaaaggg gaagagaagc atggagaatt tctcactaac
    11041 agaaaaccga accttttcac ggctagaaaa gcaaatgatc tggaagaaac cgaaaagcca
    11101 tcggacaagt gcagaagaat ggcggatcgc ctccgaaatc aaagggaatt ggcaagatcc
    11161 tgaaatgaaa atcagtaacg tacttttgga aggagatgcc gggtcgggga agacacaatt
    11221 agccaaagca ctatctgctg atctgcaact tccctataca aaagtgacct gttttgcaga
    11281 tatggacaaa tcagatgttt tcggagcctt attacctgta gtagattcta ataaggaaca
    11341 agaccaagaa ttactagaag ctatttacca aacagataca cttgaagctg tactgacact
    11401 gatccagaac cattacggca ttgatccttt ttctgctaaa gaaaaattag cagagctagt
    11461 tcaaaggatc gaagctgatt atccagatgc aatccattac agctattacc cttctgaaat
    11521 cgttcgagcg attgaaaaag gatatttatt agagattcaa gagccgactg tcatccgaga
    11581 tgcctctgtt cttgtggcac ttaactcagc gctcgaacca aatgggatgt tgaatttacc
    11641 gacaggaatc attcgtcgcc atccagattg cgtcattgtg atcactacaa atcgaaatta
    11701 ccaaggaaat cgcccattaa atgaatcttt aagagatcgg atgcaacatg cggaaaaaat
    11761 ggatcttcct gccttagaag tcatggcaga acgtgcgatg gcgaaaacta tgatccaaaa
    11821 acaagaactt ctcttaaaga tggcagagat tatccgtttg ttggatgaaa ctgcaaaagc
    11881 taacgccatc aaaggtgtag caggaatgcg ttcttatttt tattgggcaa atacctataa
    11941 gcaaggacaa gatttactcc agtcgattta tccaaaagtt ttgtataaat tatccactga
    12001 ttcagatgaa ttggcaattc ttgaacaagc tttgtctgac agtggattgc tactggaact
    12061 tcaggacata ttgagaaaag aaaaatgggg tcatcctcaa tctgagcata caaaaggaag
    12121 aaccattagc gccgaagaag caaatgaacg aaacatcact gaaaatgctg aagaaattct
    12181 gcgcttagat gaaaagaaat cgttgtctgc tgaagaacca aatgagggag aagagaaaca
    12241 agaagaaaca atagaaaaaa acgagattcc tcctcaaatg caaaaagaac gcagtagcga
    12301 tatcgaagga aaaaatcaaa aagaacaaca aacaggagat gattctcaag aaatgtctgc
    12361 ttctgacatg gaagcacatg ataaggaaat caagaagcaa ctgaataaag aagcgcgaca
    12421 aatcatgaag gaaacgattc atgaaaaaga aggtctcatc gttcaccgac cagctttcca
    12481 aggaaaaacg ccggaagcac gtgagctaca catgcaagta atgccaatag tagaatcact
    12541 tagccggcaa attttagagt tactggataa tgaacaaaca gagacgtacc aaaaaggaaa
    12601 atacgaaggt cagcggttca atgcaagcaa agtagcatat ggtgatttga gaaattttga
    12661 caaaaagaac ccgccacatg agcaaccttc tttagcggta gctgtacgga tcgatgaatc
    12721 tgggtcaatg atacgggatg atcggatcga agcggcaaaa aaagcagcga ttgccattgc
    12781 cgaattttct aagaaagtcg atatcccatt gttgatttac ggagatacag cagaccgttc
    12841 gtcaagagaa aagacttctt tgttttctta taaagaattt gaagacggat ttcaatggtt
    12901 aaatgaaaaa ttagtcacta tgaaaccaag gcaaaacaat cgtgatggtg ctgcacttcg
    12961 tttagcagca gaaaaattaa acagacaatc cgcaacaacc aaactgttgc ttaatattag
    13021 tgacggtcag ccaaaagcgt tgccggatta tacaggcaaa aaagcaaaag aagatatcca
    13081 gtctgttttg aaagaatatg aacgtcaagg aatcctattt gtctccgcag ctattggcca
    13141 agacaaagaa gaaatcaagt cgatctacgg tgaaagtcgt tttgtagata ttactgactt
    13201 gaatgaattt cccaagcaaa tgattcaact gattgcccgc tatctatgat aaaataaagc
    13261 tcaacgaacg cacttagcag attcgttgag cttttcgttt ctagttaccg gtaacaagga
    13321 tacttctaaa atagaatcgg ttataatcaa gagggaccgt taaaggagtg aaacgatatg
    13381 atacaagaac aaaaatggtg gaaagaagca gtaggctatc aaatctatcc tgctagtttc
    13441 aaagatagca atggcgatgg tcaaggagac atcaatggga tccgtgaaaa acttaattat
    13501 ttgaaagatc ttggtatcgg ctttatctgg atcaatccga tctatcaatc tccatttgtc
    13561 gataatggtt atgacgtaag tgactatcaa gatatagaag agagattcgg aacgatggaa
    13621 gattttgatt tattgctaaa agaagcacat gaattaggga tcaaagtgat tatggatctt
    13681 gtcatcaacc atagttcgga tcagcatcaa tggtttgaag aatcgaaaaa atctaaagaa
    13741 agcccgtatc gcgattatta catttgggtc gacggggtag atggcaaaga accgaataat
    13801 tggacatcga ttttcggcgg ttctgcttgg gagtattctc atgaaactgg acagtattat
    13861 ctgcatgttt ttgcaaaaga acaaccagac ttaaattggg aaagcgagaa attgaaagaa
    13921 gaactattca atatgatccg atggtggttg gataaaggga tagatggatt tcgtttagat
    13981 gcgatttctc atgtaaaaaa agatgaatat tcagtaaaag ccacagagaa ccctttctca
    14041 ccctttcaaa atgtctcagg aatcgaagaa catttgaccg atttgaaaca tgtttttgaa
    14101 gagtacgata tcatgactgt cggagaagca agtggtgtca ctgcagaaga aggaccacaa
    14161 tgggtaggga aagacggtta tttcgatatg atattcgaat ttgatcatat ccatatctgg
    14221 cagcaagaaa aagaagggca attagatgtc cttaaattga aacttgcatt aagcgcttgg
    14281 caaacttcat tggatggtat cggatggaac gctttatata tggaaaacca tgatgttcct
    14341 cgtgccgttt cagtgtttgg agatactcgt cccgattttt gggctatgtc tgcaaaagca
    14401 atcgctatga tgtatttctt tttgcaaggt acgccattta tttatcaagg acaggaaatc
    14461 ggaatgacga atatgccctt tgaatcgatc gatcaagttg atgcagtaga ttcgaaaaga
    14521 ctttataaaa ggctgcttgc ggaaggtaag acaagagaag aagcattgga tatcattcgt
    14581 gaaacaacga gagacaacag caggacaccc atgcaatgga cttcggaaca atatgctggt
    14641 ttttctacac atgaaccttg gctgatcact aatccaaata ccaaaacgat caatgtagaa
    14701 caacaagaat atgaaccaga gtcggtcctg caattctata agaacatgat ccgcattaga
    14761 caaaccaata aaggtctgat atacggttct tataaagaat atcttcatga acatccacaa
    14821 ctttatgtct atgaaagata tttagaagat gaacaatatc ttattatggt aaacttaaca
    14881 gaatcacttg ctgactatga acttccaaaa gaagcagatc agtcctggac attactcttg
    14941 tcaaattcct ctagcggaga atttgaagct aaaggcatac tagcacctta tgaagctcgg
    15001 ttatataaaa caaacaagta aaacttcctc gttaaagttc attagttgga ctttgcgagg
    15061 aagtttttca gattgctctg tcaattactc atttcaataa ttcgctctag gaccaccgat
    15121 atatttagga cgagagcgaa gggctgtcac gtgagcttgt ccaagggttt tcaaacgagg
    15181 tctgacaggt acttggacat gtttgatatg cataccgatt gatgtgtcac cgatatcgat
    15241 accggcttgt ccaactacgt gttccacttc gacaggatca ttgaactggt caaaagcagc
    15301 aacagaacaa gcacctccag catgtagtgc aggaacaaca gagacaattt cgaaattttt
    15361 tgcttctgcc actgttcgtt ctactactaa tgctcggttc aaatgttcac aaccttggac
    15421 agctagcgca atttctttag gatccaacaa ctcttttaac gtacggatga tccattgacc
    15481 aacttccgca ctggaatttt ttccaatatg tcctccaacg acttcgcttg tgctgcatcc
    15541 taaaacaaaa agatcacctt ttttcaagtg tgcctcagcc aatatgtcat taactatttc
    15601 agtcaattgg tcttttaatt ctttttctga caacttcatt ttctaaaacc ttctttttca
    15661 attaatattg cttccgtgct tgaatacgtt gaagacttgc agttatagga aaggcaagtc
    15721 ccattccaat cagattttgg aagatgtttc ctggtataga agccactcca gccggccaat
    15781 catataaaat ccaaccggcg ataccatagc cgatgatcat aactaagctt cctaataaaa
    15841 gccctaatcc ccacatacga gtagaagatt tgctgaaaga accagcaaca tatccttgta
    15901 acccgtggat cactaatgaa aaaactgccc actgcggata tcctgaaatc aaatcgatca
    15961 atagaccact ggaagcacca acaactaatc cacccgttgg accataaaga aaagcagcgg
    16021 tatagatccc ggcctcgcat aatgtcacaa atccattcgt agccggtaca gggatcaaaa
    16081 ttgttaatga aagggcaact gtcaacgcaa tcagcatagc atagatcgtt aattgcttag
    16141 ttttcatttc tcattcctcc tttttgaata ccgccagacc agacacttcc ggtatgatcg
    16201 ttcatcagca cgccattaga aatacaatgg gcaacaaatt ttttactttc tgctactgct
    16261 ttggtaaaat ctgcccctaa cacaagattc gctgttattg cagaagaaaa ggcacaaccg
    16321 gccccgttta ctgttgtagt gtcgattttt ttatgagaaa atacctcata atgatcaatt
    16381 gaatagagaa gatccacagc ttcatctcca gccatccttg tgccgccttt gatcacaatg
    16441 ttttttgttc ctgaagcagt gagttcttta gcgagatgga tcatttctgt taacgaagag
    16501 ggaagagctt gaccagaaag taaagcagct tctgccaaat taggcgtaat catcgtaaca
    16561 agtggaaaaa gttcttcgat gagctgcgaa atatacaatt gattgatcac agcttcagtt
    16621 tctttgaatg ccaaaacagg gtctaaaatg attgggatat tttgttttgt ttttatgaag
    16681 tctcgaacaa ccatgattgc ttcacttgaa ttcaataacc caattttgat accatctaat
    16741 gaagtcattt tatcaatcga aactaactgt cgtttaagca attccaaaga tatagattcc
    16801 agcataaatc gctcattaat atctagtgcc cctatagaag tcaaggcgct tagtccgaat
    16861 agaccaaaat tctcaaaagt ttttaaatct gtttggattc ctccaccagc aaatggatca
    16921 gaaccgccaa ttgtcaagat tcttttcata acattttcct ccatgaataa gagtataacg
    16981 aaattttcaa aaaagaaaaa cagccaattg gcatattttt atctcatcca atttaaaaaa
    17041 acaaaaagaa aagaaagacc aaaatggatc tttctttcct taaatagtta ggacttttgt
    17101 gattttaatt ctttctcgat ttgaagccgt tgcactttca ttgtagctgt tcgcgggatt
    17161 gcatcaaaat cacggatgat agggacgttc aagcgaggaa gttcatggac ttgctcccac
    17221 catcgattcc aatccatttc tttatcagga gctaaagcaa taatcggttg tggagaatga
    17281 tttttatccc ttacaatcac tacttctgcc aaaaaatcta acgaatctaa taaaaagtct
    17341 tcaatagcta atgtactatt gatcgtttcg atcaaatcca cttgccgatc tttcaaaaat
    17401 aggtggccat gcttatccat aaaaccgtaa tctccgctat cccaccaatc gccataaaca
    17461 ttttcctgga accgtgcatc ttccttgtaa taagtcaaag cccgtccttt agacagtaat
    17521 tgtatatgac catctgtgtt ttcaggtagg acattcccaa ctgaatctgt aatccgagcg
    17581 cttgtcatgt cttccagtcc aacgcccata tctcttgcat cacttgtttt caaactctct
    17641 agtgtatgag ccttaagaat cattggtccg cattcacttt ggccatatac ttgaagaaat
    17701 accgcatcct gtgccatcga cgttcgcaaa aatgtcttca ttgtctggtt gtttatagca
    17761 tcaaaagtgg agtgatagta tcggataccc gaaaaagctt ctggatgctc cttagcagta
    17821 aaacgccact gaacaaagtt attcggatgg gtctctagtg caattggccg gtgagcttcc
    17881 aacatatgaa caacttttga actttgtgcg tttgctaaag gcatcatagg aaatcccatg
    17941 gacatcaaag aagagatacc aatattgaat ctggaatgca ctggagagat gtgaaaagcg
    18001 attaatttct tttccgaaat ttttgtgaaa actgtttttt gccatttcgt ccgccatccc
    18061 atagaatgag cagaatgaca aataagttta gggataccag ttgtaccaga agtatgggtc
    18121 atataagaaa tttcattttt gtctaaaaat gattgagaaa cagacaaagc aggttgttcc
    18181 attaaataaa gaataggaat acccgtggtg ctagttaatt ttaatatcct aaagaatatc
    18241 tcaaagttcc ttcaaaacgt cgttgtgctc gacttaatag aacactgtaa gccagtaata
    18301 tgctttcaaa tctactctct agccctttaa cagaacgtga gttgaaattt tgacaaccta
    18361 atttttctaa ggaagaa
//
LOCUS       DACCXO010000048        18062 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.049, whole genome shotgun sequence.
ACCESSION   DACCXO010000048 DACCXO010000000
VERSION     DACCXO010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.049"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(452..1135)
                     /locus_tag="IVY70_001541"
     CDS             complement(452..1135)
                     /locus_tag="IVY70_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP7958675.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(1153..1770)
                     /locus_tag="IVY70_001542"
     CDS             complement(1153..1770)
                     /locus_tag="IVY70_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP7958676.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(1780..2604)
                     /locus_tag="IVY70_001543"
     CDS             complement(1780..2604)
                     /locus_tag="IVY70_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP7958677.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(2597..3157)
                     /locus_tag="IVY70_001544"
     CDS             complement(2597..3157)
                     /locus_tag="IVY70_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958678.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            3331..4287
                     /locus_tag="IVY70_001545"
     CDS             3331..4287
                     /locus_tag="IVY70_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7958679.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(4592..5548)
                     /locus_tag="IVY70_001546"
     CDS             complement(4592..5548)
                     /locus_tag="IVY70_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP7958680.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            5652..6254
                     /locus_tag="IVY70_001547"
     CDS             5652..6254
                     /locus_tag="IVY70_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7958681.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(6342..7841)
                     /locus_tag="IVY70_001548"
     CDS             complement(6342..7841)
                     /locus_tag="IVY70_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP7958682.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(7857..8381)
                     /locus_tag="IVY70_001549"
     CDS             complement(7857..8381)
                     /locus_tag="IVY70_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958683.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            8679..9428
                     /locus_tag="IVY70_001550"
     CDS             8679..9428
                     /locus_tag="IVY70_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP7958684.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9419..10204
                     /locus_tag="IVY70_001551"
     CDS             9419..10204
                     /locus_tag="IVY70_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7958685.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            10173..10889
                     /locus_tag="IVY70_001552"
     CDS             10173..10889
                     /locus_tag="IVY70_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7958686.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(10991..12010)
                     /locus_tag="IVY70_001553"
     CDS             complement(10991..12010)
                     /locus_tag="IVY70_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP7958687.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(12029..13087)
                     /locus_tag="IVY70_001554"
     CDS             complement(12029..13087)
                     /locus_tag="IVY70_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP7958688.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(13308..14456)
                     /gene="nagA"
                     /locus_tag="IVY70_001555"
     CDS             complement(13308..14456)
                     /gene="nagA"
                     /locus_tag="IVY70_001555"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP7958689.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(14532..15368)
                     /locus_tag="IVY70_001556"
     CDS             complement(14532..15368)
                     /locus_tag="IVY70_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7958690.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(15552..16769)
                     /locus_tag="IVY70_001557"
     CDS             complement(15552..16769)
                     /locus_tag="IVY70_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7958691.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            17152..17235
                     /locus_tag="IVY70_001558"
     tRNA            17152..17235
                     /locus_tag="IVY70_001558"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17186..17188,aa:Leu,seq:gag)
     gene            complement(17475..>18062)
                     /locus_tag="IVY70_001559"
     CDS             complement(17475..>18062)
                     /locus_tag="IVY70_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958692.1"
                     /translation="FLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYF
                     GNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLI
                     VGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMA
                     VKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         5345 a         3737 c         3152 g         5828 t
ORIGIN      
        1 ggatatcaga caactgccca atatccttga catagctaaa taagcatgct tcttcgtgtg
       61 cgctcttccc taaaaaacag aaaaataact agtaattctg tatgaaatta ggtacaatat
      121 acacgtcgat gcacaagcat tgactatggc aacttgattg gtcagttccc gctgactttt
      181 caagccccga caatttagct gctactaaat tgtcgggtgc cttttttagt ttttattcgt
      241 ttttcattct atagaaagcg cttcaataaa tcaagacatt tttttgttag aatgctaata
      301 ataacaaaag gttttaactc ttcttatgta cattacaggt aaaaaaacaa aaaaatttct
      361 cttttttttg aattctgaac aaatagtact ggagtacaaa agacgaggaa gagccactat
      421 ctgctcttcc tcgtcttttg attctcacct gttattcatg attctttttc tcagtttccc
      481 gatatttttc cattttacga ctaaaaagct ctccgctact tggtggcgta tatgtgatgg
      541 catttgctcc tgcttgaatg gtctcagcaa tactttcttc cgttggacca cctgtcgcaa
      601 tgataggaag ttcaggatat tttttacgaa aatggcgtac tgcttctgca gtttctttac
      661 ctgcactgat attgataatc gaaataccag ctgctaactt ttcatctatt tctgtatatt
      721 tcgacgttac cgtactgata actggaatat caactactcg acaaacttct tttactgtct
      781 ctaaaggcgt aggaccgttc aaaacgaccc ctaacgaacc ttgcgcttct gcgaacatgc
      841 tcatgtagga agaacgagca ccttgtgtca aaccaccacc tacaccggag aaaactggaa
      901 tgtcagctgc ctcaataatg cttttagtaa tcgctggatg aggtgtaaaa ggatacacag
      961 caattactgc attcgcattt gtattacgta tgatcgcaat atccgtagta aagatgatcg
     1021 aacggatcaa ttttccaaat atcttgatcc cgcttgcttc taaaatgatt tccggtactc
     1081 ttacgatatc ttttctcaat tccgtcatga tttctggcgt aaattgttct gccatcagcc
     1141 acttctcctt tgctatctcc ggtcatactg attcaattga tacgtctcga tgacctggat
     1201 gattttttgc gcataaccgg ggtctgtcgc ataacctgca tcttgcaatg cttgtgcggc
     1261 ttcctgatag ttggtggccg taatcacttt ttcatacagc gcaggattcc aatctactcc
     1321 ttggacgaac agttgtgtat ggtcatccat ggactgttcc catgaatcat acactttgaa
     1381 gtctccttgg atagttatcc attcttcgtt aacaaattcc tttgtttcca ggcttacttt
     1441 cttctgatcc ccataagcct tgatcccgaa aagattattg tatttgctag ccaaggtact
     1501 ttttccccaa tttgattcta atatagcttg tcctaaaatg atactaggaa gcactccata
     1561 accatcttgt agttcttggg catgcggaac aagccgttca ataaattgct gatgggattg
     1621 cgcttcattt tcttgctgat tatcagtaaa aggagaagac agattcttca aggaaaaaac
     1681 aaaggctaag agtatcactg ctacaccggc aaccactgct ggccaacgga tattcttgcg
     1741 cttttgcttt ttccactttt ttttcgccat gatcaattcc tacttttcat ttaattcatt
     1801 cagatattct tctagcgtca attggtgttt ggtgatatac tcagcgtttt ctttaccgac
     1861 ataacgtaag tgccaaggtt catatgtgat cttagttata tcttcgtgtc cttttggata
     1921 acgaacgata aatccatact gcgcagcatt atctgcgatc catttagctc caggctgatc
     1981 tccgtaactt gcatccaata cagtagaagg ataactgttg taccagtttt gatcgacgac
     2041 atcgactgcc agacctgtgt ggtgttcgct gtatcctggc tccgtaatcg tttctttagt
     2101 aatctttgtc gcctcctctt ctgatacacc ttgacgactc atcacatctt ggacattttg
     2161 ttcgaaaact tgttgttggg atgatacaga ccggtaagct gagaccatca ctaatggaaa
     2221 tccagctgct tcagcagcct tggcaaatgc ttcatattct ggagcaatgc gtttgtcgat
     2281 caagtatccg ttacttaatg atgccaattg agtagactca tcaatttctt tttctaattt
     2341 gtgatctgga ccaaccaaaa ctagcttcca atcgtctgtt ttaacatctg gcaattgttt
     2401 ggacgtacga tcttttgttt ctttgtcctt taccttgctg ctggttgttt gactgttctt
     2461 ttctttacta gcagttgttt tggtgcttgt tcgttcgact gcttgattgc cggcttgaac
     2521 atgcgaatca tcatcgctaa aaataacata gcccattgct gcgatcataa tcacaactgc
     2581 tccaaatcct aaaaatttat tcacttgaaa aatcctttcg ttgttcttca cgttccccat
     2641 tcgcctcatg aacgtaagtt tcttccagat tttcattcac ccaatcgaga agttcgattt
     2701 tttctccttc gatctgtttt tgaacatctg ctggcagacg aaaattcaaa atatcatcat
     2761 agccccattc atcatattga atacttactt tcaagtcaag gtatctacca gcctgctgat
     2821 tatttttcaa agggatcagc tcgacttttg ctgctttgct gctaagccat ttttgcgcga
     2881 tgcgtgtttt gatttgcttg tactctgcaa cggcaacgtt tcgtttttcg ggaaaaataa
     2941 tcgtttgacg caaaatcttt tgcgttagca tccattcata atcagtgaaa agatttttta
     3001 ctttctgcat cgcttcagat ggcagatttt tctcgccatc tttccatttt tgccattgtt
     3061 ctggactgat cagcaagtat tcatctaaaa aatttttttc agtttcaaac tgttctaata
     3121 atacactcat aataagatca atatatactt gatgcacgtt tatcccctcc ttttaactat
     3181 tctacttaag ttttgatcaa aaagcacctc ttaaatcgct gctttcattt tttttaatgt
     3241 ttcaagtcct ctcctcttta ttcttcgtga tttttgttat aataatagac agacaagaaa
     3301 ctcaatccaa ctatgaagga gcgttttcag atgaaaaaat atcaatgggg aatcgttggt
     3361 cttggtacca tcgcacatga gtttgccgaa agcttcaatc aagagacgag cgaactaaca
     3421 gcagttgctt ccagaacttc agaaaaagcg gaaaacttcg ctcaccgtta caatattcca
     3481 aaagcttacg gaagctatca agaaatgtta gacgatgctg aaatcgacat cgtgtacatt
     3541 gctgtaccaa atagacagca tatcgaccat atcttggcag cattggaagc aggcaaacac
     3601 gttctatgtg aaaaagccat cacgatgaat aaaaaagaac tggctgacgc tatgcgctta
     3661 gcagaagaaa agaatttgat cttagcagaa gcgatgacaa tcttcaacat gccactttat
     3721 cagcagttac gctcaataat ggatacagga aaactggggg ctttaaaaat gatccaagca
     3781 cctttcggca gttacaaaga accagaccct aaaaaccggt tcttcaatcc agaattagct
     3841 ggaggagctc tgttggatat cggtacgtat gctgtatctt tcgctcgttt ctttttgagc
     3901 tctcaacctg aagtagttgc ctctacaatg gttccttttg aaacaggcgt ggacgaacaa
     3961 tccgtcacta tcttgcgtaa caaagaaaat gaacttgctg ctgtcagctt gacttttcag
     4021 gcaaaaatgc caaaagtcgg tatcgttgcc tttgagaacg gttatgtgac gattgctgat
     4081 tacccgcgtg ctgatcgtgc ggagatctta tttacagatg gtacaaagga attcatcgaa
     4141 agcgggaata catctgaagc aatgaattac gaaataaaga atatggtaca catgattgaa
     4201 ggtgacttgc ccaatcgctc tctcttcttg acaaaagatg tcatcgaaat cttggatcaa
     4261 atgcaagttc tatggaaaaa tatgtaattt taaaaggttg tgaaaaagct gttctgcatc
     4321 aagtaataag aacagccaaa caaaaatagc tcctcctatt tttcgtttgg ctggacttat
     4381 taccgcagag gaaagctttt gaacaccgtt taagaaagag gttgtgagcc taccgttcag
     4441 ctccgagtca caaggagcaa tttcaaaaaa cagcttctca tgtttgttga atattgtgac
     4501 ttgtgaccga ggagttgaca ggagaacacc gtttaaagaa gaggttgtga aatcagcttt
     4561 ttgacctgaa tattagctga attcacaacc tctatttttg gattttttgt tgggagaatt
     4621 ctgccttgat ttcaataaaa cttttaaaag actgcaaaaa ctggaacaat tctgctcgat
     4681 cctcgaattc agggcgacta gtcggcagag gcatttgccg atacaataca taaacttctt
     4741 caatacgtgt taaaatttgt ctgccatcat ttgactctga aaatgtttct gcagtataaa
     4801 tcaataaacc atagatatgt tttccataat aagctggttg ctggattctc tctagatttt
     4861 ccagcatgtc tttaatcaca ttggcctgtg ctcttctcat agagaagtaa gtttcgtagt
     4921 atagcggctg tctgagccag tagttttctt gatgttctct ggcatcttct tggctttcgc
     4981 gaatgaatgt cagtaaatgt tcgcatttga tctgcacatc ttctttattt tctgataaaa
     5041 gaaattctgc catttgtctc aaaatatttc gaaattcttt ttcgatctgt atttggtttt
     5101 ccttcagctg cttcgtacga tctggcatgt aaacatttgc aagcaaagca aatactaatc
     5161 caatcgccat taatcccgct tcattgccga tcagctgcca agacatgttt tcctccacta
     5221 aataatgagt gaccaaaacc gaatttacta ctatcccttc ggttaatttg aagcgagagg
     5281 caagcgggat gaacaggaat aaaaaaagac tgaagccaaa aatagtatac ccaacagcaa
     5341 aaaaactgaa cagtgctgtt gccgtagcta atataaaggc tgcgattcga ttcatgcccg
     5401 tacgcaatgt agagcgcttg gtatttccta cacttaataa tgcaatgatc cctgcagctg
     5461 gccaatacaa cagcgataag ctacccgctg tgaccatcga aaagaagaca ctgatcactg
     5521 ttttgactgt tcgcattcct atacgcattt acttccccct tttttatttt aattatactt
     5581 gaataatgaa aaactagcta tcagacaaaa gtttaggtat gatagtaaaa agaccaaaaa
     5641 ggagaatctc tatgcaattt ggccgttttc gactaggtat gcgtacgatc aaaagtgcct
     5701 tagctgtttt tttatgtatc ttgttttttc atttgactca acgaggaatc ccgatgattg
     5761 ctgcattatc tgctgttttt tctttgcgtc aggacttgac tacaaccgtt tcttttggaa
     5821 agtctaggat catcggcaat acattaggtg gtacattagc tatcatttat ttttttgtca
     5881 aaagctattt tcacaatgat tttttggtag aattgatctt gcttcctatt ctagttatta
     5941 ttgtgatcgt cgtgtcagat ggtattaaca ataattccgg tatcatttcc gcaatagcta
     6001 cgttgttgct gattgcgttg agcatccctc aaggcgaatc cgctttgtat gctatccaac
     6061 gtgtactaga tacttttata gggacattca ttgcaatcgg tatcaatttc tttcttcgtc
     6121 ctcctgaaac agaaaagaag gaagaactat cagaagattt ggtcgaactc caaaagaaag
     6181 aacgattgct aagaacaaat ttacaagaga tagaagctaa gatccaaaaa caaaagaaaa
     6241 atagcgagga ctaattttct tttgcaataa aataaaaaaa tggagagaag aactttaaaa
     6301 agtcttcctc catttttgat tgaacggtga agtgtactac tttattcttc cttcatcgag
     6361 ttttgttctg cctcttttgg cgcttcatac atatttactt ggctagtagt cccacgtgtc
     6421 gacaaattct ctacgatttc cttcaagtca agtcctgttg tcgcttttag tgtttcttga
     6481 gtagaagaaa gcaaatttgt ggcataattt gtcactcggt tcgctccact ggtttctcct
     6541 gaactgcttc ctgtatcgac aacagagatt ttttcgatat ttcctagcgg ctgtgctgct
     6601 tcgcgcatca attgcggtag catatcgata accatactca agacagctgc ttcgccatat
     6661 tccttgaagg cattggcgat cttttgtttt gcttcggctt ctgcttttcc ttttgctagt
     6721 gctgcttcgg cttctgcttg tccagctaaa cgtgtcttat tggcttccgc ttctgccagt
     6781 gcttccacac ggaatcgttc tgcttcggct tcagtcactt ctttgacttt ttgagcttgt
     6841 gcttcttgtt ctttagcgta ccgatcggca tctgcttttt tcttcacttc cgaatcatat
     6901 tgcttttcac gacgtgtgat ttctttttct tccagttcga tttgtttttg acgttctacg
     6961 attttaatct gcatttcctg ttcgattact tgttgttgtg ctcgggcact ttctaaattg
     7021 taggcttggt ctgcttttgc tttagcaaca tcttgttctt gtttgtaggt tgctaatttc
     7081 agttcttttt cttttaatga ttctgcgatc tctgtttgtc gctgtaattc agctgcttgt
     7141 gattcttttt ctgcttgagc ttttttgata cgcgtttctt ttagagcttc cgcttctgct
     7201 atatctgcat ctcttttgac ttgggcgatt cttggtttcc ctaaagagtc aagataaccg
     7261 tttttgtctc tgacctcttt gatagtgaaa gaaacgatga tcaaccccat ttttgcaagg
     7321 tctacagaag cgacttcttg tacgctttga ctgaacttat cgcgattttg atagatttct
     7381 tctacagtca tagaaccaag aatcgagcgc aagtgtcctt ctaatacttc tcttgcttca
     7441 ttttccagtt cttctcttgt cttgcctaaa aactgttctg ctgctgtagc aatctcttct
     7501 acagatgaac cgattttgat gatcgaagta ccatcacaca tcaccggtac gccttgttcc
     7561 gtatacactt caggggtgga gacgtccaat ttactcgata atagactgat ccgattcgaa
     7621 cgttgaaaaa ctggcaagac gaatgcgcca cctccacgca caattttgat tttgttattg
     7681 ctttcatcaa tgtgcacatt tttgcttcct aaataacttc cactgatgat caacgcttca
     7741 tccggttttg ctgtctggta cttcgctaca aagacgatca gcaacatcag taaaatcaaa
     7801 gctgcgattg cgatcgcgat aaaaacaact ggtatttcca tttaacacac gctcctttaa
     7861 gcttttatca tgctttcata aggcatgaca taacagattc tttccttgat ctcaatgatc
     7921 aatactttcg ttccccgctc agctgtctgt tcttgggggc tgtaaaaaca agctggacga
     7981 ctaatggaac cggtaacact tttcaactga atttctccca tacctcgtac agggatcggt
     8041 gtgatcacaa cagctacttg tccttccaga gttttttcag aagacgacat cgttgattcg
     8101 gcatgtttca tcggcatcaa tatatagaaa ttaagcaaaa ataccaacac tatcgatatg
     8161 cctccactag caataaataa aaataaagat gattgatctg ttaaacgctc acctagaaaa
     8221 ccaaataagc ttgtgaaagt taaccaaggg ataataagca tcggatcaag cgggccatca
     8281 aaatcgaata catctccaaa aataatcaat aggactgcca atcctgcaca aatcagcaat
     8341 gtataccaat aaatcgtctc aagtgcgatt ccccctatca tatacacgcc tccttccatt
     8401 tctacgtcta tcataccaat gtttgaaaat tctgtatagc ttcttttacc gactcagtgg
     8461 tgttttttat aggaaggttg tgtttatcgt tgacaatttg ttaaattttt gttttcatgt
     8521 taaaattttc acaataaatt ctttcgaggt agcctatgtt tccttttttt aagaaaaaac
     8581 cattaaagct ccagaacacg atcatccttg tcgtttgttt ctccacattc gtttctttga
     8641 ttgtttctgc catattgatc aggaatttta ttctttcgat gaaccgatct agtgtgtcag
     8701 ataaaatctc tagcattgct aaaatcgttg ccaatgataa actcatccaa aaaaagctgc
     8761 agactggtga cccgacacat gcgattcaag gaataacaaa tgaaatcatg attgcatcga
     8821 atttagattt tatcgttgtc atggaccctg aaggcattcg atattctcac gcagacccaa
     8881 gttacatcgg tcttcctttc tcaagtcatg aagatgtgaa aaaagtgatt ggaggacaag
     8941 aatttttctc tgaaaaagaa ggaatgttag ggttcggctt ccgctatttc gtccctattt
     9001 attatgatca agagattatt ggtgcagttt gtgttggctt aacaaacaac acgattgaca
     9061 atcaagtcaa agatggacaa aaaatgattg ttttttcatt gattattgga cttagcgtag
     9121 gcatcatagg cgccgttttt cttgcccaaa aaatgaaaaa agtactgctt ggacttgaac
     9181 cacaagaaat agcagaacag ttaagtgaaa aaacgattat cgaaaatgaa gtagatgaag
     9241 gaatcttagc catctcttct gataaaacta tcctcttgat caatacacct gcaaaaaaca
     9301 ttttctacca aacagagcag ctattggatt tcgaagtagg aaaaaaatca gtgataatct
     9361 atatgacgtt ttgtttaaag agacgattga gcatcaaacg accgttcgga ctcaatccat
     9421 gtatttaaat gggatagaaa ctatttctag cgttacacct ctttttagca atgaccgttt
     9481 tttaggagca gtcgcaactt tacgaaatcg atcagaagta atgaaattaa tggaggagct
     9541 tggggggacg aaacaatata taaactccat gagagctcaa acccatgaat tcatgaataa
     9601 acttcatgtc atttctggtt tgattgattt gaagaagtat gaagaagtca cccattttat
     9661 ccagcagcta aatcaaaatt ataaaaaaga gattggccaa gtctcaagtc tgatcaaagt
     9721 acctgcaatt gccggttttt tattaggcaa aataaacgaa gccaaagaac aatctgttga
     9781 aatgctgtta gaaagcgatt cttttatccc taaaatggag atggatgact ccatccattc
     9841 acttttgcaa gtgttaggga acttacttga taatgcaaaa gaagccgtgc tacaaaaaca
     9901 tgaaagaaac ggacaggtcc ggattcattt aaactatgac acagaagctg aagtattcat
     9961 cgtgaccgtc tgcgatgacg gtgtcgggat atcagaagaa atctcagaaa aaatatttac
    10021 gctaggtttt tcgacgaagg gaatcaatcg tgggtttgga ttgaatctca tccattcaat
    10081 cgtcaaacgc cacggtggat ttttagaatt ttccaaacaa acaaatggcg gtacaatcgt
    10141 ctatctagaa ttaccaaaaa aggaagatca aaatgatgac aaatgtcctg ataatcgaag
    10201 atgatccaat ggttgcagaa ttgaatcgta cttttgtgga aagcatccct aatatgaacg
    10261 taatcgaaag tttcagaaat gtagaagaag gaaaaaaata tttcagacaa aaaaacgcag
    10321 atctgctgct gcttgatgta tacatgcctg gcgaaagtgg tatccatttt ttatcttggc
    10381 ttagacagca aggaaatgat atcccagtga ttttgatcac tgctgcagat gatatcgaaa
    10441 ccataaagaa tgtgatccgc tacggtgcct tagattattt gatcaaaccg ttcactttgc
    10501 aacgttttca gctggcgatc gaaaagtatc ttgctttctc tcagacaatc caaacgactg
    10561 aaaaagccga tcagaaattg atcgatcaag tattcaacca caccactccc attcacgaaa
    10621 taccatcatt catcgatttg cctaaaggtc tgtcaaaact gactttaaaa aaaataagtg
    10681 catttattct aaaagaagat actatttttt caacaaaaga actggctcaa aaaagctcac
    10741 tgtcacgtat atccgtcaag aaatatttga attttttagt agaacaagat atattgaacg
    10801 aaagaatcaa atatttggaa atcggccggc cgctaacctg ttatgaattg acaaagaatg
    10861 ggcggaaaaa gctcacagat tactgttgag tcatcgaatt atcccataca aaatagtcca
    10921 agtatacaga agtaacaact gtttacttgg actattttta aacattttat tgacgaatat
    10981 ccatgttttc ttatcttaat tgacgggctt taggaagaga tacctcattt ttcgtttttc
    11041 cagtttctat tatttcacga aagttctcga aacttgtcga aatcatattt gataatgcct
    11101 catctgtatt tgaacctaca tgcggggtta caagaactct agggtacatt tctactaatt
    11161 gctggatagc agggtctgga atcgtctccc atggcttgaa ggatcggaaa aaaacctctg
    11221 tttcatttgc aaaaacatcg gttgcaaaac cagctaaatg attgcttttc aaagctctta
    11281 aaatagcttc attatcttga agctctccac gggctgtatt aatcaagatg ctaccttttt
    11341 tcatttgagc taaaaatcct tcattgatca tttgatcgtt ttcacctggg atatgaggta
    11401 catgaatact gacaatatcg cttttggcta ataactcttc aagcgagtgg aacgtcaata
    11461 cttttttagc agcttctgtc tgatgaatat cgtagccgat cactttggct cctagtccct
    11521 taaacaaaac agcttccgtc aaccctatcc tgcctgttcc tatgatccct actgtgcaat
    11581 ttctgatttc acgactaaac atttgctcat ccactcgaaa atctttgaaa gatgttcgga
    11641 tcgtcgtata cgcagtattt ctcaaaagca tcatggcaaa agtcaaagat aactctgcta
    11701 tcgcatttgg cgaatacgat ggtacacggg ctactgtcat accaaaatca gctgccgctt
    11761 gtagatcgat atgattgact cccacagtcc gtgtaaaaac ataacgaatc ccatattcct
    11821 gcatttttgc aagattggtc tcatctgcta tacaatttcc cctcaacaat acagcatcat
    11881 ggtcctttgc tgacacgata ttgtcatgtg tcaacagttc ttcataaaga ctgagatcat
    11941 aatggtaaat attcaatttt tcaaaaaaat ctatctcgtt cggacgaaca ccgtaacatg
    12001 cgattctcat actttcttcc tccatttctt atgaaaataa acctatgcca tgaatcattt
    12061 gtaatacaac gatccataca ggtgtcagta ccacagcaaa aatagttgac agtaaggatg
    12121 cattggaggc taaaagtgcc tccctatcaa aactgatagc ataggctgct gccactgttg
    12181 cagctggtgt agccatcatg ataacgattg ttgccagtgc ttcaaaattg actggtagaa
    12241 tattcgttgc cgtcaaaact gctagaatca cgatattcaa tgcaggtaca aataccactt
    12301 tgactagact gtaataccat gaagtttttt cagatgcagc ttttttgaag cttacttcac
    12361 ctaatgttgc gccgattgct agccacgcca atggtgaact taaaccagct aaataatcca
    12421 tcggctgaaa tagccacggt gcagtctggt caattcgcaa aaacgctact tggcttatct
    12481 ctccttctgc acctgtaact gacacttgtg gcagataccc ttggaacagc caaatgaaaa
    12541 ggccggcaaa agtagcgata atgattggat tgacgaacat cgctttgata tttttagctt
    12601 ccatcttcaa gccactcatt tttatgtatc cataagaata caaaaagatt cggtagccaa
    12661 tattgaaaat tgaggaatac atcacgccga caggaccata aatcgcactg acgataggcg
    12721 ttccgaaaaa ggtcgtagaa ccaaagatcg tcaggacacg caacgttgtt tgcttatccc
    12781 ctttatagct taagaaaaaa ggttttgaaa ctagaataag aataatataa ataactaatc
    12841 cccaaatcaa gacattgatt ccttgcatca gtaattctgt attgatatct tgcatgaaag
    12901 ctcgaaatgc cagtgctggc aaagccactg ttaagacgac tttcgataga atttttccaa
    12961 cagattcaga aaaaatcccc ttttttctac aaacaaaccc taataatata ataaaaactg
    13021 ttgaagtgat tgcactgaca atcgacatat ctgtcaaagt cgatcgaaaa atgtcaaata
    13081 agttcatctt tctcctccta attatgttca tttattcaca ataaaaaaag cttttatttt
    13141 gctaatacgt attaacattt ttaattttat ctttacattg ttacttattg aacaatagga
    13201 aattaaagaa acttttgaaa cttatgtaaa gtgattttta ctagaacaca aaaaaaacct
    13261 taaaaccaaa cttaagaagt ttagttttaa ggttaactta aattatatta tgcgtggaaa
    13321 cgttccacgc catctagata agttgcttcc aatgtcatat cagggttcaa tacgataaag
    13381 tctgcatcgc gtccttgagc gatttttcca catgtatcat cgattttaca gctgattgct
    13441 ggaatcagac tagccatcat gactgcttgt tctggtgttg ctaatcccca gtctacaaca
    13501 tttttaattg cttctttcaa tttcaatata cttccagcta agttgccaga ttctagacgt
    13561 gctgttcctt cttttactac tactggaaat tcgcctaaat tgtaatttcc atcaggcatc
    13621 ccaccggcca tcatacagtc tgtaattaat gcgacatgat catgtcctgc tttttccatt
    13681 aggattcccg cagcatttgg atgtacatga tggccatcac aaatcaattc agaaaagaca
    13741 tgttctaaag ataataacgc acccaccata cctggttcgc gatgattcaa tccacgcata
    13801 ccattgaaag catggacaaa tacacttgcg cctgcttcaa cagctttttg cgcttcttct
    13861 agtgtagcat tactgtgtcc tagagaaaca actactcctt cttcagtcac ttgtttgaca
    13921 aactcttcta caccttgacg ttccggtgct aaagcaattt ttttgattaa accacctgaa
    13981 gcttcttgcc attcatggaa agtatccaaa tcaggatccc cgaaataact tgggttttgt
    14041 gcccctttgt gttcttctgt gaagaaagga ccttcaaaat agattccttt gattttagct
    14101 cctggtgctt cctgatacat tttgccgatt gtctcagcta catcttttaa acgttctttg
    14161 cttgacgtca atgttgttgg taaaaatgaa gtcactccac aagataacag accttcagac
    14221 atcactttga tgccttctgc atcattatcc attacatcat gattcatata gccgtgaata
    14281 tgtgtatcta ccaaaccagg agcaatccat ttgccgcttt ggtcaatgat gttgacttct
    14341 ccctcaggta gttctttggt gtattcacca aaaagtccat cggtaatttc aagatagcct
    14401 gcatgtttga cacctgacgc taaaaagaat ttgtcagcat agataaaagt tctcattcta
    14461 ttctctccct ttgtttttct acctctaaag tactcttatt tagagaagaa gtcaagaatg
    14521 gtctacacca tttacaaatt ttttgaaatt tgtttcacag cttgtgctaa gccttcatag
    14581 aatgctgctc tttctggttc ttctttacgc ttgtatatta agcttttaag cacaaataaa
    14641 tcattgaggt aatagtagct attcttctct tgagcccaat agattccttg attcacctgt
    14701 tctaacgctt cgtcgatttc attttgatgg aataagaaat ttccataatt ataaaaaatt
    14761 tgcgaaaatt cactttttga tcgttcatca gctgattgat gcagcagttg gatgcttcga
    14821 ctcaaatagt agcgtgcttc agccgttttt cctgccacac catatatttt tccgatacaa
    14881 ctaatcagtt gtatttctgt gtcagaaaca tggattcttt ctttttggct agagtagctt
    14941 aatgcttctt tcaaataata aagggactga gagatatttt gacgcaacgt gaattcgcaa
    15001 acaccgagat aataataata gcgttgaaag tcagttgctt gatacagatt ttcagaaata
    15061 tcagaagatt ttagtacttg ttctaacttc cgataatcac gtttttggaa acaactatcc
    15121 aacagttcaa ataatttatc gtttgtccga gtagtttttc gagagagatt catcacatga
    15181 tcaatcgtta cccctagccg ttgacaaatc tgcatcatca ccaatacatt cggcagttcc
    15241 tcattatttt cgatccgact taatacactt tgtgaacaaa tatcatgcga aagcattttt
    15301 tgcgtcattt tttgttcttt tcgaatctct ttaatgatcg ttccaaagga ctcgattaac
    15361 gtttccataa gtatcctccc caaaatatgc gtattcgcat tctattcgta ttttacttta
    15421 ggaagtcgca aacttcaaga aaagacttgc aactaaataa tatactttag ttatttaaga
    15481 aaactccact agaaagagag cctcagtgtg tttgtctgtg gccctcctac tgaaaaattt
    15541 ggtttttaaa atcatttttg gcgataggtt gctaggaatt ctctcatttt cttagctgtt
    15601 tgtttcaaat catccatttt aggaagataa acgattcgaa aatgatctgg ctggttccaa
    15661 ttaaatccac caccatgtac aagtagaata tgatgttcat gtaaaaagtc caaaacgaat
    15721 ttttcatcat ccaaaatatt gaatcgcttt gtatcaattt ttgggaatat ataaaaagca
    15781 gcttttggtt ttacggctga aagaccagga atatcattga ttgcattata gatatattct
    15841 ctttgttcat agatccttcc gcctggcagg agcagctcat cgacactttg ataaccgcct
    15901 agcgcggttt ggataatttg ctgagacaat acattggaac acaaacgcat ggaggacaac
    15961 atattcagcc cttctatata gcctttgacg tgtgacttat ctccacttag gaccatccag
    16021 cctactcgaa atcctgctac acgatgagat ttggatagac cgttcagcgt aacaacgaac
    16081 aaatcaggcg ctaatgtagc aatcggaata tgttctagac catccatcac taagcgatca
    16141 tagatttcgt cggaataaat aatcaggtta ttttgtcgag ctacttcaac gatttcttct
    16201 aataattctt ttggataaag tgccccagta ggattattag gattaatgat aacaatagct
    16261 tttgtccgac tcgttatttt tgatttgata tcatcaatat caggatacca ttctgcctct
    16321 tcatcgcaaa tataatgaac aggtgtgcct ccagctaatg caatagaagc cgtccacaac
    16381 ggatagtccg gcattggaac cagtacttca tctccattgt ccagcaaccc ctgcatacac
    16441 atcgtgatca attcgctcac tccgttgccg gtgtagatat cattgatcgt cacattcgga
    16501 aaatgtttca gttggcaata ttgctcaatt gcttttctag cagaaaagat ccccttggaa
    16561 tctgagtagc cttctgagtc acgggcattc atgatcaaat cacgaatgac ttcgtttggt
    16621 gcttcaaatc caaaaggtgc gggatttccc gtgttcaatt ttaaaatact aatgccctct
    16681 tcttgcatcc gctccgcttc ttctagaacg ggaccgcgaa catcgtaact gacaccttct
    16741 aatttgttgg atttttcaaa ttctctcatc acttagccaa tccctcattt caaaaagtta
    16801 gaactttcca aattttttgt tatttactat ttcaatatca tactccatcc tagaaaattt
    16861 tgccacttat ttaggaaaaa cgtaagtatt aaaaaaacac aaacaagcgt tgagaactta
    16921 attgtcatga tagtcattcc atagattcgc tgcccaatcc attgcaattc cacaaatgat
    16981 tcctagcata aaaccaagca aaaaacgtcc gaatgcaagt ccaatcaata cacctaagat
    17041 cattcccagt cctgaaaatt ctccgtcata tttctccatc ttaataagtc ctcccatttt
    17101 tgagcttgac aactatgtaa aacctcgcta tcattagtgt gttattatta cgcagatgtg
    17161 ttggaattgg cagacaagca tgattgaggg tcatgtgagc gttagctcgt gtgggttcga
    17221 gtcccatcat ctgcactaca actcgtgaag ttaattttgc ttcacgagtt tttttgtttg
    17281 aacgtttctt tatgccatta tatacatatc actgtatcct ttacaaaaag aacgtacaat
    17341 catgtaaaca gcctgaggtg agtatatgaa ggtcggaaac aagctactcg tagaaacttg
    17401 caagtgttaa aacaattaat cgaaaaacga gtaaaatccc tcacctaata ctaaagcgag
    17461 ggattttttg tatattatct gtttatcacc gttattataa aggtacaaat accaagcacg
    17521 gttaatccaa attgcataaa ttcatttttt tgtacactca tgcttttcat tagctcttta
    17581 actgccatta gtactaagat ggcgataaca acataaagta tatagtacgt ccgcattccg
    17641 gaagcccata ctgtgggaga aaatcccata attaaccttg aacaaaatcc cattatcatc
    17701 aaaataacta aaaaaataga agttttctta ttgtcaaaac ttaaataaat tccaacaatt
    17761 aaacatagta ataatgccaa gactactaaa gtagctatcc aagtacctgg ataaaaaagt
    17821 gacagtttcg ttcctaactc tgtaaagaga ttattcaggt tactactact ccaaatcatt
    17881 gcccttttat ttccattgac gtaagtaaag ctttgaccca ttgtattacc aaaataaata
    17941 atcaggttta agaaaaacgg taatgcagtc aatatacgca cataatagtt ttgacatttc
    18001 ctatatgtta gaaggaaaat taagaaaaat agcaataaaa ataagatatt catgtctaaa
    18061 aa
//
LOCUS       DACCXO010000049        17760 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.050, whole genome shotgun sequence.
ACCESSION   DACCXO010000049 DACCXO010000000
VERSION     DACCXO010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.050"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            49..861
                     /locus_tag="IVY70_001560"
     CDS             49..861
                     /locus_tag="IVY70_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP7958693.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            858..1658
                     /locus_tag="IVY70_001561"
     CDS             858..1658
                     /locus_tag="IVY70_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP7958694.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1819..3087
                     /gene="rseP"
                     /locus_tag="IVY70_001562"
     CDS             1819..3087
                     /gene="rseP"
                     /locus_tag="IVY70_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP7958695.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3155..4864
                     /locus_tag="IVY70_001563"
     CDS             3155..4864
                     /locus_tag="IVY70_001563"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP7958696.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5076..9428
                     /locus_tag="IVY70_001564"
     CDS             5076..9428
                     /locus_tag="IVY70_001564"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP7958697.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9572..10045
                     /gene="rimP"
                     /locus_tag="IVY70_001565"
     CDS             9572..10045
                     /gene="rimP"
                     /locus_tag="IVY70_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP7958698.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10069..11244
                     /gene="nusA"
                     /locus_tag="IVY70_001566"
     CDS             10069..11244
                     /gene="nusA"
                     /locus_tag="IVY70_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP7958699.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11266..11559
                     /locus_tag="IVY70_001567"
     CDS             11266..11559
                     /locus_tag="IVY70_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP7958700.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11556..11867
                     /locus_tag="IVY70_001568"
     CDS             11556..11867
                     /locus_tag="IVY70_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP7958701.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11880..14186
                     /gene="infB"
                     /locus_tag="IVY70_001569"
     CDS             11880..14186
                     /gene="infB"
                     /locus_tag="IVY70_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP7958702.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14213..14560
                     /gene="rbfA"
                     /locus_tag="IVY70_001570"
     CDS             14213..14560
                     /gene="rbfA"
                     /locus_tag="IVY70_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP7958703.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14668..14889)
                     /locus_tag="IVY70_001571"
     CDS             complement(14668..14889)
                     /locus_tag="IVY70_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7958704.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15337..17700
                     /locus_tag="IVY70_001572"
     CDS             15337..17700
                     /locus_tag="IVY70_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAP7958705.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6056 a         3149 c         3912 g         4643 t
ORIGIN      
        1 tataatactt aagatagtgg gaatctttta agtataggag gaaaaagaat gttacgtttt
       61 tttccgcaaa aaaacaaata tattcaagaa aaaagcgaat ttcaatttaa taccgatgga
      121 ccaattcctc agcacattgc catcatcatg gacggcaatg gtcgttgggc gcaaaaccgt
      181 cgtttgccac gagttgctgg ccataaagaa ggcatggaaa cagtaaagaa agtcacaaaa
      241 aaagcatcaa ggcttggagt gaaagtgttg acgctttatg ccttttcgac tgaaaactgg
      301 aagcgaccaa aagatgaagt aagttttctc atgcaactac ctgtcgactt ctttgatacg
      361 ttcgtaccag aattgattaa agaaaatgtc aaagttcatg tcatgggtta tgaaaacgta
      421 ttgccagaac atacgcaaga tgctgtaaga cgtgcaattg agcaaacaaa aaacaatact
      481 ggaatggtat tgaattttgc tttgaattat ggaagtagag cagagatcgt gactgctgtc
      541 caagaaattg ccgaagaagt agcaaaagga gaaatacacg cagaagaaat cgatgatgaa
      601 ttgattgcta agcatttaat gacaggtttc ttgcctaaag agttacaaga tcctgagctg
      661 atgatccgta caagtggaga agagcgaatt agtaattttc tgttatggca gatcgcatat
      721 agtgagctgt attttacaaa agcgctttgg cctgattttg atggtgcgca tcttgaagaa
      781 gcaatcgcct cttatcaaaa tcgagaccgt cgattcggcg gtgtaaaaaa aacagaagat
      841 gagaaagggg atcagtcatg aaacaacgtg tgatcacagc agctgtggcg ctagtcttgt
      901 ttattccgat tatatggatc ggaggcatag cggttgagct tacagcagca atcttagcgg
      961 tagtaggagt ttacgagctg ttccggatga aaggattgac actcttaagt tttgaaggag
     1021 ttttatccgc cgttggtgca gtgttacttg ttttgccaaa agaaagatgg tttttctttc
     1081 ttccagagaa tgtaagtaca tttatgttat tttatcttat agttatgatt cttttaggaa
     1141 catcagttat ttctaaaaat acgtatacga ttgatgaagc aggttttcct gtgcttgtca
     1201 gcttgtatgt gggaataggt ttccagaatt ttgtcaatgc acgaacagaa agcttggctg
     1261 ttttgatatt tggtctattt attgtttggg caactgatat cggagcatat atggttggtc
     1321 gacgttatgg tcagcataag ttaagtcctg atatttcgcc caacaaaacg attgaaggtg
     1381 cgctcggtgg tatcggtagt gctgttgtag tcgcatttct ttatttattg ctatacccag
     1441 caaaagaact attcaatcat ggtatgtttg tcatgcttct gatgacgatt attttttcag
     1501 cagtcggaca atttggtgat ttggtcgaat ctgcaatcaa acgtcattat gatgtgaaag
     1561 actcgggaaa tatcttacca ggccatggtg gaatcttaga ccgtttcgac agtttgctgt
     1621 ttgtatttcc tatcatgcat ttatttggca ttatttagta agtatacctg cgtttctttg
     1681 tgaatttcat aaaagacgca ggtttttatt tttcattata ttgatactat tggtgtcttt
     1741 ggtatactat ggtaaaatta tagtgtttta tcgttgtttt ataaaaaaca caaaaaacaa
     1801 gtaaaggaga aaaaagctat gaaaacgatt ctgacattta tcatcgtttt tggtatatta
     1861 gtgattgttc atgagtttgg tcatttcttc tttgcgaaac gatcaggaat cctcgtccgc
     1921 gaatttgcta tcggtatggg tccgaaaatt tatgggcatc aagcaaaaga cgggacaaca
     1981 tatacgcttc gcctattacc tattggcggt tatgttcgaa tggcaggcaa tggggatgat
     2041 gaaacagaaa tggcaccagg aatgcctctt tctcttttac taaattctga cggcatcgta
     2101 gagaagataa atttaagtaa gaaaatccaa ttgacaaatg ctatcccgat ggaattgagc
     2161 cgttatgatt tagaagatga gctgactatc actggttatg tgaatggaga cgaaacagaa
     2221 gtagtaacct accctgttga tcacgatgcg acgattattg aaaacgacgg aacagaaatc
     2281 agaattgcgc caaaagacgt acagttccaa tcggctaaat tgtggcagcg tatgctgacc
     2341 aactttgctg gacccatgaa taactttatt ttggcaatcg tgttattcat cattctggca
     2401 tttatgcaag gtggtgttca agtgacgaac actaatcgcg taggagagat catgcctaat
     2461 ggagctgcgg cagaagcagg cttaaaagaa aatgatgagg tagtcagtgt ggacggaaaa
     2521 gaaatccatt catggaatga tctgacaaca gtcattacga aaaatccagg taaaacatta
     2581 gactttaaga tcgaacgaga aggtcaggtg caatctgtcg acgtgacacc gaaatctgta
     2641 gagtcaaatg gcgaaaaagt tggccaactt ggtataaaag ccccaatgaa tacaggtttt
     2701 atggataaga tcattggagg gacgcgtcaa gctttcagcg gttcattgga aattttcaaa
     2761 gccttaggat cactatttac aggtttcagt ttagataaat taggcggacc tgtcatgatg
     2821 taccaattgt cttcagaagc agcaaatcaa ggtgtaacaa ctgttattag tttgatggcg
     2881 cttctttcaa tgaatctcgg aatcgtcaat ctgcttccga ttcctgcctt agatggcggg
     2941 aaattagtct tgaatatttt tgaagggatt cgcgggaaac cattaagtca agaaaaagaa
     3001 ggaatattga cattagctgg ctttggattt ttaatgttgt tgatggtgct agttacatgg
     3061 aacgatattc aacgattctt tttctagaaa aagttgacaa atagggtata ataacaaacg
     3121 ataactattt tgaggaaata aaggagtata tagaatgaga caatcaaaaa tgttgattcc
     3181 aacattacgc gaagttccaa atgatgcaga agtattaagc catcaaatct tgctaagagc
     3241 tggatatatc cgccaagtat cagcgggtat ttattcatat ctgccattag caaatcgtgt
     3301 attggaaaaa ctgaaacgca ttatgcggga agaatttgaa aaaatcggag cggtagaaat
     3361 gctgatgcct gccatcttgc cagcagaact ttgggaagaa tccgggcgtt acgaaacata
     3421 tggaccaaac ttgtatcgtt tcaaagaccg caatgatcga aaaatgatcc ttggaccaac
     3481 tcacgaagaa acttttactg atttgatgcg taatgaaatc aattcatata agaaattacc
     3541 tttaaatctt tatcaaatcc aacctaaata tcgtgacgaa aaacgacctc gcttcggttt
     3601 gcttcgcgga agagagttta tcatgaaaga tgcatattct ttccatgcaa cggaagaaag
     3661 tttggacgag acatataaag attacgaaaa agcctatacc gaagtattca agcgttgtgg
     3721 cttagaattt cgctcgatca tcggagacgg cggcgcaatg ggcgggaaag actcaaagga
     3781 attcatggcg atctcagaaa tcggggagga tacgatctgt tattcgactg aaagtgatta
     3841 tgctgcaaac ttggagatgg ctacaagcta ctatgtatca aaaaaatcgc atgaaacaca
     3901 gcttgaattg gagaaagtag ctacacctaa cgtaaaatca attgacgaag tagcagattt
     3961 cttttctgtt gaacctcaaa aaatcatcaa gtctgtattg ttcattgcgg atgaacaacc
     4021 tgtccttgtt ttggtacgtg gagaccatga agtcaatgac gtaaaactga aaaacttttt
     4081 atctgctgac tttttagaag aagcaacaga agaagaagca caaaaatatc taggtgctga
     4141 gtttggttct gtcggaccag tcggtgtttc agatgacgta aaagtatatg cagaccgtta
     4201 tgtccaagat ttagcgaatg ccattactgg agcgaacgaa acaggattcc actataaaaa
     4261 tgtgaatcca aaacgcgatt tcactgtgct ttcttatgaa gatcttcgtt ttgtacaaga
     4321 aggagatcct tcaccagaca acaacggtgt tcttgccttt actcgaggaa tcgaaatcgg
     4381 acatatattc aaaatcggca ctcgttacag tgaatctatg ggagcaactg tactagacga
     4441 aaatggacga gaaaaatttg tgattatggg ctgttatggt atcggtgtaa gtcgtttatt
     4501 atcagcaatc gtagaacaac attcagatga acaaggaatc aattggccaa aaggaattgc
     4561 gccatttgat ctgcatatcg tccaaatgaa tctaaaagac gaataccaaa caagcctgac
     4621 gaatgaacta gaagaagcga tgacaaaagc tggctatgaa gtactggttg atgacagaaa
     4681 tgaacgcgca ggagtgaaat tcgcggattc tgatttgatt ggctgtccaa tcagaatcac
     4741 tgtcggtaaa aaagcagtgg atggaatcgt agaagtgaaa atcaagaaaa ctggcgaaat
     4801 ggttgaagta cgtaaagaag aattggcgaa tactttacca atcctcttga accaaggcga
     4861 ataataagcg ttaaaacaca gaaaataaaa atagctgaat cagctgtttt tgacaatctg
     4921 atgattcgct tttaaaattg gaactgcgat ttgaatcgca gttccaattt tttaatgtaa
     4981 aactcagaaa aaacgagtat actaaaaggt actgaaaaag aagaggataa gatgcgatca
     5041 attgcattgt ttctcatcag aaagggcgta tatctttgtc agaaaaacga gatttatttg
     5101 aaaaactgtt ggaacaaatc caactagagg aagcagaaaa aaatcatcca ctgcttactg
     5161 ctggtgaaat ggatagtgta gtggtccatc gtaaatcgcg tttatgggaa tttacgctac
     5221 atttttccaa aattttgcct attatgctat atcggtcatt gactcagcat ctgactatcg
     5281 cttttaaaga tattgcacaa gtcaaactga atatcttagc agatgaccag acattcgatg
     5341 aacagctctt gcaagattac tgggcccaag ctttggaaaa tcagcaatgc gatacaccac
     5401 tagcacagca agtattgaaa acgcaggtac ctgtgataaa agatcgtaaa gtcatcttgc
     5461 caatcgacag tacaggagct atttcttatt tgaagcaaca atacttgcca ttagtagaag
     5521 agttgttcgt cagttacgga ttcccaaaat ttcgtatcga accagaagtg gatgagcagc
     5581 aagcggaacg tgtattgaaa ctttttgaag aacgcaaaca agaacaggca gaagccttta
     5641 tgaagcaagc agctgaaagt ctagttgtcc acgaacaaaa gaaaaaggaa agaaaagagc
     5701 aatcgccagc tcttgaagga cccatccagc taggacgaaa tattccagcc gatgaaccaa
     5761 cgacaccaat gatcagtatc gttgaagaag aacgaagagt cacacttgag gggtacgtat
     5821 tcgacaaaga agtcagagaa ctacgttcaa aacgcaagat cttgactttg aaaatcacag
     5881 attatacttc atcatttatc gtcaaaaaat tctcgaacgg cgaaaaagat gaacaagtat
     5941 ttgatgcgat cagtgtcggt agctggttaa aggtacgagg aagtattcaa gaagatacgt
     6001 ttgtccgtga tttggtcatg aatgcgcaag atatcataga agtcaaacat acacctcgca
     6061 aagattatgc tcctgaaggg gaaaaacgag tagagcttca tgttcatagc aatatgagta
     6121 cgatggatgc aacaaacagc atctctgatc ttgtggcaca agcaggaaaa tggggacacc
     6181 gagctatcgc cattacagac catggtggtg cgcaagcctt tccggaagca catagtgctg
     6241 gaaaaaaagc aggtgtgaaa atcttatacg gcgtggaagc gaatgtcgta gatgatggtg
     6301 tcccaattgc ttataatgat gcacatgaag cattgagtga agccacatac gtcgtatttg
     6361 acgtggaaac gacaggattg tctgcagtat atgacacgat catcgagcta gccgcagtca
     6421 aaatgtataa aggaaatgtg atcgaaagct ttgatgaatt tatcgatcca ggacatccat
     6481 tgtcacgaac aacggtagat ctgactggta tcacggatga aatggttcga gggtcaaagt
     6541 cggaagaaga agtattgcgg atgttccttg aattttcaaa agatacaatt ttggtagccc
     6601 acaacgcggc ttttgatatg gggttcttga atactagcta cgcaaggtat ggtattcctg
     6661 aagcggccaa tccagtcatt gatacgttgg aattagctag atatctgtat cctcaattca
     6721 aacgtttcgg attaggtgtg ttatctaaaa agtttggtgt tagcttggaa cagcatcacc
     6781 gagcaatcta tgatgctgaa gcaactggtc acttagcatg gatctttgtg aaagaggcaa
     6841 tggacaatca taacatgctt tatcatgatc agctaaatga gcatatcgga gaaggagatt
     6901 cttacaaaag ggctcgacct ttccatgtaa cgattttagc aaaaaatcag gcaggactga
     6961 aagatttgtt caaactgatt tcaatgtcga atgtcgaata cttcgaacgc gtcccacgaa
     7021 taccacgctc ccaattgaaa aagatgcgtg aaaatctatt gatcggctca gcttgtgata
     7081 agggagaaat attcgaagcc atgatgcaaa aaggggtaga agaggcaaga aaccgagcaa
     7141 aattctatga ttatattgaa gttatgccaa aagcggtcta tgcgcctttg atcgagcaag
     7201 aattggtaaa aaatgaacat gatttggaag aaattatcca aaatttggta gaaatcggaa
     7261 aaagtttgga taaaatcgtt gttgcaacag gtaatgtaca ttatctgaac gaagaagatg
     7321 ccatttaccg aaaaattttg atcaattcta tgggcggagc gaatccgttg aatcgtcata
     7381 gtttgccgga tgtccatttc cgaacaaccg atgagatgct aactgcattt cactttttag
     7441 gggaagaaac agcgaaagag atcgtagtag aaaatacgaa taaaatcgca gatatctgcg
     7501 aagaagtgat tccagtcaaa gatgaacttt atacaccgaa gattccaggt tcagaagatg
     7561 aaatatcaga attgagttac acaaaagcaa aacaaatgta cggagatccg ttacctgaga
     7621 tcattcaaaa acgattagaa aaagagttga attctattaa tggtaatggc ttctcggtta
     7681 tttatctgat cgcccaaaaa ctagtgcata aaagtaatga agatggctat ctagtaggtt
     7741 cacgtggctc tgttggctct agctttgttg cgacgatgac aggaatcact gaagttaatc
     7801 cattagcccc tcattattat tgtccagagt gccaatattc cgagtttttt gaagatggta
     7861 cgtatggttc tggatttgac atgccagaaa aacaatgtcc aaaatgtggt gcacgcttaa
     7921 acaaagacgg tcatgatatt ccatttgaga catttttagg attccatgga gacaaagtgc
     7981 ccgatattga tttgaacttc tcaggtgatt atcaagcaga agcccataac tatacgaaag
     8041 tattgtttgg cgaagactat gtttaccgtg ccggaacgat tggtacagtc gccgacaaaa
     8101 cagcatatgg ctatgtaaaa ggatacgaaa gggacaataa tctgcagttt cgaagtgcag
     8161 aagtcgacag attagcaaaa ggcgctactg gagtcaaacg gacaaccggc cagcatccag
     8221 gggggatcat cgttattccc gattatatgg atgtctatga ttttacgccg atccaatatc
     8281 cagcagacga tcaaaattca gaatggaaaa cgactcattt tgatttccac tcgattcacg
     8341 ataatgtatt gaaattagat attctagggc atgatgaccc aactgttatt cggatgcttc
     8401 aagatttatc agggatcgac ccacaaacga tccctacgga tgacccggaa gtgatgcgga
     8461 tatttgccgg tcctgaagta cttggagtaa gtcaggaaca aatttattcc aaaacaggga
     8521 cattaggtat tccagaattc ggcactcgtt ttgttcgagg gatgttagaa gaaacgcatc
     8581 ctacaacttt tgcagaacta ctgcaaattt ccggactatc tcatggtaca gacgtatggt
     8641 taggaaatgc ggaagaattg attcgacgag gagatgcgac actggcggaa gtaatcggct
     8701 gtcgtgatga tattatggtt tatttgatcc atgctggatt agatagcggg atggcattta
     8761 aaatcatgga aactgttcgt aaaggacaat ggaacaagat ccctgatgaa ttgcgagaaa
     8821 cctatctatc ggcgatgaaa gaaaataatg tgcccgactg gtatatcgat tcttgttcaa
     8881 aaatcaaata tatgtttccg aaagcccatg ctgcagctta cgtattgatg gctcttcgtg
     8941 tcgcttattt caaggtttat ttccctattt tgtattactg tgcatacttt tctgtacgtg
     9001 cagacgactt tgatctagtc tccatgtgca aaggaaaaga tgctgtgaaa caagcaatga
     9061 aagaaatcac ggacaaggga ttagatgctt ccgtaaaaga aaagaatcaa ctgacagtgc
     9121 tggagttagc caatgaaatg ctggaacgag gattcaaatt tggaatgatt gatttgtata
     9181 aatcggatgc cgttaacttt gttattgaag gtgatacgtt gatcgcacca tttagagctg
     9241 tcccaagttt gggaaccaac gtggcgaaac aaatcgttga agcaagaaaa gacggtccat
     9301 ttttatccaa agaagatctt gccacccgcg gtaaagtttc aaaaacattg atcgaatata
     9361 tgaacgacaa tggcgtattg aaagatctac cagatgaaaa tcaattatcg ttattcgata
     9421 tgctataagc atctgttttc ctgtctgctt gtaattttaa gcagagtatg gtatagttct
     9481 tataggtaat gatactaact gtgagtgagc ggatttttcg ctcactcttt ttaatggaat
     9541 tacgcactta atttttaagg aggcgaaact tttgagtagc gtcgttgaaa cagtcacaga
     9601 aatggtgaca ccgattttag atgaacaaaa ttttgaactt gtcgaagtcg aatttgtaaa
     9661 agaaggaaaa agctggttct tacgagtatt tattgacaaa gaaggcggta tcgatatcga
     9721 agaatgcgcc tttgtcagtg agaaactgag tgaaaaactt gatgcagcag atccagatcc
     9781 gattcctcaa gcttatttcc tggaggtatc ctccccggga gcagagcggc cattgaaaaa
     9841 agaaagcgac tatgaaaagg cacttggtga ttatgtccat gtctccttat accaaccagt
     9901 agatggcgaa aaacaatatg aaggatttct tcaatcattt gatgctgaac agcttacatt
     9961 gaaaatacgc ataaaaacac gagaaaaaga attgactttt gaccgaaaaa atattgcaaa
    10021 agctcgctta gctatccaat tttaatcacg gagaggaatc agaaaaaaat gagtaaagaa
    10081 atgttaaatg cgttagacgc tttagaagct gaaaaaggga tctcaaaaga aatcgtgatc
    10141 gatgctttag aagcagcatt ggtttctgca tacaaacgtc actatggcca agcacaaaac
    10201 gtcgaagtgg aatttgatca gaaaaaagga aagatccatg tctatgcggt aaaagaagta
    10261 acggaagaag tgatggattc acagttggaa gtctcattga aagatgcact tttaatcaat
    10321 ccagcatatg agatcggtga tacgatccgt tttgaagtaa caccaaaaga ttttggacgg
    10381 atcgctgcgc aaacggctaa acaagttatt ttacagcgtg tacgtgaagc agaaagaacg
    10441 attatttata acgaattcag tgcttatgaa aaagacatca tgcaaggaat cgtcgaacgc
    10501 caggataaac gttatattta tgtcaatctt ggtaaaattg aggcagttct ttctaaacaa
    10561 gatcaaatgc caaatgaatt ttatcaacca catgatcgaa tcaaagttta tgtttcccga
    10621 gtagaaaata cgtctaaagg tccgcaagta tttgttagcc gcagccatcc agacctattg
    10681 cgtcgcttat ttgaacaaga agtaccagaa gtctatgatg gaatcgttga aattgtcagc
    10741 atcgcccgtg aagctggcga tcgttcaaaa gtggcagttc gctcaaatga tccaaatatc
    10801 gatgcagtcg gtacatgtgt cgggccaaag ggacaacgag ttcaagcaat cgtcaatgaa
    10861 ttaaaaggcg aaaacatgga tatcgtcgaa tggaatgaag atccagctgt attcatcgca
    10921 aatgcattga atcctgcaca agtcatggat gtgatcttcg atgagacaaa tccaaaggcc
    10981 tgcacagttg tcgttccaga ctaccaatta tcgctagcaa tcggtaaacg cggacaaaat
    11041 gcacgtttag ctgctaaatt gactaaccat aaaatcgata tcaaatcaga atctgacatg
    11101 acggaatttt atgaaaatca agaacaacaa aaagaaacag aagaactgca tgatgaagca
    11161 atcgttcagt cagatttaac agacgatgaa tatgaaacaa ttgcattcaa caatgaaacg
    11221 gtcgaagaaa aaccagaagc ttaactttag taaggaggca aagagatgaa acaaaggaaa
    11281 attcctttac gcaaatccgt ggtttcaggt gaaatgaaac ctaaaaaaga gatggtccga
    11341 atcacccgtt caaaagaagg ggaagtatcg atcgatccaa ctggaaaaat gcctggacga
    11401 ggagcgtatg tctcacttga gccggaagaa gtacaacaag cttgggataa aaaaattctc
    11461 gatcgagtac tcgaaacaac attaacagat gagttttatc aagaacttct tgactatgtc
    11521 actcaccaaa aagcacgtaa agagttattt ggaaaatgaa tacagttaat cggcaaaaag
    11581 ccatgaatct catcggtcta gcgatgcgcg cgggaaaact aatcacgggt gaagaactga
    11641 ccattgctga aatccgcaaa caaaaagcaa aattggtgtt tgtggcaacc gatgcaagtg
    11701 agaatacaag aaaaaagata aaagataaga gttcatatta tgaagttcct tgctttgagc
    11761 tattttctga agcagaaatc acgcagatga tcggaaaacc tcgcaaggta ttcggaataa
    11821 tggatgccgg atttgcgaag aagaccaagg agctaattga aggttaggaa ggtgattgca
    11881 tgggcaacaa aagaatctat gaattagcaa aagaactcaa aaaatctagt aaagacgtcg
    11941 tggaaaaagc acaacaactt ggcatcgatg tgaaaaacca tatgggtgcg attactagca
    12001 gtgatgagaa gaaattgcaa caagcgttca aaaacccaag cacgaaacaa caagctgcac
    12061 aacaagcaag cagaaaacca gccaatcaac agccgaacga tcaacgtaaa aaagaaacaa
    12121 cgaacaatca acaaaaaaat aaaaataacc gcagttatca agatcgcggt caagggagcg
    12181 gtcaagtgaa tcaaggaaaa aaaccaacaa caaaccaaaa taatacggga aataccagca
    12241 gccaaaatcg tcaaggaacg agccaaggcg gcaatcaaaa ccgtactaac aataacaaca
    12301 aaccaaacaa taacaatcgc ggcaaattta ataataataa ccgtaatcgt tataataaaa
    12361 aaggtaaaaa aggcaaacag caacaatcaa ctaaaccagc tgtaccacca cgcaaattcc
    12421 gtgaattgcc agatgtatta gagtatactg aaggtatgaa cgtagcagat attgcgaaaa
    12481 aaattcatcg tgagccagca gaaatcatta aaaaattatt tatgcttggt gtcatggtca
    12541 atcaaaacca agcactggat aaagatacga ttgaactgtt agcgacggac tatggcatgg
    12601 aaccacaaga aaaagtccaa gttgacattg ctgatatcga taaattcttc gaggcagaag
    12661 aagtcgatcc agataaatta gtctcacgtc cgccagtagt aacgattatg ggacacgtag
    12721 accacgggaa aacaacctta ttggatacat tgcgccattc acgtgtaaca agcggcgaag
    12781 ccggcggaat cacacagcat atcggtgcct accagatcga tattgatggt aaaccaatca
    12841 ctttcttgga tacaccagga catgcggcct ttacaagtat gcgtgcacgt ggagcaagta
    12901 tcacagatat cacgatctta gttgttgctg cagatgatgg cgtcatgcca caaacagttg
    12961 aagcaatcaa ccatgccaaa gctgcaggcg ttccaatcat cgtcgctgta aataaaattg
    13021 ataaaccagg tgcaaatcct cagcatgtta tgcaagaatt aagtgagtat gaattaattc
    13081 ctgaagcatg gggtggagaa acgatttttg ttgagatctc agcgaaattc ggccaaaaca
    13141 tcgaagaatt gctggaaatg atcctgttag tcgcagaagt agaagattta aaagcagatc
    13201 caacacaacg cgcaatcgga acagtgatcg aagcacgtct tgataaaggt aaaggtcctg
    13261 tgtcaacctt acttgtccaa gaaggaacac ttcacgttgg tgacccaatc gttgtcggta
    13321 atacttacgg acgtgtccgt gtaatgacaa acgatcttgg ccgtcgcgat aaagaagcag
    13381 gacctgcaac gccagtagaa atcactggct tgaatgatgt acctcaagca ggtgaccgtt
    13441 tcgtagtctt cgaagacgaa aagacagcac gtgcagcggg tgaagaacgc gcaaaacgtg
    13501 cattattgga aaaccgtgct gccacaagcc gtgtgacttt ggacaactta tttgaaagcc
    13561 tgaaagaagg cgaattgaaa gaagttaacg taatcatcaa agcagatgta caaggttcag
    13621 cagaagcact ggctgcatca ttgaagaaga ttgatgtgga aggtgtccgt gtaaaaatcg
    13681 tccattcagc tgtcggagca atcaatgaaa gtgatgtaac cttagcagca gccagcaacg
    13741 ctattatcat cggtttcaac gttcgtccta caccgcaggc aaaagtgcaa gctgatactg
    13801 aagaagtcga tattcgtctt caccgtatca tctataaagc catcgaagaa atcgaaactg
    13861 cgatgaaagg gatgctagac cctgaatttg aagaaaaaat caccggtcaa atgatcgttc
    13921 gtgaaacctt caaagtttct aaagttggta cgattgcagg tgcctatgta acagaaggat
    13981 atattcgtag agatagcggt gtccgtgtaa tccgtgacgg aatcgtaatc tatgaaggcc
    14041 aacttgcaag tctgaaacga ttcaaagatg acgtgaaaga agtcaaaatg ggttatgaat
    14101 gtggtgcgat gattgaaaaa ttcaatgaca tcaaagtaga tgacgtaatt gaaggattta
    14161 tcatggaaga agtcaaaaat gactaaaaac tgataaaagg aggccaatca ttatggctaa
    14221 ctaccgtgat cgccgagtcg gtcaagaaat cttaaaagaa gtcaatgata ttttgcgcaa
    14281 aaaggtccgt gaccctcgtg tggagaacgt aacgatcaca gatgtgaaag ttacggggga
    14341 cttacagcaa gcaacgatct attacagcat tttatctgac ttagcatcag ataagaaaaa
    14401 agcacaagat ggattgaata aagcatcagg attgattcgt cgagagttag ggcacaatct
    14461 aagtatttat aagacacctg aattgttctt tgaacttgat gaatcagttg tctatgggaa
    14521 ccatatcgat gaattgcttc gcgacttgaa caaagactaa tcaaaaaatc ccgcaaatcc
    14581 tgtgaaatca ggggttgcgg gatttttttg ttttcattaa gacgtattac ctaagagttc
    14641 catgacttta gtctggtagc gtgtcagcta accaaaaaag agaaaaagaa aagtaaaaaa
    14701 ttcgactttc ttttttctat agtatataaa tttttgcata tagataaaga tcaagatacc
    14761 gataacggaa gctagattgt aatctatttt agtgacagtc aggtatagcc aagtaactag
    14821 aagatcagtg gaacctgcac cataaaagtt tgagtttgtc ggtccgttga cagttaaaag
    14881 aaaaatcacg ttgaagttat tgatatttcc gattacgccg cttcgatttg gtcacgatcc
    14941 aaattttgga taatccctgt agaaattagc atagtagctg ggataccaat tcacatattg
    15001 acaaagataa cagagacttt tacataaaac ggatcaatca ggaaaggaat tgctcgatcc
    15061 agccccagtt ttccaacaat gtgttaactg gcctagcgtc attcagcaaa ttatgcatga
    15121 tgaggagcga aatgaactgc ggaacagcca ttgtgataac gacaagtttt ttggatcaat
    15181 gtaaaaaacg tcagcgaaag gaaaaatttc gtggtttttt atacctgttc atttggctag
    15241 atataaaatc caaacaaatg aacagaagtg cttattaggt ttcaattggg agtatctgcg
    15301 ctagaataag agtaataatg aaaaggagtg ttatgtatgg attattctag taaagaatac
    15361 tttgataaaa tgacggcttg gtggcgtgct gctaattatc tttctgttgg gcaactttat
    15421 cttaaagata accccttact gagacgtaca ctgaaaccag aagatgtcaa aaaacatcca
    15481 atcggtcatt ggggaacgat tcctggccaa aattttattt atgtccattt gaaccgagtc
    15541 atcaataaat atgatttgaa catgttttat atcgaagggc caggacacgg cggacaagtc
    15601 atggtatcga atgcttattt agatggaagt tacacagaga tctatccaga agtaacggaa
    15661 gatgagacag gaatgcaaaa attattcaag cgattttcat ttcctggagg cattgcttct
    15721 catgcagcac cagaaacacc aggttctatc catgaaggcg gagagcttgg atactctctt
    15781 tcacatgcag tcggagctgt attagacaat cctgaagtaa tctctgctgt cgtgattggt
    15841 gatggtgaag cagaaaccgg tcctttagct ggaagctggt tctcaaatgt ctttatcaat
    15901 ccagtgatag atggagctgt tttaccgatc cttcatttaa atggtgcaaa aatagccaac
    15961 ccaacgatct tggcacgtaa atcagatgga gaattagcaa attactttaa cggacttggc
    16021 tgggagcctt tctttattga aggaaatgat ccagaaaaat taaatcctgt tatggctgaa
    16081 aaaatggatc aagcaatcga gaaaatcaaa tccatccaaa aagaagctcg cctgaagaca
    16141 gcaacagatg tcgtgatgcc aaaatggcca gtattgatcg tacgcacacc aaaaggatgg
    16201 accggtccaa aagaatggga cggggaacca attgaaggaa ccttccgagc acaccaagta
    16261 ccgattcctg tagatcaaga acatatggat catgcagatg ctttactccg ttggctgaaa
    16321 tcctatgaac cagaaaaatt atttgatgca caaggacgta ttttagaaga aatcagagaa
    16381 attgcgccta ctggtgacca aagaatggca aaaaatccta tcaccaatgg tggaatcgat
    16441 ccaagaccgc ttattatgcc agattggaaa aaatatactt tgcaatttga aaaaccaggc
    16501 tccgttaaag cagaagatat gaccgaactt ggtaaattcg ttcgcgagat cattgagaaa
    16561 aatccagaaa atttccgtat atttggacca gatgaaacaa aatcaaaccg attgaatcaa
    16621 gttttcaaaa cgacaaatcg ccaatggatg gaaaaaatcg aaccagaaaa tgatgaatgg
    16681 ctttcgccat ctggtcgcgt catcgacagt caattatcgg aacaccaaga tgaagggttc
    16741 ttagaagggt acgttctgac tggacggcat ggtttttttg caagttacga atcattcttg
    16801 cgagtggttg actctatgtt gactcagcac ttcaaatgga tacggaaatc acatgacctt
    16861 tcttggcgaa aagattatcc ttcgctgaat ctcattgcaa gctcaacagt cttccagcaa
    16921 gatcacaatg gatatagcca ccaagatcca ggaatattga cacatttggc agaaaagaag
    16981 gcggagttca tccgtgaata tctgcctgcg gatgcaaata cattgctcgc agtcatggat
    17041 aaagcattcc gctcgtcaga aaaaatcaat ttgatcatct cttccaaaca tccgcgtgct
    17101 caattttact cagcagaaga agcagccgtc ttagtgaatg aaggcttgaa aatcattgat
    17161 tgggcttcga cagcaaaaga ggaggaacct gaattggtca ttgctgctgc aggaacggaa
    17221 tctaacttag aagcattagc agctgtaact ttgctgctgg aagaattccc aaaactaaaa
    17281 attcggttca tcaatgtcgt agatttattg aaattacgtc atccaagtca agatccgcgt
    17341 gggttgagcg atgaagaatt tgatcaatat tttacaaaag acaaaccgat cttatttgct
    17401 ttccacggtt atgaaacatt ggtccgcacg attttctttg atcgtcataa ccatcattta
    17461 atgattcatg gctacaaaga aaatggagat atcacgacac catttgacat gcgtgtagta
    17521 aatgaacttg atcgttatca tttggcaaaa gacgcagctt taaaaatcaa aggaagtcaa
    17581 gcagaagatt ttgcggaaaa aatggatcag aaattacaag aacatcaaaa ctatattcgt
    17641 gaaaatggta ttgatctgcc agaagtattg gattggaaat ggaaaaatct tgaccaataa
    17701 aatgataaaa tcgatcccgc aaaacagaat tttttacatt gtattatttg tcaaattaag
//
LOCUS       DACCXO010000050        17757 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.051, whole genome shotgun sequence.
ACCESSION   DACCXO010000050 DACCXO010000000
VERSION     DACCXO010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.051"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(11..139)
                     /locus_tag="IVY70_001573"
     CDS             complement(11..139)
                     /locus_tag="IVY70_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP7958706.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(142..1515)
                     /gene="rlmD"
                     /locus_tag="IVY70_001574"
     CDS             complement(142..1515)
                     /gene="rlmD"
                     /locus_tag="IVY70_001574"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP7958707.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1626..2681)
                     /locus_tag="IVY70_001575"
     CDS             complement(1626..2681)
                     /locus_tag="IVY70_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP7958708.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2691..4121)
                     /gene="gatB"
                     /locus_tag="IVY70_001576"
     CDS             complement(2691..4121)
                     /gene="gatB"
                     /locus_tag="IVY70_001576"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP7958709.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4118..5590)
                     /gene="gatA"
                     /locus_tag="IVY70_001577"
     CDS             complement(4118..5590)
                     /gene="gatA"
                     /locus_tag="IVY70_001577"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP7958710.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5590..5895)
                     /gene="gatC"
                     /locus_tag="IVY70_001578"
     CDS             complement(5590..5895)
                     /gene="gatC"
                     /locus_tag="IVY70_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP7958711.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6001..8037)
                     /gene="ligA"
                     /locus_tag="IVY70_001579"
     CDS             complement(6001..8037)
                     /gene="ligA"
                     /locus_tag="IVY70_001579"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP7958712.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKDTIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8054..10288)
                     /gene="pcrA"
                     /locus_tag="IVY70_001580"
     CDS             complement(8054..10288)
                     /gene="pcrA"
                     /locus_tag="IVY70_001580"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP7958713.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10443..12002
                     /locus_tag="IVY70_001581"
     CDS             10443..12002
                     /locus_tag="IVY70_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP7958714.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12113..14044)
                     /locus_tag="IVY70_001582"
     CDS             complement(12113..14044)
                     /locus_tag="IVY70_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP7958715.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14062..14976)
                     /gene="pfkB"
                     /locus_tag="IVY70_001583"
     CDS             complement(14062..14976)
                     /gene="pfkB"
                     /locus_tag="IVY70_001583"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP7958716.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(14973..15725)
                     /locus_tag="IVY70_001584"
     CDS             complement(14973..15725)
                     /locus_tag="IVY70_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7958717.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15884..16093)
                     /locus_tag="IVY70_001585"
     CDS             complement(15884..16093)
                     /locus_tag="IVY70_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958718.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16104..17432)
                     /locus_tag="IVY70_001586"
     CDS             complement(16104..17432)
                     /locus_tag="IVY70_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958719.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT         4701 a         3825 c         3213 g         6018 t
ORIGIN      
        1 tttagcacgt ctatttatta aagattttag ggccttttct catgttttta cgatcattct
       61 taggtgtttc catttgtttg gatcggcctc caccttgttt ctttttgatc aattctttca
      121 ttttttcttt ttggtccatt tttattctcc tttaacgaat gaagcgacta attcgatatg
      181 atgggtttgc gggaaaagat ctacaggctg aactttcttc acggcataac ctgcttctgc
      241 aaataactta gcatctcttg caaaagttgc cggattacat gagatataga cgattcgttc
      301 tggtgacatt tcgattgctg attcaataaa atgatgatct aatccttttc gtggagggtc
      361 aactacgatc acagatggtt tgatcccttt ttcttgccat tttttcataa ctttttctgc
      421 ttttccgact tcataatgaa cattttcgat tttattattt aaagcattga actgtgcatc
      481 ttcaattgct tctggaataa cttccatccc atacacctta tccactctgt ctgctaaaga
      541 caagccaatc gtacctattc ctgaataagc gtcgatcaca atgtcttttt tatctaactc
      601 ggcaaactcg atggctgttt ggtacagata ctctgcttgt tgagtattta cttgataaaa
      661 tgactttgct gagattcgat aggtttttcc taatagctga tcttcgatat agtctctgcc
      721 atataaaact ttttctttgt ctcctaaaat cacatttgtt ttttcttcat tgatattttg
      781 gatgaccgaa accacttctg gcaattcttc atgaatagct tcagcaattt ccttggattt
      841 aaagaatttt tctttacgag taactaaaac gaccatcatt tcatgggaat aatgaccacg
      901 acggataatg atgtgacgaa gctgtccttc atttttttct tcattatatc ctcgtacttg
      961 gaaccgttgc aagatatctc tgactacttt gattgcttga tcgatgacag gatcttggat
     1021 ataaaaatct tcgataggga ctagatcatg gctattcttt ttgtaaaaac cggtggctaa
     1081 ctgtccatcg atacggcgaa cagggatctg tgctttatta cggtagccga atggttctgc
     1141 cattccgacc gttggaagta caggaacttc tggcatctta gcaatcttcg ccattacatg
     1201 ctctacttgt ttttgtttga agacaagctg ttgttcatac ttcaaatggg ctaaaggtgc
     1261 gatacctatg cgaagtaatg cattgttttc tacaggctga cgatcaggtg attgtgtaat
     1321 aaatgtttcg acttttccat atccaaactt attgcctgct tttactacta gtatttctac
     1381 ttcttctcca gggagtgcat tttcgataaa taatggataa ccatcgattt ttgctacccc
     1441 cattccttca taactcaagt cgacgatttc gactgtatat cgttcgtttt tctttactat
     1501 ttgctgttct gtcatatttt gctcctttaa gcctattttt caatcctact gaactctact
     1561 ataaatcagc taattacaca actaaaaccg gcactgacct tactacgttc agcaccggct
     1621 tttgattaat tcttttgatt aggtgtttcg tctgctattt ttccgtcacc gtcgatatct
     1681 tcatccgtta aacgttcgac ttcttttacg aattctttgc tgacaacctc taattcttct
     1741 tcatctgtac ctgtgattgc ttcatcagga atctgatcgg tattggcaaa gaactcaatg
     1801 tgttgatgaa gattttggaa atgcatcggt gcttccccgc cgtattcacc atctaagttg
     1861 atcattactg gtcgattgat atcatcttct gtttcaacga acaaactgct tgttttggta
     1921 tagattattc ttggatcatc gacatgtcga ccgccattca gcatgagtgc tgcaatatgc
     1981 aagatctcaa agatatttgc tgtcttcaca atgattaaag aaaacttccc atcatccaat
     2041 ttggcatctg gcgcgatctg ttcgaatcct ccaacagagt tcgtcagccc taggaaaaac
     2101 atcgaagcat ttccgatata ttccccttca tcatacttca agcgcatttt gattggcttt
     2161 actcgaggga gcatctctgc tcctttcgca agataagcaa gataaccaaa aatactcttt
     2221 agttctgatg gaacttcata agtcaattct gtcaaatagc cacctgcagc gatattgatg
     2281 aagtaactgt tatcagaaag gccgatgtcc atttttacgg tttggtgttt ttttatcact
     2341 tctgctgctg ctttaatatt atctctagga atttttaatg ctcgagcata gtcgttcgtt
     2401 gtaccagctg ggatgatggc cattttagga cggtgttcaa gcggagcgat tccattaacc
     2461 acttcgttga ttgtgccatc gccaccagct gccactacta gatcaaaacc tagttctgcg
     2521 actctacatg cttcattctt ggctgaatct ggttctggtg tagttgcata ggcgctggct
     2581 tcaaatcctg cttcttctaa tgcctgtagg atatccgcca aatttttttt caataattct
     2641 tttcccgagg tggggttata aattacgcgt gcttttttca ttttcagcct ctatcgtttc
     2701 gctaattcat ctttaagtag cttattcacg acaccaggat ttgcttgtcc tttagttgct
     2761 ttcatgattt gaccgactag gaagccaacc gcacgatctt ttccattttt gaaatcatcg
     2821 acagattgtt ggttgttatc caaaatttca ttaatgattg gcagtagttt gttcggatca
     2881 gataattgca cccaaccatt tttttcaact acttcttggg cttctccgcc atttgtgatc
     2941 aattcacgga agactttttt ggcgattttt gagctgatcg ttccatcggc gatcaaacga
     3001 atcattcctg ctaagttctc aggtgtcaat tttgtctcag ctaattccag tttctcactg
     3061 ttcaagtaag cagaaacttc acccatcaac cagttagatg cttgcttggc atctgcacca
     3121 ttttccaaaa cagcttcaaa aaaatcagac atttcttttg taagtgtcaa taccattgcg
     3181 tcatactctg gaagtccaaa ctcacggata tagcgttcgc gacgagaagc tggcatctca
     3241 ggcaaactag cttttacttt ttgaatccat tcgtcatcga tcacgaattt cgggatatca
     3301 ggctctggga agtaacgata atcactagac ccttctttga cacgcatcaa aactgtttta
     3361 ttcgttgttt cgtcaaaacg gcgtgtttct tgttggattt ctcctcctga aagaagaact
     3421 tttgcttgac gtttttcttc aaaagctaaa ccttttttca cgaaagataa agagttcaag
     3481 tttttcaatt ctgctttcgt accgaactct tcttgtccat aagggcgaag agaaatattg
     3541 gcatcacagc gcatagagcc ttcttccatt ttcacatcgc tgacttctgt aaattggata
     3601 attgatcgca atgcttctag ataagcgtat gcttcttctg gtgaacgcat atcagcttca
     3661 gaaacgattt cgatcaaagg cgtgccttgt cggttcaaat caacgtaaga ataaccgcca
     3721 ataccatgca tgttttttcc agcatcttct tctaaatgga cacgttcgat ccggattttt
     3781 ttctttttac cttctacttc gatttcgatc catccgtcat gaccgattgg ttgatcaaat
     3841 tgggaaattt gatatgcttt tggattatct gggtaaaagt aatttttccg gtcaaaatga
     3901 gtatctttag agatttcaca gtttaatgcc aaagctgctt tcatcccgaa ttccagcgca
     3961 gctttattca ttactggcag cacgcctgga taaccccagt cgatcacgtt tgtattgctg
     4021 tttggttctg caccaaaatg agctggcgct ggagagaaaa tttttgagtt ggtttttaac
     4081 tcgacgtgga cttctagtcc aatgactgtt tcaaaattca ttcgtctttc cctcctaaaa
     4141 tagctggttg ttgtttatgg aattctgttg cttgttcaaa cgcatatgcc gcttgataca
     4201 tcgttttttc atcaaaataa ttaccgatga tctgcaatcc aactggtaat ccttctgata
     4261 aacctgccgg tacagacata cctggaagtc cagccaagtt gacagggatc gttaagatgt
     4321 cactcatgta cattgtgatt ggatcgttaa tgttctcacc gattccgaat gcaactgttg
     4381 gtgaagatgg cccgatgatc aagtcataat ctgcaaagac tttttcaaaa tctcgtttga
     4441 tcaatgtacg gacttgtccc gcttttttga agtaagcgtc ataataacca gcacttaagg
     4501 agaatgtacc taacatgata cggcgtttta cttcttcacc gaatccttct gaacgtgagt
     4561 tgacatatac atcatctaat gatttcacat tttctgagcg ataaccgtag cggataccat
     4621 cgaatcgttg caagttggaa cttgcttctg atgaggcaat gatataataa acagccacac
     4681 cgtattttga atgaggcaag ctgacttctt ccacaacagc acctaatgaa cggaatgttt
     4741 ctgctgcttt caatacagct tcttttacgc caggggccac accttcgcct aagtattctt
     4801 taggtaaagc aatcttcatt cctttgatgt cgcctgtcag tccctctgca aaatcaggaa
     4861 ctgaaacgcc tgaagaagta ccgtcttttt cgtcataacc gctgatagct gtcaatgcta
     4921 atgcattatc tttcaccgta cgggtcattg gtccgatttg gtcaagagat gaagcaaagg
     4981 caatcaaacc aaaacgtgag acacggccat aagtaggttt catccctacg atgccgttaa
     5041 aagctgctgg ttggcggata cttcccccag tatcactacc taaagaaact ggaatttgtc
     5101 cagctgcaac tgctgctgca gaaccaccag aagagccacc aggtactttt gttgggtccc
     5161 aagcattttt tgttttcttg aaataagaag tttctgtact tccacccata gcaaattcat
     5221 ccatgtttag cttaccaaca gggatcatat ctgcttgata gattttatcc atgaccgttg
     5281 catcataaat tggttcaaaa ttgtaaagga tcttagatgc agcggttgtc aagatatctt
     5341 ttgttacgat attatccttg ataccaattg gaataccagc aaggacgttg ctttcatcaa
     5401 tccctttttc atctaaagct tttgcctgtt ccaatgcttt ttcttcgttc aatgtaatga
     5461 atgcttcgac ctcaggctct gtttctttga ttcgttcaaa tgttgctttt gtcaaatctt
     5521 gtacagtgat ttctttcgat acaagcatat gatgcagttc ttctagagaa cgattgtgta
     5581 attctgtcat tatgcgccag cctccccatt atcgatgatt gcgggtactt tgataaagcc
     5641 gtcttctgtt tccggcacat ttttcaataa ttcatcgcgg ttccatccag cttgcgcccg
     5701 atcttgtcgc atcacgttta ctgtttcaac cacattcgac gtaaatggaa caccttctgt
     5761 atctacttct tccagcattt caaccatatc gatgatttta cctaattggt cggtaaaact
     5821 tgcaagttct tcatctgcga acgcaagttt tgctaatttg gcaacgtgtt tcacttgttc
     5881 ttcactaatc gccatactac cccttctttc taaacgagtt aaaaactttg ctctctattt
     5941 taacacaaga gtgccgtgtc tgaaattgtt tattgaaatt tgtctgaaaa aactcatttt
     6001 tcattcttct actgagcgac ttttagccaa tgcatctgcc atttgatctt ctgtccaaac
     6061 ttcgattcct aattcttgtg ctttggtcag cttgctgcca gcatcttctc ctgctaccac
     6121 gatatccgtt tttttcgata cgctaccagt cactttcccg ccaagatttt cgatcgtatc
     6181 ttttgcttct tcacgagtaa atcgtgttag tttaccagta aggactactg ttttttcttt
     6241 aaaaggagac tcgacttctt gcaattgggc atttcgaagc cctttgtatt cgaaattgac
     6301 gcctgcttgt tttaattcat tcatcaattc atggacttct tcgttactga aataagtgac
     6361 tacactgtcg gcaatgatat caccgatcgt atcaagcgca ataatcgact catagtcact
     6421 tttactcaat gtttctagat caccgaaatg ttctgctaaa atttttgctg ctttcgctcc
     6481 gacatgtcgg attcctaacc cgaaaatcaa ccgctcgacc gaattatctt tgctgttgtc
     6541 gattgctgtc aaaatattat ttgctgattt ttcttttatt ttgtccaacg tcaataactc
     6601 ttcttctgtc aatttgtaaa gatcagcaac atctgctact agttttttat catacatctg
     6661 ttctagtacg cgcggcccca acccatcgat attcatcgca tttcttgaaa caaagtggtt
     6721 caaaccttct ttcatttgag cagggcattt aggattgatg caccgtaagg ctacttcttc
     6781 atccaaatgg acaagttcac ttccacaaac tggacaatgt gtaggcaatt ggtattcttc
     6841 gctgtcttta ggtcgtttat ctaacacgac ttgcgaaact tctgggatga tgtctcccgc
     6901 tttataaatc agaactgtat ctttcaagcg gatatccttc aacttaatat aatcaccgtt
     6961 atgcaggctg gcacggctta ctgtcgttcc tgctactcgg actggtgtca tgatagctgt
     7021 tggcgttact acccctgttc gaccgactgt ccattcgatg ttttcgatta gcgtttcaac
     7081 ttcttctggc ggaaacttgt aagctgctgc ccatctcggt gctttgacag taaaaccaag
     7141 ttgatcttgt agcgagaatt cattcacttt gatgacgatt ccatcgattt catagggcaa
     7201 atccacacgt ttgtcatgat attcttcaat gtatgaccac acttcatcaa tcgaatgaca
     7261 tagacgtttt tcccgattcg tgtggaatcc aattttttct agctcttcta gcgcatcata
     7321 ctgggttttt gcttgcatag gaccgaaatc agctactgtg tataaaaaag tgtccagatt
     7381 tcttttcgct gtgattttag aatctaactg acgcaaagaa ccagctgcgg cgttccgagg
     7441 attcgcaaag atatcttttc cctctgcttc tctgtcttgg ttcaattgga caaatgaccg
     7501 ttttggcatg aaacattcgc ctcttacttc gatgttcatt ggttctttta atttgattgg
     7561 gaccgaccgg acagttttta ggttttcagt gatgttttct cctacagttc catccccgcg
     7621 agtcgcacct ctcacgaaat ttccatcttc ataacgcaaa gaaacggaaa gtccatctat
     7681 ttttagttca cagcaatagt caactacacg tcccactgct ttttgtacac gttgatcaaa
     7741 agctatcagc tcttctttgc tgaaaacgtc gttcaaactg taaagcggaa tatcatgagt
     7801 gactttttca aaaccttcta atacttttcc gcctactcgt tgagtgggag aatcagacgt
     7861 gatcaaatca ggatattgtt cctcaattgc tactaattct gcgtattctt tatcataaac
     7921 atagtcttcc actgttggtt tatcttctac gtagtattcg cgagaccatt ggttcagtct
     7981 tgtacgaagt tcttctgctc gtttagcagc ttcgtcaatc gtcatttcat gttccatcat
     8041 ttaccctcca ctactaagct ttttcaattg gcgcaaaggc agccaaaaga cgttttactc
     8101 cttgacttgg gaaagccaca tctagttcta gatccttcga cgtcccgctt actcggacga
     8161 ctgttcctag accccatttt ttgtgtttga ctttatctcc aggtttccag ctgttatttt
     8221 cccctccagt agcactttta gagggaacaa ccgctgtctc tggctgttta tagctatatg
     8281 ctgttttttt agcgaataca ggtgtttttt taggcgtagg acgcatgcct tcgatttcca
     8341 gtaactcctg atcgatttct tctacaaaac gagaaggtcg gttatattgc gtccgtccat
     8401 ataatgttct ggagaaagca tttgttaaat acaacgcttc ttctgctcgc gtgatcccca
     8461 catatgcgag acggcgttct tcttccagtt cgctttcttc catcaatgct cgagataaag
     8521 gaaaaacccc ctcttctaga ccaatcagga atacaactgg gaattctaat cctttggctg
     8581 catgcaaagt catcaatgtc acttgggaag catcttcttc taaattgtca atatcggaaa
     8641 caagtgccag atcatttaag aagaccgtta gtttttcttc tggtgcatca gcgtcttctt
     8701 cattttgctg ttcgaattgt ttgtcgaatt cttgtgtgac cgttaaaaat tcctccaagt
     8761 tttcgatgcg tgcttgtgct tctaatgtat tttggatttt taagtcttct agatatccgc
     8821 tacgatccaa aacctctttc gtcaattctg tcactgtcaa gtatgggatc atctgagtga
     8881 cttcttggat catctctcca aatgcaccta attgctgtcc tgcttttcca ccgatatttg
     8941 ccagatccac attttgcgct gcttcaagca gtggccattc atgaagagaa gcaaaactgc
     9001 gtagtttttc gatagatcct ggtccgatgc ctcgtttggg cgaattaacg atacgctcaa
     9061 aactgattga atcttgagga tttgccagca cattcaaata cgctaaaata tctttgattt
     9121 ctttacgatc gtagaacttg tgccctccaa ccattttata aggaatattt gctttcaaca
     9181 gagtttcttc cattacacga gattgtgcgt tcgtccggta caagatggca aagtcgccgt
     9241 aattccgatg attgcttcga atctcttctt gcatccgatc tacgataaaa cgggtttcgt
     9301 ctcgttcatt atctgcacgg tagtaagtga tcttgtttcc ttctttgttt tctgtccaca
     9361 agttttttgg ttttcgattg ctattgtttt caatgacttg gtttgctgcg ctaaggatgt
     9421 ttttcgtgga gcgatagttt tgttctagca aaattactgc agcatctgga taatcttttt
     9481 caaaatccaa gatgttctgc atatctgcac cgcgccagcc gtaaatactt tgatctgcat
     9541 ctcccacgac gcacaggttc ctaaatctac ctgccaataa attgactagc gtatactgtg
     9601 cgtgatttgt atcttggtac tcatctacat gaatataatg gaatttgttt tgataatatg
     9661 tgagtgaatc aggatgttct tcaaataatc gaatcgtatt catgatcagg tcgtcaaagt
     9721 ccatacattg attatattga agctcttttt ggtaagcagc ataacatttt gccgcaatct
     9781 cttcgtaata actgccttgc atttttgcaa aatcctgagg ggtctgcagg ctgttttttg
     9841 cttgagagat tgttcctaat atacttcgag gatcatattt tttcggatca atgttcagtt
     9901 cttttagtat ccgtttcatc aaggtcagct gttcagaaga atcgatgatt gtaaagttcc
     9961 ggttataccc gataaaatca acatctcttc ttaaaatacg cacacacatc gagtgaaaag
    10021 tcgataccca gacatcttcg cctccagatg cgagaatcgc attgacacgt tctttcattt
    10081 cttttgcagc cttattggta aaagtaatcg ctaggatgtt ccaaggattg acttcttttt
    10141 cctcaatcaa ataggcaatg cggtgtgtca gcactctcgt ctttccactt cctgcacctg
    10201 ccattagcag taatggtccg tcagtgtgaa gaacagcttc tttctgacgg gggttcatcc
    10261 cgtttaataa ttcagctttg gatgacatta acaacatcct ttcttttatc aatcttgtta
    10321 attatagcat tttcttggct ttcattaaac tagctagttc aaaatcattt gtttcttttt
    10381 atggattttt ccttgtcaat tcgttgattt cgcgctaaat taaaagaaag cgaggcgttt
    10441 gaatggaaaa aaaaggaaaa atcaagaaat tagataagac tcagatatat tttattctaa
    10501 aaggaatatt tgttggactg ctgacaggaa tcgtcgttag cctgtttcga ttatctatcg
    10561 aaaagctaag cgatttagtt cgaagtatct atgaaatgag tcgggaacag ccgatttact
    10621 taattggcct agctatttgc tgcatccttg cagcattttt cgtgggttat ctggtcaaaa
    10681 atgaaccgga tatcaaggga agcgggattc cacaagtaga gggacagctt cgcggggaat
    10741 tatcaatgaa ctggttctct gttttatgga aaaagttcat tggcggtgta ctttcagttg
    10801 gtgcgggcct ttttctaggt agagaaggac cttctataca gcttggcgca tccattggac
    10861 aaggagctgg acaattgttt cggagtccat cttcagaaga aaagatttta atttctagcg
    10921 gtgcaagtag cggacttgca gcagcattca atgctcctat cgcaggatta ctctttgttt
    10981 tagaagaaat ccatcactcc ttttctccac ttgtttggct gacatcattt gcttcagcaa
    11041 tcactgctaa cttcgtctct ctttactttt tcggcttgag acctgtttta tatctaggtg
    11101 accttccttc tttgccgttg aaatattatg gttccttagt gatcttagga gtacttctag
    11161 gcatcttagg ttttatttat cagaaggtct tgctggcttt gccaaaattt tacaaccgat
    11221 tgcctttacc agcttatttt cacgggttcg ttcctttctt gctgattttg ccaattggtt
    11281 atttatggcc tagtactcta ggcggaggca atcaaatcgt tttagctttt ggacatgctt
    11341 ccttgccaat cattatgatt atcggtttgt ttgttcttcg attcgttttt tccatgattt
    11401 cttacggtgg tggtcttcca ggggggattt tccttccgat attgacattg ggggctttaa
    11461 tcggtagctt ttatggaaac attatggttg atttcgggat ggatccaatc tacatcaagg
    11521 attttgtcgt tattgccatg gctggttatt tcacagcgat tggaaaagca ccgctgacag
    11581 caattatttt agtgacagaa atggttggct ctcttaatca tctgatgcct ttaggattgg
    11641 ctgctcttgt tgcttatatc gtcaatgacc ttctcggcgg taatccaatt tacgaatcat
    11701 tattggaacg tctacttgga aatactcaga ccgcctcact aaaaggtgca aggacaactt
    11761 ttgattttcc agtaacggca gagagttctt tagacggatt aatggtacga gacttcaatt
    11821 ggcctaaaaa tatgctgcta atctctatac gcagaggaaa tcaggaaatc ttgactcacg
    11881 gtgatacgat catgaatgta ggagatattt tggttatctt gactgatgaa gcaaacttgc
    11941 caagaatcaa gcaggaaatc gaggctaaat cttctatcac aaatttgaag caatcatttt
    12001 aacgaacatg gaaaaactat tttacaacaa ctattcatct acactacttg attctatatg
    12061 attctataaa aaaagaagag actgggaaaa accagcctct tcttttttat ttctatgctt
    12121 ctgctgctgc aggagctttt ctcaataaac cataaatcac accagatacg actgctccga
    12181 tagcaataaa caccaaatac atgaccgggt gactcagtaa gaatacaacg aaaatcccgc
    12241 catgaggagc cagtaatttg atcccgaaag ctccgactaa tccgccagtt aaagcagaac
    12301 caataataaa acttggaatg gcacgaatcg ggtcagctgc tgcaaatgga atcgatcctt
    12361 ccgtgacaaa cgatagtccc atcacgatgt tcgtcaaacc agcatcttga tctgttttgc
    12421 tgaatttatt cttaaacaaa cgtgtagcaa cgaaaattgc caatggaggt accattcctc
    12481 cagccataac agaagccatc acgacacttc cgccagttgc aacagtcgta gctaaagttg
    12541 ctgtaccaaa cacataagcg gctttgttga ttggaccgcc taaatctgcc gccatcattc
    12601 cagctaataa tgcaccaagt aaagcagcat tcgaaccact catacttgtc aagaaaccgt
    12661 tcaatgcatc attgattgct ttcataggga cattgaccag caacatcaag aaaccagtaa
    12721 tcagtaagcc aaacactgga taaaagagga tcgttttaat tccatccaat gattttggta
    12781 agttcttgaa taatttacgt aaaaatacga tgacataacc agctaagaat ccaccaatca
    12841 gtgcacctaa gaatcctgca ccgcctgtat tagctaatgc accagcagca aatccaacga
    12901 tcaaaccagg gcggtcaccg atacttgaag caataaatcc tgctaatacc ggcagcatga
    12961 aaccaaatgc agcttgtccg atttggttga accagctagc agcttgattg taattcccta
    13021 gatttgataa ttgatcatgt ggcacgccaa taaattggtc gatcatgaaa gaaagcgcaa
    13081 tagcgattcc tccaccaata acgaaaggta acatgtgtga tacgccgttc atcaaatctt
    13141 tgtaaattct ttgtccgata gtaccatccg catttccttc ttctgaactg ctttcttttc
    13201 catcaccatg gaatactggt gcagtaccac taagggctag attgatcagc tcttctggtt
    13261 tacggatacc atcactgaca ggacgattga tcagttcttt tccgtcaaag cgattcatct
    13321 ctactttttt gtctgctgca acgattacac cttctgcacg ggcgatgtct tcagcagtca
    13381 atcggttttt gattccttct gaaccgttcg tttcgacttt gatctccaca cccatttctt
    13441 tcgctttttt cttcagagca tcttctgcca tgtaagtgtg agcgatccct gttggacaag
    13501 ctgtaacggc aacaacgaat tttctttctg gatgagcaga agcttctgca gcttgtgttt
    13561 cttgttctgc tttttcttct gcttcttttt gctcttcagc tgtttggaac aagtcttgga
    13621 cttcttctgg tgtatttgct tcttttaatt ttcctacgaa atctgcatca atcagcaatc
    13681 gagatagtgc tgctaatgct tgcaaatgtg tgtcgtttgc gccttctgga gctgcgatca
    13741 tgaaaaacag ataagttggc tgtccgtcta aggcttcata atctactcct ttttggcttt
    13801 ttgcgaataa aactgttgct tctttaactg cactgttttt cgcgtgaggc atggcgattc
    13861 catcaccaag acctgtagaa gtttgtgctt cgcgggctag aattccttct ttatatgttt
    13921 cgatatcgga aatccggccg gcatcaaaca ttttttggac catttcatcg attgcgcctt
    13981 ttttatcagt cgcttgcaaa tccatgatca tcgcttcttt gaccattaaa tccttgatat
    14041 tcatgattat cctccttgaa actaatcaat ttttgtgatt tcaacttctg gcagcaactc
    14101 atcaatcaat tcgcgagctg ccaagtcatc agaaaaagct gttgcgcttc cgcaagcaac
    14161 tccccattta aaggcttcga ttggatcttt cgtacggctg taagccccaa taaagccggc
    14221 aatcattgaa tcccctgcac ctactgaatt tttcaatggg cgaactaata cattcgtttt
    14281 ataagttcct tgttccgtga agagcaaagc accgtctcca gccattgaga tcagcacatt
    14341 ctgtgcccct tcagctagca attttttacc atacggaatg atttcttcta cagattggaa
    14401 ggttgtattg taaagctcag ctaattcgtg attgtttggt ttgatcaaca atggtttctt
    14461 aacaagtgca ttttttaggt catcacctgt tgtatcaatc acgaaatctg cacctttttc
    14521 tcgaatgata tcaatcagtt cttgataaaa tccttttcgc aggctagccg gcgcgcttcc
    14581 tgacaatacg acgatatctc cttgtcctac tgttcctaat gcttgcttca actcatcgat
    14641 ttcttcatct gaaatagatg ggcccaaacc gttaatttca gtttcagtat ctgattttag
    14701 tttgatattg attcttgtat cttcttttac ggttgtgaac aaagactgga tttgttcttt
    14761 ttcaagccac ttcgaaataa attcgccagt gaaccctcct aaaaacccta gtgctgtaga
    14821 ttgagaatcg atccgtttca aaatacggga aacattgatc ccttttcctc caggtaactt
    14881 gaagtcttcc ttcattcggt tcaaatcgcc tagttttaac ccatctacgc gtacgatata
    14941 atcaattgat ggattaagtg ttactgtgta aatcattgta ttgcctcctt aattgttgtc
    15001 ttttcacgga attcattaac gaattctaat ggacaacgct cggttatgat cgtggctgct
    15061 tccaaaggtg caacagaggt gaaggacaac tggttgaatt tggtatgatc aatcaataca
    15121 aatgtctctt cggaattttt catggctaaa cgtttaacag ctgcctcttc tgggtccgga
    15181 gtcgtgaatc cttgttcaag atgggcaccg ttcattccca taaatgcttt attaaaatga
    15241 aaatttttta gttgttctat cgcattagat cctaaaacag cattagttgc cagctttatc
    15301 gttccaccta aaataatggt agcaagctcc aaatcaacta aatttgccgc atgttgaacg
    15361 gaatttgtca ctactgtgat ctgttttcct tgcagaaaag gaatcatttc taacgtagta
    15421 gagccagcgt ctaaataaat gacatcttcg tcattaatgg tttcagctgc cgatttggcg
    15481 atttgctgtt tttcgtgaac gtttttgagt gatttttcac tcatattctg ctcaaaattc
    15541 aataattgct ctttcttagc tccgccatga actctttcta gaaacccact attctccagc
    15601 tcttgtaaat cacgtcttat cgttgattca gaagcattaa aaaggtttac aagatcttgt
    15661 gatttcacga ttttctgctg ttcaagcaag tctaggattt tttgtctacg ttcctctgta
    15721 agcattttca cgatcacctc aatacaaata atagcatatt ttttttctac tcgcaatata
    15781 aatcagtcaa aaacgttcat aaaaattata aatcagtcaa aaaaaaataa aaaaccagtc
    15841 aaaaagactg gttttttatt tttttgtcta tcatcaagcc gttttatcgt tttttatttt
    15901 cgcttcttgt ttttttcata taaacagaag caggttttct ttccttatga agaagataat
    15961 aataaagtcc catcccgcca aacatgacca aaaaaccgat gactgatcga ataggaaaat
    16021 taggaacaaa aaagagacag acggataata atgatgccgg aaccattatt cgataataga
    16081 gttgtttatc cattttcttc acctcatatt ttttgtaaag taatcaattg ttctaattct
    16141 cggtaatcta aaaattgcac acgcagatgc tgctcacctt tcttgacatc gaattggata
    16201 cttcgccgct ttttctttat tcgtttgaag gtaacatcag agagtctatg tttgatttcc
    16261 tttaacggta attctgacag ggattctgaa ggaacataga ttcttaagga taaaagatac
    16321 tcttgatatt tttcatctgt tacctctact tctagatatt ccctaatata ataacaataa
    16381 gcttcgattc cattatccaa agcaacagtc ggatcgataa ctttatagca catcatgcgc
    16441 gagcgattct cgtcaaccca aggaaaatac gcctgagcat ttagtgttcc gcgaacacgc
    16501 ctttctttat aatctccttg ttgaatatca taactttttg ttacaaaaaa tgaagcattc
    16561 tttttaggaa caggttgcat ttgtgcattc tgatactgga cagattggat cgtatggtca
    16621 gtgtaaatgt attcaacgaa tggcggtttc aataaaccga caccattgtc ccaagcaaga
    16681 tgatcaaacc caagctggtc cctaaaatag gattgaaaca tttgattgtt tggcttttgc
    16741 cagttaaagg ggtctcgata aagctggata catatgactt cgttttctaa ttccttcact
    16801 ttttctactt gttggatgtc gagaaaaatc ggtttaggga tgatttgatg ataatagcga
    16861 ctataataca tttcccaaca cgaagtgaaa cataaacctt ggtaaaaaag atcgtatcct
    16921 ggaaattggt tgcaatcgac tactacttcc ccgttttgtt gtttcatttt aggtaaatgt
    16981 tcgatttcat cctttttctc aaacaatagc cgttgagaga tctgttttgt attgtgatag
    17041 ataaactcgc tataagctcg catataggcg aacaccccat gagcaatcat ttttttctga
    17101 atgaagtctc gaatcaaaaa ttgcttttct ttgaaaacag agatagacaa tacatttttt
    17161 atcaataatt gattatcttc caagcgaaaa ctacattcgt tctcgtcatt tcctacaact
    17221 aaccaacttt cgtctcttac cgatgctagc attatttttt cttgaagctt tcgaagatca
    17281 tacggatccg cttgttgtgt gaaagagcgg ataaatgttg gctggatcaa cagacgtgtc
    17341 aaaatagccc aatcgcttat ttcctgtttt ttttcttctt catttaagtc tgtgagctgg
    17401 atagtcatca ataatttatc ttcgccaatc attttctcac ctctttaaaa tctttttata
    17461 atttaattat aaccaacgat aaagaaaaac taaaacttta tgtcttagaa accaatttgg
    17521 acgattacat gatagactaa gtaaaaagaa acaaagaggt gtttacaatg gggttgaaat
    17581 ttgaacgtac agataattta gataaacttt tcgaatcatt tgctgtcgat ccggaaaaaa
    17641 cagttgcacc aaaagaagag atcgatcgat ttctaaaacc acagccacaa aatgaaaaga
    17701 aaaaagacaa gaaacaaaag taagttaagc tcaaaaaaag acttaccagc caaactt
//
LOCUS       DACCXO010000051        17374 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.052, whole genome shotgun sequence.
ACCESSION   DACCXO010000051 DACCXO010000000
VERSION     DACCXO010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.052"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(135..680)
                     /locus_tag="IVY70_001587"
     CDS             complement(135..680)
                     /locus_tag="IVY70_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7958720.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(692..1384)
                     /locus_tag="IVY70_001588"
     CDS             complement(692..1384)
                     /locus_tag="IVY70_001588"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP7958721.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1403..1687)
                     /locus_tag="IVY70_001589"
     CDS             complement(1403..1687)
                     /locus_tag="IVY70_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958722.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1700..2263)
                     /locus_tag="IVY70_001590"
     CDS             complement(1700..2263)
                     /locus_tag="IVY70_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP7958723.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2491..3153
                     /locus_tag="IVY70_001591"
     CDS             2491..3153
                     /locus_tag="IVY70_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP7958724.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3277..4473
                     /locus_tag="IVY70_001592"
     CDS             3277..4473
                     /locus_tag="IVY70_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP7958725.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4574..5950)
                     /locus_tag="IVY70_001593"
     CDS             complement(4574..5950)
                     /locus_tag="IVY70_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7958726.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5987..6853)
                     /locus_tag="IVY70_001594"
     CDS             complement(5987..6853)
                     /locus_tag="IVY70_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP7958727.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6942..7967)
                     /locus_tag="IVY70_001595"
     CDS             complement(6942..7967)
                     /locus_tag="IVY70_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7958728.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7985..8659)
                     /locus_tag="IVY70_001596"
     CDS             complement(7985..8659)
                     /locus_tag="IVY70_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7958729.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8843..9736)
                     /locus_tag="IVY70_001597"
     CDS             complement(8843..9736)
                     /locus_tag="IVY70_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7958730.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9810..11507)
                     /locus_tag="IVY70_001598"
     CDS             complement(9810..11507)
                     /locus_tag="IVY70_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7958731.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11500..12813)
                     /locus_tag="IVY70_001599"
     CDS             complement(11500..12813)
                     /locus_tag="IVY70_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7958732.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12813..16850)
                     /locus_tag="IVY70_001600"
     CDS             complement(12813..16850)
                     /locus_tag="IVY70_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP7958733.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16863..17321)
                     /locus_tag="IVY70_001601"
     CDS             complement(16863..17321)
                     /locus_tag="IVY70_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7958734.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4876 a         3896 c         3024 g         5578 t
ORIGIN      
        1 aagagattta ttaacggaaa aagaataagt ttttttaaaa taacgtataa aaaagagcat
       61 aaaacaaagt cgtttgtttt atgctcttct tttattattt tgtgcttttt attttatcaa
      121 atcactttgt ttttttataa aatttctgcg cctagtgtgg actcaaaatg tctcaatgcc
      181 cattcatgtc caacgggatc gaaacttgcc acggaatctt tgaaaatatg gagtttgtat
      241 cctagattat acgcatcgac tgctgtatgc aacacacaga tatctgtaca tacgccggtc
      301 aaataaatat ctgtgatctg gcgttctcgc aaacggatgt ctagatctgt tccactaaaa
      361 gcggaataat gccgtttgtc tatccaataa acattttcct cttgctcata gtcttggtat
      421 aaacttttta aagagccgta caattcccgt ccacccgttc ctatcacatt atgcggagga
      481 aataagcggt tctccggatg aaaggaatct tttggatcat gcgcatcaat ggcaaaaacg
      541 acaaaatcct tttgatcaat aaacttcttt gtatgagcag tcagcttttt ttcaattact
      601 tgtcctgctg tcccagtagt cagctttcca tcctctgcaa caaaatcata tgtgtagtca
      661 atcgagatca atgcgttcat cttagtcctc cttatacgag atgtttgaca aattcgatca
      721 ccatttctgc tgatcgtttg cctgcgtctt cgataaattc gtcaaagctt tgtgtcgccg
      781 agtgatctgc tgtgtcgctc attgcacgta cgatcaaaaa tgggatattg aactgacgag
      841 ctgtttgtcc aacagcggct ccttccattt cgcaagctaa tgcttcaggg aaattcgtta
      901 agatttcttt gattttatct ggcgagtcga caaatgtatc acctgtaacg atcaatcctt
      961 cttttgcgtt caaattcgtt gcttttgctg ctttaaccat ttcactgcgc aaatatgtgc
     1021 ttgcttcata atatagaggc attcctggta attgtcctgg gttgtagcca aatcctgttg
     1081 catctgcatc aaaataagct actttgtctg atataacgat gtcacctact tgtaagcctt
     1141 caccgattcc gccagctgaa cctgtattga tcaccatgtt cacgccgtat tgttggatca
     1201 acagacttgt ggtaatagat gctaagactt ttcctatacc ggaacgtacg acgatcactt
     1261 cgtggttacc taatgaacct gaaataaata aagcgcctgc gcgttcccat gataatggtt
     1321 cgcttaggtt ctctctcaaa atttttactt cttcttccat tgcgccgata ataccgattt
     1381 tcatcttttt aattccttct ttctatataa atgcgattgc taaaaaaacc acacataaca
     1441 ttaggataac aataataaga gatttcatca aaaaagagtt ccatttggtc gtcttttccc
     1501 gttcgctgat ccttgttttc gtgattggtt tattcttttt gctttcttta cgataaaagc
     1561 tggcaatctt tttttcttct tgctgataag ctgtttctgc ttttcgctgt ttttttaatg
     1621 actcgcttgc ttgtgcttgt tgtcgcttgc gaagttcgct tcgcgtaata agcggtcctt
     1681 tggccataag ccgatccctc tactttcctt tggtcaatag ttcccagtat tgaatcgcat
     1741 aaatcgtctt tgcatcacaa atcaactgat ctttcatcgc ttgttgcgcc tcttcaagag
     1801 ttagatgata caactccagt acttcgtctt catcttgtgc taaaggattc tcgaccttct
     1861 ctaccccttg tgcatgataa atatgaagga cttcatttgc aaaacctggt gaaagataca
     1921 tcgaagtaag atggctcagc gacttcgcac gatagccagt ttcttcttct aattctctag
     1981 ctgctgtcat ttcgggattt tgtccttctc ctggatcgat ttttccagct ggaatctcca
     2041 aaatcacttt ttctaaaggt tttcgaaatt gtttgacaag aagcagacga tcttgttcgt
     2101 caaaagcaat gatcccgaca ccgccaggat gaaataccag ttctctcttc gaagttcctc
     2161 catctggcaa acgaacttcg tctacagcaa catcaataat ttttccgcta tagatttctt
     2221 tgcgactgat tgttttttct tcaaattgct cattgttcag catatcttct cctgctttct
     2281 ttgtaatcat aactgctttt ttttcatttt tcaatttttc tcaaaaactc cttattagtt
     2341 taccatagat aaaccttaga ttttctctaa atatagaaaa tccttcttta gtccgatact
     2401 ctttctagta gtccgtatgc taaaataagt atgtaatcga ataacgaaaa gaattcaata
     2461 ctcaacgata aatgacatgg agggtttatt atgataaaac gaaattggcc gtggcttcta
     2521 gcattggtct tgctatttct ctttgtccga aacaatagtt tttcttttgt tctaggactt
     2581 ttattgatag gtgtagtttt agttttgtgg ggactctttg gttcgcgcag gaaaaaggga
     2641 aaacaagaag aaattcctgc actgactgac gaacttgaaa ctcactatga aaaaagcggt
     2701 atgacagcga atgaaatttc attttttcga caaacaatga atcaaacaaa aaatgagatc
     2761 aatcaattgc aaatgaatat gcagcagaca gccaaactta aagcaatcga tctccgccat
     2821 gatccagtca aagcagcgaa agctttattc aaagaattgg tgaaagaacc aaaccgactg
     2881 catgaagcaa gtcagtttct ttatacgcat ctgccgaatc tagttgattt gacaaataag
     2941 tataatgaga tcaatgacca tgagatcaaa aacaaacaga cctatgaaaa actagaagaa
     3001 agcgcgcaga tcatcgacca gctgtcagct ttaatcgcac aagattatca aaaattcgtt
     3061 gctgaggatc tagatgatat cgacgtagag atatctgttg cgaaacagag cttaaagcgt
     3121 gataataaat atgaaccaga acaaaaggat taactctttt gttcataaaa gaagactcgg
     3181 aacattcaga tgatgtctgg ctgtttcgag tactttttct aaagtagaga cattcatgta
     3241 agaatcgaga acagattcaa caaggaggaa ttttttatgg atcataaacc gactgaaaca
     3301 aaagatgtta caccagttaa cgatacactt gaagatttat tgaacaaccc gttttcaact
     3361 ccaacagata cgctgaccac ttcacaacaa gcggagattg atcagctcca agagcaacag
     3421 acagctgctc gtttaattga caaactaccg gcggaacgcc aagaacaagc taaacagcta
     3481 gcagcaaaaa tcgatgctaa tgatgcacag tctgtcatca gctacggatc ggctgcccaa
     3541 gcaaaattaa gtgaattctc acaatcgatg ttgaatcatg tgcaagcaca agatattggt
     3601 cctgtaggag attccttaac tgaactgatg tatcgtctcc aagaagccaa tcctgacgaa
     3661 ctgcgtgccg gagaagggaa catcttccag cgtgtcttcg ggaaggtcaa acaatcgatc
     3721 tatgaagtga cagcaaaata tcaaaagatt ggtgcacaaa ttgacaagat ttcagtgaaa
     3781 ttagataaag aaaaagacgg tctgctaaaa gataatctga tgttagaaca gctttaccag
     3841 aaaaacaaag actactttga tgcactgaat atttatatcg ctgctggaga attgaaaatg
     3901 gaagaacttc aaacaacgat cattccagaa gcgatgaaaa gagcagaaga aactggcgat
     3961 cagatggatg ttcagattgc caatgattac acgcaattcc tagatcgtct ggataaacgt
     4021 acgcatgatc ttcgcttagc tagacagatc acgatccagc aagcaccaca aatccgtttg
     4081 atccaaaaca caaatcaggc cttggctgaa aagatccaag catctatcgc tacagccatt
     4141 ccgctgtgga aaaaccaagt cgtgatcgca ttgaccttgc ttcgtcaaaa agatgcagta
     4201 actgctcaac gccaagtctc tgaaacaaca aacgacttat tgaagaaaaa ttcagaaatg
     4261 ctgaaaatct cagccatcga aacagcgaaa gaaaatgaac gtggaatcgt ggatatcgag
     4321 acccttcaaa ccactcaaaa tgatttgatc gaaacaatcc aagagacatt gcgtatccaa
     4381 aaagaaggaa aagaaaaacg tcgtcatgca gaagtagagc ttggacacat ggaagaagat
     4441 ctgaaacaaa aactgttgga tttaacccaa tagatcacct ttcgcttgtt actaaaacaa
     4501 acgcccaaca acagtttatt acaactgttg atgggcgttt ttcttatctt tcttctaact
     4561 tctccattgg agtctatttc tcattcatct tgctgtgtcc atagccatag ccaaaataaa
     4621 tgatcagtcc aataaccatt acgataccga acacgatcca tgtcacagtt tgcagacgaa
     4681 gcatcaatac aatacaagtc aagatggaga cgattggcaa tagcggaacg aatggcactt
     4741 tgaattctcc agcttttggt ttgccaaaca tttttctcaa cttcagtaag cctagactaa
     4801 caagcatcaa ataggcgagc gccatgatat tcgttagttc cgctaataca ttcaatggga
     4861 aaaatccggc aaagaataca gctgccacac cagcggcata agtcgctttt ttcggtgtac
     4921 gatgcttttg atcgatttct gtcataaact taggaagtaa gccatccttg ctgacagcaa
     4981 acagtaaacg agcaagtgaa tacatcatcg agatcgttac cgtcagtagc gttaaaattg
     5041 cccctacagc gatcaccgcg cccactgttc catgtcctac atacctcatt gcaaaagcca
     5101 ctgggtcatt gactcctagt tcagtatacg gaacgattcc tgtcaagatc agcgtcacga
     5161 tgatgtaaag gacagtagca atcagtatcg aaccaatgat tcctcttggc acatcttttt
     5221 gcgggttttt gacttcctcg gctgccattg caacggcatc aaatcccaag aaagcaaaaa
     5281 acacaagagc tgcaccatct agcacaccgg aaaaaccaaa aggcatgaat ggctgccagt
     5341 tatcaggttt aacgaaaaat attcctacaa tgataaacaa cgcgatgata ccaaatttga
     5401 catataccat ggcattattc aaacgtaatg ctttcttggc ttcaagcgac acgatgtaag
     5461 tgacgaatat cactacgagc accgcaatca agtcaatgta tgtaccattt tccggattgt
     5521 acccgccagt taatgcttgt ggaagttcgg cccctaaact tttcaaaaaa ccttgcatat
     5581 agccagacca tccggaagca acagaggaaa ccgctagtaa aaattccccg atcaacaacc
     5641 accctgtcag ccaggcagca aattcaccaa atacaacata cagataggca tatggcccac
     5701 caatcactgg cactcgcgaa gcaaattctg cgaaacttag tccagacaaa atgacaacga
     5761 ttgcagctaa gacaaatgaa agtgacagtg ctggaccagc attttgattg gccgcaaccc
     5821 ctgtcaccac aaatattccc gtaccgatga tcgcaccaat tccaagcatg atcaaatcag
     5881 acgtttttag ttcttttttc atctcactcg tatgattgag attgatctct ttcttacgaa
     5941 acaaatccat tgaagttcct ccccatttta ggttggaaaa cagcgattaa actgttttca
     6001 tataccctaa aaggatacca cctaaaatga ctaaaaggca accaacaata acataaatga
     6061 gctctttttt cgtttttctt tcgccaagga tgaaaatccc acctaaagta gagataatga
     6121 tccccatttg tgaaagtgag aagccaacag ctaaaccaag tgatttgact gttagaagca
     6181 tgcagatgtt tcctaagccc cataataatc cacagatcat gtttttccac acaaactgat
     6241 cgactttaac tttacggaaa gcaaagaagc ttgcgcctaa gatcattccg atactttgag
     6301 gtaagataat tgacatcgca tccaaattcg cccagttgac gatgatggta tatacaccat
     6361 aaccaatcgt agagaagatc aatgctctaa aacccttacc aaaatcaagc attttattat
     6421 ctgtatctat ttttccttcg tcatcttgtc gggcagtcaa atacgcaccg cttacaagaa
     6481 gagctaaagc aataataccg tatacataat ctttcgtttg tgtccattca tggaagaaaa
     6541 cggctcccgc aatcgtattc aaaattaatt gaaaaccagt agacaatggt agccctacag
     6601 aaacacccat gtacttcatt ccatggaact gtccattttg tccaacactc caagctaagc
     6661 cggataaaaa tccgatcaag aaaatccaag gggtgattac aggactaaca acgaaaaaga
     6721 caactaaaga aaacagaaat gcaccgatcg tcattcctag tgtttgttga ttggcatcgc
     6781 caccaatttt accacttact aaaccaatac taccccatgc caacataggg actaatgcta
     6841 ttaaaatttc cataaccaat ctccttttat ttcacatgat aagaaactta tcagcgaaaa
     6901 cgttaacaac acaagcactt tttttcgttt ctaacgattt tctaacgttt ttcattataa
     6961 aaatttaaga ttgggaaaaa taaagtcatt gttgtccctt ttccttcagt tgattcagcc
     7021 gtcaaatgga tacctaattt ttcggccaga ttttttgcta aataaagtcc taatcctgtt
     7081 gaatgtgttt tgctaagtcg accatttctc ccagtaaatc ccttatcaaa gatccgtttc
     7141 aagtcttccg ctgggatacc gatccctgaa tctttaactg aaaggctgac tccttgcgct
     7201 tccttttcta tgctcacaat gatgtttccg tgatccggcg tatatttgat ggcattacta
     7261 agcaattggt tgaagataaa agcgatccat ttagcatcgg tcaaaacagt atgatcctca
     7321 ccaacgatct cgtagcgaat atttttttgg ataaaataat ttgcctgact gcgtatcaca
     7381 ggctgaatga ttccttttag tccatattct tggatgaggt agtcccgcga gaaactgtcg
     7441 agtcttgcat aatacaaaac ttgttctacg tattcatcga tttttgataa ttcattttct
     7501 agtaatgtat acttttgatc gtcaatatcg aactcgatcg aacgaactag caatttagaa
     7561 gcggaaagtg gcactttgat ttcatggacc catgagtcaa tataatcttt ttggtcttgt
     7621 tgattactga ttgctgattg cattacttct tggtgttcac ggatcagctg attgatatat
     7681 tcttctacca gtttttcttc ttctgtcctc ccaccactca aataattttg caaaggggaa
     7741 gttttttgta tgtcggctaa ttttccccac catcttctct tgctcaagta accacccagc
     7801 agataaaaaa tcagaaagac gccctcaatc aaagctaaat aagcaattgt gctcaaatca
     7861 actttgatgt ttggcgccag ccacatcaca aagatcgtca atgcaataaa aaacagccat
     7921 cccaagatca atgtccactg atctttcaaa tatttaaaaa aattcatctt aaccgctcat
     7981 cctttcaggg aacgatatat ccctgtccta cttttgtttc gatatatcct tggatacctg
     8041 cctgttcgat ttttcggcgt aaccgattga tgttgactgt caatgtattg tcatctacaa
     8101 atcgttcgtc atcccacagt gctcgcagca atttttcccg acttaagatc tttccattgt
     8161 gcttcatcaa aataaataat agacgatatt catttttgct caaatcaatg atctctccgt
     8221 tgatttccat acttccgttg tcaacattca acgtaatgcc attgtggctc atcatttcac
     8281 tagataattc tgtgtagttg taagaacggc gaagtaatgc attgattttt gcaatcagta
     8341 catcgatttg gaaatgtttt gtcacaaaat catctgcccc catgttcata gccatgatca
     8401 tatccatatt cgtattcctg ctggagataa aaatgatcgg tactttcgat acttcacgta
     8461 ttttttgaca ccaataatag ccatccaaaa cgggaagatt aatatctagt aatacaagct
     8521 gtggttcttc tctttcaaag tctgagaaca cttgattgaa atcggtcgtt ccaaaggttt
     8581 caaattgcca tttctcaagt tcctccatga tcaattgtcg aatgatctct tcgtcttcga
     8641 ctaccatgat ttttgccata aaaaatccct ccatctttct tcttgctact acaatatcaa
     8701 agaagccctt gtgaaacaag ttggcttacc tggaaaatgt cattttagca aacgttaaaa
     8761 aaaactggaa atatgcgatt taatcgcgtt tgtctcttcc aagcagctta aatttattat
     8821 gttattctta ataattggtc tattatctgt tatttttctt tcgttgtttt tgtcttttaa
     8881 attttcgata acaccaataa ccgaaaagcg ttagcactag caatatacct agaaggagta
     8941 taagtaatcg ataaagatgg tatttttttg tctgttctat ctttgaagag acttcttctg
     9001 taaagggaac tcgtcttcct gtcactagta atcggtgagt gttcacggtg taaggtgtac
     9061 aagtgataag tgtgactaga tcctgctttt ctctaattgc taatgtattt gtttttgttg
     9121 gaaggacaac aattttctca acgacttcat aagcgagcat ccgaccatac acttcaagat
     9181 agaatcggtc atcttgctcc atttcttcaa gatccgtaaa gagttttttc tcagcaatac
     9241 cgctatgggc ggtaatcacg gaatgtgtac tctctcctcc agttggaaaa gaggtgcctt
     9301 gcaggactgt cgctcctcta tctaacaaaa gatcattcgt ttcatcaaat aaaggcaagc
     9361 taacattgat tttcggaata tagatggctc cgatcatatg ttgttcgtaa tattcttttc
     9421 ctggattttg gacatttcta accgcttgct caaaaggatc tttgacttgt cccattcctg
     9481 ggattactga tgtatgagca agcgtctgat ttttcttttc atgcactgct aattgttgct
     9541 catgtttttc ttctttatcg cgttcaagct gcttttgata attctcgatc cttctttgat
     9601 ccaaataatt gctgagagca tctgctaaaa atggatatgt aaaaactgca gctccggcaa
     9661 gaaacatcac tgccataaat aattgtaata aaagactgat ttgggtttga ggtctattac
     9721 ctattttctt tttcattttc ttcaccttct tatttaaaaa aactgcagca acctttataa
     9781 aaccgattgc tgcagcttat ttttgttgct tatacttcag acttcatttt agatttacgg
     9841 taccagctgt acgctccaat catcaaactt attccgatta gtaagaatgc caagagcccg
     9901 ttcccaccag tagcaggaag tgacccgcgt ttcatattcg gtactctttc tggccctgga
     9961 ttttctactc ctacgatctg aatttcttca ttatagcttc cataagagat gataaattca
    10021 gtaaaggctg cctcacctgt tggaagtaca taaccttctg gcgccatggt ctctttcatt
    10081 tgataggttc cataatctaa cccttgaacc tcaaagtatc catttccatc tgcgacaaat
    10141 tctgttgctt gtccgcttgt tgcccactca attctatctg ctcctgattc ataaacagct
    10201 aaaggcgact cccctttata aaatattgcg tattcagaac ggtcttcatt ccacaattcg
    10261 aatcttgcat ctttaagcaa ttcactgctt gatgcatcat atttttcaaa ctttcttcca
    10321 cctgttgtga ctttctctgg cggctctact ccttcttcat aatcaaattc atcatttcgt
    10381 ccgatttcaa taaccgctgt gttgttatgg aaatcatccg cttctagttc tgtggaaacc
    10441 ttcaaattat acgtgatcgt taaatctcct ccacgtgcat ctgctaatct acctaaagta
    10501 tctactttgg ctactccatt ttcttctgtc aaaatcgtca cgacaaaccc gttgcttact
    10561 acttctacta tataatcttg tccttttgtt aaaatcgtct cgccggctcg cacttctatt
    10621 tcctgatcat aataagttag accaactgta ggcgtatctg tgattttgta atttttgata
    10681 acagcgcttc cgttttcaag gacagaatcg atgaaataag gaataggtgc tgtgatcgtg
    10741 taagaagccg tttttcctct ttctagatca atataagaaa tcgtttcttg ttctccagct
    10801 tcattaataa tcgttacttg tctaaggtcg ctttcctcta cattcatctg tttatctgct
    10861 tctctgattt cgtttttcgg atacaaatgc acatctgtat tgtatgtgtc tttccatgta
    10921 gaaccatccc acacgccatc caccatatca ggcatcatca ctggcatcat aacaaccatt
    10981 ggagacgcga ttctggaaat atgagctggc gaataagttt ctaagaaaag atagacttta
    11041 tctctagaag aaggtgcttc ttccgtcact tggacggtag agaataccgc ttctccagct
    11101 tcattcgttg tttgtttgtc aatacttgtc agtcgatatt ttaaaaacca actttctgtg
    11161 tgccgttttt gaatccattc aatcgcttcg gcagaattaa gaccgtcttc tcgttccggt
    11221 gttaaaggat tatctcgaat caaatcgtaa tatacttccg ttacgtcaaa caaatcaaaa
    11281 tcgactcctg gaatattttc ttcaccaaaa tccgtagaaa ccaatccgct attcggtcgt
    11341 tcttccggaa cagatgagaa accttttttg tgtaatgtaa tcgtcacttc acttggtttt
    11401 tgtgcgactt cttcggcttt gactagtttt ccgttcccga aaatccctat tcccgcagta
    11461 acgagtaaca gtacactcat tcccgtgaca atcatttttt tattcatttt tttctccccg
    11521 ttcttttttt attaataaaa cagcacctaa caataaaaga attcctagta aagaaatcaa
    11581 cgttcttgct tcattcgttt gcgggaatac ggaccatttt cctgattgac ctggattgct
    11641 tggaccactc ggctctttcg gtttgtccgt tgtttctttt acggtctcgt tataaacacg
    11701 tggtgttgcg gattcatagg cagagactgg aactttcccg gattctaatt cagccatctc
    11761 ctgtccgtta actactacat attgtggttg gccttttgca tccacccaaa attcagggat
    11821 gatgacctcg attctcttac ttgcttcatc catctcgtaa ccttccacac cttttaattc
    11881 ttcaaaataa taggtgccag aaggcaagaa acgttgtcct gtcgtaacaa ggccctcttt
    11941 atccgaaatg aatttgctta catttttgtc attcaaagga tcattgtctg ctggtttgat
    12001 ccattgattt ttcaagtcat tagcaggagc catatctaaa taatatttcc ccccttctat
    12061 catctgatat agagcaaaaa cagcaccttc taatggtttt cctttttcag ttgttcctct
    12121 ttgacgtcca tacttgaaga agtatggatc tcttagataa ccgacatttt taggatagat
    12181 atgaatctct tgcagttcct cggttgattc tgtcgggtga taaattggca aaatagcagc
    12241 aattgggata gctgtctgat cgatatcaac atccaatcca acttctggat caatcgattc
    12301 ttcgaaaatc aaataaactg catcccgatc ttggtttttc ttaggtgctt tgatacgtgc
    12361 aatcccgtct cctaatccgc caaccgtatc tttcccagtt gttaccgaag tccctatttg
    12421 gacaagcctt tcttctgtga ttagctgacg gatattgttt ctgttcgttt cagcgatttg
    12481 ctggacgaat tctttaggag tcatgttttc agcagctttt ttttcatgat aataatcggt
    12541 catgtcgtaa atggcaaatg tcgctccatt tagtggcacc gtctgtgaaa tgatatctgc
    12601 tgtttcccct gcttcctcag agatagccaa gccatcattt tcatggtatt caaattgatc
    12661 gttataatcg atatctcgaa tcatccgttt atgcaacacg aattccacga catcttcccc
    12721 tgattcttct gccgacatcg catgtggaag aaggactgct gcaagcggta gtaaaacaag
    12781 taattgaagc atcaaacaca acagcttttt cattaccttg ctccctcctt tttagattgt
    12841 ctgacaaaca aaccataggc tgctcctgca agcgccatga agaatgcagc aagcaggata
    12901 tagaataatc gtccggatcc tcctgtagca ggcaacggat tttcaggatc gttcccgaca
    12961 ctaagctgta atctgattcg atttgtctcc aaaagttcat attctaactc ttgctgtaga
    13021 tcaggcgtct ctgcttcttc gtttggataa tacgaaatct caacttttag tgtacccgct
    13081 tcttttcctg gaactacccg atcattttct atgtcaggtt cttctcgata actaatttct
    13141 aaaataaagt aacctggaag ttttctatag ctgtcaggtg acttcacttc cttgatccga
    13201 tagacccctt ccaacaagtc tgaagcattg aatagggaag aatcgtttgg atctggtgcc
    13261 aagcctccct cgatgatttc atactctcct gcactatttt gtttctcaat tgaaaattct
    13321 gcgccttcaa gacgtcggct attcatactg tcaattttca aaagttccaa atcgattggc
    13381 gctagttcat cttctaaggt tacttgtagt acaccatcga tgatttcata gccttccggc
    13441 aagggagcag tttcgtcttc gatgaccgta tcttcaatga taaacttacc ttcttcattc
    13501 agtgcaaact ggaacacttg atcagatagc cgatacccat ttggtgcgac agattccgag
    13561 acctcatatt caccaataga cagatgcaga ggttggtcgt aatttaaatc gacatacttt
    13621 tctactccta cggcacgaga atagaagtat ccgatcccag gcaaacgttt cgattcttct
    13681 ggttctaacg gattaccagt gtatgctcga tacagcggga aggaagaatc ccccactttc
    13741 ctgatatcga aacctgcacc atctaatgta tggccttcat ggtctaattt tctaactcgg
    13801 atctcccgat aatcaaacgt attgtaagca gtgaatgcta cccataaacg atcctcttca
    13861 tctgttgtat acgtgagata ttgccaattt tcttcacctg tttgcttgag ttggatagtc
    13921 cctatagcat tatcgtaaag tcgcaagatc catgtgtcat tttcatcagg ttctttgaaa
    13981 ccttctgggt tctggctctc aatgattttg tattctgcat agttggtagt ttcttcacta
    14041 tccttataaa caaatgaatc tggctctccg attggatgaa taactttctc accatccacg
    14101 attacatatt tcccgtcttc atctactgaa tagaacgtga gtctcccttt ctcttcctct
    14161 acttcgttca caccagaaag ccctttcgct actggatatc cgatttccag tccgttacca
    14221 tctgtttcat aaagttcaaa ctctgcgcca ttgattggca tattactgcc ttcaattagt
    14281 ttattgatat tcatgtggat tggcttgaat tcatttcgca tttccagttt gacatacaac
    14341 tcatcatact ctgtgccttc tatgttaggc tcttgtacaa cagaaagctt gtgatattct
    14401 ccttcataat aggaattcgg atctttacct tccggtccgc cgactctcga accagatgta
    14461 tattcatcta caagctcgta gtgacctaac atctcatctt cgttggctgc atattttttg
    14521 atttggaatt gatacgtctc atctactaac tgataacgtg gtggtccagc gatctctctg
    14581 atccaaaata tgtcgctgac tgctccagtt tcatctccta aaatcggcat cttcgtttca
    14641 aagatcgcta ttccgtccgt ggtagtttta cttgtttcga ttggttcggc ttccccactc
    14701 tctggatttt ggtcatataa tgcaaattcg actccagcca acggctcgcc attttgaccc
    14761 ttcttgctga cttggaaatt acggccttta tgttcgtaac gaatggaaga agatgggaaa
    14821 tacatccgtc cagtagtgat atcattctca taatcctcca ggttctgaat ataggccata
    14881 ttgttcactg tgcaaaaatt tccgtcttga gctcccgcat caagttttac ttggtacttg
    14941 atcctgacca tttccccttc acctaaagaa atcggagaaa atttataagc ttggttttcc
    15001 cgtccgacat acgttgtcgg agtcgccact acttctggtg gggcttgtcc atgacgttct
    15061 tcatcccata tttctggctc ttggtacttc atgccgtttt ctcctggatc tagatggtaa
    15121 aactgaatat ctgccgatct gccagctcca aatggtgaga cattaaaacc agtttcatca
    15181 taaagtgtgg cttgctggat cgttctcttg aaggtagaag agatatcctc caaggctgta
    15241 acaatgtcat tcttgttgtt cgcattgtaa tatcgcggtg ttacgccatc ttcttctaaa
    15301 tcagcaatat ttttcaatac atttcgtcca atagcatcta gtcgttgtct cccgctaacg
    15361 tttccatcga ttcctaaacc aatggagaag atacttggcg ggttctcttg atacttgttt
    15421 ttcagccaat gatgataagc aactgtgttg atcgcagatg acttttcttg cgaagtagga
    15481 ctgccattcg tattgttatt agaagaacca tcaccaaccc agtgagtcgc tttattatct
    15541 ccttctttta cctctccata acgatagtgt acatttactt catcttctgg acggttaaag
    15601 tcagatgcat aggtaactgg atgagtaaac cctgcattag aagtattccc agaaaactcc
    15661 tcaaaatttc tgaacaaaac gtcattatct tcagcaatct tatcttccat cacagtccag
    15721 ttgctccaag ctccattagt gctcgtacga taacttgact gaatcggtgc ataactgaag
    15781 gtcggtgtcc catctcccac aacgattagg attttttctg gatttctatt ttctccgcca
    15841 ttgtctttat acaatgtttc atagccattt ttaagcccta gtgtaagcgg agttcctccg
    15901 attggtgtac gatttaatgc tgtattctca cttcccgtca aacgattgat atcatttgtc
    15961 aatggaaaga tgtccgaact gatttgctca tggttattga tagctgtact attatggtaa
    16021 aaattcacca taccgattcg aatatccaga ctcgggtcag agagcagatc tttactcatt
    16081 tcgtttactg cctcaatcaa cgcagcattt ttagtttgac tattggtccc agcggttaat
    16141 tcactcatac tcgctgactt atctagaaca aaaacgatat cgattggact gatttccttt
    16201 ttctcgcccc cgatgacatc tagatagaca ttgtaaaggg ttgttgcatc ttcaggatgt
    16261 agatcagatg ctgtgctgct tggcagaaca ggttctaccc attttttgat gtacgccccg
    16321 ttataatcca agtaactgtt ttcaaaattc cgttcatttc cccaaagctg aacggtttga
    16381 gctggaacgt ttctatcttc ttcgcctgca gttgatccat tattagtagc atagtcgtaa
    16441 tttcgccagt tcgctccccc gtttgtataa tcactatcat cgatcaagtt ctctccgtca
    16501 tatggcgagc ctttgtctcc ttgatagcgt tcattgcttt gtgtccaatt tcctaacact
    16561 tttgtatagt cattcgttgt aaacttcggg tagcgatgct gttcgttttc aacttctccg
    16621 ctaggataga tatattcata ggggtcttgc gtagctgttg gcccaagacg ctccaaaata
    16681 ccgttgaata actggcgttg aatgcgctgg agtgcttgac cgctgggtac gattggttcg
    16741 tccagctttt ccatctcatt ttcttcgcta cgttgaattt cagcggaatc ttctgtatca
    16801 gtttcatttt cttgtaattc ttggttatct attctttcat caaccaccac ttgaagaggt
    16861 agttaccctt cgttcacatc ggggatcttt tctgtttcaa aaatcaatgt gccggtttct
    16921 gcttcttttg aataaacttt tccgacatac cattcttgac catcgatcgt ctcgactttt
    16981 aggtcttctc catcttcatc ataggaggat agacctgttc cacgaacgtt ttttacggta
    17041 ccaatcccgg cggctgccgc atcgattgct aaacgtattc taccttcatt ggcactttcc
    17101 tttttttcaa aagccagatg ccattcaagg gtttcttctt tttcaatata cttcccttca
    17161 atttttgcat agtcctcatc taagaatacc tcatcctcct ttgtttctgt tgactcagca
    17221 gctacatcca gggtcgtcat atgatagact gactggaaat atggtccaaa tagcaaactg
    17281 acagttacta actgcaaaag cttcaatttt aaccctttca catccttata ctcctttcaa
    17341 aaatatttgt cttctttcac aacaatcaac aaaa
//
LOCUS       DACCXO010000052        17371 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.053, whole genome shotgun sequence.
ACCESSION   DACCXO010000052 DACCXO010000000
VERSION     DACCXO010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.053"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            66..503
                     /locus_tag="IVY70_001602"
     CDS             66..503
                     /locus_tag="IVY70_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958735.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            598..1482
                     /locus_tag="IVY70_001603"
     CDS             598..1482
                     /locus_tag="IVY70_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP7958736.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1706..4873
                     /locus_tag="IVY70_001604"
     CDS             1706..4873
                     /locus_tag="IVY70_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958737.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4893..5438
                     /locus_tag="IVY70_001605"
     CDS             4893..5438
                     /locus_tag="IVY70_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAP7958738.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            5466..5921
                     /locus_tag="IVY70_001606"
     CDS             5466..5921
                     /locus_tag="IVY70_001606"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAP7958739.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            5918..6676
                     /locus_tag="IVY70_001607"
     CDS             5918..6676
                     /locus_tag="IVY70_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7958740.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6762..7502)
                     /locus_tag="IVY70_001608"
     CDS             complement(6762..7502)
                     /locus_tag="IVY70_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7958741.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7563..8816
                     /locus_tag="IVY70_001609"
     CDS             7563..8816
                     /locus_tag="IVY70_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7958742.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9138..9365
                     /locus_tag="IVY70_001610"
     CDS             9138..9365
                     /locus_tag="IVY70_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP7958743.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9488..9814
                     /locus_tag="IVY70_001611"
     CDS             9488..9814
                     /locus_tag="IVY70_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP7958744.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9882..10595
                     /locus_tag="IVY70_001612"
     CDS             9882..10595
                     /locus_tag="IVY70_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP7958745.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10905..11755
                     /locus_tag="IVY70_001613"
                     /pseudo
     CDS             10905..11755
                     /locus_tag="IVY70_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            11876..13741
                     /locus_tag="IVY70_001614"
     CDS             11876..13741
                     /locus_tag="IVY70_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7958746.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13789..15198
                     /locus_tag="IVY70_001615"
     CDS             13789..15198
                     /locus_tag="IVY70_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP7958747.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            15306..16007
                     /locus_tag="IVY70_001616"
     CDS             15306..16007
                     /locus_tag="IVY70_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7958748.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16166..16645
                     /locus_tag="IVY70_001617"
                     /pseudo
     CDS             16166..16645
                     /locus_tag="IVY70_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         5801 a         2912 c         3728 g         4930 t
ORIGIN      
        1 gatatttttc tgtataatct taaataaaga aaacaatatc aaaaaatgca cgtagaagta
       61 gataaatgga gtggttttgc aaatttcttt tatttctaga agttattcac gtatacggat
      121 ttgtttttct cacaaataaa aaggcaggaa gtacaatgga tcatttgtat ataaaatata
      181 ttcatcaatt ttccgttttg gtgttaggca cactaattat agcggggatt gtcgcattta
      241 ttattcaagg ccgtgacata ttaacagtac atcaagagtt tttcagttat gcatttggta
      301 ttcctctgat cgctataagg tctgataaca aaaagtggaa gaaacgctat tacattctct
      361 tgctattttt gtgtttttat ctgccaccta agatttttac tttattaggt atacaatgcc
      421 tatcattttc ttttatttcc tatatcattg ttttaatttt cctcagtttc atagtatgga
      481 tgttttatct tgtcaaaaaa taaatgaatg tttcatgaac agtacttcat cacagcctaa
      541 tttttttctt tatgctatcg ttatattaaa gaaatcaatc agaagggaaa gaaagtgatg
      601 aaaaaagttt tattagtggt aaaccccagt tcaggcggtg aacaagcaaa ggaatttgag
      661 caattagcga tagcaaaatt ggaatcggtg tttgatgaag tggtcgtctt gcatacaaaa
      721 aaagcagggg atgcaaaaaa ctttactcgc gaagcggcta cggaagggta tcatagtgtg
      781 tttgtcatgg gcggagatgg aacagtcaac gaaggaatca gtggaatcgc tgaacaagaa
      841 catcggccaa atttcggatt tttcccattg ggtacggtga atgacttggc tcgagcatta
      901 gggataccgc tagaacctga ggaagcgatc aatcattttt cgattgaatc agtcaagcca
      961 ttggatattg gaaaaataaa tgacgactat tttatgaatg tcgtcgcaat tggctctatc
     1021 cctgaatcaa tcaatgatgt agatgcggag aagaagacga aatttggaaa actagcttat
     1081 ttcatgtcag gaatcaaaca gttagcttcc acacaatcct actcgtttca cgtagaagtt
     1141 gatggaaaga aggaagaaat tgagagcagt accttgctga taggactaac taactcagtc
     1201 ggcggttttg aaacattact tccaaatgca aaagtggatg atggtaagct ccatttggtt
     1261 tatttaaaag attcttcttt gatcgatacg cttaaaacat tgccagacct attaaaagga
     1321 gtagatgaat ctacgaaaaa tctagcctac cttacttgtg agcaattagt tgtgtcatta
     1381 gctgatgagg aagaattgac gacgaatgtt gatggagatg aaggggataa attacctgtg
     1441 acaatcaaag tattgccctc acatttacag gtttattgtt aaaatggagg ttgtgaccga
     1501 ggagttggcg ggagaacacc gcttgttcgg gataagggac tgtgacaaga cttttgtcac
     1561 agcctttttt ttgtacaaaa aagaaattta cacctaatag aaaaattttt ccataaaaat
     1621 acatatttta aatatgaaag tacaattgga aatagattaa aacgataaaa taaaattgtc
     1681 aaaacgtcag agaggatggt ggagaatgaa tcaatgtgta aaaaagctga ttgcgtcgat
     1741 tttgtttgtc ttccctctct tgatggtgac atccgttttt gccgatgtag agaaaacaaa
     1801 cgaatcaacg caatcagaga atcaaagttt tgacaagact actttttcag aagaatcgat
     1861 tacttctgaa actacagaat ctcaaagtga ggcttctact aataaaaggc aagaagaaaa
     1921 taagacaagt gagacctcag aagtagctga acaaacgtct gatagcagtg aagcggcact
     1981 ggcaagagct gtttcagcgg atgggtacag cgaagctgcc acaacagaag aactggcgca
     2041 gcttctcgca gacgaatcag taactaagat ccgactgatt cagcctttga ctttagacag
     2101 ggaattagag atcaaacgag atatcgttat tgattttggt ggattcgctc acaatttcgg
     2161 cacacaccat atttatatca atgagatacc tcagtcgatc gagttccaga attttaaagg
     2221 aacagctgca catccaggtg gattgacacc aaatatcgat ggaaatgcca ttatcattgc
     2281 ttatatgacc gatttttggg gaagcagata tcattttacg ggagaaataa aatttaccgg
     2341 gacacttgat ttgtatgatg ggtccaaatt aggactgatt tacgctcctc gtgcaactgt
     2401 cacattagat ggtgtttccg gtgtgttgga tgtccaacca gtgaaggaag gactgaatgc
     2461 cgctccgggt aaagcctatt tttgtagaag ctatcaattg aatgtgatca atggtagtca
     2521 actttatgga ccgtatctag ggaaattcta cggttttcta gattcaggag atgagactgg
     2581 tagtggaaaa acagcgccag gaatccatat cttaagcggt tcaaaggtct ctcttgatta
     2641 tgatcgggca gatggcgtta gcgatgaagg agaggcagtc gatacgctac ctggtaatgt
     2701 catgtttgaa gtcagtggtt cgggaagcga attttcggta aatacaaaga tcaatattac
     2761 caatgacaac aatcgcggaa tcatccaaat gagaggttca ggatcgaaag tcactgtatc
     2821 gaatggtggg aaaatcacca tcaatacagc aactacgggc ggatttcgct tgcaaggaga
     2881 ttctagtcaa atccatgtat caagtggtgg acaaatctcg gtcaaaatgg ctggagatga
     2941 cgatcaaccc gggaacaatg ggatgcgttt tgtaggaaaa ggcctttctt tgatcgtaga
     3001 aggagccgga tcagttatcg acatcgataa gcagagtggg cgttccagcg cagttcgttt
     3061 tgagaatggt acgcaaaagc tgacagtaac caatggaggc tcattgaggg taagaaatac
     3121 aggtgatggc aaatcttacg tgaacagact caatcaagga aatcaagcga tccaatttta
     3181 tgacgcttct ggttttctag aaagtccagg ttcagctgat tttacagtca ctggagaaaa
     3241 atcaaatatc gatatacgcg cagattccgg agcaactgta gatacaactg gagatattga
     3301 tttaaatttt acggcaggtg aaaaaactta tttagtcatg gtcgggaaaa caggggaaga
     3361 taagaccggc atcatcagtg gggatgtgtt gaatgtggac ttagaatctc ctacctattt
     3421 tgatttccaa aacaaacgga ttggaagcga gaccaatggc ataggcggct gggtattcca
     3481 aggaaatgga gattcttctt taaagatcgc ccattcagaa atcgctttat ggcgaaaaac
     3541 aggcgcaaca ttcgataact ttgaaaaaga cccggattat ttttctgata tgacagactt
     3601 atcgatcaat gggaccgact taggcaattt cgtatcaagc cctagtgcag gtttaacaaa
     3661 tgaatttgct cagacaggtg ccggaatgaa aaattataat cgaatcagtg ccaataatca
     3721 gctcccgatg attgaatcct taagagtgcc aacgaatgca gataaaaaaa tctatgggca
     3781 tgtcgttgtt cccgaaggtg tcgaggcaaa gccaagagat gcttggacag gcgaagtaga
     3841 agtgacttta cgtattactt acgcagatac gacgaaatca tctgtagaaa ttactgctct
     3901 cacgcaagga caaacagatg aaaatgaagg ggcaggatat aacccatggg gagaaggcga
     3961 acaaggcggc ttgttcgaag ttcaagtacc caatgatgag agattaaaaa cggaggatca
     4021 agtgcaagtt gttgctgcta aaaaagtaag aggtggccaa gaagtgacag aactggactc
     4081 tgccaaaacg acagtcgatg tgattcctcc tgaacctgct aatttaagta ccacattgat
     4141 caatgcagct tctcgcacga tcacaggtac aggtgaagca ggagccttgg tttcattgaa
     4201 aaaaaatcaa gctgttttgt cagaaacaac tattgatgat acaggggaat ttgttctttc
     4261 tatcccagaa aatcaacttt ttgcaggaga tgtattagaa attgtattaa acgaccaagc
     4321 aggagaagct actgctaagg gtgtgcagaa caagccaagt accaacgatg aaattgggaa
     4381 tcacaatcca agtgatacgc cagttgttta tcatgatgcc ccgccttttg cacctgctac
     4441 gaagatcgta gtagaaggtg gtctctcatt tcttgcccct tccaaactcg attttggtca
     4501 gatcaaagca actggtttga cacaagaagc atttggccaa atggatatac aggaaaaatt
     4561 atggatttat gatcagcgag aaacgaaaaa tccgtggatc ttaacgataa aattatctca
     4621 gcctttcaca aatgatgcag gattttctat gcaggatgtc ctatatttca agaaaggaga
     4681 ggagtatcaa ttagtgaatg aagaagaact ggaaattgtc agaggacaaa attcaacgaa
     4741 tgtgcaagca gattattcag aatatttaaa taacggtcgg gcgtttaaac ttgtcttaac
     4801 caaagaaaat cagatagctg gtaattactc agcagagata acatggacat tagcagatgc
     4861 gccgacagaa taaagatcaa ggaggatgat tgatgaatcg aatatggaat aaaagtcatc
     4921 gaaacaactg ctctttactt atgatccttc tccttggatt catcctttgg cagacacctg
     4981 tccatgcctc cgaattcaat tttgcggtag attttgaatt accggctaat cagattgata
     5041 agaacaaaac atatataaat gtaaggatgg agcctgggaa aacacaggaa atcccctaca
     5101 cggtctttaa ccgaacagat aaatcagtaa cagttgagac attgatccac tccgctacga
     5161 cgaattcaaa cggtgtgatc gaatatggag aatcaaaaag taaatttgat gaaacattaa
     5221 gttatgatat gaaaaactta gcaaaaacag tgggagaaat aactgttcct cctcaaagtg
     5281 cggtcactga gacactttca ctgaaagcgc cagaaaaaga gctggctggt gtggtcgcag
     5341 gagggatcac gtttcaagaa aaaaaagaga acgtttctaa caaaagtaca gttggtgtcg
     5401 tcaatcagtt ttctcaagtg aaagcaatct tgatttgaaa tagcgaaaaa gagtcttccc
     5461 cagatttgaa attaaaaaaa gcttatgcgg atcaaagcga tggaagaaat gtgattaagg
     5521 cgaatcttca aaatccaaat gcttcttata tgttcggctt gactacatcg gttgaaatct
     5581 acaaaaaaga aacaaaagag ccttaccttt caataaaaaa agaaaaaata gatgtagcac
     5641 ccaatacaaa ttttgattta cttgtcccat tagaaggaaa aaaattagct ccgggacatt
     5701 atcaagcggt tgtgaagggt gcttggaaag aaaaacgctg gaatctccat accgattttg
     5761 aaataactga tgatgcagcg gaccaattga atgaaaagga tattgattta aaaaatcagc
     5821 cagaaacatt tgactggcga tggctgatac ttgttggagt cattcttatt ggcttatttg
     5881 ctggattatt tattttaaaa gaaagaagga aaaagaaatg aaaaaaacaa ttgcattaag
     5941 taccgttcta gctggaagtc tattattatg tacacaaagt gtttttgctg agaataagag
     6001 ctcagccact acagaaggaa cgatttctgt gaccaaacca gatgctgcaa atcctgaaga
     6061 cgttgttcca gttcctcctg ttgatccaaa tgatcctgat actcctttta cacctgacga
     6121 accaaatcag ggaacatccg gattactgac gattgaccaa gctccagatc ttgattttgg
     6181 aacaatcaag ttaggtacag caaaaacagt atatgctgca ttacaaagcg gaacggacag
     6241 tactggagca caaacacaag tacagaattt cgtacaagta acggataaaa gcggaaatta
     6301 tgccggttgg aaattatcag tcaaacgaac agaatttatt ggtacaacat ctgatacgaa
     6361 taaactaact ggttcaaagg tgaaattcaa aaacgccgtg attcgaaata gcgcaactaa
     6421 tagcagtgga atgccatcta ccgtttctgg aactggcgct gaaggaatcg aaatcccagc
     6481 agatcaggca gtagatctag taactgcagg agcaaatgaa gggattggca catggattta
     6541 ttcattagga gagaatgtgg aacaagggag acaatcagtg gaattatttg ttcctgtatc
     6601 caattatgct gtagacaact acgtttcaac attcacatgg tcgttaacag atgctcctac
     6661 agaagaggaa gcgtaaaatc atatcaacga tttcagggag tggagccgat tcaaggccca
     6721 ctccctgaaa tctgctatgc ttacgtctca gctcttattc atcaactcga agcagttttt
     6781 tggaagtcat ggaaacgttc ctttgtcttt tcaaagatct ttcgaccaac gagtaaggag
     6841 tcaccgttga aaaattctaa cacataatga gcagaatcaa ttttctgcaa ttgcgataag
     6901 ttgatacagt aagattttaa aagtaaaaag taagcatact tttctaactc tgtcttcaat
     6961 tgttttaaag aagtattcac taatttatct tctgtagttg ttttgaccat aacttggttt
     7021 ttcaaacctt tgatactttc aatatataaa atttcccgag gatacaggac gagacttcca
     7081 cctttttgat caataaagat tttatcgaca tttacgatct gctgttcttt ccaattgatc
     7141 tgttctagca cttgacgaat ttgttcagat aaattcctgt cttttctgat atatccaatc
     7201 ggaaggattt tccgattgat gatctctact gcaaaattcg tataagcggt gaaaaaaaca
     7261 atattactat gtgcattttt tttacgtatt tgttcggcga catcaatacc tgtgagtgat
     7321 tgcttcaagt cgatatcgat aaagaaaaca tcgtgattat caatagtgcc caactggatc
     7381 aactgattga tttgagagat agaagtgagc tgcacgatat tctcgatatg atgattgacc
     7441 aattctctga tgatccattc gctgtgaaac tggttatctt ctatgataaa atactttttc
     7501 atatgcaggc tcctattaaa aaaatatgtg tacattttga aacaaaacaa tggaggaata
     7561 agatggtcgc aacagtgaca ctttatttga cctttacttt gattttagat ttaggtatct
     7621 atttattctt ttcgaaacaa ctgctagtaa aaaacagctt tggttgtata gctttgatgg
     7681 tggtgacact tttacttcct atccctaaag tgagctttcc ttacctagtt ggcttattat
     7741 tgatgtttct tttttatttt actacgaaaa acctgtttat cagctgtttg atcagtaatc
     7801 ttttttttgc tatcactgga ctcagctttt ttctggtttt tgatctgatg gattatttag
     7861 gaaagagaca tcttggaaca gcaagttttt ttgcactact tatcgcattt attttctatc
     7921 tattagtaaa aagtatagac aaacagacga gatttctcaa cttgcttttt acttatccaa
     7981 ctgaacacta ttttggaggt atttgtattt tattaagctg gggcgtgatt tacttggtat
     8041 tgggaagtgt tcacttccgc tcatctaatt atctgcttct atctcttttg tcgatagtca
     8101 tcagtttttt ttctttgttt ttttccatta tgttgatcag tttccaagta aaagaaagac
     8161 aacatcagga atcgcttcaa ctgtatcaaa taaatgaaga atattaccat catctagagg
     8221 aattcaaaca tgattataaa agtctactgt tttcattaaa aattactctt caggaaaata
     8281 aaaaagaaac gttggaattt ttagaaatat tagaagatca ttctgcagct atttttgaaa
     8341 aaccgcaaat acaacagcta acaaatattg tatccccagc tgtcaggggt gtctttttaa
     8401 aatttctaga aaaagcagaa tcggagaaaa tcccgatcca agtagttatt cctgtcgaag
     8461 tgaaaacaat cccgattgat ctggttgttt ttattcgctg tttatctatt tttatttcta
     8521 acgcattcga tcatcgtgta gccgatcaga gtccgattac gatcaatctg ctggaagaaa
     8581 aagagggatc acgttttagt atagcgaaca aggcaaatcc aacgaatttg acgctatcgg
     8641 agatggtcaa aagaggaaaa acctctaaaa aaaatggcgg gttaggtcta tacagtgcga
     8701 aaaaaatgtt ggatgtttac gaaaatgcag aactaaaaat cgaattttca aaacagaatc
     8761 actatttttt tgtagaaatg tatctggcat ctagaaagag caaatcagcc tactgactta
     8821 ctgtcaaagg ataagtttga ggaaagtcta cacggagata gagacaacaa cacagcatat
     8881 tcaaaagaaa cagaagcgtg atgaccgttg tcacccttct gtttcttttg ctatgtagtt
     8941 aaacaaagta gttgacactt ccgctactta gtattagtac gaaagtatac ttcatgatac
     9001 taagtagata agaagtgaaa agcggaaccc acgatcgtca aaatgctaac tgctttgctt
     9061 acaccagata aagggaagag caatatcgct tattttaggc gtggagaagc aagcaaatcc
     9121 ctacgatcac aaaaaaaatg ctgacccagc agttaagaga gttagaacaa gatgaaagta
     9181 tccatcgaaa agcctataac caagtcccgc ctaaagtaga gtactcgctc accgaagaag
     9241 gaaaatcatt aagagagatt ctagtagcgt tgtctgtttg ggaagaagaa cgtatcgaaa
     9301 aacaaaaagc ctcaagggaa gaagacacgc tactgcatac acatggtggc tacctaaatt
     9361 attgattttc tatgacattt attttaaata aaagaaaaca ggagtttttt taaaagcatc
     9421 atctattttt tatgtaaaaa aatggtatga ttaatcaagt aagaagtgat tggaagaagg
     9481 caagcaaatg aatagtatgg aaaaagtcaa ccagtttaga gatgaacgta attggcgaca
     9541 atttcataac gaaaaagatt tggctatctc tatttctttg gaagcatctg aattgttaga
     9601 actttttcaa tggaaaacgc cggaagaagt aaaagaaaca caattagaac gaatcaaaga
     9661 agaactggca gatgtattga tctattcata catgatggca gacaaccttc acctggatct
     9721 agatgagatc attgaagaaa aattgatcaa aaataatttg aaatatcctg tagaaaaaag
     9781 cgcaggtgta cgaaaaaaat acacagagtt gtagaaatgt gtcattcggc tgttggaaag
     9841 agcaaaagaa aaacagtaaa atgagtctgg agggagagaa aatgaaaaaa aaaggaaaag
     9901 caaaaagatg gctagtcaat atcctcttgt tcctcttgtt attagtagga ctagctttga
     9961 ttttcaatga acagatcaag gattattttg tcagagagac aggagataaa tatgctatag
    10021 cgaatgtaac aaaagaagat ctgaaaaaga ataacgataa agatgtcagt tttgactttg
    10081 atgcggtaga accaatgacg acagaagggg tcatgaggtc gcaaatgaga ggaacagatc
    10141 taccagtgat tgctagtatc gcagtccctt cagtttcaat caatttaccg gttttcaaag
    10201 gcttggataa tacgtcacta ctttacggag caggaacatt atctccagat caagaaatgg
    10261 gaaaaggaaa ttatgcctta gcaagccatc gtgcaacgaa ccctgaattg ctgtttacac
    10321 cgctagaaaa tttagagatg ggtgccaaga tctatttgac agatttagaa aatgtgtata
    10381 catacaaaac attttttaaa gaaaaagttg ctcctacaga tacccaatta ctgaatgaag
    10441 tcgagggaaa agaaattgtc acactaatta cttgtggcga catggacgcc gtcacgcgac
    10501 tagttgtaca aggagaactg gaaagtgtta catcgataaa agatgcgaca gatgatatga
    10561 gatctgcttt caatctagag acaaaaacat tttaggatac tgggacataa gtcgttacag
    10621 attaaaaaca gccaaaggat atgaatcgat aaggaaaaac caatttgctg aaaaaaagag
    10681 aaacgcaaag aggtgcatct ctgatttggt tgttttctag atagacttgt gttccagttt
    10741 ttttattttt tatatgaaaa ggcttgcata aataaaaatt cgatggtatg atgattttga
    10801 ataagaaatg gattgttact gtacggcagg caaaaccaga attcagctaa gaaacagtag
    10861 gcgaattcta ggttttttta tttttcttga gagggcgaag caaaatgaaa attgataaaa
    10921 ttttgaataa caatgtcgtc atttcgaaaa atggattcgg ggaagaagtc gtttgtatgg
    10981 gaaaaggact agcttttcaa aaaaagattg gggacgagat ttcgccagaa gcggtccaaa
    11041 aagaatttgt ttaagagatt cttttgctaa aaaccaattc cagcagctga tggctgatat
    11101 tccggcagag gagatcgaat gggtcaaaca agtagtcgaa ctggctgaag ataaactaaa
    11161 agttgaattt tcgcctaata tttatctgac attgaccgat catcttcatt atgcgatcac
    11221 acgagcaaaa gaaaatatcc aactgcctaa tccattatta tttgaaacaa aaaaatttta
    11281 tccgaaagaa tatcaagcag cgaaagaggc tcttttgttc gtgaaacaaa agacgggtgt
    11341 ggaactttct gaggatgaag ccggttttat tgcctttcat tttgtgaata gccaacaagg
    11401 aaacgaagac atgcaagtga cgatgaccgc taccacaatg gtaagagata tactgagtat
    11461 catcagtaaa ttcttcggca tcgtgttcga tgaagaatct ttgaattatc agcgtatcat
    11521 tactcatctg cagttttttg cccagcgtta tctcaaaggg gaaggatctg acgagcaaga
    11581 tgaatttctc tacgcattag ttcaaggaaa gtatccaaaa gcgtttcatt gtgtcgaacg
    11641 gatcaatgag tatcttcgac aaacgcaaaa acaggaaatg gggatcgcag agcagattta
    11701 tttaacgatt catattcaaa gagtagtaag tgagaaaaaa agcatccaat cataaggatt
    11761 gttactgtat ggcaggcaaa acctggattg aaaaaatagg ttcacgaatg tttgtgactg
    11821 tttttttcaa tctgggtttt tattttttaa ataaaaaaga aagaaggaaa aaacaatgga
    11881 taatcaagca gttggtcgtc gtgtatggga agcggtagga ggacaaaaga atgtcaaaag
    11941 tttagtacat tgtgctacgc ggttgcgatt cagattaaaa gatgaatctt tagcagatac
    12001 gcaaaagtta aaagaagacc cggacgtgat ccaagtcgta caaagtggcg gtcagtatca
    12061 agtagtgatt ggcagtaatg tagcagatgt ttatcaatcg atcgtggatg aggaaggtct
    12121 tgcagtttcg gaggatggaa aagaagagtc caaaaatatt ttgaatcgtc tgatcgatat
    12181 tatctctagt atatttacac cttttctagg ggcgatggca gccgccgggg tattgaaagg
    12241 attcttatca ttggcaacgg tgatgggctg gctgacagct gatagcggta cttatcaaat
    12301 attgtttgct gcagcagatg gtgtctttac cttcttacct gtcatgcttg cttttacagc
    12361 agctaaaaag ttcaaagcca accagttttt agcagtagca attgctatgg cacttgtgta
    12421 tccagctatc acagcagttg ctggtgcggg agaagctatc agtttctttg ggatcccagt
    12481 gattctttca ccatctggtt atacttcttc tgtcatacca atcattctag ccgtatgggt
    12541 tcaaagtaaa ttagagccat tcgtaaaaaa agtgattccg caatttctgc aaatgatttt
    12601 agttccgctt gtcgttttag tagtgatggt tccattaaca tttcttgctt taggaccaat
    12661 cgggacggtt gccggtaatg ctttaggcgg tctcttcaac tcgatttatg gatttagtcc
    12721 aatcgtagca ggtctaatca tgggaagttt atggcaagta ttcgtgatgt tcggcatgca
    12781 ttggggattc gtaccgatca tgtttttgaa tatcgaacaa tatgggtttg atgtattgat
    12841 gccaatgctt cttccagcca tcttggcaca aggtggggct gctttagctg tagcattgcg
    12901 aacaaaagat acgaaacttc gagcattagg gatttcttct acagtgactt cgttatttgg
    12961 aatcactgaa ccaactgtgt atggtgtgac attacctttg aagaaaccat tcattgccgc
    13021 ttgtatttct ggaggtattg gtggagctat tatcggattt tccggagtaa aagctttctc
    13081 cagcagtttg gtcagcttat tgacgattcc gactttcatt aatacggtgg atggtgtaga
    13141 gtcaaatgtg acagtagcag taatagctac aggaattgct tttgttcttg cattcgtagg
    13201 tacattgatt ttaggatttg acgaacaaac acaagacaat caattagaaa acaaacatgc
    13261 gaatgcagga gaaccgatta cttcagcacg gcatacctta aaaagtcctt taacgggtaa
    13321 agtactgcca ttgagtgaag taccagacca agttttttct tctggcgtga tgggaaaagg
    13381 tatcgctatt gatccagaag taggagaact agttgcacct gcagacggag agatcacgac
    13441 tatttttcca actggacatg ctgtagggat cacaacgact gacggagcgg agattttgat
    13501 tcatatcggg atggatacag tagaattaaa tgggaatggc tttgagatct tagtcaaaca
    13561 aggggatctc gtcaaagcag gagatttatt gattcgtttt gacatcgaag caattagagc
    13621 agctggctat agcgtaatca ctccagttgt cattacgaat actgatgcat ttgccgatat
    13681 acttgaatta gaccaaaaag agattattgc aaacgaagat gttctggcaa ttgtaaaata
    13741 aggaggaaat caggatgcaa tcacgttttt cagaaaaatt tttatggggt ggagcaactg
    13801 ctgccaaatc aattagaagg ggcttatgat cgagacggaa aaggactatc cgttgcagat
    13861 gctatgcctg gaggtaagca gcgctttgcg attatcggca gtgaagaatt tgactggacg
    13921 attgatcaag aaaaatatat ttacccgaat catcgaggaa tcgatcatta cgatcgtttc
    13981 aaagaagatc ttgccttgtt tgcggaaatg ggattcaaat gttatcgttt ttcgattgcc
    14041 tggacacgaa tttttccaaa tggagacgaa ggtactccta acgaagcggg attagaattt
    14101 tatgatcagc tgattgatga gtgcttaaaa tatgatattg aacctgtgat cacgatttct
    14161 cattacgaaa tgcctcttca tttggcaaaa gaatacggtg gctggaaaaa tcgaaaactg
    14221 atcgacttct atgaacgatt tgctcagaca gtattagaga gatacagcag taaagtcaaa
    14281 tattggatga ctttcaatga aatcaattcg gcctttcact ttccagcttt aagtcaagga
    14341 ttagtaaaaa gcaatggagc aggagagtac caaaatattt tccaagcatg gcataatcaa
    14401 ttcgttgcaa gtagcaaagc cgtaaaaatc ggccacgaat tgcgttcgga tatccaaatc
    14461 ggctgcatga ttatctatgc aacaacttac agcatcgatg ccaatccagt caatcaggca
    14521 gcaacaatga tccaaaacca agaattcaat tttttctgca cggatgtcca agtacgtgga
    14581 gaataccctg catacacagc gcgtacccat aaaaaatatg gagtcgatcc ggaaaaatta
    14641 gagcaaacgg aaaaagactt ccagttgcta aaagaatatc cagtggacta catcggattt
    14701 agttattata tgtctacggc aatcaatgag actgatccac aagcagcaac ttcagaaggg
    14761 aacttgctgg gaggagtaaa aaatccgttt cttgaggcaa gtgaatgggg ctggcagatc
    14821 gatccagaag ggctgagaat cgcattgaat gaattataca atcgttacca gaaaccatta
    14881 ttcattgttg aaaacggtct tggagccatt gatcatatgg aagaggatgg cacgattatt
    14941 gatgattatc ggatcgatta tttacgtcgt catatcgaag cgatggctaa tgcagtagca
    15001 gacggtgtag atttgatggg ctatacgcct tggggatgta tcgatcttgt cagtgcatca
    15061 acaggagaaa tgagtaaacg ttacggcttt atctatgtgg atctagacga tgaaggaaat
    15121 gggacactga atcgatcaaa gaaaaaatct tttgattggt ataaagaagt aattgcctca
    15181 aatggcgtaa acttgtaatt aaagcagctg aatgaaaaag aacacacttc tcacatgtga
    15241 tttgtgttct ttttttattt tttatgtgct attcttttag taatagtaaa gcaaagggtg
    15301 acgttatgaa aaggacacgt gtactatact tggaagtcgc agataaaatt aaagaagata
    15361 ttttctcagg aaaatatcca gtcggttcga tgttgccgac agaaagtgaa ttagaagaac
    15421 tgtttcaagt cagcaaaatc actgtccgaa aggcaatcga actgctggca tcagatgaat
    15481 atgtcgcaaa gaaaagtggt cgtgggacga cggttctaag caatcgtcct tacaatcgat
    15541 tatccaaagc ggcaagcttt acgcaaatct tggagaactc ccgatatgaa gtccggaaag
    15601 aaacgctgga tttgcaattg gtccatataa agaaggaaga tcctttgttt tcctttttcg
    15661 gtgagcaagc tgtttgtttt aagcgcttat attatttgaa caatcagcct tatatttatt
    15721 ttgttcatta tcttccacaa gaaatgatgc aattcagcag agaagaattt ggcaaagaat
    15781 ctttgtatcg tttgctgacg aaaaaaggat atactattga ctcattttct gatgatttct
    15841 cggcagtatt tttaacagaa gaagaacaag gaattctgaa aacagacgca cagatcggca
    15901 tcaaacggat acggaaatcg tctacgcaaa ttggggaaat gattgaatat tcggaggcgg
    15961 tctaccatac ggagctgcat ccttaccata ttgattatga aacataaata aaaaaagagg
    16021 ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcat
    16081 atttttcgtt tggttaggct tattaccgca gatacacggc tgtttctgga ttaccattta
    16141 ttctggataa gggggtgtgg cacgattttc agaaggaatc tatgaaccaa tcactgtatt
    16201 gattttagct tcgatggtaa ccccattcgg cttgacgatc gcttatttct tagggaaaat
    16261 cattcgtaaa aatatactca atcgtcaaga aatcgataca ttgaagaccg cttttcctat
    16321 ggggatctgt cagatcacag agggatgttt ccctatcgta ttgaacgact tggcacgcaa
    16381 tgtcatagca acgggagtcg gtgggacagt aggtggcgga ttaagcatgt tttggggcgc
    16441 agatagccgt attccggcat cgggaatgtt tgcagtcgct acgatgacca gaccatgggc
    16501 attcattggt gcattgttag ctggttcttt tgtcacagga ataaccattt tattattgaa
    16561 aaaaccagta gatccgaatg cagaaatcat ccaagaagaa aaagaggaag aagacatatc
    16621 ttgggatgat ttgacaatta gttaggagag gcagaatgga ttacaagaaa aaagtacaat
    16681 agatgtacct tgagacagga atggtatgaa agaatcagag ttagaaagaa tcgaatatac
    16741 agattttggt ttaggagcga tcgaaactgt ccagagcatc ggcaccctcc ttgaaatgac
    16801 gatgaaggaa aaatgagaac ctttcgttgt cgaaagggtc tggtatacct tatgtcgaag
    16861 gaaaggaaac aaaaaaactt tcctgtacta ttccaaaaag gaaagaaagt tattatacag
    16921 taaaaaaaga aattattctt catgctagag atcagtacac gttcgagccc aatatcaaac
    16981 actagttcca ggcaggcgca acaggtgcgg ttatcactga attttcttcc agcagtgacg
    17041 attcaagtga tatttttaca aatccagcta tttatcgtta acaaaaagat ttttaggaga
    17101 agctgatgaa caaaaatgat caaattttga tcgtttagtt cgtcggcttt tttatttaga
    17161 gaaacatcat ttatcagtga aacataacac gagaacaaaa tgattttttc gttaataaaa
    17221 gaaacaagta tttgttgttt aaaataatac acgttattgg catataggac gaaatcccag
    17281 tatcttctat aagtaagtca tttcatatcg atgaaaacac gttatttatt tttgttttat
    17341 ctataaaatg gctgaaaaat gatttttttc t
//
LOCUS       DACCXO010000053        17206 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.054, whole genome shotgun sequence.
ACCESSION   DACCXO010000053 DACCXO010000000
VERSION     DACCXO010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.054"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..839
                     /locus_tag="IVY70_001618"
     CDS             <1..839
                     /locus_tag="IVY70_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7958749.1"
                     /translation="YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGV
                     VLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
     gene            complement(1263..1433)
                     /locus_tag="IVY70_001619"
     CDS             complement(1263..1433)
                     /locus_tag="IVY70_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7958750.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1627..1806)
                     /locus_tag="IVY70_001620"
     CDS             complement(1627..1806)
                     /locus_tag="IVY70_001620"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958751.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            complement(1816..1992)
                     /locus_tag="IVY70_001621"
     CDS             complement(1816..1992)
                     /locus_tag="IVY70_001621"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958752.1"
                     /translation="MITEVSYETLQKKKTLIVAAVLFPAPCVCRNCGSAVVDGNRKLM
                     VVKNKKKETIVHFE"
     gene            2193..3305
                     /locus_tag="IVY70_001622"
     CDS             2193..3305
                     /locus_tag="IVY70_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7958753.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3383..3676)
                     /locus_tag="IVY70_001623"
     CDS             complement(3383..3676)
                     /locus_tag="IVY70_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP7958754.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3844..4158
                     /locus_tag="IVY70_001624"
     CDS             3844..4158
                     /locus_tag="IVY70_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP7958755.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4179..6158
                     /locus_tag="IVY70_001625"
     CDS             4179..6158
                     /locus_tag="IVY70_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7958756.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6171..6497
                     /locus_tag="IVY70_001626"
     CDS             6171..6497
                     /locus_tag="IVY70_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP7958757.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6513..7031
                     /locus_tag="IVY70_001627"
     CDS             6513..7031
                     /locus_tag="IVY70_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958758.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7031..8383
                     /gene="celB"
                     /locus_tag="IVY70_001628"
     CDS             7031..8383
                     /gene="celB"
                     /locus_tag="IVY70_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP7958759.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8467..9972)
                     /locus_tag="IVY70_001629"
     CDS             complement(8467..9972)
                     /locus_tag="IVY70_001629"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP7958760.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10064..10654)
                     /locus_tag="IVY70_001630"
     CDS             complement(10064..10654)
                     /locus_tag="IVY70_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7958761.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10833..12479
                     /locus_tag="IVY70_001631"
     CDS             10833..12479
                     /locus_tag="IVY70_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7958762.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12528..13247
                     /locus_tag="IVY70_001632"
     CDS             12528..13247
                     /locus_tag="IVY70_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP7958763.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13726..14673
                     /locus_tag="IVY70_001633"
     CDS             13726..14673
                     /locus_tag="IVY70_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7958764.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14685..15605
                     /locus_tag="IVY70_001634"
     CDS             14685..15605
                     /locus_tag="IVY70_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7958765.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15646..17103
                     /locus_tag="IVY70_001635"
     CDS             15646..17103
                     /locus_tag="IVY70_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7958766.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
BASE COUNT         5402 a         2958 c         3510 g         5336 t
ORIGIN      
        1 catatcagct ggtagaaact caagcgccaa cggggtatga attagatgcg acaccgatcg
       61 agtttgagat cgaaagaagt cagacagcgg tggtagaatt gacgaaagca aaccgactta
      121 caccaggcgg tgtcgtgttg acaaagatcg atgaccaaag cggggaagtc ctgcaaggag
      181 ctgtttttga gttacaagat gccaatggaa ccgttctgca gtcaggtcta accacaggag
      241 cagatggaaa actggcgatt gacggcttag caccaggagt gtatcagctg gtagaaaccc
      301 aagcgccaac ggggtatgaa ttggatgcga caccaatcga gtttgagatc gaaagaagtc
      361 agacagcggt ggtagaattg acgaaagaaa atcgacttac accaggcggt gtcgtgttga
      421 caaagatcga tgaccaaagc ggagaagtcc tgcaaggagc tgtttttgag ttacaagatg
      481 ccaatggaac cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgatag
      541 atggcttagc accaggagca tatcagctgg tagaaactca agcgccaacg gggtatgaat
      601 tggatgcgac accagtaact ttcaagatcg aaaaagaaca agaagtggcg atctttttaa
      661 caaaagagaa tagaaaagtt gctgatagct cagatataag aggaaacaat ccaacaagca
      721 gtggaaataa gcatcttcca aaaactggag aaaccatcat tagccagttt atattgtcaa
      781 tattgggtgt gctacttgtt ttcatcagta taaaatttag aaagaaacga aatatatgag
      841 ctaaaataaa aaattgaata agcatagttt ttaaaatcaa ctagtaccca tcaattgaat
      901 ggaaactacc gattttagaa ggggtataca aacaaatatt agtatcggag tagagaaggt
      961 tgaacccaac cagtaaataa gtaaagacaa ttttgtgctg cgttgagaag tgaaaaataa
     1021 acctttgtag taatactagt taataaaaaa tatacaaaaa cactgcgaaa atcattcttg
     1081 agtttcgcag tgttttttgc ctaatgactc aagtttattg ggtaatactt tgattttctt
     1141 tctacaaata atcagcgatc agttgtaatt ttttctctag ttttaatggc tctttgtcaa
     1201 taaggagaaa aacaagtcgg gtcttttgtc tcggaaagct tcaaccaact cgtgaataaa
     1261 ggttacgcct ccttgaaagg cgtggaaaaa ccaagtaatc gatcaatcac caatttcgca
     1321 tttggtagaa catgtttggt gagctgaaag taggtggcgt tcatgtctgt aactaagaat
     1381 tctatttctt taggattagt gcagctaagg aaatagtttg ttaatcgagg aattttttgt
     1441 gtaggtaaat tttctcgatt aatttttctg tctcgccatc cgcggaaata aaactcagtt
     1501 tatcctcaac ggaaacgtga gaacgaaatt cacaaccctc aacactcaag acagaattct
     1561 tttggaagac tttggtaaat agcttctcaa ctctttcaat gtgcgaataa cggttgtcaa
     1621 agatacctaa caacttttcg aaataaaaaa taacgacact ttttcagtca gtaaagaagt
     1681 gattttatgt ctgacatgat ttgaaatcga gtgccttgga cggataaaat aactttgagc
     1741 gttccaatgg cttcgacagt ttttacaggt atagcgctgc ttttttaggc gtataaccat
     1801 tggcatatga ttatattatt caaagtggac aatggtttcc ttcttcttgt ttttcaccac
     1861 cattaatttc ctatttccat ctaccacagc agaaccacaa tttctacaga cacatggagc
     1921 agggaagaga actgcagcaa cgatcaatgt ttttttcttc tgtaaggttt cgtaagagac
     1981 ctctgtaatc atcaaatttt tgtctattga tctcagaatt tttttgatag aaccattcat
     2041 aaagcatatc gttctctcag ttgtttattt tgtggtgatt taatcataca agaaaacgat
     2101 atgttttttt ataaaaacat gaataggaaa aatagttgac aagaaaatga caacgccata
     2161 tgatgttcca tagtatttac aacctgtcat agatgactgt aaaaaataaa agtaaaaaga
     2221 aaggacgaca acaggtggat ttttatcagt ctctacaact agatccgttt attttaaaac
     2281 aaaaaatacg agaagcagct tctaaaaaag aaaagtgcat gtatatttgt tcattgtttt
     2341 tacgatcatt attcatcgta ttgtttgcca tttgttttat cattataatt acaacattat
     2401 ttgaatcgaa gcataaacca tacgctgtgg ttttattctg tatgttgatg agtattcgtt
     2461 ttgttgactt tggttataag atttcccatt cgatcatagg tttagcaatc gtcatgttct
     2521 cactattagt agcaccgtat gtccagctga tcaaatggtc agttatgggc atgttgattc
     2581 attttatttt actaagcagc atattaatgg caaccgctag tgatcccaaa atggggaatg
     2641 cttctttgta cggcttttca tatctcttta ttgtttattc gttacctaaa gattcgttga
     2701 acaagaattt ctttacacaa acaagtagct tattattttt gtttttttgt tggttttcag
     2761 ttctccttta cagaaaacat cgagaaaaga acaaagacaa aagcttgttt aaagagattt
     2821 ttttgaaagg catgtattcg caggaaaaaa tttggatgct catctatgca tttggcatta
     2881 gtcttttgat cgttgcagga gagtatgtcc cttttcagcg attgatgtgg gcagggtttg
     2941 cattttcttc tattgtatct agctatggat tgatgtcgat tggtttcaaa gaacgagcag
     3001 tagaccgaat cattggctca ttgattggat gtgttttatt tattggtatc agccaattta
     3061 ttccttttaa ctgggtcggt atattaggtg gtctagcttt aggtgtttgt tctacctata
     3121 gatataaaac agtttttaat tcttttggtg ctttggcaat cactgccagt ttattcgggg
     3181 ttccaggagc agtaacgatc cgcatttttg agaatatcct tggggtatgt ttaggaatca
     3241 tgtatattgg ggtgactgaa atacttatcc gaaaaataag agaaaaacac ggtttgaatc
     3301 attagatgag atcgaataaa aaataaggaa aaagtgaaga tacacatgga tgataaattg
     3361 ctatcattcc atgtgtatct ttttacggta aatcagtgaa acgttcgact ttcattttta
     3421 atttatactt tttacggagt tcgtttattt gagccatcat ttcggatttg tgatgctttt
     3481 cgattgcttc ttcatctttc caacggtcga tcagtaaaag agattctgga tcttctagtg
     3541 gaaagtaata gtcatatttc tcgtttcctt cttctgcacg aatcgctttg acaatgtttc
     3601 tttcgaccat ttcttctgca aattttctgg cttgtccatt tgtacttgta tacaaaatat
     3661 tcactgtgat actcactaaa tcccctcctg aaaaaattct atcactagta tatcacggtg
     3721 ccagaaatta ttattcaaga tatgatacac attttttccg caatgctacg gaaatatata
     3781 ttttgaatag aaaacgctta caagatatgc ttggtttata aaaataaaag gagtgattga
     3841 tttatgaaaa aagcattgat tatttgtgcg gcaggaatgt catcctcgat gatggcctca
     3901 aagacaacgg atttctttaa gcaaaaaaga gaagaggtac tcgtagatgc agtttcagct
     3961 acagaaggcg ataatatgat taagaatagt gattttgatt tatttctgat cagcccgcaa
     4021 acgacgatgt ttttggataa attcaaaaaa ttaggagaat ctgttggaaa gcctgttgtt
     4081 agtatccctt tccaagcgta tgtcccaatc cctacaggca tccagaagtt agcagaggtg
     4141 atcgaagaga acatatgatg gaataggagt ggatgataat gaaacagaag gaaaaattgc
     4201 tgttgagata tttgattgat cacaagaagg agtatgtcac cagtcaacgt ttagcttctg
     4261 aattgttatt atctgatcgg acaatccgta attacttgca gcggatcaaa gagttagtag
     4321 aaaaaaacgg aggtaagatc attgctaaac caggttatgg ctaccaactg catattttac
     4381 aaagactgac atttgacttg tttttgagtc ggcaagaaat agtacctgga tatgctagag
     4441 aaccgcaagg attttatgag tccgaagatc gacaaaagta tatattgaac caattgcttt
     4501 tggaagatgc cattcttttt atggatgatt tggcagaaga gctttatatc agtcgttcca
     4561 gtttgacaaa ggacatgcaa gaaatcaagg agcgattgat tccatattca ctaaaaatcg
     4621 tttctaagca tggtcaaggg acatggatcg aaggggaaga aagaaatcga cggcatttta
     4681 tcatggatac tttttttgga aaccattatg gaaattcatt gaaagaatat ttagggcaaa
     4741 gccgtttttt cccagacatc agttttgaag agctagtcat tatcattctc gatgaaacaa
     4801 gagaagctaa gttaaaagtt tcggatttta tcatacagaa tctgacttta cacttggctt
     4861 tggggatcaa acggctccga gaagggtttg aaatcaagga attaggcatc gaacaagaag
     4921 tttctaagcg agtagagtat caagtggcgc agcggatcgt tcaacgtatt gaggtaatcg
     4981 caggtgtatc gtttcccgaa gaagaaattg cttatttgac attacatctc atggctaagt
     5041 cgaatcacaa agaaaatcaa aatgatcagg aactatcaaa ggaattagcg aaagttctga
     5101 aaagattgtc tcaggaattt catttttcat tagtagagga tctccagctt ttcaatggac
     5161 tactcgatca tttgtcaccg atgatgattc gattgagccg aggtattcgc attgagaacc
     5221 cattgactga ggagataaag aaagaaaatc ccaaagtctt tgacgcagta aaaaggcatt
     5281 tcagcaacat gccggctttg aaaaactata ccatcaatga agatgaatgg gcctatttgg
     5341 ctttgcatct catggcggca ttggaaaaag aaagagctgc gcataaacta catgcattga
     5401 ttatctgtgc aacgggttac ggcagtgccc aactattaaa gaatcgcgta gtcagtgaat
     5461 tcggtaaaaa tattacagtg gtaagtgtaa aaggctatta tgagatcaat gaaacaactt
     5521 taaaaggaat cgacctgatt atttcttcta ttgatctttc gacaatgttt ttcaaaattc
     5581 cagttttaca tgtcagtatt tttttgaatg aagatgatgt tcgtaaaata cgaaaggtag
     5641 ttggagaatc tataccctat cattcctcag atagaccgat accagcattt tctctacagc
     5701 aaaaaaagca gatttacacc gaacagttgt ctgaaaaatt tttcaagact tatacgtacg
     5761 aaccgaaaaa ggaagaaatt cttcatgatt tgctgcaaaa gctgagcata aatgaagaag
     5821 aaggatatat aagagaaatg cgaaagcaga tccagcaacg tgaaaatatg ggacaaatca
     5881 tattcagtga tacagtggca gtcccacatc ccgctgttcc agttggaatc gaaacaaagg
     5941 tagccgttgc attgattcct tctgggatgc agtgggatca atatcaagaa attcattttg
     6001 tttttttagt ttcgccttct tatgtcgaaa atgaaggaat tacggtcgtg actaaagcaa
     6061 tcgttaggtt agttgaccag ctaaaagtac agcaggaaat cttagctgaa cctatttttg
     6121 agaatttcag cagtaaattt atcaaactga tcgaataagg aggaaaacag atggctgaaa
     6181 aaataaacag tgaaacacta caagtgactt cttttgatat cattttccat agcggaaatg
     6241 cacggacact gatacatgaa gcatttcgct cgatgcgtaa agagatgttt gaagaagtag
     6301 aagagaagtt gcgacaagca aatgaagaga ttcttcatgc gcatcagtca caaactcaac
     6361 ttctgaaaga atatgccagc gggacaaaaa tagaaatgga aattatcatg gtccatgcac
     6421 aagatcatct gatgacgacg atgacattat tggaagttgc acaggaaatg aattatttgt
     6481 atcaaaaaaa taaatagaaa ggaaggatca ttgtgcagga tacagtaaaa aggaaaatcg
     6541 aattagaaaa aaagcaactt agtttgaaaa acatgtattt caatcgttat ctaatcgtac
     6601 gttacctgac tgcctttttc tttttcatga acatgcaatg gatgattctg cttctatcag
     6661 ctaaaagttt aggaagtagt ttaccgatga ttctgatttt agctattttg cctgcagtag
     6721 gagaacaagt aaagctttat cgaaaacacc aaacgaacgt tccatggaca aagtggtatt
     6781 ttttgttcca gagtgtcttt aatttattag tgattccagt gttgtttacc tcaggattta
     6841 cattcttgta tccttttatg gcaaataaca atggaggaca gttatttgtt ttcattcttg
     6901 ttgtaagcgg tatttttgcc agtgcattta tacagtatcg tttaaagaaa atttcactca
     6961 accaagacca acagtatatc cgtattaaac aatatgaaaa agcgttatat ttaggaaagg
     7021 agaataacta atgtttgatt ttttgcagaa atacctgatg ggacctatgg ggaaaattgc
     7081 gcaacttaaa atcgttcggg ctgtcatggc agcggggatg gcttctattc ctttcacgat
     7141 tgtaggctcg atgtttttgg ttttaaatat tttgccatta ccgttcccat ttttagaagg
     7201 attttttaat gcaacttttt tcaaagttag tgacttgtac atgatcgtca atacaatgac
     7261 gatggggatt ttatctattt actttgccat tgtgtttgct tatgaattaa cgtcaattga
     7321 acgagatgaa caaaaattaa atgttaatcc gctcacgggg gcattactat ctgtttttgc
     7381 attctttatg tgtatccctg aattgatcat gactgatggt aaaatcagct tgattgcttc
     7441 tatcaatgag acagaaactg tagtaaatgg tattaggatg ggctcttttg tcgaacgttt
     7501 aggaacttca ggaatcttta cagctattat tatgtcgatt attgcggtcg aattgtattg
     7561 tatgtgtgtc aagagaaatt gggtcataaa aatgccagat gttgttccgc caggagtttc
     7621 tcgttctttc actgctctga tcccaacatt tgtcattgcg tttgtggtga tgatcatcaa
     7681 tggattatta gttgcactag gaacggatat cttcaaaatg attgctatcc catttgggtt
     7741 tgttacgcag ttgacgaata catggcttgg tgtcatggtg atttatttct tgatccatgc
     7801 attgtggatc gtagggatcc atggagcgaa tatcatcact tctttcttga ccccgatcgt
     7861 attagctaat atggcagcta acgcacaagg cgcaaattat ccgttggcag gagaattcaa
     7921 caattcttat gtaaccgttg gtggatcagg tgcgacttta ggattgatca tctttattgc
     7981 attcctcgcg aaatctgatc agctgaaagt gttaggaaaa gcctcacttg taccaggatt
     8041 attcaatatc aatgaaccaa tcattttcgg gatgccaatc gtctacaatc cctatttagc
     8101 ggttccgttt ttcttagctc cgatggcttc tgctacactc gcttattttg ccattcggct
     8161 ggaaatcgtc aaaccgatgc ttgcacagat gccgtggcct tcaccagtcg ggatcggagc
     8221 atttgttggt agtggtggtg attggaaagc tgctgtgtta gcagtcattt gtgcaatcat
     8281 cgcattcatt atctggttcc catttatcaa attctatgat aataaattat taaatgaaga
     8341 aagagaaaag gcagcagaat tagctagtga aaagttaaaa tgatttctaa gaaaaaagga
     8401 actgtgacaa aaaatcacag tccctttttt tcttagaaat gggaaaccac tagtatataa
     8461 ataccattat tcttcaatca accgcttcac gattgcataa tccccttcat aaggttcatc
     8521 tacaccattg attttttgat agggatccac gccttttccc gcaataacga ctgaatcttg
     8581 cggagcgctt tctgctaaag cgcgtcgaat cgcttcagga cgatccattt cataatgaac
     8641 gatcaaatct ggattagtaa ttgcttcatt gatttcttcc gcgatttttt tcggatcttc
     8701 aaatgctggg tcatctgccg tcaaaaaggc gatgtcagct gtttctgata gcacttttcc
     8761 aaaatcatgt cgacgggaga ttgctttgtt tcctgggctg cctaatacaa caattacacg
     8821 gccatctgga tgttcatttt tggcaaattt cagcaatgtt ttcaagctta gataattatg
     8881 tgcatagtcc acatagacat gcgctccatt ggattggatc aattgatcca ttcggcctgg
     8941 tacacgagct tcttttaatc cgtcttgtgc atcttgactg gaggcacctg ttagtgctgc
     9001 ggcaatgatc gcactggctg cattttcttg gttgaatcct ccagccagca aaatatcata
     9061 agcagtatcg cagatagcta agcgatcttc atgagaagat aaagtaaaac cgtgagtacc
     9121 tttatctgaa cggatcactt gataattgct ttcttccgaa cgaccataag tgatcgttgg
     9181 aatctggaat agatcggctg tttccctaag taaatcaaaa tagtcacttt catgatttaa
     9241 aacaactgtt tttgaattca agatcaattg tcgcttgcaa tagaaatagt catcaaatgt
     9301 cggatgttcg atcggactga tatggtcagg cgagatattc aagaagattc cgacatctaa
     9361 agtcaagccg tatacgcgtt gtgttttgta agcttgtgaa gaaacttcca tcactagatg
     9421 agtcatacca ttttcaactg cttctgacat catacgatat aaatctaatg actcaggcgt
     9481 tgtcaaatgg gatttgaaat atgttttgcc atctaacgtt gtattcatag tagaaagcaa
     9541 tgctgttttt ttatttgttg tgtgatccaa gattgctttt gtaaagtacg cagcagttgt
     9601 tttacctttt gttccagtga atccgatcac tttcaattta ttttgcggat agtcgtaaaa
     9661 agccatagca agaagcgcca ttgctttacg gatatccgtt acgatgattc ctactgctga
     9721 attttcgtat ggttgttctg atacataata ttgaataccc tgttcgattg ctgaatccaa
     9781 atattcttct ttgaaattca ttcctttaca gaaaaacaaa gtagaagcat cagcttttct
     9841 tgaatcataa cttaactgaa gaaattcagc tttttctggt acatcaagat gccagcctct
     9901 tgttgaaaca aattctttta atagattttc ttttaatagt aaatgacgga tgtcttctaa
     9961 agagaagctc attgattgat ccctcgcttt attcaatttc ttggtttatt atagcacact
    10021 ctttttaata tatcgtttgt ttccgctatg acaaaatcca gctttattca aattttcttg
    10081 acacagggga caagcggatg agtattttca taaacaatga actgactgta atagatagga
    10141 gaacgaatag tgtctccaac aatccaaaaa cataataatt caaaaagacg ataagactat
    10201 tggtaagtaa aagtccttgc ggcattggaa gccgaaaaaa tttgtggaaa ataatcggcg
    10261 gtatcgttat accgccattt gaggccccga ctgtgtacaa tgtgtaaacc ccaagactga
    10321 acaaaagact tcctgaaatc agtgaaaaga aatgtgagga gatcatttcc acttttggaa
    10381 caatctctaa aacaacagga aaaagcagac ttcccgctac agtcttaaaa aatactgatt
    10441 ttcctaatag gacagcggct agaagcagca tcataagatt gatgctccaa acagtaactg
    10501 agcgttggat agaaaaggcc gtttcaacta aatgtccgat ggcgcccact ccggcggaag
    10561 ctatatcgtg agggagcaag aaccaattca atccaactgt gataaagaaa agacctaaaa
    10621 atataacggg tagttttttt atttcttgtg tcattgatat ctcctataaa tgttagccat
    10681 aaaaggctgt atatttcttt actttaccaa aaaaacgaaa ccgaattcaa aaaaacaatt
    10741 gttcttataa aagttatata aaatcgattg ccagtttctg ttttaccaat taaaatgata
    10801 gtaatgtttt gtagaaagtt ggaatctgac cgatggataa tcaaagaaaa ccagcattag
    10861 atcaattaac accgcaagaa aaaatggcac gaggctctgc ttggatgacc gccagtaata
    10921 tcatttcaag attgctaggc gctgtctata tcattccctg gtatgcttgg atgggcgaaa
    10981 atgctaaggc tgccaatgga ttattcaata tgggatacaa tatatatgca ttgtttctac
    11041 tgatctctac agcaggaata cctgctgcga ttgcaaaaca aactgcacgc tataattcat
    11101 tgaatgaata tggcacttca cgacgattat ttatccgtgc attacagatg atgggcgggt
    11161 taggcctgtt gtttgctttg ttcatgtaca tcgcctctcc ttggctagct catgcttctg
    11221 gtggcggaga agaactgatt ccaacgatgc gttctttgag tatcgctgta ttggtttttc
    11281 cttgtatgag tgtgatacga gggtattttc aaggcaatca agaaatgatg ccttatgcgc
    11341 tgtcgcagat tgtagaacag gtggcacgta ttttttatat gctattggct acattcatca
    11401 tcatgaaagt catggatggt gactatgtga cggcggtcac gcaatcaact ttcgcggcat
    11461 ttatcggtat gctagcaagt attcttgtcc ttttgtattt tttgaagaaa catcaagcgt
    11521 atacatcggc gtttattcac tacagcgaaa acaaaataaa tattgcaacg aaggaattgt
    11581 tgttagatac cgttaaagaa gcgattccgt ttattattgt gggttcaggg gtaacgatct
    11641 tcaaattagt cgatcagttc acttttatga agatcatgtc tgcatccacc gagtattcca
    11701 atgcacagtt actggatctt ttttcgattt ttagtgccaa tccagataag ttgacgatgg
    11761 tagttattgc attggcaaca tcgatcgcgt ctactgggtt accgttgatt acagaagctg
    11821 ttacgttgaa agatcgagta ggtctggcaa aattgacaag cggaaattta cagttgtttt
    11881 cattttttat gtttccggcg acatttggtg ttatgttact tgcctatccg ctaaatacat
    11941 tattttatac accggattct ctaggaagta atgtactgat ccaagcaagt tttgtaggac
    12001 tcttcctagg actatatatg ctagtttcca acatgctgca aggaatgttt gaaaacaagg
    12061 cggcaattaa ttatttggtt attgggtttc tagtgaaact ccttcttcaa tacccagcca
    12121 ttcgtttatt tgaagtatac ggccctttat tggcgacaat gatcggattt gctgtgtctt
    12181 gtaccttgat cttaaagaga atccatgaag tagcccgctt taaaccaagt tttgtctggc
    12241 gcaggacatt gctgatcttt attcttacct tgattatgct tttggcttcg tggatcacaa
    12301 agatgatttt tggtgctttt ttaaacgaag atagcaaatt ccagtctcta ttgttgattc
    12361 ttgtagtagc aggtataggg gctttagtgt atggttattt agctttgaaa atccgcttgg
    12421 cagaaagact actaggacca ggaatagcgc gtttacgaca taaattaaaa ataaaataag
    12481 tgaatcagaa gagctagatt gatgctcttt tgaaatgggg gaaacgcatg cgtttagata
    12541 agtttttagc agatatggga cttggcagcc gcaaagaagt caaaggatta atcaaaaaag
    12601 gaacgatcga agtcaatgga caagtggtga aaagcgataa agcgcaagta aatgaattcg
    12661 aagatgcggt tacttattta ggagaaatca ttacgtatca aaaagatttt tactacattt
    12721 tgcataaacc tgcaggagtc atttctgcta ctcaggataa tcatgatcaa accgtaatcg
    12781 atttattgga agaccaggat tatcgggaag atctttttcc agtcggccga ttagataaag
    12841 acacagaggg cctcttgatt ttaacgaatg acggggtgtt tgcccatcag ttactatcac
    12901 ctaaaaaaca tgtggagaaa gaatatctag ctgaggttag cggtataatg acagaagaag
    12961 atatccgttt atttgcagaa ggactgatta ttgatggcga tgagcagaca ttgccggcaa
    13021 aactccaaat tgatcaagta gatcaagaga cagaaacttc aattatccgt ctgattttgc
    13081 atgaaggaaa gtttcaccaa gttaagcgaa tggtcaaagc agtaggaaaa gaagtcttat
    13141 atttaaaaag aatccgtatg ggaaaatttt ggctgcctaa agatttgaat aaagggcaat
    13201 atcgacccat gaccaaagaa gagctagaac aagtgagaga aaaataattg aatggatagc
    13261 taaaagaata agcagagtag ttgtagcgcc atctgaaaag cagacttgtc cgtctgactt
    13321 tcggatggct tttttgtgaa atttcggctt atttccgtag aagctacttc tggaacaccg
    13381 tttgttcgga ataagggact gtgacaagac ttttgttaca gtccccattg ttccttgtga
    13441 ctcagagtta gatagtaagg acacgatctc cctaatactc aggccaaaac gctgaattca
    13501 caacctctga taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaacggcaa
    13561 aatatgagga actattttcc cgtttttttc tctaatactc aggccaaaac gctgatttca
    13621 caacctctcc tatactttgt ttcattttcc ctatactttg tttatttatt ctcattattg
    13681 aaagcgctat aattacgttg cgaatgaatc gaaaggggtt tttagatgaa aaaacgaaag
    13741 gggttcaatc atttttttat gaatctatgg cgttataaag cacttgtcct gatggctgta
    13801 ccgggtatgc tttggatgat ttttttcttt tacatacctg taatggcgaa tgtcgtagca
    13861 ttcaaaaatt ttcatatttc agcagatggg ttcttggcta gcttgaggga aagtccttgg
    13921 gtgggttggg aaaatttcaa atttttattt tcttcggatc aagcatttct catcactaaa
    13981 aatacaattt tatataatgt agcatttatc cttttgaatc tgctgatctc agtcgtattt
    14041 gcaatcatta tgagcgagtt gagaaataaa cgactcgtta aagtttatca aacgatgtcc
    14101 ttactacctt attttctatc ttgggtcatt atcagttact ttgtttacgc ttttttaagt
    14161 cctgacaaag gaatattcaa tcagtggatc actggacatg gaggagaagc tgtcaattgg
    14221 tataatgaac cgaaatattg gccgtttatc ttggtgttca ttggtacatg gaaagggatt
    14281 ggttataaca gcattattta ctttgcatct gttatgggaa tcgatccgac ttattatgaa
    14341 gctgctatgg ttgatggagc tagcaaatgg cagcagatca aacatgtgac gataccacaa
    14401 cttattccac taatgacgat tttaacgatt ttagccgtag gaaacatttt ccgagctgat
    14461 tttggcctgt tttataacat tcctaggaat tccggtgcat tatatgatgt gactcaagtt
    14521 ttggatacgt atatctacaa tggcctgaca tccacaggag attttggtat gacggctgct
    14581 gcaggacttt accaatcagt tgtcggcttt gtcttgttga tgatcacaaa tacgatcgcc
    14641 cgacgttttg atagcgattc tgcattattc taaggaggaa aaaaatgaaa aaaaagaaag
    14701 tggagagagt ccaagtccgc tctttcaata agacggctaa ttttttctct aatataatga
    14761 ttgcactctt tgctatttcc tgtattttgc catttatatt tgttatcgcc atctctttta
    14821 ccgatgagtc catattgtcg actcacgggt acagtttttg gccagaaaag ttcagtatat
    14881 ttggttatac gtacttgttc gaccagatgc aggataaaat tttccaagcg cttttcgtca
    14941 ctgtacttgt tactgtttta ggtacactga tcaatagcac ggctacttct ttatatgctt
    15001 atgcgatttc acggtcaaac tttccattcc gtcgtttttt taccgttttt tgtttgatta
    15061 cgatgttgtt ctcaccaggg atggtggcca attatcttgt tatgacaaat cttcttcagt
    15121 taaaagatac gatttgggca ttgatcttgc caatggctgt cagtcccttc aatattattg
    15181 tgatgcgcac attctttaaa cgttcggtct ctgattcgat tattgaatcg gcacggatcg
    15241 atggagctag cgaactgcga atatttgttc agatcgtttt accgttagct gttccaggta
    15301 ttgcgacaat cagtttattt gccgcattgg gttactggaa tgactggttc aatgctttac
    15361 tatacatcca agatgataag ctagttccac tacagtatct tttgatgaaa atccaaagta
    15421 atattcaata tctgacacaa aattcaggag caggaagcca attgacaggc ggattagcct
    15481 cagttccagg agaatctgcc agaatggcga tcgtagtcat ctcgacgttg cctattgcga
    15541 tcagctatcc atttttccag aagcattttg tcaaaggtct aacaatcggc ggagtaaaag
    15601 aataaagaaa caaaaatata aatcataaaa ttaggaggaa gaaaaatgaa gttatggaag
    15661 aaactagcct ttacaggtgt ttcagcgttg ttgttgggaa cattagcagc atgcggaggt
    15721 tctggttcta aagaccaggc agaagctagc aattcggaca ctttgcagat gtatcaaatc
    15781 ggagataaac cagataattt cgatcaattg atggaagtag caaacaaacg aatcgaaaaa
    15841 gagatcggtg taaaagtcaa tatcaattac attggctggg gcgattacga aaagaaaatg
    15901 aacgtcatca tttcttccgg ggaaaattat gatatcgcct ttgcgaacaa ctatgtttcc
    15961 aatgcccaaa aaggagcctt tgctgatttg actgaattag cgcctaagta cgctgaaaaa
    16021 gcttataatg atttagatga agcatatatt aaaggaaatc tggtcaatgg aaaattatat
    16081 gctttcccag tcaatggaaa tgtctttgct caacaagtac ttacttttaa caaagcttta
    16141 ctagataaat acgatctttc aattgatgga atccaatctt acgctgatgc agaaaaagtc
    16201 ttgaaggaat tccatgaaaa agaaccaaac acggcagctt ttgccattgg acaaggcttc
    16261 aaagtccaag ggaactttga ttatccttta gggaatacgt taccatttgc tattgatctg
    16321 gatggagatg attcgaaaat catcaaccaa tacgataatg aaacgtatat cggactgtta
    16381 cgtacaatgc atagctggta caaacaagga ctcattccaa gtgacgctgc aacgagcaat
    16441 aaggattatc cactagaagg aaatacttgg ctaatgcgtg gagaaacaca aggaccgtat
    16501 gattatggcg atacaatttt gacaaatgca gcaaaacaag aacttgtctc aaaagcaatt
    16561 actgttccac taaaatctac agctcaagca caaatggcaa attttgtcgt atcaaatact
    16621 tcaaaaaata aagaaaaatc agtagaactt ctagggctgc ttaacagtga tccagaatta
    16681 ttgaatgggc ttgtttgggg gatcgaggga gaagcatggg aaaaagtcga tggttcagat
    16741 gataaaatca aattgctaga tggttatcag ccaaatacac atatgtctgc ttggaataca
    16801 ggaaacaaca agattctata tacacaagag tctattacgg atgacatgat tgccaaacgt
    16861 gatcaatcga ttgctgatgc agaaacttca ccgattttag gattcagctt caacactgac
    16921 agtgtcaaaa cagaattaag taatatttct aatgtcatga atcaatattt agatggattg
    16981 aatacaggga ctgttgatcc agatgaaaca ttaccgaaat tgaaagacgc tttggacaag
    17041 gctggctatg ataaggtatt gaaagaaatg caaaaacaat atgatgagtt ccgacaagaa
    17101 taataatgaa taaagacaaa ggggttgtga caaaagacgt gtcacaacct cttgttccga
    17161 ataaacggtg ttcaaaagcc agtttctcgg tattaagcta aatata
//
LOCUS       DACCXO010000054        16980 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.055, whole genome shotgun sequence.
ACCESSION   DACCXO010000054 DACCXO010000000
VERSION     DACCXO010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.055"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(449..1174)
                     /locus_tag="IVY70_001636"
     CDS             complement(449..1174)
                     /locus_tag="IVY70_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958767.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1328..1654)
                     /locus_tag="IVY70_001637"
     CDS             complement(1328..1654)
                     /locus_tag="IVY70_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958768.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(1691..2056)
                     /locus_tag="IVY70_001638"
     CDS             complement(1691..2056)
                     /locus_tag="IVY70_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP7958769.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(2093..2308)
                     /locus_tag="IVY70_001639"
     CDS             complement(2093..2308)
                     /locus_tag="IVY70_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7958770.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            2437..2772
                     /locus_tag="IVY70_001640"
     CDS             2437..2772
                     /locus_tag="IVY70_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7958771.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(2848..4845)
                     /locus_tag="IVY70_001641"
     CDS             complement(2848..4845)
                     /locus_tag="IVY70_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958772.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(4943..5218)
                     /locus_tag="IVY70_001642"
     CDS             complement(4943..5218)
                     /locus_tag="IVY70_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7958773.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            5450..6184
                     /locus_tag="IVY70_001643"
     CDS             5450..6184
                     /locus_tag="IVY70_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7958774.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQVPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            6223..7365
                     /locus_tag="IVY70_001644"
     CDS             6223..7365
                     /locus_tag="IVY70_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7958775.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(7993..8478)
                     /locus_tag="IVY70_001645"
     CDS             complement(7993..8478)
                     /locus_tag="IVY70_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HAP7958776.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(8480..9244)
                     /locus_tag="IVY70_001646"
                     /pseudo
     CDS             complement(8480..9244)
                     /locus_tag="IVY70_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9414..10607)
                     /locus_tag="IVY70_001647"
     CDS             complement(9414..10607)
                     /locus_tag="IVY70_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7958777.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(10691..10894)
                     /locus_tag="IVY70_001648"
     CDS             complement(10691..10894)
                     /locus_tag="IVY70_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP7958778.1"
                     /translation="MMNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVI
                     MNGNRIQIKRKQFEKIIDTLDTI"
     gene            11151..11393
                     /locus_tag="IVY70_001649"
     CDS             11151..11393
                     /locus_tag="IVY70_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958779.1"
                     /translation="MAHQPSGSAQTECVRMPYDAPPLSCGHIYQGAIRPPGKHRNGER
                     VSRFMLPGPVRQFVTIAWMSLISRRRFMASSRHTCG"
     gene            complement(11315..11548)
                     /locus_tag="IVY70_001650"
     CDS             complement(11315..11548)
                     /locus_tag="IVY70_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7958780.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(11555..11986)
                     /locus_tag="IVY70_001651"
     CDS             complement(11555..11986)
                     /locus_tag="IVY70_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7958781.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            complement(12574..13182)
                     /locus_tag="IVY70_001652"
     CDS             complement(12574..13182)
                     /locus_tag="IVY70_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP7958782.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(13188..14216)
                     /gene="vanB"
                     /locus_tag="IVY70_001653"
     CDS             complement(13188..14216)
                     /gene="vanB"
                     /locus_tag="IVY70_001653"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP7958783.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            complement(14209..15180)
                     /locus_tag="IVY70_001654"
     CDS             complement(14209..15180)
                     /locus_tag="IVY70_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP7958784.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            complement(15177..16004)
                     /gene="vanW"
                     /locus_tag="IVY70_001655"
     CDS             complement(15177..16004)
                     /gene="vanW"
                     /locus_tag="IVY70_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAP7958785.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVFADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            complement(16022..16828)
                     /locus_tag="IVY70_001656"
     CDS             complement(16022..16828)
                     /locus_tag="IVY70_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HAP7958786.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
BASE COUNT         4914 a         3756 c         2970 g         5340 t
ORIGIN      
        1 cttttagaaa aagcaaaaaa aaaaagaaag aaacttattc ttgctaataa actacgcgaa
       61 aggagtctaa cgttgtttga tataacttag actgatcgtt aacttatttt ttatatttct
      121 acatagttat ctttagaacc atttcagata catatgaaac aattacatat gattttttca
      181 taaaaaaaca gtactgtcta gtagtataaa atatacaaat ttcttaaaaa ttttttcact
      241 aaaatcacta cctgaaacag tctaaaacac ttatatatca acgtttaatg aaactatctc
      301 cgtttcattt cagatacaaa taaatatagt cgggctccaa atccgaaaaa aaataccccc
      361 agtagcttat cctaagctac tggagatgta taagtacggt catataacaa cggggtaggg
      421 taaataaatg aaaaagatgg aagaatcatt attcaataac ttttttaaat acttcgttgt
      481 aatcagttac ctgtccgttc ttaaatacca cagaataatc ttttttccca ttttcaagta
      541 accgaagtgg aatatcttta ccaactttct ctgtcactgc attactgata cctgttagat
      601 tagctaataa atctgataaa gattgagcat ctgtagaaat ctctgtatca gtatttatcg
      661 tgattccttt atcttcattt acaagtttcc aagtaggtgg tatagctgtg tatgtttcta
      721 caaatgaatt aagtttttca ttaagtgact tctcttttat atttccatct aaaaactctg
      781 tatatatggc ttgagctata ctaaaacaaa gctggccttg ttctaataat tttacatcat
      841 tagttagtaa agctgtgttc acgttatcaa tcgcttcaag aagagtatct ttggtgtccc
      901 taacagcttt aggtgtgcct tctggaattt ccctacttga tacctcgtcc attttatctt
      961 ccataaaccc cttaaaatca tatctttctc ccaaatccat agaagttttt gagaaagctt
     1021 tcatttctga gtctatttct tcaataatat ttaacatgtc tgatacatat tgtttaggct
     1081 gattcgtttt taatctatat gtggtaaata tctcaattcc tccaattatc aataaaattg
     1141 ccaaacaaat ccataataat tttttctttt tcatttttcc tcctatacac tgctgttata
     1201 acagtgacta atgtaaagat tttcccagca atcatcagac tattatttac agggaatcaa
     1261 aaaaactcct tagtaagctt acacctttag gagttcctgt ttgaacggta tgcataatac
     1321 atttaaatca atttaacatt aacacctcag tatatggtat ctctacatat acacggtctt
     1381 tttttggata gtactcgaca tttatattga tagcacttcc attgtctaac gtagctttag
     1441 cccggtacgt gttatcacca ttatcatgag tgatcgtcac atcatcacat gtccgattta
     1501 agttatactg ttcttgtaaa atctgagtta ccaatggtac agatgacttc tctagtccag
     1561 atgaacccgt cattcttgaa aaaattatac ctataatagc caatacaacg atacctataa
     1621 tgacaaactt cttattcata tttactttat tcaacttttt atctcctatc tccaaaatga
     1681 ttttctctca tcagtaactc attgacatcc ccaatagtac accaaagatg gctccagcta
     1741 cccctgctat catcaaaatc gtacccattt gttcatcttt ctctcgatat aagtaaccca
     1801 taataacagc aacagcacca aataccacag gaataaatgc taatgatact accatagaaa
     1861 tccaaccaat aatcaaatac agcttccctg tctcttgtcc atgtacgatt tgttccttca
     1921 tttcattaat tgtctgctca gtacttttct ttactgtgtc tgttccggtt gtctcatctg
     1981 tttcttgtaa tgctccgcaa aactggcaat acttactttc atttgttatt tctttaccac
     2041 attttctaca atacatgtct tctcctccta aaatcattca attgcttatt agctaactgt
     2101 ttactttatt aaatacatca gaaaacagct catttattgg tacacttaat atctcagaaa
     2161 gcattttcat ttgatagggt ttaggaatcg ttctccctgt ttgccacgaa cttacacatt
     2221 gctgtgtaac attcattttt ttggctaact cagtttggga aagtcctttt tcttttctgt
     2281 atcggctgag attacttact tttttcatga ttctcccctc ctgttttatt atatacaaca
     2341 atatgatgta tttcaactca taaaatatat aaatacttca aatgtcatta caaatacaac
     2401 aacttgttgt actctactga gaaggggggt gtattaatga aacagaagca atatgcaaag
     2461 gtcattagag aaaagcgaaa agcactcggg tggacacaaa aagaattagc taaaaaaatc
     2521 ttctctacac aacaggcagt agcaagatgg gaaaattctg taacagaacc taacctcgat
     2581 agcttaacag ctctttctag agccctagga acccctgtaa gccattttct agacaatgtt
     2641 gacatagatt atgaggaaga gtttttggct ctctatcgct ctcttagtac tgaagatgca
     2701 attcgaacaa ttgactatat gaagctatta aaaaggcaag aaaatgaacg caatcagcta
     2761 ttgaaagatt gaaaaataaa taacccaccg aactttaacg gtgggtgtat tgagatgaaa
     2821 taagattaag agctatggat aacctgtcta gtttaatata attgggtctc ttctattcct
     2881 tactctccgt ccatcatctc ttgttacact tgtaaattga taaataggaa ctaattcaac
     2941 tcctgataat atcaattctt ttattacttc atctatccct gcccctttcg ctagatatac
     3001 taaaaaacta ctgcttgctt tcacattgta tagtttctta atatccttag aagctcgttc
     3061 aagagtgaat ccttgtttaa caaagtagcc tctgttttct attaaagcat ttttcacgct
     3121 gttatccctg acatttcgta ggcgttcaca aacagtgtta ataagttctt tttctgtcat
     3181 tagatatgtc ccttgccacg ataagccctg taaaacttca tgtgcttcat ctaaaatcaa
     3241 ttgtttttcc ttgctaagtt ctgatcggga attttgagga aatactcttt tagcctcttt
     3301 ttcgttaaaa catatcttaa cccattcata agaaaaatat tgatatgtaa aatgaaattt
     3361 ttccagaagt ggaaggatat cttcattcaa tttttgaatc acagaagttg cattaaaaac
     3421 gtctaatcga tagacattgc ttctcttatg tttaaagttt cctaacgctt tctcttcctt
     3481 taacctattt aggtattcga gcatcttcaa aggaacatct tcatcatcta atttttttat
     3541 caatcctata aaagtcataa gtgatagtaa cttattacat ttatctatgt cataagtttt
     3601 accatagtaa gaactcattt ttttagctaa ttgttccccc gacaaaatac aagtcacact
     3661 ttccttacta tcatcaaaaa aattaacatc agaaagcaag acatcataga cacgtcgcat
     3721 ttcctctaga taattcctta ttgtcctgaa aagtttgtct ttcattgatt caagagtgtc
     3781 aaaaagtact tttgattcct ctttcaattt tcgaaaatgt gtaggataca agtctaggtt
     3841 ctctaatagc caattaaatg tttctacccg actgtctttc tttaaaatta gcatcacaac
     3901 tccaataaaa ttttcactga tatcacaatt actggaatga caataaaaaa tggaatgccc
     3961 agttttactg gtaaaaatac tagcagaggg attatgatcg ttgtgaaaca gacatcttaa
     4021 gtttttactt gacttaagta atttattcat atcaatggtt aacaatcttt cataaatctc
     4081 attaaaagac atgtcggagg aatcaaacag gcagtcttta aaccactgtt tcatttcttc
     4141 tttgtcgtca ttttttatca acttaacaaa atcggtttca ttgactagtt catactctct
     4201 ccctgaatca ctttgacaat ttttagaaca tgtcatggta aaatcaatcg gaagctcagt
     4261 aatatctaac atgttgtctt cattaattat tatcactgca tctgacttat atcctccaaa
     4321 aaacattcta ttcgaagcat ttacattgtc atcacacccg gggatgttct ttttcaagta
     4381 ttcgtaaaca gcaaccattt taccatagtc agtaattgct tcttttaata gaaagacaat
     4441 cttgtatttt tctagatttt cagagtaccg aatacttttt tgtatcgcaa acgcagaatt
     4501 atgaactaaa ttgatagata aaaccttttc tatgcttaag tatctactat catctttact
     4561 caaatctact accttcccct tctcatcttt aacagtgtta tcgacatcca gaataattac
     4621 tctggtttcc acaaaactct ccttgtcatt gcctttattc tttgtcttgc ttggaataaa
     4681 tgattgtgat gaatttagta tctctacaaa ctcatctctt gtaacttctc tatagttatc
     4741 taaacaccta tctcttacat cagttttttc tttttttgta actggcttct ctaataattt
     4801 ttctttatct acataaattg gaatattaat cattgatgtt tccatttgtg tttcctcctt
     4861 ataaaattct gttaaagact aaaacataca gataataaaa acaaactctt tattactata
     4921 tgttttagtc ttatttgttt actcaacctt tataccgttc catgaaacta tcaatatcag
     4981 attttttaaa tctaatgacc gtatcaattt ttataacagg taacccgtat tgagaaacat
     5041 attttctaat cgttcctact gatacaccta gatattcact tgcctgctga tagtccatat
     5101 actctagagt gactccagaa ataatgtctt ctagcatagg ctttagcact gtaattatct
     5161 ctttcacttg ttgttcaact tgtatagaaa caatgctatc aatgatttgt agagacatat
     5221 ttccccctcc tttcgtgatt gcttgaacaa attttctaag aattatcttc gtattttttg
     5281 ctcaaaataa aagtattttg ttattaaccg attaatcaaa caaaaaatga ttaccagttc
     5341 atcgacatac acataatacc tcgataaaaa aggggtgtca atagttttgt aaaatttatt
     5401 tacaacaccc cttttttgag gtatactaat tttaggaggt gtggtcaata tgataaaacg
     5461 tccaccaata aattatttag aacgaaaaaa gatactcgga acaaaaataa aagctataag
     5521 aaagagtaaa aaattaacac aaccagcatt tggtcttatg attaacaacg gtcaattaat
     5581 tgacaaaaag actatctacg aatgggagaa gggaacctat ttaccaatac cagagcgatt
     5641 atcccgaatt gctgatttgg gaaatatgag tatagaggag ttagtttgtg gcaatgtaga
     5701 agaatatatt ttgggaatta ttctttatag agatagcatt gtactagatg gtataacatt
     5761 tccagataaa aacctgttcc agcatcttcg tcaacaggtc ccaccagtac actctaattt
     5821 agatacatgg ctggatagat attccaaatt agaaccagag atgcaagaat ttattgctaa
     5881 caagacttgt aataaagtta aaaatgaaaa aattagtctt ttcaatatct taaaaataga
     5941 agaattattc atcaatgcta ttgtagaaga atttgacaat aacattcttt tcctcactag
     6001 cagtatagaa gaactactag aaagaatggt agatgaatgg ctacctattc aattgaaaga
     6061 catgtcttat cccgaagaag ctgtgagaga aattactgat aatataaaca agctagaaca
     6121 aacgatttca tcaattggga aaaaatacac taagaaaaaa atgaagggag gtgataccat
     6181 ctagtggtaa gtactccttg taaactaaga ggagatgatt tcatggcaaa aatatcaaag
     6241 tacaaaaaga aaaacggtca agtggcgtgg atgtataaag gacacattgc caccgatcca
     6301 cgaactggtg aaaaaataaa tactacgaga agaggtttta gtagcaaaca agaagctcaa
     6361 aaagattatg acgagtacag acatcaaata ttatacggtg ttaaaaagaa agcacctgat
     6421 atgacatttg aagacctgta taatgaatgg ataactcatc agcgtaccag tgttaaagct
     6481 tcaacaatcg caatctcagt aagatacgca aataatcaaa tacttccagc atttggtaag
     6541 cttaaactat cgaatatctc tgtcccatat tgtcaaaaag tggtagatga atggcacagt
     6601 aaatatgaaa gttacgatta tatgagaaaa cagactgcac aaattttacg ctacggggtt
     6661 gcaatgcaat atattgacaa taatcctatg gaaaaaacac ttcttccacg aaaaaaagag
     6721 tatgagaaaa atcgtaaatt ttactcaaaa gaggagctta ataatttact ggatgctttt
     6781 aaagattttg gtaacatgaa acaatacgcc ttcttcagat tattggcata cacaggtatg
     6841 agaaagagtg aagtactcgc tttacagtgg aaagacatag acacgtttaa taaagaacta
     6901 catgtcaata aaactcttgc tgtagatgaa tttggtaaag ttataattca atctccgaaa
     6961 acaagagctt ctagacgagt tatctcttta gatactgaga ctctttctat cctcaacaac
     7021 tggaaattac agcaaaaaga agagtattta aaacttggat acaatactag ctctaaagag
     7081 caacatgttt ttacaacagt taaaaacact ctatacattc caaacactgt taatgattgg
     7141 ttaagatata ttttgaaaaa atataacttg cctcgtatta cacctcatgg gtttagacat
     7201 acacatgcta gtttattatt agaagcagga gaatctgtca aggtagttca acaacgatta
     7261 gggcatgaaa atagtaaagt gacattagac atctatgcac atattacaaa taatgcacct
     7321 aagaaaacag gtcaagactt tgctgatatg atggctcatc agtaaaagag ctggtcaaaa
     7381 tctggtcatt tctagtatac aggcaaaaca aaaaagctag aacccttata tatcaagggt
     7441 tctagctcct ataacaggtt tacccaacac tgccttccaa tgactctttt gggaatcaga
     7501 ggaaagaatc aggctttata gagatatttc atctaacaaa ttactttaga ctgacagtat
     7561 atatcagcag gtactgtcat cgggaacgta gccaatatcg tatccaaaac agcaggtaga
     7621 gaagctattt gaggattcaa gttattctat ttcaaccatg aagttaagtt cattaaaaat
     7681 aaagatagtt ggtatttcaa taatttttgt atagagttaa tcactttatt gatgctaatt
     7741 catcttgatt ggtattataa tcgtattcaa gttgcgtggc gtgcttttgc tagaaaatat
     7801 ataactgttc tttataaaga aatataagga ttaaacattt tttatcatag agttttactt
     7861 atctatagaa ttatgtctgt tcctaattca gtttgatgaa ttaactgata aaaaaatatc
     7921 tactatttaa agatacaggg ataagtaagt aaaaaaataa catgtcatta gatagtagac
     7981 agttccaact ctttatttac cttttttgta tgctgcactg tcaatccaag ttcctaaatt
     8041 gttgtatcca tcatcactta tataatcata ttggccatca ataatttcgt caaaccacaa
     8101 atattctcca tctggttttt gcccaattat tttaattact gattgactag tagttgtcat
     8161 ctggttaaaa tcttttattt tacctatatt aacggccaaa atagcatttc ctcgcttata
     8221 actctcctgg atttcatatt taacatattc tcgatcaaga gtttctttac ctatcagcac
     8281 cacagtcact gatgttccaa ttaattgatt atctatccag ttttttatag caatatctcc
     8341 attacgttta actttctcaa attcagcctt atctacaaaa ccagcttcct ttgttcctcg
     8401 caaagtccag ctgtttctaa ctaccattgc tcttgttaca tctgcataat ggaaactaaa
     8461 aaaaaccctt ctcaccatat taatcctcct gaatactatt tattaaactg acaatttgtt
     8521 caactttttt atctacttct ccttcagtat ctaatttttc tagcgcttga aatttacttt
     8581 ttaaggaatc atcaaaataa tcactgaaat tctcttgaat cttatcaaac aaaaccttac
     8641 ttgcaccttt ggtaaatcca atcggaacta ctatattttc attttgaaca gataattcaa
     8701 attcttcaat cattccatct gctgtaacta tttcacctga ttcaagtttg tttccaaaaa
     8761 caaaaatact aatcccagac tctgcaataa tgttttctcg atatcttctc cactcatgag
     8821 gcatatcaag gggaagtggt cttaaagaaa gtacgtcttg tatgttattt tttcttaaat
     8881 ttcttctctg ctttagaact cctgaaacaa tactattacc aattccaagt ccgtaacctg
     8941 atactattct ataaccggat tcagaaagcc tatatgctaa tttctcgaca aattttaatc
     9001 ctttcgattc agatgtaaat ggagtataat caacagcact tcccgaaata aaaatagttt
     9061 ttctcaaata cttttttcta atacttttca aaatatcagt aatctcagaa taatcatcta
     9121 cttcaacaac gtttatcgaa aatctggaca aatcattgat ttgtagtctt ctttttgtca
     9181 gttcataatt atatctctct tgatcaaaaa aatcctcaga atcgacaaaa ttttccttta
     9241 aaattatatg ggattttaga ttatcttttt agaagttctt gaatgtcggt atagcccgtg
     9301 tttccgggct tttccggcat ttttgatatt gttagatact tacaaatgtt cttattacct
     9361 ctcaccattt ccccctcaaa agtagtaaat ggagtagtaa aaccggcctt gtgtcaggct
     9421 gccagccgtt ccatttctgc ccttgcggaa tcgaatgtgg cgtgtgcata atagttcagc
     9481 gtcatggtga tattggaatg tcccataatg tactgcaagg ctttggggtt cattcccgca
     9541 ttggctaacc gggtgcagaa tgtatgacgc atagcatggg gcgtcgtcgt tttcggcaac
     9601 gcttcttcat ggctcttgtt gtacttcttc acaagcctgc caaacatccc gccataatct
     9661 accgccgtca tgggatagcc gttctgtttg agaaacagga aattggcata cccgtcaata
     9721 atgaacggct ttgcaccttt gcggtttttt atcactcgct ggaacgcctg atacacttcc
     9781 tcattcatgg ggatttttct gacgccgctt tgggtcttgg gttcgtcaat atagtaaccg
     9841 acgccggtat ttctcaaaag ctggtggctc acgatgatta cccggttttc aaaatccaaa
     9901 tctttgtccg tcagtccgca cagttccgaa atgcgaagcc ctgtccccag cagaataacg
     9961 attgcgtcat aatgcttttg gtaaacctta tcaccttgaa taaaggacag aaaactttct
    10021 tcctgtgctg gtgtaagagg cacctttggt tccgtatcgt cgtccagaac gtcactaagc
    10081 tggaagtcaa acgggttttt tcggatgcaa tcgtcctgta tggcggtgta aaaagccgct
    10141 ttcagggaac gcttgtcgtt attgatggtc ttataagaaa ggcccttttc tttcatccga
    10201 agcgcccatt cctttgcgtc ggacagcttc acactgtcaa tcgggcagct ccccagccga
    10261 tcttgttcca gaatacgcat cagccgttcc cggcccagtt tggtgttgtg cttcacattg
    10321 ccccggtgcc tgatctgctt ggcgtagagc tggcagacgg tcattttctt gccgatggtg
    10381 tctatcccgt cgtcaaggtc tttctggatt tctttctcct tttcccggag tgaaatatcc
    10441 tcccgtttac ctgccggggt cttatccgta ggcactaact tccatgagta aacaaactgc
    10501 ggttttccaa aggtatcaac atatttgtaa gcatatcgtc cgtcttttct ctggctctct
    10561 ccagaccgca aaacgcgatt tttgctgtct cgtctttttt ctgacatctc gtcatgctcc
    10621 tttccatgac ggaaagagcc gcatatatgc ttataaatta ttataacata tctgcggccc
    10681 tttttcacta ttagattgtg tccaaagtgt caattatttt ctcaaactgc ttgcgtttga
    10741 tctggatgcg gttgccattc ataatgaccc agccagaggc cggattttcc tcggccagct
    10801 tacgcaattt gttttctccg atacggaaat atttagacgc ttcctcaatc gtcagcgtgt
    10861 atttttccca aataggcaca tcatggttca taatgcagcc ccctttcaca aattcaaagg
    10921 tttgtgtgat ttaggggcat agacggacgg ctgctggcac agctccacgg gactttcacc
    10981 cccgcgtggt tctcacgcgg ccctacccat tgcctgcgac gcttttaacg ctcggactgt
    11041 ggctataagg gagtatcata gtcctgcctg tacgtcgtcg cccgcaagcc gccacgctca
    11101 cattgcggcg tggtgttcct cgctccgctt ttcccgtagg gaaaagtatc atggcgcacc
    11161 agccgtccgg ctcggcacag acagaatgtg tccgtatgcc ctatgatgcg ccgccgttat
    11221 cttgcgggca tatttatcaa ggtgcgatca ggccaccggg aaaacaccga aacggggaaa
    11281 gggtaagccg tttcatgctg ccggggccgg ttcgtcagtt cgtgacgata gcgtggatga
    11341 gcttgatttc cagacggcgc ttcatggcct cgtccagaca cacatgcgga tagccgtttt
    11401 cgtcatagag cgtccgggta cacagcttgt tcatgtaacc gctgtaatac tgcaaaacgc
    11461 gctctacggc tccggcatcc ccagcgcggg cggcctcgat caccgaaagg ggcaaaagct
    11521 caatgccctt gtagttcggg ttcttcatcc gcgtctaacc tcccttcggc atcaacgcct
    11581 ttagctgttt ccgcagctcc gataaggtat ttgtcctgtg ccgctgaacg gcactccggg
    11641 acattcccac aaggccgccg atctcaccat ctgccatatc cagaacacag tgcaggatta
    11701 aaatactctg ctccatgctg ggcagcgcgg caaaggcttc ggcaacaagc tcattgttga
    11761 tatgtaggtc atagccatgc gacgaaaacg taaagctgtc gctggggtag tgatctactg
    11821 tacagagctt gtccaaatct gcctgtgaca aagagcttag tgacacttca cggtcacggc
    11881 ggcgcttcat ttcagccaag tagcttttgg cctcgttacg cagtaccgtc ttgcaaaaag
    11941 catcgaacat gcaccgcgct tcatagtcgc gggggatagc atccatcttc tcaccccctt
    12001 tctgtggggg acttaggtgg ttctctccct ttccgctaac aagactttga caaggccgcc
    12061 agctacccgc taaatacctc ctttttgaaa atttctcaaa aaattttttt ggggacagcc
    12121 gggccgcaaa agacgacaaa aatcgcccgg caggtcgcaa acctaccggg cgagaaaatg
    12181 ctttgctatg ctgctgattt acatggtata gtacatactc ataaccggaa tagccctgtg
    12241 gaggggcaag gctttttttg agtggtggaa gtggattaga gcatggcaat agcaaaacag
    12301 catagcggca tcctcctatg ggccgccaca ccttttgctg ccagaaaaga acggcggcct
    12361 tgatttctca aaaccgccgc cagcaaaata caaaatgggc gtgggaaagg tctgcccaac
    12421 agcggttttt ttcttttctg ccgctgggca gattgaatac tataatttgc aatgtattac
    12481 aaaagatgct ttaaccactt tccagtaaaa gattgtccat gaccagcata gattttatca
    12541 aatccaaaat ctgatacaaa accaatactt tcattatgaa acggcaaaat taaaataggt
    12601 atcggggtat ggctcatcaa tcaacttata gtgccaccat tcaaagcgat aagactgaaa
    12661 tccgctgctt tccatgattt tacgcaagca tcttcgattt tgtgcctctg catcccctat
    12721 cccttttgct gtatgatgcg accgttcgtc cataaaatcg aagttacttc ccattgaaac
    12781 aagttcccct gtatccaggt ggtagagcgt aagatcaatt gcgcttccac ggctatggct
    12841 ggattgtgag gccacataac cctttgtaat caactcggct cgctctatat tggggtaaaa
    12901 tttttctttt gtgagattgt cctccggttg cgctgcccaa cgcaggaagc agtccaccgc
    12961 agttcttggc cgatatccat cccataaaag caagccatag ccaagcgcag ccgcatggat
    13021 ttgtgcctcg cgcagagcaa acgccacggc ctttgtgccg atgatgcgat tcacctcata
    13081 cccatccacc ggttttcctg tgaagttatc ccatgtggcg tacttggcat cccaacggac
    13141 gccatgcaac atttcatcta aaaacaaaaa accattttcc atacttatca cctctttaac
    13201 gccaatgtaa tcaggctgtc aatcagtgca ggaagcgtga ttcctgcggc ggccatcata
    13261 cgtgggtagc ggctgtacga tgtaaaaccg ggcagggtat tgacctcatt tagaacgatg
    13321 ccgccatcct cctgcaaaaa aagatcaaca cgggcaagcc ctctgcatcc aagcacccga
    13381 tatactttct ttgccgtttc ctgcacccga tttcgttcct cgaccggaat gtctgcggga
    13441 actgtaatca tcgcattttc tgagcctttt tccggctcgt tttcctgatg gatgcggaag
    13501 ataccgtggc tcagccggat ttgatccact tcgccgacaa tcaaatcatc ctcgttcccc
    13561 atgaccgcac acccgacctc acagcccgaa atcgcttgct caattaagat ttttccatca
    13621 tattgtcctg ccgcttctat cgcagcgtta agttcttccg taccgtttac tttggttacg
    13681 ccaaaggacg aacctgaccg tgccggcttc acaaagacag ggtaggtaag cgcacccgcc
    13741 tccggcttgt cacctttatc aatcatttga aattcgggaa cggcgatgcc cgcatttttt
    13801 gtaagaatgt aggccagtga tttgtccatg caagctgcgg agctttgaat atcacagccc
    13861 acatagggga taccagacaa tacaaacagc ccctgtatcg caccatcctc cccgcatttg
    13921 ccatgcaaaa ccgggaaagc cacatcaata cgccgtgttt cgtattcgct ttctttcatg
    13981 acaagcagcc catgcgtttt cctatccggg gagagtatgg cggggagact gtcggcttcc
    14041 cattccgtac atggcttctt gcatagcttc catacaccgt tttttgtaat tccgatgtag
    14101 tgcggatcga atttttccgt atcaatgttc gcagcaattt ctattgcggc ttttaccgac
    14161 acatcatgtt cctccgagca accgccgaag atgattgcga cttttattct attcatgctg
    14221 taaactcctt tcaaaattga gacaattcct gattgttttt tctgtagtat cctgcagcac
    14281 acgctcagtg tagtacgccg tatggggtgt gatgatcaca ttcggcatcc ttaaaagctg
    14341 cgacaaaaag ggatggtcaa gcactttctg cgagcaatcg gtataaacaa actgatcctc
    14401 gccctccaac acatccagtg ccgcaccacc cagctttccg ctttccaacg cctccaccag
    14461 cgccccggta tcgacaagcg ctccgcgccc ggtgttgatt aaaaatgcgc cttgcttcat
    14521 ctctccaatt tgcttctgac cgatcagatg gcgggtatcc gcacaaagcg gcacatggag
    14581 cgtaacaata tcgctgtttt ttagaagctc atcaagctgg acataatctg catccatttt
    14641 tcggctgtta tcataggcca gcacatggca tccaaatccc cgcagccttt tgacgaccgc
    14701 ttgcccgata tggccggttc caataactcc cacagtcata tcccccagtt ctttcccccg
    14761 gatacgatcc aatctgaaat cttgttgcgc cacggcgtgc atggtgggtt ttgcaccccg
    14821 tatggccatc agcatcagca tcaaagcata atccgcaacg ctgtccggcg aatacgcaac
    14881 cgtgccaacc gagattccca ttctctcggc ggcagtcgta tcaatgtgat cgcagccgat
    14941 gctgcgggta gaaatgtatt ttaccccgac ctttctcagc gcaagaattg tcgcctcgga
    15001 aacctcggac ttatggctta cgctaacgca ttgattgcca gcggccaatt ttgcgttgtc
    15061 tgccgatatc gcatcgctaa tcagcgtagg gataatatga aaatccggcg ataaagcgcg
    15121 gaaagcattt gcctcatcct gttcgcatcc aaaaacagta atgcccacac tttttctcat
    15181 tggttctcct cctgaatatc cacactttcc gggaggggat aacagatttt gcatttgttt
    15241 gtataaagca gcttgcaatc tattatttcc ccggtaccta aggcaacttg catccgttta
    15301 acctgggcat attcataaat cccgccactt tcacggacat actggatact gccatttgta
    15361 attttgtata atgcttgggg ctctttgtcg gcgaacacct gaccgatgat tttctcatca
    15421 tctaaggtca cccatatttg gtaggtgcag tcggtatcgt ttcgcacttt taaatcaatc
    15481 cagccctctg aaatggttgc atccacccct ttaatctcgt cactgtttgg ctctggaaac
    15541 tcctttactt cgtgacctct gcgctggata attgtcaatg gcgtatgcag gaacatccaa
    15601 aatagtaaat tgctcatctg gcacataccg ccgcccgaca tggtggtgag tttaccattg
    15661 gtcaccgtaa ggccgtcttt atagggggta tctttgtccg catggcgtac cagccaccag
    15721 aaagaaaagg tttcccccgg tcttatcaat aagccgttta aggtctttgc tgccagcttt
    15781 aagttgaaca ccttgttttc ctggtatatc atgtcaaatc cggtattgtg gttgtataat
    15841 gcgcaatccg tttcaaagag caaatgggaa agcgattttt cccctatcgt ctgcgcatag
    15901 cgacagccgt caaatctcat tcccgcataa aagcacattt ttctctgcgc ccgtctcatt
    15961 ggaagcagga acgggaaacg ctgtgtcaat ctttttctgt ccataacata acatcctttt
    16021 ctcatttttc tggttttaaa tattcctcaa ggcaaacccc ttggttgtac atctctgtgg
    16081 cagcttcgat gccaacatat cggtaatgcc acggctcgtt gctcacaccg gttatctctg
    16141 tcttgtctgg tgggtagcga cgaataaaac caaaacgata gctgttttca tccagccatc
    16201 tataaacctc gttgcctgtt gaatgaattc cgtccgcatt gatatccaca gcaagaccaa
    16261 gctgatgctc gctcgttccc ggcacggcca cccaagtttc tgcttccgct ttagcctgtg
    16321 cagaggtgta gcctttcgcc ttatattcgg caattttttc atccataatt tcttgctgtt
    16381 tttctgttgt tcggtatccg gatgcgacaa tcgggtaaac tccatcagtt cttgcggcat
    16441 caaacaaatc ttgaagatag ggagaaatcc gaatatctat ccgctcacca tttgatagtt
    16501 gctcaagctc cacatcgtac tgtgcgggga tggggttctg cctgttcact aaaatcaggc
    16561 tccattcctg ttcatctccc tgttctttgg cggtggcaag agcgttttcc tccaaattct
    16621 ccactacgtc agtgctgctg cctgtatgag ttgcagggat gggcggtatc tgtgttgcct
    16681 ccaatacgga agccagtctc caccccaaaa acagcgtgca gactgtgaac gaaataatga
    16741 acgcacatag aaaagcccgc ttttccacgg attgtttcat atgccgtttg tgatgatccg
    16801 cattggaatg atagttgctt ttttccattt gatcgaatgc ctcctttgca ataaaactac
    16861 gatttgtggc tggtacgatt gttattatgg caagcgcatc tgtactttcc ctgtagccag
    16921 tctgtagttt tcctgtagca gcaaaaaaag accggcaggg ttatacctgt cggtcaaaat
//
LOCUS       DACCXO010000055        16857 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.056, whole genome shotgun sequence.
ACCESSION   DACCXO010000055 DACCXO010000000
VERSION     DACCXO010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.056"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            44..1045
                     /gene="ccpA"
                     /locus_tag="IVY70_001657"
     CDS             44..1045
                     /gene="ccpA"
                     /locus_tag="IVY70_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP7958787.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(1382..3727)
                     /locus_tag="IVY70_001658"
     CDS             complement(1382..3727)
                     /locus_tag="IVY70_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP7958788.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            4006..4908
                     /locus_tag="IVY70_001659"
     CDS             4006..4908
                     /locus_tag="IVY70_001659"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP7958789.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            5121..6896
                     /locus_tag="IVY70_001660"
     CDS             5121..6896
                     /locus_tag="IVY70_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP7958790.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(6962..8548)
                     /locus_tag="IVY70_001661"
     CDS             complement(6962..8548)
                     /locus_tag="IVY70_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP7958791.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            8799..9023
                     /locus_tag="IVY70_001662"
     CDS             8799..9023
                     /locus_tag="IVY70_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP7958792.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            9165..10916
                     /locus_tag="IVY70_001663"
     CDS             9165..10916
                     /locus_tag="IVY70_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7958793.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            10916..12700
                     /locus_tag="IVY70_001664"
     CDS             10916..12700
                     /locus_tag="IVY70_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7958794.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            13026..13877
                     /locus_tag="IVY70_001665"
     CDS             13026..13877
                     /locus_tag="IVY70_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7958795.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            13931..15682
                     /locus_tag="IVY70_001666"
     CDS             13931..15682
                     /locus_tag="IVY70_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7958796.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            15704..15856
                     /locus_tag="IVY70_001667"
     CDS             15704..15856
                     /locus_tag="IVY70_001667"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958797.1"
                     /translation="MLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDA
                     LITALT"
     gene            complement(15896..16798)
                     /locus_tag="IVY70_001668"
     CDS             complement(15896..16798)
                     /locus_tag="IVY70_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7958798.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
BASE COUNT         5264 a         3049 c         3470 g         5074 t
ORIGIN      
        1 cgtattttca gaaaacatat aaaatatgaa taggagaaca caaatggaaa agcaaacaat
       61 cactatctat gatgtagcga gagaggcaaa tgtttcgatg gctactgttt cacgtgtcgt
      121 aaatggcaat ccaaacgtta aaccagcaac aagaaaaaaa gtcttggaag ttatcgatcg
      181 cttggattat cgtccaaacg cagttgcgcg tggcttggca agtaagaaaa caacaacagt
      241 tggtgtaatc atccctgatg tcagcaatat gttctttgct tctttagcac gtggtatcga
      301 tgatgtggca acaatgtaca aatacaatat tatcttagca aattcagatg gcaacgacca
      361 aaaagaagtc aatgtattga ataacttact ggctaagcaa gtggacggcg tgatcttcat
      421 gggtcaccat atcacagacg aaatccgtgg agaattttca cgttcaaaaa ctcctgttgt
      481 attagctggc tctattgatc cagatgaaca agtaggtagc gtaaacattg attatgtatc
      541 cgcaacaaaa gatgcagtaa acaaattggc aaaaagcggc aataaaaaaa tcgcttttgt
      601 cagtggtgcg ttgatcgatc ccatcaatgg acaaaatcgt ctgaaaggct ataaagaagc
      661 attaagcgaa aataacctac catatagcga aggattgatc tttgaagcac aatacaactt
      721 caaagatggt ctatctctag tcgatcggat ccataatagc ggtgcaacag ctgtctatgt
      781 ttcagatgat gaattagcca tcggtatttt ggatggttta ttagaccgtg gcgtgaaagt
      841 accggaagaa ttcgaaatca tcacaagtaa caattcacta ctgacagaag tagctcgtcc
      901 tcgtttaaca agtatcacac aaccattata cgatttaggg gctgtatcta tgcgcctatt
      961 gacgaaaatg atgaataaag aagaagtcga agaaaaaacg attatcttgc cttatggaat
     1021 cgaagaaaaa ggatctacaa aataataaaa ggacaaccat tcagaggttg tgtgcctgcc
     1081 gtttaactct gcgttacaag gagcaatttc aaaaaacagc atctcatgtt tgatgaatat
     1141 tgtgactcgt gattcggagt tggcaggaga acaccgttta tttgggaaaa ggggttatga
     1201 caaaattttg ttataatcct ttttttgtgt cttaaaaaag agagaaagtg gttgaaatta
     1261 catttatcgt atataaaaca atccgtgtat agatgctgca ttttgttcac caccatcaaa
     1321 aaaataaggg actgtgacaa tactattgtc acaatccctt attactgtgt attctattaa
     1381 atcaatcatc tttctcatct gattctttag ttgcttgatt cgctcttgca taagctgaag
     1441 cggtgttcca gtaatctgta tagttttcat cggtaccgcc aacgccaaat ttaaaggcgc
     1501 ttttcttcgg tccatttttc gcccatagcg attcaactgt ttttcctggt gtttggatgg
     1561 attttttatt gacagtaact tttccaggtg tttgaccagt aaatccagat acttttttct
     1621 tcatcacatc agatgataat tcgaatttct catcagtgcc aaaaatgtca ggttttgctt
     1681 ggtaagcttg attgatcaga ttggcaagat aattagccga acgtatcctt gctttgctgt
     1741 ccattccagt attgtcatcg tgccctgccc agctactgat ggtaatagac ggagtagaga
     1801 caattagcca tgaatctcgg tattcattgg tagaaccagt ttttccgacc caatctgctt
     1861 ttcctagatt tccgtttaag cttgagatgg catctttgaa tggtgtagtg atttttgcat
     1921 tgatcacact acgcatcatg tcattcatga tcgatgccgt tgcttccgaa tagatccgga
     1981 cagagttgct ttcgtgttta taaattacat ttcctgcgtt atctgtgatg gaatcgatga
     2041 tatagccttc ttcatagacg ccgccgtttg ctaaagcctg aaatccgttg gtctgttgaa
     2101 gcgtcgtgac atctactgta cctaaaggag cagattcaac gccccaattg tcatttgccg
     2161 ggtagttcat ctttgccaag tagtgctcgt aagcatattg tttcgatcca ccggaattca
     2221 acacatcttg atataagtga taagccggga tattgttgga ccattctagt gactcacgga
     2281 cggtttggaa ggtgttcgat ccttcgttag tagcattgac gattttctcg ccagcatttt
     2341 ctccttcttg ccaagttgtt gcataatctg atacacggga ttctgatccg ataagcccca
     2401 tgtcgattgc aggtccataa acaagaactg gtttgattga tgagccagct tgacgttctg
     2461 tatcgaaggc atggttattt tgattctgac tgtaattccg tccgccaacg aatccgtaga
     2521 tgcggccagt tctgttatcc atcaatacat ttccggtttc tacttgactg ccagcaccat
     2581 catctaacat gtatccataa ttttcaacgc cattttgcaa tgcagcatag acatctttat
     2641 cgattgtcga atggattgta tatcctttat tttggatcgt ttgctgtgcc ttttgataat
     2701 aagcgtcact gaccgatgaa tcagacatat cagcgttgtc tttttcagcc aattgttgtg
     2761 caatgattct tgttgcttca ttcatcacag tataatacaa gaattctctg tcagtctgtt
     2821 ctgcgatttg ttgtggtaga aagtcttttg taaggtcata ggcttttgct tcttcgtatt
     2881 gttctttcgt aatttggcct tcacgataca tactgaataa aacaaaatct tttctggcaa
     2941 ggccggcaga aagatcttct tttagcgcgc ctgtgttcgt gtatggacta tacgtgatcg
     3001 ggctttgggg taatccagcg atataagcag cttgcggcag cgtcacatct tttgcatcta
     3061 caccaaagat tccttttgca gcttcctgga ctccagcaat gttttgtccc ttattattac
     3121 gaccaaatgg tgagacattc aaatacgtag caatgatctc atcttttgaa aagtattttt
     3181 ctacttgtgc agaaagaatg atctcgtttg ctttacgttt gaatgttgtt tcatctgtaa
     3241 ggatctgctg tttgactaat tgctgtgtca aagttgatcc accagaagag ccaataccag
     3301 tagcttcaga tactaatgct cgtaaaacag ctttaggaac gtaaccttgg tgtttatcga
     3361 agtattcatc ctctgtgctg ataattgcag ttttcaataa aggtgaaatc ttatcagatg
     3421 aaatagtcgt gcgcatcaga tctgtctgga tttttgatat ctctgtatta tctgcataaa
     3481 caatctttga cgtttctgta atgtttccta gttctgtctg cagtgctttt ttagtcggca
     3541 attgtgtgtc ttcgacgaga taggcaaaat agccagcgcc tattccaata cctagagctg
     3601 cgaacatcaa aagaagaaca gtaataaaaa cagtcaatga ttgaaaaact ctaaaaataa
     3661 tcttaggaac aaaccatttt tctttttttc ttctattctg tttttttcga gtatttttgc
     3721 gtggcaaaag ggtcacctca actaatggtt ttatttcgct aattatagca aataatagaa
     3781 caaaaaaaca gacaaggctg tttttggcag gaatttcttt tttaaaagag ttaaaacgtg
     3841 agaaaatgat ctttattata aaagagttct taagcttttc tttagagaaa atttcagtta
     3901 ctatggagaa cagggcaaag aaaaatcaat cattttcttt gtatatcatc ttaccaatat
     3961 ataggaattg ttagtaacaa ttgcctttca aggaggaatc catccatgtt attaggctca
     4021 cacgtcagca tgagcggaaa gaaaatgctg ttaggagcag cagaagaagc agccagttat
     4081 ggatcttcga cttttatgat ctatacaggc gcccctcaaa acactagaag aaaagccatc
     4141 gaagagatga atattgatgc cggaaaagcg tacatggcag aaaaaggtct aacaaatatc
     4201 gtcgttcatg ccccttatat cattaatttg gggaatacga tcaaaccaga aatgtttcct
     4261 tttgccgtcc aatttctgag agaagagatt gttcgtgcgg aagctttagg cgctactcaa
     4321 attacacttc acccaggtgc tcatgtaggg gcaggaacag aagccggaat tgctagaatt
     4381 attgaaggct tgaatgaagt tttgacaaaa gaacagacgg cacaaatcgc tttggagaca
     4441 atggcaggta aaggtacgga aatcggtcga acatttgagg aattagcgca aatcatcgat
     4501 ggagtaaaat tgaatgaaaa attatccgtt acttttgaca cctgtcatac gaatgatgcc
     4561 ggttataata ttaaagaaga cttttcaggt gtaatggaag agtttgataa gatcatcggg
     4621 ttagatcggc ttaaagtact ccatatcaat gattctaaaa atccgcaagg ttcgcataaa
     4681 gaccggcacg ccaatattgg ttttggaaca atcggattcg acacgctgaa taagatcgtc
     4741 catgatccaa tgtttgacaa tatttcgaaa atcttggaaa ctccctatgt aggcaaagat
     4801 aaaaagaaag cttacgcacc atatggaaaa gaaatcgcca tgttcaaagc gcaaacattc
     4861 gatccagaag tgtttcctga gttattagca gaagaagtga tactctaaga ttttctatct
     4921 gacagcagca aaaaaggacc aggcacattg ccttgtcctt ttttgtttaa gaaacaaagg
     4981 agtacttttt gcagtctttt tcatcatgca tcttctcccc cctcttttta aatttagaca
     5041 aacaaggtat actggtagaa aagtaaaaaa acgaaaagtt tcacaagcaa gtggcaaaac
     5101 gtgattggaa ggacgaaaag atgctgaaca ctttaaaacc cttgatcaac caactaaaaa
     5161 agaaacatac atactttatt cttggcatag ccctattatt tacaattatt cgtttgtatt
     5221 tatatagtaa agcaatctat ggaacagaca tgtcggcaac ctatgatgac cagctgctga
     5281 ttcgctatgc tcaaaatatt ttggacggta aatggctagg ggagtatacg actacaacac
     5341 tttccaaagg aatctcctac agcttatttt tagtacttgc acataaactg catctttctt
     5401 atgcagtttt gttcggcatg ttcagtattt ttgccagtat attggttgca ctcgcgttta
     5461 aaccgattgt gaagaacaag tatcttcttt taagtatcta tttttatttt ttgttttctc
     5521 cgattagttt tacacatgaa tattccactc gaacatatcg taatgccctc gtgattccgg
     5581 ctgttgagat cattatcgct tgtttattag ctatttatta ccgcaaatac ggttctagca
     5641 gaaaattgct gccatggttt ttagggcttt ctgtcgcttt tcctttcttt tggttcattc
     5701 gcgaagattc tatctggctt cttcctttta ctgtagtagc tttgggtatc agtttcgtcc
     5761 agatcttgct agggcaagac ttttctttca aagaagtaaa gaagttaaaa aaacgactct
     5821 ttcttctctc gaaacgggaa tggctgaaac tttcattgtt tttacttcct atagttggct
     5881 tttccatcgt ctatcaatgg atcgaatata aaaatgagac aacatacggt attgcaacca
     5941 tcaatgaccg ttccagtgga gaatttggac gtttgatgaa acaactgatc cgaatagatg
     6001 atgaaacgaa cttgaataaa gaaaacagtg agatatgggt ctcacatgct gcactaaata
     6061 aagcattagc cgcatctcct acattccata ctgtttctac ccgtattgac gaattatata
     6121 cgactcacgc gtggactcaa ggtggaaaag taaaagaatt acccggagat atcatctttt
     6181 gggcattgcg agatgtggta agcgaatctg gttattacca aaacaatgca aaagaaacca
     6241 atgatttttg gaaaaaagtc aaccaagaat tagaagaagc ctatcacaag ggaacaatca
     6301 aaaagaaaaa agagttctat ctgatgggat caggagatgg aaagacgatg agtgatctgc
     6361 caatcgtatt cgatttcctt caatcaggat acgcttatgc atggcgatat aaagatttcc
     6421 atcaaggagg aaattatagt ttcggaacgc aagaacaagt caaacaggct caagaattat
     6481 taaaccttcc gctaatggag aattggatcg atagtaatga accgagaaca cctaatttcc
     6541 aattaacaaa atcagcaaaa gtcgcgaatg tcatcataaa aatttaccaa gttctggcta
     6601 ttcccgtgct gattgcttca atagcaggga tattacttat gatactagga accgtatttg
     6661 caaaagctga tcgtgcctat tatagttcct ctttgattgt ggtcaccgga ttaatactga
     6721 cacaattggt ttttcttttc ggtgtctcat ggttttgttc ttatgcgcca gaaaaaaagg
     6781 actatttttt aagtgtctac actggtgcag gcgtgccatt aacgcaattc atcgtagtaa
     6841 caagcatgct agttttcagt aaattaaatc tgaaaaaagc tttatcttct aaataaaaag
     6901 acccttggta gctagatttt taacctaact atcaagggtc tttgtgtgga aaaatcagtt
     6961 tttattcgat ttcttctacc gtttcctgca aaagctgttc atattttttg tttcctttat
     7021 ataaatcacc aatcgtccat gccaacagga gaaggacaag aacaatgatt acataagcga
     7081 tgtgatcagc caatgctagt tgagcggaag tggcttgctc gccgaagaaa ctttcgatct
     7141 gtcctggtaa tcctcgtaag ttcaagtaag ttagactaat cacagagaac cagccaagaa
     7201 gttttacgat gaatgaattt ttaaatcggc tacccatctc cgcttggctg ttggtcatca
     7261 tcagtaaagg aatcatcgaa aacggcaacg caaaagccaa gaacacctgg gattcattca
     7321 ttaagttatt caaagcggca tgttcttcga tcgttccttt tgtacttgta aacaaaacac
     7381 aaatcaatac aggaatcaca gaaagcaatc gtgtgaccag tcttcttagc caaatcggga
     7441 tacgcatatg gatgaatcct tccatgatga cttgaccggt caacgtacca gtaatagtag
     7501 agttctggcc agaagcaaga agtgctacag caaataaagt ggacaagata ccagattttg
     7561 ctacagcaat caaaatacca ttgctcatag ctgatggatc agataaagct tcatacagac
     7621 caaaaaagga aggatcttgg acagaaccgg ttttgaagac agcgacaccc atgatcaata
     7681 gcagtgcatt gactagaaaa gccattgaca actggatatt agaatcccaa gtagaaaaac
     7741 gtacagcttg tgctacatct tcttcgtttt ggtgatcgat cttccgagtt tgtgaaactg
     7801 ctgaatgaag atataagtta tgcggcatga ctgttgcacc aataatccct aaagctccag
     7861 tcagcggagt ttcgccgccg atgaccggtg agtctgagaa tgtatgactg ttagggataa
     7921 agccagcaaa taaactgtgc caatctgggt tagacaagcc aacttgatag acaaaaacga
     7981 ataaaatgac gaaaattaag cagacaacca gtgcttcgat cttacgaaac ccgacttttg
     8041 tcagtagtaa gagaacgaaa acatctaata cagtaataaa gacggcaatc accaatggaa
     8101 tatcaaaaag taagtagagg gcgattgctg caccaatgac ttctgcaata tctgttgcca
     8161 taatggctaa ttcagtcaag atccataaaa tgacacctaa tgtcttactt gtgcgtgctc
     8221 ggattgcctg tgccaaatcc atttggctca cgattcctaa ttttgctgcc atatactgta
     8281 aaagcatagc aatcagacta gacatcaaaa taactgacat caataaatat tggaaatttt
     8341 gtcctccggt gatcgaagta gaccagttac ccgggtccat atacccaact gctactaacg
     8401 caccaggtcc agagtaagca agaagtgttt taaaaaaact tttccccttt ggtacatcaa
     8461 ctgttccatt tacttcagct aaagataaac catttgtatg ttcaataagt cgatgcttat
     8521 gaaattgttt attggattga ttattcaaga aaaaaccacc tttcctattt atctacgatt
     8581 acattctaca cctcaaaaag aaaaaattaa agaaaaagat tcggtacgcc taattttttt
     8641 tcatggcgcg tcttgctaga tgacgaaaga aattttgttc tcctctaaaa aaaccactat
     8701 tttttgcatg aaactaaaga atacgccttt cttttttcaa taaactttag taaaataagt
     8761 cattgagagc aattcggaaa tagaaaggag agatttatat gttatcaaca ggtattgtcg
     8821 tacttattgc tattattgct ttgctaatcg gcgcagtagg tggctttttc ctagcgcgta
     8881 aatatatgaa agactattta gaaaaaaatc ctcccgttaa tgaggacatg ttgcgatcaa
     8941 tgatgatgtc aatggggcaa aaaccatctg agaagaaaat tcgccagatg atgcagcaaa
     9001 tgaagaacca agggaaaaaa taggttctga cccaacgact ttttacgggc tgattttttc
     9061 agtccgtttt ttatttcgta atttgttaga ctattcagat tattcagcaa aaaagacaag
     9121 cacagacaat cctaggctct tgataaagac aaaggaggag taaaatgtcg atttttagaa
     9181 aattaggctg gttttttcgt caggaaaaga aacattattt aattggggtt ttctcgctat
     9241 tcgcagtggc cttggtacag ttagttcctc caaaagtgat tgggatcatc attgatgaga
     9301 tcgccgataa aaatttatca ataaagatca ttttcgggtg gatcttccta ttgattatcg
     9361 ctgctttttt acaatatctt tttcgttata tctggcggac gaatatttgg ggaagtgcag
     9421 ctcgattgga gaaagaactt cggcagcagt tattcaaaca ttttacagaa atggacaatc
     9481 tcttttacca aaaatatcgg actggggatt tgatggctca cgcaacgaat gatttgaatg
     9541 cgatccagaa tgtagcagga gctggtatct tgacctttgc agattcagtg attacgggcg
     9601 gaacaacgat cattgcgatg attttgttta ttgattggcg cttaacactg attgcgctga
     9661 ttcctctgcc tttgctggcc gtcacttcac gagtgctggg gtcaaaactc catgatgctt
     9721 ttcgtgattc gcaagaagct ttttctagta tcaatgataa gacacaagaa agtatcactg
     9781 ggatcaaagt cattaaaaca tttggacaag aaaaagaaga tctagcggat tttgaagaaa
     9841 agattgaaga tgcaattatt aaaaacaaac gagtcaattt tttagactcc ttatttgatc
     9901 cattcattac cttgattatc ggtctgtctt atgttgcaac aattatttta ggtggaaagt
     9961 atgtcttgga aggcacgatc tcgatcgggc agatggtctc atttattagt tatatcggta
    10021 tgcttgtttg gccgatgttt gcgattggtc gattgttcaa cgtattggaa agaggaaatg
    10081 cttcttatga tcgcgtcaat gaacttttgc atgaaaagac gcatatcgtg gaacaactgg
    10141 gtgcagtgca gaccccggca aaaggcgagc tgaatgtagc tatttcctct tttaattatc
    10201 cgaaagaaga atcaccagca ttagaacaaa tcaagtttgc gatccaagaa ggagaaacat
    10261 taggtattgt tgggaaaact ggtgcgggaa aaacgacgat catcaaatta ctgatgcgtg
    10321 aatacgatga ttatcaaggg aaaatccaat ttggcggacg ttcgatcaaa gaatattcat
    10381 tgaatgcttt attaggatca atcggctatg tgcctcagga tcacttcttg ttttcgatga
    10441 ccattcgaga taatatccgt ttttctaatc caaaactttc agaagaagaa gtagagcatg
    10501 cggcttcttt agcatttatc aacaatgaaa tcaaggaatt accaaaaggt tatgatacct
    10561 tagttggaga acgtggagtc tctttatctg gcggacagaa acaaaggatt tcgattgctc
    10621 gagcattgat caaaaatcct gaattattaa tattagatga tgcactctct gcagtagatg
    10681 ccaaaacaga agaagctatc ttgcataatt taaaagaaat aagaaaagag aaaacaacta
    10741 tcattgcagc tcaccgttta agtagcgtga tgcatgcaaa agaaatcatt gttgtcgatc
    10801 aagggaaaat cattgaacga ggaacccatc atgagctgct tgcactaggc ggctggtatg
    10861 aacaaatgtg ggagaaacag caattagaag cgaagataga aggaggcgag gcctgatgga
    10921 tgaaaattat caatcggaat ggacaaaatc gattccactt aaagaacaga aaaagatcat
    10981 caaacgactg atgaagttcg ccaaaccttt tagaagaacg ttcatcgttg ctatcttatt
    11041 tgcgtttgct ttgtctgtga tcaatgtcct attgccaagg atcatccaaa cttttatgga
    11101 tgatcatctg gctaaacagt cggctacgac acaagttatt ttgttttttg ctggtatgta
    11161 cttatttgga gtcatagtaa aaagtatcat ttggtttttc caatggtatt tgtattccat
    11221 ggcctcattg aagacctatc agtatattag ggtaaaatta tttgaaaagc tgcatacact
    11281 aggtatgcgt tattttgatc agacaccggc aggatcaacc gtttctcgag tgacgaatga
    11341 cacagaaacg ctatttgaat tttggtacgt ctttttgatg gtattgactg ggatctttgc
    11401 agtcgtttct tcattccttg ccatgtttca aatcaatgga gaaattgcct tgtggacttt
    11461 ggtgtttttg ccgattttag gaatcgtcat ctggtattac cagaaattca gttctcgtat
    11521 ttaccgaaat atgcgggaaa aattaagtca gctgaacacg aaattgaatg aatcgatttc
    11581 cggcatgcgt attatccagc agtttcgcca agaagctcgt ttagctaaag aatttgaaca
    11641 agtgaacaat gagtatttag acacgcgtta tgcgatgatc aaaacgaatt cgattctgtt
    11701 aagtccgatc atcaattttc tatatgcatt ggcgattgcg ttggtgttga ctttgtttgg
    11761 gatcgatgcg ttgcattctc ccgtagaagt tggactgatc tatgcattta cgacctatgt
    11821 ccagggattt tttaatccaa tgtctcaaat gatggacttt ttgagcatct tcacagatgg
    11881 gatggttgct ggtagtcgta tcttgaagat tatggatacg gaagaatatg caccacaaca
    11941 aaaccctgga gcagatcaaa tgattacttc aggtaagatt gagtttcgca atgtaagctt
    12001 ttcttatgat ggagagcaca atgtattaaa caatatctct tttgttgcta atccgggaga
    12061 gaccgttgca ttagtgggac acaccggaag cggaaaaagc tcgatcatca acgtattgat
    12121 gcggttttac gaattttatg aaggtcagat attgattgat gatcatgaca ttcgagaata
    12181 tccaatggaa gagttacgta agaaaatggg gctggtcttg caagatgctt tcttgtttta
    12241 tggggatatc gcaggaaata ttcggatgat gaatccagct atcacagacg aacagatacg
    12301 ccatgcggct gagtttgttc aagcaaacaa attcatcgat gaattaccaa aaggatatca
    12361 ctcaaaagtg atcgaacatg gtgccagcta ttctagtgga caacgacagc tgatcacttt
    12421 tgcgcgaaca atcgtgactg atcctaaaat tctagtattg gatgaagcaa cagcaaatat
    12481 cgatactgaa acagaaggat tgatccaaga aggattggca aaaatgcgtc aaggacggac
    12541 aacgattgcg attgcccatc ggctgtcaac gattcgagat gcagaattga ttttagtttt
    12601 agacaaaggt catatagtag aacgcgggaa tcatgaaaca cttctagcgc aaaatggttt
    12661 gtatgcagat atgtacaaac tgcaaagcag tggtatataa aagatgttgt gacagtagaa
    12721 ttttgaccta agcactagag tgaaaataaa acatagcttc tcatattttt catctgtgag
    12781 agttagtgtc gtagtcacca gatttaaaaa aagattcgac agctagtcag aagtgcctaa
    12841 tagccactaa gaaagcgttg acaaaagcat ttgctttcga taatataaag aaaacgatac
    12901 aacttggata gtgattgcct gatgcaagct aaacctgatg agtctcccat ggtttcatgg
    12961 acggcttttt gggtttttct ttttgttttg gatacagccc tatacatcat ggaaaggagc
    13021 aaaacatgct gattgagaaa gtactgaata acaacgtcgt aataaccagg gatgaaagaa
    13081 atcaggaaat gatcgtgatg ggaaaaggtt tggcattcaa aaagaaagtg ggtgacccga
    13141 tttttgactc atgcatcgac aaaaaatttc agttgacaaa tgacgggctt tcacaacagt
    13201 tccaagaatt ggcacaagcg attcctcttg agtacttaga aatttcttgt gaaatcattg
    13261 aatatacaaa agaggctcta caagtccagt tgaatgattc tgtgtatatt gcattgacgg
    13321 atcatttgta tacggctatc gaacgtcaca aaatgggaat atttgtacca aatgtattgc
    13381 tgtatgatat caaacaacta tttcctaaag agtatgccat cgggaaaaaa acagtgaaga
    13441 aaatctcgga taaataccac atcaagttat cagaagatga agccggattt atcgcattgc
    13501 atttagtcaa tgcacaaggc attcaaagcc agcaagacaa tacctacctg tttactataa
    13561 caatacagga tattctaaat atcgtccgct attactataa gattacattt gatgaagatt
    13621 cagtccattt ttttcgattc gtcacacact tacgtttttt tctgacacgc gtcctagatg
    13681 gagaaaaatc agaagtggga gaagtttctg aggaattgtt tcaggttatt caaaaaaaat
    13741 ataaggatac gtttggttgc gttgagaaaa tagcagactt tttagccgag acgttcaact
    13801 accagatgtc cacggacgaa aaaatgtatt taacgatcca tactgcgcgt ttagtgcatg
    13861 agaatcgttg taaataaaaa aaggaggata aattaacaag aacgtatgaa ataagggtag
    13921 tgacattcag ttggctaaac cttccactaa taaattggag ggaatagaaa tgggaaaata
    13981 ccaagaatta gcgagaaaaa tcgtagaaaa tgtcggcggg aaggaaaata tcaatggttt
    14041 gacacattgt atcacaaggc ttcgtttcaa attaaaaaac gaagaaaaag caaatgatga
    14101 gatcttgaaa aatatggacg gtattgtaac ggtaatgcga gcgggagggc agtatcaagt
    14161 tgtcattggg aatcatgtgc cagtcgtgtt tgaagaggtt ataaaagcag gaaaccttac
    14221 ttttgatgag gctgtttcaa cagaaaaaat gagaccgttt gatatgctta ttgatggaat
    14281 atcgggctgt ttccagccat tccttgctat cctagctgct ggcgggatga ttcgtggact
    14341 gacagcattc cttgtcttct taggcgcgtt tgaccgcggt tctggcacat ttgttatgtt
    14401 tgataatatc ggtgattctg tattccaatt tatgcctgtt attatcggtt taacagctgc
    14461 ccgtaaattt aaagtaaatg aattcgtagg gatgttgctt ggcgcagcat taatgaatcc
    14521 tagcttgtcg ctggaagctt tgagtggtgc agcagaagca ccactaacga ccatattttc
    14581 tggcactatt tttgaagcac ctatttatca aacagtattc ggcattccct ggattgcgcg
    14641 taactacgct tcttctgtca ttcccattat ctttattgtg ttgttagcta gccaaattca
    14701 gaaaccaatt aaaaagttag tacctgagat gatcgcgaat ttcttcgttc cattcttcac
    14761 cgttcttatt acaatgcctt taggtttttt actcgttgga ccagtattca cattcgcaac
    14821 tgatatttta atggctggat ttgaaacact tttagcactt tcgccagtta tttatggagc
    14881 aatcgtaggc ttcttctggc aaattctggt gatgtttggt ctccattggg caatcgtacc
    14941 aatgggattg atgcaatttt ctgtgaatgg ttggcaaaat attatgacac ctgttgcagt
    15001 agtgtctttt ggacaaactg cagcacttac cgcactctac tttaagctta gaaatccaaa
    15061 agacaaagca attgcaatac cagccattgt ttctggtatt gtggggatta cagagccagc
    15121 tatttatggg tttacattac cacgtaaaaa aatatttatt tttacctgtg tcggtggtgc
    15181 gttagggggc gcgtatagtg ggttaatgaa tttaacttct tggaatcaag gtggactagg
    15241 catctttaca attcccaact atattagacc agatggtgat ttaacagatg tgattaatgt
    15301 attgattggg atcgccattg cgatggtggt atcctttacg ctgacttttt tcttttggaa
    15361 ggatgaagca ggtgaaacag atgatataca gaaaaaatca ggtaaagaaa ttgtaaaaac
    15421 accgatacaa ggacaaatcg ctccactaaa tgcagcaaag gatgcagctt ttgcacaagg
    15481 aacactaggt cgaggaatct tgatctaccc tgaaaaagga gaagtacgtg ccccttttga
    15541 cggaacagtc atgaccctgt tcccgacgaa acaggcaatt ggaatggttt ctgaaacagg
    15601 gctggaattg ctgattcatg tcggattgga tactgtgcag ttggaaggga aatattttga
    15661 aagcttagtg cagcaaggat aaagagtgaa acaaggcgat ctgttgctac gcttcgacaa
    15721 aaagaaaatc gaaaaggaag gatacagttt agaaacacct gtgatcgtca ccaattacac
    15781 ggattatttg gatattttgg aaaaccaagg ggagacggtc gggccagacg atgcacttat
    15841 caccgcactg acctgaacgg ttaggtgaag aatggactag gcttaactat tctttttatt
    15901 ccacttcttt attttcaaaa ttgaaaggct ctttcatgat ggctgagctg atagggcgtt
    15961 tatcgaattt ttgaccgaca gaaattagat ggactaaatt ctgttgataa ttttctgcaa
    16021 aaacacaact atataacacg tctagaagat aacaaattga tgtattgatc gtaaaattac
    16081 caatttttga atacagtcgc tcgcgtgtcg taagtcgcaa agcggctgta ctcattgcag
    16141 atagcgttga atcaccgata ctggtcaaag ctaaaattgg aacatgattt tgtttcaata
    16201 tttctgcggt acgaaggatc acattgtttt cacccgtata agaaatcaga atcccgcaca
    16261 tatcactcgt gcaattgtac gcttcataga atgattcacc aaaagtggtg acaatcgtga
    16321 catccatttt gatgcgcttc attttcagca tgaaatcttg agaaatcaaa gtattcgagt
    16381 tactggcgaa aatcacgatt ttttttgctt gaagcattag ttgtttggct ttttgcagtt
    16441 ggtcatgatg aatgagagaa agtgtatcat gaatcgtcat ctgttctaaa gaagccattt
    16501 tatttgcgat cgtcataata ccgtctgtgg tggaaaaagg aagattcgca tctacgtctt
    16561 gaaaattccc ttcaagatag gctgtttcag ctaaaaaagc attacgaaaa ctgacccagc
    16621 catcaaaacc catttttttt gctacacgga tgagtgtaga ggggtggaca taacatcgct
    16681 cagcaatcgc ttggatggtc atttcgccaa gtgctggaga atcgcttaag agaaactgga
    16741 tcacttcatt ttctgaagga gaaaaggaga tttctttgat tttttcacgt aataacatgt
    16801 agataccttc ttcttatttt tttacgtaaa ttataacttt tttgaaaatg atttgta
//
LOCUS       DACCXO010000056        16119 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.057, whole genome shotgun sequence.
ACCESSION   DACCXO010000056 DACCXO010000000
VERSION     DACCXO010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.057"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            41..898
                     /locus_tag="IVY70_001669"
     CDS             41..898
                     /locus_tag="IVY70_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP7958799.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1013..1933
                     /gene="pstC"
                     /locus_tag="IVY70_001670"
     CDS             1013..1933
                     /gene="pstC"
                     /locus_tag="IVY70_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP7958800.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1933..2817
                     /gene="pstA"
                     /locus_tag="IVY70_001671"
     CDS             1933..2817
                     /gene="pstA"
                     /locus_tag="IVY70_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP7958801.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVSEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2832..3638
                     /gene="pstB"
                     /locus_tag="IVY70_001672"
     CDS             2832..3638
                     /gene="pstB"
                     /locus_tag="IVY70_001672"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP7958802.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3654..4412
                     /locus_tag="IVY70_001673"
     CDS             3654..4412
                     /locus_tag="IVY70_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP7958803.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKNKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4425..5102
                     /gene="phoU"
                     /locus_tag="IVY70_001674"
     CDS             4425..5102
                     /gene="phoU"
                     /locus_tag="IVY70_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP7958804.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5339..6370
                     /locus_tag="IVY70_001675"
     CDS             5339..6370
                     /locus_tag="IVY70_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP7958805.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTNQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKHGLFALGSLIINIVLLLLLLLIFTKIEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNLWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            6367..7134
                     /locus_tag="IVY70_001676"
     CDS             6367..7134
                     /locus_tag="IVY70_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP7958806.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTTIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7234..8802
                     /gene="liaX"
                     /locus_tag="IVY70_001677"
     CDS             7234..8802
                     /gene="liaX"
                     /locus_tag="IVY70_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP7958807.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            9102..9419
                     /locus_tag="IVY70_001678"
     CDS             9102..9419
                     /locus_tag="IVY70_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP7958808.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            9419..9769
                     /locus_tag="IVY70_001679"
     CDS             9419..9769
                     /locus_tag="IVY70_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP7958809.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            9936..10874
                     /locus_tag="IVY70_001680"
     CDS             9936..10874
                     /locus_tag="IVY70_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP7958810.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10889..11719
                     /locus_tag="IVY70_001681"
     CDS             10889..11719
                     /locus_tag="IVY70_001681"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP7958811.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11763..12788
                     /locus_tag="IVY70_001682"
     CDS             11763..12788
                     /locus_tag="IVY70_001682"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP7958812.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHCLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPVEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12803..13741
                     /gene="galU"
                     /locus_tag="IVY70_001683"
     CDS             12803..13741
                     /gene="galU"
                     /locus_tag="IVY70_001683"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP7958813.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13866..14306
                     /locus_tag="IVY70_001684"
     CDS             13866..14306
                     /locus_tag="IVY70_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP7958814.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14309..14911
                     /locus_tag="IVY70_001685"
     CDS             14309..14911
                     /locus_tag="IVY70_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP7958815.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(14970..16076)
                     /locus_tag="IVY70_001686"
     CDS             complement(14970..16076)
                     /locus_tag="IVY70_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP7958816.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
BASE COUNT         5219 a         2805 c         3492 g         4603 t
ORIGIN      
        1 tgataaaata aacatacttt aggattaaag gagatttcta atgaaaaaac ttaaattatt
       61 cttaccaata ataggattag ctattctttt atcaggctgt tcaaagtgga ttgatcgagg
      121 agaatccatt acggctgttg gctcttctgc tttacagcct ttagtagaaa cagttgctga
      181 aacttaccag acagaaaatc ctggaaaatt catcaatgta caagggggcg gaagcggaac
      241 tggtttgtct caagtacagt caggagcagt cgacatcgga aactctgatt tgtttgcgga
      301 agaaaaatcg gggatcgatg cctcagcatt agtcgatcat cgagtagcag ttgttgggat
      361 cactcctatt gtgaacaagg atattggcgt aaaagacatc agtatggaaa acctgaaaaa
      421 actatttaca ggcgagatca aaaactggag tgaagtcggt ggaaaagatc agccagtagt
      481 tatattgaac cgtgcttcag gaagcggaac ccgtagcacg tttgaaaaat gggtattgga
      541 cggagaaaca gctgttcagg cacaagaaca agattcaagc ggaatggtgc gccagatcgt
      601 tgccgacaca cccggtgcaa tcagctatgt tgctttttct tatgtttccg atgatgtaga
      661 tacgttgaat gttaacggtg tagaaccgac agatgaaaat gtgacaacga acaaatggac
      721 gatttgggca tatgagcata tgtacacaag agatcatcca aaaaaactga caaaagaatt
      781 tttagactat atcttatctg atgaagtaca agataacatc gttggagaac taggttatat
      841 ccctgtttct catatgaaag tcgaacgtga ttggcaagga aatatcgtta aagagtaatc
      901 tttacacaaa atttacaaac ttagctaaac ttttacatgt tattaaaacc aaattgatgt
      961 tcaatttgta cactgagtga gtatgttaaa cctgattaag ggggagtcat ctgtggaaga
     1021 tgtgaaaaaa gtattattaa caaaatcaaa acgttcaaaa atggaacagc gtggaaaatt
     1081 catcagtttt ttatgtatcg cgctgatcgt gttggttgta ttatctattt tttattttgt
     1141 agctagtaaa ggattggcca ccttttttac tgataagatc aatgtatttg attttctgtt
     1201 tggtacagat tggaatccaa gccaaatcgg agcaaatggg aaaccaatgg taggggcatt
     1261 gcctatgatc acaggttcgt ttattgtgac ctttttatct gctattgttg ccacaccatt
     1321 tgccattggt gcagcagtat tcatgacgga gatctctcct aaaaacggaa cgaagatctt
     1381 acagccggtc atcgagctgc ttgtaggtat tccttctgtc gtttatggtt ttatcggttt
     1441 atcagttatc gtgccttttg tcagaagtat ttttggcggg acagggtttg gtatcttagc
     1501 tggtacattt gtgttgttcg ttatgatctt gccgactgtc acaacgatga ctgttgatgc
     1561 attaaaggca gtaccacggc actatcggga agcttcatta gcgcttggtg ccacaagatg
     1621 gcaaacaatc tacaaagtcg tgctacgagc agccgtgccc gggattttaa cagcagtcgt
     1681 ttttggtatg gctcgtgcgt ttggtgaagc cttagctatc caaatggtca tcggtaatgc
     1741 agcattgatg ccaacaggac ttactactcc tgcttctaca ttgacgtcca tcttaacaat
     1801 gggaatcgga aatacaatca tgggaacagt tgaaaataat gtgttatggt cattagcgct
     1861 gatcttgcta ttgatgtcat tattctttaa tatcgtgatc cgtatgattg ggaagaaagg
     1921 agagctgaaa taatgaacgc aaaacgctca gataaaattg ctacaggtgt tttatatgta
     1981 gtttcgggga tcatcgtatt gattttagca gctttactac tttatatctt agttcgggga
     2041 ataccgcaca tttcatggtc cttcttgacc gaaccttcca aagcctacca agaaggcggt
     2101 ggaatcggga tccagctatt taactcgatt tatttgcttt tgatcacgat gatcatcagt
     2161 tttcctattt ctttaggtgc gggtatctat ctttcagaat atgctggaaa aaactggctg
     2221 acagatgtga ttcgcacgtc gattgaaatc ttgagctcat tgccttctgt cgttgtcggt
     2281 ttattcggtt tcttggtttt cgttattcaa ttccagtatg gtttctcgat tatttctggt
     2341 gcattggctt tgactgtatt caacttgcct cttttgacta gaacagttga ggagtcttta
     2401 caagcggtcc attacacaca acgagaagca ggattggctt taggtttgtc acgctgggaa
     2461 acagtgataa aagtggtggt ttcagaagcg acaccaggga ttttgactgg tgtgatccta
     2521 agttctggaa gaatttttgg tgaagcagct gcattgatct atacggctgg acaaagtgca
     2581 cctgcgttag attttacgaa ctggaatcca ttaagtgttt caagcccgat cagcattttc
     2641 cgtcaagctg aaacattagc tgtccatatt tggaagatca atacagaagg cacgatgcct
     2701 gatggtgttt ctgtttcggc tggcgcttct gccgtgttga tcattgctgt tttactgttc
     2761 aacttcggcg ctcgtgcgat tggcaaacga ttataccgaa aaatgacttc agcctaagga
     2821 gaagaagcaa aatgaaaaaa tataactgga atgaaacaaa tgtcatcacc atgccaaaag
     2881 agaaagaagt agcactatat acggatgatc tacatgtctg gtatggcgac aatgaagcaa
     2941 tcaaaggagt ggatctggaa tttgaaaaaa ataagatcac tgcactgatt ggtccttccg
     3001 gctgcggaaa atctacgtat cttcgttcgt tgaatcgaat gaatgatggg atcgctaata
     3061 cgaaaatcac tgggaaaatc atgtatcaag gcgtagatat taatgcgcct caagtggatg
     3121 tttatgagat gcgcaaacgg attggcatgg tttttcaacg tcctaatccg ttcagcaagt
     3181 cgatttatga caatattaca tttgctttga aacagcacgg cgaaaaaaac aaaaagaaat
     3241 tagatgaaat cgttgaaaca agcttgaaac aagctgcctt gtgggaccaa gtaaaagaca
     3301 gcttggacaa aagtgccttg gctttatctg gcgggcaaca gcaacgttta tgtattgctc
     3361 gtgcgatcgc gatgaaacct gatatcttgc tgctagacga gccggcaagt gctttggacc
     3421 ctatttcgac aggaacggtt gaagaaacac ttgtaaactt gaaagaagac tatacgatca
     3481 tcatcgtgac gcataacatg caacaagcgg ctcgtatcag cgactatact gcctttttct
     3541 atctaggaaa agtaatggaa tatgacaaaa ctaacaagat atttaccaga ccaaaaattc
     3601 aggcaaccga agattatgtg tccggtcatt ttggttaaga cggaggcctc aacatgacaa
     3661 aagaaattat ttcatctaaa gatcttcatc tgtattatgg aaagaaagaa gcactaaaag
     3721 ggatcgatct aagtttcaat caaggggaaa tcactgcgat gatcggtcct tctggttgcg
     3781 ggaaatccac ttatctgcgt tctttgaacc ggatgaatga cctgattccg aatgtaacga
     3841 tcaccgggag tgttttatat aaagacaaag acatctatgg tccaaaaaca gatgttgttg
     3901 aactaagaaa agaaatcggc atggtattcc agcagccaaa cccatttcct ttttctatct
     3961 atgaaaatgt catctatgga ttgaaattaa aaggggaaaa aaataaaaaa gtccttgatc
     4021 aagtagttga agaaagcctg aaggcagcgt ctgtttggga tgatgtgaaa gacaaattgc
     4081 ataaaagtgc tttatcttta tccggtgggc agcagcagcg tgtatgtatc gcacgtgttt
     4141 tggcagttaa tccggaaatc atcttattgg atgaaccaac aagtgcgctt gatcctgttt
     4201 ctactgggaa aatcgaaagt atgctgttag aattaagaga gcggtatacg atgatcatcg
     4261 tgacccataa tatgtctcaa gcttcacgta tttcagataa gacggcattc tttttagatg
     4321 gtcacttgat cgaatacaat gatacgaaga agatattcat gaatccagaa aaacaagaga
     4381 cagaagatta tatttcaggt cgatttggat aaggaggaaa cggaatgcta cgtacacaat
     4441 ttgaagaaga attgctgaat ctccataatc agttctacga aatggggatg atggtgagca
     4501 gcgccgtgca caagtcagtt cgtgcataca tcaaacacga taaaatctta gcccaagaag
     4561 ttatcgaaaa cgatataaat atcaataaca tggaaacacg cttggagaag aaaagttttg
     4621 aaatgatcgc attgcaacag ccagttacaa ctgatttgcg tatgatcatt acggtcatga
     4681 aagctagctc ggatttagaa cgtatggccg atcatgcggt gtctgttgct aaatccacga
     4741 ttcgcgtaaa agggcagacg agaataccgg aaatcgaaaa agagatctcc gatatgtccg
     4801 attatgtgaa aaaaatggtg gacaacgtcc tagttgctta tgtcaaaaca gacgaaaaag
     4861 acgcacgtac gatcgccaat atggatcaaa gagtcaatga ctatttcaat cgaatctata
     4921 atgcaacaat caaaaatatg caggaaaatc ctgagacagt catcagtgga acagactact
     4981 tgaatgttgc cagttatcta gaacggatcg gagactatgt gacaaacatc tgtgaatgga
     5041 tcgtctactt ggctacaggg aaaatcactg agttaaatac gaaccacaat gagattttct
     5101 aaactaaagc ggtgattctg ccgtttgttt aggaaaagag gttgtgaaat cagcggttgt
     5161 gaaaaagctg ttctgcatca agtaataaga acagtcaaac aaaaatagct cctcatattt
     5221 ttcatttggc tggacttatt accgcagatg caagcttttg aacactgttt attcgggaaa
     5281 gggtttgtga caaaatatgt cacaaaccct tttcatcatt tcatagaaag gataaagaat
     5341 gcaaaaaaaa attagcatca tcactatatt ttttatttgt gtgcttactt catgggttat
     5401 tcagcatcaa gctgctttat atgaagtacc tgtcatcgag atcgaaaaag tacgaataaa
     5461 agaggatcat caattgatta caggaagact gatgaacacg aatcaaaaag gagaatcgat
     5521 ttatttatcg gttccttatc aaaaaaatca aatagaaaca gcaaatataa aagcgagaca
     5581 aatgtatcta gtccaaatgc atggagatga atggcagcta gtagcaaaaa aaaatgatgg
     5641 ctggcttttc ttcttcacaa gtatcttcat tggcgccatg ttgcttattg gcggaaaaca
     5701 tggtctattt gctttaggaa gtctcatcat caatatcgtt ttacttttac ttttgttgct
     5761 catctttact aaaatagaag ggatttctct attaaatata tctatcctgt tcgtcgttat
     5821 agggatgatt attagcatat tggctttaga tggatggaat agaagcagta taatcaaaat
     5881 ttcagctgca gttacgagtg tttttttagc attttttatt tgtcttttga caatgaatct
     5941 ttggaaagac caaggactgc gctatgagga actgaattat ctgacacgac cgtatcgttc
     6001 tgtttttctt tctagcgtac tgattggaac agctggagga acactcgata ccgttatcac
     6061 agtcattgcg acattagaag aattgactaa gcgacaacca gaaataacag cgaaacagtt
     6121 atggctttcg ggaaggcaag tgggtcgaga tgtgattggc tcgatgacca atatcctttt
     6181 gttcgtctat atcagtgggt cagttccatc gcttctttta tatttaggaa atcaatggtc
     6241 gttcaaagaa acgatagaac agcatttatc attggaattt ttacgcgtca ttgctggcag
     6301 cttaggaata gtattatcga tccctatctc ggtcctcttt tttctgactg taaggaggct
     6361 gaaacgatga atgtacttat tatacttgtt gggattttag caatcagcgt gctacttatt
     6421 ggcggtacgc aaggtatgta tattctttta ggcttattca tcaatttagg gatctttttc
     6481 ttgttgttat tcggttatca tcagaagtgg ccgatactgg cattgtcggt cataggattt
     6541 ttattgattg ctgtggttat cttatttttt atcaacggtt ataatctaaa aatgcgtgca
     6601 gcttttgtga gtatactgat ctttttattt tgtttcctac tattgcttcc tatcactgat
     6661 tttttagcaa tccaaggttt tacaacgata gaactagaag aattatctgg cttagataag
     6721 actttagcga ttgattttag attacttacg agatcgttgc ttctgattag tcttagtggt
     6781 gctgttttag atgcgagtgt agcaatttct agtgggacat tcgaagtata tcaggcaaat
     6841 ccgcaattat ctttttacca attgcgccat tctagtttcg ctattgctaa aaaaatattg
     6901 gcgtcaacag taatgacact tctttttgca ttcatgggaa gttctttagc gttgatcctc
     6961 tggtttattg atttagacat tccttttcag caagtgatca atgaaaaatc atttgttcta
     7021 gaatatacga tggctattct taccacattg tccgcactac ttgttcttcc tttaacctgc
     7081 gtgactacag catatctctt tacaaaaaag aaagaacaac tcaagatgga gtaaaagtta
     7141 caacttaagt cctatgacat ttttactgaa tcagtgcata atatggatgt aaggaaaagc
     7201 ttgcaaacaa ttttgcttaa ggaggcaatt tccatgaaag aaagagaacg tattttagat
     7261 ttagtgaaaa aaggaatcct ttcgactgaa gaaggcttag atttattaga aagcatggca
     7321 actgaaaaag acgaaaagca aatcaaaaaa gaagcagaca aggtaactgc tagtcataaa
     7381 gaaaaagatc aggcaagtca attgatcgat aaacttgaaa acggcgaaga agaaatttct
     7441 gagccagttg atccaaagga aagagaaaga caagatcaag aaaacttaga aaagatctta
     7501 gatgaactag caactgaagc gaataaaact tcggcgcgtc tagatgaagt aaatgctcaa
     7561 ttcgctgaca ataaggcagc acgcaatgag aaacaggaag cattgatgca gctgaataca
     7621 aaagaagaat taggcgagtt gacagaagaa gaattagcac agcgtcagac gttagaagca
     7681 gaaatcaaag agttagaagc tagtggcgat acactgttag aagaacaatt gaaattagaa
     7741 gcagaattaa aagatatccg taagaatcaa tggtctgaga aaaaagaaac gtttactgat
     7801 aagtttgaat tgccagatga ttggaaagat caagcaacag atacactgaa tcaagtaggc
     7861 gaaaaaatgt cagaagctgg atcacagtta ggaaaattct tgaagaaaac attccaaact
     7921 gtttcagaaa cagtgaatga caacatggaa tggaaagatg tcagcttgcg tgttcctggg
     7981 attgcgacaa cgaaatttga gcatgaattt tactatgaag ctcctgcagc aagtatcctt
     8041 gatatcaagg cagcaaacgg aaacgtgacg ttaaaaacgt gggacagcaa cgatgtgaaa
     8101 gttgaagcta agatcaaact ttacggaaaa atgggtgctg aaccatttga agcattctct
     8161 gaacggagcc agattgaggt taatgaagat catatctcct tccaaattcc aaataaacgt
     8221 gtgcgtgcag accttgtttt ctacttacct aaacgagtat acgaccacgc agctattaaa
     8281 cttctaaatg gcaacatcat gattgaaaca ttggaagcta aagatattta tacaaaatct
     8341 acgaatggta acatcatcgt ggaccaatta actgcaacga tgctggaagt agaaggtgta
     8401 aacggcaata tcgatattcg aaacgggaac atcttagatt ctattatcga aacagtaaat
     8461 ggtacagtga catttggtgc gacagcagaa aacctttctg tgtctttggt aaatggtgat
     8521 gtacgtctta cgattaaaga agataatttg aaaaaagtag aagcaagttc agtgaacgga
     8581 aatgtcaaag tcgctttacc agatggcatc ggtttggaag gacatgctaa aacaagtctt
     8641 ggaagtatca acagccgtct gtccaattac gaagtagttc gtgagaaaaa agaacggaca
     8701 aaccaaatgt tgcaattccg tcgtgtttca gatggtgaaa tcgcgcaagt gcaactttca
     8761 acgacaacag gaagtatcta tttaaaagat acagataaat aaagaggttg tgaaagagat
     8821 gttcagcctt gagcaataag aagcgatttt gtaaaatagc ttcccctatt ttacaaaatt
     8881 gtgccttatt gccgataggc tatctcttga acaccgttta ccaagagatt atgaacttgc
     8941 caattatcgc cgagtcacaa ggaataattt caaaaaaagc tcgtcacgtt ttatgaatat
     9001 ggtaatttgt gaccgagaat tttgcaaaaa gacaccgatt aagaagaagg ttgtcacaac
     9061 gattttcttc atttaaaata gtagaacaga ggtgagacat gatgaaaaaa aagttgacaa
     9121 aatccaataa aaatgttgtt ttgacaggga ctttagcagg aataggagaa tacatcggga
     9181 ttgatccgac tgttatccgt gtgctttatg tattcctgag cttagttgct tttggaagtc
     9241 caatcatttt atacatttta ttagctttga ttatcccatc tgcgcgttct gcacgaacaa
     9301 actatggaca taacaatgca tattatgaaa aaaacaatta tcgtgaacaa actcgtgaac
     9361 aaacaaaaaa acgcaaagaa gcggaaaaaa tcgatgatga cgaatggagt gacttttaat
     9421 gtcgtacttt caacgtattt tagttaatac gttaacattc atatcattgg ctgttctttt
     9481 tcctgcgatg gtccatgtga gcagtatttg gacggccgtt cttgccagct tcgtattgtc
     9541 tgtgttgaat atgtttgtca aaccgatttt gacgatactg tccctgccct ttacattagt
     9601 cacattggga ttgtttacgt ttgttatcaa tgcagcgatt cttaaaatga cctccttttt
     9661 tgtgggagca gaacatttta gtttttcttc attcggtgca gcgttgttag tagctatctt
     9721 gatgtcgctt gtcaatgtaa ttgtcagcga acataacagt agaaaataaa agaaagctgt
     9781 gccgaactat cggcgcagtt tttttattta gaatcaggaa tgcgttttct attacattgg
     9841 taattaaata aaaacgtttt ttttaattgg tagtactttt ggctagacaa tgataaaatg
     9901 atagagaaca ctctaataga aatgaggtag catgaatgtc agaagttgta aaagttagcc
     9961 aattagtgaa aaaattatct ttggaaatcg tgacaggcga cgagaaaagc ttagaccgtg
    10021 tcatcgtaac tggggacatc tcacgcccag gactagagtt gactggttat tttaattatt
    10081 attcccataa tcgtttacag ctatttggca gtaaagaaat cagttttgcg gaacggatga
    10141 tcccagaaga gcgtttgatc atcatgagaa gattatgtag tgaagatatg cccgcattta
    10201 ttatttcaag aggattacca gctccagaag aaatgatcca ggcagcaaat gaaaatggga
    10261 tagctgtact gcgttctccc atctcaactt ctcgcttatt aggagaatta tccagttatt
    10321 tagatggtcg ccttgcccct cgaacaagtg tccacggtgt tttagtggat gtttacggat
    10381 taggtgtact gattcaaggt gacagtggga taggaaaaag tgaaacagcg ttggagttga
    10441 tcaaacgcgg tcatcgattg atcgcagacg atcgagtaga tgtttatcag caagatgaat
    10501 taacggtgat cggagaaccg cctaaaatcc tggaacatct gattgaaatc cgaggaatcg
    10561 ggatcattga tgtaatgaat ctttttggtg cgagtgcggt acgtggctct atgcaagtcc
    10621 agttggctgt ttatctggaa gcatgggcaa aagataagaa atacgaccgt ctgggctctg
    10681 aagacacgac cgttgaaatc gcggaagtag gcattccaca aataaaaatc cctgtcaaaa
    10741 caggacgaaa cgttgcaatc atcattgaag tagccgcgat gaatttccgt gcacgtacaa
    10801 tgggctttga cgcaacgaaa acatttgaag aaagattaag tcgcttgatt gaagaaaact
    10861 caggcaacga ctagcaagga gtgacaatat gttagcacaa atcaatcgag ttgcttttga
    10921 gcttgtaggg attcctatct actggtatgc attgatcatc gtatcaggta tcattatcgc
    10981 catgtggctt agcagcagag aggctgttcg ggtaggatta aaagcagatg atgtgacaga
    11041 ttttatgttg tggggattgc ctatctccat tattggcgct cgcttatatt atgttttatt
    11101 tgatcttccc cagtatatag ctgatccaat ccaaatattc aatatccgtt caggaggctt
    11161 agctatttat ggcggactga ttgcaggcgc gatcacgctg tattttttta ctaaatatca
    11221 ttttatttct ttgtggactt ttttagatat cgctgcacca agtgtattat tagcacaagc
    11281 aatcggtcgt tggggaaatt tcatgaacca cgaagcatat ggaccagaca cgacgcgaag
    11341 cttcttagaa agtctgcatt taccgcaatt catcattgat aatatgtata tcgaagggat
    11401 ctatcgtcaa ccaacatttc tttatgaatc actttggagt ttgattggat ttattttgct
    11461 tttgttttta cgaaagaaac cacacctttt gaaaaaaggt gaagtccttt tatgttatct
    11521 aatgtggtat agtttcgggc gtttctttat tgaaggatta cgtacagaca gtctttactt
    11581 tttaggtgtg atccgtattt ctcaattatt atctgctgtg ttatttgtcg gtacattgat
    11641 tctatttatt tggcgcagaa aaaaaggaaa tttaccggat tatgaccgct cattaggaaa
    11701 aaatcaacaa attatctgat ttttagagcg ttaaaaatga aaaaaatttc aggagtggta
    11761 aaatgaatta tagagtagcg gttttaggac caggctcttg gggtactgct ttggcacaag
    11821 tcctcgccga aaacggacat gacgttcgga tttggggaca tcgtgcttca caagtagaag
    11881 aaatcaacac ccaacacacg aatcaacgtt atttaccaga tcattgtcta cccgattcca
    11941 tccaagcatt caccaatatg gaagatgcag taaaagacgc agatgcggtg ctattcgtca
    12001 ttccaacgaa agcaatacgt tctgttgcaa aagaactagt gcctaaattg aatacaaagc
    12061 cagtgatcat ccatgcaagt aaaggattag aacaggggac acataaaagg atctctgata
    12121 tcttgttaga ggaaatccca gtagaaaaaa gaaaagctgt tgttgttctt tctggaccaa
    12181 gccatgcaga agaagtagcg aaacatgaca tcactacgat cacagctgca agtacagatg
    12241 caaaagcagc aaaatatgta caagagctgt tcatgaataa ttattttcgg atctatacca
    12301 atcctgatgt gatcggtgta gagatggggg cagctttgaa aaatattatt gctattggtg
    12361 ctggcgctat tcacggctta gggtttggcg ataatgcaaa ggcagcaatc atgacgcgcg
    12421 gattagctga gatcagccgt ttaggtgtag caatgggtgc caacccgctg acctttatcg
    12481 gattaagcgg agtaggcgat ctagtcgtta cttgcacaag tgttcattct aggaactggc
    12541 gcgcaggcaa cttgttagga caagggcagc ccctagaaga ggtgctagcc aatatgggaa
    12601 tggtcgttga aggtgtgaat actacccaag cagcgatgga actagcccaa ataatggaaa
    12661 tcgaaatgcc catcactcaa acaatctatg aagtgctgta tgaacaaaaa gaaatcaaaa
    12721 cagccgcaaa agaaatcatg ttacgtgatg ggaaaaaaga aaatgaattc tcattaacaa
    12781 ttttttgaag gagcgaatgt atatgaaagt caaaaaagca gttattccag cagcaggatt
    12841 aggaacaaga tttttaccag caacaaaagc tatggcaaag gaaatgttgc caattgttga
    12901 taaaccaaca atccagttca tcgttgaaga agcgttgaaa tcaggtatcg aagacatctt
    12961 gatcgttaca ggaaaagcta aacgaccaat cgaagatcac tttgattcaa atattgaatt
    13021 agaaacaaac ctaagtgaaa aaggaaagac cgaattattg aaattagtag aagaaacgac
    13081 agatgtcaac ttgcatttca tccgtcaatc tcatccaaag ggtttaggac atgccgttct
    13141 tcaagcaaga gcctttattg gaaatgaacc atttgtcgtt atgctaggcg atgatataat
    13201 ggaagacgaa gtaccattga cacagcaatt aatcgatgat tacgaacgta ctcatgcttc
    13261 tacgatcgcc gttatgcaag ttcctcatga tgaaacttca aaatacggaa ttatcaatcc
    13321 aggagaagta ttggaagatg ggttatacaa tgtcaaaaac tttgttgaaa aaccagatcc
    13381 aagcaaagca ccaagtgatt tagctattat tggacgttac ttattgactc cagaaatttt
    13441 tgatgtattg tccgagcaac aaccaggagc cggaaatgaa atccaactaa ctgacgcaat
    13501 cgatacattg aataaaacac aacgcgtatt tgcccgtaaa ttcacaggta aacgttacga
    13561 tgtcggtgac aaatttggtt ttatgaaaac aagtattgaa tatggtctaa cacatccaga
    13621 agtaggtgca cctttacgtg agtacatcct gtctttaggt aaaacattag aagcagaagg
    13681 aagcaagatc gatcataatc caaaaggaat aacagaaatc aaagaagaaa agaaaaaata
    13741 accctttttt ctaacgagct tcagctgcta attgaataaa aagtagttga agctcgtttt
    13801 tatttgttta aaaaacacga aaatgctatc cttatagaaa agagagacaa gaagggagtt
    13861 tgatcatgtc aggaggagaa attgcaggat tgatcgcagc aatcgccttt gcgatacttg
    13921 tcgtttttat cgttcgtgtg ttgttgcaag tttctaaaac tttagaaaaa gtagatcaga
    13981 ctgtagcaga agcaaatacg acgatcgaaa tcgtcacaaa agacgtagat cttttgtcta
    14041 gacaagtaga aggcttactg gtcaaaagta atgaactgct cttggatatc aatggaaaag
    14101 tcgcaacgat cgatccattg ttcacagcag ttgctgattt aagtgaaagt gtatcagaac
    14161 ttaatcattc tagtaaaaac atcgcgacga aagtcggcgg aatcggtaaa acgacagcta
    14221 aagcgacggt tgccggaaaa gtcggtggga cagcgatgaa attttttaaa aacaaaaaac
    14281 atactgaaac gacaggagga aaataaaaat ggctaaaaaa ggtggatttt tattaggtgc
    14341 agtagtcgga ggcacagccg cagcaatcgc cgcgttattg ttagcaccaa aatctggtaa
    14401 agaacttcgg gatgaattag ctgcccaagc ggatgatttg atggatatgg cttcagatta
    14461 cacagatatg gcgaaagaaa aatcgactga gttaacaggt attgcaaaag aaaaagcgca
    14521 agatctttct caacaagcag gtgatctagc aggtagtgtc aaacaaaaag taagtgaatc
    14581 agcagatgaa gcaggaagtg aatctgacga tatcttgtta aatcttaaag aacaatcagc
    14641 tgacttatct aatcgtttca aaaaaacggc agaagatatg aaaaaacctg cagatgaatt
    14701 aggaagtatt gctaaagata cttcagaaga tatcatgatt gatgtaaaag acgctgctgg
    14761 taaagcagga gatactgtca atgaaggaat gtcagaagca aaaccagttg cagatgactc
    14821 aaaagaaatg atgaaaatgg caaaagaaga tgcgaaagaa gctaaaaaag aaataaaaga
    14881 tacaatgaca gattcatcta caaatcacta aaatcacaaa tttatagaat aaaaaggtgg
    14941 gagcggacta gctctcacct tttttactct tatttttcta aaacaagtaa ttcttttggt
    15001 gtagtcgtga atggcacaca gccatcgctt gtcacatata cacagtcttc gatacgtacc
    15061 cctacttttt caggaatata gattcctggt tcaagagaga agcacatccc ttcttcgatt
    15121 actaagtcat ttccttctac aagggaaggg aattcgtgga cggttgttcc gatcccgtga
    15181 cctaaacggt gattgaaata ttcgccgtag ccatagctgt tgatcacgtt tcgtgcgatt
    15241 tgatcaagtt cacctgctgt cacacctgga cggacggctt cttgtgcggc aagctgtgct
    15301 tcaagagtga tgttgtagat tttttcttgg aagtcggttg gtttttgata agcaattgta
    15361 cgagtagcat cactgcaata gccattccaa acaacgccaa gatcgaataa cacaagttca
    15421 ttcggtgaaa tcttcgtatt tcctggtgta ccatgagggc ttgcagcatt tgctcctgca
    15481 aggactaatg tgtcgaaaga catttgtgaa acaccttgtt ttttcaattg gtattcaatt
    15541 tctgcaatga tcgcttgttc agtcacgcct tctttcactg ctttaaagcc aatctcaaat
    15601 gcaacatcgg cccatttacc agcttcgatc agtttgtctt tttcctctag agttttgatc
    15661 agttgtagac gttggatgat ggaagttaga tcagtgtaag aatccgttcc cgtcaagata
    15721 tcagagatag catcgaagcg ctcgacagtc aaggcgttct tttcaatccc tacattgcca
    15781 ggctgaccat agcgagcacg aagttctttt ccgatgatct cccaagggtt ttcgctgtct
    15841 aaatagccac ggacatcata cgcccaacta ctgttttgag catcttcaac ttccaaagca
    15901 ggagtgaaaa ggaaaggatc ttgttcaaga gggataaaaa gagctagtac acgctcatga
    15961 ggatcacttt cgtatccgga aaagtaagcg atgtggccag ggtcgctgat ataagccaat
    16021 tcgacttggt gttgtgccat ccattgacgt agttcattga tttttacgtt attcatttgg
    16081 caacctactt tctaaattaa ttttgtattc aataattgt
//
LOCUS       DACCXO010000057        16056 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.058, whole genome shotgun sequence.
ACCESSION   DACCXO010000057 DACCXO010000000
VERSION     DACCXO010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.058"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            198..866
                     /locus_tag="IVY70_001687"
     CDS             198..866
                     /locus_tag="IVY70_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP7958817.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1066..1296)
                     /locus_tag="IVY70_001688"
     CDS             complement(1066..1296)
                     /locus_tag="IVY70_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958818.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1423..2805)
                     /locus_tag="IVY70_001689"
     CDS             complement(1423..2805)
                     /locus_tag="IVY70_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP7958819.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2961..4148)
                     /locus_tag="IVY70_001690"
     CDS             complement(2961..4148)
                     /locus_tag="IVY70_001690"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP7958820.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4155..5186)
                     /locus_tag="IVY70_001691"
     CDS             complement(4155..5186)
                     /locus_tag="IVY70_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP7958821.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5202..6656)
                     /locus_tag="IVY70_001692"
     CDS             complement(5202..6656)
                     /locus_tag="IVY70_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7958822.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            6831..7736
                     /locus_tag="IVY70_001693"
     CDS             6831..7736
                     /locus_tag="IVY70_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7958823.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7827..8504
                     /locus_tag="IVY70_001694"
     CDS             7827..8504
                     /locus_tag="IVY70_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP7958824.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8785..9681
                     /locus_tag="IVY70_001695"
     CDS             8785..9681
                     /locus_tag="IVY70_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7958825.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9736..11106)
                     /locus_tag="IVY70_001696"
     CDS             complement(9736..11106)
                     /locus_tag="IVY70_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7958826.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11344..12090
                     /locus_tag="IVY70_001697"
     CDS             11344..12090
                     /locus_tag="IVY70_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP7958827.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12087..12926
                     /locus_tag="IVY70_001698"
     CDS             12087..12926
                     /locus_tag="IVY70_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP7958828.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12927..13877
                     /locus_tag="IVY70_001699"
     CDS             12927..13877
                     /locus_tag="IVY70_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP7958829.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14018..14770
                     /locus_tag="IVY70_001700"
     CDS             14018..14770
                     /locus_tag="IVY70_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958830.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14789..15940
                     /gene="queG"
                     /locus_tag="IVY70_001701"
     CDS             14789..15940
                     /gene="queG"
                     /locus_tag="IVY70_001701"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP7958831.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5207 a         2995 c         3229 g         4625 t
ORIGIN      
        1 gttgtgtaaa aatatcattt tagagcataa aaaaaaggag tggatttctc cactccaaaa
       61 aagtctaact tttgggggtc actacagtgt cacagtcctt tttttagtaa aaggatacat
      121 ttcttccttg tatattccat gcgtgttcgt gagaaagcat atataattta attaacagat
      181 agttaggggg agaaaaaatg attttttcaa ttacaatgaa actggtgttc gggttgatcg
      241 gtttattatt agtggtacgt ttgcttggga agaaatccat gtcagaaatc acaccgtttg
      301 atttagtcta tacgcttgtt ttaggtggga tcttagaaga atcaacctat gatgacaatg
      361 tccatgtagg gcatgtccta tttgccattg cgctttgggc agtaatgatt tatggtatcg
      421 aacgtatcgt ccaaaaaaat gaaaaggtca atcgttgggt caaaggcgag ccatctgttc
      481 taatcaaaga cggcgtcatc aacatgacag agttgacaaa taaccatatc gagatggagc
      541 agttgcgagc aatcttaaga cagcaagaat gcttttttct tgaaaatgcc aaacatgtta
      601 tcctagagaa tgccggtcaa atgagtgtat tgaaaaaatc agatgaagac aaagctttat
      661 cgatcctgct tgtagatgaa gggcaaattc agcataaagt gcttcagtcc aatcaattga
      721 ctgaagcatg gcttatggag aacttgaaga aagaaggcta tgcagatgta aaacagctga
      781 tttatgttga atggtcagaa gaaaaagggc tctacatact aaataaagaa gacgtcatgc
      841 atgaatctta ccgaatagat ggatagatag acagaggttg tgaaatcagc gccttggctt
      901 gttactcaaa actaagcgac tttttcacaa cctctgatat ctaatatacg ttgttccaga
      961 agttgcttct tcgaaaaata agccaaaatt ccacaaaaat ttaagaagca attttcgaaa
     1021 aatcctttct tattttttga agttgatcac ttctgtcaca acctctcatg tcacaaacca
     1081 tttttttgtt tcctttaact caatataccg tacgtcttcc cacaatatcg gctgatggtc
     1141 aatcataaag taatcttcat aggcttcacc aatgaagtgc ccttcgacct catccaagag
     1201 tcttccaaat tcatctttta aattgagctg aatgattact gctttacgag ataaaaaggc
     1261 ttctgtcagt atactatcga tttcttgcaa tgtcatttgt tgtttaggaa gaggctgttt
     1321 gcgtgcttct tcttcatttt tttgataccg ttgactagtt cttccatcgc aaaagcagtc
     1381 ccccatttta atccaaatgg ccgatctcga tactcgttat agtcaatgaa tttttttgct
     1441 gtccgtctca tcttttccac cttctaatcc actcatacca cctgcatgcc cgccaacgag
     1501 agaactacgt gcgatggcgc gtcctccttg caagagacta tgcgcatgga tgattgattg
     1561 aaagccgtat ttttttctaa tcgtatcgac aacaaaatca actttcgctt catttatctg
     1621 cttttcagga tcttcaaaaa ggttaagctg taaacctgtc gcgtatacga ggtcggaaca
     1681 gttcacgcct acacgccgaa tatcctggta gtggtaatgc tggtggaaaa tggtcaataa
     1741 agcatctgct agatctctgc tagcatttgt cgcttcgatt ttcatttgct ggtgaaaccc
     1801 ggtcttgcca tttggatcaa gatagcctaa agagtatcct acccataagc tgactacttg
     1861 tgtttttgct gcagatcgtc tcaagcgagt gccgacttgg tcggccattt ctttgataac
     1921 gatctcgatt tcttctcgtc ttgtataatc tctatctagg atttgactat ttccgatact
     1981 ttttgatttt ggtgtatatt cttgtcccaa aaaacttcga tcgatgcccc aagcatgggc
     2041 ataaagctga gtaccgatga cccctagctt gcttttcaag tgataataat ccgcgtgagc
     2101 taaatcataa acagaatgta tacccatttg tttcagacgt tttgctgttc gtttgccgat
     2161 tccccaaaag tcagtgatat ctgagatttt ccataacttt tctggtacat cttcataacg
     2221 ccattcagca aacatttgct ttccgtcttt tgagccattg tctaaggcta gtttagcaag
     2281 tagcgggtta tcgccaattc ctatagtcgt gtagattccg gtctgctcaa acacatcttt
     2341 atggatcagt ttagccatgt cgtacgcatt tgttttacca aagagtgcca tgctatttgt
     2401 tacatcaagg aagctttcat cgacggaata gacatgatgg ttcgcttcgt ctgcatattt
     2461 tttatagata gaattgattt ctctgttctt ttccatataa taggccatcc ttggggcaac
     2521 aatatataaa tcacttggat aggggaaggg aagatctctg gcacggctga tattggtgat
     2581 gccgtaagct ttttttgctg caggactgga agccaagatt aatccgctac cccgtgtttc
     2641 tgtcgaatcg gacggatagg acatgactac taatttagtt gtaaggggat caagatcctt
     2701 ttcaacgcac tctacggagg cgtaaaaaga tttgcagtca atacataaaa tatctcttga
     2761 aggttctttt tcataaacaa ataccggatt ttgattcgcc cacataacaa tcacctcttc
     2821 aacacaatta gaacacacgt tctctttccg tgtcaataaa aaacagcaat tttcaaatac
     2881 gagcaaaagg ctcacatttg gaaattgctg agaggttatt tttatctatt tgtttttagc
     2941 tgagatcgtt gattttttta tcaaacatga acagcttctg gatcgatctt tgttgtccca
     3001 tttccaatgt gtgcaacata aaattccgga gcatagccga caactttcat gtattcatca
     3061 tagacattgt ttttgaaggc aggtatattt tcttctttga caagagcaat cgcacagcca
     3121 ccaaatccag ctcctgtcat tcgtgcacct aagacacctt cttgtttttg agcagcatct
     3181 actagtgtat ctaattcgat cccagttact tcataatcat agcgaagaga gtggtgagaa
     3241 tcatttaaca gttgaccaaa tgcttctaag ttcccagctt ctaattcagc ttttgccttc
     3301 aaagtccgct gattttcaga aacggcatgg cgagctctgc gtatcaatgt atcatcgccg
     3361 atttggtcaa tagttgattc aaattcttct tcatctaagt ctcctaaaga agagataggc
     3421 aaagtttttt gcaaacgtgc caaggcttct tcgcattcgc tacggcgttc attgtatttt
     3481 gaatctgcca attcccgccg cttattcgta ttcatgatga cgattgcata accatctagt
     3541 ttcactggaa ccatttcgta tttcagcgta tttgtatcta ataagattgc atgatctagc
     3601 tcaccgaagc cgatggcaaa ctgatccatg atacctgaat tgacaccgat gaattcattt
     3661 tctacttttt tacctgtttg gactagttcc aagcgatctg tttttaaatc aaacagatct
     3721 tctaatacaa cacctactaa taattctaat gaagcgctac tggaaagtcc tgcaccatta
     3781 gggattgttc cttccactaa taactcaaac ccatgatcga ttgagtgtcc agcttctttt
     3841 aatttaagga ccattccttt tacgtaattt gcccagtttg cttttttatc atattctagc
     3901 tcatctaaag taaaactaat gacaccgata tcgtcaaagt ttgtggaata aactaatatt
     3961 ttattatcgt ctcgaggtgc tgctacacct tgcgttccat aagtgatgga agcggggaag
     4021 acatggcctc cattgtaatc tgtgtgttca ccaatcaaat tgatgcgtcc tggtgagaag
     4081 tattgtgtag tttttttagg tccgaatgtt tcggcaaatt ttgtgtttaa gcttcttttg
     4141 atttccatga tgagttattc ctttcttgtt ttgattttga attctgttac tgtttcaaat
     4201 actgatccgg cttttaaatg aatcgaacca aatgctgaga agcgctctgc acctggagct
     4261 gtttgagtct caaatgtgat gccaccgtga tgtgccagtt ttcggttgcg catttctggt
     4321 gtttctgtgc cgaaatttgc agtgaaaata accacactag aagcatctgt agcaatatct
     4381 acttggattc ttttatcggg gctagtcaag cgagcggctt ctttacccaa gccggtttct
     4441 tttaagaaaa atggatgatc gattccgtca acaagatttt tttgatccaa atcactagca
     4501 aaaacggacg ctaatttttt aggtatttga aaatcgaaag cacttcctgc agcattttct
     4561 tttaccccta taggaatact atctgttcgc aaaggagcat aagcttcgct attgagccaa
     4621 agctcatgtt gatcgattga ctgagaagca tctcccgtca agttgaagta aacatgattt
     4681 gtcggattga ataaagtatc ttggtcactg gtgccgcgag aggtcactcg ccagatatta
     4741 tcttttgtca aagtatagcg gatttccaca tccattgtac ctggaaaacc atgttctccg
     4801 tcgggacttg tagtagtaaa aatgacagaa gcttcatttt cgccattcaa aatgacataa
     4861 gaccattttt ttaattcaaa tccaggagca ccaccatgca gattatgtcc agtttttgga
     4921 tctgtggcta attggtaagt ttttccattg agagaaaagc ggccattttc gattctccct
     4981 gcggtgcgac cgatagaagc accgatatac ggatcttttt cgatatattc ttcagcggtg
     5041 tcaaatccca aaactaattc tcgatcattt gattttaaac tgacgattct agcacctaaa
     5101 tcagagatag aaagtgctag cttgtttttg ttctccaagg ttatcaagtg ataaccttgg
     5161 ccaaaatctt tttctgtaat cttcattttc aagatccttt cttaatggga aggatctact
     5221 gcatcgtgaa gttcatcttc cattgtatca gctggagcca atttgttttc caatttctca
     5281 acgatttgtg catgtttttt ctctgtcaat gtaatcttcc aagcaaaaat aatagcggag
     5341 aaaatcatca aagcagcagg agcatagaat gcataagctt tgaaagtcgt tacgccatga
     5401 gcagtgatat cagcagcagt agcattacca gtcatcccag aagcgatagc gacaaaccct
     5461 acgatcccgt tagacaaagc accagcgatt ttatctagta aagggcgaat tgatagagta
     5521 acagcttcgt tacggatacc atttttccat tgaccatatt caacagagtc tgtgatcgtc
     5581 atcaaagcgg ataagaaaat caattgttga gggaagtaga acaatactag cccgatgata
     5641 acgattggca aacttgaacc tgcgatcgta aagaaaaagt aagaagaaat catcattgcg
     5701 atccctccaa ggtaaacgta gcgacgagag atgacgcgtg taagcattgg gaatagtgga
     5761 acagctacta aaccaagtaa ggcagcaact agaccaacga tccaaaattc agttggttgg
     5821 ccaagtacat atttgaagaa gtagaataat acggcagttg ttgctgcatt ggcaatcgca
     5881 taagcgatat aagcaagggc aagccacatc aactgatcat tttgagcaac tgctttgaac
     5941 acatctttga tcccagcttt ttcattttgc tgacggatcg cagtatcgac ttcttttgtg
     6001 cccaaggcag tgataatcgc agtaccgcct gaaataacag cagcaatcac accgaaccag
     6061 aaccagcctg aagcgccttc aatatgttta tgagtaacaa gataggtaaa gaaagtaacg
     6121 atagggacaa cgactagagt agtaccattt gcacctagag aagagccgaa acgagcaaac
     6181 gtagcaaatt tttcacgttc ctttgtttcc gtactcaaag ctggaatcat tgaccaaaaa
     6241 gcgatatcct taaatgaata aaaactatcc aataagatga aaacgattgc aaataatatg
     6301 ataaataaag tcgtattatg agtagctaag ccaaagaaat tggtaaagat tgctaccaag
     6361 agtacggaac taacaacgcc gccgataact aaccatggtt tgaatttacc gtattttgta
     6421 cgagtattat cgatgatgcc accaatcaaa ggatcaaaaa taatttcaac taagcggata
     6481 cctacaacta agcttgtaac tgtagcaatc atttttgcat tcgttgcctt atctgttcct
     6541 gcgaacatct ggcttgtcac gaataccata aaataagtgc ttaatgtaac atagaatgtg
     6601 tcgtgtccaa aagcgcctaa agcgtaggac acacgttgga tcattttgct tttcataatc
     6661 tgtattcctc tcctgttcaa tatataatac taagtaaacc ggttacaacg aataacaaag
     6721 atcttttaat gacttaaact taacaagaaa tcgcttcctt tttaatatat ttctgtgact
     6781 tttatttaac tttttgttgc attatactaa aggagtaagg agggatagac gtggagcaat
     6841 cgattttttt aaaaaaggaa catcaacaag tagaaaaaga attgtatttt gatttttgtg
     6901 gtttctctaa aacgatgccg tttcattctt ttggtcctgc tattcgcaat gattacatcc
     6961 tgcatgtcgt gatggaaggc aaaggatctt atcatgtgaa aaatcaacaa ttccaattga
     7021 aaaaaggtga tctgttcttg attcgtccag gagactcgac gttttactta tcagatggag
     7081 aagatccgtg gatgtattgc tggctttcat ttggtgggag tgctgcagaa gaaatcattc
     7141 ggcagtcgct tttcagagaa gaccaataca cgatggtctc ttctgacata caaaaatata
     7201 tcgacattat tttagaatgc atgaaagata cggaagaaac cttggaaaat gaattacagt
     7261 taacagcatt aacctaccgt tttttatcag tccttttaaa agatggcgga aaggtcagcg
     7321 gtggtgtaca aaaggttcat tcctctttag ctgttgaaac agtcaaatac atcgaggcac
     7381 attatgctga aaaattgtct gttgaggaaa tcgcccagca tttagcggtc aatcggagcc
     7441 atttgtcgcg agtattcaaa aatcatatgg gtaccagtat caaggaatat ttgatcgggg
     7501 tacggatcaa tcgaggggcg tttcttcttt ctctgacgga tgaatccgtg gaaagtattg
     7561 cctatcaagt agggttcaat agtctagtcg tatttagccg aatgttcaaa aagagtaccg
     7621 gagaaacggc tacgaactat cgtaaacgta tgaaaaacga aaattttaag aatattgctt
     7681 tggatacctt gaaaaaacag ttggaaaaac aaaccgcagt ttcttggaca acctgattag
     7741 tctggttaaa atgacaagca tcggcaaaag aaacttgtgg cggttatgct acactgtaaa
     7801 gcgaagacta aaaaggagga aaagggatgc gtacacttat catcattagc catccaacga
     7861 tcgaagaatc tttaaatcag caatttttca aagaagcttc tgctggttta ccagctactt
     7921 ggcatcattt ggaatcaagt tatccagacg gaaaaataga tatagagact gaactacagc
     7981 tcttggccca gcatgatcgg atcattttcc agtttccgtt ttactggtat agttctcctg
     8041 ctcatttaaa aatttggcaa gataatgtct tggaacgagc agcacatgta ttaaaaggga
     8101 aagaactagg aatcgtattg acaacaggtg tggcggaaaa agaataccaa gctggaggaa
     8161 aagaagaata tacgatttcc gaatttttga ggccgtatca gcgaatcgca aataaattcc
     8221 atatgaccta tcttccgcca ttcgttttag cacaatttat gtatttatcc caggaaaaac
     8281 gttgggagaa gctaattgct tatcagcagt atctctcatt ggaaggcaaa ccatcgctga
     8341 ctcagcggat tgactggttt attcaaagag tacaggaaaa tcagaaaatg caggaagaag
     8401 aatctgaaaa acaagcatat atcgtagaag cactgactga ggcaaaagag cagatcgaag
     8461 acttgacctt tacactgcaa gaaatgaaag gaacttcatt atgacaaatg attggctgat
     8521 ccaacaatta gatgaattaa gcgaaaaatc agaatatcaa gaccgtgcgc tcttatatga
     8581 aataaagaaa ctgatggctg agatagacga acgaacaatg cagctgcaag gagaaatcga
     8641 cggacaatct tggaatcatc tcaactggtg aaattttctt ataaataaca aagataaaaa
     8701 tgtgcacagc gacaattatt tgctacaata agaatatgaa gcgtttgata acgctattat
     8761 acataaaaat tggtggtgaa aaaaatgtat gatgaaaatg agtggggaga aaatgttcgg
     8821 atgacccgaa gaagacagca aaaacaggca gctcgtaagc ggatgatcat gattggggca
     8881 gcagttgccg tgtttttgat catagcagga ggagttgcgg gtctatattt tcatggaaaa
     8941 aattcgtcag aagcgaaaag tacgaccata acggaaacaa cgacaaagaa aacagagacg
     9001 aaacaaacaa gtgagtcatc aactaaaaca aaagagacac aaacttctga agaaaaaaca
     9061 gcaaatggtt tgccagagca acttcaagca atcgtaaatg attattcgaa aaagatcgaa
     9121 gaaaagacgc ctgcattgat tgaagaatac cagacagaaa ttcagggaaa tcaagaggga
     9181 atagctggtt tatcagcagt tgctaaccaa aaagcacgtg agcttcaagc aatctccgac
     9241 gaagggatca gaaaattacg tgcagcgtat caggcagcag aaaataaaga cggtgtagac
     9301 ttagatactt tgatcaatca attatcggct aattatacga atcatgtcgc taagatttcc
     9361 gatatttatt tgcagactag cgcctctctt caagcagagt caacaagcac acaagacaca
     9421 acatcttctg attctgagac agcagagtcg gtagaagctt ctgaaatgca agctcgaaca
     9481 acacaagaca caacagattc ctctgaaact aataccaaca gcgcgacttc acaggctgcg
     9541 acaaccgtag ttttggaagg agaaggtcca aatcaaatag ccgaacgaac tggcgtacca
     9601 gtagaaacga tcttgtcgct gaatgggatg agcatggaga attatttcct gactcccggt
     9661 gagacgctaa aattgaattg ataaaagcaa taaaaaagaa cgaagcttcc actaaggtga
     9721 agcttcgttc tttttttatt ttccggttct tttttcgcgt acaaagtaat ggatcgtcat
     9781 gtaaagcaag aaaacgagtc caataatcaa tgagattctc gtttcaggat tgataaacat
     9841 gaaaatcaat acgatagcta aaaaagcaag tgtcacataa ttcgtataag gagatagtgg
     9901 cattttaaaa ggatgatcat gcattttatc tgcgtgcttc tttttgaagc gaatctcgct
     9961 gatcaaaatc actacccaag gaaccatacc aggcaaaata cttgagctgt atacgaaaac
    10021 gaaaatattt tctgactggt tgatgaacat tggtagaaga taatttaaga tcagaccaat
    10081 taagattcca cctgagattg ctacgacagg ataaaaaggc gcaccgtgac gagatagttt
    10141 cagaaatttt ttagacatct ttccatttat ggcaagtgta tagaccatac gacttgcgct
    10201 gaaaatccct gaattgcaac ctgataatgc cgctgtaatc acgacaaaat taatcagtcc
    10261 tgcagcaaat gtaatgccga ctttagaaaa agtttgtaca aaaggtgaac caatctcact
    10321 caattgattc cacggataga tactcacaat gataaaaata gcaccaatat aaaaaatcaa
    10381 gattcgaccg atagtggact ggacagcttc aatgatcgtc tttttaggat tctctgcttc
    10441 accagcagtc attccgatca gttcgattcc ttgataggaa gcaacaacga tcgataatgc
    10501 aaagaaaaat cctttgacgc ctccagtgaa gaatccacca tttttccaaa gatttgaaat
    10561 ccctactgcc tgaccatgat taccaaatcc aaataagatt accccaagac ctgcaatgat
    10621 catcaaaatg atcgttatga ctttgatgat cgaaaaccaa aattctaatt cgccaaacgc
    10681 cttgacagaa ataagatttg ccagcataag aaacaagacg acaatgattc cgggaataat
    10741 tgccggcaaa tcaggccacc aataattcat ataagatcct acagcaatca cttcacttat
    10801 tcctacgaca atatattgaa aaatattact ccaagcggtc aagtagccag ccacaggatg
    10861 gatgtactcg ctcgcgtaat ttgcaaaaga accagtcgaa ggatcaacgt aaagcatttc
    10921 gcctaaagca cgcataatca aatacaaaaa gaatcctgca atcgcatagg ctcccatcac
    10981 tgagggccct gtccaacgta tggttgagga ggcacccata aacagaccga caccgatcgt
    11041 tccacctagt gcgatcattt ggatatgacg ggcactaaga cttcgagtta acttttcttc
    11101 ttccatacaa aaactccttt cgttgttgag tcaacaagaa aaaataatca aacgtttcaa
    11161 cacatgtttt tcattattag gttagcctta ttgaactctt gatgtgattc tacagaaaat
    11221 tttcataaaa tgcgaggaaa attcgaaaaa aactttttca tcattctact tgaaaatgct
    11281 ttccatttta gttaaactat atttaaataa aaagttaggt aaacctaaaa aggagtgtaa
    11341 gatttggaac gtacggccat tgtaattcaa gacctttcag ccgcttatca agggaaaact
    11401 gttttgaaaa ctatcaactt gacgataaat ccacaaaaaa ttaccggtat tatcggacca
    11461 aacggtgcag gaaaatcaac atttatgaaa tctttgctag agttgatacc agctactact
    11521 ggtaaagtca ccttttctgg acagtcggta aactcagtcc gtaaaaaaat agcctacgtg
    11581 gaacagcgaa gtgaattgga tctttccttt ccaattgatg tattaggcgt tgtactttta
    11641 ggaacatatc catctttacg aattggacaa agacctggga aaaaagaaaa agaacgtgca
    11701 agacaagctt tgaaaaaagt agggatggaa gaatatgcca aaagacagat cagcgaacta
    11761 tcgggtggac agcttcagag agtttttatt gcaagagctc tagcccaagg agcagaatgg
    11821 atctttttag atgaaccatt cgtagggatt gatgcgttaa gtgaacgaaa gatctttgac
    11881 atcttgcagg aattgaagaa ttcaggaaaa acgattttga tcgtccatca tgatcttcat
    11941 aaagtagaag aatatttcga tgaggttatt cttttaaata aacagctgat cgcttccggt
    12001 ccagtacaag aagtctttac atcgaagaac cttcaattgg cctatggtga gatcatcaga
    12061 catttagtaa aaggaggaga gaaaaaatga tccaatcctt tattgatgga ttaatgaatt
    12121 atcaattcct gcaatacgct ttggtcactt ccatgcttgt aggacttgct tctggagtca
    12181 tcggttcttt tattatttta cgagggatgt ctttgatggg agacgctatc tctcacgcag
    12241 ttcttccagg tgtagccatt tcttatatgt ttggttttag ttacatcttt ggagccactg
    12301 cttttgggat gctaacagca gcagtgattg gtttcgtgac tcagcggagc cgattgaaaa
    12361 atgatacagc gattggaatt gtctttagct ccttttttgc attagggatt atcttgattt
    12421 catatgccca gagtgcgaca gatttgtatc atattttatt tgggaatgtc ttagctgtta
    12481 gggaaagcga tttgttgctg acagcactcg ttagtggaat cgtcttgata tttgtctttt
    12541 tcttttataa agaattgaag atcacatcat tcgatccgac aatggcaaaa gcttatgggc
    12601 tgaacacatc gctgatccat tatcttttga tgttcttttt gacattagtg gctgtagtca
    12661 gtttacagac agtaggaaca atcttggtga ttgccatgtt gatcacacca gccgccacgg
    12721 cttacttgct aacgaaccat ttactgaaaa tgatcattac agctgcagga atcggtatgc
    12781 taagtgcagt tgtcggtgtg tttttcagtt atagttacaa ttggccatca ggagctacga
    12841 tcgtgttagc atgtaccgca ttttttatcc ttgctttttt attttctcca acaaaaggaa
    12901 ttttatttca gaaaggaagc agatagatga aaacaaaaaa atcactcttt ttaatcttgg
    12961 cagttagttt cttggtatta gccggttgcg gaaagcaggc ttctgaccaa gcggatgaag
    13021 gaagtaaaga aaagttgtca gtcgtggcta ccaattcgat cttggcggac atggcaaaag
    13081 aggtcggaac agatcaaata gatatccaca gtatcgtacc agtcggaaca gatccgcatg
    13141 aatatgaagt actaccagaa gacatcaaaa aggcaagcga tgcagatgtt attttataca
    13201 acggtttgaa tcttgaaaca ggtaacagct ggttcgataa cttgatggaa acggctaaaa
    13261 aagaagaagg aaaagattat tttgcagtta gcaaaaatgt agaacctctt tatttaacta
    13321 gcggtgaaga acatacaaaa gcagatcccc atgcttggct agacctatct aacggaataa
    13381 aatatgtgga ggaaatcgca cgtatattct ctgaaaaaga tgcagaaaat gcgacactct
    13441 ataaaaaaaa tgcagaagca tatgtggaaa aactaaaaga attagataca caagcgaagg
    13501 aaagttttgc ttctatcgaa gagaacaaaa aattattagt aacaagtgaa ggggcattca
    13561 agtatttttc acgagcatat gatttgccag cagcttatat atgggagatc aatacagaaa
    13621 gtcaaggtac gcctgatcaa atgaaagcga tcattgatca gataagagca aaagaagtac
    13681 cggttttatt cgtggaaacc agtgtagact caagaagcat ggaacgggta gcaaaagaaa
    13741 cgggtttgaa aatctatgat aaactgttca ctgattccat agcaaaagaa ggagaacaag
    13801 gagattctta ctatcagatg atgaaatgga atattgaaac gatccatgaa ggtttgtcac
    13861 aaacaaaaga aagctgaaaa gtgcaaggtc tagcttgagg agcagacctt gcatttttta
    13921 taagagatat gaaaaaaaat tatgctgttt ttcgtcgtac agataccttt tttgaagaaa
    13981 ttccgttata gttagagtaa ggaaggaagg tgtggctatg aattttacac gtaaagataa
    14041 atatacgatt aaagaagaag tctttcgatt gatcagccag caggaaaata ccgacaagga
    14101 tcaagacctt caatttcttg aagcagaatt agctaaatta ttcatagcaa atcaaaatga
    14161 gacaacagaa ccagatgaag agctgtacat aggcaaatgg ttgtttgatg aatttcagcc
    14221 tttagatgaa aataccggta tcggttattt tgtgttcctt tctccagtcg gcttcacacc
    14281 tggcgtatta gttaaacaaa acgacgaaat aaccgtcaaa gcatggcctg cattattttc
    14341 agtcatcaaa gcagaaggag taatcaaaaa gatccagtcg gcctattata aaagtcaatt
    14401 tattgccaac agcaaattaa tgaatatgtt ttataatcgt ctagtagaaa ttgctaggcc
    14461 tgggaaaatc ccgcaaacat tcacgtacac acagcaaaaa aatctctatg aagaagtatt
    14521 gtattcccgc gatctgtctg agttttcgcc atgggaagag gtaaggctcc atgaactgtt
    14581 aagtacaaaa ccagtttcat tgaatttttt taatgcatta gataacaaaa gaaaattaat
    14641 ttcaacaaat tattgggaag tatttgctgc aagaaaaaaa tggatggaag aaaagaagat
    14701 tgccgatcag cagatccagt ttgtccagct gaaaaaagaa aaactatatt atgggttata
    14761 cagccgttaa cagcaagcga ggaagaccat gacattaaaa gaagaaatca tacaagaaag
    14821 caaacgatta ggaattgaca aaatcgggtt caccacggcg gaaccttttg attcattaaa
    14881 agaatcgttg gaagaacaaa aagcagctgg ccatacttct ggctttgaac atccaaatat
    14941 cgatgagcgt ctttatccag aaatgacatt tgatcagccg aaatcgatca tttccattgc
    15001 acttgcgtat cctactagga tccatgaaga agtgcctagg gatgaaaaac gcgggcagtt
    15061 tgctcgtgct tcttggggga tcgattacca tgatatactc agagaccgtt tgaaccgatt
    15121 gatcgaattc atccaaacaa gagcagaaaa atggcaaaaa gaagaggaat ggcgtctagc
    15181 tccgcaagta gatactggag aactgatcga tgtcgctgta gcacagcgag ctggtttagg
    15241 atttattgga cgaaacggat tgttgatcac tgaagagttc ggttcatttg tctatcttgg
    15301 tgagatcgtt actaatattg tatttgagcc tgataaacca ggagaatttg gttgcggtga
    15361 ctgtatgcgc tgcgtgactg cttgtccgac aaaagcattg cttggtgacg gaagaatgaa
    15421 tgcccagcga tgtttgtctt atcaaactca gacaaaaggg atgatgccgg aagaatatcg
    15481 taaaaaaatg cgaaatgtca tttatggatg tgatatctgc cagttagtct gcccttacaa
    15541 tcaaggaaaa gattttcatt ttcatgaaga gatggaacca aaagtagacg aaatatatcc
    15601 aaaattaaag ccaatgcttt cgatgtctaa taaagacttc aaacagcaat ttgggcattt
    15661 agctggttca tggcgtggga agaaaccttt gcagagaaac gcattgattg ctttagctaa
    15721 tctaggtgat cgaacggcat tgcctgagat ttcagaatgt ttaacagatg tccgccctgt
    15781 catcagagga acagcagctt gggctattgg acgtctagga acaaaagaac cggaaaaatg
    15841 gcttgatgta ttagatgaaa tattagctaa agaaacagaa gaagtcgtta taaacgaatt
    15901 ggaacatgcc cagaaattat tgcggagaaa gttaaaataa aaaaatagtt aacattattc
    15961 aaagtttttg tccagtaatt gttgtaacaa ttactggttt ttttgtattt tttacatttt
    16021 tttaagagaa tttgataaaa atcaaaaatt tttttg
//
LOCUS       DACCXO010000058        15889 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.059, whole genome shotgun sequence.
ACCESSION   DACCXO010000058 DACCXO010000000
VERSION     DACCXO010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.059"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(17..1744)
                     /locus_tag="IVY70_001702"
     CDS             complement(17..1744)
                     /locus_tag="IVY70_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7958832.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1737..2930)
                     /locus_tag="IVY70_001703"
     CDS             complement(1737..2930)
                     /locus_tag="IVY70_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7958833.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3117..3761)
                     /locus_tag="IVY70_001704"
     CDS             complement(3117..3761)
                     /locus_tag="IVY70_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7958834.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(3855..3989)
                     /locus_tag="IVY70_001705"
     CDS             complement(3855..3989)
                     /locus_tag="IVY70_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP7958835.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(3991..5925)
                     /gene="feoB"
                     /locus_tag="IVY70_001706"
     CDS             complement(3991..5925)
                     /gene="feoB"
                     /locus_tag="IVY70_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP7958836.1"
                     /translation="MIARDYLVQEQPELIINIIDATNLERNLYLTTQLLDLDIPMIIG
                     LNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAKKRPSIPVP
                     CTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQKEIEQLIQ
                     LTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATNRWLALPIF
                     AFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQELILNGII
                     AGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSFIPMLIATG
                     CGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSWVAPSAYFL
                     GMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKSFVKKAGTI
                     IFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGTVAAITGLV
                     AKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAPCFAAIGAI
                     HREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILLMIGLVYVL
                     FRQPKSKNQFYTILSLEGEE"
     gene            complement(5922..6122)
                     /locus_tag="IVY70_001707"
     CDS             complement(5922..6122)
                     /locus_tag="IVY70_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HAP7958837.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     IRTFIFRTYPASILCLLIHQKK"
     gene            complement(6119..6592)
                     /locus_tag="IVY70_001708"
     CDS             complement(6119..6592)
                     /locus_tag="IVY70_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP7958838.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            6993..7217
                     /gene="nrdH"
                     /locus_tag="IVY70_001709"
     CDS             6993..7217
                     /gene="nrdH"
                     /locus_tag="IVY70_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP7958839.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7376..9535
                     /gene="nrdE"
                     /locus_tag="IVY70_001710"
     CDS             7376..9535
                     /gene="nrdE"
                     /locus_tag="IVY70_001710"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP7958840.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9585..10550
                     /gene="nrdF"
                     /locus_tag="IVY70_001711"
     CDS             9585..10550
                     /gene="nrdF"
                     /locus_tag="IVY70_001711"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP7958841.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10639..11778)
                     /locus_tag="IVY70_001712"
     CDS             complement(10639..11778)
                     /locus_tag="IVY70_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7958842.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12087..12800)
                     /locus_tag="IVY70_001713"
     CDS             complement(12087..12800)
                     /locus_tag="IVY70_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP7958843.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13054..13755)
                     /gene="nagB"
                     /locus_tag="IVY70_001714"
     CDS             complement(13054..13755)
                     /gene="nagB"
                     /locus_tag="IVY70_001714"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP7958844.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            13989..15521
                     /locus_tag="IVY70_001715"
     CDS             13989..15521
                     /locus_tag="IVY70_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7958845.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15712..>15889)
                     /locus_tag="IVY70_001716"
     CDS             complement(15712..>15889)
                     /locus_tag="IVY70_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7958846.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         4918 a         3293 c         2791 g         4887 t
ORIGIN      
        1 tttcgtgtct gtctttttat tttttccagc tttcgactaa ttcttggttc tctttgatcc
       61 agtcttttgc tgcttgctgt ggatcttttc cattgttgat ttccaacata actttttcca
      121 tatctttttc tgaccaatgg aaattgtcta atactttgta agcttctggc tggtcttctt
      181 tcaatccttt tcgaaccatc gtatggatct gctcttcgct tcccattccg ttttcaggat
      241 cttctaagta tttcagatca tatttggcaa acatccaatg aggtgtccat cctgtaacta
      301 caattggttc gtgttttttg atcgcttgac ctagtgcaac ggtcattgct cctgaagaag
      361 atgtctctac tttccagtct ttcaagttgt catatttttg gatcgtgttt tcagcagccg
      421 taactacacc agctcctggc tcgattccta tgattttctt acctgcctga tcggttaact
      481 catcgatcga atttacatcc atgtaggctg gcactgctaa accaactttt gctcctgtca
      541 aattggcacc taggtcttcg acttgatctt tatattgtgc atattgtgaa ccatgagttt
      601 taggtagcca agctgaaacc atggcatctg actcgccatt tgaaacagat ttccacatga
      661 ttgaattatc taatggcgtc atcgttacat catagcccat ttgtttcaat acttcaccga
      721 ctacatttgt tgaagcaact tctgtatccc attcaacata agataagttg atacgttttg
      781 tttctttggc agatgaaaaa ctccctatac caatcgcacc taagatcact actactacag
      841 caccaatgat cagtccttga cgttttttac ttttctgtgt tgttgctcct gcagcttttt
      901 tattgtttgg ctggttcaaa tgctgtgtaa aacggtcaat gatgattgct aagataacta
      961 atgcaacccc gttgacaaat ccattcccga cttgtgctct ttgcaatgct gataatacgc
     1021 cgcgtcctaa gccgggtgca ccgatcatag aggcaataac gaccattgaa agtgacagca
     1081 ttgttgtttg attgatccct gccatgatcg tacttttagc taaaggcaat tctagtttga
     1141 atagtttttg acgacttgtg ctcccgaatg aatcagaagc ttctactaat tctttaggaa
     1201 cttgtcgaat ccctaaattc gtaaaacgaa cggttggtgg cagagcaaag atgactgagg
     1261 caaatacccc tggaaccatc ccgataccga aaaatgcaac tgctggaatc aaataaacga
     1321 atcctggcat tgtctgcata aagtcaagga tcggtttgat aatactttgg gctttactgc
     1381 tttttgccat caagatccct aatggtaccc caatgatgat cgagatcaca ctagataaaa
     1441 gaacaagtgt gacagtattc atcaaatcat tccatagacc ttggttataa ataaaccata
     1501 gaccgattaa tgtgaacaac gtaagtcctg gacgtttctt agaaataaag aaagctgcta
     1561 ccgtcaataa caagataaac aaaggagctg gaatcactaa cagtaaattt gtaatccccg
     1621 acatcactgc ctgtccaatc gtttgtaaaa atgaaaatag gccagaaaaa gttgtcgtaa
     1681 accattctgt gatattttct acccagtttg ccactggtaa ttgatagttc aatagattat
     1741 tcatgttcac tcacctcgct tgtttctgct aatgcttcaa tcacactacc tcggatgacg
     1801 acacctttta attttccatc ctctacaact gcaagtggag tcggtgagtc ataaataata
     1861 ttgaaaatat cacttaccac catgtctttc ccaatcaccg tcacattttc atctaggaag
     1921 tcgatcaatg gacgtttttg cttcctagct tctaacgctt gatctgcagt gatgatccct
     1981 ttcaggtgtc tctttctgtc aacagccatc agcatgctga cttcttcatt acgcatcctt
     2041 gtcaatgcaa cgtttggtcc atcagattcg atattcgtcg tcaatgcagg taccatgatg
     2101 ttttgagccg taagcacttt ggaacgatcg acttcttcca caaattcacg tacataatca
     2161 tttgctggat tggtcaatat ttcttcacct gttccgatct gcatgatttc gccgtctttc
     2221 atcaaggcga ttctgtcccc aatccgtaaa gcttcattca aatcatgggt aatgaaaata
     2281 atcgtttttt gaacattggc ttgcaggtcc agtaattcgt cttgcatttc tcgccggatc
     2341 agcggatcaa gagccgagaa cgcttcgtcc atcagcaaga tctcaggatc atttgctaaa
     2401 gctctggcta ggccaacccg ttgttgcatc ccaccagata actgactagg atattggtct
     2461 ttaaatgaaa gcaaactaga attttctaat gctttttctg ctttttcttg tcgttcttct
     2521 ttaggtacac cacggacttc taacccgtat tctgtatttt ccaagatcgt tcgatgtgga
     2581 aacagaccga agttttggaa aaccatattg attttatgac gacggacttc tcgcaattct
     2641 tctttgctta acttcgatac atcttctcca tcaatataga tactgcccga agttggctca
     2701 atcagacgat tcaatagacg gatcaatgtg gatttcccac tcccggaaag ccccatgatc
     2761 acgaagattt ctccttcttt tacgtcaaaa ttcacgtcat acacaccaac cgttgcaccg
     2821 gttttcttta aaatctccgt tttcggctgg tgatcattca tcatatctaa agcggcttgt
     2881 gtctttttac caaatatctt tgttaaatgt tttacttcta cttttaccaa acaaattctc
     2941 cttttttatg taaattcatt tttctttctg aaaaaagtga ccacactatg ttaacatcat
     3001 gtagtcactt tgtcaaaaat gaaagaaatt tgcttgtcca aactgaaaaa aacttggcta
     3061 aatctttgta aataaaaggt ttagccaagt taatattttt ttaatatttc acacaatcat
     3121 tctgcaagct gatgctccgg gtaaaaaaag tttgtcatac gctgtagtgt atattcattt
     3181 ccaataaaat gaacgatgtc ccctgccgta aatttagcat aggggccagg tgaaacaagc
     3241 agttcttcat tatgagaaat tgcgataact gtggcgccag tcgcctgcca gatattcaac
     3301 tcgctaattg aacggtccag atgcattgct ttttctgata aagataattc aaacggaagt
     3361 aaaggattca tcttatgtat tcgcttcgtt tgattgacca gctgatccaa taattgagaa
     3421 aaatgattca gctcatcttg ttgcttctcc acacttgcca tgatttctgc ttttgtatct
     3481 tggatggatt gtacatcttg atattgcaat aggaaatctt ttgctttttc cttcgaagca
     3541 acaaatgccc cacttccatg ccgtacttcc atgattccaa gatctaccag cacattgata
     3601 gccttgcggg tagtctcagg agaagcattg tacgtactgg caagtgttga gcgggcatgg
     3661 atcttctcac ccactttata tcggttttca acgatacgtt cggctaaatc aacagcaatc
     3721 tgctggtagc gcgggcggac gatttttttc tttccagaca ttttctttcc tctttctttt
     3781 tttactttcc ctcatcttac tatgctctag caaaataagc aaaaaagggg agaatagcct
     3841 cttgcaacga ctgatcatcg cttgatctgt tcctttttta ctgggcaagc tgtttgacag
     3901 tcatcacaat tcttaccgga ttttaaacga gtatagacaa ttgcgcctgc acttccaaag
     3961 attaacacac ttaataagaa tgtcgccatc ctattcctct ccttctaatg acaatatagt
     4021 ataaaactgg ttcttggatt ttggctgacg gaacaaaaca taaacgagtc ctatcatcag
     4081 taaaatagca agatacgtcc ctaacgctac ctgtccaccc tccaataagt gtcccaactg
     4141 ataaatcaca aaactaacta aataagctaa accgcattga tagcctatgg cgatccaagt
     4201 ccatttcttg gcattcattt cccgatggat agccccaatt gccgcaaaac aaggcgcaca
     4261 aagtaaattg aaggctaaca atgaataagc tgctgcggga gtatatgtgt gttgtagcac
     4321 ttgccagatt tctctgccat tttcggatac ttccgcatga ccaaacaaaa taccaaatgt
     4381 gccaataata ttttctttag cgaccaatcc tgtaatcgct gcgactgttc cttgccagtt
     4441 tccccaacca agcggctgaa aaacaactgc caggattcgc ccgaaaaagg ctaagatact
     4501 ttggtcttca gaaacaggtt gagcataaaa attataagaa gagctgaacc agatcacgat
     4561 actagaaaca aagataatcg ttcctgcttt tttaacaaaa gacttgctgc ggtcataggt
     4621 ttgtcttagg accgctgacc attgaggaaa atgatatgcg ggcaattcca tgataaaagg
     4681 agctgtatct ccagcaaaca aacgtgtttt tttcaagctg attccagaga aaataattgc
     4741 agtcatacca agaaaatagg cagaaggagc tacccaagat tgatgaggaa aaaaggcccc
     4801 tgctactaag gcaataatcg gcaactttgc agaacaaggc atgaaggttg tgaccatgat
     4861 cgtcaaacga cgatcttgct cgttttcaat cgtgcgactg gccatgacgc ctggcactcc
     4921 acatcctgta gcaatcagca taggaatgaa ggattttcct gaaagaccga atttcctgaa
     4981 aagtcgatcc atgacaaaag cgatcctcgc catatatccg cagtcttcca gaaagctcaa
     5041 gcataaaaac aatataatca attgcggtag aaatcctaac actgctccca cgcctgcgat
     5101 gatgccgttc aagatcagct cctgcatcca tcccgctact tgccattcag ctaacaatct
     5161 gccaacatgt tctggtaacc atgtaccaaa caatgtgtca ttgatccaat ctgtccccat
     5221 tgttcctact gtttgaatag ccagataata gatcagccac atcacaaatg cgaagatcgg
     5281 caaggctaac caacgattcg ttgcaatctg atcgattttg tcactcatcg ataattggaa
     5341 agaatcattt gacacaacac agagtgtaca tagttgggta ataaattcat aacgttcatt
     5401 gaccagaatc gtctcactat cgtcgtccaa tagtttctct gttaactgga tcagttgctc
     5461 gatctctttt tgctgagaaa ctgctatctc gtaatcctct cttaccttct gatctcgttc
     5521 aaaatatttc attgcatacc agcgtttctt gccgctttgt tctcctagta catcttcaat
     5581 ttcatgtaaa gctgcttcta aacgtggatc atatgtacag ggaacaggaa tagaaggccg
     5641 cttttttgca gactcctgaa tcttttcgat tagtttttcg attccttgct ttttcaatac
     5701 gctgattggt aaaactggta caccaagacc ataggaaagc ttttctagat tgattttttt
     5761 tccttctttt ttgatgacat ccatcatatt cagaccaatg atcataggaa tgtctaaatc
     5821 caataactgc gtagttaaat aaagatttcg ttctaaattg gtagcatcaa taatgttgat
     5881 gatcagttca ggctgttcct gaaccaaata atctcttgca atcacttctt ctggtgtata
     5941 aggagacaaa gaatagatgc cgggtaggtc ctgaatatga atgttcggat ttttttcaac
     6001 gatccttctt ttttttctac tgttacgccc ggccaattcc ctacatattg attcgaacca
     6061 gtcaagagat tgaacaagct agttttcccg ctgtttggat ttccgactaa cgcgatattc
     6121 attctttttc ctccctttgt atcaagatta gttcggcctc tgactttcgt aacgtcagtt
     6181 cataccctcg cactgtcagt tcgattggat ctcccagagg ggctagtttt cggataacta
     6241 tttttacatt tcgcgtcaat cccatatcca ttaaccgtcg cttcactgcg ccttgtccgt
     6301 ggattgctac gacgatccca gtctctcctg cttttaattg atcaagagaa gtccagcttt
     6361 cagaggaagc agtcttttta ttgacaaaga tttgacctag tatttcccga cttactgcca
     6421 ctcgatgact ttttaccagt aaaatggcat tgttgcctga taaattgacg acgacgattt
     6481 tacttccggc aacgatcccg atattttgca aatggcgttt tgttttttct tctgtttcga
     6541 ttcgttcgac ttgataaacg aaattctgct ttgcctgcgc taaggtctgc atattgattc
     6601 ctctcttgtg aaataaatca tgtattaaaa tcattcatta tatgttctaa ttttcttacg
     6661 tgttacgcaa ataaatgaga atcattctca catagaatac tctcggtaat tgagaatgtc
     6721 aattatactt gtgatattat gagcgaatct ttagtattga tagataaaaa tgagaaaaaa
     6781 atatttcttc atagttttcc acaatttttt ctctattaaa tttgctattt tcccacacga
     6841 tacttgtttt cagctctttt ttcaaaaaca caaaacttag tattccacag tttccacaac
     6901 acaatatgta gtgtttgttg tttgactttt ttactaagag ttttatacta tctattatag
     6961 ataataagaa tgtgttagag gaggcactca ccatgaacgt aaaaatcttt tctaaaaata
     7021 attgtatgca atgtaagatg gtcaaacggt ttttaacaga aaatcaaatt gcttttgaag
     7081 aagtcaatat cgatgaacag cctgaagcag tagactggtt gaaagcacaa ggattccaaa
     7141 gtgttcctgt cattacttct gatgcagaaa cagttatcgg attccgtcct gatcagttgc
     7201 gtaaacttgc cagctaacat gactaaaaga atcaaaacgt gatacatgga tttgaagggc
     7261 ttgagacagc accagttttt gtcgcaaact tccaaatcct tttttaaagc ttaaaaacaa
     7321 tgcaatgatt tgcaatcaag catcatcaaa tacgaaatga aggttgggcg tacgtatgag
     7381 tcttaaaaat ttacaagatg tcagttattt caagctgaat aatgaaatca atcggccagt
     7441 caatggtcaa atccctttga ataaggataa agaagcttta gttgcctttt ttgaggaaaa
     7501 tgtcaaacca aatacgatga cttttccatc tattatggaa aaaatcaatt acttgatcga
     7561 tcaagaatat atagaagcag aattcattca attgtattca ccatcattta ttgaaaagct
     7621 atatcaattt ttggacgagc aacatttttc tttcaaatct tttatggctg cttacaaatt
     7681 ttattctcaa tatgcgttga aaaccaacga cggttccgct tatctggaaa cttatgaaga
     7741 tcgagtagcc ttcaatgcgc tttactttgc gaacggggat gaagaattag cacttgctct
     7801 tgcagacgaa atgatccatc aacgatatca gccagctaca ccttctttct tgaatgctgg
     7861 acgtaaacgt cggggcgaat tggtgtcttg tttcttgact caagtcactg acgatatgaa
     7921 ctctatcggg cgctcaatca actctgcttt gcaattgtcc cgtattggtg gcggcgtagg
     7981 gattacttta tcaaatcttc gtgaagccgg cgctcctatc aaaggatatg agggtgctgc
     8041 aagtggtgtc gttccagtca tgaaattatt cgaagatagt ttcagctatt ctaatcaatt
     8101 aggtcagcgt caaggagctg gcgtggttta tttgaacgtt ttccatccag acattattgc
     8161 tttcctttca gcaaaaaaag aaaatgcaga tgaaaagatt cgcgtaaaaa ctctttctct
     8221 tggcgtgatc gtacctgata aattttatga attgacacgt aaaaacgaag atatgtattt
     8281 atttagtcca tatagtgtcg aaaaagaata tggtgtccca ttctcttatg taaatattac
     8341 agctgagtat gatcatctag ttgcaaatcc aaacattcgc aaacaaaaaa tcaaagcgcg
     8401 tgatcttgaa aatgaaatct caaaattaca acaagaatct ggctatcctt acgtaatcaa
     8461 tattgataca gcgaaccgcg agaatccaat cgatggcaag atcatcatga gtaatttatg
     8521 ttcagagatt ttacaagttc aaacgccttc tgtgattaac ggcaaacagg aatatgaagt
     8581 attaggaaca gatatctcat gtaacttagg ctcgacaaat atcgtgaatt tgatgcaaag
     8641 ccctaacttt ggtcaatctg tgcgtacgat gactcgtgca ttgacattta ttacagatgc
     8701 atctgatatc gatgtcgtac caccgatcca aaacggaaat aagctgaatc atacgattgg
     8761 attaggtgcc atgggcttac acacttatct tgcaaaagaa caaatggaat atggctcaga
     8821 ggattcatta gactttacaa atatttactt catgctgttg aattattgga cacttgttga
     8881 aagcaacaat atcgctcgtg aaagaaaaca agttttccat aattttgaaa aatcagctta
     8941 tgctgacggc acttatttcg aaaaatacgt aactggagaa ttccagccaa aatcagaaaa
     9001 agtcaaagca ttgtttgaag gcatttttat cccaactgct gaagattgga atgctttgaa
     9061 acaagcagta atgaaagatg gactgtatca tcaaaatcgc ttggcagtag caccaaatgg
     9121 ctcaatttca tatatcaacg atacgagtgc aagtattcat cctatcactc gcatgatcga
     9181 agaacgccaa gagaagaaaa tcggtaaaat ttattaccca gcgccttatc tatccaatga
     9241 gacgattccg tattacactt ctgcttatga tatggacatg cgtaaagtaa tcgatgttta
     9301 tgcaacggca caacaacacg tcgatcaagg catgagtttg actttattca tgcgttctga
     9361 aatcccagaa ggcatttatg aatggaagac aacaacgaag caaacgacac gtgatttgaa
     9421 tattttaaga cattacgctt tccacaaagg aatcaaatcc atttattacg tacgtacctt
     9481 tactgatgat gcagaagaaa tcggaagcaa tcaatgtgaa agctgcgtga tctaatatca
     9541 tagatagatt cgcttattca tattttaatt aaaggagcat tctcatgtca gaaacatatt
     9601 atgaagcaat aaactggaat gaaattgaag atatcattga taaatccact tgggagaagc
     9661 tgacagagca attttggcta gatacacgta tcccactttc aaatgaccta gatgattggc
     9721 gtacattgtc tcaattggaa aaagatacag taggacatgt atttggcggt ttgacactgc
     9781 tagatactgt ccaatctgaa agtggcatgg atcagttgcg taaagatgtc cgcactcccc
     9841 atgaagaagc cgttttgaac aatattcaat tcatggaatc cgttcatgcc aaaagttatt
     9901 cttctatttt tagtacactg aatacgaaaa aagaaatcga agaaattttt gactggacaa
     9961 atacgaatcc ttatctgcaa aaaaaagctg agcgtatcaa tgaaatctac aaacacggta
    10021 caccgctaga aaagaaaatt gccagcgtct ttttagaaac attcttgttc tattcaggtt
    10081 tttatactcc tctttattat ttaggaaaca acaaattggc taacgtagca gagatcatca
    10141 aactaatcat ccgagatgaa tctgtacatg gaacctacat cggttacaaa ttccaattag
    10201 gattcaacga gttacctgaa gcagaacaag aaacgttaaa agactggatg tacaatcttc
    10261 tttatgagct ttacgaaaac gaagaacgtt atacagaaga attatacgat ccaatcggct
    10321 ggacagaaga agtcaagaca ttcctacgct ataatgccaa caaagcattg atgaacctag
    10381 ggatggatcc actctttcca gatactgcca atgatgtcaa cccaatcgta atgaacggga
    10441 tttcaacagg cacaagcaac catgacttct tctcacaagt cggaaacggc tatttgttag
    10501 gtcatgtaga agcgatgaaa gacgacgatt atttgattgg attggaataa ggatttattt
    10561 taattcaaaa atttttcgaa aaataaaaag agtttagcgt acagatattt taaatgtaca
    10621 ctgaactctt ttttatgctt attcacttgt ttcttccagc acttttggac tgtctaactt
    10681 ttccgaaaca tgttccattc ttctcaataa atagattttc cggatattta atattatcgt
    10741 cttaatcact gcatagacag gtatgcaaat cagcatgcct aatattcctg cgacacttcc
    10801 tgctccaatc aataccaaga tgatcgtcaa aggatggata ttcattgatt tcccaaataa
    10861 caacggcttg atcaaatttc catctaattg ttggatcact aaaatcgaca acgccatata
    10921 caaagcttga aatggagaga taaacaaacc gacaatgatt gctggtaccg ctccaataaa
    10981 agggccgaca tacgggataa tattcgtgat tccgcaaatg accgcaagca acaagctata
    11041 aggctgatcg aatattctca ttgccaaaaa gctcattaca ccaataatca aagcatctaa
    11101 gatcgtactg ctgatatagg cagataatgt ttcgtttaat tcttttaccg tttggcgtaa
    11161 ctctttacga atcgctttcg gaaagaagtg agacaacgca tcaataaatt tgtgcccatc
    11221 cttgaacata aatagcaaaa taaatggaac ggtaaataac aaaataaaaa attgcatcat
    11281 aaatgaaatg atttttgaga cacttgcaga taaactgact aatatgatat tgaacaaatt
    11341 actaagagat aaatttgctg atgctaattg ttcttccaaa ttgaaatttt taaactcttc
    11401 ccgctgcgcc aattcattta accatttgct tgtttcatct gcatacattg gcaagctctg
    11461 ggtcaattga attgtctgtt ccattaactg aggcactaca ttcatgacag ttaaaacaac
    11521 taaaatgatt acgaccaaaa acgtcaataa aaaaccgaac actctcggga tccgatgctt
    11581 ttcgagaaaa acaacgattg gatcgaacat ataatagata aaaccagcaa caagcaatgg
    11641 taaaaaaatt gccgaaagca ttttcaatat tggactaaaa atattcggca tttgtaaaat
    11701 aaagtacacg ccgatgatcg tgagtactag ctccactgtc cagaaaaata attttgattg
    11761 tttaaaacgt gattgcatac atctcctcca aggtcctttg atacttctat tctatcattt
    11821 aacaaagagt tacgcgctga gattttcatt tgcttaaaaa aaacaaaaaa aataaacata
    11881 ttttaatgag tagacgagta cttaaacaaa aacacggttt ggagtagtga caaaaattat
    11941 actgcagtcc ctttttccaa ataaacggtg ttcaaaagct tgcatcttcg taataagccc
    12001 agccaaacga aaaatataag gaactatttt tatttggctg ggcttattac ttgatgtagg
    12061 acatctattt tacaacctca gatcatttat tgcgcaatac tgactaaatg caactttgtg
    12121 atatttttgt gtacggacaa ctcctcgatc acatcggcat aagtgagccc ttttggaaga
    12181 tcaatcgtat agatattttt gtacactcgg tagtcatcaa cgaattttac gtcaaaattc
    12241 acatcttcga ttacgatatt tttctcttca aaataatcgg taagaaaatc tttggtttct
    12301 tttctatgga taaattggat ttctaacttt ttagtcgtag gtacatggat cacgcgtttc
    12361 actactgtga gtgcaatagc aatcccgata aaactgatga tcgcaatgga atagtaaccc
    12421 atgccgatgg cgatccctaa tccagcaatt gcccataaag aagcggcagt cgtaagtccc
    12481 gtcaccgatt gtttcgtgac gatgatcgtt cccgcgccta agaaaccgac accactcacc
    12541 acttgagcaa tcagccttgc ttcatctgaa cgaatcgttc cagcaagctg tggatactct
    12601 aaagcatctc ttaatgcacc actagcgatc tcaacttgga tcaatgcaat gatacatgct
    12661 cccatacaaa ctaaaatatg cgtacgcata cctgctggac gatttttata ttgtcgttca
    12721 aaaccgattg ctccgccaaa tagcatagcg atccctaaac gtaaaataat ctctggaatt
    12781 gtcaatacca tatcattcat ctaaacaccc tccagtccaa tatatcatgt ctgaaaggag
    12841 cagacaaaaa aagtgcaatt tttacctctt acgaaaaaag gactgtgaca aaatttttgt
    12901 catagtcctt tttatgaata aacggtgttc aatcagctga ccctcggtat tactcaacaa
    12961 acggcaaaat atggggagct atttttcctt tgtcttctct aatactcagg tcaaaacgct
    13021 gatttcacaa cctctttagc ctactattga aaattacaac ttacttgctg cttcttcatc
    13081 gatgatcaca acaacattgt cgtgattttg caatacactt gctggcaaat gattcgttac
    13141 aggtccttca atcatccctt tgattgcatc agctttgctt tcgccaaatg ccatcaagac
    13201 gatttcttta cttttcataa ttgaaccgat tcccattgaa acagcttttg ttggtacatc
    13261 ttctgcttta tcgaagaatc gtttgtttgc attgattgtt gattctgtta agtcaactac
    13321 tgaagtcgtt ccgtcaaatg aagcaccagg ttcattgaaa ccgatatgtc cattttgccc
    13381 gattcctaag atttggatat cgattggatg tgcatcgatg atttgatcat actttttgca
    13441 tgctgcttcc agatcttctg cttttccatc tggtacaaac gtttctttga atggtttttt
    13501 atcaaataat tggacatcca tgaaatgacg gtagctttgt tcatctgaac cgcctagtcc
    13561 aacgtactca tctaagttta cagaagtcat ttcactgaaa tccaaatcgc ttgaagtcat
    13621 ttctttatat aaagtaactg gtgtgcttcc tgttgccaat cctaatactt ttgcaccttt
    13681 gttcatacct tctttgatga tttcaaatgc tttttttccg ccttcttcag cgttttttac
    13741 acgaatgatt tccatagtga ttgctccttt atattttggt atagcccaat tatagcattc
    13801 tttttttctt tttacaataa ttagtataga ccaaattgca tttttttcat tttcttgaca
    13861 ataaatagga atccgtgtta ggtttcataa gatggttcaa tcaaacaatc catgttgcat
    13921 gaaaaccaag caagtgattc actgtggacg ctttatgtct gcagtttttt tatgaaaaag
    13981 gagttattat gagtatactg acattaaaca acattaccca gcaatttggc gacaaaatcc
    14041 tctatgaagg tgtcgatctg caattaaatg ctggcgaaca tcttgggctg attggacaaa
    14101 acggagccgg taaaagtaca ttgattaaaa tcatcactgg tgagatctta ccagatgacg
    14161 gacagatcca ttggcaaaaa aatattcaca aaggttacct cgatcaatat gtggaagtcg
    14221 atgaaacatt aacaatcgaa gaatttctaa aaactgcgta cgcaaaagaa tacgaaaagg
    14281 aagaaaaaat tgcttttctt taccaaacat atagcgagac cttcgatgat tccttgctgg
    14341 aaaaagctgg gaaactccaa acggaattag accaaggagt gttttatcaa atcgacacct
    14401 tagttgctga gatgagcagc gggttgggga tcgatgtctt aggtttagat tcactgttga
    14461 aaaatctaag tggtggacaa cgttcaaagg tgattttagc aaaactactt ttagaaaacc
    14521 cagatgttct gatcttagat gaaccaacca atcacttaga tgatcagcat atccaatggc
    14581 tgacgcagtt tcttcaggat tttgacggtg cgtatatcgt gatttcccat gaccaagaat
    14641 ttcttgatca gatcaccacc catatcgcag acatcgagtt tggaaagatt actaagtata
    14701 ctggccatct aaaacaagca ttgaaacaaa aggaacaaaa tcgcgaaagc tatttgcgcc
    14761 aatatcatgc tcaacaaaaa caaatcgaaa agaccgaagc ttatattcga aaatacaaag
    14821 ccggttcccg atcaacgatg gcaaaaagcc gacaaaaaca attagataaa atcgaacggc
    14881 tgacgcctcc agcttcagca agtaaagctg tctttgattt tccattctcg cctattgtca
    14941 cgacattagc agtggaaaca actgatttag tcatcggata tgatcgaccg ctacttgcac
    15001 ccatcaattt gaccatccgc tttggtgaaa atattgccat tcgaggcttc aacgggattg
    15061 gaaaatctac tttattaaaa acactgatcg gtgagattga aaaaataagt ggcgatttcc
    15121 acttcccaga taacacaaaa atcaattact tttcacaaga tctgtactgg gaaaatccat
    15181 tagaaacacc actacaatat ttaagcagtc aatttccaaa agcgactatc aaagaattac
    15241 gtcgccaact agctaaagca gggttagtta atcaattagc aagtgaacct ctcactacat
    15301 taagtggtgg ggaacaaact aaggtgaaac ttgcacagtt gacaatgaac caaggtaatt
    15361 tactgattct cgatgaaccg acgaaccata tcgatcaaga aacgaaagaa agcttacaag
    15421 acgggataca gaaatttcca ggaacagtaa tcatcgtctc tcatgaacaa gaattttatc
    15481 aagatcttgt gcaacgagtc attgagattg aaccaaaata aacgtttgat tgaatagttg
    15541 aaaaaaagga gctgtgacaa aagttttgtc acaactcctt cttctgaata aatggtggcc
    15601 aattagctga ccttcggtat tagctcaaaa aacggcaaaa tatgaggagc tatttttatt
    15661 tggctattct tattacttga tgcagaacag cttttcacaa cctcttttcg tctatttttt
    15721 attttcgaga tttctctttc ctttaaaaac aactgtttga aagaagaacg cacatccttg
    15781 aaaaacaagc ccgctaaccg ttacacctaa ccagccatac tgctgccaca ttgtcgtccc
    15841 tagcaatgaa ccagccgcac ctccaataaa gtacataaac ataaataca
//
LOCUS       DACCXO010000059        15367 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.060, whole genome shotgun sequence.
ACCESSION   DACCXO010000059 DACCXO010000000
VERSION     DACCXO010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.060"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            288..695
                     /locus_tag="IVY70_001717"
     CDS             288..695
                     /locus_tag="IVY70_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958847.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="IVY70_001718"
     CDS             709..1110
                     /locus_tag="IVY70_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958848.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..1483
                     /locus_tag="IVY70_001719"
     CDS             1112..1483
                     /locus_tag="IVY70_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958849.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1519..1824)
                     /locus_tag="IVY70_001720"
     CDS             complement(1519..1824)
                     /locus_tag="IVY70_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958850.1"
                     /translation="MTIELEEHLLWFKYDPSEKFVKDLYKVWDTEVVFLAIETSLLVN
                     LHYSNKNYFKIPAVKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(2351..2423)
                     /locus_tag="IVY70_001721"
     tRNA            complement(2351..2423)
                     /locus_tag="IVY70_001721"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2388..2390),aa:Lys,seq:ctt)
     gene            2804..3373
                     /locus_tag="IVY70_001722"
     CDS             2804..3373
                     /locus_tag="IVY70_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP7958851.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            3360..4220
                     /locus_tag="IVY70_001723"
     CDS             3360..4220
                     /locus_tag="IVY70_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7958852.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            4410..5114
                     /locus_tag="IVY70_001724"
     CDS             4410..5114
                     /locus_tag="IVY70_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7958853.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            5119..6954
                     /gene="walK"
                     /locus_tag="IVY70_001725"
     CDS             5119..6954
                     /gene="walK"
                     /locus_tag="IVY70_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP7958854.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            6951..8267
                     /locus_tag="IVY70_001726"
     CDS             6951..8267
                     /locus_tag="IVY70_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958855.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            8268..9137
                     /locus_tag="IVY70_001727"
     CDS             8268..9137
                     /locus_tag="IVY70_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958856.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            9192..10001
                     /locus_tag="IVY70_001728"
     CDS             9192..10001
                     /locus_tag="IVY70_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7958857.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(10104..10733)
                     /locus_tag="IVY70_001729"
     CDS             complement(10104..10733)
                     /locus_tag="IVY70_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAP7958858.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            10903..12195
                     /locus_tag="IVY70_001730"
     CDS             10903..12195
                     /locus_tag="IVY70_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7958859.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(12271..12933)
                     /locus_tag="IVY70_001731"
     CDS             complement(12271..12933)
                     /locus_tag="IVY70_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP7958860.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            13158..13442
                     /locus_tag="IVY70_001732"
     CDS             13158..13442
                     /locus_tag="IVY70_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP7958861.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            13493..15118
                     /gene="groL"
                     /locus_tag="IVY70_001733"
     CDS             13493..15118
                     /gene="groL"
                     /locus_tag="IVY70_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP7958862.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         5204 a         2568 c         3215 g         4380 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataaa caaactcacc taatgaattt caagcgagtt
     1501 tgtttgttat ttttttattt aatctttgta tctttctgga tcaacgaaag tatactttat
     1561 atagtcataa cgccgatgat cgctacgtgc gtccggcacg tcagtcacga tatcaaacaa
     1621 aaagtataca cccttcttca ttctcgtttt cacagcagga attttaaagt agttcttatt
     1681 agaataatgg agattgacta gcaggctagt ttcaattgct aaaaagacta cttcagtatc
     1741 ccagacttta tataaatctt tgacaaattt ttctgatgga tcgtatttaa accaaagcag
     1801 atgttcctct agctcgattg tcatgttttt cacctcaacg aaagtatacg aactaatgtt
     1861 tgttttgtaa aggataaaaa tagggtagca accggtaccc aaactagtac ccatttatca
     1921 aaaacgacga aatttatgta aatagaaaaa taagaataaa cattgatgta tcaacattta
     1981 tatatcttta gaacaaatga aaagggtggt tctcataaga aaaacatgtt cgcacttcta
     2041 ggcaaaccag gatttgaaga ccttgctaaa gaattgaacg aacgcctgta aaataaggat
     2101 ttgtcgctag atttaaaaaa tcagtgatag aaacatgttt gctttaaaat gaaactagtg
     2161 atagttttag tgatagtgaa cttaaaaaaa gcactcaatt tgagtgcttt tttattgtta
     2221 ttaaaaatta aaaaactaaa atacataccc gaatttcata cccgaaatat aaaaaatgtc
     2281 atcacgtaca tctttttcga aactaccgaa gtagccaaag atgtactgat gacgtgggtt
     2341 tggcacccaa tgggccgtga ggggctcgaa cccgcgaccc gctgattaag agtcagctgc
     2401 tctaccaact gagctaacgg cccgtacaaa aagtacttaa taatagtagc actaagaatt
     2461 ttaaaatgca agactacatt taaagaaaat cagaaaaatc tttacttcct tataggaaat
     2521 cattcataga ttattcacat ttgctcgcta ttatataagc ataccaacaa acaaagttct
     2581 tgcaagagaa caaaccctaa cacttttttc attttttact cctttcccgc cgtctccccg
     2641 gcgggtctcc tgttcttaac gataaaagac aagaaggctt agccttcttg ttttttgttt
     2701 tcatggaatc ccgctaatca aatcacctag ttaaagcaac tagattgaat gtttatcata
     2761 agtctgatat catgtttatg aaatgaaaaa aggagcggtt atgatggaaa aaatgcgtca
     2821 gcaattaatg gagtggggga aggaactaga aacattccgt ctgcctcact gggaagaact
     2881 ccctgactta gaactttata tggaccaagt cagaacgtta gttgatcgtt atctttcacc
     2941 tgtgatacaa ggagaaaagc atccattgct tacctcttct atggtaaaca attacgttaa
     3001 actaggtttg ataccagcac cagttaaaaa aagatataac aaagaacacg tagcattttt
     3061 acttgcaatc actacgttga aacaagtatt gactattcct gaaatcaaag aagggatatt
     3121 gttccaagga aaaacagtag gaattcgtga agcctataat ctattttgcg atgaacaaga
     3181 agcagctgtg tggatggtca gccaattagc acaaggtaaa tctcatccgc aaaaattcga
     3241 ccaggcaacc cctgtagaat atattgctgt aagagcggca actttatcgt ttgcaatgaa
     3301 acttcttgcg gaaaaaacga ttgttctaga aaccgaatat ttaaaagagg agaaaacaaa
     3361 tgaaaaacaa taaaatcgca attttagtag attcaggtac ggatgtgcca gaagaattga
     3421 taaaaaaata cgagatgtat atgatcccat tgaaaattat ttataaagat caggtatata
     3481 cagataaaat tgacattacc tctgaagaga tctatcaacg tttgccagag gaaatccctg
     3541 gtacatcttt gccggatggt gagtcgatca cagaaatctt tgagcaaatc aaaaaagacg
     3601 gttatgaaaa ggtacttgcg gtaacaattt ccagtggatt gagcgggaca tataatgtgg
     3661 ttcgtttaat tgcacaacag caagaagaat tggaagcata tgtactagat acgaaaaata
     3721 ttggtattgg tgcaggtttt tctgcgatcc aagcagcaaa atggctggaa gaaggagtag
     3781 aatgggatca actgatcagt aatctgaatg agttagtcga aagaacaaaa gttttcttta
     3841 atgttgcgac attggaatat cttcaaaaag gtggacggat cggtttagtt gcttctattg
     3901 taggaaccgc tttaaagctt aacccaatca tctcatgtaa tgaagaaggg atttattaca
     3961 cagtaggaaa agctagggga cgtaaaaaaa gtttggataa aacggttgct tacgtcaaag
     4021 aaaaaatcgg taatgcaaag gtcttcaact tagctgttgc ccatggggat gcgaaagaag
     4081 aagcaattga aatggaagcg agacttaaaa gagagttccc acaagcaaac caaatctact
     4141 ttggccagat ttctccggca ttagtagtcc acactggtcc gggattattg ggtgtcggtg
     4201 tacaagtttt agaaggataa atcaaaaaaa ggaaacactg aggtgaaaat cagtgttttc
     4261 cttttttatt gaagttagaa caaaaaataa taaagtattt tgcactttca ttttaatttt
     4321 ctcttttgtt ttataggcaa agatgaaaca aaagcagaaa tacggtataa tgagaaggta
     4381 tgacaataat atataagagg tggctcttta tgaaaaaagt tttagttgtc gatgatgaga
     4441 agccgatttc ggatatcgtc aaattcaatt tagccaaaga aggctatgat gtttatactg
     4501 catatgatgg ggaagaagcc ttggaaaaag ttgctgaggt agaacctgat ttgattttac
     4561 ttgatttgat gctacctaaa atggacggct tagaagttgc tcgtgaggta cgcaaaactt
     4621 atgatatgcc gatcattatg gtaacagcca aagattctga aattgataaa gtcctaggct
     4681 tagagttagg cgccgatgat tacgtgacca agccattttc caaccgagaa ttagttgcac
     4741 gtgtcaaagc aaatcttcgt cgcggtgcca cggctgcgaa ggaaccagaa gaggcagcac
     4801 ctgcggaatt gacgattggt gatttgacga tccatccaga agcttacatg gtgacgaaac
     4861 gaggagaaac aatcgagtta acacatcgtg aattcgaact actcttttac ttggcgaagc
     4921 accttggaca agtgatgaca agagaacatt tattacaaac ggtttggggt tatgattatt
     4981 ttggagatgt acgaacagtg gatgtaactg tacgtcgttt gcgtgaaaag atcgaagata
     5041 atccaagcca tccaaattat ttagtgacac gtcgtggtgt cggttactat ttgcgtaacc
     5101 cagaacagga gtaattggat gaaaagaaaa gtccgctttt ttcgatcagt caattttaag
     5161 atcgctatta cctttattct gattttattg atctcgatcg aaattattgg tgcctacttt
     5221 atacgtggtt tagaacgttc cacgatcaat acatttatta aagatatgaa tcaaacggtg
     5281 gaatcattag caacaacaat cagcccggaa ttgaatcgaa aagataatgc agatgatgaa
     5341 gaggtaaatg cgaatatcaa aagatttatt gaaaacagtg cgactagcga tatcatcgaa
     5401 atacgggtcg ttgacgaaaa agggatcatc cgaggaacaa ccgatgtcaa tgaacaaagc
     5461 gctgtgggaa agaaaaatga ttatgttgat atcaatgatt tcacgactaa acgttatgtt
     5521 gctttagata acgacgataa acgggtgaat atcaatgttc aaccgatcct gtccccgacc
     5581 ggcgatgccg ttatcggggc tctttacgtc aaaagtaata tcgaacaaaa atattcggaa
     5641 atcaataaca cagcagtcat tttctttact gcctcactaa ttgcggcatt tatttcaatg
     5701 atcgtatctg ttttagttgc tcgttcgatt acacagccga tcggtgaaat gagagaacaa
     5761 gccattcgaa tcgctagagg agactacagt cggaaagtca aagtgcatgg acaagatgaa
     5821 cttggacagc tggctgatac cttcaatcag ttagctgaac gtattgaaga agctcaagat
     5881 acaatggaag ctgaacgcaa tcgtttggat agcgtgctat cccatatgac agacggcgtc
     5941 attgcgacag atcgcagggg taaagtcatt acgatcaatg atatggctgt ttccttgcta
     6001 gatatcaaaa acgaggaagc aatcggacaa tcgattttga cacttttaga tatcgaagaa
     6061 gaatatacgt tgcggaaatt attagaagat ccaaacgaaa tggtacttga ccgttcaacc
     6121 agtacattgg aaagtgatca gatgatcttg cgagtagact tttctatgat ccgtcgcgaa
     6181 tcaggtttta tcagcggatt agtagctgtc ttgcatgatg tgacagaaca agaaaaaaca
     6241 gaacgggaac gtcgtgaatt cgtctctaat gtttcccatg aacttcgtac accgttgaca
     6301 agtatgcgta gttatatcga agcattgagc gaaggtgcgt ggaaagacga ggaagtagca
     6361 ccaaattttt tgaaggtaac gctagatgaa acagaccgaa tgattcgaat gatcaatgac
     6421 ctcttaaatc tatctcgcat ggatacaggt aatacccagc ttcaattaga atatgtcaat
     6481 ttcaacgagt tagtgaattt tgttcttgac cggtttgata tgatggtggg aaatcaggaa
     6541 aaaaattata agatccgtcg tgaatttacg caaagagatc tttgggtcga agtagataca
     6601 gataaaatca tccaagtagt cgataatatc atgaataacg cgatcaagta ttcaccagat
     6661 gggggaacga ttacttgtcg cttattagaa acgcataata acgtggtttt aagtatcacc
     6721 gatcaaggat tggggattcc taaaaaagat ctgaaccgag tgttcgagcg gttctatcga
     6781 gtagataaag cacgtgcaag agcacaaggt gggacaggac ttggattagc tatttctaga
     6841 gaagtgatta aggcgcatcg aggcgctatt tgggcagaga gcaaggaagg aaaagggtcg
     6901 actttttata tctcgctgcc atatgaacct tatgaggagg aatggtggga atgagaatct
     6961 ctgaaaaagt cgttcgtata ggactcattt tgatggttgc gttaagcata tacttctctt
     7021 atgcaatctg gctgagccca gcaggaaaaa gttcaatcaa cgtagatgag acaaacactc
     7081 aaatgatcga gtctcaaagc aatcgaaaaa cttccgaagt ctttcttccg cttcatgtta
     7141 catggcacca ttcgggaatg atccaagaaa ccaacagtga aaatctcgtg taccgattgc
     7201 aattgatcat cgataaagca agatttggaa agctttctga agtagtttca ggagacaaat
     7261 cacagtttga acaggtgaaa aatctgaact ctggttttga gttggcctat aatgcgcctt
     7321 ttttgctaag agaatacaag gaagcatttg atttagattt agatgtgtcg aacttggcaa
     7381 aagaaagcga aaatatgtat tttactagaa tacaattcga tcaaggaaat aaaaaaatcc
     7441 gatttttgaa ttattcgaac catctcgtat atgaagcaaa tatttcgttg aattggacag
     7501 atatcaataa agaattaaca gcaaaagaca caaaatggac agaaatgaca acagaaccat
     7561 cgacgatcga cacgcaatat gctacgaaac attcgatcaa actgaaaaag tatagttata
     7621 tcctgtcaca gcaaccctat acacttttta gaaatgcttt tttccagaaa ccagatagcg
     7681 tgaaaaataa tgatgaaagc aatgaattga ttttttacgg tgggaatgaa acaatgtcta
     7741 tccattcgga aacgcaagta gttgattttc gtggagagct gccagagaaa aaagaaaatg
     7801 ctagtgtatt ttctgaaagt ttcccctata ttagcaaatt aggcagttct ttagggaacc
     7861 tcagatattt tgaccgcagt gacaatacga tcgattatcg gatattcgtg gaaggcttcc
     7921 cggtttttgg gacagacagc agaggacaga ttggtgtgga agttggcggt gaaacgaatg
     7981 gttctgtgca agtgaatatc caaaccagtt tgaatacgat tcaagtgccg ataccttcag
     8041 acgaagaagt agaacttgca agtacgaatg aaatcatcca gcagttaaaa gctaagggag
     8101 cagatagcaa caagattggt tcagtgatcg ttggctatac atggcaaaat gtcaaagaaa
     8161 cgaatcgtgt cgttgatctc ttacctgaat ggtatgtgaa atataaagat aactggtatt
     8221 cagctaacga ccttctggaa aaattacctg gattggaggc gaattaattg gatttcaaaa
     8281 aaatcgaatg gatatttttc gtcgcttttt tagggttgaa tgtgttttta ttcagcagtt
     8341 atcatgacgc ggtataccaa gaaaacaatg tcattcgctc taatcagact gaatcaatcc
     8401 aacaacgtct ggctagcgac gatatcactt acacggggaa gatttcgtct gagaacaagc
     8461 aaggctatta tcttagcgcg gagcagacca atcttagcac ttcattagcg aattatcgca
     8521 aggctggtaa aagcggcttg ctggcaagtg gtgtgttttt agacggtgat gttttgacga
     8581 aatctttatc cagttcagat gctgaaaaga acgtgatcga tccagataaa attagtagtt
     8641 ccttggaaga ttttttatct aacaaagaca atattttatt cggtaatgat tatgtgtaca
     8701 cgaaaaactt ttctcattta gaagaagaac cactagaaat ccaagcaagc caagagtatg
     8761 aaggcatccc attcaatgat gatacagcaa aaatcgttct ttctttagaa aaaaacgata
     8821 atgattatag catcacgaaa tacatgcaga cgcatttatc tgatatcgag cagctgcgcg
     8881 aaaagacgga actgtatacg gaagaggaag caatcaatac attgtacatc aataacaaaa
     8941 taccaagagg atcaaaaatt ttatggcgtc agctagctta ttcctgtatt ctgaaagtcc
     9001 gggaaaaaaa tgtgtatgtt cctgtttggc atgtagcgat cgaaacaagc gataaagtgg
     9061 tccaaattga gagtgtcaac gctttcagca atacgatcgt gacaaacaat actgtaccaa
     9121 aagtggaaga tcaataaaaa agcgagtata atgaaaaaag tttacaaagt aagaagggaa
     9181 gtaggagtca aatggctgaa gaaaaaccag cttttaaaat cagtatccta gcaagtggaa
     9241 gttcgggtaa ttcactttat atcgaaagtg aaaaaaaacg gcttttggtc gatgcaggac
     9301 ttagcggtaa aaaaatcact tccttgatgg ctcaaattga tcggacaccg gaccaattag
     9361 atggaatact tgtgacgcat gaacatcgag atcatataca tggcgtgggt gtcttggctc
     9421 gtaagtatca tttggatgtt tttgccaatc agcagacttg ggaagcgatg gaaccactga
     9481 ttggaaatgt accagtggaa caaaaacatc tatttgagat ggggaaagtc agaacattcg
     9541 gcgatatcga tattgagagt ttcggcgtct cccatgacgc agcggctccg caattttatc
     9601 gtttccataa agaaaatcgg tcattcgtca tgttgacgga caccggatat tgcagtgatt
     9661 atatgcgagg tgtcattgaa aatgccgatg gttatttgat ggaaagtaat catgatcttg
     9721 agatgttacg catgggacct tatccgtgga atttaaaaca gcgtatttta ggagatagag
     9781 gacatctttc caacgaagac ggtgcacttg tcatgactga tgtgatcggt aatcggacca
     9841 aacgaatcta tcttggccat cttagcaagg aaaacaatat gaaagaactt gctcatttaa
     9901 caatggaaaa tgttttgaaa gaaaaagatt ttggcgttgg acatgatttt gaaatctatg
     9961 atacagatcc agatgaagca actgagctat tttcgattta aacagttcaa tcaataaaaa
    10021 tataaaaagc agaagataaa atcaatcagg gttggtttta tcttctattt ttattttttt
    10081 agcatattaa aaaaataacc gtattatcga ctgactacac tgatgtgctg cttttcatgt
    10141 ttctcatcca gtatctcatc tactaatgca atcgcgtaat ctgccatgct gatcgtactg
    10201 tcgccagcag cgtttctttg taaataatca tcacttaaca aataagtacc tgtacgtttg
    10261 ccatgtggat caaataaaga agctggactg atataagtcc aatgtagatt tttagattgt
    10321 ttcaatttta acaatccttc gtacatattg aaagctgttg gatagaaggg tgcattttta
    10381 ggtacttggt caatcatacg agtcgtcttt tctgtatcta agaataacga agaggcaccc
    10441 ccaacgacaa ttaagcgtgt atcggttcct tcaagcatct caatcaaatg ggacaaagaa
    10501 gtttgatgca tttcttcttt tcctttggga gcgttgaaag catcaacgac aacatcaaaa
    10561 gatgcaagat cagtagttgt caactcaaat aaatcctttt gtataaacgg aacatctgat
    10621 ggcaattttt ctggatgacg cacgattgcc gtcacgtcta aatctctgga caatgcttcg
    10681 tctaaaataa atgaacctgc atggccagta gctccaatga gtgcaatttt cataaaaaac
    10741 ctcctttttt attcctgtcc attgtaagca gttgctgaat attcgtcaaa gaatccgatt
    10801 tttgaaaagc tcagtgcgca ttcctttcat ggttcgtgat cattgggtat agttaaatta
    10861 agaaaactga ttgataaaaa tagaagttga aggagataag aaatgaaaga gtggttagat
    10921 cgtgcctttt taccaatgat aacatctgtt gtgaatctta gacccttaac tgctttgcgt
    10981 aatggaatga cttacacgat cccaataatt attgttggct cgctgttttt cttatttgct
    11041 cattttcctg tggattacat cgctcagtgg atagacaaaa caggattttc tatgtatttt
    11101 acacaagtat accaggctac atttgatgtg atagcctttt ggatcgtttt tgccattgca
    11161 tacgcttatg caaaagaaga agacatcgat cgcttaccag tcggcataac agccttgtct
    11221 gcttatctac tgcttattca gccactaaat actcagatag atactgtctg gacaggtgct
    11281 gaagggatac taggagcagt tacaactggg ctcatcatcg gatggggcta tgcacgttta
    11341 gtaagacttg cgaaagggtg gaagcaaaga gagaagatac ctgaaaatgt ttggcagtct
    11401 tttacttcat tgattccaat cattgttata gtcactttta cgcttatcgg ttcagcggtt
    11461 tttcaacagt tgttcagaat gactgttgtt caaggagtat ggacatatgt ccagcttccc
    11521 attcaatcgt tcatagacac gttgtttggt gtgcttttat taggtttttt tgttccgttt
    11581 ttatggttat ttggtcttca tggatctgcg atggtcaatg ggttggttag tcccgtcttg
    11641 caagcgaatt cattagcgaa tgcagaaatc ttagcctcag gacaagagtt gacggtggca
    11701 aatggcggac atatcgttac tcagcaattt ctcgatcagt tcatgacggt tacgggtgct
    11761 ggattgacgt taggggctgt attttttatg acggtttttg cgaaatcgaa gaaatacaga
    11821 gaattaggaa atcttgctct tttaccagcc tttttcaaca tcaatgaatc aattattttt
    11881 tccacaccga tcgtgatgaa cccgatgatg gctgttcctt ttattttcgc accggtcgtt
    11941 tcaggattga ttacctattc cgctttatat tttggattcg ttcctttatt tacagcagtc
    12001 caagtccctt ggacaacacc gccgatcctc tcgggtttct tagtaggcgg agtgcctgca
    12061 gcgattttac aaggtatcgt gttgtcaatt agttttttca tttatttccc atttttgaaa
    12121 aaaatagatt ctgcaaactg caaaaaggaa agacagacaa aaaatgatgg agcagcagaa
    12181 aagatgaacg actaaaaaac aaaaatactc ctgtcgcttt agattgggaa aatcaccaat
    12241 ctaggatgac aggagtattt atagtcatgt ttatactagc cctttttgga cagctaattg
    12301 aacaagaaca actaatgtgt tcattcctac gtgtgtgatc atcgatgtcc agatccgacc
    12361 agttgctttg tactctaggc tgaagaagaa acctaggaaa aagtagatta gcagatgacc
    12421 atcattatga gcaaacgcaa acagcaaaga gctgatcact gctccgaccc aaaaatttga
    12481 atatcgactg atgatcccca gtatactgcg gcggaatacg aactcttcca tgatgggtcc
    12541 agcaacagtt gtcgccaaaa taaaagcggg tgcttccatg atcatctgga tgatattttg
    12601 tgtattctcg gaaggggttg ctttgccaaa caatacagat tctaacatga cagcgatgct
    12661 ttgcagaatc aatgcgacaa agattccaac caatccataa ataacaatct ttggagcaga
    12721 agcttttttt accgctgatt cgatcgctaa gggttcacgt tgcttaaaat aaaaaatagc
    12781 taggacaacg gcgcctagta tatacatgac tgcagttgct gtgatcaatt cagacgtaga
    12841 ttttacaagt cctatcgaag ctaggatcaa gggtgagata aaaattagac catagcaaaa
    12901 aatgctgagg aagctatatt tttttaaaga catgtaattc ctccttttaa gggataacaa
    12961 gcatgcaagg caagcttata tcgattgatt tcaattatat cataatttca ataagcagct
    13021 gtcagaataa aatttctttt ctttgattta aaagcttgca aaaaagaggg agaatgagta
    13081 ttattaaaaa tgtaggttag cactcgaagg ttaagagtgc taacagatta aaaaaagagt
    13141 tatcggaggg atttatcgtg ttaaaaccat taggtgatcg cgtcattatc gaagtcgcaa
    13201 aagaagaaga aaaaactgtc ggaggaatcg ttttagcttc tgctgctaaa gagaaacccc
    13261 aaactgggac agttgttgca gtcggtgaag gccgtttgtt agaaaacggt gaaaaagttc
    13321 ctgccgctgt aaaagcaggc gaccaagtta tgttcgaaaa atacgccggt acagaagtaa
    13381 aatacgaagg aaaagaatat ctgatcgtcg caggaaaaga tatcatggcg attgttgagt
    13441 aaatttggaa gataaaaaaa taatcaaaaa taatcaatga ggtgaatcaa tcatggcaaa
    13501 agaattgaaa tttgcagaag acgcacgcgc agctatgtta cgcggggtag ataaattagc
    13561 agatacagtg aaagtaacat taggacctaa aggccgaaat gtcgtactag aaaaatctta
    13621 cggctctcca ttgatcacaa atgatggtgt gacgatcgca aaagaaatcg aattagaaga
    13681 tcattttgaa aacatgggtg caaaacttgt atcagaagta gcatcaaaaa caaatgatat
    13741 tgcaggagac ggtacaacga cagcaactgt tctgacacaa gcaatcgttc gcgaaggttt
    13801 aaaaaacgta actgcaggtg ctaatcctct aggcattcgt cgcgggatcg aattggcaac
    13861 aaaagcagca gtcgaagaat tgcataatat ctcaactgtt gttgattcaa aagaagccat
    13921 tgcacaagta gcagctgttt cttcgggttc tgataaagtt ggacatttga ttgctgatgc
    13981 aatggaaaaa gttggaaacg acggtgtcat cacaatcgaa gaatcaaaag ggatcgaaac
    14041 agaactagat gttgtagaag gtatgcagtt tgatcgtggt tacttatcac aatatatggt
    14101 aacagataac gacaaaatgg aagctgttct agaaaaccca tatatcttga tcacagacaa
    14161 aaaaatctct aacatccaag atattctgcc attgttagaa caaatcttgc aacaatcacg
    14221 tccattgttg attattgctg acgatgtaga tggtgaagca ttgccaactc ttgtattgaa
    14281 caaaatccgt ggaacattca acgtagtagc tgtaaaagct cctggtttcg gcgatcgtcg
    14341 taaagcaatg cttgaagata tcgctatttt gacaggtggt acagtaatta ctgacgatct
    14401 aggtttagaa ttgaaagatg taacaatcga aaacttagga aatgcttcta aagttgttgt
    14461 agataaagac aatacaacga tcgtagaagg ttctggagaa aaagaagcaa tcgaagctcg
    14521 tgtgcaattg atcaaaaacc aaattgctga aacaacttct gattttgacc gcgaaaaatt
    14581 acaagaacgc ttagctaaat tagcaggtgg tgtagctgtt gttaaagttg gtgcagctac
    14641 tgaaacagaa ttgaaagaat taaaattacg cattgaagat gcattgaatg ctacacgtgc
    14701 cgcagtagaa gaaggaatgg tctcaggtgg tggtacagct ctagtgaacg ttatcagcaa
    14761 agtttctgct gtagaagcag aaggagacgt ggcaacaggt atcaagatcg ttgttcgtgc
    14821 tttagaagaa ccaattcgtc aaatcgctga aaacgctggt tatgaaggat cagtgattgt
    14881 tgataaattg aagaacgtgg aactaggcac tggtttcaat gctgctactg gagaatgggt
    14941 aaacatggtt gaagcaggta tcgtagaccc aactaaagta acacgttctg ccttgcaaaa
    15001 tgccgcttcc gtttctgcct tgttattaac gacagaagca gtcgttgcag ataaaccaga
    15061 accagcagcg ccagcagctc ctgcaatgga tccatcaatg atgggcggta tgatgtaaga
    15121 aaaagcaact tgcttatcta caactgatca ttagtgaaag acagataaaa agaggctgga
    15181 acagaagcgt ttaactccaa gaaataagaa gaaattcacg gaaattgctt ttcaaatttt
    15241 tgtgaatttc agcttatttc cgaaggagtt gcttctgctt ccgctgttta tacgtgttta
    15301 gagcgtgaga caaaagtgtt ctttactttt gtcccacgct cttttttgtg tgtaattata
    15361 atgaaaa
//
LOCUS       DACCXO010000060        15159 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.061, whole genome shotgun sequence.
ACCESSION   DACCXO010000060 DACCXO010000000
VERSION     DACCXO010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.061"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(159..1469)
                     /locus_tag="IVY70_001734"
     CDS             complement(159..1469)
                     /locus_tag="IVY70_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP7958863.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2899)
                     /locus_tag="IVY70_001735"
     CDS             complement(1481..2899)
                     /locus_tag="IVY70_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7958864.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3139..4707)
                     /locus_tag="IVY70_001736"
     CDS             complement(3139..4707)
                     /locus_tag="IVY70_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP7958865.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4711..5121)
                     /locus_tag="IVY70_001737"
     CDS             complement(4711..5121)
                     /locus_tag="IVY70_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP7958866.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5118..6041)
                     /locus_tag="IVY70_001738"
     CDS             complement(5118..6041)
                     /locus_tag="IVY70_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP7958867.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6038..6916)
                     /locus_tag="IVY70_001739"
     CDS             complement(6038..6916)
                     /locus_tag="IVY70_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP7958868.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRFATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6903..8239)
                     /locus_tag="IVY70_001740"
                     /pseudo
     CDS             complement(6903..8239)
                     /locus_tag="IVY70_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
     gene            complement(8242..9345)
                     /locus_tag="IVY70_001741"
     CDS             complement(8242..9345)
                     /locus_tag="IVY70_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP7958869.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9419..10927)
                     /locus_tag="IVY70_001742"
     CDS             complement(9419..10927)
                     /locus_tag="IVY70_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7958870.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10893..11585)
                     /locus_tag="IVY70_001743"
     CDS             complement(10893..11585)
                     /locus_tag="IVY70_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP7958871.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11607..12581)
                     /locus_tag="IVY70_001744"
     CDS             complement(11607..12581)
                     /locus_tag="IVY70_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7958872.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12881..13753
                     /locus_tag="IVY70_001745"
     CDS             12881..13753
                     /locus_tag="IVY70_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7958873.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14211..>15159)
                     /locus_tag="IVY70_001746"
     CDS             complement(14211..>15159)
                     /locus_tag="IVY70_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7958874.1"
                     /translation="RMLPIVLNEEMQATIYRMDKDDAEASKKVMEYCRLIQKRVKEYL
                     NLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIVPAAHEKTLIRY
                     PIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRLINELVQLGQLL
                     GVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITDHEFKNLSENIM
                     HIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHRLEVAKKWLKET
                     NLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
BASE COUNT         4487 a         3191 c         2485 g         4996 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttt ttttattgta tttgctgtaa
     2881 cagcattttg tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt
     2941 ataactcttt ttttcttgtt tttatattca caatgttggc tttttattgc attatcttga
     3001 cagataaaaa acaaaaaata atctaaaaac attgatgcat aagattgata acttttttta
     3061 cataacaata tatacaaaca aaaaaattca ctaactagtg aaaataagtt agtgaacctc
     3121 atttattaaa gaattaaatt acagatgatc ttttccatca tacttgaaaa actcaaaatc
     3181 agcataactt cctctttttt tatttagatc atgcactgct atcccgataa aatttccagt
     3241 aaaccctcct gagagtatac acatgttttt ttgcggacct actttcttcc attcttcagt
     3301 tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt acagtgactt tcaaaaaagt
     3361 cttcttttct gtgagtggaa tcgtccaatc ttctatttgt gctactccgt tttctgattc
     3421 tatcatacgt aggcattttc ccttgtcctc attccaagta acataacaat aaatataatt
     3481 ggattcattt aagtacagta ccagtccagc catctgatta aaattattag gcatgtattc
     3541 aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga atagctaaca aatgatgttc
     3601 gaaggtagat tgaagagatt caccggaaat cagacgtaaa aaacctggtc tacattttgt
     3661 atcacaccaa tcagcatttg gcaaaatgcg tctggcattc cagttcttct ttagctctcc
     3721 ttcaaatgta tccataaact gatgatcttt ttcttgttgt acctcttcat ttgtctcaat
     3781 cattatttct tccatcggag ttgtctggct atctttaagt cttaaccaat cgtcggacgt
     3841 ccaatacact tcttgaatag cggtttctct tcccaaaata gccgcaccgt ttaatggtct
     3901 ggtacaaaga tatgtcatgt accatcttcc tgacggggtt tgtactaagc tcccatgtcc
     3961 tgtacattgc aatgtagaag caggtttgtc actagctgtc agcataggaa aaccaggatc
     4021 aagctcgtaa ggaccagtaa tattctttgc tcggcaaact gtaactgaat gtcctcttcc
     4081 tgtaccgcct tctgccgtca tcaaataata atacccgtta cgaaaataca aatgtggtgc
     4141 ctccgttttc gctaattctg tacctgcaaa tattttatag atgggaccat caagcttctt
     4201 cttctctgca tcatattctt gtaatacgat accgctggat ttattcggtg tgtccagacg
     4261 ataatcccat agttcattga caagccattt ttttcccgtt tcaagatcat ggaacaaaga
     4321 aggatcaaag ccgttactat ttaaataaat tggttcggac caaggacctg tgatctcaga
     4381 tgcagtgatc aaataattgt ggacatcttt aaacggacgg cctgcacttt tgacatctgt
     4441 gtaaacaaga taaaacaatc catcagcata actgatctgt ggcgcccata tacttccatt
     4501 tgtaggattt ccttgcaaag atacttgagt ggtcaaaata tccgttgtat gtgaccagtt
     4561 gactaaattt ttcgactcat atactcggat tcctggaagc cattcaaaag aggagacaat
     4621 aatgtaatac cgctctttca ctcttaatat attaggatct ggattgaatc ctcttaaaat
     4681 aggatttttc actttgatag tctgcatgat tcaggctcct tttctcattc ttactcgata
     4741 taaactgaat gccccagctg cgaaataaaa aaatgcaaaa ggaaacgcaa taaactgagt
     4801 gccaatcaac aatatgaatc ctgttataaa atacaaacca gtggcagttg ttaagtattt
     4861 tcctttagca gtcaaaaaca atgctactgc taaaaaagca agtgaaataa acaacacaac
     4921 ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta ttttctaaag acaaagttga
     4981 atgatctaca gcaagcgact gataactttt tttaaactct gacgcagaca tattacttaa
     5041 agtaactgct aacccgccaa agaaaatcaa agatagactg ccaccgacca ttcctagcaa
     5101 tagttcccat tttcttttca tttctgaacc ccctgctttt ctaaatgtat ccattcaggt
     5161 cttttgatcg atgtttcata attgttatta cgttcagaga atttgacagt ctgttcattt
     5221 ttcttgtcat cccaattatc ccaaccagtt gggtgaacat gcccacctat cttacacgct
     5281 tcaaataaga catttgcata cggacgccac ggtcttccta aatagacgtt tctagtatca
     5341 ggttctgcag tcaaggtaca gctttcaaaa cgataccctg ttttttgatt tttcggcgta
     5401 gatgctgctg tgatgtatcc aggattttta ctggctaagc tatggatatg acactgatga
     5461 aaaacggcat ctgccccacc aaatatgaaa tccaccgttc cttcaatcca gcaatattca
     5521 taggtctgac gacaatactc aggattggca gaagccggtg tacaaaaagg agtaccatct
     5581 ttttgcaaat caggcaacgg acccgtgcat aaggtatcct gataaccaga tagtcgacag
     5641 ttttttaaag atacgcgatc tcctgaattg aataaagcaa cagcctgtcc taccttttct
     5701 cctggacctg ctgtattttc gatgtgaaga ttttctaata cgacattcga tccttctaag
     5761 aaaaaagtag cacttcggaa agtccctaac tggctaccgt caagatactc ctgttttgca
     5821 aaacgattac cggagataac tactgttcct gctcccacaa gtttaaaatt ggataatctg
     5881 ctttctatat attcatcata tcttccttct tttactatca agacctttgg atggctatcc
     5941 ggcaggcctg ccaaataatc gattccttct tggatagttt caaaatccgt cggggtatct
     6001 atagcattct ttccaattat cagtttgtct ttcatcctca taaatgctcc cttatgaatt
     6061 gtttccaaat atttctcatt tcttgtgttt ccatatgacc gcctgttaaa gaaaaagaag
     6121 aaaagttttc tggactaccc attgattgat actttttgtc cagccctttt ctcagcttca
     6181 gtacgccttc gtccaagcac atcgtatcgt tttttccaac taagctaaga cgagggcgcg
     6241 gagcgatttt ttcttggatt gctaacgttg tataagctgt caatagattt ggcacatagt
     6301 aataaaaccc atgatgatct aggcggcgat gtagcaaaag tatttcgatg tccacctgtg
     6361 ccgctagatc aatacaaact ttgacacgag aatccaatgc tgtcacccac cagcttaata
     6421 agcctcccat agaaagacca atcgttgcga accgttgagt atctgtgtct tcccttgtct
     6481 ccaaataatc gatcaagctg atcacatcat aaatacgcat tccccataac gtttttcctg
     6541 ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc tccgcgttct tcaaatcccc
     6601 aagcatcaat cgaccaaaca gcatatccca ttcctgtcag tacttctgca aaagatggag
     6661 aagctaagta aggagcacca tggatcaatt cacttttccc gactgaaaaa tctccgccgt
     6721 gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt ttctggataa gcatagaggg
     6781 ctggtactgt ttccaattca tttaagtaca attgatagta ttcaagcata aaaccgtttt
     6841 gtttttctcg gtataatcgt tttccactag gggtatgtct gctagatgga gcacctaaaa
     6901 aatcattcaa ctgcataagc aaagttttta cgaacaacca gttcatccat attttctgat
     6961 tgatttgatt ggaatttctc aatactgatt tctttagtat tttctaatag aaatgctggt
     7021 ccctcgtgat tttcaatcga catctggtta aaggaaacct tacccgcaaa gccaatataa
     7081 aatccttgtc tgctcatttc ctctattcct gtcatcatag caggaacacc gggaactgca
     7141 tcttgggcca tcgagacatc gatattgtca aatgtgattt cttgtatgaa ttgttccgct
     7201 aatccataaa taaaacctgc agaagcatgg acattccttg ccgtgatatt cgaaaaatgg
     7261 atccttctga atgctggtgt ccgttcatct atcggataag cctttttttc ccatacatac
     7321 ggttctttcc ctcgcggacc acagaaataa taaagattca gtataaatgg acacatcaca
     7381 ttatccatga ctatattgga tactcgaata tcttcaacga tcccgcctcg acctcgtcta
     7441 gatttcaatc gtattcctcg atcagtttct tggaagatac aatttgaaat ggtaatatta
     7501 cgaatactac cactcatttc acttcctaat acgacaccgc catgtccatg gaccatcgtg
     7561 caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc agtccctgcc
     7621 ttgattgcaa tacagtcatc tcccacgtca atatggcaat tactaatacg cacatttttg
     7681 catgattctg gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa gatcgtcaag
     7741 ttatcaaacg ttgcattact gcaaagaatc gggttgatcg tccaacttgg tgactggatc
     7801 aatttaatat ctttcacagt aattcgatga cagttatgga aactcatcaa tttaggacga
     7861 ggataagcga gattatctgg ttcatttcta aaagtatgcc accatttttt ccatttccat
     7921 ctaatgtacc gaaaccagtg acagagatgt tctctacatt ttgagcatag atacaggaag
     7981 cataaacttt tcgatggact ccttcccaac gtgaatgaac aacaggataa tcttttggat
     8041 cgtcagagaa tttcagaacc gctcctgcgc ttaaatgaag ttcgacattg ctttttaaaa
     8101 ataaagctcc tgttagaaat tcacctgctg gaacaactac ggtcccgcct ccgtcacttg
     8161 ctgcagcatc aatggcttgt tgtattgctt cagtattcaa ttcatcaatt gatgcgccaa
     8221 atgtcaaaat atcatacata tttataatgc ctccacttca atcaatgctt gcaagaatgc
     8281 accaacgcct ttttgatcat tacaaataat tggttcactg atgtagtaag catatgaacc
     8341 atctcgctga tctgctccgc ctaaccctgc tacttgacag tttttattca agttcagcca
     8401 gccttctttt gtttccagaa caaattcatc aagtaatcct tgatgtgatt tcttcacttg
     8461 tggaagccat tcttctttgt tcagcagtcc caaacgtatt cctttagcca ttgcgtaaac
     8521 aaacatacta ctgccggaag cttccagata atttcctttc cgttttgttt ctgttgttac
     8581 ttgataccat gtcccgctgt tttcttcttg atatggaagt aatgcagcca gtgtcttttc
     8641 tagaatttct ttcagcaaag aacaatctgt ttcttcatga agtaattcat aggtatctgc
     8701 tgcagccatc aaataccagc caatcgatcg gctccaaaaa tgatgggata gacctgtttt
     8761 tggatcagcc catggttgga ccttcttttc atcccatgca tgaatcagca aatttgtttt
     8821 tggatcgaat agatgtttat aactgatttc aaactgtctc aaaacatctt ggtaatcttt
     8881 ttcttcacta aaattctgga taaattcagc ataaaaagga gaacccatat ataagccatc
     8941 caaccaaatt tgataaggat aaatttcttt atgccaaaaa gctccttcgg atgtgcgtgg
     9001 atggtttgcc aactgctggg aaaggagttc tgctgctttt ttatattttt cttcgcctgt
     9061 ttctttgtac aataaaaaga aaagctttcc tgtatttaca tggtcgatat tgtattcttc
     9121 aacgctgtag ccacggatcg ttccatcttc ttgcacgaaa tagtcgatat tgtcttttat
     9181 gaaatcaaaa taaatttttt cttgggttcg ttcccaaagt aattggaacc ctttaagtac
     9241 gacgccatag tcataagacc attttccttt ataccacttt tcttcatata agcgaggtgt
     9301 tctttccatg atactttttg ctaatttctt tgaccagttt tccattattt gctctccttt
     9361 aaaaattaaa ataggatttg ggacaaatac caagaagtat ttgtcccaat cgtctatatt
     9421 actgtgccgc tgctttgtat gcttcctcgt actcttgtgt gattcgatcg cctccttgtg
     9481 ctttccagtc ttctacagct tttttgaatc catcttcgtc aagctgtccc atgatgtatt
     9541 gcaccgtagc atcaatgacg attttttcaa tttctgatcc tacttcattg ttcgtcggtg
     9601 aatccaatgg cacagaagga tccattacag caaattcttc attttctttg atcaattgat
     9661 tagagaattc tttttcaggg tctgtatctt tgaaagagaa cgtcacttca ctaggacgag
     9721 agcttgataa cggttgcacc tcttgttgcc aaagatccat atcgataaat tcgatagaac
     9781 catcatcatt caattcataa tgcgttcctt cgataccgcc agtcatcaat ttatacacat
     9841 cttcttctaa aagatcatca acaaattgaa gtaatttctt caattgtgct tcgtctttta
     9901 cttgagattt gggaaatgct agcaagccgc caataccatt tccttctgac caaacatgat
     9961 aaccgccgcc ttcaccatct gagatcttgt ttacaggcac taattccata tcatcttgaa
    10021 taccttgacc catcgtcttc agattcttga tatccaccat tccagtgtag attccagttc
    10081 ttccttgtgc aaattgttct tgctgatcag tcttttgggt aactgcaaag tcttgtgcca
    10141 aatagccatt ctcaaataga tctcttgaaa aattcaaagc tttcatatac tcttctgtat
    10201 caaattctgg agtgaatttg ccattatcgt ctacagccca gccatttggt gctccaaaat
    10261 aagaagtgaa aagtttaaag ctggtataac gaatatcact aggcgcacga tcgccaaaac
    10321 caactgtgtc gtccacacca tttccatcag gatcgttttc tgtaaattgt ttagctactt
    10381 cataaagctc atccatggta gttggaacat ctagtcctag attatccaac cagtcttttc
    10441 gaatgactaa gccagcacga gcgtagtctt tttgtatcgg tacgccgtac aaggcaccgt
    10501 cgatactcgc agcttcgatt ctatcttcag aaatttgact taaattttta tagtctttta
    10561 aatattctga tacatcccag aatacgcctg attttaacgc attgcggaca gaagagtttt
    10621 tcaacatcgc aatttgaagt gtaacgatat ctcctagttc atcagaagcc aatgcagttg
    10681 ttaatctttc ttcttttgaa gcatcaggaa tccagttgaa tgtcagttcc gtatcggttt
    10741 tttcttcgat caagttcaca atcgtgtctg taggcggtga agcatgatgc aaaatattta
    10801 accaagtgat tttagctttt ccatcttctg tctctgaaga actgttactt gaactgctgc
    10861 cgcatgcggc aagtgctaga attcctgcaa aactaagacc taattttacg agcgttgctt
    10921 ttttcataaa ttttctcctt cactgtttta ataatatttt tgttttcttc tgttttttct
    10981 gtatatgagt tcttccattc caaatggcaa aatatagaat ttgctgtcca cgtgactgcg
    11041 gaaaagaaga gactgccacc gataaaggtt aaaacacctg gcattgtttg gatgatcatc
    11101 agataaatcc ctaccatact taatatcaac actaatgagt aatgaagatg tgagaatccg
    11161 aataaaaatg ccattttcat tttgaaaaaa tatcctttcc aatcataatg agccatgatt
    11221 gggaaaatat agagtgctga gagcacatat atgaacgcag caatatacaa agcgatccta
    11281 agataccaat tgtgtatgaa agaaagatcc acaagtaaaa caatcccgat ggcgccatat
    11341 atccagctca tcagcaaggt ttcacgatag ttttctttaa aatatgtgaa gtacgttttc
    11401 gtcaaaggaa acgtttcttt tgagcgcagc cattttctaa tcactgcaac catcgcatac
    11461 gatgcaggac cgaaagtaaa aactcccaaa cctgcgatga ctaaaccgcc ccacagaaaa
    11521 ttcaaataaa ccaactgtaa aatacgattt agccttccgt ttattttatc taatgactgc
    11581 agcatatctt tccctctctt tctatcttag taaacgccgt cttctcctag ctttttggca
    11641 atcttattag ctaaaattac cataaacaat cctacgaccc ctttaaacaa tcccattgct
    11701 gtactgaaac ttaactgtcc attcttcaat ccagctgtat aaatataggt gtcgaaaatt
    11761 tctgcaacgc ttcgattcaa tgaattcaat aacaagtaca tatgctcgaa ccctaagtct
    11821 aatgtctgac cgatttttag aatcagcaag gtaatgatta ctggacgaat tgccggcaat
    11881 gtaacatgcc atgttttgcg taagcgtcct gctccatcca tttctgcagc ttcatacaac
    11941 tgtgtatcga ctgcagttat cgctgaaagg tagatgatcg tagaccagcc caactctttc
    12001 cagatcactt gtccgatata tactgtgcgt gtccattcag gggatgttag aaaactgatt
    12061 ttttcaaatc ccattgttgc aaggaattca ttcaataccc ctccgtcaac attcaaaaat
    12121 acataagtaa tcgaaacaat gattacccaa gacatgaagt gagggatgta aatgatcgtt
    12181 tgaatcccgt ttttcaatcg tttgttcgtt acttcattta atagtaaaga caaaatgatt
    12241 ggcatcggaa aaaacacgaa gatatttaat ccgaacaaga tcaacgtatt tcttaataat
    12301 gtaaagaatg tcggttctgt aaacaaacga atgaaatgtt tgaaaccgac ccaaggactt
    12361 cccattattc ctaaaaatgg ttgataatct tgaaaagcaa taacgagtcc ccccatagga
    12421 agatacctaa aaatcaagaa ataaaggact cccggcagaa tcatcacata taaaaattta
    12481 ttcgttttga ttcctgcaat tatttttttt cgtcttctga gcttttcttc ttttttcgat
    12541 agcgtcagtt gaaatggttc ttgtttcttt acactttcca taagcccctc cttagttttg
    12601 tgaatgtttg ctgcttatga cttcatgata gataactatc tgaattacgt atataatcat
    12661 ttttaccgta agtaaaaacg cttacatagc aatgattttt gtgctataca aaacgataaa
    12721 gtgtattttt tcacaaataa actacgcata gcaaaccttc tatttttttc tatctgacac
    12781 ttagcatagg aaatgattat atacgtaatc tttcaccgat gataaattga acatatagaa
    12841 acagaaacat ttaatttatt ttgaggagga gcctatctat atgaaaaaga aagtctctat
    12901 aggagaaaga agttttacac ttttcaacaa tacgtttcta attttgctag ctttgatctg
    12961 tatcgttcct tttttaaata tcatcgctac atcttttgct tctacacaag aagtcgtcgc
    13021 gaaaaagttt atcctttttc ctactacctt ttctttagac gcgtatcgct acattctttc
    13081 tacgccgacc atttttagag cacttgctgt ttcgattggt gttacaggtg tcggaacgat
    13141 cgtcagtatg tgtgcaactt cactgatggc ctacggtctt tccagaaagt atttgtttgg
    13201 tcgaggtttc gtcaactttc ttgttgtatt ttctatgttg ttcagcgggg ggatgattcc
    13261 aacgttctta gttgttcgtt ctctagggct ggtcaattct tactgggcca tgatcctgcc
    13321 tgtggctgtt aatgcaatga atatgatcat tatgagaaac tttttccaag ccttgccgga
    13381 tagtttggaa gaatcagcaa aaatggatgg ttgcacagat tttggtgtat ttttcaaaat
    13441 tatgctgcca ttagcgttac cgtctattgc tactatctcg cttttttatg ctgttactta
    13501 ctggaacact tatatgactg ctattttgta tatcaacgat tcatctaaat ggccgataca
    13561 gattctttta cgccaaatcg tcattgtatc aagtgggatg caagcagaaa gtagtgccgt
    13621 tgatgtgatt ccaccagcac aaacagttaa aatggctgtt atcgttattg caacagtgcc
    13681 tatgctgatt gtttatcctt ttgttcagaa atattttgtc aaaggagcac tcgtaggttc
    13741 tgtcaaaggg tgattatgta ctgaacgcaa aatggtagtt gtgtcagaag tggtcagctt
    13801 caagaaataa gaaggcattt acgaaaattg cttcttaaat ttttgtgaaa tttcggctta
    13861 tttttcgaag aatctactcc tggaacaccg tgtatcagag gttgtgaaag agacgttcag
    13921 ccttgagcga tgataaaaac tctcacaaac attgcgacga gtaacccaca ggagcaataa
    13981 gaagcgattt tgaaaaatag cttcccatat ttcacaaaat tatgccttat tgtcgatagc
    14041 ctgtctcttg aacgccattt attcgtaaaa aagaactgtg acaagacttt tgtcacagtt
    14101 ctttttgcgt gcttagcctt gaaatttaac tatccataaa tacaaatact agggttattc
    14161 taacttcttt tattgcttga atcagtccac aagaaaaaca gcctcttgat tcacaaatat
    14221 ttttttcgat attctcccgg cgtaatcgat tccttctttt taaagaaacg aataaaattt
    14281 tgcggatttc gatattgcaa tctttctgca atctctttta cggagaggtt agtctctttc
    14341 aaccattttt ttgctacctc taaacgatga ttttgaacaa aatcaccaaa tttttctcct
    14401 gtttcctttt taaatacatt acttaaatag ttagggttat aatgcaacct atcagcgatc
    14461 acatctaaag acagatcacg atcatattct gtgtagacga tatgcataat attctccgac
    14521 aaatttttaa attcatgatc agtgattgct tgagagcatt ctgtgattgg cagaactaat
    14581 tgttctaaaa caagtttttc tagttctttg ggatgataat tgttcagtgc ttttacataa
    14641 atttgtttca tgttatcaaa gttttttgaa tctactccca ataactgtcc taattgaacg
    14701 agttcattga tcaagcggac cgaaactact tcccgactca aaggattttt attttgtgca
    14761 aacaatgcat caatcaattc atgtactaaa ttcttcactt cctgatttcc agaacgaatg
    14821 gcttcaaaca atcgattttg ttcttctatc ggatatctaa taagtgtttt ttcatgagca
    14881 gcaggaacga tttcctgata aaaaataatt gaacttggac cagtatttac acgataatac
    14941 agggcttctt ttgcccgatc aaccgattgc ttgctttcat tcagcgtttc gaaccggtta
    15001 ctgatgccta tactgatagt taaattcaag tattctttta cacgtttttg aatgagtcgg
    15061 caatactcca tgactttttt gcttgcttct gcatcatctt tatccattcg ataaatagtg
    15121 gcttgcattt cttcattcaa aacgatcggc agcattcga
//
LOCUS       DACCXO010000061        14970 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.062, whole genome shotgun sequence.
ACCESSION   DACCXO010000061 DACCXO010000000
VERSION     DACCXO010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.062"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..626)
                     /locus_tag="IVY70_001747"
     CDS             complement(<1..626)
                     /locus_tag="IVY70_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7958875.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
     gene            899..3370
                     /locus_tag="IVY70_001748"
     CDS             899..3370
                     /locus_tag="IVY70_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7958876.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3473..5209)
                     /locus_tag="IVY70_001749"
     CDS             complement(3473..5209)
                     /locus_tag="IVY70_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP7958877.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5206..5910)
                     /locus_tag="IVY70_001750"
     CDS             complement(5206..5910)
                     /locus_tag="IVY70_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7958878.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6041..6544
                     /locus_tag="IVY70_001751"
     CDS             6041..6544
                     /locus_tag="IVY70_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP7958879.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6504..6842
                     /locus_tag="IVY70_001752"
     CDS             6504..6842
                     /locus_tag="IVY70_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP7958880.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6863..8281
                     /gene="ffh"
                     /locus_tag="IVY70_001753"
     CDS             6863..8281
                     /gene="ffh"
                     /locus_tag="IVY70_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP7958881.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8393..8971
                     /locus_tag="IVY70_001754"
     CDS             8393..8971
                     /locus_tag="IVY70_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP7958882.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8975..9496
                     /locus_tag="IVY70_001755"
     CDS             8975..9496
                     /locus_tag="IVY70_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7958883.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9575..10129)
                     /locus_tag="IVY70_001756"
     CDS             complement(9575..10129)
                     /locus_tag="IVY70_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP7958884.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10382..10657
                     /gene="rpsP"
                     /locus_tag="IVY70_001757"
     CDS             10382..10657
                     /gene="rpsP"
                     /locus_tag="IVY70_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP7958885.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10669..10920
                     /locus_tag="IVY70_001758"
     CDS             10669..10920
                     /locus_tag="IVY70_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP7958886.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            11045..11836
                     /locus_tag="IVY70_001759"
     CDS             11045..11836
                     /locus_tag="IVY70_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958887.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            12006..12527
                     /gene="rimM"
                     /locus_tag="IVY70_001760"
     CDS             12006..12527
                     /gene="rimM"
                     /locus_tag="IVY70_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP7958888.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12517..13278
                     /gene="trmD"
                     /locus_tag="IVY70_001761"
     CDS             12517..13278
                     /gene="trmD"
                     /locus_tag="IVY70_001761"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP7958889.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13414..13761
                     /gene="rplS"
                     /locus_tag="IVY70_001762"
     CDS             13414..13761
                     /gene="rplS"
                     /locus_tag="IVY70_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP7958890.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            14019..14312
                     /locus_tag="IVY70_001763"
     CDS             14019..14312
                     /locus_tag="IVY70_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP7958891.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(14400..14513)
                     /locus_tag="IVY70_001764"
     CDS             complement(14400..14513)
                     /locus_tag="IVY70_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX protease"
                     /protein_id="HAP7958892.1"
                     /translation="MYAPGTILLTIAYLTANRSLAFIIAIHILNNVLGFVL"
BASE COUNT         4976 a         2711 c         3040 g         4243 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gattggttgt cccatcaaca caaataaaac tcatggaacc agaaacagaa
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      361 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      421 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      481 tgttcacaat aggaagaaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccctttt tttttgctat
      781 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
      841 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
      901 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
      961 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
     1021 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
     1081 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     1141 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     1201 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     1261 tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct
     1321 tgtaatagat gacattgtta ttttagagac tggatatcaa attccagcag atgcagaggt
     1381 cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     1441 caaagaaata ggtgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     1501 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     1561 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     1621 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     1681 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     1741 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     1801 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     1861 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaaa tgatcgtctc
     1921 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     1981 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     2041 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     2101 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     2161 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     2221 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     2281 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     2341 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     2401 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     2461 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     2521 aaagaaaatc ttcgttcaat tgataaagcg taacaataca gtagctatga caggtgatgg
     2581 cgtgaatgat attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa
     2641 tgaagccgcc atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc
     2701 agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt
     2761 tttagtgaag aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac
     2821 ctatccttta gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc
     2881 tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt
     2941 gatggaaaaa gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat
     3001 ctgcggggtg actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt
     3061 gattgcagta gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag
     3121 tcagatcatt cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct
     3181 gttttcaatc accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc
     3241 ggcagattcg atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa
     3301 tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg
     3361 agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt
     3421 tcgaagcagg atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt
     3481 tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac
     3541 cattaaggag ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat
     3601 gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa
     3661 tgtcgatacc tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc
     3721 ggataataat ctcgctgtcg ctttttgaat attggacagc attttcaatc agatttttga
     3781 taattggata aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa
     3841 tcgtcaaccg tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag
     3901 gttctgtata aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga
     3961 tttcccgaat cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag
     4021 tcagagtttg cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg
     4081 aagtcacagg agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt
     4141 ctaaacgtcg cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg
     4201 aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct
     4261 cctgatggat cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct
     4321 cttgaatgac ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct
     4381 gcatctttgg attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa
     4441 tcatcagttc gttcagtaac gtatataatt gctcttctgt ggtagtacac gcccgataca
     4501 tcttactcat ttgttcgctc aacgcattaa tcgtctgata caattcttcc cattgatcgg
     4561 gtgtttgcat gattacttcc gcctgattag gattttgcac catttttttt agtacgggca
     4621 gcacggtttt caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa
     4681 caaggaagaa agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta
     4741 aaaaaacgct tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga
     4801 ctggtaatgc gacatataaa agatcctcat tcaaagtagt acttttgcgc aaagcgctac
     4861 ctaggttccc accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca
     4921 atgtttcatt gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt
     4981 cgttcgactg atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt
     5041 cgacatctaa ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt
     5101 ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa
     5161 ctaaaagaat aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc
     5221 ctgaaactta tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg
     5281 ctcgattttt tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa
     5341 gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt
     5401 catgaaataa acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac
     5461 ggtcacttga taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc
     5521 agcttcatct tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc
     5581 taatacttcg cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc
     5641 aatgatccga tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt
     5701 ttcttgacgc agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa
     5761 aatgatgaaa tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc
     5821 actggttacc ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg
     5881 ttcatcatca acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg
     5941 tttatatttt agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata
     6001 ataggaaggc actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg
     6061 tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga
     6121 actagttgaa actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat
     6181 tcctagagat cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc
     6241 aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa
     6301 agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc
     6361 ttcatgcgga acccattcga tttatgacgg aagtttttcc ggaataaaaa aaaatggtgc
     6421 cggagtggct actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg
     6481 gctgaaaact agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct
     6541 ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg
     6601 cagatgattt ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat
     6661 atgacaatat caagcggaca agcaagatct tggaggatta tgaaaaaaaa ctccatcttt
     6721 tttctaatta tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata
     6781 agtacccgga agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat
     6841 aaataaaagg aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc
     6901 gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg
     6961 ggaaatccgc cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac
     7021 taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca
     7081 acaaatcgta aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga
     7141 gttaaataaa tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg
     7201 taaaacgacc tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc
     7261 gctcctaatc gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg
     7321 tcaacagcta gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt
     7381 ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc
     7441 aggacgtctg catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac
     7501 gcaaccaaat gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt
     7561 agctgatagc ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg
     7621 ggatactcga ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt
     7681 catcggttct ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag
     7741 ccgtatcctt ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactacga
     7801 cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga
     7861 ctttattgaa cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat
     7921 gatcccagga atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt
     7981 tgaacgcaaa aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct
     8041 gttgaaccca agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt
     8101 gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg
     8161 ggatatgaac atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg
     8221 caaaatggcg atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata
     8281 agatgaaaaa aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca
     8341 gcaattttta ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg
     8401 tccgtggagt gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc
     8461 tgaatatgat gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt
     8521 gagctggcag acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga
     8581 tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg
     8641 aattattcgt aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa
     8701 aattcaagaa gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat
     8761 cgttgattct tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga
     8821 attagcaaaa gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc
     8881 ttttttagaa gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga
     8941 ggtcgtacgt atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc
     9001 tataagttta gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac
     9061 tccaacaggt aagcaaacta cttatgaagc tttttatgaa acaatcgata cgaccatcat
     9121 gggcagaaaa acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga
     9181 gaaaaagaat ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt
     9241 aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg
     9301 ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg
     9361 gtatatccag attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac
     9421 atatgaagcg cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca
     9481 ttatgtacga aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg
     9541 tacatagttg ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat
     9601 ctagatacgc atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat
     9661 tttcatcaat tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca
     9721 cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc gccaaagccg ccgatcccac
     9781 ctggtgatgc agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc
     9841 ttgagcctac atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag
     9901 taacgaaaac gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag
     9961 gagcctgttc tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa
    10021 atacactttc gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt
    10081 aagaatcttt tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc
    10141 tccttcaaac tatctataat aatcttacaa aagataagta actggagcaa ataaaaacac
    10201 ttactgacaa aagctacata gaagatagat tacttatttt taacaaaaaa gaagtctaaa
    10261 atcttctatt ttctaaaaaa aatcgaattt aatcaagctg taaagaaaaa aagctttaca
    10321 gggtaaaaaa aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat
    10381 aatggcagtt aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat
    10441 cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta
    10501 caatcctttg aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt
    10561 atcaaaaggt gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat
    10621 gcaaaaacac cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta
    10681 agcgacttag tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta
    10741 gacgtcgtag agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt
    10801 ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt
    10861 gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa
    10921 ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt gtttttttta ttaattgaag
    10981 aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca
    11041 gccaatgaga aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc
    11101 ggttactcaa gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag
    11161 tccagaaaaa agtagtacca aaacggcttc caccactgaa caaacaatgg ctacctcaaa
    11221 taatgagaaa aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc
    11281 tctagatgta ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg
    11341 ttattctatt ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa
    11401 tcaagaaacg cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac
    11461 aatccaagtt ttgcagccat ttgagatcga cacaaatggt cagcaagtgg acttaggttc
    11521 tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg
    11581 aaactggcgt attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc
    11641 taaagaaatc gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa
    11701 aattactgtt gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc
    11761 gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc
    11821 tatgaagagg ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca
    11881 ggaagtaatc aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag
    11941 aaaactagaa ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat
    12001 ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg
    12061 aagtccgtgt tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc
    12121 taacactatt ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac
    12181 ataaaaattt tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat
    12241 atcgggatgg catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat
    12301 tctattacca tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag
    12361 taaaagaaat cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag
    12421 ctgatatatt gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag
    12481 tggtccgtgt agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta
    12541 tttccaagaa tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa
    12601 ggattattag atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca
    12661 gtagatgatt atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat
    12721 gataacatcc aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt
    12781 gacccagctg gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac
    12841 ttagtattca tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca
    12901 gatgaagttt ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg
    12961 atcgatgcaa cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg
    13021 gattcttatt caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa
    13081 gggatgaaag taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa
    13141 ttaaaagaat cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa
    13201 tggacacctc aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca
    13261 gcagatgaag ccaagtgact ttttctaaaa tcttctttac agtaggttga taatatgtta
    13321 gtattttatg gtgagtgcaa aagcacttaa ctattacgat attccgctgt gagaaaagct
    13381 cataagaata tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca
    13441 aaagaacaat tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac
    13501 gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa
    13561 cgccgcggtg ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc
    13621 gttgaacgta cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac
    13681 ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt
    13741 atcaaagaaa tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa
    13801 gggttctttt ttcttagcat gtaatttgaa caccctgatc cagttttttc tttaattatt
    13861 ggtaggggag aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc
    13921 actatgataa tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac
    13981 ataaatggag tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc
    14041 aagaaaagat gaaagtttta gacacattca tgaatgatgg aaaagtaaaa caagtaccaa
    14101 gttcaacaaa aaagaaatac atcctattga aagaagtctt gaaacgcttt gaccatggag
    14161 tgacttatac agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt
    14221 atgtcgaaca aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc
    14281 gggcatatca gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa
    14341 gttcaagtat gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc
    14401 tataatacaa agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag
    14461 cgattcgcag ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa
    14521 tcaattggat gcgttacatg aataaagata attctctttt atttgtacga ataaatacca
    14581 aaacgtacct cctaaaatta gattttggac agatgccctt taaaaagata acaagatttc
    14641 gttcttgaaa caatatatat gcttttattc caatgaggtt gtgaaaacag tattttgtcc
    14701 tgagcgttag aaaattgaaa atggggaata gcaactcgta ctttaccatt ttttaagtga
    14761 atacaagatc agctaattga acaccgttta ttcgtgatga gggacacaac actgcagtcg
    14821 acacatttct cttttatgtt caaagataac aaatatttaa aacaaagaaa aatagtagat
    14881 aatcccgtcc taactctttt attcttccct catagcttcc aaatttaagc aatacttcaa
    14941 ttaatcaagt tttatctttt tttttcaata
//
LOCUS       DACCXO010000062        14883 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.063, whole genome shotgun sequence.
ACCESSION   DACCXO010000062 DACCXO010000000
VERSION     DACCXO010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.063"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(6..335)
                     /locus_tag="IVY70_001765"
     CDS             complement(6..335)
                     /locus_tag="IVY70_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP7958893.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            512..1240
                     /gene="sfsA"
                     /locus_tag="IVY70_001766"
     CDS             512..1240
                     /gene="sfsA"
                     /locus_tag="IVY70_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP7958894.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            1256..2269
                     /locus_tag="IVY70_001767"
     CDS             1256..2269
                     /locus_tag="IVY70_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7958895.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALIDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            2269..3546
                     /locus_tag="IVY70_001768"
     CDS             2269..3546
                     /locus_tag="IVY70_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP7958896.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            3609..6311
                     /locus_tag="IVY70_001769"
     CDS             3609..6311
                     /locus_tag="IVY70_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7958897.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPECLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            6463..6780
                     /locus_tag="IVY70_001770"
     CDS             6463..6780
                     /locus_tag="IVY70_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7958898.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            6810..7130
                     /locus_tag="IVY70_001771"
     CDS             6810..7130
                     /locus_tag="IVY70_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7958899.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            7238..8698
                     /locus_tag="IVY70_001772"
     CDS             7238..8698
                     /locus_tag="IVY70_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7958900.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            8766..8987
                     /locus_tag="IVY70_001773"
     CDS             8766..8987
                     /locus_tag="IVY70_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958901.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            9018..9200
                     /locus_tag="IVY70_001774"
     CDS             9018..9200
                     /locus_tag="IVY70_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP7958902.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            9200..9613
                     /locus_tag="IVY70_001775"
     CDS             9200..9613
                     /locus_tag="IVY70_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP7958903.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            9736..10917
                     /locus_tag="IVY70_001776"
     CDS             9736..10917
                     /locus_tag="IVY70_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7958904.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            11306..11379
                     /locus_tag="IVY70_001777"
     tRNA            11306..11379
                     /locus_tag="IVY70_001777"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11340..11342,aa:Arg,seq:cct)
     gene            complement(11448..12587)
                     /locus_tag="IVY70_001778"
     CDS             complement(11448..12587)
                     /locus_tag="IVY70_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7958905.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(12886..13521)
                     /locus_tag="IVY70_001779"
     CDS             complement(12886..13521)
                     /locus_tag="IVY70_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958906.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(13634..14269)
                     /locus_tag="IVY70_001780"
     CDS             complement(13634..14269)
                     /locus_tag="IVY70_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAP7958907.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(14303..14764)
                     /locus_tag="IVY70_001781"
     CDS             complement(14303..14764)
                     /locus_tag="IVY70_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAP7958908.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
BASE COUNT         4741 a         2507 c         2920 g         4715 t
ORIGIN      
        1 gggatctatt tttcagataa caacttatat gaagtgtctg cagattcgtc ataaagagtt
       61 tgttcgggat aagaaaagat aagataaaaa ccatactgat cacgtacgac taaacgatat
      121 tcatctattt ctaccacaga tgtgccaaga gactgaccgt tgatcaaaaa cgtgtaatca
      181 gatgtaatct gtaattgttg tttttgctgg ctatcactaa agaaccatgt accttgatac
      241 tttgagagtc cttctgactt ttccgcttgt tttttctttt tcaagtaaat aaaacttgag
      301 agaacggtaa tgagtgaacc aagaaaatag atcatttttt ttatcaccaa atcactcctt
      361 tatgagaaaa taatcgcata gacatattct ttcttcttta tttcattgta ccgaataaaa
      421 gctaaaattt ctctaagact ttcgactggt ttttttactg tttttctaca aattatgata
      481 ggatacatag taggaagaaa ggtgacataa tttgaagtat cagcaagtgg aaatagcttt
      541 ttttattgac cgcccgaatc ggtttatagc acattgtatg aaaaaagacg gagaaataat
      601 tattgcgcat gtcaaaaata caggtagagg aaaagaggtc ctgataccag gcgctgaagt
      661 ggctattgta tttgctcctg gacctaaacg gaaaaccgcc tatgatctga ttgctgtaaa
      721 aaagaagggt gactggttta atatcgatag tcaattgcct aatcggctgg caattgatgg
      781 tattttagac ggcacgatcc ttttaccagg attaactggt ccatttacgg tatacaagcg
      841 tgaagtaact tatcttcact ccaaatttga tatttattta gagacggtgg tcggacaaaa
      901 agcatttgtt gaagtcaaag gtatgacact tgaaaataaa gcgattggtg cttttcctga
      961 tgcgcccaca ctcagaggat tgaaacatat aggagaatta acgtatgccg cacaagatgg
     1021 ttatgccgct tatgtcttgt ttattgctca gttcgaacat cttcatctgg caacgatcca
     1081 tgaagaaatg caaccagcac tagctgatat ggttcgacat gcgcagcaaa gcggagttca
     1141 aatcctcgcc tataattgtc aagtatcggc agatgaggta actgtcaagc aggccattcc
     1201 atttgatgta gatatccctt ttgaagatcc tatacaatag aaagaaggta aaataatgat
     1261 caacttaggt attatcggaa caaattggat tactcatcaa tttgtccaag cagctttaag
     1321 cacaaaaaaa tatgaattga cagcggtata ttctagacat ttggaaaaag cacaggaatt
     1381 tggttctcat tatgaagggg atatcgaatt cgcaacagat ttgaagactt ttttcaatat
     1441 tactcatatg gacaccattt atattgcttc tccgaatagt cttcacttta gtcaagctaa
     1501 gcaagcaatt ttggcaggaa aaaatgtgat agttgaaaag ccagcttttt cgacgccaaa
     1561 agaaatggat gaaatcatcc atttggcaaa tgaacacaat gtgtattttt ttgaagcagc
     1621 aagaaatatc catgaatcag gttttaaaaa gattgccgat tacttaccat taaaagacga
     1681 gataatcggt gcaaatttca gctatatgaa atattcttcg cgctatgacc aagtattaga
     1741 tggagaagaa ccgaatatct tctcgactca tttctcaggt ggtgcattga tagatttagg
     1801 tgtttacctt gtatatgcag ctgttgcttg gtttggaatg ccaaaagaag tccattattt
     1861 cccaagaaag atttctactg gtgtagatgg tcttggatgg gggatcttac gttatgagaa
     1921 ttttgatgta tcgatccagc caggtaaaat cggtgattct tatttaccgt cagagattta
     1981 ttttgactca ggaacgctag tgatgaatgg agtcaatgca attgaacaag cacaatttca
     2041 tgatcgcgcg cataaagaaa tcattgattt ggcggtaaca gctgcagaaa atccaatggc
     2101 agatgaagca gaggattttg ccaatgtcat ggctcatccg aaagatccag cttggggatt
     2161 gttgtatgaa gagtggatcg agctagctag aaatgtgaac caagtgattt atgatttaag
     2221 aaaaaatggc ggcatcaaat tcgatgcaga taatgaggaa gatttttaat gaacgaattt
     2281 gtacaagtat ttcccgaaaa ttggcagaaa aaatatgagg ataaagggtt tatctcacct
     2341 tctcttattc aacgagcagt ttttcagcca ctaagtaata aacaaagtat agtagcggtt
     2401 tctcctacag gttcaggtaa aacactggct tatctttggc ctctactact aaacgtggaa
     2461 ccaggtgaag caagtgcgtt ggtcattttt gcttcttctc aagaactagc tattcaagta
     2521 gcagatgttg cacgtgaatg gggaaaagac aaagagttga aagtccaatc tttggtgggc
     2581 ggagccaacg tcaaacgtca gattgagggt ttgaaggaaa aaccagaaat cttgattggt
     2641 acaccaggaa gaatattgga attgatgaaa gcaaaaaaaa taaaagcaca tcaagtgaaa
     2701 acaatggtat ttgatgaagc agaccagcta tttgacgctg ggaacagcca aatcattgat
     2761 caaattcttc atcaagctcc aacagagtat cagctagctt ttttttcagc gacagcagat
     2821 cgttcccttg aaagcattga aaaaatcact gggaaaacgt ttgagacaat tgacgtgaca
     2881 gcagaggacg attctcgaaa aggactgaga cattactttt tacgtgtacc gatacgaaag
     2941 aaagatgact acttgagacg tctcacgcat atagaagatt ttcatggtct gattttcttt
     3001 aatcaactga ctgaattagg cgtaatggaa gaaaaattgc tgtatagagg tgtaccggta
     3061 ggaagtctgg cctctgatca gaacaagcta ctccgaaaag cagcgctaga acaattcaaa
     3121 aagaaaatgg tcagagcact tttgaccaca gatgtggcgg ctagaggttt agatattaca
     3181 ggtcttcctt atgtagtgaa tgcagaagtt ccgttaagtg aagaagctta tttgcatcgt
     3241 tcgggaagaa ctggtcgaat gggtaatgaa ggaatagtaa ttacactcgt acaagataat
     3301 aatctaagag atttaaaacg gcttttacgt ccatcaaata taacacttga agagattttt
     3361 ttatacggag gtaagctgca aactgagcag ccaaccaaag aagatgcagc tcctgcagta
     3421 aataaaaagt atgcgaaaac tttttctgga aaggagcaga acccagaata taacaaagaa
     3481 aaaataagaa aaaaagttaa aaataaatca aaaaaagaga gaaataaagg agcaaggcgc
     3541 aaataagcgc ctttttatgc tatgattatg aaaacgggaa caaaattggt tgccagagga
     3601 ggggttatgt gaaagaacgt caagaagaga tcttgcagct attgacagaa aaccaacaag
     3661 gattgacagc aagtgatgta gctgagcgct taactatcga tcgaagcaat gcaagccgat
     3721 atttaagtga actatacaag gcccatcata ttgtaaaaac cgctggccga ccagtcgttt
     3781 actctttgcc aacggaaaag tcaaaatctg atgaagtgca tgtcgactct tctacgcaag
     3841 tgacttttga aacactcgtt ggagaaaatg attctttaaa agtcagcata cagcaggcaa
     3901 aagctgcgat tctttatcca ccgcgggggt tgcatacgat tatttttgga gaaactggta
     3961 cagggaagtc gatgtttgct gaatgtatgt atcattttgc aatcgattca gagatgcttt
     4021 ctgcagatgc gccatttgtt tctttcaatt gtgcggatta tgctcaaaat ccgcaactgc
     4081 tctttggaca catctttggg atcaaaaaag gggcttacac aggagcagct caagatagtc
     4141 ccggtctaat agccaaagca gacggcggta tattgttttt agatgagatc caccgtttac
     4201 cgccggaagg gcaggaaatg ctgtttactt tcattgataa aggtatttac cgtccgcttg
     4261 gagaaagtgc acaagtacat gaagcatctg ttcagatcat tggggcgacg actgaatctt
     4321 cagaaagttt cttatctacg ttcaatcgac ggattcctat ggcaatcacc ttgccgaatc
     4381 ttgcttcacg ttcactggat gagcgttatg aaatcatctc tctttttatc aaacaagaag
     4441 cgaatcgttt gaatcaacgt atcgatatcg aaaaagaagc gattcttgcc ttcatgcttt
     4501 atgatgcgga aggaaatatc ggtcaagtga aacgagactt gaaactagtc tgtgcaaaat
     4561 cttttttaca ttaccgtact catcatgaga aaaagctaat cattcgaaaa gaagaacttt
     4621 cccttcaagt acaaaaagga ttactgaaaa tcaaggaaat accggaatgt ctcgaccgat
     4681 tcatggacag caaaagtcag tatttgacat ttgaaccagg atttgctgat gttgtctggt
     4741 cacaagatcc agaacgaaac atgcaggtct ataacgatat tgaggaaaaa gtcacttctc
     4801 tttccgaaac cggtgtggag aacattgatc tagagaccct catttcaaaa gatgtggatg
     4861 cttatttcca gacatatgtc aaagaattga ctaaaagttc gattcccaaa gatcttttgc
     4921 cagatgaaat ctggcggtta accaatcaat tgtatgatgt ggctgaaaaa cggttagacc
     4981 gtatttataa tgaaaaagct cgttttgctt ttgctttgca tctgcaaagt acattggatc
     5041 gtgtgagaga aggtcatatg atcgtccacc cagatttgaa taacgtacgg aaaaatttta
     5101 agagggaatt ccaggtagca atcgatcttt caacgatcat tgaagaagaa gaacaagttg
     5161 agattccttt tgatgaaatc ggctttatta gtatgtttct ttctatcaat ctaggagaaa
     5221 gtgagcctgt taaagaaaac aaagtagatg ttctcatatt gatgcacggc agacagactg
     5281 cttcaagcat gttggagatg gcacaagaac tattaggaac aaaagtaggg aaagctttta
     5341 atatgccact ggttatggaa gttcaaacaa tgtatgaaga agtgttacaa tatgtcaaaa
     5401 actatcaaga gcatttgacg aatggcctac tcttgttaac agatatggga tcattaaaca
     5461 ccttcagtga tttgatttat gaagagactg gcattcggac gaaagctatc acgatgacaa
     5521 gtaccatgat tgtgatagaa gcgatacgga tggcaagtgt tggaagaagc ttagaagata
     5581 tttatcaaaa tatccagctt tcctttgaat cgatcgtacg tgatcagttc aaagcggaaa
     5641 agtctgaaac tttacagcgt aaaaaagccg taatcgtgac atgctttact ggtgaaggag
     5701 tagctgccaa attatatcag cgtattatgc cagtgattaa tcaaaataag gtagaactga
     5761 tccagatgca gtttttagaa cgtgaatcat tcaaaaaaca catcgatgcc ttattggaag
     5821 aatatgagat caaagcaatt gcgggaacag ttgatgttga ttatcaaaat atcccatttt
     5881 tttcagccta tgacgttttt gatgatgaaa aactaaatat tttaaagcgt atagctgatg
     5941 atgagatccc gacagctaaa attgtcgatt cacttgctgg atcattaagc gatgtctttt
     6001 ctattgatga actgatccgt tcattgcaaa agacagttca gcagatccag accgatttgt
     6061 cgattatcgt ggaacctgga gtagatactg gaatcgttat ccatttagcc ttcatgttag
     6121 acggattgat caaacaagaa agccatcggc aatttgagaa actgtctatt ttctcaaaaa
     6181 tttatcgttt ggaaatggat atcgttcggg caaacttgat ggcactagaa cgaaaatatc
     6241 aggtaaaaat tccagaagat gatatcgcat ttcttacaca aatgtttctt gaaaataaaa
     6301 taaaaaaatg aacacactaa tcatactgtt tacacactaa acatctgtgt gcgcactata
     6361 aaaaagagct aaaaagcatg aaaacgctat ttcagctctt ttttttattg gcatgcttct
     6421 tgcgttatat aaaagtgtaa aaaaataaaa ggagtgttaa aaatggcaaa aaaaacaatc
     6481 atgttagttt gttcagcagg gatgagtacg agtttattgg tcacaaaaat gcaaaaagca
     6541 gcagaagaaa aagggatgga agccgatatt tttgcggtct ctgcctcaga tgcagataat
     6601 aatttagaat ctaagaatgt cgatgtatta ttattaggtc ctcaagtacg tttcatgaaa
     6661 gctcaatttg aacaaaaact tgcgcctaaa ggtattccat tagatgtcat caatatgcaa
     6721 gactatggca tgatgaacgg agaaaaagtt cttgcacaag cagaaaatct aatgaaataa
     6781 atcaagtatg cagttggggg tataacaacg tggaagatca acaaaatcta gaagccatca
     6841 tggggttaat catgtttggc ggtaatgcaa agagcgatgc aatggaagcg attgcggctg
     6901 ctaaaatcgg tgattttgag ttagcggata ggaaaatcgc tgatgcagaa gaatcactta
     6961 tacaagcgca tcatgcacaa acagacatgt tgacacaaga agcacaagga aatcatatgc
     7021 aagtgacatt attgactgtt cacagtcaag atcacttaat gacatctatt gcgtttactg
     7081 atttagcaaa agaaatcatc gaactttatc gtcgcgtata cagcgaataa atacaacttc
     7141 ttttttgaac ggaagaagaa aaatttttga acggattttt tattttgtta taaggagaac
     7201 tctctttata taaaaaactt ataggaggaa gtaaaaaatg aacggactat caagttggat
     7261 ggagaaatac atcctgccgg ttgccgcaaa aatcggagcg caaaaacatt tagttgcttt
     7321 gcgtgatgct tttatcggca cactgcccgc aacaatggcg ggatcagtcg ctgtaatgat
     7381 caatgcaatt attcgtgatt taccgcaaca gtttaacagt acctatgctg acagtttagc
     7441 agcagacaaa ggaatctttg ccgtattgaa tgtcatcatc ggaatcaatg gttttgtctg
     7501 gaacggtaca ctagcaatcg ccggattgat ctttgccttt tcttgggggt ataatcttgc
     7561 ccgagcttat ggagtaaatg atctagctgg ggggatcgtt ggtttagcta ccttgattca
     7621 aggtatttcc tttggtccag cattatcagc agcattagat gtgaatgttc cttcaaatgt
     7681 cgcagatgca atcaatagtt ctgagattgg tgcaacgatt gcagatggca cactgtcagt
     7741 tggattatgg ggttggctaa aactagatca tttgaatgga aacgcttatt ttacagttat
     7801 gatcatggga gctatttcag tcattatttt ttgtaaattg atgcttgcca acattacgat
     7861 caaaatgcct gattcagtac caccagcagt atctaaagca tttgcagcca ttttaccagc
     7921 gacgatcgct ttatacgtca ttgctattat caattatatc gtaggtcgtg tagctgatgg
     7981 gcaactgatc attgatttgg tgcaaaaata tattgcagaa ccattcctag gattatctca
     8041 agggttggga gcagtattga tcgtcacagt atttgttcaa gtattctggt tctttggtat
     8101 tcatggacca aatgtattgg caccagtgct agagggaatc tggggacaag cacaactaat
     8161 caatattgat attttccaaa aaggttataa cggaaaaact ggtacaccag ccgtgttaga
     8221 tgcaatcgaa gatggaaaag cttatatgtg ggtccgcgga tcgttcgatg cttttgcatg
     8281 gtttggcgga tcaggcggaa cgatcgtctt actgatagct atcttgattt tctctaaacg
     8341 tgcagattat ctaacagtag ctaaattatc cattgggcct ggtatcttca atatcaacga
     8401 accagtgatg tttggtctgc caatcgtatt gaatgcaatc atgtttatcc cattcttgat
     8461 tgctccagta gtagcaacag caattggatg gacagctact tatcttggcc tagtcgctcc
     8521 ggtatcgcag caagtcacat gggtagtacc gccgttctta ttgtcattct tagcaacagg
     8581 agcggattgg cgggcaccga tcgtaacatt agtatgtatg gtcgtgactt tcttgatttg
     8641 ggcaccattt gtcatctctg caaacaaaat ggaccctgcc gcaggcgaac aacaataaga
     8701 tgaacgtgag gagcgggacg ctttaagttt cgttcctcat aatgaatttt cgaggaggaa
     8761 ctgaaatgat tacaattgcc agtgcaatgc aacaagatga aatcaaagaa ttgttagaag
     8821 gatacaatga agaaggaatg acttttacat ttaaagaaaa acaaggaatc aagctgttct
     8881 ttgaaacaag tgcagaagat ccggaagttg cggctaaaaa agcaaaggaa ctaatcaaag
     8941 cacagccttg gggaacagtc ctttattttc aatcaacagc tgtttagaaa atggtttaaa
     9001 aagataggat ggttgagatg ataaaaaacg gtttattttt atgtagtatt ggttctttga
     9061 tcgtgttgta tagtttgaat ccacattcgc aaaggtatga tttgtatagt atggtcacag
     9121 gtctttttct aacaggatta ggaatctatt tcttcatgaa ggggaaaaaa agagaagaag
     9181 caaaggagaa aaataactaa tggaaatcac gaaaaaatta aatattccag catcgttttt
     9241 ttacgataaa gttatggact ctgtcctttt cgatgtacgt aaagcgaccg gcaaatcagt
     9301 aactagaaaa cagctgaaaa atttggagta cgtcaaacaa ttctcgcaaa ataaccgggc
     9361 tcgcataaaa atcgaagaag tcattgaaaa ccagtcttat aagtttcgta catcgacaac
     9421 gaagaatgaa ttcgtcgttc attatcaaat caaaccattg gacgattcga cctgtgaggt
     9481 tcattacaca gaaaaaatgg agtcttatgg catgcttcaa aagttgaatg atatgctttt
     9541 aggaacaatc ttgatgtatt ttaaacggag acgtttcaaa aaaatgctag atatgatcga
     9601 agcaacgtat taaatcaatg aaacatggta ctatttagtt attgaaaaat gaagtaagct
     9661 agaaggaatt tttcatagga agatagatct ttcctgcgaa tcctcactag cttttttgta
     9721 ggaaggggca gcaaaatgac aaacaaagta agagagctag gtgtatcttc gattgaggaa
     9781 atgtttgatt tagccactta tgcatttaat ggggcagata cacctgaaag gcgagaacgt
     9841 tttcgtacac ttgcggagaa ttcttggaat tacggatttt ttgatgagga ggatcggcta
     9901 acaagtcaag tcatggcgac cccttttcca gtgaattttt acggacaaga gtacctaatg
     9961 gccggcattg gctatgttgc ttcttatcca gaagcaaggg gacaaggcgg gatcaatcag
    10021 atcatggaaa agatccttga agattgccgt gatcgtaaag taagcttatc ttatctggcg
    10081 ccattctcct atcccttcta tcggcgctat ggctatgaac aatcatttga taaaatcagt
    10141 tatcaactaa acagtcgtga tgtgccgttt atcaaaaaaa aatctggaaa aataaagcgt
    10201 atggattttg aagatgcaaa agctgcaatc agaagaattt atgaacagat gccggaaaac
    10261 caacgagctg gtctgaagcg cgaagactgg tggtatacgt ataaattcaa gcaaaaaggc
    10321 aattaccagt ttgctttgca ttttgatgat aatgaggaag caacgggata tatcgtttat
    10381 cagcttgcaa cgtcttcctt tattattgcg gagtggggat ttttggatca tgatgctttt
    10441 tgcagccttg ttcgcttcgt aagttctcac aatggtgctt ttgaaacatt tgaatattca
    10501 tgcggctacg gcggtaatga tcaaaattat ctattagaaa atccatttgc ttctgtaatg
    10561 atcacacctt atatgatggc gaggatcgta gatatttctt tgtttttgga aaattatcca
    10621 tttaaaaaga gacaagctgc atttgcccta caaatagaag aagatcatta tgcgaaatgg
    10681 aatgaaggaa tcgtggaagt aacaatcgaa aatggaaaaa atcatctctc aaaagtagaa
    10741 caaacaaaac tcccaatcgt tcgaggaagt atccaacata tcacgcagct attattaggg
    10801 tatcgtaaag tggaagagtt ggtattccaa gaaagaatac aggtgacaaa agaatatgaa
    10861 gaaacgttaa gctcaatctt gccacagcaa cagccaattc ttaatgacta tttttgataa
    10921 aaaacgggtg atgacaatat ttttgtcacc acccttttgt ttttcatttg gcatttgaag
    10981 gaaaatgtta aagtaaatcc tcttttttcg taaatttgaa ttttttgttt tcttttgttt
    11041 tagggttttc ttttttgtgg tcttggattg cttctttcgt aatcttttcc cgattttttt
    11101 tagatgaatc taagcgttct gcgtatactt tttcttttgg cttatcattc attggaaagg
    11161 acctccctac attatttcat tctacctcta gtttatcatc tgttaaggac tagtggaaac
    11221 gttttgccta acttataagc tgattttttt agcagataag cttgcaagta aaaaacaaac
    11281 aagctacaat tgatttattg aaattgttcc tgtagctcag taggatagag caatcgcctc
    11341 ctaagcgata ggtcgctggt tcaattccag tcaggaacgt ataaataacc cctgtatcct
    11401 ttgcggtaca ggggtttgtt ttgtatttgt gccaaaaatg tgccaaacta gttatcattt
    11461 ttatataaac tatcgaacac agtattgatt ttttctttat cctgatcttc aagctctcga
    11521 acaatatgaa gataagtaga cattgtagtt tctaaactgc tatgtcctaa acgtttcgat
    11581 acgctaagta tatttactcc ttgataaagt aaaatagatg catgtgtatg ccttagtcca
    11641 tgcaaagtaa attgcttttc aatccctaat tcttttaatt ttttgcgcaa atatttgctg
    11701 acggcgtttg atgaaactaa tccatttttt aagttgaaaa aaacaaaatt atgagggttt
    11761 ttgatttcaa aattctcgaa taattctttt tgattgattt taaacttttt taagagatca
    11821 atcgtatgtt tatcaatgga aattttccta tttgaagttt cgttttttgt ttttccccat
    11881 tcatttaatt tataattcca tgttttatta atagtgatga tttgatcttc gaaatcgata
    11941 tcttcccatg ttaatcctag aagttcagca aatcgcattc cagtggcacc agcaactaac
    12001 acgagcatag gagaagagta ctgcgcagaa aggttttctt ctacgagttt cattaaagat
    12061 ttaaattgat cataatctaa atatttatct tcttttggct tcaatgaatc ttttcctttg
    12121 attactgctt ttctagttgg atcaaacgga attaatcctt cttctacggc atctttcaac
    12181 gacgctctaa tatggttatt aaacttaata acggtagatt ttacatggtc tttagcatac
    12241 ttatttaaaa agcgttgata tcccactcta tctaaatcag aaatcaatac cgctggcata
    12301 tatttcttta tgttcattaa cgtatcttcg tattttctat aagtgatagg agatactgtt
    12361 ccttctttat aaagttgcat ccaatcctca aagtaatctg ataataatag atttttgcga
    12421 tcccctttaa ggcctttgtt caagttatat tctaattcgt tggcggcatc ttttgcttct
    12481 ccttttgtct taaatcctga ttttcttatc ttcgtgtatt ttccgtcatc ttttttgtat
    12541 gaaatttcgt attgccaact atttccacgt ttgactaatc ttgccataat tgaatcagct
    12601 ctctttctct gatacaatag gaactataaa gaagcccatt gtttgggttt tattattttt
    12661 tgcacatcct ttttgcttgc cggcgcaagg atgtgctatt tgtcgtttta atggtataat
    12721 tggatgtgta ttagatcatc caatcagagg tgtttattat gattaaagtg atttttaaaa
    12781 gaataactga acttccagag tttagattat ttgttgatta ttggtatgtc tggtttttta
    12841 ttgttgcatt gctgataggt ctagatgtca tattagaaag aaaacttaag taagagttga
    12901 gttaataaat tgaattccta aatgagatat tttgtagccc atattttgtt tgccgacatc
    12961 acctaatact atggagctaa tcgttttttc taacagacca aaatcagata atctatttaa
    13021 gctaccatct attagctctt gtgaatagcc agacatgctg atatttccag tgaaattttc
    13081 gtttccacga ctcattaagc atgctaacaa ttgaatctca atcgatgtta attgattcag
    13141 tacaagaaga taatactcct cgttatccca tgaagcgttg ttaattcgaa gtaaacaatt
    13201 cttataaaga tttttataca tatctatttt attttgtgcc tttgattttt ctacctcgtc
    13261 atttatccct tcgaatattc caatgagttg gtccttgttc tctaagttga aaacatcttc
    13321 tggaatttga tcgcgaatgt tttctaatga ttcgtttaaa tttttgtaga atttctcgat
    13381 gcgtttgaaa cgcttctcgt ttttagaccc aaaataaagt gactgcacaa cgccaccaat
    13441 ataaggaatt gctccaatac cgccttcaac tattaattca agatattctt ttttggttag
    13501 tttttcattg ctattatcca tagttacctc ctattttgtc atttccttta caagcattgt
    13561 gactcctgat ggcactcttt tgtataggtt ttattattgt gcacaatcta actcttagtg
    13621 gggcgtggtt catttatttt ttacttactt tactattaat catatcccat tgagcatagg
    13681 atttttctat gttcgatcct cttgaactaa tttctcctgt aaaagttact ttatcgccct
    13741 ttgtaaaggt atcagcattg tttaagtcct tcgcaaaaat tacatatgaa actttaggtg
    13801 ttgcagaaat atcattccaa cttttatcat cgtactcttt gtctgctata atggctattc
    13861 tagtagtcat tgattcgata acagttcctt caaaagtata tgttttacca ctaatatatt
    13921 tagtaaaagc tgtattttgc tcagtagctg gagtaataga ataataacta tctaagaaag
    13981 caccccagtc atgattattt gtctctaaaa attcttcaaa ttgagttagg cttttattgc
    14041 tgctgttaga tgtagtagtt tgagtatcag atgatgagct ttcctgtttg ttattgtcat
    14101 ttgcctcgtt tgatgaggaa agcgagaaac ctacggtagt aaaaataatc ccgataataa
    14161 ggagaatcat aggtgttttc ttatttgttt ttttgataaa tcttacaatt aacatgatta
    14221 aacctataat aaataaaaca ataccagtaa aaccaaatat accgtccatt aatagatcct
    14281 ctttctcttt tgatattgaa tattataaac caagcaattc ttttttctta aaatcgaatt
    14341 cttcttgatt aataatccct tcatctaaca actctttata ctttttaatt tcatctgcac
    14401 ttgaaatttg tgtttctttg gaattattat ctaatataga ttgttgcttc tctatatatt
    14461 tttttaaatt ttccatttca ttccaatatt tttttgtaaa cataacggtg ttttcatctt
    14521 tcgtggccgc taatatacct ccgcgacttt cgtttccgcc tagcaaggta aattgtatat
    14581 aaccattact catacctggt ttttttaatt gtacggctga tatattttta aaggggatag
    14641 ttttttctcc tttaatacct tgattaacta aatttataaa tccttttcta ataattgaaa
    14701 tagaatcatc agttacttta atcaaggttt ttcctggaga ttttattaaa atctcttttt
    14761 ccattttttc ttcctcattt ctgtgatatg attttttttg tagaagctca tatatgagga
    14821 aagagcccgt gttgcagcac gggctttttt aatgttttgc tgaaatcggt ttttttaata
    14881 att
//
LOCUS       DACCXO010000063        14832 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.064, whole genome shotgun sequence.
ACCESSION   DACCXO010000063 DACCXO010000000
VERSION     DACCXO010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.064"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            201..1097
                     /locus_tag="IVY70_001782"
     CDS             201..1097
                     /locus_tag="IVY70_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP7958909.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1247..2188)
                     /locus_tag="IVY70_001783"
     CDS             complement(1247..2188)
                     /locus_tag="IVY70_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP7958910.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2314..3435
                     /locus_tag="IVY70_001784"
     CDS             2314..3435
                     /locus_tag="IVY70_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7958911.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3693..5195
                     /locus_tag="IVY70_001785"
     CDS             3693..5195
                     /locus_tag="IVY70_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP7958912.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5262..5789
                     /locus_tag="IVY70_001786"
     CDS             5262..5789
                     /locus_tag="IVY70_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7958913.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            6000..7061
                     /locus_tag="IVY70_001787"
     CDS             6000..7061
                     /locus_tag="IVY70_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP7958914.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7064..7960
                     /gene="murQ"
                     /locus_tag="IVY70_001788"
     CDS             7064..7960
                     /gene="murQ"
                     /locus_tag="IVY70_001788"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP7958915.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7974..9956
                     /locus_tag="IVY70_001789"
     CDS             7974..9956
                     /locus_tag="IVY70_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7958916.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(10021..10467)
                     /gene="nrdI"
                     /locus_tag="IVY70_001790"
     CDS             complement(10021..10467)
                     /gene="nrdI"
                     /locus_tag="IVY70_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP7958917.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10678..12102
                     /locus_tag="IVY70_001791"
     CDS             10678..12102
                     /locus_tag="IVY70_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7958918.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12283..13056)
                     /locus_tag="IVY70_001792"
     CDS             complement(12283..13056)
                     /locus_tag="IVY70_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7958919.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(13053..13958)
                     /locus_tag="IVY70_001793"
     CDS             complement(13053..13958)
                     /locus_tag="IVY70_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7958920.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(14112..14432)
                     /locus_tag="IVY70_001794"
     CDS             complement(14112..14432)
                     /locus_tag="IVY70_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP7958921.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         4762 a         2630 c         3120 g         4320 t
ORIGIN      
        1 gactaatgat aacaaatttt cagacaatta caactattca gaaaactttt aggcgaattt
       61 taatagaaga atgatttatt tcatcatatt ttcatacaaa tgttaaattt tcatacaaat
      121 ataggaaacg ttttactggc tttttactat ttaaacaggc tatcatagga aaaagtgaca
      181 gagaaagtga ggcggaaaag atgaacaaaa atgacgtact gctagttgca aaaaataaat
      241 cactttattt tttgccttcc atccgctctt tagatgtttg gtccggaaaa gatttactac
      301 agtcggaatt cattaaatca gctagacaac agttggcaaa ttatcagctg atcgtttttt
      361 tagattatgg atttgatatg gagatggctc gacgcgtgcg cccatttacc aatgcaaaaa
      421 ttgtgttatt tttttggaat cacttcaaag cagaacacga ggcgatgctg aagcaggcac
      481 aagatgaacc ggcaatcgat gagatttatc attttgattt tttggaagca aaagaacttg
      541 ggctgaaaca taatagttca ttttattcga aacatatgaa tttttcttct agcgaaccaa
      601 agacggatct attttttggt gcaacgaata atgggagaaa agaacgagca gaaagctata
      661 agcaggaatt tataaaaaga gatgttacga ccaactattt cattcttcca catcgaggga
      721 atgaacaacc tggatatctt acttatgaag agtacctgaa aaaaacaggg gaatcgcgcg
      781 ggattctgga actgttgcgg gagggtcagc aaggagtgac tttgcggaca tttgaatcca
      841 tgtattttca aaagaaacta gtgacggata atcaagcgat tgctcattat catttttatc
      901 gtcctgaaaa tatttttttg cttcgagaac gtgatttaga tgaattaccg gaatttctgc
      961 atacaccatt taagccaatc ctatcagagc tattggaatt ttatgatgca gaaaattggg
     1021 caaaacgatt tatcactaac gattccacta tttatgagca atacgaatat gacctctcct
     1081 ctgttgaaaa caaataaaaa acctacattt tgaagagatt atgtactgaa acaaacaagc
     1141 acaattctta tggcatcatg agagttgtgt tttctttttg atcttttatt gggcttaaaa
     1201 aaagagaacc agctgctgat tcagctgatt ctcatcagag agccttttat tctgcgccaa
     1261 ggattccttc ttttgcatgg actaattggg tcaaaaaggc acaatatggt gtagcaatat
     1321 catatttttg tccttttcgc caaatggcac cattgatgta gtcgatttca gtcaaacgat
     1381 ggtttttgat caggtcttgg tacatagatg ggtaatgcaa cccgatgttg tcaggatcat
     1441 aagtagaagc aatatgttgg tacacttctt cttgatctag tatgattcct tcttttgctg
     1501 cgatagaagc aaattcagat acgattgttc gaaccatgct ctctgccgct ttttgcgcgc
     1561 ctagttcagc aatattacaa tccaaaatcg tgcataaacc attcaatgtt ccatttacac
     1621 aagcttttcg ccaaatggaa taacgaacgt tatggctata gatcggattt aaatttgctt
     1681 cttgaaagac ttctacaact ttttttgtaa atgcttcgcc ttctggttcc aagttctcta
     1741 gttcgatttc tccatcacct aacaatgtga cttttccggg accagctaga ccagctgtcc
     1801 acatcgtgat cccaaataag atatttttct ttggcacata tttttccagt acatcttcat
     1861 gacctaatcc atttagtaaa cacaaaacat aagtattgtc agtaataatg gattggatcg
     1921 aacaaaacat atcatctaat tgattggctt ttgttaatgc tacgatcaag tccacgtgtt
     1981 cgttgctttc gatgatttct tctggtgaat agattggcag ttttgcgacc acttctttac
     2041 cgttaaagtc agcaatcaac ccatttgtac gaatggcttc aatgtgagca ggccaacgat
     2101 cgatcaagag gacctcattt ccgctttgat gtagcatcaa tccgattcgg ctgcccattg
     2161 ctccggcccc agctattgcg attttcatgt tttttctctc ctttattcaa tgagttccct
     2221 tttagtatat agaaaaatgg aagaaaatag cattgttttg tttgaaaaaa atgaaaaaaa
     2281 gtgtcttact taagaagaaa gtgagggata cagatggata ttttggcggc aattgattca
     2341 tttaaaggaa gtgcttcttc atttgaacta aatcaggcag ttctaaatgg ctgggaagat
     2401 cgtaaaggaa gaaaaatcaa tgtgccgatt gcagatggcg gagaaaatac tgcagaagca
     2461 atctattacg cgctcagggg aacatggcgt accattgatg ggatggatct cttattccgt
     2521 cagcgaaaaa tcagctattt attgacaact tggcaaggaa aaaacaccgc agtcatcgaa
     2581 tctgcacaag cattggggtt agatctactt gataaaccaa cggatcaaac tatccgactt
     2641 gcttccagtt atgccttagg agaacttatt aaagatgcac tgcttcaaga agtagaacaa
     2701 attattgtca ctttaggagg aagcgggtgt agcgatggtg gtctaggatt actccaatcc
     2761 ttgggtgctt ctttagaagg gtacacagaa ggaaatcctt tattatctac taaaaaactt
     2821 cattttgatc gaagaaacgt tcttgtttct aaagtaaaat tgattatcgc tgcagatgtt
     2881 accaatccat atacaggtca acaaggagct gcttggcaat ttggaagaca aaaaggagga
     2941 atagatacga cgctagcttt cttagataaa caagcaggaa agattgctca gcaaataaaa
     3001 gatttctggg aggtggattt agatgatatt ccaggaagtg gagcagcagg cggtattggt
     3061 ggagcccttt ttttgttggg agcgaagatg gtttctggtt tcgatttagt cagccaaatc
     3121 actggattgg aaaaaaagat cgaacaagca gatgtcatca ttacaggaga agggcggatc
     3181 gatcagcaaa cgatccacgg aaaagttcca tacggtgtag cagtgcttgc aaaaaaatat
     3241 cgaaaaccaa taatcgctat ttgcggaagt agagaccaag agctagaagg actttctgat
     3301 tttctcccaa ttgtcttaag tattcatttg ggtccaatag gtttagaaga agcaatggaa
     3361 cacaggcaaa cattagaaaa aacaaaaatc ttaggacaga cacttagcag atttttcacc
     3421 atcctcccga agtagttgac aaaatcattt tctttatgct atcgtaattt caaatatagg
     3481 acaaagtgag ggatccgtgc attgaaaaat taagtcgatt gatgaaagct gcattcagac
     3541 tgtaccttgt ggagacaagg aagcagtgtg cagattttcg ggattggctt ggttttttcg
     3601 atgtatgggt ctttttgtgt gtatccaacc cttgcatcct ctttagccag ttctgaaaat
     3661 aggactggtc tttttttacg aggtgatgga aaatgtctaa aatcgaaata aaaaatctga
     3721 cattcggcta cgacagccaa ggcacattat tatttgaaca agcaaatcta aattttgaca
     3781 cacaatggaa actaggactt atcggacgaa acggtcgagg aaagacaact ttactgaata
     3841 ttctacaaaa caaactacct tatcaagggc aagtaatcca tcagcaagaa tttgcctatt
     3901 tcccgcaaca gacaaaagat aaagaacgtt taacctatta cgtgttaaat gatattacgg
     3961 attttgagat atgggaaatc gaaagagagc tccaattgat gcaaacagat cctgaaatct
     4021 tatggagaga attcagcaca ctatcggggg gagagaagac aaaagtccta ctggcacttt
     4081 tatttgtgga tgacactcat ttcccgttaa tcgatgaacc aacgaatcat ttggatatct
     4141 ctggtagaaa acaagtagcg gcttatttga aaaagaaaaa acaaggcttc atcgtggtca
     4201 gccatgaccg gggatttatc gatgaagtag tggaccatgt tttagcaatc gaaaaaagtc
     4261 aactggaact ttatcaaggg aatttctcta tctatgaaga acagaaaaaa cttcgtgatg
     4321 aatttgaaat ggctcaaaat gaaaaattga aaaaagaagt cagtaggcta aagaaaacag
     4381 cagctgaaaa agccgaatgg tctcgttccc gagaaggaga taaaacaaag aaacaagtcg
     4441 gattcatcga tactgaatct agacgagtga ataaaggagc agtgggtgct gatgctgcac
     4501 ggacgatgaa acgatccaaa gcaatcgtga atcggatgga gacccagatc agcgagaaag
     4561 aaaaactatt aaaagatatc gaatatatcg attctttgac gatgaatagc caagcgtctc
     4621 accataagcg acttttaagc gtagaagatc ttcaattagg gtatgaaaat ctgttattcg
     4681 agccaattca ttttacaatc gagcctcatc agcgggtggc gatttcaggt cctaacggtg
     4741 caggaaagtc atccattatc cattatcttc tgggggcatt caacggcaag gttataggag
     4801 aaaaatacca gccaaaacat ctgagcatta gttatgcaag ccaaaattat gaagacaatc
     4861 gaggaacgtt ggcggaattt gcagagaaaa accaagtaga ctaccaagca tttttgaaca
     4921 acctccgaaa gcttgggatg gaaagagatg tttttcataa caagatcgag cagatgagta
     4981 tgggccaacg gaaaaaagtg gaattggcta aatctttatc acagccagct gaactatata
     5041 catgggatga accattgaat tatttggatg tcttcaatca agaacaatta gaacaactga
     5101 tcttgaacgt gaaacctgcc atgttactag tggaacatga tcaaaccttc ctggataaag
     5161 tatctactga gattatttct cttgagagaa tctaaccctt gtaatctcaa gaacaacaag
     5221 gtatggttaa ggggaacttt gccaacgaag gagagaatca catgctttat gtaacgagac
     5281 acggagaaac aacttggaac gcacaaggat tagtttgcgg acgggcagat gtgccgctga
     5341 ctgaaaaagg acagatgcag gcacagaaac tagcagaaaa ggtagtagat ttgcctgttc
     5401 caatcacgaa aatcatccat tcccctcttc aacgagcaag agatacagca caagcagttg
     5461 ctgatcgtct cagcttgccg ctgaccgttg atgaacgatt ggtagaaatg gattttgggg
     5521 attatgatgg actgccaagc aaagatgaga actttcaaaa agcacgttta gcgttcgctg
     5581 tacgattccc aaatggggag tcagtcttag atgtgtatgc acggatcgtt cctttattga
     5641 aagaatgcat cgaagacgaa gaaaatgtct atctgttagt ttgccataat gccttgataa
     5701 gagtgatcaa tgcttatttt catccgatgc ctaatgaggg gttctttaca ttcatggtag
     5761 ataatacaga attggtttct tatgagtaat tatgtttttg tgttatttaa ataaaatatt
     5821 tcaaggcact gcttttgctt agcagtgcct tttttaggtt atgacaattc ttttgataat
     5881 ataaactcaa cctttagaaa tgaaatttaa ttattttaat aaaagtattg aaattttgtt
     5941 tctttagata tatactctag tcatagaaat gaagacggat acagaaaaag agggataata
     6001 tgtttggctt ttcaatattt atgaacgatt ctttgtcaga agagaagaaa cagtatatcc
     6061 gccaaatggc atccaatggc tttgtcggta ttttcacttc catgcatata ccagaagatg
     6121 atgtttcggc atatcaaaag cgattgatcg aactaggaag cgaagcgaaa aagaataaac
     6181 tagaattgat ggtcgatatt tcaggagagg cattggaacg agcgggtttt tcattagaag
     6241 atctaacgcc tttgaaagca attggtgtaa caggtttaag aatggattat catatttcca
     6301 atcagcagat tgccgactgg tcacatcaat tgaaaatcag tttgaatgct agcacgatca
     6361 cgcctaaaga tatcgatgaa ttgaaagaag cagaagctga tttctcacag attgaagctt
     6421 ggcataatta ttatccacgt cccgaaacag ggctggacaa agaatggtat cagaaaaaaa
     6481 atcaatggct caaaattcaa ggtctccttg tccaaggttt tgtaccagga gatacagagt
     6541 tgagaggccc actatatcaa ggattgccaa ctttggaaga acatcgaggg atccatcctc
     6601 ttgcaggcgc tctagatcta ttagcaagta acacggatat tgtttacatc ggagatgcag
     6661 gtctgtcaga aaacgttcag gagcaatttg cttctttcca aaaagaacag acagtcttgc
     6721 tccatacgga acctgtagat gaagaatttt acgaatatat attagggaag catacgaatc
     6781 ggcaagacga tgcaagagat gtcatacgaa gcgtagatgc acgttttcga gagattccgc
     6841 ctatcccagc aagaaataca gctacgcgta tgaaagggtc tattacttta gataacgaaa
     6901 agtatttgcg ctatatgggt gagatccaat taaccaaata tgatttgcca gcggatgaaa
     6961 aagtgaatgt agtagctaaa gttatcaaag aagagctacc attgatcaac caaatcaaag
     7021 ctggtatgaa ctatcaattt ataagaaaag aaggaagata aaaatgagca aaatcaattt
     7081 agagaaccta acaacggaac gcagaaacca agaaactttt ggattagatg agatgagtat
     7141 ttctcaagcc gttcaactaa tgaacaaaga agatcagaaa gtggccgctg cggttgaaaa
     7201 agaactgaca agtatcgaac cagtgattgc taacacgatt gagtccttta aaaaaggtgg
     7261 acgtctgatt tatatgggag ccggaacaag tggaagacta ggtgtgttag atgctgctga
     7321 atgcgtaccg acattcggtg tagaaccgga aatggtcata ggtctaattg ctggtggaga
     7381 agaagcaatg actgtagctg tcgaaggggc cgaagattct tatgagctag ggcaagaaga
     7441 tctaatcaag ctgaatttga caaaaaatga tatagttatt ggtatcgcag ctagcggtcg
     7501 tacaccatat gtgatcggtg gattagatta cgcaaaatcg atcggtgctg tcacaggaac
     7561 gatttcttgc aataaacaag ccgaaatcag taaacacgcg gatttcccaa tcgaagtcga
     7621 tgcaggtcca gaatttttga caggctctac tcgattgaag tcaggaacag cacaaaaact
     7681 tatcttgaat atgatttcta ctatcagtat gattggtata ggtaaagtat tcaataactt
     7741 gatggtcgat gtcaaaccaa caaatgaaaa actaatcgaa cgctcaaaac gaatcatcat
     7801 gcaagcgaca gaagctgatt acgaaacagc tgaaaagtac tttgaagaag cagatcaaaa
     7861 tgttaagctg gctatcgtca tgattttgac aaatagtgat aaagaggaag ctcaagcaaa
     7921 attagctgaa gcaaacggat ttgtaaaagg gaccttatga gggggaaaag accatggcaa
     7981 aaaacgaact gactttggaa gaactggcaa aacaaattta taaaggggca ggcggcatgg
     8041 gcaatgtaga aagcattgtc cactgcatga ctcgtgtgcg tatgaagatc atcaatgatg
     8101 atcttgtaaa tgtctctgaa ttaaaagcga ttccaggagt gcttggtgta gtagaagatg
     8161 aacagcttca agtcatcatc ggacctggga aagtgaacaa agtagcacag caaatggttg
     8221 atttggcagg cgtgaaatta ggagaaaaac tttcttctgt aagtacagct acagcaaatt
     8281 catcagcatc tggaaaagac cgtgtaaacg aacgggcaca agaaatgaaa gcggcacaaa
     8341 aatcgaaaca aaaaccatct aaaatcaaat cggtattaaa agacatttct aatatctttg
     8401 tgccaatgat tcctgcattt gtcggtactg ggatcgtggc agggattgcc gcagttttag
     8461 caaacttagt cacagccgga acacttgatg cagcaacatg gcaacaatat atcgatgtga
     8521 tgaacatctt gaaaaacggg atgtttagct acctagttat ctatacaggt atcaatgctg
     8581 cacaagtttt tggtgcaaca ccaactcttg gtggagttat cggggccgtt gtgatgctga
     8641 cagggatgaa tccggaagca ccactgaaaa acttatttac tggtgaagct cttgcagctg
     8701 gacaaggtgg gatcctaggt gtcatcttcg ctgtttggct attgtcaatc gttgagaaaa
     8761 aactgcataa agtagtacct gatgcggtag atatcatcgt tacaccaact ctttcattga
     8821 ttgccatcgg tctgattgaa attttcttca tcatgccttt agcaggtttc atttctgatg
     8881 gcatggtcgg tgggatcaac tgggtacttg gcgttggtgg agcatttgcc ggcttcgttc
     8941 taggcacatt attcttgccg atggttatgt tcggactgca tcaaattttg actccaatcc
     9001 atgtgcaaat gattgatgaa actggtcgta cgctattgct tccaattctt gcgatggctg
     9061 gtgcaggaca agtgggtgca gcattggcat tatggattcg ctgcaaaaaa gataaagaat
     9121 taacagaaat gatcaaagga gctttaccag tcgggatcct aggaatcggc gaaccattga
     9181 tttacggagt aacattacct ctaggacgcc catttattac tgcttgtatc ggtggaggta
     9241 tcggcggggc agtgatcgga atgatcggaa atgtcggggc tatcgctatt ggaccttcag
     9301 gagccgcttt gatcccattg atctctgacg gtaaatggtt aggctacatc cttggattat
     9361 tggcagctta tgctggtggc ttcgttgcta cattcttctt cggtattcct aaagaccgtt
     9421 tagaaaaaga agaattagcg actgaaacag ccacagtaaa tgaagtgcct gaaaaagtag
     9481 tacctcaaac ttcagctgaa agtgtgacta tcctctcatc agttgctgac ggaacagttg
     9541 tttctttaga taaagcaagt gatccagttt ttgcacaaaa gatgatgggt gaaggctact
     9601 ttgtagaacc agaaaacgga catatctatt ctccagttac tggggtagtc agctctgtct
     9661 tcccaacgaa acacgctatt gggatcacaa cgccaaatgg tttagaaatc ttgcttcata
     9721 tgggaatcaa cacagttgac ttaggcggta cgccatttga tgtgaaagta acagaagggc
     9781 agcaagtgac acctgatacg cttgtagcag aagctgactt agaagcgatc aaagcagcag
     9841 gcaaagaaac atccatgatc gtcttggtaa ccaatatgga tcgtgtaaaa aactttgtct
     9901 tggaaaaaac aggtaaagca aaagcaaaag cacctgtgat ggatgtcaaa gcttaatatg
     9961 atagaaacaa tagaaacgaa agaagaaact gaaatctatt tttcagcttc ttctttttta
    10021 ttatttattg ttttctgcta aaatctgata aattcgtgtc acatcagatg gtgttccccg
    10081 taattcataa tctgccaaga aaggaaagcc aaatgtttgt gcatattgtt tggctgttag
    10141 acaatactga ttattgaagt ttttatttcc gctaccgact acaccaagac aaagtttatg
    10201 attgtcttca tgttccagat attcacgcat cgtttcggtc aagatttctg tatcaccgtt
    10261 atctaaacca tttccgccgt ctaaataagt aggaacgaaa gtaaaaaatg gttctgtttc
    10321 tttcgtaaaa tcactatttt catggatttc ttttaaagta acttcaggat tgacaggatc
    10381 ttctgcatgc attttttcag catattctgc tagatgtttg gcaaaggcac gcgtatttcc
    10441 tgaaatcgat atatataaaa tgttcatgga tcatcctcca attctttcct tttagtatag
    10501 catctttttt aagttttttg gtggtgtaat ttggaaaggg actaggcgct ttgcttgtat
    10561 aatgagaaaa aagccggtag aattatattt ctattaaagt ttttattaat taagtaaaaa
    10621 taaaaaaggt ttgttatact ttttgataaa agcggtttta ttttggagga tgcttacatg
    10681 gatacagagc aacagaaaaa aggaattggt ttaattccac ttgctgcctt agtaattggt
    10741 tcagcgattg gaggtggcgt atttggtatt atgacggaca tctccggatc ggctggcggc
    10801 ccagctattt tcagttgggt actcgtagga acagcaatgt tgatgctctc cttgtcgatc
    10861 aataatatca ataaaaaacg tccagattta aaaggcggga tctttagtta cgcagaagaa
    10921 ggttttggcc gtttcgctgg ttttatcagc ggttggggtt actggctcac atgctggtta
    10981 ggcaacgtag cttttgcaac attgttgatg agcgcattag gatatttctt cccagtattt
    11041 gaaggtgggc aaaatctggc atcgatcgtg atgagtacag tctttttatg gggatatgca
    11101 tggctagtga atcggggagt cgaaaatgct agtttcgtca atgctatcgt cacattctgt
    11161 aaattagtgc cattattttt atttattgta gtagctatca gcacgttttc gatccaacaa
    11221 ttcattgata acttttctag cgacattata ttgaatgcga atggacagcc gatcccgttt
    11281 tccagtcaag tattgagttg catcatggtg atgctttggg tatttgttgg tattgaagga
    11341 gcctctgtcg taggttcacg agcaaaaaaa caatcagatg ttgggaaagc aacgattcta
    11401 gggatattcg gcttgattat tatttacgtc ttggtttcca tgcttccata tgggacaatg
    11461 acgatgaatc agctgcaaac gatcaaggcg cagcctgcta tgggctatgt gtttgaaatg
    11521 atggttggta aatggggagc agtcgtgatc aacggcggtt tgatcatttc tttagttggt
    11581 gtttggctct catggacgat cttaccgatt gaaacaatgg gaaacatggc tgaagacgga
    11641 cttttgccta aagcatgggg aaaactcaac aaaaatgggg caccaactta tgctatcgta
    11701 ttgacgacgt tatgtacgaa tgtcttttta ctttcattat tattcactga cagtgcttat
    11761 aactttgctt acacacttgg tacagcagct attttcttca cgtggctttt cttaggattg
    11821 taccaaatga aactttctta tcagcggaaa gaatgggtcc aatttttagt agggctttta
    11881 gcaagtgcct ttcaagtatg ggctatgcta gtcgtagcct ttaatgaagt aatgcttgtt
    11941 cttgttcttt tcttgccagg catttatttt tacctcaaag cacgtaagga gcaaaacgag
    12001 cctgtacaac atatccactt agataagatt ttacttatag gtgtgacgat ggtcggagtg
    12061 attgcaatgg ttttatttgc cactgggatg cttcacgtat agaagtataa aagaagtata
    12121 aagagaggtt gtgagcctgt cgtttattca aaaacatact aggaaaggag ctgtgacaca
    12181 aatgttgtca cagctccttt ttcagaataa acggtgttta agagacagcc aatcggcaat
    12241 aaggctgaac gtctctttca cagcctcttt tttgatgagt catcatacat tttttcgctt
    12301 aaatacgaga tatcctaatg ctaagaacaa aacgatgtaa gctaaattac cgaatagcat
    12361 ttgattagta gaaagatgag tcaattgctt catgatctct tcgtttccaa tttggttttg
    12421 cagattcaac atactgatcg gattccattt gatccactcc catttttgga tgagtgcaat
    12481 aaggatacca gatatgattg acgaagcaaa ataaaagacg attcctgctg agatggatgc
    12541 gccggaacta ttgataaagc aagcaatcat caacactaaa cttaaaatca gccataaacc
    12601 gatataacta cccaaagtat aagtgaacaa gctttggatc agtgtttgac cagtagaaac
    12661 cttctccgtg aaggaaatcg aagggaaaag aatcagtttc atcaacacag tgacaataat
    12721 agctaaaagg tataaataaa cagaataaat aactaatgtg atccatttgc ttactaatat
    12781 ttctcctcga gaatactttc gataaaggag atttttcaat gtaccatatt gtgcttccat
    12841 cgaaatgatc gtgcttgcag ctgcaatcat gataaagaca atccaagatg tggcactaaa
    12901 aagctgagca gtcatttcaa ctggatcaat gatcgtcgtg tattttttgg ctagaagtgc
    12961 tgttcctatg agcaaaacaa ctaatagaac agataaaaca gcagttgact ttttcttgat
    13021 caatttaaat aattcttgtt ttacaagcaa tgtcattttt cttcttcccc ctttactgaa
    13081 tccagaattt ccaatagaga agattccaaa tcattcgtca gatgctggac gtctgtcaca
    13141 tggatatttt gtgcagacaa tagctggatc acggttccta ataattcttc gttttctttt
    13201 aaaagcttga ttttatcagg atcttccaaa gtatatccag cttctgtcaa gatcgttcga
    13261 gcagctagat catttgttgt agagataacg atgaaatctt gcgctgtttg gttcaattca
    13321 gccattgtac attgtttgat gatctttcct tgattgatca ggatcagctc atcgaccatt
    13381 ttttcaagct cgctgagtaa gtgactggag atcaagaaag aggtgccctg agtagccaag
    13441 tcggtaatga tttttcgcac atctcttgtt gcttgtggat ccaaaccgtt catcggctca
    13501 tctaaaataa cgatagacgg atggttcagt aaggcaagag caattcccaa tttttgtttc
    13561 attcccaacg agtaggattt cgcttttttg tgaatgtact tttccatctt gagctgattc
    13621 acgatagtgt cgatagcaga tacatcgttg gtttcagaga ataacttcag atgatcatag
    13681 cctgttaaga atggatagat acccgggtat tcgatcaatg ctcctacttg ttccaaccct
    13741 ttatgactag agggagaaat ctcattttca ccaatacaga ttgttccatc tgagtaatgg
    13801 atcagtccta aaatagcttt catgatcgtt gtttttccag cgccattagg acctactaat
    13861 ccaacgattt tgccagggga taagctaaaa ctgacatcgc tgagggcttg tttgtgacca
    13921 aagttcttgg ataattcttg gaccgaaaga cttttcaaaa atattcactc cttttctata
    13981 gatagtacaa gcatacacct gatgagtgga gaagtcgcta ggtcattgga cttatttctt
    14041 attttttcat aaaaaaattg aatattaaat aaaaaggctt attttttctg atcagtactt
    14101 attccaagtg tctagtggcc gctagtaatc ttgatgccaa tgatactgat gagtagcatc
    14161 cccacaaaga accaagtgga aagattcagc ttatcatgaa acaagaccac accaattatg
    14221 accgaaccaa ctgcgccaat ccctgtccag ataggataag agagactcat cggcaaactt
    14281 ttgattgcct ttgacaaaaa gtaaaaacta gcaatcatgc caatcactgt gtaaaaagaa
    14341 taattgattt tagaaaaacc atcactccat ttcatcatcg ttgaccagaa tacttccaaa
    14401 attcctgcta tgattaattc catccatgcc atacttacat tcctccttaa ctataaaaaa
    14461 atacacaaaa agaggaaaat catcccttca aagacctaat ggaatgatca tcctctttat
    14521 cctacccggt ggcagattgt atatcttttt ctgtagtata gcacacctat ccagatatcg
    14581 gcaatctcat actttttagt aaagtgtacc aagcactgac ggatatatcg ttagcggaaa
    14641 agaagatgaa tgctttgcaa tttcatagaa agaagttgtt atttattaat catttaaaaa
    14701 aaacagtaac tagaccttat ttctatatat tatcgggcaa taaattaaaa aaagctttac
    14761 cttacaataa gtaagataat ttttgtgata aaaaaataac gaaaaatatt taaaaatagt
    14821 gtctatttcg tg
//
LOCUS       DACCXO010000064        14820 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.065, whole genome shotgun sequence.
ACCESSION   DACCXO010000064 DACCXO010000000
VERSION     DACCXO010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.065"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(296..1024)
                     /locus_tag="IVY70_001795"
     CDS             complement(296..1024)
                     /locus_tag="IVY70_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7958922.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="IVY70_001796"
     CDS             complement(1002..2576)
                     /locus_tag="IVY70_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7958923.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="IVY70_001797"
     CDS             2805..3743
                     /locus_tag="IVY70_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7958924.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="IVY70_001798"
     CDS             3755..4666
                     /locus_tag="IVY70_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7958925.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="IVY70_001799"
     CDS             4690..6162
                     /locus_tag="IVY70_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7958926.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="IVY70_001800"
     CDS             6213..6824
                     /locus_tag="IVY70_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP7958927.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="IVY70_001801"
     CDS             6854..8767
                     /locus_tag="IVY70_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAP7958928.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="IVY70_001802"
     CDS             8895..11876
                     /locus_tag="IVY70_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HAP7958929.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="IVY70_001803"
     CDS             11873..13534
                     /locus_tag="IVY70_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HAP7958930.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="IVY70_001804"
     CDS             13574..14506
                     /locus_tag="IVY70_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP7958931.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4818 a         2332 c         2979 g         4691 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagtgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagttgaaa
//
LOCUS       DACCXO010000065        14699 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.066, whole genome shotgun sequence.
ACCESSION   DACCXO010000065 DACCXO010000000
VERSION     DACCXO010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.066"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(20..733)
                     /locus_tag="IVY70_001805"
     CDS             complement(20..733)
                     /locus_tag="IVY70_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7958932.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            908..2332
                     /locus_tag="IVY70_001806"
     CDS             908..2332
                     /locus_tag="IVY70_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7958933.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2631..3338
                     /locus_tag="IVY70_001807"
     CDS             2631..3338
                     /locus_tag="IVY70_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP7958934.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIALYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            3325..3648
                     /locus_tag="IVY70_001808"
     CDS             3325..3648
                     /locus_tag="IVY70_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP7958935.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(3737..4774)
                     /locus_tag="IVY70_001809"
     CDS             complement(3737..4774)
                     /locus_tag="IVY70_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7958936.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPTDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5095..6078)
                     /locus_tag="IVY70_001810"
     CDS             complement(5095..6078)
                     /locus_tag="IVY70_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP7958937.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(6493..7263)
                     /locus_tag="IVY70_001811"
     CDS             complement(6493..7263)
                     /locus_tag="IVY70_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7958938.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7256..8191)
                     /locus_tag="IVY70_001812"
     CDS             complement(7256..8191)
                     /locus_tag="IVY70_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7958939.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8507..10156)
                     /locus_tag="IVY70_001813"
     CDS             complement(8507..10156)
                     /locus_tag="IVY70_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP7958940.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(10273..12876)
                     /locus_tag="IVY70_001814"
     CDS             complement(10273..12876)
                     /locus_tag="IVY70_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP7958941.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12888..13937)
                     /locus_tag="IVY70_001815"
     CDS             complement(12888..13937)
                     /locus_tag="IVY70_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP7958942.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13963..14277)
                     /locus_tag="IVY70_001816"
     CDS             complement(13963..14277)
                     /locus_tag="IVY70_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP7958943.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(14293..>14699)
                     /locus_tag="IVY70_001817"
     CDS             complement(14293..>14699)
                     /locus_tag="IVY70_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7958944.1"
                     /translation="LNASDKKSVVEKLSKLLETNGFVSDHKQFQKDVFDRENHMTTGI
                     GKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLLAIKDTDKGTNHLRI
                     LAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
BASE COUNT         4351 a         3050 c         2485 g         4813 t
ORIGIN      
        1 aaaaataatt ttcccgagct tatttttcaa caaaaacagc ttcaaaaaca aagtcttgat
       61 ataaatgtct cgataccgaa tactcgaaag gtttaccaga cgatagagca acaactttgt
      121 ctatctcaat cataaattca cctggatcta tttttaatat ttttgcgtca aattcattcg
      181 cagtatctcc tttgatacgt atgtgcgaag tctttatttc cagacccagt tcatttgtga
      241 tataagcata tacagatttc tttaaatgtt ttggttccaa gcctggtatg actgctaatg
      301 gcatgaatgt ttgctcaatc gagtatggtt gacaatcaat aaaacggctt cgtacaatat
      361 tatatacata gtcttttaca ctgatactta atttatcagc tacttttttc gatggaagtt
      421 caattgagaa agtatggatt tctgaatcta cgtgttcttc accaaccacg ctaaacaaac
      481 cttcgtttgg accaagaact cgtgagttat caattggtgt tctgacgact tcagtcccat
      541 acccacttct acttcgcaaa actccttcgg aaacaagtaa gtctacgcct ttctttactg
      601 ttaacttgct tgtatgataa ataactgcca attcatctcc tgtaggaagt ttctcaccaa
      661 ttttatactc tccatttaca atttttttct ttatatctaa gtaaatttcc atatacttcg
      721 caattttttt caatggtgac cctccaatca aaaacatatc ttcatctata ctttaacata
      781 aataaaccga taaaatccac atcaaaatta tacacacaat aaaaaagata tatatattta
      841 aacaaaacta tttactttaa gttgtaagcg gtgttatcat taagtggaaa caggaggtaa
      901 aaaaattatg acaactatga aaaagctaac ttttccagat gatttttggt ggggttccgc
      961 ttggtcagct gagcaagcag aaggaaaagg agaaacagga aaagcagaga ctatctggga
     1021 tcgctggttt aaagaacaac cttaccggtt ttatcaacga atcgattcct ctattacaac
     1081 cgatcacatc catcaatata aagaagatat agaactaatg aaaaaaactg ggcataactc
     1141 ctttcgcgtt tctatttctt gggcacgaat gtttccaaat ggcggtaccg gaaaagtaaa
     1201 tcccaaagcc atttctttct atcgaaaatt attttccgaa atgaataata acggaatcaa
     1261 agtatttgcc aacttatatc attttgacat gcctgctact cttcaagata aaggaggatg
     1321 ggaatcacgt gaagtagttg atgcttatgt tcattacgct gaaacttgtt ttaaagaatt
     1381 tggtgactta gtttgtcatt ggtttacctt caatgaacca cttgggccta ttttaggtac
     1441 ttatcttgaa gattttcatt atccaaacct cattgacttt aaacgcggag cgcaagcagc
     1501 attcttcact atacttgcac acgcaaaagc aatacaagta tttaaagaac tagctcttcc
     1561 tagtaaaata ggcgttattc taaatttgag tcctactttt cctcgtagtc aaaatcctgc
     1621 tgatgtgaaa gcagcagaaa tcgctgattt attttatacg cggagctttt tagatcctat
     1681 ggttaaaggg aatttttcta aaaagcttat cctgttatta caagaatata atcagcttcc
     1741 tgaagattat acgaaagaag atcttgtatg tatctacgaa aataccgctc aaattcttgg
     1801 actaaactat tacgagccac gtcgcgtcaa atctcgttct tctatggtgg atacatctag
     1861 tccattttta ccagagtggt ttttcgaacc acatatcatg cctggacgac gaatgaacga
     1921 ataccgggga tgggaaattt acgaaaaagg aatttatgat ttgtgcatgg atatcaagaa
     1981 ccattatgga aacatcgaag catttatctc ggagaacgga atgggcgttg cggatgaaga
     2041 acgtttcatg gatgatacag ggcaaataat tgatgattat cggattgagt atatcaaaga
     2101 tcatcttgct tacttatgga aggcaataaa tgaaggatgt aacattaagg ggtatcatct
     2161 ttggacattt attgattgct ggtcatggat caacgcctat aaaaaccgat atgggctggt
     2221 ttcactcgac cttactactc aaaaaagaac aattaaaaaa agtggcgaat tttacaagca
     2281 attaagtgat acaaatggat ttagatacga tgtagacttg ttagttgaat aatattctaa
     2341 aattccactt gaagtgaaca cattagaaaa caagaataat gagaaatacc aataacaaat
     2401 actttttctt catacttcat ttatacttgt cagtataaga gactgtgaca aatgtagtga
     2461 tcctcagaag ttaggtctgg aaatctaact tgagaagatt tttttaaact acgtccagtc
     2521 ttttttattt gtttaaaatt gaagttgaca agtcttcatt aaaaatttag aatattcaca
     2581 agtttgacga caattatgaa aatttatgtc gtgctaaagg agttttgtta ttggatgaaa
     2641 agttagatat aaaaactgcg atcaaagaca cgttacctac cgtttttggt tatatcggta
     2701 ttggacttgc atttggtatc gttgggaaag ctgccgggtt tcatccgctg gtcgtcacgt
     2761 tgatgtccct gctgatctat gctggttctg cccaatttat cacggtcagc atgcttgcta
     2821 gccatagtcc actgctctcc atcgttctct cgacctttct agtcaattcc cgaatgattc
     2881 tgatgagtat gacgattgct ctttatttca agaaaaatcg gctgcttcaa aatctgctga
     2941 ttggcacatt gctgacagat gaaagtttcg cactgggaat gaataaattg aactatactg
     3001 gacaaaagtt aaactttcgt tggatgaata cagctaattg gatttcttat ttgacgtggg
     3061 tgggttcttc tcttgttggc gcacttttag ggaattttat tactgatccg aaaaagtttg
     3121 gtttggattt cgccatcgtc gctatgttta tcgggttact ttatcttcaa gtcatttctg
     3181 atagaaacac ctctaaacga ttacagttga tcctgattgg tctcactttg attctggtat
     3241 atgtcggact gatttttatc ccaagtaact tagtgatttt agttgtaaca ctgattgggt
     3301 gcggattggg ggtgtggatc aaacatgcct tcttttgagt ttacattgct gactatttta
     3361 ggttgtactt tagcaacttg gctttctagg atcctaccat ttgtcttact gaaaaaattc
     3421 gatttgccgc aaccgttgct tgagtattta agttttgtcc cgatcgtcat tatgtctgct
     3481 ttatggttca gcagtctatt tacccaaaat atcggacatc tgccgcagat caatctcgaa
     3541 aatgctttgg cttcagttcc tacactttta gctgctattc tttcaaaaag cttattggtg
     3601 atcgtcttgg taggaattct ttccctttcg ttgattcgct tattataaat aaaaaaacaa
     3661 gagcttatgg caaattactg tcatgcgctc ttgtttttat caaagtcaaa taagaaaaaa
     3721 atcggcgtat tcttgcttat ccgttgaacg gaccactaac aacgactttt cctaatatat
     3781 gtcctgtttc tacgtctttg gttgcttgtt tcaagttctt cgcattcatc ccatctgaat
     3841 agaccttgct caatgtactg cggataatcc cttcatttgc taaagcagca atgtgcgcta
     3901 aagcttctcc ttgcgtttcg atttcatagt tatagtctgt tttagcgaac atatactccc
     3961 agtcgaagct gatagattgg tttttccaaa cacctatatc taacggctct ttgatcccta
     4021 cgatcgttcc cacatgtcca aatggtcgga caaggtgttt gatttgatca aagtagcggg
     4081 taatatcgaa taatacagcg acatactcta cttgatcgat tcctaattca cgaaaacttt
     4141 tacttaagtc gtgatgataa tcggttggat agtctacacc tgtttttttc agccattcaa
     4201 aattcttcgg acttgcagtc gcgtagactt ctaggccagc ccaatgcgct agttgattca
     4261 aaatcgaacc gacgccccca gctccgttga tcactaagat ttttttcccg cggttggcat
     4321 tttcttctgg agtcagacca aatttttcaa ataacaattc ataagcggtc aatgaggtca
     4381 acggcaaggc tgcggcttct tcatctgaaa gattttttgg tgcaagagcg acaatacgtt
     4441 cgtcaaccag ttggtattct tgattgcttc ctgctcttgt cgttgtacct gcgtaaaaaa
     4501 cgcgatcgcc gacagaaaat ttttgcactt catcacctac agcaacaact ttcccgactc
     4561 catcaaaccc taagatacgt aaagcatttc ttatcccgtt atcttggcga agtttcgtat
     4621 cgacaggatt aacagatact gcgttgactt ttaccagaag atcccgtgaa ctgggtacag
     4681 gtatctgttc tttttcatca ataaagcttt ttggatcatc gatcgacaac ccttcataaa
     4741 aaccgactac cttcattaat tccttttctg acatacgaat tcctccttag cttcatcaag
     4801 ttggagcatc catacagttt tcatttcaat atatatagta tgctatttgt tccactttta
     4861 ttcatcataa ttattctctt acttaaagta aactatttgc acttttttgg ttaaaaaaga
     4921 ggttgcgaca agacttttgt cacaacctct tttaaacggt gttcaatcaa ctgaccctcg
     4981 gtattcgctc aaaaaacgac aaaatatgag gagctatttt tgtttggctg ttcttattac
     5041 ttgatgcagg acagcttttt cacgacctct aattcacggt gttcaagcaa gaaatcattg
     5101 ttcactgaaa agcgttggat gttctttttc aaaggtgtct aagatctctc gtacttcatc
     5161 agtagatttc gtattcatca actgaactcg caagtcattt gctcctggaa atcctttgat
     5221 atagatttta aagaaacgat gtagtcctgt gatggaacgc gggatgattt ctgaataatg
     5281 atcttgcaga tctagttgca gacgaagtag tccgatcagt tctttttctg tatgttccct
     5341 cggttctttt tcaaatgcat aaggattctt gaaaatcccc cggccaatca tgatgccatc
     5401 tacgccatat ttttcggcca attcttctcc tgtctggcga tctgggatat ctccgttgat
     5461 cgtgatcagt gtttgcggag cgatttggtc acgtaaagca atgattttag ggatgacttc
     5521 ccaatgtgca tctactttac tcatctcttt acgcgtacgc aaatggacag aaagattggc
     5581 tatatcttga tgaagtaggt gactgatcca ttcttccatc tcagacattt ccgtatagcc
     5641 gatacgcgtc ttgacgctga caggcaaacc gccagcttta gccgcttcaa tcaattgtgc
     5701 cgctacttct gggcgaagga tcaagccact gccttttcca cgttctgcta cattagggac
     5761 tgggcagccc atgttgatat cgattccttg gaagcccatt tctgccatag caatgctcat
     5821 ttctctaaaa aattctggct tgtctcccca aatatgggca accatcggct gttcatcttc
     5881 tgtaaaggtc aatcttcctc gtacactgtc ttttccatct ggatgacaga aactgtctga
     5941 gtttgtaaac tcagtaaaga agacatccgg tgctgctgca tgctttacga catggcgaaa
     6001 tacaacatct gttacatctt ccattggtgc taaaacaaaa aaaggctttg gcagctcttt
     6061 ccaaaaatta gtagtcattt atttgttact ctccattcta tcaagcagtg aagtcgcaat
     6121 ctttctcaaa aacattgtca ttatactagg aaacaaaaaa agaagcaaag tgaacagctt
     6181 aaaagatgtt tctgattgag caaaaaaaga ctgtgacaaa aatcttatca caattccttt
     6241 tccggaataa atggtgttca aaagcttgca tctgcggtaa taagtccaac caaacgaaaa
     6301 ataggaggag ctatttttgt ttggctgttc ttattacttg atgcagaaca gctttttcac
     6361 aacctctcca ctaaacggtg ttcaatcagc tgaccctcgg tattagctca acaaacgaca
     6421 aaatatgagg aactattttc ccgtttgttt ctctaatact caggtcaaaa cgctgatttc
     6481 acaacctctt ttctaacttc tcaatcctct tcctttattg atcaaatacc aagtaacggc
     6541 gtacaaaaca acgatgaata gcacaagtac gatcatcgat gttgctactg gcacatcgat
     6601 tgttccaagg aatccgtaac ggaaaccgga gatcatataa acgatcggat tgactttcga
     6661 aacagcttgc caaaatggcg gcaacataga gatggcatag aaaacgccac ctagataagt
     6721 taacggctgc aagacaaatg taggcacgat cgatacatcg tcataagact gagcaaaaat
     6781 cccgttgagc agtcctgcta acgaaaacaa gattgccgtc atcaataatg taatgatgac
     6841 catgaaccat gaatacacat gaagtggtac aaagaacaag gaaatgatcg ttacgagaga
     6901 gccgacgaga atactgcgac ccactccgcc aatcacaaag ccccaaatga tcacatgcgt
     6961 aggaacaggc gctaccaaga tttcttcgat gtttttctgg aatttttgag agaagaagga
     7021 cgaagaaacg ttggcatacg aactagtgat agcagacatc ataatcaaac caggcacgat
     7081 gaattccata tatgaaaaac cacccatgtc accgatccga ccaccgatca tttttccgaa
     7141 aataacaaag tacaaggatg tcgtgatcac aggcggaacc agtgtttgga cccagatacg
     7201 caaatagcgg ttcgtttcct tgatagctaa actttttaat gcagtaaaat aaagattaaa
     7261 catttttctc ctccgtatga ttattttctt ctgtgatttt caaaaacagt tcttctaaac
     7321 gattcgactt attcctcata gaaagaactt tgattccttg tcttgtcagc tgatcgaaga
     7381 ttccgttcac cccttgattt cgttcaactt cgacagcgat tgtctgttca tcttcaaatg
     7441 tacaagcgta tccttcaatc acaggtttgg tctcatatgg cgctaaatca aagataaatg
     7501 tttcaaattg cagtttggat agaagctctt tcatactagt attctcgatc agttcacctg
     7561 attggatgat cccgatgttg cgacaaagca tttcagcttc ttctaaataa tgcgtcgtca
     7621 aaatgatcgt tgtaccattt tcgttcaatt ctcggagaaa ctcccacatc tcgcgtctca
     7681 attcaatatc gactcctgct gttggttcat ccaagatcag caaacgtggt tcatgcatca
     7741 atgccctagc aatcatcagt cttcgcttca ttcccccgga aagcatacgt gcacggacat
     7801 ttctcttttc ccaaagattg gactgcttca aatatttttc gctgcgtttc atcgcttctt
     7861 tgcgagagac accgtagtag cctgcttgat tcacgacgat ttgctggacc gtttcaaagg
     7921 gattgaaatt gaactcttgc gggactaatc caatctgttg ctttgctcgg actagatccg
     7981 tatccagatc ataaccgaag acattcactt ttccagatgt tttattgact aaagaagtaa
     8041 tgattccgat cgttgtagac ttcccagctc catttggtcc taatagcgca tagaaatctc
     8101 cttctttcac ggaaagatcg attcctcgca atgcttcgac cccacttcca taaacctttt
     8161 tcaaattatt tatttcaagc gcatttgcca tttatttcct atctccttat agacatgatt
     8221 ctttattgta tcatggaata cagcagatga aaaaacaact tggttccagc gaaaaatcag
     8281 gttgctgggc tcaggaaaat ctcctaatga aagacatcaa tcggacgaag tcagacaacc
     8341 ttttcagaaa aaacaaatcg aagataaagg attactattc atatgtgcaa caaaaggagc
     8401 gtgagacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaag
     8461 caaaagccaa gatttttgat ccaataaata ctttcgatca cttcgcctat cccaatacat
     8521 gccactgccg cttttcatca aataacgcgg cttccgtttt tgcggtttct actaaagtct
     8581 ctgggtaatc caaaactttc agcaaggcaa tcgcatttcg ggtccttgaa ggtccttctt
     8641 ttaatttgaa atcaaaactg attccttttc cttctgttac ttgttcttca aagtggacat
     8701 tctcacaata attctttaaa atctctgtca attcgatatc atgtgtggcg acaaatgcta
     8761 aagatggata ctccgcaagc caccgaacga ctgaagcgga agaagcaatc cgttcgatcg
     8821 tatttgtccc ttttaaaatc tcatctacga agcagtagca gcgttctttg gaagccactt
     8881 ggtccaacaa acgtttgatc gatttgattt ctgaaacaaa ataactgtcg ccttcaaata
     8941 aatcatcttc gattgccatg gaagtcaaca catgtccagg ttgcagggta aactgttctg
     9001 caatcgctgt ttggatcgtc tgtgctagaa tacagctgat ggctatactt ttgacatacg
     9061 tagattttcc tgatgcattg gaacccgtga ctaatgtatt cttctgccag ttgaccggat
     9121 tcactaccgc ttcttttaac aatggatgat agatatcttt cgctgtcaca ccgccctcag
     9181 taaactctgg tcggcaagtg atgggcatat aagtacggta attcaatatt gcagcggcta
     9241 cttctaattc tcccagcaat tcccacagct tgatggcttc agttgaatgc ttttctaatt
     9301 tagtgagaac gaaatgatag gagataaatg gcagcatgaa catgatgttg acatattcaa
     9361 aaatcaactc tgcttcactc ccactcttca tacgaaatga aaaacctaat ttagggatgt
     9421 ctcttaatgg tttagtttgc tgtttcagct cttcttgaag tggtgtcggc agttttgcta
     9481 gctgattggc ggctgaaatc gttgaaacca aataactcat gctattcagc tctgtttcta
     9541 gtgtttgttt cttcactaga taatagaccg tattgaatac taacccaccg attgccaaat
     9601 agattcctag tacttgtcct aaaagaagca aacccaaacc caccagcgga agcaagccaa
     9661 gaaaaataaa taaacctacg cgtccaatct ttttactttt tccatccgct agatattgtt
     9721 ttgcaaaatt atgatctttt tttcctaaac gagcgaattg atattggatt ttttctctca
     9781 gttgaggatt ttcctgataa aaagcaatca gtttttctaa ctgctgatct tcaccaaaat
     9841 caaagctgcg taaacgttga tacaacgctt ctgaacctac actagaatag gtactgttga
     9901 ttccttcgaa tacttcgaac atatccaggt cataccaagt aagatcatcg atttcgctat
     9961 cccatgaacg gaacttcttc tccgtgagcc atgcttcctt caagctttct tctttatcaa
    10021 atctcggttg ataaggaatt ttttcccatt tgctacgaac tgtttccttt aatttcaaac
    10081 gtgaaaaaat gtctaaagca actaataaaa taacagctgc aataataatt aaaacgataa
    10141 attctctttg acccatatcc ctctccgttc ctttatttgt tagctttagt atagcactgt
    10201 tgcgctattt gaaaagaggg taaaaaaaca cctttcaaaa taaaaggtgt ttttttcttg
    10261 tatattacat atttatctct catttatcaa aattgacaaa taatcatagg gaggaattgt
    10321 tgttaattct tgtttattct ccagtgcatt gacaactgtc ccaagggaaa ggatagcctc
    10381 aggtactatt ttttcttgtt ctgttggatt agctaagcga agaacaaatc cttgttcatg
    10441 aaaataagaa ggataaatcg atgatacaat taagtcttca ggtaattcaa tcaatgaaaa
    10501 tttacgagga acatcaagtt cattttgtaa tggccaaatc ttgttatcta acctgtgaat
    10561 aaatacattc agtttctgct tttgataaga aatagattgt tctaaccgtt tttctgcctg
    10621 ttttgccact ttgaactcat taaatcttcc ttcttctaca ttaaatccaa aagtgacttt
    10681 atactcacct atctcttgag ctagcggtgt ttccatcatg acatgccctt catttgtcgt
    10741 atctcctgaa gctcgcccag gtcgccaagc aagattaggt tttccaagtt cccctgttgt
    10801 tgaaaataag gttatgatta attgattttc tatacgttca tactctttta aaccatctgc
    10861 ataaaacgta aaataatgat cattttctgc cacagagaca ctcttatcga atatttctaa
    10921 attaacaggt ttctctacca tagtttctga ccaattcgga ggaatgacat caggggtatt
    10981 ttcaataaat ccgttttgaa tttgtgcaat tgatttttca gatttatcta aaacattaat
    11041 tgctaagcgc atacgatgcg agtaaacagt attatgaatt ttgagtgtgc cttcaacgat
    11101 ttgactttct cccagactga acgatatttc ataagatatt tcttccattt cgggtttttc
    11161 agataaacga tcttttaaat ctttaggaag ttgagctttt ccgtaaacaa ctaatgtttc
    11221 ttgtaaagaa tctttatata ctttagctgt ttctaatttt agagatagtt ctgtatctcc
    11281 ttctaatggt gaataatcat acgtatcgcc atcatttgct gaatcgatca aatgaacgaa
    11341 atcaagatat tgtctatctc caactatcaa attcaattgc ccatctttaa aagaaagttt
    11401 atatttattg ttacttatct cagttttgtc tatctcgatt gctgtttcta atttttcatt
    11461 atttttctca aattcaacaa cagtgtatcc tagtgctggc acatttacat ttatttttat
    11521 gtcaagttca taataagctg gttcatcaaa atattcgaaa cctgtagcaa tcatccgata
    11581 agcatttttc cgttctggaa aataaacttc tttttcaatg atattatagg gactaccttt
    11641 gaagtgtatt ttctttgaat cagctactac atgcacgatt ttcgatccac tataattctc
    11701 agtcgttgtg ttgaaaacaa ggacttcatt cgattttagc tttaatgcat cagccatccg
    11761 ttttacaata aggttttcga ttccgtcagc tatctcatta gcttctttga ttcgatggaa
    11821 aatatcttcc gcaacattat ctgaaacaca gccaccaata ctatcatgag cctggttttg
    11881 caaaatcttt ttccataacc gacgaagtag gcctttactg atatggatac catttctttc
    11941 tgcaatcagc atgagcggct caactcgttt tagaattttt tgttccaatt taaaattttc
    12001 tattttcatt tttgttctta ctgagccaat agaacgatga acacgagagt aaaccggctg
    12061 acgaagttca cctttatatt caggtaattc ttttttgttt ctgataactt ctacataaga
    12121 aggataatct gaaataatat tgtggaattt actgtattta ttcaacttct ctacgacttg
    12181 atcaaaattt aaaatcattg acttttgatc tacacccgaa ggtactaaaa tatctttgtt
    12241 ttttccttgc atatgaagca tttcaactgc ttcatccaac ttttcttttg caaattcagg
    12301 aatatcttta attgcgtcta aggtcatcat tccagtcata taaccaaatg gaaaattcat
    12361 ggcataaaca aatttatctc ccaatccttt ccagataaaa taaggagatg gaaccatcgt
    12421 ttcaaagttt aatcctcgcc aacacatgaa attatcgatt tctacttgat gcaaaagtgt
    12481 aggcaaattc gcattgaacc caaacgtgtc aggcaaatac cctaccatca ttggatctcc
    12541 atatttaagg atcgtatcac tgattccaat cattaagttt cttaaaattg attcagcatc
    12601 cactaataag gcatctgttt gtgcatacca cggtcccacg aatagttgtc ctgttttcat
    12661 cagtcgttgg ataaccttaa gcttttgcgg attgatctcc aaatactcat cgacgattga
    12721 agtttgtcca tctaaacaaa agtttacttt agggttcctt tccaattcat ctagaacttc
    12781 agtaaagacc tgatcagaca atacgatggc atcttcattt gtaaaatacc attctctgtc
    12841 ccagtgcgtg tgttgaatta tatgtgcatt taccatatca gattctccta ctttccttct
    12901 tctactgaat ttacttgttg ttctttttta tacttgatgg attttagtgc attgaccatt
    12961 aacgctgtaa ccactccccc aataacaatt gatactaaat ataaaggtat attcgtaaca
    13021 aaaggaattg ccataaaggt tgaaagcatt gtttcttgag taacaccaaa aaatccattt
    13081 agaccacatg ctactgcagt gcccaccacg atgcttggta ttactcgtaa aggatctttc
    13141 acggcaaacg ggatcgtccc ttcagtaatc cccacaaggc ccataatcat agctgtcttt
    13201 gcattatctc gttccgattc atcataaagt tttttgttta aaattgttgc taaaaacatt
    13261 ccaaatggcg gaatagcaat tcctaataac atggctgtac taggtccaaa gcttccgctt
    13321 gaaaaagaag caaccccaaa agcatatgcg attttattga taggtcctcc catatcaaac
    13381 gccatcattc ctcctaaaat agccgacatc aaagcagcat ttgctcctga catgccttct
    13441 agccaattag tcagtcctgt tgtcattgct tgaataggcg tagctagtac ataccataat
    13501 atgattccaa caactaatgt agtaacaaaa ggaatcagca taattgattt tagactaata
    13561 acttgcatcg gtacttttac ttttttcaat gcattagcta aatagccaca agctaatcca
    13621 gcaaccattc ctccaataaa tcccatacca agattttgac acatcatccc agtgataaaa
    13681 gctggtgcta gaccgggacg gtcagcaatt gaatatgcga tataggctga aatcatcggt
    13741 acgataaact gtaatcccgt ctgaccaatc attcgcagga ctccccagaa tgtatccgtc
    13801 tggtcccaaa cacctgtgcc accaaaaatg actgcaatag aagttagtaa ccctgctgct
    13861 acgacaagcg ggatcatata ggaaacccca ctttggaaat gatttagaat ttctcttcct
    13921 tgtttcttga attccattag taactccccc acgatatatt ttttatttgt taattaatgc
    13981 aattgcttca tcaatcgtct cttcaccatc ttttactacc tctgaggtcc ctactgttaa
    14041 aatcggttta ccgtcaaagc gacctcgttc tgaaacattt gtatctactg caaatatgac
    14101 aacatctgcc tcggaaacat ccgaaattga aagtttgttt tctatcccca tagccccctg
    14161 tgtttctact ttaatatcta accctttctt cttcgcaaac ttttctaaat tagcttttgc
    14221 catataagta tgggcaatcc ctgcgataca tgctgttact gctactattt ttgtcatatg
    14281 aaattcctcc ttttaaaatg atagagcttt taccaattct tttttatttc tcgaatcttt
    14341 tattctttta acaaactcgt catccatcaa tcttccagat aattctgcta aaatacgtaa
    14401 atgattagta cctttatcag tgtcttttat tgctaacaaa aagacgagat caactggttg
    14461 atcatctaat gcgccccatt caacagaccg ctttaacttg gctacagcca ctgttgattc
    14521 aattactgct aggcttttgc catgaggaat ggcgatattt ttcccaattc ctgtggtcat
    14581 atggttttct ctgtcaaata cgtctttttg aaattgtttg tggtcagaca caaacccatt
    14641 agtctctaac aatttcgata atttttcgac tacacttttt ttatcagatg catttaaat
//
LOCUS       DACCXO010000066        14474 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.067, whole genome shotgun sequence.
ACCESSION   DACCXO010000066 DACCXO010000000
VERSION     DACCXO010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.067"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            67..2361
                     /locus_tag="IVY70_001818"
     CDS             67..2361
                     /locus_tag="IVY70_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP7958945.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            2354..3019
                     /gene="pgmB"
                     /locus_tag="IVY70_001819"
     CDS             2354..3019
                     /gene="pgmB"
                     /locus_tag="IVY70_001819"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP7958946.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            3012..4019
                     /locus_tag="IVY70_001820"
     CDS             3012..4019
                     /locus_tag="IVY70_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP7958947.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            4041..5060
                     /locus_tag="IVY70_001821"
     CDS             4041..5060
                     /locus_tag="IVY70_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7958948.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(5341..6708)
                     /gene="nhaC"
                     /locus_tag="IVY70_001822"
     CDS             complement(5341..6708)
                     /gene="nhaC"
                     /locus_tag="IVY70_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP7958949.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(6817..8238)
                     /locus_tag="IVY70_001823"
     CDS             complement(6817..8238)
                     /locus_tag="IVY70_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7958950.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(8481..10358)
                     /gene="tdc"
                     /locus_tag="IVY70_001824"
     CDS             complement(8481..10358)
                     /gene="tdc"
                     /locus_tag="IVY70_001824"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP7958951.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(10649..11905)
                     /locus_tag="IVY70_001825"
     CDS             complement(10649..11905)
                     /locus_tag="IVY70_001825"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP7958952.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            12485..13786
                     /locus_tag="IVY70_001826"
     CDS             12485..13786
                     /locus_tag="IVY70_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP7958953.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(13855..14454)
                     /locus_tag="IVY70_001827"
     CDS             complement(13855..14454)
                     /locus_tag="IVY70_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7958954.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         4492 a         2998 c         2701 g         4283 t
ORIGIN      
        1 ctattgactt gtgcaatcgg ttgcgtttat tatgaacgta taaaacagct cattaggagg
       61 agaaaaatga aacagatcaa acgactattt gctattgatc catggaaaat cagcacgaca
      121 caattagata aaaaaaatct tcgactgcaa gaatctttaa caagtattgg aaacggttat
      181 atggggatgc gcgggaactt tgaagaaact tactcagggg accaccatca aggtacttat
      241 ttggcgggtg tctggtatcc cgataagaca cgtgtcggct ggtggaaaaa tggttatcca
      301 gaatactttg gaaaagtgat caacgccttg aactttatcg cgatagatat ttcgatcaac
      361 gatcagctga tcgacttggc ttctttggaa ccagaagact tttactggga attagatatg
      421 aaaaatggtg tattatctcg tacattcaca atcactactt ctactaataa agtacgcatt
      481 tccttcgaac gttttctgag cattgtcaaa aaagaatcag cttatatcta tttaactata
      541 gaaatgcttg aaggtagcgg aacagtcaaa ctcgtatcaa aattggatgg aaatgttcaa
      601 aacgaagaca gcaactatga tgaacatttt tggaatgaga ttgatcgtgg aactatagaa
      661 gaaatcggct atttgacgac taaaactatt ccaaacaact ttgggattga acaatttact
      721 gttactgcag caatgcgcca tttcgttaat ggaaaatcag tcatccctga ctatcacgaa
      781 gatactttag cattacatgg ttgcttgtct gcagaattat ctcaaggtga gacatggtca
      841 gtggaaaaag aaattatcgt tcttactagt agagatattc cagaagcgca gcaagcagaa
      901 aaagctgtct cagaattaac gaaccagaca aacaattata tccaagcaaa agaagagcaa
      961 acagcggctt gggcagaacg atggaagctt gccgatgtgg tcatcgaagg agacgaagaa
     1021 gcacaacaag gtattcgatt caatttgttc caactattct ctacttacta cggcgaagat
     1081 gaacgattga atatcggccc caaaggattc acaggagaaa aatacggcgg agccacttac
     1141 tgggatacag aagcgtatgc tgtccctctt tatcttgctt tagccaaacc tgaagtaact
     1201 aagaatctgc tgaaatatcg gcataatcaa ctgccgcaag caatccacaa cgctcagcaa
     1261 caaggattaa aaggcgcgct ttatcctatg gtcaccttca ctggtgtcga atgccataac
     1321 gagtgggaaa tcacattcga ggagattcac cgaaatggcg cgattgccta tgcaatctac
     1381 aattatacga attacaccgg cgatacggaa tatttgaaaa atgaaggact ggaagtttta
     1441 gcagaaatcg cacgcttctg ggcagaccgt gtccattttt caaaacgagc aaataaatac
     1501 atgatccacg gtgtgaccgg cccaaacgaa tatgagaaca atatcaataa caattggtat
     1561 acaaatacga tcgcagcttg ggtcttgcgt tacacgcgag aaaattattt aacttatcaa
     1621 aaagaagctg ctatcactat cagcgaagaa gaactagcca aatggaaaga tatcgcagag
     1681 aacatgtact tcccagttga cgaagaatta ggaatctttg tccagcatga cacattctta
     1741 gataaagatc tcatgcctgt atccgatatt cccacaagcg atcttcctct gaatcagcat
     1801 tggtcttggg acaaaatcct tagatcttgc ttcatcaagc aggcagatgt actacagggg
     1861 atttatttct tcaatgatac ttttacaaaa gaagaaaagc aaaaaaactt tgatttctat
     1921 gaaccaatga ctgtccatga gtcttccctt tcgccaagta tccattcgat tttagcagca
     1981 gaattaggaa tggaaagcaa agctgtagaa atgtaccagc gaacagctcg cttggatttg
     2041 gataattaca ataacgacac agaagacggc ttgcacatca cttccatgac tggtagctgg
     2101 ttgtcgattg tccaaggatt tgctcaaatg aaaactgccc atgatcagtt gagattcgca
     2161 ccatttctgc caaaaacttg gaaagcttat tctttccata tcaattatcg cggacgttta
     2221 ttgttcatct cagtcacaga tgaacaagtt actttcgagt tattagaagg ttctcctcta
     2281 gaaatttacg tctatgaaca gtcttattct ttagcggatc gtctagaaat aacgctaaga
     2341 aaggaggaag caaatgttta aaggggtatt gtttgattta gacggtgtca ttaccgatac
     2401 tgctgaattc cattatcgtg cttggcgaaa gcttggtcag gaaatcggca ttacgatcga
     2461 ccacacattc aacgaacagc taaaaggcgt cagccgagaa gactcactct ctttgctttt
     2521 agcttatggc ggaaaagaac actctttttc aaaggaagaa tttgcagaat tagcgaaaag
     2581 aaaaaacgat tattatttag agatgatcca gacgatcgaa ccaaaagatg tcttccccgg
     2641 ggtcgtgcct ttactggatt ctttaaaaga agaaaaaatc aagattgctc ttgcttctgc
     2701 aagtaaaaac gggccattcc tacttgaaaa aatgggtctt acaccttact ttgatgctat
     2761 tgccgacccg gcagaagcaa caaacggaaa acctgcgcca gatatcttcc tccttgcagc
     2821 aaaagctgtc ggactaaaag cagaagaatg tatcgggatc gaagacgcgc aagccggtat
     2881 ccaagccatc ctctcaagtg gcgctcaacc aattggcgtg ggacgtgctg aagaccttgg
     2941 agaggagatc ccgcttgtcc ctgatacgac ttttcttacg atcgattact tgaaagaaaa
     3001 gtggcatgac catggataat ccagagatct ccgtcgtacc agttccagag actgaaataa
     3061 agaaaatcac attgaagaac gactacttag aagtcgttct tctcaattac ggtgcccgcc
     3121 tgcaccaaat cttcgcacca gacaaagaag gaaaatcaga aaatgtttta ttgtcttacg
     3181 atcgtttcga agatgttcta acagacaaaa gcttttttgg agcaactgtc ggacctgtag
     3241 ccggaaggat ccgggatgct tcttggggtg agcatagatt ggagaaaaac tgcggatcac
     3301 accatatcca cggtggcacg aaaggctggt ctttccaatt ttgggaagta gaagttttca
     3361 aaactccatc atcaataggt gtggtctttt acttaaaaga tgaattttct acctatccag
     3421 gaccaatcac tgctacgatc acttaccgtt taacaaaaaa cgaattagag atgacaacct
     3481 ctgctagtag ccttgttgaa accatctgca atccgaccaa ccatgcctac ttcaaccttt
     3541 caggaaatgg aacaagagac atctatgaac atcagttaac ggtcttttta gatggtgtgc
     3601 ttgaattaga tcaagaaaaa ctgcctactg gaaattggac aaaaaaagaa aaactgccaa
     3661 tcgactttag aaaatcccca acactgcaag agatccttgc atgttatcca gatggactag
     3721 atgatgtatt tcttttgcat catcctaggc tttccaaaac ttccttacaa ttattcgaga
     3781 agcattctgg tcgccaaatg actatagcaa cttctaataa aagtatggta ttattttcta
     3841 cgactggatt cgaagctgat ttctctgtca atggaaaaca gatgcattca aactacggat
     3901 tagcaatcga accgcaagaa atccctgata tcgttcattt ccccaaattt ggatcgatca
     3961 acctccatcc tggtcaagaa cggatcagtc agacgattta tcggttctct gcacaataac
     4021 aagagaaagg tgtttttatc atggctatta ccgtaaaaga tgtggcgaaa aaagccggtg
     4081 ttgccacctc gaccgtctct cgtgtcatca acgatcatcc tagtatttcc gagacaacaa
     4141 agaagaaagt ccgaaaaatt atggatgaac taggttatgt tcctaacatg actgcccgca
     4201 atctaggaaa acgcatttcc agtgccatcg gcgtaatctt accccctctt gattcaaaag
     4261 agcggatcgg taacccattt tatcttgaga tcatggaagc aatcaacgaa gaagcgcgga
     4321 attatgatat gactacagca atcgctactg ctaaaaattt tgaattgctg ttagagaacg
     4381 tccagcgtat gcatttacaa aaacaagtag atggttttat tctagtgtat tcagatagtc
     4441 aagatactgt cattgattat ttataccaaa accacatccc tttcactttg atcggacagc
     4501 cttatcatca tgagaacgaa atcgtttatg tagataatga taaccagctt ctaggtaaac
     4561 aagcaacaga atttttgatc cagaatggac ataagaatat cctgtttgtg acaaacacga
     4621 ctcatgaaag tttgtatttc gaacgatatt tcggttatca aaaagcaatg atgatggctg
     4681 gtcttcctgc gttatccagt gtagtactag agcatccgga ggattatctg tcctttgaga
     4741 aaacgttgaa agaaaacgaa gccacagcat tggtggtcat tgacgatatc ttcgcccttc
     4801 gcacgatgca gttggcgcaa atgtatggct atgtcattcc agagacactc tccgtcatta
     4861 gcttcaacaa ctcgatcttc tcaacattaa cacaccctta ccttacaagt atcgatatcg
     4921 atatctccga attaggacgt gtcgctatgc aaaagctgcg cgaattgatc catgaagaat
     4981 tgagcagtgg cgtacgtttg gtcatccctc atcgcttgat caaaagagag acagtcgttc
     5041 ctttagaaaa cccatactaa atagctgact cactaaatac gctgaccatt tatcctatgg
     5101 tcagcttttt atgtaaaaga agatactact tctaacacat tgtttatctg taaaaaagag
     5161 cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta aacacgtata aacggcggaa
     5221 gcagaagcaa ctccttcgga aataagctga aattcacaaa aatttaaaaa gcaattttct
     5281 tgagtttctt cttatttctt ggagttaaac gcttctgtcc cagcctcttt tttccttcct
     5341 tcaatttttt acacgtcgac gattcaataa aaaaccgcct aatattgtaa agacaggtgc
     5401 aagaatcgag aaaaaggcga atggcaagta atgcaacgcg ctaactttca tcgttcccat
     5461 aatgaatgtg ccgctgactc cccacggtac gagagaattg acggtcgcac ctgaatccgc
     5521 tagtgttcgt gtcaaatagg tttttgataa gccaacgtca tcaaaagaac gcttgaatgt
     5581 ttctcctgga agaatgatgg acagatactg ttctccaacg atcagattga ttccgataca
     5641 gctcaaggca gtaaacccga ctaaacgaga aggtcggtcc atcttttctt ttaattcttg
     5701 aacgatggta tcaacgatca gatatttgat cagtaaccca cctaatgcca atgcaagtaa
     5761 aatcaaacta gcagaaccta acatactcat gatcccgcct cgagaaagca gttcatctag
     5821 ctttggatca ggtgtatttg cgacataacc attcatcaag atgtctgctg cttttccgac
     5881 cgtcaaactt ttctcatgga taaaagaaag aacgagagca gctgtcgatc ccgctagtaa
     5941 tgtagggata gccggaattt ttttgattgc ccccccaaat aaaataacta gtggaatcaa
     6001 tgtccaagga gagatccaaa agctcgcttt catgttttgg atcatttcat tgatactagc
     6061 tagatcggct gaatgactgt tatgtcctaa tatcgtataa aaaatcaacg aaaggacgaa
     6121 tgctgggaaa tccgtataac gcataccagc gatatgttcg aatagattaa ctcctccaat
     6181 acccgctgcc aagttggtcg tatctgataa aggcgagcag ttattcccta aaaaggcacc
     6241 ggaaacgact gcgcctgtag tcattgcatc attgaatcca agtaaatgac cgatccccaa
     6301 aaaagcaatt ccaatcgtcg aaatcgttgt aaatgaactg ccgacagtca ctccgacaat
     6361 tgcacaaaca agaaatgctg tcggtaaaaa gaaacgcaca gaaacaagtt taaatccata
     6421 aaccataatc gtagggatgg ttccagataa aatccatgaa gcaactaaga caccaatcaa
     6481 aagaaaaatc aggattggaa tgatccctgg agtgattcct ttgatgatcc cgtcatggat
     6541 ctcatcccat gtgaactttt taaatctgcc ataaaacaac aaacacatga aagctgcaag
     6601 gatcgggata tgtggcgaga gctggaatcc gatgatcagt actcctagga gtgccaacaa
     6661 cacgatcaat acagcgatac tttcttttaa agaaatattt ttattcatcg tcgatctcct
     6721 atttcaaatg aaaaggccct gccatgaaaa aactattcac ttggcaggac ctcctactaa
     6781 atcagcgaca aaacgtcggc tgaaagactt atttttttat ttttcttcta aacttttttc
     6841 tacctcaatc ccccaagaat ctttcttgaa gtgattgatg atcaatggaa taacgaacat
     6901 gaccactgtg atagctgcta ctaataatgc atcactcaat gtcgctgtac ctaatgtagc
     6961 aaatacgaca acgatgatag agaagatcag catgcagctg actacccaag ctaatccgtt
     7021 tccgttttta ccaattcggt aaggacgttc gaattctgga tgtttcttac gcattttaag
     7081 caatgaaaca gcaatcaaaa tgtacacgat acaataaata acagttgtag catttgtaag
     7141 tgtcaagaat acagcactta cgtcatccat tacaccataa agcaaagcaa ataatgaaat
     7201 aacgattgat tgtgttagaa cgacatttct agatacgccg tatttgtttt ctttatggaa
     7261 tccgaatttt ggtggaagga atccttcacg agctacttgg atgattgttt ttgatggtcc
     7321 agtaacccaa gctgataatt gtaaaagcac accaataaat accatgaagc tgaagatatt
     7381 tccgatcact tctggtaagc ctaggatttg acagtcaatc aagataggct gcgtgatatt
     7441 tgctaattcc atttttccat ctggtacgac atttgctaca agcatcgcat tgatgacatt
     7501 caataaaact aatccgatca aagcgataaa tacgcctttt gtataatttt ttgttgcgtc
     7561 tttcaaacga ggcatgtaaa cagaactcat ttcgatccca acaaaaataa atgtgatacc
     7621 agctaagtat ttcaatgaat caatatgttc caagtcaggg aaagcttttg accatgaaaa
     7681 gtcacctaga tagccgtttg agactagtcc gactttgaag gctgccaaaa cacctaaaac
     7741 gaacatgatg actactggga tatatacccc tagccataca ccgatgttcc cgccgacttt
     7801 tgccatatcg aatttcaagt tcaagatcgt gatgatccag tagataacta aaatacaacc
     7861 taaaacgaac cagtgattat tgccaagttc tacattacca aatgtgtttc ctaacaatgg
     7921 tcctaaagta gaagcgacca ttaccatacc tgggaacatt tgcacccata atagccatgc
     7981 aacgacaaat ccccatttgc tgcccagtgc tgtttttacc cataactgag gaccgccttc
     8041 ttgaggaagc atcgtggaaa gctcaccgga aaccattgat atcggaccgg caaaaaacaa
     8101 aactgcaaaa attgaataaa aaatcaatgt ccaaccgact gcggctaacg taggaatact
     8161 ccgaaccgtt gcacacagtg ccattgtcag accgataaag gtcccaagac ccattttact
     8221 tacttcagat ttactcaccg ttgacactct ccttgatttt ttggataaaa taacggtgtc
     8281 tttgaagtag tttcagcaat aaacgaaaca attcagcgaa tatttgaggg gattctttga
     8341 gtttattgct tgaagggttt cttcaaagac aacctcaaaa caaagggatc gaagagctta
     8401 ggtctcccct tgctcttcag atccaaacat gcgtcagaaa cagttagtat tcaagctgtt
     8461 ttctaacatg attaccttgc ttattttgct tcgcttgcca ataattggtc tgcgtagatt
     8521 ttttccaatt tttcttgaag agctgctttg attttttcag catattcttc aaagtgttct
     8581 tctttgttca tgtatggtgt cattacgctg gcacgcaata cagttacttt tccggcacgg
     8641 ttccattctt catctgagaa tcctagttgg ttcacaaatt gtaatgggct gttgccatag
     8701 tctgggatag caaagtcagt atgtgaagtt aagaactcat taccgtaaat gcttcctttc
     8761 acgtatgaag aataatcata tacgtcatgg ttcaatttat tcattgcaac taagtcatcg
     8821 ttgccttttt ctttgaatac atagtctacc atattgaaat ctggataagt taatggatga
     8881 acttcgattt ctttgtctcc tactttaaat gacaagtcat tcaagaagtt gtagaaacga
     8941 tgtgcacctt cgattgatgc acccattaat ttaccatatc ctgtgacatt caaaggtaag
     9001 acatggtgtg ctgcccaaac gctggctgct gttgcccctg cttttgaacc ttccaagata
     9061 taagccccaa gtaatgctgg gatatctgcg cctttttcaa atacatatgt cgcaaagtaa
     9121 gagataacgt ctctcatgcg gatatctttg atgacgatac caccggcaga atatggaaca
     9181 tagcccattt tatgtgggtc aatcgttaca gattctgctt cttcgattgc tttatatgca
     9241 ctgtgtactt cttctaagat ataatctttg ttttctgtaa agacattgta tttgtaatga
     9301 acatctttta aatcttcaaa tggaatgaag ttgttgtctt cgtctaagaa aatcgcacgg
     9361 ccgtatccgc cataagcagc atctacgtgt aagtagaagt aaataccgtc tttttctaat
     9421 acgcgacgca atgcaacgat tttatcgata ccgtcgattg ctccttcttc tgttgatcct
     9481 acaacaccga ctacacctag gattggtgtt ttttcagctg ctagaccacg aacgattttt
     9541 tcaagttcgt tgatgtccat gcgatagttg tggtcaactg gcactggaat cacttggtct
     9601 aaaccgatac cgatgatgtc ggcagctttt agccatgaat agtgtttagt ttgtggaacc
     9661 aaccattttc ctaatttttc taaatgtttt ccgctacgtg ctgagtgagc tttgatttcg
     9721 tcgatttttt ctgggacgct gtctaacaga ttcatgattt cttctgttga caagttcatt
     9781 aattcccaat cgctttttcc agcaactagt tcaggtgtca cttctttcat tgcaagcggc
     9841 aatgatttga tgttgcgggc ataccaaagt ccttctaagt ttgctaaaga accatcagca
     9901 acgatgtggc cccagccatc tttatagctc attaattttg caaattccat tcctacttct
     9961 tcttccattt ggcttgttgc aggagaagat tcataggcaa cgttattacc attccatagc
    10021 atagcaaaat tgtacgctag taaagatggc atcaatgttt cagagttcat atgaccccag
    10081 taacgtccgg cattatgcca aggaacggaa tgggtacgca tccgcgctga aatttctgat
    10141 agaacatcct ttgttttatt cacagtgtgt tcaaaactag cactgctttt ttcttctggc
    10201 gtaataatag gcatgtcttg aggcatgtag ttttgacgcc agcctaaatg ttcatccacc
    10261 aattcattca acaaagcttt atagatttgg ccgttctcag ctttgtcccc aatgaatagg
    10321 gcatttaggt ttagatcttt cgacaatgat tcactcatag tgaaaccctc ctaaaaaaat
    10381 atttaaattt tgtcgagccc tacattacac ctgttcttct attctgtaaa ctaagttttg
    10441 gcctgatttt taatcatatt ttaatatgta tcaattatcc gaattgttca cacttttgaa
    10501 ataattaaaa aaaacgaggg aatttacaaa tgtaaactcc ctcgcaaaga ttgtattaac
    10561 gacaaccagc gttctatttt aggatgggtt tcaaaattgg aatgttgtaa tagtatttcc
    10621 ctttttcaag gtaaaaactt actaaattct aatcaattac tttggccaaa aagtaatttt
    10681 tcttaccttt tcgaataacc acaaacttac catcgaattc atcggatgga tttatgacaa
    10741 aatttaaatc ggtaacgcgg tcaccgttaa tacttatagc tccgtttgaa acatcttcac
    10801 gtgcttgacg tttagatggt tcaatttttg tggaaacaag cagttccacg atattttcag
    10861 gtgtgcttga aatttcaacg ttcggcattt taccgaatcc ttgcgcgatt tcttccgcat
    10921 ttagttcttt aatatttcca gagaacaatg cttgcgttat tttttgcgct tctttcaagt
    10981 cttcttcact atgcacaaat cttgtaactt cttctgccaa acggcgttgt gcttcacgtt
    11041 tctcaggttc tgtctctact tttttcgcta agtcttcgat ttcttcttga gacaagaaag
    11101 taaagaattt caagtatttc actacatcgc gatcatcttg attcaaccag aattggtaga
    11161 actcaaatgg tgaagttttc ttcggatcaa gccaaacggc accgcctgca gtttttccga
    11221 atttcgttcc gtcagccttc agcatcaatg ggatcgttaa accgaacgct tctgcttctg
    11281 gtccttctaa ttttcggatt aggtctaatc cagctgtgat atttccccat tggtctgctc
    11341 caccgatttg caattgcaca tcgtatgttt tatgcagatg caaaaagtcg atagattgca
    11401 ggatttggta agtaaactct gtaaatgaga ttcctacttc caaacggctg gcaacgatat
    11461 cttttgctaa catcgtgttg atgttgaagt ttttaccgta gtctctcaaa aattcaagta
    11521 aagagatttt tgacaaccag tcgtagttgt tcacaaatgt gatatttgca tctttaccaa
    11581 acaattttct catttgattg gaaagtgcgt ccacgttatg ttgcacttgt tccatcgttt
    11641 gcaacacacg ttctgttttg cggccgcttg ggtcgccgat cgttcctgtt ccgccgccaa
    11701 tcagaatata tggatgatgg cctgccaatt ggaatctttt catcatcata aatggaatca
    11761 aatgtccgat atgcatcgaa tcaccagttg gatcaacgcc gcaataaagt gaaatgctct
    11821 tttcttcaac gagctcgcga agtctttctt cgtttgtttg ctgattaatg gcgtcgcgcc
    11881 aagttaattc atcaataata ttcattttac tgacctccgt gtagtttttt tatgaaagca
    11941 ggaacctgct agcatatgta attaattatt tcttcaggat aggataggaa ataaaaaaag
    12001 tcccttggca tataagccaa gggacgaaat ctcgcggtac cacccaattt gtatgtctag
    12061 atatccaaca tacctctcga acatttaacg ctgtcgcgtg gcaatcactg ccaaagactc
    12121 ctgaaatgta attcgcttta aagcatgtac tggtttgcag cgaccaccag ctttctgaaa
    12181 cattggcttt aacaactact tcgtttcgat cttcgtcttg ttattattaa tatgtgtcta
    12241 tcatagagcg caactttttc tttgtcaaca gaaaaaggat atttttttta aattcctcat
    12301 tttttctttt ttgaatccgc tctaattccg aacattttat tttcaattat tgttaaaaga
    12361 aataactttt tctgtatttt ttctgcaatt tatcgagaag tcactttact ttgcctctta
    12421 ttctttcttc gttacaataa ggaatgggac aagtaaaaaa atgaaaaagc aagggacgag
    12481 gtatatggaa aaaaagcaaa aaattatttt aggagccact tctgctttaa ctatcgttat
    12541 tttaattcta agtttgtttt ttgtcatccg atcatctaca aaaaaagaac caatcattcc
    12601 tgaaaaaaaa ataacagaaa aagcaaaaga accgacagat gataagcaac aactagttac
    12661 tcaagcaaaa aatctggctc aaagctacga ttatgaccaa gcgattgcgt tattggaaaa
    12721 agatagtaag aacggatcta ctgaaacaca acagcttctt caaacactaa aaaaggagaa
    12781 agaacaactg atcgactggt ctgatcctac tcagatctct catgtctttt tccacagctt
    12841 gatcgtggac ccgcaaaaag ccttccactc tcaacaggca caaggataca aagattatat
    12901 ggtgactgtt gaagaattca accgttcgat cgaacaactt tatcaaaatg attttgtttt
    12961 agttaatttg aatgatttga tccaaaaaga cgaacaagga aatctttcct ttaacgggct
    13021 tgcgctacct gaaggaaaaa aaccattggt tctttcacaa gatgatgtca gctattatga
    13081 atatatggat aattccggtt ttcctagccg attggtcgta tcaaataaca atgaaatcaa
    13141 aaatgtctat aaagagaaag ataaagaaga aatcggtgac tttgacatgg ttcctctgat
    13201 cgatgccttt gtcaagaaac atcctgattt ttcttatcga ggcgcaaaag gtaccttggc
    13261 cctgacaggt tacaacggag ttttaggtta tcgaacatca aaatctcaat atggagacaa
    13321 tgaaaaaaca aaaaaagaga ttaaagaagc taaaaaagtg gcggaacagt tgaagaaaga
    13381 cggctggagc tttgcttccc atacatgggg tcacttgaat atgactcaag cttcactaga
    13441 agatgtaaaa aaagacaatg aattgtggca gcaagaagtt gcacctatcc taggaaaaac
    13501 aaatattctg atttatccat ttggtgcaga catcagcgac tggcagcctt attcagcaga
    13561 aaatcaaaaa tttgcttact tgaagagtca aggtttcgat attttctgta atgtcgatgc
    13621 ttctacacca gcttggggac aattaggtac ggatttctat cgaaatgctc ggatcaatat
    13681 cgatggcatt cgttttcaat ctgatttaga cggaaaaaat cctattcttg atccattcat
    13741 caatgtccat gatgtctatg acaaagaagc acgcgaatct gcttgatttt acgtcattta
    13801 tgaaccaaaa aagccgaact gacattttat cgtcagtttc ggctttttta tgctttattt
    13861 cttcttcggt gcgattccct tgatcaggac ttctgtccat tctgctgtat atggaagcag
    13921 ttttgcgtat atctccggct tagaaattcc ttctaccatt gcttgaaaat agaggatcaa
    13981 gaactcatcc gaatatttca aatccacttt tccttctttc cgccctcgat ggaaaagatc
    14041 aagcatgata ctgaaacttt cttgcgtata cgcatccatc gcgtcagaaa attctttttt
    14101 ttcttttttt gtatagtatg tcatcaaatc gatataaaat tgtttgttca ctcgattgag
    14161 ataacggatt ttgttttcac tcatggcaat cagtgtttct tcaaatggaa gattttccgc
    14221 aatcaacgct ttcgcttctt gccctaaatc attcataaat gcaaaaaata cttcataaat
    14281 cagcccttcc ttgctgccaa aatatttaaa aatcgtcgct ttacttacct gagcgttctc
    14341 agctacctta tccattgtca ctccagcggt tcctatgtct gtgttcatca agtcaaaagc
    14401 tgcttgtaaa atctctttct ttttctcttg tgctctcttt tcaaaaccgt tcatttgtag
    14461 agcctcccgt aatt
//
LOCUS       DACCXO010000067        14181 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.068, whole genome shotgun sequence.
ACCESSION   DACCXO010000067 DACCXO010000000
VERSION     DACCXO010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.068"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..475
                     /locus_tag="IVY70_001828"
     CDS             <1..475
                     /locus_tag="IVY70_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7958955.1"
                     /translation="LEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALN
                     QWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILR
                     RNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            981..1583
                     /locus_tag="IVY70_001829"
     CDS             981..1583
                     /locus_tag="IVY70_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7958956.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1599..2276
                     /locus_tag="IVY70_001830"
                     /pseudo
     CDS             1599..2276
                     /locus_tag="IVY70_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2788..3084
                     /locus_tag="IVY70_001831"
     CDS             2788..3084
                     /locus_tag="IVY70_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HAP7958957.1"
                     /translation="MSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTNSASGVLRAN
                     EIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLKEKVVR"
     gene            3085..3501
                     /locus_tag="IVY70_001832"
     CDS             3085..3501
                     /locus_tag="IVY70_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP7958958.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            3521..5065
                     /locus_tag="IVY70_001833"
     CDS             3521..5065
                     /locus_tag="IVY70_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7958959.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            5208..5432
                     /locus_tag="IVY70_001834"
     CDS             5208..5432
                     /locus_tag="IVY70_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7958960.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            5447..6316
                     /locus_tag="IVY70_001835"
     CDS             5447..6316
                     /locus_tag="IVY70_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP7958961.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            6297..7031
                     /locus_tag="IVY70_001836"
     CDS             6297..7031
                     /locus_tag="IVY70_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7958962.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGVADEMRRPMMLIVSAKKKM"
     gene            7064..7972
                     /locus_tag="IVY70_001837"
     CDS             7064..7972
                     /locus_tag="IVY70_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP7958963.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7969..8511
                     /gene="sat4"
                     /locus_tag="IVY70_001838"
     CDS             7969..8511
                     /gene="sat4"
                     /locus_tag="IVY70_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAP7958964.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            8604..9398
                     /locus_tag="IVY70_001839"
                     /note="aph(3')-IIIa"
     CDS             8604..9398
                     /locus_tag="IVY70_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP7958965.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(9506..9694)
                     /locus_tag="IVY70_001840"
                     /pseudo
     CDS             complement(9506..9694)
                     /locus_tag="IVY70_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9674..10246
                     /locus_tag="IVY70_001841"
     CDS             9674..10246
                     /locus_tag="IVY70_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7958966.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
     gene            10586..11440
                     /locus_tag="IVY70_001842"
     CDS             10586..11440
                     /locus_tag="IVY70_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7958967.1"
                     /translation="MEKEKLKILEELRRILNNKNEAIIILNNYFKGGVGKSKLSTMFA
                     YLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKNGNLASSIIHLTDNL
                     DLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIIIDTVPTPSVYTNNAI
                     VASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFVPYLVDTDSATIKSN
                     LEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVLSMYENVFFEMIERI
                     IQLENEKE"
     gene            11539..11748
                     /locus_tag="IVY70_001843"
     CDS             11539..11748
                     /locus_tag="IVY70_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP7958968.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            11765..12037
                     /locus_tag="IVY70_001844"
     CDS             11765..12037
                     /locus_tag="IVY70_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP7958969.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            12039..12902
                     /locus_tag="IVY70_001845"
     CDS             12039..12902
                     /locus_tag="IVY70_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP7958970.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(13165..13902)
                     /gene="erm(B)"
                     /locus_tag="IVY70_001846"
     CDS             complement(13165..13902)
                     /gene="erm(B)"
                     /locus_tag="IVY70_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP7958971.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(14027..14110)
                     /locus_tag="IVY70_001847"
     CDS             complement(14027..14110)
                     /locus_tag="IVY70_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP7958972.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
BASE COUNT         5015 a         2221 c         2928 g         4017 t
ORIGIN      
        1 ccttgaaggt gatacgatcg ttggcattca tcataaaagt gccgtcatta ctttagttga
       61 aagattatct aaagtcatta tcacgattaa acccaacggc cgtaaggcat tagatattga
      121 aactgccctt aatcaatggt tttctcgctt ccctaaaaac ttctttaaat ctattacgtt
      181 tgactgtgga aaagaatttt ctaactggaa agccattagt aaccaacatg atattgatat
      241 atattttgcg gaccctggaa caccttctca acgcccatta aacgagaatt ctaacgggat
      301 tctgcgtcgt aatggactgc cgaaatcaat ggattttaga aaagtgaatc agacatttat
      361 ttccagtgtc agcaatcaac gtaatcatat tccaagaaaa tcattgaatt acagaacacc
      421 aatcgaaata tttttgagct atgtacaaga agcattttat tctagcttaa tttgacaaat
      481 catactatga tacgtgtttg tttttctgtg ctgtttagtg aatgattagc agaaatatag
      541 agagtaagat tttaattaat tattaggggg agaaggagag agtagcccga aaacttttag
      601 ttggcttgga ctgaacgaag tgagggaaag gctactaaaa cgtcgagggg cagtgagagc
      661 gaagcgaaca cttgatcttt taagttgcta tctttttaag gtcaatagag tatacttatt
      721 tgtcctaata tggttttagc agtataattg acttggtgaa taggtcattt aagttgggca
      781 taataggagg agtaaaatga aaaaatttat ttatcgagtt ttagaaaatg acgaagtggt
      841 ggctattttt aatgagcaac aatatgcgca agattttatc gcttacgaaa agacaatttc
      901 tgataagcaa tttgaaattg aaaaagtaga tatagctgat tggttattgc aaccgagaga
      961 attttagagg ttggttgaaa atggctaaaa ttggttatgc acgtgtcagt agcaaagaac
     1021 agaacttaga tagacaactg aaagcgttag agggcgtttc taaggtcttt tcagacaaat
     1081 taagcggtca atcggtcgaa cgcccacaat tacaagctat gcttaactat attcgtgaag
     1141 gggatatagt tgttgttact gaattagatc gattaggacg aaataataaa gaattaacag
     1201 aattgatgaa tcaaattcaa attaaggggg caaccctgga agtcttaaat ttaccctcaa
     1261 tgaatggtat tgaagatgaa aatttaagac ggctgattaa taatttagtg attgaattgt
     1321 ataagtacca agcggaatct gaacgcaaac gaattaaaga acgccaagcc caaggaattg
     1381 aaattgctaa gaaaaaagga aaattcaaag ggcgacaacc taaattcaaa gaaaatgatc
     1441 cacgtttaca acacgctttc gatttatttt tgaatggttg ttccgataaa gaagttgaag
     1501 aacaaactgg aattaatcgc cgaacgttta gaaggtatcg atcaagatac aacgtgacag
     1561 tagatcaaag aaaaaaacaa tgaaaagagg gatagttaat gagtacggtt attttagctg
     1621 aaaaaccaag ccaggcatta gcctacgcaa gtgctttaaa acaaagcacc aaaaaagacg
     1681 gttattttga gatcaaagac ccactattta cagatgaaac gtttatcacc tttggttttg
     1741 ggcatttagt cgaattagca gaaccaggtc attataacga aaagtggcag aactggaaac
     1801 ttgaatcttt gccgattttt cctgatcgat acgattttga agttgcaaaa gataagggaa
     1861 agcagtttaa aattgttgct gaacttctca aaaaggcaaa tacaattatt gtcgcaacag
     1921 atagcgacag agaaggtgaa aatatcgcct ggtcgattat ccataaagca aatgcctttt
     1981 ctaaagataa aacgtataaa agattatgga tcaatagctt agaaaaagat gtgatccgaa
     2041 gcggttttca aaatttgcaa ccaggaatga attactatcc cttttatcaa gaagcgcaaa
     2101 cacgccaaat tgccgattgg ttgatcggca tgaacgcaag ccctttatat accttgaatt
     2161 tacaacaaaa aggcgtacaa ggtacatttt cattaggacg tgtacaaaca ccgactttat
     2221 atctcattta tcaacgccag gaagcaatag aaaactttaa aaaagaacct tttttcctga
     2281 ataattcata gttttactaa tagtctttcc taacattgat ttaatagtct ttaaagtatt
     2341 ctgcttcatc acccattagg tgatgaaaaa agaacccctt tctgatttag cggacaaata
     2401 ctttgaccta atctcagaag atataaaaga aaaaataata aaatatccga tttatacttt
     2461 gtataaagaa ctaaagtagt tagcaaatca tttacagcaa atcactatgt tccacatacc
     2521 attttacata cttaccttat ccacgacaca tgttgagata gtatcgaaga taaagaaagt
     2581 tgctccatgt tgtttttgcg agatggggaa atcaaagcca cattgacaac catgatgaat
     2641 aagcttgctt tcagtcataa gctaatccta gaaccacttt tcaagtcagt tagccaaatc
     2701 gatgaagaaa gcgaccgtga aagaatggac gctattgata agctaatgga gcaactattg
     2761 gaagaacgca atacccttat tgcactgatg tccaaaggtt tcctagaacc tgctcttttt
     2821 aatcaggaac gaaatgtttt agatagtgag attaaaaatc ttactaccga aaaaacaaac
     2881 ctggtaacga actctgcgag tggggttttg cgagcaaacg agataaagga cctcattaat
     2941 tatgtgtcag cagataattt taatggtgac tacacggaag aactatttga agaatttgta
     3001 gtgaacatca ttgtaaattc cagggatgag ctgacattca atttgaaatg cggtctttcc
     3061 ctgaaagaaa aggtggtgag ataaatggca tatattccat atggatacaa aattcaagat
     3121 ggagtggtta ctgtcgatga aaaggcagca ggtcaagtaa aggtattctt tgagaaatac
     3181 atatcaggac tatcccttac agtggctggc gaacaggcag gtattgaaaa gacacattct
     3241 gtgatggggc gcattttgaa aaacgtcaac taccttggaa atgatacgta tccagcaatt
     3301 attgataaag agatatttga taaagctgaa gaagttagag ataaacgtgc aaaggattta
     3361 ggacgagtgg tagagcttgc cgctttcacc tctccccctc ccaaagaacg attcaaaatg
     3421 aaaaaggcag ataataagat gccagtggat ccttttgaac gagcagaata cttatacagt
     3481 ctgatagaaa gcgaggaata aagtgacaga gaaaaatata atggttattc ctgctcgtaa
     3541 aagagtagga agtacagccg caaaagaaaa gataaagaaa cttcgtgttg ctgcctattg
     3601 ccgtgtttct acagaaacag aagaacaaaa ttctagctat gaggttcagg tctcacacta
     3661 tacagagttt ataaagaaaa ataatgaatg ggagtttgct ggcatctttg cagatgatgg
     3721 tatctctggt acgaacacta aaaagcgtga cgaatttaat cgcatgattg cagagtgtat
     3781 ggacggtaac atcgacatgg ttattacaaa atccatcagc cgatttgcgc gtaacaccct
     3841 agactgcctt caatacatta gacagctcaa ggataagaac atatcagttt attttgagaa
     3901 agagaacatc aacaccacgg atgccaaagg tgaggttttg ctgactatta tggcatcttt
     3961 ggcacagcag gaaagccaga gcctttcaca aaacgttaag cttgggctac agtaccgata
     4021 ccaacaagga aaggtgcagg tcaaccataa gcgatttatg ggctacacga aagatgaaga
     4081 tggaaattta actattgtcc ccgaagaagc tgagattatc aaacgcatct accgagaata
     4141 ccttgagggt cagagtctag tgggcattgg tcgagccctt gaaaaggatg gtattttaac
     4201 agcagcggga aaaccaagat ggcgaccaga atcagttaag aagatacttc aaaacgaaaa
     4261 atacatcgga gatgcccttc tgcaaaagac tgtcacagta gattttctga ccaagaaacg
     4321 agtgaaaaat gaagggcatg ttccccaata ttatgttgaa aatagccatg aagcgattat
     4381 tcctaaagag ttatttttgc aggcgcaaga agaaattcat cggagaagca atatctacac
     4441 aggagaaggc aagaacaaaa gaatttacag tagcaagtac gctttaagtg ccatcacctt
     4501 ctgtggagat tgtggcgata tttatagacg ggtctattgg aatatccacg gaagaaaaga
     4561 atttgtctgg cgatgcgtga ctagaatcga gcaaggtcct gaagtttgta agaaccgaac
     4621 cgtaaaagaa gatgaacttt atggtgcagt aatgactgcg attaataagc tacttgcagg
     4681 tgggaacaat atgataaaga cactggaaga gaatattcat gctgtgattg gcgaaaccac
     4741 agaataccaa atttcagaga ttaacaactc actagaggaa aaacaaaaag aactaatcaa
     4801 gctggcgaac aagggtcaag actatgacca cttagcagat gagattgatg agctgagaga
     4861 caagcgacag atccttttag tagaagatgc ctccctcagt ggcgagaacg agcgaatcaa
     4921 tgagctgatt gaatttatcc gcaagaacaa attccgcacc ttagaatatg atgataagct
     4981 tgtaaggaag ataatccaga gcgttacagt ctatgaaggc cacttcgtca tagccttcaa
     5041 atctggcatc gaaatggaaa tatgaaaacc agaagtaaat agcccatgac tctacagtag
     5101 agttgtgggt tttcttttgc ttgctgatta taataatatt ttaataacta ttgtgtttat
     5161 caaccaaatg gtttataata ttcttagcgt aaacttggag gtgctgtatg actacggcag
     5221 aaatgattaa agaactgtgt gagcaaatga atataagtgt ttccgaactt gctagacgta
     5281 ttggccagac tccacagaat ttcaataaaa aattacaacg agaaacagta accttggatg
     5341 agttgaaggc cattgctgat gtgctaggtg tcaagtttgt gcaggcattt attttaccag
     5401 atggagaaga aataaaaata tctaacgaat aaaggaggat gtttatatgg taggtaatgt
     5461 tattcaaata gtaacagaga aattatcatc tttgcctttt atagaaggca tcgtattagg
     5521 gggctcacgt gcaagaggca cccatacaga aaattctgat atagatatcg gaatctatta
     5581 caattcagac tcatttgacc tgacagcgat taatcaaatt gctacagagt tggatgatga
     5641 gaatagaaac aaccttgttg tacctcccgg agcgtggggt gattgggtta atggcggtgg
     5701 atggttagtt ataaacgggt gtcatgttga cttgatatta cgtgatataa aacgggtgga
     5761 acaaataatc aaagatacgg agcaaggaat tgttactgcc aattatcaga ccgggcatcc
     5821 ccatggttat ataagtgcta tgtatcgtgg agaattagcg attagtaaaa tactatatgc
     5881 taagaacgaa agcttatgcg aattaaaaaa gcaggcagaa atttacccta ctgctctaaa
     5941 gaaaagcttg atgaactttt ttatatttga agctgaattc tctttaatgt ttgtaaaagc
     6001 aaatgcgggg gcagaggata aatattatat tgcaggccat gtttttcgta ttatttcatg
     6061 cttaaatcaa gtactatttg catgtaataa tgcttattgc attaacgaaa agaaagctat
     6121 aaaactgctt gaaacttttg aatataaacc tgaaaaatat gccgaaagag ttaatcatat
     6181 ttttgaagta ctcggtcttt ctctttttga atgctacgac atgaccgaga aactttataa
     6241 agaagtgaat gaaattgtat cggagataaa taacttttta aacgaggaga gttcagatga
     6301 aagaaaacaa atatgatgat aatatatttt ttcaaaaata cagtcaaatg agtcgctcgc
     6361 agaaaggact ggctggtgcg ggagaatggg agactttgaa aaagatgcta cctgatttta
     6421 agggtaagcg tgtgcttgat ttaggatgcg gctatggatg gcactgtata tatgcgatgg
     6481 aaaacggtgc ttcctctgta gtaggtgttg atatttctca taaaatgctc gaagtagcaa
     6541 aaggaaaaac ccattttcca cagattgaat atgaatgctg tgccatagaa gatgtggatt
     6601 tcccagagga gagctttgat gtaatactaa gttcgcttgc gtttcattat gtagcagact
     6661 atgagaattt aataaaaaag atatatagga tgctgaaggc tggtggcaat ttagttttta
     6721 cagttgaaca tcctgttttt actgctcatg gaacacaaga ctggtattat aacgaaaaag
     6781 gagaaatact gcatttcccg gtggacaatt attattatga gggcaaacgg acagctatgt
     6841 ttttggaaga aaaggttaca aaatatcata gaacactgac cacatatcta aatacactgc
     6901 tttcaaatag ttttataata aatcagattg tggagccaca gccgccagag aacatgatgg
     6961 atattccggg ggtggcggat gaaatgcgac gcccaatgat gctgattgta tcggcaaaaa
     7021 agaagatgta ataatataga aaaaataaac gaggagtatg taaatgagat cagaaaaaga
     7081 aatgatggat ttagtacttt ctttagcaga acaggatgaa cgtattcgaa ttgtgaccct
     7141 tgaggggtca cgcgcaaata ttaatatacc taaagatgaa tttcaggatt atgatattac
     7201 atattttgta agtgatatag aaccgtttat atctaatgat gactggctta atcaatttgg
     7261 gaatataata atgatgcaaa agccggagga tatggaatta ttcccacctg aagaaaaggg
     7321 attttcctat cttatgctat ttgatgatta caataaaatt gatcttacct tattgccctt
     7381 ggaagagtta gataattacc taaagggcga taaattaata aaggttctaa ttgataaaga
     7441 ttgtagaatt aaaagggaca tagttccgac tgatatagat tatcatgtaa gaaagccaag
     7501 cgcaagggag tatgatgatt gctgcaatga attttggaat gtaacacctt atgttattaa
     7561 aggattgtgc cgtaaggaaa ttttatttgc tattgatcat tttaatcaga ttgttcgcca
     7621 tgagctgctg agaatgatat catggaaggt cggcatcgaa acaggcttta aattaagtgt
     7681 aggcaagaac tataagttta ttgaaaggta tatatccgag gatttgtggg agaaactttt
     7741 gtccacctac cggatggatt cctatgaaaa catatgggaa gcattatttc tatgccatca
     7801 attgttcagg gcggtatccg gtgaggtggc ggaaaggctt cattatgcct atccggagta
     7861 tgataggaat ataacaaaat ataccaggga catgtataaa aaatacactg gtaaaaccgg
     7921 ctgcctggat agcacatatg ccgctgatat agaagagagg cgggaacagt gattacagaa
     7981 atgaaagcag ggcacctgaa agatatcgat aaacccagcg aaccatttga ggtgataggt
     8041 aagattatac cgaggtatga aaacgagaat tggaccttta cagaattgct ctatgaagcg
     8101 ccatatttaa aaagctacca agacgaagag gatgaagagg atgaggaggc agattgcctt
     8161 gaatatattg acaatactga taagataata tatctttact accaagacga taaatgcgtc
     8221 ggaaaagtta aactgcgaaa aaattggaac cggtacgctt atatagaaga tatcgccgta
     8281 tgtaaggatt tcagggggca aggcataggc agcgcgctta tcaatatatc tatagaatgg
     8341 gcaaagcata aaaacttgca tggactaatg cttgaaaccc aggacaataa ccttatagct
     8401 tgtaaattct atcataattg tggtttcaaa atcggctccg tcgatactat gttatacgcc
     8461 aactttgaaa acaactttga aaaagctgtt ttctggtatt taaggtttta gaatgcaagg
     8521 aacagtgaat tggagttcgt cttgttataa ttagcttctt ggggtatctt taaatactgt
     8581 agaaaagagg aaggaaataa taaatggcta aaatgagaat atcaccggaa ttgaaaaaac
     8641 tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata
     8701 agctggtggg agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga
     8761 ccacctatga tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg
     8821 ttccaaaggt cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg
     8881 ccgatggcgt cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg
     8941 agctgtatgc ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata
     9001 cgaatagctt agacagccgc ttagccgaat tggattactt actgaataac gatctggccg
     9061 atgtggattg cgaaaactgg gaagaagaca ctccatttaa agatccgcgc gagctgtatg
     9121 attttttaaa gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag
     9181 acagcaacat ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg
     9241 gcagggcgga caagtggtat gacattgcct tctgcgtccg gtcgatcagg gaggatatcg
     9301 gggaagaaca gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga
     9361 aaataaaata ttatatttta ctggatgaat tgttttagta cctagattta gatgtctaaa
     9421 aagctttaac tacaagcttt ttagacatct aatcttttct gaagtacatc cgcaactgtc
     9481 catactctga tgttttatat cttttctaaa agttcgctag atagagttct atattagaga
     9541 tgtaaagagt tttggtgaag aggtttgtga gtatggcttt tataaccagg gtgcaatgag
     9601 aagcactaac agcagctagc ttaagaacgc ataggatata ctttttatgg aagtcacaga
     9661 ggaggaataa aatttgaaca ggattaacag agtaacctct attcttattc agctgcaatc
     9721 aaaaaaaata attcctgcca aagaaattgc acagcgattt aatataagct taaggacagt
     9781 ttacagagat atccggacac ttgaagaagc cggaatacca attggatctg aggccggaaa
     9841 aggatatttt cttgtagagg gcttcctact tccgccggta atgtttacag cggcggaagt
     9901 gggtgcattg attacagcag ggaaattttt aaattgccat ggagatgaat catttataaa
     9961 ggattttgat tcagctatgt ataaaatcaa atctattttg aagcatggtg aaaaaaacta
    10021 tgcacaggag ttggagaaca gtattaacgt gtacagcaca tctggacaga aaaatacatt
    10081 ggcggataac gttattgcag caattcagac tgcaatctgc aataaaaggg taatatcaat
    10141 tcaatatcct gcatcaggag ggcaagaacc ggaaagcaga atgatagagc ctatatcact
    10201 ggggttttat gaacagaact ggtacttaat cggttttgca ggctaagaaa tgaatatagg
    10261 aattttcgtg tggatcggat taaaagcatt tgcatagaag aagagctctg tcaaagccat
    10321 gatggctctt tggaacaaat attaaaacaa atgctttcat ataaaaaact atatgcccta
    10381 caatgccgtt gtaaaaattg gggaaaaggg atatattgat tttatatctt tctcaaaata
    10441 aatataaaag ccctttaaag agggctttta tatattaatc acaaatcact tatcacaaat
    10501 cacaagtgat taatcacaaa tcacttgtga tttgtgattc ttaatgatac aatattacta
    10561 tacaaaaaaa gaatggggcg tagttatgga gaaggaaaaa ctaaaaatac ttgaagaatt
    10621 aagacgtatt ttaaacaata aaaatgaagc aattattatc ttgaataatt actttaaagg
    10681 tggtgttgga aagtccaaat tatcgactat gtttgcttac ttgacagaca aattgaattt
    10741 aaaagtttta atgatcgata aggacttaca ggcaacattg acaaaagact tagcaaaaac
    10801 ttttgaggta gaattgccac gtgtcaattt ttatgaaggc ttgaaaaatg gaaacttggc
    10861 ttcttctatt attcatttga ctgataattt agacttgatc cctggcacgt ttgatttgat
    10921 gttactgcca aaattaactc gctcatggac gtttgaaaat gaaagtagat tgcttgctac
    10981 tcttttagca cctttaaaaa gtgactatga tcttattatt attgatactg taccaacgcc
    11041 aagcgtttat acaaataatg caatcgtggc gagtgattac gttatgatcc ctttacaagc
    11101 agaagaagaa agtacaaaca acattcaaaa ctatatttcc tatttgattg atttgcaaga
    11161 acagtttaac cctggactag atatgattgg ttttgttcct tatttagttg atacggacag
    11221 cgcaacgata aaatcaaacc tggaagaact gtacaaagaa cataaagagg ataatttggt
    11281 tttccgaaat attatcaagc gaagtaataa agtaagtact tggtctaaaa atggcattac
    11341 agaacacaaa ggctatgaca aaaaagtttt gtctatgtat gagaacgtat tttttgaaat
    11401 gattgagcga atcattcaat tagaaaatga aaaagaatag aatcacaaat cacaagtgat
    11461 taatcacaaa tcacttgtga tttgtgatag gtgatgataa aataaatagt aagaagaaat
    11521 agaaagaagt gagcgatcgt gggaaattta ggcgcacaga aagcaaaacg aaatgatacg
    11581 ccaatcagcg caaaaaaaga tataatgggg gataagacgg ttcgtgttcg tgccgacttg
    11641 caccatatca taaaaatcga aacagcaaag aatggcggaa acgtaaaaga agttatggat
    11701 caagccttag aagaatatat acggaaatat ttacctgaca aactttaaaa ggagtgaaaa
    11761 tgaaatggca gttacgtatg aaaaaacatt tgaaattgaa atcattaatg agttatcggc
    11821 aagtgtttat aatcgggtat taaattatgt tttgaatcat gaactagata ctaaaaatac
    11881 tcgtttacta gaagtgaatc ttttaaatca attagaagtg gcacaagaag ttgatttatt
    11941 tcaacaacca tttgaagaat tacaagctat tcatgagtat tggcggtcaa tgaatcaata
    12001 ttcaaaacaa attttgaata aagagaaagt ggcttaacat ggcgaatatt actgacttca
    12061 ccgaaaagca atttgaagat cgtttagaaa agaatgttga acgactaact aaaaatagac
    12121 tagcggttga atcgccaacc gcttttttac ttggtgggca accagggtca gggaaaacca
    12181 gtttgcgatc agcaattttt gaagaaacac aagggaatgt tattgtcatt gataatgata
    12241 cctttaaaca acagcaccca aattttgacg aattagtgaa actctatgaa aaagacgtag
    12301 taaaacacgt taccccttat tcgaatcgca tgacagaagc acttattagc cgtttaagcg
    12361 atcaaggcta taatttagtg attgaaggaa caggacgaac aacagacgtt cccattcaaa
    12421 cagccacaat gcttcaatct aaaggttatg aaacaaaaat atacgtcatg gcagtaccga
    12481 aaattgaatc atacttagga acgattgaac gatatgaaac catgtatgca gatgatccaa
    12541 tgacagctag ggcaacacca aaacaagcgc atgatattgt tgtcaaaaac ttaccgacca
    12601 atttagaaac ccttcataaa acgggcttat ttagcgatat aaggctttac aatagagaag
    12661 gagtgaaact ctattcaagc ttagaaacgc cttccattag tccgaaagag accttagaaa
    12721 gagaattaaa tcgtaaagta tcagggaaag aaattcaacc gactttggag agaatagagc
    12781 aaaaaatggt tcaaaatcaa caccaagaaa cccctgaatt taaagcaatt caacaaaaaa
    12841 tggaaagctt gcagctacct acaccaccaa tacccaaaac acctaaactt ccaggtattt
    12901 aaaccttaaa aaaggaaaag agtagttacc aaaaaacggt aactactctt tttttataaa
    12961 aacatttcct catgttgggg tcccgagcgc ctacgaggaa tttgtatcga taagaaatag
    13021 atttaaaaat ttcgctgttt ttttctaatt tcattttgag gtgaattaat tggtagttat
    13081 tagaggtgcc ctatacctaa taatttatct acattccctt tagtaacgtg taactttcca
    13141 aatttacaaa agcgactcat agaattattt cctcccgtta aataatagat aactattaaa
    13201 aatagacaat acttgctcat aagtaacggt acttaaattg tttactttgg cgtgtttcat
    13261 tgcttgatga aactgatttt tagtaaacag ttgacgatat tctcgattga cccattttga
    13321 aacaaagtac gtatatagct tccaatattt atctggaaca tctgtggtat ggcgggtaag
    13381 ttttattaag acactgttta cttttggttt aggatgaaag cattccgctg gcagcttaag
    13441 caattgctga atcgagactt gagtgtgcaa gagcaaccct agtgttcggt gaatatccaa
    13501 ggtacgcttg tagaatcctt cttcaacaat cagatagatg tcagacgcat ggctttcaaa
    13561 aaccactttt ttaataattt gtgtgcttaa atggtaagga atattcccaa caattttata
    13621 cctctgtttg ttagggaatt gaaactgtag aatatcttgg tgaattaaag tgacacgagt
    13681 gttcagtttt aatttttctg acgataagtt gaatagatga ctgtctaatt caatagacgt
    13741 tacctgttta cttattttag ccagtttcgt cgttaaatgc cctttacctg ttccaatttc
    13801 gtaaacggta tcggtttctt ttaaattcaa ttgttttatt atttggttga gtactttttc
    13861 actcgttaaa aagttttgag aatattttat atttttgttc atgtaatcac tcctgaagtg
    13921 attacatcta taaataaata cagaagttaa acgatttgtt tgtaatttta gttatctgtt
    13981 taaaaagtca taagattagt cactggtagg aattaatcta acgtatttat ttatctgcgt
    14041 aatcactgtt tttagtctgt ttcaaaacgg tagatgtttt atctacatta cgcatttgga
    14101 ataccaacat gacgaatccc tccttcttaa ttacaaattt ttagcatcta atttaacttc
    14161 aattcctatt atacaaaatt t
//
LOCUS       DACCXO010000068        13969 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.069, whole genome shotgun sequence.
ACCESSION   DACCXO010000068 DACCXO010000000
VERSION     DACCXO010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.069"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(325..2322)
                     /gene="tkt"
                     /locus_tag="IVY70_001848"
     CDS             complement(325..2322)
                     /gene="tkt"
                     /locus_tag="IVY70_001848"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP7958973.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPIGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2424..2675)
                     /locus_tag="IVY70_001849"
     CDS             complement(2424..2675)
                     /locus_tag="IVY70_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP7958974.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2822..3445
                     /gene="lexA"
                     /locus_tag="IVY70_001850"
     CDS             2822..3445
                     /gene="lexA"
                     /locus_tag="IVY70_001850"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP7958975.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3624..3842
                     /locus_tag="IVY70_001851"
     CDS             3624..3842
                     /locus_tag="IVY70_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958976.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4130..4642)
                     /locus_tag="IVY70_001852"
     CDS             complement(4130..4642)
                     /locus_tag="IVY70_001852"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP7958977.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4656..6953)
                     /gene="recJ"
                     /locus_tag="IVY70_001853"
     CDS             complement(4656..6953)
                     /gene="recJ"
                     /locus_tag="IVY70_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP7958978.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLMHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPVE
                     FLDLVAIGTIADLVSLTDENRILVSLGINAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSN
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     IMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7095..7529)
                     /locus_tag="IVY70_001854"
     CDS             complement(7095..7529)
                     /locus_tag="IVY70_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP7958979.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7539..8327)
                     /locus_tag="IVY70_001855"
     CDS             complement(7539..8327)
                     /locus_tag="IVY70_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7958980.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETDFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8324..9265)
                     /locus_tag="IVY70_001856"
     CDS             complement(8324..9265)
                     /locus_tag="IVY70_001856"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP7958981.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9442..10029)
                     /locus_tag="IVY70_001857"
     CDS             complement(9442..10029)
                     /locus_tag="IVY70_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP7958982.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10250..11563)
                     /gene="obgE"
                     /locus_tag="IVY70_001858"
     CDS             complement(10250..11563)
                     /gene="obgE"
                     /locus_tag="IVY70_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP7958983.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11851..12861
                     /gene="gap"
                     /locus_tag="IVY70_001859"
     CDS             11851..12861
                     /gene="gap"
                     /locus_tag="IVY70_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP7958984.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12946..13881)
                     /locus_tag="IVY70_001860"
     CDS             complement(12946..13881)
                     /locus_tag="IVY70_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7958985.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3856 a         3130 c         2455 g         4528 t
ORIGIN      
        1 gagcaatcgc ttttttattg atcaccattt ttcttttagg tctatggtgt cgggcactgc
       61 gggtcttcag ctcttgcatt caaattcctc ctcaaaataa aaaaacttaa taaaaagttc
      121 tcttcttaaa acaaacttaa ttaattaaat tatactttta attctcaaga aacacaaact
      181 aatccgaaga attctttgtt ttgtcattta aatgtaacat agccgaaaac gaaaagtaat
      241 attgatttat tcttctaaca cataaagaac agctgtctcc aacttgcaga cagctgttct
      301 tatcagtcaa acaattgctt ggatctatga tagttgattg tacgtattca cgacattttc
      361 tactgtaaat ccaaattctt ctaaaatttt agtgccaggg gcagatgcac cgaaatgatc
      421 gattgtaatc gttgcgccct ctgttcctac gtagcgttcc caaccaaatg atgcagcagc
      481 ttcgatagcc acacgtttct tcactgattt aggaagtaca ctttctttat attcagaaga
      541 ttgtttttca aagaggtcaa agcttggcat agaaacaaca gaaacatctt tgcctttttc
      601 agccaattct ttttgagctt tcacagccaa atcaacttca gaacctgttg caatcaaaat
      661 accttctggt gtttctcctt gtgatggaga aagaacatac gcgccttttt tcaccatttc
      721 atctgctact tcttttgtag aaggcaatac tggaaggttt tgacgactca atacaaggat
      781 agtaggcgca tctgttgtct ccatcgctac tttccaagca gcacgtgttt catttccgtc
      841 tgccggacga atcacttgaa caccaggcat gcagcgaaca gatgctagtt gttcaatcgg
      901 ttcgtgcgtt ggtccgtctt ctccgacagc aaccgagtca tgtgttaaga cgtaaacaac
      961 aggagtatgt tggattgctg ctaaacgaac tgctggtctt agataatcga caaagacaaa
     1021 gaatgttcca ccgtagatac gagttccgcc atgaagttgg ataccattca tagccgaagc
     1081 cattgcaaat tcgcggacac cgaaccagat gttgcgtcct tcataatgtt caggagtgaa
     1141 atctttatct gcagccacca ttgtattatt ggaacctgac aagtccgctg aaccacccca
     1201 gaagcttggt atagctttcg ataattcttg gatgacttct ttacttgaaa cacggcttgc
     1261 ttgactgctt ccaacttcat aagttggcaa ttctgcatcc cagttttctg gtagtttgcc
     1321 atcaaaagca tcttcaaatt gttgtgctaa ttcaggataa gcttttttat aattggcaaa
     1381 catttcattc catgcatctt cagctttttg tccttcttct atcatcgttt gatggaagcg
     1441 ggcagctact tcttctggaa cagtgaaatc tggatactcc caaccataaa cttctttagc
     1501 catcttgatg ccatcttcac caataggagc accatgaaca gcagatgttc cttctttagg
     1561 agcaccatag ccgatgactg tttttacttc gatcaatgtt ggtttgtctg tttcagcttt
     1621 tgcttcttca atcgctttag agattgcttc taggtcattt ccgtctttca ctaagatatg
     1681 ttgccagcca tatgcttcat agcgagcacc aacgttttct gtgaaagctt ttgaagttgg
     1741 accgtctagt gaaatatcgt ttgaatcgta taaaacgatc aatttcccta gtttcatatg
     1801 acctgccatc gaacttgctt cttgagatac cccttccatc aagtctccgt caccgcatat
     1861 tgcatacgta tagtgatcca tcacattaaa gttttctttg ttatacgttg ctgcaaggtg
     1921 cgcttctgcc attgccatac cgacagccat tgcaatccct tgtcctaatg gccctgtagt
     1981 ggcttctaca ccatctgtgt gatgtacttc aggatgtcct ggtgttttag aatcccattg
     2041 acggaattgt ttcaaatcat cgattgtcac ttgatagcca gctaaatgaa gcaagctata
     2101 caacattgct gacccatgtc ctgccgaaag aacgaaacgg tcgcggtcag cccagttttt
     2161 tgatgtcgtt ggatttactt tcaggtgttt tgtccataac gcatatgcca taggtgctgc
     2221 gcccatcggc aatccaggat gtcctgagtt tgctttttgg actgcttcaa tacttaatgt
     2281 acgaattgtg ttaacgccaa gttggtcaat tttgtcaaac aaaataaccc ctcctattaa
     2341 tttaggtttt ttttaccttt atttcttaca ctttcattct ataagatgtc acacgaaaaa
     2401 gcaatctttt tcagtatttt ttactaaaag ttgtcatttc ggttgtgtaa gccttttttc
     2461 ttttggattt cttttaattt ttccggggtc acgtcattgc cctcaggatc gacgactttc
     2521 attccttcga tatgatgacg cataccggaa cgaaaagctt ccaaatactc tttgcgtaag
     2581 ttgatttgtt cttgttcttc ggcttttgag agaccatttt ccttcttttt acgtgcaagt
     2641 tcattgatcc tctctatttt ttcaggtgac agcatgctca gccctccttt tactctcctt
     2701 catattatat aaaaactgaa aaaaaaacaa ctagataaac taaataaaat acgaacattc
     2761 gtttgattta gcgcttttta tatggtacta tttatttatc aagtgaaaga aggtgtacct
     2821 tgtggcccga caaactgaat caagacaaat cgaagtctta cgatatatcc atgaacaagt
     2881 aacccaaaaa ggatacccgc cgactgtccg tgaaatcggg gaagctgttc agctttcttc
     2941 tacttctact gttcacggtc atcttgcacg gcttgaaaaa aaagggttta tccaaagaga
     3001 tccgacgaaa cctcgtgcaa tcgagttgac aaaacaaggt ttagaaaaga ttgggattcg
     3061 tccaacaact attccagtac ttggcgtcgt aacagctgga gaacccattt tagcagtaga
     3121 ggatgcttca gacttctttc caatccctcc taatctttct tccgaagaag gcagtctctt
     3181 tatgctgaca attcgtggag atagtatgat caatgctggg atcttggatg gtgacaacgt
     3241 gatcgttcgg aagcaagaaa ctgctcaaaa tggcgatatt gtcattgcaa tgactgccga
     3301 ggacgaagcg acttgcaaac gattctataa agaaaaaaac tttttccgtc tgcagcctga
     3361 aaacgacttg ctcgaaccaa tcatcttaga tcaagtatct atattaggac gagtagtagg
     3421 actttatcgt agtcatatct attaattgct actaataaaa gaaaactttt taaaaagagt
     3481 ttgagacgat ggtctcaaac tcttttttcg cccgggttct tcttctttca aatttttatc
     3541 acttccctct ttacaaaccg aacaaatatt cgtatactaa atatacgaac aattgttcgc
     3601 ataaattaac gaggtgagaa ttcatgaaat caatgagacg tcttggctta gtattaactg
     3661 ttttgttttt aggtattatt attggtaatt tcggcatacg tccagcttta cttgtgtttg
     3721 ttccgttatt tactttgtgg ttcatgttgt gggatgaaaa gaagtaccgt caatctgagc
     3781 ggcgcagaat tcatgaagat catttctatc atgagcccta ctacagatcc aggcaatcat
     3841 aataaggatt gactgctaat tattcaaaag acaagaagtt aaacttttac gggggtgtga
     3901 caaaaatcgg tcacatcctt ttttcgaata aacggtgttc taaagccagt ttttcggtat
     3961 taagtcaaat tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttccat
     4021 ttataaaaaa atagcgaccc tcaaaagtca gatagagaat ctgacttttg agggtcgctt
     4081 taaaatcgct tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca
     4141 ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga
     4201 tcaaaaatgc gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt
     4261 tgattgttcc tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa
     4321 tggcatcctt atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga
     4381 tggtttctcg aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat
     4441 aagcaacagg acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc
     4501 gtttttcttt tgcataattt acgatttgct tcgtagcttc ccgataagcg tcaccatctg
     4561 ccatcagtgg ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg
     4621 caatataatc ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg
     4681 gtaggaatat cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgccgctgg
     4741 tataatctgc tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg
     4801 ttctctattg taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt
     4861 tcagggatac gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct
     4921 tgagaatgga tcagtttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa
     4981 taggcctcat caggcgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa
     5041 acttctttca gatccgctaa atcatctgga caatccataa taactagaga gttcggtttt
     5101 cttttttcaa ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg
     5161 ataatctcag gcacgaattt caacgtagaa gaatcaaatg ctaaataaag gctaccttcc
     5221 gacgttcggc tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc
     5281 acagaaaaat cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa
     5341 agctttccta ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca
     5401 aaagcgaccg catcaagctg actcgatgca ttggaaagga caaatttcag atgctgttgg
     5461 tttgctccga ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa
     5521 ggattatccg taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact
     5581 tcatttggca gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga
     5641 tgatcgacta tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc
     5701 gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca
     5761 agcatttcaa aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct
     5821 tcttttaaag taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca
     5881 atgcccaaaa cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta
     5941 ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg
     6001 atctcattca atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac
     6061 gagacggcag gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa
     6121 aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc
     6181 tgaagtccta tccgttctga atgatgaatg gcattgattc ctaatgaaac aaggattcga
     6241 ttctcatcag tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat
     6301 tctacaggcg gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc
     6361 aaatctggaa atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct
     6421 ggtaaattct caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga
     6481 gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt
     6541 ccttcttcga tcttttcttg gtatacagat tggtttggtc catacccatg ctcaaatcga
     6601 tttggcagga atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt
     6661 gcactggtga tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt
     6721 acagcttctt ggattcggct gattgcttta tccatatcgt gcatcagata cggatcatgt
     6781 aattcttgtt cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt
     6841 tgccacagta acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag
     6901 gattcagaag gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca
     6961 ctcctaactt agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa
     7021 aaacagatca gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt
     7081 atgaaatatc cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt
     7141 tcttccggcg ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa
     7201 gcagcttcca actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct
     7261 tcaattttct tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt
     7321 tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc
     7381 agcgcagaac cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt
     7441 acaggaacat ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat
     7501 ccaagaatca cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg
     7561 ctggctaaat aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa
     7621 ataaacggtt gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc
     7681 aacttggcag gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca
     7741 gccttatcga agaaatctgt ctcgattgga cctggattca ctgtcgtaac agctacgcca
     7801 agtggtttca attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca
     7861 gaataaaccg ttgattttgc agttgccatt tttccagcca tagacgcgat attaatgata
     7921 tgaccttgtc ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catcattcct
     7981 aagatgttga cttcaaacat atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg
     8041 aaaatcccaa aacccgcatt gtttaccaga atatcgacgc gcccgacttc ttccatcacc
     8101 ttttcatata attcgtccac gctttcagga ttgctgacgt ctaccttgaa agcataagcc
     8161 tctttgccgc tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc cattcttcgt
     8221 gcgcaactta taacgattgc tccttgcttt gctgcttcat aacagatttg tgcgcccaga
     8281 ccgcctgaac tacctgttac taaaacaact ttatcttcta aattcatgcc tcttcctcct
     8341 cgcgaaatgg aatctcaatg ctatcaaaat ctttcatgat cgtcgtgttt tcaaacactt
     8401 cttgtgcttc tttttcaagc tctaaagctg cttttcctaa atatctcgca ctgatatgag
     8461 taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg
     8521 aatggaaata gttgtgcgcc attcttgctt catctttatt aaatgtgctt tcatgtacca
     8581 gaatatcgct attttccgca agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa
     8641 tagtcacgat tcgtcctttt ttatctggac ctacgaaatc ttttccattg atcgtccgcc
     8701 catcttctag ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt ccagctggaa
     8761 tacctaattc ttttagacga tccacctgca attctccttt atgatcatgt tcaaccacac
     8821 gataaccaaa actgtcgatc ccatgattta gtttttttgt ataaacagaa aattgctgat
     8881 cagtaaaaat cggggctgtt tcatttagct caatgaattt cagcggataa cttaaacgcg
     8941 tttgcgaaat acctaaagag acttttatgt attcctctat cccttttggc ccgtaaatct
     9001 ctaatggcgt atccccacct tgaaatgacc ggctgctgat gagcccggga agtccaaaaa
     9061 tatgatcgcc gtgtaaatgt gtaataaaaa ttttaccgat ttttctcgga cgaatagtcg
     9121 ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact tcattgcgct
     9181 cgtctaaaag tttcaatgct atacttgtca catttctatg ttttgcagga acacctgcac
     9241 ctgttcctaa aaattgtaat tccataatct acctgacttt ctttaagttc ctctttaatt
     9301 ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa
     9361 cgttctaaag cgatagcgtt ttctttgaaa attactataa aaacagcagc gaacccgttt
     9421 gagatcctct gctgtttttt attaatttga agatccagaa ctttcattgt cgttggaagt
     9481 ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat tttcagcttc
     9541 tttcgcatca tgcacatctt tttcgacatc ttcataatcc aagcgggtaa tttctccgcc
     9601 taattccatc attaccaatt gattcagtgg gcgattgtcc tcttcatcag caatcaagat
     9661 cagtcctgta gctccttcac cgattttatt tcctacaaag tcgaacacgg tctgggcatc
     9721 ttttatttct ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc atcctaataa
     9781 aatccctagc ggaccaccta atacaccaac caacattccg attaatccac ccgtagaaga
     9841 tttgttatta ccggtaaagt cgataaaatc ttctattttg aattgatgtg tcccattagg
     9901 atcatggctg acaactgcca tctgttctcc tttaatcgaa cgaacagcat gtaacttttt
     9961 gatttctgaa aatgcctgat atgcctggct ttctacttga aaatttaaaa tgattactcg
    10021 tttgctcatt aaaaacgccc ctttcatacc ttcttgttat ttattttacc tttaattgta
    10081 aaataagaaa agaaaagcac tctttttttc caataaaata gggctgtgac aaaagtcgta
    10141 tcacagctcc tttttccgaa taaacggcgt tcttgaagct gatcacttct gtcacaacct
    10201 cccttaaatc tgaatctata ttttttggta tttgttttta tttgcttgcc tattcgacaa
    10261 attcaaattc aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa
    10321 gagcttcatc cacacccata ccacgtaatt gacgagcaaa acgcatcact gtttcatcat
    10381 gatcgaaatt cgtcatttgg aataattttt cgatcttctc accagataag atccacgttg
    10441 catctgaatc acgatcgatt tggaactcag gaccttctgg attgaatcca tagtgtacag
    10501 tttcttcttc cagttcttct tcgtacaatg ggaattctgg cgttacttct aacaaatctg
    10561 ctgtagcatt caataacgcg tccaatcctt gtcgagtcac tccagaaatt ggaaatactg
    10621 ggatgtcatc agcaaattca tcttcttttt ccttgttcag ctgttctttg aatttcacta
    10681 gattttccgg tgcatccggc atatccattt tgtttgccac gatgatttgc ggacgctcta
    10741 gcaagcgtaa gttataagta gataattctt tgttgatggc aagatagtct tcataaggat
    10801 ctcgtccttc catcccactc atatcgatca catgtaaaat tactcgtgtt ctttcgatat
    10861 gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc aagcctggca
    10921 agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac cattcctaga ttagggacga
    10981 gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga tgaaataacg gatagtaaag
    11041 tcgatttccc gacagatgga aaacctacaa gtccaacgtc tgccaatact ttcaattcta
    11101 gttcgatttt tcgctcttgt cctggttcgc cgttttcagc aatttccggt gcaggattcc
    11161 gaggagaggc aaaacggata ttccctcgtc caccgcgtcc accttttgcc acaactagtg
    11221 tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct cggacagtgg
    11281 ttccttgtgg tactttaaca taggtatctt ctgaaccacg accgtgcatc cctttactca
    11341 tcccattttc acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac
    11401 gtaacccttc atcgacgatc aagatgacat cgccgccacg accaccgtct ccgccagccg
    11461 gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc
    11521 cagctttcac atcgatcgtt acttgatcta aaaacatgga cataattaag tcctcctatt
    11581 attcatttat ttatttgact gcctgaattt ccacaataaa caaaagtctt acttatttta
    11641 tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga ttgtttacct tcttcttttt
    11701 ttatcatttg gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa
    11761 tattctttgc gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac
    11821 agcaccccta ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt
    11881 gggcgtattg gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta
    11941 gtagcaatca acgacttaac aagtccaact atgttagcac aattattgca atttgactct
    12001 actcatggta cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc
    12061 gaagcaactc gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac
    12121 ggtgtagata tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg
    12181 catttagatg ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc
    12241 atcgtctata acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt
    12301 tcatgtacaa caaactgttt agcacctatg gcttatttct taaataatga atttgggatc
    12361 gaagttggta caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga
    12421 cctgtaaaag gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat
    12481 tctactggtg ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa
    12541 ggacacgcac aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg
    12601 aaaacaaaag taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat
    12661 ccttcattcg ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa
    12721 ggttcgatct ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt
    12781 aaaactgttg catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta
    12841 gaaaaattcg ctaacttgta atttgataga tcaatcgata aaagaagcct ttgactggat
    12901 ccagtcaaag gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg
    12961 gcttaaaaat tgttggacta cttgctgata tttttcagga tctgtcttgt atgcttccgc
    13021 atgctcggct ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt
    13081 atctaacatt tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg
    13141 ctggtttttc ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac
    13201 ttttgtcact agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc
    13261 tagctgataa gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt
    13321 cacattatct ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac
    13381 accgtataga acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg
    13441 tacatagtct ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg
    13501 tcctcttgca tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt
    13561 ggtcatcgtt tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt
    13621 cgtttcagca ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca
    13681 atcgcttcgg ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg
    13741 tgttgtacca ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt
    13801 ataaaaataa ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag
    13861 gattttcatc caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg
    13921 aattagtata atcaaagaat ctctactatg caatgttcat ttttataac
//
LOCUS       DACCXO010000069        13719 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.070, whole genome shotgun sequence.
ACCESSION   DACCXO010000069 DACCXO010000000
VERSION     DACCXO010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.070"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            51..245
                     /locus_tag="IVY70_001861"
     CDS             51..245
                     /locus_tag="IVY70_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958986.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            514..813
                     /locus_tag="IVY70_001862"
     CDS             514..813
                     /locus_tag="IVY70_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7958987.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(910..1344)
                     /locus_tag="IVY70_001863"
     CDS             complement(910..1344)
                     /locus_tag="IVY70_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7958988.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1576..2286)
                     /locus_tag="IVY70_001864"
     CDS             complement(1576..2286)
                     /locus_tag="IVY70_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP7958989.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2276..3136)
                     /locus_tag="IVY70_001865"
     CDS             complement(2276..3136)
                     /locus_tag="IVY70_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP7958990.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3141..3779)
                     /locus_tag="IVY70_001866"
     CDS             complement(3141..3779)
                     /locus_tag="IVY70_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP7958991.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3794..4093)
                     /locus_tag="IVY70_001867"
     CDS             complement(3794..4093)
                     /locus_tag="IVY70_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7958992.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4136..5620)
                     /locus_tag="IVY70_001868"
     CDS             complement(4136..5620)
                     /locus_tag="IVY70_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP7958993.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5640..6101)
                     /locus_tag="IVY70_001869"
     CDS             complement(5640..6101)
                     /locus_tag="IVY70_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7958994.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6119..7186)
                     /gene="ulaG"
                     /locus_tag="IVY70_001870"
     CDS             complement(6119..7186)
                     /gene="ulaG"
                     /locus_tag="IVY70_001870"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP7958995.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7404..8174
                     /locus_tag="IVY70_001871"
     CDS             7404..8174
                     /locus_tag="IVY70_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7958996.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8447..9274)
                     /locus_tag="IVY70_001872"
     CDS             complement(8447..9274)
                     /locus_tag="IVY70_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7958997.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9842..11077
                     /locus_tag="IVY70_001873"
     CDS             9842..11077
                     /locus_tag="IVY70_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP7958998.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPFWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12076..12336)
                     /locus_tag="IVY70_001874"
     CDS             complement(12076..12336)
                     /locus_tag="IVY70_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP7958999.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12363..13664)
                     /locus_tag="IVY70_001875"
     CDS             complement(12363..13664)
                     /locus_tag="IVY70_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7959000.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4405 a         2817 c         2169 g         4328 t
ORIGIN      
        1 ttacatatat ttgaatatta actaaaaaca gcaagaaaga agaggatcaa atgtattatg
       61 tagaagtaaa aacaaaaggc gtcaaaaaca aacaatatgt gaaaggtatc agtaacgaat
      121 atcctttact tggttcatgg aaagaagctg ctcctttttc caaaccatgt gccataaaaa
      181 taaagaacga gctagaaaaa gaactgactt gtggtaaagc tgttgtagat atcatcgaaa
      241 agtaaatcca atgtatagaa aggaagatca cacatgaaaa gcgacgtaca aattgctaag
      301 attatagaat tagaagaaga agacgcagtg ttgacaattg aaaaaaaata aatgtttgta
      361 taccatatga ttatttcaat atgtatcctg gaattggaga tctcgtagag ctatatcaag
      421 atgaatcaaa tatacttgtc attcatcatg aaatatagag acttataaaa ttgtttattt
      481 ttataatgta tcagtataga aaggaaataa aacatggatt atactgttga atttgacagt
      541 gaactactta agcaatttcc acaagaagaa catttcatgt ataaagaagc aatttcccac
      601 aaagtgacag agtatttgga gcataatttt tttcgtataa agccagtgcg tactcgttcg
      661 gcaaaaggaa tatacgagat gaaagtcaaa attaataaaa aaaattatcg catagcattt
      721 gctttaaaaa aaaatcatgt gttcattttt tatattagtt cgaatttgca aaaaattgta
      781 tttgataaag aggtgagtaa aaaaataaaa tagctgagca ttggaggata gattgagtga
      841 tactaagtct atcctcttgt ttttctaaag aactaagcta gaattttttc ttttgtacgt
      901 tattatatgt tatttgtcat taccaaaaag cacctttttt cttctagtat ctatgacata
      961 agaaatacca cccatttcct ttacttgaac aaatcctact gattcataaa atttttttgt
     1021 tttctcagta tcatctgtca ataattcgat ttggactggt ccataactag tgatcttctc
     1081 caaagcttca ttcaaaagca ctttgcctat tccttggcgc tgatacctcg gatgaatgag
     1141 gatatcttga ataaatgcga tcgtcgcaaa atcagtgatc caacgaacga ttccgataat
     1201 cttcccttcc ccattacgct ttatcagaga ttcagattgt tcaaatgctc tagctaacct
     1261 tgcagtgtct tttgtgtaat tcgtccaacc aacgctctta tataattcag aaatctcatc
     1321 gatcataagg gaatcaatat tcattttttt cttcctttct tatacaattc tccctctaat
     1381 aatacagttc tttgactatt taatctatca catataactc tcctgtaaag attctctcga
     1441 ttccttaaaa ttcctctttc cttttagaat gcatactttt tgataaataa aaaggttgtg
     1501 ataaattttc ttcacaacct ctgtttctct ctatcaagtg ttatctttta attttctacc
     1561 cttaccctag catctctatt gcccgtagta tgcatacttt ccatgtttgc gaaaataatg
     1621 tttatctagt aaatattgcg ggatttcaca tatattgggg ttgatcagct cgttttcttt
     1681 ggccatcaga caaatttcat ctaagatcaa actgttttct actgctttca taggtgaatc
     1741 cccccatgtg aacggtccat gtccatagac aagtactcca gggacttcgt ttggatctaa
     1801 ttttctttcc ttaaacgtct cgacaatcac attccctgta tgaacttcgt aagcttctct
     1861 tacttcttct ttcgttaatt ggcgtgtaca aggaacctta ccataaaaag catcagcatg
     1921 agtcgtgcca taagctggca aatcgcgtcc tgcttgcgcc cacatgacgg agtgcgtgga
     1981 atgagtatga gtaatagcag ttatatcttc aaaagtttga tacaaaacaa catgtgtggg
     2041 gagatctgaa gatggtttta agctatcttc ttctattata tttcccgaca aatctgttac
     2101 caccatttga tctgcttgca tgcattcata tctgacaccg cttggcttaa tgacgatgac
     2161 tcctagctca cggtccactt cactcacatt gccccaagtc aatttgacta atccgtatcg
     2221 cggcaatgcc agatttgctt caaggacacg ttgcttcatt gctgcaattt tttcttcaca
     2281 agacatcata gccagcctcc ttcaatttag aacttaagta gtcttttgcc tgctggattt
     2341 ctcttcgaaa gtcttggctt ttttcactcc acatttcaat cagaaatgtt ccatcataat
     2401 ctagacgttt caaatgtctt aataatccaa gaaaatcaac acaaccgcta ccaaaaggaa
     2461 catcacgaaa ctggcctgaa tattcttttg ttacagggta agtatctttt aagtggatag
     2521 cagtgatata atcgatccct ttctcaagtt ctcgagccgg attattttcc ggccatgcgg
     2581 acaaattgcc taggtctgga tatacttgta gaaaaggtga atgaatctgt tctttgattt
     2641 ctaaaaattt tgaaatggag ttcataaatg gatcatccat gatttccacg gaaagaatga
     2701 tgccatattt agaagcttcc tttacgcttt cttttattcc ttcgataaag tcttctctgg
     2761 aattcattga tttctcttca taataaacat cgtatccagc tatttggatt actttgacgc
     2821 ttagattatg cgccaaatgg attgcttttt tcattagttg tttcgctgct tttttctttt
     2881 ctggatcagc agaaccaaac ggaaatcgcc gatgcgcact taaacaaatc gaatggatac
     2941 gtacaccgga gctgaacatc tcttctctca gctcagctat cttttcttca gaccaatcta
     3001 atcgtgctaa ccgctcatcc gtctcatcaa tcgacatctc gataaaatcg aagtccattt
     3061 cttttacaag ggaaaaacgt tctttccatg aaatatcttt gggtaacgct ttttcataaa
     3121 tcccaatttg agccatttcc ttacccccag atacgaatga tctcatcttt gaagttttgt
     3181 gcggccttca tcggatcatt tgctgctgta ataccacgac cagtgataaa ggtatacaca
     3241 tcgatccctt caaataagtg taatgtgtgt ggatttaatc caccagttac agaaacatta
     3301 aagcctagtt caatcagttt tttgatcttc gataaatctt tttcacccca agtttcacca
     3361 gataacagcg catcacggct ttgatggtag attacctgcc ggataccgat gttatgccaa
     3421 tccatggctt gttcaaaagt ccaattgccg tagagttcta cttgcaattc acctatttct
     3481 ttttgtgctg cctccatagt tggaatcgtt gcacaacaaa tgactgtcat ccagtctgct
     3541 cctgcttttg caacattact cgccactgtt cctccagcgt ctgcacattt cgtatcagcg
     3601 actagttttt tatctgggaa catactgcga atacagcgaa tcgctttttg tccttcctgt
     3661 aagcaaagaa ttgtgcccac ttcaataata tctactacat caccaacttt cataacatct
     3721 cctaatgcat gttctaagtc attgtgatct aacgcaattt gcaaattcgg cctgctcatc
     3781 ttagttcctt cctttacttg tttttttggt accactttgt ttcagcaaat ttttcactga
     3841 tttctttgac tgacatcaca tttttgatac cgataactgt aactcctttt ttctctgcct
     3901 gagtaaacat attcaaaaaa ttgactggcg taaaaactac atcgtattgg ccagctgaac
     3961 tttttccctc tgaaatggca caatgatgga ttttggtaat tggtacgttt agttctttca
     4021 ttgctttttc cacacttctc atcatcatca agcttgttcc tgatccattt gcacacgata
     4081 ctaaaattct catcttaatc cctccattat gagtgttgtc taatcagatt ttcttttata
     4141 ctctctgctt ttctgcatat gtgtcatagt cttctacaat caaaaaataa ccatcaggat
     4201 ttttccgata ttgtaactgc ggaatagcca ataaaccaat gacaactacg gcaattccag
     4261 cataacctag gactttcatg attactgtaa acaatggcca agctgttgcc cagtcgaaca
     4321 tcccgatata accaccgtat gttgacatcc caatccatgt agcgaataat gctgaaccgc
     4381 ctacttgtat gattcctgaa agaaacggga acaggcaagc ggctttgaat ccacctcggt
     4441 tattggcata aactgctata acggcattat caaagaacaa cggaatgaaa ccggctatga
     4501 cgatcgttgg tgatttcaat aaaatcagta aaccaatcat caaaaactgt cccaaagcac
     4561 caaacaagaa cccaatcgtt accgcatttg gcgaaccaaa gccaaaagta gcagctacgt
     4621 caattcctgg aacagctcct ggtaaaatcg tgtttgagat tccttggaaa gattgagtta
     4681 gttcatcgac gaatgtccga acacctaatt gcaagatcgc cagatatacc gcaaaattta
     4741 aagaagtcgt cataatgtag aataagaaac tctgcccttc ttgcatgaat tgcgcttgaa
     4801 tcaaataatc tttgcctagt acaatcagaa tgatcccaaa gaaaaatagc atgagtaacg
     4861 aagtagcaac catattctca ttgaaaatcg acaagaatcc tggtaatttg atatcttcaa
     4921 tttttttgtc ttgcttgatt gatctatttt tcttttcatc ccgctttttc atccactcag
     4981 ctaattttgc aaatataaag attccgaaca tttgctgatg tgccactgca aatccggcac
     5041 cttctgtcaa ttcctgcgtg attcctactg ttaagttaga agctactgtc caataacaac
     5101 ctaaaatcag tcccataaaa atcaatgctt gtatcctacc tagatcagga aagcaaaaca
     5161 gtaaaatcca aaaagcggta gctgcttgtt gaacctgaac gtttccggta gtgaaaacgg
     5221 aacgcagttt cgtatatttc tgaaaacgga ctaataagat atttaaaaca aatgcaatca
     5281 gtaataaaat catcacatct ccaaaggtac ggccaaatgt ctccattaat ccagcatcca
     5341 cagcattttg tccaaaataa ggatctgtaa ccatggcatc caaattgaac cgttccttta
     5401 gtccaaccaa gattggccga aaattattga ccaaaccacc agagccaaca gtcaaaataa
     5461 agtaaccgac cgttgccttt aagaatccag cgatgcattc atgaagcggg cgtttcagca
     5521 aaatatagcc taataacacg ataaaaccaa tcaggtatgc tggctgcgtc aagatatttg
     5581 ttgcaaaata cgtccaaatg cttaacaaaa attcccccat cttttttctc ctctatcaat
     5641 taattatatt ctgccatcac aacttggtaa tcatcgatgg agttcgtaga gatgagacgc
     5701 tcaaccaatc catctgtcat taataattcg gataattttt ggatattttc tagatgttct
     5761 tctggatctt ttgctgcgag tgtaaaaaac aaactagccg ttttctcctc attctcccct
     5821 tcactttcaa aacggattgg ctgtttcaac ttggtaaatg aaatagctgt tccaaaaaca
     5881 ccatcacttt tttctgaaga atgcggcatt gctacttgag gtgcgatcac aatataagga
     5941 ccgtattttt ctacacaatg gatgatctcc tccacatacg tttcatcaat aatttcctta
     6001 tcgatcaata caagacagct ttcggcgata gcttcacgcc aatccgtgat ttctttatct
     6061 gggtaatgaa tcaattgatg gtcaaaaaag tatttcagca agaaactccc cttctttctt
     6121 ataaaaacga cggaaacggt gtatcatttt caatcgagaa gacatcttca aatcctcgat
     6181 aaaaattaaa ctcaagatca tctttgtttg tcggatagat gaatttcccg cctacttgcc
     6241 agatataagg tttaaatcca tacttcaggc gatccttttt gaatttccaa atttcagtga
     6301 tctcttttgg atcagccata aaattagacc aaatatcgta gtggacagga atcactactt
     6361 ttgcattcaa tgactccgcc atgcgcagca tatcaacaga agtaactttg tctgttatcc
     6421 ctcgtgggtt ttcaccatag gcccctaaac agacatcgat tttgtgttca ttaccatgtt
     6481 tggcaaataa attggagtaa tgtgaatctc ctgcatgata aagattccct cctgatgttt
     6541 cgaataaata gtttactgca atttgatcca tctcctgagg catttttcct tttaatacct
     6601 cttctggatc ttcacaagtg accaacgctg tccgatcaaa cgcttctaaa gctactacat
     6661 tgatgtcttt gattttgact gcatcacctg gctttactgt aatcgttttt tcttcaggaa
     6721 ttccccattt ttgccacgtg cgaaccactt cttttggccc aatgaatgtt gcttcaggac
     6781 aattttgatg aactgctgcg gctgtatgga tatctagatg atcggaatgg atatgtgtaa
     6841 cgattaaagc gtctacattt tttatcgcaa aaggatcgat tacgaaaggc tgagtacgta
     6901 aattaggctg catattttga acgccactca tacgcatcat ctgatggcct tttttcatca
     6961 tgccattccc atgactacgt tttcccgtcc cacaccatag atcacataaa atattcgttc
     7021 cttcatgact tttcagccaa attcccgtac agcccagcca ccacatagca aaagttccag
     7081 gatccactac ttctgtttcg atttcttcat tcaagtatgt gccccactca ggaaatgttt
     7141 ctactaacca cttttctttt gttacttcat tgacagtttt actcatttgc ttgctccccc
     7201 catatcgttg ttccaactta caagtaaata ttaatgtgta tcagcatcca tgaaaaccct
     7261 tttatatgac cgtttgtttt ttatatgatt ttctttcagt aatcaatgat tgtttcagta
     7321 taataaagac agatatgaat gtttaaatga acaactagtc aaataaatcc atggatcaag
     7381 atgtccctca ggaggcaaaa gtcatgaaaa actcacttgt caatattgaa aaacgtcaaa
     7441 aagaaatttt ggaactgctt cagcagactg aacaactatc aactttcgaa ttagctgaat
     7501 cccttgatgt atctattagt acgatacgaa gagatttaaa tttattagaa gaaaaaaacg
     7561 agatcatacg caattacggt tattgttctt ataattacaa aaaccagact gattttgatc
     7621 aatctggtcc tgaaaggatt aaacaagcga ttgccagaga agctagtaaa tacattaacg
     7681 attatgatac gctgtttatc aattctagct cgactgcgtt gaaaacactg gattttcttc
     7741 aatctaacca tttgacagtc attacaaaca acgtgaaaat caattactct ccgcacaaag
     7801 ataattgtag ttatatttta actggtggag agatgcgctt tccaaaagaa gcactagtgg
     7861 gcgacatcgc attaaatacg atttcttcag tcaatgcaga catttgtatc attggctgta
     7921 gcggagtcga tgtagtacat ggggtcacaa caaagatatt aaatgaatca acgatcaacg
     7981 aaatgatggt caacaagaca atcaaatgta agattttagt cgctgatcat cgaaaaatcg
     8041 gactgacatc aaagttcaag attgctgata tttcagtctt tgattatttg atcactgata
     8101 aattttgttc tgaaaaagta ttagaggaga tcagaaatac ggggataaaa accattcaag
     8161 tagattcatt ttaagaacca ggacgtaaat atgtaaatag gattatcaaa aaaatgaata
     8221 acaaatttat cgataaatga aaaacacgct acgctcaaat tgataaagga atcccaagat
     8281 gtatccatcc aatcgatacc atcataatct attgcttctt ttataaccgt tatatgttgc
     8341 ctaaatagga atcaaacaat atcgaaaaaa ggaggtatga caaaagtcac acctcctttt
     8401 ttcgaataaa cggtattcaa atcagaacac tgatttcaca accactttat ttcaggtcgt
     8461 tttcaatcac ctttaccgcg taatcatttc cgttgaaacg cgctattttt tttgcgtcca
     8521 tatatctttc taaataatca tcttctccta attgcttatg gcaagaagca atttcataaa
     8581 gcaagtattc caatgcataa gtcgatttat gtttcttatt atactcaata cccttctcac
     8641 ataattggat tgctttatca taatcctcga ttttacggta ataggtggcg atatcataat
     8701 atacaatgca gaccatcaga ttatcaaaat cagtctcact caatatttta agtgtacgct
     8761 ccacataaac tttcgcaaaa tctaattctc cttttgacaa gtagatcatt gccacgtttg
     8821 cagctgcttg taagtgatgg atatctgttt cggtcgtttg ttccaacact tgattaaaat
     8881 aaaataatgc tgcatctggt tttttcgcat aaagatgatc aagcagtcct tgataaaata
     8941 aaatttgatt taaaagatca gaattactta ctttttcctg atcgatcgtt tgtaaaattt
     9001 tataagattc ttggtatttt gatacagaat tcaatgattg aatctcattt atttttttcc
     9061 tgattttgtc ttcttctgtt tctactaata tatcggagat ctcgacattc agctttttgc
     9121 aaagcgcact taagatattg atgcctggcg acatattctt gttttcgata ttgctgacag
     9181 ttgcttgttc acaaatgcct tctgctaatt gtacttgtgt taaccccttt ttctttcggt
     9241 attcttttag taatgctcca tctactttaa tcaaaacaat ttccttccct ttaaaaaatt
     9301 atttctcctt tatatcatac acaaaaaaaa gttctaaaca accaaaaatg tttagaacag
     9361 aataaaaatt ttaattttaa aataatattc aaattaaaca actggcgtat ctgctccttg
     9421 atttcttttt gtgttatctg aataattatc gtttacagtt ataacacata gtgaaaattg
     9481 tcatggcgag aaagtctaag aatattttta aacataacta tttctttctt acgtgtgtat
     9541 aatgacgaca gcacacctat caattaatat aattatttta tttttttatg ttctcatgtt
     9601 tatttttaat gacacaactt ctcgtattta tctggcacaa catataaatc gatcaaaaaa
     9661 ttgttgcgtg tttttttgaa aattggagtt tcaacttgat tacaaaattt aaatttagaa
     9721 gttgtgaaaa cagcgagaga cagtttaata ttcaaaatat acataggcta ctcgaacgct
     9781 gtgtttttta gtatactagt aagaaacgaa gggatagact cattccaata aggaggattt
     9841 catgaagaca attctttttg ctcctgcagt attcaattta gctgagacaa cacgaatgat
     9901 tgaggttgcc aaagctatga aagaccgtta ttactgtgta ttctttggct tttctaaaac
     9961 ttttgctcct ttgatcacag aaagtggctt tgagtatcat ttactagcgc cgatccttac
    10021 gaaaaaacaa gaagaacaaa tcataaaatt tgaccagttg cgtacactta aaaacccttt
    10081 taccaaagaa atggttgcct ctcgtatcac tagtgaacag agtctgattg ctaagtttga
    10141 gcctcgactg attgtgacag gttcgaatgt gacaattttt ttatcggccc gcaataaaaa
    10201 gattccttta gtatatgtta aaccctatgc tttgagtgaa cctttttttc aatcagagca
    10261 tgcgctgatg ccccacgagt tacgtttctt cggcccttta agcaatccct tttggcactg
    10321 gacaaaaaag cgtatagtaa atacccgatg gatccccaat gccttcaaac acgctgcaca
    10381 aaaagaaatg cttcctttac caaaaacaag tttacaaatg atggatgctg atctaaatct
    10441 catcacaacg cctaaactat ttttggaaga agaacagctg ccggaaaatt atcgaatcgt
    10501 tggtccaatt tttgctaagc tcagtacttc cttgcctgaa gaagtgctaa cttttataca
    10561 gcgaaagcaa caagaaagaa aacgtattat ctatttcgca atgggtagtt caggcaatcc
    10621 gcgtatgtta cgcaaaattt tgttcttctt acaaaaacaa ccggatctag cggttattgc
    10681 acctattgac tatctgcttg atgacattca atctgactca gaagatcttt taataacaga
    10741 atatctgtct agtcatcttt tgcatgaatt cattgatttt tcttttatcc acggcgggga
    10801 agggaccgtc caaactgcct gtgaatccgg caaaccgttt atcggtgtgg ggatgcaatt
    10861 tgagcaaagc tgtaatattg actattgtgt aaaatacgga aacgcaatcg ccctcacaaa
    10921 accagtgact gaaagaaaac tgcagattgc tctgcgcgaa gtttcttcac ccaagattcg
    10981 tcagcaggca cgcacgctgc aagccgtttt tccaataaat ggtccacaac aagcaattca
    11041 agaaatcgaa accttcctga gcaaccaccc aatatagttt caagtagaaa aagctttttt
    11101 aatagagact catcagtaat ttcgctattc gagacccaga cagtgaaaat acaaaaaaag
    11161 taaatagtgg attgaaaagt tagagttact taaattacgg tgtctaaaaa acacaaaatc
    11221 atccttaaaa taaaagatct gacgctatcc aaatgagcat aggtagcgtc tgccatttat
    11281 tcttagtcaa attctttgac acctttctct ggttggatca tctcaacgtt tcttcaaaag
    11341 aaagaccata ataattaggc aaatacattt tcttaaaatg tgtcatatag atctagaagc
    11401 atcgctttgt agagatttgt gccgctagtc ccctcaacaa ttactaaatc aaatgtagga
    11461 tttaccactt tatctttgat aatcaacctc cctcttctgt tgagacaatg atctcgcaat
    11521 ccgatgcata ctcctatata aactgccagc aatccccaca tattgagtag atcatgtcag
    11581 aatgttctat gtcgtcagca acgatacact aaaagtcacg ttcaccatga ctgattgcta
    11641 ttcccagcac aattatttct atacacgttt catgatttga ataaacactc acatcataca
    11701 tattaccatt catacaccat actgcagccc ctactatatg gttgtaagct cccaaatcta
    11761 tatttgactt gatcactttg gtagtagatc tactaattct aaatccattg ctgataaaat
    11821 ttccattttt tcctccttct ttctgccaaa acagttaaaa caataataat ctttatgtaa
    11881 aaaagggctg tgacaaaagc cttgtcacag tcccatattc cgaataaacg gtggtcaaaa
    11941 gcttgcatct gcggtaataa gttcaaccaa acgaaaaata agccaacatt tcacaaaaat
    12001 ttaagaagca attttcgtaa atttcttctt atttcttgaa gctaaccact tctgttacaa
    12061 ccacttttag aacatttaaa gcttcgatgt gacaaaccat ctatgctgct cgatcataaa
    12121 attgatagca aaaattccac aaagtacata tgtcccccat gaaaacaaaa cattcatatg
    12181 cggtacaatg atctttggaa gaatcacgat caaaaatgat gccagtgccc aaatccatcg
    12241 attgatttga atatgatatg tagctagcat attcaatata aggtgactag ccgttaataa
    12301 aaagataaaa acaaaaaata aaatcagcca atacattgtt tcccaccttc taaaaaccaa
    12361 acttagacag tctggacttc gttttctcca cgactttgtt catctgacag catcttttta
    12421 tcataagctt ttacaaatgg ataatagata ataaatgcta tcaatgtgtt gatcatgatc
    12481 aagataacaa cacgccagtc atttcctgct gaaagataac cgccgattgg agctggtaat
    12541 gtccatgttg gcaaaacagt aaatctattg accatttcta atttgactgc aaaataactg
    12601 ataatagtca aaactaaagg aactaagttg aacgggatga acatgtaagg attcaacatc
    12661 ataggagctc caaaaattat gggctcattg atattgaaga taccaggaat aatagaaaaa
    12721 cgaccaacct ctttcaagaa ttttgattta cagaagaata taaacaaaac agataccacg
    12781 atcgttgctc ctgcaccacc aattgttacc gtccattgat aaaactgttc aggagcgata
    12841 tagggtaatt gggtggcagc tgtaccatct gccaatgcct ttgcattctc atccaacatg
    12901 atcagccaca ttggacgaac aacagcccct acaatactca tgccatggat accaaatgac
    12961 cacaacaaat ggatcaaaaa cattggtagt aaaacaccaa gcaaattatt ccctaaaata
    13021 ttggttaaag gctcgaataa cgataataag gcagcgttta catcaaagcc cgcgacgctt
    13081 cgaatcagcc aagtaattgt taaaatgact gcacctggaa tcaatgcctc aaaactacgg
    13141 gcaacagcag gtggcacttg ttctggcatt ttgatagtaa tatttcgatt tttaaagaaa
    13201 cgatatactt ccactgcaaa aatcatagat aagatccccg caaacatccc agatgctccc
    13261 aaagaactca taggtaagac ccagccaaga ggtgcctctt caggagttaa aacgtttaca
    13321 ggaacttgta acataaagaa cgttcctaaa gataatatac cacctgtcac actatctaat
    13381 ccatatgatt tagctagatc cgaacccata acaaaagttg cataaatagc catcaatccc
    13441 atcgtcatcc taaagggtaa aacaatcgtt gccgaataag gggcaataaa ttcattccat
    13501 ccaggaaacg gtaaattgat aaagaccata aaaaatgtcc caatcaaaat caatgacaat
    13561 gttgaaacaa ccccattacg aattgctaat aaatgacgct gtgtaccaat ttttgccatt
    13621 ggtgggacaa gtttctcttc gacccatttc atcagctttt ccactaaaac cactcctttt
    13681 caataaaata tgaaccattc attgcatgac aacgcaaag
//
LOCUS       DACCXO010000070        13694 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.071, whole genome shotgun sequence.
ACCESSION   DACCXO010000070 DACCXO010000000
VERSION     DACCXO010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.071"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            161..475
                     /locus_tag="IVY70_001876"
                     /pseudo
     CDS             161..475
                     /locus_tag="IVY70_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(780..1340)
                     /locus_tag="IVY70_001877"
     CDS             complement(780..1340)
                     /locus_tag="IVY70_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7959001.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            1482..2096
                     /locus_tag="IVY70_001878"
     CDS             1482..2096
                     /locus_tag="IVY70_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7959002.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            2285..4165
                     /locus_tag="IVY70_001879"
     CDS             2285..4165
                     /locus_tag="IVY70_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP7959003.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            4244..4471
                     /locus_tag="IVY70_001880"
     CDS             4244..4471
                     /locus_tag="IVY70_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP7959004.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            4476..5018
                     /locus_tag="IVY70_001881"
     CDS             4476..5018
                     /locus_tag="IVY70_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7959005.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            5245..5961
                     /locus_tag="IVY70_001882"
     CDS             5245..5961
                     /locus_tag="IVY70_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP7959006.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(6021..8039)
                     /locus_tag="IVY70_001883"
     CDS             complement(6021..8039)
                     /locus_tag="IVY70_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP7959007.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8052..9050)
                     /locus_tag="IVY70_001884"
     CDS             complement(8052..9050)
                     /locus_tag="IVY70_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959008.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            9248..10489
                     /gene="sstT"
                     /locus_tag="IVY70_001885"
     CDS             9248..10489
                     /gene="sstT"
                     /locus_tag="IVY70_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP7959009.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            10636..11271
                     /locus_tag="IVY70_001886"
     CDS             10636..11271
                     /locus_tag="IVY70_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP7959010.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            11462..12202
                     /gene="truA"
                     /locus_tag="IVY70_001887"
     CDS             11462..12202
                     /gene="truA"
                     /locus_tag="IVY70_001887"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP7959011.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(12347..>13694)
                     /locus_tag="IVY70_001888"
     CDS             complement(12347..>13694)
                     /locus_tag="IVY70_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959012.1"
                     /translation="YYFFITFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDI
                     FHRASLGEAVALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFI
                     GGTLFLSFYFWDQKIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSG
                     MAPSDMLTHILNNNYNNYTIGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTN
                     LFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLAL
                     ILHLSGVSNSIHANKDSLTLIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLG
                     NQEINPTTHFMSNKLTIELDNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTT
                     ELTIPPGINKVVITYQYTKLAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         4591 a         2160 c         2677 g         4266 t
ORIGIN      
        1 ttctttatag agaattgggc tatttttaat ggcaaagagg ggaagaagat gtttcaaaac
       61 aaaggacaac gctatgttgt aaaaggagta aaggaatctt actggaatca aaaatcttta
      121 ttgagaaaat tatctttgaa tttaagcaca aagaaatgaa ggtacactag atagcaatga
      181 agatatcttt gtcattaaac gtcgctattt tacacaagta agtgatgatt tgattcatgt
      241 cttttccaat gagtggtttg tttctgagaa aaaattacat gcttctaatc tgcaatatat
      301 gccaggtgaa gatccaattc ctaatatgaa agcaattatc aacagtaagg attacgaagg
      361 atataaagcc aaacatccag aagcgaaacc atttaaatac ccacaagaaa tgaagcgagc
      421 atggcgtaaa atgttagatg atgagctgat tccccttgaa aatgaattga ggtaaacgag
      481 aagaattcaa taaactggat gataaaaata taatatgact ataaattggt tacagcttca
      541 acctataagt tgtaataaat tagcctaaaa gtaatttatt aatgatgagg tgaatttgat
      601 tggtacaaca agatatatca cttagagagg gagaagcagc actacaaact acttttcaat
      661 aagtaggaat tatagaaatt ttactcaaaa aatcttcagt aaacaatttg gctaaataag
      721 attcagttgt tatacgagaa ggttacggtt cttcttaact aagcttagta tgtttttatt
      781 caaggcatga ttttctctat gtgatcattc agtttctgta agagttctaa taattctttt
      841 ttggagtact tgtaattgct ttctacccac cgattgagaa cggatagaca accttggacg
      901 tggtacgtag taaagaatct catttcttct gtgataatcg ttttcttgtc ttcatatagc
      961 catatgttca gatagttttc ttcaataact tgtttaactt tcttctgaaa cgaaatatca
     1021 ccatggttga ggataaataa gcgatataaa aaagtatttg cgtaaacata gtcaatcaag
     1081 cgagtgtata gttcctcata gctatgggct ggatcataag agagaatttc atttagatcc
     1141 cgaaaaaagt tgctttctaa ttgatcatat agatcataga tatccttata atgtgtataa
     1201 aaagtcgaac gatgtacatc tgccgtttct gttagttcgc gtatagtgat tgcttgaatc
     1261 tttttattta ctaataatgt ggatagtgtt tcagccaaag ctttttctgt ttttttgacg
     1321 cgtctatctg tctttttcat ttacctatct ccttatccga catatcaaag caaagctgtc
     1381 gcttaatcga caaacttata gatgattgat tatagatagt agacactgtg tctattataa
     1441 tgtaagtata acacaatcat tgaagttgag gaggcgaaat gatgaaacta acttttaaac
     1501 ccttagaaaa gaaagattac aaaaaagcta tctattttgc tgttgaagga atgaactttg
     1561 agagatatat ggaaaatgaa cttctgctga atctttacgg aagatatttt ttgtatatgg
     1621 agttgaatcg agcaactcaa attcttgctt tgtatgcaga ggacgagttg gcaggtgttt
     1681 tgctggcgga gataaaagga gaaaaaaaga aacatcgttc ttttggacag caaatatatg
     1741 ttaagatatt cgatttttta caaaatatat ttgctaaaga aagtaaagga gcttacgaga
     1801 cagcaaatga acaaatgctt gcagcatatt taaagaagca ttcgccagat ggagagatca
     1861 tttttttagc tgcaaatcct gatttgaaaa caaaaggcat cggtaccaag ttgctaacag
     1921 aatttgaaaa aagagagaaa ggaaaagaaa tattcctttt tacagatagt ggctgtacgt
     1981 atcaattcta tgaacgacgt ggatttgaaa gactaaagga aaaaattagc aagataaaaa
     2041 tcttagataa agaaatagat ctccgatgta tgctgtacag gaaaatcatt caatgaaaat
     2101 ttcgatagaa acgacaatag ttggcaagat cacttttagt gagtcttctt gctaactatt
     2161 ttttgtagtg tattctacca aaagaaaacc gcctttttga ctaaacttga tagtggtcaa
     2221 ttttttatga tggataagta tctatactta gtctcgtaag aatgaaagga aaagagggag
     2281 aaagatgaaa ttacaaaagt ggatccaaga gcatcgaaat catatcaccg ccttatcagc
     2341 tgtgttgatt atttttggtt ttatcggaaa atttactggt tttgtcttgt tctataacat
     2401 tgctctagcc atagcttcta ttattgcggt catacctatt atgatccatg cgtatcaggc
     2461 agtgaaagtc aaagttatct caattgattt actcgttact attgctgtgc ttggtgcctt
     2521 tatgatcgga gaatacaagg agtcagctat cgttacattc ttgtttttgt ttggtagcta
     2581 tctggaacaa aaaacattga ccaaaacgcg ggagtcaatc aaggctttga ccgagatggc
     2641 cccatctact gctagtgttg tccaagaaga tggaacgact gaagaagtgg aaatcgatga
     2701 cgtggaagaa ggcgatatcg tcttagtaaa aactggtgga gctattcctg tggacggacg
     2761 agtcattgac gggaaagggt atgcggatga agccgcagtg actggtgaat cacgacaagt
     2821 cgtcaaaatc agtggagatg aagtcttctc tggaacgatt ttagcagatg gctacgtgac
     2881 gattgaagca actaaggtcg gagatgatac gacctttgcc aaaattatcg aattagtaga
     2941 agaagcacaa gataccaaat cccatgcgga aaaattcatt gatcgatttt ctcagtatta
     3001 tactccaacc gttttactta ttgcgttggt tgttggtctg attagtcgtg attttcgttt
     3061 agcaatcact gttttggttc ttggttgtcc tggcgcttta gtcattggtg cgcctgtttc
     3121 gaatgtagca ggaatcggta atggagcgaa acggggcatc ttagtcaaag gcggtgaagc
     3181 aatggatact ttttccaaaa tcgatacttt tgtctttgat aaaactggta cattgaccaa
     3241 aggaactact gctgtatcca ctatcaagaa ctatacagag gatgaagggg acagcttagc
     3301 tttagtggct cgattagaaa cgttatccga tcatcctctg gggcgcgccg tgatccatta
     3361 tgctcaggag aaagacattg attttcaaca attagcagtc aaagataatc agacaatcaa
     3421 gggacaagga atcacagctg agattgatgg tcatacagtt tgtgcgggca acaccaaatt
     3481 gattgccgct cacaaacttt ctctcacacc aaagcaagta caagatttac atcaattaca
     3541 acaaacaggc agctcggtca ttattgtagc aatcgacaaa aaaatcactc aaatcatagg
     3601 tgtgtcagat gtgattcgac cagaagtcgc tgaacaatta gctaagttga aacaagcagg
     3661 agcaaaacag ttagtgatgc tgacaggtga taatcagatg actgctgatt atgtagctga
     3721 tatgttaggc attgatgaag tccatgctga attattacca gacgaaaagg ttcaatttgt
     3781 gaaaaaatac caagaacaag gattgcgcgt tgcttttatt ggagatggaa tcaatgattc
     3841 tccctctttg gctgcggctg atatcgggat tgctatggga tcaggaacgg atgttgctat
     3901 cgaaacgagt gacgttgtct tgatgcaatc taattttgaa tcgttggttc atgcctaccg
     3961 cttggctaag aaaactgttt taaatacccg tgaaaatgta gtgattgcca ttggtgtcgt
     4021 cttattttta ctcaacggct tatttgccgg gtttatttac atggctagtg ggatgttcgt
     4081 tcacgaagct agtatcttga tcgttatctt taacgcgatg cgtttattgt attttggaca
     4141 cgaaagcaaa gaagaaaaat cgtaaaactt gataagaatc aattttttta tggaagctgt
     4201 aatctagaat aagactatca agaaaaagga aggaagaact acgatgaaaa aaattattat
     4261 gcaattagat acactagctt gtccaagctg tatgcaaaaa atcgaacatg cggtttctca
     4321 acagcctggg gtaagcaata cgaaagtttt gtttaatgcg agcaaaatca aagcaaactt
     4381 tgatccagat gaagtccacc ctgaacaatt agctgcagtt gtggaaaaat taggttatga
     4441 agtccaatca gcaaaagtaa aagaggcgta aatcaatgaa tatagtggaa gaaaaatttg
     4501 ttcaagaaac aaaacaagca gatatcgacc atcataaacc aactgctggc gccatggtgg
     4561 ggcatgtatt agcaaatttt aaattagaag aaatgaaatt gatccaagca caattgtatg
     4621 ttttgggtac atcttcgcaa gaattaagta cccttttttc agaatatgct gatcaagaag
     4681 cgagatggtt caataaaatc tctcatgagt tagtgattga aaatgaaatc gttccgtcta
     4741 ctttagaaga agtcatgcgt tatggaaaaa tttacgaaca tggtcaaaac aaatatttac
     4801 cagcctcaac aatgttggaa aactttgcaa aagattttga tttccaaaat gtctttatca
     4861 cacgggcaat caaattagct gaaaaagaag aaaaatatgg cttgcaacaa ttgcttgttg
     4921 aacttttaag tttcaataaa gaaatcatct accaagtaca aagactccaa ggaaaaactg
     4981 tccgacaaga tttggatgag gaagacgatg atgactaaaa gtctatgttt cttcaaatac
     5041 aaatataaag cgtgaggtaa attggaaaat cacgaagatt aaaataaaag aagcacccgc
     5101 tgatgtatta cagacaacat cagcgggtgc ttctcagttt ttagaagatt tgaaagattg
     5161 atgagaaaat acgaaaaatc aataaaatca cattgcttat ttgatagaat aaagaagtgg
     5221 gagtaatcga aaggagtttt ttaattgaaa aaaaggattt tagtaacaat tggtctagct
     5281 tttttgttag ctggttgtac caacgggaat gaatcgaaaa aagaatcaac aaaagaaaca
     5341 gcgaaagcaa cagttgcaac tactacaaca actagcacag agagcaagtc aactgcagca
     5401 agtacagaaa cagcagatac ctctacttcg tcagagtctt ccaaaacgac tgaaacaact
     5461 caaacatcta cttcaagttc ttctgtagag gcttcatctt cctcttcaga aagcagcgaa
     5521 caagttgaac aaacactgtg gaatgcagac aaatccgcac aattagaggc atttatgacc
     5581 aaatggggcc aaacaatggg acaacaatac aaaagttata cgaatcaaat gagtgtggac
     5641 ttatatggtc ttaaagtccc tcaagctatt ttgaacgggg agtggaagat ggctattggt
     5701 ggagtgcctg tttctgcaga atggtcagaa agtgggacag gtcaagcaga ctatcaaatc
     5761 acagccgtct attccgatgc agagacagaa ccatatctga aaaagcacgt ctatctattc
     5821 gggtttcagc aaaatcagcc aaaagtattg gtgacacaac aaaatcaggg gaatcccgat
     5881 aattatcttt acttcaacga gacagcgaat aatgaattga aaaatggctt taatcaaatc
     5941 gtttatggac aagcaaatta atgagataaa caaggggttg tgacatgtct tttatcacaa
     6001 ccccttgctc agtttatttg ttagtctttt attttttgtg tcgcccatgt tgcactgtac
     6061 tggttacctg caacaagatc atattgtatc agacgataat cttctagaat ttctttttgt
     6121 ttctcagtta aatttttttc ttggataatt tgactatcct ctgtaacaaa gacctgtttt
     6181 ccattaaaac gattagacaa gctttctgta ggatcaattg ttaatgcagg tagatgatta
     6241 gatacttctg tcaacaacgc atagtaagga gttactttga tattcgcatg ctcaagagcc
     6301 atagcaccaa agttattagg agcaacaact tctttattta acgtagctgc ttgttcacga
     6361 ctatacttgt tgttataaat gaaataatct gtctggtgct gttctaatcc atattttttc
     6421 gaatcattcc cactatagat tgcgggtaaa tggtcaccat aaaaaacaaa ggtaatcggt
     6481 ttatcgattt tgtcgatttc ttggatgaat ttttttactg ctgtatcggt atagtgaagg
     6541 ccttgcatat aagtttgtaa ttcttttctt ctattaggaa tgacagcagt tccttcagca
     6601 gtgtagttca attcactata gtaatcatca taaggcatat ggttttgcat agtggataat
     6661 tggatgaatt gtgacgtttc cgtattgctt tgtaaagctt tcaaggtttc tttgtatgct
     6721 gaatcatccg atatgcgtgg gctttgttct attttatccg tataagttaa tttatccgga
     6781 ctgtctaaat aatagaacct gtcaaaacca aatttatcaa acacttgttt tctgcgataa
     6841 agagcagctg taaaaggatg gatggctatt ttttcatcga atagatcggt aatatttgga
     6901 cttattttct gacgctccac tagctgggta taaggtgtaa ctaacgaagc agacaggtta
     6961 ctgatactca agcctgtcag tccttcccat tccatgttag ctgttccgcc accataaccg
     7021 acggataaca tcaatcctcc tgttccttct gttataatgc ttttgatgta aggaataggg
     7081 tctgtaggta ctgtcagatt agggacacgt gcaggatcac tgaagctttc acttaaattg
     7141 agaacgatcg tctgattgtt taaccagtca cttcttgttt ggttgatttc atctgccttt
     7201 gtgttgtact ttttcattat tccattaatt ttttctttcg aatagccagc aggttcttcc
     7261 atgattttaa tgtcgatatt gtttaggaat tgaatgactg gtccgttatc ttttacggcg
     7321 ccttgctggt taaagaaagt cttgtttact ttaaataaat taaacagcaa gaaagaaggc
     7381 gaatttttgt gattaatgaa aaagacaccc gaaaaacatc ctaataatac agcgatactg
     7441 ataaatcgac gtttccaatg aatcttcaag gaatactgtt tttgtagtct acgttgtaca
     7501 ataatactag taatcaataa taggagaagg atcaatccgc ccacgattaa taagactggg
     7561 ctaaccatcg ataatagttc tgacaatccg gtcagcattt ttaaatctga aggaaagaca
     7621 ggctcttccc gcatttccat tttgattact gtcgaaatag tgagcagtaa gttaatgatc
     7681 aaggtagcag tcaaggcgta ccaaaaacga ttggaaatca acaatagtaa gaagaaaaat
     7741 cccataataa tgatgattgt caggatcatg ggtgcttgtc gctgagtaaa tatttttaag
     7801 gcaacctgga tagtttcttt ccaaccttct tttggttcta aaacgaaaat ctgcacgaag
     7861 gtaaagcagt aaaaaaatgc tgcaactaaa aaataacttt tatgaacaaa aagccaatga
     7921 attctttttt tccaacgctg agttaattgt tcaatcgaat caaaagcgaa gtattcgatg
     7981 aaacgatgaa tgaaacggat tttttgtata taaaataaaa agataactag gagggacact
     8041 gctaataaat atcaaataaa aaactgcccg atatttatca gccatgcctt ccctgagttt
     8101 ggaaattcac gtttcataaa tgtagatacg cgataagaaa ctaaagggaa attacatata
     8161 acttgagtga caatcaaaaa tacagcaaga aaatttcctc ttccttttat acctaattta
     8221 tcagatagca tttctaaaaa taaaaacgaa gagaaagtat aatacattgc gaagagagaa
     8281 tatggtttag aaaacctcag cgctgtatct gtatttcctc tgatgatgag tgaaatttgt
     8341 gtcatcaaaa taatcaaacc gaataaaaca atcaagctga taaatacttg aggaagctta
     8401 tagcggacct tttctttcca gttccattct ttcaaaccat aacccattaa aaataaataa
     8461 aaaatccata aaatactctg tccattctgt aatgcaaacg ggtctttatt aaatagagta
     8521 gagcagccaa ctaatccaat tgttaaaaat aatagtagat atttaattgt tgttttcgat
     8581 aatttattta aaaatccaac tgtataagga atactacaaa aaagcaaagt acaagaaaca
     8641 gcataagtaa agtaattctg actgataggt gttaggataa gccaataatc acgaatattc
     8701 aagctgccag cttgtacgta aaaaaagata ttgcaaacta gtgtggctat taggaatata
     8761 aaggcaaaac gacttaaata atgtaaaact tttaaacggg cgattttttg atattggtaa
     8821 cccatgtaaa gtgggatcaa atttgcacca ataacaacgg aagtttgtaa aaatgtatag
     8881 atgtagaatc gaccagggtt tgttgcatat gtaggaatag tgactgctga aatcaatata
     8941 cgctgtaaaa ccatcattat acaagcgaca aagattgtca ggaagccata actctccttc
     9001 tttatttttt tcaagaataa acacctcaca aaaaaattgg acaaaaacat aagaaaacgt
     9061 ttcctgataa gttgcccatt aataacatcc taacttagaa tatctcaaaa aacaacgtgt
     9121 agacagaaaa agtacaaaat tagcaagaaa tggttgtttg tattgttaga atttttagaa
     9181 aattatacct ttagtttctt tgcataaaat gatacaatga ttgttaaaat tgtgaaaggg
     9241 tggtcatttg ttagagaagt ttttggatat gtcgttagtt caacgtattg ttgttggtat
     9301 tttagtcgga atattgtttg gtatcttttt ccccacatgg acatttattt caatattggg
     9361 aacgttgttt gtcagttgct tgaaggcaat cgcacctatt ctggtttttt tcttgacgat
     9421 ggcgtcaatt gccaagcata aaattgggaa taagacattc gtgaagccta ttttgatctt
     9481 atatttagtg ggaacattgc tttctgcact tgtagcagtt attgtaagtt caatttttac
     9541 tgttcctatt actttacaag aaacagtatc tgaaaaagca ccgcaaagtt tggaaagtgt
     9601 tttatctact atgttgacga atgtcacaca aaatcctgtt caatctctta ttgatgcgaa
     9661 ttaccttggc gtcttattct gggctgtatt gttgggacta gcttttcgag cacgttcaga
     9721 atcgacaaaa gagttggtgg atcagatatc tatagcgttg tcacaagtcg tacaagtgat
     9781 tattgcattt gctccgatag ggattttagg attggtttat caatcaattg ctacgactgg
     9841 aatcgctgga ttggcagagt atctgcagct tttacttgtt ttgatcggta cgatgctgtt
     9901 tgtcgcatta gttgtgtacc cattcctcac gtttttgttt attaaagaaa atccctatcc
     9961 attgattttc ttttgtttga aagaaagcgc acttcccgct ttctttaccc gaagttctgc
    10021 cgcaaatatc ccgataaaca tgatgttggc tgaaagattg aaattgacaa aagagtctta
    10081 ttccatatcg atcccgttag gtgctacgat caatatgggc ggtgccgcga ttaccatttc
    10141 cttgatgacg ttgactgctg tacattcttt agggattgaa gcaccttttg ttttgaaagt
    10201 ccttttatgt attttgtctg ctttagctgc ttgtggagct tcaggtgttg cgggcggttc
    10261 actgttattg attccattag catgtagttt gtttggtatc tctaatgatg tcgcgatgca
    10321 aatcgtcggt attggattta ttatcggtgt tatccaggat tcaatggaaa ctgccttaaa
    10381 ttcgtcaagt gatctgttgt ttactgcaat aggagagcta ggcgcgcgta aaagaagtgg
    10441 ggaaaaaatc aaattgaaag attgtttaga tggattaagt gaacgttgaa cgttagatat
    10501 ttagtatttt taaaatgatt cgagttttta atgagttata tagatgttcg tgtaaataga
    10561 ggtttctgtt aaatcaagat aaagtcgttt ataataacaa ttaaaagcat aggtaattgt
    10621 tattggaggc gtatcatgga ttttttacgt aaatttggtt ttttcttttt attattaggc
    10681 gtgctgagtg attttttgac tccgtacgta ttaggattgt attatcctga attagatcaa
    10741 atgaagagcg tgatcagtgt atttggagag gtttccagtc ctgtaagaaa agcgtttctt
    10801 atttggtctg tggtttcagg gatctttttc atcctgtcat tgcctgcatt gtatgctgca
    10861 tttgttagta catctcgtgt gttagctacc ctagttacag cagccatcgc gttctatggt
    10921 ataggtgatt gtatctttac aggtttgttc agtatcaata cccatgaatc ttcgtggaat
    10981 gtctcgacat gggtccataa tatcggatca ggtttaggtt acagtggttt tttattgttt
    11041 ccagttcttc ttttcttgat ctatcgaaaa aatgggcaaa tggacgtaag ccacttatac
    11101 cttggactgt ctgtgatcag tttgcttttt gcggcgattt atggactggc aagaattcct
    11161 tctataaatt cttggccatt actgaataaa ataggttttt gccaaagaat cagttttttc
    11221 tttaattatg tgcccattgc ctggttgagt atcttgcatc tcaaacagta aaacttgagc
    11281 cgtcaaattg aacagtcagc ggatacttca tccggtggct gttttttgtt tttctgaaat
    11341 atatatttcc acagttttct tgtatagctt ctgtcttaga gaagcaagga gctatatttt
    11401 gtgctacaat cttaaaaagg tagagagaag accaaatgga agacagagga gccactaaaa
    11461 tatgagaaac atcaagttga caatcgaata tgacggaaaa agatattcag gatggcaaag
    11521 acttggagat gacgataaaa ccattcaagg aaaaatcgaa aaagtattgc atcagatgac
    11581 aaatgaagag atagaaatta ttggatcagg aagaacagat gctggcaccc atgcgcgagg
    11641 acaagtagca aattttaaaa caaacacaga aattggactg gcagaaatga tcgactttat
    11701 gaatcgttac cttccgcggg atattgtcat caaaagaatc gaagaaatgc ctgagcgctt
    11761 ccattcaaga tataatgcgg tgggcaaaaa atacagctat tatgtttgga acaacgtgat
    11821 tccttcagca tttgagcgaa accacagctt ttatttccct caagagttgg atatggacaa
    11881 gctgaatgca gcttgcgaaa aattagtggg tacacatgat ttcattggtt tttctgcatt
    11941 gaaaaaatca aaaaaatcca cagtgcgtac gattgatgaa ttatctatcg aacgaaaggg
    12001 agaaatgctt cattttactt ttgtcggaga cggattctta tataaaatgg tgcggattat
    12061 catggggaca ttactagaaa tcggcacagg tgccatgccg atagaaacaa tcgatgaaat
    12121 atttgaaagc aaagtgcggc aacatgcagg agaaaccgtc cctgcacaag gtctgttttt
    12181 ggatgaagtt tattatcgat aaaaatactt tgataatgga tgaaacgcat gtttcacagt
    12241 ataaatatcg atgttaaata atgtatgaag caaattaaag aaatataaaa aaagactttt
    12301 tagcctgttt atgtgtacag ctaaaaagtc tttcttaaat aaaaatctag tgagaatgtt
    12361 gtacttctct tctcggttgt ttttgtttag aaaaagtaaa tcgataaagt aaaaataaaa
    12421 tcaaagtaac gatagataaa tatcttgaag caattgctag ttttgtgtac tgataagtaa
    12481 taacgacttt atttatacct ggaggaatag ttaattcagt tgtgccaaac ttagataatt
    12541 ttgtttgtac tagtttgcca ttaatagaag ccacttgccc tttataacga taaataggtg
    12601 tcgtcaagac agtattttta ttattcgaat tatctaattc aattgtaagt ttgttactca
    12661 taaaatgagt tgttggattt atttcctggt tacctagtaa atattgatca ttattaatca
    12721 tatcaggatg atagattgac tctttgtttg cataatcagt atgattgtaa ttttcagcta
    12781 tagcaacagc gtcttcaggt gctataagtg ttaatgaatc tttgttagca tggatagaat
    12841 tgcttactcc tgagagatgc aatattagtg caagaaacgt aaagaccatt attttccatg
    12901 aattattttt agtagaaaaa atataataga tcactatact catcgtatag gctataaata
    12961 aagaagaaaa accatttaac cgccagacaa attgtagact cttaactggt gtatggttaa
    13021 aaagttgcca aggaaataaa ttggttgaac aaaaaagaac aaatacacca agaaagaaaa
    13081 taaataaatc atcagcagtt aattttttta taaaaataaa agcaccaatc aatccgagaa
    13141 ataagaataa tccaatagta tagttgttat agttattatt taaaatatgt gtcagcatat
    13201 cactaggagc cataccactt aattcttttc ccagtgggac tttcaattct tgtgctctag
    13261 attgttgaat aaaaggaatt aagaatcccg cacttaaaaa aaaggtgagg acggtagctt
    13321 ttaaaagact taagagtcta gcgatttttt ggtcccaaaa ataaaaagaa agaaacaacg
    13381 ttcctccaat aaatacagaa accatagcga cagaaagtaa atgagtatac actactagcg
    13441 tcatgccaat agacaaccag taccattttt ggtaatctct aatataaatt tcgtaacacc
    13501 ccattagtat caaaggcaaa aatgttaaag cgacagcttc tcccaatgaa gcacgatgga
    13561 atatgtcaat ggcacgatag gcggaaaatg tataaataat cgaaaataac agcgaaatat
    13621 aacgattgtt ttttatttgt ttcatagaaa aataagaaac gaccattgtt aaaaaagtga
    13681 taaaaaaata gtag
//
LOCUS       DACCXO010000071        13675 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.072, whole genome shotgun sequence.
ACCESSION   DACCXO010000071 DACCXO010000000
VERSION     DACCXO010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.072"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..901)
                     /locus_tag="IVY70_001889"
     CDS             complement(<1..901)
                     /locus_tag="IVY70_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP7959013.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(925..969)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1184..2152
                     /locus_tag="IVY70_001890"
     CDS             1184..2152
                     /locus_tag="IVY70_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7959014.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2265..2543
                     /locus_tag="IVY70_001891"
     CDS             2265..2543
                     /locus_tag="IVY70_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP7959015.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2661..2897
                     /gene="secG"
                     /locus_tag="IVY70_001892"
     CDS             2661..2897
                     /gene="secG"
                     /locus_tag="IVY70_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP7959016.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3009..3755
                     /locus_tag="IVY70_001893"
     CDS             3009..3755
                     /locus_tag="IVY70_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7959017.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3758..6115
                     /gene="rnr"
                     /locus_tag="IVY70_001894"
     CDS             3758..6115
                     /gene="rnr"
                     /locus_tag="IVY70_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP7959018.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6137..6601
                     /gene="smpB"
                     /locus_tag="IVY70_001895"
     CDS             6137..6601
                     /gene="smpB"
                     /locus_tag="IVY70_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP7959019.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6903..7622
                     /gene="atpB"
                     /locus_tag="IVY70_001896"
     CDS             6903..7622
                     /gene="atpB"
                     /locus_tag="IVY70_001896"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP7959020.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7675..7890
                     /gene="atpE"
                     /locus_tag="IVY70_001897"
     CDS             7675..7890
                     /gene="atpE"
                     /locus_tag="IVY70_001897"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP7959021.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7981..8505
                     /gene="atpF"
                     /locus_tag="IVY70_001898"
     CDS             7981..8505
                     /gene="atpF"
                     /locus_tag="IVY70_001898"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP7959022.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8492..9034
                     /locus_tag="IVY70_001899"
     CDS             8492..9034
                     /locus_tag="IVY70_001899"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP7959023.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9057..10613
                     /locus_tag="IVY70_001900"
     CDS             9057..10613
                     /locus_tag="IVY70_001900"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP7959024.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            10627..11529
                     /locus_tag="IVY70_001901"
     CDS             10627..11529
                     /locus_tag="IVY70_001901"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP7959025.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRAVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11552..12958
                     /gene="atpD"
                     /locus_tag="IVY70_001902"
     CDS             11552..12958
                     /gene="atpD"
                     /locus_tag="IVY70_001902"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP7959026.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12972..13394
                     /locus_tag="IVY70_001903"
     CDS             12972..13394
                     /locus_tag="IVY70_001903"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP7959027.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4521 a         2447 c         2980 g         3727 t
ORIGIN      
        1 tttcggaaaa aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc
       61 aaaattcata acgatcaacc aatgtttgtc cacgacttat tccctgagtt tccacagcgg
      121 tgaagctttt gaaaccttca cagatggccc cctcgatgaa ataagcaaca gctaacggat
      181 cattgatgac acatcctaaa attctttctt gtttccagtg gaagtcaaaa taaaaacgag
      241 tgatcgcttt taaatactct ccctcttcag gattcatttg acagcagtat tcgaggatag
      301 tcggtgtcaa gacgatttct cttgtcacat ctaacccaac catttctatc gtttgtttga
      361 gattctcgaa tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg
      421 gcgaacagtt accatggctt ttgtaagttc cacccatcga gacaaaacga tccatatgtt
      481 tcccaatatt tgggttgact ttcaaagcaa aagcgatatt ggtcaaagga cccaaagcaa
      541 tgactgatgt atttgtcttt tctttgaaat aatcagccaa aaaatcaacg gcatggagag
      601 gttctgcctg ttttctcaag atcatcggga aatttgtttc ccctaaacca tccataccat
      661 gagtgtcttg tgcactgatg aaaggttgct tcaaaggttt ttccattcct tgataaacag
      721 gaatgtctaa acgatcacag cgttccaggc atttcagcac attctgcgta cctatatctg
      781 cagggacatt cccacaaact acggtaatag caactacttc gatatcaggg gatttgacag
      841 ctaatgataa agctaaagtg tcgtcgatcc ctggatcgca atcaataatt acttttcgca
      901 ttatttccaa tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac
      961 cagtccaaga cgagcgtctt gattataatg cacgaaatta gtatactggc ttttattgcg
     1021 aatgtaaatg ctctcttaca agtaaatcta ttgaggagaa ctttaagtaa atgatacaat
     1081 gaaaaagaaa tggaatttac gtgtgccgaa aataaagaat cctatggatc ggtacattgg
     1141 ataatagata gattatagtt tggcagaaag cggcgggtgt acaatggtta ttttttattt
     1201 cattcttatt ctgataatta tagggatcag ttaccatttt ctttcttatt tttatgcaaa
     1261 gaaattagta agagcgggag tacagagagg atatgactgg tatgaagaca ctggtcatcg
     1321 tttgatggac cctgctgcag tcacgtcagt agaaaaaaaa cgacaaaaat tagaagaaga
     1381 gtgtgaaagc ttttgggagc agggaacacc caaaacaatc aaaagttatg acgggttgaa
     1441 actagctggt cagatgttca tccatccaaa taagcagaat aaatgggtca tttgcgtaca
     1501 tgattaccga agcacaggga aaagagacat gtctcatata ggtaaaagat atgcagagaa
     1561 aggattcaac gttcttgtac ctgatttacg tgcgcacgga gaaagtgaag gagaaatcat
     1621 cggaatggga tggctagatc ggctagattt aattgcctgg atccaactca tcttagatga
     1681 acagccgaat gcgtccatta ttttacacgg cggatcaatg ggagcttcta cgatcatgat
     1741 ggcaagtggt gaaaaacttc catcagcggt caaaggattt attttagaca gcggatatgt
     1801 ttctgtttat gcagaattcc gatatatgtt aagtaaaatc actgtctttc ctaaaaaaat
     1861 ggtcatgaga tatgcgaatc attatgccca gaaatacgcc ggttactcct taaagcaagc
     1921 atcagctacg agacagcttg gcagcaacca tctgccttta ttagtcatcc atggtgaaag
     1981 agatcatttt gtccctacag aagcagcgta cacgattcaa aatgctacgg caggggataa
     2041 agcactgctg cttgttccag aagcagaaca cttggaagca tcgatgaaag atccgaaaac
     2101 ctattggaca gtgatttttt catttattga acaaagaata acgatggatt aattttctct
     2161 tcttttttgt aagtaattcg aggataaata attgtttata gatctggaga ctgctaaaat
     2221 aaggtaggaa taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagaag
     2281 aaaaaaatcg tcttgcttta ttcaatgatg agcaacaaga gattggagaa atgacatggt
     2341 cagatgcggg cccagacatc atgatcattg accatacatt tgtagatcca gcatatcgtg
     2401 gtcaaaagtt agctgaaaag ctagtgtata ctggagtaga attagcaaga cgagacggga
     2461 aaaagatcat tcctttatgc ccttatgcaa aaaaagagtt tgaaaagaaa ccagaatatc
     2521 aagacgtctt acgtcagtca taagaataac agctgaaagt tttgaacttt cagctgttat
     2581 tccatttata tagagttgtc acggactttt atctatgata aagttaaaga gacgatatct
     2641 tttgggaagg aggctgcgat atgtataatt taattttggg aatcgtgatc gttttatcga
     2701 ttgttatggt cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat
     2761 ttaccggcgg tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca
     2821 tgcagcgatc aactgcagtt atgggagcta tctggatgat cttattattt gtcctagcat
     2881 atttatcttc gaagtagttg aataagcctc tcgcaaaacg gcgggcggct ttttttattg
     2941 caaagagaac gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg
     3001 tgagacgagt gagacaatta ccaaaacccc tttatgcaaa acatggaaag cgagcggttt
     3061 tgctgcttca cgcttactca ggaagtccaa acgatgttcg aatgctcgca cgatttttgg
     3121 aaaaagcaga ttatacagtt tatgcgccgc tttttaaagg acacggaaca atggagccat
     3181 atgatatctt gcaagaaaaa gcagaaagct ggtgggcaga cactaaaaaa gccatccatt
     3241 ttttacagtc tgagcagttt tctgatattg ccgtattagg attatcaatg ggcggcatct
     3301 ttgctgtacg agcactagaa gaagaatcag tgattggcgg cggttttttc tgttcgcctt
     3361 tgtctcctgt aaaaacaaac gtgcctgaaa actttgaaaa atacgtacgt caagtgctga
     3421 agacagcagg taaatcagag aaagaaatca atgaaaaagc agttgcttac cgctcgttag
     3481 ctgaacaaca attaatggat atacaagatc aggcagcaat cgtagaaagt aggttgtcag
     3541 atatccaaca gccgatattt ttagctcaag ctgggaaaga tgaaatgatc gaccctaatg
     3601 gcgtatttga aactgcacgc aagttgagcc gtcagcgagt gacacttcaa tggtatcctg
     3661 aaagtggtca tgtgattaca gttggtacag caagacgcga attagaaaaa gacgtcttgg
     3721 aatttttaga aaaattacct tggaatgagg aataaatatg acaaaagaaa cgataaaaga
     3781 gaaaattctc tttttcatgg aaagcagtcg caaacgcagt ttttctatgg aagaaatcgc
     3841 tgaaggttta gggttccaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca
     3901 aatggaaaga gaacagagta tagttttcaa taaaaaagga aaagtgaaac ttcctttaaa
     3961 accaatcttg atcgaaggaa catttcgagc aaatgaacga ggatttggat ttgtaacgat
     4021 tgatcccgaa gaagatgaca tctatatccc aaaagaagcg actggttttg caatggatgg
     4081 agacaccgta gcaatcgata tcatgaagcg agcaaataca gatatggatc gcggagcaga
     4141 ggggaaagta gtcgagattc gagaccgggc gactactcag ttagcagggg aattcgttgc
     4201 ttattcagaa gaagaaatac aagaaacaga tctttacggc gttgttatcc cgaaagatag
     4261 aaagctcaac caatttaaag tatatgcagc agcagaggga gttcgtccag ttgacggaag
     4321 tattgtgctg atcgaactga cccattaccc tgaaaaaggg tatgcgacta gtttagaagg
     4381 gataatcaag caagtgattg gtcataagaa tgaccctggc atggatatcc tctcaattgt
     4441 cgtggctaat ggtatcccta cgaaatttcc tgatgatgta ttgtctgaag cagatgaagt
     4501 tccagatcaa atcagcgaaa aggatataac tggacgtaga gatttaagaa atcagctcat
     4561 tgttacgatt gatggagaag atgcaaaaga tctagatgat gcagttacgg ttaaaaaatt
     4621 agataacggt aattattttc ttggagtaca cattgcagat gtttcttact atgtgacaca
     4681 aggcagtcaa ttggacagag aagcatatga aagagggaca agtgtatatt tgactgatcg
     4741 agtgattccg atgatcccgc aacgtttatc gaatggtatc tgttcactga atccccatgt
     4801 tccaaggctg accatgagtt gcgaaatgga gattgatcca aatggacaag tagtctctca
     4861 tgatattttc ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca
     4921 aatcttagaa gatcaagacg aagaaacgat gcaacgctat gctcagctcg tacctatgtt
     4981 ccaagaaatg ggtgaattgc atcgaatcct tgaaacgatg cgaatcaaac gtggcgcgat
     5041 ttcttttgag gatcgcgaag cgaaaatcct tgttgatgct gaaggacacc caaatgacat
     5101 ccagctaaga agcagaggaa ttggtgaacg cttgatcgaa tctttcatgc ttgcggccaa
     5161 tgaaacggta gctgaacatt ataataaatt aaatctgccg tttatttacc gaatccatga
     5221 acagccaaaa gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct
     5281 ggtccgagga acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt
     5341 agaagacaaa ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc
     5401 acgctattca gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt
     5461 tacatctcct attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag
     5521 tgaagataaa agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga
     5581 tcatagttct aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa
     5641 gaaagcagaa ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt
     5701 tgtgaaattc ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa
     5761 tgaattgaaa caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag
     5821 aactggtctg acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc
     5881 agatgaacga gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg
     5941 tccaaaagca cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc
     6001 agaaaaaaat ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa
     6061 aaaagggaaa aagccatttt acaaagatgt caaaaagaag aaaaagaaaa aataaaatga
     6121 gttggaggaa acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc
     6181 tcgccatgat tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga
     6241 gatcaagtcg atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa
     6301 cggggaagct ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa
     6361 tcatgatccg ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga
     6421 aaacgaattg aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg
     6481 ctatgccaag gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga
     6541 tttgaaacga aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata
     6601 atttcattat cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt
     6661 tagcatttaa tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt
     6721 gcatggggaa aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa
     6781 aatgttttga attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga
     6841 aaaaaccaga tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg
     6901 gattggatga acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt
     6961 gtataatggt gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa
     7021 atcttaagtt gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc
     7081 gaggaatcgt aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat
     7141 ttacattgtt tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct
     7201 tacctaacga aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt
     7261 tggcatttat aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag
     7321 gatatttcgt aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg
     7381 aagaatttac aaatttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg
     7441 aagttttgct aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat
     7501 tagcaattcc attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag
     7561 catttgtctt tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat
     7621 aatactcggt taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat
     7681 tatatcgcag cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt
     7741 caagttatct ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc
     7801 cgtacaacaa tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt
     7861 atcgccttga ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc
     7921 gtttgttact gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct
     7981 atgctgaatc aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt
     8041 gtcagcggct catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc
     8101 agcgatattt tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa
     8161 tctcgcatca actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt
     8221 tctgatgcag ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat
     8281 attttgaagg atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc
     8341 actgtagaac gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt
     8401 caaatcgcgg aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc
     8461 aatcaataca ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag
     8521 acgttacggg aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta
     8581 tcaagatctc gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt
     8641 aagtgatgtt cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg
     8701 ttatgatggg atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga
     8761 ccttttgttc atgatcgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt
     8821 gggaagtgtc acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa
     8881 tgttgcaaaa acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat
     8941 tattggtggc gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca
     9001 attagaaaaa atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg
     9061 ccatcaaagc agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg
     9121 tgctttcagt cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac
     9181 atggtttaga aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa
     9241 tggcgcaaaa cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca
     9301 ttcgtgaagg agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg
     9361 ccttgatcgg tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg
     9421 ttactgacaa agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg
     9481 ttaacgaacc aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg
     9541 gacaacgtga attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgatcgata
     9601 cgatcatcaa ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag
     9661 attctacagt tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa
     9721 tcgttgtgaa tgccggtgcg tctcaaccag caccattgct ttatatcgca ccatatgctg
     9781 gtactgcaat gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg
     9841 atctttcaaa acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag
     9901 gtcgtgaagc ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag
     9961 caaaattaag tgatgattta ggtggcggtt caatgactgc cttaccattt gttgaaacac
    10021 aagctggaga tatctctgct tatatcccaa caaacgttat ttcgatcact gatggacaaa
    10081 tcttcttaga aagcgacttg ttctacgcag gtactcgtcc agcggttgat gccggactat
    10141 ctgtatctcg tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt
    10201 tacgcctaga cttagcaagt taccgtgaat tagaagcatt tacacaattc ggttctgact
    10261 tggatgcagc aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac
    10321 aaaaattgca tgcaccgcta gctgttgaaa aacaagtagt gattttgtat gctttgacac
    10381 atggattctt agatagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg
    10441 aatatctgga taccaatcat tcagatatct ttgaaacaat ccgtacaaca aaagatcttc
    10501 cagaagaaga agcgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa
    10561 aaggaagtaa ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg
    10621 agatgaatgg gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg
    10681 agccaaatca cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct
    10741 tcttcacaga agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact
    10801 gctactcagt taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc
    10861 atgctgctca gccgtgctgt gaagaaaact ggttatatcg tcatcactgc agatggcggt
    10921 ttagtcggtg gatataacag ctcgatattg aaacaaacga tgtctatttt agaagaagac
    10981 caccaatcac ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt
    11041 aaagcacgcg gtatcaatct agcatatgag ctgcgcaatc tatctgatca gccaagcttt
    11101 gatgaagtcc gcaaaatcgt aaatatggca acaactatgt tccaaaacga agtattcgat
    11161 gaattatacg tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa
    11221 aaaatgctgc caatctcaga cttagatcca gaagaagcaa cgactttcga acaagaatat
    11281 atttttgaac cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt
    11341 ttgatctacg gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca
    11401 atgaaaacag caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac
    11461 cgtgcaagac aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca
    11521 ctagaatagt attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat
    11581 cggtcccgtt gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc
    11641 attagttgtt tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga
    11701 attaggggac ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg
    11761 aatggaagtc attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg
    11821 tcgcgtattc aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc
    11881 ggaaagaagc gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga
    11941 aattttggaa acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa
    12001 agtcggacta ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca
    12061 taatatcgcc caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg
    12121 tgaaggtaat gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat
    12181 ggtgttcgga caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt
    12241 gacgattgcg gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa
    12301 cattttccgt ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc
    12361 tgcggttggt tatcaaccaa cattggcaac agaaatgggt caattacaag aacgtatcac
    12421 atctacgaaa aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta
    12481 cactgacccg gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg
    12541 taaattaaca gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc
    12601 tttagcacct gaaatcgtgg gggaagaaca ttataaagta gcaacagaag tccaacacgt
    12661 attgcaacga tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc
    12721 cgatcaagaa aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa
    12781 ctttaacgtg gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac
    12841 agtgaaagga ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt
    12901 ccgaagtgtt ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa
    12961 aggggtgccc catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct
    13021 atgatcacca tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc
    13081 ctaaacatgc accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag
    13141 attcagatac ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata
    13201 atgttgtttc aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg
    13261 cggaacgtgc aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata
    13321 cgaacgaatt gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat
    13381 ctaagcactc ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg
    13441 ttatgacaga agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa
    13501 atttttgtga aatctcagct tatttttcga agaagctact tctggaacac cgtatatcag
    13561 aggttgtaaa atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc
    13621 atattttgcc gttttttgag ctaataccga gggtcagatg attgaacacc gttta
//
LOCUS       DACCXO010000072        13476 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.073, whole genome shotgun sequence.
ACCESSION   DACCXO010000072 DACCXO010000000
VERSION     DACCXO010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.073"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..165
                     /locus_tag="IVY70_001904"
     CDS             <1..165
                     /locus_tag="IVY70_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP7959028.1"
                     /translation="KKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEENR
                     KKAMRKRVSR"
     gene            complement(227..2038)
                     /locus_tag="IVY70_001905"
     CDS             complement(227..2038)
                     /locus_tag="IVY70_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP7959029.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2035..2400)
                     /locus_tag="IVY70_001906"
     CDS             complement(2035..2400)
                     /locus_tag="IVY70_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP7959030.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2563..2958)
                     /locus_tag="IVY70_001907"
     CDS             complement(2563..2958)
                     /locus_tag="IVY70_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP7959031.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2976..3938)
                     /locus_tag="IVY70_001908"
     CDS             complement(2976..3938)
                     /locus_tag="IVY70_001908"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP7959032.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3938..4150)
                     /locus_tag="IVY70_001909"
     CDS             complement(3938..4150)
                     /locus_tag="IVY70_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP7959033.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4171..4869)
                     /locus_tag="IVY70_001910"
     CDS             complement(4171..4869)
                     /locus_tag="IVY70_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP7959034.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4889..5431)
                     /locus_tag="IVY70_001911"
     CDS             complement(4889..5431)
                     /locus_tag="IVY70_001911"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP7959035.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5563..6567)
                     /locus_tag="IVY70_001912"
     CDS             complement(5563..6567)
                     /locus_tag="IVY70_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP7959036.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6564..7553)
                     /locus_tag="IVY70_001913"
     CDS             complement(6564..7553)
                     /locus_tag="IVY70_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP7959037.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7550..8356)
                     /locus_tag="IVY70_001914"
     CDS             complement(7550..8356)
                     /locus_tag="IVY70_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7959038.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8522..9478
                     /locus_tag="IVY70_001915"
     CDS             8522..9478
                     /locus_tag="IVY70_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7959039.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9555..10073)
                     /locus_tag="IVY70_001916"
     CDS             complement(9555..10073)
                     /locus_tag="IVY70_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7959040.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10161..10310)
                     /gene="rpmG"
                     /locus_tag="IVY70_001917"
     CDS             complement(10161..10310)
                     /gene="rpmG"
                     /locus_tag="IVY70_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP7959041.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10538..10984
                     /locus_tag="IVY70_001918"
     CDS             10538..10984
                     /locus_tag="IVY70_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP7959042.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11179..13074)
                     /gene="asnB"
                     /locus_tag="IVY70_001919"
     CDS             complement(11179..13074)
                     /gene="asnB"
                     /locus_tag="IVY70_001919"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP7959043.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4201 a         2854 c         2299 g         4122 t
ORIGIN      
        1 aaaaaatttg tatctggaat attagtagga agtctggcaa cagccgctgc cgttgctggt
       61 ttggtcgctt ctgtgaaaaa aacagtgatc gatccaattg acgaaaaaga agcaatgatc
      121 gaagaaaacc gtaaaaaagc aatgcgcaaa cgcgtttctc gttaataatt atttaaaaac
      181 acgcatgaag acttgtgaaa gaacagataa gtcatcatgc gtatttttat ttgtcagtca
      241 atataagttg tgctatttta tctgcctgtc tcagattcca tggatctgta taaggtctag
      301 ctactgctgc agttagccaa tccacacctt caacaggtat tccatagcaa taaaagtcag
      361 actggatttc ccctgaagca tcaatcacgc gattcgtacc tcgctcaaca tctatagctt
      421 ctgaatgata acttcctgta ttatctgaaa atgaaaaagg gcgaacaagt ttgttttcct
      481 tcaaattttt tatgacagga ttacttgaac gagaaaagct tgtttgtggc aatcgagctt
      541 caatcaaata gcgactgctg atttctttat cttgtagaga aataacatag cggttgtgct
      601 tttgctgaat atcaaatggc ggttcgaaca aatgaaaaat acccgcatct atcagtgctg
      661 ctaattcttt cgtccgaatg actggcggac cgatcgttaa aaagctattt agatgagtaa
      721 accatcccca tagtatttct ttgtattcct ttgcagaaaa aacgtgccat tcaatagcct
      781 gccggattgg atcacgccag tcttttaatg catcaaatgt tgaagcaaat gctcctgtgc
      841 aattgccttt ttccgcttct tttatctgcc cttcaataaa tgctcggcta gcttctgcaa
      901 aggactcatc ctgataggta agaggaattt ctaaatacga ccaatcccat ctataaggga
      961 ttagtgctgg atgctttccg agacactcct ctgaagagag ggagagaaaa tcatgctcaa
     1021 aatttttctt tggaatcggc aaatgatatt cttcgatgat tttcttgtaa taaaacaact
     1081 caacgtcttt tttcagatag tcaaaaaatg tttcgccagt caaaaatccc gactgatgcc
     1141 attgctccag gttttctttt gttagtaacc gtggccatgc cattgatccg ccttgctttt
     1201 ggtttcttcc tcgagggtaa taagggagcc cccttcttga tccactgtat acaatcggtt
     1261 ccttgcctga tggatgatag acaagttttc ctttttcgtt ctcgaagcgc ccttctcttg
     1321 atactgtaaa caatccgaca taatcaaaaa aacttaagcc tagacctctt aaaaagacat
     1381 tttctttagg cggtatcttc gaaagatcga catctgcagg attagaagga agctgataca
     1441 gtaaatcatt tttctttgcg tagtctaccc acactctttc ttcttctgtc tcatcatttt
     1501 cccagtgacc gcttgctaaa acaagctgat cgatagaata gctttgctga tctgtctgta
     1561 aaagataacc atcttttctt ttgctgactt tttttactaa agaataaaca aactgaatag
     1621 aagctggaaa ttcttcttgc aactttgaga aaaaccagcg ttgatagagt ccgtataaac
     1681 atcgagtaga ttgctgatct ttttccagac gattgatttc ttctataatt agttgcttat
     1741 ttttggttac cgtctgttcg atataatctg tagcaggtcc tttactccat tgaaacaagt
     1801 tgggtccctg tgttaccaat cctccactgg acaatgtttc atctgtaaaa agcgtcactt
     1861 gttgagaaac agaattcatt aataactccg gtggctgatt caaccgccaa actcgacctc
     1921 caggaccatt gggatcgaaa agaaaaagtt caagagaatg tctgtcctga ttattatgga
     1981 gaatcctttc tgctatactt aatcctctag gtccggcacc aatgatccct attttcattt
     2041 gcatgcctcc atttcgataa attttttcaa taaaacagca tggttctgat gttcattttt
     2101 tgcaccataa acaaaagtaa tgactggatg ttctttcata agttgactga gtttaaaaat
     2161 agcttgcttc ttttcttctt cgaaaatcaa ttcttgctgg tatttctttg caaatgaagc
     2221 aaatcgttct ggtacatggt caaaagtttt gcgtgcttca ttactaggtg caatttcttt
     2281 caaccacaga tctaactgcg ctttttcttt tgaaattcct cgtggccaga acctatccac
     2341 gagtattcta tagccatcct ctttttcagc atcaaggtaa acacgtttta atttgatcat
     2401 agttgtttcc ttctttctgc cttttcgctt agcttatata tgtagataat agcacaaaat
     2461 aaaaaaaatg aacggcacaa ctatccattg cgccgttcat gtgatcttgg tcaaaatcta
     2521 atgattgatt cggtttttaa agctgtcata caatcatctg ttttatactt ttttactttt
     2581 gatttttcca gaccatccgt catatccacc cttaagtaaa taaatctctt gataacctgc
     2641 tttacgtaat ttatttgctg tacggatgct caagcttttc ttttgatcgt aaatatagac
     2701 aggctggtct tttcgaatag atcctaatga atctttcaaa acagtgtaag ggatgtttct
     2761 tgcacctaaa atatgcccag catcaaacac atctttttcg cgaacatcga ttacttgtgc
     2821 ttttctcatt ccttctcgaa attcttcttc tgtcaaagtc gttgctgaac gcttggccat
     2881 tactcgtaga tagacttccc aaaagatgat tgcaagcaca atcaatagca aaatgatatt
     2941 aattacccat aaaaccatgt aatgcctccg tttttttaag caaatttcaa ggctaatgag
     3001 gcagcaccaa tgacaccagc ttcattacca agctctgcta atttgatttt tgttgattct
     3061 cttacttgcg ggaaagtaaa ttctttaaaa tatttctcta cacggctacg taaaaattct
     3121 cctgctgccg atactcctcc acctaacaca atacttgacg gattcaatgt attccccaag
     3181 ttggcgcaag ctaaacctaa gtagaagcat acgcgatcaa caaccatcaa tgcaaacgga
     3241 tcgtcttctt gagccatttc aaagatatct ttacttgtta cttcttcacc gttgtctaac
     3301 attgctttca gttttgaatc tcctgcgtat tcttctgcca agtaacgacc tagacgtacc
     3361 acaccagtag cgctagacac agtttctaaa catccgcgtt taccgcaagt acattcgaac
     3421 ccttcaggat ccacagtgat atggccgatc tctccgcctg ctccgctcac gccgtgaatc
     3481 aaatttccgc cagcaacgat accaccgcca acacctgttc ctagagtaac gaatacaacg
     3541 tctggatcgt tttctccagc acctttccat cgttcaccta aagcagcaac attcgcatca
     3601 ttatcgatag cgaatttgat tcctgttcct gcctcgatca gttttttcac aaattgaact
     3661 tctgtccagt ttaagttata cgcaccaata accgttccag cttcgctgtc cacgcttcct
     3721 ggggtaccca tgccgattcc gataaagtct tcggctttca tattatataa attcaaacga
     3781 tggttgattg attcaatgat ttcaggaacg atatgcttac catcatccaa aatattggta
     3841 tcgatgctcc atttttgttg aatctctcct tctggtgtta aaattgcaaa ttttgctgtt
     3901 gttccaccta aatcaatccc aataatcttc ttgtccatta caaatttcct ttctttcggc
     3961 tttcttcaat tcggtgctct ctttttaata tgcgccacgc tgtcatgtat gtatcatgat
     4021 caatcaaacg gctatcatac agattacgca attcgatagt cattaattca atgtcataga
     4081 tgcgtgcacc cacatagata aagatgccaa attttttgaa cagctgctgt acatcatata
     4141 agctctccat ctttaagcac ttccttcata ttatacaagt aaaccatatt tttttaaacc
     4201 cagtaaaata catattacaa aaagaaatac aaaaactagt gtagaaagaa tcctttggtg
     4261 tatcgaatag cttgtctgtt gtttcggtag acccaaaaca ttccctaaca ctaatccgcc
     4321 aattagccca ccgacatgtc cccaaacatc tacagaggta tccagaagac caaaaacaaa
     4381 tgtcaacaca ataaataaaa gaaaatgtct gactagctgc tggatcgctg ggttatgttt
     4441 aaaatgaaac cctaagataa acaacgcacc gaacaaaccg aatatcgaag tactcgcacc
     4501 accagacaga acattcgctt cgttaaaagc aaagcttgct gtatttccca ttatcccgct
     4561 gaacatataa atcaagaaaa aacgccaatg gccatagatt gcctcgattt gctgccccat
     4621 aaaatacagt atcagtgagt tcattacgaa atgcatcagt ccaaaatgga tgaagattgg
     4681 tgtcactaaa cgccaccatt cattgaagtg aacaagataa ggaccataca ttccggcata
     4741 ataagggatc tcaagtcgcg gaaataattc catcatgagg aagaccgctg tctgtatccc
     4801 taaaaaagca taggtccata acggtcgtct taaccatgca cgcaatttta tttgctgttg
     4861 ataattcatg aaaacctccc atttcatttt agtcaataaa caattgttgg atcttctgat
     4921 caaagctctc cggttgccat tgttcattca attgttcacg aaaaacaagg ctacatgttt
     4981 ttttatgaaa attatttaaa taccggtcgt aataaccacc gccaaaaccg attcgataac
     5041 cggctctatt gtagataata cctggtacaa tgaggagatc gatcatctcc ggcaaataga
     5101 gcttctctga ttgtggctca agaacaccaa aactcgaacg tacaagaacg gactgttcat
     5161 ccatttcata aaacgccatc tgacgtttcg gcaaagtttt aggtacaact attttctttt
     5221 gttctttttc tgctcgttgg ataaccggct gtgtatccag ttcaaattca ttcgataaag
     5281 tcgtccctat cacttttgct gacttccagt actcgctggc gaatagctgt ttatagattc
     5341 gctgttcttt ttcctttttc ttgccacttt tggccagatt tttcagcttt tcgattcctt
     5401 tccggcgtaa tcttactttc aattggtcca aaaactcacc cttccttctc tcataaaatg
     5461 gtataggaaa aaaagaaaaa tagcaatcga actttcaaat aaatgcataa atagaataaa
     5521 aagaagacct agaaatgatt ggccttcctt ttttaataaa tttcaactgt tcagcgcgtg
     5581 tttttgtctg ataaaaagtg catacagaaa atatggcgca ccaaaaaggg caatcaaaat
     5641 tcctgtaggt acttccccac ttggtagaat cactctagca accatatctg cactaagcag
     5701 aaaaatggaa ccgaacaaac cgctcagaac aaaggtccag ttattttctt tacgtacgac
     5761 tagcttcgca gcatgaggag ctagtagtcc tacaaaagat aaactacctg tcaccgctac
     5821 actaccgctt gccaaagcaa cagctaacaa caaaaggatt ttttgacttt tagccacgtt
     5881 aaggcctaat ccttgcgcgc gttcttgacc aaatgatagc aatccaatcc tctgtgcata
     5941 aagtcctcct agcggaatta agataaccgc ccatggaagc aatgcccaaa cataaggcca
     6001 gctcgcaccc cagattgatc ccgctaacca tgcattcaca aatccataat tttccttaga
     6061 taattggatc gtgcatagca atgttaatgc agaaaggccg gcattaacgg caataccgga
     6121 caataagatt cgattcatac ccaaaaagcc atttcgatca taggccgcaa gatagacaca
     6181 gcaagcagct aaaacaccac ctagaaaggc tacaactagc aatagaaacg gcagcgaata
     6241 gtgcgaaaag aaacctaaat aaagcatcac taaaaatcct gctccggcat taatgcccaa
     6301 tatactagaa tctgccaatt catttcttgt tactccttgc aagatataac cagaaacgcc
     6361 aagacaagct cctgctaata aactgatcag taatcgagga actcgaaatt ctctaacaat
     6421 cagcgccaaa gaagatgatt ctttccctat gaacgtggca aaaagctgat ccaggtaaat
     6481 aaaaggcgta ccaatcatta aactacctat agacaggatg acaagtacaa aaagcaagaa
     6541 atagaacgtc caatatattt ttttcaccgt tgttccctcc ttacttgtat cagtaaaaac
     6601 ggaacgccaa tcaatgcaat gactaatcca aaaggtgttt caaaaggggg attgatcatc
     6661 ctcgctgtaa aatccgccaa cataaccaat ccaccgccaa ctaaaaaaga gacaggtaga
     6721 acataacgat agtccgttcc aaccattccg cgaacaactg gcggaaccaa cagcccaata
     6781 aatgaaacag gtccgactaa cgaaacggca atggctgcta acactgcaac tagacagaaa
     6841 acaatcaacc gcgttcgttt aggatgactt ccaagagcta atgcatgtgt ttcactcaaa
     6901 tgaatcaaag atattgcttt tcctaatgaa aaagaaacca aaacagcaag tatatataaa
     6961 ggaaacaata ctgctaattg tttccatgtg atattggcag ctccgcctac aaaccaaaaa
     7021 gcaagatcct gctgcagatt aaagaattgg gtaagtgctt ggctgatagc agtaaaaaaa
     7081 gaactaatag ctacgccagc taaaatcagc tgtaaaggat tcatgcccat tttcgaaaac
     7141 tgcaaaaaac caaatacgag caaagttaca gcaaaagaac ctgccaatga cattagaaac
     7201 acagaaaaat cagaagcttt aggccaaaaa gcaaaactaa gggccattgc caacgaagca
     7261 cccgcgttga ttcctagtaa gcctgaatct gctaaaggat ttcttgtcac tccttgcatc
     7321 aatgcaccgc tcaatgcaaa acaactccct acaagaaatg ctcccgccac acgaggcaaa
     7381 cgaatatttc gtatgatttg gtgactttgt tttgatctat caaaatcaaa caacgcctgc
     7441 caaaaatctg cttttcccac agtcgctgcg ccatttgaaa gagcaacaaa aaaaataatc
     7501 aaaagaacaa aacagaccaa aagcaggagt ctatttttct tcatagtcct cataattgat
     7561 cctcctgctc ttttggcaac aaatcatagg ttagaaaaac tggtttattt gttcttggaa
     7621 gcgtgacaat cgttgcatca atctcaaata tttttttcat acactctttt gtgatcactt
     7681 cttcaggtgt accttctgtg atcaacttac cgtttttcat tgctaataaa taatctgaaa
     7741 aacgtgaagc atgattaatg tcatgaatcg acatcaagat cgttgtttgt ttcatttgat
     7801 tgatttcagt caataactgt aggatctcta attggtgtgc cggatctaaa aatgttgtcg
     7861 gttcatccaa gatcaagata tctgtttcct gagcaagcgc catagcgatc cagacacgct
     7921 gggtttgtcc accagacagc gttgcgatat cccgcatacg aaatccagct agacctgtag
     7981 catccaacgc ccactcgatt gcttcatgat cttgcgtgtc catgccactg aacagttttt
     8041 gataagggta tcgtccgtaa gatactactt cttctactgt catacctgta gggtgttcac
     8101 tagtttgtgc taataatgta attttttttg caagttcttt tgaactgtat gtagagatat
     8161 cagagttatt gacaatgatc tttcctgatt tcacaggtag tatacgggta aaacttttca
     8221 ataaagtaga ttttccactt ccatttggcc caatcaaact agtgattttc ccttcaggaa
     8281 tcgtaacaga catgttttca atgatttttt tgttttcgta gccggctgca atgtcagctg
     8341 ctcttaatct cgtcatttca cccatccttt cgctgaaaat cattctcaat tatgacgctg
     8401 tttcaacatt tcgtcaagcg ctactttcat atctatagcc aaatgaagcg gttgacagcg
     8461 ttcgaaatat caggaataat aaccaatgag aaagattctc aattatgtta ggaggaaaga
     8521 aatgaaatta aaaacaaaag tattcacatt atctagcata ttgctgctgg ctggagtact
     8581 gagcgcttgc tccccctctt cagataaaga aacagctaca actgccaatt cgacgacttc
     8641 atccaatagt actcatacag tcacagatac acttggacat aaagtagaga ttccaaacgc
     8701 gcccaaacgg atcatcggca gttacttaga agactatctg attgcactag gggaaaaacc
     8761 tatcgcccag tggactgttg gcagtggttc cattcaagat tatctgcaag atgatttaaa
     8821 ggatgtccca acgatttctt atgaccttcc ttatgagaaa gtgttaagct ttgagcctga
     8881 tttgctgttg atttcttctt ccgctactgt cgaaggcggg aaatacgaac aatatagcaa
     8941 aatcgcgcca acttatgttg taaaaaacgg aacagatgtc acatgggaag aacaactaaa
     9001 agatgtcggc aaagctttgg caaaagaaaa agaggcagaa aaaatcattt ccgattatca
     9061 aaaatccatc aagaaaactc gagaagaact agcagataaa atcgagaaca agacagctgc
     9121 agtgttgtgg gtgaccaaca actcagcctt tatggtcagc aaaaatcggt caagtggaag
     9181 aatcgtatac gacgatctgg aatttggtgt tcctgatcta gtggaagagg tctccaaaaa
     9241 agccacttct gactggtctg ctgtttcctc tgaaaagcta gctgaattag atgctgacta
     9301 catcatcctt gtgaacagtg acaaagatgc tgccatgttc aatgaaccaa gctggaagaa
     9361 tcttaaagca gtgacagaca atcatgtact gcaattcgga cctgaatcta gttggctata
     9421 taacggacct attgccagtc aggaaatggt caatgatatc aaagaaaaac taaattgatt
     9481 ttaactaaaa aaagaatctt cacgattagt cgtgagatgc tttttttctt attaacaaac
     9541 tagacgcata gattttattc ttcttttgaa gcaaccctat tttttactct ttcccatttt
     9601 tgaaaacgat gggcatatag attttcttga tccgttatcc cctcatactc ctctataagg
     9661 gcgaagtctg aaaaatcaat attcccaata tacgtatcgc cttcaaacgt gctatcgatc
     9721 aatgttcgcc agatccgacc agtgtaagga agaagttctt gaaaaatccg actaccaccc
     9781 gaaacataga tatcttctga gatcgtttga ctatacgaca aaagttcatc gatcgaatgc
     9841 attacttccg cattttcttt cgtgcgaaaa tttggttgcg ttgtcaacac gatgatatgg
     9901 cgataaggaa gtgaaagatc ccccattcct tcataggtct ttcttcccat cactaaaatt
     9961 cgatccattg tatgttcgcg aaagaaacgc atatcatttg gcaatcgcca tggtaacaaa
    10021 ccatttttgc cgatcagacc atttttatct tgtgcccaca tggaaataaa catattatcc
    10081 gctccttatg attttagatg ttttgagata aaaaaagtct caattctttg accagcaagg
    10141 aattgagaac tttaaaaaca ttattttgtt tcacggtgca aagtaacttt gcgttcgcgt
    10201 gggcaatatt tttgtttttc caaacgatca ggattgttac gtttgttttt gcttgttaga
    10261 tagttacgtt ctttacaaga agtacactct aaagtgatgt ttacgcgcat gtttttccct
    10321 cccattttta caagaatcct ctaagatgac tctcagactt gattatctta ccatgatttt
    10381 ttctagattg ctagtgtttt tcacgatttt gttaagtaaa actacttgac gataaataca
    10441 acggttttac ttctcaaaca aaatcttttt ctttttgtat aaataccctt acacttgttt
    10501 atatagatta tgttttttaa taaaggggga tactaatatg ccatggaaca tgaaggactt
    10561 tcctgcatca atgaaaaact tagataaatt gacaagaaaa aaagcaatcg acatcgcaaa
    10621 tgcattatta gatgaagggt accctgataa ccgtgcgatc ccaattgcca ttgatcaagc
    10681 aaaaaaatgg tatgaagatg ctagtgaatc agaaaaacga actttcgaaa aagaaaaaaa
    10741 tccaagcaag acggatgaac acgatacgaa tcctcgtgct ggaaaactgc ttgattcaga
    10801 tgtgatcgta gaatatgagg aagaacaatg gatcgtgaaa tctaaagggg caaaaaaggc
    10861 aagcaaccat tttgatacaa aaaaagaagc aatcgaaaaa ggaaagcaag tagcacaaaa
    10921 caaagaatca actttgttca tttataaaaa agatgggaca aaagaaaaag aaatcgatta
    10981 ttgaacatat gtaaaggggt tgtgacgaaa atcgtgtcac aaccccctta tgttcgaaac
    11041 aatggtgttc ctctgccaac tccgaaccat aagtcataat atctataaaa cgggaagagc
    11101 tgttttttga aattgtttct tatcactcag tgttaaacgg taggagaaaa cctctttttt
    11161 attgatattt gattttcatc aaacagtcac atagtcgatt gcttctgctt gcggaatcgt
    11221 ttcatctaca aagtagactt tataccatgt caagaaactg aagattgtcc agatttttct
    11281 ttgctcttct ttacgtcctt cgtgatggtc atctagcaac ttcatgattt tttcttgatc
    11341 aaaaaattct tttgcaaaat ctgcttcgaa taatgctttg acttgttcgt agccgtgttc
    11401 ttctttcaac caagctttga ttggcacagg gaatcctaat ttcacacgat tcgaccattc
    11461 ttccggaaga tgtttgttcg cagcttgtct gaaaatatct tttgtgttgt tttgattcaa
    11521 taaaaactta cttggtattt gttgagccaa tttcatgact tcgatatcca ataaaggtac
    11581 tcggacttcc aaagaacttg ccatagaaag tttatctgcc tttaacaaga tatcttttgg
    11641 catccattga tgaagatcga catattgcat cttattgact tcatctttga ttccttctgt
    11701 tttctcgtaa tgaacagtca tgatttcatc cacagatggt gctgtttgat attttggtgt
    11761 taacacttct gatgcttctg attcttcgaa cactcgagca tgaccgataa aatattcttt
    11821 tgcaggagca agtgattcat ataaatggat ccgtccatgg aaatttttca tttttccgat
    11881 tttacgtcct aaattatagc gtgttccttt tggtaatttt ttcaatcctt gagcaatcac
    11941 acggatgaat tttgaattcg tatggaatcc atacgtttgg tatcccgcaa acagttcatc
    12001 cgccccttct ccagattgga ctacccgcac actttgtgaa gcaagatttg ctaaaaagta
    12061 caaaggtacg caagaaggat ttgaatcagg ttcatctaag tgatactgga tcaaagggaa
    12121 tgctttgaac gacatgtctc cgtcgatcac tgctgctgta ttatctaagt ccaatagttc
    12181 ggttagtttt ctggcttcaa tcgcttcatt ataggtttta tcatcaaacc cgattgagaa
    12241 agaatgatct ggtttcaata cagaggttac ataactggaa tcaaccccgc tggataagaa
    12301 agaaccaact tctacatcac tgaccgtatg tgcttcaata gaagcttgaa cggtttcgtc
    12361 gattttttcg atccattctt gtcgggaatg cgtttctttt gtttcaaaat ctgcatccca
    12421 atattgctga atgtctagtt ttccatcttt atacgtataa taatggcctt caggcaaacg
    12481 atagacatct ttgaagaatg tttcgccgtt taatggagaa tattggaaag tcatataagg
    12541 tttcaacgct tctttgttca attctttgtt aaaatcagga tgaggcagaa aacttttgat
    12601 ttctgaacca aacataaatg ttccgttcat ttctgcataa taatatggtt tgatcccaaa
    12661 gtggtctctt gcaccgaata actcattctt ttcattatcc caaatagcaa aagcaaacat
    12721 tccgcggatc ttttgcaata attctgttcc ccattcttcg taaccatgca aaaggacttc
    12781 tgtatctgca tgtgtttgaa aaacgtggcc agctgcaatc aattcttctc tcaaaggctg
    12841 gtaattatag atttcgccat taaaaataat gatctttgtc cgatcttcat tgtaaatagg
    12901 ctgagtaccg ccctctaagt caataatact taatcttctg aatcctaaag caacatgttt
    12961 atcgatatat tctcctgaac tattaggtcc acggtgaacg attcgatcca tcattgtatt
    13021 aataattgtt tttttgttgt ccttgtcgtt aacaaaacct acaataccgc acatttaact
    13081 ttcacatcct aatataaaat tactgtttat ttcattcata tgcatcttat catagaataa
    13141 atagcggacg aatattttct ctcttacaat ttcgtcacgt gaaaacaaaa gatgaaagtt
    13201 tctgttttta cagaaaataa agtcccaata tctaatttac catgttttct tttttgaggc
    13261 taccttattc tcttcgtgtt aaaaaaatat tagtggatgc tatttccaaa aaaaacagca
    13321 aaaaaagaca cgacctaaag atcgtgtcta attatgactg tatcagaaaa tatgatacca
    13381 aacggatcta ttcattaatt aattgacata gtttatctgt tgctctttta tgactggata
    13441 actgaccaat tcatgacgat ccaagtcttc tttatt
//
LOCUS       DACCXO010000073        13410 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.074, whole genome shotgun sequence.
ACCESSION   DACCXO010000073 DACCXO010000000
VERSION     DACCXO010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.074"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(48..590)
                     /locus_tag="IVY70_001920"
     CDS             complement(48..590)
                     /locus_tag="IVY70_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP7959044.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            725..1114
                     /locus_tag="IVY70_001921"
     CDS             725..1114
                     /locus_tag="IVY70_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959045.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1118..1882
                     /locus_tag="IVY70_001922"
     CDS             1118..1882
                     /locus_tag="IVY70_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7959046.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2011..2958
                     /locus_tag="IVY70_001923"
     CDS             2011..2958
                     /locus_tag="IVY70_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7959047.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3088..3828
                     /locus_tag="IVY70_001924"
     CDS             3088..3828
                     /locus_tag="IVY70_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959048.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4119..4787)
                     /locus_tag="IVY70_001925"
     CDS             complement(4119..4787)
                     /locus_tag="IVY70_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7959049.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            4921..5208
                     /locus_tag="IVY70_001926"
     CDS             4921..5208
                     /locus_tag="IVY70_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959050.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            5624..7162
                     /locus_tag="IVY70_001927"
     CDS             5624..7162
                     /locus_tag="IVY70_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP7959051.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            7159..8124
                     /locus_tag="IVY70_001928"
     CDS             7159..8124
                     /locus_tag="IVY70_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7959052.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            8111..9433
                     /locus_tag="IVY70_001929"
     CDS             8111..9433
                     /locus_tag="IVY70_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7959053.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      9496..9600
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9674..10417
                     /locus_tag="IVY70_001930"
     CDS             9674..10417
                     /locus_tag="IVY70_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7959054.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            10432..11424
                     /locus_tag="IVY70_001931"
     CDS             10432..11424
                     /locus_tag="IVY70_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7959055.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            11436..12173
                     /locus_tag="IVY70_001932"
     CDS             11436..12173
                     /locus_tag="IVY70_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7959056.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            12163..12885
                     /locus_tag="IVY70_001933"
     CDS             12163..12885
                     /locus_tag="IVY70_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP7959057.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(12969..>13410)
                     /locus_tag="IVY70_001934"
     CDS             complement(12969..>13410)
                     /locus_tag="IVY70_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP7959058.1"
                     /translation="ISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQ
                     ETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRY
                     QLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         4417 a         2294 c         2788 g         3911 t
ORIGIN      
        1 acatttaaaa tttaatttag atattagaca gtttttaagt ttgtgagtta ttttccttgt
       61 cgttttctca cttttttagt ccagaaacca cctgaaatat attttggctc ataagaaatg
      121 ataaaagcac gctcttcgat ttctttgatc aggcgataca gttcacgttc gctttttcta
      181 ggagaaagta tttcgagaac tagccgctca ccttccagcc ccattgcaga agtctgtgtc
      241 acaccataac cgtgttctct cagtataccc ggcaaacttt tatcttctgc gctatttgct
      301 ggcaaaatcg ttgtgaccat gatgtatcct aatgccaatt tgtcttcaat caaaattccg
      361 acactgatac ctactgcgta acctaacgca tagacaaata aattcagtgg attatctaaa
      421 cgatctaaaa caagggataa gccgacgata taaatcgtga tctcgaccat actgacaagt
      481 ggagcaatca gtcgatagcc tttcattgtc aacatgaaac gtaacgtgtt cagagtgata
      541 taagcaaaat taatgaggaa gatcatcccc aacatttttg gatcaatcat ttgtaaaact
      601 cctttcattt atataacaac aatatcaaaa aaaatcgtaa gtgaagaaaa attgaaactt
      661 tatacataca aaaaaatgat aaaatgatta taatagaatt ataaagatag gttaggtgat
      721 taaaatgaaa acggtcaatc gtgtgttttg tgccagccac gtgctaagtc tccttctcag
      781 tatatactta ggtgtctatt cgattcaaca gccaattgag gtagagaaca ttgtttttca
      841 gatgtcgctg acagatggtt taattttaag cgtttttttc tttttgatac tgtttattgg
      901 taatattttc ggtagcgttg ccagtttttt gaatttttct atttatccac tcctttcgct
      961 agcattagga gtggtgggac tttgttctct gtgcttattt cccgagccat tcagtcctgc
     1021 atttatatta tttggtacac tgaacctgtt ccagacaact gttggagcat ggttgctatg
     1081 gcgatcagga aatttaatga aaataggtga gtaacaattg aatccaatta tattgaaacg
     1141 ttctgtccat catattccag tgttagaagt tgttctgaaa gaattaagag aggaacgtat
     1201 tcccgttgtc atctattatc acggatggca gacaagtaag gagttagttt tgacgcaagg
     1261 aagaaaaatt gcgcagcagg gcattcgtgt gatcctgcca gatgcgatga atcatgggga
     1321 gcgcaaacag ccggtttctt ctattccatc ctatacattt tgggatagta tctacggaaa
     1381 tctgtttgaa tttgacacac ttatcaagca tttagaaaaa agaggtcttc tacatgatca
     1441 gcttgcagtt ggcggggttt cgatgggggg aatgacaaca tgcgccttgt tggctaaaca
     1501 tcctcatata gtagcgggcg tgtgtttgat gggttcccct gcacctttgg aatatggaaa
     1561 agaaatcgta aggcgcgcat cggaatataa ttatgtcgta ccaaaagact attttgattt
     1621 gatttcatgg gttaagagct atgatttgtc attgcagccg gaaaagttag ctggacgtcc
     1681 tctgttcttt tggcatggac aagctgacga aaaaatccct tatatacaaa cggaacgatt
     1741 tgtagaaaaa aatcggcata agtcttacgg aaaagaaatt gtttttcgtt cctcaaaaac
     1801 acagcgacat atggtacaaa taccactaat ggaagaagct tccacttttt tagcaaaaaa
     1861 tttgataaaa aagtataaat aattgaaacg gaaaatcact catgacgata gaggattttc
     1921 cgttttttat tttgggctat aataaactct gttaggaaaa tttggtataa ttgatagtaa
     1981 gaatgacaac atgttagaaa ggaagagagc atgcccactt tagcagatgt tgcaaaacgt
     2041 gccaatgttt caaaaatgac ggtctctcga gtcatcaatc atccggaaca ggtgactgac
     2101 gaattgaaag agttagtttt ctcagcaatg gctgaactgg attaccgtcc aaatattgct
     2161 gcaaaagcac ttgtcacgaa tcggtcgcag atcatcaaat tatttatact ggaagaaatc
     2221 gacacaactg agccctatta catgaatttg ctgatgggaa tcgctaaacg gatcggtaaa
     2281 gcgcattatt ctctccaatt agttacaaat gatgccttcg acgtcggttc ttgtgatgga
     2341 tacatcatca caggtgtcag aaaaaaagac ttcgattgga tcgaccgttt ggaaaaacca
     2401 gttgtcttgt ttggggaaaa ttctgaagga tatgattatg tagacagcga taatgaatat
     2461 ggtacagcaa aagcaacgca atatgcctta gaaagagatt atcaaacgat tatttatatc
     2521 ggtatcgaag tggacgagcc ttttgaaatt tcaagggaaa ctggttattt atctgtgatg
     2581 aaggaagttg atcttcctag taaaatcttg cggtttccaa atcattcgac agaagtagaa
     2641 agatatatga cagaaaactg ggcagatttt acagataaaa cttgttttgt atgtagttca
     2701 gatcggctag cactaggagc agtaagaggg attataaatg ctggaggaca cttgcccgaa
     2761 gaatttggtg tgattggatt tgatggggta tttttagacc aaatcagttc acctaaattg
     2821 acaacgatca agcaagatat tatacgtatg ggtgaagtat gtggagaaat gttgttaaaa
     2881 aaaatagaag aaaaaggggc gagtcaaggt tatcgtcgtt tttttcctga actagttatc
     2941 agagagacaa cgaattaagt cttattacta tatatatatt tatgaaattc atcgctattt
     3001 tcttaaaata atcagcgcat tgctactgaa aacaatgaat ctgtgctatt attgagaaaa
     3061 gttccatttt tgagaaggat ggtttgaatg aaagaaatca gacgaaaaca tttccgtttt
     3121 cctgcgtacg atgatgagtt aggcgtaaaa ttaaccaacg acaagaagcg agtattattt
     3181 gacggacaag aagatctttt gactgaacag aatcagtccc cgtttgaaca gatgaaagaa
     3241 tttcatttca cgaacacgaa caccgagaaa atccgtcgca agcgtcgtgc aacaaatgga
     3301 ttgaagtcag ctccgcgtaa ggaaaatttg gaaaagcata aaggaaaatt accggattat
     3361 accggccatt accaatcaga aatcacatcg acaggacgaa aaaaactttt tggtgattcc
     3421 tctcgttctg tcagacaaag tgaaacaagg acagagtcca agcatactac acgaaacgca
     3481 tctgttgaca cgaaggtgcg ggagcgttct tattttgttc ctaaatatgt acccgcatcg
     3541 attatcccag accctagaga aagcaaaatt tctgaggatc atttaatcga atcgatgaaa
     3601 aaaacggatg actcttatct gttgttcgat acagaaccga cgccgtatca agaaaaaaga
     3661 aatggtgagc cgtctgtacg aacattcaat cgaacaaaaa cgttagagga aacaaagcag
     3721 tcagaagaaa aaaaatcgaa aaaaagccat ggtatcttag aacgctcatt acaaggattg
     3781 atcgatgatc aaggaaactc tttgggagaa aatagctatt ttaaataagt gatatacaaa
     3841 tttatattcg gctacagttg gaaaggaaac gctgcggcgt ttccttattt tatactctat
     3901 ccgaaagttt atatcgtatc ctcaaatttt tttgagttta agcgaatttt ttctcccaat
     3961 aagtaagaaa ataatggcaa aataaaaaaa caaactattt tcgcagatcg tttgcaaaaa
     4021 tagtttattt ttttataaag aagattttac tgaataatat cattttagtt tacataatga
     4081 tatttctgta aattaaataa agtagtgaaa aataagaatc agtgctctaa ctgttcaaga
     4141 ggatcaagtg gttcaaatgt ttctgtagtc tgttttgctt ccaattgggg caaaacagat
     4201 ccaatttcca gatacttcac tttattcaat gtataagata gaatagaaca gctaagtaat
     4261 gtatacagaa aatgtgtgag atccaaataa agcaaggaga caactaaaat accaaaagta
     4321 atcaagaaaa gacttttgcc aatcttaata tgaaaatatt tttcaatggc ttgataaatg
     4381 atatctgttc cgcctgtgct tccgccgaat cgaaagacga gaccgcaacc aatgccagaa
     4441 agaatacctg tcatcaaact attgatcagt agactatcta cagcaaaatg tatatgaagg
     4501 ctctcccata aatgtaaaaa gaaaactagc gcaaatgttc cgacgatcgt ccggagaaaa
     4561 gcgtgttttc caaaaagaaa caatgagata agcaataaag gtaaattaaa aagcagtgaa
     4621 gtgacagaag gagcgaatcc aaaaagatta tgcaaaagca aagcaatacc agtaacccca
     4681 ccatctgcca gcttagatgg aatattgatt cctgtcacag ctaatccgta gattcctgcg
     4741 cctaaaatga ttccgcacaa ttctgtaaat aaatttaatt ttttcatttc tagcctcttt
     4801 tcttttgaaa attttcagca acttctttat tatactgact tatactctta cttcaagagg
     4861 aaaatgaaaa aaaattattt ttggctgttt ctttttattt atcaaataag gagtggaaaa
     4921 atgcgagcag cagacttatt gacaattctt acaaaatcac ccaaaaaaac agagtatcaa
     4981 ggagtttttt tggctcaaga acaaaaactg gtgcctgtga caatggtcca gataaataaa
     5041 gcaaatcaat ttatcttttt atttgaacac ggcaaagcac cacttgctat gaaagacatc
     5101 tttctttttt tgatgaagaa ccgtgagaaa gctgtttatt atcagtcaaa agaagaactc
     5161 attcctcttt atggagtgaa agagatagaa aataaagtgg tgatataagt aaaaataaga
     5221 aaacaagacg tttatgatgt caaaacaacc attttttaca tttttggcaa aaaagtgtca
     5281 tgatacagta taatattcgt tgaacaggcc aataaaggtg ttctccccca cgagatttct
     5341 actttattgg ctgtttcttt ttattaaaat aaaagttgtg tgtgtaaaaa agatatagct
     5401 gattccaagt gacaatttcg tatagatttt tatggtattc gtagtgaaaa aacgagaacc
     5461 aaaaagttga tccgactttg ttgcttggat tctttttttg tcagcatatt tagaggtatt
     5521 tttttctttt ttttgttcgt gctgtaaggt taaggcaaga caagttagtt gggaaaaagg
     5581 aaagaaatgt ttatacttaa agaataacag aaaggaaggt cgcatgaata attttataca
     5641 aacattaagt gaaagaaaag gcgagttgct ggaagctgca tttcaacatc tgtccatttc
     5701 tctcatttct ttgctgattg cagcactgat tgctattcct ttagcgattt gggcagccaa
     5761 tcataaaaaa atggcagaag tcttattgca gatcacgggt gtgctccaaa cgattccttc
     5821 acttgcctta ttaggtttac ttatcccatt tgtagggatc ggaactgttc cagcattgat
     5881 tgcacttgtt atttacgctt tgcttccgat tttccaaaac acgtatattg gtctagctga
     5941 aattgacccg tctattgaag aagcagcggt agcatttggt atgtccagaa tgagacgctt
     6001 gctgaaagta gagcttccca tcgcattacc tgtcattatc tcaggtatcc gaactgcctt
     6061 agttttaatc atcggtacgg ctactttggc agcattgatc ggagcaggtg gattagggac
     6121 atttattttg ttaggtattg accgaaatac acctgttttg acattgattg gtgcgatcag
     6181 ttctgccttg cttgcaatta tattcagcgg actgattcgc tggttgcagc ataagaaacc
     6241 tcgatattcc atcattactt tagcagcagt ggtgtgttta tttggcggcg tttctttata
     6301 tcagtctgcc ttgtttaaaa atgaaaccat cacgattgct ggaaaattag gagcggaacc
     6361 tgaaatcctg atccaaatgt ataaagaatt gattgaagac aacacaaaaa cagaagttga
     6421 attaaaaccg aattttggca agacgagttt tttgttcaat gcactggatc aaggtcaaat
     6481 cgatatctat cctgagttca caggtactgt cctagaaagc ttggtgaagg tacctgaagc
     6541 aatgaaaagt gctgatttga caaaagaaaa aacatatgac gaagcagaag aattattgaa
     6601 taagcaattc gatatgacat tattaaaacc aatggcttac caaaatacgt atgccttggc
     6661 agtcaagaga tcttttgcga acgaacatca acttaaaaag atttccgatt tggcagctat
     6721 ctctgatcag atcaaagcgg gattcacact ggaatttatt gatcgttcag atggatataa
     6781 aggaatccaa gaagcatatg gcttgaattt ttcaagtgta caaagcatgg agccaagttt
     6841 gcgttaccaa gcaatcaata atggagatgt caatattgtg gatgcctatt ccacagatag
     6901 ccagctcaag gagtacgatc tagtgactct agaagatgat cgagggttat tcccagctta
     6961 tcaaggcgca ccgctgatga aaaaagattt tgctaaagaa catcctgaaa tcgttaaagc
     7021 actgaataaa ttaagcggaa aaataacaga ggatcaaatg attgatatga attaccaagt
     7081 caatgtcgaa aagaaaaagc cgacagaagt agcacgaatc ttcctgaaaa atgaaggatt
     7141 agtaaaggag gaagatcagt gactaaatta atcgagttca aacatgtgca aaaaaagtat
     7201 gatggaaagt atgtcatcga tgacctaaat ctggcaatca aaaaaggaga aatctttgtt
     7261 ttggttggtc cttccggtag tggaaaaaca actactttga aaatgatcaa tggattatct
     7321 aaaccatcag caggagacat ctacttcaaa ggaaaatcgt taaatgaata taatctgcag
     7381 aaaatgcggt ggaatatggg atatgtgctg cagcaaatcg ctttgttccc gaccatgaca
     7441 gtcaagcaaa atattgaagt catccctgaa atgttaggtt gggaaaagca gaaaagagca
     7501 gaccgtgtag atgaacttct tcaaaaagtt ggcttatctc ctgatattta tcgagatcgt
     7561 atgcctagag agttgtctgg aggagaacag caacggatcg ggatcatccg agctattgct
     7621 gcaagcccag atgtgatttt gatggacgag ccatttagcg cattagatcc gatttcaaga
     7681 aactcattac aagaattggt tttgtcctta cacgaagaat tgggcacgac catcgttttc
     7741 gttacgcaca atatggaaga agcaatcaag ctaggtgatc gtattgcatt tatgaaggat
     7801 ggagaaatta tccaatgtga cacaccagag cagttattga tgaatcctaa aaacgactac
     7861 gttcggcatt tctttgatga accgaaacag acgaaagagt ggcgagtaga agatttagtc
     7921 gtcaatggat atttcttaaa tgaaatacca gaaaatgctc gtcaacaagt ttgctttgat
     7981 acgcctatga aagaagtata ttcgcttctt tcggtgtatc caagcattgt gatagtagaa
     8041 aagcaaagaa gtattgggtc gctgacaagt aaagaaatct ttgctttttt aagtcgagag
     8101 gaggaaggaa atgaaaacat atgatgcaat catcatcggt tcaggtgtga gcggcttaag
     8161 tgctgcttat ggattgaaag aagcaggtaa aacagtacta gtggtcgaag aagatctatg
     8221 gggcgggact tgtcctaacc gtggatgtga tcctaaaaaa gtcttgttaa gtgcagtaga
     8281 ggcacgtaat cgggtaaaac aattgagtgg aaaaggtttt aacgagatcc caactgccaa
     8341 ctgggaagaa cttcaaaaat tcaaacgaac atttacggat ccagtcccag aaagccgaaa
     8401 aaaacaatta gcagaagctg aaatcgatca tctttcagga accgctcgtt ttttggatga
     8461 ttcatctatt gaagtcaacg aagaagtctt ccatgcggat tacttagttt tggcaaccgg
     8521 gcaaagacct acgatcttac cagtggaggg aaaggaatac ttgaaaacta gtgcagactt
     8581 tttatcattg cctgtactgc ctaaagaaat catttttatc ggtggtggtt atattgcttt
     8641 tgagctagct acgattgcca atgctgcagg gagcaaagta accattgtcc atcataatca
     8701 acggccatta aaagaatttg aggcgtcttt ggtagaggaa gccgtccatc aaatggaagc
     8761 atctggcatt cagtttgctt ttggtgttga aacccaaaag atcatttcag aaggtactcg
     8821 ctatcgttta gtcggaaaag agacggaatt agtagcagat atgatttttt gtgcgactgg
     8881 gagacagccg aatacagaat ctttagcact tgagcaagcg aatatcgtct ttgataaaca
     8941 cggaattgca gtcaatgatt atctgcaaac gagcaatcca aaaatttttg cgtgtggaga
     9001 tattgtttca aggaaaaccc caaaattgac accagtcgcc acttttgaag gaaattatgt
     9061 agctaagcgg ataacggatg caacaagcga acctatcaaa tatccgatta tacctacgat
     9121 cgtctacgcc agtcctaaac ttgctgaagt aggagttaca aaaagtcacg cctcttcgtc
     9181 agatcaagtg gtagagatgg atcttaccag ttggttcacc tatcatcgtg tcaatgaacc
     9241 agttgctaag gctgaattga cctttgacca gcaaaattat ttgattggtg ctgcagtgat
     9301 tagcgagcaa gcagacgaat tgatcgatga tttgacactc gtcatcaatc aaaaattgac
     9361 taaaaaagag ttggatagct atatcatggg gtatccgaca ttggcaagtg atctctccta
     9421 tttattgaaa taatcagcat gataactaaa gcgcttgttg ctttttcctt cttgtgctac
     9481 actttaaaaa atgaatacct gtttctgggg atgtcgtaga cgacatgaga gtaagttgtg
     9541 aacttagacc cattgaacct gtcagttaat actggtgaag ggagaaacat agcagacgct
     9601 tgaagcgggt tactatgtcg tgcccatttc aagcgttttt tgttattcat attagaagaa
     9661 aggaggtaac aaaatgaaga aaaatcttta tccagcagtc agtatggcat tcattttaat
     9721 tatttggcag attgttgtca tagtttggaa aatacctgcc tttattttac ccggacctgc
     9781 tgctgtattt tctgcattag cggcagatgc acaagtatta ctttcccatt ctgtcacgac
     9841 attaaatgaa gcagtgcttg gtttggtaat agctagcgtt ctttctttta ttactgcctt
     9901 ggcaatggat tattggaagt ttttagaaat gtctttttat ccgttactag tgatttcaca
     9961 aatgttgcca atcatggtgt tagggccatt acttacccta tggtttggtt tcggtatgct
    10021 tccaaaagtg atccttgttg tcttgataag ctattttccg atcgtagttt ctttttcgga
    10081 tacattgagg aaagtatcaa aagagcaaat catctttttg aaaacgatgg gggcagatac
    10141 agggaagatt tatcgaatat ataaaatacc agcgggttta tccggcttct tttccggttt
    10201 aaaagtggct gctacttatt gtgtcagcgg agcagtcgtt ggagaatggc taagcgctca
    10261 agcaggctta gggtattaca tgatacgcgt aaaaaacagc taccagatcg ataaagtttt
    10321 tgctgcgatc atttgtgtca ttctattgag tttattgctg aatggcgctt gttcattatt
    10381 gaaaaaaggg tattatcgta tgctttatat tttttaagag aaggggatca aatgaaaaaa
    10441 ttatggcttt tattaccgct actgttgctt ttatccgctt gtggcactgc aaaagaaaca
    10501 agtagcaaac aagaaataaa ggatttgaaa aaagtgacac ttgttttgga ttatgtaccg
    10561 aatactaatc atactggcat ctacctagca aaagaaaaag gctattataa agaggctggt
    10621 ttaaatgtcc aaatcatcga accaggagat aattcgacaa gtattggtct agtaggtgcg
    10681 gacaaagcac aatttggtgt aagttatcaa gaagatgtca cttatgctca cgcagatgga
    10741 cagaatatac cagtcaaagc aattgctaca gtgatcaaac ataatacttc tggattcgcc
    10801 accctatcag acagtaacat ccattcacca aaagattttg aaggaaaaac atatgcaggt
    10861 tggcaaagtc caagtgaaga agctgtctta aaagctgtga tggaaaaaga cggcggggac
    10921 ttttcaaaac tgacaatggt aggaagcaat ggagaagggc cagaaagctt aggaaaatcc
    10981 tcagatatcc aatggtactt tgaaggttgg gatatgatca aagcaaaaga agcgggtatt
    11041 gaactaaact atatcccatt gaaagaatta gatgaacggt tagattacta tactccggta
    11101 attattacga atgatcaact gatcaaaagt gatccagaat tggtacagtc ttttatggat
    11161 gccacgaaaa aaggctatca ggaagctatt aaggatccaa atgacagtgc taaactgttg
    11221 caaaaatatg caaaagaaaa tgatcggacg tttttagaag aatcacaagc attcttgtca
    11281 aaaaattata cggatgatcc tgagaattgg ggattgatgg aagaaaaagt ttggcgcaat
    11341 tatacagctt ttatgcagga aaacggattg atcaagcaag atgtaccttc gcaagaacta
    11401 ttcactaatc agtttataaa ataggagtga tagatatgtc aatccaaatc agccacttga
    11461 atgtagtcat tgaaaacctg aaaatattag aagacatcca tttcactatc cctaaagata
    11521 cctttacatc gattgtcgcg ccaagcggag ccggtaagtc aacattgctg aaaactttaa
    11581 caggagtcca gcctatgact tctggaacga tcaaagtaga cgatcaaaaa gtaactggtc
    11641 tccatacagc atttagttat atgccgcaag aagatatgtt acttccttgg ctgagcgtct
    11701 atcaaaacgt tacgttatat caaaaaatca atcatctaac aatcaatgaa gaacaagtaa
    11761 gggattatct ggatatcttt gggttaaacg gctatgaaca ttttttgccg gaacaattat
    11821 ctggcgggat gaagcagcga acggccttgt tacgaacaat catgaatcct tcttcttact
    11881 tgcttcttga tgaaccattt ggagcgttag atgctatgac cagagggcag atgcaagact
    11941 ggatgttgaa gctgcctaaa aaagcaaagc ggactacttt acttgtgacg catgatatcg
    12001 aagaagcaat ttatttatca gacaggattc ttgtgctttc cgcccgtccg gcacatgtga
    12061 ttgctgagat tcaagtgcct gaaaaacgac ggtcacgtga atggctactg cagcaatcag
    12121 agttgaagca aacgatttat cagttattgg caggtgaatt agatgttaag tgatgctcat
    12181 tgtcatttag atgggagtca gagtttagca ctccttcagc aggaacacac gattttgacg
    12241 atcatcaatt gtgattcgcc agaagaatgg aaggaaaaca gacagcttgc agcaagtaag
    12301 actcaagcct taagttacgg tatccatcct tggaaagcag atagctatac gtttgaacaa
    12361 gttgagcctt ttttgaaaaa agcaaggatc atcggtgaaa tagggttaga taatatttgg
    12421 acgaatgtac cgatgactac acaaaaaaaa gtatttgagc gacagcttgc ttttgcagcc
    12481 ataaatgaga aacctgttgt actgcacaca aaaggatgcg aaaaagagat cttaaattat
    12541 atccaagctt accctaatca ctatttgatc cattggtatt cttctttgga ctatcaacaa
    12601 gactatattg atttaggctg ttatttcagc attggtcttg atttgaaaaa gaatccagca
    12661 gtctggcaac tagctaaagc agtcccaatc gaccacttac ttattgaaac ggatggacta
    12721 catgcagcag agtgggcttt aggggggatt ttttcagaaa aagattatgt ctcactgctt
    12781 gaagaacagt taaagacaat cgctgagatc aaagagctat cagttgaaga agttaaaaaa
    12841 aatacttatc aaaacttgga acgtttcatt agattaggta aataaaaaag agatgtgaca
    12901 caactagtgt cgcacctctt tttggctttc tttgtataaa agtaaactgt ttgattaata
    12961 aatgaatctt agtaccaacc gtttgcttcc cagaatgctt tcgccgcttc ccaagaacca
    13021 taacgtgaag taacatattg ttctgctact ctttcttggt ttgcagcaga gtagtcacca
    13081 tttaagtaag aagaatctaa ttggtaacgt ccgatgtaac gtccgttcgt tgctgtgtaa
    13141 gaaccgcttg attctttttg tgcgatccat tcttttgcag agcttgtgct tgctggagta
    13201 gcagcagttt ctgttacagc aggagcaggt tgtgtttcag caacaggcgc tgcttgtttt
    13261 tccgtttgaa ccgtttcttg tacaacaggt tgttcagctt ggactggtgc ttcttgaacg
    13321 gctgcttgag gttcagcagc tgcttgtgca gagccttctg ttgggattgt taattgttgt
    13381 cctgagtaaa tcaggttaat atcagaaatt
//
LOCUS       DACCXO010000074        13338 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.075, whole genome shotgun sequence.
ACCESSION   DACCXO010000074 DACCXO010000000
VERSION     DACCXO010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.075"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            948..1421
                     /locus_tag="IVY70_001935"
     CDS             948..1421
                     /locus_tag="IVY70_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP7959059.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1430..1657
                     /locus_tag="IVY70_001936"
     CDS             1430..1657
                     /locus_tag="IVY70_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7959060.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2293..2664
                     /locus_tag="IVY70_001937"
     CDS             2293..2664
                     /locus_tag="IVY70_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959061.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2920..3162)
                     /locus_tag="IVY70_001938"
     CDS             complement(2920..3162)
                     /locus_tag="IVY70_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7959062.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3194..4096)
                     /locus_tag="IVY70_001939"
     CDS             complement(3194..4096)
                     /locus_tag="IVY70_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7959063.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4109..4297)
                     /locus_tag="IVY70_001940"
     CDS             complement(4109..4297)
                     /locus_tag="IVY70_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP7959064.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4311..4874)
                     /gene="amaP"
                     /locus_tag="IVY70_001941"
     CDS             complement(4311..4874)
                     /gene="amaP"
                     /locus_tag="IVY70_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP7959065.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4912..5805)
                     /locus_tag="IVY70_001942"
     CDS             complement(4912..5805)
                     /locus_tag="IVY70_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7959066.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5883..6821)
                     /locus_tag="IVY70_001943"
     CDS             complement(5883..6821)
                     /locus_tag="IVY70_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7959067.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6855..7205)
                     /locus_tag="IVY70_001944"
     CDS             complement(6855..7205)
                     /locus_tag="IVY70_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP7959068.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7238..8140)
                     /locus_tag="IVY70_001945"
     CDS             complement(7238..8140)
                     /locus_tag="IVY70_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP7959069.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8133..8990)
                     /locus_tag="IVY70_001946"
     CDS             complement(8133..8990)
                     /locus_tag="IVY70_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP7959070.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9324..10133
                     /locus_tag="IVY70_001947"
     CDS             9324..10133
                     /locus_tag="IVY70_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7959071.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10173..10670
                     /locus_tag="IVY70_001948"
     CDS             10173..10670
                     /locus_tag="IVY70_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7959072.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11055..11128)
                     /locus_tag="IVY70_001949"
     tRNA            complement(11055..11128)
                     /locus_tag="IVY70_001949"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11092..11094),aa:Ile,seq:cat)
     gene            11315..11638
                     /locus_tag="IVY70_001950"
     CDS             11315..11638
                     /locus_tag="IVY70_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959073.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11802..12056
                     /locus_tag="IVY70_001951"
     CDS             11802..12056
                     /locus_tag="IVY70_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959074.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12126..12371
                     /locus_tag="IVY70_001952"
     CDS             12126..12371
                     /locus_tag="IVY70_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7959075.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12447..12812
                     /locus_tag="IVY70_001953"
     CDS             12447..12812
                     /locus_tag="IVY70_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959076.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
BASE COUNT         3883 a         2467 c         2239 g         4749 t
ORIGIN      
        1 tttcggagaa ttaaaaaata caataaataa ctttagttac tttaatgcgt tcttacttta
       61 tttttatatt atcatttttc ttattcactt ctttggttta tactattctt ataaataaaa
      121 acgaggtatg tcctatgatg aaaagattgt actattcatt gatcataacg attggctatt
      181 tgattgtatc aaatctagga aatatggtat ttggtatcag taaagaattc agttggacta
      241 ctacattatg ggaatctcta ttctttttca tcttcgtatt tttgttacaa aattatcgaa
      301 aaaaataaac atgtgccatc tatctgttgg cacatgtttt aggatttatg gtacttttta
      361 ttcggcaatc cattttattt ttctacttct tcttaatacc tctaacactt ttgatttata
      421 aactaatcca atgcatctac ttgcgcttat taagctattt cagctagata gtaccacccc
      481 atgcgtcaat gcttgatttt ccttttgtat aacattgtat aagcaataaa ggacaaataa
      541 gtggtgatta caccaaacca ttgaataaga aaaattctga tacgtaaata aacatgaact
      601 tgaaaaaatt aatgaatcgg ttagctacta cgtagttgtc ttttttctca tacagttctg
      661 ttaacttaca cttctcttcg ctagataata aaaaatcctc caactgtatt gtccaatata
      721 atcccaggca tctaaactag cattatagaa gagacacaca tagaatctaa aataactggt
      781 tttaaatgtt tcttacaatc aaaaaaagct gttaaatgca ctccacacct tcatactcta
      841 tcatttttta taaacgatat atttttattg tttttcgata aaaaatagtt gttttacaat
      901 aattattctg ctatatttta gctatcaaag ataaaagagg gataaaaatg aaatttaaaa
      961 cattgctatt aaaatttgtc gttttaggta taagtgccgt atttattttg ttcggcgtac
     1021 ttttttatac tcagatcgct acaagcgaaa aaggattcaa ttttgattgg cagaccacat
     1081 tatttgtttc tgtcatttac tttgcccttc tcctagggat cgttattgct tatttcttat
     1141 tgaaactatt gaaaaaaatc gaagataacc aagctttttc cactcaaagc ttaaacatac
     1201 taaaaaaaat cgaacgaacc attttgttta ttggtttcgc cttttttggc ttacttccta
     1261 aatttatgaa agtagcggat ttagacgatg ctccaggttt gatgttggta ggaatcgctg
     1321 tcgtattcat tccttttgca gtatacacat taacaagcgt gctggaaaaa ctgcttgaac
     1381 atgcgatttt acttaagaca gatcatgatt tgacagtgtg aggtgcgaca tggcaattat
     1441 cgttaattta gatgtagtat tagctcaaaa aaaaatgagt gtgactgaat tgagtgagaa
     1501 agtgggcatt acgatggcaa acatctctat tctaaaaaat ggaaaagcaa aagccatccg
     1561 tttttccact ctcgaaaaaa tctgccaagt tttggagtgt caaccaggtg atttgttgac
     1621 ctataaaaat gactcgaata ctataccaga agaataaaat aaagtagaac aaacagtaaa
     1681 taaaaaataa tctgaatgat agatgtgtga gacttttaga agttatttac gtctattatt
     1741 cagatttttt atttcgattg aataccgttt tatctataaa taagatatgt actgactcat
     1801 acaaaggggg ccttttcttc tccatgtact atcacttttc ctgatacaac tggtttcctg
     1861 ttacgttgta gttggatctg cttttagtat aatactttat tcattccacc agactgatag
     1921 cagtttgagc agtctaaata atcatgtagt attacaggcc attctgtttc ataccttgaa
     1981 gtgcttaaaa cttcagctaa tggcccattg tgttaactct tactccattc ttttttagta
     2041 agctcccata tatactgtaa agccttctat atattatgtc atgttgcttt ttaacattta
     2101 ctatgtagtt ttgataaaat gatttgaata acagtgatgg acaattcttt tttattttta
     2161 acattatttt gataataatg attatttatt taaaaataca ttagataaca tttgttacga
     2221 cacgaaaaag cttcacaaac aaatatcgtt attttaaaat gagggcgaac tcacacaatt
     2281 aggaggaata gtatgccatt tattgtttac ttttttcttt cattactcgt gatctatatt
     2341 ccattaccaa catttttcct gctcgtcaat cgtctagcag agcaacatga tacgattgct
     2401 gctcttacga aactttgttt acttctattt gtttgtctag attactttac gagctattat
     2461 tggctaaaac agacaagagt aaaaaagaga tattatttct tccttgtctt tttcaatctc
     2521 tttgagtttc tgatccattg ctttttactt catcagtatc atacgatcca tttgtggatc
     2581 atctgggttt tcaaagtggt cgtattattt tgtatgttca cctttcctat ctcaagaaaa
     2641 tttagggagg cattgtttta ttaaagaagc ttctctgtat caataaagac acatttaagg
     2701 aaaaactttt aaaaatctaa ttgggaattt gcccgcataa ataagaggct gtgacaaaag
     2761 tcttgtcaca gcctcttatt ccgtataaac agtgttcaaa agcttgcatc ttcgataata
     2821 tgcccaacca aatgaaaaat atgagaagct atttttgttt ggctgttctt attacttgat
     2881 gaagaacagc tttttcacga cctcttttta aaagtctttt tacagttttc gagcaaagaa
     2941 tgatacgatt agtactaaaa tgatggcacc aatcaatgat ggcacgattg ccattcctgc
     3001 aatacttggt ccccaagaac ctaataatgc ttgaccaagt gctgatccaa ctaaacctgc
     3061 tacgacattt gcaataatcc ccatcgaaga acctttattc gtgattgctc ctgcaattgc
     3121 tccgataatt cctccgacga taagtgacca aataaatccc atgtttcgtt cctcctaatg
     3181 ttttacttcc ttattactga acccgtggtt ctgtattttc ttttactttt tgtgcgccgc
     3241 ttccagctgc tgattttact ttatctgttt gattcgaagc aaattcaccg gtttttttcg
     3301 ctgcgtcagt tactttgtct tgcaccgttt cttgatcttt ttcaaattct tcttttgttt
     3361 tgatgtccac tacgttagcg ttgacttcga tcacttctaa atgtgtcata ttttttactt
     3421 ctttgccgat tacttcttgc atttcttcaa agattttttc gatatctttg ccatattcca
     3481 caatgacatc catatcaact gctacttgtt ttttaccgac ttctgtttcg atcccagccg
     3541 ttacattatc tgtattcaca agttttcctg ccacattaga gaagaaacca ccgtttgcag
     3601 ataataaacc gtctacttgt tcaattgcga ttccgatgat tttttgcaca actttatctt
     3661 cataattcag ctcaccgcgc attgaatctt cctgttcaga atcatgcttt ccttggttat
     3721 ttgtatgatc agaaccaagt tttccttgtc cgtttgtgtg gtcagaacta agtttttctt
     3781 gtgctttcgc atggtcagaa ggatgttttt gctcttccac aggtttagaa ggatcgattg
     3841 catcatgttg ttctggaact ttagattctg gagttcctgg atgatgaggt gcatgctttt
     3901 ctgcatcatg tttcttctct tctactcctt gttgaacgtt agggactact ggatctggat
     3961 gtgcagatac tggtgctgtt ttgttctttt catcgttcac accatttgat tggtttggtg
     4021 tcgttgggtc tggatgtgca gacactggtt tttggttgtt cattttattg ttattatttt
     4081 tgttattgtt attcatggat gtttcctcct aagatttaaa atagtgatct aagattttcg
     4141 tttgtttcag ataaaaaccg atagcgacgc cgatcgctgc catgatcaag atcaataagg
     4201 tcttgaaaaa gcccacggtg atccataaaa cggctaatat caatccaagt accccaaaga
     4261 tgatagggag cttgtatgtc gaaaatagtt ctttcatttt tggtcccccc ctattctact
     4321 cgtttgcggt cattgcttcg tttagctggt tcttcaaagc ctgtgtattt caccgatact
     4381 ttcactggct cttttgttcc caatatgtgt tgtacttcat tttcgatttc tttcatcaat
     4441 gttcctgttt taccaatgag tgctgaagtt cgtttcaact ccccttttac attgacggcg
     4501 atcttgtttt tcgttgcttt gacatttact tttggtgaat ggatgaattc atcttcatgc
     4561 aagtgagaac gaaccattcc ttcaattgct tttttatcca acgtcaactc cccatcacca
     4621 tgtttcagta gaaagtttcc gattgttttt ggataaaaca ggaaaacaag taacgtgata
     4681 attgtcaaaa tagcaaatgc aacggcaagc caaaatacat attgttgcac gtatgaatta
     4741 gtgatggaaa agaatcgaaa cttctctagc tgaaaaggca atggataggt tgcacttaat
     4801 agtatcgcaa acaaaagcac agatagcaaa agtaatgttc cgatcactcc gatcgttttt
     4861 acacctttat tcattttcgc acctcctcat aggggtgaaa cgatcaactt cttataagtt
     4921 gatcgcttct ccaccagtta ctccgtaaat ttgggctgta acataacttg ctttattcga
     4981 tgctaagaaa acataaactg gtgctaattc tcctggctga cctgcacgtt ctagtaaact
     5041 ttgctgacca aattcaggga tcgaatcaga aggttgtcct tcgtctagtt gtaatggtgt
     5101 ccagattgga ccaggtgcta caccatttac acggattcct ttaggtgcaa gctgttttgc
     5161 taaaccgatc gtaaagttag cgattgaagc ttttgttgct gcgtaatcta ataaatgttc
     5221 gcttggattg aatgcttgaa cagaagttgt tgttacgatt gctcctcctg ctgggatatg
     5281 cggaacagct tctttcacta aagcaaacat agcgatgatg tttatcataa atgtgtcttt
     5341 gacttgctca agtggtaact cagcaataga ctgacaagaa atttgctgtc ctgcgtttag
     5401 taccatcgta tctagcccgc caagttcact cacggcttta tctatgattt ctttaggtgc
     5461 ttgttcgtca cgaagatcaa aaggtagtaa gacaatctta cgtcctgcaa ctttcacgta
     5521 ttttgctact tcctctgcat ctttttcttc tcctgggaag aattgaagag cgatatctgc
     5581 cccttctcta gcaaatgcaa tcgctgctgc acgaccgatc cctgaatctc caccagtgat
     5641 tagtacacgg cggttttcta gttgattatt gccttcatag gattcttctc cacaatcagg
     5701 aacaggaatc atattctgct gtaaagctgg tgtatcctga tcttgtttag gaaatttctc
     5761 cgtatggtac aagtttcgcg ggtcgattaa ttttggttct gtcattgctg tattcctcct
     5821 agtttttaaa ttgtattgcc aaaacaaatt tctttgtttg atacctgttt taaatagatt
     5881 ctttaatttg tataatcttt ccggtataaa tggtaaccta aaactactgc tgctatcaaa
     5941 gagccaaaga ctacgagtat aaagcctaga gcagaacttt tacccggtaa tcctcctgta
     6001 ttcattcccc aaatccctgc gaacaacgcc ggaacggaaa tgattaatgc agctgaatct
     6061 agatatttca tgatatgatt tagattattg tccatcatcc cgttgaatag atcaccaata
     6121 ctctctaata aatctcgata gatctcgatc attttttctg tttgtcgctg tctcaaatgg
     6181 acatcataga gtagctgttc atcgtctaaa cgtttgatga aagtttcaga attccataat
     6241 tgatccattg tttcccgttg atctcttaag gtatgatcga tatatacgat gtctctttca
     6301 agatctgcct gtttatacag ttcttcattt tccgttgttt tacgagctgc tttatccaaa
     6361 gcgtcaattc gctttttcat ctctcttagt tctttcacgt aatgggtgta aatatctaat
     6421 aatgtatacg tgatgatctt ttcaaacgag tttaaatgat ttccatgctt tgtcagcagc
     6481 cgatccacaa attcggtttt tctatcgata caagtaatca agagattatc ggaaagtaca
     6541 aaagatattg gcgttaattt tttttcgata cgattatctg actgatctag attaatcact
     6601 accaaagaaa atggatgctc tagtttcgtc tcataaaggc gctccatccg agaaacttct
     6661 tccggatatt cggttcctac gaaaatatca tcaggaagct cgaattcttc gattgcttgc
     6721 tcaatctctt cgggctttgc actatcaagt acgagccaat tatatttttt ttcatctgtt
     6781 tctattacat tcttttcttc atccacaaag tagtatctca atgtatacct cctttttgga
     6841 ctttctcgaa actattagct gaagaagcag ttctttaatt tgatattata atcgagtact
     6901 gcgatttcat tttctaatga aaagctgtca agctgtttgt tgaagtccat gttcaatcgt
     6961 ttaccgaaat aattttgctc taatttagat gtatagatgt ccaataatgg atgctcgatt
     7021 ttagtcgcat attctccaaa ttccgaaaca aaaggaatga tttgaaaaaa gctgcctttt
     7081 gcaaaagcac ctttgtccct tagaaatgga tcggaatcat tgataagcgc gagtgcgatt
     7141 tttgtatttt ccccaacctt ttcaattaac gtctctactg catttttaga cattgtaatt
     7201 tccattccta ctccccctct tcagttgttg tcttctatta tttaacaact acatctgttt
     7261 ttggttgaac ttttgcatat tttggatatt tagggaattt agctacacaa cggaaagccg
     7321 tcatccctgc atatttttcg taatatttct tcgttggccc ttcaaaatcc ttgctccacg
     7381 gtttcacatg tcctgaaaaa tggataatgt aaggattgtt gcgagtttct tcgtattcac
     7441 gttcacctgc tgctgttgga tgtttttttg cttttgccat gatatagcct tgtgcattcc
     7501 aacgcggatg aagtggtaac caacggtcat gtaagatagc atttaatgca tcttggtcat
     7561 ggaatcgtaa tttttctgga ttatgttcga tgaaatctaa tactttttgc gtaatatttt
     7621 catctaacca ttttttcaca ttgatcagca tcaaacctga gttgaaataa cgcattgatt
     7681 tagctgaaat tttcattttt tccagtcgtt gatggaaccc agcgtcttca acagctgcta
     7741 cgattgaatc accgaagtca agacgccata atttagagat atcttgtaaa gcgatcatgt
     7801 cgcaatccat atataagata cgttcaactt ctgtgccgcg gaacaattcc ggaatcgcaa
     7861 tacgataata agctgtttct ggaatacgat cgctgattac cacatttgta aagaattctt
     7921 tattgatttt caagaattcc acatctgagt tcattcgtgc gtttttcact gagaagcgta
     7981 aaagctgttt actttcaaaa tcgatatcat cgtcaatcac atagaaccga acaaatttag
     8041 acgggttgca attatctaat actgagacaa ataatgcagc taaatgtggt acgaattttg
     8101 tgttacaact tgcgactact gctatttctt ttttattcat tgatctcgcc tactttcgtt
     8161 agagtactgt gtgctaattt ttttaagtat aaattggtat acggatgatc tttcaacgta
     8221 ttccaaggtt tatcatgtcc tgtaaagtga acgatcgaag gtttttcatt tccttcttta
     8281 tactgacgtt catatttttc atttggtgct ggatgacgtt caaaaatcag cgaagtctgc
     8341 atgttccatt ttggatgaag ttgttcccaa tcttcataaa gtacagcatt caaagcatct
     8401 tggtcgtgat agatgattcg atcgccattt tcactgaggt aatggatagt tttttctgta
     8461 atttcttttt tattccattg atcaatgtca atcaccatca ctcctgaatt gaagtacaag
     8521 tcatcggaat cgattcctaa tctttccaaa gctttcactt ggcctggatc gataatagcg
     8581 ccgattgttt ttccatttaa cggctcatca tatagtttta cgatatcgtc tagtactaac
     8641 acatctgaat ctaagtaaag gactttctta taatcctctt tagctaataa gtttggtaaa
     8701 gaaattctta agtaagcggt agttgtgata tggtcactga ctaggaagtc ttcgtagata
     8761 tctttttcca ctgtcaggaa ttggatactt gcattacttg aatatttatt taccgtttct
     8821 tccaatcctt ctttactata ttctgataga ccatcgtcaa tcacataaaa ttttatgcgt
     8881 cttgttttat tgcaattttc taatgcagtt gcgatcatca cgctcaagta aggagcataa
     8941 ttttcgtctg aagcagttac cacaggaact acgccgtatc tcttctccat caaaatcact
     9001 cctttttcta atctttttct aacctgttct acatctagaa tattcctcat ttggttttaa
     9061 agcaaaatat atgctttgtt ttcattgaat attttaaaat ttttttatca caaaacgcat
     9121 cggtaagcgc taactattcg tttttcatgg ctttttattc aaaggtattg accaaaaact
     9181 actatatata gccacctttt tcttaagaat gttttttgaa aaaattcata atgacaaagc
     9241 aagttagttt cattttttta aattctcatt taatcttaag gtaacaggaa aatagatatg
     9301 taaaaagaaa gtaggaagat aaaatgaaac tagcagcgat tgatttagac ggtaccttgc
     9361 ttgatcgaaa tggaaagata ccagaagaaa atatccaagc acttcggaaa tttgatgaaa
     9421 atggcggaat cgtaaccgtc gctaccggaa ggaacagcat ttctgctaaa gatatttttt
     9481 ccgaattagg aatcggtggc tatttgattt cttccaatgg ttcactgatt gctgaaatga
     9541 aggatggaaa aatggcgaaa gttctcagac gttcaaaaat agatagcgat acactgagaa
     9601 aagccttctt ctatgctcaa gaagcgaaaa tatccatcat tgctagccga gaaacccaag
     9661 atgatcagct gacattcgat gaatcaaatc tagtcaaaga tgacccttat tatgaacatt
     9721 tcaatttaca gaaccattct tttgatcaaa ttttaaatat gttggaagat ccttctctta
     9781 gctatcttaa attagcatta acagataaga gtgaagaaaa actacagaag ctacaaagag
     9841 atctgcaagc agatggtatc caatcagtat tctctgatcc gcatttctta gaaatcacgc
     9901 ctacaggtat cactaaagct cattcacttg tttttctggc ggattacctt ggaatcgaat
     9961 cgagtgacat catggctttt ggcgatcaag aaaacgacat tgctatgttg aattacagcg
    10021 ggttgagtat tgcgatgggg aatgcacagc gacaagtaaa agaaattgca aaccaagtaa
    10081 cactgacgaa tgaagaagct ggtgttgctt atattttaaa aaacttctat taaaaagaat
    10141 aaaatgacga tatgaatgta ggaggaaaga aaatgttagt tattattgat atgcaaaatc
    10201 atattcttga tccaaacagc gaattttata ttccagagtc agatcaattg gcagaacgta
    10261 ttgctcaaag attagctaaa gcacgccaaa ataatgaata tattctcttc acacgggata
    10321 taccagttga actgaaggac aaagaagaag aacgagaaga tttgcaattg atccctcttc
    10381 tttctccaca agaaaatgaa cgagtgatta aaaagtatta ctttacgatt ccacctgaaa
    10441 ctttagctga aatccaggaa actttatttg aaagcaaaga agaacaaaaa gagatcgaag
    10501 tagtcgggat tgaaacaaac ttgtgtgtac tctctaatct gataggcctg cagagtgctt
    10561 ttcctgaagc agactttttt gttgatccaa tgttagtctc cagtagaaaa cacggagaat
    10621 ccgctttaga gctgttgaaa gatttcaacg tctctatcgt cgaatcataa aaatatctat
    10681 cttcatttac gatatttaac acacaataaa aaaagcacgc agaactgttt tagtcctacg
    10741 tgctttttct atcaaagtag attggctttt ttatgacaca gagtgaaacg gcaggctcac
    10801 gacctctttc ttaaatggtg ttcaatcagc tgaccctcgg tattagctta acaaacggca
    10861 aaatatgagg aactattttt ccgtttgtta agctaatact caggtcaaaa cgctgatttc
    10921 acaacctctt ccttaaacgg tattcaaaag cttgcatctg cagtaataag aacagccaag
    10981 taaaaacata agaagctgtt tttacttggc tgttcttatt acttgatgca gggcagcttt
    11041 ttcacaacct cttatgtacc ttgtaggact cgaacctacg accggacggt tatgagccgt
    11101 ctgctctaac caactgagct aaaggtacat cataaataaa atttattttt gttacttttc
    11161 aactatagca cgaatgacaa actctcacaa gtttcaaaat ataagtaaaa taatattgtt
    11221 attcttattt cttttacttg tgtcataact attttgttac tttaattttt atttgcttat
    11281 tttagaaaaa atgttattct tttatttatt ctcaatgtta ggagtgatta ttttgccatt
    11341 tattgcgatt ttattcgatt tagttgcggc agcagcttat tttctccaat taaattatca
    11401 aacctcagga attctgttta ttggaatgat ttttcaaggt gtgattaccc ttttgcttct
    11461 tgtcatgatg ctcagctata aaggaaaaag atatgcccgt attcaaacaa aagtatttgt
    11521 taaatatgtt tccattcgct atgggatcat cattctttcc tttttagtga atgcgatcgt
    11581 cctcttttta tatgtattga attacttaga tatcaatcca ttgatttttt ctcgatagat
    11641 aagacacatt cagcggaaca gttcctgctt atcagcagaa aactgttctg ctttttcatt
    11701 tttcattgat ctattcttct tttattttcc tagcatttca tttgtgatgt atgcaataac
    11761 atgttatctt ttattatatg aatcgttgag gagtccgtta catgattacg ttggtgagag
    11821 ttttgttttg ggtgcctgcg gtcgcactgg tcgcaagtat tgtttatctt atgaattgga
    11881 acaaagaacg gttttattta gctattttga ctcttccggc tatctacttt atgtggaaag
    11941 tttttaatta caattacttt gaacccgata gtgtttttat agaagaatta tcaggactgg
    12001 ttttgtcttt attgatcgtc attctttatt tgatacgttt aaataaaaaa cattgagtac
    12061 tattgattgc atgtgtagct ttctatagtc attataacta ttctactata atgaaaggtg
    12121 attaaatgag tatcgaagaa gctattttac tggatacttt acgttgtcgt ctgaatttct
    12181 taagaactgg ccgatcttta tctcaagtag ctgtttctag cgcgttaagt attcctttat
    12241 tcaaatatcg taagtacgaa gaaggagaag aaattccaag catcattgaa ttaatctata
    12301 tcgctgactt ttacgatatt tccgttgatt acctaattgg tcgctcagaa acagcaagca
    12361 aactgaagtg attttttcca gccttggtgc tggttttttc atttaacgtc taaggttaac
    12421 aatctatata taaaggagac atttctatgc gattatggca tgaaaaattg atttcaaagc
    12481 ttcctcgtca acagctttta ggacaacatc gggaatgctg tgcattaaga ggaaatggct
    12541 ggggaaagaa acatgctact gtggattatg tttttcacta ttctccttat agattattcc
    12601 aatatcatca gcttgtcatg gatgaaatgg aaaaacgtgg ttacagccca gcaccagagt
    12661 ggaaaaatcc tatgtatcgt ggcaaatcat gcgaagcatt ttttgattta cctactgtag
    12721 agcgcaacgt gccgatttat ccggaacacg atgaagttta tctagcagaa tgtttagcaa
    12781 atttggagca aaaaggaatt tatcttacct aatataacta gaaaagcctt ctatctcctc
    12841 ttacttcatt aaaaagcttt aaccatctta gatcaagcta aaatgtcgta agttttaggg
    12901 ttatcttcat cctcacgtgc aaaactaaaa gcgtactcta gttttggttc atcgccaact
    12961 tccctagaca taaatgaaca tctcctgacg tggtttaaaa gtaccgcatc ttcgacgtct
    13021 gtatacgatg cacagcacag attgcttctt ttgatctttc ttttgatttt tctgttgctt
    13081 cttctacacc ttcttcgatt actacatcga tgcttacaat tgcttctgcc taatattccc
    13141 atgcatcgtc gatttaagcc tctgtagtca ctgtgatcgt ttccacattt taactcctct
    13201 gtcgtggctt cagttgattt tgtgatcagt tcagttgcta agccagagtc agaacaacct
    13261 gccattgcta gcgttaatat tgatagtaaa aatagttttt ttatcataag gttccccccc
    13321 tgaaaataga ctaaaata
//
LOCUS       DACCXO010000075        13264 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.076, whole genome shotgun sequence.
ACCESSION   DACCXO010000075 DACCXO010000000
VERSION     DACCXO010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.076"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            4..2376
                     /locus_tag="IVY70_001954"
     CDS             4..2376
                     /locus_tag="IVY70_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959077.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="IVY70_001955"
     CDS             2437..3897
                     /locus_tag="IVY70_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP7959078.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /gene="topB"
                     /locus_tag="IVY70_001956"
     CDS             3908..6112
                     /gene="topB"
                     /locus_tag="IVY70_001956"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7959079.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6353..6946
                     /locus_tag="IVY70_001957"
     CDS             6353..6946
                     /locus_tag="IVY70_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP7959080.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6959..7858
                     /locus_tag="IVY70_001958"
     CDS             6959..7858
                     /locus_tag="IVY70_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP7959081.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7861..8358
                     /gene="ssb"
                     /locus_tag="IVY70_001959"
     CDS             7861..8358
                     /gene="ssb"
                     /locus_tag="IVY70_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP7959082.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8724..8984
                     /locus_tag="IVY70_001960"
     CDS             8724..8984
                     /locus_tag="IVY70_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959083.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9429..9635
                     /locus_tag="IVY70_001961"
     CDS             9429..9635
                     /locus_tag="IVY70_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP7959084.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9683..9886
                     /locus_tag="IVY70_001962"
     CDS             9683..9886
                     /locus_tag="IVY70_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959085.1"
                     /translation="MVDKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFEDLVKIAG
                     YELHYQENMETYENEYWLTKLEN"
     gene            9902..10339
                     /locus_tag="IVY70_001963"
     CDS             9902..10339
                     /locus_tag="IVY70_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959086.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10332..11039
                     /locus_tag="IVY70_001964"
     CDS             10332..11039
                     /locus_tag="IVY70_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959087.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11419..11598
                     /locus_tag="IVY70_001965"
     CDS             11419..11598
                     /locus_tag="IVY70_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959088.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11923..12186
                     /locus_tag="IVY70_001966"
     CDS             11923..12186
                     /locus_tag="IVY70_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP7959089.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12180..12530
                     /locus_tag="IVY70_001967"
     CDS             12180..12530
                     /locus_tag="IVY70_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7959090.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12554..13168)
                     /locus_tag="IVY70_001968"
     CDS             complement(12554..13168)
                     /locus_tag="IVY70_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7959091.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT         4904 a         2194 c         2730 g         3436 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaaa
      121 atgacgaacg gtcaaccaat gaacgacaag aggaatggcc gttcatggtt taaccagcaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaagaatac
      241 gtggaaaagc ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcgg ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctaga
      661 ccaactgatt ctatgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt cgaattgatc
      781 gatcaactca aaacattgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaggaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaaccaat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaagggt tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctatacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tttagaaacg ccagagaata agaatattgt gcccgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagatta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgaaacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaata tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaaag agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagataaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagtcgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aaatcattta tgaagaagtc gcaaaagaag aaaaggtcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaact ggtttccgct
     3541 cacaaagtaa atgcgttttt tggccagcag ctttctgtta acgattcaat agaagccatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagtttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc gcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg agatccagca acaagaaatc
     3841 agaaagcaaa aacgtgccat gttgtttcaa caaggcagac aacaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca caagcgaaag cttatgccga
     3961 tagcttctct aaagccactc gaaaagacgg gtattttgaa atccaagatc gattgtttcc
     4021 tggtgaaact gtgatcacgt atggttttgg tcatttagtc gaattagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agaacatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatcgcttg
     4261 gacgatcatt caacaagtag gcgccgatca aggaaaaacc tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagacg caaaagagac
     4381 ctatccaaaa tttgaagaag cacaagcacg tcagatcgcc gattggttaa ttgggatgaa
     4441 cggtagccca ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gcttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttgcaggaaa agattagaaa
     4561 tttcaagaaa gaaccttatt ttgaaggaaa atccgaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggcatg attcgccaag ttgaaaccga
     4741 gaaaaaaagc gcggcaagtc cacgtctttt ttctctatcc agccttcagt ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagacac tttggaagcc atgcaaggct tgtatgaagg
     4861 gaaatatcta agctatccgc gaacggatac cccttacatt acggaagggg aatatgcgta
     4921 tctgttggat cacttagacg aatacaagca ttttttgagg gctgaagaaa tttcgactcc
     4981 tattcataca ccgaatagta ggtacgtgaa caacaagaaa gtccaggaac attacgcaat
     5041 cattcctaca aaaacagtta tgacagaagc gacgtatgaa agtctgacac ctttacaaca
     5101 agcgatttac gagcaagttt taaaaacgac agtggcgatg tttgcggaaa aatataccta
     5161 tgaagaaaca acgatcttga cgcaagtcca gcagcttcag ttaaaggcaa tcggaaaagt
     5221 accgatagat ctaggttgga agaagttatt tggcaaagag agcgacggaa aagagaagga
     5281 agaagagccg ctacttccta aagtgacaaa aggagaaatg gtgacggtag atctacaagt
     5341 ccttgaaaaa gaaaccaagc caccacaacc ttataccgaa ggtacgttga tcactgcgat
     5401 gaaaacagct gggaaaaccg tggatagtga agaagcgcaa tccatattga aagaagtgga
     5461 aggaatcggt acagaagcga cacgggcgaa tattattgaa accttgaaac aaaaggaata
     5521 tatcaaggta gagaaaaata agcttgtggt gacgaataaa ggggttttgc tttgccaagc
     5581 tgtggaaaaa gagccgctgc ttacgagtgc cgaaatgacc gccaaatggg aaagctactt
     5641 gttaaagatt ggagaacgaa aaggaacaca aaccactttt ctagccaata tccaaaagtt
     5701 tgtctctcac ttattggaag tcgtcccagg acaaatccaa tccattgatt ttggatcgac
     5761 gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg
     5821 tccaaaatgc caggaacaag aagtcttact ctatcaaaag gtggcagctt gtacgtctga
     5881 agcctgtgat tttaaattat ggacaacgat tgccaaaaag aaactcacag cgacacagct
     5941 gaaggagatt attcaaaata gccgaacaag ccaaccagtg aaagggttaa aaggtcagaa
     6001 gggttcgttt gaagcaacga ttgttttaaa agaagacttc actacaggct ttgaattttc
     6061 tgaaaagaaa aaaacgaact tcaagaaaag aacacgacgc acgacaaaat aatggagggt
     6121 taatcttatg cgtaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagccaaaat
     6181 tcgttcagaa caagagaaat tacgtgccag taaaagaaga cttcagcaat taaagaacca
     6241 gcaacggatt caaaaagaaa tcgaattaga tcgatagaca ttaaccttgt tacaaataca
     6301 ctatcatgat atatttaatg tagtgtattt gtaacaaaag ggggggaagt aaatgcaaga
     6361 gaaactgaaa gaattgttgg ctattcgcga tggaaattta tctatgaagg aagccagaaa
     6421 attaggaatc gcagcaacca ctgtacaaag attggtgaat caaggaaaat tgatcaaagt
     6481 tgatcgtggt tattatgtag aggatggtca tggaatagat gatttatttt ggcttcagca
     6541 acgtttttcc aggggaatct tctctcatga aacgacttta gatatttacc agctttctac
     6601 gaatatgccg aaattcattc acttaacttt tccccatgga tataacgttg atcgttcaat
     6661 tacaaaagag cagcagttac aatttcattt cgtgaagaaa gaagtttatg aactaggaaa
     6721 agtagaggga acttcctttc aaggaaatcc tattactatg tatgataaag agcgtacact
     6781 ttgtgatatt tggaatccga gatatgaaat tgaatatgaa atgaaattag aagcattaaa
     6841 agagtacatg gaagacccat tgagagatcc aagtaaatta agagattaca tgacaaaact
     6901 acatgtagaa aaagaaatga aataccacat gcaaagctta tattgatgga ggaaaaagat
     6961 gacaccgaca caatttgcag caaaaacaag aaatttagca aagaggacag gattaaatgc
     7021 acagttagta caacggcatt ttatgatgga tcgtttaatt gaacaaattt ctgaatctaa
     7081 gtataaaaat gatttcattt tgaaaggtgg ctttttatta ggatcaaaat atggaatcga
     7141 tagaaggacg acaattgata tcgacacgac atttagaaat agcaaattaa ctgaagaaaa
     7201 attgaagagt atttttaatg atttaactag gaaacccacg aaagaaggta ttgttttctc
     7261 tattcaagga atgaaggaaa caagagaagc cgatttttat cctggatttc aaattaaatt
     7321 ggtggctaat ttagagaaag tacgtgtgcc atttaaaatg gacgtgacaa caggagattc
     7381 tatttatcct gaagtagata cacactatca ccaattaatg tttgaagata aaaaggtgga
     7441 gattccagct tatccaacag cacaaattat tgcagaaaag ctaagtgcta cgttcagctt
     7501 tggcacagat aattcgagag caaaagatta ctacgactta tttaccattc cgaaactaga
     7561 aaatatcgat acgaaagagt tatacaaaag tgttcgaaat acatttacaa aacgtgggga
     7621 tacaaaaccg ttgtcctttt attatgaaag agaaatggat aagatcatag gaaatgacta
     7681 tttggaaaag cagtgggaaa aatatcgctc agttaacacg tttgcgaatt ccatttcatt
     7741 tacagataca gtcaacgaaa ttgatcacct aatgaaaagt gtgatcaaaa ttgaaaatca
     7801 agaaagaaaa cggcagttta aaagatcaca acaaatgaat caagaaatgg agagataaag
     7861 atgatcaata atgtaacttt agtaggacgc ttaaccaaag atccagattt acgttatacc
     7921 gcgagtggta ctgcagtagc gacttttacc ctagctgtca accgaaattt cacgaatcaa
     7981 aacggcaatc gagaagctga ttttattaac tgtgtgattt ggcgcaaacc agcagaaaca
     8041 atggtgacat tagctaaaaa aggtagcttg attggtgtag ttgggcggat tcaaacgcgg
     8101 acttatgaca accagcaagg acaacgtgtg tatgtgacag aagttgtggc cgataatttc
     8161 caactattag aaagcaaagc agcgacagaa agccgtgcgc acgctgatca atcgtccacc
     8221 agtccaagca ccaccacttt tgaacaacgg gatacagcga cgcctaataa caatggattg
     8281 aacgctttac agaatccatt tgggaatcca tttggtggac aatcaatcga tattagcgat
     8341 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
     8401 aatttagtca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
     8461 tggttttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
     8521 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
     8581 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
     8641 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
     8701 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
     8761 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
     8821 attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat
     8881 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
     8941 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaaaaaag aagagttgca
     9001 atcctaatgt aggactgcag ctctttttgt tttacataga aacaagaaaa atgaaaaggc
     9061 ctttttagga aggcgcgagc gccttctcaa ttgaaaaacc tctctcactc caaccgccac
     9121 cgccgttcga gaagaaagga agtgaacgtt gcacgttcac taagataaag ggtcttgcaa
     9181 agcaagacaa aatagaaaaa acttcctctc accaaaaggt tcgttcctgt tcgttatctc
     9241 tttattgatt tgccagaccg gcaacaagtt gccttttatc accaaaaagc gacaaggaca
     9301 agggtaaatg gcacgctgcg cgcccttgtc cttgtcgctt ttcgttgatt cattctggcc
     9361 aacaaatcaa taaagaaaga actaacgaaa ggaagtaaaa cctttcctaa gaggaggcag
     9421 aatcaatcat gcgaaagaaa tttgattatt ggggtgttcc cttcagcgaa ttattcccaa
     9481 actatcatgc accacatact gtcgaatgcg attgtggaca aagagcgaaa tgtatcaaaa
     9541 gctatcgttt gtatcaatgt ccaacatgcg gaaaaaaata caccttgtct tatggcgatt
     9601 atattttgat tgatgagaaa ggaaagaaac gctgatgaaa acataacata aattattgac
     9661 tgtaaaagaa gtcgttcaaa ttttggtgga caaagaagtc atttctgaaa aagtagcaga
     9721 agctttactt attccgcggt actggacgat agaaacccct ttagtagagg atgcaccgac
     9781 tatgctattt gaagacctag tgaagattgc aggatatgaa ctgcattatc aagaaaacat
     9841 ggaaacttat gaaaacgaat attggttaac caaattagag aactaaaaag gagaaaaaaa
     9901 tatggctcaa aattactatg acgaatttgt caaattacct ttggataaaa tggcacaaaa
     9961 aatggaggat atgacattcc tataccatga gacacgtgtt ccaaaaaaac attataagga
    10021 aagattgtcg gtagctgtgg aagaaatgat tgaatcaggt gtcgaaatga atctcattgc
    10081 gacgtactat cgaacactgg aagaactgaa aaaacaaaat gcaaaatggt ttttccaagc
    10141 cttactatgc ttggaagtag gtgtcaaacc aagcactatt aaaccttctg aatatcaagc
    10201 tttagaacta acgtatggga aatttgtcga aacgaaaaaa gccaagactg tttcaagtga
    10261 atggttagat tattttgaaa atatcaataa gtatggcgcc tattacacca tgaaaaagga
    10321 ggacaacgaa aatgaataag gtgatttatc aactaacaac ggaagatgtg tcacactttt
    10381 taacaaacaa agaacatttg aatgaagtaa tcagacggat tcaaaaagac tttagttgta
    10441 tggaagaaat gaagtacttt attcaaagag tcgaagaaca attgtatttg gaaagtgaaa
    10501 cgagtcaaac gaaggtcaca gtcagcaata attatttaga cgtgtataaa catcgtcctg
    10561 aaacggtaaa aaaaggaacg gagcttctac gactagaaac agaaaaatac atcatgaatg
    10621 tttacctaga aaggagtgaa ctggtcgaaa actttgtcag ctgcacgatt tgcaggaaac
    10681 actattactc agaatcagaa tattattcaa tttttgagag catggaatta atggaagaag
    10741 aaatggaaga gctaaacagc aaaaatgcga cgatagacga ttattatctt gtatttacta
    10801 gatttttgac gtgtggaacg gaatctatcg tgttaatggg aatggattat gaactcgctg
    10861 tacccgaagc atttttgagg gaatatgtag aagaagtaga taacccaggc gacgactggt
    10921 acacgacttt aactgatttt ttagatacct acacttacga cgatacagaa gaaatccaaa
    10981 atgaacttga ccgtaaacga atgccatatc aacgatttga aaggaaaaaa aggcaataat
    11041 catttatacc gaaaagtgag aatgtgtgaa ataagaacaa aaaccttgaa attaagggaa
    11101 tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg tgcgaaataa
    11161 gcgggcaaag cccgataacc taagagaaag aaggagatcc atatgaataa aatcattcct
    11221 ctgttgttcg tactgtgtat tggcgagata cttatgcgtc atggtgcagc tgtacattta
    11281 aacttcgtag tattcttagg aatactagta ttttgtttga ttttttggcg ctttgtgtta
    11341 gcccttgtga gtctactatt caagttgatt gcagccatat ttttcatagg gttatttcga
    11401 tgaaaggaga gagtactaat gccaattgca ataggaaata aacggttacc cgtgacatta
    11461 gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtacggaaa aagtgaatct
    11521 aagattatgt gtattgcgtt agatctattg atcgcgcaag aaaaagcagg atttgatata
    11581 ccagcactaa aaaagtgacg tcacttttca tttgaagaaa caaaagtgat agcacttaat
    11641 tgttcctact atttagaata ttttctatgt cctttttatt ccattttgta tcaataacta
    11701 tgaaatcttt aaagtatcca gatcaatttt tttcttacct gattctaaag attttgtttt
    11761 cttaaacata ttatgatttc ctttcgacta ttttcatata aatgttaaca tagtttatgg
    11821 attcatggaa tgttaatcta atttaataga aaaggataat taagtatata cgttttttct
    11881 tatgtatgtt atactatgta tatacagaaa gaggtgtcta ctatggaaat gttacctata
    11941 aaaaaatggg gaaatagtaa tggccttaga ttgccaaagt atattatgga atatcttgga
    12001 attcatacag aagacaaagt gaagataata caagaagaag taaacggtca gaagcggcta
    12061 attatcgaag cggctaatat agaaaatgaa ttaacaattg aacagttatt tgaaaattac
    12121 actgatgaaa gaaatcatgt gacgattcaa aatttaggtg aagcagtagg taatgaaaaa
    12181 tggtgagaaa acctagacaa ggcgatattc tattattgaa tacagcacct cgatctggac
    12241 acgaacagac aggtaaaaga ccttatattg ttttaagtca tgatattatt gcggattata
    12301 gtaacgttgt tacagttgct ccaatttcta gtacaactag aaactatccg ctatatgtaa
    12361 atataaactc agaatatgaa atgaagacaa caggaaaagt attattagat caactaacga
    12421 caattgatta tgaagccaga gagtgtattt ttttggaaaa agcgcatgat agtttagttg
    12481 aggagttatt gatgaaagtt agaacagttt ttcaaaaagt aagcaaataa aatagaaaca
    12541 gcagcatgat caactattcg tgattgacca tgctgtcttt ttttattcta tatacggttt
    12601 gtctcgtaac attgtagtct tttgcaatct tagcgatcgc aacacctttt tttagatcct
    12661 caacgatgtt tttataaaca agacgacgtt ggggatcttt tgcattggca ctgtatagct
    12721 taggacgtcc tttataaaca ccgttcgctt ttgcaatctt aatgccttgt gcctgacgtc
    12781 gtttagattc ggtacgttct tgttcagcga tcatggccaa aatttgaatg atcaaatctt
    12841 taatgaattt atccaggaga ggattgccaa ttgcttcggc catgatcggt agacttgtga
    12901 tgatcagctg cacatttttc ttcttcaagt aattgactga agcgatgatc tcgtcgtaat
    12961 ttcttccaag tcgatcaata gcttccacaa taaaaatatc ttgatctcgg acgaaatcca
    13021 gtgcttcttg aaaaacaggt cgatgagtaa tcgttgcgcc agattgtttc tcaatgaaaa
    13081 ttctctctgc accgaatttt ttcatttctt caatctgccg ttgttcattt tgatcgatgg
    13141 atgagacacg tatgtatgca actctcaaaa tttttaccta ctttctgcaa ctatttaaaa
    13201 aacaccctaa tcatacacga gaaaaccaga tttttcaatg tcgcggtcgt gtctagttag
    13261 ggta
//
LOCUS       DACCXO010000076        13064 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.077, whole genome shotgun sequence.
ACCESSION   DACCXO010000076 DACCXO010000000
VERSION     DACCXO010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.077"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(61..1539)
                     /locus_tag="IVY70_001969"
     CDS             complement(61..1539)
                     /locus_tag="IVY70_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7959092.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(1837..2772)
                     /gene="murB"
                     /locus_tag="IVY70_001970"
     CDS             complement(1837..2772)
                     /gene="murB"
                     /locus_tag="IVY70_001970"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP7959093.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2845..3765)
                     /locus_tag="IVY70_001971"
     CDS             complement(2845..3765)
                     /locus_tag="IVY70_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7959094.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3878..4630
                     /gene="xth"
                     /locus_tag="IVY70_001972"
     CDS             3878..4630
                     /gene="xth"
                     /locus_tag="IVY70_001972"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP7959095.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5494..6027
                     /locus_tag="IVY70_001973"
     CDS             5494..6027
                     /locus_tag="IVY70_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP7959096.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(6517..7041)
                     /locus_tag="IVY70_001974"
     CDS             complement(6517..7041)
                     /locus_tag="IVY70_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7959097.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(7034..7507)
                     /gene="tsaE"
                     /locus_tag="IVY70_001975"
     CDS             complement(7034..7507)
                     /gene="tsaE"
                     /locus_tag="IVY70_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP7959098.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(7630..8610)
                     /gene="pta"
                     /locus_tag="IVY70_001976"
     CDS             complement(7630..8610)
                     /gene="pta"
                     /locus_tag="IVY70_001976"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP7959099.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(8763..9443)
                     /locus_tag="IVY70_001977"
     CDS             complement(8763..9443)
                     /locus_tag="IVY70_001977"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP7959100.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            9569..10438
                     /locus_tag="IVY70_001978"
     CDS             9569..10438
                     /locus_tag="IVY70_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7959101.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            10464..10979
                     /locus_tag="IVY70_001979"
     CDS             10464..10979
                     /locus_tag="IVY70_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959102.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(11026..11589)
                     /locus_tag="IVY70_001980"
     CDS             complement(11026..11589)
                     /locus_tag="IVY70_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7959103.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(11644..11823)
                     /locus_tag="IVY70_001981"
     CDS             complement(11644..11823)
                     /locus_tag="IVY70_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959104.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(11900..12409)
                     /locus_tag="IVY70_001982"
     CDS             complement(11900..12409)
                     /locus_tag="IVY70_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959105.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(12505..12885)
                     /locus_tag="IVY70_001983"
     CDS             complement(12505..12885)
                     /locus_tag="IVY70_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP7959106.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
BASE COUNT         3812 a         2624 c         2250 g         4378 t
ORIGIN      
        1 ctttgaaaaa aacataaatc agctaataaa tatgtgagac aaagctgcca tccataaata
       61 ctagctgatt ttttcagtta aatgtctatt tttttctctg atgatggtat ttccaaaata
      121 ttcccagtca tctggagtcg gtggattttt ccaaattatc tgttctttat taaatttttc
      181 aagtaaacaa tcagataaat aaatattcgt acgagaagtg attcgactct caatcacaat
      241 attgtaattt ttaaatcctt tcacttgaga aataatgtaa tcggtataag cagatccttg
      301 cgaaaaatca atacagacat gaatcggtgc ttcaaaatat gataccggac aaataccgac
      361 caataaaaag agataatcat aaaataagcg aatcatgaaa cgttcatctt ttcccaagaa
      421 atagcgttta ttctcttgta tgaccttttg gattgctgta ctgaatattg gatacgtctc
      481 gaagaaaaac tgtagctgct ttttagacgt aaagctagtc atctcaaagt cgaatatcca
      541 attttttcga ttgacatgca tcatacccat acgaagttct tttcgtatat atagtctttc
      601 ttcttcagag atttcaatcg aaaatggtaa ttgatcaatc aattccactg acattttttg
      661 actaatcatg tggatatcat gaaaatgagc ttccgcaagt ccaaatgcgc catctaattc
      721 aaattcttct tccgtgatca aaaacaacat gatataagaa atctcatcaa tgatctgctc
      781 tttagggata tgaagaaaag aaatatgtga taaatccttg atgatcttca ttccttctga
      841 gctcttaaag tattcatctg aaaagaaacg ttttccgatt ggttgattcc cataggccct
      901 caacaaacta atagtggtta gcaaattgaa tttgattcgt tttgttgcag acagttttaa
      961 attgaacaga tattcaaaat attgcacgat actctttgct tcattcatga cgctttctct
     1021 aaatggcata gaaaaaccat taaagacccc gaaatatacc ccaaataata cgttccgtat
     1081 gtttatttct tccccaacta atatgttttt ctttaacttt atttgatgct gttcaagaaa
     1141 agagactaat tctcttcttt ttacataggc tcttgatatg cttaaaaaat gattctctgc
     1201 aaatttttcg atcgttactt ttgaatccac gatctccgtt aacagtcgag caatttttga
     1261 tcgttgaaag tagattcttc ttaaattttt gatatccgtc aaattcagac gttcgacatg
     1321 gatctctgct tcttctgaaa tttggatctc tggctttgtt ttgaattcag taaattcttt
     1381 atttaattct atgatataat ttcttgtttt aaatttagac aaacctaatg tttcatataa
     1441 cttaggcagc actacataat tctttttctc aaattctaaa tatctagcta gcagcagttt
     1501 ttctctatct tcctcttcta taaggtaatc caacttcata tcctacacct caaaaaatgc
     1561 ctcataccaa ttacttgata tgcagatgaa agttcatctt ttcacctata ctgattttat
     1621 gatggattat gcttgttttt tcatcagtta ttctcatttt ttatcttttt tttgagaata
     1681 ttgcaaaaaa tggaaaaatt tatgaggaaa tattctatat tttttgtttt attgtgttaa
     1741 aatgtacaag atttgcgcca tttgcgtaaa caaccacaaa aaagagcagg acggccaccg
     1801 tcctgctctt ttacttattt taagttccat tacttattaa ttggctaaac ttctgcttgt
     1861 ttctgatatt gcttgttttt ctccaatgat ccgcacttct gtttctaaag acacatcaaa
     1921 tttttctttg attacttctt ggatgtgcgc aatcaattca atataatctg tcgctgtcgc
     1981 atgatcgata ttgacgatga atccagcatg tttttcagag acttgtgcac cgccccattt
     2041 caggccttgc aatcctgctt gttggatcag ctgacctgta tagtgtccta ctgggcgctt
     2101 aaagacactt ccgcatgaag ggtattctaa tggctgtttt gattgacgca attcggtcaa
     2161 ctcatccatt cttgctttga ttgcttcata atcccctgtt tgcaaactga atcgagcttc
     2221 caatacgata gcacgtaatt cttggacttt actgtgacga taagaaaata ccatttcgtc
     2281 ttttgtcaaa gttttcaatg ttccatcttc aagcaaaaga tccacttcag caaaaacatc
     2341 tttgatctct ccgccatacg cacctgcatt catataaacc gctccaccga cacttcccgg
     2401 gatgccgcaa gcaaattcaa atccagtcaa tgattctgcc aaagctgcat aagtcgtatc
     2461 aatcagtgag gcacctgctt ccgcaatgac catcgttcct ttgacatgaa tctgtttcat
     2521 ttcagtcagc atgatgacta caccgcgaat cccgccatct tgaacgatca gattgcttgc
     2581 atttcctaat accatccaag gtatatcatg gattcggcaa taatcgatga tttgctttac
     2641 ttcttcttgt tttttcggga atgcaaggac atcagctggt ccacctgttt tcgtaaacgt
     2701 atagttcatc aatggttcat caaacaatat agtaatcgtg gctaagcttt caactatttc
     2761 ttctttattc actgaggatt ttcctttctt ttctcaaacg tcctcattat tctatcacgc
     2821 ttaatacaat cttgccagtc ttgttcaaag ttgatgacgg tagtgatctt taagcacttc
     2881 ctcacataat tcatgacttc ttacaacttc tttttgttta agcaattctg tttccgattt
     2941 acctaaacgg atctgagaaa agaaggcctc taccattggc tcaaacccgc gtttgactag
     3001 cgtgtttgtc cagtcgccaa attctttcac tttttttcca tcagcatctt gaatgacaag
     3061 ctgggtcaga tcgtttactt cataagtacc atttttactt gttacttgat atttttctat
     3121 attagcgcca cttgctaaat ccatcgttaa aatagccgtt tgattttgtg tttctaattg
     3181 taaaatggca tattccaaaa attcctttgt ttctcttatc tggtattttg ttctcaaaat
     3241 gggttcatct aataaataca cagctgtatc aataagatgt aaaaacaaat cataaaggac
     3301 gaactctgtc gtttcttttg ccgctattcg atttttttgc aggtggatca accgcttatc
     3361 tgggatacgt ttcaattctt ctaccatagg agcaaagcgc cggttgaaac cgatcatcaa
     3421 aattatttgg ttcttatcag ctaattcttg caattcacga acttcattta gatggacact
     3481 cagtggtttg tcaatataaa catggatacc attttctaga cattttttgg ctaattcata
     3541 atgtactttt gttgctgcgt ggatcataca tgcttcgatt ttttcttcta aaagctcatc
     3601 gatcgtttca tatagatgtt ggaacccgta ctctttttga attctttctt ttgtttcttt
     3661 atttcttgtt gcaaaataaa aatcaagtgt tccttgtttt ttcgcataag ttggtaaata
     3721 tgctttttct gcgatattcc ctacaccgat tacaccaatc ttcatctact ttcctcctgg
     3781 atcatttagt tactttcatc ttacaccatt ttttactaaa atgaggtaca atagtatcgc
     3841 aaaggtaagg aaattaattc gaaggagtgt gcttttattg aaatttatct cttggaacgt
     3901 aaacgggctt cgtgccatcg taaataaaaa ttttttagaa gtttttcatg agttagacgc
     3961 cgattttttc tgtctgcagg aaacgaaact ccaagcagga caaattgatt tagatcttcc
     4021 aggttattat caatattgga attacgcgga acgaaaaggc tattccggca cagcgatttt
     4081 tgctaaaaaa ccagcattga atgccactta cggaatgggt atcgatatcc atgatacaga
     4141 aggccgtctg attactttgg aatattccga tttctttctt gtaacctgtt acacgccaaa
     4201 ttctcaaagc gaattaaaac gtttagatta ccgattagaa tgggaagaag ccttttataa
     4261 ttatttggaa aatctaaaaa aacaaaaacc agtcatcgtg tgtggggatc taaatgtcgc
     4321 tcatcaaaag attgatttga aaaactggaa aacgaatcaa aaaaatgctg gattcactcc
     4381 agaagaaaga gcggctcttt ctcgactgtt ggataacggg tttatcgata cattccgtta
     4441 tttttatcca acccaggaag gtgtctactc ttggtggaac taccgattca attcaaggaa
     4501 aaacaatgct ggttggcgga tcgattactt tttgacgagc aaagatttag agccaggact
     4561 agctgatgca aagatccata cggacattat gggcagtgat cattgtccag tagaattaga
     4621 tttgaaataa agaggttgtg aaaaagtcgt ttagtttcga gtattaagaa aatttttgtg
     4681 aaaatggctt tccacatttt gcataaattt ggcataatac cgagaaactg gcttttgaac
     4741 actgtttatc gagagattgt gaaaaagctg tcctgcatca agtaataaga acagccaaac
     4801 aaaaatagtt cctcatattt ttcgtttggt tgggcttatt accgcagata caagcttttg
     4861 aacaccgttt attaagaggt tgtgaaaaag ctgcactgca tcaagtaata agaacagcca
     4921 aacaaaaata gttcctcata tttttcgttt ggttgggctt attaccgcag atgcaagctt
     4981 ttgaacaccg tttattaaga ggttgtgaaa aagctgcact gcatcaagta ataagaacag
     5041 ccaaacaaaa atagttcctc atatttttcg tttggttgga cttattaccg cagatgcaag
     5101 cttttgaaca ccgtttattg agaagttgtg aaatcagcgt cctgacctga gtattagaga
     5161 aacaaatggc aaaatagttc ctcatatttt gctatttttt gagctaatac cgagggtcag
     5221 ctgattgaac accgtttagt ggagaagttg tgaaaaagtc gcctactttc gagtcgtaag
     5281 aaattttttt gaaaatggtt cctcacaact ttgcataaat ttgacttaat accgagaagt
     5341 catgatcccc atctaactcc aaatcacaag gaatgagttc aaaaactagc ttttctcaac
     5401 aaatattaca taaaaaattc tatgtttttt gttaaattta tttgcttttt tcaaaagtat
     5461 gttacagtaa tttcccaagg aggttttaca gaaatgaatt ttgttgccat ggattttgaa
     5521 actgccaatc atcagcctta tagtgcttgt tctcttgcgc tagtcatggt aaaaaatagc
     5581 cagatcgttg atgaattcta tacattgata cagcctgaga ctcctttttt ctggcgaaat
     5641 gtccaaattc acggaatcca tcaagaagac gtacgcaatg cgccgaaatt cccagaagtc
     5701 tggcaaacta tccaaccata ttttcaaaaa aatcgtttag tcgttgcaca taatgctgct
     5761 tttgatacaa aggtcttagc gggatgctta gattattatc atcttgaaca gcctaattat
     5821 ctttctttat gtacagtgaa aagtagccgt cgcctgtttc ctgaatttcc aaatcatcga
     5881 ttgaacactg tgtgcgaaaa tttgaacatc caattagatc atcaccatga tgcattggaa
     5941 gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa aaaaattcgg gacagatcca
     6001 ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg aaacacacaa agaggtcgtg
     6061 agcctgccgt ttcactccga gtcacaagga gcaatttcaa aaaatagctt ctcatgttta
     6121 ttaaatattg tgactggtga ccgaggagct ggcaggagaa caccgtttat tggagaggtt
     6181 gtgaaaaagc tgttctgcat caagtaataa gaacagccaa acaaaaatag cttctcatat
     6241 ttttcatttg gttggactta ttaccgcaga tgcaagcttt tggacaccgt ttaccaagag
     6301 gttgtgaaat cagcgttttg acctgagtat tagagaaaca aacggcaaaa tagttcctca
     6361 tattttgccg ttttttgagc taataccgaa ggtcagctga ttgaacaccg tttattcaaa
     6421 aaaaatggac tgcgacacga cttttgtcac agtcccttcg tgtgtttttc tgccttctca
     6481 aaaggatgac agtttctcac tcttttccta tttcttctat tcaatcaaat aactcatttt
     6541 atatacatct aaaaattcac ctagatctgt tcttgcccct ctaggcatga ccgcttcgat
     6601 ttggaagccc atttttcggt aaaggtgtac ggctctttca ttacgaactt gaacgtcaag
     6661 ctccaatcga aaaagtacac ccatttcctt cgcccaactg atgatttctt ctagcataag
     6721 agtgcctagt cccattcccc aatattcctg cagaatactg ataccgactt ctcccacgtg
     6781 agacaaacga aattgactgt ctgccttgac agatgctgtt ccaataatcg tcccttcata
     6841 gattgcaagc agcaataaat tattattgct ttcgtatata taatccaact cttcttctaa
     6901 ggtttctggt ggtaacagga gttctgcttc atccaaaact aaaaattctg tctccttatt
     6961 aacttgtccc atcatcaaaa gaatgtcttt tgcatctcct ggcactgctt cccgcaagat
     7021 gatcccaact tcttcactca tgattttcag cccatttttc tagaattcgc tgtttgaagg
     7081 tttcactttg ctccccgtat gagattagtt tcacataccg cttttctaaa tctgtatcac
     7141 ttttttctaa aatcagttcc aaataatcct ccggcagtgc ttcttctaat agatttcccc
     7201 attcaacaac ggaaagccca tcaccttcaa aatattcatc taaccctaaa tctgctccgc
     7261 tagctgctac ccgataaata tccatatgat acaacggaat ccgaccttga ctatattctc
     7321 taataatcgt gtatgtcgga cttttgatca tttgcgttat ccctaatcct tgggcaattc
     7381 ctttcgtcaa cgttgtttta cccgcaccta gatcaccagt caatatcaga ttgtcacctg
     7441 gcattgcgga atgaccaatt gcctctgcga attgttctgt cattgttaat cctgatagtt
     7501 cgatcatttt tcatcttctc ccgctcttct ttctttttgc cagtatatcg cgtaatgaag
     7561 tttttttaaa gaaaaaatga actagtcaag aacagcagct gctgtttttg gctagttcgc
     7621 gtgtatttgt taatcaatca atgtttgtgc tgctgtgata atcgataatt tatatacatc
     7681 ttcttcattt gctccacgag acaaatcaga aaccggtttg ttcaagcctt gtagaattgg
     7741 tccgattgct tcaaagttac cgaaacgctg agcaatttta tagccgatat ttcctgactg
     7801 caattctgga aatacaaaga ctgttgcttg accagctact tctgaatctg gcgctttcag
     7861 ttgtgcaact gaaggaacat aagaagcatc aaattgcaat tcgccgtcga tcatgtattg
     7921 tggcgctagt tttttagcga ttttagttgc ttcaactact ttgtctactt ctggcgattt
     7981 cgctgagcct tttgtcgaga agctcatcat tgctactttt ggttcgatat cgaacaattc
     8041 tgctgttttt gcagaatcta cggcgatttc agccaattct tgcgcagttg gattcacgtt
     8101 gatcgcacaa tccgaaaaga catatttttc ttgatctctt ccacgtacca tgatcatcgc
     8161 tccgcttgtc cgactgatgc caggctttgt tttgatgatc tgaagtgctg ggcgaacagt
     8221 gtcgccagtc gaatggatcg ctccagagac cattccgtct gcaatcccca tatacgtaag
     8281 catcgtacca aagtaattca catcttttaa aatcttttct gcttgttctt tggatgcctt
     8341 tccatttcta cgttcaacaa aagtatccac catttcatcc cacttatcgt aattatcagg
     8401 gctgatgatc gtaaagctag atgttttgat tcctctggca tgcgctgcat ttcttatttc
     8461 ttcttcgttc cctactaaaa tgggttccac aagttcttct gctttcaacc gggcagctgc
     8521 gcctaaaata cgagcatcag tagcttccgg gaaaacgatt ttgatcccac ggcgaacaat
     8581 tttaaatctt aaactatcaa acaattccac aagatgacct ccagacgttt ttatatgtat
     8641 tatcatacac tattccagaa aaaaagaaca gtttatttta tctgtatcag tcaaaaatgc
     8701 tggaaaaatt aagtttttca catacaaagc gaactgcact ataacaggaa agcgctgttt
     8761 tattacactg tttcaggcag ttgccaatca atcggttctt ctcctaacgc aaggagcgca
     8821 tcattcgttt tcgagaaggg tttggaacca aaaaagccac gacttgcaga taacggactg
     8881 ggatgtggag atgttatgat gatatggcga ctcttatcga tcattttgat tttttcttgt
     8941 gccggttttc cccacaagat aaacacgatt ggtttttccc gttcatttaa tttttcaatg
     9001 atcgtgtctg ttaatctttc ccaacctttc ccgcgatgag aatacgcttg cccttcccga
     9061 acggtaagaa cggtgttgag taaaaggact ccctgtttag cccatgacac tagatttcca
     9121 tgttgaacag gtgcgattcc aagatcatca taaagctctt tatagatatt tcttaacgat
     9181 ggcgggatct ttacgccagg ttgcacagaa aaagataagc catgagcttg attttctcca
     9241 tgataaggat cttgtcctaa aatcaccact ttaacttctt cataaggagt caattccaat
     9301 gcttcataga tatgatacat atctggatgg atcttttggg acgcatattc tttttttaaa
     9361 aattctctta gatgcagata ataatctttt gaaaattctg aggacaaaac ttcttgccaa
     9421 ctgttatgaa tgatttcttt caaaaactcc acctcctaat aaaaagaata actcacattt
     9481 tcttcaatca caagggacaa tcttcaccct cagcacttga ttaatatggt aaactaggaa
     9541 caagaagcag aaaggaacga taatcattat gatcaaatta attgcatcag atatggacgg
     9601 cacattatta gatgcccata tgagtgtttc tcctgaaaat gccgaggcga tacgttttgc
     9661 aaatgatgcc ggtgttgagt tcatggtcgc tactggtcga aactatcaag aagctcgcgc
     9721 agctttagat gaggtaggaa tcgattgtgc gatgatcact ttgaacgggg cacaggtatt
     9781 tgataaagaa ggaaattcat tgtttaccgt gtcgattcca aataatcaag caatctctgt
     9841 tttagatatt ctagatgcta acggtattta ctatgaagta gcaaccaacg atggtcttta
     9901 ttctgaaaat caagcgaaac gaattgaaag ttttgcttcc atggtggcta cccatttacc
     9961 tcaccttacg tataaaatgg cgattgccat ggcctcagct aagctggagt tattgcacat
    10021 cacatacgtg gacagtattc gtgctatttt ggaagaaaag aatctggaag ttttaaaaat
    10081 tatttgtttt catacagaag ggccagctgt attaggtcca gttggaaagc agatttctct
    10141 tttaggcgat ctagccgtta cttcctctgg tcaaaacaat atcgaagtga accataaaaa
    10201 cgctcaaaaa ggaattgctg ttgcccatgt cgctcacgag cgtgggattt ctttagaaga
    10261 agtcatgacg attggcgata acttcaatga tgtcagcatg ctgcaagtag caggagtcag
    10321 tttcgcaatg ggaaatgcag aaatcgaagt aaaagattat gccaaatata caacggacac
    10381 aaaccttgaa tctggcgtag gaaaagccat attgcgcgcg attgcagaag acttgtagtt
    10441 ttatctagaa aagagggata tagatgtcag aatttttcat tcaggaaaag cagcttggac
    10501 gtgcaactcg gacaattgtc aaaaacgaaa aaggacattc cctgtttttg atggttggtc
    10561 gctggggaac aaagggcgat gcattgtctc tttatgccat gaatggcgat ctcgttgctc
    10621 acatcaagca gataagcctg acattcggta cgcgttttga gctgtataaa gagttcgaaa
    10681 aggtgggaac catgcagaaa atattcaatt ggcccggtga cttttattat attcgtagac
    10741 ttcactggac agtccaaggt gatatctata atcatcaata tcaaatccat catttcaatc
    10801 agcaaatcat gaaaatggat aaagcaacac ttttttcagg agattactat gtattggatg
    10861 tctcaaatga tgaagatgct ccagtttgta tttgtattgc tgcggtttta gattactggc
    10921 tctaccgtaa agacaaaagt cgcgataagc ctgtttctta ccgacttaac ttaagttaaa
    10981 aagatgtacc tgaaatgcgg aaatacgcat ttcaggtaca tctttttaga tattcttttg
    11041 catatgatca taaagatgac cactgatcat ccgctgaccg acaactttga agtcatggcg
    11101 ttcgtataat tttttagcct ttggattttt ttcatccaca cttagaccga tgacggagcg
    11161 attagctttc ttcgccaatt taggtaatgc ttctaataat cgcgaaccga tgccttgtcc
    11221 tctaaaatct tctgatacac tgatagagtc caaataccat tcgttgggaa acgtttcttt
    11281 atcaatgaac aaacgtgtag acgcatctag ctgatatttc tctaagatag gtaccagtgg
    11341 ctgatcgatg attgattctt cttcatctgt ataaccgtaa gcaactcctg caactttacc
    11401 gtctatttct tctacgatac ctcgtgaata actgtaacga taatttggat cagtgattgc
    11461 ttcttctaaa atctctaaag tcttttgttc ccctgtctgc ttcaaaaatg gcagttccat
    11521 atcttttaga atcactaaga tcaatggggc gatttctgga gcatcttctt ttctcgcaaa
    11581 acgaatcata gtttcctctc cttttctcca ttctaacatg cagaaaaaga aatcgcaaaa
    11641 tcgttatttt aaaaaatctt caatcttttc agtaatgaaa taaccatgag taacgattct
    11701 tgccgcttca gttcggcaat aagggcagac gactggcatt tctttatcat tatgttcgac
    11761 tacattattg acttcttcgt atgcaaattt ttttccgcaa ttagggcatt tcattttttc
    11821 catgttatct caacttcctt tcatctttac tctcttagct tactcttata ttgtaaaaaa
    11881 gagaagcaat atccttcttc tatgcagata tccatacctg acggtcctgt tctccatcta
    11941 ttaatcggac tttataataa ggacgttcat ctttgactag aagtgtccac tctttcactt
    12001 ttgctttttt cccgtagtag ctgactgctt ccttcatcgc tgcttgcggt tgtttgaact
    12061 cttccgtttt ttctatatca aaaagatgtt ctgcggcctt ttccgtgtca tttcgtacgg
    12121 ttgttttccc agtggatgga ttgactgcta tgacttgtcc ttcacaaaaa aactggtatt
    12181 catatgttgc attcgtagga ttctccaaat ctttttcaac tgcaaaatga atagcagata
    12241 tgttatgttc ttttgtttcg ttttttacga ttgctgctgc ttttttcaat gagatccgat
    12301 attcctggtc actaatatca tccatgtgaa aagcggcatt gagcagtccc gtcataccgg
    12361 taatccctat tacgatgagg gcagcaacac caaacatcat ttttttcatt cttcattctc
    12421 ctgtttcttt tttttaaaga atatcaagaa atgaacgaat gaaaaatcga atgaaacgaa
    12481 aaaaaagtga caattttgtt atctttattc gtgcagttct aatggcaatc cgtctggatc
    12541 aaagaaaaac gtcatttttt ttcctgtaaa ggtatcttct ctaatcggtt ctgattcgat
    12601 ccctaaggac tgcaattctg caattgtttc ttcaatatcg tctactttga aagcaagatg
    12661 acgcaagcca caagcttctg gatagtttgg tcgttgtggc gcattagcaa ttccaaaaat
    12721 ctccaattca gcacttccta gtttcagatc taatttatag tcatctcgtt ctttgcggta
    12781 attttctctg attacttcaa atcctaattt attaacataa aaatcacgtg atttctgata
    12841 atctgaaaca atgatggcta tatgatggat cgttgtaagg ttcatatgag cactcctatt
    12901 cttcgtttaa gctcggatct atattaatct ccagttccat tgtaaatgtg tcagattgaa
    12961 aaaaatagct gtttttcctt tttataagaa aaaaggcaaa aaaaacagaa agtaggtcct
    13021 ccaagacact cttcctgttc ccaatataat aaatgaaagt aaga
//
LOCUS       DACCXO010000077        12876 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.078, whole genome shotgun sequence.
ACCESSION   DACCXO010000077 DACCXO010000000
VERSION     DACCXO010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.078"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            131..3658
                     /locus_tag="IVY70_001984"
     CDS             131..3658
                     /locus_tag="IVY70_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP7959107.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3655..7377
                     /gene="addA"
                     /locus_tag="IVY70_001985"
     CDS             3655..7377
                     /gene="addA"
                     /locus_tag="IVY70_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP7959108.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7446..7787
                     /locus_tag="IVY70_001986"
     CDS             7446..7787
                     /locus_tag="IVY70_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7959109.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8096..9139
                     /gene="pheS"
                     /locus_tag="IVY70_001987"
     CDS             8096..9139
                     /gene="pheS"
                     /locus_tag="IVY70_001987"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP7959110.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9144..11564
                     /locus_tag="IVY70_001988"
     CDS             9144..11564
                     /locus_tag="IVY70_001988"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP7959111.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="IVY70_001989"
     CDS             complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="IVY70_001989"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP7959112.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4370 a         2240 c         2733 g         3533 t
ORIGIN      
        1 tttgcgtata cttaaaaaag tagaaatatc agaaccttaa aaggatataa tttgaagaat
       61 atagaacaaa tgttcagtat tttttgatat aataaaggag caccgtatca tgtgttgaag
      121 gaggaaaagc atgtctttac aatttattct tggaaacggg aaaaaagatc accgtcaagt
      181 cttgatcgac gaagcatccg cttggctcga ccaggatgca agtaaccaag tatttttcct
      241 tgttcccaac tacagcaaat tcgaacagga acaagaaatt ttagcggaga tgcgaagaag
      301 aagcggaaaa aaaactttca gcacaacaaa cgttcaagta tttagttttt atcgtctggc
      361 ttggtatttg ctgcaacaga caactcttct tacaggaaat gaattatcag aatcaggatc
      421 tgcaatgatc ttgcgacaga ttttagaaaa aaatgaggaa gaattaacaa tctttcgcgg
      481 agaaatcaat aaaagagggt ttattcgtca attacaagaa ctctacagtg aattgcgtac
      541 aggaaatatt caaccagaag acctgatgct tttatttggt gattcaccta atgcagaaga
      601 ccagcaactt aaaatgaaag acttgaagct gattttttca gcttatgaca gcgaattgac
      661 tcaacgtgca ctaacaaatg aagatgcact atctatacta gcaaattata tgcagacaca
      721 agatttcaac aacgtcaaat ttattgtcag tggtttttct cgaatcaatg gtcaagagta
      781 tcaattactg caggcaatga tggaaaaggg gcaattggtt gtcgatttat tgctggatcg
      841 tccttataca gcggatcttc cagaagtact tacgctcttt catgagactg gaaagcttta
      901 tttccgtctt tttcaaagtg caagagaacg gaaagtccct atattagtgg accgatttgc
      961 accagagcag actcgtccga cagagcttca actgctgcaa acaatgtggg aagagacatc
     1021 tagccaaaag aaagcggcag tacacaaaat agggggacaa gagcatctcc atttgtggga
     1081 agctgaaaat ccaactgaag aagtccgaca attagctgtt gaaatcagac gactagtcag
     1141 tgaagaaaac taccgttatc gtgatatcca agtgctgaca aaaaatatgt ctttatatgg
     1201 aaatatcctt cggccgatct ttcaagaaat ggcaattcct ttttatatcg atgaagaaca
     1261 aatgatggaa aatcatccgc tgatcgaatg gatcaacagt ctgttcgctc ttgaccgcta
     1321 ttcttatcgg ctaagtgata ttatgcgttt tttcaaaact gaattatttc tgtcgacgga
     1381 agcagaaacc gaacttgatg aatggaaaca agagcgagat gaattcagga aaaaactgga
     1441 catcactgaa aatgtagcac ttgcttacaa ttatcaagga acttattgga ctcgtgaaaa
     1501 agactggcag tttgtggcgt atgattttga agcagaacag attgaagacg tacagtattt
     1561 ggaagaacag tcaaatacga ttagacgatt atttcagaaa gctgtaccag tatttttgaa
     1621 aaaaataaag aaaagcaaaa caggacaaga ggccgcaaat gttttttatc aatttttgat
     1681 ccaaagtggc gtacagcaac aattattgtt ttggcgtaat caggaagtag acagaggaaa
     1741 cttagaagcg gcaagaaacc atgagcagac atggggagcg ctgattgatc tgttggatga
     1801 atatgtagta atttatggtg aatcggcttt tgactggtca ctgttccaag aaatcattac
     1861 aagtggtcta gaaaatctaa gttatggtaa aatccctacc gcaatcgacc aagtgcgaat
     1921 caatcgtttg gaattagttc gaccaaatca agctgccgtc acttttgcat tagggttgaa
     1981 tgatcaagtg tttccagacc gaaaagagac aaaaggattg ttgtccagtg aagaacgaga
     2041 agtattaaat caggccttgc cagatggaca gtttttattc gacccatcaa aggaaagtat
     2101 ctcttttgag cctttccaag cctatcttgt ttttctttcc ggaacagatc gattatatct
     2161 tagttatgca caaagctatg acacagatgg ttcgctgaag atgtctcctt atttgcgtag
     2221 aatcacagaa tatttatctg ttcccgtcga gagaaaagag catttgacga tcgaaagcga
     2281 ccctaattgt tatgtaggga cttacagaag cggaatcaat acagtcaata ggatttatcg
     2341 attagcttta gatgaaaaaa aacctgtacc aaagcactgg caaaagctca aagaattagt
     2401 actgaattcg tcatggcgaa attttgcttt acagattttt gaaagtcagg aacatttaaa
     2461 tgttcctgtt tcgatgccag aaaaaatggc ggaagagctt tatggaaaag agatttatac
     2521 ttcaatttca cgtatggaga atttttataa ttgtgaatac aaatacttca tgcaatttgg
     2581 tttgaaattg aaagaacgaa ctgtttatgg tctaactcca gcagcaactg gtgattttta
     2641 tcatgaatca ttggatcgtt tcttcaagct actgttttca aaccaattat ccttagtgaa
     2701 tatgtccgat gcgcagcgta gagactttac tgaaaaagtg ctgcaggaag tattcggaga
     2761 acttcggttt gagatattgg atagttctgc aagaatgaac tatatccgct accagttagg
     2821 acagacgatt caaaaagtga catgggcttt gcaaaaacag agcaaaaata caggcatgca
     2881 tcctcttcag acagagattc tatttggtca gattgctgga gcaaaaggta tcacaggtct
     2941 ggaattaccg ttgagtaatg gtggaaaact ccatgttcga gggaaaatcg atcgaatcga
     3001 tgtagcttct gaacaagaag atacttggct aagcgtcgtt gattacaaat ctagcggacg
     3061 ttcattcgat gtcacagaag cttattatgg catggctatg caattattga cgtatctgga
     3121 cgtagcattg atggatgcgg tacgtttgac tggaaaggcg gctgttaaac cagcaggatc
     3181 atattattta catgttcata atcctgtatt gacggagaca gatgatgtag agaagaatac
     3241 attgaaaaaa tttagttatg atggcgtttt tgtcgatgat cccgctcttt tagaagtact
     3301 agatcattca ctggaagcaa aagaacaatc actgatctat ccagtgaaaa aaaatgcgaa
     3361 agatgaatac caaaaaggta gtttcagtaa agacaaattt ttcacggaag aagaactgca
     3421 actattcatg gcgcataatc gggaaaatat gcggttagct ggagatgcga ttacttccgg
     3481 agaaatcaaa ctaaatccag cttataaaga taaacagcgg attgcctgcg aattttgtcc
     3541 attcagaagc atctgtacgt ttgatgtgat gctgaaagaa aataattacc accgtctgga
     3601 gaaaatagat aaggaagaag cactgcgtag aatattgaaa aggagtgaag aagagtgaat
     3661 caaattcctt taaaaccgca aaatgaacga ttcacagatg atcagtggca agcgattttt
     3721 gatcaaggag ataatctatt agtctctgcg tctgccggat caggtaaaac tacagtgctt
     3781 gttcgaagag tgattgaaaa actaaaaatg ggttttgata tcgatgaatt attgattgtg
     3841 acatttactg aagctgccgc aagagaaatg aaagaaagaa ttcaagaggc attacaagaa
     3901 tccgtcaata gcgaaagtga ccctgtacgt agacagcatt ttactaagca gttagtttta
     3961 ttgcccacag cgaatatctc aaccttacat gctttttgcc tgacagttat tcgacgttat
     4021 tattacttaa ttgatattga tccagtcttt cgcatgttaa cagatgaaac agaaacgatc
     4081 ttgatgaagg aggatgtgtg ggacgagctg agagaagcac tttatgcaga aaatgatgaa
     4141 cgatttttcc aattaacaat gaatttttct aatgatcgtt cagatgacgg cttgacgaat
     4201 ctggtgtttt ctctttatga gtttgcccgt gccaaccctg acccccaaaa atggctagaa
     4261 cagttaagcg ataattatcg tttgcctgag gggctagcga aatctcgatt atatcaaaaa
     4321 caaatccgtc ctttagtttt ggcagatata taccaatgtg tacagttata tgaacagatg
     4381 actcagttag cacaaggtga aggattagaa aaaatgcatg agcaagtagc tggagaacaa
     4441 cagcagatca agaatatcta tgaagcgttc tcgcaagatc ggttagaaga agcttatgct
     4501 ggtttggaac aactaacttt ttcaactttt aagagcagtc ggaaagcaga attgaaagaa
     4561 atttccaatg aagtcaaggg catgagggat aaagcaaaaa aactgattca gcagataagt
     4621 aaaagttatt ttcctgtttc cccaagccaa atggaagaat taacggacaa agcactaccg
     4681 ttagtcgaag aaatgacaaa agtcacgcaa agcttcatgg acggtttcag tatgcgtaaa
     4741 cgggaaaaag gtgtgctgga tttcaatgat ctggaacatt tagcgttgca gattctcaca
     4801 gaaaaaacga aagatgtatg gctgccaagt gaagcttcaa aacattatcg aaaaaaattc
     4861 aaagaagtaa tggtagatga atatcaagac gtgaaccaat tacaagaagc cattttatac
     4921 tggctaagag aacccgatga tacaaaagga aatatgttca tggttgggga tgtcaaacaa
     4981 tccatctatt ctttccgttt agcagatcct agtttattta tcgggaaata cgagaatttc
     5041 tcaaagaaag aaggcggacg tcgaatcgtt ttggctgaga acttccgctc tagaaaagaa
     5101 gtcctttctt ttacgaatct tatttttgaa caactaatgg atccagcagt gggtcaaatc
     5161 aattatgacg aagcagccaa actgatccaa ggattttcag actttccaga gaatgaacag
     5221 tttgagccgg aaatcatgat ctatgaaaaa gaacaggaag aatcagagat tgagattccg
     5281 acggatgata tactggaaga caaaacagaa ggcgaacttt ttatgacagg actaaaaatt
     5341 cgtcaattaa ttgattcctc ctttatgatt tatgataaaa aatcaaaaaa aagtcgtccg
     5401 atagagtaca aagacattgt tctcttgaca cctacaaaga agaacaactt aacgatttta
     5461 gagattttca aaacattgga tattccatta gagatgaatg atgctcaaaa ctattttcaa
     5521 gcgacagaaa ttcgcacgat gatctcgctg ctacaattaa tcgataatcc ctatcaagat
     5581 attccgcttg cggctgtatt gcgctcaccg atcgtgggtc tgattgaacc agaattagcc
     5641 agcattcgat tagctaaccg tgcacatact tactatgatg ctgtgcttgc ttatcaagca
     5701 tcaaacgaag atgaattagc agctaagctg gaacattttg gcaagcaact agagcattgg
     5761 cgagaacttg ctcgccgttc ttcgatcact gacttgttat gggatatcta ttatgagacg
     5821 ggatatctag agtatgtggt cggtctgcca gcaggggcac agagacaagc caatctttat
     5881 gcattggttg atcgtgccaa agcttacgag caaagttctt tcagaggatt ataccagttc
     5941 gtgcgtttca ttgaaaaaat gcaagaaaaa gataaagact tagcagaacc agtcatttca
     6001 atagaagata atgctgttcg cgttatgacg atccatgcaa gtaaaggctt ggaattccca
     6061 gttgtatttt tgttagatat gacaaaggag ttcaacttac aagacttgcg caatcggtat
     6121 gcttttgaag aaaaacttgg agcaggaatt cgctatatgg atcctgaaac acgagtgtta
     6181 tatgatacgt taccgtttca agccatcaaa ttggcaaagc aaaacaaatt gttgtcggaa
     6241 gaaatgcgta aattgtatgt aggattgact cgggcagaac aaaagctttt cattgtcggt
     6301 tcctataaaa ataaagagca aatgatccaa acgtggtctg aagcagctga tcatgaggag
     6361 ttagtttttg atcctgcgct acgtttgaag ggcagaagca gcttgatgaa ttggattggt
     6421 tacggattga tccgtcatcc tgaaatgcag aaatacttag aggaagaaat ttcgacttct
     6481 cttcttcaac attcaaatgc acagttttct atttcatgga tgaatcagca atcgatcatt
     6541 gaacaaagac agttgcttgc tgaaaaagaa ctggttaact tagatcagca gatgaaagaa
     6601 gatgaaactt tattagcaga ttcgttacaa aaacgtctgg catatgaata tccttatcaa
     6661 gcttcttcac aaaccacaag ttatcaatct gtatctgaaa tcaaacgatt attcgaagat
     6721 ccagatgata cacaagaatc acgtttgact ctggaaagca gtcagaacaa agcagcatcc
     6781 cgtcaatttc gttatacgca agaacaacta gcagaaccaa aatttttgca aaaagatcgg
     6841 caagtgtcag cagctgctgt tggaactgct acgcatgctt tgttacaatt actgccatta
     6901 gagatgccaa ctacagagag tatccatcaa aaattacagg aattggtaag gaaacggtta
     6961 gtagacgaaa aagtcgcgaa aaaagtagat gtttcttcta ttatatggtt ctttcaaaca
     7021 gaacttggtc aacagctgat tgccaacaaa gaaaacgtaa aacgagaaca acctttttcc
     7081 atgctgcttc ctgctgacga agtatttcaa gactatccaa atcaagaaga tgaactatta
     7141 atccacggaa tcgtcgatgg atacctagaa gaaaaagatc atctcaatat ctatgatttt
     7201 aaaacagatt ttattttaca tccggatgat ccagcagaaa tagatgcaat cgtccaaaaa
     7261 tatcaaggac agctaagact ttaccaacaa gcaatgtcag aagctttgaa taagccagta
     7321 gaaaatgttt tcctaatttt gcttcgagtg aaacaaatta taaacattaa caaataatta
     7381 taattgagca aacttgactt atgttttgta aggattatgc tatactctgg gaagaggtga
     7441 gtatgatgga gctaacacag acagaattcg ttttatgccc taaatttgaa aaaacattct
     7501 ctattttagg aaaaaaatgg aacggattga ttatcgatgt actactcgaa aacggtccgc
     7561 aacgctttgt tgagcttgcg gcaaaaatcc cgatggtcag tgatcgagta ttagtcgaac
     7621 gcttgaagga attagaaaaa gaaggaatag tcactcgaaa ctttgcctgt agcgaaaaga
     7681 gccgttcaga atatctcttg actgaaaaag gagcagactt gcaaaaagcg atgcgtcaaa
     7741 tacaagtttg gagcgaaaaa tggatcacag aggaagaatg cggttgattt tgtaaaaaaa
     7801 atcttgtaaa ctatagtcca tacgaataag taaagaacga tgacgaaaaa gagtagtttg
     7861 gctaaaccag ttcagggaga tctgtcgcag actgaaagca gatcctgtgt ttgaaaaatg
     7921 aagttcactt tccgagttcg tctgataaaa tcagaccggc aattgtcgtt aacgaagtaa
     7981 gagtgcggtg taagtcattt atccgcaaat taggatggta acgcgaagac ctcgttcctt
     8041 aacaggaatg aggtcttttt gttttgtatt tggaatttaa aggaggaaca agcacatgtc
     8101 tctacaagca caattagaga gcttgaaaga agcaacgctc gtcaaaatca cagcggcaca
     8161 agatttaaat gaattaaatc aaatacgtgt tgaaacgtta ggaaaaaaag gaccgatcac
     8221 agaagtatta cgtgggatga aagatctaag tccagaagag cgaccaaaag tcggaagttt
     8281 cgcaaatgag atccgagatc ttttgacaga agagatcgtg cgaaaaaaac aagtattaga
     8341 agaagaagcg ttaaatgcag ctttggcaaa agaaacaata gacgtgacac tacctggtag
     8401 aaaagtaaaa catggtacac gtcatatcgt gacacaaatc atggaagaaa ttgaagatat
     8461 ttttgtgggt atgggctatc agatcgtaga aggatatgaa gtagaatctg atcattacaa
     8521 tttcgaacgg atgaatctgc caaaagatca tccagcaagg gacatgcaag atactttcta
     8581 tatttcagac gagatattga ttcggacaca tacttcacca gtccaagcac ggacaatgga
     8641 aaaacatgat ttctctaagg gtgctctacg aatgatctcg ccagggaaag tcttccgcag
     8701 agatacagat gatgcgaccc acagtcatca gttccatcaa attgaagggc tagttgttga
     8761 taaaaacatc acaatgggcg accttaaagg aacattagaa gtcgtaatga aaaaaatgtt
     8821 tggggaagat cgtgaaattc gtttgcgtcc aagttatttc ccatttacgg aaccatcagt
     8881 agaagtagat gtcagctgtt tcaaatgtgg tggtgccggt tgtaacgtat gtaaatacac
     8941 tggatggatc gaaattttag gagctggcat ggtacacccg aatgtgttga agatgtcagg
     9001 aatcgatcca gaagaatatt caggctttgc tttcggcctt ggaccagacc gcgtagctat
     9061 gttgcggtat ggagtgaatg atatccgtaa tttctatcaa aatgacttac gtttcttaag
     9121 tcagttcaag ggggagtaag aaaatgttag tttcatataa atggctcaat cgttatgtag
     9181 atttatcgaa tgtaacacca aaagaattag cggataagat gtctgtgaca ggaatcgaag
     9241 tagagggagt aacggttcca gaagaaggct tgaaaaaaat cgttgtcggt gaagtcaagg
     9301 aatgcgtacc acatccagat tctgatcacc tttctatttg tcaagtcgat attggcgaag
     9361 acgaactgtc tcaaatcgtt tgtggagcgc ctaatgtgaa ggttggaatc aaagtgattg
     9421 ttgcacttcc taactcacgt atcgctggta atgtaaaaat caaaaaagga aaaatgcgtg
     9481 gccaagtatc aaatggcatg atttgctcat tacaagaaat tggctattca gatagtgtta
     9541 taccgaaaga atacgctgaa ggtatctatt atatgccaca agaagctgta aatggcgatc
     9601 cagtttttcc ttatttggat atggatgatt cgattattga attatctatc acaccgaatc
     9661 gtgcggatgc attgtcaatg cgtggcgtag cttatgaagt tggggcaatt tatcgtcaga
     9721 ctcctacatt cgatgacgaa aagctggtag aatcctcacg tcctgctagc gataaaattc
     9781 aagtggaagt tgaagataaa gaagcagttc ctgcttatca aatcagaatc attgaaggag
     9841 taagaatcca gccaagtcct caatggctgc aaaatctttt gatgaacgaa ggcattcgtc
     9901 caatcaacaa tgtagtagat gtgacgaact atattttgct tttattcgga caaccactac
     9961 atgcatttga ctacgacaaa ttgaaatcaa aagagatctt tgtccgccgt gcaaaagaaa
    10021 atgaaacatt agtaacacta gatggagaat ctcgggaatt gacaactgac aatctcgtca
    10081 ttactaacgg caaagaacca gttgctttag ctggtgtcat gggagggtta gactcagaga
    10141 tcacgaatga aacaacaaca gttgcactag aatctgcttt atttgatcca atttcgattc
    10201 gtcgtacagc aaaacaattt aatttgcgta gtgaatcctc tgcacgtttt gaaaaaggaa
    10261 tcaaccatgc gactgttgga gaagccagtg aggttgctgc agcaatgatc gtttctcttg
    10321 cgggtgggga agttctacaa ggcgcggtca aaggctcaga gatcaaagca gaagacgtat
    10381 cagtgaagat ttcacttgaa cgcatcaatc aatatttagg aacatctctt gtcgttaaag
    10441 aagtaaatga gatttttgaa gcacttggat ttagctatga agaaaaggag tatgtttatt
    10501 ccgtagtagt accaccaaga cgctgggata tccaaatcga agcagatatt atcgaagaag
    10561 ttgctcgtat ttatggatac gaccagttgc catctacact tccaagtgga gaaacggttg
    10621 ctggaagctt gtcaaaagaa caagaaacta ctcgtaaaat tcgtacaatc tttgaaggaa
    10681 gcggattgag cgaagcaatc agttatgcat tgacaacaga agaaaaatca agacagtttg
    10741 ttataaatga aagtaatatt acacgtttgg actggccaat gagcgaagaa cgttctgtat
    10801 tacgaatgaa tttgatcagc ggactattag atgatatcag ttataacact gcccgaaaaa
    10861 atacagagat tgctttatat gaaattggtc gggtgttctt ccaagaaaat gatccacgta
    10921 cacatctgcc gcaagaagta aaacatgcag caatcgctat ttctggtatt tgggaagaaa
    10981 aagattggca gacgaaagct caaaatgtag actttttcac catcaagggt cttttagaaa
    11041 acttatttga acaactaggc atcactgaag atgtccatta tcaaacagtg acagaaatga
    11101 aggaaatgca tcctggaaga actgctgcga tttaccttgg cgaaaaattt attggttttg
    11161 tcggtcaagt gcatccaacg attacaaaaa gttatgatat tccagaaaca tatgttgctg
    11221 agtttgattt atctgctata gtggaagctg ctcaaaaagg gatcgttttc gaagcagtga
    11281 caaaattccc agcagtcagc cgggacatcg ctttgcttgt aaaagaaact gtgacaaatc
    11341 aggaattaac agaagttatc cgttctgctg ccggaagatt tttaacagat atccaattat
    11401 ttgatgtcta tcaaggagaa aacattgaaa aaggacataa atccatggcg tatagcctga
    11461 cattcgtcaa cccagaagca acattgacag atgaagaaat caataaagca atggaaaaag
    11521 tcgaaaaagc gttggtagaa aacctagagg caagtattcg ctaagaagga aaatatagta
    11581 agtaacgatc aagtagcttt tgaagaccat acatttgaaa aaaagatgtg acacaacttc
    11641 gtcacatctc tttttttgat tttaaaaaag gaatagcaca gagacgagat aaaaaaacag
    11701 gcctttacag cctgcttttt tagagtaaat gaattaatgg aagtaattga ttcccattgc
    11761 tgattttact tcagaaagtg tttgtgcagc tactttagca gcttcttcac tacctttacg
    11821 taagatttcc ataacagctg aaggatcttt ttccaattct tctcggcgcg cacggatcgg
    11881 tgcaaattca gcttccaata catcaatgag gtatcgtttg atctttacat caccaagtcc
    11941 gccgcggcgg taatgagctt tcatttcttc aattgcttct ttatctgttc cgaatacatc
    12001 caagtaggtg aagaccatat tcccttctac ttgtccagga tcttgtacat ggatgtggtt
    12061 tggatcagta tacatactca tgactttttt ctgtaagaca tcggcaggat cggagatata
    12121 aataccgttt cctaacgatt tactcatttt gccatttcca tcgattccag gtaatctacc
    12181 cattcctttt ggtgggaata cgccttttgg ttccaccaag acttcaccat atgtatgatt
    12241 gaagctttgt acgatttcct gtgtctgctc tagcattggt ttttgatcct caccaacagg
    12301 tacaagattc gctttgaatg cagtgatatc tgctgcttga gaaacaggat agataaagaa
    12361 tcctgtaggc acgctttcac cgaatttttt ttgttcgatt tctgttttga cagtcggatt
    12421 gcgccgaacg cgtcctacgc tgactaagtt caaataatac atggttaatt cagctaattc
    12481 cggaatttgt gattggataa ataaagtaga ttttgcagga tctaatccca cggctaaata
    12541 gtctaaagca acttgcaaga catttgatga aaccttctca ggatttttag cattatctgt
    12601 cagtgcttgc atgtcagcaa tcatgacaaa cagttggttg tcttcatctg cctgcatagc
    12661 cactcgggtt tttagagaac cgacgtaatg ccctaaatgc agcttcccag ttggtcgatc
    12721 tcccgttaaa ataatatttt tcaaaaaaga cacctcttta cgatttgtaa gtctgagcac
    12781 ttaatatctt accgtttttc ctgaaaagaa acaacagttc ctaaggaaaa aacaaggaaa
    12841 gaggataaaa taactaatag attagaaatg gaagaa
//
LOCUS       DACCXO010000078        12792 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.079, whole genome shotgun sequence.
ACCESSION   DACCXO010000078 DACCXO010000000
VERSION     DACCXO010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.079"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            105..1148
                     /locus_tag="IVY70_001990"
     CDS             105..1148
                     /locus_tag="IVY70_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HAP7959113.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            1321..1545
                     /locus_tag="IVY70_001991"
     CDS             1321..1545
                     /locus_tag="IVY70_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959114.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            1548..1805
                     /locus_tag="IVY70_001992"
     CDS             1548..1805
                     /locus_tag="IVY70_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959115.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            2022..2897
                     /locus_tag="IVY70_001993"
     CDS             2022..2897
                     /locus_tag="IVY70_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959116.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            2907..3701
                     /locus_tag="IVY70_001994"
     CDS             2907..3701
                     /locus_tag="IVY70_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959117.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            3730..4848
                     /locus_tag="IVY70_001995"
     CDS             3730..4848
                     /locus_tag="IVY70_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959118.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            4881..5201
                     /locus_tag="IVY70_001996"
     CDS             4881..5201
                     /locus_tag="IVY70_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959119.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            5456..6145
                     /locus_tag="IVY70_001997"
     CDS             5456..6145
                     /locus_tag="IVY70_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HAP7959120.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            6146..6349
                     /locus_tag="IVY70_001998"
     CDS             6146..6349
                     /locus_tag="IVY70_001998"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959121.1"
                     /translation="MSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNA
                     YIARLKRFIDKYKENSDKFSKDK"
     gene            6369..7997
                     /locus_tag="IVY70_001999"
     CDS             6369..7997
                     /locus_tag="IVY70_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959122.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            8048..8419
                     /locus_tag="IVY70_002000"
     CDS             8048..8419
                     /locus_tag="IVY70_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959123.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            8504..8704
                     /locus_tag="IVY70_002001"
     CDS             8504..8704
                     /locus_tag="IVY70_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959124.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            8838..10064
                     /locus_tag="IVY70_002002"
     CDS             8838..10064
                     /locus_tag="IVY70_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959125.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            10914..12239
                     /locus_tag="IVY70_002003"
     CDS             10914..12239
                     /locus_tag="IVY70_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP7959126.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRATDLPYPLPKELHIVPPRMNLYIKKNMEVNQI
                     FRKYVADEDLLIYSIDESILKVTKSLNLFTSSGTRSERRRELAKIIKDEIQKETGLIA
                     TVGIGDNPLLAKLALDNEAKSSKEFIAEWTYETVPEKIWKINEMTDFWGIGSRMKKRL
                     NHMGIMSIEDLANWNPYTIKSQLGVIGLQLYFHTNGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPKDYTRREEVELVVKEMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKI
                     PITDNTKELQNYCLFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEEN
                     LDRIIDKIRQKYGFTAIIHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            12232..12579
                     /locus_tag="IVY70_002004"
     CDS             12232..12579
                     /locus_tag="IVY70_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959127.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
BASE COUNT         4969 a         1588 c         2549 g         3686 t
ORIGIN      
        1 acaacaacga ttatgatctt aaaacaatcg tttatgtaat tggcaatctt acgaataatt
       61 ttcatgcaat aatgacaata aaaaatgaaa aggggtaaaa tcaaatgaaa aaatttttag
      121 taggaattct attcacaatg gggctagtat tattatcacc ttcagtttca gcagaaagct
      181 ctatttccat gtatcgtatg tataatccca atagcggaga gcatctatac actagaagcg
      241 ttggagaaag agacaatctg aaacgttatg gatggcatta cgaaggtgtc gcatggcaag
      301 ctccaacaag tggtactcct gtttatcgtt tgtataatcg gtataacggt gaacattttt
      361 acacagttga tgccggagaa agagataaga tttcacacac aggatggacg tacgaaggtg
      421 ttgcgttcta ttcctacaac ggagcaaata gagttcctgt aacacgttta tataacaaat
      481 acgtgaattg gcatcactac acgttagatc aaaatgaaaa gaacgtaatt agtaaacaag
      541 gttggaagat tgaaggtaca ggctggtatg caatgccaaa cagcgtacca aatcctacac
      601 ctgctccaag cactgaaaaa gtgttgaacg ttcctgttgt atggcaagga aatacaatgt
      661 tatgcgaagg agcttcttta ttagaaggtt tacactacaa aggtgtagcc acaaatcaaa
      721 gcttaaccag ttttgtaaac tatatgccaa aagctaatga caataatcct tatcatggtt
      781 actcaggaga gtggcgtcat aacgttaatg gaacgtatca aggcatgatg gctgacccag
      841 tagttaaatg ggcaaaaaac gttggcggta atgccgtcaa tatcactggt tcaggaacga
      901 atggaatcaa gaatgaaatt cgcaatggta atccggttgt aacttggatt acttataact
      961 atgctacgcc agagttcaaa caaatgcctt ggggtcgtgc agtatggaat ggacatgtag
     1021 ttctcgtgga tggcttcaga aatggtgcat accatgttgt tgaccctgta tttggcgcaa
     1081 aatggatcaa tgcaagcacg tatgagagag catttaatgt gactggaatg gcggttgctg
     1141 tacgttaatt gatagaatta tcaactaaat gaaataagag cttaattact gaaatggtaa
     1201 ataggctctt atttttttag aggaaatttt tgtaatacga atctataaca aaaaatattg
     1261 aatgtagtac acaaaagtgg tatattaaaa tcgtagagat tatgcgaaga ggtgattttg
     1321 atgaaccaaa gaagaagaaa aaggttagaa gaaataagcg agcgactttc cgagctaaag
     1381 gatgagttag aagtaattca agaagaagag tacaaagaaa atatacctaa aaatctacag
     1441 ggatcagaaa gatatgaaaa agcagaagaa tccgttgatc aacttgaaga agctgttgat
     1501 ggtttatccg acatattaga attgattgaa gatatagcta gctagagatg attaatgaag
     1561 atttcaggag tgagaataaa atgattgata tttcactgat agagaagcta cttttttcaa
     1621 ataagtacac agccaaagag ttggctacaa gatggcaaat atccgaatca gctattaaag
     1681 cctatcgtca aaataaggga agcgtaggat ggcgtgattg gaaaggaatc agcattggaa
     1741 aagcagaaga cataatgaaa cgtgaaggat ttcaagctga caatgattct aaaaaagttg
     1801 aatagtttaa tagtcgccaa gaaaagtacc aggacgaggg gaaaggtaga ttacaaagga
     1861 aaacactttt aaataaactt cgcaaaagca tcaggttatg agtggctagg gctatacgaa
     1921 tgtgatgaaa acaaagggct ttaatgtcaa agaaaacgca ataggggggg aacaaaattt
     1981 ttaaaaaaga tatttatgtt tggtttaatg gaaaaaaata tatgactact aacaaaaggt
     2041 ggtcagagct agtacaaaat acagttaaaa aagtcatgcc agatggatat catttcaact
     2101 atatacaatg ctcacaaaca actgaaagta tttatttttc aatattaaat gagaatagac
     2161 tctattattt cagggtaagt tcacaccata ggaaaaagag agaagataat gttacgacat
     2221 ttttatcggg aaaatacaat ggcttcaaag agttaagtga agccattggt gagtatctaa
     2281 aaagtaatag atttgtcaga atttctgaca aacatatatt gatcttgtta ctttatttga
     2341 ataatagctt caaattgttt tggagagacg acactttcta ctatattgag aacggcatgg
     2401 atatttattc tggtatcaac ggcatattaa aacattatat taaggcgtta cttcgatatg
     2461 gggttcttta tcaagaagat ttaagtaacc aaattaagat tagtaattct ggaaagtcat
     2521 tactggcaaa atataaaaaa ttgctttctc ctatctacaa aggaatagat atggaagctg
     2581 taaattctga aactttattt aatttagttg ttgatgaaaa gtttgcgaat gaatttttta
     2641 aaagtaaaaa aattgaaaga ctaaataacc tgtttcctga aaatgcaata aaagcttatg
     2701 tagataataa taaatcacga ttatacaact ggtttattcc caaacggttg cgaggaaaaa
     2761 tcaaaattgg cgatcaagtg aaagtaatta atctaaatca tactaagatc gttaccgtaa
     2821 cggagttgta tatagctgaa aaagaagata ttacaaaaat gaaaccagtt attaaaatac
     2881 tagataacaa agagtgagtg gaatatatgg tagcaaaaaa gaatactcta aaagaagctt
     2941 ctgaaaaata tcaagatatg aaaagacgag gattaatacc aaaggacaaa aaaggtatat
     3001 ggagagaatt tgttaaggaa gtcagtagtg atcgaagtgg cggtgacttt gttggacgat
     3061 atacagcaaa aggttatagc aggcgcttaa ggatcactgt tgaatatgga acgaatgaat
     3121 cagctacaga gctgaagcaa aggtacttag atacacgaag agaagcttca aaaaaatatg
     3181 atgatttcaa gagcagaggt ctaattccta agaagaaaaa ggggatttgg cgtgagtttt
     3241 tcaaagaaga agatagcaat cggaataagg gagacttcat tggacgatac acagcaaaag
     3301 gctacactaa gaatttaggg atcaaggttg aatacggaac tggtgaaagt tcatgggaat
     3361 tgagaaatcg ctatttaaat acattgttag agaaatataa agcagaatta ttggagttgt
     3421 tcgatgttaa tgattttatc gagatggctg aaaagtatgg tcatgaagaa ggttttatga
     3481 tcaatgacat tatggttatt gataatttta cggatttatt agcacttgcg aaaaaagcaa
     3541 gggatttaag agaaacgcag atgtatctat tcagaaataa aaacggaaga aaccggagaa
     3601 ctgaatcgag tgagattgaa gattactatg atgcctttac acacgatctg ggagcctttg
     3661 tagacgagat aagtggtgta atgaacaaat atagggtcta gaataaaaaa gccggagatg
     3721 agcagataaa tgggaaacaa tagcatatac acacctgaaa tgcagaataa aattaaaaga
     3781 cttgtcggat tagggtggat gacaagagga aaattagatt cttttagtac gatcaaatat
     3841 cttttgatgg acatttaccg tcagaacaag caaacaggaa tacatttttt gggctatgat
     3901 aagccggaag atgcaccttt ctttgttcca agttcagcct atagagccag tacctccggt
     3961 agcatgtata acttggattt gtttgaggaa ggagcaaaga tgatcgaata tgatttcaac
     4021 gaagcctatt cgaggatcat gagaaattat cctttgccgt caaacactta tttgaaagca
     4081 aaatataata cagatcaagc tattttgaga gcgataaata gaccaaaagt aagaccgtat
     4141 gaaaatttaa aaacgtatat gttctttttg gttaattttg aaggaacagc caaagaagga
     4201 acatatgcta atccaaaatc acagtcgatg tttgcggatt atggcgaaaa tcccaaaaca
     4261 gctaatgagc ctatttggtt atcggagatt gagctaacac ttatccttga ttattatgat
     4321 attaaagaga tggaagtcct agatagccac acatttttga cgaaaaaagg attactcaaa
     4381 gaatattttg aaagaatcga gccattaaaa actgatcctg atacgttgaa gctttataaa
     4441 aatcttagaa cgaaagtatt tggtgctatc ggacaaaaat cattaagaga aggagaaaca
     4501 agcaccttcg ataaatacag tatgtataat cgagcgtatt cctccatggt agcaggcgta
     4561 tttagagatc gtatggttag atatgaacag aaatacgttc atagcgcata tgggctatta
     4621 aaaattcgta ctgatggatt gtatttcaga tccgaagtac cagagtttga agagctgtat
     4681 aaacttggag tagttaagaa aaaagttcat acaatatcca aagaagaagt cgaaaaagct
     4741 ttagaattta aaaacaaagg atttgaaaat aagggctcct ttgggaaatc taaagatgaa
     4801 gttaagggtg ttgtgataga cgatgatttc gagtttgatt ttgactaaat actctttgga
     4861 aaagatggta ggaggtaaat atgaagaaga aaattgtgga aggaaatgta ggtgttatta
     4921 ttctaaaaga ttcaactgac tatgctaaat caatggcact aaaagacgaa aaagttgcac
     4981 gcatggataa agatttagtt gaatttctga tggatgatac agaaagatta aaaggggatc
     5041 agtttttgag tttaatgaaa gacaaaggct taaacacata tggtttattt gattataaga
     5101 aattagctat agagtgggta aaagaaggag aaatattttt tattgaagaa tgggaaggta
     5161 ctgagaactt agttcttcat cagtatcttt tgaaagctta atctaagtaa aacagttctg
     5221 actagttatg cttgtcagaa tttcaaagga agttttccat aaccatcatc attcaaaaat
     5281 aaaggatttg aaaataaggg ttcctttggg aaatctaaag atgaagttaa gggtgttgtg
     5341 atagacgatg atttcaagtt tgattttgac tagatttttt tgcaatgttt aagagtaaac
     5401 aaggtaagaa ctaagatcat gaagattgag agtaaagaag atagaggacg aatgtatgag
     5461 aacacactat atttatttag aagataaaat tgtagatgaa attccggcaa aaagtcgaga
     5521 acaagctaga aaattattta ttgaaaaata caaagagatc aatggagata gtgaagttcc
     5581 aaaagtggtc gtaacagtta gaaaaaggca aacaaaaaag agaagtacgt ttcatgtcag
     5641 ccctgatata tataatctta tagagcgaca tagaagtgaa gataatattt ctatctctca
     5701 atatattgct acagttattg atgaattgat ttcaggaaag tatgactatg cttttgattt
     5761 tgagggtgaa aaggattaca aaagtgttgc tgtctttcaa tctgattttg agaagctaaa
     5821 aaactatgct ggcaggagaa atgttaattt tgcaagacta ctagaagagt tctactccga
     5881 agagttggga gtaggaaatg aatacaagag agaaacttat gcgaaattgt ctcataagtt
     5941 atcccaagcg cttaaagaag tgacagaagt taaagcggaa ctagatgcta aacttgaaga
     6001 agtgaaaaaa tatcaaacag agaggaagaa caacatgaag aatcaagtgg aatttgacga
     6061 acagaaagaa tttgaaaaag caagaaaacg ctataacaaa attatgaatg aacttagcta
     6121 tgaacactta acagttcatg aataattgag tgagggtaca gaaaaatata cgatcgcaga
     6181 tatggttaat gaagtggaaa ctcttttagc ttctttcttt gaaaatggtc acgtaaacga
     6241 tgaatggcga aaagaagaac ctaaaagggt taacgcttat attgctcgat taaaaaggtt
     6301 tattgataaa tataaggaga atagtgacaa attttctaaa gataaataag gagagagtgt
     6361 gattacattt gccagaaatc gaaggaatat tttcgccaaa aataggtaaa aaacagttag
     6421 atttcagcag ttttccgagc gtaaatagag attataatct aggtgatgag gtacagatac
     6481 taaatattga tttcaaagaa ctagacttcg ctgttgctgt ttccaacaaa cccaaggcat
     6541 tggaacgagt gaaaaaaata taccaagaga atgatcaaaa tatcaaaaat atgatcaaaa
     6601 agtatagacg agataattca aaagttgaag acgttttatt caacggtacg gcaaaacatg
     6661 atttgaatgt ttatatggca ttaggagtgg ctttgtactg taattcaatt aatcagacag
     6721 atattttggt taatatcgtg aggaatagct atcaaaaaat attaaaaata agtagcgaaa
     6781 caaaccctta ttatgcaatg aaatatcttg gggataattt attaaatgat aaaaaattga
     6841 tgcacaacac tgaagagctg ttatctaatt tggctgcgat taactttcta ttcaaaacag
     6901 gttctgaaga ttattttata gataacgtac taaaaggtgc ttacggaaca gcattttatc
     6961 cgctttacaa tatgaaacag cataaagccg aacaaaagga taacaaactt gaaattaaaa
     7021 agatgaaaga actattaggt ataggatttg taaaacccaa ttcaaaagtc actattgctt
     7081 cgttagcaac tactaacaat gatgggaaaa tagtaaatgg gtttgacgat tcagatatag
     7141 atatattgtc aaaatcatat ttttctttgt atggagattt tgcagatttg atgggtattg
     7201 atagttactt agtcaataat tttgagatca agggtagcga gctgaatgat attgtgtatt
     7261 tcgttttaat ctctctatat gcaattgatc agaataccga gtaccgacaa attgactatt
     7321 tgctagtttt ttatagtttg ttatatcagt tcgttttaac tcggttgttt aaaaatgcta
     7381 aggaagaatt gatcctgaat tctgattatg aaatagcaaa acaaattgca agtattagaa
     7441 aagaaaacaa atctgaagta atcaaattaa gaaaagaatt aagtattgcg agtaaagaaa
     7501 acggtcggtt agaatcttta gtcaaatcgg caaaaaaaga agaggaactt ttgaagcaag
     7561 agattaaaga attaaaagga atcagaaaag aacttgaaca gctgaggttt gaaaataaag
     7621 tattaaagca attgcaggag caagaacata gacaatcatt aaatggtaaa actgaagagc
     7681 cttcactgtc tgaaatgata gcttatctaa aacataaaaa cattctttta gtgggaggac
     7741 atgtaaattt tcaaaataaa ttaactgaac tattaccaaa tattaaaatg tttgatgtaa
     7801 gcgaaaataa tggaaagata cttaaaaatt tgaatacgtg tgatgcgatt tttctatata
     7861 cagattactt gaatcatggt ctatattatc gtgttatgag tgaaatttct aataaagata
     7921 agatatttta tttggataat catgtgaata tgaacttaac aattaaacgt atttacgatc
     7981 ttttatcact tatataaaga gagaagtgat aaataggaca caaaatttgg aaagtaggta
     8041 agtcactatg agagaactaa ctttatcttc taaggaatta gaaaagaaaa ctgctttatt
     8101 aaacgaaata tttagtgctg attgtataat ccaaagttta aaagaaagtt cattttcaaa
     8161 actaacagat caggaaaaaa tttcttatat gctaagttta gataatctag gaactaaata
     8221 tgatgaatca gcaagtagcg ataagctttt gattgaatta gtatcaagtc ttagtagttt
     8281 tggaaaaatg aaaagattta atgtgatatt agaaattttt gcttggttaa tcccttcatt
     8341 tatttggttt ttttatattt tcatagttga atctgagtta atagccaagg tctctcttgg
     8401 actgttaatt ccgatataaa taggttcagc aatttacttt aataacttgt atacacgtat
     8461 tcaaataaaa aagcagttat tacatgaagg attaagtggt aatatgttaa aaagaattgc
     8521 atgggataaa gataataaaa cagatattga gcttaaagaa tataaataca attatttctc
     8581 gagatgtata gatagaaagt tactgtttaa aaaagaagga ttagaattta gttttgatga
     8641 agatgaattt gaaaaattag tgatggactg gcgtaagaat ttactggata cggtacaatt
     8701 ctaaaagcaa ttgaggtcga aattccgata cttgaatcat gatctatatt atcgtgttat
     8761 gagtgaaatt tctaataaag ataagatatt tcatttggac agtcatgtga atatgagctt
     8821 aacaattaaa cgtatttatg atcttttata tgtttataaa cattaatgag gtgctaaaat
     8881 tgacaacaat aactatgaca aaaaaaatag aagtatatgt tgatggatca caagaagaaa
     8941 gggattcctt ttttgatttt ttttggaaaa atagtagaga atatgtaaaa gcgttgaatc
     9001 tggtatataa acagctaatg tttgaagagt tgataaaaga tgcaattaaa gaaatggatc
     9061 aaggttatgc tgagagagaa gaaagtattc aaaatcggat tgaaaagtta gcatatactg
     9121 atccaataga tcaaaaaaaa cttgataaag caaaagctga attatctaaa ttgagaaatg
     9181 aaaaaaataa agaagcgaga gaaatattaa cgcaagcgat agggttgaag agtcaaacga
     9241 gaacacgaga tgtagcaaaa aaattagact ttgactttgg agattttgtt gattccgcaa
     9301 atatgaaagc tagtaaagat tttggcaatg attttatcgg aattgtgacc ggacaaagaa
     9361 cgcctagaat atataaaaat agaaacaaat atggattact accttttaga ggtcgagata
     9421 ttaaaatcca taaagatgat aagttttata tttatttacc taaaggcttt aggtttaaag
     9481 tgaatttagg tagcaagcca agaaaagcaa gagatgttgc ttcagtttta caacacatta
     9541 tggatggcga ttacaaatta aatcaaagta atcttatttt tgatggaaga aaattatttt
     9601 ttaatctaac aatttctttt gaaaaaagct ataatgaaat ggaattcaag aaaggtaatg
     9661 aattgatcgt aaactttgca gatttgtgta gcggtgaatg tttaattaat gataaatctg
     9721 taatgtcttt tggtgatagc caatatataa gaaacttcaa aagtaaaatt ttagcaatga
     9781 aatcaaaaga acaaagccga ggagtctttt ctaagggcgg tcatggtcga aaaagaaaat
     9841 tagatcaaga tcgatgggaa gcaataaaga aaagagaaag taattttgtt aaaacttata
     9901 ataatcaatg ttctgcaaaa atcgttaaaa ctgctattcg caataaagct caaattattc
     9961 gtatcattga accagataag gcttatggcg aatgggctta ctatcaattg aaagaacaac
    10021 tagtaaacaa aggaaaaaga tatgggatag aagtcagaag ttagtaaatt aaacaatcgt
    10081 tgatttggca acgaaatagt gagggaaaat ccactcactg aagggtaaac tccgtcaagt
    10141 tgtgtgacca gacggaagga cgaatgttgt tgaggtttta gaaccatata aaacagcatg
    10201 tttctcaaat ttttaggata agggaaattg ctagcagttt gttttagaac catataaaac
    10261 agcatgtttc tcaaatgagt actttaaatt aattgatggc gttcctgctt tagaaccata
    10321 taaaacaaca tggatttaaa acaagaagta agtcagttct ccttttactt tagtaaggaa
    10381 aaataaagta ttctcaataa aacttaaaat aagcaaacct ttgttttaat agctaatcga
    10441 agtaaaaccc aaagccatat gaacccctga atcagcgaaa gtttatgatg atttggatta
    10501 ctaatagttt aggaattaga tggtaaatct aaattaaaca atcgttgatt tggcaacgaa
    10561 atagtgaggg aaaatccact cactgaaggg taaactccgt caagttatgt gaccagacgg
    10621 aaggacgaat gttgttgagg ttttagaacc atataaaaca gcatagttct caaattgttt
    10681 aaaatgattg tcaagttaaa aaaatctggg atcatataaa atagtatagt tctcaaatat
    10741 gattagaaac cctattaaag agcaatatta agccaactta cttaaatcgt ttaattaaga
    10801 acaataatct cttttagtca gacttttagt ctgacttttt tgtatttcca aaatttattc
    10861 tttacaaaaa cgaacatacg tttgtataat tgcttgtgaa tgggggaata aaaatgtttg
    10921 attacagtag agaaccggtg aatgactatt ttttaatcga catgaagtct ttctatgcta
    10981 gcgtggaatg tattgaacga aatttagacc caatgactac accgttaatt gtcatgagta
    11041 gatcggacaa cactggttca ggtctgactc tagcttcttc acctactgca aagaaaaaat
    11101 atcatattac taatgttagt agagctactg atttacctta tccattacca aaagaacttc
    11161 atattgttcc accacgaatg aacttatata taaaaaaaaa tatggaagtt aatcagattt
    11221 ttagaaagta tgtcgcagat gaagatttat tgatttattc gattgatgaa tcaattttaa
    11281 aagtgacaaa atccttaaat ttatttacta gttcaggaac aagaagcgag agaagaagag
    11341 aattagctaa aataatcaaa gatgagattc agaaagaaac aggtctaata gcaacagtcg
    11401 gaataggaga taacccatta ttagctaaac ttgcactgga caatgaagcg aagagtagta
    11461 aagaatttat agctgagtgg acttatgaga ctgtacctga aaaaatatgg aaaataaacg
    11521 agatgactga cttttggggc attggatcac gcatgaaaaa aagattgaat catatgggaa
    11581 ttatgagtat agaagattta gcaaattgga atccctacac aattaaaagt cagttaggtg
    11641 taatcggact ccagttatat ttccatacca atggaattga tcgtacggat attgctattc
    11701 caccagaacc aaccaaagaa aagtcttatg ggaattcaca agttctacca aaagattata
    11761 cgaggagaga agaagtagaa ctagtagtca aggagatggc agagcaagta gccattcgaa
    11821 ttagacaaca caattgcaaa actggttgtg tacgcttagc aattggtacg tcaattttgg
    11881 aagaaaaatc aggtttctcg caccagatga aaataccaat tacagataat acgaaagaat
    11941 tacaaaatta ttgtcttttt ttattcaata agttttatga ggggcaggaa gtacgacata
    12001 taggagtaac ttatagcaga ttagtataca cagattccct acaattagat ttatttagtg
    12061 atccaagaaa aaagattgct gaagaaaatt tggataggat tattgataag attagacaaa
    12121 aatatggatt tacagctatt atacatgctt catctatgtt agaaggtgca agaagtatta
    12181 cgagaagcac tttaatagga ggacacgcag gaggattagg aggtttgcat aatgattgaa
    12241 tataacgata gaggacgttt aaaatggtcg ggattttatt tgtctgatca tactgaaaga
    12301 atcaatgcag acaatgtaca acgtgctaat cataatttgg caaaagaaca aatgacgact
    12361 gaagagattg cgaaaatact gagaactgct gtcataaaaa atagacccgt ctgcattcaa
    12421 aaagaggaac gaaacgctga aggttactat ccagatgatg tagtaggaaa aattattgga
    12481 tatgacgagc ttggtgtatt tgtggaagaa gaaaaagttc actatgatga aattaggaat
    12541 gtttctttct cggaattatt aaaatggagt cagatatgaa cattattaaa aaatacgaat
    12601 tcggttatat tacgtatgaa cagctaatag aggaattatg gggatatggt caacaattaa
    12661 ttaatgaagt ggggattgat tgcttttgtt tctatattga atccggagca ggataccata
    12721 agaacagata ctatatattt ccatatccac caaaatagtc tcttagatat ttttaaattt
    12781 tctccaaata ct
//
LOCUS       DACCXO010000079        12737 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.080, whole genome shotgun sequence.
ACCESSION   DACCXO010000079 DACCXO010000000
VERSION     DACCXO010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.080"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..1144)
                     /locus_tag="IVY70_002005"
     CDS             complement(<1..1144)
                     /locus_tag="IVY70_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP7959128.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLL"
     gene            complement(1266..1862)
                     /locus_tag="IVY70_002006"
     CDS             complement(1266..1862)
                     /locus_tag="IVY70_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7959129.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVDLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(2062..4704)
                     /gene="alaS"
                     /locus_tag="IVY70_002007"
     CDS             complement(2062..4704)
                     /gene="alaS"
                     /locus_tag="IVY70_002007"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP7959130.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            5092..5523
                     /locus_tag="IVY70_002008"
     CDS             5092..5523
                     /locus_tag="IVY70_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7959131.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(5568..6917)
                     /locus_tag="IVY70_002009"
     CDS             complement(5568..6917)
                     /locus_tag="IVY70_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP7959132.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(6987..8150)
                     /locus_tag="IVY70_002010"
     CDS             complement(6987..8150)
                     /locus_tag="IVY70_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959133.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            8279..8602
                     /locus_tag="IVY70_002011"
     CDS             8279..8602
                     /locus_tag="IVY70_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959134.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(8785..10134)
                     /locus_tag="IVY70_002012"
     CDS             complement(8785..10134)
                     /locus_tag="IVY70_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7959135.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(10203..11156)
                     /locus_tag="IVY70_002013"
     CDS             complement(10203..11156)
                     /locus_tag="IVY70_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP7959136.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(11258..12448)
                     /locus_tag="IVY70_002014"
     CDS             complement(11258..12448)
                     /locus_tag="IVY70_002014"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP7959137.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         3653 a         2686 c         2210 g         4188 t
ORIGIN      
        1 ataaaagata attgtgatca aacgtaattt ccaattggta atctccgatt ttttcttcta
       61 tttgactacg taagattctt tctagttctg tatcaacaaa ctggaatttc tcagggacca
      121 ctacatcaaa ttgcagcgtt tttccttgat gaattactct aaaatcatga atattaagcc
      181 ctgtacctgt ttcaagaaga atttgcttta actgccgcaa gatattatag tatttttcgt
      241 tatcaacagg cattggatcc aagtgacaga caagttccac atccaaagac tttttaaagt
      301 ctttttctat cagatcaata atcttatgag ccacattcaa attcattgat tcatctactt
      361 cgatgtggac tgatgcaaag cgtttattgg gtccatagtt atgcaccaat aaatcatgat
      421 agccaagtat atcatcgcaa gcatttaaat gctcttccat ttcttcaatc tctgctggcg
      481 ttgggcgact tcctaatagt tcgtatacaa aatttcgaag catcgtcaat ccactataaa
      541 ggatatataa tgctaaagca aaacctatat atccatcaat acgccatcca gttacccatt
      601 cgattgctgc tgaaagcaat acagccagtg tagtaaagac atcattataa ctatctgttg
      661 ctgtagctct caatgtttca gaatcaatgg attgcgcaat aacctgatac attttgcctt
      721 gtgcaaattt caaaaagatg gaaatgatta ggacaaaaaa gacgatgggt gttaaagaga
      781 cattttctgg atggatgatt ttttcagcag aagactctaa aaattgaaat cctacataag
      841 taatgatgat cgaaacaaat aacccactga tatattcgaa gcgttcgtgt ccataaggat
      901 gttcctgatc tgctggcttt gcagcaattt taaatccgat caaagtcaat atggatgaag
      961 cagtatctga tagactgttc actgcatcag ccataatgga gacacttcct gaaaaaagcc
     1021 cgatcattag cttagagaca aacaagatca gattcgtaac caatcctaag atacccgcta
     1081 gtactccttt ttttgtccgc tgttgcggta tttgtttctt tttttcttct tgtttcttag
     1141 ccaagttttt taccccttct agtttgcttg ttgctcaggt aaaaagatgg agaggacgat
     1201 tgttttcctc tcctctcttc ctttttctat ctatcgtgcg tcctattact attaaaagat
     1261 ggtcattatt ctggtttcaa cggtgttgct gcaaataacg cttttgctag taatggtgtt
     1321 atgattcccg cacctattgc ttctggtatc ccattgattg ctgcaacccc tgctaaggtt
     1381 ttaaataaaa gagcagagtc tacaccatag gcatctgccg ttgcacctgt ataaaataat
     1441 cccatgaatc ctaacactaa gactgtattg accaatgaac ccagtacccc tgcaaaggct
     1501 gagctgatcg ttattgattt atttttttga taaatccagt ggaaaaccaa gcctgctact
     1561 aagtcaacaa gtactctggg aactaccgag ataatcggat tcgtgaacac taatgtatca
     1621 aatggcgttg ttggcatggc ataggcacga acaattgtag cgattcccca aaacaagcca
     1681 atcaccatgc catcttttgg gcctaatact actgcagcaa taattactgt gatatggata
     1741 attgttaaac taatgaatcc taatggaata aaccccagcc aaggaaccat cgcttgtacg
     1801 atgatgattg ctaacaagat cgcacgtaag actagtcgaa aggtcttatt tctactattc
     1861 attttttcac ccctttttat ttatactaat cgtaaaaatt tctgtcactt tcgtcaacta
     1921 aagaccatcc gaatccgacc atttgattag atgcgccatc caattgaaaa aagtatgagg
     1981 gcaagacttg tttgtcttcg cccccataat catgtttcgt tcaatgtttg aacttttgtt
     2041 tttctgtcaa acgtaaacgc cctattgttg cgcaacccat tctttcgcag ctttaagtgc
     2101 atctggaata ccagcaggat ttttcccgcc agcttgtgcc atgtctggac gtccaccacc
     2161 gccaccgcct actttcggtg cgattgcttt aatcaagtcg ccagctttta gtccagcttc
     2221 atttgattct ttcgtcattg ccgctagcag actgactttt ccttcttgtt cgtttgctaa
     2281 aacaagaata tctgaaatat ttttttgttt ccattggtca gctaattgac gcagctgatt
     2341 catatctttc acttttactt gtgctgcaat gattgttttt ccattgatcg tttctggatc
     2401 tttgaataca tcaccagctt gttgattcgc taacttgcta gctaattgtt cattttcctt
     2461 ttgtagctct cttacttgct gttgcaattg ttccgctttt gaaacaactt ctttcaattg
     2521 aggtgattta acaattgttg ctacttgttt tagttgtttt tcttcttctt caagtagttc
     2581 gtacgcttct ttacttgtta cagcctcgat tcgtcgcacg cctgcaccaa ttcctgattc
     2641 agaaacgatt ttgaagattc cgatgttttc tgtatttgct acatgtgtgc ctccgcacag
     2701 ttcgattgac cagccgccaa tatttactac tcgaacgtca tgtccgtatt tttcaccaaa
     2761 caaagccatt gcacccatgt ttttggctgt atcgatatct gtttcaattg tttcaactgg
     2821 gatagcttcc cagatttttt cgttgacgat tctttccatt tcagctaatt catctgaagt
     2881 gatttgacca aagtgggtaa agtcgaatcg taaatgtcca ggagcgacta aagaaccagc
     2941 ttgatttgca tgctcgccaa gtacttcttt caatgccttg tgcagtagat gggttgctgt
     3001 atggtttttg atgatccgat tgcgcatcga ttgatcgatc aatagatgat aggtttgtcc
     3061 ttcactcatt ggtgctttta cttctacggt gtgcataaat tgaccattag gcgctttttg
     3121 aacatctgta acaactgcta cgatttcgcc tacttcattc atgatccatc cttgatccgc
     3181 tacttgccca cccatttctg catagaaagg tgtttgatca aagatcattt gagcttcacc
     3241 ttctgcgact tcattcaaaa gagactcacc tttaacgatg accaataatt gactatctga
     3301 ttcttgttct gtgtatccaa tgaatttact ttctactttg atatctgtca ataaagcaga
     3361 ttgtaccccc attgattttt cagtactgcg ggcagagcga gcacgttcgc gttgtgcatc
     3421 catttctttt tcaaaaccag catgatcaac tttcagtcct tcatcttctg ccacctcttc
     3481 cgttaattct accgggaagc catatgtgtc gtatagtttg aagatgtctt ttcctgctag
     3541 agtcgtcccg ttttcgtttt ttacttcttg gatcacttga tttaagattt ccaaaccttc
     3601 attgattgtt tcattgaaac gttcttcttc tgtacggata actttttcaa taaattcttt
     3661 ttgttgaagt acttcaggat aataacttac catgatttcc ccaacaacag ggactaaacg
     3721 atataagaat gcttgttcaa tacctaattt tttcccatgc atgacagcac gacgaagcaa
     3781 acggcgtaag acataccctc tcccttcatt cgatggcaat gcaccgtcgc caatagcaaa
     3841 tgaaagggcg cgaatatgat ctgcgattac tttgtaagaa atatctgtta ctgggttttc
     3901 accatatttt acttgtttgc ttagcttttc tacggcgtgg ataataggca taaacaagtc
     3961 tgtctcaaag ttcgttgacg catcttgaac gattgagacc atgcgttcca atcccatccc
     4021 cgtatcaatg tttttatgag gaagtggttc aaatgtgtta tcaggcttat ggttgaattc
     4081 tgaaaatact aagttccaga tttctaaata acgttcattt tctcctcctg gatagttttc
     4141 aggatcgtct tctgctaaat cgttataagc ttctccacgg tcatagaaaa tttccgtatc
     4201 cggaccacat gggcctgcac cgatatccca gaagttgtct tctacatcaa taatgtgatc
     4261 tagtggcagg ccaacttctt catgccagat gcgttttgct tctgtatctt ttggataaac
     4321 tgttacataa agtttttctg gatcgaatgc catccatttt ggatcagtca aaaattccca
     4381 tgcccagtgg atggcttcat ttttgaaata gtcaccaatc gaaaaattac ccagcatctc
     4441 aaacatcgta tggtgccgtg ctgttttacc gacattttcg atatcatttg ttcgaataga
     4501 tttttgggca tttgtgattc ttggattttc tggcacaact gaaccatcaa aatatttttt
     4561 caatgttgca acaccagagt tgatccacaa taaggttgga tcgttcacgg ggatcaaaga
     4621 cgcactaggt tcaatcgtat gtcctttaga ttggaagaaa tcaagaaaca tttgtcgaac
     4681 ttgtgcacta cttaattgtt tcatgtcatt acctgctttc tataaattaa atacaaaaaa
     4741 acaccatcac agccaaggac gaaaactcgc ggtaccacct tgattgcaat gatgtgtaat
     4801 cattacctct taaactcgat aacgctgagc tgcgtcggta tttctaccaa aatagaaaaa
     4861 gggagcaatt tgattagtga ctattctttt ttcagccaag aaagaatttt ctgtgagtct
     4921 taaaaaacaa atcatatcct tttcgtactt atttagtata cgcattctat tgatcactgt
     4981 caatatctca atttttattt tcatttcctt ttggtcatac tccacgattt ttggtacgat
     5041 aagaataaag gaatcaatac atctctgcta ttcatgacaa ggaggaatat gatgaaaaaa
     5101 atagttggag cggatgaatg gatacgcgca gctagctgct ttttgagata tcaagttttt
     5161 gtattagaac aagggattcc tgcagaacta gaattcgacg gaaaagatat cccgcaacaa
     5221 acctattatc tatttcttga tcaaaaaaga ccagtagcta ctttgcgtta tgaaaagaaa
     5281 acacctactt gcttcaagcc ggaacgattt tgcgtagcaa aagaataccg aggaaaaggg
     5341 ttaggcggtc agttgcttga agctgctgaa aaacgggcgg tttcagaagg ttgtcagact
     5401 agttatgtag tagctgaaat gacggctgta gatttttatg aaaaacaagg gtatcatact
     5461 tgcacagaac cttttattga agatgaagta ctttgtatcg gaatgaaaaa aagtctagct
     5521 taaaaacaag agattgtgac acgccttttg tcacaacctc ttgtttttta tttatgtgaa
     5581 tttttcttat ttttacgagc agtacggctt tgctgtctgc gttcgatttt gcgttctttt
     5641 ttattttttt ctgcaattgc ccaatcgatt tttttcttat aaccaggctt gatctttttc
     5701 tttttcttct tcacaagacc aatcagcgtg ggatctaata catcttttga tttttctcgt
     5761 ttggcacgtc tattacgatc gtatgttttg acgatttccc cgtttttgat ttcttttggt
     5821 tcaaatgaga cgcccaattt ttctatcgca tcaatggctt gctcatcatt tggcgaatat
     5881 aacgtaatgg ccgttccttt taagccatta cgtccagtac gtccgacacg atggataaag
     5941 aagtctagat cttctggaat ttctgcatta ataacatgag agacaccttc gatatcgatc
     6001 cctctagcag ctaaatcagt cgctacaaca tattgatact ccagattctg tacttgtcgc
     6061 atcactcgtt ttctctctct tggggtgatg tctccatgaa tctttgctac tttcaagcct
     6121 tggtttttca agtaatcggc aatttcatcc acatgctgtt ttgtattggc aaatacgatt
     6181 gctaaataag gatgaccaat tgtcagcagt tgataaatca tttgattctc atctttacct
     6241 tttgttgaaa tcagccaatt ttcaatgtct tcagaaataa ctgcttttgg tttgatttct
     6301 tcaatgattg gattttccat gtattttcgt aagaatggac gaagtttttc tggaatagtg
     6361 gcagaaaaaa ctaaaaactg cagcttttct ggtaatcgac ttgcaatttg gtctacttca
     6421 tttaaaaaac ccatatccaa tgtcatatcc gcttcatcga caacaaatgc aaatgcggtg
     6481 tgaatcttca gcgcttgttc attcatcatg tctaatattc taccaggcgt gcctactaca
     6541 acctgtggct gttggtgatg cagacgattt aattgacgct gtttgtctgt accgccgaca
     6601 aaattcgaga cacggatctc cggtgtagaa aatttagcga tttgtgtagc tgcttcatag
     6661 atctgtgcag ctagttccct acttggagct gtaataacaa cttgtacttc ctctttttct
     6721 gcattgattt tttcaaacaa aggcaacaaa aacgtatgtg tttttcctgt accagtttga
     6781 gattgcccaa tgatattttt tccttttgca attaaaggaa tcaaacgttc ctggacctct
     6841 gtgggctgtt tgaagccctt tgcggttaac gcttcattga taaacggttt gaagcggaat
     6901 atatcaaaat tattcattcg tgcacctctt ctctttaaat tatcacgact ttggcattat
     6961 aacacagaca aagcgttttt gctcagctaa tttcgagaat catcatcctt tgtttggcca
     7021 atcgtgctaa cagtatatgt gtgactagct ttttcataaa acagaatccg tttttttctc
     7081 aaaaatgata gcaagatatt taaaataaaa ataccttgta gtaacaagac accgagagaa
     7141 atgaatactg cactcatctg caacaccgta ttcgttgtcg aattcatgat ttgctgaagc
     7201 aactgcccca accagcttaa accataaaaa agaaagtaca gatacccgta gcggatcatc
     7261 aatgctttta aagaaattcg ttcgcggtct tcttccacca aacggatacg cacaattttt
     7321 ttgccaagtg tccatccatt ggttataaac ggtacaatca taaagtatac ccctacttct
     7381 aataaaagcc accagatatg agaagtaact gcccctgaat cgaacgcaat tttaaagaaa
     7441 atcgccgcta ccaaattaat aagagataaa aatgtccagt cgatcaaata agcgaccaag
     7501 cgtcgagtca atgagacagt ctttcctttt tcataagaaa gttcatccat tcgtcttctt
     7561 gtaggaagca taaatgtaaa taacggcgta acgacaaaac caatgattcc acccaatgta
     7621 tttgtcacta aatcattcac atcaaataag cggtatggtc ttgggtagat aaaataaagt
     7681 ccacttagct gcgtcaattc aaaaaataga gaaaggcaga aactagcaat catcgttttc
     7741 ataaaagaaa gccggaaata atatcttaaa taaatgccaa atggtatgag taataaaaca
     7801 ttaaaagctg gttccaaaaa tacactctgc ttcattgccg gcaggtaggt actaggttca
     7861 gacaagacca ataccgtgtt gtctctgaaa tgttgccatg aagcaaataa atgcagctcc
     7921 atctttggcc ctgtcatttg ggcaaccgtc tcctttgccg gaagtggtaa gatcaccaaa
     7981 aaatacgcac acaataaata aaaaacgaaa gaatacaaaa taactgccct tgttactaga
     8041 aatgttccat actttcgata ttcataaata aaaaaaacag atgagatcgc taaggctaaa
     8101 aatggaaaaa caatcagcgc aaaacgaatc ggttcgatat aacttgccat atgactcctc
     8161 cttgattctt tgtatctctc ttatcttagc agtcctttgc tgtttcaagc aagtaacgag
     8221 gctattattt ctcatttctt tcaaagtaaa gtaagataaa cacaaagggg gtgtcgagat
     8281 gctgaatatg tactttgttt ttggcgtacc tattttttta ctgttccttt atgctacgat
     8341 cgcttatgta agaaaaagaa ctacaattca ttatttagga tttattctcc tgattatttc
     8401 gggtttcatg ctagtcttca atcttcagac ttggcagcaa gcacttcttg aaatggataa
     8461 aatgactcca catgctttgt ctaaagtgct tggctaccct gtctatctga tttggcttcc
     8521 cattttcatt tctggttgct tggttttatt aaatatttat cgcggcgtac gccgaatagt
     8581 tcagttacgc aaatcaaagt aaaagacaga aatcggttga gcccgatttc tgtcttttac
     8641 tttgctattt catcgtagac aaaacgacat ttacgtgatc aaaattaacg agtacttttg
     8701 acatttcttc cacgtactcc tcatttaaat aaaatatttt gtttggcaga gaagtgcagt
     8761 tctaataaaa ttcgaatgtt ttagctatct acgttttaaa tagagataaa tcgggatacc
     8821 agcaagtgtc aatactatcc ccatcgaagc taaaaaggtc tgagtgataa gcgtgcttga
     8881 tacgataaaa gtccctccaa tgattgctac tagtggaata aacggataca ttggaacttt
     8941 gtatggtcga aacaaatcag gttctttttt acgaagaaga atgacaccaa caaagaccat
     9001 cgtataaaaa atccaaatga caaagatcag catatccgtt agtgtatcaa aaccaccgat
     9061 catcatcatg ccaatagcga tgactagctc taagatacct gcggctacag gaactttagt
     9121 tttatcatga agtttagcaa agagcttgct aaaaggcagg ttgttttctt ttgccataac
     9181 ataagggaga cgcattccag taagggtata gccattgatg gttccataaa cagaaatcaa
     9241 gataccaact gtgaccagtc ttccaccgaa gccgccaaaa atcatttttg caacgtcact
     9301 tgccgcattg ctgtttcctg cgactccgtc gatagatgcg gttcttaaga acaccgcatt
     9361 tactaataaa taaacgatca tgattccgat gattcctaaa gaaattgctt ttggcaaatc
     9421 tttcgccggc tttttcaatt ctcccgaaat atttcctacg tgtatccaac cgtcgtatgc
     9481 gaacatcgtt gctagcaagc ccgctcccaa ggctgagaaa aatgaaagat tttctcctgc
     9541 ttggatcgga aacagttgga agtctactcc ttcttgtcgg aacaaaccga aaatgacgat
     9601 cacaaacaaa ggaataagct tgcagacaag cgtaatcgac tgaaaagccc ctccagcttt
     9661 agaacctaaa aaattgatca acaggatcga tacagcagct gtaacagcta cgggaacaat
     9721 cattgactgg gaaagaccaa atagattgac gaactgtgta ccgaaaatga tcgacaaggc
     9781 tgctacattt gctggaaaat aaatcacgac ttgcgcccag cccaataaaa aagctgctgt
     9841 atttccgtaa atccgttcga tatatttaat catccctcct gtttctggaa tagcagctgc
     9901 cagttcagct cctgttagtc ctgcacagac agaaatcata ccaccgagaa accaagaaaa
     9961 catatgcaaa ctggcggatc ctgtaacttc tgctacgctt gcggctttaa aaaatacccc
    10021 tgcaccgatc actgtgccca taacagtcga taaagcagta aaaaatccca tcgtacgttt
    10081 taattcattt tctattttta cttcttttac tggcgtggct tgatattctt ccatcccttg
    10141 tcccctttca taccatagcc aatcccaaaa gtataaaaaa ctttttggat gtggttcttg
    10201 ttttataccg tctctaccaa tttttctttc agtttttcca acatatcctt tgtcatcaca
    10261 tctaagtcgt atttcggcga aaagccccat tcctcttgcg cacaagaaac atctagtgaa
    10321 tttggccaag attctgctat tgactgacgt acaggatcca catcataatc catttcaaaa
    10381 tctggtatga ttttttgaat agatgctttg atctcttccg ggtcaaaaga catcgccgat
    10441 atattgaagg catttcgatg aaccaatcga tcaggatctg cattcatgag ttttataatg
    10501 gcatcgatcg catctggcat atacatcata tccattgatg taccttgatc aatgaaacta
    10561 gtgtactttc cttctttgat tgctgaataa taaatatcga ctgcgtagtc tgtcgtaccg
    10621 cctcctggca aggttgcata agaaatcaat ccagggaaac ggacaccacg agtatctacg
    10681 ccatatttca cataataata atcacatagt agttctccag ctaccttcgt taccccatac
    10741 atggttgtag gacgctgaag agtatcttgt ggggtatgat cttttggagt atttggacca
    10801 aacgcgccga ttgaacttgg cgtaaaaaat ttcagctgga attctctagc gacttccaat
    10861 gcattgacca gcccagtcac gttcaaattc caggctaatt gtggttttgc ttctgcaaca
    10921 gctgacaaaa gagcagccaa atgaatcaat gtgtccactt gatattgttc tgctatgctg
    10981 cgcatttttt cgtaatccat tacatctagg atctcaaaga tatcgttttt ctcaatgact
    11041 tcaggcatgc gtatatctgt cgcaatgaca ttttcactgc ctagttcttg acgtagccgt
    11101 gtgatcaact ctgaaccaat ttgtcctaaa catcctgtca ccaatacttt tttcatgatg
    11161 atcctccctg atctttttta ctactatctt cagatgatta acaatatatg cgtgaacatg
    11221 ttctctaatt ttaaatagac gaaaaaacta aatttagtta aataatttcc atttcttttc
    11281 ctattttttg ataaactgct actgcttcat caagcatttc cctcgtatgt tccgcagtcg
    11341 gcatatttct gatcctgcct gtcccaagag gcacagttgg ataaacgatt ggtttagcat
    11401 aaatcccatg ttcgatcagc ttcttagaga aagtctgagt cactttttcg tctcccaaaa
    11461 tgactggagt aataggtgtt tcagacatac cgatgttata gcctactttt ttcaattctt
    11521 ctttaaaata attagcattt tcccaaactt tttcgacaag ttccggatgt tcttgcatca
    11581 aagtaatcga agccaatgca gctgcagctg cacctggagt tagcgaagta gaaaatagga
    11641 atggccgact tctagctttc aaccagtcga ttagtgtttt cgatccagct acataacccc
    11701 caacaacacc tatagctttc gataacgtgc ccatttgcat atcgattttg tcgcttaatc
    11761 caaaatgctt gacggttcct gcaccttttc cagttacgcc tgagccgtgc gcatcatcga
    11821 cgtaggtgat cagtccatat ttttcaacaa tcggaataat ttccggcaat cgtgcaatgt
    11881 ctccatccat ggagaaaaca ccatctgtga tatacatgat ttttttatat ttcttacttt
    11941 ctatcgcttc ctttgctttt ttctcaaggt ctttcatatc ttgatgttta attcggatga
    12001 tttttgctcc ggataagcgg caaccgtcaa taatagatgc atggtttaat tcatccgata
    12061 agatagcatc ttcttttgtc attactgcag agatagcgcc catattgcag ttgaatccag
    12121 attgaaaggc gattgctgct tctgtccctt tgaattctgc gattttttct tctaattgct
    12181 gatggatttt cagcgaacca ttgatcgttc ggactgctcc tgctcctaca ccataagttt
    12241 cggttgcttc aatacatgct tttttcaatt cttcgcgatt ggcaaatcca agataattgt
    12301 tggatgctag attgatcatc ttttttccgt ctacgatgat acaagcacca ttttcacttt
    12361 ctagtatgtc gatcgtattg tataacccct tttctttcag ttgatccaat tctttctcta
    12421 aatacattgt tagttctttg gaagacattg tctccacctc catattcatc tgtcttgcag
    12481 aacataccaa acatatatcg tgtcattcat ctacaaagca agtatgctat atttttgata
    12541 acgcttacac tgattatgag aattatttaa ccacattttt tatttacagt atctttttac
    12601 tttcaaatga cttatatagt atgaacaatt accttttaga taatgatcta ttttactcta
    12661 cctgggcaga taaatacaac acaaccattg atagaagccc tttcgttaaa atcaattgta
    12721 ttctgaatat ttaattt
//
LOCUS       DACCXO010000080        12719 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.081, whole genome shotgun sequence.
ACCESSION   DACCXO010000080 DACCXO010000000
VERSION     DACCXO010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.081"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            158..730
                     /locus_tag="IVY70_002015"
     CDS             158..730
                     /locus_tag="IVY70_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP7959138.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            962..1579
                     /locus_tag="IVY70_002016"
     CDS             962..1579
                     /locus_tag="IVY70_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006845390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP7959139.1"
                     /translation="MNYIAEKAHTKQYQKGEFLFRSQEKEDTLYIINRGKIRIYRLAD
                     SGKEQLVRILNPGDFTGEWTLFNPDAVHEEYAEATRDTVVCMIQQKDVQDFLEQYPAI
                     SLKLLAEMSKRLESSERQTTQVAVESVITRLVLFLAENVEPEMGNSPTITLPMAKKDI
                     ASYLGTTPETISRKFGELEDEGLIQQLSGKRIKIQDLDDLLLYSE"
     gene            1807..2145
                     /locus_tag="IVY70_002017"
     CDS             1807..2145
                     /locus_tag="IVY70_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003102891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959140.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            2130..2984
                     /locus_tag="IVY70_002018"
     CDS             2130..2984
                     /locus_tag="IVY70_002018"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP7959141.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3131..3364
                     /locus_tag="IVY70_002019"
     CDS             3131..3364
                     /locus_tag="IVY70_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HAP7959142.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            3562..5466
                     /locus_tag="IVY70_002020"
     CDS             3562..5466
                     /locus_tag="IVY70_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP7959143.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISIGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIS"
     gene            5776..6138
                     /locus_tag="IVY70_002021"
     CDS             5776..6138
                     /locus_tag="IVY70_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7959144.1"
                     /translation="MEKVCQVTIVHKEKVNVIKKKLDQKDFSNLLVLGKCFSDSSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVFLKLSENLSMKS"
     gene            6155..6766
                     /locus_tag="IVY70_002022"
     CDS             6155..6766
                     /locus_tag="IVY70_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HAP7959145.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGIRHIFWGQY
                     LGTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVAFVGEEEEEEEEVVEKLE
                     SRGTNRLFWTVALITIASGGDNLGIYIPYFTSLAFSEIIIALIVFAISVAVLCYISYK
                     LAKISFVSETLEKYERIIVPIMFIALGIFIMIENGTIQTLLGF"
     gene            complement(6868..7776)
                     /locus_tag="IVY70_002023"
     CDS             complement(6868..7776)
                     /locus_tag="IVY70_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP7959146.1"
                     /translation="MSKSDKDHNHSNQNQPKKNIKIAFFINLIFSISEFFFGFLFNSV
                     SIMSDAVHDLGDSISIGFAWFFQGYSEKKQDERFTFGYNRFSLLGALITGVVLISGSF
                     FMIYRSVPRLIDPQPVNYSGMFWLSLVAIALNGYAAWILSKGTSKNESMLNLHMLEDV
                     LGWIGVLIVSILMNFTEAYRLDPILSILIALYILYKTVPEFFTTMKILLNGSPENVNV
                     KSLADSIKNVPAVKDLSHFHIWSLDGEENALIITVLIDPSDIDKAREIKEEIAEVAHK
                     SSVENHITIEITTSKEELEKGYSHNT"
     gene            complement(7818..8132)
                     /locus_tag="IVY70_002024"
     CDS             complement(7818..8132)
                     /locus_tag="IVY70_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP7959147.1"
                     /translation="MDIKTTSPIDKGSILSISKLFKVISDPTRLSVLFLLQKKECSVG
                     NIAISLDMEQSAISHQLKTLKDARLVKSRREGKSMLYSLDDLHVFSILEQVLTHVNEL
                     EK"
     gene            8431..10362
                     /locus_tag="IVY70_002025"
     CDS             8431..10362
                     /locus_tag="IVY70_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP7959148.1"
                     /translation="METIQEQQSQEKHNHNHNHDHDHDHGKMPVVLYFVGLALAVIAL
                     FLNEDYQLVQNVLFSIATISAGYHVILLEGLGETIENSKAQKKFIPNSHILMGLAAIG
                     ASLMGNFWEGTLLILIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLILPDGSTKNV
                     DVSELKVGDQLQVLNGDQVPIDGVILSGSTSIDESSITGESIPKEKSKGDGVFGSTIN
                     GTGTFTMKVTKENKDTVFSKILQLVNQNQDNQTKAASIIQKFEPKYVTVVLIAIPLFM
                     LLAPFLLDWTWSQSVYRGLVLLVAASPCALAAATVSATLSTTSNLAKKGVLSKGSSYL
                     SQLADIKAIAFDKTGTLTKGKPEVTNYYFADSVNEENMIDIVVALEKESNHPLADAIL
                     RKFEQKNKLTIEVENQIGKGLTGDYKGRNYRIGKPTSFDDVSDEYSRLNNEWASEGKT
                     VVYVAVDEEVIGLIALMDVPSEYAKETIEYFRKQGIHTTLITGDSEMTGKAVAKQLGI
                     DEVIANVMPEDKSRIIDEQKEKYGVTAMVGDGVNDAPALVNADVGIAMGDGTDVAVEV
                     SDLVLMQNNLSKLVQSHNISSKMNRVIWQNIIFSMAVVAFLIVVSFLGLTDIAISVII
                     HEGSTLVVILNGLRLLKAV"
     gene            10655..11206
                     /locus_tag="IVY70_002026"
     CDS             10655..11206
                     /locus_tag="IVY70_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7959149.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKI
                     TKRYIGINEDEISETLLNFRLGF"
     gene            11519..12061
                     /locus_tag="IVY70_002027"
     CDS             11519..12061
                     /locus_tag="IVY70_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959150.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
     gene            12572..>12719
                     /locus_tag="IVY70_002028"
     CDS             12572..>12719
                     /locus_tag="IVY70_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7959151.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSK"
BASE COUNT         4139 a         2128 c         2653 g         3799 t
ORIGIN      
        1 aatttaatag agaagcctgg cgtctattaa gaaataattt tcttgatgag cgtcaatttt
       61 tttaacggaa atctatccta tactaaattc agtaaagaaa aaggaggaat caaagaaagc
      121 tattttaaac aaaccaaaat tataggagga ataaatcatg actgaaaata aagctactca
      181 agaaagaaca ccccaagaaa gattgcaaga agaacaagaa cacaaggaac atgttcatca
      241 tacgaaaatt aatgcagcgg ccattgcgga tcatctttta ggcaacatgc atacattaca
      301 tgtgaaactg caccaatatc actggtatgt aaaaggagcc aatttcttta cgttgcatga
      361 aaaatttgaa gagttgtata acgaaaatga aaaatggttc gacaaacttg cggagcgtct
      421 aattacttct gggcataaac ctgcttcgac aacggttgaa tttgaaaaat attcgatgct
      481 ttcggaagat gcagttaata aatacgctaa agcagaagac atggttgaaa atattatcga
      541 ggacttcaga agcactcgtg acctaactat tcgcgcgatt cgtttagctc aagacgaggg
      601 tgacgatgct ttagaagata cattgattgc ttttaaaaat gacttagaca agaacatttg
      661 gatgctgcaa gcgttcattg gtaaagaagc attagaagat gatgacgctc ttgatgagga
      721 tgaagattaa ggtaaaagta gaagctgatt tagggattgg aattctttcc agtccttttt
      781 ccattttctt gatgtgcatc aatttttttg tagtaagaga cttatataat aaaagcagcg
      841 aatataaaca aaataaataa agggggagaa tgaattgact aaacataccc atcatgaaat
      901 tggcgaccac gcagcctgta tccgcttggt tccaatattc aatcacttag aggatagcgc
      961 gatgaattac attgctgaaa aagcccacac gaaacagtat caaaaaggag agtttctgtt
     1021 tcgttcacaa gaaaaagagg atactttata tattatcaat cgtgggaaaa tccgtattta
     1081 tcgattggca gactctggca aagagcagct agtgcggatc ttgaaccctg gtgatttcac
     1141 cggagaatgg accttgttca acccggatgc agtacacgag gaatatgccg aagcaacccg
     1201 agataccgtt gtctgtatga ttcagcaaaa agatgtccaa gattttctgg aacaataccc
     1261 agctatttct ttaaaactat tggcggaaat gtctaaacgg ttagaaagtt cagaacgtca
     1321 aacgacacaa gtagccgtgg agagtgtcat tacccgttta gttttgtttt tggcagaaaa
     1381 tgtggaacct gaaatgggca actctcccac catcaccctg ccgatggcaa aaaaggacat
     1441 tgcttcctat ttaggaacga cacctgagac catcagtcgc aaatttggag aattggagga
     1501 cgaaggattg attcaacagc tgtctgggaa aaggatcaag attcaggatt tagatgattt
     1561 attgctttac agcgaataat cggacacact tttttctaca cgttggtgtt ctgtcgctct
     1621 tcattaacaa cgggacagaa ctcagtgctt ttgcttccat ctgtccgttt ttggcttttt
     1681 taagacagaa cgaccttcat tcattggaaa aataaaagca caaacatgat aaccctcaag
     1741 tttttttgtt ttatttttct ttaagatagg gttgtcgaac aaaacgtaaa aataagtagg
     1801 gtgaatgtgc attgtttttg ggtgatagtt gccatcacgt tgtcagcagc gggggcattc
     1861 tttgtcgtcc ctttcaccaa gaacacgatt ggcggcgaag caaaaacgtc taacgttgtt
     1921 gaggcagata ggttagctat ggaaaaagaa gaggaagttg taaaaggagc cggcgtaatc
     1981 ggacacggaa aagtggcgta ttacgaagcg agtgaccagg gagataaaag tgtattcact
     2041 gtttggtttc catggagctt agccggaatc ttgttcagca tcaccatgac tagaatgacg
     2101 cgaaaaattg aagaaatgga gcaaatagaa tgggattcgt attaggcgtt ttgaatcccc
     2161 atgtggtgga gattggttct gttgtgattc attggtatgg agtcattatt gcagcgggca
     2221 tattagcggc tctccagttg agcaccaagg aagcaaagaa aaaaggactg gaagaggata
     2281 ccattatcga tatggcctta tgggcaattc cgattggact cctcggcgct cggctttatt
     2341 atgtgttatt tgaacttggt tattatctgc aaaatccagg acaaatcctt gcgatttgga
     2401 atggcggcat tgcgatttat gggggattga ttgcaggagg gggtacgcta tattggtatg
     2461 caaagaaaaa aggtacttct ttggcactcg ttcttgatat tttagcgccc aatgtattgt
     2521 tagcccagtc cattggtcgt tggggcaatt ttatgaatca ggaagctcat ggtgggccgg
     2581 ttactcgtca attcttggaa aatctttatt tacctgaatt tatcattgaa caaatgaata
     2641 tgaatggtac ctactaccat ccgacttttt tatacgagtc attgtggagt ttgttgggct
     2701 ttgtcctcat cgtcactata cggaaccgaa aacagctttt gcgtcaagga gaagtcgctt
     2761 tgagttacgt tttgtggtac tcagtgggtc ggttcttcat tgaaggtatg cgaacggaca
     2821 gtttatggat tggcgagtgg atccgcgttt cgcaagcctt gtctctggcc ctgtttattg
     2881 gcgcgattgt agtatggttt atacgcagac aagattatcc accaatttct tattattctg
     2941 atggcaataa ggggcagaaa aagactatta agatggtgaa ttgagcaaaa agataaaaag
     3001 aagtgcaaaa cttgatatgc gtcaattttc gtttgctaaa agtgagctat actacagttg
     3061 taaagaggaa aaggcagtgt tcattaatct gctgccgata aaataaaaac acataaagga
     3121 gataggaatt atggaaaaag ctatattgca attagaaacg ttgtcttgtc caagttgtat
     3181 gcaaaaaatt gaaggtgccg tgaaatcagt taacggtatt gataaagaca gcattaaagt
     3241 attattcaac tccagcaaag tcaaaaccaa ttttgattca gctgtcactt cgattgaaga
     3301 gattcaaaaa gctatcgaaa acgtaggcta tccggttctt aaagcaaaag tcaaggccgt
     3361 ttaacagatt aaatgaacta ggcaattctg gaagaaaagc tatttttcca ggattgcttt
     3421 ttttgttgca atccgattcg aggtgcttag aaacgataaa agagaacttt ctaaacttga
     3481 tgtcagtcaa ttaattactt aggcaattat cttatactgt gtttatcaat aaaagagaaa
     3541 ttaaatttgg agggaaaaag catgcaacaa tatatattaa gcaagaaaaa tgttatcacc
     3601 gtcatcagtg gattgttaat cgtactcggt ttcttcagtc actttgtttt agaaaacgta
     3661 ggactttcag agtggtcttt gatcatcgcc tctgtctttg ggattacgcc aattgccatt
     3721 caagcgtttc aagcaatgaa agtcaaagtc atcagtattg atgtcttagt cagtattgca
     3781 gcgattggtg cgctttttat tcaaaattat gaagaatcgg ctattgtcac gttcttattc
     3841 ttattcggac actacttgga acaacgaaca ttgaaccaaa cgcgatctgc tatcaaagaa
     3901 ttgactgaaa tggcacccga aagcgccttg aaacaaatgg ataatggcga atttgaagaa
     3961 gtagaagtgg atgatataga tgaaggggat atcttgctcg ttaaaacggg tgcgaaagtt
     4021 ccagtagatg gcatagtcct tacgggtgaa gggcatatca atgaagctag cattacaggt
     4081 gaagctattc cggtcaacaa gctagctgat gcagaagttt ttgcaggaac cattttagaa
     4141 aacggaacga ttcaaatcag agctgaccga gttggtgagg acaccacatt tggaaaaatt
     4201 attgaactcg tggaagaagc acaagattct aaatccgaag cggaacggtt cattgatcgc
     4261 ttttctaaat actacacacc agctgtcttg gttctggcaa ttgttgtatg ggcgttcagt
     4321 caaaatattg agttagcaat caccatcttg gttctaggtt gcccaggcgc attagttatc
     4381 ggagtgcctg tctcaaacgt tgccggtatt gggaatggtg ctcgtaacgg tgttctttta
     4441 aaagggagtg aagtcattca agacttcagt aatgtggata caattgtatt tgataagaca
     4501 ggtactttaa ctgtcggaaa cccaaccgtt gcagcgactg aactgtatga aaaagatcct
     4561 gctgaaacgc ttggctatct ggccagtgtg gaacgggaat cagatcatcc attagcaaaa
     4621 gcggtcttaa atcaaattgg agaaacaaac ttttatcctg ttgaaggaac tgaagttgtg
     4681 aaaggcggcg gaatcgtttc aagtgtagct ggacatagag tggctgttgg gaatgtggcc
     4741 ttgatggaaa aagaaaatgt cactctcagc aaaaaagtgc aaaaagatgt caaacggttt
     4801 gaacaacaag ggaactctct cgttttgaca gcggtcgacg gtgaattaaa agtactgatg
     4861 ggcattcgcg atcaagtccg tccgggtgtg aaagctaatt tgcaggaatt aaaagacttg
     4921 ggcgtgaaaa atctagtcgt tctttcagga gataaccaag gaaccgttga tgtggtcgcc
     4981 ggcgaacttg gtttgaccga agctcacggc tacatgttgc cggaagacaa atcggcttat
     5041 atcggaaaac ttcaacaacg tggtcaaatt gtagcctttg ttggagatgg cgttaacgat
     5101 agtccgtcct tagctttagc agacattgga atcgcaatgg gaagcggaac ggacgtagcg
     5161 attgaaacat ccgatgtcgt tttaatgaac tcgaacttca gcaacttgcc tcacgcatta
     5221 ggattagtaa aagccaccgc aaataatatg aaacaaaata tcgtgatttc aattggagta
     5281 gtgttggtct tgttgaccag cgtcttcttc agcgaatgga tgaatatgtc tatcggaatg
     5341 ttggttcatg aaggtagtat cttggtggta attttcaatg gcatgagatt aatgaagtat
     5401 aagttgaaag gtcgaaaaga acaaaaagaa gtatcagcac ctgcagagaa agtaaaaata
     5461 tcttaagaat aaattagaag aggctgagac aatagtccat ccttgaaatg acaaaagcgg
     5521 aaactcagat ttttaaatct gggattttcc gcttttttga gttcttgaaa aaaaataaga
     5581 gtatagagta tgttctttat aaaattaatt ttcgtgtaaa tgattaaaaa atttaataat
     5641 aaaatttaaa ttcctattaa gttgtaacac atgaaattgc ttataatagt gtattgaaat
     5701 taaggtagat tgacaaacga gtgatcaaat agatatacta ccttcaaacg accgtttgaa
     5761 tgtgaggtga gatgaatgga aaaagtttgc caagtgacta tcgtacataa agaaaaagta
     5821 aatgtaataa aaaaaaaatt ggatcaaaaa gatttttcaa acttacttgt gttagggaaa
     5881 tgtttcagcg attcttctag gattaaaatt ttctatgctt tagaaaccta taaggaaatg
     5941 tgtgtctgtg atttagcaga aatacttact gctagtgtcg ctacaacgtc acaccattta
     6001 cgttttttaa aaaaacatgg aatggcaaaa tcacgtcaag atggaaaagt agtttattat
     6061 tctttagcga atgaagacat cctttccgct attcgtgttt ttttaaagtt atcagaaaat
     6121 ctatctatga aatcttagtt tggaggaagt tattttgtta caaagtattt tttcagctat
     6181 cgctgtttat atttctacca gtattgatta tttgtttatc ttgctgatta tcttttctca
     6241 aagccacaca aaaaaaggta ttcgtcacat tttttggggt caatatcttg gaacaggtat
     6301 tttggtagcc gtaagtttgt ttgctgctta tgtgctaaat tttattccgc aagattggat
     6361 cattggtctt ctcggtctaa ttccgatttt cttaggaatc agagtcgctt ttgttggcga
     6421 agaggaggaa gaagaagagg aagtcgttga aaaactcgaa tctagaggaa ctaatcgctt
     6481 attctggaca gttgccttaa taacgattgc ttctggaggc gataatttag gtatttatat
     6541 tccatacttt acctcgttag ctttttcaga aatcattatt gctttaatcg tattcgctat
     6601 ctctgttgcg gttctctgct atatcagtta caaattagcc aagatttctt ttgtttccga
     6661 aacgttagaa aaatacgaac gaatcattgt ccctataatg tttattgcgt taggaatttt
     6721 tattatgata gagaacggga cgatacaaac gctactaggt ttttaaaaat cataatcacg
     6781 aaagataaaa agagaggcag cgactaatca aaatcgctgc cttttttgct tttcaaataa
     6841 tgaagtattt tttagcgtag aactatctta cgtgttgtgg gagtatccct tttcaagctc
     6901 ttctttgctt gtagttattt ctattgtgat atgattctct acgcttgatt tgtgtgcgac
     6961 ttctgcaatt tcttctttta tttccctagc cttgtctatg tctgaagggt ctataagaac
     7021 cgtgatgata agggcgtttt cttccccgtc cagtgaccaa atgtgaaaat gtgagaggtc
     7081 ttttacagca ggaacatttt taatggagtc cgccaagctc tttacgttta cattttcagg
     7141 cgaaccattc aataaaattt tcattgtggt gaaaaattca ggaacggttt tgtagaggat
     7201 ataaagtgca atcaaaatcg ataagatagg atccagacga tatgcctcgg taaagttcat
     7261 tagaatactc acaatcaaga ctccaatcca ccctaatacg tcttccagca tatggaggtt
     7321 caacatgctc tcgtttttgg atgtgccttt gcttaaaatc cacgcagcat atccattcaa
     7381 tgcaatagct acgagagaaa gccagaacat gccactataa tttacgggtt gtgggtcaat
     7441 caaacgtggc acactacgat agatcataaa aaacgagcca ctaattagca caacgccagt
     7501 tatcagcgcg cccagtaaag aaaagcggtt atagccaaaa gtgaaccgtt catcttgttt
     7561 tttctctgaa taaccttgaa aaaaccaagc gaatccaatt gaaatagagt cccctaaatc
     7621 atgaactgcg tcggacataa tggacacact attgaaaaga aagccaaaga aaaattcgct
     7681 tatagagaaa ataagattta tgaagaaagc aattttaata ttctttttcg gttgattctg
     7741 attcgagtga ttatggtctt tatctgattt tgacatttaa aaacctccta agtttgtatg
     7801 ctacatacta attggtttta tttttctaat tcattaacat gagttaagac ttgttcgaga
     7861 atactaaaaa catgaaggtc atctaaactg taaagcatac tttttccttc tcttcttgat
     7921 ttcactaagc gtgcgtcttt caacgttttt aattgatgag aaatagcgga ctgttccatg
     7981 tccagagaaa tagcgatatt tccaacacta cactcttttt tctgtaaaag aaagagaacc
     8041 gacaatcgtg taggatcact aatcacctta aatagtttgc ttattgacag aatagatcct
     8101 ttatcgattg gagaagttgt ttttatatcc atagtgtttt tattcctttc atatgacctt
     8161 ccgttcatat ctatattacc atatgagcga aacaatgcta atcttttgtt tataaaagag
     8221 aagtggcgtt ctctttatct tgacaaaatg tatatagcga tataaaatga gtatacaaac
     8281 atatgattaa ttagtcatat gtaaggtgga ttcattacga tttacttttg gaataaacga
     8341 aaggagtcag aaatagcaga aatttatgtg attcaagaag taaatgatgg aaaacaaagg
     8401 aattataaat tatataggga ggcaatagaa gtggaaacta tccaagaaca acagtctcaa
     8461 gaaaagcata atcataatca caaccacgat cacgaccatg atcatgggaa aatgccagtc
     8521 gttttgtact ttgttggttt agcattagca gtgattgctc tatttttgaa tgaagattat
     8581 cagttggtac aaaatgtttt gttctcaatc gctacaatca gtgccgggta tcacgttatt
     8641 cttcttgaag gccttggaga aacaattgag aattcaaaag cacaaaaaaa gttcatacct
     8701 aattctcata ttttaatggg attagcggca attggagctt ctttgatggg gaatttttgg
     8761 gaaggaacat tattgattct tattttttct ggcgcacatt ttttggaaga ttatgcagaa
     8821 ggaagaagca aacgagaaat aacgaaatta ctcgaaatga atccaacgac tgcccggtta
     8881 atcctgcctg atggtagtac aaaaaatgtg gatgtcagtg aattaaaagt gggagatcaa
     8941 cttcaagtgt tgaatggcga tcaagtacct attgatggcg tcattttgtc tggatctaca
     9001 tccattgatg agtcgtctat tactggagaa agtatcccga aagagaaatc caaaggagat
     9061 ggcgtcttcg ggagtacaat caatggaaca ggtaccttca cgatgaaagt caccaaagaa
     9121 aacaaagata cagtattttc aaaaatttta caattagtga atcagaacca agataaccaa
     9181 acaaaagctg cgagtattat ccaaaaattt gaaccgaaat atgtcacagt tgttttaatt
     9241 gccattcctc tattcatgtt attggctcct tttctacttg attggacatg gtcacaaagt
     9301 gtctatagag gattggtcct tttagttgca gcttcaccgt gtgctttagc agcagctact
     9361 gtctcggcaa cgctatcgac gacttctaat ttagccaaaa aaggagtcct ttcaaaaggt
     9421 agttcttacc tgtctcaatt agccgatatt aaagcaattg catttgataa gacgggcacc
     9481 ttgaccaaag gaaaacctga agtaaccaat tattattttg ctgattccgt gaacgaagaa
     9541 aacatgattg atatcgtagt agctcttgaa aaagaatcca atcacccctt agcagatgct
     9601 attctaagaa agtttgagca aaaaaacaaa ctaactattg aagtagaaaa ccaaattggt
     9661 aaaggattga cgggagatta caaagggaga aattatcgta taggaaaacc gacttcgttt
     9721 gatgatgttt cagatgaata tagtcgttta aataatgaat gggcgtcaga aggtaagaca
     9781 gtcgtatacg tagcagtaga tgaagaggtt ataggcctta ttgcccttat ggacgttccg
     9841 agtgaatatg ctaaagaaac cattgaatat tttaggaaac aaggtataca caccacatta
     9901 attactggtg actcagaaat gacgggaaaa gcggttgcta aacaattagg aatagatgaa
     9961 gtgattgcga atgtcatgcc ggaagacaaa tccagaatta tagacgaaca aaaagaaaaa
    10021 tacggcgtga ccgccatggt tggagacggt gtaaatgatg ctcctgctct tgttaatgca
    10081 gatgtgggaa tcgctatggg agatgggact gatgtggcag tagaggtatc tgatttagtt
    10141 ttaatgcaaa acaatttatc taaattggta caatctcata atatttcttc gaaaatgaac
    10201 cgtgttattt ggcagaatat tattttttca atggccgttg ttgctttttt aattgtcgtt
    10261 agtttcttag gcttaacgga tattgcaatc agtgtcatta ttcatgaagg aagtactttg
    10321 gttgttattt tgaatggact tcgattgcta aaggcagtct aaagaatgat tgcacaactt
    10381 caaattaatc gagtaataga gataattaag gattacacta cattttttat ctctattact
    10441 tgtttatatt tatctagatg ttcaaagtca aaatgatata tcaaccatac tcttgtggcg
    10501 cgggttgaat ataagataca tgaaattgga taaaatcatg tatcaagaat cctatcctta
    10561 tagggttctt tttttttact ccccctaaac acacgatttc gtgttgtaat aactataatc
    10621 atgtattcga acagactaaa aaggagggat tgtcatgggc tataatgttc agccactacg
    10681 gtcgcaacag gagattaatg attttttatt ttgcctgcga cggaataaaa atgcggaacg
    10741 tgacgttttc ttgtttctaa ttggaattaa cagcggtctg cgcatgtccg acatcgttaa
    10801 actcaagaaa aaagacgtga ttacctcaaa aaacccgcgc atcgtggagc aaaagacagg
    10861 gaaaacacgc attttatact taagcagctt acaggacttg attcacggct ataccgcacc
    10921 cttagatccg gaggcttact tgtttcccag tactaagggc ggccatttag aagtcaatac
    10981 ggtctaccag atgtttcaaa aggtcgcgaa gctgttagga agggacgata tcggcaccca
    11041 caccctacga aaaacctttg gttaccacta ttacaagaaa accaaagacg tggccacctt
    11101 aatggaaatc tttggtcaca gcagcgagaa gattaccaaa cgctacatcg gaattaatga
    11161 agatgaaatc agtgaaacgt tattaaattt tcggttaggt ttttgattgt attatataat
    11221 agataagcac agtaagacgg ttataatcac ccacagtatt ggacaaatca accttttttt
    11281 ggtatattat atatacaaat gagagcaggc tcatagtcaa cttgatataa atccggaaca
    11341 taagggtccg gcgggtggcg agcaccaaaa ctaaactcga tacaaatccg gaacgtaagg
    11401 gtccggcggg tggcaagcac caaaactaaa cctgtacaaa aggctgctct tttgagcagc
    11461 tttttttatt gggaaaaagg agcattcttc gcacgcggca aaaaaagaat acaaacgcat
    11521 gtctataaaa gagacaaccc aaattaccag acagttaaac gcgatttata aggctgctca
    11581 tctcttacaa gaacattttg tagataaaaa agtatccttt gtcggagaag tctcaacggt
    11641 agcgattatt ttttcgacaa ctaattttat gcacctctgt ggcattgatt atcgcagagg
    11701 cacacacttg ttttttcaag atgccttaga caggaaaata aatttacagg atattcaaat
    11761 caaaacggat gggacgacct ttcagaaatt acaagtaata ggtagccttg atttattatt
    11821 ggggaagcat atttctattg ttggcagagg tgtttactct tctcttcgct atgatgcagc
    11881 tattcgaaca aggaaaaaaa tattggctct gtctttgaaa caaaacggat taatctatat
    11941 ccctatctct ttgttaaacc tatcatcaaa ggaaattgga cctggacaaa aagttactgg
    12001 catttttagc gaggatttaa catccgggga acttaaaatg attatggaag tcatcgatta
    12061 atcattaata gtgatatgcg acgtcatttt gaagtaatta ttctattttg ggttataaaa
    12121 taaagcattt taagacgatt tcaaatcatt catgtgggtt atttatcacg gatgagcttg
    12181 aaatcgtctt ttatacgtat caaattgaaa tgttcggcat acaacctatc gttgttacta
    12241 cattactaat tgtcacttgt taaggaactt ggatcctttc taaacattcc ctagagataa
    12301 attttacttt atccctccgt taatatccaa tttaagcacc tttccgattc agggttaatt
    12361 tgcatatcaa gtgcagcttg tgttctagta agattagccc ttttagtccg gctgagttga
    12421 cttatggata taagatatgg tcatagcaaa tagatactac gtatagtctg ttaatttagt
    12481 gtcattaatc ttaagttgga taggctacaa ttaactagac agaaaaatta aggtgtgtag
    12541 actagaagaa aacataccag gaggaatttt tatgtctaag agaacacgaa gaactttttc
    12601 acaagaattc aagcaacaaa tcgtcaatct ttacttagct ggaaagccac gtgtagaaat
    12661 cattcgagaa tatgaactaa cggcttcagc atttgacaaa tgggtaaagc aatctaaaa
//
LOCUS       DACCXO010000081        12510 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.082, whole genome shotgun sequence.
ACCESSION   DACCXO010000081 DACCXO010000000
VERSION     DACCXO010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.082"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            2..74
                     /locus_tag="IVY70_002029"
     tRNA            2..74
                     /locus_tag="IVY70_002029"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="IVY70_002030"
     tRNA            78..150
                     /locus_tag="IVY70_002030"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="IVY70_002031"
     tRNA            155..225
                     /locus_tag="IVY70_002031"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="IVY70_002032"
     tRNA            238..311
                     /locus_tag="IVY70_002032"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="IVY70_002033"
     tRNA            323..394
                     /locus_tag="IVY70_002033"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     gene            660..1853
                     /locus_tag="IVY70_002034"
     CDS             660..1853
                     /locus_tag="IVY70_002034"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP7959152.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="IVY70_002035"
     CDS             1983..3455
                     /locus_tag="IVY70_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7959153.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="IVY70_002036"
     CDS             3538..4326
                     /locus_tag="IVY70_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP7959154.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="IVY70_002037"
     CDS             complement(4393..5061)
                     /locus_tag="IVY70_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP7959155.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="IVY70_002038"
     CDS             5176..6888
                     /locus_tag="IVY70_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7959156.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8660
                     /locus_tag="IVY70_002039"
     CDS             6891..8660
                     /locus_tag="IVY70_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7959157.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(8865..9431)
                     /locus_tag="IVY70_002040"
     CDS             complement(8865..9431)
                     /locus_tag="IVY70_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7959158.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(9431..10387)
                     /locus_tag="IVY70_002041"
     CDS             complement(9431..10387)
                     /locus_tag="IVY70_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP7959159.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(10387..11043)
                     /locus_tag="IVY70_002042"
     CDS             complement(10387..11043)
                     /locus_tag="IVY70_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP7959160.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            11413..>12510
                     /locus_tag="IVY70_002043"
     CDS             11413..>12510
                     /locus_tag="IVY70_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7959161.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFSARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNF"
BASE COUNT         3832 a         2251 c         2672 g         3755 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattatttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaactgt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcacgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaataa agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa
     8701 cagccaatca aaaacagctt ctcatgtttt tgatttggtt ggacttatta ccgcagatat
     8761 aagcttttga gcaccgtgaa taaagaggtt atgaaatcaa cgctctaacc tgagtattag
     8821 agaaaaaaac gcaaaacata tcatttatgt tttgcgtttt ttatttatct ttttttctca
     8881 atacaatata aaataggcgg ctggttcttt tggttgataa accgataatt cagcacatta
     8941 tatgtttcct gtggcagttt ggaacaaaaa gaatcaacca tatccagttc tttttctcca
     9001 ccttcatgac catagtagac gacaaggatc aatcgtccgc gaggcacaag tcgtttcaat
     9061 atttcttcca tagctgtgcg tgtagtctct ggcatagtga tgattgcttt atcacttttt
     9121 gggagatacc ctagattgaa aatccctgct ttgagatttt gttgttcagc tatcacttct
     9181 cctaacgttt cgtggccttg tagaaacaat gagacacgtt cttctaactg acgctgttct
     9241 agcctttcac gagtagcatc gattgcttgt tgctggatat caaaactata gacatgacca
     9301 gtttttccga tgtgttctgc tagaaaaacc gtatcgtggc cattgcccat cgttgcatct
     9361 acgacgtgat cccctggttg caagatttct tgcagtaatg tatggctgaa atgcaaagca
     9421 gtttgcaaca tcagacgctc acctttttcc gtacatcata ttttccttgg aagctatctc
     9481 tgcgaagaag ttcttcatca attgcattca agacttccca ttttttcaag ctccacattg
     9541 gtccaatcaa ggaatcccac ggcgcatccc ctgtcaaacg atggatgatg atctcttttg
     9601 gtatcatttc cagctggtcg caaatgacat tgacatagtc aggacggctc attaattgaa
     9661 ggcgaccttc atgatagtcc cgcaacattc tcgttttgcg catcagatgc atcaaatgga
     9721 gcttgattcc ttggatatct gaatctaaaa tggtgcgtct gacattttct ttcatcattt
     9781 ctaaactttc gcctggtaat ccgttgatca aatgcgtaca aacacggata ttatgtttgc
     9841 gtaatttagc cacgccatct aaataagttt gataatcatg tgctcggttg atcagttttg
     9901 aagtttcttc aaaagttgtt tgcaatccaa gttctaccca taaatacatc cgctcgttca
     9961 gttcagcaag gtaatcaact acttcatctg gcagacaatc cggtcgtgta ccgactgata
    10021 atcctacgac acctggtaaa ttgactactt gctcaaatcg ttctttgatt atttcaactg
    10081 gcgcatgggt gtttgtaaaa ttttggaaat aaacgatgta ttgttggaca tgaggccatt
    10141 tttgatgcat catgtcgatt tctttctgga attggattgg aagagggtct tcaggtgcaa
    10201 cgatcatgtc tcccgaacca gatacactgc aaaaagtaca accgccatgg gcaacagtcc
    10261 catctcgatt cggacagtca aatcctccat cgatcggcac cttgaaaatt ttcccgccaa
    10321 actcatttcg taaagcgtaa ttccaagaat gatatctttt atttccttct gaataaggaa
    10381 aagtcattaa aagtccttct ttctgtaaga gttatctatt tgttttcttt tttctctata
    10441 cactggataa gtcaggcaca gccaagaaag actgagtaag tagccgccga tgatatcagt
    10501 aggaaaatga acgcccagat aaatgcggct tatcccgaca cttactataa taaacatcat
    10561 cagcaattga agcaatgtgc gtaaggttct tttttgaaca aaagctggaa gcagaaatag
    10621 taaagaaccg taaagaatca tactgcctgc tgcatgtcca ctagggaaac tgtaacttgt
    10681 ttcaactact aagtgcgcca atgaaggacg ctctcgcatg aaaaatagtt tgatcaccgg
    10741 gttgatgatt ccagcaatta caataaatcc agaaacgaac caaaatgttt ctgccctttt
    10801 tttcttatac cacatccata aagccgcaca agcaaataaa atgatgacgg taagaggatt
    10861 accgaattta gtcataaaga gttggtagct gttcagatca ggataagaag ctctgacaat
    10921 gcctgtaaac cactgatcga aaccagaaag ccacgacaga tggaacttta cgatccagcc
    10981 caaaatgaaa aacaggacaa gaaagaagct accagccacc tgaaatgatg ttttatattt
    11041 catacaattc ctcctttgaa gcaatgactt attatagcat ttttggtcca gcgaaaaaaa
    11101 cggtaaagag ataaaaccaa tgtatgattc ctctttgaat aaccaatatt cttataaaaa
    11161 aaataactgt aatcggaaag acattttttg gtaatgcaaa tgaaatgtta gtgaaataaa
    11221 atcagtgttt ttcgaaatcc ttttaaacaa gaacgtagac gtaggaagaa cttgaaaatg
    11281 aacagaagtt gttcattacg gtaaattaca atatgattac aaaaaacaac aaagctctta
    11341 aataactttt aataaatatt aagagaagtt ataattatta ataatagata aattgaagtt
    11401 ggaggataga agatggaaaa cattgcaaga aaagaacgga gacgtttaga acagaccaaa
    11461 cgttttcgaa aagcaaaacg aggggcagca atcgtgggaa ctgcgatggt aggctgttct
    11521 gtcgctgccc cattagtaca gccggttcct gttcaagcgg atgaaacacc tgttcagttc
    11581 agcgcaagaa tcaatacagc ggcatttatc acagaaattg ctacttatgc tcaaccaatc
    11641 gctcaagcca acgatctata tgcatcagtg atgatcgcac aagcagttgt agaaagcggt
    11701 tggggcagta gtgcgttatc tcaagcacct tattataatc tttttgggat aaaaggcagc
    11761 tatcaaggac aaaccgtcta catggatacg cttgaatttt taaataatca atgggtaaat
    11821 aaaaaggagc ctttccgaca atatccttct tttgctgaat ctttcaatga caatgcctat
    11881 gtacttagaa atacttcatt tggcaacggt tattactatg ccggtacttg gaagagcaac
    11941 acgaaatcct atacagatgc gacagcttgt ttgacagggc gttatgcgac tgatcctggt
    12001 tacgcaggca aattaaataa tatcatcgca acttacggat tgacccaata tgatacacca
    12061 gctagcggaa acgcaggcgg tggagcgact acaggtggca ataatactgg aagtgcaggt
    12121 agtactggcg gttcaagcaa ttctggtaat acaaactcag gttcagcaac ttcgaccact
    12181 tatactgtcc aatctggtga ttctgtttgg ggaatctcaa ataaattcgg tatcacaatg
    12241 gatcaattga tccaatggaa caacatccaa aacaatttca tttatccagg acaaaaactg
    12301 acaatcaaag gtagccagga aaatggctct tcgacgaata attccggtaa caataccaat
    12361 tcttcaggaa atgctggtac atcaaacgga ggacaaacaa ctggtgcgaa atacactgtt
    12421 caatctggtg attctgtttg gaagatctca aatgatcacg gaattacgat ggctcaattg
    12481 attgaatgga acaatatcaa gaacaatttc
//
LOCUS       DACCXO010000082        12347 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.083, whole genome shotgun sequence.
ACCESSION   DACCXO010000082 DACCXO010000000
VERSION     DACCXO010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.083"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(116..319)
                     /locus_tag="IVY70_002044"
     CDS             complement(116..319)
                     /locus_tag="IVY70_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAP7959162.1"
                     /translation="MNNRHRRVAKLRNQELNVAKVKFEREYGVSAEETYKVASQCVAD
                     ASDAIRKFGISILNDDRKWEAEK"
     gene            complement(343..648)
                     /locus_tag="IVY70_002045"
     CDS             complement(343..648)
                     /locus_tag="IVY70_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959163.1"
                     /translation="MNVQVYLKRDLARFPRSWDTNSHPSEAEEEGYLMSANMIEITRD
                     DADEFDKKRSWYSVPNPMYTAVIEDYHISDRFILIDLERPKKRIKQYRRCGFSTKKW"
     gene            complement(815..1384)
                     /locus_tag="IVY70_002046"
     CDS             complement(815..1384)
                     /locus_tag="IVY70_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP7959164.1"
                     /translation="MNKQEAIKKLESIKVIGNDALAACYNESINAGITLMKKIDEPKK
                     LVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLYSVIIAGEYLVKEISCSNEVKLVSTAYLPQYLAH
                     HYQLTEKQIKAIDERYWPFAVPVEEVAEG"
     gene            complement(1530..1865)
                     /locus_tag="IVY70_002047"
     CDS             complement(1530..1865)
                     /locus_tag="IVY70_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959165.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDIHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEQ"
     gene            complement(1862..2248)
                     /locus_tag="IVY70_002048"
     CDS             complement(1862..2248)
                     /locus_tag="IVY70_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959166.1"
                     /translation="MIPRFRSWYTPFKGKTIGQEMKYGQAGRLITHAEMAPDKYVLMQ
                     STGLKDKNGVEIFEGDVVLVNVSNGFDHLVNEKTVVQESEFHSGLVCKSLISGMEYRI
                     FSHQKMGYEYEVTGNIHENSELLEVE"
     gene            complement(2245..2562)
                     /locus_tag="IVY70_002049"
     CDS             complement(2245..2562)
                     /locus_tag="IVY70_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP7959167.1"
                     /translation="MNLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILDEYME
                     GSK"
     gene            complement(2562..2867)
                     /locus_tag="IVY70_002050"
     CDS             complement(2562..2867)
                     /locus_tag="IVY70_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959168.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKTR
                     RRMPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKNMDIDSKGRQFRYVEEK"
     gene            complement(2864..3025)
                     /locus_tag="IVY70_002051"
     CDS             complement(2864..3025)
                     /locus_tag="IVY70_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP7959169.1"
                     /translation="MTTEEVIQMRIRSIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            complement(3040..3399)
                     /locus_tag="IVY70_002052"
     CDS             complement(3040..3399)
                     /locus_tag="IVY70_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP7959170.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            complement(3396..4247)
                     /locus_tag="IVY70_002053"
     CDS             complement(3396..4247)
                     /locus_tag="IVY70_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7959171.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(4264..5094)
                     /locus_tag="IVY70_002054"
     CDS             complement(4264..5094)
                     /locus_tag="IVY70_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP7959172.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            complement(5097..5783)
                     /locus_tag="IVY70_002055"
     CDS             complement(5097..5783)
                     /locus_tag="IVY70_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959173.1"
                     /translation="MFKPLIDSYSAVLKKFKGNDIGATINEEVNIERLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            complement(5789..6535)
                     /locus_tag="IVY70_002056"
     CDS             complement(5789..6535)
                     /locus_tag="IVY70_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP7959174.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPEDVSMLEPATQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWLEDQYREETKEK"
     gene            complement(6528..6869)
                     /locus_tag="IVY70_002057"
     CDS             complement(6528..6869)
                     /locus_tag="IVY70_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959175.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKTEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            complement(7048..7755)
                     /locus_tag="IVY70_002058"
     CDS             complement(7048..7755)
                     /locus_tag="IVY70_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP7959176.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFDKKHRHVLTAIDELKEGVAEN
                     WADLFWEDTYVHPQNKQSYRIIYMNRDGFSLLAMGFTGKKALSFKLQYIEAFNKMEKE
                     LKDQLPSIPATKREMALLALAANEETNERVDAIESDLNDLKNNQLLAEPDYRTISNMV
                     RNKIRVICNQQHLNSKAKAELFKDLNGGIKRITGAVARNRIKAKQFDDVIDFINNWMP
                     STATMTIIKQMELLEDE"
     gene            7800..8279
                     /locus_tag="IVY70_002059"
     CDS             7800..8279
                     /locus_tag="IVY70_002059"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959177.1"
                     /translation="MSRKIDNIILLTTIFYLVIGATLSLLSFLSNSSLIVLNQKSILT
                     EFAHIDYFSFFKTGLICLFLSSFILYIGTTFCLMVLNLKMFIIEKWIVIIAKIFSSIS
                     IIIPMTLTFSKEQFNVATTFVSFIALFSFAVPKTFQSKSSNAKVNVENTDRKNDESY"
     gene            complement(8484..8735)
                     /locus_tag="IVY70_002060"
     CDS             complement(8484..8735)
                     /locus_tag="IVY70_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7959178.1"
                     /translation="MEQLALVNLSALKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWANSTEH"
     gene            complement(8749..8940)
                     /locus_tag="IVY70_002061"
     CDS             complement(8749..8940)
                     /locus_tag="IVY70_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959179.1"
                     /translation="MSNLDNGRAAIKKFMRENGVTMEDLATAYGYTRVRMQQIIDGHW
                     SGPKANKTILEIIRDYRIR"
     gene            9233..9553
                     /locus_tag="IVY70_002062"
     CDS             9233..9553
                     /locus_tag="IVY70_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7959180.1"
                     /translation="MTLFERIKSLASQRDKSMKEVALELGFSENLFYRWKTTEPKARD
                     LQKVADYFNVSVDYLLGREEKETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            9571..9993
                     /locus_tag="IVY70_002063"
     CDS             9571..9993
                     /locus_tag="IVY70_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP7959181.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFEAEADRIAIMILLNIFIENELTDE
                     SQFKLENFMEFYSINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            10076..10711
                     /locus_tag="IVY70_002064"
     CDS             10076..10711
                     /locus_tag="IVY70_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP7959182.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAQNNVLQAQTGYFGTETVSIALHN"
     gene            10829..12040
                     /locus_tag="IVY70_002065"
     CDS             10829..12040
                     /locus_tag="IVY70_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7959183.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKANSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLRHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFEKLLKKDIK"
BASE COUNT         3576 a         2461 c         1965 g         4345 t
ORIGIN      
        1 accatgtcac cgactttgaa ttttgatttc ttgcgtgttt tagggttctt tgtcggcata
       61 tctagcatta aaccgccgat accgtgactg ctagcgtaaa atccgtcttt tagtttcatt
      121 tttctgcctc ccatttacga tcatcattta atatcgaaat cccaaactta cgaatagcat
      181 cacttgcatc agcaacacac tgacttgcca ctttatatgt ttcttctgca gaaacaccat
      241 actcccgttc gaactttact ttcgctacat tcagttcttg atttcttagt tttgctactc
      301 tgcggtgtct gttgttcatt tttcttcacc tctgttttgt tatcaccatt tctttgttga
      361 aaatccacaa cgtctatact gtttaattcg tttcttaggc ctttctagat caatcagaat
      421 aaatctgtcg gatatatgat aatcttctat tacagccgta tacattggat tcggaaccga
      481 gtaccatgat ctttttttat caaactcatc cgcatcatct cttgtaattt ctatcatgtt
      541 agcagacatt agatagccct cttcttctgc ctcggatggg tgggaatttg tatcccagct
      601 tcgaggaaac ctggccaaat ctctttttaa ataaacttga acattcattc cgcttcctcc
      661 tcatcgatct ctatctcgac atctctgcct tctaaaatct gtttggtatc aatgcttttt
      721 gttttcaacg ccaacccacc gtctttttgt gcagcaacaa tggtaatcgt cattgccttt
      781 gttccgtctg gtatatggag cattatctgt ctactcatcc ttccgccacc tcttccactg
      841 gcacagcaaa tggccagtat ctttcatcaa ttgctttgat ctgtttttcg gtcagttggt
      901 agtggtgtgc aaggtattgt ggcaggtaag ctgttgatac tagtttaact tcattactgc
      961 aactgatctc cttgacaagg tactcgccag caattattac actgtacaac ggctctttct
     1021 cgacctcgta gccgttgtac aagctcaata gtgtttcata tgattgtctc gttgcccaag
     1081 cgagtaattc ttctccttgt tcttgcgtca ctatgccatc tcgtacaaac caatcacaga
     1141 aataatatga atcaccatct ttagatttga ttaaatacgc tattttttca gctttcgtaa
     1201 aagggtccgt agatttctca agccaaccgg ccacgagttg tggaataact agcttcttcg
     1261 gttcgtctat ttttttcatt aacgtaatac ctgcatttat actctcgtta tagcaagcag
     1321 ctagtgcatc atttcctatc actttaatac tttctaactt tttgattgct tcctgtttat
     1381 tcatcgctgt tcctcctaaa aaattaactg ccaaagcaat ataatcactt tcaatgatac
     1441 aaatcccacc agtgcaataa ttacagatat aaacgcacag tttatgaaac agccaactaa
     1501 ggcgcttgct tcttctaacc agtctttcat tactgttcct ccttcaacca taaatttgga
     1561 aaatcatcgg catatatctc tattgcgcca aataacttga tcgctgattt tgattttcca
     1621 tgtatgtcag gactgattaa acgatcataa taatcctgac tgattgtttt tcccttgtac
     1681 tgataacgtg tagccgcagt gtaatatact gctgtcttgc tcagataaag atattttgct
     1741 aaatcagccg atatccgtaa caaaatgatt ctattcttac ttttaattcc acaatttagc
     1801 aatgctggga cattcccatt ttcatctttt aaagaatcga tgtagtcagt agctaagata
     1861 atcactctac ctccaataac tcgctattct cgtgtatatt tccggtgact tcatattcgt
     1921 atcccatctt ttggtggctg aatattctat attccattcc actaattaat gatttacaaa
     1981 ctaatccgga atgaaactcg gattcttgta caaccgtttt ttcattaact aaatgatcaa
     2041 aaccattact tacattaacc agaactacat ccccttcaaa tatttctacg ccattcttat
     2101 ctttcagtcc tgtggattgc ataagcacat atttatctgg agccatttca gcatgagtga
     2161 tcaaccttcc tgcttgccca tatttcattt cttgtccaat tgttttacct ttaaatggtg
     2221 tgtaccacga tcgaaatctc ggtatcattt gctgccctcc atgtattcgt ctaatatctc
     2281 tttatatttc tctacaaatt tgaaacgatc ttgatgaagt ttcttgctcc aatttgtttg
     2341 ccgatccagc tcacgcattt gatcgaaccc tttttgaatt tcgttgtaat aaaattcaat
     2401 gtttgctgct gccttccaat gcctgctact tcgcactcct gcccctgttt cagccatttc
     2461 caacttaact aattccgctc gttcttttga ttttttgtct ttctgaatct tcatcatgat
     2521 tttcttgagg atgatatcac tgtattgtgt aatgagattc attatttctc ctccacatac
     2581 ctaaactgtc ttcctttaga atcaatatcc atattcttcg ctctatccca gatgatgttt
     2641 ttgctcagac cagtaatttc agataactgt tcagcggtac ctgttactag aattcggtca
     2701 ccgtgccaga ttgcaatttt tctcggcatt ctccgtctgg ttttttcagt ccacattgat
     2761 ttaccgagct tttggacttc tgcaactatt tctttgtctt cttgccaatt ctcagaatgt
     2821 gtcagttcga tgattcgttt cattgctgct ttcttatcca cgctcattcc tccaatctac
     2881 gaatttccct tcttaagttc tctatgtgca aatcgattgc ctttctcgcc gtttcattga
     2941 ccatcactgc ctttgttcgt tccagatcgt caatctcacg ttgaatgctt cgaattcgca
     3001 tttgaatcac ttcttctgtt gtcatgatag accacctctt taaaaacgct cttccttgaa
     3061 cgtattccga tattttttag ctaatatcaa cggcacttga tattgatgac aaaacagctt
     3121 tgccttgatc ttaaagtctt ttgtctgcat tcctttaaca tctacgactt tgacaagttt
     3181 gccgttttta taaaatgtga agtcgggaat atactcgatc ttgcgatact tctttccgtc
     3241 tagttcaaat ttcggcatca gctcaaatct ttcctgaagt tttactttcc agccatttgc
     3301 ttcagcttgc cacaaggcta gatcgtaata ctctgcttct gcgatagaat caaacttgat
     3361 acctcgatga acagtttttt tattacggta tttattcatg cgatactacc tttcactggt
     3421 tttatgcgct tgtctgctgt ttgttggaat ttcagcgcat aaccttctga attcttaaat
     3481 atcctagaaa caattctttc accgtaggct tctcttagtt caggaccaga taagtttgtt
     3541 gtgatgatcg ttgccttgtt ctgtctggct tctaagagcg tgtttaacgt gttgtttgta
     3601 aactgcctac tatttgatac cccactacct aattcagctc caatatcgtc aaaaaccacc
     3661 aaatcagttg ttttaatatc ggctataagc gatccttcaa tttcttttct cagttcagta
     3721 ttgttataag aaaactttat ttgctctaat aattcttgat agcttataaa aagtattttc
     3781 ttgtcataat ttgagcgctc aagtatttcc caagctgtgg ccattgataa gtggcttttt
     3841 ccgcttcctg atttccctga tagaatgaaa tgtgcaggat ggttcagcag aacctcattc
     3901 gtatagcttt ttgctttttc taaagcgatt tttgtttctt gatccactac gtgataattt
     3961 tccattttgc atttaaacaa agttttatct gttaataccg aaccattttg aaaaaaactc
     4021 aacgctcgtg cttttaagct atcgttatat atccgttcgg tctgtatatc ctctttcaca
     4081 cgtaacgctt tataaccaca actcatgcat gttggtttac aacgttctga accatcctta
     4141 tttttagctc gccaactata caaaggttcg ctacattctg gacattttcc gctttgcact
     4201 aatactcttc ttattagttt ctccatagca tttgctaggc tttccatgtg atgcatctcc
     4261 tttttaaatt ggcaagtcgt catattcact aggattgctg tactgtagtt tttgactttg
     4321 ctttttatga ttcttcttgt ctgctttgat ttcgaatttg agcttctcaa atttttctct
     4381 caatttctta gcacttctaa tatttccaaa ccaaaattca tttgtaggta gccaattgat
     4441 cacatactca atcgcttcta tagatgcttt atctctttct tccatcaacc tgattgtgtc
     4501 tgcccatttt tcgatatcta ctttgttcat ttcttttgga aaatcttcag ttaaattact
     4561 ttgcaacttt ttagcaaggc gtaagtgttc gtcagaatac ttacctttct tttcttcttt
     4621 atctatatct ttatcttctt ctatatcttt atctgtaccg tcacgtgacg tcacgctaac
     4681 gtcattttcc aatttgagac gttcctgtct ctttctttcc ctgtatttac ggtttctttc
     4741 agcatttttt agccttactt tatccatacc ctcgatattt tgatgttttt cccaattact
     4801 gatggcaatt agtccatcac tgcttagatc aatcatgttg aaatttgcca atgtagttag
     4861 cgctaagcga accgtattta cgtttttgcc aaacaatgta gcaagcattt cttcggtata
     4921 aggcatgttc ctctggatat atatcagacc atcgtcgtta gtctttcctg ctaaaactag
     4981 taatcgaatc catataacga tgatggcatc cgactcagga acagcttgga ttaatcgtat
     5041 tttttcatcg tcaaacatag tagttttaag tttgatccaa cttatctcag ccaaatttat
     5101 cctcctatcc ttaacttttt aattgtttcc tggtttaact tgatcccttt gatttgatat
     5161 ttatttttga aattgatcac acctattttg tgtttctctg tgtgatggat tctgcagagt
     5221 gctgcaaatg tgtactctga atgatcaact tctttgcgct ttcgtcttcc cagcgctttg
     5281 tcaaagtgat cgatatcagc tcctgttttg ccacagatgc agcagactct ttttgtaatg
     5341 catttgtaga agtaatactc ttgattcgct ggtaaaatct catatccttc tttgaaagga
     5401 atatgatgtt caaagatgaa atctaggatg atattcgcta agatattggc atcactcacg
     5461 gttgtattcg attcgtcttt gaggcttatt tcgcgccctg tgacaccttc aaaacggaag
     5521 tagaagaatt ccttccagaa gtccgttggc atgcctgtat cgatgaaaat atcgcctatg
     5581 agcgcataga tgaagtttct ttgctgtact gtgaaccgtc gtggatcaat aaagcgaatt
     5641 tcaatgactc gatcgccatc atatccgtca tacatcgttt tcagccgttc gatatttact
     5701 tcttcgttga ttgttgcacc aatgtcgtta cctttaaatt tctttagtac cgccgagtat
     5761 gaatctatta gtggtttaaa cactcatatc acttctcttt tgtttcttct ctgtactgat
     5821 cttcaagcca attaacgcct cgttttagaa tgcccaagtc tctcttggtc catttactgt
     5881 catcagcggt tatggaagct gcatcagtca atgcaacaat tgcttcatca attgattttt
     5941 cgtacttgtt agcaaccagt tgtaaagcat ccaagaatag ctttttgctt ctttgagtag
     6001 ctggttcaag catcgagaca tcttctggca tatcttcgcc agcaaatata tatagcccta
     6061 gcccaaacat tgctagattt tttacaagac agcgcatgat cgttttgttg atatcaaaca
     6121 tagttgctgc ttcaactcgc ttttcgattt ttccaacaat ctcttttttc ttcgtttcgt
     6181 tattccactg ataatcattg acttcgtagg tatatggctc atctttcatt gccttgtttg
     6241 caccatccat gactggtaac cacatgtcac gctttactcc gttgactgtg atactggtaa
     6301 aaaccatata gcctgttttc ttatcaaaga catacggcag atatgtttct ggatcatgat
     6361 agatttcata atctactgcc tcacacactt tgctaacttc tgcccaagcc catgcccacg
     6421 aaagataagt gaggttattt cgtttttcag taacatcttt cacatttatt ttgtataagg
     6481 tattaaaaag cgtattatca cttctatttt tcaaaggttg ttccttttca ctcatcaaat
     6541 tctgcctcca tttcagcaat gtatttctta cctgatccgt aataagagat atcaatcaag
     6601 ttatctctgt catactcttc tagcgcatca atcaagccat cttcgatgac atagatatat
     6661 tcagtttttt tggacttcct cgataaatgg ataagataga catgatccca aatactcaca
     6721 aaatttccca aatcgtcttg atcacatgct agttcttcat ccgtcaaaag attacgtctg
     6781 atttttcgat tgcttgtttc cttgatattc gatttgcccc aactaggatc agtcaaatat
     6841 tgatctagag tggaaagttc ttttttcatg tggtaacatc tccttagatg tattttcttt
     6901 gtgactcatt gctttggtcg gctgagtcac ttttttattt gttgccatgc tttttgctta
     6961 tcaatatgtt gttggcttag gatgtttggt ttattgtgtc tccaccagcg attagcaatt
     7021 attacgccta tttttagcgc ttcagctcta ttcatcctct aacaactcca tttgcttaat
     7081 aattgtcatc gtcgctgtag aaggcatcca attgttaata aaatcgataa catcatcgaa
     7141 ctgttttgcc ttgatacgat ttctagctac tgctccagtg attcgcttaa ttccgccatt
     7201 caagtcttta aacaattctg cttttgcttt gctatttaaa tgctgctgat tacaaataac
     7261 tctaattttg ttgcgaacca tattagaaat tgttcggtaa tctggttcag ctagaagttg
     7321 attgttcttc aaatcgttta aatcagactc gatagcgtct actctttcat tcgtttcttc
     7381 gtttgcagct agagcaagta atgccatttc tcgtttagtt gctggaattg atggcaattg
     7441 gtcttttaat tctttttcca tcttgttgaa ggcctcaata tattgaagtt taaaacttaa
     7501 agctttctta ccagtgaagc ccattgctaa tagagagaag ccgtctctat tcatataaat
     7561 aattcggtat gattgtttat tttgaggatg aacataagta tcttcccaaa ataggtctgc
     7621 ccaattttcg gcaaccccct cttttagttc atcaatagcc gtcaaaacat gtctgtgctt
     7681 tttatcgaag ctttctgcaa cttgtaagct cgtagtcaca gcttctttat ttttcaaaat
     7741 tactaattct tgcattattt cttctctcct ttttgatata attctggtgg ggtgaaaaca
     7801 tgagcagaaa aattgataat ataatattgt taacaactat tttttattta gtaatcggag
     7861 caactctttc cttactgtca tttttgtcca acagtagttt gattgtgtta aatcaaaaaa
     7921 gcatattaac agaatttgcc cacatagatt acttctcttt ttttaaaact ggactaatat
     7981 gtttatttct atcttcattt atcctctata taggaactac attttgtcta atggtgctta
     8041 atttaaaaat gtttataatt gaaaaatgga tagttattat cgcaaagatt ttctcatcga
     8101 tatcaattat cattccaatg acattgacgt tttctaaaga gcaatttaac gtagctacta
     8161 cattcgtatc ttttatagct ctattttcgt ttgctgttcc taaaactttt caaagcaaat
     8221 ctagcaacgc aaaggttaat gtagaaaaca ctgatagaaa aaatgatgaa agttattaaa
     8281 aaaaataatt ctttcattta ttgtcaaacc tttcttatag acattagact tttttagtca
     8341 gtccctcccg actggctttt tcgctctgta ctcagcttca tcaagcccta taaaaatcca
     8401 aaccatgtac acaatcgttc ctatcaacgc ttgtctattt ccccaaagcc ctaaagcgta
     8461 gatgattagc ggtgcgctga atactagtgc tctgttgaat ttgcccatgt ttttcctcct
     8521 tttagatact gatacaaagc gatactacta aacctccact ctctgccaac tttcgctgct
     8581 ggaatctttc cagattcggc atctttagtt agcgtgcgtg tcgtggtttt caaatattcc
     8641 gcagcttgct ttgtatccca cacttcattt gcaatctcag attctgccaa agaagctttg
     8701 agggctgaga ggttaactaa agctagttgt tccattgatt aattcctcct atcgaattct
     8761 gtaatcacgg atgatttcta agatagtttt attagctttt ggtccactcc aatggccatc
     8821 aataatttgc tgcattcgaa cacgtgtgta tccatatgca gtagccaaat cttccatagt
     8881 tactccgttt tctcgcataa acttcttgat tgcagcacgg ccgttatcta gatttgacat
     8941 ctattcactc cccttacata tagatttgta agtcaaaatg atagaaaaaa cgtataaact
     9001 attgactaat agtatacaat cgtatactat ataaacatag ttaaataagc ctacaacaaa
     9061 ccctttatta tgcaatcggt cgccaaactt aatgctataa ggtgtatttt tagtttgctt
     9121 tttttctatc aaattaactt acaaacaaat aataatacaa acttatactt atgtcaacag
     9181 tataatttac attttgtgta cttttatttg tttaggattg gagaacatta ttatgacact
     9241 gtttgaaagg ataaaatcat tagctagcca aagagataaa agcatgaaag aagtcgcttt
     9301 agaattagga tttagcgaaa atcttttcta tcgatggaaa acaacagaac ccaaagcaag
     9361 agatttgcaa aaggtagctg actatttcaa tgtctctgta gactatcttc taggtagaga
     9421 agaaaaagaa acgcctaaac atgtggattt atcagaagat gatactgtat tttcttttga
     9481 tggaaaagaa atatctaagg agacaatgcg taaagcgatt gcaattgcta aagctttaga
     9541 ggaaaatgaa tagttggagt gatgggttgt atgtatttaa agttgaaaga aatgctgagt
     9601 gagtataatt taaagttaat ctatatggaa atggaagaac caggttttta ttatccaaaa
     9661 ccaagaatag tatttttgaa tgaaaaacta cacgaagaca gttctgaagc ttttcattta
     9721 gcccatgagc tcggtcattt cattgcttca cattttgaat attcagtact gtacgataac
     9781 tctacaactt ttcattcaaa attcgaagct gaagctgata gaatcgcaat tatgatttta
     9841 ctaaatatct ttattgagaa cgaattgact gatgaatctc agttcaaatt agaaaatttc
     9901 atggaattct actctatcaa taataagtta agaacagaat gttttaatgt ttgccagtca
     9961 tatttcaaga aaaaatactc ttatgcacag taaaaaaagc ccgtgctgca acacggactc
    10021 atacctcatt tctgagatca caaatatatt ataacaagaa gtgagggata tttaaatggc
    10081 aaaaaaagtt atgggtcaag atgggaaaat gtataaggtt aaaaaaccgt tttataaacg
    10141 ggtatggttt tgggtattaa tagtactttt gttttttggt attggtggtg ctctaagtgg
    10201 cggtgaaaac aaagattcag ctactgcaac atcaacaagt actaataaag aaactacaaa
    10261 agaaactaca acaaaagaag aaaagactta taaaatcggt gatgacgttg ctgtagggaa
    10321 aatggaatac aaagtaaact ccgttgaagt agtaaaacaa gttggcccat ctgtacttcc
    10381 tactaacgca aaagacactt tcttagtagt tgacttatct gtaaaaaatg caggtgataa
    10441 ggctgtgact gttgatagct cattcttcaa attaaaagca gatggaaaaa ctttcgaagc
    10501 tgattcggct gcatctatgt cggcaaatca agatgaaaat ggtaatatca ctaactcttt
    10561 ctttatggaa agcttaaatc ctgatatgca acaaactggt aaaatagttt ttgatatctc
    10621 tgaagcccaa gcaaatgcac aaaataatgt attgcaagca caaacaggtt actttggaac
    10681 agagacagtt tcaattgctc ttcataacta attagtataa aaaacacgcc ccaccgacca
    10741 aagcgagcgt gttctaagaa aaaacaaacc tacacaatag gcttctttat agtacctatt
    10801 gtatcagaga aagagagctg attcaattat gtcagaagta acaggatact tagaacaagt
    10861 agacaatgaa acatatagac taagagctgt attagggtac aaacctgatg gctcggcaaa
    10921 aagaaaaagc aaaactataa aagcgaatag taaaagagcg gcatataaag aattgaacat
    10981 ctggttagag caattcgaag gaatgaccga tgattcgttg gatttattca atataacttt
    11041 tggtgaattt tatagaaaaa tatggctttc agaggccgaa aaaaatttag agccaaagtc
    11101 ttatcataac tataagcgaa tgatcgagaa tagatttctt gataaatttg attttatccc
    11161 tctaatcgat ataaaaccat acatgataaa gaaaattgtt gttaatgctc aaagaatcaa
    11221 tacaaaagat cctggaagaa actctgataa acctttatca agaaatacaa agcttcgtat
    11281 gttatacgcc gttaacaact tatttttgat ggctaaaaat gagtacggag caattaaaga
    11341 aaatcctgtg gaaaatgtaa aaataccaaa agaaaaaggc gtaaaaaaga atatcgaaga
    11401 accttattcg gaagaagaaa ttcatgcaat gctaaaagca gcatttgagg aaagcataga
    11461 aattaaaact ttaattgttc tagcatttat tacaggagca agacaaggag aaattgcagc
    11521 tttagaagaa aaagatattg actttgacaa acaagaaata agatttcatc agcggatttc
    11581 agaagtggat ggaaaatcag atattcggtt gctgccaggt ttgaaaaatg atgatgatga
    11641 aaaaatagta accggtcctg cttatctatt tgatatgtta gatgaattga tcaaagagaa
    11701 taaaaaaatt cgttggaaat taaatataaa gaaattaaga cactatttta tatttgatac
    11761 aaaacaagac ggaactttac cacgtggaag ttatctgtat aagaagttca aacgattcac
    11821 caaacgtcac aatttgcgtc acattcgttt tcacgacatt agacatacat cagctaccta
    11881 tttgcttagc gatcctaata tgactccaaa agagcttcaa aaacgattag gacaccgaga
    11941 ttttaatact acaatgaatg tttatggcca tgtcttacgc aaagaaaaag atacagcaac
    12001 tacagcgttt gaaaaactat taaaaaaaga tataaaataa cactaaaaaa ttatattttc
    12061 gggtataaac tcgggtagga tatataaaaa cttgggataa tttgcggtat ataagcattt
    12121 aaaaatgctg taaattcgct tatcttcatt taaaatacca ttaaataacg aactcttaat
    12181 cagcgggtcg cgggttcgag cccctcacgg cccattgggt gccaaaccca cggaacaggt
    12241 aaatgttggt gcgaaagcat ttcgaaaaca tttacgctgt tcttttttta ttttcgccgt
    12301 ttgtataatt aagcgagaca aataaaaaag ccatttatgt tacactc
//
LOCUS       DACCXO010000083        12187 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.084, whole genome shotgun sequence.
ACCESSION   DACCXO010000083 DACCXO010000000
VERSION     DACCXO010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.084"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(32..589)
                     /gene="frr"
                     /locus_tag="IVY70_002066"
     CDS             complement(32..589)
                     /gene="frr"
                     /locus_tag="IVY70_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP7959184.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(592..1314)
                     /locus_tag="IVY70_002067"
     CDS             complement(592..1314)
                     /locus_tag="IVY70_002067"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP7959185.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1450..2331)
                     /locus_tag="IVY70_002068"
     CDS             complement(1450..2331)
                     /locus_tag="IVY70_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP7959186.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2430..3212)
                     /gene="rpsB"
                     /locus_tag="IVY70_002069"
     CDS             complement(2430..3212)
                     /gene="rpsB"
                     /locus_tag="IVY70_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP7959187.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3571..4050
                     /locus_tag="IVY70_002070"
     CDS             3571..4050
                     /locus_tag="IVY70_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP7959188.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4267..5358
                     /gene="phnW"
                     /locus_tag="IVY70_002071"
     CDS             4267..5358
                     /gene="phnW"
                     /locus_tag="IVY70_002071"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP7959189.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5351..6136
                     /locus_tag="IVY70_002072"
     CDS             5351..6136
                     /locus_tag="IVY70_002072"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP7959190.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6482..8173)
                     /locus_tag="IVY70_002073"
     CDS             complement(6482..8173)
                     /locus_tag="IVY70_002073"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP7959191.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8595..9542)
                     /gene="arcC"
                     /locus_tag="IVY70_002074"
     CDS             complement(8595..9542)
                     /gene="arcC"
                     /locus_tag="IVY70_002074"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP7959192.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9657..10676)
                     /gene="argF"
                     /locus_tag="IVY70_002075"
     CDS             complement(9657..10676)
                     /gene="argF"
                     /locus_tag="IVY70_002075"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP7959193.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10767..11996)
                     /gene="arcA"
                     /locus_tag="IVY70_002076"
     CDS             complement(10767..11996)
                     /gene="arcA"
                     /locus_tag="IVY70_002076"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP7959194.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3337 a         2538 c         2186 g         4126 t
ORIGIN      
        1 aatgatccgc ccctctgttt ttatgatttt attaaacttc caataattct ttttctttat
       61 cagcagcgat cttatctact tctttaatgc tgttatctgt caatgtttgt acgtcttttt
      121 ctaatccgcg caaatcatct tcggtaatat caccattttt ttgttgtttt ttatattcat
      181 cgatcgcatc acgacggatg ttacgaatcg cgatttttgc attttctgct tcttttttca
      241 catctttagc taattctttt ctgcgttctt ctgtcagttg cgggataact aagcgaatga
      301 catttccatc attggttgga ctgattccga tatcacttgc cataatagct ttttcaacat
      361 cttgaagaat gctcttgtca aaaggtgtga tcattaaaac acgtgcttct ggaatttgga
      421 ttgatgccat ttggttcaat ggtgttggcg caccgtagta gttcacagta atacggtcca
      481 aaaggctagc gttcgcacgg cctgcgcgga tttgcccaag ttcacgttga agattttgtg
      541 ctgctttttt cattttttct tttgcttctg tcataattgc atctgccatt tttatttccc
      601 ccttacagtt gttccgatat tttcgcctaa gattgcacgg cgaatatttc cgtgttcatt
      661 taaattgaag acaagcagtg gaatatcatt atccatgctt agtgaacttg cagttgaatc
      721 catgacttgt aatcctttgg caataacttc cagatgagtc agttcttcaa attttactgc
      781 atttgcatct actttcggat cagcagaata aacaccgtct acattgtttt tagccatcag
      841 gataacatct gcgccgattt ctgccgctcg taatgctgct gttgtgtctg tcgagaagta
      901 cgggttgccg gtaccgcctg caaagataac gatccgtcct ttttctaaat gacgttctgc
      961 tttacggcga atatatggct ctgcgatttg acgcatttca atcgaagttt gcacgcgtgt
     1021 cggtacaccc acgttttcca acgtatcctg taaagctaag gcattcataa ctgtagctaa
     1081 catacccatg taatcagctt gagcacgttc catgcccatt tgtgctccga tttgtccgcg
     1141 ccagatattt ccgccaccca caacgattgc catttcaacg cccagctcgt gaacttcttt
     1201 gatttcttgg atgatttctt tgattactgg tggtttgatt ccaaatcctg catccccagc
     1261 taaagcttct ccactcaatt ttaagactac acgctgatat ttcggtgtga ccatgttcat
     1321 tcctccaata ttttctattg ccattttacc atacttagat gatttcgaca ctctaagact
     1381 ggtttttctt gttttgctta aaaaaaggga gcgcacaaac cgtgcgttcc cgtctgttat
     1441 tcaacagaat tattttttca cttggctcat aacttcttct acaaagttat cttcgcgttt
     1501 ttcgatacct tcgccaactt cgaaacgtac gaatgtttta acagttgcgc cttttgatgc
     1561 aacgtatttt tcaacagtca tatcaggatc tttaacgaat ggttggtcca ccaaagcgat
     1621 ttctgctttg aatttcttca agcgtccttc taccattttt tcaacgatat tcgctggttt
     1681 gccttcgttc aatgcttgtt ctgtaagaac agttttttca tgttctaatt cagcttctgg
     1741 aatttgtgat tcgtttacat aacgaggatt gatagctgct acgtgcatag ctacgtctct
     1801 tgcaactgtt tcgtcagttg ttccatctag aacagtcaat acggcaatac gtccgcccat
     1861 atgcaagtat ccaccaaatg cagcattgtc gtctttttct acaacttcaa agcgacggaa
     1921 gctgattttt tctccgataa cttgagttgc ttcgatcaag tcagattcaa ttgtaccttt
     1981 atctgttttg attttcattg cagcttccat gtctgctggt ttgttttcag caacaagttc
     2041 tgcaatttct tttactaaat cttggaacat ttcgttttta gaaacaaagt ctgtttctga
     2101 gtttacttca acgattgctg cagtgttacc tttcacagca actgaagcca atccttcagc
     2161 tgcaatgcgg tcatttttct tcgcagcctt tgccattcct ttttcacgta aaagatcaac
     2221 tgctttttcc atgtcgcctt ctacttcaac taatgctttt ttcgcatcca tcattcctac
     2281 gccagtcatt tcgcgtagtt cttttaccat tttagctgta acgtctgcca tgtttaactc
     2341 ctcctgtgtg tttttctttt taaaaaaagc tgcttcaaag gagagtgagg cttgtcgccc
     2401 agcctttgaa acagctccat cattcaaaat tattctgctg aagaattgtc gccttcaaca
     2461 acatctacga tttcttcgat tgatgttgct gtgttttctt ctgtgaaatc ttcttcaact
     2521 acttgatctt caccttggtt tccttcgatg aaagcatcag ccatttttga agtaatcaat
     2581 ttaacggcac gaattgcatc gtcgtttgaa gggattacta cgtcgatttc atctggatcg
     2641 cagtttgtat caaccatagc tacgattggg atatttaatt tgtgtgcttc ttgaacagca
     2701 atacgttctt tacgtgggtc aacgatgtac attacatctg ggattcttgg catatcagcg
     2761 ataccgccta agaatttttc aagacgttca cgttctttgt tcaaaccagc aacttctttt
     2821 ttaggtagaa cttcaaaagt tccatcttct tccattgcat tgatttgttt caaacggctg
     2881 atacgttttt ggatagtatc ccagtttgtc aatgttccgc ctaaccaacg atggttaacg
     2941 aagtattgtc ctgcacgagt tgcttcttct ttgattgctt cttgtgcttg tttttttgta
     3001 cctacgaata atgctacgcc gccttcttca gcaacgctct tcatgtaatc ataagctgca
     3061 tctactaatt taactgtttt ttgcaagtca atgatgtaga ttccgtttct ttctgtgaag
     3121 atatatttct tcatttttgg attccagcga cgagtttggt gaccaaagtg tacgccggct
     3181 tctagtaatt gtttcataga aattactgcc atgtttgttt cctccgattt ggttttattt
     3241 ttcctcttct tatcttctac cttgccggca aactttttca agcacctacc gacaatccaa
     3301 taagaatgtg gatttgctga tgcataaagc accctcagct attatacaga caaaagatcc
     3361 gtatcgcaag agcaaatata cttttctgct caacattcaa tagtttacct gaaagataaa
     3421 aagaattcaa gaaaaacagt agatttctct ttgtttcttt attatccttt ttccataaaa
     3481 taattttagg tataaaagac gaatattatt gggcaatcct tctttttttt gttatgatac
     3541 atgtgaaatc tttcgtaaag gagaaagaaa atgcgcaaag ctcaaagaca tttgctgatt
     3601 aaacaagtca ttgaagaatt cccgatccgt acgcaagaag aactattaaa caaacttggg
     3661 cattatggcg ttaccgccac gcaggcaacg atttctcgag atatccgtga tctaaaaatc
     3721 gtgaaggcac ctgatgaaaa tggcgtttcc cgcttcgtat tgtttcgcga aaaaggaact
     3781 cctgagacta ggactgagga agaggatcgg ctggttcaaa tgattgaaga cattgtattg
     3841 aaagtcgaac gagtccactt tttgacgatc gtcaatacct tgccagacaa tgctcattta
     3901 tttgcatctg ttttagatga gatcaaacct ccctctattg tcagtacaat tgctggattc
     3961 gatacgatca tggtcatctc ggaaaatgag gaggacgcaa aacgagtaga agaatttctt
     4021 caccatccaa ctgaagtatc acttttttaa tcagagcacg tattcctaat atggaatgcg
     4081 tgttttttta ttttcaatat tttccttatt aaataaacct ttacactctt tacaaaaata
     4141 catcattttt tattcaattt tttcaacacc ttgattgaat cacaataatc atcatttata
     4201 ttggatgtta acaagagcag cttcttgctc tttgtgtatt tcaaaaaaga aatgaaagga
     4261 aatcatatgt ataaattatt aactcctgga ccattgacga cttctgaaac agtgaaaaat
     4321 gctatgctga tcgatcattg tacttgggat caagaataca aagaaattac acaatggata
     4381 agaaaaaaat tattggatct tgctcaagta tcggaaggaa tatacacaac ggtcttaatg
     4441 caaggtagcg gctcctttgg ggtagaatcg gtattaagtt ctgtgatcag gccagaagat
     4501 actgtactga tttgttcaaa cggcgcatat ggacaacgaa tgatcaacat gtgcgaatgt
     4561 ctgaaactga actataccgt ttatgaggta tcggaacatc aaatcctcca agctcatgtg
     4621 attacacagc tgattgaaga gaatccctct atctccgcac tagcaatggt ccatagcgag
     4681 actacttccg gtattttgaa taatttggaa gagattgctg ctgtcacaca agaaaaagat
     4741 cttttgttta tagtggacgc gatgtcaagc tttggtggga taccgattcg tgtagccgaa
     4801 ctagggattg attttttgat ttccagctca aacaaatgta ttcaaggtgt tccaggcttt
     4861 tcttttgtac ttgctaataa agcggaacta gaaaagcgaa aaaatgtgag tcgaagtttg
     4921 tcacttgatc tatatgacca gtaccgcgtg atggaaaacg agaatggcaa atggcgtttc
     4981 acttccccta cccatacggt tttggctttt caacaagcgc tgaaggaact agaggcagaa
     5041 ggaggaatcg aaactcgata ttcacgttat ctcgccaata atcatgtact acgacaaaaa
     5101 atgacagaga tggggttcca gcctttcatt caagaatatc aggggccatt catcacctct
     5161 tttctttacc ctaaggactg gcgatttgat tttaatcatt tttatcagta tttaaagcaa
     5221 aacggatttg tgatctatcc cgggaaaatc agcgatcatg aatgttttcg tatcggaaat
     5281 attggcgaga tctatccgga agatattttg gaactgacag caattatcaa agactataag
     5341 gagaacgtta atgattaaaa ctgtaatttt tgattgggca ggaacaactg tggactttgg
     5401 ctgtatggct cctgtccacg cttttagaaa tgcttttcta gaaaaaggaa tccaactgac
     5461 tgacaaagaa atccgcgaac cgatgggaaa attaaaatgg gatcatatcc aacagcttct
     5521 ttccctccct tctgtcaaaa aacaatggat agctatttat ggacaactac ctcaggaaaa
     5581 agaggtggag gaattgtatc aacaatttga acattatctg tttaaagatt tggtcaagca
     5641 ttctaccttg aaacccgaca cattggaaac gatcgaacgg ttgagaaacg ctgggatcaa
     5701 tattggttcc acgactggat ttactgctcc tatgatgaca atcgtagcag aaaccgcaaa
     5761 aaaagaaggg tattcaccag acttcatggc aacttctgaa gacgtcgaag ggtatggtcg
     5821 accgtttcct tatatgatcc ataaaaacat gcagtacttc aaaaacaagt cggttgatga
     5881 agtaatcaaa gtcggagata cggtctcaga tatccaggaa gggaaaaatg ccggagtgtt
     5941 gacagtaggt gtaattgaag gtagttcgct aatggggatt tcttatgatg agtaccaaaa
     6001 attagagatg gggaaaaaag atcgtttaaa acagtctgtt gctcttagat tccaacaagc
     6061 tggcgctgat atttgtgtaa acaatttaaa agagttaaca gaatacattt tgtacgctga
     6121 caagaatcca gaatgacttt tatacaaaat cagaggtcgt gaaaaagctg tactgcatca
     6181 agtaataaga acagccaaac aaaaatagct cctcatattt ttcgtttggt taggcttatt
     6241 accgcagatg caagcttttg aacaccgttt attcgaaaaa aaaagggagc tgtgacacga
     6301 cttttgtcac agtcccctta tttacaaaca aacagtgttt aagagacaac ctctccacta
     6361 aacggtgttc attcagctga ctctcggtat ttgctcaaaa aacgataaaa tttgaaaagc
     6421 tattttctct tttgtttctc taatactcgg atcagaacgc tgatttcaca ttctcttttt
     6481 cttacatctt atctggtgca tgaagtccta gcaaacgaag gtcttctttc aataatacag
     6541 tcactgcata aactagagct aagcgtgctt ctttttgttc atcatcagct aaaatcttcg
     6601 tatgagcata gtatttgttg aatgcttgtg ctaatttgat tgcatgtttc gcaatcactg
     6661 acggttcgta tttttcccca gcagacaaga ccgtttcagg gtatttttga actaatttaa
     6721 ccacttccca gctggtatcg tcattcaatg catagtctgc tgtttctgaa gggacaaatc
     6781 ctgctttttc caacaggctg actgcacgag catgtgtata ttgcacataa ggtccagttt
     6841 ctccttcaaa tcgaacaact tcttccaggt taaagtcaaa tgtattcagt cgatcgtttt
     6901 tcaaatcgtg gaaaatgact gccccaaccc caacttgttt agctacagca tctttgtttt
     6961 ccaaatcagg gtttttctcg ctgatttgtt ctttagcaga ttggattgct tcatttaata
     7021 cttcttctaa taagacgatc tttcctttac gagttgataa ttttttacca ccttgcgtga
     7081 tcaaaccaaa tggaatatgg cgcatatcgt cagaccagtc aaagcccatt tccttcaaga
     7141 cagctttcaa ctgtttaaaa tgatagcttt gttcattccc tactacatat agagattgtt
     7201 tgaagtcgta agtacgttta cggtacaatg cagcagctaa gtcacgtgta atgtatagtg
     7261 tcgcaccgtc tgacttctta atcaacgctg gattcaggtc ataagcagat aggtcaacga
     7321 tctctgcacc tttatcctcg ttcagcaaat gtttttcttc taaaagtttg acgatttcat
     7381 ccatcttgtc attgtagaaa gcttcaccgt tcaacgaatc gaaacgtact tccagcagat
     7441 catagatttt attgaattct ttcatcgatt catcacggaa ccattgccat aattgaatag
     7501 cctcttcatc gccttcttcc agacgtttga accaagcacg agcttcttca tttaattctg
     7561 gttctgtttc agctacttca tggaattgga catataaacg aagcaattca ttgattggtt
     7621 ctgctttaac agcttcttct gtaccccatt ttttgtaagc gacgatcagt tttccaaact
     7681 gtgttcccca gtcacctaaa tggttgatgc ggattggctg ataaccgatt ttttccataa
     7741 tgaaaccgat cgaattaccg ataactgtcg aacgcaaatg tcccattgag attggtttgg
     7801 caatattagg agaagacatg tcgattggta ctgttccttg attcccgata ttgctatccc
     7861 cataatgttc tttttctttc actactgttt gcaaaacttt tttgctgata agttctttat
     7921 tcatgaaaaa gttcaagtaa ggtccgacaa cttcgatttt ttcaaagttg gcagaatcga
     7981 ttttttctgc taaatctgca gcgatttgct gtggcgcttt tcggtaaact tttgctaatg
     8041 aaaaggctgg aaatgcaaca tctccgtgtt cagctgattt tggattttct agtaattgtt
     8101 cgacttgttc cagtgttaaa tcatctttga cagcgtcata gacagctttt gcgacaagat
     8161 ctttattatt catctttatt cctcctaaag tgctcccttg attgttaaac aaaaaaaatc
     8221 tctagcgacg gcatacttag tatgccatca ctagagacga gatttcccgc ggtaccactc
     8281 caattgtcct aaaaaaggac cagctccatc tgatatcggt agacgaccgt ctgtttaaaa
     8341 cagaattctc caagtgcgct tcgttgtatc ttcttcattc ggcttccacc acttccgaat
     8401 tcgctagcca agaatgatac aagtactctc ttgttcaaca aatcttagtc ttattaaact
     8461 ttcttgaatt atatcagaaa aaaaaacgaa aggctagtcg tttctgataa aaaaacaatc
     8521 cagatggcct aaaggagaag aaataccacc tggatagcta taaatcattt cgtaaatacg
     8581 aaagacttaa caaattaatc agctacgata atcgttcctt cttcagaagc taaaagattc
     8641 tcgatatttt ctaaagaagt gatgattgcc ttagcgtttg gttttgcttc aacaaatgca
     8701 atagctgcct ctactttcgg aagcatgctt cctggagcaa attgcttttc atcaatgtat
     8761 tgtttcattt ctgaaacagt aacagtttct aatttctttt gatctggttt ttggtaattg
     8821 acatacacat tgtctactcc tgtcaatacg atcagcaaat ccgcatcgat gatttcggct
     8881 aatttttctg aagcaaaatc tttatcgatt acggcttctc gtccttctaa tccagtagct
     8941 gtttctacaa caggaatccc accgccacca acagagactg tgataactcc ttgatcgacc
     9001 agcgtttcga tgacacgtgc ttctttaatc gagatgggtt taggggaagc gactactttt
     9061 ctccatccac gtccagcatc ttctttgaat gtcacagtgg agtcagcatt catttgttct
     9121 ttggcttctt cttccgtgta gaatggaccg actggtttac ttggattttt gaatgaagga
     9181 tcattttcat ccacaataac ttgagtgact aaagaaacca catctttatc gatccctttt
     9241 tccttcagta cttcgcccat ggcattttgt aaccagtaac cgatagatcc ttctgtcatt
     9301 gcaacacatg tatctaaagg cattgccggt gttttctcag aatcagcagc ttgctgctga
     9361 atcaataggt taccgacttg cggaccattt ccgtgggaga tgatcaattc atctccttgt
     9421 tcgataaatt tcactaaata ctttgctgtt tccattaacg cttcttgttg ggcttttgca
     9481 cttgcatccg tagacaagat tgcattaccc cctaaagcga caactacttt tcgtttggcc
     9541 atttcattcg tcctttcttc agttctaccg aattttctag ataacaaaac gagccagcgt
     9601 tttgtgattt tccctcaatg ctgactcgtt ttttcgtgag atgaatgtgg ttatgcttaa
     9661 acgcgaggga taaacaagtt acctaatgtt gctgccatga tcgctttgat tgagtgcata
     9721 cgattttctg cttggtcaaa ttgacgagca tatttgctgc ggaagacttc atctgttact
     9781 tccatttcag tgatgccaaa gcgttctttc atttgttcac cgtaaacagt gtttgtatca
     9841 tggaaagctg gcaagcagtg caagaagatc aaacgatctg tgttgtgtgt tttttctacc
     9901 atttccatat tgatttggta aggtttcaac aatttgatac gttcttcgaa tttatcttct
     9961 tcacccattg atacccatac atcagaataa aggacgtctg ctccgtctac acctttatca
    10021 acatcgtcgg taatcatcaa ttgtgcaccg gatttttcag caaagccttc tgccattttg
    10081 acgatttctt cttcaggttg taattctttt ggtgcaacga tacgcatatt cgctcctaaa
    10141 attgcacctg tgactaataa agagttagcc atattgtttc gaccatctcc gcagtaagca
    10201 acagtgatac cttctactgt tccaaagttt tcttggatag ttaaaaagtc agcgatcatt
    10261 tgtgttggat gccattcatc agtcaagcca ttccaaactg gaacaccaga gaattctgct
    10321 agttcctcta ccattttttg actgaatcca cggaattcga ttccatcaaa catacgacct
    10381 agtactttag ctgtatcttc tgtggattct tttttaccta attggatatc atttgcacct
    10441 agatattcag gatgtgcacc tagatcgatc gctgcagtag taaatgcaga acgagtacgt
    10501 gtagatgttt tttcaaataa aagcgcaata ttttttcctt ctagatagtg atgtgggata
    10561 ccacgttttt tcaagtcttt caagtgttct gaaaaatcga tcaaatattg taattcttct
    10621 tttgtaaagt ctttttctgc taataagctt cttccttgga atactgattc tttcatatta
    10681 aacattctcc tttagattgt ttttatttaa gattaaagag gaaatcccga cggcagctga
    10741 cagtaagacc gccgtcagga aacaaattac ttcaagtctt cacggacaag tggttggctc
    10801 atgcagcgtg gaccaccacg gcctcgtgac aattcacttg aattgatttc taacactttg
    10861 atgccatagc tacgaagcaa ttcgttcgat acataatttc ggttataagt gactacaaca
    10921 cctggagcga ttgccaatgt gtttgatcca tcattccatt gttctcgagg tgcaacgatc
    10981 tcatctccat taccagttgg gatcagtaca agatcatcta atccaagagc agctttcatc
    11041 gtttcgtgta agttatctga atgagtaatc ttgatatcat caccatctgg ttcgattgta
    11101 aagacatcca tcttaccgtc tttactttga attgctggat ggatagtgaa tttatcgtga
    11161 tctaccattg taaatacggt atccaaatgc atcattgcac ggttgtttgg aattttgatt
    11221 gctaacactt tttcaaatcc tgaatttttt gcgaataggt ggcgtgctaa tttttctaaa
    11281 gctttcgcat tagttcgttg agaaatccca acagctacta ctttatcgct taagatcaat
    11341 tcgtctccgc cttcgatatg atctggattt tcacgatcca accatacttc gattccttta
    11401 tttgcaaatc ttggatgatg tttcaaaata tattctgtaa acatcgattc acgacgacgt
    11461 gcttcaaaag tcatcttatt gactgtcatc ccatttccca ttgatgcgga agggtcgcgt
    11521 gtaaagtaca agtttggcat tgggtccatg taaaatggat agtcatcgtc tgctgacaga
    11581 tcgtatagac tgctagaacg tacttcaata tcttttgtac gaaccccagc catgatttta
    11641 tctaccattt cttgcgtttc cattgataga aggaactcat gaagaccctc tcttacagca
    11701 ttcgatgcaa tatgagattc atccaacatt ttattcaaga attgttcttt gacattgcct
    11761 gcgtcaatcg cttctgccgc taatttttcc aagtaaagag tctcgacgcc ttcattttgc
    11821 aatgtctttg caaagttgtc atgttcttct tgagcaatcg gaagatatgg aatatcatcg
    11881 aacaacagac gatccataat atctggtgtc aaattttcca cttcttgtcc aggccttttc
    11941 agtaaaactg tctttaattt ccctatttca gagaaaacgt gaataggttt atccatgttg
    12001 tgttgcctcc ttctttttga ttacatgtat aggatatcct ccttttgtaa tcactttcat
    12061 aattttgtct cattcaaaat gagacattcc tcacattaat attcgataat taaataatta
    12121 aacgtttttc tatttttaag cgtaatttat tcatttttta ttctgtatac actttcaaaa
    12181 aaatatt
//
LOCUS       DACCXO010000084        12062 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.085, whole genome shotgun sequence.
ACCESSION   DACCXO010000084 DACCXO010000000
VERSION     DACCXO010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.085"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..433)
                     /locus_tag="IVY70_002077"
     CDS             complement(<1..433)
                     /locus_tag="IVY70_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP7959195.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIED"
     gene            1127..1513
                     /locus_tag="IVY70_002078"
     CDS             1127..1513
                     /locus_tag="IVY70_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7959196.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1607..1798
                     /locus_tag="IVY70_002079"
     CDS             1607..1798
                     /locus_tag="IVY70_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7959197.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            2312..2644
                     /locus_tag="IVY70_002080"
     CDS             2312..2644
                     /locus_tag="IVY70_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7959198.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2940..3095
                     /locus_tag="IVY70_002081"
     CDS             2940..3095
                     /locus_tag="IVY70_002081"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP7959199.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            3371..4711
                     /locus_tag="IVY70_002082"
                     /pseudo
     CDS             3371..4711
                     /locus_tag="IVY70_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4952..6568)
                     /locus_tag="IVY70_002083"
     CDS             complement(4952..6568)
                     /locus_tag="IVY70_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP7959200.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            6686..6904
                     /locus_tag="IVY70_002084"
     CDS             6686..6904
                     /locus_tag="IVY70_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7959201.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            7099..7560
                     /locus_tag="IVY70_002085"
     CDS             7099..7560
                     /locus_tag="IVY70_002085"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7959202.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(7753..7992)
                     /locus_tag="IVY70_002086"
     CDS             complement(7753..7992)
                     /locus_tag="IVY70_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959203.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(8016..9539)
                     /locus_tag="IVY70_002087"
     CDS             complement(8016..9539)
                     /locus_tag="IVY70_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP7959204.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(9557..9679)
                     /locus_tag="IVY70_002088"
     CDS             complement(9557..9679)
                     /locus_tag="IVY70_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP7959205.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(9709..10692)
                     /locus_tag="IVY70_002089"
     CDS             complement(9709..10692)
                     /locus_tag="IVY70_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP7959206.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(10716..10865)
                     /gene="rpmG"
                     /locus_tag="IVY70_002090"
     CDS             complement(10716..10865)
                     /gene="rpmG"
                     /locus_tag="IVY70_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP7959207.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(10886..11263)
                     /locus_tag="IVY70_002091"
     CDS             complement(10886..11263)
                     /locus_tag="IVY70_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959208.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(11295..11474)
                     /gene="rpmF"
                     /locus_tag="IVY70_002092"
     CDS             complement(11295..11474)
                     /gene="rpmF"
                     /locus_tag="IVY70_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP7959209.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(11489..11758)
                     /gene="rpsN"
                     /locus_tag="IVY70_002093"
     CDS             complement(11489..11758)
                     /gene="rpsN"
                     /locus_tag="IVY70_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP7959210.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
BASE COUNT         3882 a         2293 c         1864 g         4023 t
ORIGIN      
        1 gatcttctat ttttccagta aaaaaataat gaactaaaat cagaatatct cgaatgtctc
       61 ctaaagaata caaagtcaaa ttttgcaatg aattatagag tcgttctcta tcagccattg
      121 caacattcaa attgttcatt tttagtttaa aaaaaacttt atcaatatta tttgctctaa
      181 atggaccaaa taagtaatat ctggttttaa acatgtatag aagaaacatc tcattaaaat
      241 caccagtaaa gaaatggaaa tctttagttt ttttaatatt atcagttagt aactttttat
      301 aatcataaaa aaaataagta gttctatctg agcgatactc ttttataata ctgaattcgg
      361 aattaaaaac cacgataggt aactttaaac aaacatgaag agactttaat aagacttctt
      421 tatctgaaaa cacttttgtt tctctctttc ctttctacat cattaaatag tattttatac
      481 gttatctaaa atactattcc tctgtcaaaa taagctaact tacttcatta taaaatatcc
      541 acttatcgct ttcatgtata ttccaccgta aaaagtacat tttatcaccg agaactcatc
      601 atttttcatc atgatgaata ctatttacat atatctagtt caaagacaat aattattatg
      661 atccgttatt atctaagcac tatacagata ataggaattt ttcgtttcta aatattccaa
      721 tttgggaatc caataaaaat tttcgttcaa atacgtgtct attcgaactc ttttctagtt
      781 gttaattatg aagggggaag aaataaaaaa tacaatctat acacgaataa gaatattatc
      841 caaaaggagg ttttagaaaa gatttattat acgtaaaaag ctcaagacat tttcttagcc
      901 ataaggacat gtttgatatt taagaagata tatgaatttt cttttctaag tataaagata
      961 actatccatt atcaactaaa aaaacatttt tgaaaataaa catcagcagt cctatcaatg
     1021 catgtgagct tatagaagtt attcgcagcg cagcataatt gcattagctt cttttcgtgt
     1081 tttttgcctc ttttcccttt aaatcttaat ggaggaggtg tgcaatatgt cagataacga
     1141 cgaacgcgta aatgcccagt tgattcgatt ctttgaaaag gtcattttga acaccgctaa
     1201 tacttattat aaaaaacaag aagaaattca aaaacatgaa caacttgatc aactcccacc
     1261 atatttgttt actaaagata ctataaaatt taaacaaccg attaatgttt taaatgtgac
     1321 tctccttgtt gaagatataa aattagcaaa aatccttcct ttgttgaaag aacctgaaca
     1381 aatttttctt atcgaaaaat ttatctttga taaaactgat aaagaaattg gaaaggttct
     1441 ggggattaca agacagggtg cgacaaattt aaaacatcga ctttacaaaa aaattcaaaa
     1501 aatgctggtt taatatctgc ataataacta attttgtatg cttctttgct cgttctcctc
     1561 cttcccaaag aaatagatta taagtccatg ggaagaaggt agatttatga attctttaaa
     1621 agaagatttc attttagcaa aggctggaaa tgaagaagct gttgaggcaa ttctcaaaag
     1681 atttagctct ttgattcata agcaatcctg gagaactgga aaatatgatc aagattgtta
     1741 tcaagaatgt atgcttgcaa tatatctagc tatctcgaaa ttcgaaatta aagaataact
     1801 caactaaggg agtctgtgac ataagtaaaa agatagtttc aaatttcgaa caataaattt
     1861 gaaactatct tctttataat tataaagtat cgattttaca gcgttctata atgattcata
     1921 gaatctaaaa cgaatgattt taattatcaa cgttttttaa ttcgttttta gcttaaaaat
     1981 aacttttgtc acagtctcac tcctaaaatt actttattta cgtatgaagt gggcaggagg
     2041 tcgaatccag aactgggcta tggtacttca acgaggactt ctcctctaga attgaaaagc
     2101 atgcgattta ccttccgtaa cgacggtaga tttattcaac tatgtgtcca atcaaaattt
     2161 gtctgatttc aaaaatagtc aagagaattt tggttgttaa aaactcttat ctatcaactt
     2221 ccacagttta gttgacaaac cctgatacta agttgggcta tgatacactt ttcttgacta
     2281 tcagttacgt taccggaagg aatgaactct attgagaaaa acatatgatc gtaaatttaa
     2341 actaaagatt tcacaggata tccttgagaa aaaagtaact accaagaaaa ttgctgaaga
     2401 atacaatatc tctcgtccaa caatttcccg atgggtttca gaatatcgtc gatacgggaa
     2461 aaatgctttt gctggacaag gaaaaagatt acctgataaa gcagatttct acattcttga
     2521 acaggaaaat aaacgactaa aagaggaaga cgagtgttct acgaacacaa agatcgatac
     2581 ggatctccag ggatcactca aaaactttac gatgaaggaa tcgaaatcaa taaacgtgtt
     2641 gtagctgttc ttatgcgtga aatgaattta tgcacaagag gatttcatcg acgcaaatct
     2701 tctcatggta gagaaaaaac aatcaaagaa atggtaaaaa aatgttgaat cgataatttg
     2761 aacagaacca aattgatgct gtttgggtga tggatattac ttacattcca tgtaatgacg
     2821 gtcgcctcta tttgtccacc tatattgact tagcaactcg tattctacgt tactatatgg
     2881 tagattcaca catgaaaaaa gagattgtcg tccgtccatt acaaatttat taaggagaat
     2941 tgcctacggt aattcattcc gatagaggta gccagcatcg gtcttttatt tatcaagagc
     3001 tgttgaacga ccaccacatc acacattcag tgagccaaac tgggactcct gttgacaatg
     3061 cagtaattga agcgtttcat cgatcaatca aatgagaatt aatcgactcc aacaaatata
     3121 aaagtaaaat agagatgaag gtgttaatcc aagattattt agaagactac tacccaaaca
     3181 aaagaatcca tacaaaattt atgatgactc cttgacaatt tgaagcgaag aaaataaggg
     3241 atctataata tctagtgttg gtgacgcctt ctgttgccaa tactttttct tttttctaat
     3301 aaacgcaaac agacacctta aaatccttta gaataaagtc gacgaaaacc attcaaagga
     3361 gtgtttcaag atgtcctatt cagagtttac caaagagtta ctagatattc tagacctaaa
     3421 tcttatcttt catgaagatg cctttcgcaa agagcgaatc aatgacgaaa catgttttgt
     3481 ttttgatggc acattgacct atcaaccgga ggaatgcttt catcgccact atcaaaatga
     3541 acaaacgatc attaaatagg gctggaaaaa agtatccatc ttattgaatg atgtcagtaa
     3601 ctacaagacc attctgcgga ttaacaaaca gcgcttcaaa tgtaaacact gtggaaagac
     3661 ttttttagca gaagattcag ttagtgatcg tcgctgctcc attgctcgca gagtcaaaca
     3721 agccattctt gagctattaa gcgaacctct ttctatgtct ttgatcgcac gtatgaagca
     3781 catttctccg accactgtca ttcgaatact tcgcagcctt cggcctaaaa ccatttcttt
     3841 gagccagctg ttaccagaag tggtgtgttt cgatgaattc aaatcagtga aaaatgtctc
     3901 gggagccatg agctttgtca tgatggacgg caagacccat caactgattg atattgtgga
     3961 aaatcggcaa ctcaatcctt tacgcgacta tttcttgcgc taccctataa aagtccgtga
     4021 acaagtgcgt ctagtggttt cagatttcta tacaccctat cgcacattgg tgaaagagtg
     4081 ttttccgaat gcccgaatcg tcgccgatcg ctttcatatt agccaacaca ttggtcgggc
     4141 ttttaccaac caccgaattc aagtaatgaa gacctttaaa aaaggggatc gtcggtcgaa
     4201 gcacttaaag aaatactgga aactacttca aaagaatgcg tgggaattga aaggtcagca
     4261 tcgctactgg cgtccgtcat ttcgagatca tctgacagaa gcagagatag tcgatcggct
     4321 attatcttat gatgattcct taaagcgagg ctacgaagtc taccaggatt ttctttccgt
     4381 tatcagaaga caagatgttc ctgaattcgt cgcgctccta aaagaagact acaaggagtt
     4441 gccagagcac taccaaccag tgttcacgac cttcaagaaa taccaaacag aaatcaaacg
     4501 agcgttgcgc gttccctatt ccaacggccc tatagaatgt ttgaataacc atatcaaagt
     4561 actaaaacga attgcctatg gctatagaaa ctttcaaaac taccgagaac gaatcttttt
     4621 atatcgagga aaatatttta agaaaactaa gaatactacc caactgacta aagccagaac
     4681 aacaacacgg ctagacaaga tagctgtcta gccgtgtgag tatctcgatt attctattga
     4741 atttcttcac caacactcat tgacgaagag ccaaaataag taatctaacc gactgttaac
     4801 taaaccattg caggtcccgt taaagatact tgtgcctgtt gtcaccgctt ctttttcttg
     4861 tccacaagcg cttaacacac ttaataaagc ccctgctatt attaataata atatcttttt
     4921 cttattaaaa acctcctcat ctatttccag attagtaaat gtagtaatct gtgctttctt
     4981 ttttctcaat actaataact aacttatggg gtaaacaaat actcgtttga ccgggttttg
     5041 agatccatcc agttctaacc gcgatttgat caggactatt atcttcttta actcgaattc
     5101 ttccatcttt aatctcaaca atattatatt gtcccttcgc aggatgataa ttcacgagtt
     5161 tatgattgat cttatccaaa tcgaagcgat ccatttcctt gccatcaatt cgaacgatgg
     5221 catatttgat agcatcttca gttgtttcat tctccttttc tatagagaat acaaataagg
     5281 gaataaaaga ggtaataatc aaaatgccaa taattatgac atcgcctatc ttgacaccag
     5341 cttttcttaa aaatttcatt aacgatatct ggctaaacat attagacttt ttccatggaa
     5401 atggtgaaat ttttcggaaa ttatctgcat taagagaatc agttaaacaa ccctcaagtt
     5461 gttccttaat acattgttga tccatcttca taccaataaa aactagttta ttaatgcgtt
     5521 caccaaacaa cggatgccag gcttccttca attcgggatt tttttgtata atcatttgac
     5581 actcaatttt aggtaaacta gctacccaat aatttaatga ttcaatactt ttatagctac
     5641 ccgatgtttc aaagatcgtc gaaaaatcac tatgttgggc aaaccaaaga aacccttttg
     5701 ctcttgtaat attgccagga aaatttttat ccgtaagatg ggcaaatttt tgtgcgtcaa
     5761 ggagtaaccg atttcgatag ataaaacttg agataccata ttcttctgtt tcaggaatat
     5821 gattttgata gccaagctct aactctttga accagcccac tgaataggaa gtctcatcta
     5881 tattaaaaag atttgtattt agaattttcg ataagtctac ttcaccttta attgtcctaa
     5941 ttattttggc attggactgt aacttagcaa ttagaccgtt aattttgtaa atcgtcttgg
     6001 ttgacactaa gtcactatta tttaaaagta atatgttaca acactcgaat tgatcaatta
     6061 acaagccacg aatatcctgt tcttcttcaa ctacattacc ttcagctttt ctttcctgaa
     6121 tcttttcacc cgtttcgaaa ttatcccaaa aacgttttac atccactact ataataatgg
     6181 caccaatctc taatcgttgg gtcaaatcaa tgtctaaagg gtttttagct aatataattg
     6241 cttgaataat tggaattggt ttgctgattc ctgaagcttc aataacaatt tgattcaagt
     6301 cagaaaattg cactaattga tctagttcaa caatcaaatc ttcgcgtaat gtacaacaaa
     6361 tgcatccatt cggcatcgaa acaagttttt ctccagttct tttaaaacct ttcttgtcga
     6421 ctaatacccc atcaacattt acttccccta tgtcatttac aatgacagca atttcctttc
     6481 cgtgatcacc gtacagaata tgatttagaa gtatcgtttt tcctgaacca agatagccag
     6541 ttaagattgt aataggtatt tttttcatag ggaacctctt tctatacctt gaatttacac
     6601 tttaagtgca acacataaaa atgagtcata atcgatatcc aactagtata agtttgtcga
     6661 gaacactcct agaaggaaaa taattatgag ccagtatcgt catcttacta tactcgaacg
     6721 agaaagaatc tttctgcttc acgaagaagg attttctatt agtataattg gcaacgacat
     6781 tcagcatagc ccgtttacta ttttacatga actgagaaga aacaagaaaa aaactattca
     6841 ccttccaaag cggaaaaaaa ttatgcttat cgaagaaaaa aatgcggacg ttatcacatt
     6901 ctaaatgatc cgtgagtttg gtcaattgtt cgtcatttat ttgtcgattt tcagtggtcg
     6961 ccagaggaaa tctcgaatcg tttgaagcat gaacatatgg acttttcaat tagttttatc
     7021 acgatctaac gtgcaatttg caatgatctt ttcgatacag aacccctctc acacggcaat
     7081 aaaggcctga tccgttcatt gcaccatcga ggaaaaacaa gacatacaaa aaatcatgtc
     7141 gagtctcgtg gaaagatttg ccttactcat tcgatccatg gccgtccaaa atccgcagat
     7201 gatagaagtg attttggtca ttggaaagcc gatattgtag ctggcaaaac tggtggtact
     7261 tgtttagtga ctttgacaga cagaaaatca tgcttccttt tagcaggaaa gaaggtcccc
     7321 agaaagaaag ccgcttatgt gagcgagaaa atgattgaac ttttaagcag tattccaaat
     7381 gaggcggtca aaacgatcac tccagataga ggaaaagaat tctccaatca tttcgcagtt
     7441 acagaagcat tgaataatat gcccttttat tttccagatc ctcatgccct tggcaacaat
     7501 gaacaaatta aaatacgaat agactcattc gagaatactt gccaaaatcg aaagagatag
     7561 ctacggtaga taactcaacg ataaatcaga tgattgaaaa actgaataac agacctagaa
     7621 agtgtttaga ttggagaaca ccttatgaag tcttctttga taaagtgttg cgcttgattt
     7681 gacaattcaa gacataataa aaagcagatc aaatccaaat aagggaaaaa tgatctactc
     7741 tggtattatc aattaattgg ccaagctatt atgccgtttt gctaaacgtc tacttcttcc
     7801 agtattgacc ttgataaaac aacaatcagg atcccgtttt aatactctat gaactgtgtc
     7861 aatactaaca tgtaaagttg cagcaatatc agctaatgta ctcccattta atctcatttg
     7921 ctttgcaaat cgataacgcc gataagcatc aatattcttg gaatccatag acaataatac
     7981 ttttcttacc atgtatttcc tccaaaaatt gaattttaat gagccatcat ttcttgagca
     8041 atttcatgtt tagacaatga agctggatag aaagtaggcc aattatgcaa ttcattatag
     8101 agtttttctt gactttctcc accaaagaaa atatggaaat gctctgcctt acttggtgca
     8161 attccatgat cactaaattg aatatattta aaacgaccag cacctgcatc atcagtttca
     8221 aaattaaaac gaacaccacg gtttcctttt tcataattca aaattttgta tccaacatat
     8281 ttgtatgtgt attgatgatg ttcaccgttg actacaaaat caatggtgtt gtctttaatg
     8341 ttgatagtat caatatctgt cttatagcca gtagtataat aatctttgta ctcggcagct
     8401 gtcatgtctt tattgatttt tgctttgtaa tcgaaaactt catccaaaat tccatctttt
     8461 aacaacggat aaacagattg ccattcgcct gtataatcac tcaattcgcg atccttcaca
     8521 tcggcatctt taaagtaccc attagcaact gtcttttcat ccttcgcttc tttttctgga
     8581 actacctctt caccagcaac catagttgtt ttttctaacg cttttagatt gtcttccatt
     8641 actgaaacat aatcttcacc attatccatt tgttcttttg taagactttc aagaggattc
     8701 agtacttcca attttatccc agcttcgttg gccaaagttt ttgcaatttt atcattagca
     8761 ttcttttcaa agtaaatata attgatctta ttttttttaa catactcttt taattcagct
     8821 aatcgaccag aagatggctc ttcttcagga cttagtccag caataggaac ttgtttcaat
     8881 ccataatcca aagctaaata tccaaaggct gcgtgttgcg tcacaaaact tttttgtttc
     8941 gcttctttaa gactagtggt atagtcagca tccaaccgtt tcagtttagt caaatacttt
     9001 tcagcatttg tctcaaatac ttttgctttt ttcggataca atttaactaa ctgatccttg
     9061 atatttgaaa cttccttaat tgccattttg ggtgaaaccc acgtatgagg atctagttca
     9121 tgatggtgat cttcttctcc gtgatcatga tcttcttctt cactacctgg caataaaatc
     9181 atgtcttttg ttccttctac tacattaggc ccagctttct tccaactttc tttagctttt
     9241 ggtacccatg attccatgtt ttcgttatga tatacaaaaa catcagcatc atgagctttt
     9301 gccatatcct tagctgaggg ctcatagtca tgaggttctg atcccgctgg aaccattaaa
     9361 tccacgttcg cttcatcgcc gacgatgttt ttggtgaaat catacattgg atagaatgtt
     9421 gttacaattt ctaattttcc atctttatca ttcgatactt ttgcatcatt agtgctggag
     9481 caagctccca ataatcctag agctgctatc ccaataagtc ctacactgat tttcttcatt
     9541 ctttttcctt ccttaattat gctaatttct tcattttatt tcgtttatgt tctttgataa
     9601 tttttaatgc cctttttcta gttttaatat tcgggctttt aagtcgtcga tatgcactag
     9661 atatatccgt tttctccatt agaaattacc cccaatcact acttatcatt atttgctttc
     9721 cgccagtaat ttatcaaaac tctgtttgat cctttcttca tccaacattt ttccgattag
     9781 aataatagtg gtttggtctt tttgtcgagt ggtgtattga aaattaacct gttcgtttac
     9841 ctcttgcatg gcaactataa aatcctgctc ttgtaaagca atcaatcctt taaaacgata
     9901 caggctccct tggttggtta aaatcagcca gtcaatccat tggagtaaaa aatctttttt
     9961 tatgtcttga tctgaagtta agagaattga tttaaactca tgtacttgat ggtgttgatg
    10021 atagtatttg ccattatagt gattttctga tgaatcactt tcaaaaacta agggatgatt
    10081 aaattttttc aaattataaa aatccttttt ttgaatcgtt tcactatgtg tgaacattcg
    10141 aatatcggat aaagggttaa tacttttgat ttctctctgt aattttttta gtgcagataa
    10201 attcaagttt tgttttctag acacaaaaat gcgatctgcc atgacaagtt gcttaagggc
    10261 ttgtggttct ctttcaataa gttcaaaact gtcactatca ataacagtca ataaactatc
    10321 gatatatact cttcctctta gttgaggcgc tgttaaaagt gtttgaatga ttggttgggg
    10381 atctgctata cctgtagttt caatgatgat ttgtgaaatt ggttgttgat gttcttctat
    10441 tgtttccagt aaagaaatca atattgctaa taaatcactg cgaagactgc aacacataca
    10501 tccattattt agttgtaaca cccgttcctc gctatgaact aataatttgt gatcaatccc
    10561 aacttcacca aactcatttt caattataat aatttcttcc ctggggtagg gtgagacttg
    10621 caaggcttga ttgattagcg ttgtttttcc agcccctaaa aacccagaaa cgattgtaac
    10681 tggtatgccc attttgtcac ctttttacca cttagctatt tcacttcttt aaaaattgcc
    10741 cgtctgcgca attttggcga atactttttt aattctaacc gttcagggtt attccgttta
    10801 tttttgctgg tgagatacag acgttctcca gtttcgacac attccaaaat aatattttga
    10861 cgcataaatc ttcctccttc aatacttatc gatcttcaag atatgcttga atttgttgtg
    10921 catcttcaaa agttaactcg ccagattcca attgtgaaac taaattttct ttcatatcaa
    10981 caggtatctc ccaataacca tcgatacatt gaaataactc ctcgtcatac tcttcaaaaa
    11041 ttggatagat tcttaaaaaa tcatcaaatg ataagccttg gtcctcatag taatctttga
    11101 ctaatccatt ttcaattgcc tgttgggtcg gtaaacccgt ttcagtaata attcctctct
    11161 ctaccatcga acgataaaaa gtgcatttat ctagcgattg ctgggtcgtt tcaagataat
    11221 actttaatcc ttcttcaact aaaggtgggt aaataatttc cattttgatt ccctcctaaa
    11281 agttatatat taaactattt tgaagctcct ttaattactt tctttccatt atagtaccct
    11341 tttaaagata catgatggct acgtctataa tcaccattcg cttcgtcaaa tgaaatttct
    11401 ggttttacta cagtttgatg agttcttctt aaccgtttct tcgccttaga tgtttttctt
    11461 gctggtactg ccatgatatt tcctcctttt accaacttgc tttttttact ccaggaatca
    11521 acccttgatg ggcaagttct ctaaacttaa tacgggacat accgaacttt cgcatatacc
    11581 ctcttggacg accatccgtc tgatcacgaa gcctcagacg attcggattt gaatctcttg
    11641 gcaatttagc gagagcggtg cgatctcctg cggcttttaa ttcctgacgt ttatctgcat
    11701 acttttcaat catcgccatt tgtttctttg ctttagcaat tttcgacttt tttgccatat
    11761 acctttatac tccttttatt ttgatttaga cacctcaaat cgtaatcgtt acgttttgac
    11821 actgatactt taccaacaag agttgctctt gtcaacaata aataacatga aataaaactc
    11881 atatgaatag ataactattt gaaaacgaat ttatcggtac atagagataa tcaaatcatt
    11941 taaaagaata tgcagtttag gctctgaaaa ataagaattc acttttatta gtccaatcaa
    12001 acacaaatac cgttttaagt agcttttcct ctacttaaaa cggcaatatg aaagaaacta
    12061 ta
//
LOCUS       DACCXO010000085        11918 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.086, whole genome shotgun sequence.
ACCESSION   DACCXO010000085 DACCXO010000000
VERSION     DACCXO010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.086"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(143..1345)
                     /locus_tag="IVY70_002094"
     CDS             complement(143..1345)
                     /locus_tag="IVY70_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7959211.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1348..1542)
                     /locus_tag="IVY70_002095"
     CDS             complement(1348..1542)
                     /locus_tag="IVY70_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP7959212.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1589..2437)
                     /locus_tag="IVY70_002096"
     CDS             complement(1589..2437)
                     /locus_tag="IVY70_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HAP7959213.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2642..2992)
                     /locus_tag="IVY70_002097"
     CDS             complement(2642..2992)
                     /locus_tag="IVY70_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959214.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3587..4033
                     /locus_tag="IVY70_002098"
     CDS             3587..4033
                     /locus_tag="IVY70_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP7959215.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4657..5649
                     /locus_tag="IVY70_002099"
     CDS             4657..5649
                     /locus_tag="IVY70_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7959216.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5665..6549
                     /locus_tag="IVY70_002100"
     CDS             5665..6549
                     /locus_tag="IVY70_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7959217.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6598..8205
                     /locus_tag="IVY70_002101"
     CDS             6598..8205
                     /locus_tag="IVY70_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7959218.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8206..9690
                     /locus_tag="IVY70_002102"
     CDS             8206..9690
                     /locus_tag="IVY70_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP7959219.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9867..10859
                     /locus_tag="IVY70_002103"
     CDS             9867..10859
                     /locus_tag="IVY70_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7959220.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10911..11780
                     /locus_tag="IVY70_002104"
     CDS             10911..11780
                     /locus_tag="IVY70_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7959221.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
BASE COUNT         3700 a         1946 c         2466 g         3806 t
ORIGIN      
        1 ttgaaaaacg ttgatttatc ggcattcccc atccatatta tatttacgtc ttattcccac
       61 tcaatatgag tagcctcgta aagaagctat tatatcagtg ctttttcagc ctattcctca
      121 ttttggtagg gaaatggtag ggttagattt ttactgcttg atttccatta aagtttgcaa
      181 ctttagattt tgttgcacag gtaaatttaa tgtttcctgt catttgttct gcaattcctt
      241 catcattacg gctccataga tgagcgtagt gctttaaagt gatttcaggg ctagaatggc
      301 ctaaccgttg cgacacgacc agaatgtctg cgttaaactc attgattagg tagctaacat
      361 gagaatgacg taatcctttt ccttgtatta ggggtacctt agctagtttg gcataacgct
      421 gaataattcg ttgaacagtt gaacgataga gtgggaggtc agtgtagcta agaataaatt
      481 gttctacacc atgatttttt tgaataactt tccacttttt tagcacttca attgtatcat
      541 cgtccagaga aatgatccgc aagccgcttt cagtttttgt gtaaggtttc cgaataaaat
      601 ctttttgatt tgtcatttcc aaggtgtgat gaaccctcaa ctttttacgt ttgaagtcca
      661 cgtcattcca attaagtgct aatccttcgc ttacacgaac accagtcatg taatatagcc
      721 agatcattaa aaaacacata tgttcgtaga aatcatcttt ataaattttt gccaaaactt
      781 cttcaaattc ttcttttgtc cagtaaggaa cgattgattt tccttttgga attgcttttg
      841 ttcttttgga aatattctca ttcagaaatt gcagggtgac cgcatagtct aatgatttac
      901 gaaacatacc gtacataagt gaactgtatg cttgtgagta tccgctatcg ttcaataacc
      961 aaattcgata attttcacaa tctcgcacat tgatttcacg aagcttctta tcaccgaaaa
     1021 attcagtaat ctgaatcaat ccacactcac gagaactcca agtacttttt tgtacgctag
     1081 ctttgtagtg tggaacgtaa gactgctcca taaattcttt gtaagttaaa tcataattcg
     1141 caaatccatt tacaaccatg tattcatttt ttatacgcat gacttctttg tgtgcttcac
     1201 gagctgatga gaatcgtttt cctagagaat cttttgttcc tttcttctgg atcctctttc
     1261 cagtcacttg atctgttcct aactcaacta gataagaaaa gcgaccattt ttatcttgat
     1321 acacacaagg gtattttgtt ttcatagtta ttcctcctgt gaaagaggaa cacctataat
     1381 ttcttcaatt acttgtcggg gtactacccc aactctttta ttattataat atggatatcc
     1441 ctgatttacc attcttgttt ttgcttgtcg gattattgtg gtagcttgat gtgttttaaa
     1501 tcctagagcc tctaaatctt ttcgacaaat tgtatttttc attgtataaa ctcactttca
     1561 aagaaaacac tatcattggg taatagtgtt atttctttat ttttgtataa tgaacggtac
     1621 tttcttgaaa atccatcact tgttcaggac ttcccaactc atttaaagct aaagcattgt
     1681 aaaaaaatgg acgtctaact atatattcgc ttgtatatag tatatcttga ttcgtggaat
     1741 catagggttt ctttaccatc tttttggtga cagttggtag tttgagattc cgggacttga
     1801 aaaaagcttt tttcttatgc tctttcaatt ccagctcttt atcgaaatat ttgctgatat
     1861 agcgcccgcg attttcgata ctgtctacat cgatcttgtt gatcttaacg aaaccctgac
     1921 cccaaatcgt agttaaccgg ttaaatgaga taaaggggaa atcaaacaag ataatatggt
     1981 agtggattgc accgcgttgc tgcttttccc acgtggcgag atactttaaa tttgcttttt
     2041 tagttttata gaggtcataa ttcagtcgtt tgataaattt tttaaattca ttatttgcat
     2101 actcaatgtc ttgaatgttt tctttgaaag tgagagttaa aaatttcgtt tgattgtcaa
     2161 aattcgtgtc tacaaggcgg gcaatctcaa atcgcatact ttgataatgt ttctgtttgc
     2221 gttttaaact gtcatattgt ccttgagcgg ataattcatc atagtttttt cttttttcaa
     2281 gagtaatatc attttcataa atccaagcta atttttgttt tcgttccaga tttacttcgt
     2341 tttcgatcgt tttacgtttg ggagtaatga ttgcttcata atcataaatt tcaatatacg
     2401 tgggagtttc aatgattttc tgattataag cttgcaaaaa tcatcactcc ttgttgcgta
     2461 ttcgattgtc aaagaaccag tcattttctc ctgatgttga tctataaatc aagttaaggg
     2521 ggaaggcctg cgtagcagcc cttctagcct atggggtgtt gggcggtctt aacggacaga
     2581 aacggctctg tgggcgctgc cccgccgttt cgtcctaacc acccaacact ccatagtata
     2641 gttagttttg tgtagtggta ccttgttttc tgggaatcaa agttgccgta tcagcggttg
     2701 ccgataaccc ataactgaca aattgtccat tcgagcgtcc ccaaggtaca atcaccaaat
     2761 tggtaaatgt aattatggct aaaccttgtt gggtaagttc ttccggcgtg atcacaggat
     2821 gttcagattt gactttgaca ctgatttttt cgtagcgaag ttttggtagg atacattcgt
     2881 agctccaacc tagaatttcg ccctcctttt gccagcgagt gacattgacg acttggtatt
     2941 ctttttcgag tgtttctttt ggtaaactga gatcggtaat ttttattggc atcacaattc
     3001 tcctttttta tacgaaagtt tatacgattt gattaaaaaa tatagatggc catctacgac
     3061 ttgtattata tacgagaact tatacgtagt caagagattt tctaaaaaaa tatatacgat
     3121 tttttatacg agtgttataa tgaaaaaaat gataaggagg aatttgaata tgtttggttt
     3181 agtacaacct aataaagtga aggtgggcca acgaatcaaa gaaataaaag acggaatgtc
     3241 tctctctttt acagaattag ggaatcgatt aggtttaaaa aagccgacga tcagttccta
     3301 cgttcaaggc tatgcgttgg ctcctaagag tgtgatcaac caattatcta gtatcagcgg
     3361 aaaacctgtc ggctggtttt attttgggca tgtggaagaa tatatccgag agtatttgtt
     3421 tctcattggt cagaaaaaaa tagtggacga ttatcctgaa atcattgaga aaataaagca
     3481 agaattttat acgggcgagt tcaaaaatcc tgcttgggag aatgagctag gctatccttt
     3541 ggaggaattt atccaagaat gttttgtaga atattcgagt gaaatcatga aggactatgt
     3601 cgtacagatt acgaaagagg tacttgaaga atccaaaacg ctaaacaaac tatcatcaaa
     3661 agcaaaagaa gaagctgtca cgattctttc atctgggatc attgactata tcgagatgag
     3721 tggtgagatc aagtatggag agaaagatcg cattgcgaaa atggtgaaag aatcaataaa
     3781 aacctttgat gtgaaacaag acctgacatt taatgattcc tatttgattg gaaagttgat
     3841 caatattctt ggtacagatg aagatacgga gctgtttctt tcttctttat caatgatatt
     3901 tactgataag catttttcca ctcgatttgg tggggaagaa ttagtcgaaa tatttcaatc
     3961 gatgcgacca gcgttaatca aactctatcg ggagaaaaca ccagatgaat tttatgattg
     4021 gtttgagaag taaaataaga aaagtggctt agattacaag ttggtgaaaa aatttttttc
     4081 agcataacat cagtaatcta taaaggggtt gttgattata ctgtagaagt taagtactca
     4141 agagattttt tgtacgtaaa caaagcaaaa aagggtgcag tttcactaga aaatgtactc
     4201 ttttttgtga tttttcttag ttatagagtt tttttgaaac tttaaaaatg agaaaacata
     4261 ttgattttaa agagtgtaga catagcttct tttgctaaat agattaaact tactggtgag
     4321 tggtcttgaa catactttta aatcacattt tactgagttt tattggattt tataattcat
     4381 tggaaacctc gtacttaatc tgtttttatc tatcgactag aatctgtata tattccagct
     4441 atcaatgagg ccgcagaaaa gggtgcttaa aaataatctg ttgtttaaaa tattattatc
     4501 agaatactta gatacaaaat tctggcacaa agtaaaaaaa atattgactt aaaaaaatgg
     4561 atatggtaat ctataaacga aaatagtggt aacgtttcca aaatgcatct tcgttaaatt
     4621 tttatttttt taaacgaaaa tggaaacgtt tctaaaatgt attgggagga gattgaattg
     4681 gaaaagacat tgggaccagg tggtgagata agggcggtac aacgggataa aaaaaatatt
     4741 tcgaagtggg aaaaattaaa gaaacagttg cggcgtgata agtggatgta cttgttgctc
     4801 ttaccaggta tcatcatgac gtttatcttt aagtatgtgc caatgtacgg tgctgtgatt
     4861 gcatttaaag actataatcc tatgctagga attttggaaa gtccttgggt aggactagat
     4921 cactttaggg aatttatcag ttcaccaaat tttacgcttt tgctgagcaa cacattaaaa
     4981 attagtattt atggtttatt gttaggtttt tttccaccaa ttattttagc actgagcttg
     5041 aatcaaatta tcaataaaaa aataaaacag aaaatccagt tgattctata tgcgccgaat
     5101 tttatctcag tagttattat tgtggggatg ctgtttttgt tcctatccgc aaatggacct
     5161 attaatgggt tagtagaaaa ggttacgggg caaccaatta tgttcatgtc taatccagct
     5221 tatttcagaa cgatttatat tttatcggga atttggcaag gaatcggctg gtcatccatt
     5281 ctttacaccg caacgctggc gggagtcagt accgatttat atgaggcagc aaatatcgat
     5341 ggggcaaata ttttacaaaa gatttggcat atcgatttgc ctgcattgag gcctgtgatg
     5401 gtgatcagct tcatcttggc cgccggcggt attatgaatg taggatatga aaaagcctac
     5461 ttgatgcaaa cttcaatgaa catccctgcc tcagaaatca tttccactta tgtatacaaa
     5521 gtcggcttgc aatcaggtga ttatgcctac tctgctgctg ttggtctgtt caatatgatt
     5581 attaatgtcg ttttattact gattgtcaac aatataacca agcggctaaa cgatggtcaa
     5641 ggcttgtaat gaaaggagaa aattatgaat agtgctttat caaaaatcga ccgtcaaatc
     5701 ttatggttga atcgagtgat tgtaactttt ttagtcgtca tcaccattgt tccgctgatt
     5761 tatgtactgg tagcttcttt tatggaccca gctactttga tcaataaagg tataagcttc
     5821 aatccgaaag attggacaat acagggatat caacgggtat ttgcagatga ttccatttta
     5881 cgcggttttg taaattcgtt gttttactct tttagcttca gcttattgac tgtatttatt
     5941 acgatgatca ctgcgtatcc tttgtctcga cctaacttgg cggggaaaaa gattattatg
     6001 actttttttg tcattacaat gttcgtgggt ggtggcttga tacctaccta tttattggta
     6061 aagaacttgg gcatgttaga tagtgtatgg gcgattatcg taccaggtgc tataaatgtg
     6121 tggaatatta tcttagcccg tacctatttt agtgctttgc caaaagaact ttctgaagct
     6181 gctactatcg atggcgccaa tgacttgcaa atactgttca aagtcatgct tccattagcg
     6241 aaaccaatta tgttcgtatt gtttttatat gcctttgttg ggcaatggaa ctcatacttt
     6301 gatgccatga tttacatcaa agatcccgac ttacagccgt tgcaactcgt attaagaaat
     6361 attttgatcc aaaaccaacc ttcacaagac atggtgggag ccaatactgc catggcagaa
     6421 atgaaacaaa ttgctgagtt gatcaaatac gcaacgatcg tcatttctag tctgccatta
     6481 atcataatgt atcctttctt ccaaaaatat tttgataaag gtgttttagt tggttctgtt
     6541 aaagggtaag tattgttcac tatttgtatg aataaaaatt ataaaggaga ttttaagatg
     6601 aaagcaaaat ggatgctgcc aatcgttttt acttccgtac taattttggg ggcttgtgga
     6661 ggctcaaaag aaaagagtaa atcaagccct gattatgaac tagaaaatgt ctcttttcca
     6721 ttgaaagaaa aagtatcttt aaaaatgatg tctcaaagtg cccctttggc tcccaatgat
     6781 cctaatgaca aattgatttt ccaacgatta gaagaagaga ccaatctcca tattgattgg
     6841 acaaactaca gtagtgattt tgccgaaaaa agaaatttgg atatcgctag tggtgattta
     6901 ccagatgcca tcttcaacgc cgctgcaggt gactatgatt tattaaactg ggcggaaagt
     6961 ggcgttattg taccgattga agatttgatc gaagattata tgccaaatct gaagaaaatt
     7021 tttgatgaaa accccgaata ccgacaaatg tctactgcac ctgatggtca catttactct
     7081 atgccttgga ttgaagaatt aggcgaagga aaagagtcga ttcatacagt aaatgggatg
     7141 gcttggatca acaaggagtg gttggataat ttgggtcttg aaatgccgca aaccactgat
     7201 cagttgatga ctgtgttgga ggctttcaag acgcaagatc caaatggtaa tggtgaagca
     7261 gatgaaattc cttttagctt tattaacaat ggaggaaacg aagacttaaa atttttaatg
     7321 ggggcattcg gtctaggtga taatgatgac cacttagtcg ttgataacga tggtcaggta
     7381 caatttactg ctaacaaaga agggtataaa gaagccatca aatatttcaa tgaaatgaat
     7441 aataaaggtc ttattgataa agaggctttc gaacaagatt ggaatacgta tgttgccaaa
     7501 gggaaagaac ataagtatgg cgtgtacttt acttgggata aagcaaatat cacgggtgca
     7561 gatgatagtt atgacgtctt gccagtctta gcgggaccag atggaacgaa acatgttact
     7621 cgtacgaatg gcatgggttt ctcaagagat cgtttcgtaa ttactagtgc gaataaaaac
     7681 ttggaactta cagctaggtg gatagataaa atgtatgaac cgttacagtc tgtccaaaat
     7741 aactggggaa cctacggtga tgaagcacag gcgaatattt ttgaattaaa ggatggtatg
     7801 ttaaaacatt tgcctttaga ggggacagca ccgggggaac taagacaaaa aaccgaagct
     7861 ggaggaccat tagcagttct tgattcttat tacggcacag tcactactat gccagatgat
     7921 gcgaagtggc gtttagattt gatgcatgac acgtatttgc catatattac agaagacaat
     7981 atctatccac gtgtcttttt agaaaaagaa gaaacagatc gattagcaaa aatcgaaacg
     8041 gatatgtggg attacatcta tcgcaaaaga gcagagtgga tcaccggcgg taaaatcgat
     8101 gaagaatggg aagaatacct tgctgaattg gaacgtgtag gtttggaaga atggattcaa
     8161 ataaaacaaa ctggctatga taattttttg aagggagcac aataagtgtt agtagaagaa
     8221 acggcgattt tggataaagc aaatcgattt attgaaatga tgcgaggaaa agttaaccct
     8281 cagtatcgac aaaactatca tttttccgca cccatcgggt ggataaatga ccccaatggg
     8341 tttatatatt acaaagggca ttaccatctc ttctatcaat actatcctta cgatagtcga
     8401 tgggggccca tgcattgggg gcatgcgcgc agtagtgatt tggtccattg ggaagatcta
     8461 ccagtagcgt tagccccaga tcaaccttat gacagcggcg ggtgtttctc aggaagtgca
     8521 attgaaagag acggaaaact atatttattt tatacaggct gtcaagaaat tgatggtatt
     8581 agccatcagc atcaatgttt ggcggtctct gaagatggta ttcattttag caaatacagt
     8641 ggtaatcctg tggtagcaga aaaacagcta ggtgaaaatg ggctaataca agaatttcgt
     8701 gatcctaaag taattgttca caacgatggt tactatatgg tagtagctac taaatctaca
     8761 cagtctactg gtagaatagt gatgtttcaa tcgtcagatt tgtatacttg gcacttttac
     8821 tctgtgctgc tagaagggac ttcagagcaa ggaataatgt gggaatgtcc tgatttattc
     8881 catttagacg gacgagatgt cttaatcatg tctccgatac aaatcaagaa gaacggactg
     8941 gaatatcata atatcagttc taccatggct tgtattggtg aggtaaactg ggaaacaggt
     9001 cagatgatag ttgataattt ccatgaaatg gattttggaa tggattttta tgcaccacaa
     9061 acaacaagag atcctaatgg tggacgggtg atgattgctt ggatgcagat gtgggatcgt
     9121 acattgccta cacatgagtt aggccatctt tgggcaggct ctatgagttt gccccgagag
     9181 ttaagagtga aaaaaaatcg attggtacaa aaacccatct caactgtata tagtcattta
     9241 gtttatcaac aaggcagtga aaatattgca gtgactgaca catcagtcgt atttcgaaac
     9301 gcgctgtctg attcttcata tttacatcta gtcgcagatt tggagaaagc tgaagttttt
     9361 acaattgaat tactgcggtc taagacggat tgtctcaaac tagcctatga cactgctaca
     9421 caggtttttg aattggatcg tcagaaacta ggaagagagc ttacaggggc ggaagaagac
     9481 cctctgaatg tacgaaaagt tgaagtacct ttgattgata atcagctcgt tcttgagatt
     9541 tttagagata cctcatcaat tgaagtcttt attaatgggt tagaagttat gacggctact
     9601 ttctatgagc tggaaaaagc tcgagatatc ttgtttcatt cgatcggaga aagtcgtatt
     9661 caagcttttg aaacagcgag tgtgaaataa atgtttgatt gcttaggcga tgaatcaatt
     9721 atttaatagg ttttgaagga taatgttttt agaaaagaac agatagctag atttcccatg
     9781 gatttgctat tagtattttg aaaaaattag taatttatga caaaattcat tatactaaac
     9841 ttgtgcagaa atgtgaggga tcaactatga aagcaacaat gaaagatgtc gcaaaattag
     9901 caggtgttgg agtaggaact gtttcccggg tattgaataa cggttctgtt aaagcttcta
     9961 ctagagaaaa agttgaagca gcaatgacag agttaaacta tcaaccagat ttttatgccc
    10021 gggggttaaa gatgaagcat acgaacacgg ttgctttgat tctacccacg gtatggcatc
    10081 cattttttgg cgaatttgcc taccatgtag aaacagcatt agaaagacaa aactacaagc
    10141 tttatttgtg taactccagt ggtaatagtg aaaaagaaca agaatatatc cagatggtca
    10201 gccaaaataa agtagacggc atcattggaa ttacttattc agatatcgat gaatacattt
    10261 cgagtaatct gccttttgtt agtattgacc gttatttcac tgaagaggtg atttatgtga
    10321 ccgctgacaa tgcggccggt ggacgtttgg ctgcaaatga actttatcgc cgaggatgtc
    10381 agaatgtagc atatatcggt gggtaccaag aaacaccgaa tgaaacgaag agtcgtcgaa
    10441 aatattttga aatcgagtgc cgtcaactgg ggatttccta taaagcgtta gatatgttag
    10501 aacctttaga ccatctctca gaacgtgtaa caaatttttt gttagagaat cccgatatcg
    10561 atgggatttt tacgatcaac gatccaatgg cattagctgt cattgatatg cttgaaaaaa
    10621 tagggaaaaa cgtaccagag gatgtccaag ttatcggatt tgatggtcaa aagatgacag
    10681 aaggagatcc ttatctagtc tcaacgattg cccaacctac aaagcaaatg gcggaagtgg
    10741 cagtggcaaa attaattgct gtcattaatg gagagacagt agaaaaacgt accgtattgc
    10801 cgattgaatt tggcgaaggt gacacaacga aacctttagt gagaaacgat tttgcttagt
    10861 gcaaatacgt ttcttttttt gaaaatgagt gaatgaaagg tgtagataaa atgttatatg
    10921 gtggaataga agccggtgga acaaagtttg tctgtgggat tggtaatgaa caattacaag
    10981 tgatagaacg gaaaagtttt cctaccacaa caccagaaga aacgatggag caagtgtttg
    11041 atttctttga taagtacaaa ggaaatatcg catcaattgg tgtaggagca tttgggccta
    11101 ttgatattca aatcgaatca agaacttacg gatatatcac caacacaccg aagttagctt
    11161 ggcaaaatta tcattttgta ggagctttgg aaaacgcctt gtcggttccc atctattgga
    11221 ctaccgatgt aaatgctgct tgttatggag aatatatggc agggtatgga aaaggtaaaa
    11281 atagtgttct gtattacacc gttggtacag gtgttggtgg tggtgctgta gtggatggga
    11341 aaatagtcgc aggttttagt catcctgaaa tgggacatat gctcttacaa cgtcataatg
    11401 aagatcaatt tgaaggaatc tgtccttttc atcatgattg tttggaaggg ttagcatcag
    11461 gacctgcgat tgaaaaacgc tttggaagaa aagcccagac tatctcattt gatcatcctt
    11521 attggacgat tgaggcagat tatcttgctc agtgtgtgta taacacaacg ttactatatg
    11581 caccggaagt aataatttta ggcgggggtg taatgaaaca gccgcaacta ctcgagttga
    11641 ttcgccaatc gtttaaagaa aaaatgggtt cctacgtgga tacgcctact ctcgaagaat
    11701 atctactaac tccaaggctt gctgacaatg cgggcttgat tggctgccta gctttagcaa
    11761 taaagaaact caatcattag aatagtattt attagaacta ttcataacat accggaagtt
    11821 taaaagggta ttctgaaact taaatagtct caattcattc ttctttcttg agaatctgag
    11881 tagagacttt tattctgttt caaattaata taattacg
//
LOCUS       DACCXO010000086        11792 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.087, whole genome shotgun sequence.
ACCESSION   DACCXO010000086 DACCXO010000000
VERSION     DACCXO010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.087"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..218)
                     /locus_tag="IVY70_002105"
     CDS             complement(<1..218)
                     /locus_tag="IVY70_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7959222.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLI"
     gene            complement(212..613)
                     /locus_tag="IVY70_002106"
     CDS             complement(212..613)
                     /locus_tag="IVY70_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7959223.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(800..2608)
                     /gene="uidA"
                     /locus_tag="IVY70_002107"
     CDS             complement(800..2608)
                     /gene="uidA"
                     /locus_tag="IVY70_002107"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP7959224.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(2631..4397)
                     /locus_tag="IVY70_002108"
     CDS             complement(2631..4397)
                     /locus_tag="IVY70_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP7959225.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(4410..5204)
                     /locus_tag="IVY70_002109"
     CDS             complement(4410..5204)
                     /locus_tag="IVY70_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP7959226.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(5214..6590)
                     /locus_tag="IVY70_002110"
     CDS             complement(5214..6590)
                     /locus_tag="IVY70_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7959227.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(6710..7357)
                     /gene="eda"
                     /locus_tag="IVY70_002111"
     CDS             complement(6710..7357)
                     /gene="eda"
                     /locus_tag="IVY70_002111"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP7959228.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(7441..8868)
                     /gene="uxaC"
                     /locus_tag="IVY70_002112"
     CDS             complement(7441..8868)
                     /gene="uxaC"
                     /locus_tag="IVY70_002112"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP7959229.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(8942..10021)
                     /gene="uxuA"
                     /locus_tag="IVY70_002113"
     CDS             complement(8942..10021)
                     /gene="uxuA"
                     /locus_tag="IVY70_002113"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP7959230.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(10024..11655)
                     /locus_tag="IVY70_002114"
     CDS             complement(10024..11655)
                     /locus_tag="IVY70_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP7959231.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
BASE COUNT         3739 a         2391 c         1518 g         4144 t
ORIGIN      
        1 tctattaatt taatttctag ctgacttatc atatattcat ctacataggt aacgaattga
       61 cctacctcgt taaatccatt tgtcaaacat aacttagcca tataagaatg ataatgtttg
      121 agtatcatct tgatcgaaat tggatctcct tctagagcag ataagataat ggatagtgga
      181 actaataggt actgccgctt tttagattta tctaccatag gtcatacccc attttcaatt
      241 gttcaactgc cttctttcgt tgataccaga taccacttgt gctcatattg agaattttac
      301 cgatttcagc atcattaaaa cctgcaaaat aataaagtag gataatcttt cttcttatct
      361 ccgataactg atcaattgct gctgataact tttgatctga aattaatatt tccattccaa
      421 ataagaaata gacatcttgt tcatcaaaat gagtttggga atacctgcct tcatttgaat
      481 ctgccaatgg ttcctcatta tcctttatgt tagcgtttcg ttttcgaata gacttagctt
      541 cgtttcgtaa tactttttta cagtaggcat caaattggtg ttgtatagat atgtcattgt
      601 aatgttgttg catgataata tgctcctttt tccccctttc cactaataca acaatcagaa
      661 agcccctttt tttcgaaaaa actagaaaaa aaacataaaa atatttatca ttctttaaac
      721 agaaaatctt tctataaaaa acaaaaaaaa acgattaggc taggctaatc gttttttgaa
      781 cattcttaaa aaatccaaat tattttttat aactgaaatt gggtattgat gaccatcgtt
      841 tctgtagata gtaagcaatc attttaggtt ttctatctct agtaaagatt cctttcttat
      901 ttccttgcac tcttaaaatc ccttgacttg ttgcaaaatc tgcaaaattc cacacttgtt
      961 caccaacaaa attttcacat gtatctatta cttcgtgatt tgcttcgtaa taagccactt
     1021 gatactcttc tgtaaacatt gttggtactg tatcatgaaa tccagctact gtatctgctc
     1081 catattctgt aaacattatt ggcgtatcag gacatctttc catccatcca tctagctctt
     1141 ttttcaacag atattttgca tgtgccagat cccctcccga aacataccaa ccataataac
     1201 gatttaaaca taaaacatcg acaatatctc ctaccttaca aacatctggc gttgatttta
     1261 gataagtaac gattgttaca ggtcttttct gcggatcaag ttctttagtt aattcaacta
     1321 aaggttcaaa atattcttta gcaccttcgg aatccgaatc tggttcattt gcaatagacc
     1381 acattacaac acttgggtgg ttcttgtcgc gttctatcaa ttccttgatc accgttttat
     1441 gagaattttc tgttcgtatc tctttccacg tattatgttt ttccacatta tcatctaata
     1501 aagtggccat gaaatttaaa tgtaacccta ctgcaggtac ttcatcaata actacaatac
     1561 cttctcgatc tgctaatcgc ataatttctt ctgagtaagg ataatgtgat gttctaaaag
     1621 aattagcacc tatccatttc attaaattaa aatctttaat atttacagcc tcattcaaac
     1681 cacgtccatt aacatacgaa tcctcatgct taccaaatcc tttaaaataa aaaggtttat
     1741 tattaatcaa aaatttgcca ttggtaacct gaacactgcg aataccaaat tcttctgtat
     1801 aggtatcgat cactttatta tcatcaatta aactaatttt catactatat aaataagcgt
     1861 ctagaggttc ccaaaaaatt ggattcttaa gctgaatcat tcctttcttt ccttcagctt
     1921 cagctacaag ttgagcctct tcgtcatata tcgctattaa gatttttccg gtccattctg
     1981 tatcgatatt gaattctaca cttgcatcca aatcagttat tcttgtatta atagtaatat
     2041 cacttatata gtctttcgga gttgtatata acttaacagg tctatgaata cctgcataat
     2101 tgaagaaatc aaaattaact aagttttcct tttttatatc cccattttcc tgtactgttt
     2161 ccttcataat ccccacaggt aaagtactat cgtcaataat attgtttaca gcaactgtaa
     2221 ttcgattatt cccgtctttt acaaaccgat taatttcaga ctcaaatggt gtaaacccac
     2281 ctttgtgttg agtaataaac tttccattca aaaaaacttt tgcttcatgt gttgcagatc
     2341 caaaacgaag aactaatctt tcttctaata atcttttagg aagagtcact tctttttcat
     2401 accacaccca tccaacatga tttctaatgt tctctgtttc aaataaatca ttataagaag
     2461 agggaactga aatcggataa ctctcttttt catttattgg ttctaaagca agttcttcct
     2521 gtaagtcatc atttgtatct aatttaaatc tccagagacc gcttaagtca attacttgcc
     2581 tcgtctctgt cataatggga tacaacatat aaaccactcc tatctaaata ttaaataaat
     2641 gtatccttta aaccacaata agcatctatt ggacttactc ccttaaattc agaaagaccc
     2701 attagtttgt ccaccaatat ttctaaagtt tcttgttttc catcataagt attaatataa
     2761 gtctttactt gaggtacatc agccaaatga aacggacact gtacagatac aaaaattgtt
     2821 ggaatttcat gaatgtaaaa cggtatatcc ggagttcctt tacttgctgg ccagacaatt
     2881 cgttgaactg tgcctggatt aacattagct atattaatga tcaagtcata ttttttagtt
     2941 aaatttttaa ttggtctctt agcagcatat attccagcta tatttttaat tttttcttct
     3001 tgcgataatc ctttcaattc ttcttcaggt gaaatgtatt tttcaacttc aaatcctttt
     3061 gctgacaaaa tctgtttcaa taaatcaatg gcttgaggtc tatttccagc aactacttca
     3121 ccaaacccac cttttacccc atgtacatct actagtaaaa tccttttata tttttctggt
     3181 acaataggcc aaatatcttg tttatcttta actaatgtaa ttgcttggtc agccacctct
     3241 ttaaaaattg atttattttc aattaaacca attttttcca gagcttcttg cttaggtaat
     3301 aaaatttgtt cttttgcttt tttgtgtaat cccaatttag cttttaaccc taaaattctt
     3361 tttaaggcat ctaataaccg atcatcagtg ataattcctt tacgatagcc ttccatcata
     3421 taattaaagt cttcatctgg atcattaaaa aatagaaata gatcacatcc agctgcaata
     3481 gaagtgggca ataattctga tcgtttcatt gccccagtca ttcctaacat atgactagca
     3541 tcagtaacta ctaatccatt aaatccgagt tttcctctaa gtaagtcagt gattaattct
     3601 tttgataaag ttgcaggaag taaatcttca tctttcagat caggattaaa atgttttgaa
     3661 taagctggca tatggatatg acctgccata attgaaggta atccagcttc tattaagcct
     3721 gagtatactt ttccaaaaga attatcccat tcttccgtac ctaaactatt aatactaaaa
     3781 gatagatgct gatctcgttc gtcaataccg tcaccaggga aatgtttagc tgcaggcaaa
     3841 attccactct cctgaatacc tttcatataa gctaaactaa gttctaaaac tttatcaggg
     3901 ttttgtccaa aagatcgact agaaatgata ggatttctcc aatttctatt aatatctaca
     3961 ataggagcaa aactccagtt acatccaata gccgatgctt caatcccaga aactctccct
     4021 aattcataag catatttcgc atcatttgtt gcaccaattt ttacttctac accaacttca
     4081 gtaccatctg tacaagctcc gttaccacct gcttccgtat tagcagcaat taatagagga
     4141 attttacttt ctgtttgtaa gatatagttt tgatcatata cctcatcagc agttcctgga
     4201 ttatatcgta cagctccaat atggtactga ttgaccatat tactcaagta atttttatca
     4261 cgactagatc ccatattaac aaacaattga cctatttttt cttccaaagt catagaatca
     4321 atcgtctctt caatccaaga aatagcatct tcatctaaat tatatggttt ttttcgtaaa
     4381 tctactttta tagtcatttt ctttctcctc tacttattag taagattttt ccaaaaagta
     4441 tctatatatt tttgttcaat atctacataa tctcccgata aacatttaaa taaaacatca
     4501 gttcttaagt tttcccatga ttctttttct ttattgacca aaataggata aaagaaaact
     4561 ttatttttat ttgcagcttc tagatcacca ggagaatcac caatcattaa tatcttatcc
     4621 aaatcacaac cattttcaat tacacttcga ataatatctt ctttttttcc tttatcttga
     4681 caacacagtt catcaactaa tgataataat tcatgtcttt cccattcttc ttgaacagct
     4741 tccttattag ctgagcttac aataattatc ttcccatatt ttttcagagc ttttatagta
     4801 tctctagcac ttaaaaaggg tttatctaat ccttttgctt gactaatctg ttgattgact
     4861 tcttccgacc attttaaagc ttttaataaa tcctctgact gagaatattc tatttccctc
     4921 ttcaaagatg gattagacaa ttcttttgtt agagataccc agtgctttaa tcgattaatc
     4981 gatgttacat catttttttc taatgtttta actaaaccta aaaatctgtt tattccacga
     5041 gtctctgaat ataaattaat attattccaa tcctgtagga atttttctct atgatgaaca
     5101 tcaaaaatct ctgcagcaat tggtccgaaa aagagattgt gtttataagt catcgtatcc
     5161 ataacacagc catctgaatc aatacaaaat atccaattat tcatatttct ctcctattta
     5221 tgtttttgtt ttaaaagctc gcaattttct aagactttat ctttagataa tggataaagg
     5281 aaaaataaaa ttaatcctac aatcagaaaa caaattgatg gaattaatgt tgaagcgtta
     5341 tataactttt ctaaaacatc tcccgtttgt acatttgcta aagagtcata gcctataata
     5401 gaaagagtcc aacctccgac gcctccagct agagcttgac ctatttttct agcaaaagaa
     5461 tataccgcat atacggtccc atcttctctt tgatcagact ttacttcttg atcatcaata
     5521 acatcagtaa tatttgccca aataacagta ttaaacacac ccaaacccat atagccaacc
     5581 gaactaagaa ttaagaatac atacatatta ctagctttta tcaaaaataa gatcaaatat
     5641 actattgctg aaaagaacat tcctactgat gccagttctt tttttccata ttttttactt
     5701 aaaaacaaag ttaaaggcga aacaactatt aaaaccaaaa taggattgat aaaattcatt
     5761 aaaactattc cctgtgcatc attatagtaa tcaggaaata cataattatt catactcata
     5821 attaataatt gagcaaggag taagaaaata gccgcaccaa ttatagaaag taatgcccta
     5881 tttttaacta acccttttaa agattttaac aatgaaactt tgtattgaga ttggtcaatt
     5941 cttactcgtt cagtagttag tttataacat attaaataac aaataagtgc acatatcgaa
     6001 aatattcccg caacaattgt aaaagttgat ccaccacgaa ctatttgagc gcctgaatca
     6061 tctcttgtat aaataaataa tggtgttaat acaccaatga ccaaacttgc taaagtagct
     6121 ccaacatttc ggaatgtcga tagcattgtt ctttcttctg taatagaagt aatagctgat
     6181 gccattgatc cataaggaat atttatagat gtgtaaaaaa tactccccca taatatatac
     6241 gtaacataca tgtatactac cttaacgctc atagaagctg aaatcataga agtttgatac
     6301 attaaaaaac ttgctaaagc gactggacca ctcattctta aaatccacgg tttaaactta
     6361 ccctgcttag ttgctttcga tctatcaaca atacgcccca tagttacatc agtaactgca
     6421 tcgataaatc ttgctactaa aaataatgtt ccaacggcag ctccgcttat ccccaatact
     6481 tttgtataaa agatcattaa aaaagaacta gcaaaaatga aagtaaaatc gtttccaaaa
     6541 tctccaaaca tatatcctat tttgtctctt ataccaaatt ttctttccat acaattctct
     6601 cccttctgtt gttaagcgtt ttcattctga tgaaagtacg tactatactc tgaagcgtaa
     6661 agagtcagta tttatttttt acatttcaga gtattatttg ttttttctcc taataattct
     6721 ttatctcata aaacttatct atatatttac tagctactcg acttacttct tcaaaatcac
     6781 ctttttcaac agaagccaat aaattcccac ctacaccaac tgcaacaact cctacttcaa
     6841 accaatcttc taagttctct atacttactc cgccagtagg cattatattt acgtgtggta
     6901 atggagcttg ataggctttt acaatggatg gaccaaaagc agaacctggg aatagtttaa
     6961 tgatatctac gccagcagta agtgcctcct ttatctcagt aattgtcata caacctggta
     7021 aataaggaat ttgatataaa ttacaaatct cagctgtttg tcgatcaaaa gatggactaa
     7081 cgatatattc tgccccagcc ataattgcga tacgagcagt aaacgcatct aaaaccgtac
     7141 cggctccgat tattacatct ttttgatcag catatttttt agcaatttct ttgattattt
     7201 cttcagcatt aggaactgta aaagttagtt caatcccttt aattccaccc gcaataattg
     7261 aatttattgc tttaatggcc tcatccttat ttttcccacg tacaaccgca atgactccag
     7321 ccgcttctaa cttacttaaa atttctactt ttttcatatc tatctccttc ctttatggat
     7381 acatactttg tctagctgta ctaacttaca aagaataaat ttaagctttt atttcaagta
     7441 ttattttcta cttccgattt caaaaaaatt aaaataatta tacgcattac gataacaaat
     7501 attctccact atctgactaa gatacatttc atcatctgga attcggcctg ctttaaccca
     7561 ctcgcttagt aattgacata atactcgacg aaaatactca tggcgaggat agcttagaaa
     7621 acttcgagaa tcagtcaaca tacctgtaaa attcgacaac aaactttgtt gtgccatcgt
     7681 agtcaactgt tgacgcattc cttcagctgt atcattgaac caccaagcac atcccaattg
     7741 gattttttga atcatacccc cttggaagct ttgcattgct gttgctagtg gaagccaatc
     7801 agttggattt aatgaatata aaattgtttt tggtaagcac ccactctttt cagcggtatc
     7861 gaacaatgct acaacatttt gtgtcaaact agcttgatcc cccatcgaat caccaccgac
     7921 atctactcca atttgttcct ttaatatact tgaattattt cttaaagcat ttaaatgcat
     7981 ttgcatcgtc caaccatatc ttgtatactc agacatcaaa tgtaattgaa tacctgtaac
     8041 aaattgtgca atttctgata aatggaaatc cgtttttcct gctaaagctt ttattaacag
     8101 attgtcgagt attttttcat ctgctatttc ataaaaataa gtattcaaac cgtgatctgc
     8161 taatcgacag ccaatttgat gaaaatgatc cactcgttgg ctcaatgctt gcttcaaccc
     8221 attaaagtct ttgattggta tagccgctgt ttgactaagt tttaaaatat agttcgaaaa
     8281 gcctgatgat ttgatactcc agaccttatc tggacggaaa gtaggtaata ccttgaaaga
     8341 attatcctgt ttagaaagct cagtatgata ccacaagtca tctgtaggat catccgttgt
     8401 acagatggct ttcacatcca tcatctccaa caattctttc gggcgatagg aatccgtttg
     8461 aattttttcg ttagcacgtc gccaaatttc tggtgcattc ttctcagaaa ttgtcaaatc
     8521 aatatcaaag taacgttgta attccaaatg agaccattca aaaataggat tacctggcgc
     8581 cttctccact gtttttacaa aagctaaaaa tttttcataa tcatcacctg gaccactgat
     8641 caactcttct tctataccat tagctcgcat caatcgccat ttataatgat ctccagctcc
     8701 tttatcattt aaccatacct gagtcaaatt cttgtattgc ttattttcta gcaaatatgc
     8761 tgggtctaaa tgacaatgat agtcgataat tggttggtgt tttgcaaatt tatgaaaaag
     8821 tcttttagct atttcattct ctaataaaaa gttcttatct aaaaacatat ttaaacactc
     8881 ctttccttcc gttagttttt agaagagaaa atttttcctc taaataaact cccttatata
     8941 attatttact cgcccccttt tcaagcgctt cccaaatacc atttaaataa actgcaccta
     9001 gagctcggtc atataatcca taaccaggtc taccggtttc tccccaaatc atccgaccat
     9061 gatctggacg aatatagcca ttaaatccat tatcgtacat tgtttttaat atcttataca
     9121 tatctagaga accatattct gatttatgcg gcgcttcgta aaaatcgagt ccattgtcac
     9181 ttaaatattt gatattacga acatgtgcaa aaggaatccg atcccgttga caaaattcag
     9241 atagaatttc atagacatca ttatctggtc gttctgcaat acttccagta cataatgtaa
     9301 taccattaga aggagaatcc actacattac acaaccaatc taaatcgtca cgatttttaa
     9361 caatacgagg taatccgaaa atggaatatg gtggatcatc tgggtgaact gccattttaa
     9421 tcccaacttc ttcacatgtc gggataattg ctttgagaaa atatgcaaag ttctctcgta
     9481 atttctcttc atcaacttct ttataggctt ctaataattg cttaatttgt gataaccgat
     9541 gaggttccca acccggcaat tcaaaattat tagtagaatt ttctacttct ctaataattt
     9601 cctccggatc agtagggata ttttttgaaa tataggctaa cgtagaagac ccatcatcta
     9661 aacgataatc caaatccgat tttacccaat caaatactgg cataaaatta taacagataa
     9721 cctcaatccc aaattctgcc aaattgcgaa tcgtctgttt ataattctca atgtattgat
     9781 ctcgtgttgg taaaccaatt ttgatatcat catgaacatt aacactctca ataactttca
     9841 aggtaagtcc attggcttcg acttcatctt ttaaagattt aattcgttct ctcggccaaa
     9901 cctctccggc tggtatatcc attaatgttc caacaattcc tttcattgct gggatctgac
     9961 gaatttcttg tagtgagatt ttttcttcat ttgccccata ccatctaaaa gtcatttcca
    10021 tagttacttt cctccaactt ctgttatata tttattgatt gttttacgaa ctgcttgagg
    10081 acctactagc atttcgcaaa agattttttc agttttcttt cctaccccaa cttgatataa
    10141 atctaaacca aagatttctt tattttctaa aatttcctgt accgcacaat gaatctttga
    10201 aggatcttgt tcacctaatt tcaatactga taatttatcc tgcaactcta ccaataacgg
    10261 atcaggactt ggcacaaaat tttccccttg atcatcgact gccagtaaat aacgtaacca
    10321 agcagcaata gcgataggaa taaatgttag attttctgca tttccgtctt tttcgacata
    10381 ctttttaatt gtttcaccat agcgaatcgc tactttttga gaagtatctg ttgcaattcg
    10441 ttgtggtgta tctggaatat agggattagg caaacgttta ttaattactt catcaataaa
    10501 ttgttttgga tcaataatgc ctggattttc aactactggt aaaccttcta aataacctat
    10561 tcctttgatt aaaccaacca aatcatcatc ctgcatctct gctgaaattg attgatatcc
    10621 tagcagacaa cctgttactg ccaaagcagt atgaagtgga ttcaagcttg tcgtaacctt
    10681 catgatgtct gctttatcaa ccgtctctct atctgtcata atcacaccag ccttttccaa
    10741 tttaggccgg ccatttggaa aactatcttc aataactaaa taatgaacta cctcagtatt
    10801 agtgaaagca gcaatattgg tatgtttttt tgtgtgaatg atatctaaat cctcgagccc
    10861 catcatcaat agatcttcct ttacattctc tgaaggattt ggcgtaattc gatcaatcat
    10921 actccaagga aaagtcactt tttctttatc ctttaaataa gcaataaatt tttcagaaac
    10981 atatccttct ttttgccaag cttctgcaat cgtaaaaata ctatcttgaa aacgttgacc
    11041 attctgtgag aaattatcag tagaaaccat cgcaatcggc aactctccag cattaaatct
    11101 gctatataac aaggatgtta caatactcat cgtatgttga ggattgctag gtccagattt
    11161 gatatcagct ttgactaccg aaaagaattg ccctttagaa tcttttaaac tgtaaccttt
    11221 ttcagtaata gtaaacgttg taaactgtaa tttaggatct tcaaaatatt taaccactct
    11281 ttgatagtcc gctatattct tcggattgca atataaacta tcagccgtta ctgccaaaag
    11341 tctttttttg agttcaccat ctccactcat aacaatttcc aaaatgtcat tgttatagtc
    11401 atgataagct ttttccacta cttcttcatc aaatgtttca cacacaacta ctcctgtttc
    11461 taattcattt tgatctgcta agcattgagc aatttctgca tgaaatcctc gataaagatt
    11521 tcctccacca aaatgaagcc atactggatg ttgctggcct tcttgcttaa cttctttgac
    11581 atcaaaacac ggcagtagaa tctgcatttc attgaattct tcgatagaat tttgccaatt
    11641 tttaatcgaa accataatta acctcctcaa actgatatgt tagttttgat acattttata
    11701 ttttaaaacc cattgtaacc gttgtcaata taaaaaggga ataaacttgc ttttttctac
    11761 catatcagaa tgattgtatt ttttattcag aa
//
LOCUS       DACCXO010000087        11771 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.088, whole genome shotgun sequence.
ACCESSION   DACCXO010000087 DACCXO010000000
VERSION     DACCXO010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.088"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(167..544)
                     /locus_tag="IVY70_002115"
     CDS             complement(167..544)
                     /locus_tag="IVY70_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959232.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(844..1725)
                     /gene="galU"
                     /locus_tag="IVY70_002116"
     CDS             complement(844..1725)
                     /gene="galU"
                     /locus_tag="IVY70_002116"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP7959233.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1768..2787)
                     /locus_tag="IVY70_002117"
     CDS             complement(1768..2787)
                     /locus_tag="IVY70_002117"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP7959234.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(3420..4424)
                     /locus_tag="IVY70_002118"
     CDS             complement(3420..4424)
                     /locus_tag="IVY70_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7959235.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(4437..5486)
                     /locus_tag="IVY70_002119"
     CDS             complement(4437..5486)
                     /locus_tag="IVY70_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7959236.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(5503..6324)
                     /locus_tag="IVY70_002120"
     CDS             complement(5503..6324)
                     /locus_tag="IVY70_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7959237.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(6311..7090)
                     /locus_tag="IVY70_002121"
     CDS             complement(6311..7090)
                     /locus_tag="IVY70_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7959238.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(7107..7577)
                     /locus_tag="IVY70_002122"
     CDS             complement(7107..7577)
                     /locus_tag="IVY70_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7959239.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(7589..8008)
                     /locus_tag="IVY70_002123"
     CDS             complement(7589..8008)
                     /locus_tag="IVY70_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP7959240.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(8089..10857)
                     /locus_tag="IVY70_002124"
     CDS             complement(8089..10857)
                     /locus_tag="IVY70_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7959241.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            11057..11281
                     /locus_tag="IVY70_002125"
     CDS             11057..11281
                     /locus_tag="IVY70_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959242.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3416 a         2480 c         1905 g         3970 t
ORIGIN      
        1 ataaataaca taaaaaaagg aatggcttat cccgatacaa caagcattcc tttaaaaaag
       61 attcttttat ataagtgaaa aaagcttttt tccaccaatt aaataaatct ctcatctcaa
      121 tagtttatcc attattttat ctttttttgc gtatacctac taaatattac cgtcttcttc
      181 tcgttcttct actttgttcg agagaatctg tactagaaaa agtagttgta tgtctacctt
      241 ttctgcctat ccgagacttt cttttcatat atgggatata aatgattaga tacagtacag
      301 caaaaaataa agcaaatacg caagcttccc aactacctaa cgccaattca accatcaagg
      361 tagcaacccc aacggatgct gcataccaga taaaaatcag aaacgtttct ttttttctga
      421 ccatgcgaaa catgataaat agactaataa gcaatagtgg caacacaaat atccaattgc
      481 ttccttccat cagccacttg agtatatcaa gcagcgactg tgcaacagcg actaattttc
      541 ccatagtgaa cacacacctt tttttataat taaaaaattc gcctttttat tctactatga
      601 atcttctggc ttggctttca tttctcttat gtaatcattt tcttttatac aacttaaaaa
      661 aaaattaaac cattagcata atattttctg aatctattca cgaaagtctt tttctatctt
      721 tcgttttcca cgactcaact gatctagagt caaaaaacta aaacgacagt tgcgcaaatg
      781 acgcacaagc tgccgcttta ataacaaata gcctttcatt ctcagcaggc cacttatttc
      841 cagttaactt tctgttctta gctttttact caactggata atatatgcac ttaatgaagt
      901 cccaatttcc gaatgttgaa gtccatattc aatatttgtc ttcatatatc ctaacttatc
      961 gcctacgtcg tatctttttc ctttaaactc tctcgcatag accgcctctg tctggttaag
     1021 agtctgaagt gcatccgtta gctgtatctc gtttcctgct cctggttctt gagtttctaa
     1081 tatctcaaat attttaggag tcagtagata acgtccgata atggctaaat tacttggtgc
     1141 ttttcccatt tcaggttttt ctacaaaccc ttttactctg caacgaccct tttcgctatc
     1201 tgttttagga tcaataattc cgtatttaga tgtttcattt tctggtacac gcatgacggc
     1261 aacagcggaa gcttgtgttt cttcataatc catcatcaat tgttgagtca atgggatatc
     1321 atctttcatc aaatcatccc ccagcatgac gacaaacggt tcatttccta taaatgcttt
     1381 tgcttgaagc acggcatccc ccagtcctct tggatgagat tgacggatga agtgaagatt
     1441 gacttgagtc gtttcttcaa ctaaagcgag taattctgtt ttttcctttt cccgcaaatt
     1501 gttttccaat tcgatatttg catcaaagtg atcttcaatg ggtctttttt cttttcccgt
     1561 tacgatcaag atgtcttcga ttcccgaatc caatgcttct tccacaataa actggatcgt
     1621 tggtttatcc acgattggaa gcatttcttt tgccatggct tttgttgccg gcaaaaaacg
     1681 tgttcctagc ccagcggcag gaatgactgc ttttttgacc ttcacttgct gttcctccta
     1741 aacgacgatt aactattgat catctattta acgaagagaa aattcattct ccatttttcc
     1801 gtcacgtaac atgatttctt ttgctgcttt tttgatatct tctccattat acaaaacatt
     1861 atagattgtt tgagtaattg gcatctcaac acctagctgt tgagctagct ctactgctgc
     1921 ttttgtcgtt gccacaccct caacgaccat tcccatgttt tctagaactt cttccagctt
     1981 atgtccctgt cctaacaagt taccggcacg ccaattgcga gaatgaacac ttgtacaagt
     2041 aacgatcaag tcccccacac cactcaaacc gataaatgtc agaggattgg cacccatcgc
     2101 gacaccaaga cggctgattt ctgccaatcc tcgagtcatg attgctgctt ttgcatcatc
     2161 tccgaatcct aatccatgga tagcgccggc accaattgca atgatatttt tcagtgctgc
     2221 accagtttct acgccaatca catcattatt tgtataaatc ctgaaataat cattcatgaa
     2281 cagtttttgt acataggctg ctgctttttg atcaatactt gcagctgtaa ttgttgtgat
     2341 atcatgtact gccacttcct cagcatggct aggaccagat aatacaacaa ttgcctgtct
     2401 tttttcttct ggaatttctt cagccaaaac ttctgagatt cgtttatgcg tatcttgttc
     2461 tagtccttta cttgcatgta taatgattgg cttagtattc attttaccaa taagttcctg
     2521 tgcgactgaa cgaatcgctt ttgttggaac aacaaataaa atagcatctg cgttatccac
     2581 tgcttctgcc aattctttat atccgataat cgattctggg atcgtcaaat caggtaaaaa
     2641 atgctgattt gtatgatact tattgatttc atcaatctgt tcaggtacat ttccccaaat
     2701 acggacatca tgcccgtttt ctgccaatgt ttgtgctaaa gcagtgcccc aagatccagg
     2761 accaagaact gcgatttttt gtttcatgga tattcctcca aataaataat tgataatttc
     2821 cattatagta tacatttctt aaagttggga ctaatttcca ttgtttttaa gtgtttgttt
     2881 acttctttta tcgtttttac ttgaaataat caatgatatg ttacaatagt ctacatttta
     2941 tttttacact cgttatgctt tgcttgttcc taagaggaag cagttttgac aatgttacta
     3001 ccagcattac tattaggagg ttaggaaact tctcctagtg agtattgatt taaaatacat
     3061 aagagacaga ttgaacattt ctaaatctgt caggtcctgc aacggacaga ttgtctctgt
     3121 tgacggttga cataggaact tttgttttag tcaacattct ggatacaaaa cgctatgtaa
     3181 actggcgcaa ctcgttgtac ttggactttt actacggatc catgcaacaa ctaaatagta
     3241 ctattgcata acaaaaaatg gttggcaccc acatgtccca ttaaacagat tataagttga
     3301 actgtagata atgcaaaaaa tgtgttttat aacgtaattg atgccaatat tttaaaaaaa
     3361 taaagggagc agttatcgct gctctcttta ttttttatat tctttttatc cagtctatcc
     3421 taataatcaa actgacgata gtagcggcgt atctctaatg gatggcagtt caactgctcc
     3481 acatgagcat cgatgcgttg tgtcactaca tgcatcaata agaaagacaa aacacgtccg
     3541 cgatattttt cactgatacc ttctactgga tagtcttttg tgtctatcaa tgtataattg
     3601 ctgcaaattt ttggcaaaag tttagccaca cgttcagata acactctctg ttcatcttcg
     3661 cctaaaaata atgtgactgg ggttgtttct tctacgatct ctaacgtccc gtgaagaaac
     3721 tcggcagcat ggatagattt cgatggaagc cagctttgtt cttcccagta acacatggca
     3781 tatgaataaa cagctcccca ttgattccct gctccaataa aataatgcat actatcacgt
     3841 cgatgttttt ctgcaaatga taagccaaat gcatctgctt ttttctctgc ttccactaac
     3901 cctgttggta aataacgttc gagctgttca tagtattctg aataagcttc gaattctcca
     3961 ttcaaataca tcaatcgatc tgctgccata aaaaatttga tttgttctgt tccaggagct
     4021 ggataagtga ccacaaaatc gcatagctga gacaacttac tgttcgcttt atcgataaat
     4081 ccaatcagtg tcgctcctac tttcttgatt tgttctactg ctttcactac ttcttgagtt
     4141 gtaccggtaa cagaggacaa aataacgata gaatcttttg ttaatctttt gtttccagta
     4201 gtgtaatatt ctgcagcatg ctgtacaaag acaggcaagt tgcttttccc attcatataa
     4261 gtgacagctt gcatcgagga agcatacgtt ccaccgattc ctagataata gacagcgtca
     4321 aatttttccg cataaagttt atcgatgatt tcttctactt gcggacgtaa agctagtgcg
     4381 ccttttattg ctttgatttg ttcttcttca ttgaatttca gcatgatttc cacctcttat
     4441 aatttacttt tcaagactgt atcaaagtct tcaaaaatct ttttgttcaa gtcaatacct
     4501 ttagctactg cggttttata agcgaatagt tgaaacggaa cgacaagcgc taatacagac
     4561 agatattcat cacattctat tgctaatcca agtgttttct cgtttcttgc tttttttgtg
     4621 gtaatggtga gagtctctcc aacgtaccga ctcatatatc tttgcaactt ttgcgaacgt
     4681 tccttattta cactttcccc ttcaataaag aatagcaaat gcgaagcatc tgcctccagg
     4741 tatgggccgt gcatataagc ttctaactca aatccttgtg acggaacgcg gattgtttca
     4801 gtcaatttcg tctctgcttc tttagctgtt ccccagtttg gaccatatcc aatggtgacg
     4861 aaacgctgtg ctaaacgaaa taaactttga tatccatcaa aaaattcttc cgttttttga
     4921 ataatcgctg gtaaatggtt gattattttg cgcaactgtt gcatgtagtc ttgttctatt
     4981 tttttgctga ttttattttt acttattcca attcctatcc ctaagagtag taactgtaaa
     5041 acagtcgcag aatagccttt tgtaacgaag cctactgatt ctaccccggc acctaaatca
     5101 ataatttgtg cagcttctaa agcaagtgga ctttgcacgt catttgtgat agcaacaaca
     5161 ggtaaccctt gtttttgtat catttttact gcttcaacag tagaagcact tttcccgctt
     5221 tgtgacacag caatcacagt atcgattgaa ggagaaagtt ttccataatt gaaaaaataa
     5281 aaaggttctt gaatatcgac agtaagatca ccataagatt ccaatgctgg cttcgctgct
     5341 aaacaagcgt tataagatga acccgttgct aagatcagta aattcactgt ttttttcata
     5401 tgttctcttg tcgaaaaatc attctttttc aagatcgtac ttagtacttc cttttcttca
     5461 tagatataat cttgcatcgt aaacattttc tctgctcctt ttttaaaaaa tccctatcca
     5521 cgaaccaaaa attccaaaga aagctagtcc gatcaagatc gttaatggtt tcacttcttt
     5581 cttcaataga taaaacacta tgccaaatgc tccaagacat aagatatttg gaacgatatc
     5641 atcaaaaata ctttgaacag tcacagcatt ttctcctgaa ccgattttta aaggaatatt
     5701 gatggtgatc atcgtggcag tcattgctcc aacgaccatc agtccaataa tagacgcgcc
     5761 taatgtcaaa ctttccatca taccgtttgc ctggatcttt ttcaaaaaac ccgttcctaa
     5821 gcgatatccc caacctgttg ctagatagcg aaacagaatg tgcggaatat tgaaaatcaa
     5881 aatgaacaag atagggccta aaatatttcc ttgcatggct aaagaggtac ccacacctgt
     5941 ggcaatcaat cgcaaagttc cccaaaagaa agaatctcct aaccctgcaa gaggtcccat
     6001 caaagatgtt ttgatactat cgatcgcagt cacatcaaat tcaggatcat ccttattttc
     6061 ttcctccatt gcagccgtga ttcctaaaat cagtgtaacg atatgtgggg tcgtgttaaa
     6121 aaactctaga tgccgtttca aagctgctgc catttcttct ttcgactgat ataacttttt
     6181 caaaatcgga atcattgcat aaacataagc catattcatc tgacgttcat aattccaaga
     6241 aaactccatt tgaaaagaac gccaaaatac gtgatttagc tcttttttcg ttattttgct
     6301 gtttttgatc tcagaattct tcatcttctt ctacctcccc tgcagttgtt tgaagtggta
     6361 gttgacggac attcataata ttgactacca caactgctaa aatcgcacca aaaatagcta
     6421 ctccggtcac aggaatctct aagtaagaag ctatagcaaa acctaagaaa aaatatggtg
     6481 caacttgttt gttgatcagc aaacgagcaa gcatcgcaaa accaagagct ggaatcaacc
     6541 ctgtagctac tgacaacccc ttttgaacga agtcaggaat catatttaat agatttccga
     6601 tcgtattgct tcccaccata taagaaacca ttaccacaac accaagcatc aaggaaagac
     6661 cgataccggc aagcaagtgc atatgctcga ctcctttata atttccttct tctgcataat
     6721 tgtctgcttt atggttcata attggaatca aaagccctaa gtaaatattt ttcaatatca
     6781 atgtcaacgt agcaatcggc aaacctagta aaagcgcggt ttctgtaccg gctccagctg
     6841 taatagcaaa agccgtccct aaaatcccac cggtcacaac atcaggtgga atcgaagcac
     6901 ctactgagaa cgatccgata aatgctaact ctaaagttgc gcccataatg atcccagttt
     6961 ttacatcacc taacacgatt cctgtaaaca gccccgtcac aatcggacga gaaagcaacg
     7021 atgtccctag tgcatattct gattgtgcga caaatgcaac aattcctaat aagactgctt
     7081 gtacaatcat tgaggttctt cctttcttac aaaacttctc ttacatccac ttttttatct
     7141 gctggtactt gacggatttc tacttctatt tgtttgtcaa ttaatcgttt caagcgttcc
     7201 tcctcttctg gtaaaatatt taccgctttt ccgatgctgc gagtaccagg ttttgccttg
     7261 atccctccta agttgatggt tttgatttct ggatacgctt ctgccagctt atatgcatcc
     7321 tcgatcgatt ccactacgat gaataactta tatttatctg tcaccccgct tttcaacgcg
     7381 gcaatcgagt catcaattga tttgatcact agtttgactc cttgtggctt ggctaatttg
     7441 atcgttgtct ttcgtatctc attcgtaggt acatcatcat tagcaatcaa aatacagtct
     7501 gcgcctattg tttgtgtcca tgaaaaagct acttgtccgt gtaataaccg atggtcgact
     7561 cttgtcaata caatcatttt tcttcctcct aaaattcgtc ttcttctatt ttctgattga
     7621 gccgctcgtt acaaaactgg atcatttttt ttgaatcagt tagcacctgc tggatcaaaa
     7681 cagttgtctc tacttcagcg tccaaccgag atactagctc aatcaatagt ggcaaattca
     7741 atccggcaac gagatagaca tgatcccgat tgatatattg taagaattcg ttgttgacac
     7801 ttccgccaaa aagatccgtc agaacaatga tttctttatc tttgtacctc ataaaaagct
     7861 gccttactgt ttccgctaaa tcaaaattct catcagtata gcaatcgaat acagtaatag
     7921 aatgattttt cccatagatc agctctaatg aatctaatat ccctgaagca aattttccat
     7981 gggaagctaa gataatttct ctatccactt tttcatctcc tcttttcact tacacatact
     8041 aaaaaagcaa gttgcatgcc aaaattagtg gcaacaactt gcttatgttc aaaattcttc
     8101 ttctatcgag agaaactctg tatcttcact caaaatatca tagatatagg caatctcaga
     8161 attggggatc atgacactat aatgctcttc aatgacacta aacgcagatt taatagcaga
     8221 taacccttct ttttgacact gcttccattt ctctaatcct tgataggttt cgattggcat
     8281 ttgtcggatc aatcgttcaa tgagacagct cacatggaca tacaacgcta attttttggg
     8341 attgctgatg cttcgattca actgcacctc taattctctc ataaaatgat cgatttctcc
     8401 catcaccttc tctgtatcta gaatagtcac ggaatccagt actttttcca atgaaaaatt
     8461 acgaatgatg cgctgattga atatttcctt ctctgaagca ttcatagtag tgtccaacca
     8521 ttcagctagt acagacatct tttcccccga gatgaggtct tctaatgaaa gatagtcgat
     8581 attggtgata tcaggatctg tggtgccaat cattcctacg acttcgtata tggaaaataa
     8641 tgggtcactg tttttcttat cttttaattg ctgatattca tatgggatga ttttcaactg
     8701 acaagccgta ggtaagcttt tttctagcag ttcacttaag tggaccgccg taccgattcc
     8761 agtgaagcaa gtagtaatga gtgctttggg tttgttttcc tctggataga gcagtcgcca
     8821 ttctggctgg atctgtcgaa cagtctcttc tgctatctct tctaatgata catttttttg
     8881 caggcattcc cctactgtga ttgctaaagg tgtcgtcaca ttattcaaaa tcaaaagcgg
     8941 tacggataac tgttttttaa aaaactgatg gatttctttc agagatccca tgtctactaa
     9001 tatgatcaat ccatttgaaa catcgctgtt ttcgcaataa tcaaggattt ctgaagcaat
     9061 ctgctgtggt gtgacatcta gtggcatatc aaatgattca aatagatcct cttgtaataa
     9121 acgatttact acatttgcca tactgctagc agttgcataa ccgtgagcag caatcatcgc
     9181 ttttggcagt cgtttttctt ttgaccattt cgcttttttt agatagatcg tcagtaaaat
     9241 ccgatccata cttgtgtctt caatatctaa tttgatctta cttaattcca gaattcgttg
     9301 gacatagtga taactagatg gataagactg agaaacttgt tgttctaatt ctttgattag
     9361 ttggataata tcatgttctt ccggtaacca tttgatagtg cttcgctgat agagatagta
     9421 actcaaggca tagacactgt ttccatcgaa tttgatttga taggcagttt ccatttgtcg
     9481 aaaggtctct cggatagatt gtgtccaata aaccagcagc tcgtgttttt cttgtctact
     9541 ggtctcaaac agcaaaaaat caaataattg aatcacttct tgctttaatt ttccttcgca
     9601 agaatatagc agacagttat ttttcttgaa atcaattagg atttgctcaa aacttttaac
     9661 gattcgctgc tgttcgggct gttgcttctc cattaattgc tctaaggtct ggtcttgcgt
     9721 aatatagatt ccttgctgtg ctgtattttt agtaataggc tgcgcaagta gttctttttg
     9781 caaatgatgg atcgtgacat ggatttcagg ctgttcacgc tgttcagcat atgcttttgc
     9841 tgctgaaact ttgacgatat ttttcagctc tcctatgttt ccggataatt tctgacttgt
     9901 taaaagtgac aaagctggtc cactgactga caaagggctc cccatttttc tttgttcttc
     9961 gagaaaaaaa gagtaaatca gttctagccg ttcattacga ctacgctgat taagtgcagg
    10021 tagttcgatt cggataggta ttcttcgaat gaaagttgtt aagaaatgac tagtaatctc
    10081 ttcagtcgta gcaaaaaaga gacgtgtttt gatccggcgt gacaaattag gttctcccat
    10141 acgataaatc acaccttgat caagataggt gaacagtttt tcctgacctt ctggtgttaa
    10201 gcgatgtacc tcgtctaaaa acaagatccc accatctgct gcttcaaaag cccctttttt
    10261 attttcttct gcaccagtgt acgctccctt aacatgtcca aacaaattgc tagtcaaaag
    10321 ttcaggatta tctgcatatt gtgcacaatt aaaagtgatg aatggtgcag aatcttctag
    10381 cagatcatga agcagacaat actgatagac caaattaact aaataacttt tacctgtgcc
    10441 actttcgcct gtaatcaaaa gtggtaatcc gccatctgga tagtaaagag ctgttttgat
    10501 ttgttcgata ctttctttca aactggcgtc gtatccgatc ataaaagaga ataagtcttt
    10561 tggttgcgcg aaaaaaggct gttcctcttt gagatcatcg atagaaaaat aaaaattcgt
    10621 gcgtaactta aaaaattgtt tttcaaaagc ttctttgtgg aaataaatca ctggtcgaga
    10681 attgattttg actagtagtt gctcatcatt taactggttc aaataatgac tgactgtgtt
    10741 tctttttacc ccaaaatgat cagctaacct ttgtgctgta aagacctcgt tcatatgttc
    10801 taaatcgaaa aaggctgtct ggttctctaa gtaacataaa atttccttct tcaacaactt
    10861 attcacccct cctccatcat agaaaacgga ttcattttgt caataaaatc tttaataaag
    10921 aattgaaaaa tatatcttat tatagacagc agcctactta tcccttataa tttttattct
    10981 aaaaaacgta cagttccttc cagtttttat actggaattt caacatatga aagaataagg
    11041 aggctatgac tattcaatga aatcggataa atataaaaac aggaaacata cttcctggaa
    11101 aacttttcga gaaatcatcg gatcttatct cgctgtagtt gtatttttgc ctttgcttta
    11161 tatggctatg acagaacaaa ctcgttttat tggcgtagat acgcaatata tagccgaaac
    11221 aggattttta ttcatcttta tcttgatttt tgcgttctat aaatccttcc atacaaaata
    11281 agattcacag atttcaactt tcctgtctgt ttttagctat tacaaatcct tatatgtggt
    11341 ataacaacaa tttattggca taatgaggaa aaacgagtta tgatgctttc agagagtaaa
    11401 gaggtactgt tgtcaccatc ctttttattg attacgtaca ccactcttag ttttttccca
    11461 ttgattacga aaacaaggta gaaacattga tgttcctacc ttgttttctt tttttatttt
    11521 cagatcaaaa tctcaagtag cttttgactt gatgtcggga ggttgtcttc gttccatcaa
    11581 acctgataga atctgcgcca aaccatagtt ttctccacta ttatttattc ctctaagata
    11641 gtacccacaa taaacgaata aatatttttc tcattttcat actccttctt tgaaaatcaa
    11701 ttagatttct ctcaacccac ttttttacaa aaattacgta gcaaaaaaga ttgcaagaac
    11761 aaattaaaaa a
//
LOCUS       DACCXO010000088        11635 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.089, whole genome shotgun sequence.
ACCESSION   DACCXO010000088 DACCXO010000000
VERSION     DACCXO010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.089"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IVY70_002126"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IVY70_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP7959243.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IVY70_002127"
     CDS             complement(322..891)
                     /locus_tag="IVY70_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP7959244.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IVY70_002128"
     CDS             1077..1979
                     /locus_tag="IVY70_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7959245.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IVY70_002129"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IVY70_002129"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP7959246.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IVY70_002130"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IVY70_002130"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP7959247.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IVY70_002131"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IVY70_002131"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP7959248.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IVY70_002132"
     CDS             8547..8732
                     /locus_tag="IVY70_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP7959249.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IVY70_002133"
     CDS             complement(8794..9990)
                     /locus_tag="IVY70_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7959250.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IVY70_002134"
     CDS             complement(10160..11596)
                     /locus_tag="IVY70_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP7959251.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
BASE COUNT         3428 a         2484 c         2044 g         3679 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaac
//
LOCUS       DACCXO010000089        11634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.090, whole genome shotgun sequence.
ACCESSION   DACCXO010000089 DACCXO010000000
VERSION     DACCXO010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.090"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(183..2258)
                     /locus_tag="IVY70_002135"
     CDS             complement(183..2258)
                     /locus_tag="IVY70_002135"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP7959252.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRVSRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQITAMALSL
                     ASLDQIITK"
     gene            complement(2260..3177)
                     /gene="glyQ"
                     /locus_tag="IVY70_002136"
     CDS             complement(2260..3177)
                     /gene="glyQ"
                     /locus_tag="IVY70_002136"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP7959253.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3561..4373)
                     /gene="recO"
                     /locus_tag="IVY70_002137"
     CDS             complement(3561..4373)
                     /gene="recO"
                     /locus_tag="IVY70_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP7959254.1"
                     /translation="MSQLVESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4516..5415)
                     /locus_tag="IVY70_002138"
     CDS             complement(4516..5415)
                     /locus_tag="IVY70_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP7959255.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5430..5831)
                     /locus_tag="IVY70_002139"
     CDS             complement(5430..5831)
                     /locus_tag="IVY70_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP7959256.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5809..6285)
                     /gene="ybeY"
                     /locus_tag="IVY70_002140"
     CDS             complement(5809..6285)
                     /gene="ybeY"
                     /locus_tag="IVY70_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP7959257.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6302..8494)
                     /locus_tag="IVY70_002141"
     CDS             complement(6302..8494)
                     /locus_tag="IVY70_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP7959258.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEVQEE"
     gene            complement(8518..9489)
                     /locus_tag="IVY70_002142"
     CDS             complement(8518..9489)
                     /locus_tag="IVY70_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP7959259.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10195..11574
                     /gene="brnQ"
                     /locus_tag="IVY70_002143"
     CDS             10195..11574
                     /gene="brnQ"
                     /locus_tag="IVY70_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP7959260.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3164 a         2430 c         2128 g         3912 t
ORIGIN      
        1 gttttcttga ctagggaaat tcctaattta aaagtaaata aaacagtaac aagattatta
       61 ttaatctttt tcatttttat ttcctcactt ttcatgagcg ttttgaaaat tctagcaaag
      121 aataaaataa caaaaattat taaaatagaa ttaataacag cttttatatt tataacaaaa
      181 gactattttg tgatgatttg atccaagctt gctaatgaca aagccatggc agttatttga
      241 ctaagttgtg ttagtcggtt cttacgtact gctggttcat cagccatgac catcgtttga
      301 tcaaaataat tttcgatcag tggacgaaga gcgacaagct ggtcgtagtt ttcgtggatc
      361 gtttggtcag caaaacttgc tttcattgct tctactgctt tgtgtaattc tttctctgcg
      421 tcgttttcga acagctcagg gtctacttct gattgttttt caagctgcgc ttttttcgct
      481 aagttcacga cacgtgtcaa tgcttccatt gaaggtttga agtcttcatc tgcaagatgt
      541 tgtttgaaca attgtgctgc tgcaaatact tttgtcagat ctttttgttc tgcattcaaa
      601 acagcttcga tgacatcatg acggatattc ttcgttgtca ataattggcg cagacggcct
      661 ttgataaagt cgatcacctg ttgttgtccc gctgacaagc caatgccaaa acgatccgta
      721 tctttactga taacttcact aatcgtttct tgcagaacag acaaaggaaa ggcccatttt
      781 ttcgattcga tgattcgaac gacaccatat gcttgacggc gcaaagcata tggatcgttt
      841 gagccacttg ggatcatccc aactgcaaag aaagaaaaga tactatctaa tttatcagca
      901 atcgctaaaa cagaacctac tgtagtctca ggtaaatctc cttcactgga tataggcatg
      961 taatgttcac ggatcgctgt tgctacggct ggtttctcgc ctttaagcag agcatatttt
     1021 tctcccatga tcccttgtag ttcagggaat tctcctacca tgttggttac tagatcaaat
     1081 ttatagattt cagcagcgcg ttgcagatcc atcaattctt cctcagatag tcctacttgt
     1141 tcgcctatga tgcgagcgat gacttgtacc cgttgcattt tctcatagat gctaccgatt
     1201 ttttcatgga aagtaacagt ttttaatttt tcaacgtatt ggtcgattga agctttcttg
     1261 tcttcattat aaaagaattc cgcatcttct aaacgagcag tcaatacttt ttgattgcct
     1321 cgtaccacat tttccaattt tacccgatcg ccattacgag cagaaataaa atgaggaagc
     1381 aacatacctt gatcgttccg tacttcgaaa taacgttgat gttctttcat agaagtgact
     1441 agtacttctt caggaacaga tagatatttt tcttcgaagt cgccagaaaa gacagttgga
     1501 tattcgacta gattgttgac ttcttctagt aaatcttcat ctagatcaat gatccagtga
     1561 tttttttctg ctaattcgtt tgcttgtgtg atgatcattt cttttctttt ttgcggtgaa
     1621 gcaataacaa attgttcatt caatttttct tcgtattctt ttgcatgcgt aaatgtcaca
     1681 tcttcgccta agaaacgatg acctcttgaa actctgtctg ttttgacatc caagatctca
     1741 aacgggatta tttcgtcatc taacaatgcc acgatccagt gaatagggcg aatatattca
     1801 aaatcgtatt ctgcccagtg catcgttact gggaaagtga ggcttttaat gacatctgct
     1861 aaacctgtca atacttcttt tgcttctttg ccgtgagtgt atttcgttac atagacatat
     1921 tccacgcctt ttaattctct aaaagtgata gcatctgtcg tcagaccttg tccgcggaca
     1981 aatccttcag ctgctttcgt ccagtttcca tctgcgtcca aagcaatttt tttggctggt
     2041 cctttgactt cttcttcgct gtctgcttga cgttctggaa tgtttgttac tttgattgct
     2101 aagcggcgcg gtgtagaaaa cgtttcgatc gtatcataag tcaattggtt ttcatctaaa
     2161 aattttgctg ttttttcttc taattgtttc atgcttggtg tgactacatg agcaggcatt
     2221 tcttccagcc cgatttcaag taataagtct tttgccatct tattcagcct ccttcacgtt
     2281 ttctggattt gctgtgtttt tattcaataa agggaatcct aatttttcgc gttctgccac
     2341 aaagattttg gctacagcac gagccatgtt gcggatacgg gaaagataac ctgcacgctc
     2401 tgttacggat actgcaccgc gagcatctaa taaattgaat gtatggctac atttcaaaat
     2461 ataatcatac gctggatgaa caagattttc atcgatacag cgtttagctt ctttttcaaa
     2521 acgatcaaaa ttgcttagta gcaggtcttg gtcgcttact tcaaaagaat atttagaatg
     2581 ttcatattct ggttgtttga agatctcacc atatttcacg ccatcagccc attccagatc
     2641 ataaacactt tcaacttctt ggatataaga agcaagacgt tctagtccat aagtgatctc
     2701 agaagtaact ggtttacaag gtagtcctcc gacttgctgg aagtacgtga attgtgtgat
     2761 ctccatccca tcaagccata cttcccagcc cacaccggca caacccatcg atgggttctc
     2821 ccagttgtct tcaacaaagc gaatatcgtg ttctaatgga tcaattccta aaagacgcaa
     2881 gctttctaaa taaagctctt ggatattttc aggtgaaggt ttcattacga cttgaaattg
     2941 atgatgctga tacaaacggt ttgggttttc tccataacgc ccgtcagctg gtcggcggga
     3001 tggctctaca taagcagcat tccatggttc cggtccgatt gcccgtaaga atgtatatgg
     3061 gctcatcgta cctgctcctt tttctgtatc ataagcttgc atcagcatgc agccgtttga
     3121 tgaccaaaat ttttgtaatg ttaaaatcat atcttggacg gtcaattttt tactcatctg
     3181 ttttgcccct ttcaattttt aagcgttgct gtcaataaga atacaggaaa gaaaacgaca
     3241 aaaagtccct atgtctgatt acagacatag ggacgaataa tttcgcggtt ccaccctact
     3301 tccgaataaa gattcggcag cttcatttat cgtactcgca agtgcctctt caaaaatcgt
     3361 ttgggtccgg cttccactct cccgaactcg ctaaaccacg tgttttttac tgatcttggt
     3421 caatgtacgt attcattttg cagttttctg gtttgtataa tagatcctct cagtgaacga
     3481 tctatttaat ctacggttaa taatagacgg aagtccctgt atttgtcaaa tagttttgat
     3541 ttttttgttg tatctttcat ttattcttct ttttcaggta tctctttttc aggcatattt
     3601 ttctctggcg tctttaatac gtgtgcccaa gacttcattt gatcaatgaa ctttttgctt
     3661 tttagatgaa taccgacata ttcatcatac aactggtcaa tgaggtcacg gattgcctgc
     3721 ttcgtttctt ctttcaagtg gattccactg atcttatcat aagaaatagc cgaaaacatt
     3781 cgaacgaaat aaacagcgcg aggatctgca tgataacggt gttcgtccat tggccagtgt
     3841 ttctcgcata tcacaccgcc atatttggaa gaatagtcaa attttccttt tgtttccccg
     3901 catacgacac agtgcgtcca aattggcgcg attccgaagc gttgcagtat ttgcacttca
     3961 aagatattcg tgatgatttc tgcatcatct ccttggtcca tccgctgcag agcttgttgc
     4021 gtaaattgga ataaatgcgg gtcatattga tgatcttcga ttgctgcatc tactagattt
     4081 agcaaatacg taccataacc gctcaaaaag atatcttctt ggatgttccg aaacgcatgg
     4141 acgtctttga cactgcttaa aaacgacaaa ccttcacttc gaaaattacc gatatagact
     4201 gcttcggtaa aggggagcaa agccggagct aatggctgat tttgccgatg gattcctttg
     4261 atataaaaca tcattttgcc agaagattcc gtaaagattt tgactaattt atctttttct
     4321 ttgtaattac ggctagataa aatcaagcct ttcgattcta caagctggct catattctcc
     4381 ctcctaaaaa ataaatctgg aaaagcagat tctgccgatc cagattattt atgtttcgat
     4441 cttccgctgt ttttctcaac gtacggattt ttatccagtt tatttctata cctaacctat
     4501 aagaagtcca gaatatcagt actcgtcttt tcggtagccg aagtcttgca gatagacttt
     4561 tttgtctcgc cagtcttttt ggactttgac ccaaagttct agaaatactt tgtctcctaa
     4621 aagattttcg atatcctttc ttgctttcgt tcctacttgc ttcagcattt ttccgccttt
     4681 gccgataatg attccttttt ggctatctct ttcgacgatg atcgtggcct ggatatggat
     4741 tttatcattc tcgtttcgct tcatcgaatc aacaacgact gcgacagaat gaggcacttc
     4801 gtctcgtgtt aaaaacaaga ctttctcacg gatcaattca gaaacgatga agtattcagg
     4861 atgatcagtc acttgatcat ctggaaaata ttgtgggccc tctggcattt cactgactaa
     4921 tacatccatt aagcgttcga cattgtttcc ttctgtagca gagatcggaa tcacttgtgc
     4981 aaaaggcatc tgcgtcgaat aatcttcgat gattcccagc aactcgtctg gatggatctt
     5041 atcgatttta ttgataatca gataaacagg agaatgcacg ttttttagac gttcaataat
     5101 aaaatcgtct ccccggccac gtttttgatc agcactgatc ataaataaga ctgcatccac
     5161 ttctttcaac gcactgtaag ctgcttctac cataaaatct cctagacgat gctttggttt
     5221 atggattcct ggtgtatcga taaaaatcag ctgagcttct ggtgtagtat atactccttg
     5281 gattttattt ctagttgtct gtgccttgtc actcataatg gcaatttttt gacccacgat
     5341 tcggttcaat aatgtggatt ttcctacatt cgggcggccg acgattgcga caaaccctga
     5401 tttatgttct ggcatatacg ttctcctttt taattaaaca acaaatgata tattttaggg
     5461 acaaataaaa tcaacccaat gatgacagca aaacaagcgg tcactaaaac tgctccagct
     5521 gccatatctt tgattttttt cccaatcgga tgaaaatgaa aatccgtaaa catgtccact
     5581 acattttcaa aaacagtatt gatgatctct actatccaaa cgagaaaaac agctaaaagc
     5641 agccataacc actctatccg atcaagctgg aaaatcacgc ccataaataa agcaaggata
     5701 ccgaacgcaa catgtttcct catattgcgt tcttcctcaa aaacggtttt gactccttgg
     5761 acagcaaatt ccacagacat caggaaatgt ttgtttttct ctacgttttt atcttttgag
     5821 tccataggca tccaagatct ctttctgtaa accaaacatt tctttctcat cttctggtgt
     5881 catatggtca tacccgttga tatgcaaaaa tccatgtacc gctaaaaagc caagttcccg
     5941 atcatatgaa tggccgtaag cttctgcttg ttcagcagca cgttcaatag agatcatgat
     6001 gtctcctagt acacgaggta atggattctc tccatcttca aaaatcactg ggatttcgtc
     6061 ttcgccttct tcttccagag caaaactgat gacgtctgtc ggtgcatctt ttccacggta
     6121 atcacgattg attacttgaa ttgccgcatt gtccatgaaa gtaacagaca tttctgtttc
     6181 ctctggcagc tttaaataag atgctgcaaa ttgcagcaag tcatcgattt ctttcatttt
     6241 ctcttctgag acttggtttg tttcatccat aaaggtaata tccattatac atcgcttctt
     6301 tctattcttc ttgtacttcc tttatcttct caacttctgt tttcatttta ggatatttga
     6361 tccgagaatg gaaggtgcta cttaaaccgc tgattaatga tttttccact tttcggattt
     6421 ctttcaaggt cagtccacta tcgtctaatt gaccatcgga aatacgctct tcgataagat
     6481 tatggacaaa actttcgatt ttttcgctcg tcggatgatc cattgcccgt actgcagcct
     6541 cacaactgtc agcaatattg acgatccctg cttcacgact ttgcggtttt ggtccaggat
     6601 aacggaattg ctcttctgtt acttccggat tgcgttcttt cgctttgatg tagaaataac
     6661 gcattagcgt ggttccatga tgctgtttgc aaatatcgat caccatttgc ggcatattat
     6721 aatcttccaa tattttggct ccatctgtca catgtccaaa gataatatgt ttactgtctt
     6781 ctggcaataa gaaattgtgc gggttttccg ctcctgaagg caagttttca acgaaaaagc
     6841 tagcatgttt gatcttgccg atatcatgat aatagcaagc tactcgagtt agcaaagaac
     6901 gtccgccgat ttctgctaca gcgttagcac ttagattagc taccatcata ctatgatgat
     6961 aagtacctgg cgcttcttcc agcaactgtt tcaataaagg atgatttgga ttgctgagtt
     7021 catttaatac aattacactg tcatcattga ccatcaattc aatatatggg tgtagaccga
     7081 tcgtcagcaa gaatgagaag agtgcaccag tgaacccgca gacgagcatt aaccaagtcg
     7141 ttgcatcacc aaagctcatt ccttgataga cgatcaaaat gacatccatc atcacaggaa
     7201 aaataatgac ccacatggca gcagataatc cttgttctga gatccgtttg cgtctgacaa
     7261 tcgttgccat gagcccagag aaaacataag acatcaagat aatcgttaga aagttcgtgc
     7321 caatcgaatc ataaaaaatg aaaagtgcgg atactgcttg gaaaatacca gccatgatcc
     7381 ctgctcttcg attcaagaaa aagcttaaaa caagtggtgc aaaagcagcg ggataaaaca
     7441 gcgggatata agtagcttgt tccgtttgga ataattggaa aaatttcatc agcactatgc
     7501 tgactgacat agctgtcaca tagaaaagca cgtattttgt tctttctcct gcattttgga
     7561 attgaagtga attataaatc agtacgccga tttgaagcaa aacagctaaa accatggcaa
     7621 ttagcggaaa aatcgaagtt gtttgattag tcagtcctag taattccaat tttttcatag
     7681 ctgaagcatc gatttgatta ccttcacgaa caatgatttc tccttggaag atcatcactg
     7741 gttgtacagc ttctcgtgcg gattgtttca attcttctgt ctttttctca ttcaatacgt
     7801 cattcactac gataccttga tccactaagt agcgaatcat ttgttgttgt gctggtgtga
     7861 cattcaaata ttggatttgc tcttctgctt cttgtttaaa agcttccaat tcactttcac
     7921 gaacttgttt gctcatctgt tcgtcaatga gcttcagact ctcatccctc acagtatgaa
     7981 tctcagtttc agtcatttca aatatcgtcg aataaaaatt gttcggtagt ttttgataaa
     8041 acacaacatt ttctgcattc acattttcaa aatttttctt cagcgcggcg atccgctctt
     8101 ctgtagtagg ctttgggata ttttcatctt cctttgcctt atcaatcttt tcgtcataag
     8161 atttgttgat ggcatcattc gtcttatcga tcaattcaaa tagttgcttg atccgattgt
     8221 tctggtcatc tgcaagatct ttttggtaag tgtattctgg agtgactgct tctgctgcca
     8281 gttttctttt ttgttcggtt tcttccgtgt tctcgatcgt tttgttcgca cggatacttt
     8341 cttctgctac ttggccttct ctgaaatcat tggccttttg tttgacacta ctaaatgtaa
     8401 cgccgcagat aatgataaaa aacaagaaca gcaggaaagg caatgctgcc tttccaagtt
     8461 tttcttgtag tttctgcagt gttttattca acatgtgctt atcctcctaa gcaaagctta
     8521 ttcttgatgg agatctgcct tttcataagc tttgatgatt tcggcaacta cagggtgccg
     8581 aacaacgtca cttgcttcaa aattaacaaa atcgattttc ttgatttgct ttaatgttcg
     8641 ttctgcatga acaagaccac ttgtcgtccc tcgaggcaag tctatctgac ttgtatcccc
     8701 attgacaatc atttttgatt gataaccgag acgggtcaag aacattttca tctgagcgac
     8761 tgtcgtgttt tgtgcttcgt ccaagataac gaatgcatca tccaatgttc gcccccgcat
     8821 gtaagctaaa ggtgcgattt cgattacgcc tcgttccatc aatcggttcg tatgatccaa
     8881 tccaaaaatt tggtataaag catcataaac aggccgcaga taaggatcca ctttttcttt
     8941 tagatccccg ggaagaaagc ctagattttc acctgcttcc acagcaggcc gcgtcaaaat
     9001 gattttttgc acttcacctt ttttcaaggc agcgattgct aatactactg caagaaaagt
     9061 ttttcctgtc ccggctggtc ctacgccaaa tacgatatca tgtttggcta ccgattcgac
     9121 atatttcttt tggccgctgt tttttacccg tataggtttt ccattacggt ctttgacgat
     9181 ctcttcctca tacatctcaa caaaataatc taatgtacct ttttgcccca ttttcaatgc
     9241 cgtcacaaca tcgggcgtac ttacgtggat tcctcgtttg atcaattcct gtaatgtccg
     9301 aatgactgag gcagccaagg aaatctggga ttgctcacca atgatctgaa tgatttctcc
     9361 acgtgtatgg atggttgttt ccgttgtttc ctcaatcagt tttatatgct tgtcgtgtgc
     9421 acctaaaagc atgctgacat catcagcagc cgtcaatcta atctctaaag aactagactc
     9481 ttccgtcaaa aatagacatc cccttttttt cgattaattc tttcctattt taccatactc
     9541 agaagaaact atatagcaaa taagaaaagg ttctaaaaga gttgcttaac cataagtacc
     9601 aagaaacgtt aaaaaactag ccgcttccat tctctaaagg tctcgtttga ataaaaaaag
     9661 ctgatcatgt aagctatgca taagatagag gacaagtatt ccgtctaact tagcaagtat
     9721 tctctgggta tttttcttgt ttcaaaaaaa gaaaacaaaa tatttgaaat attcttgcat
     9781 ttcaaaaaaa acaggtgtat tataacgtca acaaataaat atacagcaaa gcgtcgagga
     9841 aaacagtagg ctattcagtg atttattccc agtgagtcat gtaagtgaga aatgacaata
     9901 aatcgaatag acgaacatca tttccgagtc aatgtcgaaa acgtagatca ttgtggctgc
     9961 ctccattatc aggctagcgg ataactttat cgaaaagcgt ccgtgaatga agaagctttt
    10021 ttaagcgaaa aaaggtggta ccgttctaga atctagagcc cttttcccaa gtggaaaagg
    10081 gcttttttta tattttaagg tggtggtttt catggaatct gatattagtg gtatttctag
    10141 tttctgcaac gtttattttg ttactcaaaa tgataattat tggaggaaaa atttatggaa
    10201 gaaacaaaaa cttattcatg gaagcaatta ttgatggtca gttcacttat cttcgggatg
    10261 ttcttcgggt caggtaatct gatctttcct gtacacttag gtcaaatggc tggatcaaac
    10321 tggatcagcg ctggtgtagg atttgccatc tcaggcgctc ttttcccatt attagctatc
    10381 ttagctgtag tagtgacaaa aagtgatggt ctgtatgact tagctaaacc tgtcggaaaa
    10441 tggtatgctg ccttgttctt gatccttgtt catttaacga tcggaccatt ctttggtaca
    10501 ccgcggacag cagcaactgc ttttgaaatg gctgctaaac cctttttgcc tgaaaaatat
    10561 gattcattag gtatgttttt attctctgct cttttctttt tacttgctta tttccttact
    10621 gtacatccaa cacgtttggt caaatatgtc ggaaagtatt taaactcctt gtttcttgca
    10681 ttattagcta tcatttttgt cgtttcttta ttccaaccca tggggaatct aaaccaatct
    10741 gctgcagatt cttatcagac gaacgctttg acaaatggcg ttttagaagg atacaacacg
    10801 gttgatgcag tagctttact tgctttaagt gtcacttttg tccatgcagt tcgtgatctt
    10861 ggttttaaag ataaaaaagt cacagaatta acagcaaaag ctggtagcct gagcgttgcg
    10921 ttagaagtca tgatttattt tggtctggtc cttctcggtg cgatgagttt gaataagatg
    10981 tctttatcag aaaatggcgg aactgcactt tctcagatca ctaattttta tttaggtaac
    11041 ttcggtgcta gcttcttagg ggtaatggtc acattaggtg tcttcactac tgctatggga
    11101 ctcgttgttt cttttgcaca agatttccat aaactgttcc ctaaagtcag ttatatgaca
    11161 tggctgcggt taacgacttt tgtctctttt gtcgttgcga atgccggatt ggataatatc
    11221 atccaatggt ctttaccagt attgatgctt ttgtatccat tgtcacttgc gttaatctta
    11281 ctttccttaa cagcaaaatt cttccaaaag agtccatttg tctatcaagt aacgatgttc
    11341 ttttctgccg ttccagccgt attggatatg ttggcaaatt cgcctgcatt agtgagtcag
    11401 caaactgtcg tagcaagctt actaggattt tatcatcatt atgttccttt tgctgcatta
    11461 ggtcttggct gggtcgtccc aacattgctt ggttacgctg gcagcttgct cttttattat
    11521 gtatatcgcg tatcaggata taaacaagaa gcaagtgagt taccagaaga ataaccaaat
    11581 caaacacaat ttaacataag agatattaaa caggaaaaaa acagctagaa gaaa
//
LOCUS       DACCXO010000090        11524 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.091, whole genome shotgun sequence.
ACCESSION   DACCXO010000090 DACCXO010000000
VERSION     DACCXO010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.091"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            327..533
                     /locus_tag="IVY70_002144"
     CDS             327..533
                     /locus_tag="IVY70_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP7959261.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(735..1736)
                     /locus_tag="IVY70_002145"
     CDS             complement(735..1736)
                     /locus_tag="IVY70_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7959262.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1741..3654)
                     /locus_tag="IVY70_002146"
     CDS             complement(1741..3654)
                     /locus_tag="IVY70_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP7959263.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3822..4328)
                     /locus_tag="IVY70_002147"
     CDS             complement(3822..4328)
                     /locus_tag="IVY70_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7959264.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4488..4928
                     /locus_tag="IVY70_002148"
     CDS             4488..4928
                     /locus_tag="IVY70_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP7959265.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4954..6111)
                     /locus_tag="IVY70_002149"
     CDS             complement(4954..6111)
                     /locus_tag="IVY70_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959266.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6114..6470)
                     /locus_tag="IVY70_002150"
     CDS             complement(6114..6470)
                     /locus_tag="IVY70_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959267.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6768..7742)
                     /gene="sgrA"
                     /locus_tag="IVY70_002151"
     CDS             complement(6768..7742)
                     /gene="sgrA"
                     /locus_tag="IVY70_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP7959268.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7938..8777)
                     /locus_tag="IVY70_002152"
     CDS             complement(7938..8777)
                     /locus_tag="IVY70_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP7959269.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8962..9849
                     /locus_tag="IVY70_002153"
     CDS             8962..9849
                     /locus_tag="IVY70_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP7959270.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10443..11138)
                     /locus_tag="IVY70_002154"
     CDS             complement(10443..11138)
                     /locus_tag="IVY70_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP7959271.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(11122..11520)
                     /locus_tag="IVY70_002155"
     CDS             complement(11122..11520)
                     /locus_tag="IVY70_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP7959272.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3435 a         2296 c         1726 g         4067 t
ORIGIN      
        1 ttaggttatc atttgtaatt ttatttcaca gttgatatga ttgttatgta aaataaaaaa
       61 gtttaggata catcgaattt ttggggaaat tcttttgggg gtatcctaaa tcttttctat
      121 tatattccca tattttctat tattggtcta gacgtatgat ggtaaacttt gtacctatct
      181 gattgctgaa tcattaattg cttatcatta gccatctttg tttcaagttc gctagatgat
      241 cattttattc acatcctaac aaacttgtca catttataga ttcttaatct atttatcata
      301 caattataaa aaaggaggtg ctgcttatga aagctatcca tatttctttg ctttttttcg
      361 ctagtgcatt gattttattt gctattcatc tttgttccca gccttatatc aataacaacg
      421 gaatattagt ggaaccagca ttcttttgct taccactcgg ctttttgtcc cttcttgcct
      481 ccgcaggctt cggatttgtt gcttttttta aacgatcaaa acaacaaatc tgacttataa
      541 aatttacgta aaagagatcg tgacaataat cgtgtgatat tcccaaattt cgaataaaag
      601 gtgccccttg ccatctctaa ctcctcagtc acaagttaca atattcataa accatgaaaa
      661 gcttgtttat tgaaattgct ccttgtgact tggagctaag tgacagatta caacctcttt
      721 tgcatcataa gactttactc cttgaaaaac attactaact tttgatatgt ttcttgcgct
      781 tgtttattgc tgaaatcaaa ctgatattca tggttcaaaa cagcattttt ctccgcataa
      841 tctacttttg tgactttcac ccccgctttc tcaaatacag aagttacttc ttccatttgt
      901 ttcgtaaacg tatttgtatt tccatccgtt aagaatgtgt tttgcgtcca acgatccgca
      961 tgtctgacca aggctaaact tttgaccttc tctgattgtt caaagtcctt attatcaaag
     1021 taagcccatc cacatttttc ataaagaaag ctagagattt tatcacttac ttgagtaagt
     1081 gcagaaggat ccaacagacc agacaaagaa ataaacttcg taatctgctt gttctccaaa
     1141 gtcggatcaa gatgatcagc ttttttaatt tcttcatttt gaagtgctaa caaatattct
     1201 ccactaattt gtgcacctgc cgaatttcca ccaatcgaaa tattcgacca atctgcttga
     1261 tcttttatct cttttttagc tgcttgaatg gcttcgttca attggataac tggtgtagga
     1321 tgttttgctt ccggtgatcg ttcataatct atatttacga tgatttgctg cgtgtctgaa
     1381 catagatatt ccaaataatc acgacaatca tttttatccc ctcctacata tgcgcctcca
     1441 tgcatccaga aaagaatcgg taattttttc ttttccactt tcggtgtgta gatatccata
     1501 ttattatttt tgtactttga aggataggat atttcttttt ttatatcgat ttgttcgatt
     1561 ttttgctgga aatcatctgg tcgggcatat acttgttctt tttcacttgt gtcaaattgt
     1621 tttctaacaa gcaagctaat cggctttggt gtgatttgat taatcaaaaa tacagtgact
     1681 aaaataacta cgatgataac tggtactgca atcactattc gcttgaacca tttcattcat
     1741 ttaccctctc tttcttcgcc aaatccatgt gataatcgtg ctaacagata aaaatccacc
     1801 tgaataaatg agaagcgaat gtttcttttc tccagttttc ggcaacattc ttttttttgc
     1861 atctgtcttt ttttgtactt tcactgtcat ctgaatcggt tgcgtatcat cacttgattg
     1921 atcctctcga ggttcattct ttcccttatt gtctgcattc tctgtcggat cttgcttttt
     1981 tggtggagct actgggattt ttttgtaata gaaaataata gttggtatcg tctcattaaa
     2041 aatcccttca gtcggtcctt caacatttgc taattcgtat ccttcgaatg tcttttgctc
     2101 tgtcatataa ttttctccat aagctccgta aagctggatt tcgtcctggt ctttgagtag
     2161 acttttattg ttttcatcca catactttac gtttatctga tcatggaaca ctactttcaa
     2221 aaagtccgtt acatgtttcg tgtaggcagc atggtcaact tcctgtgcct ttccatgccc
     2281 tgctccttct actaataaaa gatctttata ccctcctgat aactcatata atttttgcgc
     2341 gttagacaca ggtactacat catctgctgt tccatggata atcaatttag gcaatgaatc
     2401 tgacattcca atcgtaggta gagcagcttt tgtatccaaa tgcatctcct gatcaaaata
     2461 gtattgtttc aaaaggccca taaataaatc ttcatgctct tgatccccaa tgatgtctaa
     2521 ctgatttttt atggcatccg gtagtgcggg ggctactgtc tggctgtacg taaaacgtag
     2581 ttgttcatca attgatgcaa atccgcaatt ttcaataatt cctttgacat tgacaggaat
     2641 atctttcgct aatacagaca tcatcgtagc tgctcccatc gaaccaccat aaagtaaaat
     2701 cttttgagat ggccgctctc gaacttcttg atttatccag taaaggacgt catctgactc
     2761 atattgtcca taggtcacat aatcgcctcc gctattacca gtcgctctgg aatcaacaaa
     2821 taataaatta tacccggcct tataaaaatc attgtattct tctgtaacga tcccgttatt
     2881 ccaattccct cgaaatccgc catggatcat gactgtttta tctgaacctt gatcgacata
     2941 atatgcatat acatccatca ttttgccatc ttgtttgttt agtacagttt gatgaagttg
     3001 ttttgtattt ggatagaagc ctgtgttttc tgtaggcgtt gtcccatcat ctgtacctcc
     3061 aagcatcgac atcaacacac tgttttttgc aaaagcgata tcgtacaata gataagtagc
     3121 aatcagatgt ttgataatct ctttatctac accataatca ttaacagcaa gctcagtgac
     3181 taataactct attaattttg acttgatgtc ttctgttggt ccattaagta tttctgtcat
     3241 ctgtttaccg aagtaattca aatattcttt accattgaaa ttttcacctt taccgatatc
     3301 atcattttct gtcccattct ctttgtcact tactttagca gaagaggatt cgtcgtttga
     3361 catttttgta gctgttgctt cttctgtttg ttggttatct gtggtagcgg tcgtttcttg
     3421 atctgtgcta gtaacagatg tctcaaccgt tgtttcagta tttgtttctg tttgtgaagt
     3481 actcgatttc gtttcatttc cagcatcagt agtttctgtg gtttctacct cccgagattc
     3541 cgattcggaa gaaatgctct ctccatatac tgtccattgc gggacgcttg tacttacagc
     3601 aaataagatg accaacgctc cctttaatac ttgttttgtt ctcaagctat tcactccttt
     3661 ttatgtttct aggagaatta tatgtaaacg caaacatttt ttatacaaaa aataaatggt
     3721 ttatcccata aaaatagaac aggattattt tctcatttta cgtaataaag cagaatggat
     3781 aaaccagtat ccattctgct ttttttaaac gaagaaacaa cttattgctc caaaaatgtc
     3841 agaaatcgtt tatcccaaat tttctcgtaa aaagcgattg tttcctttgc tttcataaaa
     3901 ggattatcat aagttgtcgt tgctcctcta cacatcataa gctggtatcc taatccatga
     3961 gcaaacttca cagtcgtatc tatacaatac tctgcttgtg ccccacataa ttcgatcgaa
     4021 tgacagtcca attcagttaa taaaacactc aaattagtct tataaaatga attcgcatgg
     4081 gtttttctca caaaataact gtgctcagga atagataatt ccgacaatag ctcccatggt
     4141 tttgtttgtg gcactaattg agcatcttcg tgttgaacga aaataactgg tttcccaatc
     4201 gtttcgtatg ttttgattct tttattcact aatgctgtca ctgtttctaa ttcgtgtatc
     4261 ggcgaagaat caaaacatac ccctttttgt aaatcgatca ctactattgc gtcggctgtg
     4321 tttgacatac ttgtgccttc tttctttttt cttgatttaa tagtatttct tatctagcta
     4381 gttccattat acgtgacaac attttcttat tttacacctc taaatggcac ttttcgacta
     4441 tttcttcatg ttattatttc ataaatactt agctattgga ggaatgtatg atgattaaat
     4501 tgtatacagg ttctggctgc tcgtcttcaa gacgcgcaaa acgttggctt atcgtccatg
     4561 atataccgtt tatcgaacag aacttaattc gtcaaccact aaccaaaaat gaatttatcc
     4621 acttactaaa attgacaaac aacggcgtga ccgaaatgct ctctactcat tctattagtt
     4681 ataaaaatct tgttcacaga atagatgaac tcagtttgag tgaactatat attgagattc
     4741 gtacatcccc ttctttattg aaaaaaccga ttatctacga ccaatctgca aaacttttga
     4801 ctggatttag cgaagataaa atcaggatgt tcctttctaa aactaaaaga aagcaagagc
     4861 tcaacgaact cttgctttct ggtcatcttg tcgattttaa agaaaataat ctttttgcaa
     4921 tgtcttaatt ttggcataaa caatctgctt ttcttaatta acgaaatcca atttttccaa
     4981 tatggatcta tttttctcat tcctgatttt tatgatttca gctttcagac gcgcccaatc
     5041 attttttgta ggttgtccat tccagtaaaa tatctgtgta ttcgtttgat catatgcctt
     5101 tttgtcgata ggataatcgc taatgacaat atcaggtaac tgatcagcgg atgcactata
     5161 ttttattggg cgttcagtca tagataatat ccttttcttt gcccataatt cctgtgtttt
     5221 accatacttt gattgaatat agaatatgac aggttgttcg tgttttataa aaatatccct
     5281 gactaacata cagtattgaa aaatcaaccg tctgttcttt tcataataag tattaagctc
     5341 tggaattctg cgagctaccc cactaaaaaa ctgttgcatg attggataca tcactggaaa
     5401 tatatgctga aaatctctgt cttctgctga ttttccaaaa gcatccacct tcttttttgt
     5461 cccaaaaacc cttgaataca aatggatcat tgccaaattg acatatagat aatttttttc
     5521 tgaagttgta aaacaaatct tgaagttttt ttcaaaattt tctacaatag acgattcaat
     5581 ccagccccaa ctttcttgta ctttatctgg agtatccaac aaatcttctt ccaaataggt
     5641 gttcattaca ccaaacataa aataaaggta agcttgctct gttggagtga tgtgattcat
     5701 cgaccagaag tttgcttcaa aaaaaggctc tagtatgtaa tgcgtaaact cttcttttgt
     5761 tatcaatgga tgcacaaatt tgttcatctc ttctgggaca tacgtaatcg agtggccttg
     5821 tttcattcgt tgcaacgaaa ttcttagtag gctgatccat ttctttctat ctatactacg
     5881 cgtatatggc gcatgtttcg caaatttttc taatagttga cgtttatttt ttatacttga
     5941 taatatatca gaatgatttt cgtcgaatga ctgccaatac atcaagtgaa aaaagtatcg
     6001 gatacggtat tcttctcctt ctatcggatt accttttttc aagctgcaag ataaatgata
     6061 gtttttcaaa atagtattaa gttctcttaa ttttctttga atcgtacgca aacttatata
     6121 attttttgac taagattttc taaggattga taagtacctt tgtaaagtga atccagtaaa
     6181 atataaccaa tcgttccctc taaaagtata gaataaactt tactcagtaa aaactgagaa
     6241 gaaaactcga cttttatcat cttgttttct gaaagaaaag ataatttata ttctggatac
     6301 ctttcgcaaa acgattctgc ttgttgaatc accgaattca tcttagccag ggtgtagttt
     6361 agatctaaag cgatatcctt caccacatat aatttctcgt ctctttcata acaatagttc
     6421 aaaaattgat atacttgcct ttcattgata tcaataaatt cttcaaacaa agtacatact
     6481 cctttttatc tattatttag acttattccg acagctacat gaataacgta caaatagatt
     6541 cgttactgat ttttcagaga tactaccatg cgcagtgtgc attgtcaatt attcttgctc
     6601 ttattttcca tttgtgttcc tgttatttcc tgtttctcaa atatgttagt aaacaaaaat
     6661 agatctggca aagctacaac ttcgccaggc aatacagcac tctcgaatga aatattcact
     6721 caaaaatcta tcgttttatt caattttaga tctgttgctt ttattcttta tttcactcga
     6781 tggaagagag aacgaaaata gcttgctcct acaatcaaaa tgagtaacac acccatccat
     6841 gtaattagct tgttcaaggt tctactacca gtttctggta aatcgctttt tctattatct
     6901 gtttctttcg ctttttcttg atgatcaatt ccacgagaaa caaaatcagg ctgttttagc
     6961 acacttttta ggttgctccc tgcatttctc ttttgttcct tagttggtat gataggtacc
     7021 aaatcaagtt cattatttac ttttcctgtg ctgctttcgc tgctcgtgct tcctgtgcta
     7081 ctctcactac tcatgcttcc tgtgctactt tcactactcg tactctccgt actgctttcg
     7141 ctgctcgtgc ttcctgtgct actttcacta ctcgtactct ccgtactgct ttcgctgctc
     7201 gtgttttctg tgctactttc actgctcgta ctctctgtac tgctttcacc gctcgtgttt
     7261 tctgtactgc tttcactact tgtgctatcc gtactgcttg gcggtccaac aactgtccaa
     7321 ctgcttggtt tcaaattctc cgtgatcgtc atctgtccat cataaaaaac aatcaaatca
     7381 actttatctt tactcttgat ctcatcaatc gatagttctg cagaaatcat tattgtatac
     7441 tcaataccag cggtccattg tacgtctttt aaataaactt ccccaataac tttttctgaa
     7501 cttccattac catcccaacc atatgttaga acagcatgta catcactagc attcacgctt
     7561 ttgtttggtt tgactggaat agtaatgact tgttgtgtca tgtcataaac aatccgatca
     7621 gttttcacag taaatgaaag attctcattt gcccgttcat ttgcagaagt actgattcca
     7681 atcattgctg caattatact cagcccaaga ataacaaata atacggtcgc tgttttcttc
     7741 atttctatca ctctccatat aattaccttc tatttgataa aggaattata taaaatgaaa
     7801 atgatgagaa cacgacagtc agttgtgaaa atctaacaaa agatgtcgtg tattcgcttg
     7861 aatttgttta ctttttcgtt taatcaatga cagacttaaa aaagcaagac cgatatcagc
     7921 cttgctttct tcatctttta ttgataaaca gcagtgtatt tcttcatccg ctttgtttta
     7981 tatgcataga gtaacaaacc aacaatcaag actaacaaga ataatgtagt tgatatagat
     8041 gcttcagccc caaagtttcc cccagtaatt attgtatttt gcgattggat cagagtggag
     8101 aaaaaacctt gttcttgcgt tccgctgact ggtataccca aaatagctcc ttgtgtccaa
     8161 ttccaagtaa tatgagcacc aatagccgta aataaacttc ctgtactgta gataagtgcg
     8221 ctgaaaaagc aacctgctaa aaagatattg aaaactgcca gaaatgtcat gttaggattc
     8281 aatccgtgca aaagagcaaa taatgcagca gatccaatca ttgctattaa cggaccaaat
     8341 ctaagcatct gatgctgaaa aaagccacgg aataaaagct cttctgatgc ccccactgca
     8401 ataaataaaa taaaactcgt tatcagcggc acaatgtttg cgtttcctga atctaaccga
     8461 tacgcccccg tcaaatataa tggactagta ataagcagaa acactccaat tccaacaacg
     8521 agacctcccg ctaaataccc ccagtctttt cctcgaacag aaacaccata atcttctatt
     8581 ctttttcctt cgaccttact agcaagaaat aaagctagta aaaaaacagc taaatgtccg
     8641 accgtaccgg caattgctcc aatccattca ctatttccaa taaacatatc cgcaagtgca
     8701 ttattgacaa gaattcctaa aacaaaacca actaagacta ttaaaacaga tccaatcaac
     8761 cgaataatcc tgatcatctt tcatccctcc tttatattaa gtttaacaac ccttttcttt
     8821 cttgtctcgt gaaaactatg taaatgttct ccatcataga gagaatagat ttttacgaac
     8881 taagaatatc tacagcaacc ttacgaaaat gaaagactgt ctccctaatt cttctgttag
     8941 actgttttta ataaggaaag gatgggaaat tttttgaaaa aacaactact aattatgtgc
     9001 ctttcagcat gtgcaatttt actattctca ggttgtagca agtccaatga aacgattgca
     9061 acgtttaaag gtggaacact tacagttcag gatttttatg ataacccaaa agttaaatca
     9121 aataatcaaa cactattaaa aaaaatgatt gtcttcaaag catttgatga tgtatacgga
     9181 aaagagattt cttcaaagga agtaacaaaa gaatataatg aacaaatcaa aaaactcggt
     9241 ccaaactaca aggaacaatt aaaagcagtt ggccaaacag aagagacata taagaaactc
     9301 tttaaacaga tgctggcttt ccaatacggt ttgaaagcca atgtaaaatt aacagataaa
     9361 gatctagaca ctgcttggaa agaattctat ccagaagtta gtacacagat cattctattt
     9421 tcgactaaag aagaagcaga taaagcaaaa aaagaagcaa atgaaggaga aaatttctcc
     9481 aagcttgttc aagcttatgg aaaaaataaa acattaaagg aaactgatgg aaaaatgaat
     9541 tttgattcaa ccaatccaga gattcctact gaagtaaaaa aagcagcatt taaactaaaa
     9601 aacggggaag tttctgatat cattcctgta acagatccaa cgacttatca gcaaagctat
     9661 tatctagtaa aaatggtaaa aaaacaggat aaaggttcta ataaagacaa atacaaatct
     9721 gagctagaaa aaattgctac cgaagcaaaa actcttgata cggaattcat ggataaaaca
     9781 atccgtaaag tgatgaaaaa agacaatgta actattaaag atccttatgt aaaaaatata
     9841 ttcaaataaa caaatttgac ccaattgggg atgtggcaaa atttgagaag tgatttttgg
     9901 aaattccttc ttatttttga ggcaaaccac ttttgccaca acttcatcgg ggcctttttt
     9961 tatttgtacc aaaacaaatc actggatttg ttacaatcat gaggatataa tttaaaaaag
    10021 ttttttattt ctatcacatg aatcaatgcg ccattaagta gattctttca tgcttcccta
    10081 ttttttcact tgccgtatcg cttcaaaaac aactatcata aactcaaact cgactgaaaa
    10141 gttattttaa aaaagactaa aaatatccct tctggacttg ttcttcttat tctgttatac
    10201 tagagaaaca cgaaagatat ttttatgcga cacttttcat ttgtttttat gaaatttatt
    10261 caaaactggc gtaacttcag agttacaagg atgtattaga ggttagggaa tacatcgttt
    10321 aaatgagaaa taagtaatag ttagtaatag tcacccaaac tttaaattaa tgaatattga
    10381 atcgtcagat agcctcctcg agttttcgag aaggctatcg tttattttat ggagaaattg
    10441 attcagaagt aatcagcgac cttttttagt tcaccaatcg tttctgcttg gaaagtttgc
    10501 acccaatctt cttggaagcc cctcactgct ttatcaatcg agggcatctc taatacttga
    10561 tctagtagct ctttacctaa cgttatcgca tgagctccag atcgaatggc tgtagtgact
    10621 tgttcacaat ttttgaaact agctgctaat atttttgttg gtctttgcgt acgttcgatc
    10681 tcagtccgca acgaatcaat cactttcata gcatcaatgc cttgattttg catcctgtta
    10741 taataaggtg caatataatc tgctccatta ttaatagcta aatacccttg taattctgtg
    10801 taaatagctg taccggtaat caaataacct tcttttttca actttttgat cgcttgaagg
    10861 cctgcttgat tcaccggcac cttgataaac gtatcttttc ccagttcttc tatgataact
    10921 cgagcatcct ctatcatttc ttcttccgtt cttcctattg tctgaatatg gataggaatc
    10981 tgtccgatta tttttttgat ttctttcata tgcgagaaga agtttgacgc tccttctttt
    11041 ttcagaattg taggatttgt tgttacgcct gttaaattca aaaactgttg acaagaataa
    11101 atactttttg tatttgctgt atcaagcatg atttccatat agctttcccc cattctgatc
    11161 ctcttgatcg attttaatga ttgtcccctt tcttaaatgc ggcatttcct tatcttctac
    11221 atacattgtt cctggaagtt ccggctcatg agatccatca aattttatcg tgatatgtcc
    11281 taattcattc aaattagttt ggacaacatc tcctactgct gtgattacat acatttgttt
    11341 atcaaaacag agcttcatat cacaagttat ttcttttctt acctgattca attcaatctt
    11401 ataacaataa tctgttaatg tttccggtgc ttcatctcca aataaaatca ttattttttc
    11461 tttcctgaat acttctgctt ctataccaac atctgttaca accgtttcaa agatcgtcat
    11521 ctct
//
LOCUS       DACCXO010000091        11153 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.092, whole genome shotgun sequence.
ACCESSION   DACCXO010000091 DACCXO010000000
VERSION     DACCXO010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.092"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            115..1374
                     /gene="lysA"
                     /locus_tag="IVY70_002156"
     CDS             115..1374
                     /gene="lysA"
                     /locus_tag="IVY70_002156"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP7959273.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1361..2161
                     /gene="dapF"
                     /locus_tag="IVY70_002157"
     CDS             1361..2161
                     /gene="dapF"
                     /locus_tag="IVY70_002157"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP7959274.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2164..3045
                     /locus_tag="IVY70_002158"
     CDS             2164..3045
                     /locus_tag="IVY70_002158"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP7959275.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3042..3758
                     /locus_tag="IVY70_002159"
     CDS             3042..3758
                     /locus_tag="IVY70_002159"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP7959276.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3764..4459
                     /gene="dapD"
                     /locus_tag="IVY70_002160"
     CDS             3764..4459
                     /gene="dapD"
                     /locus_tag="IVY70_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP7959277.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4475..5476
                     /locus_tag="IVY70_002161"
     CDS             4475..5476
                     /locus_tag="IVY70_002161"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP7959278.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5473..6834
                     /locus_tag="IVY70_002162"
     CDS             5473..6834
                     /locus_tag="IVY70_002162"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP7959279.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6794..7945
                     /locus_tag="IVY70_002163"
     CDS             6794..7945
                     /locus_tag="IVY70_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP7959280.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7920..9089
                     /locus_tag="IVY70_002164"
     CDS             7920..9089
                     /locus_tag="IVY70_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7959281.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9264..10643
                     /locus_tag="IVY70_002165"
     CDS             9264..10643
                     /locus_tag="IVY70_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7959282.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10924..11153
                     /locus_tag="IVY70_002166"
                     /pseudo
     CDS             10924..11153
                     /locus_tag="IVY70_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3716 a         1948 c         2496 g         2993 t
ORIGIN      
        1 aaatatacta gacaattcag aaaattttgt ttaatattat tccaataaaa ttcaaatctt
       61 tatcaaatga aattccaata aaaacggaaa aaaagtgaaa agaggaatga aaaaatgaca
      121 gaattgataa ttggcggagt cacagcaagc acattggcag aaaagtataa gacacctctg
      181 tatgtgtatg atgaagaaaa gatgagacaa acgatgctgt cattcaagcg aggctttgta
      241 tcgaaacgtt ttgatacaaa agtgctttac gcctctaaag cattccaaac gattgagatg
      301 ctgcatttag ttcgtgaata tggattcggg ctagatgttg tcagtggtgg ggaaatctat
      361 accgctttgc aagctgattt tcctagagat actatctact tccatggtaa caacaagacg
      421 ccagaggaac tgatctatgc acttgaaaat gatttgttgc atattgttgc cgataatttg
      481 atggaagttg aattattagc agaattaagc caacgctatt taaaaaaaat ctgtatcatg
      541 cttcggttga atgtcggagt agaagcacat actcatgaat atatcgtcac tgcgcatatt
      601 gattcaaaat tcggcatgag ctatgaaagt aaagagtgcc aacaagccct tcagttgata
      661 aaagaaagca gctatctaga gttagaagga tttcatgctc atatcgggtc acaaattttt
      721 gatgttactg cttggttagc tgaaatcgac aaattgacga attacttaac cgattttgac
      781 caacctcttt ccttgaatct tggcggaggg tttgggattc gttatacaca aaatgatcag
      841 ccgatcccag tcgaagaggc actaaaaaaa ctgattgaat atacggaaga agctttggaa
      901 aaacgaaaac tgaccattcg tcaattactg atcgaaccgg gtagaagtat tgttggagaa
      961 gctggtacaa cactatacac gattggtttc atcaaaaaga caccacataa aaaatattac
     1021 tttgtagatg gcgggatgac agataatatc cgtccagctc tgtatcaggc agaatacgat
     1081 tgtgatttag cagcaaaact gtcggaagaa aaaacagaga aagtgacgat tgcaggaaaa
     1141 atgtgtgaat caggcgatgt ggtaattaaa gaagcttatt tgccgaaagc agaatcgggt
     1201 gatcttttag ctgtttattc cacgggagct tatggatact cgatgagcag caactacaat
     1261 cgagcgacac gaccagcagt tgtttttgtc aataatggcc aatcaagact gattgttcgc
     1321 agacaagcat tttctgatct actaaggggg gaagtttcct atgatccttc ataaatataa
     1381 tggctgcgga aatgatttta tcttattaga ttacgcagaa gacacagatt atagtcaatt
     1441 agccgcttct ttatgcacaa aagaaaaata tgatatagac ggattgatcg ccgtaaaaat
     1501 cgatccgttg gaaatgatct attacaacaa agatggcagc cgcgcgccga tgtgcggaaa
     1561 tggcattcgc tgttttgctc gatacgtaaa tgatcagaac atgatgaatg aacaggaatt
     1621 caacgtagag acattagcag gcacaagaaa aattacggtg ttggatccct caccttttta
     1681 ttgcagcgta gatatgggag tgccagatga ttctccggaa aaaatcaaag ctaatcaaac
     1741 ttatccaata aaaaaccagg aaatagagat caatggtgaa aaagtaacga tcactagttt
     1801 atttttagga acgattcata ccgttgtttt tgttgaaaat gcgcgtgagg aagtctggag
     1861 aaaaagaggt gaactgatct gtcatcatcc tttgttcaaa gaaaaaacca atgtcaactt
     1921 tgtagaagta gtcaatgaac aagaattgat cgtccgtacc tatgaaagag gagtaggttg
     1981 gacacttgct tgcggtacag gttgctgtgc ctcttatgtg gttgctaagg aaactgggaa
     2041 aatttcagca gatcaggtag ttgttcactt ggaacaagga gacttgatga tctctggtga
     2101 ggatgcgata caaatggctg gcccagcagt tcatgaatgg acaagaatat tggaggaatg
     2161 aagatgataa aaggttcgat cgtggcgctg atcacgccga tgaatgaaga gggatcagta
     2221 gattatgctg gtttggaaaa attgatccaa ttccatttag atgaacaaac agatggatta
     2281 ttagttttag gtacaacagg agaatcttct acgctcacac aatctgagga ggagcagatc
     2341 cttcaactga cagtaaagaa agtggctggc cgtgtgccgg tgattgcagg agctggaacg
     2401 aataatacga aagaaacgat tgaaaaagcg aagcattttg cttctttggg ggcagacgca
     2461 cttttggtca tcacccctta ctacaacaaa acaagtgatg caggactagc tgctcatttt
     2521 acagcaattg cagaagcctc accgattccg cttattttgt ataatgttcc ttctagaaca
     2581 ggcatgtcga ttccaatcca tgtattagta aatttagcag aacatcctaa tatcattggt
     2641 ttgaaagaag cctcaggaga tatggcttat gtgatggatg ccgctcggtt gattggagaa
     2701 gaattcttcc tttacagtgg aaatgatgat ttgatcctac cagtaatgag tgtaggagga
     2761 agcggtgtaa taagtgtatg ggcgaatatc caacctaaaa tagtccatga attagtaaaa
     2821 gatacgcagg acggaagatg gcagcaagca aaagaaaagc aattaaatgc acttgaactg
     2881 atccatgcct tgttcagtga gaccaatcct attcctgtca aagcagccat gtcattatta
     2941 gacttacctt ccggacctct acgcctgcct ttagtttctc tttcaaaaga gaaaaagaaa
     3001 caattagctc agcttttgtt aaaagaaagg acggttgaaa aatgaaaatc ggactgattg
     3061 gcagtggaca gatgggaaat agagtcgaag aagttgcaca agaaagagga gatacggtcc
     3121 ttcttttacg gacggcacct aaagaagcaa ctacgcttgc ttttacggaa ctgccagaac
     3181 tgcttattga tttttctcat ccagataatt tagagaaaat cctttcgtat agcttatcat
     3241 atcaacttcc gttagtcatt ggcacgacag gttatacaca atcacagttc caagaaataa
     3301 aagaacatgc caaacatgta cctgtgatgt atagtgctaa tttttcgttc ggcatcatgg
     3361 tgatgaatca actaatcaag caagcagctg caatgttgca aggctggcag atcgaactga
     3421 ttgaaaaaca tcatagtcgt aaaaaagatg caccatctgg tactgcaaat atgctgcttc
     3481 aaacaatcca agaagtgcaa aagctgcagc ccgtatacaa ctggcaaggg aaaaaaagag
     3541 aagaaaacca aattggtgta cacagtattc gtgcaggttc tttacctggt gaacatgatg
     3601 tattgtttgc tgctactgat gaaatattta cgatcaaaca tgaatctttt tctaatcgaa
     3661 tctttgctgc aggcgcagta gaagcagcgg actggttgaa agatcagtca ccaggttatt
     3721 acaaattaga agatttatta atggacaaag gggtgtaaaa aacgtggaag ctcaagaaat
     3781 catccgctac attcaaacgg catctaaaaa gacgcctgtg aaagtctatc tgaatgtaac
     3841 cgagccaatc gaatttaaag aaagtaaagt ttttggtgaa ggaagcagct tcgttgtttt
     3901 tggtgactat gagacgattg ctccggtttt agaagctgaa tcagaaaaaa tcattgaaat
     3961 cgaaatcgaa actgctgcaa gatacagcgc agttcctttg ctagatatta aaaaaattaa
     4021 tgctcgtatc gaaccaggtg ctattatcag agatcaagtg tcaatcggca acaatgccgt
     4081 gatcatgatg ggagcgatta tcaatattgg tgcagtaatc ggtgaaaaca cgatgattga
     4141 tatgggtgct gtcttaggtg gacgagcgac agtgggcaaa aactgccata ttggtgcagg
     4201 agctgtctta gcaggtgtga tcgaaccagc atcggccaaa ccggttatcg tagaagacgg
     4261 cgtactggta ggtgcgaatg cagtgattgt tgaaggtgtc catatcggaa aagatgcagt
     4321 cgtggcagca ggagcagtgg ttttagaaga tgtggcagca gaaactgttg tcggtggaat
     4381 acctgcacgt gtattaaaaa taagtgatga tcagacaaaa gaaaatacag cattgattgc
     4441 tgcgttacgc gaattataga ggagatggag aaaaatgcgc gaaatagata cctgtattgt
     4501 gggggcaacc ggattagtcg gccaaacgat gctcaaagtg ttagaagaat atgattttcc
     4561 agtaggacga ttgaaattat tagcttcaaa acgttcagcc ggtaaagttt gtgtattcaa
     4621 gaaaaagaaa cacactgtac aggaattgac agaaacttca tttgaaggat atgatttagc
     4681 gctattttct gcaggtgctt ctgtatccaa agagtttgcg ccaatcgcac gaagtcaggg
     4741 actatttgtc atagataatt cctcagcatg gcgggaagat tcggatattc cattaatcgt
     4801 tccagaggtc aatctggaag actatacatt gaatcggctg attgcaaatc caaattgttc
     4861 aaccattcaa gcggtacttc cgcttaaagc tttacaagaa gcctttggta tccagcgagt
     4921 caattattcg acctatcaag cagtttctgg ttccgggcaa aaaggcattg aagatcttca
     4981 tcgtacgcaa aatgacgagg aaccagtttt gtatccttat aatattagcc aaacggtgat
     5041 tccggaaatc gatcagcctt tggaaaatgg gtatacaaaa gaagaacaga aaatgataaa
     5101 tgagacccgt aagattctgc atcagcctgc tttgccagta tcagcaacct gcgtacgtgt
     5161 gccaatcgaa aatggacatg gagtctctgt ggcagttcaa ctgcaaaaag agtttacgac
     5221 ggaagaaatt agagagtgtc tcagccagtt tccagggatt caattagaag atgatctagc
     5281 agcacataaa tatccgacga gcattcttgc tagaggaaca gatatggttt atgttgggag
     5341 gatcagaaaa gacacttctt tagagaacgg attgttgttc tatacgactg ccgataacat
     5401 acgcaaagga gccgctgcga atgccgtaca gattgctgca gctttaatgc agaaaatagc
     5461 ggaggtggca agatgagtaa tacgctgaaa gtagcgaaat tcggcggaac atcgatgtct
     5521 catgaagagc aattcaaaaa agtccgtaca attgttcttt cagatcccaa tcgccaagtg
     5581 atcgttgttt ctgctttagg caaacgtacg tcagatgatg acaaagtaac agacttactt
     5641 tatttgattt atgcttacct tcagcatcat gttgattgga cacccctttg gaaaaggatt
     5701 tgtgatcgtt tcattcaagt aagagatgtt cttgccctgc agttacctat tgaagatgaa
     5761 ctagcacgca tacagaaaca actggaagac gaaaagataa ctgaagatta tttagtaagt
     5821 agaggtgaat atctgactgc tcaactgatg gctgagtatc ttggttacca gtttattgac
     5881 gcaaaagacc tgattttctt tgactatgat gggaaaatcg atcaggaaaa aacagcacag
     5941 gaaatcacaa aagcatttaa aagatatccg aaaatggttg ttccagggtt ttacggggcg
     6001 aaccctgcag gaaaagaaaa attattgggc cgtgggggaa gcgacattac cggcgcgata
     6061 ctggcgaaga tgttgcaagc ggaaagttat gagaattgga cggatgtttc aggaatcatg
     6121 atggctgatc cgcgaatcat cgatcatcct aaaatcatcg aggaaatctc ttttagagaa
     6181 ctgagagaga tggcttatat gggggctaac gttcttcatg aagcagctgt tttcccagtt
     6241 caagaggctg atatccccat ccaaataaaa aataccaatg atcctttcgc tcaagggaca
     6301 aaaatatcga atcacgaaat agaaaaggaa aatggactga caggaatagc tggacgcaag
     6361 gattttcttt ccatcacttt attcaaacgt catatgtccg atgaaatcgg gtttatctgg
     6421 aaagcaatga gcatttttgc taaacatgag atatcaatcg aacatattcc ttcagggatc
     6481 gacaatatcg gagtgatcgt ctcagcagaa gcgattgccg ataagttatt tctcatcacg
     6541 aaagaattaa aagaaacact tggcgtggaa gaaatcgtcg tgatcgaaga cttagctttg
     6601 atctctgtcg ttgggggtcc gcataaagaa ttgattggat tatctgggaa agtgttatcg
     6661 atattgaaca aattagaaat tcgcacatcg atcttatcac aaggagcaca ggagttgaat
     6721 ctgatcatcg gtgtaccgaa taatcaatat gaaacagtag tgaagggtat ctatgaaggg
     6781 atggtgaata cggatgctga aagaatatcg aatggcactc catcaaattc ctgaaatcgg
     6841 gtttcatgaa ttcaagacaa aaaaatattt atatgaacaa gtaaaaaaca gcgaggccgt
     6901 tatccatgaa atcggcgaaa caggactact cctttatttt gataatcagc aagaagaaac
     6961 gattgctttt cgaacagata tcgatgcact gccagtcaaa gaagaaacga atctctcttt
     7021 tgcttctaag catgaaggtt atatgcatgc atgtggtcat gacggacata tggcgatgct
     7081 tttagggttg actgacttta taactaaaca cattgaggat cttatttata atgtagtact
     7141 gatctatcag ccatcagaag aaatttccgg tggcgcacaa agcgttattc agtcaggatt
     7201 gttggataaa tatgaagtac aagcaatatt tggctttcat ctatggcctg gactagctaa
     7261 gggagaagtt ttctctcgac caggaccttt gatggcacaa agcagtgaaa cagacattat
     7321 cattaaaggc cagtctgcac atattgcttc tagtgaaaaa ggaatcgata gtctggaggc
     7381 agctgtacga tttttgaaga atgtttatga ttttgaccgg caattaccgg cagagtggaa
     7441 tcatctaatg aaatttggcc aaatccaagg tggaacgatc cgaaatgttt tagctggaga
     7501 agttattgta tccggaagta tccgttctta cagttcagat atgcaaaatt atttgaagag
     7561 gggattagac catttagcag aacagtttca ctctacagct actatttctt ttcgctataa
     7621 tgacggctat ccagctgtaa tcaaccatag aggcttatat gcagcactgt tagattcaaa
     7681 caaactacat gaattagatg ctccagttct tcaagccgaa gattttggca tatatacgca
     7741 acaatatcca tgtgtatttt tctttttagg tgtcggtgat acccctgctt tacatgatcc
     7801 gacgttcgat tttgatatgg atgttttaga aaatggtgta gaatggtaca aaacgattct
     7861 ttttacagaa aatcttcttg taaaaatcga tagcagttca aaagaggagc gagtaaataa
     7921 tgatgaattt atccaatcga tttagtccta ggctttctag aatagaagta tcaaaaatac
     7981 gacaattcga tcagcaaata tcgtcgatac cagatgtgat caaactcacc ctgggagaac
     8041 cggattttcc aactcctgaa catgtcaagc aagcaggaat agcagcgatt gaagaagact
     8101 tttctcacta taccggaatg agaggattag aggaactgcg ggaagcggct tgtatatttc
     8161 agcaacagcg ttatggatta acttatgatc cgcaaacgga agttttgacg acagttggtg
     8221 caacggaagc gattgcctct gcgttgttat ctgttttgga agaaggagac aaggttctta
     8281 ttccagcgcc tgcttattca ggatatcagc cattagtgga actagctggt gctgaattga
     8341 tcccgataga tacttctgac acagggtttg tctgtcagcc ggaacaattt gaacgagcat
     8401 ttgaacaata tggatcagct gtcaaagcag tcattttaaa ctatcctaac aatcctactg
     8461 ggacgacatt gtctgcaaaa caattgaaag caatcgctga ggttttaaaa aaatatccgg
     8521 tatttgttat aagtgatgaa gtatatgctg aattgactta tagtggaacg catatgtcca
     8581 tagctactta tcttccagaa caaacaatcg tgataagcgg gttatccaag tcacatgcga
     8641 tgactggttg gcgagtgggc tttatttttg ctcaaaaagc ccttattgac gaactaatta
     8701 aagtccatca atatctggtg actgccgcta caacgatgtc gcaaaaagct gcggctgaag
     8761 cgctaataaa tggggtgaaa gatagcgaga atatgaaaga gcaatatcaa acacgaagag
     8821 actatctggt ccagcaattg gcgcctttag gatttgaagt gacccagcca aacggtgcat
     8881 tttacttgtt ttgcaaactc ccagctgcca ttcaaactga ctcttggcag ttttgtgtag
     8941 aattagcgga acaggcaaaa gttgcgtgta tcccaggaat tgcatttggc ccagaaggag
     9001 aaggttatat ccggatttct tatgctagca gcatggaaaa attacatgag gcctgcaaac
     9061 ggatcaaaga ctttttaaca gaacagtgat tcatagattt tatcaaaaaa acaatcgtta
     9121 attttgcggt atattctgca aggagacgat tgttttttaa atttagttca aatgaacgcg
     9181 tttcttttga ttcataacta atgtaatgtt aagtttaaat tgtttagaat gattttaaaa
     9241 gaatattcaa ggaggtttca tcaatggaaa aaatcaaagt tgtcattgta ggtgcatctc
     9301 atggagggca tcaatcgatc ttggaactat tgacacgata cggtgaaaat gtagatatca
     9361 cgctatttga agcaggagac tatatttcct tcatgtcatg tggtatggag ctgtatttag
     9421 aagacaaggt aacagatgta aatgatgtac gtaatttcag accagaaaac tttccgcaat
     9481 ccaatgttca tattttaaat aatcacgaag taaaaacgat cgatgccgat aaaaaaacag
     9541 taacggttac tagcgcatca gataacgaaa caaaagaata tccttatgac aaattgattt
     9601 tgagttctgg tgtcaaacca aacaatctgc cagtaccagg agcagatcta gaaaatgtct
     9661 acttgatgcg tggatacaat tgggcaacca aaatcaaaga aaaattaaat gatccggcta
     9721 ttaaaaaaat cgctgtcgta ggtgcgggtt acattgggat cgaagcagca gaagcaagcc
     9781 gaaaagctgg gaaagaagtc gtattgcttg atgtgatcga tagaccttta ggcacttatt
     9841 tagatgctga gatgacagat attttagaac aacacctaaa tgaaaacggt attgaagttt
     9901 tgacaaatgc aaaaatcgaa gcttttactg gtaacggaaa agttgaggca atcaaaacaa
     9961 gtgaaaaaga aatcccagca gatctagtca ttcaagctgc cggtgtgaag gcaaatacag
    10021 aatggttaaa agggatcgtc gacttagatg aacgtggctg gatccaaaca aatgaatact
    10081 tgcaaacgaa tcttccagat gtgtatgcag taggagatgc tacacttgct tattctatcc
    10141 cagcacgcaa aaaaatgccg atcgctttag cgactgttgc acgtagagaa gcacgttatg
    10201 tagtaaaaca tctttttgaa gaagtaccaa acaaaccgtt cggcggagtc gtaggttcct
    10261 ctgcattaag tgtctttgac tatcattttg cagcaagcgg attgaatagc tttacagcag
    10321 caaaagcagg agtagatgta cgagtgtctt attatgaaga tacgttacga ccaaaatatg
    10381 taccagaaga acaaggaaat ccaaaagtgt ccatccaatt attctcggat ccaatgaccc
    10441 atcaattgct aggaggagca gtattatcta cctatgatgt tacaggtcaa ggaaatgtat
    10501 tggcactagc gatccaaaac aaactgactt tagaagaact agcagaagct gacttcttct
    10561 tccagccagg ctttgaccga caatggagca tgttgaatct agccgctcaa caagcgttag
    10621 gagaagaacc atttgtagaa taagaaaacg aaatatttga aaagaggttg tgaaaaagct
    10681 gccttgcatc aagtaataag aacagccaag taaaaacagc ttcttatgtt tttaacttgg
    10741 ctggacttat taccgcagat gcaagctttt gaacaccgtt tattttcaaa aaagggactg
    10801 tgacacgatt tgtgtaccga cccccaaaag ttagaccaaa aaatctaact tttggaggtc
    10861 ggtatttttt atggttaaat atacttttga acttaaatta aagatcgttc atgattattt
    10921 ggatggcaaa ggcgattgga ccgtaaaaat ccagatcaag agattgaagc ggaaatgctt
    10981 aaaattcggg agaagcacaa ggattatggc tgtttacgga tgaccaatga actaagaaac
    11041 cgcggtttct ctatcaataa aaagaaagtt cagcgcttga tcaagaagct aggaataaaa
    11101 gtaacggcat acacgcgtaa atcacgtcgc tacaattctt atcgaggaaa agt
//
LOCUS       DACCXO010000092        10670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.093, whole genome shotgun sequence.
ACCESSION   DACCXO010000092 DACCXO010000000
VERSION     DACCXO010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.093"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            224..1624
                     /locus_tag="IVY70_002167"
     CDS             224..1624
                     /locus_tag="IVY70_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP7959283.1"
                     /translation="MWLLNICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIY
                     YRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIA
                     CMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTD
                     LQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTW
                     VCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGA
                     TVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERP
                     HLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVND
                     SYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKD
                     SRYVTKKEINKKNENK"
     gene            1611..3245
                     /locus_tag="IVY70_002168"
     CDS             1611..3245
                     /locus_tag="IVY70_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959284.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            3420..4253
                     /gene="msrA"
                     /locus_tag="IVY70_002169"
     CDS             3420..4253
                     /gene="msrA"
                     /locus_tag="IVY70_002169"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7959285.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            4333..5322
                     /locus_tag="IVY70_002170"
     CDS             4333..5322
                     /locus_tag="IVY70_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP7959286.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            5611..6309
                     /locus_tag="IVY70_002171"
     CDS             5611..6309
                     /locus_tag="IVY70_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP7959287.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            6459..7775
                     /locus_tag="IVY70_002172"
     CDS             6459..7775
                     /locus_tag="IVY70_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7959288.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            7792..9207
                     /locus_tag="IVY70_002173"
     CDS             7792..9207
                     /locus_tag="IVY70_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7959289.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGHHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            9671..9991
                     /locus_tag="IVY70_002174"
     CDS             9671..9991
                     /locus_tag="IVY70_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7959290.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            10017..10334
                     /locus_tag="IVY70_002175"
     CDS             10017..10334
                     /locus_tag="IVY70_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7959291.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            10363..>10670
                     /locus_tag="IVY70_002176"
     CDS             10363..>10670
                     /locus_tag="IVY70_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7959292.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     "
BASE COUNT         3604 a         1656 c         2116 g         3294 t
ORIGIN      
        1 aaattttatg agaaatgctc aagaaattta tacaacaggt gatttatctt ttcatctttc
       61 acgaatcaaa gggttatctt ctatttttga agggccaatc aactatacaa catttaacaa
      121 ttatggagat ggactgaact atttttatcc ttttcttaca ataattcctg cagtcgtttt
      181 ttatggaatt tcaaataact taatattaag ttatgttcta tatatttggt tattaaatat
      241 ttgtacgatt ctgattagtt tttattatgg agaaaagttt tttaaaaaga tagatgctgc
      301 ttttttattt agttgtttat atacttttgc aacttatcga acagttgata tctattatcg
      361 atccgcaatt gcagaagcaa tcgctcttac gattatccca gctgttttat attatgctta
      421 tggattgata ttcaatagag aaaaaaaagt catcgcttta tctgtcagta taagtctact
      481 tgtgtatact catgtcttaa ctacactatt gtgtagtgca ttgattgctt gtatgattat
      541 agttcgtttg tttttaagca agcaaataaa aaacgatata aaatttgtgg tcgtgaattt
      601 agtaaaggct gtttttttga cttgtgcatt aactagctat ttttggtatc ctatgttgca
      661 gcagatgaat tatcaagaaa tacacagtcc cttcaaaaca gatttgcagc tagaagcact
      721 ttctatttca gatagtatca ttcaagcgtt aaataacgat attacaacat acactatggg
      781 gttattagga ttggttgcgt tgatttttcc tttattcata ctgaaagaaa tggagaaaaa
      841 agaaaaaatc atctatgttg gcgtagtttc tacatgggta tgcgtaacta atttatttcc
      901 atggtttttg tttcaaaaaa caccgataag cttactccag tttccttggc gtatcttagg
      961 tatccaaatc tttttttctt caatgatcat aacgcttgtt tttatgaaaa agactatgaa
     1021 taaaaaatat tcatggttaa tcattggcgc tactgtggtg tttctactcg tcacgtctat
     1081 agcagcaaaa gaaaattacg caaaagttat ccaatcaaaa cacggtcata ttgttatcaa
     1141 tgaagaaaca ctcccacgtt atacaacatc agatatggga ggattatacg attacgcccc
     1201 aacagaagcg ataaaagaaa ggcctcactt agaaaaacat gaagtaaaag taggagaatc
     1261 gtgggaaaaa gggaagtata aagctgcaga taataacatt acttacacag tggcaagcaa
     1321 caaaaagcaa aaagtgacag taccagtata tgcttattta ggtacacagg taaaagtaaa
     1381 cggtaaagtc gtaaatgact cttataaaaa acatggctta gtgaatgtgg atgtaaatgc
     1441 tggagaaaat aagatagaaa ttacaacaaa atatactcca actgctctca tggctttatg
     1501 gatcagtatt ctgacttatt gtttagtggt gtattcaagt tttaaacgat ttgcacctaa
     1561 taagttgaaa gattctcgat atgtgactaa aaaggagata aacaagaaga atgaaaataa
     1621 ataaaaaagt agtaatattg agtggattaa ttatagttag tagtatatat tctttcatct
     1681 ttttcaaaaa tacacagagt atttatacca ctggagatct ttcttttcac ttatctcgaa
     1741 tcaaggggtt atcgactatt tttaccagtc cgattaatta tgagacattc aattatactg
     1801 gatatggtgt aaattatttt tatccctttc tgactttttt tccagctgta atgttatatt
     1861 ggatgacaaa aaatttgatt gtaagctata ttatttacgt ttggttattg aacttgtgta
     1921 caacactcgt agcttatcat tatggcgagc ggtttttaaa acaaaaaaaa gcggcatttt
     1981 tatttagttg tctctatatt ttctctgctt atcggacagt agacatctac tatcgttccg
     2041 caattgccga agctatcgca ataacacttg ttatccctgt attattttat gcttatcaaa
     2101 tcatctctgg aaaagaagaa aagtatccgt ctgtaaagct agcattgagt atgagtttgc
     2161 tagtgtacag ccatgtacta tcaacattaa tgagtacagc attaattatc atttttattt
     2221 ttattcgttt ggtttctaaa ggtttcaaga atgcagattt tattgctata tttaaaaaat
     2281 tatttagtgc ggcaggaatg acattggttc tgacgagtgc tttttggtat ccgatgtttg
     2341 aacaaatgct ctaccaaaaa atcaacaaac ctagcgtgac aaatttatat gctcatgcat
     2401 ctaatgtgtt cgatagtctg actgaagcta tgaacaacga cttaactact tactcaatgg
     2461 gactagttgg tctgttatct ttatgtattc ccctgatttt atttaaaaaa ttaacaaaca
     2521 tagaaaaaaa gatttattat ggtacgtgtt tgacttggct agctacaact agtttggtgc
     2581 catggtatct tctgcaaaat acgccagcca aattgttaca atttccttgg cggatcttaa
     2641 gcttgcaaat aatattctct tctctgatat taacgatgat atttttcaaa aatagaagat
     2701 acaataagac aagagaacta ttttatttat ttatgactgt tttgttatta atcactttaa
     2761 ctgtttctag taaaaaaaat ttcaatcaaa aaataatttc tcagccggga catattgttg
     2821 taaacaaaga aacagtagag gcgtatacca ctaaaggaat ggattatttt ttatatgatt
     2881 attctcctat cgcttcattg gagaataagg agcatataat aaaacacgaa gcaaagattg
     2941 gcagtcaatg ggtaaacctt ccttataaaa caactggtag ttctatcata tatagagtaa
     3001 ctaatgagaa gagccaagta atcacacttc ctgtatatgc ttatttagga acgaatgtag
     3061 aaataaatca tcaacctgta aaggagcagg tgaatgctaa tggattggta caaataagcg
     3121 gagtcaaagg agttgctgat attaaagtaa gcacaagtta tacagtaacg actattattt
     3181 cttatcttgt tacattaact gcatatttat gtttattttt taaaatagtt ttccataaaa
     3241 ggtaaattaa cttttcattt ataaaataaa ttgattaaaa aggagaatct gtcgataagg
     3301 attctctctt ttttatacga aaaatctgta gggggatgaa gtaatcgctt atttcctcac
     3361 tttatgataa attacagcta aagtcactag cttgtcttct tcggaaagga ggagaatcta
     3421 tggatgagga acaaatattc attcaaacac tttataatct gattttgaat ccgaatacac
     3481 gagattggga acgaaaagta ttgattcaga caaagaatga cataagagaa aatatatccg
     3541 ttaaagaaca gttatcaaaa ctagaagcaa cactacgtcc attagctatc cgaatgaatc
     3601 tgacgcctga tgtaatggac ttttatttat tgattacgga aggatttgat aaggagcaaa
     3661 aatatgactt ttccaagcat gccatgcaag atgctgacta tcaagaacgt gcagtatttg
     3721 cagggggatg tttctggtgt atggtcgagc cgtttgaatc gaaaaaaggg atcctatctg
     3781 ttttgtcagg atacacgggc ggacatgttg aaaaaccaaa ttatgatcaa gtaagtggtg
     3841 gttacacagg tcatgtggaa gctgtggaga tcatttatga cacaagagaa ataagttata
     3901 gcgagttgct gacaatctat tggcaaatta ctgatcccac agatacattt ggacaatttc
     3961 aagatagagg gaagcaatat cgtccagtga ttttttatca agtcgaacga caaaaagaat
     4021 tagcggaaca atcaaaacag aaactagaca gttcaggaac tttccaccaa ccaatcgtca
     4081 caaaaatcga accagccggt acgttttggc cagcagaaaa ctatcatcag caattttaca
     4141 aaaaacaacc gaaacgttat aaaaaaatcc agcaagcacg taatcaattt ctgatctatc
     4201 aacgagtaaa aaacaaatgg caaaaaaata tacgaaaaaa tcattttgat taactccttt
     4261 tctttaaaat gtagtaaact aaaaatagaa aaaagaaagc gttttaaaaa gatttgatga
     4321 ggaggagaaa aattgtatat tgcagatagt gcacgttatg aaaaaatgga atatcgtaaa
     4381 tccggtcatt caggtttgaa attaccgatt ttatctctcg gattatggca aaattttggt
     4441 gattatgatc ctatccataa tcaaagagag attttaagag gcgcattcga catgggaatt
     4501 acccatttcg accttgcgaa taattatgga ggccctgctg gagctgcaga gaaaaacttt
     4561 gggcgtattt ttagagaaga ttttcaagcc taccgtgatg aactgattat ttctagcaaa
     4621 gcaggttatc atatgtggga agggccatat ggggaatggg gctctcgtaa atccatcatt
     4681 tcaagttgtg accaaagtct ccaaagaatg gggctagatt atgttgacat cttctatcat
     4741 catcgcccag accctgatac acctttggaa gaaacagcag aagctttgat gcaacttgta
     4801 agacaaggaa aagcattata tattggtata tccaactata atggagaaga tacaaaaaaa
     4861 atgactgaaa ttttaaaaag aaaggaagca ccgttcatta ttcatcagat gcgctacaat
     4921 atgttttctc gtgcactact agaagacgat ctatctccag tattagaaga agaagggcta
     4981 ggggcaatca cgttcagtcc gcttgctcaa gggttgctga caaatcgtta tctacatggt
     5041 attcctgaag attctagagc acatcgtaag gaaataccat tcctttcaga agaacaagtt
     5101 ggatcaactc tggaaaaaat caaagcactg caaacaatcg ccgtatcacg aggacaatca
     5161 ttagcgcaaa tggcccttgc atggaatttg cgacaaaaat ctgttacaag tgtactggtc
     5221 ggagcaagtc ggttaagtca gttacaagaa agtgttcgaa tgatggataa tttagatttc
     5281 tcaccagaag aagaattgag aatcgaccaa attctagaat agaaacgatg taatcaaact
     5341 ttttatccat atattaaaaa aaacaaaaga tgacatcaag aatcgaatga agatcgtctg
     5401 ttattgaaca tcatattttt tgtcgttcaa aataaaaatg atcgaaggct aggagtgaat
     5461 tttatcctag tcttttttat ggtaaatatc ttcttcgttt gtatgtctat gaacggatta
     5521 atcaattttg tatacaaaaa atcatcagat gattcaaacg tgttataata attacgaaaa
     5581 aatacatcag atgaaaagga ggtatgtggt ttggaagaaa ataaaaccct agtagaagta
     5641 actactgaca agatccttag atatttaaag gaaatgcagt tgcaagtagg agataaactg
     5701 ccgaatgaat atgaattgtc cgattattta gaggtggggc gaagcacggt gcgagaagct
     5761 gtgcgggctc tagcttccag gaatatcctt gaagttcggc aaggagcggg gacatttgtc
     5821 agtgataagc gagggattgc caatgaccca ctcggttttt ctttgattga agatcaggat
     5881 cggatgatac gagacttatt tgaactgaga tatttattgg agcctaagat ggcaggtttg
     5941 gcagcggaat atgcaacgaa gaatcaaatc gtccaaatga aaaaattaac gcaaaagatt
     6001 gaaacttctt ttcgagaagg cacacaagaa catgttcaat tagacatcga ttttcatacg
     6061 atgattgcag aagctagcgg aaacgtagcc ttgctgcata ttatcccaat cattaatgaa
     6121 tcaattgctt tgtttaataa aaattatgat atccagcatc tgaaaatcga gacgatcgat
     6181 attcatacag agatcgttca ggccatcgaa aggcggaatg cacttgctgc gtttgatgca
     6241 atgactgtcc atatggcaat aaatcggaga gaattacaga aaataatagg agaaaaagaa
     6301 actaattgac agttcatctg atgtatttta tgataaatac atcagatgaa tttttgtttt
     6361 atatttggta aaaaagagga gtaaatagtt tctgttcatc gaaatgtaag cgctgactaa
     6421 tagacaaaaa gagatgacgt caaggagaag aagcaggaat gaggggatct ggaatgagtt
     6481 ttatggataa attagccaat gttttaggga acgtggcaac gagaatcaat aatttaaggt
     6541 atatcatggt gataaaaaac gcatttgctg cattgattcc ggtaattatt acgggtgctt
     6601 ttggcacatt gttttcagca atggtctttg actcggaaaa tggcttagca cagataagtt
     6661 tcttgcgttt tttagaagaa ttaaagccta tcgcatctgc catcagctat gtcacactaa
     6721 gtttcttgac catctatgct gtttttctga tagggatcga attagccaag ctgaataaag
     6781 tggatggtgt attccctggt atcatcgcag tgatgtctta tttatctgtt aatccaactg
     6841 tttatgaatt agtgaatgaa aatcagactg ttatagtaga aaacgtattg gctaaacaat
     6901 atacagatac gaaagggtta tttttgggaa tgttggtctc catcctttct attgaacttt
     6961 attgctggtt gaggaaacag aagaaactac agctgagaat gcctgatacc gtgccaacga
     7021 atgtttctgc tagtttttct gctttattcc cgacgattct gactgtatcg ggtatcgcgg
     7081 ctgcgggatt tatcatcaaa gaacttacag gaatgtacct ttatgatatc atttacaatg
     7141 tcgtacaacg cccattagaa ggtgtcgtac aagggttacc gggtattttg ctattgatgt
     7201 ttattgcgca agtcttttgg gtgattggta tccatggaaa tcaaatggtg aagccgatac
     7261 gggaaccgtt gcttttagcg gcaattgctg taaatacgga agcttttgaa gctggaaaag
     7321 aagtaccgaa cattattact atgccatttt gggatatgta tatgagtatg ggcgggtctg
     7381 gagtcacgat cggtttactg attgcagtat tgttagttgg aaaacgggaa gatatgaaac
     7441 aaatcaccaa gttatccctt gctcctggga tcttcaacat caatgaacca gttatttttg
     7501 gtatgccgat catgctgaat ccaattttag ctattccgtt tatcttaacg cctcttatca
     7561 ctggaacgat tggttatttt gcaaccgctt taggatttgc ggcgaaagca gttgtaatgg
     7621 tcccttggcc aatgccgccg atcgtcaatg cttatctggc aacagcagga aatatcggtg
     7681 cagttattac gcaacttgtt tgtattattg tttctatttt gatctatctg ccatttgtaa
     7741 aagtagcaaa tcgtgtggaa aatgatggga tctaaaaaac aggaggaaat catgaaaatt
     7801 actattccaa aaaattttat tcttggtgct gcatcttctg cttggcaaac ggaaggttgg
     7861 aaaggaaaaa aagaaggaca ggattcgttt atagatagct ggtataaaaa tgaacatttt
     7921 gtttggcata atgggtatgg cccagccgta gcaactaatt ttatggaaca ataccagcag
     7981 gatgtggatc tgatgaaatc catccatctg acacattatc ggacttctat caattgggct
     8041 cgttttttta ctgattatga gaactgtata gtagatgaag aatatgctca gcatataagt
     8101 gaaatgatcg actctttgat cgaagcagac gtagaaccga tgatttgttt ggaacattat
     8161 gaagtcccag cttttctttt ggaaaaatat gatggctggt cttcaagaaa agtagttgat
     8221 ttttatgcta agtatgctca aattgccttt gatcgttacg gtgatagggt aaaaaactgg
     8281 tttacattca acgaaccaat cgttccccaa acgcgttgct atttggatgc tgttcgctgg
     8341 ccgcatgaac aaaacacaaa aaagtggatg atgtggaatt atcataaagt tttagcaacg
     8401 gctaaagcag tagagattta ccgcagcgga aattatggtg gaaggattgg ctgtatttta
     8461 aatcctgaaa tggtctatgc tcgatccaat tctccagaag accaaaaagc tgcttatatg
     8521 tatgatttgt tttataatcg agtatttttt gatccgatgg taaaaggata ctacacagaa
     8581 gagttcctgg agttgtgtca aaaacaatcg atttatttcg atcctgcaac agaggattta
     8641 gaaacgattc agcatcatac agtagatttc cttggaatca atcaatatta tcctaaacgc
     8701 gttcgcgccc ctcgttatca atggaatgaa gagacacctt tccatcctga acgatattat
     8761 gaagtctttg atttacctgg gaagaaaatg aataattcca ggggatggga aatctatccg
     8821 aaaatcatgt atgatatagc catgtatcta aaagaaaatt atcataatat cccttggctg
     8881 ataacagaaa acggtatggg aagagaatat gaagaacagt atatggatga gaaaggaatc
     8941 gtgcaagatg attaccgtat cgaatttatc ggacatcatt tgcattactt gatcaaagca
     9001 acagaagaag gcgcaaattg cgaaggctat atgttatggg cattcactga ctgtgtgtca
     9061 ccaatgaatg catttaagaa tcgatatgga ttagttcgaa tcgaattgaa cgatgaacga
     9121 agcagaacat tgaaaaaatc tgcttcgtgg tatcagactt tgattgataa gagagaattg
     9181 gatttgcctg acgaaatcta taaataaata gaagtttttt gtagatgttc attgtattta
     9241 ctggttattt gaaataaaag aggttgtgaa tccagcgttt tgagctaata cccagggtca
     9301 gctgattgaa caccgtttat tgagaggttg tgaaaaagct gtcctgcatc aagtaataag
     9361 aatagcaaag taaaaacaac atcttatgtt tttaacttgg ttggacttat taccgcagat
     9421 gcaagctttt gaataccgtt tatttgaaaa aagttgtgac aagttttgtg tcacaacttt
     9481 ttttgtgcac gttaaaagaa aaaaggtaat aaaaattaat ttgtgtgttt agtgtgcgct
     9541 ttctttttta gtgtgtatgc aacttggtat agaccttaaa tgtgatgata tcaacttttt
     9601 aaagcgcttt taaagttggc acgctacttg cttatataca agtgtaagta tagaaaggaa
     9661 cgtgtttaaa atggaaaaga aaacaatcat gcttgtatgt tcagctggaa tgagtacaag
     9721 tctcctagtc acaaaaatgc aaaaggcagc agaagcaaaa ggaatggaag cagacatctt
     9781 tgctgtatct gcttcagatg cggataatca actagaatct aaaaatgtag acgttttact
     9841 tttaggaccg caagtacgct ttatgcaaaa tcaatttgct gaaaagctag cacctaaagg
     9901 cattccttta gatgtaatca atatggctga ctatggcatg atgaatggtg aaaaagtact
     9961 cgaccaagca gaaaaactaa taaacaaata ataaaaatta tcggaggata caataaatgg
    10021 aagatcaaaa aaatctagaa gccatcatgg gtctgatcat gtttggcgga aatgcaaaaa
    10081 gcgatgcaat ggaagcgatt gctgctgcga aaaaaggtga tttcacttta gcagatgaaa
    10141 aaatcaaaga tgcagaggaa tctttagtcc aagcacatca ctctcaaaca gagatgctga
    10201 cacaagaagc acaaggaaat cacgttgaag tcacgctttt gacggtccac agtcaagatc
    10261 atctgatgac atctatagct tttacagatt tagcaaaaga gatcattgat ctgtatcgtc
    10321 gattgaacgc gtaatctaca gtaaagaaaa gagggaagat ttatggataa atttgttgca
    10381 tttatggaga aacattttat acctgtagca tcaaaaattg gtgcacaacg gcatttagtt
    10441 gcgattcggg actcatttat ggtgagtatg ccgttaatga tcttaggagc attagcagta
    10501 atgatcaata acttaccgat tcccgggttc caagaattga tgaactcaat ttttggtggt
    10561 gaatcatgga aaggattcgg ggctgctgct tggaacggta catttgccat tctttctgtc
    10621 ttgattgctt tcttgcttgc ttatcattta gctaacggct accgaaaaga
//
LOCUS       DACCXO010000093        10501 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.094, whole genome shotgun sequence.
ACCESSION   DACCXO010000093 DACCXO010000000
VERSION     DACCXO010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.094"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(176..661)
                     /locus_tag="IVY70_002177"
     CDS             complement(176..661)
                     /locus_tag="IVY70_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP7959293.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            1141..1782
                     /locus_tag="IVY70_002178"
     CDS             1141..1782
                     /locus_tag="IVY70_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7959294.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1801..2436
                     /locus_tag="IVY70_002179"
     CDS             1801..2436
                     /locus_tag="IVY70_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7959295.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2454..3269
                     /locus_tag="IVY70_002180"
     CDS             2454..3269
                     /locus_tag="IVY70_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7959296.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3588..5024
                     /locus_tag="IVY70_002181"
     CDS             3588..5024
                     /locus_tag="IVY70_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7959297.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5213..7660
                     /locus_tag="IVY70_002182"
     CDS             5213..7660
                     /locus_tag="IVY70_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP7959298.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTELEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7890..8957
                     /gene="rlmN"
                     /locus_tag="IVY70_002183"
     CDS             7890..8957
                     /gene="rlmN"
                     /locus_tag="IVY70_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP7959299.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9150..10253)
                     /locus_tag="IVY70_002184"
     CDS             complement(9150..10253)
                     /locus_tag="IVY70_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7959300.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         3459 a         1799 c         2283 g         2960 t
ORIGIN      
        1 ttttcgtaga atcaataaat atggatttgg ctaactatgg aagaatactc gtattagttt
       61 tcagcgttca tactagttgt tacattgttt gattggccaa aagaatttct gatatatttt
      121 gtgctaaata aagaaaaaga cgcttgaatt gggaaataag aaaactactc tatcattaca
      181 aattttcata aactggcaga tgttctaagt aaacaagtat cttttcaaag tcggaaaaat
      241 ccataatcaa cgtagtggta ttgatatttg gatgaaagcc tacggtatct tgacgatcaa
      301 tagcagcatc gatcacgact tggatttttt tctctttgtc gtgcatcaat gctaatggag
      361 tcactgtacc tgctggaacg cctaggaggt caggaagctg ttcttcagaa acaaaagata
      421 agcgcttttc ttttaattgt tcagctagtt gttttaaatc ggcgtgcttt tcatcaggca
      481 agatgacaag atagatttgt tttccttttc ttgatttcaa taatagattt tttacttgcg
      541 gaccaggtag ggaaaaaggg acatttttga ctgacgtaat cgcaggatga tcgacttttt
      601 gaaaagagat acccagtttt tttagtagtt cataagcttc ttgttcggtt gcacgactca
      661 tcgcgcatca ccctcctttt tttattagtg tatcataaaa aagagttgac aaatgaaaaa
      721 taatcatgta aattacacgt aaagttaaaa caaatgacat cgaaaagaag agtagccgtt
      781 aggtgttttt agagagtctc tggttggtgg aaagaggcag cagttaaccg tgaaacacat
      841 cttggagtcg attttctgaa gcgtatagta ggaaaatccg gggagcccgt tacagctgcg
      901 gtcgttgcta gatgaccaga taaggctggc ttttccgtga ggagtcggta aaaaaaggtg
      961 gaaccacgtg attaacgtcc tttgatcttc ggatcaaagg actttttgtt ttcatctgaa
     1021 cggcaacgac tgtgtgaggt tttttctgca aggaaaaaat aaacagaggt ggaaccacgt
     1081 ataatacgtc ctcttggcat ttgccaagag gattttttta ttttaaagga ggaaaagaag
     1141 atggagtatt tgattgaagt tatgccgcaa ttattagtag gagcactcac aacattgaaa
     1201 ttatttttat ttaccttgat tggctcatta ccattaggga ttgttttagc attaggattg
     1261 tctttgcatt ttaagccgct acgctacttg cttcaaacat atgtatgggt tatgcgggga
     1321 acaccgctgt tgttgcaact gatttttatt ttttatgggt tgccgacgat tgggatcgtt
     1381 tttgatcgtt tcgaagcagc actcattgcc tttatcttaa actatgcagc ttattttgca
     1441 gagatctttc gtggagggat acaatcgatt cctcaaggtc aatatgaagc agcgcaagtt
     1501 ttgcaattaa gtccctttca aacggtgacg aaaatcgtca tcccacaagt gacaaagatt
     1561 gttttacctt ctgtagggaa tgaagttatc aacttggtca aagattcttc cttgatctat
     1621 atattaggtt taggtgacgt aatgcgtgct gggaaaatcg cgatggaacg cgacgcgact
     1681 ttactgcctt tgctaggcgt ggcaatcatc tatcttacat tgactggtat tctaacaatc
     1741 gtaatgaaac aagttgaagg aaaattaaat tattaccgct aactgatagg aggaagaaat
     1801 atgattgaat tgaagaaagt gaacaaagca tttggtgaaa aaaaaataat caaagatttg
     1861 aatctaatca ttccagatgg gaaaacatta gctatcgtag gtccttccgg tggcggtaaa
     1921 accacgcttt tacgtatctt ggctggctta gaaagttctg acagcggaga attttattta
     1981 gacggtgtac cgttcaaccc attcgagctt aaaatgaaag aacaagttgt aggagtcgta
     2041 ttccaagact tccagctatt tccacatctg aatatttttg aaaacattac cttagcacca
     2101 aaaatggtat tgaaacagac aaaagaagaa tacaccaaaa aagcagaaga attagcggct
     2161 tcattaggga ttagtgattt attgaatatc tatccttatc agctttctgg cggacaaaaa
     2221 cagcgggtag cgattgccag agcattggct atgaatccac gggtattggc ttatgatgaa
     2281 ccaacaagtg ctttagatcc tgaattacgc cagcaagtcg agcgtttgat cctatcattg
     2341 aaagaagatg gggtaaccca aattattgtc acccatgatt tagtatttgc agaaaatgtc
     2401 ggtgatcaat tactaaaagt ttccccaatt cattaagaaa ggagaagata gagatgaaaa
     2461 aaaccagttt tctattcgct ttgatcgcag gtcttcttct ttttgcaggc tgttcgaaca
     2521 aaaaaacttc agcagataat tgggcgacta tcaacgaaga gaaaaaaatc gttataggtt
     2581 tagacgatac atttgttcct atgggattta gagataaaga cgggaagctc acaggatttg
     2641 atattgattt agccaaagca gtctttgaac aatatgggat tacggtagac tttcagccca
     2701 ttgactggtc aatgaaggaa tttgaactga acaatggaac gatcgatttg atatggaacg
     2761 gctattccaa aacgccggca agagaaaaga aagtgcaatt tacaaaacct tatatggaaa
     2821 acgatcaagt gctgatcaca cctaaagaat caggaatcac aaactttgga caaatgaata
     2881 ataagagatt aggtgcgcaa aatgggtcat ctggttatga tgtatttacg aaacaaccag
     2941 aagtattaaa agagatagtc aaaaaccaag atgctgtttt gtatgatagt tttaatgaag
     3001 cattgatcga tctgaaaagc agacgggtcg atggtctgct gatggataaa gtatacgcag
     3061 actattattt gaaacaaaga aacgaactag aagacttcaa cataacaaaa ggttcttatg
     3121 agagtgagga ttttgcagtt ggtgcacgca agtcagatag tacattagtg aaaaaaatca
     3181 atgaagccct agatcaacta gagaaaacag gtgaattcca aaaaatatcc gataaatggt
     3241 ttggcgaaga tgttacgccg agaaattaac aaatagggga ctgtgacaaa agacgtgtca
     3301 cagtcccttt ttccgaataa acagtgttct cctgccaact ccttgtgact cggagctgaa
     3361 tggcaggctc acaacctctt ggtaaacggt gttcaaaagc cagtttttcg gtattaagcc
     3421 aaatttatgc aaaaatgtag aaagccattt tcacaaaatt tttcttacta ctcgaaacta
     3481 agcgactttt tcacaacctc ctttacataa ttttattttg ttgtggaagg ggtttcgtgt
     3541 tattctaaaa gaggaacgat tgaaggttat aggaaaggaa gagatgaatg gaacacaaag
     3601 aacttacctc atttccaaaa gattttctgt ggggctctgc ttctgcagcg tatcaagtag
     3661 aaggagcttg gcaagaagat gggaaaggag aatcagtctg ggatcgattt gtacggatac
     3721 ctggaaagac atttaaagga acgaacggtg atttggcagt agaccactat catcgttata
     3781 aagaagatat tgctttaatg aaagaacaag gtctgaaagc ttaccgcttc tctgtagctt
     3841 ggacacggat ttttcctaat ggacgaggag aagtcaatca agctgggctc gctttttatg
     3901 agagattgat cgacgaattg attgaaaacg atattgaacc tatgttgact ttatatcatt
     3961 gggatcttcc acaagcattg caagatgaat ataatggttg ggaatcacgt cagatcattg
     4021 aagatttcac tcattatgca gaaacattat ttgaggcatt ccgcgggaaa gtccattact
     4081 ggattagttt gaatgaacaa aatatcttca cttcattagg ttatttgtta gcagcacatc
     4141 cgccaggtgt gacagacccg aaacgaatgt atgaagttaa ccatattgct aatttagcaa
     4201 atgcatctgt catcaataaa tttcacgagt tagaaatgcc tggaaaaatc ggaccaagct
     4261 ttgcgtatac gccaaattat ccaattgaca gtaatcctga aaatgttctg gctgctgaaa
     4321 atgcagaaga cctgatggcc aattattgga tggatgtcta tatgtgggga aaatatccga
     4381 ttgctgccat gaggttctta gaagaaaaag gatgggcacc aacgattgaa gctggcgatg
     4441 cagaattgct agaatctgct aaaccagatt ttcttgggat caattactat caaacggcga
     4501 cgaatgcatt caatccatta aacggagttg gtgctggaaa aatgaatacg acaggaaaaa
     4561 aaggttcatc agaagaaact ggagttcctg gaatgtataa aaaagttgag aatccatttg
     4621 tcgaacgaac aaattgggac tgggagattg acccagaagg attaaggatc ggactgcgtc
     4681 gtatcacaag ccgttaccgc atgccggtac tgattacgga aaatggctta ggagagtatg
     4741 acaagttgac agaagataaa caaatccatg acgactatcg aatcaattac ttacaaagcc
     4801 acgtcaaagc catcaaagaa gcaatttcgg atggagcaga agtcctaggc tattgcacct
     4861 ggtcttatac agatttgctt agctggctga acggttatca aaagcgatat ggatttgtat
     4921 atgttgatca agacgaaaca caagaaggat ctttggaacg atataagaaa gacagttatt
     4981 actggtacca aaaactgatt gaagaaaatg gagagaacct ttagttcaca tacttaaaac
     5041 aagaaggtac ggctggatat ggccgtgcct tttcttgata aaatcattta gaaattaaga
     5101 aaaactgtaa aataagctga aaaatgaaag aaagcgtcag ttctttcaaa caaaacttta
     5161 gttcccttac ttttttttgt ataatgaaac ttgatgaaat gagggataat aaatggcaca
     5221 aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa acgaaaaagc aaaaacagca
     5281 gcaggaacaa atgctgacga ttggtgttgg gtttgccttg attttatttg ctgtcttcgg
     5341 ctttttaaaa ttaggttttt taggaatatt gattgcaaac ggctttcgga tcattgcagg
     5401 aaatacttac caaatcctct gtcttttatt agccgtatta ggattttgga ttgtcattaa
     5461 gaatacagag ttttcaattg gtaaaaaccg acgctggttt ggcggcatac tcttttattt
     5521 cggtatctta ttattgttac atgcccattt atttggcaaa ctccatactg gggaaccaaa
     5581 tatcatgggg acgacatggg atttgttagc cagtgatata aaacaaagtc aagtagacaa
     5641 taacgttggt ggtgggatga ttggagcaat cctttatcat tttacttact tcttgattgc
     5701 acagcctgga agttatttag cagcagtgct attactggca ggaggagcat tcctgttcag
     5761 taatctggaa ggctatcagc tgttaaatgg tatccaatca attggagaac gagtacagga
     5821 attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa gcggcaaagg aagagagaat
     5881 gaaacagcgc gcagaagcga aagaagcaag gcgcttagct gcaaaagaag ctgcagaaaa
     5941 agaagctgca gaatatgaga agaaaaaagc ggtgagccag caaagggaaa atactgctga
     6001 tgaatggcag gaaccagaaa atacagagct ggagcagcta agttttgttc caatcgacag
     6061 ttttcaagaa aatatccatc ctgcgaatct agagaaacca gtaccagata caccaaaaca
     6121 gaccaataca gcagaaggtt tttcagatga gttgccagag gatgacggta cctcattaga
     6181 atttgagatt gaagcagaac aggaaaatca agactatgaa ttaccgacag ttgatttgct
     6241 tgattctata ccaactgtgg atcaaagtga tgagtataaa aaaattgaaa aaaatatcgg
     6301 cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc aaggtcgtca aagccagctt
     6361 aggaccatcc gtcacaaaat tcgaagtcca gccggctgta ggggtaaaag taagtaaaat
     6421 tgtgaactta acagatgata tcgcgcttgc tttagcggcg aaagatgtcc ggatggaagc
     6481 accgattcca ggtaaatcac tgattggtat tgaagtacca aatggaaaga tcagtatggt
     6541 ttcctttaga gaaatcatcg aagcacaacc aaaccaccca gataaattac tagaagtacc
     6601 tttaggacgt gatgtttctg gacgggtaca aacggctgat ctttcgaaga tgcctcactt
     6661 actcgtggct ggttcgactg gtagtgggaa gtcagtagca attaatggga ttatcacaag
     6721 tatattaatg cgtgctaagc cccatgaagt aaaactgatg atgatcgatc cgaaaatggt
     6781 tgaattgaat atgtacaacg gcatacctca tctgttgacg ccggtcgtga cgaaccctag
     6841 aaaagcagcg caagccttac aaaaagtagt acaagaaatg gaagaacgat atgaaaaatt
     6901 cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa tttgttcaac agaaaaactt
     6961 ggaaaacgga acgaaacatc caaccttacc attcatcgta gtgatcgtag atgaattagc
     7021 tgatttgatg atggtagcca gcaatgaagt tgaagatgcg attattcgtt tagcgcaaat
     7081 ggctcgggca gcggggattc atatgatcct agctactcaa cgtccaagtg tggatgttat
     7141 cacaggaatc atcaaagcaa atgtaccttc cagaatggca tttgcggtat caagcggtac
     7201 agattctcgg acgatcattg attctaatgg ggcagaaaag ctcttagggc gaggagatat
     7261 gctattcttg ccaatgggtg agaataaacc gatccgtgtg caaggagcat tcatttctga
     7321 ccacgaagta gaacgggtcg ttcaatttgt tacagatcag caagaagctc attatgaaga
     7381 gaaaatgatg ccgacagatg aagttgaaac gacaggtgct ccagaacagc cacaagatga
     7441 gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa acggcaagta tttcccttct
     7501 ccaaagaaga tttcggatcg gttacaaccg agcagcacgt ttagtagatg aactggaagc
     7561 tcatggagtt gttggcccgt cagaaggaag caagcctcgg aaagtattca ttgagcagga
     7621 agaaacatca gaaacgttgc cggacgataa tgacatgtaa aacttgtgtg atgaagggtt
     7681 gaaaaacgaa taaccaataa aaagacaagg catgatgata ccaaaagaaa aaatggtttt
     7741 tttccatctt ttctaatacg gtatgatgtc ttgtcttttt agtttttgtg agattaaagt
     7801 tctattcgat ttatttgtga agtattgcat taaggaaatg aatcatgtat agtatgtagc
     7861 gaaactttga gaaaaagagg gaaaatactt tggaaaaaca gtccatttat ggcttgacga
     7921 atgaagaatt aatcaattgg tttatagaaa acggcgagaa aaaattccga gctgcccaag
     7981 tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac agaaatgacg aatctatcga
     8041 aacaattgat cgaaaaatta tcggagcatt ttatcatcaa tcctctaaaa cagatggtcg
     8101 tccaagaagc ttcggatggt acagtaaaat atttgtttga actccctgat aaaaacatga
     8161 ttgagactgt actgatgaga caagaatatg gcttgtcagt ttgtgtgacg acacaagtag
     8221 gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct caaaaagaac cgtgatctga
     8281 ctgctggaga aattgttgca cagatcatga tggtccaaca ttattttgat gagcgcaatc
     8341 ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg tgagccattt gataattacg
     8401 acaatgtaat ggactttttg catatcatca atgatgccaa aggattggca attggtgctc
     8461 ggcacattac agtttccact agtggattag cacacaaaat caaggaattc gctaataacg
     8521 gcttgcaagt caatctggct atttccttgc acgcgccgaa taatgaggtg cgaacatcga
     8581 tcatgcggat caaccgaagt ttcccaattg agaaattgat ggaagctgtg gatgagtatt
     8641 tagagaaaac aaaccgtcga atcacgtttg aatatatcat gttgaatcaa gtaaatgata
     8701 gaccagagca tgcgcaacaa ttagcagatc tattaaaaga taagaaaaaa ttagcatatg
     8761 tcaacttgat tccatataat ccagtcagtg aacatgatca atattctcgc agtccaaaag
     8821 ctgatgtatt gagattttat gatgtattga agaaaaacgg cgtaaactgt gtgatacgta
     8881 aagaacatgg tacagatatt gatgcagctt gcggacaatt gcgaagcaag caaatgaaaa
     8941 aaacagttgc caaataatcg tatcctactc gttttttcaa ttaccataaa aggagcagtt
     9001 ttcttaatta aggaactgtt caacttaaat aagagaaaag attctatagc gaaagctata
     9061 aagctttttt tgacaagaaa aaccctcatc aagcaaagaa atgtcctcaa tttgatgaca
     9121 gcctgcttga tgggggtttt agcaattatt taactgattt tcttatatcc ttgctggctt
     9181 tcttgtgtag catgataagt ttctacgtca agaatggcct gtttaaattt ttctcgggtg
     9241 atttcataag gcacatgttt aatatcattt gttgccatgg ctttatctaa gattgcttct
     9301 aaatcggtcg tgacattgag attcatgtcg gttaacgata ctggaagttg gttctttttc
     9361 ataaacacat agattttatc aaacaaatcg aactgctgat ccatcatcaa taaggtcagt
     9421 acaccataac tgacagatgc tccgtgaagg tatccctcca ttttttctag aacagtcgta
     9481 ccgtaataga atgagtgtgc catagcagaa ttatagttat tgttaactaa aacagaaacc
     9541 aatccggtag taacaatgat atccgaaacg acttgttcaa ttgcatatga gggacgatcg
     9601 tcggcactat ctttcaatgc ttgcgtacca taagtcaaga gacgatcaat gcacattttt
     9661 ccgatttgga ttcctaatct atccgtgtag cctagatcat cgccacgagc agaaaaagta
     9721 gattcgactt ctttggataa agcatcgcca atacctgccc ataagtattt atcaggtgct
     9781 tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg gttttgaagg aaagaagagt
     9841 ccgtccatta cgccttcttc gttatagatg acacataccg cagtgacggg cgaacagttc
     9901 gaaccaatag tgggaaaggt gaagactggt ttagctagtt tttcaccaat gacttttaca
     9961 gtatcgcaag cacggccacc accgactgca aacaggacgt ctgctttttg gacactttca
    10021 tgctggatca actgttcaat atttccataa gttgaattac ccccgtacca gagactggcg
    10081 acaacctcta tttcctgtgt caaagctact tttattttgt tttctgcctt tgaaagtgca
    10141 gtcttccctc caatcagcac tacttttgtc ccatattctt tgacgattcc atttgctttt
    10201 tcatacgcat ctgttccaat ggtataattt ggtaagaaaa cactttgact catattcatt
    10261 tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag tgatcgatct gactgcggct
    10321 gtagatggag tagaggtgaa cctaagacag gataagtgat gaataaggtg gcatgaacac
    10381 cttgaaatca tcaataagta tcctaaaaac gaacaagtac aaaaacattg aaaacgacaa
    10441 taatgaattt ttgttcctct tagtatttct accgattccc tttgcttttt tgtaattatc
    10501 t
//
LOCUS       DACCXO010000094        10422 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.095, whole genome shotgun sequence.
ACCESSION   DACCXO010000094 DACCXO010000000
VERSION     DACCXO010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.095"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..438
                     /locus_tag="IVY70_002185"
     CDS             <1..438
                     /locus_tag="IVY70_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7959301.1"
                     /translation="QLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGNTMYTV
                     KAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVTGSKTY
                     TVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            865..3279
                     /locus_tag="IVY70_002186"
     CDS             865..3279
                     /locus_tag="IVY70_002186"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP7959302.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            3627..5180
                     /locus_tag="IVY70_002187"
     CDS             3627..5180
                     /locus_tag="IVY70_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP7959303.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(5561..7345)
                     /locus_tag="IVY70_002188"
     CDS             complement(5561..7345)
                     /locus_tag="IVY70_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7959304.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(7360..9081)
                     /locus_tag="IVY70_002189"
     CDS             complement(7360..9081)
                     /locus_tag="IVY70_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7959305.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLIIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            9181..9942
                     /locus_tag="IVY70_002190"
     CDS             9181..9942
                     /locus_tag="IVY70_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7959306.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         3400 a         1852 c         2095 g         3075 t
ORIGIN      
        1 caattgatcg ttaaaggtgg aacttcggca aatacaaata caggttcaac tacttctgca
       61 gcgaaaccaa atacaccgaa tacgtctgct acgacaagta catcatccac tggtaatacg
      121 atgtataccg tgaaagcggg cgagtcagtt tggagcgtag cgaacaaaca tcatatcacg
      181 atggatcagt taatcgaatg gaataatatc aaaaataatt tcatctatcc aggacagcaa
      241 gtgattgtga agaaaggttc atctcaatca acaaaccaac aatcagttac tggttcgaaa
      301 acctatactg tgaaatctgg agaatctgtt tggagtgttg ctgacagtca tggaattaca
      361 atggctcaac taatcgaatg gaacaatatt aagaacaact ttatttatcc aggtcaaacg
      421 ttgattgtag ctaaataaaa tagctgttcg aagaaataac gattgaattc cacaaaaata
      481 tttgttacta taaggctact taaatatgac aacacttgaa aaggaagtag taaaaagact
      541 gaagtttcag agagcgcatg gctggtgaga atgtgcacgg atgcttttga actcgccttt
      601 gagttgccct tgctgaatac ccgagtaagc aagcggcggt gacggccgtt acaacgttcg
      661 aggccttttc actaaggtaa aattaggtgg taccgcgtag attacgtcct ataaaaagag
      721 caatcttttt ataggatttt tttatttctt ttgtgaagtt tgagaaaaca aagcaaaagg
      781 aaataaaaat aaacagattc tgccggtttt ttcgttatat ttataataga aacaagtgtt
      841 ttcaatagaa caggaggaca gcaaatgagt tacaatcata aagaaattga gaaaaaatgg
      901 caaaaatact gggcaaaaac gaatactttc aacacccatg atgatcctga aaagccaaaa
      961 ttttacgcac tggatatgtt tccctatcca tccggtcaag ggctacatgt agggcatcct
     1021 gaaggttata cggcaacgga tatcctttca cgtgtgaaaa gaagtcaagg atacaacgtg
     1081 ctgcatccaa tggggtggga tgcattcggt ctgcctgctg aacaatatgc gctagataca
     1141 ggaaacgatc cagctgagtt tacccaaaaa aatatcgaaa cattccgacg ccaaatcaat
     1201 tctttaggat ttagttatga ctggaatcgc gaagtgaata caactgatcc ggagtattat
     1261 aaatggacac aatggatctt tacgaagctt tatgaaaaag gtctagctta tgaagcagaa
     1321 gtagcggtaa actgggtacc tgaattagga acagttattt caaatgaaga agtgatcgat
     1381 gggaaaagtg aacgtggtgg ctatgatgta gtcagaaaac caatgcgcca atggatgctg
     1441 aaaatcactg cttatgctga tcgcttattg gatgatctag aattagttga ttggccagaa
     1501 agcattaaag aaatgcaaag aaattggatc ggtcgttcag taggagcaaa tattgaattt
     1561 aaagtggctg gtacggataa atcctatacc gtatttacga cacgaccaga tactctattc
     1621 ggtgcaactt actcggttct tgcacctgaa ctagatcttg ttagagaaat tacgacccct
     1681 gaacaaaaag ctgctgtaga agcttatatc gaagagacag ctaaaaaatc agatctaaag
     1741 agaacggatc ttgcaaaaga aaaaacagga gtatttactg gtgcttatgc aatcaatcca
     1801 gttaatggca aggaaatccc aatctggatc gccgattatg tattatcttc atacggcaca
     1861 ggagccatca tggctgttcc agcacatgat gaacgtgact ttgaattcgc tcaaactttc
     1921 ggactagaaa ttcttccagt tatcgaaggt ggagatgttg aaaaagctgc ttacacagaa
     1981 gatggtacgc acatcaactc tgaatttcta gacggcatga acaaacaaga agcaatcgat
     2041 aaaatgaatg aatggctaga agaaaatggc gtagggaaaa aagaagtcag ctatcgctta
     2101 cgtgactggc tattctcccg tcaacgttac tggggtgaac caatcccaat catccattgg
     2161 gaggacggaa cagtcacagc tgtcccagaa gaagagctac cattacggtt gcctaagaca
     2221 aaaaacatca agccaagcgg tactggagaa tctccattag caaatatcga ggagtgggtc
     2281 aatgtcgttg atcctgtaac aggtaaaaaa ggccgtcgcg aaacgaatac aatgccacaa
     2341 tgggcaggaa gctcatggta ttacctgcgt tatatcgacc ctcataacaa aaaagaacta
     2401 gcagattatg aaaagttgaa gcgttggcta ccagttgaca tctacattgg tggagcagaa
     2461 catgcggtac ttcatttact atatgcacga ttctggcata aattcttata cgatatcggt
     2521 gttgttccta caaaagaacc attccaaaaa ctatacaatc aaggaatgat cttaggcgaa
     2581 aacaacgaaa aaatgtcaaa atctcgtggt aatgtagtca atcctgatga tgtggtcgaa
     2641 acttacggtg cagatacact gcgcatgtac gaaatgttca tggggccatt agatgcttcg
     2701 attgcttgga gcgaaaatgg cttagaagga agccgtaaat tcttggatcg tgtatggcgt
     2761 ttgatcgttg atgagaacaa taaaatgcgt gaccgtatca ctacattgaa tgatggcaag
     2821 cttgataaag tgtatcatca aacagtgaaa aaagtgacag aagactatga aaatcttcat
     2881 ttcaatacag caatctccca attgatggtc tttatcaatg aagcctataa agtggacgct
     2941 ctgccttatg aatatatcga aggatttgtt caactactcg caccgattgc tcctcacatg
     3001 ggtgaagaac tatgggcaat ccttggaaat gatggtggta tttcttacgc accatggcca
     3061 acatacgatg aagcagcact tgttgaagac gaagtagaag ttgttttcca agtaaacggt
     3121 aaagttcgtg cgaaaagcaa tgttccccga gacttaggaa aagatgagct agaaaaagca
     3181 gcattagcca atgaaatagt gcaagaatat attgaaggca aaacagtcag aaaagttatt
     3241 gcggtaccaa acaagttagt caatatcgtt gctaattaat tggctctttg tcaataagga
     3301 ctgatgagtt gtgcaaaatc aaatctgggc cagaatgaac ttcattctgt ctcagatttt
     3361 ttgttatttc aaattgatta attgattgat caaaaaggtt ttgattttca tgttctcaaa
     3421 cacgttcaat gtctttttct tctgaatggt cttgtaagag atctctgtaa tcatcaaatt
     3481 tttgtctatt aatcttagta ttttttgatg tataaactac ctgaaattga gagtacatac
     3541 caaattcata aattttttat tagtgggact agtgattgta tggattttga cgcataatga
     3601 atgaagtatg agaaaaggag agacaaatgg atcaagaact atttattatc gaagtattat
     3661 cagctaaaca aaaaattatc gagaaagcga aaaaaagagc gaaaagaata atgttcaagc
     3721 ttttaagctt agtatttttc tctggtttgc tttttttcgg agctgtatcg ttcctatatc
     3781 gccatatgag tgcagtagtc cgttcagctg aagggatgca aaaattaaat aatttgtatc
     3841 acttttcgtg gggagttggc ggaataacaa ttgttgtgct tattgttttt cttctactta
     3901 tttggctccg ttaccatcaa ttgtcgcgtc atttcgatca atggcttagt caaagagcac
     3961 aacagttttt acataataaa ccaacttctc aagatgatga ctatctttat tttgagaaga
     4021 cttcgacgag tcaagctttt cggatacaaa aaagtgattg ccagttattg ctgagatcac
     4081 ttgatgattt acctatctac ttaggcgaaa gcaaatcaaa attcggaatg aacacattcc
     4141 atattttttt actagaaaac gaacccagat gggaaaaaag ccagtcttcg cctggtggat
     4201 acctgacaac taagatgagt acattagtca ctggtttttt cctcattctt ttagctggaa
     4261 caatctatta tgggagatac actcagccaa aaatgtcttc tcatataaat gctgcttctc
     4321 acatgacaat gaactcaact aacgatacgc atatacaaaa gacaaatgaa gggttgttag
     4381 ctgatcctaa gcaaaaggta aatttgacaa cacaaaagat aaatgatctc aaacttgata
     4441 cagagacgca ctctctctat atgacaacag atagtggtgc taattgggta tttgtgccga
     4501 ttgattcgca atggttgcgt tttggcgaat acaccctgac aacggggctg attcctgaag
     4561 gttattggat ggacaacaca tatgatattt ccgccgattt ctcatggttt atctattctt
     4621 ctggggatga tgtacgtctg ctgacttcaa gagataatgg taaaacatgg caaacgtcta
     4681 ttatcaccca atcagttcag ccgatacgtt atcgaaaagc ggtattttat ggcaacgatg
     4741 gtctatttat ctgtacatca gccagtggga tgtctgcaga aactttgtac atttacacta
     4801 ctatggatgg cggaatcact tggataaaaa gtggaagtac aatgatcaat cagccagtac
     4861 aaaatgtcag cttcgttaca acttctttag gttttgtttc gacacgtgaa agtttgtttt
     4921 atacaaacaa cggaggacgc tcattcaaag aatcagttct gtcgattccg gaaaactatc
     4981 agacaggggg gatagatctt tttcaatcac caaatgaagt tactcaaata agcgcgaata
     5041 cgttagagac aaaattttat ttgttgaaaa aaggaccaat cgatgcaggg aaaatgtttg
     5101 cctgcctctt ccgttcaact gataacgggg aaacatggca attcgttcag caactatcgc
     5161 aagtagaaca aggagattaa agaagagaga ttgtgagcct gacgttcagc accgagtcac
     5221 aagaaacaaa ttcaaaaaac agcttctcat atttgttgaa tagtgtaact cgtgattcgg
     5281 agttgacaag agcaacaaga agcgattttg aagtatagct tctcgtattt tacaagatcg
     5341 tgtcttattg ccgataggtt gtctcttgaa caccgtttat tcgtaaaaag ggattgtgac
     5401 aagtcttttg tcactactta ccgaacgaaa ctgaaggagc tatccaaaaa gtatcattag
     5461 tattattctt tgtgtttcta gtggcaccac tagtacattt aaaaaagcta tcgatcatgg
     5521 actaatcata catgatcgat agctttttag ataatgtttg ttacgctatc tgaaattgac
     5581 tattataaag actggcataa agcgttggtg attggagtag ttcagaatgt gttccttttt
     5641 cgacgatatc gccattcttc attacaagga tcatgtctgc attttcaatc gttgacaaac
     5701 ggtgtgcaat cacaaaactt gtcctatctt ttgtcacagc ttccattgct tgctgaatca
     5761 atgcttctgt tcgtgtatct acgctagaag ttgcttcatc taaaataacg acaggaggat
     5821 tcgctagaat gactcgagca attgtcaata attgttgctg tccttgagat aacgtgccgt
     5881 tttcgctaga aatgatcgta tcatatccgt ctggcagtgt tcgaataaag tgatcacatt
     5941 gtgcgatttc agccgcttcg ataatttgtt ctcttgtggc atccattttc ccgtaggcga
     6001 tattatcagc taccgttcct tcaaacagcc aggtattttg taaaaccatc ccaaaagaac
     6061 gacgtagttc tccacgtgaa agcttggtaa tatcaagacc gtcgaattcg atctttcctt
     6121 tattcagttc ataaaaacgc atcagcagat tgactaaagt cgtttttcct gcaccagttg
     6181 gaccaacgat cgctaccgtt tgttttggct ttactgaaaa atcgacttga ttcatcaata
     6241 ttttatctga tgtgtaacca aactgtacat ttttgaaagc tatttttcct ttcggttgtt
     6301 ctaaaagtct tgcatctgga aggtctggaa tttcatctgt ttcatctaat atctcaaata
     6361 tcctatcgat tgctgccatt gcggattgga tcgagttgat cacataagaa gcggtagaaa
     6421 ttggttcaga aatctgattg atgtactgaa gataagcttg taagaagccg atcgtcacac
     6481 cacctgatag aacaagcata gcaccgaata ccgcacttat gatgaaggcg agttgattaa
     6541 tgaaacgaat ggccggatag atagcacagt tcataaattg agcacgttta aaagcgttgt
     6601 attgcttttt atttacttcg tgtatagcag cttctgcatg agcttgctga ttgaatgttt
     6661 tgaccactag attaccagct aagatttctt ccattcgatt atttaaaaca cccagttcat
     6721 actgattctg ttcagaaatc tgtttgtttt tgttcgcaat cttagttgta acaattgaac
     6781 tgccaacgat cagcaataag acgagcaaag taagcttgct ttcgatataa aacagcatca
     6841 gaccagcagc agctattgtt acgacagaag taaaaaattg attgatacct gtcaataaca
     6901 cttgagaaag ttgattgaga ccggtagttg tccgactaat gatgtcacct acttgatgat
     6961 tgtcaaaaaa cgacatcggt aatgttttga atttttttgt gatttctttt cgcaccctca
     7021 atgtgatctt ttcgcttaac gctgccattg tccgttcctg aataaatgaa gtgatgctgc
     7081 ttaagactga acagccgatc agcaataaga cagggaacaa caatgcttgt ttgatatcac
     7141 tgaaagaaag ctgtccaata cctgtttttc ttatttctga taagagatca tcgatcccga
     7201 ttcccatgat caagggcatg gctacgacta ataggttccc gaataaactg catagaaaca
     7261 atccgtaaaa atatttatgt tcaggtttga tcattttcca aaaacgtatc aaagcacgcg
     7321 tgttcaacgt ttttttcttt tccatcgtat cccttctttc tatattcctt gtgattttgc
     7381 aaactcctga taagtttggc aattagacag tagttcttca tgagtacctt gtccaacgat
     7441 ttttccttca tctaaaacaa tgatgttatc tgcttgttgg attgtactga ggcgttgagc
     7501 aacaatcacg agagttttct ccgacatgtg tttatgaagg gctgctctta atgctgcatc
     7561 tgttttatag tctaaagcgg agaaactatc atcgaataca tagatctctg ctggtttgat
     7621 caacgctcga gcaatacata tccgctgttt ctggccccct gaataattag aaccgccttg
     7681 agcaacaaag ctgtccaatc cgtcaggaag ggtgtccact agttcttcta attgagcgat
     7741 cttgatcgct ttagacaact cttctgttga ggcatattca tttcccatct gcaagttgct
     7801 taaaattgtc ccgctgaata agaaagcctt ctgagggaca taactgatgt gggagcggat
     7861 ctgttcttga gtcatttgag taagaggaat accaccaagg gttacttttc ctatcgtagg
     7921 gtcactaagt ctcaagagaa gtttcgccaa actacttttt ccagatccgg tcccgccaac
     7981 gatcgctgtt gtttttcctt taggtattgt gaaatcaata tcttctagaa ctggatcaga
     8041 gatttcagta tatttaaatg tgacatgttc aaaagcagct gcatttgtct gatcaaaggt
     8101 tggttcagct actgttttga tcgtctcttc ctcaatttcg atctctgctt gcagtatttc
     8161 ttctatccgt cgtaatgagg caaacgattt agggataatg accaatacca tagctgcgat
     8221 tatcaagtaa gcaagtgtca gcatagagta ttcgactaca gcagtcacga caccgatttg
     8281 taatgctcct tctgctacaa gatttccgcc aaaccacaaa atagaagcat aaacgattcc
     8341 catgagcaaa aaagcaattg gagtgaccca agcaaatatt cggttcactt tgatcatttg
     8401 attagcatag tcagcaaagc tcttattcgt tcgcttttct tcatatgatt gattatcaaa
     8461 ggcacggatc atattaatcc cagtaaagaa ttctctcaat gtgatggtga tccgatccat
     8521 ttttggctgg atactcaaag ataaaggtgc ccctcgtttc atcagtcgat aaacaatcca
     8581 tacataaaaa acaacagaga tcaatgtaac ccatgttagt aatagagaat aacgaaacat
     8641 catgagaatg gtgaaaacgc aaataaccgg tgctggtaag atcatttggc aaaataaaac
     8701 aatcatttgc tggacattat cgacatcatt cgtcattctt gtcaaaagag atccagaact
     8761 aaaacgactg atatccttca ttgacagctg ttggaattta cggaaatatt tctcccgcgt
     8821 ttggaatcca aacctagcgg aagccaatgc tgaaaagtaa ctagaggcaa ctgcagcgat
     8881 tgttcctaga aacgctacaa gcagcatttc aactccaatt ttttttataa ctgaggcttc
     8941 gccacttgct atacctgtgt cgatcagctt tgctactaat tttggtacac ctaatgtacc
     9001 ggcgatttct aaacagatca tcacaaaggt aaggagagcg agtcctttgt ttttctgtaa
     9061 gaatgttttt aagatagaca tattattcct ccttttttcc tgatactttt cgattctata
     9121 ccttcaagta acttgaaggt caatagaaaa aagaataaac tgaaaaagag gtgagaaaaa
     9181 atgaaaaaaa tgtatacgat tggccaagta tcggaactat ttgatttgcc aaaatcaacg
     9241 atccgctatt gggatgaaca aggattaatc cgttcaagta gacaagaaga gaatgattat
     9301 cgattgtttg atatcgatga tatttttatg ctttatgata tcgtttttta tagaaaatta
     9361 gatataccga tcaaacagat gaagaattta tatggaaaaa cattgacgga gttatatgag
     9421 acacttgatg aaacagaaag aagaatccat aaagaactgg tggtgatgaa acaaaaacaa
     9481 aaagagataa gggagcaaaa aaaacagttg aaattgatga tcgatacaaa cgaggaagaa
     9541 tttcctgtag aagagatccc ttttgactgt atgatctcaa cggagtttga agatattgtg
     9601 gaaatcaaaa aatttctacc taactattca agctttggga tgatgagtat gccagcttcc
     9661 agttcacaga cgatgtatgg attttttatc gaccctagtg aagtacattt atttactcaa
     9721 gacgtgattt gggagaaaaa agatacggct gtttatcgtc gattcttatt gaaatccgag
     9781 atgaaccatg cggaacgtaa taatatttta gaaatcagag aaagaatgta tgaaaaagga
     9841 tggaaaacag gagaagtgat cggccagtat ctattgacga atacggatga aaacaatatc
     9901 agaactgaat attatcatgc atggatagaa atgaaaaaat aggaaatatg tgtttaagat
     9961 aactcgaaaa tgatggttgt tttagttata aagaaaaatt ttctggttcg attacgggtt
    10021 taatgtgggg gattattagt cgtggataga tagaatttta gttttttaga ggaaatatgt
    10081 ctttaaaaat ggaatggttg caattcttag cgatatatac gatgtaattg tcctcttaaa
    10141 aaagtatgga aaaataattg tattgggtgt tatgtataat tcatggtgaa ctgagactaa
    10201 tgagaagaaa aattcatatt gttatgagtg agattgtatc agatgagaat gataaattaa
    10261 ataggtgcat aaaagcatta cacttaaaat acattgttgc ttacattatt acaatatgtt
    10321 aataaggcaa aaataaatag atggattgaa taaacatatt ttttaatcaa atagtcccta
    10381 ctgtgacttt cctcagctat cggtgatact accgatagca tt
//
LOCUS       DACCXO010000095        10367 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.096, whole genome shotgun sequence.
ACCESSION   DACCXO010000095 DACCXO010000000
VERSION     DACCXO010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.096"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            6..305
                     /locus_tag="IVY70_002191"
     CDS             6..305
                     /locus_tag="IVY70_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7959307.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IVY70_002192"
     CDS             378..1739
                     /locus_tag="IVY70_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP7959308.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IVY70_002193"
     CDS             1761..2021
                     /locus_tag="IVY70_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959309.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IVY70_002194"
     CDS             2035..2787
                     /locus_tag="IVY70_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP7959310.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IVY70_002195"
     CDS             complement(2881..3486)
                     /locus_tag="IVY70_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7959311.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IVY70_002196"
     CDS             complement(3539..4513)
                     /locus_tag="IVY70_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP7959312.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IVY70_002197"
     CDS             complement(4891..5670)
                     /locus_tag="IVY70_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7959313.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IVY70_002198"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IVY70_002198"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP7959314.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IVY70_002199"
     CDS             7161..8558
                     /locus_tag="IVY70_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7959315.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IVY70_002200"
     CDS             8639..9079
                     /locus_tag="IVY70_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7959316.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IVY70_002201"
     CDS             9227..9553
                     /locus_tag="IVY70_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7959317.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IVY70_002202"
     CDS             9554..10303
                     /locus_tag="IVY70_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959318.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
BASE COUNT         3288 a         1829 c         2149 g         3101 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgctt
//
LOCUS       DACCXO010000096        10233 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.097, whole genome shotgun sequence.
ACCESSION   DACCXO010000096 DACCXO010000000
VERSION     DACCXO010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.097"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(225..1673)
                     /gene="cls"
                     /locus_tag="IVY70_002203"
     CDS             complement(225..1673)
                     /gene="cls"
                     /locus_tag="IVY70_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP7959319.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEIAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYILQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1825..2718)
                     /locus_tag="IVY70_002204"
     CDS             complement(1825..2718)
                     /locus_tag="IVY70_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7959320.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(2702..3388)
                     /gene="ftsE"
                     /locus_tag="IVY70_002205"
     CDS             complement(2702..3388)
                     /gene="ftsE"
                     /locus_tag="IVY70_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP7959321.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3493..4594)
                     /gene="prfB"
                     /locus_tag="IVY70_002206"
     CDS             complement(join(3493..4530,4532..4594))
                     /gene="prfB"
                     /locus_tag="IVY70_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP7959322.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4720..7254)
                     /gene="secA"
                     /locus_tag="IVY70_002207"
     CDS             complement(4720..7254)
                     /gene="secA"
                     /locus_tag="IVY70_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP7959323.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(7476..8027)
                     /gene="raiA"
                     /locus_tag="IVY70_002208"
     CDS             complement(7476..8027)
                     /gene="raiA"
                     /locus_tag="IVY70_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP7959324.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(8155..8874)
                     /locus_tag="IVY70_002209"
     CDS             complement(8155..8874)
                     /locus_tag="IVY70_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP7959325.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            complement(8871..10184)
                     /locus_tag="IVY70_002210"
     CDS             complement(8871..10184)
                     /locus_tag="IVY70_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7959326.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
BASE COUNT         2767 a         2228 c         1788 g         3450 t
ORIGIN      
        1 tatctcatta aaagtttcta tgctatactg gctttgaaat gtgtctcttt taatagcata
       61 tattagctta tttcttgctt tccccaaaaa acaagagata ccccaaagtt aatttcgctc
      121 atgcgagacg ctttctccat ttctgacgta ctctcagaaa tggccccgtt agaaaaggac
      181 acgagatctc catctgttga tggttctcat gtcctttttg tgttttaaag gagcggcgaa
      241 atcaatcttg ctaatccttc tttcatcttg atgattattc ctcggttttc atacatttct
      301 tttgtgagat gcgtagaaat tttcgagtct tcaaaaaatg cttttcttac acgtgaagcc
      361 attcgttcat cataaatcag tgtattgacc tcaaagtcca agcgaaatga acgaacatcg
      421 atatttgctg agccaactga aacaatgcca ccatcgataa tcatcgtctt tgcatgaata
      481 aatccattct catatgtttc gatccttgct ccatactcga tcagctctgc agcgaaagaa
      541 tacgttgccc agtaaacgag aagatgatct ggtttgttag gtatttggat atgaactttt
      601 acgcctgaaa gaagagccaa cttcagtgct tcatgaatcg atccatccgg tatgtaataa
      661 ggcgtttgga tcaatatctc ccgtttggct aacgaaatca ttttcaaata agtcattttg
      721 atttgttcat gttcggtatc tggtccgctt gtcacgattt gaacagctat atcaccagta
      781 gagtgaatgg acggaaaaaa tttgggagaa tagcctactt cttttgtatg ctgagaattc
      841 cagtccatca aaaaacggtt ttgcaagata tacaccgctt ctccgtaaat acgcaaatga
      901 ttgtctcgcc aataaccaaa tttctttacc aatcctagat attcatttcc aacattgaac
      961 ccacctgtat atccgatttt cccatcaata actacgattt tacgatgatt gcgataattg
     1021 atccgaggat taatatacgg aacaaataga ggaaagaaaa atgcgatttc tccgcctgct
     1081 tttttcaaat tctgaaaaaa gctggaagaa acttgtgtgg aaccccatgc atcaagaagg
     1141 actctgactt ttacccctcg tcttgccgcc tctgtcaatg catctcgaac ttcttcacct
     1201 aatgcatcac tgcgataaat ataatactgg aagtggatat ggtcttctgc ttgcttgata
     1261 tcttcaatca gcgcatcgaa tttttcacga ccatctgtaa ataaagtgat ttcattttta
     1321 gtcgtataga gcgtactttc aaataccgtg agcatataga tcaattgttt cacatctact
     1381 tgtccagtag gcggatgagg ataaagccct cgcatcaatg cctgtttgtc ttcttcaatt
     1441 tccaggttct taccgatttt cccttggatc ttcaaatcaa agattttctc tttggaaatc
     1501 cctcttccta aaaaaatata taaaataaat cctacaactg gaatgaacat caaaacgagc
     1561 agccatgccc atgtttgagc agtttccttt ctttctcgaa aaacgatgat aaaggaaagc
     1621 aaaatattca taatcaaaag gatcgtaaaa aaattgtcaa cgataatttt cacggcttca
     1681 cctcttcctc taccaactta agaataccca agttgttttt taaagtaaag ggaaaaaagc
     1741 cgcttccttc tattctccta gccaataaaa aaagaaagct ggaacgaaac tgtccaactt
     1801 tctcatttta tttatattgt ggtattagaa tttcaagaaa cgtctcatag aaatgatcga
     1861 gccaagtgaa ccaatgatca caccaatgcc gaccattaaa agattgattt tccaaacgat
     1921 attttgtgga tgaatcaatg aataatggga acgtaataat gatggattga tcaattggaa
     1981 aaaccatgca taaccaaatg tgatgatcaa aatcggaaga atcgcaccga tcagaccgat
     2041 ccagccacct tcaaggaaaa acggccagcg aatatatcca ttttttgcac cgactaaacg
     2101 catgatttga atttcacgct gtctagacaa aatcgtgatc cgaatcgtgt tcgaaatgag
     2161 aaacatcgct acgaacagca gcaatgccgc tgcaactaat ccccatgtcc gaactgcacc
     2221 agcgatttgg aaaatccgat ctgaggaagc tccaccgtaa tcagaacgag aaacattctt
     2281 caaatctgct gcttcttctg ctacttgttt tgtataagaa ggatcagtag cacttactac
     2341 atacacatca tataacgggt tattgtcacc ttcaaacaat ttccaggaat cacccatttc
     2401 gttttggatt tttttcaatt cctgttcttt acttgaaaat tcaacttttt taacatgatc
     2461 taagtcattc aattcttttt ctagcttttt catctcttcg tcttttgtac cgatatctac
     2521 gaatacagat acatcaacgt ttccttctat atcctgagct aatttagttg cattcatgat
     2581 aactgccatg aatacaccga ctaaagtcaa cgtgatcgtt accgcactga ctgaagcaac
     2641 tgtcatccag ccgttacgtt tcaaactctt gatactttct aataaatggc ggaaaaaggt
     2701 tctaatcatc gtatccatat tccccttccg cttgatctcg aataatgcgg ccgttttcga
     2761 tagcaattac tcggtgccga atcgtattta cgatcgtact gttatgtgta gccatcacga
     2821 cagtagttcc ttgggcattg atccgatcta ataatttcat gatttcccat gagttttctg
     2881 gatccagatt tcctgtcggt tcatccgcga taagtacttt aggcgtattc acgattgccc
     2941 gagcaataga gacacgttgc tgttccccac cggataattc gctaggaaag actcgaactt
     3001 tgtgtttcaa accaacaagg tccagcactt ccattactcg ttttttgata tctcgaggtt
     3061 ttctgccgat aacttgcata gcgtacgcaa cattttcata tactgttttt cttggtaata
     3121 atttatagtc ttggaacacc acgccgattt ctctgcgtag ataaggaact tcgcggtttt
     3181 tgattgctat tagatcatga cctgcgacgt tcaaagttcc ttttgaagct ttttcttctc
     3241 gatacatcaa tttgatgaat gtagatttac cggctccaga cggaccaacc acatagacaa
     3301 actctccttg ttcgatgaca atcgagagat tgcggatagc ggtcgtacca tttgagtact
     3361 tcttcattac atccttcatt tcaatcatgg cgttctctcc atatcttttt cagtctttag
     3421 ctattatagc atggcaatgt ttcaacatgc ttgcaaaatc ttacaatttt atttcaattt
     3481 aacaatgggt tcttattgta gatgcatttt taaatagccg tcaataaata aatcaaggtc
     3541 accgtccata actgcttgga catttcctgt ctctacgttt gtccgatggt cttttaccat
     3601 agaataggga tggaaaacat aagaacggat ctgtgaaccc cagccgattt ctttttgttc
     3661 tcctcgaagt gcagctgctt cttgttcttt cttttccact tctaattgat aaagcttagc
     3721 ttttaacatc cccatcgctt gctcacggtt tttcaattgt gagcgttgtg cctgactagc
     3781 tactactgtt cctgtaggca agtgagtgat ccgtacagca gattctgttt tattgatgtg
     3841 ctgaccacct gcaccacttg ctcggtacgt atcgattttc aaatcatctg gattgatttc
     3901 tatatcgatc gtttcatcaa gctctggcat cacatctacc gaacaaaaag aagtatgacg
     3961 tcgtttcgct gagtcaaaag gcgaaatccg tacaaggcga tgaacacctt tttctgattt
     4021 tagataacca aaagcgttat gtcctttaat cagcagggtc acactcttga tccctgcttc
     4081 atcacctgct tgataatcta gtgtttctac ttgatagcca tgctgctctg cccaacgtgt
     4141 atacatacgt aaaagcatgc tgccccagtc ttgggactcc gttccgccag cccctggatg
     4201 caattcgacg atcgcattgt ttcgatcata aggtccgtct aacagcatag ataattcata
     4261 cgtcttcatt ttttcatcta atgcattgat acgtgtttct gcttcttctt gcattgctgc
     4321 atcaggttct tcttgcagca tctccaccat caattcgatt tcttctagtt cttcggcaag
     4381 agaatggaat tggtcatagg tttctttatt agcgttattt tcactgatca ctttttgtgc
     4441 ggcttcgctg tcatcccaaa aaccaggttc agccattttt gcttctgctt ccgcgatgtc
     4501 ttcttccagc tgatctaagt caaagagacc tcctgaaact tgtgaccttt tcctgcattt
     4561 ctgtcaggag atttcgaatt tcggatattt ccatgttttt tccttctttc actatacttt
     4621 tttcattata tctagtctaa cagaatcagc aaataacaga aagaaggtga gacgaattct
     4681 taaaaaaaga atttgtctca ccaatcctgc tgtcagaaat tagtttgcat tttttccatg
     4741 acagttctta tattttttcc cgcttccaca aggacaagga tcatttcttc cgaccttgtt
     4801 cacatggatt ggctgttttt tcgcgctagt attactattt tctttggcat ctccgtcttc
     4861 tgagtgagaa gctgttcctt gagaaacctg ttcacgctgt acgttttgac ggatctcagc
     4921 tttcataaag attcgagtga cttcatactc gatggcgcct accatatctt caaacatctt
     4981 atagccttct gtttgatatt cgactaatgg attgttctgt ccataagcac gtaagccgat
     5041 tgactgacgt aattgatcca ttgcgtcaat atggtctgtc catttagagt ccacaacacg
     5101 caagatcacg actttttcaa attccaacag ttgctctgga ccatttagtt gagccgcttt
     5161 ggtattaaat acttcttgtg ctcgttcgtt tagatactct ttgatctcct gagctgtttt
     5221 tccttcgata tctgcaagag taatactatc ttcatggaca agagtactgc cggcaaaatc
     5281 gatgatactt tccaaattcc aattttcttt ctctaattgt gtatgcgcat ctactacacg
     5341 actgattgtc cgtttgacca tgttcattag tggcttggat aagctttttt cttccatgat
     5401 tacttcttgt cgctgtttat agattacttc acgttgttca cgcatcacat catcgtattg
     5461 taagacgttt ttacgtgtgt catagttatt tccttctaca cgtttttgtg cagattctac
     5521 ttgtttcgtc agcatcttgc tttggataac agcatcttcc tcaccgatct tcatccgatc
     5581 taagaaagct ttgattcgtt ctgaaccgaa tcgtttcatc aaatcatctt ccaatgacaa
     5641 gtagaactgt gacataccag ggtctccttg acgtcctgca cgtcctcgaa gctggttatc
     5701 gatacgacga gattcatgtc gttctgtccc gataactgct agaccaccaa gttctcttac
     5761 cccaagtcct aacttgatat ctgttccccg accagccatg ttggtagcaa tcgtcacagc
     5821 acctttttga ccagcattca tgatgatctc tgcttcttta aagtgatttt tcgcattcag
     5881 cacttcatga ggaatacgtt cttgattcag catatctgat aataactcag atgtttcaac
     5941 cgcaacagtt ccgactaaca caggttgacc tttgcggtag cgatctttga tatcttgcac
     6001 gacagcgtgg aacttgcttt gtaatgttgg gtaaagcaaa tctggacgat catcacggat
     6061 aaccggacga tttgtcggaa tctggtatac ttggatgtta taaatttctc tgaattcttc
     6121 ttcttctgtt ttagctgtac cagtcattcc cgcaagtttc ttgtacatac ggaaatagtt
     6181 ctggaaagtg atcgtcgcca tggttttcgt ttcgtcttcg atctctacgc cttctttagc
     6241 ttcgattgct tggtgaagtc catctgaata acggcgtcca tccatgatcc gaccagtgaa
     6301 ctggtcaaca atcagtactt ttccgtcttg gacaacataa tcgatatcta aaagcatgat
     6361 atagttggct ctcaatgctt gatccaagtg atgggtcaat gcagtatttt caatgtcgta
     6421 taagttatcc aaaccgaatg tttcttctgc tttttcgatc cctgcttcgg ttaaactgat
     6481 tgtttttgat tgtacatcga ttttataatc ttcttcttct ttcaaacgtt tcacgaagtt
     6541 gtctgctctt gtatataaag ctgtggattt ttctgcttgc cctgaaatga tcagcggcgt
     6601 tctcgcttca tcaatcaaga ttgagtcaac ttcatcgaca atggcataat tcagcggacg
     6661 ttgtaccatt tgatggcggt aaacgaccat attgtctctc aagtagtcaa atcctaattc
     6721 attattcgta ctataagtga tatcacaatt ataagcctcg cgtttttcat ccgaagattt
     6781 tgagttgata ttcaacccca ctgttaatcc taagaaatta taaagttcgc ccatttccgt
     6841 cgagtcacgg gttgctaaat attcattgac ggtaactacg tgtacccctt cgccagtcag
     6901 agcatttaga tatactggca tcgttgcggt caaggtttta ccttcaccag tacgcatttc
     6961 agggatattt ccatcgtgaa gcacgattcc acccatcaat tgtacgtggt atggatataa
     7021 acctaataca cgttttgctg cttctcggac gactgcaaaa gcttccggaa gtaattgatc
     7081 taatgtttct cctttttggt atctgccctt gaattcagca gtttttgcct gtaattgttc
     7141 atcagttaat gctgccattt ggtcagcgta tgcttcaact ttttttgcaa tgctatctaa
     7201 acgttttagt tctttcttat cgttttcgat aagtgaacga atcaaattgg ccatgcgttg
     7261 tgtaatctcc ttttccttct gtgtattatc cagactatgt agactttact tttcatttta
     7321 tcattagtta tagataattg aaatatctta agcgtattaa agtaagaact tcataattta
     7381 ttcatatttc atctaaaaga ggaggaaact caaaaaaact tgcggcagct gtcagtagct
     7441 gctgcaagtt ttcgttgtta tttaagtaag gctgatcagt ctgtttcaat cagtccgtat
     7501 tttccatctt tacggcggta aacgatactt gtaccatttg tttctgcatc ttcaaaaata
     7561 aagaagttat gtcccagcat gttcatttgc aagactgctt cttcactatc cattggtttc
     7621 aatgaaaggc gtttcgtacg tacgatatct aattcagaat cattttcgtt ttcgtcctct
     7681 tcattgaata aagcagcagc atttgcagta ttcatgcccg tttcacggga tttacgatta
     7741 atttttgttt tgaatttgcg gatttggcgt tccaatttgt caacaaccaa atcgatactt
     7801 gcgtataaat ctggagaagt ttcttcggca cgtaatacaa gataaggtaa tgggatcgtt
     7861 acttccactt ttgctgtttt ttctgtataa acttttaagt tcacatgcgc cgttgcatcg
     7921 ggtgcgtcac tgaaatagcg ttctagtttg cctacttttt tctcaacgta gtcgcgaatc
     7981 gcttcagtta cttcgatatt ttcgccgcgt acattatatc taaacataaa aattacccct
     8041 ttcgtctacc acaatcaaga aggagaagga ccactctttc cctcttctta attctattat
     8101 atcgaactct aggttatttg caaattaaat cgctatcaaa tttccacatc taattcatct
     8161 cgccaaagaa atagttcgga tgattttcgg ctgtgcgatc tgcaaacaat ctgctgcgtg
     8221 gaaaagagtc tgtcctgttg tatagacatc atcaataatc aatatttttt tattttgtat
     8281 cttagaaaag gtgactgcaa gttcaaaagg ttgaggcatc tccaatcttt cctttcgcgt
     8341 gttttttgct tgagccagca gatgcttctc tcttttgagt agcatctgaa aaggaattct
     8401 tgctgctttc aacatctcgc ttacttgatt gaatcctctt tctgttcgcc ttttttcaga
     8461 taaaggtatt ggacaataga tgtatgtcgg gtattctttt tttatctgcg tccatttata
     8521 tgcaaaggtg cctgctagtc ggatatctcc cataaacttg tattgattga gccaggcctt
     8581 catattttca tcgtacacaa aaaaggcacg gtgctgaaaa tcataggtcg gatatttctt
     8641 cttccattct tgacagtcac tgcagtaaac ggggatagaa tttactttct ttttcgtact
     8701 ctctagattg aaatggattg gtttttgaca gccagaacag cctcttgttt ccaataaatg
     8761 aaattgtgcg cgacaaactg aacaagtctg aggtaatctg tatttcgaaa caccaataat
     8821 ctcgctgaat gttaaatttt ggacgatatc tttactgcag caactgcacc tcacagtatc
     8881 ttcttccctt cttcattcat ttctttgatt tcctgacaag cttttcgaat agctgcagtt
     8941 ttttcttcat agaaaaaata tacttgactg ttcgtataag gccctttacg atcggctctt
     9001 cctgcgattt gtaccaaaga agatttagta aaaaccggat gatccgctcc taaaacgata
     9061 acggatattc tttcaaatgt gactccacgt tcaaggatca tcgtggttaa cagtatgtca
     9121 tattttgctt ctcgcatttt ctggactttt tccgcccgct gattgtctct tgaagaaaca
     9181 tctgtgatct catatctcgg aagagcctct tgaagtgtgg ctttcaaacg atgcatcatc
     9241 gtgatacttg gacaaaataa aaggatatgg ttttctttaa ttaattgatg aatgatccgc
     9301 accaaagatt ttatcttctt cttgttcttg catttttgtc gccaattatt catgaaaacc
     9361 aagacgggtt caggaagcaa acgccgatga aagcgtaaag caagtttgtg gatgtcaaac
     9421 gtctgcttga tctcctttag taacttttta tcaggtgtgg cacttaagta gatgaatcgt
     9481 ccgtctggtt taatcgctgt ttgcacagca tgagctaatc ctgcatcccc gttataagga
     9541 aacgcatcga tctcatcgat cactattaaa tcaaaggacc gataaaaacg gtatagctga
     9601 tgaatcgtac agataacaaa agaagtaaag gtataagccg ttgctgtatt cccatggagc
     9661 aacgtgattt tttcttctgg aaaaacataa cgatagcgta aaaacaattc attacaaaca
     9721 tcgactcgcg gtgtacaaat cgctactctt cctccagctt ttaaagtttc cactaaaata
     9781 ggataaagca tctctgtttt ccctgcacct gttacagccc aaataagatg atgtcttttt
     9841 tctgaatggc aaagtattct ctttgagatt tccgtttgtt tttcagtaag ctgtcctgtc
     9901 caaaaaaaga cgacttctcg aggttttgct tcaggctgat caaacaaata taaatcttgt
     9961 tgagtgtcgc atcgccctaa atatagacaa tgtgtacaaa aataaccgta atctgcaagc
    10021 tgtccatcaa tgacggacgt tctcattcca cagcgatgac aaagaatcca gttgctcaaa
    10081 cgttccactg ctgcgatctt ctcataattt ggtaaattta cctcctcaca gtctattgct
    10141 ttaatatctc tcgtcagaag ccttctaccc caaaaatgat tcatattatt cgccctaaaa
    10201 aaatatacga aaaaacaagc gatctatttt ttg
//
LOCUS       DACCXO010000097         9921 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.098, whole genome shotgun sequence.
ACCESSION   DACCXO010000097 DACCXO010000000
VERSION     DACCXO010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.098"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            177..1049
                     /locus_tag="IVY70_002211"
     CDS             177..1049
                     /locus_tag="IVY70_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7959327.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            1046..3004
                     /locus_tag="IVY70_002212"
     CDS             1046..3004
                     /locus_tag="IVY70_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7959328.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(3336..4187)
                     /locus_tag="IVY70_002213"
     CDS             complement(3336..4187)
                     /locus_tag="IVY70_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7959329.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            4310..4777
                     /locus_tag="IVY70_002214"
     CDS             4310..4777
                     /locus_tag="IVY70_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP7959330.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(5040..5522)
                     /locus_tag="IVY70_002215"
     CDS             complement(5040..5522)
                     /locus_tag="IVY70_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7959331.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(5622..6485)
                     /locus_tag="IVY70_002216"
     CDS             complement(5622..6485)
                     /locus_tag="IVY70_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7959332.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            7083..7958
                     /locus_tag="IVY70_002217"
     CDS             7083..7958
                     /locus_tag="IVY70_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959333.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(8103..8255)
                     /locus_tag="IVY70_002218"
                     /pseudo
     CDS             complement(8103..8255)
                     /locus_tag="IVY70_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(8523..9854)
                     /locus_tag="IVY70_002219"
     CDS             complement(8523..9854)
                     /locus_tag="IVY70_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7959334.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT         3125 a         1972 c         1718 g         3106 t
ORIGIN      
        1 ctatataaaa ttgattacgt taacaataat tcgtttcatg tgactagatc atactaggca
       61 tgaaaaatct ctgaggaaca tggtttccct aggaggtttt tcgtgtctat ttttgtttgg
      121 ccgcaatcaa ctggtctagc aaacgtgaaa accaaatctt caaaaagggt gcgcgcatga
      181 aaatcaaaaa gatctttaat caaaatgcag tattggtaga tgacggtgga caggaaaaag
      241 tagctattgg aaaagggatt ggatttgaca gaaaaagaaa tgatttgatt tttgatcgtg
      301 atgtcgaacg gatatttgtc atggaaccag aaggtcaaat caagttgcaa aatttactta
      361 atcagattga tgaacgcttt ttatttgcag cagaaacgat cattgatcat gcggaaactg
      421 ttttgatgga aaagttgaat gaacatgtat tgatcgcttt gaccgaccac attgcttttt
      481 ctgcggagaa cattaataac ggtatcgtca tccgcaacaa acttttaaga gaaatcgaag
      541 tgctttacag tgatgaattc agtattgctc aatgggcagt agagtatctg acaaaagagc
      601 ttgggattcc atatacttat gacgaagcag gatatatcgc aatccatatt cacagcggac
      661 gaagcggtcg gaacaataac catcgcagta tccgtgaagt gacgattgta tctgacatca
      721 tccatttagt ggaatcagaa ttggaaacag atatccattc agaaccattt tcactgaatt
      781 attcaagact ggtgaatcat ttacgactgc ttctacaacg aacccatgcg caacaatatg
      841 ctgttttaga cacggaaatc gtagaaatgg tcaaacgaaa atacccagaa agctataaga
      901 tttctaaaaa aatccgtgtg ttgctgacca aagaatacca gctcgcgatc acgaaagaag
      961 aactgggtta cttggccatc catatcgagc gtttaagaag cgcaatcgta caaacaaatg
     1021 ttaacaatca tgaggaggaa aaaaattgaa agcatatcta caacgaatgg gacgttccct
     1081 tcaacttcca gttgcggtct tacctgctgc tgctttacta gtagggatcg gaaactggtg
     1141 ggcatcttat agtaacgaca ttgtcgctca ttttttacaa gctggcggta acgcagtttt
     1201 aggtcaatta cctttgctgt ttgccgttgg gttagcacta gggatgtcag tggacaaatc
     1261 aggtgctgct gccttggcag gtttggtcgc atttgaagtt ccagtaaatg ttttaaaaac
     1321 tgattcagta gctactttat tgaatatcaa accagaatca gttgatccat cttttgctca
     1381 gatcagtaac gtatttattg gtattattgc cggattgatt gcggcagctc tttataatcg
     1441 tttccacgaa gcaaaattac cgatggcttt atctttcttt agcggcaagc gtctagtgcc
     1501 aatcttggct gcctttgtca tgttgctgat cagtgcagtg ctactgctcg tatggcctcc
     1561 ggtttactct gctttagttt cttttgctaa attcattttg agtttaggct ggggtggtgc
     1621 aggtctttat ggcttcttca accgtttact gatccctaca ggattgcatc aagcaatgaa
     1681 ctccgtgttc tggtttgacg tagcaggaat caatgatatt ggtaaatttt tggcttctga
     1741 aggaacaaaa ggcgtaacag gaatgtatca agctggattt ttcccaatca tgatgttcgg
     1801 gcttccagca ggagcatatg ctatttacag aaatgcacgt ccagaaaaca aagcaaaaac
     1861 agcttccttg atgttagcag cagcttttgc ttcattcttt actggtgtaa cagaaccact
     1921 tgaattctca tttatgttcg ttgcttggcc attatatgtc ttgcatgcaa ttttcactgg
     1981 gatttcttta gcagtgagtg cattcttcca ttggacagca ggatttgcct ttagtgccgg
     2041 atttgtcgat tacttcctat ctttgaaaaa tccgattgcg aatcagccat tgatgttgat
     2101 cgtccaagga ttagtaacag cagtgattta ctactttggc tttaactttg ctatcaaaaa
     2161 attccatctg atgactcctg gccgcgagga agcagatctt tcagatgacg atactgcaac
     2221 gactaattca gacaataaat atgcggcaca agcaagcaaa atctacgctg cattaggcgg
     2281 agccgacaat gtaacttcga tcgataactg tactacacgc cttcgtttgc aagtcaaaga
     2341 tacaggcctg atcgatcaaa ataaaatcaa agccactggc gtacctggta tgaagatcat
     2401 cgacggaaaa aatgcgcaag tcatcgtagg tacagaagta caattcgttg cagatgagat
     2461 ggcaaaacta catggtggtg ctgcaacacg acctgcacaa acaaacaccg tggttaaaac
     2521 agaaacattt gccggtggaa cgatcgaaca agatatttac gctatcgcaa atgggaaact
     2581 gattccaatc actgaagttt cagatgatgt attcgcggaa aaaatgatgg gtgacggcta
     2641 tgctgtatta ccagaaaatg gagaaatctt ctctccaatc gctggtacga tcacaaacat
     2701 tttcccaacg aaacatgctg tcggaatcca aacagatgct ggtatcgaag tcttgcttca
     2761 tatgggcatc aacactgttg acttaaaagg cgaaccattc actttatatg taaaagaagg
     2821 tcaaaaagtc gcaagaggtc aattgattgc tttagttgat cttgccgcta ttcaatctgc
     2881 aggcaaaaac acggatatgg tcgttgtatt cacaaatggc gacaaagtcc aaagcttaga
     2941 aatcgaaccc gcacgtgatg taaaagcaaa tgataaaatc ggctctgttt ccaacaaagc
     3001 ctgagccatc gtcttcccca gactttttta tcgataaaca aacaggagct gagacaaaaa
     3061 tcttgtctca gctccttttt ccgaataaaa acggcgttct cctgccaact ccgcggtaat
     3121 aagtccagcc aaacgtaaca tatgaagagc tattttttat ttgactcttc ttattacttg
     3181 atgcagaaca gcttttttac aacctctgat aaacggtgtt caaaaaccag tttatcggta
     3241 tgaagtcaaa tttatgcaaa aatgtggaaa gccattttca caaaattttt cttcatactc
     3301 aaaactaatc ggctttttca caacctcttt atacattatt cctgatccaa tatttctttt
     3361 aatgctgctt caagagaagt taatggtttt cccaatactt tttctaaatc atcagaggaa
     3421 atagctaact gatccttttt aatatcatgt tggatcgcta aaaacatgtc agctgcttct
     3481 tgagggaatc cggcttcttt tagattttcg acaaacgctg catcacttgc ttcaataatt
     3541 tctaactttt tacctgtcgc acgctctaat gccttggcta attcttcata gtcagttaga
     3601 ttgccggata attctaagat ttctggagag tcactttcta ctagtacttt tgctgcagct
     3661 tctgcatatt cacgtttcaa tgcccaacct gtttttccat ttcctgcagc ataaacgaat
     3721 tgaccagttt ttaaagcctg cccaatgata ggtaattcat tctctacata ccaattattc
     3781 cgtaaaaatg tatgggaaat acctgaatcc ttaatcattt tttcagtgaa ttggtgatct
     3841 ggcgctaata cactgatcga gtgatcggca tctgcaaaac ttgtataagc gatataagat
     3901 accccagctg cttgtgctgc cttcactaca ttcttatgtt cggcttgacg atttcctgga
     3961 acgccagaga caaaaagcaa tcgatctatt cctttcaacg cttctttcat tgagtctaaa
     4021 tcatcataat caccaatacg tatattaaat ccagcttctt tcagtgcttc tcctttttct
     4081 tcactacgta ctagtacata gatttcattt tgtggaacca tcttttttaa gaattccagc
     4141 gcataaccac caaaacctcc agttgctcct gttactaaat aatgcatatt tgccactccc
     4201 tattcttttt atttgttaag atttacagct gtacatttat aaattacacc tttatcataa
     4261 agagtgtggt aagataagtc aagcaaaaaa aatgaaagga tgacaactga tgaagtattc
     4321 tctgcaattt agtgatgcaa tccatgtact tgcctatatt gaaatgtttc aagatacccg
     4381 atcactttct agtgagatga ttgccagtag tatcgaaaca aatgcagcaa atgtacgtaa
     4441 aatcatgagt cagctcaaaa aatccggttt gatctttaca aaaaccggaa aaccgcagcc
     4501 ttcccttgcc aaaagtccag atcagatttc tttactagat gtctatcgca gcatcgaagg
     4561 taatacacag cttatccaag tcgatccaaa aacaaatcca aattgcgtcg ttggtgcgaa
     4621 tatccaagaa gctttagaag aaagctacgc tcggctgcag aaaaaggcag aagaagaaat
     4681 ggctaagatc acacttgata gtctgataca taaaatagcc gttttggaaa aagaaaaaag
     4741 accagaaaac acaggcctcg tagagagatt tttataattc tctctactgt gcctgcattt
     4801 ttctgatcgg aatcgtctac ataaattctt ttattctccg ggggacaaaa gccttgtcat
     4861 gccccttttt cctctctaat gctcaggtca aagcgctgaa gtcatgacgt cttgataaac
     4921 ggtgttcaag tagctgaccc tcggcattag ttctgaaaaa tgcaaaatat ggaaagctat
     4981 ttcccatttt tcctctctaa tgctcaggtc aaagcgctac tttcacgacc tctatattat
     5041 catggtttat tgactaccgg tatccagatt tctcccatat atttcccgtt ggtaactccc
     5101 atttcgacta cagcatttgg acccccaaca taggcgtaat cttctacttc tcttaaaaca
     5161 tgcccaaagg ccgtattcgt caaatcattt cttaattctt caggcgattc tgctaaagcg
     5221 attacaacaa gatacgttcc ttttgggaat gggatgattc gggtagtgtt tggaacatcc
     5281 tgactcgttt gaacaccgac atagtacatc attttcccgt tatagggttc gttgactgta
     5341 aagaaataat gattttcagc agcttgtttc aatgcatcta ttgttccatt accgataact
     5401 ttttgctcaa atgcttgttt ctctgcagcc aaaccgacaa aatccgtatt gctactcttc
     5461 aattccacac caatacccgt caccgtaaaa tctggttttt cttcgatcct atactttgcc
     5521 atttctccca ccctttctat tatattaagt tcatcttaca taaaattaaa gggaaatgac
     5581 acgattacgc tcttttttct agggatattt ttatttttta actatttttt tgctttcact
     5641 gcaatccaaa tctcactgta aacattactg aaatcctctc cccattttgt aaaagagatc
     5701 tcgggagctt cgactaactc ataatctgaa gaaggaagcc attctgcata gattttcccc
     5761 caagtatctt gtaaagtttg gggaaatggc cctttattag gaaaaatggc ccaagtgtgt
     5821 tcttctatag caagcatttc taaatcagcg tacggatttt cttttgtcgt agcaaagcca
     5881 atcatgtgca tcaaactgcc cttttcttcc aagcgggctc catcaaatcc ataagaaacg
     5941 ttgattactt gatttggata taaatctcct aacttatgca tctcttccct ttgagtctcc
     6001 gtgatcgtct gcgctaactt ttgtatttct tgattgactc cttcaaattt gacaggaact
     6061 tttttcgcca cacctgccac ataaaactgt tctttctttt cgatacgaaa ttccatcgat
     6121 tttcctcctc tgacatcaat aaaaaatgtc aagcgtggaa aagatttttg tatgccgtat
     6181 ctgctgattt ctgaaggcag atatcctgac caatcacgaa aagctcttga aaaaccttcg
     6241 actgtttcat atccatatct aaacgccgtt tctgtcactc ttgatccatc taggagatcg
     6301 cgattagcca aagccaatct acggtttttg atatagtcca ttagcgacat ccctgcaata
     6361 aacgtaaaga tgcgttttaa ttgatattca gagattcctg tattttcggc tattatttta
     6421 taggaaagtc cttctgtcaa atgttcttcg atgtaatcga tcaattggtt cagatcttta
     6481 agcatattta tcttcctcct tgcttgcctt tttatcatat tatcaaagaa agtcaattac
     6541 ttgatgaatc aggaaccaat ttagtcagat gtagaaaatg ttgtatggat ctaaagaaaa
     6601 ttctcaagca gcgtttcgta acggataatt ggtattatct gcctatctat gttttacctt
     6661 tagaaaaata aattttgggg aagacacatt ctaagtaaac aaagcggtaa aaatatcttt
     6721 tattccctta gttaatccgc ttactaccta ctgtacaaag caaattgttg ttcaaactgc
     6781 tgatcagaac gctgcttgga tttttctttg cgatacatca tacctctttt atcaattgga
     6841 acaattattt atttctaaca aaatcacttc atttttgtca gataaataaa aaacaaaaaa
     6901 agttccatct ttaaaacacg ctattctttt actttttctc acattctcta agaaaagaaa
     6961 aggagaatga atttttgaca attcgagcaa aagtgattat caaatatgta aaatcagcta
     7021 cttttttctt aaacgtttct ttatactaaa tataaataag tgtttcatga aaggagaact
     7081 agatgaataa ttggcttgag tattttcctg aaaacgtttt ggaacgcggt tacagctatc
     7141 atctgcacgg ttttgtccgt catttgaact atacaagcaa gtacttgtcc gcaacagttt
     7201 ctggcacgga agattataag gtagtgatca cttgggatga aaagaccaac atgacttgtg
     7261 attgtctata tgccatagaa ggaaaaaaat gcaaacacat ggctgctgtc ttattcgctt
     7321 acgaagaacg tccaataaaa aaatccaact actcactttc cgaactctcg tcccttgttt
     7381 cctccgctag ttcctctctt gtacgggagc ttttaacaga gattcttata gaacatccgc
     7441 aatttatcga acgcttcaaa gttaagatgc catttcatgc aataaattat tcagataaat
     7501 taacaactat catccataaa tacgatcata ttatcaagaa aaacaaaaac aggaaaacag
     7561 cgaagttcat catggagatg cgaaagttca tccaagaagc agttgaatcg ctcattcagc
     7621 aaaatgccta tcttcccgca ttcgaattga tcaatgaagt gattgctact ctcgaaacat
     7681 tctattggga gcctgaggat gaacgaaccc ttcttctgat agaggactgt tattatttat
     7741 ggaaagaatt attagcagaa gcacctcatg cagaaaaaag gcaaatgttc tcttggttcg
     7801 tctgccaagt cgatcatact gacgcctctt attcaaaacg ctactccata aaaatcctga
     7861 aagaagactt tcgggaaaaa gaattttcta accaaaagaa aaaaatagat aaaaaaacta
     7921 aagaaacggt aaagaaagac gatttcaatg aaaaatagca agtaaacgat aacaaccatc
     7981 ctaaatccac aagaaataca gcagaccttg ctttaaaaac acgaatcatg caaaggatga
     8041 agtaaagaag acttaacgcg tacccaatat tagtcatttg actattaggt acgcgttttg
     8101 ttttataagt catgacaaat ttccttcgtt gctgggtaga ttttccggca ttgttgatag
     8161 attttatgat acttttctac attttgtaag agaggttcca ctgcatctgt atattggata
     8221 aaattctgac aacaactttc aggtgtgtcg aaccactcta atctttaaag ataaatatta
     8281 aaataacgat taataatatt aacagtcttt atttttcacc accaaaaacc tatctttgca
     8341 tatactaaat agacgtatat acacagatag gtttgtcttg tgaaaaataa tgattatcct
     8401 taccaaaaaa tgtttgtttt tctacatgtt cgtacttatc aaaaaatatg atccttattg
     8461 aaattactgg ctatttatat tccaacgact tcttgtgtta atttttgtta tctcatagta
     8521 aattatgttt cattttcact taatttagtt gtcacgtgtt cgtcaattcg taaaaacggt
     8581 aaatatataa acacacttgc ggctataatt aatatttgta aaacagctgc tcgccagtct
     8641 ccggctgttg ctaaaaatcc cgatataact ggtggcgtag tccaaggaat ttgcactact
     8701 acatgattaa ttaatcccac agcagttgca aaatatgcta ctgtcaaaga aaaaatcggt
     8761 gcaagcacaa agggaataat cagactgata ttaaatacaa taggcaatcc aaaaataata
     8821 ggttcgctta tattgaatat agcgggagtt accgataatt tagcaaagtc cttataatct
     8881 tttctacgac tgaagataaa tattgcaatc aataaagcaa tcgtatttcc cgatccaccc
     8941 attaccgcaa aggtatcttt aaaggccata ttgataatat gtggaatttg ctctttgttt
     9001 gcataagctg ccatattttc ttgcatattc tgaatcaaaa acggatctaa caatgaacct
     9061 gatattactg cttgatggat accaaatcca aaaaatagat tagcaataga tgtaattaac
     9121 aaaaatcctg gtaaaccagt tgtaatttta cttaatggct ctgtaactat cgtggtaatt
     9181 agcttattta tatccatatt aaacaattga atgattataa atgacaataa agcaaacgag
     9241 gtaatcgtaa taatgattgg gattaataca ttaaaagact ttaaaacagc gggaggaata
     9301 tttccggata gctgaatctg tagtttctta ttattagaaa gtttaataaa taattctgta
     9361 gccgctaatc caacaaatat acctacaaac attcctgaag ctcctgtata ggatataggt
     9421 atcacacccg tcacttcaac agctttatcc gcattttcag gagtaaacat catctgcata
     9481 ggtgttaata caacaaatgt cgaaatagca actaataccg ctgcaatatg atttgtataa
     9541 tttctgcttg cacacatatg ataagatact gctgctacaa ctaataatgt agttatgctt
     9601 aatgtaccat tggcaattga agtaccaatt tgttgtaacg tggtcaatgt actagtatcg
     9661 acaatttccc ccatgaatcc tgttggttct aagatcactg aattaataag tgtcacaaat
     9721 cctgctaaca tcataaatgg cataaatgta gcaaacgcat ctcgcaaagt tcttaaatgg
     9781 atctgattcc ccaacttagt cgcaactata ccaaattttt caaatacaat gtccttcctg
     9841 ctacttttgc ccatattttt tctcctttat gcttattgta atcgattact ttttccaccc
     9901 aaataataca tcacttatca a
//
LOCUS       DACCXO010000098         9832 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.100, whole genome shotgun sequence.
ACCESSION   DACCXO010000098 DACCXO010000000
VERSION     DACCXO010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.100"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            89..826
                     /locus_tag="IVY70_002220"
     CDS             89..826
                     /locus_tag="IVY70_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7959335.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            840..1655
                     /locus_tag="IVY70_002221"
     CDS             840..1655
                     /locus_tag="IVY70_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP7959336.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1668..2315
                     /locus_tag="IVY70_002222"
     CDS             1668..2315
                     /locus_tag="IVY70_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7959337.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2331..2987
                     /locus_tag="IVY70_002223"
     CDS             2331..2987
                     /locus_tag="IVY70_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7959338.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3149..3970
                     /gene="racE"
                     /locus_tag="IVY70_002224"
     CDS             3149..3970
                     /gene="racE"
                     /locus_tag="IVY70_002224"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP7959339.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3973..5328
                     /gene="rph"
                     /locus_tag="IVY70_002225"
     CDS             3973..5328
                     /gene="rph"
                     /locus_tag="IVY70_002225"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP7959340.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5329..5847
                     /locus_tag="IVY70_002226"
     CDS             5329..5847
                     /locus_tag="IVY70_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP7959341.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5954..6454
                     /locus_tag="IVY70_002227"
     CDS             5954..6454
                     /locus_tag="IVY70_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7959342.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(6734..7654)
                     /locus_tag="IVY70_002228"
     CDS             complement(6734..7654)
                     /locus_tag="IVY70_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP7959343.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            7819..8832
                     /locus_tag="IVY70_002229"
     CDS             7819..8832
                     /locus_tag="IVY70_002229"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP7959344.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(8942..9697)
                     /gene="fabI"
                     /locus_tag="IVY70_002230"
     CDS             complement(8942..9697)
                     /gene="fabI"
                     /locus_tag="IVY70_002230"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP7959345.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT         3195 a         1715 c         2093 g         2829 t
ORIGIN      
        1 ttacaaatta ctaaactatc agatataatg tattttatta tacatcttta aaagaaatct
       61 tactaaataa taatgggagt gtaattctat ggcaatgatt aaatttgatc acgtggataa
      121 atattatggc aagttccacg cattaaaaaa tatcaatctc gaattcgaga aaggagaagt
      181 tgtcgttgtc attggtcctt ctggttcagg aaagagcacg atgcttcgct gtatcaatgg
      241 attagagacg atctcttcag gtaagctgct gattaatgat actgacttgc atgataaaaa
      301 gacaaaactg acagaagtcc gaaaaaacat cggcatggtt ttccaacatt tcaatttgta
      361 cccaaataaa actgttctgg aaaacatcaa attagcacca atcaaagtgt tgaagcaaga
      421 ccaggcaact gcagtcaaaa acgcggagaa atttttgaaa actgtcaata tgttagataa
      481 gaaagattct tatccttcca tgttatccgg gggacaacag caacgggtgg caatcgccag
      541 aggtttagcg atgaatcctg agatgctgct gtttgatgaa cctacttctg ctttggatcc
      601 ggaaatgatt ggtgatgtat tagacgttat gaaaaaattg gctcatgacg gtatgtcaat
      661 gattgtcgtg acacatgaaa tgggctttgc caaagaagtg gcggatcgtg ttatcttcat
      721 ggctgatgga gaagtattgg aagacagcag aaatgtgaga gactttttcg aaaatccaaa
      781 tgaagtccga gcacagcaat ttatcagtaa agttatcaat cattgatagg aggaaaaaga
      841 tgcgcaggtt aaaaaaaata cgcctgattt tccttttagg cattgctttt cttgcactag
      901 ccggatgtaa aggaaatggg ttggcaaatg aagatatttt agagcgaagc aaatctacca
      961 atgaaatcat ctggggtgtc aagtatgata ctcgattatt tggaatgatg gatattgaga
     1021 gcagaactgt acaaggattt gatgtagata ttgcaaaagc aatcactaaa aagatccttg
     1081 gtgacaatgg aaaaacagaa tttgttgaag tcacttctaa aactcggatt ccattattga
     1141 aaaacggaaa tatcgatgca attattgcaa cgatgacgat cacagatgaa cgaaaaaaac
     1201 aggttgactt ttcagatgtc tatttcgatg ctggccaagc tttgttggta aagaaaggaa
     1261 gccaaatcaa aagtgtagat gatttgaatg cttctacaac ggttctagca gtcaaaggtt
     1321 ctacatcggc tgcgaatatc cgacagcatg caccagatgc aaaaatttta gaattagaaa
     1381 actatgcaga agcatttact gctttacaat ctggtcaggg agacgcgatg actacggata
     1441 atgcgatttt gttagggatt gcagatgaaa atccagagta tgagttagtt ggtggaacgt
     1501 ttactaatga gccttacggg attgcgatca acaaagggca agaaaacttc ttgaaagctg
     1561 ttaaccaagc gctagaggaa atgcatgcag atggcacata cgataaaatc tatcaaaaat
     1621 ggtttccaaa tgaaactgaa ggaaaagtag agtaaggggg agaggcaatg agcgaattga
     1681 tacaaactta tggaccaatc tttgtcgacg ggttcaagat cacattgttt tcgagtgtct
     1741 tagcacttgt gtttagtttg attatcggaa cgttaatggc aatcttccaa ttatcaaaga
     1801 ataaagtagt gagcgcctta gcaaaggcat atgtagaatt ttttagaaac attccattat
     1861 taatcatcgt gatgtttttt tatgttgtcg taccgattta ttggtttaat ctagatggat
     1921 tccaagcagg tacgattgga ttaacgatct atacatctgc ttttattgct gaaacagtac
     1981 gttcagggat tcaaaccgta ccaaaaggtc aaacagaagc aggactatct tctggtttta
     2041 cctattcaga aatcatgcgt tatattgttt taccgcaagc attcaaaatc gttatcccgc
     2101 cattaggcaa ccaatttatc aacctcgtaa aaaactcatc tattttagca atcgtagctg
     2161 gtttagattt gatgtatcaa ggagacttga tcgctagtga aacattcaat accttcgata
     2221 cgtatatcat cgttgggtta ttttacttag ttatcacgct accgttatct tacttaatgg
     2281 tttatcttga gaaaaaatgg gcggttcgtt catagaagga ggggaaatac atggatttta
     2341 gcggcgcttt ttcatggatg aatatccgtt ttttattgga aggattaaag gttactgttg
     2401 aagtttcatt gttttcgatc atttttagtt tcttgatcgg tggtctgaca ggtgtgattc
     2461 gttttgcgaa tatccctttc ttttccaaaa tccttggtgt agtgattgat attatacgga
     2521 atcttccatt attattgatc attttcttca cttactttgc cttacctcaa atcggcattc
     2581 agatgaacat cttctggtcc gctgtcgcag cattaacgat ttttgagtca gctatgttgt
     2641 cggaaatttt ccgagctgga ctcaatgctg ttccaaaagg acagatggaa gcaggattat
     2701 caacgggact gacttatgta gaatcgatgc gaacgatcgt gttaccgcaa gcatttaaat
     2761 caatgatccc ggcaattgtg agccaattga tttcactgat caaagatact tcattagcag
     2821 taatcatttc tttacctgaa ctttctcatc aggcacgtat tatttatgga cagaacacca
     2881 attatgtgct tccgatgttc atcatcatga cacttatgta cttcgtggta tgttacgcgc
     2941 tttctctgct ttccggttac ttggaaaaga gacggtacag ttattaatag atgattcttt
     3001 tagcggttgt ggcaaactaa agtcacaacc gcttttcctc tttccattcg aaaaaaagag
     3061 atttcgattg ttttaagcaa gtaagagcac ttgaaaattc acgctaaaaa gtacacaata
     3121 gggaggatag aatttattga aaaaaaggat gatacgattg acagataatc gccctatcgg
     3181 atttattgat tcaggtgtcg gcggcttgac tgtagtaaaa gaagccctga aacaattacc
     3241 gaatgaaaat attttatttg taggagacac agcacgctgc ccatatggcc ctagacccgc
     3301 ggaacaggta atccagtata cttgggaaat gacggattat ctggtggagc aaggaatcaa
     3361 gatgctggtg atcgcctgca ataccgcaac tgcggtggct ttagaagaaa tcaaagctgc
     3421 tctttctatt ccagtcatcg gtgtgatcct tcccggtact agagcggcag taaaaaaaac
     3481 acaaaataaa caagttggca ttatcggtac gattggtacg gtaaaaagtc aagcttatga
     3541 aaaagcactg aaagagaaag taccagaatt gactgtgaca agtcttgctt gtcccaaatt
     3601 tgtttcagtt gtcgaaagta atgaatacca ttcatcggtg gcgaaaaaaa ttgtggcaga
     3661 aacattagct cctttaacca ctaaaaaaat cgatacattg attttgggat gcacccatta
     3721 tccattatta cgccccatca ttcaaaatgt aatgggagaa aatgttcaac tgatcgattc
     3781 tggagcagaa acagtaggtg aagtatctat gctgttagat tatttcaatt tgagcaattc
     3841 accgcaaaat ggtcggacat tatgccagtt ttatacaact ggctctgcca aacttttcga
     3901 ggaaatagct gaagactggc ttggaatcgg acacttaaat gtagaacata tcgaattggg
     3961 aggaaaataa ggatgcgtca tgacgggaga aaagcccaag agcttcgcaa actggagctt
     4021 caaacaaatg ttttcaaata tccagaagga tcggtagtga tccgttttgg tgatacaaca
     4081 gttatttgtt ctgcaacaat agaaaattct gttccccctt ttttgagaga aacaggaaca
     4141 ggatgggtga cagcagaata tagtatgctg cctcgagcaa cgagtacgag aaatcgtaga
     4201 gaaagcagca aagggaaact ttctggtaga acaatggaaa tccagcgtct tattggtcgt
     4261 tccttgcgtg cagtagtaga cttggaaaaa ttaggagaac gtagtatcat cgtagactgc
     4321 gatgtgatcc aagcggatgg cggtactaga acagcaagta tcactggggc atttgtggca
     4381 ttgaaattag ccattgaaaa acttttaaga gaaaaagaat tgtcagaaga tccaataaaa
     4441 gaacatcttg ctgcagttag cgtaggaatc ttaccagatg ggacttgcgt cacagatttg
     4501 gattaccaag aagattccgc agcacttgta gacatgaatc ttgtaatgac agaatctgga
     4561 aaattcgtgg agattcaagg aactggagaa gaagcaacgt ttgatggtga acagttgaat
     4621 gaaatgctct tttttggaaa aaacgcgatt gaagatttga tcaaagagca aaaacatgct
     4681 ttgctaacag agtttgctca aaatgatgag cggatagaag aaacaaaaac aatcatcatt
     4741 gcgacaagaa atccaggaaa agcagaagag tttcgaaata tgttcaaaga agcgggatat
     4801 catgtaaaaa cattacttga ctatcctgaa ttgccagatg tggaagaaac tggcagcaca
     4861 tttgaagaaa atgcccgatt aaaagcagaa acgattgcac aattgcttga tcagccagtc
     4921 ttagcagatg attcaggttt gaaagtggat gctttaggcg gtatgccagg aatctattct
     4981 gcacgttttg caggggaaca aaaaagtgat gcggggaata acgccaaatt attatatgaa
     5041 ttgacagatg tgccggatga aaaaagaaca gcacaattcc attgtacgtt agtattcgcg
     5101 gcacccaaaa aagacagcct tgtagtagaa gcggaatggc ccggtcgagt agcccgtata
     5161 ccatctggcg aaaatggctt tgggtatgat cctttattta tcccagaagg taagaaacaa
     5221 acagcagcag aattatcgag cgaagaaaaa aacaagatca gtcatcgggc tcaagcaatg
     5281 aaaaaattga gtgccgagtg gaaacaatgg ctggaaggag agagatagat gcggtattta
     5341 gtagtaagtg ataatcatgg agacagagat atcctcgttg acttagtaga tcgttatcgt
     5401 tctgaagtgg atatcttttt ccattgtggt gattcggaat tagaagcttc tgacccatta
     5461 tgggatacat tccaagtggt tcaaggtaat tgtgattatg gaccaggatt cgaacagaaa
     5521 aaagtgatcc aaactggtca ggatacagta ttcatgactc acggtcattt atcgaatgtc
     5581 cgatttggtt tgacacagtt agcaatcgaa gctcgagctg caactgccga tatggtgttt
     5641 tttggtcata cccatcaaat aggctgtgag atggatcaga aaatcctgtt tttgaatcct
     5701 ggtagcattt ctcagcctag aggcccgatc caaatcccat catatgctct tatcgaaagt
     5761 ttagatgacc aaatttctgt tcaatactat aaccgtgccc atcaggcagt tgaagagttg
     5821 gccttttctt tttcgagaga gcgctgaata aaaaaactct aatttttaat aaaaataagc
     5881 tttattttat gaattttgca tccggtttcg gtaaaatagt agtgttgaat gaaatagaaa
     5941 tggaggccat cagatgattg gacctatagt aagagaaatg ctgctggaaa atcaggaaac
     6001 gtttttgatt cctgcagaaa atgtggcaaa tgttatgtat catcatccat tgtcccatgg
     6061 tttacttgtg ttatcgaagg tcggttattc caaaattcct gtattaggaa aagatgatca
     6121 ctttgtagga ctagtaagtt tatcagatgt ggtcaataaa atgattgact tgcaaacaat
     6181 cagcatggac ccgttagaag gactaacggt tgctgatgtg acagaaacag atgttccgac
     6241 aattgatgaa aattgggaac tagaagaagt actccatttg ttagtcgatt cgcctttttt
     6301 accggttgtc acagaagatc ggattttcaa aggaatcatt acaagaaaag agctgcttaa
     6361 agccgtaaac ttcatggtgc atgaattaga acgtcgtaat atattgtctc cgaaaatcga
     6421 agaattaaaa gaacaaatcg atatcgtgag ctaaagaacg agaaactaaa agataatcaa
     6481 aaaataagta gaaaaaacag cgtaccagtt ttgactttat cttgaaaaag aggctgggac
     6541 aaaagcgttt aactccaaga aataagaagg aattcacgaa aattgctttt caaatttttg
     6601 tgaatttcag cttatttccg aaggagttgc ttctgcttcc gccgtttata cgtgtttaga
     6661 gcgtgggaca aaagtgttct ttacttttgt cccacgctct ttttagtgag attcgttgca
     6721 tcgtttgtcc aaattatttt ccgacagcaa ttggactgtt cggaatcgtg aaaccagaag
     6781 ctgtcaattc tttgacatag gagctcaaga actcttcttt taaggcatat tgttttccgt
     6841 tcaatgcgta gcagaccgta cgaatcgcaa aattgctgtt tccaagatcc actaatccaa
     6901 agatagaagg ttcagtttgg agctcttctt ggtatttttc agctaatgta cgatttactc
     6961 gatcaatgat ctcatagatt ttgtcatatc cttcttcagg aacaatccga atatctaaaa
     7021 gtacttgcat atttgcacga gagagattgc tgatcgtggt gatgtttcga ttcgggatga
     7081 aatggacagt accatccgtt gctttcagct gaagggtacg gataccaaca gatacgacgg
     7141 ttccttcaat cgttaagttt gctagcttga tgtagtcccc aacatccatc tgttgttcca
     7201 tgataataaa aaatccggta ataacatcac tcatgaaccc ttgggcacca agaccgattg
     7261 ccacacctgc aattccggca ccagccaaaa gtgatccgat cggcacaccg atagcagata
     7321 aaattgcata gataaaaaag aaaccaaggg tataatgaaa aatagtattt aaaagactat
     7381 gtaaagtctt taagcggctc tcgtttagaa tggctttttt agaatactga ttaaaagact
     7441 tatcaatcag atattttccc acacggtgaa gaatccaaaa aaggacgatc aaaaaaatca
     7501 gatataaact tttttggata aaggcagcca aaatggcgtc ccaatcaatc ccgtcccaaa
     7561 accgctgaaa cgcatttact ttgctgacag cttgatctgt caatgtctcg ccagaagagt
     7621 ctgtcgtacc ggcagttgct aataatgaaa acataaaatc ctcttttcta aaatttgatt
     7681 ctttctgtat tgtaccaaga agttttcaaa aaaggaaata gcaaaaataa ctataatgaa
     7741 ggaaaaaagc tgaaaataaa atgacaaata atatggataa tgataaaatg tggagaaaat
     7801 aacgaaggtg gtaagtatat gcaaatcgat tggaacaatc taggtttttc ttatatcaaa
     7861 actccttggc gttttattgc caaatggaaa gacggacaat gggaagaagg agaattgaca
     7921 gaagataatt acctgacgat tcatgaaggc tctccagcgc ttcattatgg tcagcaatgt
     7981 tttgaaggtc taaaagctta tcgtcgaaaa gacggcaagg tcaatctctt tcgacctgaa
     8041 caaaatagtc gacggatgaa tcaaagtgct agacgattgc tgatgccaat ggtccctgaa
     8101 aagatgttcg ttgatgctgt caagcaagtg gtaaaagcaa atgaagattt tgtgccgccc
     8161 tatggaacag gcgctacatt atacttgcgc cctttactga ttggggtcgg tgaaaacatt
     8221 ggtgtccatc cagctccaga atatttattt gtcgtcttct gtacgccagt aggagcgtat
     8281 ttcaaaggtg gactaaaacc aacgaatttt attgtatctg attacgatcg tgctgctcct
     8341 aaggggactg gtgcagcaaa agtcggcgga aattacgcag cgagtttact tccaggaaca
     8401 gaagcaaaag aactggatta ctcagattgt gtttatcttg atccagccac acatacgaaa
     8461 atcgaagaag taggtgcagc aaatttcttt ggaattacaa aagacggaaa attcatcact
     8521 ccgcaatcgc cctctatctt accaagtatc acgaaatatt ccttgttgca gttagctgaa
     8581 gaacgactag gactgcaagc aattgaagga gatattttca ttgatgatct agatcaattt
     8641 gcagaagctg gtgcatgtgg tacagcggct gttatttctc caatcggtgg gatttatcac
     8701 aataacaatc ttcatgtatt ttatagtgaa acggaagtag gaccagtaac aaaacggttg
     8761 tatgatgaat tgacaggtat tcaatttgga gacatagaag caccagaagg ctggattgaa
     8821 gtcgtggaat aatttcaaag taatgagttg aaaaaaacgt aaaaagaaaa aatcaggaag
     8881 agactttaaa aagttttttc ctgattttta tatgttttac tttaaagaat gcaacaaaag
     8941 tttatgaaag atggacacct ttgtcaacgt agataatatc accgatgatt ccgctggaaa
     9001 gtgggctgat taagaaagca caagcattac cgacttcttc gatcgtcaca ccttgctgat
     9061 caggcgtacg agattcagat aactggatca gtttttgata gtctttaaca cctgtgacag
     9121 ctaatgtttt gattgcccca gctgagattc cattgacccg aattccttta ggcgagaact
     9181 cagctgccaa ataacggata gcagcttcaa gagaagcttt cgcaataccc atcatgttat
     9241 aattcgggat tgcgcgttct gaacctagat aagtcatgct aacgatcccg ctgtttggtg
     9301 caagaaacgg ttgtgcgtat tttgatacag caattaaaga gtagctgctg atatcttgag
     9361 ctaattgata gccgtctctg gaaatatcag acacatttcc ggacaattct tctttgttgg
     9421 cgtaagcaac tgcatggaca aggccgtgga tctccccagc atattcaccg atggcattca
     9481 ttgctttttc gatgctttcg tcgctagaaa catcacattc tacagtgaat gcttcttcgc
     9541 cgaccaattt gactaatgat tttttcattc gttcattttg gtaagtatag ataatttctg
     9601 ccccttgttt caacattgct tcagcacaac cccaagcaat gctttttttg ttagcgacgc
     9661 ccataatgac gatttttttt ccttctagaa atgacataat gactctcctt tgtttctgat
     9721 aaaaatttga tagtcgtaaa aaaatagaga ataatagcta aaataacttc ttgatttctt
     9781 aattattagt caaaatgttt tgattgtcaa actatttttt cttaaattaa aa
//
LOCUS       DACCXO010000099         9821 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.101, whole genome shotgun sequence.
ACCESSION   DACCXO010000099 DACCXO010000000
VERSION     DACCXO010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.101"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(80..2200)
                     /locus_tag="IVY70_002231"
     CDS             complement(80..2200)
                     /locus_tag="IVY70_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP7959346.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2430..3608
                     /locus_tag="IVY70_002232"
     CDS             2430..3608
                     /locus_tag="IVY70_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP7959347.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3624..4382
                     /gene="aroD"
                     /locus_tag="IVY70_002233"
     CDS             3624..4382
                     /gene="aroD"
                     /locus_tag="IVY70_002233"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP7959348.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4405..4890
                     /gene="ybaK"
                     /locus_tag="IVY70_002234"
     CDS             4405..4890
                     /gene="ybaK"
                     /locus_tag="IVY70_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP7959349.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4961..6214)
                     /locus_tag="IVY70_002235"
     CDS             complement(4961..6214)
                     /locus_tag="IVY70_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7959350.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6330..7679
                     /gene="gdhA"
                     /locus_tag="IVY70_002236"
     CDS             6330..7679
                     /gene="gdhA"
                     /locus_tag="IVY70_002236"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP7959351.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7791..9137
                     /locus_tag="IVY70_002237"
     CDS             7791..9137
                     /locus_tag="IVY70_002237"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP7959352.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9445..>9821)
                     /locus_tag="IVY70_002238"
     CDS             complement(9445..>9821)
                     /locus_tag="IVY70_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7959353.1"
                     /translation="LGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAETDIK
                     ADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKASEKRI
                     IEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3137 a         1788 c         2010 g         2886 t
ORIGIN      
        1 attattctaa gatttctctt taccaacatc atttgacaaa gagcctaatt ttgtcgctgc
       61 tccttttaaa tttattttat cattcggaag aacttgtggt cgttccgtga agttcttggt
      121 acgtttgcgt tttataaaga tcaacagttg attgtccctt gtgttcacta aagacactag
      181 ttgacttgtc accaagagat ttttcagctt cttttaattt ctctaagcca ttcttgtagt
      241 cataatcact ggtatcaatt gggtctaatc cactattcgt atagaaccga agaagatcac
      301 cattattgat ttggtcagaa acagctaact gtttatcaac tttttctttc agtgaatcaa
      361 tttcattctg tacttcctca gtaggttcag taatagcttc accagtttcg ttactataga
      421 tcgtaccacc gtagtaagta tattttggtg taacaaaatc accatcacgg aaagaaacaa
      481 gctgttcatg atctttcgag aatagatctt gtcctaactg aatataattt tttgtatcta
      541 cacctagtaa gtgaagtaat gtaggcaagg catcaacttg tccgccgtaa gtgtgattga
      601 ttccgccctg tgtttgtcca gggatatgga tcatataagg gacacgctgc atttgtgcat
      661 tgtcgtagtt tgtccaagta gaacttgttt ttccaactaa ttcagctaga tttttatttc
      721 ttgaattcga gacaccgtaa tggtcaccgt aaaggacgat gactgaatta tcatacaagc
      781 cgctttcttt caaataattg aagaattctt gtacggcggt atctaaataa ttggctgtcg
      841 caaagtaacc gttgatcgtt tcatcatttg tattggctaa cgggaatcct gcttcgtcat
      901 tggtgaattt tgaataagga taatggttag atacagcaat gaattttgaa taaaatggtt
      961 gttgcagacg ttccaagtat tgtacggatt gttggaaaaa tggtttatca tgcaacccgt
     1021 attggaacga attgtcatca ttaacatcat aataagaaga atcaaagaaa taatcatatc
     1081 ctaaattctt gtatgtctcg tttcgattcc agaatgtacc agcgttccca tggaataccg
     1141 cactagtgta accttgtgtt tgacttaaaa tatcaggtgc agcttggaaa gtattttttc
     1201 cgcctaattg tgtgaacaag gagccttgat tcaagccaaa caatgaattt tccatcagcg
     1261 tttccgcatc gcttgttttt cctgctttta cttgatggaa aaagttgtca aaactgaacg
     1321 tgctgttgct gtggtaaaga ctattgatga aaggtgtaac ttcaagctgg agaccattct
     1381 catctgtcaa tttataatcg atcaggaatt gctggaagct ttctaaatgg atataaatca
     1441 cattttttcc tttagcgagc ccgaacaaat catcatttgg tgctgcataa tgttctttta
     1501 cataattttc tacttctttc atatcattgg gacttgcttc tgcccgcact tggtttgtct
     1561 ggtaagtttg aacaccgtca taaaccatga aagcattcaa gccaagatat ttgaccaaat
     1621 aatcacgaga gaatgttcga gtcagcaatt ctggacgatc agcttccgcc ataaaaagat
     1681 ttcctgagaa aatcataact gctaatgtag agatcgcaaa agccattctt gctcggatcg
     1741 ggcgagggtc caacttgatt ttttttgcaa tcagcagaac agtcaagata ataaaatcga
     1801 tccaatagat cacatcatat gggcgaaaca gtcgaatggc actttctccc agaccactcg
     1861 ctactttccc agcacctaaa atcgtgttga tcgtaatgaa gtccgtgaat tctcgataat
     1921 aagtgacatt cgaaaaaaca agcaatgtca aaagaaaata aatgaccatc atcgtgatat
     1981 aagcaactct agggcgtctg atataaagag caattccaag taataacaat gtagttgcaa
     2041 tagggttgat aaacaagata aagtattgaa tgccactttc gattccgaga tggaaatcaa
     2101 ctaaataggc aaaaatcgtt ttcaaccaaa gtaaaacaac tagtaaagaa aaaaatccga
     2161 gcctcttatt caaaaaggca ggtgttttta tacatttcaa gataatcttc ctttctgcac
     2221 ttcttaatca cagaagtgtc atgaaaccat gttacacagc tcacttattc tcatttattt
     2281 tactagtatg acaatatagt gtcaattgaa accagaagtt tatgctgttt cattaaggtt
     2341 ttctttaagc ttcatgaaaa aatgagcagt aagacaagtc ctatgttttt tgttatactt
     2401 gggaagagaa agctaaagca ggtgaaggaa tgaaagtaaa agtaaaaaaa agagcaagca
     2461 atcgattgaa aaaaggatat ccactgatcc aaaaagaaga ccttgctcat ccccaaaaag
     2521 aaaccacgca atgggtggaa ttagttgatt cgcaaaacca atttatcgca aaaggatatc
     2581 taggaaagca gaataaggga atcggttggg tattgagcca gcagaacatc ccatttgatc
     2641 aatctttttt tgaagaattt tttgtgaagt caaaagaaaa aagaactttc tttttttcag
     2701 atgattctac taccgctttt cgtatattca atggagaagg agacgggatc ggcggtctga
     2761 ttgttgatta ttatgcaggt tatgctgttt tttcttggta taacgaaacg ttatatgcac
     2821 atcgtgaatt attgattcaa gcttttcgtg aaatctaccc ggaaatcgta ggaggctatg
     2881 agaaaattcg atttgaatca gtaaatctgc ctgagagcca gttcatttac ggaaaagagg
     2941 caccggaacc cttgatcgta aaagaaaacg gagtaaaatt tgccgtttat ttaaatgaag
     3001 gattaatgac tgggatcttt ttagaccaaa aagaagtacg gggatcatta gcagaaggtt
     3061 tcgcttctgg gaaaacagtt ttgaacatgt ttagttatac cggcgccttt tctgttgctg
     3121 ccgcaatggg aggggcaatc gaaacaacaa gtgtcgatct agcaaaacgc agtttaaaaa
     3181 aaacacagga acagtttgaa gtgaatggtt tggatgtcga tcagcaaaaa atcattgtta
     3241 tggacgtatt cgagtatttc aaatacgcca aaagaaaaca atttttatat gacatgattg
     3301 tattggatcc accaagtttt gccagaaata agaaaaaagt ctttcgcgta gcaaaagatt
     3361 atggggaact ggtcaaggat agtctggata ttttagcaga agacgggata cttattgcgt
     3421 ctacaaatgc tgcgaatgtc tcgatagaac aattccaaac aatgattgaa gaggaattcc
     3481 atgccaaaaa tgtttctttt gaacaacttg ctttatatcg tctgccaaaa gatttccaga
     3541 cgacggatac ctttttagaa ggcaattatc tgaaagtgtt tgtttatcaa attaaaaata
     3601 accattagaa ggagcgatgc agaatggttt gggtaaaggg attgaatata ggaagcggta
     3661 gaccaaagat ttgtgcgcct cttatcgggg ccgtccgaga ggaagtatta cgtgaagcag
     3721 ttcttgcacg tgaagcaggg gccgatttag tcgaatggcg agtcgatcat tatcgtgaag
     3781 ttttttcttt tgatgaagta gcagaaacat tggttcttat ccatgagatg cttgatggac
     3841 tgccgttatt atttacgttt cgtaccttag ctgaaggcgg agaacaagac atatcgatcc
     3901 gtcagtatcg cgacctttat gaacatgtga tacacacagg attagtagat atggtcgata
     3961 tcgagttgtt caaagtagaa agtctaggaa aaagactgat agaggaaatc aagtctttaa
     4021 acattccttt aattatcagt agtcatgatt ttaaagaaac accagcagac cctgtcttgc
     4081 tttaccgatt gaatgttatg gaacattttg gagccgatat cggaaaactg gcagtaactc
     4141 ccaataatga acgagatgtt ttgcggttga tggagttgac tagacgggca aatgcttttg
     4201 tctccatgcc tattattacg atgtccatgg gaaatcttgg taaaatttct cgtctggctg
     4261 gcggaacgac gggttccgtt atgacatttg gttccttaag cagcgattct acttctgctc
     4321 caggacaatt accggtcaaa gcattgaagc aaattattca attattaaat gaaccgttgt
     4381 agaacaagtt tgaggtgata ttttttggca aagaaaaaaa tagtaaaaac gaatgcaatc
     4441 cgtatggtcg aacaaaaaaa gattccctat acagagcacg aatatgagtg ggacgaaagc
     4501 cacctaagtg cgtcatcggt cgctgaacag cttccagaaa gccaatctca tatatttaaa
     4561 acactggttg ctgtgggcaa tgtgacgggg cctcttgttg ctgtgatacc gggagaagcc
     4621 gaattgaatc taaaaaaatt agcaaaagtt tcagggaata aaaaagtaga aatgctccat
     4681 ttaaaagatt tggaagcaac gaccggatac atacgtggtg gctgctcgcc tataggtatg
     4741 aaaaaactat ttcctactta tttagatcaa attgcagaaa gctatgaaca aatcattgtc
     4801 tcggctggca gaagagggct acagatggag ttagcacctc aagatatctg tgcattaacg
     4861 agcggacaat ttgcagatat aaaacaataa atcatacgta taaaagaaag aaggaggccg
     4921 ggatgttgtt tgtccagtct ccttctttct tttttataca ttatgctctt tgcccggcat
     4981 tcattcctgc tacatgccct gtagcgaaag ccgctgtgat attaaagccg ccagtataac
     5041 cattgacatc cagtaattct cccgcaaaat acagaccatc aatgatttta ctctccaatg
     5101 ttttaggata aatttccttt agagaaaccc ctccgccagt tacaaatgat ttctcaatag
     5161 gaaaagtttt gttgattgat agcttaaact ctttgcatag ttgaacaaaa tcttgaattt
     5221 gtttttgaga ggcttgttgt cctgtcagat gatccatttc aagccgctca agaaagaaaa
     5281 ctaacaatcg ctcaggcaat ataccatgcc aagcattgac caagttcttt ttggttgctt
     5341 tacttttttc tgtcaatata tgaataagct cttgtttggt ttgtgtaggg aaacaatcca
     5401 gagaaagtgt cactggttct cccgttttct ctaattcttt attgacaaaa cttgaacaac
     5461 gcagtgcagc cggaccagaa ataccgaaat gggtgaaaag taaatccatc gtatgttcag
     5521 tcaatacacg acctttttta tttaaaacgc gtaaagtgat gtcttgtaaa gaaagccctt
     5581 gaagtgtttt ctcctgaata tacggttcat ccgatatcaa gggggactct gtcgcataaa
     5641 gaggcgtcac cgtgtgaccc actcgcttca cgatcttgta cccatctcca gtagaaccag
     5701 tagatggata agtccgtcca ccagttgtta agataatggc aggagcatgg aactcttcga
     5761 attccgtgcg tataccgtaa attttatgtt ctttgtggat caatttctct actcgagtag
     5821 agaatagaag cgtcacatct agttctttca gacgattgag caatgcttcg ataatcgttt
     5881 ttgatttatt tgttacagga aacatccgtc catgatcctc ttcttttaaa tgtacgcctt
     5941 gtgattcaaa gaagttcatg atatcgaaat tattccattg cgcaaacgta ctatataaaa
     6001 actttccatt tccaggaata tgtgcaatca aatcatcaac aggacgattg ttcgtaacat
     6061 tgcaccgacc accacctgtc atcagtagtt tttttcctgc ttttttgttt ttttcgatca
     6121 agagtacgcg tgcgccttgt tcagctgcac tgatcgctgc catcatcccg cttgttccag
     6181 cgccgactac gatcacatca tacagttttt ccattccctc acctcaagga aagtgtagca
     6241 tatctgaaga aagaaaacag aaaaaatgtt ttgagccaaa agagaaaatg agctaaaatg
     6301 agggaagata agcaaaggag cgtggaacga tgacaaacgc aacagaatat gttcaagcaa
     6361 ttcaagaaaa gctacatcaa aaggatcaag ggcaagaaga atttttacaa gcaatcgatg
     6421 aattcatgcc cacggttatg tcttttttgg acactcatcc cgaatatatc gaaaagaata
     6481 tcttagaact tttaacggaa ccagaaagag ttattcaatt tcgagtgcct tggcaagatg
     6541 atgaaggaaa ctggcgagta aatagagggt accgtatcca atacaactca gctatcggcc
     6601 cttataaagg tggattgcgc ttccatccta gtgtcaatct aagtatcttg aaatttttgg
     6661 cttttgaaca gattttcaag aatagtttaa ccggtctgcc aatcggtgga ggaaaaggcg
     6721 gaagtgactt cgatcctaaa ggaaaatctg ataatgagat catgcgtttt tgtcaaagtt
     6781 tcatgctgga attgtcaaaa catatcggcc catcgattga tgttcctgcg ggagatatcg
     6841 gtgtaggagc tagagaaatc ggctatttgt tcggcgaata taaacgattg aaaaaatatg
     6901 atactggtgt gttgacaggt aaacctttgg atttttgggg aagtaagata cgtacggaag
     6961 ctactggtta tggattagtg tattatgtca aacatctatt gaacgaagaa aaagattcat
     7021 ttaatcaaaa aacagttttt gtttccggaa gcgggaatgt agccatttat gcaatcgaaa
     7081 aagtgcaaga gctaggcggg aaagttgtta catgttcaga ttctagcggg tacatctatg
     7141 atccagaagg aatagatgtg aaactattga aagaagtcaa agaaatcaat agacaacgtt
     7201 tgacggaata cgcaaaccaa cgtccttctg ctatttatca tgctggagaa tctgtttgga
     7261 cattgaaaga aaaagcggat atcgctttgc catgtgctac tcaaaatgaa atagacgaaa
     7321 gcttggccga aatcttagta gaaaatggga tcaaaatcgt agcagaaggc gctaatatgc
     7381 catgtacagt aaaagctgtt tctgttcttc atgaagcagg cgtgtggtat tgtccaggaa
     7441 aagctgctaa tgctggagga gtagcagtat ctgctttaga aatgagtcag aacgcccagc
     7501 gattgacttg ggaaagagag caagtggatg agcagctgga tcaaatcatg gaaacgattt
     7561 accatacgtg tcgccaaaca gcagccgagt acggcgacga aaaaaatctg cttcttggag
     7621 caaatgttgc tggatttgaa aaagttgcca aagcaatggc agcagaaggt ttagtttgaa
     7681 aaatgaaact ttcttggaaa tattgtcagc attttgttta tttgcttgaa aaataataaa
     7741 aaaaaacgta aagtattacc tgtagagtaa acctataagg aggagaacaa atggcacaca
     7801 ttcattttga ctattcaaaa gtagcaccgt ttgtcaacga acaagagtta gggtacatgc
     7861 aaagccaagt aacagcagcc cacaacgaat tacgggaagg tactggcgca ggaagcgact
     7921 tccgcggctg gatcgattta ccaacgaact atgacaaaga agaatttgca cgtatcaaag
     7981 aagcagctaa aaaaatccaa tctgattctg aagtattagt tgttatcggt atcggtggtt
     8041 cttatttagg cgctcgtgca gcgatcgact tcttgaatca cacattcttc aatttattag
     8101 atagcaacac aagaaaagca ccacaaatct tctttgccgg aaattcaatc agctcaacat
     8161 atattgctga cttgatcgaa gtaatcggtg atcgcgactt ttcagtaaac gttatttcaa
     8221 aatctggaac aactactgaa ccagcgattg cgttccgtgt attcaaagag cttcttgtta
     8281 agaaatatgg acaagaagaa gcaaacaaac gtatctacgc tacaacagat aaagcaaaag
     8341 gtgcagttaa agtagaagca gatgcagaag gttgggaaac tttcgtgatt cctgatgaca
     8401 tcggcggacg tttctctgta ttaactgctg ttggtttgtt accaatcgct gttagtggtg
     8461 cagatatcga tgcattgatg caaggtgccg ctgatgcaag caaagcatac tcaagcgaca
     8521 aactagaaga aaacgaagct taccaatacg cagcaatgcg caacattctt taccgtaaag
     8581 gcaaagtaac tgagttgttg atcaactatg aaccaggaat gcaatacttc tcagaatggt
     8641 ggaaacaatt gtatggtgaa tctgaaggaa aagaccaaaa aggcatctat ccttcaagcg
     8701 caaacttctc aactgatttg cactctttag gacaattcat ccaagaagga cgtcgcaata
     8761 tttttgaaac agttgtgaag gttgaaaaac ctcgtaaatc aatcacaatt cctgaacaag
     8821 cagaagattt agacggtcta ggctacttgc aaggaaaaga agtggacttc gtaaatacaa
     8881 aagcatttga aggaacattg cttgctcata cagacggtga cgtgcctaac ttgctagtca
     8941 aaattccaga aatggatgct tatacattgg gttatacaat gtacttcttt gaaattgctg
     9001 taggtatttc aggttacttg aatggaatca acccattcga ccaaccaggt gtagaagctt
     9061 acaagaaaaa catgttcgca cttctaggca aaccgggctt tgaagatctt gctaaagaat
     9121 tgaacgaacg cctgtaagat aagggttttt cgctagattt aaataatcag tgatagaaac
     9181 atgttcgctt taaaatgaaa actggtaccc atttaggtac tcaaacatac tgcatcgttt
     9241 attcagaata aggggctgtg acaagacgtt tgtcacagtc ccttgtttgt atagaaatcg
     9301 attgattgcc ttgctcttta gtttgtttta ggactttaag aacagtgagg ctgacttcga
     9361 aggttaattc tgtttttttg ttttctagaa tcagtaagca aaaaagcact cacttcgagt
     9421 gcttttttgc gatatattct tagcttaaga taggcctaat ttttctttta cagatgaggg
     9481 taccttatcg ctggtaatta cttttttctc tatgatccgc ttttcgcttg ctttcaactg
     9541 caagatcgtt ccttcggtgt aagcagaacc attggcagag tcaccagaag taatgacatc
     9601 gaaatctaac tgtctttttt caccgttttc agcataagca tctacgttgt atttaaaacc
     9661 tcttccaatt acttccccat tatctgcttt gatatcagtt tctgcaggca ttggagcttt
     9721 gactaccgca taataatctt tacctacata agtagactga tagtagtccc aacctttcca
     9781 tcctataaaa gcaatcaata tgataacgat caaccctaaa a
//
LOCUS       DACCXO010000100         9746 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.102, whole genome shotgun sequence.
ACCESSION   DACCXO010000100 DACCXO010000000
VERSION     DACCXO010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.102"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(591..989)
                     /locus_tag="IVY70_002239"
     CDS             complement(591..989)
                     /locus_tag="IVY70_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959354.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KIAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1205..2260
                     /locus_tag="IVY70_002240"
     CDS             1205..2260
                     /locus_tag="IVY70_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HAP7959355.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTANLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2280..2633
                     /locus_tag="IVY70_002241"
     CDS             2280..2633
                     /locus_tag="IVY70_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959356.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(2815..3111)
                     /locus_tag="IVY70_002242"
     CDS             complement(2815..3111)
                     /locus_tag="IVY70_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959357.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3115..3933)
                     /locus_tag="IVY70_002243"
     CDS             complement(3115..3933)
                     /locus_tag="IVY70_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7959358.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAVNENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWMVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4159..4380
                     /locus_tag="IVY70_002244"
     CDS             4159..4380
                     /locus_tag="IVY70_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAP7959359.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
     gene            4382..4648
                     /locus_tag="IVY70_002245"
     CDS             4382..4648
                     /locus_tag="IVY70_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7959360.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            4761..5300
                     /locus_tag="IVY70_002246"
     CDS             4761..5300
                     /locus_tag="IVY70_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959361.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGAYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKQQLDRIKEENNKPNLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            5794..6078
                     /locus_tag="IVY70_002247"
     CDS             5794..6078
                     /locus_tag="IVY70_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959362.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGVVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKNSFIGKLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            6352..6606
                     /locus_tag="IVY70_002248"
     CDS             6352..6606
                     /locus_tag="IVY70_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959363.1"
                     /translation="MNYTDEEQITLEYVFTNEKGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            6688..6873
                     /locus_tag="IVY70_002249"
     CDS             6688..6873
                     /locus_tag="IVY70_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAP7959364.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            6940..7335
                     /locus_tag="IVY70_002250"
     CDS             6940..7335
                     /locus_tag="IVY70_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP7959365.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEDLLEK"
     gene            7325..7702
                     /locus_tag="IVY70_002251"
     CDS             7325..7702
                     /locus_tag="IVY70_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959366.1"
                     /translation="MKSKKETEPMKSTHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            7703..8098
                     /locus_tag="IVY70_002252"
     CDS             7703..8098
                     /locus_tag="IVY70_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959367.1"
                     /translation="MKQEKRKMKQTQTEPINDTLADQLAGYYKLFEMSKAQRLIHYLW
                     KYKILFLFWEATLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
     gene            8413..9708
                     /locus_tag="IVY70_002253"
     CDS             8413..9708
                     /locus_tag="IVY70_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7959368.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT         3369 a         1700 c         1594 g         3083 t
ORIGIN      
        1 aaatatttta tttttgttat tttattagca ggaaaattta aatagtttta caagaatccg
       61 tagaaatata ttggttttgc cttcagatta atactggatc atattaatct gaatataata
      121 tatgaactaa taaatagctt aacctcgcta ttttgttttt tctgatcact atctatacag
      181 cattagtaaa tagaatttta aaaataaaaa aattaagaca aaaaagtatc aacataaaca
      241 tatacatata cacgtttatg ttgattcact taatttgtcc tttaaaaagc acttatttta
      301 atataatgtt attttataat cttattacaa gctgattgtt aggcgttgtc ctttcattac
      361 atttattaac ggttatttta ttgtttaatt tattaattat ttttgatcta aaacttagct
      421 taacaaataa ataattaacc aaccgccaga taaaaatgcg atcattgcta atacataagc
      481 aaagataaaa atatgttcga tcagcttaaa ccctgatgcg attaagcaga ccaagtaaaa
      541 tagaaattct cttacagata atggtaattt tctaactagc atactcataa ttaacacccc
      601 aaagtaatat tgtattttga acattttaca tccaatacag catcaatcaa ccattgccaa
      661 tcaaaaatac aaggcttcat tgaagtatgt ttttcagatg aaaagatata atataatttc
      721 ttcggaataa tattttcagt tgttgggatc aagctaaatc taataccgtc taactcatag
      781 ctaccagctt tctctccatt aaaaatttcc ttagcacttt cttctaaaga atcaaaatat
      841 ttttcgacag caatctttga agcttttact attgaagcat ttcccttttc gataaaatct
      901 gcttttgttt taccgacaat aagtaattct tgatcagtaa aatcttctaa aataatggta
      961 gtgtaattct tatttcttct caatcgcatc aggatcactc cttaatataa tttatgtttt
     1021 tataatatca taataagata aatttgcaag catttattta caccttttta tttacacttt
     1081 attataatta attagacttt tatgattgca tttttttctt ataaacagtt gcaactaata
     1141 gtaaataggt gtaaactatt ttcgtaccga aataccataa acacaaacag aaagaggtca
     1201 taccatgaaa gaaccattat tgttttccgt tgacttaggg aataaatcag taaaagaaat
     1261 gagtacacac gtttctccac tctcacttcc ctctcgtatt ttgaacaaaa atatcattaa
     1321 ttcaggacgt ttttctaata taggttcctc attaaaacga tcactaaacg tttctgaata
     1381 tagcctagta aacactactg aaagctatta ctttgggaag gatatacacg agctaggaaa
     1441 agatgactac gtgatagaat cactaggttt tgggatcagc agatacaagc aggaaaccta
     1501 ccaaaatatc ctatctttct ctttagctaa cctagcacgt ttagaagatc aatctgatga
     1561 cgttttaacc gctaatctag ttttaggtct gccttcatcc gactataccg aaacaatcgt
     1621 caaatacgtg atgcaacaag ccacacgaca acattcagtt gaagttgacg gtaaagctta
     1681 taatataaac gtagataccg tttgggtact gccacagccg attggaacac tatataacct
     1741 cctacttgct gatgatggta gtgtacaaga tgaaacattg atcagtcaga agatcggttt
     1801 tgttgatatt ggcggtggca cgaaactttt tgatgttgtc caaaattttg aaattgacga
     1861 aaacaatcgt gaacaaaagg ataccggcat ttttacttta tataatcgta tcgtaaatat
     1921 gcttgacctt ccgatcagtc ctaatgcttt ccaacttgaa aagattatcc gagaaggcaa
     1981 agaaaaaggc gtatatctat ttaagccaag cggaaatgaa agttttgata tttcagaagt
     2041 agttgaaaga gaaataaaaa catacaccaa tatggttgtt gcagaagttt tagctacatt
     2101 caaaaacttc acaaccttag ataaagttgt ctttactggt ggtggagctt ctattatcga
     2161 caaacaactt gtaaaagata aaattccgca taccttgttt gttgaagaca gtgaattttc
     2221 taatgtaaaa ggcttttaca agtttggttt aactcaataa aacagcgaag gagatcaaca
     2281 tgaataaggg aaaatcagag cgttatgtag ttataatcaa tagagaaaag gttagcgagt
     2341 tacctttagc agagtttctt gaatccgaat atgaaacact aggcgttaaa tactctcaga
     2401 cgatcaaaaa agctttgaac ctatatcggc gtgtaaagaa caatgaagtt acaattattg
     2461 aagcaggcaa cgatagaaac gatagcaaag tagcagaagt aaagaataaa ccaactgatc
     2521 ataaccctat tcaacaagag catccttcaa cggttacatc cgatcattca gcaaagaaag
     2581 aaaagccaat gctgaaaggg ttcaatgcac caagtaacaa agacttactt taaaaataaa
     2641 aaggcgaggt tacaatacct agccttttta ttatgtacaa atataaacat aaacatatac
     2701 gtatacatgt atataaacat gtatacgtat atatccataa acacgtatat taaatttagt
     2761 taagctatac ctaaccgctt attccaataa aaaaagtgat cagctttaaa ctgatcactc
     2821 accaaaaact tcatcataca agttatctat catttcacca agtaaagctg aatctgatcg
     2881 aattcctaac ttttcgctga ttttagatag tttctttctt tgcgatggtt gaagcgtgaa
     2941 ttgataattt ttcttctttt cagaactatc actttttagt ttagctacca aagaacttgt
     3001 tgtttcttta ggtgtttgtt taggttcttc aactgcttca accacttcag gaacttcttc
     3061 tacttcagct tcagcgaatt ttttaaatct attttctttc acttcaatca ctccttattc
     3121 ttgtgccgta tcaattattt tgttgtatag cgtaaacgtg ctttcaaaga aatccttatt
     3181 cttacgatag atattcttat ccttagcgaa ctcagcaata ttcttacctt ctaagctagt
     3241 tgcattaagc aattccttct cttgtacata tccaagataa ttagataacg ttggtaccag
     3301 ttctaggaac tcacgacttg aattagtatt atgtttgatc ctgctaccga tcgtagcgta
     3361 tttagtttcc atcagtgttt caccagtctt aatatcaatc aactgttttg ctagtgcatc
     3421 aatatcttta ccaagcttca gcaaagcttc aaaaccatct ttagatggat cagaaatgcc
     3481 tacgacaaga tcagatacag cccaagcatt ttgagctagt agacctgcat cattatgagt
     3541 atcaatcaga attagatcat acttctcttt aagctgttca tagttaacaa ccatccatct
     3601 aaacagtagg gttctgttgt taggcatatc acgcatttgc atctcaactt gttttaaatc
     3661 ttcacctgct actaaaatat ccatgttctc atttacagct aaaggttcag ctgatccatg
     3721 aagatataaa tgattgattg tattctctct tttcgcttta ctgaaagcat caggaaatcg
     3781 ttttgatagg ttttgcgact tatcttcatc aatatacagc accttcatac ctaaattttc
     3841 gacagcatat ccacctagat tgtagattgt tgttgtttta cctgttccac ctttttcaat
     3901 gtttccagta atgattgaca acttcttttc catgattaaa cctcccttat taaccttttg
     3961 taactccatt atacactata aacacgttta tgtatatgtt tatatacata aacgtgttta
     4021 tatttatatt tgcttttata ttctacaaca taaacatata tatgtatatg tttatgttta
     4081 tgtatatatt catatatata actattgcat ttgtcataca atcgtagtac aatgtaattg
     4141 aaaaggagtt gataaactat gccagtaata agtattcgtg tatctaaaga agaaaaagat
     4201 tttcttgaaa aagttgctga attcaacgga gataacattt ctgaatttat gcgtagtaat
     4261 tccctgcaaa cagcagaaac gttagtagac tttgccacct acaaggaact aatgaaagag
     4321 catgaggaaa acgatcaaag tatttcccac gaagatatgc taaaagagtt aggcttataa
     4381 gatgacttac caagtaagat atgaacgtga agctcaaaaa gcgctaaaaa aaatggataa
     4441 gtttcaggca aaggttatcc taaactggat cgagaaaaat cttgtaggca ctgatgatcc
     4501 aagaaaacat ggtaaaggtc taacagctaa taaatcaggc tactggcgat atagggtagg
     4561 tgcctaccgt ataatagctg atattagcga gcatgaagtg acaattctta tattgtctat
     4621 tggtcacaga agtgaagttt ataagtaaca gacaagctga taaggcttgt ctgttttttt
     4681 gttaacccaa caaaatcact gtttacgatc ccactttacc taaaaaagaa caataccctc
     4741 ttgttattat acacactggt gtgtataata taagtgtaaa caagctaatt aactttcaaa
     4801 aggagcgatt ttttatggga tggtatggtg cttattcatc agcaaatgaa gtgatcgaag
     4861 aaaagaaagg gtacgccaaa aatggtttgg aaatcgtaga taccaaagta acgaagacct
     4921 atggtattat ccttttcaaa gatgcagaag gaacctacga tattgacagc ttcatctttc
     4981 gagatgaaat gtacaaacca atctacttct taaatgacag tcctgcttac tttaagaaaa
     5041 ttccgaaaaa atggttgaaa ttctttgtcg gtgatcaaga aaaaatggtt aagcaacaac
     5101 tagatagaat taaagaagaa aacaataaac caaaccttga tgacttatta gttgcaggta
     5161 aaacttactc aatctgggat ggatcgtatg tcgctgttta tcagtataaa ctcaaacgta
     5221 gctttatttt tagactacaa gatggatcac tcactcggtt cactcgacta tcagcaggag
     5281 atgttaagga agtagcctaa aacttaaata cataaacata aacgtgtata cgtttatgtt
     5341 tatgtttatg tatatgtata tgaatataaa cgtattaggt acgaacgttt ttaaatgctt
     5401 ttctgggctt tgattttagg gtagggctaa aacatccatt gatccaaaac agctcagaaa
     5461 cgcatttaaa cgtatctgat accaatatta cagaaggagt gtatcgtatg attaacctat
     5521 ttcttttagt gacagctagt acattattct ttactggaat atcactgata atttcctatt
     5581 tgatctataa gaaagccgaa acttacacaa gcaaagcaac cggactagcc gtttcctcct
     5641 ttttaacttt gctgactttt tttgttactc tatcgttttc ttttgtttag cacagctgat
     5701 ccatcagcaa aaaacctctc tttacattat acacactagt gtgtataatg taattgtaaa
     5761 caagctaatt aacttttagg aaggagtgac aatatgctaa cagctacatt acagcaacct
     5821 actcacgttg ctattgcaga aagctataca attaatgcag gggtcgttaa acagctgatc
     5881 aaggatcgag aattcaaaaa gtttaacagt cgtcaagcgt ataacagaca gttaaagaaa
     5941 gctattctaa agcacaaaaa ttccttcata gggaaattgg aaagtgtgga tgttaaaact
     6001 gtttcaacgg cattttcagt tgttgaagtg actattgacg gtcgcttgat ttctctattt
     6061 gcttttggaa ttaattgata gggagtgtgg actttggaag gtaagacttt tgcagaaatt
     6121 tggactgaat ttattgaggt accaactcgc attgatccga taacttctga aaaggtattg
     6181 gatgcagact tctacaatct caaagcaggt actagccttg aatgcgttta gatgtattta
     6241 gaagagcatt tcaaaggcgt aacgctcggt cacgtcttat accacaatgg tgccgagtgg
     6301 gatgcaattg gaaatatcca tttttcttaa tacaagaaag gagaacacaa tatgaattat
     6361 accgatgaag aacaaatcac tttggaatac gttttcacaa atgaaaaagg ggaggaaatt
     6421 gaaaaagtct tgatcatttc atcaaaagac ttggagaacg ctttgactta tgaaccatac
     6481 gaatcaaccg taactgtcaa tgaattctta gatacctata cttctaatga tacttgggaa
     6541 attatgaata tttgcaacgc taataacatt caatttgctg aaaaaaccaa taaatcattt
     6601 tattagtttt gcaataaaac atcctctttt tctattgatt ccatacacaa caatgtgtat
     6661 aatagatttt gtaaagggag ggtagctatg cctaagacag caagagaaat tgtaaaaatt
     6721 ctcaaatcta acggcttcaa aaagactgga caaactggca gtcatgcaaa attttataat
     6781 tccaagacta aaagaacggt cgttgttccg atccacaatg gtgatgtacc actaggaaca
     6841 gaagctaata tctggaagca agcagggctt taacagcctt gccttcctat ttggtattag
     6901 gcatactatc ctctcttaca ctagaaaagg agcgaggaaa tgaagacaaa aaatattttt
     6961 tatccggcaa tcttcgagaa agaaggagaa gcttataaca tcgttttccc tgacttaccg
     7021 gaaatatcta cctttggaaa tagtcttgaa gaagcatatt taaacgctaa ggatgcttta
     7081 ggattagcgc tatatagtgt accagatgca gaatttccta aaccatcagc agtggaagaa
     7141 ttaactttga aagccaatca agtagtagta atcatccaac taaacattaa acttttccga
     7201 agaagcttaa atacaaaaac aattagaaaa aatgttagtg ttccggaatg gttagttctt
     7261 ttagggaagg agaaaaacat aaacttctct caattacttc aaaaagcact agaagaagac
     7321 ttgcttgaaa agtaaaaagg agaccgaacc tatgaaatct acacacttaa actatttctc
     7381 tcgaacgtta atcaataatc tattaaaaga tacaactggc attagcgtta gtaccaaaca
     7441 ctttgccaat gatctaatta tttttgagga taataacttg cgaattgaga tgtttattga
     7501 acaggcaact cctaactcaa tacctgaatt ctgctatacg cacactgaaa aaatgaaaca
     7561 tgcaaaatca gttaaagatg ttgaagcatt aagtgaatca ctaacgaacg ctttaaacct
     7621 cgttaagaag gctaaattgt tatttgaatt tcaaaccgac taccacaatg agttgcttgc
     7681 tcaatctgaa attttagatt aattgaaaca ggagaagaga aagatgaaac aaacacaaac
     7741 agaacccatt aacgatacgc ttgctgatca attagcaggt tattataaat tatttgagat
     7801 gagtaaagca cagcgcttga tccactatct ttggaaatac aaaattttgt ttcttttttg
     7861 ggaagcaaca ctaacattct cagttctttt ttataagtca gatttttata atcaacactt
     7921 agttttaggg agttttttgc ttatctacac cattttgatg cttaatttat atacctacat
     7981 cacacttgaa gaacacttag taattagcga tgcagggatc gagagaaaca tcaaaagggt
     8041 atctttcaag atcacgttaa acaagcctac cgataaaaag ctctcagaag cgttctgaga
     8101 gcttttgaca ttttcttgta taatttatcc taacacatta caaagcacct aattcgagct
     8161 tatatcaagc aaatttgcta tttattcctc aatacatatc taaaaattac ttgatggaaa
     8221 actagataca tggtaagcat gaaaagaaaa tcaatttgag cagatgcagt ataatacata
     8281 gcaatttaag gctctatact ttttgatgtt ggtgaacttc accaacatct tttttgtata
     8341 aaaaaaatat agaaaagaca ccctgaaatc ctttagaatt aatttggcta ccaattcata
     8401 ggaggattca agatgtctca tactcagctt atcaaagata ccctaaatat tctagaccta
     8461 aatatctatt tcgaggaaaa ttgtttaact aaagaaaaat acaaagggca aatttgcttg
     8521 atttacaaag gaactcttct gtacaagcca gaagagtgtt cccattgtct ttgtgtcgtc
     8581 ccctctagga tcattcgttg ggggacgaca accgttcggt tgttactgaa tgatgtgtct
     8641 gagtatcgca cttatctgga gctgaagaaa caacgattta aatgtaagtc ttgccaaaga
     8701 acattcgtag cggatacctc tgttgctgaa aaacactgtt tcattagtca aaaagttcgt
     8761 tggtctgtca ttgctcgctt aaaagagaat acttccatga cggaaatcgc tcgtcagaaa
     8821 aatatttcca cttcttcggt ctatcgtgtc atgaagcgat tctaccgccc attgaatcct
     8881 ttcaaacaga ctttgccaaa ggtcttatgc tttgatgaat tcaaatcggt tcgaagtgtt
     8941 gccagtgcaa tgagttttat catgatggac ggacaaagcc atgcattatt ggatattgtc
     9001 gaaaatcgac gcttgcctta tttagaacgc tatttttctc ggttttcatt agcgactcgt
     9061 gaagccgttc aattgattgt tattgatatg tatgcgcctt atgtttcgtt ggtgaaaaag
     9121 cttttcccga atgctcagtt gatcatcgat cgtttccaca tcgtccaaca tattggcaga
     9181 actttcttga atcatcgagt gaaagaaacc acggtacgac ttaaaggaaa ttcccactct
     9241 caacgagggt tagggaaaaa attgaagcgc tattggaaac tcctacaaaa agaagaagca
     9301 aagttaaact atgaaaaacg ggtttggcgt gccagtttta aagattattt gactgaaagt
     9361 gagattgccg atcgactgtt agctgcttgt cccaacttac gccaaggcta tcagttatac
     9421 caagacattc tttatgcggt aaagaaaaga gatcagtcac tatttgagga ttgtttaact
     9481 agagacatta gcggtctgcc agaaacctat acaaccactt tacgaacgtt taaaaagttt
     9541 ctgccacaga tccaaaatgc gcttcactac agctattcaa acggaccatt ggaatgctta
     9601 aacaaccaca tcaaagtatt aaaacgcaat gcgtacggct ttcgcaactt ttacaatttc
     9661 aaactacgaa ttatgattcg tcatggcaaa acatttttaa ccaaataaac agaacagaaa
     9721 gaggttttct tctttctgtt ctgtcc
//
LOCUS       DACCXO010000101         9716 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.103, whole genome shotgun sequence.
ACCESSION   DACCXO010000101 DACCXO010000000
VERSION     DACCXO010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.103"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..180)
                     /locus_tag="IVY70_002254"
     CDS             complement(<1..180)
                     /locus_tag="IVY70_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959369.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTIS"
     gene            complement(196..810)
                     /locus_tag="IVY70_002255"
     CDS             complement(196..810)
                     /locus_tag="IVY70_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP7959370.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(957..1640)
                     /locus_tag="IVY70_002256"
     CDS             complement(957..1640)
                     /locus_tag="IVY70_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP7959371.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1749..2207)
                     /locus_tag="IVY70_002257"
     CDS             complement(1749..2207)
                     /locus_tag="IVY70_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP7959372.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2291..4054)
                     /locus_tag="IVY70_002258"
     CDS             complement(2291..4054)
                     /locus_tag="IVY70_002258"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP7959373.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4275..5735)
                     /locus_tag="IVY70_002259"
     CDS             complement(4275..5735)
                     /locus_tag="IVY70_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959374.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5943..6869)
                     /gene="glsA"
                     /locus_tag="IVY70_002260"
     CDS             complement(5943..6869)
                     /gene="glsA"
                     /locus_tag="IVY70_002260"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP7959375.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6892..8319)
                     /locus_tag="IVY70_002261"
     CDS             complement(6892..8319)
                     /locus_tag="IVY70_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7959376.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8350..9564)
                     /locus_tag="IVY70_002262"
     CDS             complement(8350..9564)
                     /locus_tag="IVY70_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP7959377.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2874 a         2029 c         1617 g         3196 t
ORIGIN      
        1 gctaatcgtt tgatctggca tttctatttc cttaccatct aaagtaacag tagtaacgac
       61 aaaatggtct tgcaatggcg gaacttctaa gtactgccct tgcagaaaat cattcactgt
      121 ttcatattct tttttcattt cttttgaatc tgtcgatggt ttatagctta tttttaacat
      181 aactagtgct cctttttatt cgggacgtgc aatcgtgaaa atttctccta ttgctgcata
      241 atcatttcct gccaaacggc ttacagcatt caattttctt ggatcgatcc ttcctgtctt
      301 atcatcatag actgtttcat ttaaatgata tttctgtact tctagcaaaa ataaatcagc
      361 tgttacttta tttttattcc gtatctcgac atgttcatat aaggatgttt caaaacgaac
      421 ttttgcttct gtcaatgctg gtactgaaat gattactgaa tcagttaaag tcaagtcagt
      481 caggtacagt tcgctttcct cattaggcag attggctgct gttttattta cttcttctac
      541 gttgtcctca tcaaggatat ggataacagc ttctcttgtc tcaagaatat tctttgctgt
      601 gtcttttact gcatcttgtt ttctctggat cgccaaggat aagatgggtc gattcgaact
      661 gacgatattg aagtaactga agggtgcgat attcactata tcttttgctg atttagtcgt
      721 tactactgct actggtctag ggatgactga accaatcagc aacttgtaat tttcacgttc
      781 tgtcaattct gaaggactcc gttcaaacat ggtttcgcct tctttctata aaattcgtta
      841 aaaagcagat cttgaagaca aaatgaaaag taatgctttt ttcatcttct tagaatctgc
      901 tttaaactat ctatcaattt ttcttttttg tcaagaatct gtgccgtgcg tgttttttag
      961 ttatttgctt tacttgcggc ttcgacatat cttttagtga tcaattgcgg acgggtgata
     1021 atagatccaa ctacgactga aaaagcacct aattcatatg ctttctctac ttgttcagga
     1081 gagtcagtat gtccttccat aattactggg atatcgactt gttctatgat ttgattcaat
     1141 aaatcaaaat caggtgtctg cgtctttcgt gaatattctg tatagcctgc taaagtcgta
     1201 gacaaaagat caaaaccatc ttcttgcgct tgaagcgctt cctcaaaagt cgaaatatct
     1261 gccatagcta accgaccgtg ttcgtgaatc atttggacaa gttcagcagt tgttttttct
     1321 gtcggacgtt tacgagaagt tgcatccagt gcaatgattt ctgcttgcgt ctccaaaagt
     1381 tcttccactt ctttttctgt cgcagtaata aacacttctg acccatcgta atctcttttg
     1441 ataatcccta ttaccggtaa atcaacgatt cgcttgattt cattgatatc ttctgtcgta
     1501 tttgctcgaa taccaactgc tccgccttct tttgcagcca aagccattct ccccataata
     1561 aaagaactgt gtagcggctc gttttccaaa gcttgacaag aaacgacgac tccgcctttg
     1621 atttgttcca gtgtatccac aatatctcct ccagtaatgt tatataaaaa tacagcttca
     1681 tataaaaatg taaccctttt ctattcagga gacaataatg agctatttct ttcagcagac
     1741 ctttcttact aaaagaaaat tacttcaaat tcttcgtata cgaccagctt ctcttctgta
     1801 aattctatgt tcaacgcttc tttataagca tgtttaaaaa actcagcatg gactttccgc
     1861 caataagcta atgtccgatc cccttctcct tctttccaag catgctcttt cgtgacttca
     1921 ctaaaaggca agatggtcac atttatattt ttgatgatac aaactggttc attttttcca
     1981 ttcaggacca cgtctaattg accaactttt ggtaaaggct cattctcttg tttatacaga
     2041 tcgtaaccac ttgatgtacc agtctttatc ttttttacaa ctagttcgct caactcgtct
     2101 gccatttttt ctgtatcccc aaatgaaaag aaacctaatt tttctgattg ataactagga
     2161 tatttttctt ggaacttttt gacatattct tctacttgct gatacataac atccacccct
     2221 ttgcttttta taatgaaaaa accatccaga tactagtata aacaattatc tggatggtga
     2281 gcataatgaa ttattgaata ttcaagacac gcagactatc ttcaacgatt tgttttggtg
     2341 ttaaatgata acgttcataa agctgcccta caggaacgct atccgtgaat tctttctctg
     2401 caccaaaatt taatacgttc attgaagaat caccataata acgactgata ttttcgccaa
     2461 atcctcctga caaactacca tcttctaatg tgatgactaa ttcgtgtgtt tcttttaatt
     2521 tatctaatgt ttctacgtca ttttctgtga tcgttcctgg attgatcaac gttgcttcga
     2581 ttcctttttt tctaaaagct tttactgttt cttttcctaa ttcaagaaaa ccgcctaaag
     2641 ctaaaattgc tacttttcct ccattattta ctgtttgata ggctggaata ctataatcat
     2701 ttgcgatagc tgtaccatgt tctacaccat gtgtaggaat tcggattgct acaggctgcg
     2761 ctgtttgttc gaacgcccaa tctagcatag agatcatatc ctcttttgtt gctggagcaa
     2821 gatatttgat gttcgggata cttgataagt aaggaatgtc aaatgttcct tggtgtgttc
     2881 ggtcactagc tgagatcgtt ccgccaccga ctaaaataat cactggattc tcgttgatcg
     2941 ctaaatcatg tgataattga tcatatgccc gttgcaagaa agtacttgat tggaagacga
     3001 ctggacgtac tccctgagaa gccaaagcag aagcgaaagt gatagagtgc tgttcagcga
     3061 tccccacgtc aaaataatga tctggatgtt tttcttggaa tgttttcagt ccaaaggcgc
     3121 ctggtatcgc tgcattgact gcggcaatag gagctgtact tgtttgcatt ttcttatcca
     3181 aataatccat gatgatcata ttaaatgttt ctccagaagt tacttgcttt gcctccccgg
     3241 tggacagatc aaatggtaca tgccaatgat atttcatttt attatcgatt gccggctgat
     3301 aaccgcgacc tttttccgta tggacgtgaa gtacaatcgg atgatcaata tcttttactt
     3361 cttcaaataa acggatcacc gtttccagat cgtttccatt atccacgtaa cgataatcaa
     3421 atcccattgc tttaaatagg ttattgctgc ttttaccgtt ggtttgacga agctcagcta
     3481 agtttttata taaactgcca tgattctcat caatagacat ctcattatcg tttactaata
     3541 cgattagatt ggattgtaaa acaccaccat tgttcaatcc ttcaaaagct aagccaccac
     3601 ttagggagcc gtcaccgata aatgctacca catttcctga ttctttcttc agatctctcg
     3661 ctttagctaa acctgttgct aagctgatag atgtagatgt gtgccccact gtaaaatagt
     3721 catgtttact ttcatgttgg ttggaatatc cgctcacttc acggtaatgt cctttttcaa
     3781 aaccattttt tcgaccagtt aaaagcttat gaccataaga ctgatgggaa acatcccaaa
     3841 cgattttatc ctttggtgaa tcaaatacat aatgaaacgc aatcgttaat tctactacac
     3901 ctagattcgg accaacgtgc ccacctacat cgctgtcctt ttgcaagatt ttttggcgca
     3961 tctcttctgc caactgtttc atttcctcaa cagataattt tttgacatct gcggggctat
     4021 tgattgcatc caatacatgt aatactgtac tcataataca ttctctcctc cgtactatct
     4081 tcttttaatg aaaatagatt tgttacactt tatctattct actacttaga gttgactcta
     4141 agtcaatagc attttaaaaa aatttcagag aaagcttgtt atataagtag atcttcgcac
     4201 gaatcaggac tcaaataaaa accttctaca gacaattaaa aagctttgta gaaggtctcc
     4261 taattaaggt ctcatcaaga aatatgtttt tttaataggt gaagttcctg agcaatatga
     4321 tacaaatgga tgtttgcacc ttttaatgag attggataga tctttgtctt ttcagtttct
     4381 aatccatttt gatagctagc agcatcgcac aaaaccactc tttttttcgc acatagacat
     4441 tcatcaggca attcttctaa tgaacgaaat aaaataggat ttatttcagc gatgccttct
     4501 tccgcataca aatgaaattg ggttcgataa aaaaattgtt tgactgaact ttctgaaaca
     4561 acaaaaattt ctatttcctg tctccccgct tgttcactgt tgatcaaaga aagacttttg
     4621 acgaattttc gaatgaaatt ttcattccat cttagttgga tttgattttt ctttttccac
     4681 tcttccaata tttctacaat ttttccgtac agaagttgtt ctttttctga tagaagatag
     4741 atcctttccg gtaaaaatac ttgtccatct gcccacaacg tacggacaaa ttgtaaaaag
     4801 gattgcttga aaataaaatt ttctgcatcg tcgatttcag cgatactaat aatctgtaaa
     4861 atcaattcat tcagtgatag gtcattgaga acaaaatttt tataaacaac ttcgaaatct
     4921 tttcttaaca gctccatgtt ctcattggta taatttctac tgttgaacaa agaaaaaata
     4981 taatagacat cttcttcttg taagtctaga attccttttt gctcttccaa tccttgacag
     5041 actaattgaa ataacggctt cgtttctgcg cttttcttat cttcttcttt cagtccaagg
     5101 ggaaatgcct ttcttccgat tgcgatctct attcctcgta tgataaagga cttttcatct
     5161 tccgaatatc ttcttttaaa aaaatgagtc tctacataat caatcaactc gttacaagct
     5221 tcttctacat acggattttt attttcatag ccttcccaat tgacataacg tactaaagct
     5281 agcaagatgt tccgcgagtc aaattctggg ataatgattt ctttattttt caaataatca
     5341 ttttctttaa aaaaatccaa tacgattttt ttgattgtat atactttgct caaagaaata
     5401 aattcaagat cagccagttc tgtactcgtg aatataccat taaaataata acttaaaaac
     5461 tttaaaaaat aagattgacc gtacagaaac tgtgtgagct cagaaagagc aataccatgt
     5521 ttgaatacga gtttatactt atgcttctct cttacttgtt cttttataca atattctaga
     5581 cactcggcta gactttctag atcgtgaagg atcgttggtg tagtcaccct tagagaaata
     5641 gctgtttgtt cgatgtctat ttcttggaat tcaaataatt gttcacacag atacactttt
     5701 ctaaatatat ttcgctcgat gtaattttcc agcattcctg atacacgcct ccagtttttc
     5761 ttatgatttt atttaatcat atctgtaaat cgtatttttg tttccttttt atttagaaaa
     5821 gccctttcaa aaaccaataa aaaaagaaac tgggacaaaa gtcaaacaag ctcacaattt
     5881 cagcctcatt gtaggtagct ttgagccgtt tggactttta ccccagcctc tttcaagaat
     5941 ggttattcaa agatgtcagc gtctagttct ttgactacat ctttcaacaa ttggatacca
     6001 gctgcgctgt taccaaatgg atctagtgcc gggctgaaga cacctatgcc gtaacgattt
     6061 ggcacagctg ccatcaaccc gccgcctaca ccgctttttg caggtacacc aacatggacg
     6121 gaaaactctc ctgattcatc atacaaacca gcagttgtca taattgattt gacgattgta
     6181 gcactttctt cagtaatgat ttgtttgcca ttccaaggag caattccttt atttgccagc
     6241 acagcagcta attttgctat gcctgtagcg gtgacattga gggagcattg tctgaaataa
     6301 gcatcgagta tctccggtac gtctccttcg atcatttgat tacctttcat atagtaagca
     6361 agagagcgat tgatatcacc tgttcttgat tcagaatgat agatttcttc attcaatgtg
     6421 atattaggat catcacagat ctctttcata aatgacaaga ttcgctcaaa tttttcatct
     6481 gcatctttac cggcaatcaa cgaggttgtt gcaatcgctc ctgcattaac aaaaggattc
     6541 attggatgtt tgcgatgatt gatttccata ttcatgatag aattgaaggc aaaaccggtt
     6601 ggttccgtgt tgattgtttg aaagactttt tctattccgt tgtctttgat tgctaataac
     6661 aatacgggaa cttttgacat gctttcgatg gcaaagcgca catcggcgtc tcctccctca
     6721 atcagttgtt ttgtatccaa atcataaatc gccataccta attgtttggg atccacttca
     6781 gctaatgctg gaatgtaatc cgccacttgt cctgtttgat aatagtggaa attttttcca
     6841 atcaattgtt ctaattcttt tacttccatt tgaataacca gctttcattt tttatttttc
     6901 tgcatgctgg aatttatgac ggcatgcgta gatcgtaaat ggaataacta agacgataag
     6961 ataagaaaca atcagaatcg tttcataaat cccatgctgt ccagctgcga tactagcagg
     7021 tggaacaaat gagatacaaa aagcaaaaag tgaagtcaaa agacctaaac aagcaacgat
     7081 tacttttaca acttttccgc ctggaacctg ataggctctt tcaagactgt catctttaaa
     7141 aaccagcttc aaatagcccg cgaaaaacat aaagtacaac atcaggtaga ttacaacagt
     7201 caatgacatc gctgcaagaa atgaaagatt attccccccg ccgcttaatg taaagacggc
     7261 tgcccagatt gttgcaacga tcccatttgc gatcatcaaa tgagcaggaa caccgtgtgt
     7321 gttttctttt gcaaaatatt caggaagtaa tccttcttct gctgctacgt gaagtgcttt
     7381 tgaaggagag gttacccaag aagcaatttc tccgatgaca cctaaacaaa caagaatcgc
     7441 aagaattttc gcaatccatg caagatttgg atcaatgtgt cttaatagga attctaacga
     7501 ttgaatcacc cctgtattaa gagacaaacc atgttgaggt actgtagtag cgacggtcaa
     7561 tccgccaaaa gtatctaaaa tgattgccag aataaccaat acaaacattg ctaatggata
     7621 atccttttta gggttttcca tttcatttgc atgactggca gatgtttcta cgcccatata
     7681 tgctaaaata aaggaaacaa aaacaaccag cgtaggcaat ttcgaaaaat caggaatcaa
     7741 tgttttcata gacagtatcg tttgtagtgg agcgcctgaa gcgacataat ggatccctaa
     7801 gccaaacaag actaatgctg gcaacaagat accagctaca aaaccgatcc gagcaatcaa
     7861 tgaagtgtat ttggttcctt ttaactgtga gagtaaaata gcccagaaaa taatcaatag
     7921 gattgccaat ttcagatatt tattgttttg tatagcacca atacccgttg cataggataa
     7981 tgcaccagtt aagaaataca gcatggtcaa gtagccaact gttatctcaa accattggaa
     8041 aaagattgcg gcaaaaccga atcgttttcc caatgtgcga ctgacccaag tatagacacc
     8101 gcctttttcc catcctttca ccgttgccat ctctgctgcg cacaatgcca cagggataaa
     8161 ccatagcaaa ccgcctaata acaagaaaaa tactaagctg aagcctgagg tcgcgaatgt
     8221 gggatattca tataatgaaa cgaccattga gatggtcata gccaaaaagc caaagaacgt
     8281 taattttttt gcaggaattt tcgttttgct ttgttgcatg aatcttcctc ttttctattt
     8341 gaatttgttt caattgagtt tgttgccaaa gaagaaaaag tccaatcctt cttcttgttc
     8401 ttcttttgta gtagtgatcg ttgtaaagtg catcgccgat tttaaaatca ctgttgtcag
     8461 cacataggca tacacggcag taatgatgac accaatcact tgaatcccta agaagtacca
     8521 gctgcttgga ttcgttagta aatggctgtt agatgcaaag atccctatca gaatcgtacc
     8581 tgtgaatccg cccatgccat gaacacccca cacatcaagt gcatcatccc attgtttctt
     8641 tttacgaaaa tcaacagcag catgacagac gatacccgca atgatcccaa tcagcatggc
     8701 actttttgct tctacataac cggcacaagg cgtaatggct gccaatccag caacggatcc
     8761 tgttagtacg tcgacaaaat caaagccatt tccttttact tttgcaatga tcagccaagt
     8821 aatcatcgca aatgcaagag cgatgaatgt tgaaccaaat gcattgactg cttgttcatc
     8881 tgctcgaagt gcaccaccgg aattaaaacc gaaccagcca aaccaaagca gtcctgtacc
     8941 gatagcagct gccataagac tgctatgctg acttctttct cctaaaggaa tctttctttg
     9001 accaagtgtg aggatacatg ctaaagagcc gaatccagct gtcgtgtgga tgactgctcc
     9061 accagcgaaa tctctaaatc ccatctgatc caaaaatccg ccaccccata tccagtggca
     9121 aacagggaaa taaatcagca gattccaaaa aataacaagt ataatatagc cttttaaatt
     9181 caatctccct gcaaaagctc ctgtcatcaa aggaacggta atcacgataa acatcagttg
     9241 gtaaagaaag aacattaaaa atggaatcgt tgcaccatga aacatgttgg ggaagcttgt
     9301 aacatgtctc atcaaaaaga aatctgccgg gtttccaata acaccgccga tatcccgtcc
     9361 aaaggccaat ccgaatccac caaataccca cataatcgtt gttatcccaa cggagagaaa
     9421 agattgagac atgatcgtta gtatattatt cttccgtgct aatccgccgt aaaaaaaagc
     9481 tagaccagga gtcatcaggc aaaccattgc tgtacataaa atcatgaatg ctgtatctcc
     9541 tgtattgata ggtaatgtat tcattttttt cgcctccttt ctacgattac gagtataaaa
     9601 gaaggtgtag ttgttttttt cttctgtttt tttgaattaa tacttgcatt tataaaagac
     9661 agacaattaa taaaagcttg atgtgttgtg ctctttttcg tttttcacac aaataa
//
LOCUS       DACCXO010000102         9667 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.104, whole genome shotgun sequence.
ACCESSION   DACCXO010000102 DACCXO010000000
VERSION     DACCXO010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.104"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..650)
                     /locus_tag="IVY70_002263"
     CDS             complement(<1..650)
                     /locus_tag="IVY70_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7959378.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIG"
     gene            975..1391
                     /locus_tag="IVY70_002264"
     CDS             975..1391
                     /locus_tag="IVY70_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7959379.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1388..1858
                     /locus_tag="IVY70_002265"
     CDS             1388..1858
                     /locus_tag="IVY70_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7959380.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            1872..2645
                     /locus_tag="IVY70_002266"
     CDS             1872..2645
                     /locus_tag="IVY70_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7959381.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            2659..3471
                     /locus_tag="IVY70_002267"
     CDS             2659..3471
                     /locus_tag="IVY70_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7959382.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            3525..4490
                     /locus_tag="IVY70_002268"
     CDS             3525..4490
                     /locus_tag="IVY70_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7959383.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            4606..7329
                     /locus_tag="IVY70_002269"
     CDS             4606..7329
                     /locus_tag="IVY70_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7959384.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            7342..7974
                     /locus_tag="IVY70_002270"
     CDS             7342..7974
                     /locus_tag="IVY70_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7959385.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            8873..>9667
                     /locus_tag="IVY70_002271"
     CDS             8873..>9667
                     /locus_tag="IVY70_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7959386.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLYL"
BASE COUNT         3261 a         1477 c         1884 g         3045 t
ORIGIN      
        1 ccaatcatat ggaagccacc gattaggtat tttatgggtt tagtcggaaa tgtttcaaga
       61 gcaatttcca tcatatcgat aatgccatga tgactacagc cagtaaagac aatcagaccg
      121 tcttcttgct caattacgac caataattca tctgaaaaat catcataaac aaatttatca
      181 tccatctttt tgtataaatt cttggtggtc gagtttgctt tgcctagggg gatgatatgg
      241 aagctcggag caagttctgt tttcgttttt attgtatgta aattctcatg cattttaagg
      301 atttcatttt taggaatacc aacatacaga aacgtggtta aaatctttaa ataatggggt
      361 aaagttacac cttcagctgt ataaataggg gcgtttcctg attttaaata tcttaacccg
      421 ccggtgtggt cagaatgacc gtgtgaaata atcactgcct ccagatcatt caaagtttcc
      481 cccattttag tcgcattata gactaatttt ttattagcgc cagcatcgaa gagatatttc
      541 ttccctgagt agttaattaa gagagataac ccatgtccac ttttaaagtt caaatttatt
      601 cttgagtttt caaataaaac cttgatttcc actataacat ctcctttcac atgaacgtgt
      661 attcatatat tgtagcacat taagttctat tttttgttgt aattaccgaa aaaaatatta
      721 atggacgctt tttacgcttt atttcgtatg tggaagaaaa tttcttagga tgtggcgggg
      781 gattggggca aagctaaatt ccaattacag tgatcattta attagacacc tttaaatacc
      841 cacactttca tcaacactat aatttaggga aagaaaagtg ttggcatgat atagaaaaac
      901 ctttaattaa cagcgtttcc taaaattggc acgcatcttg cttatagaaa agcgtaagga
      961 taggaggaat gaaaatggca actataattc ttgccagcca tggggaattt gctcaaggat
     1021 taaagcaaac agctacaatg attattggag atagtgtacc aattcatgcc ttgtcagcat
     1081 ttagagatga agatgaatct attttggtac aaataaaaaa acttttagcc acaatagata
     1141 tagaagaaac ctacatttta actgatattc taggtggtag tgtgaataat gatatgctca
     1201 ctattattaa agagtatgag ggattgcaat taattactgg aatgaactta cctttagtta
     1261 tttctattgc aacctataat ggaaaaattg gggaaagtga gctagaaaag cttattaaag
     1321 agagtcaaca atcagtgatt gattgtagaa aattattagt agaacaactt actggaggag
     1381 atgacctatg attaagttag tacgtattga ccatcgtttg ttgcacggac aggtagtatt
     1441 ttcttggagt aaatcattaa atatcaatcg tattattatt gccaataaag aggcagcgaa
     1501 tgatgaattc aaaaaaatgt ctttgaattt atctaaaccg actggcatta aattaacaat
     1561 tttttcagtt gaacagttac tagaaaaaat gcctaaaatc gaaacactta acgaaaatat
     1621 catgcttatt tttggtaata caaaagaaat tcgtcaattt tgtgaagcct atcctaaagt
     1681 acaagaaatc aactatggag ggataatcaa aaaagaaggt tccaagcaat ttagtaatgc
     1741 cattttcttg aatgaatctg agcaagaaga tgccaaaaaa ttaaaagctc ttggcattaa
     1801 acaatttatt caacaggtac caacttctaa aaaagaagat ttgaatacaa tgttataaag
     1861 gaggaatgaa aatgctcgtt cagtcaattg gtttagggtt aatcggtgtt ttttgtattt
     1921 tagactcaag gcttttagga agaatgaatt ttgaacgacc gttgattgtc agtacgcttg
     1981 ttggtttggt acttggagac atggaaaaag gattaattgt tggagcctct ttggaattga
     2041 tgtccttagg attagtaaat atcggagctg ctgcaccacc tgatatgaat atggcatcaa
     2101 ttattgctac agcctttgcc attttatcaa atgcgaatgc agaagcagca ttaacgattg
     2161 ccattccaat ttcagtgtta ggtcaaatac taggtatctt aatgcggacg attttgtcaa
     2221 atttgactca cgttgcggat cgctttatcg aacaagggaa atttagccaa gcaaggagaa
     2281 ttcatatact ttatggctcg ggattatatg ctctcatgta ttttattcct atttttctag
     2341 caatttattt tggaacgaat cttgtttctc agattgttga tgcgattcct gagtggctaa
     2401 caaatggctt aacggttgca agtaaaattt taccagcata tgggtttgcg ttgttattac
     2461 aaacgatgtt aacgaaaaaa atggtgccat ttttattact tggattttta attactgctt
     2521 attctggatt gggaatcatt ggtattgcag ccttcgcatg tattgttgcg tttgtcatgg
     2581 ttcaagttca aggtaaaaac tcaaatggta accaaaatga gccagatcca ttagatgact
     2641 tataaggagg tagattaagt gaaagagaaa gatcaaaatg catggaaata tcggcagttc
     2701 ttatggcgtt catgggcaat tcaagcctca tggaactatg aacgacaaat gaacatgggt
     2761 tttatgtacg gaattgcacc aattctagat aacatttaca agaatccaga agatgaagca
     2821 atgaagaaag aagcttacga gcggcatatg atgtactaca actgtacccc tcaaacaagt
     2881 gcatttgtgt tagggttaac tgcttctatg gaggagcaat actacgaaga ccaagaaaac
     2941 tttaatccgg aatcaatcag tgcagtgaag acttctctga tgggaccgct atctggtgtt
     3001 ggtgattctt tctttcaagg aacgattcgg gtattagcct ttggactagg aattaattta
     3061 gcccaacaag gtagtatttt aggtccaatt ctagcaatgt taatttcctt tgtaccttcc
     3121 tttttagtta cttactatgg tgggaaaatt ggctataaca tggggaataa atacttgtct
     3181 aaactgtatc aagaaggttt gatggataaa gtaatgtatg tttgctccat tgtggggttg
     3241 atggtcattg gttcgatgat tgccagcatg atcgggatta caacaccaat tcaaatgggt
     3301 aaagcttttg tcttacaaga tgtattagat agtattatgc cacaattttt accattagga
     3361 ttaaccttct tcatgtactg gttattgcag aaaaaggtca aaacaggatg gatgttggca
     3421 atctgtttag tcggaggaat tatttttagt ttgttaggta tcttacaata agggaataac
     3481 caataaattt ggttgaataa tataaataag gaggaattca attgatgaat tcaaaagaaa
     3541 ttgttaaaga aatcgtagcg aaaaaagagg taaaatcaat cgtatttgtt ggctgtggcg
     3601 cttcaaaagc ggacctttat cctgcaaagt attttctaga ccaaaatgcg aagcagttac
     3661 ggattagtca ttacacagcc agcgaattta actatgcaac accagcatct gtagatgagt
     3721 caactgtcgt tatttcagct tctttaggag gtacgactcc agaaacagtt gaagctaacg
     3781 cagtagctaa agaaaaaggt gctacagtta ttagtctaac acgaagcatt gactcaccgt
     3841 taacaaaaga tgcagattat gtaatttatc atcgatttgc agagaactat gctgctaaat
     3901 tagaaaaaac aggctactgt ttagaattgg cagtagaact tttacatcaa gtagaaggtt
     3961 atgaccatta tgaagcaatg caagaaggat ttgctactat ctatgatctt gcccaaaaaa
     4021 gtgcagaatc agctcgtaaa gatgcgaaga aatttgctga acgctttaaa gatgaaaaag
     4081 ttatttatgt aatgagtagt ggtgctacac atgaagtagc atactcaaca tcaatttgct
     4141 tgatgatgga aatgcaatgg attaattctg gtaccttcca ttcaggagaa tttttccacg
     4201 gaccattcga aattgtggat aaagacgtac cgtttatatt attaatgaat gatggaaaaa
     4261 ctagaaatat agatgctcga gcattaactt tccttgaacg ctttgatacg aaaacagaag
     4321 ttgtagatgc cttggattat ggcttgtctt cacatattgc taaagaagtc ttagactact
     4381 ttaacccatt cgtgattact gcagtattta gagtttatgc agaagaattg tcagaagcaa
     4441 gagaacatcc attaactaag agacgttata tgtggaaact aacttattaa aaaatagatc
     4501 aaactaaata aggtggcttg agaaattaag ccaccttgtt ttcttcaatg tgattttata
     4561 catatggtaa aataactagt acatattgtg aaagcaggtg gcaagatgca gcgattaaat
     4621 gactatcttt atcaactaac attgcttttg aaatctacgc cttcagataa ttattcagct
     4681 cgcagtattt cgcaagcttt acagttgaat agaagtacaa tcagtagcta cttaaatgaa
     4741 ggtgttcgtg aagggctttt cataaaagta aaaagttacc cggttttatt tttacataga
     4801 acagctcttg aagaactaca tatcacactg aataactccg aaatagaatc aattgaaagc
     4861 ttgttaacaa tttctcaaaa acccgcatta gatcaagtaa ttggctctaa aggaagtttg
     4921 aaagaagcaa ttgatcaaat taaaacggca gtcttatatc ctggaaaagg gttgccttta
     4981 ttattgatcg gcgctagtgg atcagggaaa acctttctag cgaataaaat atatgaatat
     5041 gcagtagaag aaaaggtcat tcacacatcg gcacctttca tagattataa ttgtgcacaa
     5101 tatgtgagca atccagagtt actatcctct gctttatttg gctacacgaa aggagcattt
     5161 accggagcaa gccaggagca tactggtttg ctagaaaaag ccgatggagg agtattgttt
     5221 ttagatgaag tgcatcgctt atctgaagaa gggcaagaga aattatttac atttatggat
     5281 acgggtgaat tctctcctat gggggataat agtattcgga aaaaagccga tgttcgatta
     5341 gtctttgcaa caacagaaaa tatatatacg actttcttac caacttttct taggagattg
     5401 ccagttattg ttaatctacc tagatttcaa caacgtccgt cgtttgaacg tctgtcattg
     5461 attgatgaat tttttgtttc tgagagtaaa attttagcta aggaactatc tgtttctgat
     5521 gcattgattc atttcttaat gaatagtgat tttgaaggta acgtggggaa tattaaaaat
     5581 attatcaagt atgcttgcgg aaacgcatat atccaccaga agagtcagca aacaattcaa
     5641 gttaaattat tggatatgcc tttagagtac agttataaat tcaaggagca actaaataaa
     5701 cccagaaaga aaagagcaga tcgaatctac tatccagaag aaacacgaca agtgcaacta
     5761 gaaagaaaca atcagctttt tgttcaattt tttgaccaat tacttactgg attctctgaa
     5821 gtgattgaaa ataacaagga tattaaacta tttttagaag aaatggttac caaggtaaca
     5881 caagtaatgg atacgcttat ttttcaagag gattacgaaa aagagcagtc tttatacgcc
     5941 attctaaact atcatattcg tcaaagtatt gattttatgc gagaaagcta cgggtttgaa
     6001 caagatggta accgggtagt cgctttggcc aactatctat atgtcaagga taatcaagtg
     6061 atttttgaaa atagtcttca agaaaagaaa accaaagatc agcttaacga attttttgct
     6121 aatgttcttg aaacccctta ttggtatgca aaaaaaatct taagcctttt atctgagtgt
     6181 ttagatcaac ctgtgtatga agaggacatt ttctttatta cgctatattt ttatagtttg
     6241 aatgtcacga atgttaaaac aggtattaag agtattgtgc tagctcatgg ttattctaca
     6301 gctagtagta tggcgaatgt tgccaaccgt atgctgaata agaacgtttt tcaagcttat
     6361 gatatgcctt tagatattac attagagaaa attgaagcaa aattgattca attccttgga
     6421 gattatcgaa cagattcagg cttgatttta ttggtagata tgggttcact aaaccaacta
     6481 ggcggtttat tagtgaatca tctaaatgga ccactgttaa tcattgatca tatcaataca
     6541 ccgttattat tggaagtcgg ccaacaagta ttaaacaata aaagtatcac cgaaatagga
     6601 gcagaaattg aaacaagtct tactttcaaa aagcagttac tacttcccaa gaaaaagaaa
     6661 aagaaagcca tcattacttg ttgttataca gggattggta gtgcgaccca gattcaggag
     6721 attttaacga aatgcttgaa agatgcagtt agagaattag taattattcc gtatgattac
     6781 aaaaagctgt tagaaaacaa gaattttgag actccttttc aattatacga tgtcatcatg
     6841 attgtaggta cagaagatcc caaaattaat cagattccat atattggatt agatcgcctg
     6901 attaatgggg aagcaattgg cgaatttgtt cattacttgg aaaaagagct gactattgaa
     6961 gtgaacgatt tacaaaaaga attaattttc aatttttcaa ttagtaaaat tgtcgaaaat
     7021 ttaaccattt tggatccaga taaggtgcta tcttttgtac aaaaagcaat taaagaatta
     7081 tcgaagttat taaattttga tttttctaat aatcagctgt ttttgttgta ccttcactgt
     7141 agttgtatga ttgaacgaat tgtacgtaaa gaaagtgtgg atgagcaaga ggacattgaa
     7201 gaatatatgc aagcaaatgg agaaataatg gataaaattc atagcgcttt ttttgagatt
     7261 gaaagggaat acacaattga aataccagat ttagagttgc gattattgag cgatattgtt
     7321 caaggatagg agtgaattcg aatgacaatg ggagtaattt ttgatatgga tggggtactc
     7381 atagacagcg agacatttta ttttgagaga agaatgaact tctttcaaga actggagata
     7441 gatccaggat caaaccagat tgatgattat gtggggaaaa cagaagctgc aatatgggaa
     7501 acattagttc ctgaggacaa atcattaaga gaggaacttt atccgaaata tgtagattat
     7561 cgaaaaacgc atccaattga tttcgctaaa gatttacgaa aagaagtgaa accgttactt
     7621 gacttcctca agaagcggga tataccattg gcactggcat cttcttctcc agttgttgag
     7681 atagagagta tgctcattca atgtgaactt actgaatatt tttcctttgt tataagtggt
     7741 gagtcattaa aacaaagcaa accacatccc gaaatatatt taaaagcgaa acagcaatta
     7801 gactgtgact tctttattgc agtagaggat tcaacgattg gtattaaatc agcaaaggca
     7861 gcaaatatct atacggtggc actaaaacaa ccaatgcaaa tcgatcagag tgaagcagac
     7921 atacaaattg aaagtctaca agaactggaa caaattgttt tagaaaaagg ttagtgaatt
     7981 aatggaaata aggctatata tctgtgctag ctattttgac taaccaaaaa aacttgctat
     8041 aattcaggtg tagtaaaagg tgctggtaac accttttacg aaaatgcttc ggcagtgata
     8101 atgggattgg ctttttaagt tttttatttt gttcgcaatc cctaaggtca tgtgaatgat
     8161 cctagggatt gctagaatat tttgcaacat tctatctatt tcagaacgta accttcccca
     8221 gttcattggg attcattagc atacaaccaa catttcaaaa agaaattgca ctacaccatt
     8281 attagctatt ttgactaacc ccaaaaaact tgctataatt caggtgtagg taaaaggtgc
     8341 tggtatcacc ttttacgaaa ctgcttcggc agtggtaatg agtgtggctt aaaggactag
     8401 tcctttaagc tttttacttt gttcgcaatc tctaagatca agttaatgat cgataagatt
     8461 gctagaatat tttgcaacat tctatttcag aacgtaacct ttcccattcc actgtgattc
     8521 attggcaacc ctcctcacaa ccaacgtttc aaaagaaatt gtaaagttgt tggggtacca
     8581 cgaccgaaca atactacacc tgaatgatag taagttgtct cattaagtat agcatatcct
     8641 ctttttgggt aaatagaaat cggttacatt ttttatccaa gaccaagttt tttcatttgg
     8701 tcttatttgc tatacccaaa caattccgca gtatagtcga cggtattgat tttatcaggt
     8761 gagtaataga aacttgtttt ccttccccaa aacattacct ataaaggaga aaaaatggta
     8821 tcgtaattat ataggtatag gatgaaattt ttttctaaga ggagggttgt tgatgaaaaa
     8881 tttacagtta aaatttattg ttgatccatc gattgtacgc tggtttcgga ttatcaatca
     8941 atttgagcgc aatcaacttg ccacaaaagg agaattagca aagagtaatc atgtatctga
     9001 acgaaccatt caaacagatg tcaataaaat gaaggactac ttttctgaaa gtgttcaatt
     9061 tgtttcaacg aaaagtgggt attcttttca gaaggaaaga ccggttcttt ttctcaagca
     9121 gaaggagcaa ctactggaga acgaaatttt atttgagttg ttagggaatg tcttttatgg
     9181 cgaattggaa gatatgacgg agctaattga tcgttttcat ttatctgaag cgacttttcg
     9241 tcggtatttg aagcagattc aaccagtttt ggaagaatac ggcttagaat tgagtcttta
     9301 tcctcttgat ttaaaaggag aagaggcaaa tattcgcaag ttttttaaag acttctatta
     9361 tgaaggggag atgacgcccc atactcttgt tccaccaagg gatcttcatg aacttgtgca
     9421 agctacattc tatgataaat ttgagtattt ctcgattgga acagggactt ctcctgctga
     9481 attttattat agtttgtata ttgcgattga gcgtgtgaga caaggaaaaa cgatagccat
     9541 tcctcaaggt ctgattgagc gagtaattgc tagtgagaat tttcgcttaa tgtattcctt
     9601 gaaagaagcg attcaaacaa attatcaagt tgctttatca gaagaggagt tttgttggtt
     9661 gtattta
//
LOCUS       DACCXO010000103         9455 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.105, whole genome shotgun sequence.
ACCESSION   DACCXO010000103 DACCXO010000000
VERSION     DACCXO010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.105"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            41..1015
                     /locus_tag="IVY70_002272"
     CDS             41..1015
                     /locus_tag="IVY70_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7959387.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            997..1458
                     /locus_tag="IVY70_002273"
     CDS             997..1458
                     /locus_tag="IVY70_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7959388.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1508..1972
                     /locus_tag="IVY70_002274"
     CDS             1508..1972
                     /locus_tag="IVY70_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7959389.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            1986..2735
                     /locus_tag="IVY70_002275"
     CDS             1986..2735
                     /locus_tag="IVY70_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7959390.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2735..3565
                     /locus_tag="IVY70_002276"
     CDS             2735..3565
                     /locus_tag="IVY70_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7959391.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3565..4212
                     /locus_tag="IVY70_002277"
     CDS             3565..4212
                     /locus_tag="IVY70_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7959392.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4310..5086)
                     /locus_tag="IVY70_002278"
     CDS             complement(4310..5086)
                     /locus_tag="IVY70_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7959393.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5230..6189
                     /locus_tag="IVY70_002279"
     CDS             5230..6189
                     /locus_tag="IVY70_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7959394.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            6306..6614
                     /locus_tag="IVY70_002280"
     CDS             6306..6614
                     /locus_tag="IVY70_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP7959395.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            6641..6940
                     /locus_tag="IVY70_002281"
     CDS             6641..6940
                     /locus_tag="IVY70_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAP7959396.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            7114..7665
                     /locus_tag="IVY70_002282"
     CDS             7114..7665
                     /locus_tag="IVY70_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAP7959397.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            7811..8104
                     /locus_tag="IVY70_002283"
     CDS             7811..8104
                     /locus_tag="IVY70_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959398.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            8097..8393
                     /locus_tag="IVY70_002284"
     CDS             8097..8393
                     /locus_tag="IVY70_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959399.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            8464..>9455
                     /locus_tag="IVY70_002285"
     CDS             8464..>9455
                     /locus_tag="IVY70_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP7959400.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNF"
BASE COUNT         3333 a         1366 c         1779 g         2977 t
ORIGIN      
        1 atatgacttt ataatactta ttagagtgag ggataaagaa atgagacaac tacacaaaga
       61 acagattgaa acagcaatgg cagcgttaaa ggaaaagaca attgatcatg tatattttac
      121 agcatgtggg ggttcacaag ccgttttaat ggctggtcaa tatctatttg ataaagaatt
      181 aactgtacct tgtcatgttt atactgctaa cgaatttaac tatgctatac caaaaggatt
      241 atctgaaaat tctttagtta tcacttgttc ccattctggc acaacaccag aaactgtaga
      301 agcaacaaaa aaagcaagag aatttggagc aatgactatt tgtttatcta atgaagaagg
      361 atctcctcta tgggaagcag ctgaatatcc tgttcactat gattggggta aagatgttga
      421 tgctagtgat ttaaataaag gaattcttta tggtttatta tttaatattt tacttgtttt
      481 agatggctct aaaaaatggg aaacttgctt gaatgaattt gataagttaa atgatcttgt
      541 tattgctgaa aagagcaaat ttgaaataac tgctaaagaa tggggtaaaa accaaaaacg
      601 agataaaatt atttacacaa ttggtagcgg tatcaattac ggagaaattt actccacagc
      661 agcttgctgg ttcatggaaa tgcaatggat taattctaat gctattcatt ctggggaatt
      721 tttccatggt ccttttgaaa tcactgatta tgatgtacca ttcatcttag tgaaaagtat
      781 tggagataca agatttttag atcaacgggt agaagacttc actaaaaaat tcagtaataa
      841 attaatggtt ttagaccaag ctgattttga cttagaatct atagatagtg tagcgaaaga
      901 atatattgca gctgtactta gtggcgttgt tattcgccaa atggtagaag cgattgcttt
      961 tgaaagaggc cattccttag atgttagacg ctatatgtgg caaatgaaat attaattaat
     1021 aggaggttgc aaaagatggc aaatcaatta tttcacattg tgattacaac acattctacc
     1081 ctttgtgagg gttatttaaa ggcaacaaaa ttaattttaa tgaatgacgc cacaggtatt
     1141 agtgctgttc catttaaaga aaatactagt ccagatgatt ttgaggagag tatgaaagaa
     1201 ataattagtc agcatcattt gcaacctcta attattttaa cggatttaat tggaggaaca
     1261 cctaataatg tttctattaa acagttgaac aacaatagaa ttcaaattat tgctgggatt
     1321 aatttacctc ttttactgga gttattaatg gcccagtcag ctggtaaatc tatagaagaa
     1381 atagatttta aggaattact gaataactct caaaaaagta tggttcatat taattcgctt
     1441 ttaaaagatg tcagttaagg tggcaatttc tattctataa attttaaaaa ttgaaggagg
     1501 aaatagaatg attaaattag tgcgtgtaga ccacagatta cttcatggac aagtaatatt
     1561 ttcttggact aaacagtatg atattaacca tattattgtg gctgataaca aagttccagg
     1621 agatccaatg tctgtgatgg ctttgacaat tgctaaacct gctgattgca aattagacat
     1681 tgttaaaatt agtgatgtta aagggattat ggaaacatcc ccaaatgata attacatgat
     1741 tttaattaaa ggacctaagg aagcttttga attagttcaa aatttaccag aaattaaaga
     1801 aattaatttt ggcggtgtgt ctaagaaaag cgagtccaaa caatatggaa aggcagtctt
     1861 tttaactcct gaagaattag aagccacaaa aaaaattctt gatcaagaag tgaaaatatt
     1921 tgtgcaacaa gtcccatctt caagtattga aaatgtagat tttagtaagt aaggggggaa
     1981 aataaatgtt attaaaagcc attttattag gtttagttgg tgtggtagct gttattgact
     2041 ccaggttaat tggtcgacaa aatattggta gaccactaat tttgagtaca cttgccggac
     2101 ttgtgctagg tgacttgcat acagggattg ttcttggggc atctttagaa ttaatttcta
     2161 tgggattcgt ctcaattggg gcggctggac ctcctaatat gcaacttggt agtattattg
     2221 ccactgcttt tgcaatttta actaatagta gtacagaaac agccttaaca atagctatcc
     2281 cagtggctgt agctggtgaa tttatgagta ttatcatgcg catgataatt gctcaatttg
     2341 gacatgctgc agataaagcc attgaaaata ataaatttag aaaagctcaa attattcata
     2401 tttattggtc atttattttt aatgcttttg tttactttat tccaatcttt ttaacagtat
     2461 attttggtgc tgacgttgtg gcagatttag tagataaaat tccagaaatg attacagacg
     2521 ccttgactgt ctctggaaat atgctttccg cattagggtt tgcaatgcta ttaagcacaa
     2581 tgttgagtaa aaaattatat ccttacttta tttttggatt ctttattgtt gcctattcag
     2641 gtctttcact aattggcgtg acaatttttg cagctctgat tgcctttgta atggatcaag
     2701 ttaaatatgg aaaaagagaa gaggcgcatg gttaatggaa aatagtacag aaaaaaaacg
     2761 tttaatcaca aaaaaagatc ttaataagat gtgtcttcgt tcatggacaa tcatgtgttc
     2821 gtggaattat gaaagacaaa tgcatatggg atttatgtat gggatggcgc caattttaaa
     2881 taaagtttat aaaaatgatg aagagaaaaa aagagaagct tatactcgtc atatggcatt
     2941 ttttaactgt acgcctcaaa tgacttcatt tattatggga ctttgtgctt ctatggaaga
     3001 acaaaatgct caagcagaag aaggaaactt taatactgaa tctatctcca tgattaaaac
     3061 cagtttaatg ggtccttttg ctggaattgg tgattcattt ttccaaggta ctttaagaat
     3121 tattacattt gggattggat tgtcttttgc taagcaaggt agtattttag gaccgatttt
     3181 agctgttggc ctttttgcta taccttctat tttagctgtt ttttacggcg gtaaattagg
     3241 atatgagtct ggcaataaat accttactaa attgtatcaa gaaggtctta tggatcgagt
     3301 aatgcaattt gcttctatca ttggtttggc tgtagtaggt ggaatggtgg ctagcatggt
     3361 gccaattgtc acccctttaa aattcactac agggggaaca gaacttgttt tacaagactt
     3421 acttgattcc attattcctg gattattacc acttgtatgt actttgggaa tatataaatt
     3481 gattaagaaa aatgttagta caaatatgct cttattaggt tgtattttgg cagggtttgt
     3541 tttaagtata ataggtatct tataatgatt aaggctctaa tttttgatat ggacggagtt
     3601 ttaattgata gcgagattat gtatcaaaag tgggtattta attttttaaa acaagaaaat
     3661 ttaagctatc ctttgtattc ttatcaaaaa aagattggaa ctacagcaag tatttttgat
     3721 gatatagaaa agtacaatga gggatgtaac agtttagaat tgaagggaaa atttactaaa
     3781 tattggaaat cgcaaaagat tgattacttg gatatttttc ctaatatact aattgaacag
     3841 cttgaatggt taaaaaagaa aaaaataaaa atggcaattg cttcttccag tccaatggat
     3901 actataaatg aagttgttac cacttgtaaa atagagtcat attttgattg tttaattagc
     3961 ggaagagatt tgccagaaag taaacctaat cctactatat tccttaaagc ttcagaacag
     4021 ctcagaattc cagttgaaga atgtctggtg atagaagatt cttataatgg tataaaagct
     4081 ggtaaaagag caaatatgaa ggttcttgca attaaagata aacgtttttc tcaagatgta
     4141 agtctagcag atgatgttat ttacgatatt aaatatctgc gacaagaagt acaggtgaaa
     4201 tttggaatat aaataagttt tttgatgtat ttagagaata ttagtaaaaa taaaaatcat
     4261 gcccaaatct aatcaaaatt gattgctttg ggtatgattt cttttaatat caatctatta
     4321 ctttaggatc tgaatcattt ttatgcaccg tatctaaagt gatattttct gtgacttcaa
     4381 taacataact aattctatca caacgaactg tggattgaga ataatgaaat ggttgattat
     4441 ttttatcttt gccaaccttt tctaaaataa atagcggatc tcctactata cattgcaata
     4501 agtcggaaag ctcaggtgtt gcagataaac cgtttatttc cataatagag ctcgtagctt
     4561 tgacatcata gtcatcttct agaagtttat ataatgaagt atattggcta cctttttcaa
     4621 ataaagttgg aaatttttct tctgatgcga aaaaattatc aattcccatc ggggcaccgt
     4681 cttcccacat taaacgttta atattgtaaa ctctatcagt tacattgatt tgtaatttat
     4741 tagctaaaaa ctcatcagcc tttataattt tagaatgtaa aacagtatat ctaattttat
     4801 gtcctcgggt agaaagagaa tcgctaaatc catgaactcc agctaatttt aaactttctt
     4861 ttttttgagt tacaaaggtc cctttccccc ttaatcgttc aactgttcca ctttctgcta
     4921 aaagtttaaa agcttttcga atggtaatct tacttactcc ataaatttta cataattctt
     4981 cttctgtagg taattgctct cctggaagat attttttttc acgaatatcg ttacgtatag
     5041 aagcttctac ttgtaaatat aatggtctat tatcttgaaa attcatagta cactccctaa
     5101 aaagttatac ttcattatat cacaatttat taaaacgtcg attgtaaaat taagctagac
     5161 aactaaaaag atcatttgtg ctacactcaa aatagttccg accaaagaat taataaggag
     5221 tgagtacaaa tgacttacac ccatcttaca acagacgaac tagtaatcat agagtcttat
     5281 ttcaatatcg agaaacctgt cgctcttgtc gcacaaagtt taaatcgctc tagacaaacc
     5341 atttataatg tttattcgtt tctcaaacaa ggaaaaaaag tgctagatta ttaccgtcaa
     5401 tacaaacaaa ataaatctcg ttgtggacga cgttcaaccg ttttatctga tgaacaatca
     5461 acatatgttc aagaaaaggt agctcaagga tggtcacccg atgtgattat cggccgtggc
     5521 gagtttccaa ttgattgttc tgttcgtaca ctctatcgga tgtttaaaaa aggccttttt
     5581 gacacacagt cgttacctat gaaggggaaa cgtaaaccga ataatcatca agaaaagcgt
     5641 ggaaaacagg cctttcgcag aacgattcat gaacggaaca gtctctatcc acacctgact
     5701 aaagaatttg gtcatctcga agtcgacacc attgtgggag ttaaacacaa gagcgctgtt
     5761 attacattgg ttgagtgttt atccaaggtt attattactt tgaaaccgac aggcagacga
     5821 gccgaagata tcgaaaacag gttaaatgat tggttcaaat ccatgcctaa acacttgttt
     5881 aaatccatga catttgattg tggaaaagaa ttttccaact ggcgatctat aagtaactcg
     5941 aacgatattg ccatcttttt cgcagatcca ggaacacctt cgcaaagagg gctaaatgag
     6001 cactcaaacg gcttgcttag acgagatggc ttacctaaaa caatggactt tagggacacg
     6061 gatgaaacct ttattcaatc cgttgcctcg aaaagaaacc atattccaag aaaatcatta
     6121 aattatcgaa caccattgga agtatttttg agctgcatag ataaagacat attgtctagc
     6181 ttaatttgac aaatcagata gtataaaatg aatcaaattg aatatcaaat atatattata
     6241 tccatgaagc ccaatcttct ttaatagaag attgggctat ttttatggca aaggagggaa
     6301 aagaaatgtt tcaaaataaa ggacaacgtt atgtcacaaa gggagtaatg gacagtttgc
     6361 cggtggaatt acaagcttta tgttggaatc taatcgatca aaacgttcaa aagcaatttc
     6421 atttagacta tcttcaaatc tttgaatttt ctacggaaaa aggaaatcaa aagttggttc
     6481 atcgtcaaga agaaccagaa gcaagaaaag agtatctgat ttcacctaag cttcgtttga
     6541 aatcagtgtc tcaaaaaata tgggtcattg attcgggtga gtatcaaacg atgttattac
     6601 cagaagaata ctgatatcta aaagaaagga acgaaaggac ttgttagaaa cggctatcga
     6661 agtactggaa aagtgtgcac agctagtcac tactcctgaa gagtggggct atgaatccgt
     6721 tacgatggaa aaagaggaaa ttgaaatggg tatgcttcct gaagatgtta ctctaccacg
     6781 tttagtgatg acacatcttt atatttattg cgcaccagaa gatggaaaag actatgtagt
     6841 ttatttcatt acagatatta ccagccagcg agaatttgtt cgtgggttac tggtagaagg
     6901 acgattggtt tggagtcaga ttggaggaac gaatgaataa gatcgattat ttgagaatga
     6961 ttgcgaaatg tttagaagat atccttacag cgattcatca aattctgaag aaaaaaggag
     7021 aaagaatatg aaatattttc aactagtatt gacacttata acggcaatgg tgattgaatt
     7081 gcttgaacaa aaattagcat aggagaaaaa gagatgattg aaaaagaatt agtgaccaca
     7141 caaacagaag tttggcgaga tcctcatcat acccatcgct atgttttcaa acgacaatgg
     7201 gcgatcaaag gaaaagaagc ggaagataaa ttagcggtag tcattactat tcgtccaaca
     7261 gatacagaac catttacgaa tgatttgagt atgttattaa tcgaaaagaa tatccgacaa
     7321 ttaggattta ctggttttat tgctgtgaat cttttttcgt atacaaaagc gaaaagtcct
     7381 cgggtatttc ctcgtggaaa tgatgaacaa tctttggaaa tattaacaac tgttttcaca
     7441 gaaaagaaaa tcaacaccat tatttttgct tgtggttcga ttacagcgac gagtcaagtg
     7501 gcttatgaac aagcgaagac catttacgat caattgagtg cgaaacaaaa aaagatgacc
     7561 aaagtattga taaatgcaga aggtaagtta tcccacccac taagtattca tgttcgtaat
     7621 gaatggcaat tggcagatag tcaattatta tttcaagaaa aataacattt tattttctta
     7681 tgttacttat aagtagtttt acatgctttt tgactttaaa aatacatgtg atgatacatg
     7741 ttatatgaat tatcaatgta cccagtaata tgctattttc attatgtaaa aaataacagg
     7801 gggtgtgtag atggagattg atgaaacttg tttgagtaac tcttataaaa gtatgaatga
     7861 cttttttgag gaaatggaac tggattcaga attgctctat caattttatc ttgcttttcg
     7921 agggcaacag atttcttttc caatgaaaat gtacgatcgt gagttagtta gaaaaagaat
     7981 cgaaaacatg atagaacagg aaaaaactgt agatatccgt caattgacag aagtttatgg
     8041 tttctctgca agatggattc aaaaagtaat taaacaaaaa gaaaggagac gagtacatgg
     8101 ctaagctgat tgtattaggt ggtttaattt acggtggata ttggttatat aaatatatgc
     8161 ttgcgcatcc gaatatagat aaaggaaaag taattaagac agttggggta atcatagttg
     8221 gactcatagc tctaactaag aaaaataata ctaatagccc aaaaataatg agaacttatc
     8281 gctgtagagg atgtggaagg gaaatttcta ctagttttaa tccatctgaa agccctcaag
     8341 gaagagatcg ttgtcatgct ggctatcgac attcttggga aaggattaac taaatgaaca
     8401 ccattgagat taattgactg ataaaataag tacgcgaggg agaaattgaa agggggaaat
     8461 aaaatgaaaa aatggttgtt attactaatt ggaatgggga ttttattcac tggttgtaca
     8521 tctgagaata cgaggactaa agagactgta gaaactgata caacaattga ggctaaaaaa
     8581 agtaaaacgc aatcaactga acaaactaca acagaaatga aacaatctgt ggaagcaagt
     8641 acaagtgaaa gttcaatcat tgaatcttct tctgaagagg ttaaaaatac tttgtgggat
     8701 agtgagaaat ctactcaact agcaattttt gtcccacaat ggggaaagac attaggtcaa
     8761 gaatataaaa gctacaatcc tcagaacaat gtaagtctgt atggtacgcc tttaccaagt
     8821 gcagttattg atggaaattg gaaaatggca atcaatgaag caccagttac cgtacaatgg
     8881 tcagaagatg gaactggtca tgcagatttc aatttagtag cagtttattc tgatgtagaa
     8941 actggagaat atttagggca gcatgtatat ttctttggct ttcaaaatgg acaacctaaa
     9001 gtttatttaa cgcaacaaaa tcaaggcaat gataaaaact acatgtactt caatgaaact
     9061 cagaaccaac agttaaaaca aggtttcatt gatattgtga atggacaaac tcctcaaact
     9121 ccagtagtag aagaatctac tcaacaagca acaactacaa cgacacaatc tacaagtagc
     9181 tcagcaattt caagttggga agctgctaaa gaaactgtac taaataataa agctttatgg
     9241 ttgtatgatt ctcctgataa aaatgcatca aatactaatt taattagcac aaatgaatat
     9301 atgaaagatc taatgcaaga tgaaaatgga agctactatt caattaatat tgtaacggat
     9361 gataatggaa atacaacaat ggctggtgga aaacaattcc gtgtttatac agatggaaga
     9421 atttctcaac gtattggaat gactaacaat tttca
//
LOCUS       DACCXO010000104         9397 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.107, whole genome shotgun sequence.
ACCESSION   DACCXO010000104 DACCXO010000000
VERSION     DACCXO010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.107"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            512..1315
                     /locus_tag="IVY70_002286"
     CDS             512..1315
                     /locus_tag="IVY70_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP7959401.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            1900..2355
                     /locus_tag="IVY70_002287"
     CDS             1900..2355
                     /locus_tag="IVY70_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959402.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            2474..3532
                     /locus_tag="IVY70_002288"
     CDS             2474..3532
                     /locus_tag="IVY70_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAP7959403.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            3574..5523
                     /locus_tag="IVY70_002289"
     CDS             3574..5523
                     /locus_tag="IVY70_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP7959404.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            5526..9167
                     /locus_tag="IVY70_002290"
     CDS             5526..9167
                     /locus_tag="IVY70_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959405.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
BASE COUNT         3389 a         1344 c         1932 g         2732 t
ORIGIN      
        1 cttttccttt ttttcaatta ttcaaagtta cacaagcaaa ataaactcct agatagtgtt
       61 agtttttttt aagatttttt atttttaaga ttttgaaata agagaggagt aatcctcctt
      121 tattaaatat ctgttaatat atctgttaaa tatctgttaa tatatctgtt aggggaggct
      181 taatctcttg ggagagtaag tattaaaggg gtataaaggt aacacttttg gacagtaaag
      241 gtaacaaaag tggacagtaa gggtaacagt tttggacagc aaaggtaaca aaagtggaca
      301 tttgaggtat cacactagga taagacgtta ccttcggttt tcttgatttg gaaggcgaaa
      361 aaatagaaaa ttagattagt aacaaatctt tgttgactgt tacctttagt tgtgatttaa
      421 tttaggtaac aagcagaaaa aattgtttcc tttaattagt accagattaa catgattaaa
      481 ggagcgcaaa aagaagattg gagagtaatc aatgtctcaa gaagttaccg aaaagattat
      541 tgatagtgat gtagatttag tatcagaaca aacaaatgca attgtgcgtt ataacaatgg
      601 tctgaataca acagctttgc gaggatttaa gcctgttgaa atggatgtgt tttgggctat
      661 tttatcaaaa atgaaacgtc aaggaacagc agaagtaact tttgatttca acctctttcg
      721 ttatttggtc aaatacgaca gaagagaaaa ggacagtttt tattctgtgt taaaagattt
      781 gagtgacaaa ctaggtaatt taacctaccg ttttgaagat tcagacgtct atgaacagct
      841 gtggcttttt caaaaattcc ggatagataa acagaaggaa acagttacaa ttaaggcaaa
      901 tgaggaattt gaatttatct taaactcaat tggcaaaaac tttactcgtt ttgaacttga
      961 taattttaca aaactaagaa gtagctacac aaaagaattg tatcgtcatc tcatgcaata
     1021 cagaaataaa gagtggcgaa atggttattg ggtagtcaaa gttgaagatt ttagaagagc
     1081 attaagtata ccttcaagtt atagaatgac aaatattgat aaaagaatac tagagcaggc
     1141 gaaagaagaa tttttagctc ctgatgaaaa cggaatacag gtatttgaaa cttttgatta
     1201 tgaaaaaatc tatgcaaaga aaggaagaag agttgatcgg ctcagattca cttttagtga
     1261 gccagaatca aacttgccaa ccatttcaat gcataattgg ttagaagaag attgagaaga
     1321 agcaggttgt aattttaata caacctgctt ctttaaaaaa gtatgggtaa cacttttgga
     1381 caataaatgc aagaagtttt tttgtaagca agtttcagtc taaataatat ttgaaagaat
     1441 ggctaatcta tacaaatttt aacaactatg ggtaacactt ttggacaagt caagaaaaaa
     1501 aacaattttt cttaatttgt tgatcgacta aaggtaacac ttttggacaa cacagaataa
     1561 aaagtccggt gatatcagtt ttggtaggaa acatcttaat atctttgtga gattgaaggt
     1621 aacatttttg gacaagtcaa ctatttgtta aaagttaata tagagtaatt agataagttt
     1681 tttcttttcg tctgataaat ggataatatt agttatttta ttgctaatta gtctttcatg
     1741 atgactgaaa tagctaaaat aaatatctcg ttggataatt gctgatactt taagggatat
     1801 ctaaatttgc tgattgattt atttatgtta ttatgatatt ataataacat aaatgtgtta
     1861 cgcccgaacc tcattttaaa aaaatgaagg ggtgagacaa tgaatgctat taattggcat
     1921 gaatttgtta actatatgga gagcttacta gatgatctaa aaagttttag tattgctgat
     1981 gcagtaaagc atgggttacc agttagtaca acgatgaaat tacgaggatt gaaaattcca
     2041 gatgaaatta ttaagctgag aactaaaaca attattgaaa ccgcaaaagt cgttgacaat
     2101 atgaaccatg aactaaaagg caagaaaaca acttacgaga aagattttga acgtttttta
     2161 aaatctgtta ttggaaaata tctaggtaaa aaatctttca gtgaattgag agatgaagag
     2221 attgtaccta ctccaatggt tagcaaaatc cgaaaaaaca aagccgaggg gaattactat
     2281 cttggtacaa tagcaaaaat cattgattat ttggaaaata aagagcaaaa gaaaggaacg
     2341 aagaaaggaa gataattttt gtgttaaata ttaatgggga agaatatacc gaaatgattc
     2401 aaaatcccga acctagaatt accgaaaaaa cgtctgattg tttgctcttt tatgaaaggg
     2461 ggcagacaaa ctaatggaaa aaaacgtcaa ttcaacatct accaaagatc aatacagtat
     2521 tattggaggg ttgcttggtg ataaggataa gagaacacgt cacggattac gcattatgct
     2581 tgcaactttt ctaggagtgc tttttttatt aacaggcttg ggtagctaca tttatcaatt
     2641 aggaaactat aattattatc tcgtgaacaa tacaacacct ataggctctt cattggagtt
     2701 tgctcgatca ggagcagaga tcactatttc agatgtgtgg acagatcaaa acagagatgt
     2761 aacagtagta aaactcggtt actcgcaaaa cgctagaaag ttgttatcta cgcaaggaaa
     2821 aaactacaac ttatatatgg caacgaaaga aaaagatcgt cctaaaaagc ttgaaatgag
     2881 ctatgggatg ttaagtactg atggggatgc tttcttattc ctaaaaggta aattagatga
     2941 aagagcttat caagttttta ttgcaaacca agtatcacta atttctggtg atgatacagc
     3001 tgacgatgat accaaagtca atgtgaatga agcagattca gttacaaaag cactttctca
     3061 atattctatg aatgatgtta ataatgaagg agtaatttct tttgggaaaa aagcaaaacc
     3121 tgttaatact cctgataata tcaatttcag aataaatcca tattcagatt caactaatgt
     3181 ctatcaagga agctttctta ccaaagatgg cgatattgat tattcaaaag ttgtagcagt
     3241 tacaagtaag aataatgttg taagtaagtt agaagatcag ttaaatgctt caaatactaa
     3301 catcaagaaa attgaagcta caaaagctga gtatctggaa cgtttacaac aaaatcctga
     3361 cgataaagat tcgcaagaaa gtattcgtca actagacagt cgaatggaga ccgaaattaa
     3421 agcaatcaat gatacacaaa aattgattga tcaatacaaa aatgctgact ttaacaaaga
     3481 ttcttttgga gacatgaaga cgacatttaa gtacctaact ctgactgact agaagtaaat
     3541 tattcttatt ttagaggaag tgaacacaaa cagatggaaa atagtaccga gttttttaag
     3601 aatattcaaa ctgaatttaa tcatgctctc acaccaatga aaccaccgaa acataagtta
     3661 gtgggacgtg atgacgaact ccatgagttg tcactgatct tgaatagacc ggaaacaccg
     3721 caagctatct tgttaggtga agccggaaca ggtaaaacag cacttgcaag tgaatgggct
     3781 gaaagacagc gacaagatgg aaagaaagta caagtataca aattatcaat cggacaattg
     3841 cgtgttgggg gagatgatgt cctccaaaca cgtttagaaa cattaattcc aagattgaaa
     3901 gaatatgagc gaattttaaa atctgaggac aaagaaaatg aatttgtcct cttcattgac
     3961 gagtttcata aagttgttag ctcttttggt agtggcaata aaattggggg tgattcgcta
     4021 aaagaaccgt tagcaaatga atcacttcaa ttgattaccg ctactacaag aaaagaatat
     4081 gaaaactata tcaaaagaga tgatgcctta gggagacggt tacaaccgtt agaattgaat
     4141 gaactaaaaa gcgatgttgt cgtcgatatt ttgagacaat ggctaaatac atcctctgtt
     4201 aaagatgatc ctcaggcttc ttttaaatta tcgcaaagga ttcctgatga attacttcaa
     4261 gaaatcgtac aagcaaacaa gctttatcgt gaggatttgt ccgaaccggc taaatcaatt
     4321 gccgtaatgg aggatttgat tgcaaaagat cgagtagacg gaaatgctta taatcgagaa
     4381 agtctattgc aaatatttgg taagtataat attcagctag attctgattt tgatatggaa
     4441 aaacttcaag aggaattaaa gtccataaaa gggcaaccgt tagcagttta tactataaca
     4501 atgttgttta aacgtatgaa gcttggattt gatcgtttta aaggtcgaaa gcaaccaaaa
     4561 gcaactgctc tgtttacagg atcaacaggg gtcggtaaaa ctgcaatggc taaagccgtg
     4621 gcaagaggac tttatggcga tgaaaataaa gttctcttag ttaatatgcc ggactacaaa
     4681 acaaaagatg atgcaatgcg ttttagacga ctcataggta ctcatgtaag gcatgatcct
     4741 tctgcgatta ttgtgtgtga tgaatatgag aaagctggtg tagaaacact cgatgccttt
     4801 ttatcaattt tagatgaagg taggataagt tttactgaaa gaggatggga tgatcgagaa
     4861 gttcacgaca ctgtttcatt gtcaaataca attttatttg cgacaacaaa tgcaggtgcg
     4921 gagatgttcg attcgttaaa cagatatagc tcactatctc gtgttacaaa caaggaaaat
     4981 tctaatgaat atagcactga actaaaaaga gaataccgaa aagtcgcatc agggatcaaa
     5041 caagccttga ttggtgaagg tctaaagcct gaagttatta accgatttgg acgaatagtt
     5101 ccattttggg ctttatccga acaaactctt ctggaaattg cttataatga aatgttgaag
     5161 agtgctgaat tagtacgaaa acatttaaat atagatttac gtcttccacc taggaaagaa
     5221 agagtatatg gtcaaaattt ttatgcagac gatctctcca tgcacttagt ttttgacatg
     5281 gcttcacaag aggattctaa atcagggggt gcaaggaata tcttgcattt agttgaatct
     5341 gagtttgaag aagcgattac tgaagcaata attacttatc caaacgcaaa aatattcgat
     5401 gttaagttga gtgaagccgg agcgtttgaa ggaaaaggtg attctaatgc tgaaattatt
     5461 gcaaagcctt ccgttgctga aagtaaagaa aacaagattg tattttcgat tggaggggcg
     5521 tagctatgaa attcaagaaa tatttactac aaaaccttag aataaaacga tgggtcgtaa
     5581 gaggtctagt tattggagga gtattagcag ttactttagg cgctcctttt ggaatgaatg
     5641 atccaactcc tgacaatcaa gatagcaaac aagaaacagt tataaataag aacgttgcat
     5701 atgcggaaag taaaccggca gaacaaggaa tgtggacact atatgctcgt atcctaatgg
     5761 gagataaaga agttaatgat agtgccgcta aaactggaca aatgaatttg ttaggacaag
     5821 gtggtatcag tgccgatttc ccttattctg acatttctaa acaaggtaat tcgattgcag
     5881 ggggtaatgc aggtactcaa ctagcatcta ctttagcaac ttatagttac tacggatata
     5941 ttgaaacagt ctcaggaaat gctattgcca gtcaagcatc atccattcta ggaagtattg
     6001 gtaggacgat tggtagtttt atcggatcaa ttagtttaat cctttctaat attgctgaat
     6061 ccataaatga actccttgga aaatttttca tgagtatgaa tatattttct cttatggggt
     6121 ggggagaagg tggatcggat gtatctaatc cggtatctaa agcattaact agcttgctta
     6181 acaatttggg attaaataat gaattattta aagcgatagc aagcttatct atgctctttt
     6241 tcacagcaat actaggttat caaggaatga aattattgtc gaaagatggt gttaactcga
     6301 agggattatc accagtactt gaaaaatgga ttgttcgtat ttttgtattg tttatattct
     6361 taccagtggc aggatcgcta ttgggagttt atatcaacgg catccagtca gcaaaaaaag
     6421 atggagatat accaacaaat gttgtttctg actacatcat taacacaaga ctttgggctt
     6481 ctactcaaaa tcttgctcca tcatctgata ctggtggtga tgtacctgac ggaaagccta
     6541 catccgaaat caagttcgtt gatacagatt atgaaccatc taaaaaatct gcaatgataa
     6601 gagcgatcaa tactagcagt tatgccagac aaaataatga tctagattct aattcagtcg
     6661 gtatggctct attaaaagaa tggtcatcga atgatacttt caatattaat acttatgccg
     6721 gtgatgttaa tgtcgccgaa aatgatgctt atatgggaag agcgcctgct gtttggacag
     6781 gctataattt aggggatgga aaagaaggaa gtgcaattaa gaaaccaacc aatttaaatg
     6841 gatatatttg gtcagcaaat caaaatgtga cagacgacaa tcgcaagcct gatggaaaag
     6901 gaacagcgca taactttgat gctactcaac cttggggcgt tgctaacaat gcaactttta
     6961 gcacacagtc ggtagcttta ttgctccaat cggcacttga tacatcggga gctaagtttt
     7021 atgcgtataa tatcgctcct tcaggaattc aaggcagtgc taagaatatg tcaacagtaa
     7081 aaacagaatg gcgtacagca tccttagttg gtcatggagc tatgggtaag tttggctcgt
     7141 ggctagcgat gaacgctgaa tcaatcgttt tagcagttct ttacttagca tgtgcttggg
     7201 cgatgttcaa tgtgaatatc gtgaaagcat ttaaacgtga atttattcgg atttgggaag
     7261 tagttctaac aggatcactt gcatcaggag caggtgtgat ggcaatttca ttaggtgttg
     7321 ttacaacatc tctgatcgga ctacaattac ctatctttct gattactttt attcgtgcat
     7381 ttggtgatac tgctgtggga ttcattccta atacattagt tggcgatggc gtagcaaata
     7441 ttgttcgatc tgctatgatt ttaggtgtag gatatctttt agtctttaaa aaaggcgaaa
     7501 aagtaaataa tttgattaat atgcttattg gcttaccact tgaagtagcg tttgggtttg
     7561 atgaacgtgc taagttatta tttggtaata gaaatgatgc tgtgcgagtt gtggacggcg
     7621 tttctaaagc aatcaagaaa agccaaggag ctaaaaatcc ctattatgat ggtggcaaca
     7681 gctcagtaaa agatatggtt gcaggaggtc ttggagcagt tggagcgcaa aaaatggctg
     7741 aacgtttgaa caatatggga gatagcaatg ctgatgctaa gtccattgct gataaagtaa
     7801 ttcctgaaac accagaagga atgtcattca acggtggcgg agttggttcc acaatcaata
     7861 atacctcttt caaagattta gttcgtcaag atcaactacg taataactcc caacaatata
     7921 ttgatcattt aggacgtatg gttgatgaaa acggaaactt tgtcgatgaa aatggtgaaa
     7981 tattaaaaga tagtaaatac ggtaaagatg ctaaaggcaa ctttattgac gaaaaagggc
     8041 gtttggtcga tgaagatgga aaccttatag atactaatgg taaaccattg gaagatagtg
     8101 aagatggtaa tattggtatg gatagcgaag gacgtttaat tgatgaaaaa ggtcgcttag
     8161 tcgatgaaga tggaaatctt gttgacgaaa atggacgcag gttgctaaat ggtgattttg
     8221 gtcatgatag taacggacgt ttagttgacg aaaaaggtcg tttagttgat gaagatggaa
     8281 atcttgttga tgagtttggt aataaacttg gtaaacacag cgatggatat aatgtgaatg
     8341 gagatttagt cgatgaggat gggaatatca ttgagacgag agatgcgctt gtggcagatc
     8401 gaaatagaga actcgaacaa caacggcata cgccaattgc tgttgcaatt cctgagaatg
     8461 aagaagacga gattacttta gatcaaactc atcaagaact gaggaatgaa atgagtggtg
     8521 aggacgtgtc agacagagtg catcgagaat taggagatgg agcatctggt gaaagtcagt
     8581 caagagagct taacagcaac tttgataata gtgctattcg gaatgacaca gtacttaaag
     8641 acagagaatc gctaacaaaa gaaacggata aagaacaatc agatgtgaag aaaaagttca
     8701 ttcctactcc taatgttaat caaacagata aatatgaaac aaatattgga tcaggaatgt
     8761 cgaaagacaa tcaatcaatt agagaacctg aactgacaaa taaaaataat agaacaaata
     8821 attctaaatc tacttcagat aattcaggga aatctaatca atcaaccaag aaaacatcaa
     8881 cagctaaatc agccgttgaa tatctatcaa aaactggtaa aaatgtttac acctcagcag
     8941 ataaagcttt agcaaaagct ccaaaaggta gtctagcagg gataacctat gaagctaaaa
     9001 acgaagctat taaaataggt aaaggaacgg ctaaggttgc caaggctact gctacaggag
     9061 catccaaaac tggtaaagta gtgaagaaat ctaccatgtc agtcaaggac tatattgtaa
     9121 aatacggtaa tcaaaaatca actgaaaata agtctgaaga cgagtaaaaa gaaggagcat
     9181 tgatttgctc cttcttttta ctttgatttc taactgaaga tgtcaaggaa aatttctatt
     9241 tatgcaaaaa aattatttta aattgtctat aaatgaatta aatatttact gttcaaagtt
     9301 gtataattaa aaagggctta tgctggtaaa gaacagaatt attatctaaa caaaaacttg
     9361 ataaaccagc atttttagcc aataaaaaaa gtgaaat
//
LOCUS       DACCXO010000105         9373 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.108, whole genome shotgun sequence.
ACCESSION   DACCXO010000105 DACCXO010000000
VERSION     DACCXO010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.108"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(15..446)
                     /locus_tag="IVY70_002291"
     CDS             complement(15..446)
                     /locus_tag="IVY70_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP7959406.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(464..784)
                     /locus_tag="IVY70_002292"
     CDS             complement(464..784)
                     /locus_tag="IVY70_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7959407.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            1083..1859
                     /locus_tag="IVY70_002293"
     CDS             1083..1859
                     /locus_tag="IVY70_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP7959408.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            1874..2077
                     /locus_tag="IVY70_002294"
     CDS             1874..2077
                     /locus_tag="IVY70_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7959409.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            2093..2431
                     /locus_tag="IVY70_002295"
     CDS             2093..2431
                     /locus_tag="IVY70_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP7959410.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            2418..2597
                     /locus_tag="IVY70_002296"
     CDS             2418..2597
                     /locus_tag="IVY70_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959411.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(2640..3110)
                     /locus_tag="IVY70_002297"
     CDS             complement(2640..3110)
                     /locus_tag="IVY70_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959412.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            3197..3895
                     /locus_tag="IVY70_002298"
     CDS             3197..3895
                     /locus_tag="IVY70_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP7959413.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            4073..4414
                     /locus_tag="IVY70_002299"
     CDS             4073..4414
                     /locus_tag="IVY70_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959414.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            4407..5078
                     /locus_tag="IVY70_002300"
     CDS             4407..5078
                     /locus_tag="IVY70_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP7959415.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            5084..5770
                     /locus_tag="IVY70_002301"
     CDS             5084..5770
                     /locus_tag="IVY70_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959416.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            5773..6522
                     /locus_tag="IVY70_002302"
     CDS             5773..6522
                     /locus_tag="IVY70_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7959417.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            6534..6803
                     /locus_tag="IVY70_002303"
     CDS             6534..6803
                     /locus_tag="IVY70_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959418.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            6965..7267
                     /locus_tag="IVY70_002304"
     CDS             6965..7267
                     /locus_tag="IVY70_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959419.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            7264..7425
                     /locus_tag="IVY70_002305"
     CDS             7264..7425
                     /locus_tag="IVY70_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP7959420.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            7422..7727
                     /locus_tag="IVY70_002306"
     CDS             7422..7727
                     /locus_tag="IVY70_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959421.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            7727..8083
                     /locus_tag="IVY70_002307"
     CDS             7727..8083
                     /locus_tag="IVY70_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP7959422.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            8043..8288
                     /locus_tag="IVY70_002308"
     CDS             8043..8288
                     /locus_tag="IVY70_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959423.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            8285..8704
                     /locus_tag="IVY70_002309"
     CDS             8285..8704
                     /locus_tag="IVY70_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP7959424.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            8701..9258
                     /locus_tag="IVY70_002310"
     CDS             8701..9258
                     /locus_tag="IVY70_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP7959425.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
BASE COUNT         3496 a         1441 c         1918 g         2518 t
ORIGIN      
        1 aattcttgat gtattcatac atattgcctt gcgtaagaat attttttctt aaaatagcat
       61 tggcagacag cataacaagt gtatctgagc ttattctgaa tagaataata ctccataaat
      121 ttttcgagat taaactgaga ttcatcagtc agttcgttct caatatagat atttaataaa
      181 attagaatag ctattttatc tgcttctgtt tcaaattttg agtgaaaagt tgtagaagta
      241 tcgtacaaaa ctgaaaattc aaaatgagag gcgctgaaat gtgcaagttc atgagataaa
      301 tgaaaggctt ctgcagtttc accgtataga ttttcattca aaaaaatgat tctgggtttt
      361 ggatagtaga aaccaggctc tttcatctcc atataaacta ctttcaaatt gtattcgctc
      421 aacatttctt tcaatttcaa atacatacaa accatcactc caactattca ttttcctcta
      481 aagctttagc aattgcaatc gctttacgca ttgtctcctt agatatttct tttccatcaa
      541 aagaaaaaac agtatcgtct tctgataaat ccacatgttt aggggtttct ctttcttctc
      601 tacctagaag gtagtctaca gagacatcga aatagtcagc aatccgactt aattcatctg
      661 aattaggagt attatttttc cactttgcta gataaccgtt agaatatcca aaattaattt
      721 ctaactgccg tatagatatc tttttcctct tcgccaagtc ttttattatt tcataggtat
      781 tcatcgataa aacaaccttt ctaaatgttt acaaaaaaag tttagaaaaa tacgcagaaa
      841 taatttgact aattctgagt aatgagctat actatatctt gtaaacagat ttaacaacta
      901 aaaagacaac aaaaaacact attgatatgt aaatgcagac cgccaagaaa gctttaaaat
      961 caatgttttt atgtcttatt taattatgct cttagtatag agtattactc agatactgtc
     1021 aattgaattt agaaaaaagt tgttaaattt gtttacgaat ataaaagaaa ggaatgagaa
     1081 atatgaacac accgcaaatt tttaatttcg aacaaaacga agttcggaca attttagtaa
     1141 atgatgaacc atattttgta ggcaaagacg ttgcgagtgt tttgggttat tcaaacacta
     1201 aagatgcttt gtcgcggcat gtagatttgg aagataagat ggggtcgcga atcacgacct
     1261 caggtcaatc aagagagatg acaatcatca acgaatctgg tttgtacagt ttaatcttaa
     1321 aatcaaaact tccctctgcc aaaaaattta aacgttgggt aacaagtgaa gtgttgccag
     1381 caattagaaa acatggaggt tatctaactc cagaaaaagt agaagaagct ttgcttaatc
     1441 cagatacaat cattcaatta gcaactcaac taaaagaaga aagaactgga agattaatcg
     1501 cagaacaaaa gattgcagag tacgaaccta aaatctccta tttagatagc atattatctt
     1561 ctacagattc agtaacaatt agtcagattg cagcagatta tgggatgtct ccacaacaga
     1621 tgaataaatt acttcataaa ctaggtattc agaaaaaagt cggtaaccaa tggttattgt
     1681 gcaaaaaaca catgaaccaa ggatacacaa aatctcatac aactgagatc ccgaaagccg
     1741 atggtggcac taaaattgta atgaatacca aatggacaca gaaagggcgt ctatttatct
     1801 acgaattact aaaaaaagaa ggatattacc ctcaaatgga tttagaggaa attggttaga
     1861 aaggagtttt agtatgactg acattgcaga aatcactcaa cgagatagag aaaaaatcaa
     1921 agaatatgtc gaaagttcaa agttcttaac ttacaccatg cttgctgaaa gatttggaat
     1981 tagcaaaagc tacttatctt taattttaaa cggtaaaaag acttctgcag aagcaaacag
     2041 aattatagat tcgattatca ctatgtacga attgtaaagg gaggaacagc taatgcaata
     2101 tctagaagca aaaatcccaa ttccagaagg ctatgtaatt atctcccaag tggattatga
     2161 ggagttaaaa aaagctgatg atactggtag atggatgacg ttgccagaag tgctagaacg
     2221 gattaacaga aaatatgatt ggtttacttc tagagtttta aagaacccaa gatatagaaa
     2281 tattatcgat atcgagaaaa acaaaaatgg atttgtctat tatccagttg aaggaagaga
     2341 cacatatcta tttttaagaa gtaaaacact tgaattttta gaaacaaatt tttcggaaat
     2401 cttaaggagg caagcggatg ggaaaattta atagagcgct agtgttcagc gcaccgctaa
     2461 tcatctacgc tttaggactt tggggaagca ggcaagcgtt gataggaacg attgtttaca
     2521 tggtttggat ttttatgggg cttgatgaag ctgaagctga gtacagagcg aaaaagccaa
     2581 ccgagggggc tgactaaaaa tgaatagaaa agagaaacta gaatgtatat tactattact
     2641 cagtttaatt ctttcactaa tttctctatt gggaagtttg tatttttgat atcaacaatt
     2701 ttgtggattt gggttctatt tagagtaaat atcatttttg tttggtgatt aaggagttta
     2761 ttaaaattaa ttttccctac ttcaaatgca aggtaacaag atatagcact tttaggagga
     2821 atatttagcg gtacagcttc agagtatatt ctattatcat cgactgatcc tcctttagca
     2881 attaatttct taaatggagt agctgaccat ctgcggttta agttagcgga tgaaagttct
     2941 aaattaacta acatagctgg ttctgttgaa tagttagaaa tgattacttt tgtataaatc
     3001 atatctgcag cgaagtaaga agcattaagt tcaacttcta gttgagggcg cattcctttt
     3061 atttttatcc ctgttaaaaa cgtactcaat ataaaactta ttattgacat ccattgaaaa
     3121 attgttaagt gaaaaaaatt caaaataaac accaccagtt tttaactaaa ttataccaaa
     3181 aaggagagaa gaaataatgc aagaattagt aattttgaaa aataaagaag ctgtgactac
     3241 gagcttacaa gtggcagaaa gcttcgagaa aaaacatcaa catgttttaa gagatattga
     3301 tgcactaaaa aaagatgtgt ccaattttgg acagatgttt gtagaaggta atgaaccaga
     3361 ttcatatggc agaaatcgac gagttttctt cattagtaga gatggatttt tcttgctggc
     3421 tatggggttt acaggaaaga aagctatcta cttcaaacaa aaatacattg aagcattcaa
     3481 cgaaatggaa gatgttattc gcaagaatac tgttcctcaa acaattgaag atatgatgat
     3541 ctatcaatta gaagaaatga aagatgttaa aaaagatgtt tccatgctta aagatactat
     3601 gcgaattagc ggacaacaag agtttgaaat taagcaaaaa ggaaatatga aagttatggg
     3661 agttctaggg ggaaaagaaa gccgagctta tgaagaaatc agcaaaaaag tattctcaaa
     3721 attttggtct gaatttaaac gtaccttttc aatcccaaga tatggcgagt tacctcgtaa
     3781 gagattcgat gatgctgttt catttattga aatgtggttg ccagaaactg cgatccgtat
     3841 ggaaattgat caactgaaca gacaacagag actttttggt gatgacaatg aatagagctg
     3901 aagcgctaag aatagggacg gcaattgcta attgctggtg gaaatactat aaaccaatca
     3961 tcctaagcca acaacatatt gacaagcaaa aagcatggca acaaataaaa aagtgactcc
     4021 gccggcaagc aaagagtcac aaagaaaaca catcataagg agattttagc atatggaaaa
     4081 agaactttcc actctagatc aatatttgat tgatcctgat tggggcaagc cgaaaattga
     4141 ggaaacaagt ggtcgaaaaa tcagacgaaa tcttttgacg aatgaagaac tagcttggga
     4201 tcaagatgat ttaggcaacc atgtaactat ttgggatcat gtttatctta tccatctatc
     4261 gaagcattcg aataaacctg aatatattta cgtcatcgaa gatggcttga ttgatgcgct
     4321 agaagagtac gacagagata acttgattga tatctcttat tacggaccag gtaagaaata
     4381 cattgctgaa atggaggcag aatttgatga gtgaaatcaa agggacaacg aactttgaaa
     4441 aactttttag tcgtaagtta aataaaattc tcaagaaaaa aggaaatttt gattatttat
     4501 cttgggctca cgcgtgggag attatgaaaa agaatgatcc acaggcaacg gtaactatta
     4561 atgagtacaa acactacagg gttgtttctg gaactcatca agactttctt gttgaggaat
     4621 ataaaccttt tcttatggat gaaactggga cttatgtatc tgtctcagta acggttaaag
     4681 gacacacgga aaccgaatta tttcctgttt tagattatcg aaaccaacca gttgttaaac
     4741 caaatgcaat gcaaatcaat aactcattga agcgatgctt tgtgaaagca ttggctctac
     4801 acggactggg attatatgta tttcaagggg aagatattcc aacaccacct agaatcgata
     4861 caaagaaatt aaacatgcta gagacgattc tagaagcttt caatgagcag atgggtaaag
     4921 atatgaccaa aaccttaatc gaatatgtta atgagcagac agataaatta gggctcttag
     4981 ctgataacgt tgaaactatt gaacagttaa gctatgagca atgtgccttg atggagcgag
     5041 caatagcagc taagagaaaa gaattagata agaagtgata tgagtgttta aaccattaat
     5101 cgattcatat tcagcggttc tgaaaaagtt caaaggaaaa gacataagcg caaccatcaa
     5161 tgaggaagtg aacattgatc gactaaagac gatgtatgac ggctacgatg gtgatcgagt
     5221 cattgaaatt cgttttattg atcctagacg tttcaccgta cagcaacgaa acttcatcta
     5281 tgcgctgata ggcgatattt ttatcgatac aggcatgcca acggacttct ggaaggaatt
     5341 cttctacttt cgttttgaag gtgtcacagg gcgcaaaata agcctgaaag acgaatcgaa
     5401 tacaactgtg agtgatgcca atgtcttagc aaatatcatc ttagatttca tctttgaaca
     5461 tcatattcct ttcaaagaag gctatgagat tttacctgcg aatcaagaat attacttcta
     5521 caaatgcatc acaaaaagag tctgctgcat ctgtggcaaa acaggagctg acatcgatca
     5581 ctttgacaaa gcgctaggaa gacgaaagcg caaagaagtt gatcatgcag agtacacatt
     5641 tgcagcactc tgcagaatcc atcacacaga gaagcacaaa ataggtgtga ttaatttcaa
     5701 aaataaatat caaatcaaag ggatcaaatt aaaccagaaa acaatcaaaa agttaaatat
     5761 tggagggtaa aaatgacaga acatcgaagt tattatgcga ttataccagc caacgtaagg
     5821 tacgacaaaa gacttaaacc aaatactaag ttgttatacg gagagataac ggccttgtgt
     5881 aatgaaagag gcttttgttg ggcaggcaat gagtactttg cagatttata tggtgtgaat
     5941 aaagagacca tatcgcgatg ggtaagtgat ttgattaagt ttggatactt gaatcgggaa
     6001 atcatttaca aagagggtac caatcaaata atcaataggt acctacgaat taatcaatac
     6061 cctattgacg aaaaacgcaa tacccctatt gacgaaaaag tcaaagataa taatacatct
     6121 attaataata catttaataa tacaaaagaa tatataagag agttaccgcc ttcgaaaaaa
     6181 tcgaaggcta agcccgtccg tcataaatac ggagagtata aaaatgttct tttgtcagat
     6241 gagcagatgg agaaactcaa aacagaattc cctaatgatt accaagagcg aatcgaacga
     6301 ctgtcagagt attgtgaatc atctggtaag acttataaaa actatttggc aactattcga
     6361 agttgggcaa ggaaagaaaa aagtgaacct aagaacgcaa gcagtggata caagcgcaca
     6421 ggaagacgag agaagcttcc tgaatgggca atcgaccaag aagcctatct caagaaaaaa
     6481 gcgctagaac gagctaatag acaatcaaaa gcaccattct aagaggtgga aaaatgaaga
     6541 tcgattatct agaactaatt aatgaaatag cgaattataa aaagggcgag gaattagacg
     6601 tcctgagaga cgtatatgat caactcgaag aagctggaat tgaaggaatt aagaatgatc
     6661 gttcgagttg gagtaagctc agatactatt tcgcacttta tatcgataca acacaattaa
     6721 gaaatttagc atataccaaa ttactatttg ttgattgtgt taaaggcttg caaaaacatc
     6781 ttaatgaact tgagcaggtg taatcagatg gacctaaaga catttacagc acagattgaa
     6841 ctaatgcatc aagaagcttt aagacaaagt gtatcgtacg aagacaagtg gctcaacacg
     6901 ttccatggcg gacgtgagag cgcacttgat caagtactca aattactgaa aggagaatgt
     6961 cgggatggat aagaaagcgg caatgcagcg aattatcgaa ttgacttatt cagaagattg
     7021 gcaaaatgac aaagaagctg cttcagaagt gatgaggctt ggaagagcga tgtgggcaga
     7081 caagagcaac aagccaagac cacgaaaaat cgcaatttgg cacggtgaca aacttctagt
     7141 gatagggaca gctgaacagt tagcaagtct cacaggcttg cacgagaaaa tcgtgaggaa
     7201 aagagcaagg tgtggctaca cagacgctaa gaagagaacg tttagatacg tggaggaatc
     7261 atcatgacaa cagaagaagt gattcaaatg cgtattcgaa gccttcagcg tgagattgac
     7321 gatctggaac gaacaaaggc agtgatggtc aatgaaacgg cgagaaaggc aatcgatttg
     7381 cacatagaga atttaagaag ggaaatccat cgattggagg aatgagcgtg gataagaaag
     7441 cagcaatgaa aagaattgct gaattaacca agtcagaatc ttggcaagaa gacaaagaaa
     7501 tagttgcaga agtccaaaag ctcggtaaat caatgtggac tgaaaagccc aaacggagaa
     7561 cgccgagaaa aattgcaatc tggcatgatg accgaatttt agtaacaggt actgctgaac
     7621 agttatctga aattactgga ttaagcaaaa acattatctg ggatagagct aggagcttat
     7681 ggattgattc aaaaggacga cagtttaggt atgtggagga gagataatgg atctcattac
     7741 acaatacagt gacatcatcc tcaagaaaat catgatgaag attcagaaag ataaaaaatc
     7801 aaaagaacga gctgaattag ttaagttaga aatggctgaa acaggagcag gagtgcgaag
     7861 tagcaggcat tggaaagcag cagcaaacat tgaattttat tacaacgaaa ttcaaaaagg
     7921 gttcgatcag atgcgtgagc tggatcggca aacaaattgg agcaagaaac ttcatcaaga
     7981 tcgtttcaaa tttgtagaga agtataaaga aatattagaa gagtatttga ggaggacagc
     8041 aaatgataaa aaaactcgtt caattcagca tggatttata tgatatcgaa tcaggagcaa
     8101 cactatctgt ggaatcggac catctaatca taaattttgg tggaaagcgc cagattattt
     8161 tgtgggtagt tgatgatgta ctgtttccag aaattgttca tgatttcgaa gaatcaaaag
     8221 cggttgagtt tgaaatagtg aaaaaagtaa tggaattgat tgaaaaatac gaggaggaca
     8281 gcgaatgata ccgaagttta gagcgtggga taaacgaaag aacgtaatga gagatgtagc
     8341 cgtcttgcat tttactaaaa acggcaaaac aaactttatt gaatattgga taaatcctac
     8401 cgaattgaaa tcatatcacg tgcgaaacat cgacctcatg caatccacag gaatgaaaga
     8461 taagaatggt gtggatatat ttgaagggga tatagtatta gtcagcgtgc gaaatggctt
     8521 cgattactta gataataaag tctgtattgt caaaaattca atagattatt ccggattagt
     8581 ttgtgccact gttgatgaag acttagagta tcaaattttt aacacagagc tgtttgaaga
     8641 atacacgtac gaagtcaccg gaaatatata cgagaatagc gagttattgg aggaacagcg
     8701 atgaataaac aggaagcaat caaaaagtta gaaagtatta aagcgatagg aaatgatgca
     8761 atagctgctt gctataacga gagtataaat tcaggtatta cgttaatgaa aaaaatagac
     8821 gaaccgcaga aaccagttat tccacaactc gtggccggtt ggcttgagaa atctacggac
     8881 ccttttacaa aagctgaaaa aatagcgtat ttaatcaaat ctaaagatgg tgattcatat
     8941 tatttctgtg attggtttgt acgagatggc atagtgacgc aagagcaagg agaagaatta
     9001 ctcgcttggg caacaagaca atcatatgaa acactattga gcctatacaa cggctacgag
     9061 gttgagaaag agccgttatg ggcaataaag aatgccgatg gaaactatct tactaaatgt
     9121 gctttatggg gaaaagatgg agtaaattat agttttgaat gcaatccatc tcatcgattg
     9181 cttttcactg ataaagcaac agcggatgct gcagcattgt tggtgaatgg aacagtggaa
     9241 gaggtggtag aaagatgaaa ctaaaagacg gattttacgc tagcagtcac ggtatcggcg
     9301 gtttaatgct agatatgccg acaaagaacc ctaaaacacg caagaaatca aaattcaaag
     9361 tcggtgacat ggt
//
LOCUS       DACCXO010000106         9346 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.109, whole genome shotgun sequence.
ACCESSION   DACCXO010000106 DACCXO010000000
VERSION     DACCXO010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.109"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            94..741
                     /locus_tag="IVY70_002311"
     CDS             94..741
                     /locus_tag="IVY70_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP7959426.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            825..1769
                     /locus_tag="IVY70_002312"
     CDS             825..1769
                     /locus_tag="IVY70_002312"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP7959427.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1904..3355
                     /locus_tag="IVY70_002313"
     CDS             1904..3355
                     /locus_tag="IVY70_002313"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP7959428.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFYVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3376..4212
                     /locus_tag="IVY70_002314"
     CDS             3376..4212
                     /locus_tag="IVY70_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP7959429.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4212..4871
                     /locus_tag="IVY70_002315"
     CDS             4212..4871
                     /locus_tag="IVY70_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7959430.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            4997..5686
                     /locus_tag="IVY70_002316"
     CDS             4997..5686
                     /locus_tag="IVY70_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959431.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5692..6648
                     /locus_tag="IVY70_002317"
     CDS             5692..6648
                     /locus_tag="IVY70_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP7959432.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6692..7204
                     /locus_tag="IVY70_002318"
     CDS             6692..7204
                     /locus_tag="IVY70_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7959433.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7460..8008)
                     /locus_tag="IVY70_002319"
     CDS             complement(7460..8008)
                     /locus_tag="IVY70_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7959434.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8097..9278)
                     /locus_tag="IVY70_002320"
     CDS             complement(8097..9278)
                     /locus_tag="IVY70_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP7959435.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
BASE COUNT         3096 a         1709 c         1879 g         2662 t
ORIGIN      
        1 agtttggttc tttaattttt aaacatgaca gaaaattttt tcttcgttat acttgtaata
       61 gaatactatt ttataacaaa ggggatcgaa tcaatgaaaa aaactgttat ttatactagt
      121 ttatttgcag ctttgttttt agctgcttgt caatcaaata ccaatcaagg cacatctggg
      181 aatacgtctt ccagtagcga acagcgctct tcacaggtat cggactcctc aacaaaagca
      241 agcacatctt cgtctagcca gacaaaaagt tctagtgaag caagtactca gtcagatatc
      301 aaagtgtctg cagctcgtgc gatcgaattg ttccaagata aatacccaga tactgcaatt
      361 actagtttgg aattagatag tgattgggga tcttacttct acaagataga aggtgtcgat
      421 gatcaaaaag aatacgaagc aagaattgat gctcaaaatg aaaaaatgga gacagagaat
      481 ccagaaactc tagatcgaga tgaacaaaat ggtcaaaaga aaaaagaaga tggtctggat
      541 gttgataaat tgatcacgat tgaggaagca gcaaaaatcg ccgtcgacaa agtaggaagc
      601 ggtacggcaa cagattggga tttggataaa gatttaggta ctacttattg ggaagtcaaa
      661 gtaaaagacg gcaatcaaag tacatcagtc aagatcaata gccaaacagg cgaagtactg
      721 tcaactgaac aagatgatta agaatttctt cggaaaacaa gagttgattt ttcttttagt
      781 catgatacac tagacaaaat aacttgtttg aaggagcgat ttacttgagt ttagaaacga
      841 cctttgccaa tcatatcttt gccaatcctt tgatgaatgc atctggtgtc cactgcatga
      901 ctacccagga attagatgaa ctggctcatt cagaagcagg agcatttatc actaagagct
      961 gtacgatcaa tgaacgaaaa ggaaatcccg aaccacgcta ttttgatgtt cctttaggga
     1021 gtatcaattc tatgggactt ccaaacttag gtttttctta ttatctagag tatgctcttg
     1081 cgtatgaaaa agtgcaggaa aaccaaaatc aaccgttatt tttttccatt gccgggatga
     1141 gtgtccagga aaacttggaa atgcttgaga aaatagaaaa aagtgggttt aatgggatca
     1201 ctgaattaaa tttatcttgt cccaatgtgc ctggaaaacc acaactagct tacgattttg
     1261 aggccactta tgaaacactg aaagaagtat tcagcatctt ttctaagccg ctaggaatca
     1321 aattgcctcc ttattttgat tttgctcatt ttgatcagat ggctgatatc ttgaatcagt
     1381 ttccattgac ctatgtcaat gctatcaaca gtgtgggcaa tggtttgtat atcgatacag
     1441 aacaagaagc agtagtcatc aaaccaaaag aagggttcgg cggcattggc ggagagtata
     1501 tcaagccgac tgctttggcg aatgttcgcg ctttttatac acgtttgaaa ccagaaatcc
     1561 aaatcattgg tacaggtggg attcggacag gacaagatgc atttgaacat ctgctatgtg
     1621 gcgcaagcat gttacagatt ggtaccgagc ttcataaaga agggccggaa attttttcta
     1681 gaatcataaa agaattaacg cagattatgt cagaaaaagg atatacgtca attgacgaat
     1741 ttaaaggcaa attacgcacc atttcatagc aaagagacga aggtcatgat tgatcttcgt
     1801 ctttttttat aaaaaacact cgtctgttca ttcctgtgca agcgtactca ttgtatgtta
     1861 gacgaacttg tgggaaaata acagaggcaa aaaggaggat aatatgaatg atgcaacaga
     1921 attagcaaaa gagcttaaag aaaaagaatt tagttttcaa gaactagtta cggatataca
     1981 gaaaaaaata aagaagcaaa atcatgaatt gaatgcgttt gtcgcgcttg aaccattcga
     2041 gagtaattcc cacgagctat tgattcccca gtcagaaaaa gggccattgg ctggtatccc
     2101 atttccttta aaaatgctcg gtcaagaaaa aaagggatgg ctagctactt caggatctcg
     2161 tttatttgaa acacaccgag cttctcatac atctaattat gtgcggcaag cagaagcgat
     2221 aggtctagtt ccttttggac aaaccaatgc gccagagttt ggtttcaaaa atattacaga
     2281 cccagtcatc tatggcccag caagaaaccc ttggaacctt gaatgtacac cgggcggatc
     2341 aagtggagga gctgctgctg cagtttcatc aggaatcgtt cctttagctg gtgctagtga
     2401 tggcggtggt tctattagaa tacccgcttc gttttgcgga ttgattgggc tgaaaccttc
     2461 gcgagggacg atgccagttg gtccatatgc ttggagagga tggcaaggag ctgctattga
     2521 tttcggcttg acagtttcta tgagagacac ggaggcttta ttttatggga tgagaagtat
     2581 ccattccggt gcgccatatc aagtatcgcc agttgagtgg caaacacatc caagaaaaaa
     2641 acggttgaag atagcttttt atgttgattc accgatccac tcacagatta gtgaagaggc
     2701 agtacttgct gtaaaaaaag ctgtagattt ccttggaaaa gaagggcatg atttgacaga
     2761 gattccttac ccactagatg gtcgtaaact gatcacgtct tattatcaga tgaatggcgc
     2821 agaaacagca gcaatgatcc gctcgattga aacaggcatc cagcgagctg tcagaaaaga
     2881 agaacttgaa cccatttctt ggactttgtt ccaatatggt cagaagctct ctgccgctac
     2941 ttacatcgat tctctgcatg tatgggatga agcagcaatt gtcatggaaa acctacatca
     3001 agaatacgat ctattccttt caccaacgac tgcatttacc gcccctaaaa tcagcgaaga
     3061 tttgcaaagt gatgcgataa gagcacagat ggaacacatc agtgattgtt ctgaagtaga
     3121 aggtttagac gttgtttctt ccatgtttga ggagagcttg aagatcacac cttatacaca
     3181 gttggctaat ctgactggac aaccagcaat cagcttgcct actcatttga caaaagaaaa
     3241 catgcctcta ggaatccaat ttatggctgc cagaggaaga gaagatttgc tatttcaaat
     3301 cggctatcta tttgaagcaa atcatcagtt ccatttacca aatccatgga aatagcagaa
     3361 acaaggagaa gaagaatgaa gaaaaaaaga ttgtttgctc taataactag tgcctttttt
     3421 attttaagcg cttgcggtac agctgccgct aatcaggaag ataacagctg gagtaatgta
     3481 gaaaaaaaga aagaattagt agtagcgact tctggcacac tgtttcccgc atcttattat
     3541 aatgatcaaa atgaattagt tggttatgat gtagatttgg caaaagaggt ggctaaacgg
     3601 ttaaatgtca aaatctcctt taaagaatac aacgtggatg gacaaatcac ttctgtttca
     3661 aaaagagaat cagattttgc tgcgaacgat tttggtatca caaaagaaag agaaaaaaaa
     3721 ttctcccttt cagagacttt caaatattca tttgacagta tgatcgtacg aaaaaaagat
     3781 gattcaggaa tccactcact tgaagatctt aaagggaaaa aagcagctgg cgaacccaat
     3841 actagctata tgcgtttagc ggaaaaacac ggagctaaat tagtcacata tgacaatgcc
     3901 acgaatgatc agtatttaag cgatgtagca aatggtcgaa cagatgttat tctaaatgat
     3961 tattatctgc agaaaatggc cgtatctgct ttaccagata tccctgtaaa aatactggag
     4021 aacgtttatt tcaatgaaaa cgaaagtggt tttttattga aagaaggaaa tgatgctctg
     4081 aaaaagaaaa tcgatggcgt attaaaagag atgaaagaag atgggacgac aaagaaaata
     4141 tctgaaaaat attttcaaac tgatgtttca gtcaaaccga aagaaacgat ggctgagtcg
     4201 gtgagtgagt gatgtacttt cctctaattg atttttccct catgtggact tcacttcctt
     4261 tcgtactgca aggcttggta tacacattag ggattggctt cgtgtcgttt cttttaggaa
     4321 atctggtagg gttgcttttg acagttttag gtcttttgga ttggctgcct ctgaatgtat
     4381 ttattcgttt ttatttatcg ttctttagag gcattcccgc actagtcctg ctgtttctgt
     4441 tatattttgg gttaccttat caattaagtg ccttaacggc ttcagtcatc tgctttacga
     4501 ttaccagcag tgcatttatt ggtgaaatct atcgaggatc actagcaggc gtttcttcag
     4561 gtcagtggga cgctggttta gcttctggtt tttctttctt tggggtattg aaatatatcg
     4621 ttttgccgca agcttttcga atctcagtac cggcactcag taacgtagct atggatctag
     4681 tgaaaggtac ttcgttagca gcaacaatca cagtgccaga aatatttcaa aaagcaaaga
     4741 tcataggcgg tcggacattc gattattttt ctctctatat tttagttgca ctgatttatt
     4801 gggtcatttg tatcgggatc gaaaaagtgc aacagtattt agaaaaacgt tctatgcatc
     4861 agtcaatctg aaaaatagaa atggtgctag cgtggctggc accatttcta ttttttgttt
     4921 cttgtatatt ctgcctagta ttttaaaacc atttcatgta aaatgtaaat cgaaaatatg
     4981 ttcggaggta gaaaacatga agaccccgct aaccatcgaa atagaatcct cgttatacca
     5041 atattgcatc gagcaaggcg gcttggttgt agaagaagtc acgatgcctg atgaccaagg
     5101 aattgtagat acgcttagct gcttgacaaa atttgatgga agccgagaat ggcgatgcta
     5161 tgagctgaaa gtgacaaaag cagattttcg ctctacagcc aaactgtcat ttgttggaca
     5221 ttataattat tttgttttac caagtaaact atatgaagaa gtaaaaactg aaatccctac
     5281 aggaataggt gttttagtgt atcgtccgta cacggttgaa gaggagatgc ctgcctctgg
     5341 tacatttctg attgcgaaaa aagcagcaaa gaaagagctt gctgtaccgg aagaagcttt
     5401 gacaaaccgc ttcatggctt ctttattcag ggaagtaaga aaagcaaagc aaatggagcg
     5461 aggcgtaaaa tattttccaa cagatcaatt gtacaaagaa ttgaaaagac gttcagaaaa
     5521 tcaagatcca ttttctaacg aacgatatta tgaacggttt ttagaggaaa tagctagtca
     5581 ggcaatcaat gagttgcaag aagaagttat cgcgcttcag catgattatg aatatttgag
     5641 gcaacaacag cagatccgtc ggttgcctac agagccattg gagtgaaaaa tatgtactat
     5701 ccttatttaa gaggaaaaca atttgaccta ttagcattaa aagaagcact gtcgcgagga
     5761 ttgctctcca ataaaatcca accagtgatc gaaccagtac gtgattccgc tacgcttaaa
     5821 aatgtgatcg aattatttca aaaaaagcac catcgaatcg ctgtgatcca aaatccccaa
     5881 gtaggacaat tcaaattatt cgatcagcat gtgcacagtt ggacagtcaa agaaaatagc
     5941 tctgtcgtcc ctgcacagat cttaacgcca gaaaatctca gtgaagtact ggattcacct
     6001 ccagcgtttc ttgttttcga tggacagcac tacccaaaag atacagaggt gtggaaacag
     6061 ttggcaggag ttgattcgaa atttttgata ccagatacta gtcgctttcg catctggctg
     6121 cccgaaaaca aaattgtgat ccgtgactct tttcaaacga gaaagcatgt tgaaagttat
     6181 gcagataaaa cagatgactt ttttagtgat gactatcttt ttttccacgc agacggatat
     6241 attggatttt ctgatttcac gatcgaaggt agccgttatt tcgataaagg gttccctagc
     6301 cgagcgcttg ctatccatct aacgtatatt gatgcttacg gcaatatacg cgtgaaacat
     6361 tttgtctctg acagtaacga ctctgctaaa gaccaagcat tgaagtttct tgaagcagga
     6421 gataaaatga aaaaatggat catgcgtcac catcatcaac tcttgattac ttcaggaatg
     6481 attgaattat tggctcttta ccagcaacaa aaattccctg gactgggagt gttgaaaaag
     6541 tggtcactta tgcatcattt ggaacttatt agtcacctgc tagatcatcc aaaagattgg
     6601 cttaaatatt attcaaaagt tcaggaacta tatgaagtga tccaatgatt agataatgta
     6661 aatgaaaaag gaatagaaaa gaggaaactg gatgtatatt cggcaaacaa ggaaagaaga
     6721 agtagaagaa gtggtagaaa tcattaatgc cgcagcggat ttcattgctt cgcaaggctc
     6781 tcctcaatgg caaaacgggc agaaaccaac aaaagaaacg attttgaacg atattgaaag
     6841 aaaagaaagc tatgtattaa tagtggaaga tcaaattgct ggaacagctg cattagtctc
     6901 aagcgtggat cccgtttaca caaaaataga tggtagttgg aaagaatcaa ctggtcctta
     6961 tttatcgatt catcgtgtcg ctttagcaga aagtactcga ggaaaaaaac tagcaaaacc
     7021 gttcttagaa catttgttga cgattgcagc cagcaaaaat atccaagata tccgtatcga
     7081 tacttatcca gctaatgaac cgatggaaaa gaccatctat tctgctggat tcacttatca
     7141 agggatgatt gaatttcctt ttgaacacgg cgaaagaaaa gcatatcaaa taatcatcaa
     7201 ttgactataa aatcatctga gcaaagataa agacattaac actactgaaa aaaacaatca
     7261 tatgatagcc aagaggttgt aacagaagtg gccagcttca aaaaacaaga aagaatttcc
     7321 gaaaatgctt ctcaaatttt tgtggaattt tagcgtattt tacgaaatag ctgctttctg
     7381 aaactccctt catcgagagg tcgtgacata gcttttgtca cgacctctcg gcttatccta
     7441 cgagtattat tcgatacatt tatatcaata atcctttttt tattttatct gcagcttctt
     7501 ggaacgctcc atcgtttgac acgactaaaa gtgaggggat agctttcaaa cgtttcaact
     7561 gtgctaaatc ttctacataa accgccagtc tggctgaaat ctgttcagat gaatctccac
     7621 gttttttcag gcgcgattgc aatgtttctt tagagatcgt aataaagata ggacggacta
     7681 ccgtcccgaa atactcatat aaatgccaaa aaccatttgc tgtcaaaacg gcgtaacaat
     7741 ttcctactgt cagtttcttt ttaatttctg ctttactgga accatattta tgttgagcat
     7801 attggtccca ctctgcaaac tcatttccct tgatcatctg attgaatgta ttgtcagaaa
     7861 caaaataata atcctcttga tctttttccc caggtcttgg tgatcgattc gtgtaagtaa
     7921 tgatcttttg ttccggctga aaaatagctt ctgcgagtga agtttttcca gaaccagaag
     7981 gaccgataaa tacatagcaa taattcattg tcatttctcc tttgatgatt gaacaagtga
     8041 aaaagctttg gaaaatattt ccaaagcttt taaaagtaac tttgcttttt tgtatattat
     8101 accagcaagc cagtcacaat agcacttaaa aagctgacta aagtcgctgt tagcaaaatt
     8161 ttcaatccgt gttttgcgac aagatttcct ttttcttcat ttaatccctt catcgcacca
     8221 gaaatgatcc cgatagaaga gaagttggca aatgaaacga ggaagacaga tataattgct
     8281 gttgtacgct cagtaaaatg gaaattacca tttgctaatt ctgtcatagc aacaaattca
     8341 tttgtaacca gtttagtcgc catgatacta ccggcatcca ctgcttcttt ccatgggatt
     8401 ccgctgatga aggcaagagg ggcaaagatg taacctagga tctcttggaa agtgatgccg
     8461 aatatcccgt ggaagatagc attgatcatg gcaatcaaag caacaaaacc gataagcata
     8521 gcagcaaccg tgatcgccac atggaaacca tcaagaatgt attctcccaa catttggaaa
     8581 aatgtctgtt ctttgttttc ttcaatgatc agctcatctt ctttttcttc taatacatag
     8641 ggatttacaa ttgaagccag aatataaccg ccaaataagt tcaaaacaag ggcagtgatc
     8701 acatatttag aatcgatcag tgccatataa gatccgacga tcgacataga aactgtagac
     8761 atagccgata cactcatggt gaacaaacgt ttctcagata ggaagggtaa ttcctttttt
     8821 atggaaataa aaacttccga ttgtcctaaa atagcagaag ctactccatt ataagattcc
     8881 aatttgccca tcccgttgat tttacttaat cctagaccaa cagctttcat aaataatgga
     8941 agaatcttga tatagcgtag aataccgata atagcagaaa taaatacaat tggcatcaat
     9001 acggaaagaa agaaaggtgc acttccttta tttgctacac cggcaaaaac aaagtcaaca
     9061 ccttgcccag caaaagcaag aagatgatca aacatttttg ccaatacaga aacaaccgct
     9121 gtaccaaaag ttgtacgtaa taatacgaaa ccaagaacaa gctgtaaaac gaacatcaca
     9181 attagtggtt tcaatttgat tgcttttttg ttactgctga tcaaaaaagc aagccctaat
     9241 gtgacaaata aaccaataat acctaataaa taactcaaat aatcactcct attccaaatt
     9301 ccgttttatt ttacctgttt tgcaaataaa atactaatct ttagtg
//
LOCUS       DACCXO010000107         9300 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.110, whole genome shotgun sequence.
ACCESSION   DACCXO010000107 DACCXO010000000
VERSION     DACCXO010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.110"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(314..1627)
                     /locus_tag="IVY70_002321"
     CDS             complement(314..1627)
                     /locus_tag="IVY70_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP7959436.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(1872..2096)
                     /locus_tag="IVY70_002322"
     CDS             complement(1872..2096)
                     /locus_tag="IVY70_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959437.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(2116..2484)
                     /locus_tag="IVY70_002323"
     CDS             complement(2116..2484)
                     /locus_tag="IVY70_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7959438.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(2680..3390)
                     /locus_tag="IVY70_002324"
     CDS             complement(2680..3390)
                     /locus_tag="IVY70_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959439.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(3408..3905)
                     /locus_tag="IVY70_002325"
     CDS             complement(3408..3905)
                     /locus_tag="IVY70_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7959440.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(3942..4394)
                     /locus_tag="IVY70_002326"
     CDS             complement(3942..4394)
                     /locus_tag="IVY70_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7959441.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(4529..5134)
                     /locus_tag="IVY70_002327"
     CDS             complement(4529..5134)
                     /locus_tag="IVY70_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP7959442.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(5584..6204)
                     /locus_tag="IVY70_002328"
     CDS             complement(5584..6204)
                     /locus_tag="IVY70_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7959443.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(6206..7075)
                     /locus_tag="IVY70_002329"
     CDS             complement(6206..7075)
                     /locus_tag="IVY70_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP7959444.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(7098..7505)
                     /locus_tag="IVY70_002330"
     CDS             complement(7098..7505)
                     /locus_tag="IVY70_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP7959445.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(7524..8813)
                     /locus_tag="IVY70_002331"
     CDS             complement(7524..8813)
                     /locus_tag="IVY70_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7959446.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            8871..>9300
                     /locus_tag="IVY70_002332"
     CDS             8871..>9300
                     /locus_tag="IVY70_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7959447.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT         2705 a         1942 c         1564 g         3089 t
ORIGIN      
        1 aaatggatgt ttctattttt tgcttaatca tttattttag tctctctcac taaaaattgt
       61 aacgctttca aatagctatt gcaccttgtt atcggtaaca gaataaaaca aaaaaacagc
      121 tgaggaaatt ccctcaactg ttttttgaaa ggattgattt atgcagggaa gaatgctgat
      181 ttcacaactt ctttataaac ggtgttcact cagctgtccc ttggtattag ctcaaaaaac
      241 ggcaaaatat aagaaactat tttttcgttt ttttctctaa tactcaggcc agaacgctga
      301 tttcacaacc tctttatttc acattcaata tccacatgaa tcccacaaat acaaagaaca
      361 gaccatacat gattggagat acttcttttc ctcttttagc tgctaacatg gtaattggat
      421 agaagataaa acctaatgca atcccatctg aaatactata agtcaaaggc atgccgatca
      481 agatcaagaa tgaagggatc gcaatctcta agtttcccca ttcgatttgt cgcaaagatt
      541 gtgccatcaa tactcctacc ataatcaatg ctggtgcagt tacttgtgaa gtaacaactg
      601 atagtaatgg agaaaagaac aagcctaaca agaagaaaat ccctgtagta atagctgtga
      661 tacctgaacg tcctccaacg gcgataccag ctgatgattc tacaaatgca ccaacaggag
      721 aagtaccaaa taaggaaccc gcaagcatag ctgttgaatc agaagctaaa gctttcccta
      781 cacgaggcat cttgttgtct ttcatgaaac ctgcttggtt tgctaatcca actaaagttc
      841 ctgctgtatc aaagaaagtc acaagcaaga atgtcagcac tacgacccac atttgaagag
      901 aattgatgtc ccctacatgt tttaatgcaa ctaaaaatgt tggtttcaaa cttggtgctg
      961 cggcaacaat ttcagaaggc attggaatca aacctgtgac cagacctaaa atcgttgtag
     1021 ctgccatccc gatgaagatg ccacctggta cacgccgaac aagcaagata gcagttacga
     1081 ctaatccaaa aatcgtcaac caagtagaac caacgttgag tgatcctaat cctactaatg
     1141 tagattcatt cgaaacgatg atcccacctt cgctcagacc aaggaaagcg ataaatagac
     1201 cgataccgcc agaaatcgca tatttcaaat ctgctgggat cgcatcaatg atcatttccc
     1261 gcagcttgaa gattgtgatc aaaataaaaa tcaaagaagc aacgaatacg ccggctaatg
     1321 ctgtttccca tggaatcccc attcctaaac aaacagaata agcaaaaaaa gcattgatcc
     1381 caagagcagg tgccgttcca attggataac gggctaatac ccccattaaa acacagccga
     1441 tcgcactggc taatgcagtc gcagtaaaca ctgctccttc atccattcca gaagcaccta
     1501 atacagttgg atttacaaat aagatataag ccattgagat aaatgttgta aatcctgcca
     1561 gtacctcacg ttttaccgtg gtgttcaatt tttctatttc gaagtaggaa agtatttttt
     1621 ccttcatctt ccatccccct aaatccgaac attataaata aagtccgtta aaatttcgat
     1681 ttattaataa aacaacgtgt tttatcatat actaaaacaa aacataagta aacaaaaaat
     1741 tcggatttta agcaaaatcc gaacgttcca tctgattaaa tccctttatc tcaaagttca
     1801 tgaacattca ctttggatta caagaatttc tttattgtgt taataaagtt tcttcattct
     1861 agtctcaaaa atcaatagtg tgatttgatt ttaccatttc gtttctgttc ttctttgatg
     1921 aattcttttg tatcgacagg attcagatta tttagtccgc caaaacctgt atcgaccatt
     1981 tttccccagc cttttgtctt cttttttcct gatttgagat tctttgctcg aagcttctca
     2041 ctatgttttg ctcgccgata cttcaaataa ccttctaatc ttcccattag atccactgtt
     2101 tttcctcctt tttattcaat cagacgactc agcttttcct ataacaaaat aattgccttc
     2161 agggtcagaa aaattgaaag tcaagatgcc attgttatct gtgatttctc ctggttcttc
     2221 catgttctcg tagatagttt caaattcatc cgtaaaaaat actaaagaag gcggtggtcc
     2281 taatacctct ggtgaaaact tctgcacaaa tacttttggg aatacgccta attctaattc
     2341 ttttgagatg ctgagtacaa tactcttaaa ttcttctggc aattcgattg tttcagaaat
     2401 ttctgcacca attttttcta caaagaatcg gcaaatcatc tctacgtcat tgacataaag
     2461 catcacacgt gttttttgta tcatcatttg acccttcttt ctatttgatt ttctttcctg
     2521 tctataaact gattccatta tatcacgaaa gcgcaaacac cttgtgtttg atactattca
     2581 agaaaaataa agcaatagtt tctaggattg ctttcctcca caaacttttt ttacaaaaaa
     2641 aaaacggcaa tgaatgccgc tcttcttatt tcgattcttt tataattctg cttctgattc
     2701 atctttggtt agtgtgtgtc taacactagt tacgccaaat gtttgatcga ttttttcctt
     2761 caacgcttcg attgcctgtg cgccacgtgc cgcttgttca gcattgatgg attcaatgac
     2821 cagcaccgct gcttgtgttt cttccgtcag catcgttctt tgggcttcac gccagttgag
     2881 acagcgacag atcgctcctt gttcatcata gtagatgatt tcttccggaa gtgccggagc
     2941 atcggaatct gcacctaaag gcaggaatga ttcgccacct tgggcttttc ctaaatgcag
     3001 atttcctgcg atcttttcga tatcttctcc gccgcaaggt acgccaaaag acaaggaaat
     3061 actattatag atatctacaa gcggattgat tgggaaaaat tcccgttctt gatttgcacg
     3121 tttcagtaaa gcttcaatag aagaacgtgc acctttttta gtcttgaatg tggtaaatgc
     3181 ctgacgccac tgagcaatga cttcgttttg gctgaatgtt tcttctgtaa ggtaatttct
     3241 tgcttctttt gctgcctcat tcaataattg atgatacgtt tcttcacttt cttttgttac
     3301 gtggttatcg ataccattta cggataagat ttcgatggtt gcttcaggaa atacttccca
     3361 gaaattggta tccactatta cttttttcat ctgttcacac ctcttgatta gaataattgc
     3421 ttgatgaata gatagtctgt ttggggatca tctcccatat aaaaaacatg ttcgctgtat
     3481 ttgataaatc cttttttacg ataaaaagcg agcgcaacat cgttgtgttc ccaaacaccg
     3541 agccaaatgg ctgttttttg cttttctttt gctttttcaa tagctgtatc tagtaaaaat
     3601 gtaccgatac cttgatgttt aaattttttt cgtacataga ttcgctcgat ttctaaaaaa
     3661 tgatcagcca tttcctcaga ttgactcgtt cctgtattta gtttcaggta acccgcaggc
     3721 tcttgatcga tactcactag gaaaaaatcg gtttctttat cttttagctc ttctgtaagt
     3781 ttttctagtg cataagcttg tgtcaagtaa tcagctaaat gctccggtga attatctttt
     3841 ccaaatgtat ctgtaaatgt ttcgatacta atttgttgta gtaagtctac atctgaaggc
     3901 gtcatttttc gaatttcgta tttattcacg gcttcttcct ttcaatagat ccttttattg
     3961 ccttttttga caaaattcca atcttcgcta acattcttgc atactttatt taaaagatat
     4021 tccaattgtt ttgcttcttc gtcagaaaga ccatttaatg cgactgcatt ggagtagttg
     4081 ttttctcgaa tgatgaaagg atagatttct gctccttttt tagtcggata gagtttcttg
     4141 atttttttat tttctttatc ttccaaacgt tcgatcatcc cattggattc taatttcttg
     4201 attgctcggg cagtcgttgt acgatctact ttgatcattt ctgctagttt ttcttggatg
     4261 attccaggat tttcgcaaat acgtactaaa taaagatact gtcctcttgt taaatcgact
     4321 tctttgaatt cgatattgct aatagaatct agcgctcggg caatcattcc gatgtctcga
     4381 aggatttcag ccatttcatc ccacctttat tttgtttgta agactagttc acctcttttt
     4441 gttgcaaatg caactatttt caataaaaaa atcaagttct tttttaataa ctagtaaagt
     4501 atattctttt cttcttttac gaaaaagctc aatcacaata gatctggttt ttgttttcta
     4561 aaataagtaa ctgtttcttc gttgttcgct tttctgatag ctttggcaac tcttctatac
     4621 taaaaaatcc catgttggaa gtttcattat tctcaataaa cttggcctct tcaccgtgga
     4681 tcgcacaagc aaaaacaagt ttataatact gaaaaagctg tggaatatct ttttgtttat
     4741 tcgtatcaaa aactgctcgc aactgagtag tttctactgt taatcctgtt tcttcataga
     4801 cttctttacg cacattttct tcaggagaca atcctatttc agcaaatcca ccaggaagtg
     4861 accattcttt cgtccgttta tcttcgatta gtaggacctt gccatctttt ttgatatagg
     4921 cacgaacatc tacttttggt gttggatgtc cttctccaat cgttgtgagc gcttttattt
     4981 cttcgaaagt ttcttttcct atttcactga tcagttccaa tgaaatcgtc cggatctcct
     5041 caaaacgctc tttatcaaat acatcttttc cataatatag accagcttga gaaagtgcga
     5101 gtagtctttt gtattgagcc aacttcttgt tcattctctt cccttctttt ttagcaaatg
     5161 tatgactatg taatcaagta tagaagactg gtttaattac ggcaaataaa tataatgaaa
     5221 tctgcaataa ataaaagaat gttaaaaaac tcgagaacaa aagacattcg acttctctgg
     5281 ggctgtgaat aaatggcgtt caaaagcttg catctgcggt aataagcccc accaaacgaa
     5341 aaatatgacg agctattctt gtctggctgt tcttattaat tgaggcagga cagctttttc
     5401 acaacctccc cactaaacgg tgttctccta ccaactcctc ggtcacaagt cacaatattc
     5461 atcaaacatg agaagctgtt tttttgaaat tgctccttgt gactcggagc tgaacgacag
     5521 gctcacgacc tctcggtaaa cagcgttcaa aagccaattt ttcgatatta agccatttct
     5581 ttgttactta ttgtcattga tttctttttc taatttatcc aatagtggag tgtaatccac
     5641 ttgagtatag tctgttacat caaattcata tagtgttcct aactggaatt tgtcatattt
     5701 tcggtcggcg atgatctttt tgaatgcttc tttcgtaata tgattggtcc ctttcgttcg
     5761 atcttccaat acgtcaccat agtgataatg atccatgata tagctcaaat gacgatctga
     5821 ttcacgatca cgtttgtacc gacgttgcca taacacttca aagtctgctg ttaggcgaat
     5881 cgtgatcacc tgatatccgt atctatctgc taattcttgg agtcgtgctt tttgcttatc
     5941 actaaaagga tactcggaca caatcacttt ctttcctgct tccatataca aatcaagtgc
     6001 tttgtaatag aaaggataaa ctttcgtcct ttcttgttcc gcccgttctt ccaaattgtt
     6061 aaacccgatg gattctgcat agtattcctt gatttcatcg agcgtgatgg catacatatc
     6121 tggaaatcgt tcccgtagtt tattcataag atatgttttt cctgtaccag gactaccagc
     6181 cagaagaatg aaatactttt tcatctcatt tatttcctaa gttaattgga ctttccaagt
     6241 tcataaacag tccaatctta tgcttgacaa tttcttttac tgcttcattt gctggaatca
     6301 cattatggcg aagagacttg ttgatttctg tttcactaaa ggtttcttta gctactttcg
     6361 tccagttgac taaaagctcc gttccaacat tgaatttgcg gatcccgtta ttgatcaatt
     6421 caggataatc ttcttctttc acacctgttc ctccatggat aactaggggg acatcaacta
     6481 ctgcatcgat ttctttcagc aagggaatgt ttacttctgt ttttgatttg aattgtccat
     6541 gattcgtacc gatagcaata gctaatgcat ccacgcctgt agcttctaca aatttcactg
     6601 cgtcttccgg tcttgtatat accttgtcat tttcagcgac atggattcct tcttctgtac
     6661 cgccgattgt tccaagttct ccttctacgg aaacaccgtg cgcatgggca tattctacta
     6721 ctgcttttgt cttcaggata ttttctttga aaggcagatg ggacccatca tacatgacag
     6781 aagtatatcc gctgtcgatt gcttctttga tatcatcaaa gtctcgggcg tggtctaagt
     6841 gcaagactgc atcaaccatt tcttcttctg caatagctgt cattacattg actaacacat
     6901 gcatgccgat atatttagcg gtatcgacac ttgtttgaat aatgatcggc gaacccattt
     6961 cttttgctgc gcctagcata gcaggaagca tttctaggtt atgcgtatta aaagctccaa
     7021 ctgtaaaatt caactcttct gctgtttttg ttacttcttt caatgttgta tacatctttt
     7081 attgccacct tatcttttta tttgtttcct ttgatcgtta cttgttttgc aatttgtcgc
     7141 atctcatcca ttttgcccat gtagtaatcg atcccttgat catcaccagc acgagcagca
     7201 tctgcacgct tctggttata gaggctcatc agttttttat acccatctcc gatcgtctgt
     7261 ttattttcca aactcttttc taaatattgg atggcttgat cgttcacata caattcagaa
     7321 aatttaagtc caacttgttc ataaagtttc gctaatttat gcttatcttg ggtagtggaa
     7381 atctcttgtt tcaattgtgt aatctctgat tgaatctttt cggtcgcttc tggcgatagg
     7441 gctttttctt cttttgccag aacttttttc ggttcttttt cttttttctt aaaaaatcca
     7501 aacatcattt tccctacttt caattaaaat gctttcaaaa ctgggcttaa gatccaggca
     7561 tataagagtg ctgcggctac tgtatctaca tcggttgctg tagcattgtt gaatcccatc
     7621 gaattaaaga tcgttactag caaagcgggc aataatgtaa taaagaaacc atgagcaata
     7681 ccgccaatga tcgctcctcg gcgtccgcca actgcgttgc caaatatccc tgctgtccct
     7741 ccggcaaaga aattggttag cataccaggt aaaatcatcg ctaagccgaa catgggcaaa
     7801 gtaaacatgg caataatcgt accaatcgtt gtcgtaatga aacctaaaat gacagcattt
     7861 gggctataag gaaagaaaac tggacaatct aaggcaggga tcgcatcagg aactaatttc
     7921 atagctatcc ctcgaaatgc cggcacgatt tcacctaaca gtagacgtac accagctagc
     7981 aatacataca cacccactac gaattggatg gcttgtaaaa aggcaaacat gatatagttt
     8041 tgatctcctg ataattcaga agcgaatggt tcgcctgcaa ataaaacagt gatgatatat
     8101 agcgggacca tcacgaccat gacagacaaa taagtatctt gcagaaattc gaaatgtgtc
     8161 ggcaacttga tatcttcgat cgacttttta ttttcgcctt tctcaccaaa aagcttagca
     8221 acaccggctt caaataagta cccgatcgta caaaaatgac ctaaagcaat atcatttgat
     8281 ccagtgattt tacgaatgat cggttgcgct actgccggca tagcaatggc gaaacaagct
     8341 ccgataaaac caccgaccaa gatcaatacg atccctcgca gaccggcaaa atagccaaag
     8401 actgtcgtca tcgttgccat ccataaaatt gcttgaccag ttaaaaaaat gtacttccat
     8461 tttgtgaaac gagccaaaat aatattgaat acaaaaatgg ctaagaacgt cagtgcaata
     8521 tctctaccta aacccaaatc attcattgct tgaccattga tagattcaat agaagggatg
     8581 atcccttgca tatgaaatcc ttcagtaaag attttcccga aataagttaa acttccaacg
     8641 atgatgctcg aaccggcact caacacttga aatcctagga gtgtctttaa cgtgccagag
     8701 atgacttgtc ctgctgattt cttctgcagt agtagaccca acatagcaat caatgcaatg
     8761 gtgatcgatg cctgagtcaa aatattttcg atgataaaat tgataacgcc catgttttga
     8821 tgtgcacccc aaaagttaga ctagaaatct aattaaaagg ggtgcttttt ttgcggaaat
     8881 atacatttgt ttttaagaaa aaagtagttt cagactattt aaataacgaa ggcggctaca
     8941 aatatcttgc acataaatat caaataaatc gtacactggt tagacattgg gtaaggattt
     9001 ataactatca tggttgggaa ggcttggttg gaggtggcaa aagctacact acaaaattta
     9061 aacttgatgt tatagaatat atggaaacaa atggtctttc tatccaagaa actgctaaaa
     9121 aatttaatat cggttcaaat agaactctaa gtaaatggat agagcaatat gaagaaggcg
     9181 gtgcttcttc acttgagagc caaaaaaggg gcagaaaaat tagtatgaat tccaagctaa
     9241 acattcctaa aaaacttaaa gatgagtctc ttgaagaaga agttattcgt ttaagagcag
//
LOCUS       DACCXO010000108         8990 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.111, whole genome shotgun sequence.
ACCESSION   DACCXO010000108 DACCXO010000000
VERSION     DACCXO010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.111"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            143..1357
                     /locus_tag="IVY70_002333"
                     /pseudo
     CDS             143..1357
                     /locus_tag="IVY70_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1536..1871)
                     /locus_tag="IVY70_002334"
     CDS             complement(1536..1871)
                     /locus_tag="IVY70_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7959448.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1855..2127)
                     /locus_tag="IVY70_002335"
     CDS             complement(1855..2127)
                     /locus_tag="IVY70_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP7959449.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2578..3381)
                     /locus_tag="IVY70_002336"
     CDS             complement(2578..3381)
                     /locus_tag="IVY70_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP7959450.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3359..4483)
                     /locus_tag="IVY70_002337"
     CDS             complement(3359..4483)
                     /locus_tag="IVY70_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7959451.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4499..5314)
                     /locus_tag="IVY70_002338"
     CDS             complement(4499..5314)
                     /locus_tag="IVY70_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7959452.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5304..6185)
                     /locus_tag="IVY70_002339"
     CDS             complement(5304..6185)
                     /locus_tag="IVY70_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7959453.1"
                     /translation="MSEMKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAP
                     TKEFVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNL
                     YKPLFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAG
                     ITAWKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVM
                     TIVQGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAV
                     FLGVIFKFVEKGVYYEN"
     gene            complement(6261..7550)
                     /locus_tag="IVY70_002340"
     CDS             complement(6261..7550)
                     /locus_tag="IVY70_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7959454.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7778..8422)
                     /locus_tag="IVY70_002341"
                     /pseudo
     CDS             complement(7778..8422)
                     /locus_tag="IVY70_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2791 a         1910 c         1434 g         2855 t
ORIGIN      
        1 ctctttttga gttgaaacga agaaaaagtg ttagtagaaa atcgccttcg attttccacc
       61 aacacaaaat attatagaag caaaatcaat tctaccttag atgttcttat atcactctat
      121 cagtcctatt tgacaaagag ccaagaaaaa gacgttggtc gtcgatgctg ttctctcgcc
      181 tactcctcgt gcttgtagaa gttgtggttc tactgtggta gatggaaacg ggaaagcaat
      241 tatagtgaaa aatgggaaga aggaaacgat tgtccgtttt gaacaataca atcatatgcc
      301 tttggttacg cgcctaaaaa agcaacgcta tacctgtaaa aactgtcgta cccattggac
      361 ggctcaaagt tattttgtcc gactcagcca ttcaatcgca gatcatgttc gatataaaat
      421 tgcttcttta ctgactgaaa aagtatcttt gtcttttatt gcgaaaagtt gtcaggtatc
      481 tttgactacc gttactcgta cattgaaaga gtttaaaagc tatttaccaa agccttctaa
      541 gaagattctc cccagagtat tgatggttga tgaatttcgt tcgcacgctt ccatagaaga
      601 taaaatgagc tttatttgcg cagatggtga aacaggaaac ttaattgctg tcttgcctac
      661 gcgtaaatta cctcgattaa caagctattt cttaggctgc accaatccag aagaagtaga
      721 attcttagtc acagacatga acgccgctta cttccagctt accaaacgtg ttctgccaaa
      781 tgcgaaagta gtgattgatc ggtttcatat tgtcaaacac atgaatcagg cactcaatga
      841 gttgcgtatc cgtgaaatga atgagcttcg taaagcagga cagaaaagtc aggcagaaaa
      901 actgaaaaag aactggcgct ttctgctaaa aaatcgtgca agcatcaacc attatgagta
      961 caaaacatgg aaaagtttcc gagcaccaaa atacccattt ctcactgaag caatgatgat
     1021 tgatcgattg cttgagtttt ctgcgcctct aaaggaggcg tatccctttt ttcatgaatt
     1081 agtcgaagcc tttcgagaca aagaccctga cttatttttc tccttattgg cagaacttcc
     1141 cgaaacgtta gatgacggct ttcgggagaa gcttcaaaac cttctgacct atgaagaagg
     1201 tatcaccagc gcaatgatct atccttattc caatggaaaa attgaagcga agaataccca
     1261 cataaagaca atgaaacgag tatcctacgg atttaaatca ttcgagaaca tgagaattag
     1321 aatctttttg atcaatcaat taatcaatgt aagataacaa aaaatctgaa ccagaatgtg
     1381 gttcattctg acccggattt gattttgcac aactcatcag tccttattga caaagagcca
     1441 aaaatctgag ccagaatgag gttcattctg acccagattt gattttgtac aacccatcag
     1501 tccttattga caaagagccc ttctttttag tattattata attcttccat gcgttcttcc
     1561 agtcgctgta aatcgcttgg acttaattcc ccaatttttt tccatttgac ctttttgttg
     1621 tttttgggta attcgtttag agttcctacg tcaatccaag attctttttt tagtcctgcc
     1681 tgtttccaat cccttatttc aacgtatttc agctttattt tatcggattt agtatgatat
     1741 tttgaagtaa ttgacagaaa cgaaattgtg tccagtccat tttttagaat atagacaggc
     1801 cggcgtttac cgccctctgc agctgtgaaa gcaacgtaga cggttgctat atcattagta
     1861 ttcatcttca ttgaaccatt cttcaatttt ttcgtcagtg tctagttttt ggacaggcaa
     1921 tttttccgct aatctttgaa tattatggat tcgtttactt ttttccgaca attcaataga
     1981 aaatgggatt tttccttgct caacaatttg cttataaaaa acggtgacgg ctgttttggg
     2041 gctcatgcct agttcatcaa taatgctttc tgcttcattt ttcaattttt cgtccataga
     2101 aatttgtaac ttagccattt tctccacctc tttctgacaa acacattata tcacggaaag
     2161 agttcaaatg gaattaatac aactactttt gaactccttg aaatgcagtg tatttatcta
     2221 aatataagga aaggatatag tgctaaatgg ccttgataga tagaaaagtt tcaaaatccg
     2281 ccacagcgat aattgaagcg aacactcaac gacacacaga acaggaaaag actcctaaaa
     2341 gaaccaaatt aactaggacg tgtctgaaaa ctccacaaag atgtggtatg ctctggtaaa
     2401 aaacgaatgg cacaaccacg aagaccgata tggttcttgg aaaactttct ccaccctttt
     2461 tgtaattgga gggatagtgg tttattgata gcgattttcc aagttcttca agtggaacca
     2521 gacattgagc accacggcta aaaagtgaat gaagtcttta tttttaatca ataattttta
     2581 atgattacca gtaattggtt ttttcatgcg ccctatttct atttgatgtg gagttaaata
     2641 atgctgataa gtaaaaattt ttccgtcaat taaactgcca ctagtgaatc ctgttctatt
     2701 ggtaaaaaga gaatagtctg ggtattcatc tacaccaaat gataaagatt cagccataaa
     2761 ctgtttttta ccagcaaatt cagcactgct accttgatgg atatgtccta caaaaatacc
     2821 taaaatatca ctttcggcaa tcaattcagc aaaaccttcc ggaacttcag ttacaataga
     2881 tggatcttcc cacgacaatg gatgatgaac caataaaatc gttccctttt cagcaggtgt
     2941 acccaaccac tcttgtaatt gaatcatctg tcctatcgat atttttcctt cgtgctgggt
     3001 atcttgagca gaatccaagg aaataattcg taacccattt tgattcgtac aatggatata
     3061 ctctcccgtt tcattgcttt caacactcat tccttcaaag aaaacactgc gacgatcatg
     3121 attcccacga caaaaatagt aaggtgtggt tgggaaatat tgatccaaca ttttccgtaa
     3181 gacttgataa tcagaaatcc ccccttcatg tactaaatca cctgttaata gaataaaatc
     3241 gtacactgtt aacgagagtt cttgtaacaa tttttccaat gccaactgtg gatttttaat
     3301 tgttggaaat agttcagcag taaacaagtt agtttgatag ttttctctaa aatggatatc
     3361 agagagatgc aaaaagcgca tggctattcc ctctttcttc aataaaataa tcaggatatc
     3421 ctaaattttc ttgcgtctga taatcaaaaa aatgcattct ttcaggttct atgcggagat
     3481 tgactgcatc accaatctgc caagtaggtt tttcattaag gacaatcatt tctataccat
     3541 cgactttaac ggtgtacgca taactttgac ctaatagctc acgatacttc acttgaccgt
     3601 gtaaatcgct tgtttctttt gaaaaggtta cgtgttctgg tcttatgcct aaatacaatg
     3661 agccactaac ctgaagaagt tgtttttgcc aagctggatt caatgttaaa aattgactgg
     3721 ctacctttaa tgttccttga tcataagtaa ctggcataat attcatgcct ggcgaaccaa
     3781 taaaatttgc agtaaaagta tttttaggtt tcgtataaac atttttcggc gtatcaatca
     3841 tctgaattct accttcattc aataaaacaa tccggtcagc taacgtcatc gcttcaattt
     3901 gatcatgagt gacgtagaca aaagtttgtt tataacgttg atgaattttt accaattctt
     3961 ttcgtgcatc caatcgtaaa cgcacatcca aatttgataa cggttcatct aataaaaaat
     4021 aattactccg tttaaccacc gcacgagcca atgctacacg ctgccgttgc cctcctgaca
     4081 aatctttagg aagtcgatct tggtatggcg ttaaacctaa catcttcaaa gcttcttcca
     4141 aacggtgctg aatgtcagct gtcttaattt tattcgccct taatgcaaaa ataatattct
     4201 cctttacagt catgtgagga tataaagcat aattttgaaa aaccattgcg atctctcgtt
     4261 ctccacaagg tagcttattt gctagttgtg tacctaaaaa aagctctcca ctcgtaattt
     4321 tttccaaacc agcaatcatt ctcagcaaag tagattttcc tgaaccagat ggtcccagta
     4381 acacaattcg ttctccagcg gccacagcta aatcaatttc ttttaaaatg gtatgttctc
     4441 catatttttt tccgatattt cttaattcaa gcgtttctcc catgatttgt cctccaattt
     4501 atttaatacc tgcagaaaca aaagtcccga taatatattt ttgaaaaatt gcatacaaga
     4561 ctaacggtac aaacattgta attaaactaa tcgccattgc tacggtaaaa tttgtcccac
     4621 cttcagaact aatataggtc tgcatcgcca acggcaaagt ataattatct actgtccgta
     4681 aaattaatac cggccaaaca tactcattcc aagaggtaat aaaaaaccaa ataccggtag
     4741 aagtaatcgc atgtctagta agaggaaaaa caattttact catgacggtt gtttgtttga
     4801 tgttatccaa tgccgccaca tctagaaggc tcactggaat ttcccgcatt gtttgcgtca
     4861 ataaaaaaat gcccatcgca tcagcaaatt gtggtattat cacaccccaa gtagtatcaa
     4921 ttaaattaag ttttgcaatc aacaaataat taggaatcat cgcaacagta aaaggaatga
     4981 acatcgtacc aatgatgata aagtaccaga ttttcttccc tttgattcgg caataagtaa
     5041 aactatatgc cgcaaaaaaa gctacgccta acttaacaac cgttataatt gtagcaataa
     5101 aaaaagtatt tctcgtaatt ttgataattg gaagattttc gaacaaatag caaaaatttt
     5161 ccaaggtaaa cggctgtggt atcaaactag taatcgtctg ataggcatct ttcaatggct
     5221 taaaagaatt ggccataata aacaataatg gatagataat aatgattatg atgaacataa
     5281 ataagagatg tggcaaccag cgtttaattt tcatagtaaa cccccttttc cacaaattta
     5341 aatattaccc ctaagaaaac agcaaatatt aataaagtta aaactgaaat tgctgaagat
     5401 tctccgactt tatacatttc aaaagccttt tgataagcat aatagatcac gtttgcacta
     5461 ccattattcg gtccaccacg agtgatgatc ttaatcggag taaacacata ctgcagacct
     5521 tgaacaatgg tcatcaccaa taaatatagt cctgtcgaac tactcatagg caacacaatt
     5581 gtaaaaaaaa tgcgccagat gggtacacga tccaacttag ctgcttcgat catctcttga
     5641 tcaattccca caattgctgc aaaaatgaca atgaaattat aaccaaaaac tttccaagca
     5701 gtaatacctg caatcacagc aattacccaa ccttgtaaat tcatccacat aggcagtctt
     5761 aaaccaaaat ttcccaacaa gacagcaatt ggtcccgata ccggatttaa tatccatgta
     5821 aaaaccatcc ccgccactac caaagaaatc acagctggaa taaaaaataa tggtttatac
     5881 aaattctgcc ctttttctat aactattcct aataaaaagg atgtaatata tgggaacaca
     5941 cagctcagca agacaaatag aacggtatag gacaaagtat tcttcaacac tttatgaaag
     6001 actggatcag tcagcacctt tacatagttg gcaaagccaa caaattcttt agtgggggcc
     6061 accatattcc agtcaaaaaa actcaaatag atcgtataaa tcaatggata aaaaacaaac
     6121 acgcccaaca aaataattgc tggcgccaga tacaaataac ctacatagcg cttcatttcc
     6181 gacagcctcc tcatccttat gtcaaaagca gacgaccttg tttttccttt cttacaggcg
     6241 ctgtcgtctg cttgctttgc ttatttgagt aactcattaa tttccttttg agtagctttc
     6301 atcgtttcat tgacggaatt acctgataaa attttatccc gcatatccaa caatttttgt
     6361 tcagcttcta atccagcatc tccaggaaat gaactccaac tgaccatctt atccatctga
     6421 tcaattgctg gtatcatcat ctgattttca gctaagaaag cttttaatcc attttgggcg
     6481 tctgcaacat ctttacgtgg tggtaaataa ccagtacctt ctgtccattt agctaaagat
     6541 tcaacactat aaagatattt taagaaatcc caagttgctt cctttttctt atccgtttgt
     6601 gatgtaacag ctaacattgc gccaccagca ggtagtttaa cttttttccc ttcccaactt
     6661 ggtgactcaa ctgctttagc agtaaaagta ctattttttt gaatgttgtc cctttgagca
     6721 atagtggtat agaccatcgc gacgttacca tcaataaagc tttgcacacc ttgttcccac
     6781 gtagtgtgta atgccgtctg gtcttttacc atcctttgat ataattcata agcagcgata
     6841 ccatctttag aagcaaaggt agctttgcca tcttttatta cacttgcccc attactttct
     6901 aaaattgctt gttgtgccca attatcagca ggttcttgaa tatacacgcc atactttcca
     6961 gttttttctt ttattatctt agaaaaagtt tgaacttctt cccaagtttt tggaccattt
     7021 tcatccaagc ccgctgcttt taatatatct tgatttaaat ataaaaccgg attactaatg
     7081 gagtaaggaa tacccacttg tttaccatct ttttcagaca gtgccatgat attgtctaaa
     7141 aaatgatctt taaaataatc agcattatcc ggatccactt cttctactaa agattgagga
     7201 ttagtatatt tgaaattttc tgcaaaataa tctttaaaag cccatcccac ttgtactacg
     7261 tccggtattt ctttggaggc aacagaagct tgtaaatttt gcatcaagcc tttatacata
     7321 tcaggattat attttgctac aaccttcact ttgtcttgag attgattgta tttatctact
     7381 aattcattca ctgtttttcc acctagggta tccgcattaa catgccaata ctctatcgtt
     7441 acacgaccct ccgatttttt tgttgtcttg gtattcccac taccacaagc tgccaaagag
     7501 atgacactta aaacagacac aacccctata acaagatttt tctttaacat aacattaact
     7561 tcctcctaat ttctttatgg ccatttttct aatcaagaaa tattatgaac taccaatgta
     7621 aatttctagt taattttctg taaataattc gaaatttgtg catttcgggg tacagggtca
     7681 ctggacatat ccatcaagct aagaataaag cccccatatg gtcatgtatc ccatctccaa
     7741 atctcttaac tcctctgttt tgattataaa atagtttatt ctgtgctcct ttcttccata
     7801 aataggccca tgcacaacgt gaggttatat tataaacacc acagcgcgca aggtactcaa
     7861 tcgtttctag ttcattgact gtccatacag aaaatgtcaa tcctagctgc tgccccactt
     7921 cacgggccaa cttagttaac catccttcat gaatattgac gatttgaacg ctttgatagg
     7981 ctaatttctg taaggtatcg atcaaatctg cttcggtaat atccccaagt tttttccctt
     8041 cgaactgatt ttcaatatta taaaatatat gctttcccca aatcgcagcc caagataatt
     8101 gtacatagcc actaagaaca atttgtggcc agatttgata ggtttgtgcc agcgaaaaaa
     8161 cggccatttc caatccctct tcttttaaat cacagttaat ggtcatggtt gccttctcat
     8221 tggccactgc ttgaaacacc tgctcaaggg ttaccgaatc tgggacaact tcttacctgt
     8281 catgggctag tattagcgta ccatctgttt gctgacgcac atctacttca aaacaaagct
     8341 ggcgatccaa acaaaagcga agaaatgccc agctattctc ctccgtattg tcactaccag
     8401 agtgtgcagt gatcatggtc attttgaccc ctcctttaat tccgtgattt tctcccacac
     8461 gtccactata ccgatgaagc gttaatttct tgttaatttc ttgtaaattc cctgcgtttt
     8521 ttcagtaaac tagttgtaaa tcttgcatgc tccttcctac gaactcatga atcgtcgatt
     8581 ttgcccttct agctagtttc accaattaag tagttctaca ttccccgctt ttaacctact
     8641 acaccttttt ttgttttcag ctattccttc ttcttgctga aagtccttga ggttctttct
     8701 tacaggatac catcaaggtg ttcaatggct tattcccatt gaatgaccga gacttactat
     8761 cagatttctg ctcgttgaac caagtctttg atacaggatt tcttccgtca tcttctcaca
     8821 aataccacct tgcgtttctc tagcgattgg tcgatgtgca ccctcccagt ggactgtcac
     8881 tacccctttt tgctatgcct agcccacaaa aaaagctgtc ctcacagaca gccagactgt
     8941 agacaaaagc tgttccaaac tcgtaactcg agcttggaac agctttttat
//
LOCUS       DACCXO010000109         8980 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.112, whole genome shotgun sequence.
ACCESSION   DACCXO010000109 DACCXO010000000
VERSION     DACCXO010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.112"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            355..1404
                     /locus_tag="IVY70_002342"
     CDS             355..1404
                     /locus_tag="IVY70_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7959455.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            1401..2282
                     /locus_tag="IVY70_002343"
     CDS             1401..2282
                     /locus_tag="IVY70_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7959456.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            2285..3055
                     /locus_tag="IVY70_002344"
     CDS             2285..3055
                     /locus_tag="IVY70_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7959457.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            3334..4188
                     /gene="aroE"
                     /locus_tag="IVY70_002345"
     CDS             3334..4188
                     /gene="aroE"
                     /locus_tag="IVY70_002345"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP7959458.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            4366..5529
                     /locus_tag="IVY70_002346"
     CDS             4366..5529
                     /locus_tag="IVY70_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP7959459.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            5646..6056
                     /locus_tag="IVY70_002347"
     CDS             5646..6056
                     /locus_tag="IVY70_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP7959460.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6230..8458
                     /gene="topB"
                     /locus_tag="IVY70_002348"
     CDS             6230..8458
                     /gene="topB"
                     /locus_tag="IVY70_002348"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7959461.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            8596..8913
                     /locus_tag="IVY70_002349"
     CDS             8596..8913
                     /locus_tag="IVY70_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959462.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
BASE COUNT         3109 a         1534 c         1934 g         2403 t
ORIGIN      
        1 aaaaatatag ttgacgaaat tcaaaaattc gattagactt aaatcaattc ttaaggaaag
       61 ggtgacctgt aatgaactta tcaaaacgta tttcagcaga aaacaaaaaa aattggcgca
      121 gctggcgtag ataagttgta ttaatggtga gactttccca tagatacaac tttttagagt
      181 gcatttctaa agcttgtatc tatgctagac atgaacattg actgccgcat agattagaat
      241 ctatgcggtt ttttgtgtaa agacaatgac cgctttggcc attgtctttt tttgtagact
      301 ggatacttgc ctgattataa taaataaaaa gattgacttt gcaggaggaa aaaaatgaaa
      361 aacaaacgat taataacagt tgtagtaatt attttagttt atttagcagg tgcatttatt
      421 tatggaaaag cctcaggcga aaaagaaagt acttcgacga accagacaaa aaaaacaaca
      481 gttgttgggg tattgcaata cgtgagccat ccggcattag atgagattta tcgaggaatc
      541 aaagatggtc tgaaagaaga aggattaaaa gaaggagaaa atcttgagat caaatttcaa
      601 aatggacaag ccgatcagag caagctagcc acaatgagtc aacagctagt acaatctgat
      661 ccagatgcct tgattggaat cgcaacacct gccgctcaat cattagcaaa cacgacttca
      721 tcgatcccag ttgtgttagg tgctgtaacg gatcctgtgg gggctggatt agtcgcttct
      781 ttagagaaac ctggtggtaa tgtaacaggt gtttctgatc agccgcctgt tacttcacaa
      841 atcaagcttg cttctgattt gttgccaaat gcaaaaaaag tcggaatctt gtatgcttct
      901 tcagaggata attcaaaata ccaagtagca caagcagaag aagctgcaaa aaaagaaggg
      961 ttagaatcag taaaatatgc ggtgccttcc actaatgaga ttgcacaaac ggttcaagtg
     1021 atgagccgac aagtcgattt tatctatgtt ccattagata ataccattgc caatgcgatg
     1081 caaacagtgg taaaagaagc aaacaaagca aatatccccg tcattccatc tgtggataca
     1141 atggtggaac aaggtggact agcaacaatc gggatcaatc aatatcaatt agggcttcaa
     1201 agcggtaaga tggcggcaga acttgcgagt ggaaaagaaa aaccggaaac gacgcctgtt
     1261 tatacatttg atcaagggga tacagtgatc aatcaatctc aagctgatcg cttaggaatc
     1321 attgttcccc aatcgatgaa ggaaaaagcc aaaatcatta cagatgactc gcagcaagaa
     1381 acatctaagg aggatgacaa atgatcgtat cagctatttc acaaggcatg ctttgggcaa
     1441 tattaggatt agggattttc atgacttatc ggatcttaga ctttcctgat atgacgacag
     1501 aagggtcctt cccattaggt gctgctgtct gtgtgacagc tattactcat ggggtagcgc
     1561 cgattgtagc tacattatta ggcgtaggtg ctggcatgat agcaggactc gttacggggt
     1621 tactttatac aaaaggaaag ataccggtta ttttagcagg gattttagtg atgtcaggat
     1681 tgaactcagt tattttgttt gtgatgcgaa caccaaatct ttctttattg aatcatccga
     1741 ttctacaaga cagcttccat cgctttgggt tacctgatta ctttgacaca gtccttcttg
     1801 gcttcatctc tttgggcatc ttgattgctg tactgctatt cttcttcaat acagatttgg
     1861 gtcaaggcta tattgcgaca ggagacaacg aaacgatggc tcggtcgtta gggataaaaa
     1921 ccgatcgtat gaaaatttta ggtttaactt tgtcgaacgg tgtcattgca ttatccggtg
     1981 cgctgattgc ccagaacgac ggatatgcag atgtaaataa agggattggc gtaatcgtca
     2041 tcggtcttgc ttccatcatc atcggtgaag tgatcttcca agaattgacg ctagcagaaa
     2101 gattgattgc tattgttgtc ggcagtatca tttatcagct attgatttta gtagtaatca
     2161 aactagggtt tgacacgact tatctgaagt tgttctccgc tatcatctta gctatttgtc
     2221 tgatgatccc gcaattgaag aaagcactga acttaaaaac gggttttgag aaggaggcat
     2281 agtaatgact gatatattga caatcaaaaa tggcagaaaa gtcgtaaaca atggaatgaa
     2341 tgaagagaaa gtactattgg atgaaattaa ccttacaatc agagaagggg acttcatcac
     2401 tgtccttggc gggaatggtg ctggaaaaag cacactgttc aatacgatcg ctggtacgct
     2461 tcaactcagt caaggatcga tctcttttaa agatcagctg atcacaaaag attcagaaga
     2521 aaaaagagca cgattccttt ctcgagtttt ccaagacccc aagatgggaa cggctcctcg
     2581 gatgacagta gcagaaaatt tgctacttgc acaaaaaaga ggtcaaaaac ggaaattccg
     2641 tttacgtaac ttgaaaaatc aaaaagaaga ttttttcaga ctatgtcaag aagtcggtaa
     2701 tggcctagag caacacttgg atactccagc gggtgaatta tctggtggtc aacgccaagc
     2761 attaagcttg ctgatggcga cgatcacagc tcctgagtta ctattattag atgaacatac
     2821 tgcagcttta gatcctaaaa cagctaaagc attaatgcga ttaacaaatc agcggatcaa
     2881 tgaacaaaaa ttgacttgtc tcatgatcac gcatcgaatg gaagacgcct tgaaatacgg
     2941 aaatcgtctg atcgtactcc aaaaagggaa aatcgttcaa gatatttctg aaaaagaaaa
     3001 gcaacatttg acaatgggcg accttttgca gttctttgaa gaagtagtgg attgatttcg
     3061 acagaaaaat tcctttcagt agaaggactt gaaaactcat tgacatcttc ctttgattca
     3121 tcctatactt aaaacaataa acaattaaac atatttgggt gttttgtagg atcacgtagt
     3181 caatgatttt aacaattaaa tagaatattc tttgctctga caagtaaacg atgagaaaag
     3241 aatttgctac tttgccacct agacgtttcg tactttttcg tatcaaacgc taagtggctt
     3301 tttttattga agtaagaaca aaggaggaat aagatgattt ctggacaaac caaattagct
     3361 gggctctttg ctgatccggc ttctcacagt ttatccccat tgatgcacaa tactgctttt
     3421 gaagcaaaca acatcgatgc ggtgtattta gctttccaag taaatccaat gaatttgaag
     3481 caagcagtcg aaagtattcg aacttttgat atgttaggtg taaatctttc gatgccgaat
     3541 aaaacagcgg ttattcctta tctggatgag gtaagccagg aagcgcaatt gatcggagca
     3601 gtgaatacga tcgtgcacga aaagggtcgt ttgattggct ataatactga tggtgcgggc
     3661 ttcatgcaat ccgtgcaaga agctgggatt tcgatcaaaa agaaaaaaat gactctactg
     3721 ggagctggag gtgcagcaaa ggcagtcact gttcaagcag cgttagacgg cgctgaggaa
     3781 atcgtagttt ataaacgaaa caaccaaaca ttcgctcaag taaaagaaac attcgaacaa
     3841 ctcagctatc gaacttcttg taagataaaa gttcgtgact ttgccgatac gcatcaatta
     3901 agaaaagact tgaaagaaag ctgtcttcta gtcaatgcaa cagatatcgg gatgggaaag
     3961 aaaatcaatc agtcaccgct cttagatata agcatgatgc atgagaatct agcggttttt
     4021 gatttgattt atgcacctag agaaacacgt ttattaaaag aagcaaaaaa agtaggagca
     4081 aaaggataca atggacttgg catgttgatc taccaaggag caatcgcgtt tgaattatgg
     4141 acaaaggaaa agatgccgat ccatgtgatt gaaaagctgt ttgattaaaa aaggataata
     4201 gataaagatc ttttattctc gcataaacgg tctcaagaaa agtaatcctt tatgtatcga
     4261 agcatttatt tcgttatttt ttattaaatt atcaaaaaat gataggcttt tgtcgattgg
     4321 ttatcgtatc ctttatttat cagtaagaaa cgaggaggaa ccacaatgag tcggatggat
     4381 cgctataagg acatccataa gaaggcgaaa cctctgaagc gggatacggg gagtattttt
     4441 gctagaagag aacgaaaacg aacgcgtatg gaaaatgagg aagatgctac ccgtatttat
     4501 gaaagagagc agcattcatt tgaacatttg aaccaatacg agcaacagat atacagagat
     4561 gcaccaagag cagaagaaca acaaaaaaat aaaaaatttt tcaatcggca aaagaagaac
     4621 aaaccgttaa aacctcgaag aaagcgatcg tggaaaaaaa tcattctatc gattttgctt
     4681 gttttgatcc tttattcagg tatctcattt tttattggta aatatgtagc tgaacatgac
     4741 acaagcttag aggcagcgac ttcagaaacc tttcatgggc aaaaagcgac gaatggtgcg
     4801 cataatattt tgatccttgg aagtgataca cgcggagaag atgccgggcg agccgatacg
     4861 atcatggtct tacagttgga tggtccttcc cataagccga aactgatttc ttttatgcgt
     4921 gacagcttcg ttacgatccc aggggtagga caaaataaga tcaattcagc ttatgcatac
     4981 ggaggagccg atctagtgcg tcaaacttta gtagaaaatt ttggaatcga ttgtcaatac
     5041 tatgcgaaag tcgatttcaa atcgtttgaa aaagtgatcg atgccttatt catgaacgga
     5101 gtgaagatcg atgcagaaaa agatctgaat ctggatggtg tagatattaa aaaagggggc
     5161 cagaagatgg atggacatgt tcttttgcag tacgcacgtt tccgaatgga tgaacaagga
     5221 gactttggcc gtgtcagacg ccaacagcaa gtaatgaacg ctattttcag ccaattgaag
     5281 aatccattga atctgatccg tgcaccttat gcagctggta aagctttagg atatacatcc
     5341 acagatgtca ctgcttggtt cttgacgaaa aacctctttt cgattgcacg aggagctggt
     5401 ggcattgatc gaataagtgt gccagtagac ggttcgtgga attatgggca aagtgattat
     5461 gctggcagta ttcttgaaat tgataatcaa atgaatcaat ctgctattac agattttttg
     5521 aataaatgat cataaaaaag atcaagaaga agctgcgcaa ggcttttgtc atgaatcaat
     5581 caaacgattc actcgtaaaa aacgctttct tatggtagat ttaaccaaga gaggagctgt
     5641 ttacattgga tatctatctg aacttcaaaa atgagtcgcg tgaagccatc gctttttatg
     5701 aaaaagtttt tcagactgaa gcatcaggta tcaccacata cggtgaaatg cctggaatag
     5761 aacaaatgaa cttgccagaa gaagcaaaaa atctaatcac gaatgcacgg ctgaacattt
     5821 atgatacatt agttatgttt gcagatacgc cagaaggaat tggtcctgaa ttagttgtgg
     5881 gaaataatgt ttctttagtc atccagacaa gaaatgaaga agaaacgaag cgcctatttg
     5941 aaagcttagc tgagggtggt aagattgaga tgccactgga tgctgttccg tgggctcagt
     6001 tgtatgggat ggtaactgat cgatttggca tcaactggca gattaaccaa gagtgacagc
     6061 taaagatgaa tattttctgg aaacatcaag cagcaaacgg atcatcgttt gtttgcttga
     6121 tgtttttgtg tctatttcga ctatacggta tctttttttt attgccagat gcgaaaatat
     6181 gttccataat agagaagtcc gcaaaaaatt aagtaacgga ggtcagaaga tgaagacggt
     6241 gatactagct gaaaaacctt cacaagcaaa agcctatgcc gaaaccttcc aacagcaaaa
     6301 gcggcatgaa gggtattatg aaatcagcga tccattgttt gctggtgaag taaccatcac
     6361 ttatggtttt ggacatttgg tagatatggt tgctccagga gcttatgaag agcgttgggc
     6421 aaaatggtct ttagaaaatt tgcctatttt cccagaaacc ttccgttacg aagttccaat
     6481 ggacaaacgt gcacaatttt cgatcgtaaa acgagaattg cagtcagccg ataccatcat
     6541 tattgcaaca gatggtgatc gagaaggtga agccatcgcg tggtcgatta ttcgacaagc
     6601 aaacgcattt tctaaggaaa agaactattt acgtttatgg atcaattctc ttgaaaaaga
     6661 agcaatctat gaaggtttta agaatctcca acctggaaag aactattttc caaaatataa
     6721 agaagcgcaa gcaaggcaga acgctgattg gctgattgga atgaatggaa gtccattata
     6781 tagcttgcta cttcaacgaa aagggattga tggcagtttt tctcttggtc gtgtacaaac
     6841 accgacactc tatatgattt atcagctgca agaagaaatc aaacacttca aaaaagaaac
     6901 gtactttgaa ggagaaggtt taattacgac aaacaaaggg cagttttctg gaaaaatcgt
     6961 accaaaaaaa gcatttaaaa cccaagaaga gttaacgaag gaaatagaaa aacttggagc
     7021 gcatttaggt aagcaaaacg gacggattct tgaagttacg aaaaaggaaa agaggttgca
     7081 tagtccacgt ttattttcgc tatctagttt gcagacaaag atgaatcagc taatgaaagc
     7141 aagtgccaaa gctacacttg atgctgctca aggattgtac gaaagcaagt ttctaagcta
     7201 tccaagaacg gattcctttt acattacaga aaatgagcat cagtatctgg ttaaacatct
     7261 gcaaaattat aaaaaatttt taggaataga agaagtacag acaactgaga tcaaagcaaa
     7321 gaaacgatat gtcgatgcga aaaaagttca agaacatcat gcaattattc cgacaaagac
     7381 gattccgact cctgcacgct ttgcgaagct ttccaaatta cagcaagcaa tctatttgca
     7441 agttttgcgc actactgtgg ctatgtttgc tgcagattat atctacgaag agacgaccgt
     7501 aatgacgggc gtacagcaat taaagctaaa aagcatcgga aagatcccat tacagcaagg
     7561 ttggcaggaa atcttacgca tcaagtcaaa aaagaaagaa gctcagactc ttcctcctat
     7621 cgtagaaaat gaagaagcag aagtagaact gaaaagtatg gagaaagaaa cacaacctcc
     7681 aaaaccctat accgaaggaa cgttgatcac agcaatgaaa actgcgggaa aaacgctgga
     7741 taatgatgaa gcgcaagaaa ttctcaaaga agtagaagga atagggacag aagctacacg
     7801 agccaatatc attgaaaatc ttaagcagaa gcattacatc gaagtcaata aaaacgaaat
     7861 aactgttacg ccaaaaggaa tcactctttg taaggcagta gccaatgaac cattactgac
     7921 gagcgcagaa atgactgcgc gctgggaagg gtacttgaga aaaattggta aacaagaggg
     7981 gacaccatct gtttttttag agaatatcaa aaaattcatc ctacatttat tggcagaagt
     8041 tcctaatcaa atcgacaagg tagacttttc agatgagatt agcgggacac gtcagataaa
     8101 agcgatggag aagaaaaacg atcaattagg aagatgtcca aaatgtaaaa agggaatagt
     8161 catgctttat cctaaaatcg ctacatgctt gaatccggaa tgcgacttca aattgtggcc
     8221 aacagtagct aaaaagaagc tgactaaaac gaatctcaga gatttgcttt caaaaggaaa
     8281 gacaagtaaa gttgtcaaag gttttactgg taaaaaaggg aaattcgatg cagttttagt
     8341 attaaaagaa gatatgacaa tcggtttttc ttttccatgg atcgaaaaca gtggaacaaa
     8401 agcagttgat ggaaacaaag aaacatcaag aaaaaataat gaaaacgaag tagagtaaat
     8461 gaaaacaatg aaatagcgga atatttattc cgaaaagtga gaatataaat accaataaaa
     8521 ctaaaaaata tcagtgaatc aagcaaaaac gttggggtag gctgatttta tatagatagc
     8581 aaggagtttt tttatttgaa aaaagtgcat tcatttttaa attatcttat gggagcaatc
     8641 acgattgtgg ctgtgtacca agcatttcta aagtatcctg attacgggac gaagttgttg
     8701 tcattgacgc acaatcaaac agcatattca ccaacgttga ttttatcgac tttgactgca
     8761 ggagctaaaa gcgctactta tttttttcat aaaacgacaa gcatgctaaa gcgaaagaaa
     8821 cagcgaaaac aattgttggt ggtggtagcg gctatgatca ttatttattt tctaccagtt
     8881 attacacaag tatttcatca atattttggt taagaatacg agagtttgtg acaaaagtca
     8941 tgtcacaaac ttttttcttt gtcatttttt cgtaagaaaa
//
LOCUS       DACCXO010000110         8816 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.113, whole genome shotgun sequence.
ACCESSION   DACCXO010000110 DACCXO010000000
VERSION     DACCXO010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.113"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(136..426)
                     /locus_tag="IVY70_002350"
     CDS             complement(136..426)
                     /locus_tag="IVY70_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959463.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(544..804)
                     /locus_tag="IVY70_002351"
     CDS             complement(544..804)
                     /locus_tag="IVY70_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959464.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(941..1354)
                     /locus_tag="IVY70_002352"
     CDS             complement(941..1354)
                     /locus_tag="IVY70_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP7959465.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1810..2295)
                     /locus_tag="IVY70_002353"
     CDS             complement(1810..2295)
                     /locus_tag="IVY70_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959466.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2434..2649)
                     /locus_tag="IVY70_002354"
     CDS             complement(2434..2649)
                     /locus_tag="IVY70_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP7959467.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2873..3673
                     /locus_tag="IVY70_002355"
     CDS             2873..3673
                     /locus_tag="IVY70_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7959468.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3692..5560
                     /locus_tag="IVY70_002356"
     CDS             3692..5560
                     /locus_tag="IVY70_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7959469.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNIFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5553..6044
                     /locus_tag="IVY70_002357"
     CDS             5553..6044
                     /locus_tag="IVY70_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP7959470.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6032..6586
                     /gene="srlA"
                     /locus_tag="IVY70_002358"
     CDS             6032..6586
                     /gene="srlA"
                     /locus_tag="IVY70_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP7959471.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6604..7599
                     /locus_tag="IVY70_002359"
     CDS             6604..7599
                     /locus_tag="IVY70_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP7959472.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
     gene            complement(7741..>8816)
                     /locus_tag="IVY70_002360"
     CDS             complement(7741..>8816)
                     /locus_tag="IVY70_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP7959473.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         3047 a         1424 c         1683 g         2662 t
ORIGIN      
        1 tgttactatg aataacataa taagaaagta aagtggattg atttgagatc tttttatact
       61 tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta
      121 atacatttta atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag
      181 agagatcata agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc
      241 ccatatttct tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat
      301 ccagcaccat ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg
      361 aagtaaaaac aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc
      421 ccccacattc aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt
      481 atgaataaca gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga
      541 agtttatttt tttctgaaat attctaacac aaatatgaaa aagatggccg aagctaataa
      601 gccgatttcg tagcaaaaca atgagatacc agagtcgtaa taataaaatc ctctaatctt
      661 atggataaaa tagatcatcg gacaagcaga taagagtaag tatttgttag gccttttgaa
      721 taagtaaaag aaagcactgg tcattatgat aaatggtatc caaaacaaaa gtttacttaa
      781 gacaatcaaa tgatcaactc ccatattttt taccaagaga gatcataaca tctttatcgt
      841 aaaaacaaaa tatttttttg ctcattttga aaatttaaac atactcgttc ttatccaaat
      901 atataaaaat cgcccctcat tcatcgagag gcgataagtt ctaatttaac tcaatttttt
      961 ttgtttcttt tggctgataa gctgttttag gtaataccaa tttcagcaca ccgtttttaa
     1021 aggttccagt gattttttct tcatcaacat taggaagata gaatgaacgt ttgtaagaga
     1081 ccgaatgtct ttctttccgt acatagttgc catcttcttt atcttctgta tgggactcat
     1141 gattggcgga gatcgtcaaa gtattatctg aatattcaac tgtcgtgtct tctttgtcac
     1201 agccaggcat atcagcagtt agtttgtatt ctttttcatt ttcgactaaa tctacttttg
     1261 gataacttga aacacctaaa aaatcattga atgctggact aaacacgtca ttgaaatcag
     1321 ggaacatgtc gcgaatgcta ggtacatttg ccatttttga tcatcctttc aataagtatt
     1381 ttttatttat ccctatttac gtaataactc tattttgaaa aaaatgcaag tataatggtt
     1441 agctttgtga atatttcatc taatacgata caaaggctat ctttctaatt tgaaaaaggg
     1501 atcattaaaa taataagaag aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca
     1561 aatatagaaa aattgaatct agaaaaccgc ccgacaataa atttcattaa ctcagttgac
     1621 cgaaaatgaa gtagatacca aaaatttatc aagagagcaa gtagagaagt gagtatattc
     1681 ataagagaaa tagctgtaac gaattattag gttaatgcaa atgcttattt atactccgta
     1741 taactttctc agaaaaaatg ataccaataa tgacttgcta gtactgtaaa tccgtataat
     1801 atcatctaat caaaatggtg ttccatttgc atctgtcatc gtaatcgatc cttcgggtga
     1861 cactcgataa aaaccaacgc ttccagatcc tccttgggaa cggatttgtt tgctggaagc
     1921 tgtgaacaaa taatctcgat caattttttg ataaaagcta ataacaatat cgtcactttg
     1981 taattcggaa cggtgggcaa taagaaatag aagtgctttg tcagagtttt ctaatggaag
     2041 agaaggtaca gtgctttctt gcatttcaga tgttgaagaa atgctaaacg actgaatact
     2101 tgattggcta tgttgcgaat ctacattacc agtagaagta ttagtaatgg aagtagtaga
     2161 agtactttta gttgaaccaa cctcagttgt tgaaatggct gctgaagatt ctttaacaga
     2221 attctttgtt ttatatccac aggcagaaaa aagtattaca gttcccaaca agcctgcgta
     2281 aatcccttta tgcatgatag ctcctcctaa aatagattat ctttattcag tatatcactt
     2341 aaacaaagaa aagcagatgg tttaaaatga aaaaaccttc ccaataaagg gaaggttcat
     2401 gaagataata acttttttaa taattatagc tttctaatat ctttcatcaa cttttctagt
     2461 aatcattggg aaaaatttca agcagtagat cgcacaaata cctacgacga tataaatgat
     2521 cttcgcaaaa atagttgttg aaccaccagc aatcatagct actaaatcaa gttcgaacaa
     2581 gcctaccaac agccagttca accctcccac aatcaataaa gttaatgcaa tagcatctaa
     2641 agttttcatt agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa
     2701 cattgcttat ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt
     2761 tgctcttcca ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt
     2821 ttagtgagct tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga
     2881 ttggttggat attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag
     2941 aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga
     3001 atgccgtata cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga
     3061 catagaagaa gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa
     3121 taatgcagga ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta
     3181 tgaattatca gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt
     3241 gatgagccaa gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct
     3301 ttcttctgaa tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc
     3361 agcgatgaac agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt
     3421 ggtaggtgtt gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga
     3481 agctctttct tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac
     3541 aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta
     3601 ttatctttca gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa
     3661 aacaagagga taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt
     3721 attgaaatta ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa
     3781 aaccagtaat caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat
     3841 tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt
     3901 tgatgagatc atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag
     3961 aatcgcttat ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt
     4021 gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga
     4081 gatgatcaat gattttgatg taaagattac aggcacacca aataaaggac taagaataat
     4141 aggagatgag ctagaattac gactactttt attttatttt gtttctccct acttttcaga
     4201 gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac
     4261 gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag
     4321 aatctctagt aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata
     4381 tatccaagaa ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag
     4441 ccaatatgaa aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat
     4501 gataaggaaa gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg
     4561 acgattagat ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct
     4621 ctctttattg ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga
     4681 gatagaaaaa aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga
     4741 agctatcttg caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga
     4801 gttagcattt cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac
     4861 gacaggaaaa ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga
     4921 tatacaaata acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt
     4981 cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt
     5041 gactaatatt tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg
     5101 attagccggg tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta
     5161 tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga ggctattatg tggatgaaga
     5221 atttgaaaaa aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt
     5281 tgcttttcct cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat
     5341 aactgatcaa ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc
     5401 tgaagaaatg acggctgaag ttgagaatga attatttgag ctatacgata tcctatttga
     5461 actcataaaa atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt
     5521 aaaactaatc aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt
     5581 tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca
     5641 atcaaacata tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga
     5701 aaatccgttc aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag
     5761 tcagaaaaat gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg
     5821 gtgaagatat ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga
     5881 cacaagcagc tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa
     5941 aaagtgaaca accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa
     6001 gtacgatcaa agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg
     6061 atttatgagg ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt
     6121 accagttgtc ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga
     6181 ccgagtaacg aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt
     6241 accgttctta tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct
     6301 tccagaatac tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa
     6361 tggtgtattc ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg
     6421 gatcgaaacc ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact
     6481 gctgatgaac tttattggtg gttggataac tgattttact actggatttg tatgcagaca
     6541 gcaaggaatc gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt
     6601 taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt
     6661 attaggccga caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca
     6721 atgatcgtag ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca
     6781 tcaatacctg atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt
     6841 ggaatttatc ctaaaaaagg gataccaacc atcaatatcg tttccacagg aaaaagtggc
     6901 ccattagcac aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata
     6961 cagccactaa tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa
     7021 aagtacaaat atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg
     7081 atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga
     7141 gaagcagtag atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg
     7201 attggtgtga tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta
     7261 gcaggaaatg cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct
     7321 cctttgcttg gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag
     7381 attgggaaag gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact
     7441 caaaatgcat gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca
     7501 gtagaagtag gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt
     7561 atcattgctt gggtagcaag ttttggatta tatagctgag tttagtctaa tggtgataag
     7621 tcaagatgaa atttaagatt ttctttattg agtaatatgt tttgaagcgc acgaaactag
     7681 acccgatcaa aatcaaattt ttttcgggtt gattcaagga tttgcgtcaa taacggattg
     7741 ttatttctct atcacactcc ttggtggcgt atagagttgg tgtttacgta gtagcgtatc
     7801 caccagacgc gtaaattttc ttgcggttag gacgagtgca cgtttgtgtt gatgctttgg
     7861 aacctcctta tatttacttt gataaaaagc tttgtattca ggaagataat tttttacaga
     7921 gttggcagct tcaactaagt agtatctaaa atatcgattt ccttgttttg tcagaggtgt
     7981 attttgcgac tgatagtttc cagattggtt cactttccaa cttaatccag cgtattttgc
     8041 caattttgtc tgatcttcaa atctttcgat ttgaccaatt tcagcgatta aaccggcagc
     8101 atagacttta ccaacgcctg gaatactggt taagcattga tattctggaa tgacaacaat
     8161 tacttgttcg atggctttat ctaattcttt gatatttttt tcaagtgctc gtatttctcg
     8221 tacgagaaca cttaaaacaa cattaattga ttcttgtgca agagcaccta ggcggtaact
     8281 catggtaatt gcacgttgaa ttgctttagc gattttttct ggttgcttga aacggccttt
     8341 tcctttttct tgaagtaaat cacagaaagc ttctaaagga agctcagcaa gttcatccaa
     8401 tgtgaaatct tccgtcatga gtgaaatgag ggttgcacta aaaagactcg tcgattcgtc
     8461 tcgcatttct cgagcaagcg tattacattt gtacgttaga ttttcaagaa aatgttgctt
     8521 tgtacgaatc aattgcttaa taagttgata gcgagtacgt gttaaacgct gtaaggccat
     8581 gtatttctct tctttgatag gggaagttgt gaatcgttga atacgcaaga aatcagcgat
     8641 tcttaatgca tcgtttcgat cagttttatt ctcatcaaac atgcgactaa attgtttcac
     8701 ccgaaaagga ttttcaatcg ttacaagtgt gttgagcttc tttagtttct catcttcatg
     8761 aaaaaacatg gaaggatgga agctgtacat ggacgttgac tccatgccaa tcacaa
//
LOCUS       DACCXO010000111         8800 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.114, whole genome shotgun sequence.
ACCESSION   DACCXO010000111 DACCXO010000000
VERSION     DACCXO010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.114"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            9..542
                     /locus_tag="IVY70_002361"
     CDS             9..542
                     /locus_tag="IVY70_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959474.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(668..1345)
                     /gene="nth"
                     /locus_tag="IVY70_002362"
     CDS             complement(668..1345)
                     /gene="nth"
                     /locus_tag="IVY70_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP7959475.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1352..2050)
                     /locus_tag="IVY70_002363"
     CDS             complement(1352..2050)
                     /locus_tag="IVY70_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP7959476.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2318..3502
                     /locus_tag="IVY70_002364"
     CDS             2318..3502
                     /locus_tag="IVY70_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP7959477.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3508..3951)
                     /locus_tag="IVY70_002365"
     CDS             complement(3508..3951)
                     /locus_tag="IVY70_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7959478.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4177..4464)
                     /locus_tag="IVY70_002366"
     CDS             complement(4177..4464)
                     /locus_tag="IVY70_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959479.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4635..5987)
                     /locus_tag="IVY70_002367"
     CDS             complement(4635..5987)
                     /locus_tag="IVY70_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7959480.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSDPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQEALGEIETAQ
                     "
     gene            complement(6259..6705)
                     /locus_tag="IVY70_002368"
     CDS             complement(6259..6705)
                     /locus_tag="IVY70_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP7959481.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6941..7867)
                     /gene="cysK"
                     /locus_tag="IVY70_002369"
     CDS             complement(6941..7867)
                     /gene="cysK"
                     /locus_tag="IVY70_002369"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP7959482.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(8007..8800)
                     /locus_tag="IVY70_002370"
                     /pseudo
     CDS             complement(8007..8800)
                     /locus_tag="IVY70_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
BASE COUNT         2402 a         1784 c         1450 g         3164 t
ORIGIN      
        1 tacaatatat ggttttgaat aattttttga aagaaggctt cattttgagt tatctgatag
       61 tatgcttaat tatcataatt tgtctgcacc atcttttttt atcgaaaaca atacctcgtt
      121 attccaagaa taaaaaagct gaaaaatttt gtttattatt aaataaattt actttaattg
      181 ctcctatcct tgcatttatt attttttctg tacttttaag cactactctt aaaggaaaat
      241 ttatggaaag aagctctcat gcaatgattc tcactttttt atggttgttg ttcaccagaa
      301 tctatatttt tctgatgagc ctgaaaccgc ccaaatccat tagtttatgt ttagttataa
      361 atggtatttt tttattatca cttatcattt ttattacacc tttagataga tacgtcactt
      421 atttatataa tcctttggaa tactggactt attttatagg tatattggaa ggcattattt
      481 tctacatcgg gtattttccc aataaaaata acaactttta tttttatcgc aataaattgt
      541 aataagttac catcctagct actgagtatg tacatttact ttttcatgca aaaaagataa
      601 acgaaacagc ttttctccat aataacaaga taattcatac atttatttgc aattaaagaa
      661 accacgtcta caggcgtggt ttctttttta gcgccttttc ttttagacgc atccgatttt
      721 tcccatcttg acatattgat agcaaaggac atacctcgca cttgggattt cttgccgtgc
      781 aatgatatct accaaaaaaa atcagtgtat gatgtgtctt tacccataat tcttgtggta
      841 ctttccgcat cagtgtttct tctacttcca tgaccgtagc atctagtttg cagatcctca
      901 ggcgttttga gacacgttcc acgtgggtat ctactgcgat tgctggtatc ccgaatgcat
      961 cccccaatac gacatttgct gtctttctgc ctacaccagg aagagacatc aattcttctc
     1021 ttgacgttgg cacttgccca tcgaaacgtt caatcagctg ctgtgcacac gctttgatgt
     1081 tttttgcttt atttcgatac aaaccaatcg tttttatttt aagaatgatt tcatcgattg
     1141 aagcttctgc taacgcatcg ggtgtcggaa aagaggcaaa caaatctggc gttgctttat
     1201 tcactgatac atctgtcgcc tgggcactta agatcactgc aatcaataac tcaaatggat
     1261 tattgtgctt gagttctcca tgtgcttcgg gaaacattcc atacatcgtt tctagagctt
     1321 ccattgtttt ttgtttgctt aacatattcc gctactcctc caaccaattt ttcagtgaga
     1381 ctttcggcaa ttcttcttgt tgagctgact gttctacttc tctttgaaga agctgctgtt
     1441 ttcttcgttt ttggtcttct tccacttgtt gttttgtacg tagatttttt ctttcccacg
     1501 atagcaaaat gcggtcaacg taattaaaac tataagcctg atttaatacg gcttcccgca
     1561 gtgctagctt caaaatttcc ggctgataat ggtcttcttc tagccattgt ccgatccgct
     1621 ggaactcgat cgaagaaagc gggcgtccaa attcttgttc aaataattga tacactgact
     1681 ggatttcttt ttcttgtgtt ttttcttcgt attttttttc ttgtgttttt aaatactcac
     1741 ctaatagatc atagatgggg taaagattat atcgatctgc ttttttaccg ttgacaatcg
     1801 tcgtttctat cttgataaat ccatttgtta cgagacgatc cagtatttga aaaatctgat
     1861 cttgtttgat tcccatatcc atagcaatca tctgcaaatc agggaaagta tctccttcaa
     1921 gctgtgccat atgcaactgt agaacaaata aaaattcttc agattgtaga ccgatcctat
     1981 gataattttt aagtaaaaga ttagatatga ctgtttgtcc agcgcttaag taattttcta
     2041 aattcaacat gatttctcct ccgctcaatc acagtatacg taaaaaaaac atgggaagca
     2101 agaacaggtt tcttttctat ttacttcact gatcggtcag atttacaatg tcgtctactt
     2161 gatttttgtt tgttcatgtt tttgatgcgt atcttcactt ttcggaataa ataaattaag
     2221 tagatttcgc tgttccttag aatgttgaca aggaaacaaa attactatac actatttttg
     2281 tttccaagtt aacattattg cataaaggag tttttacatg tacagtttca caaataaaca
     2341 ttctaaagtt tttggatacg tctctgtctt attcactgga ttaggattta ctatcgtcag
     2401 tcctgtactt ccgtttttaa cgctgcctta ttctcactcc atacatcagc aagctttcta
     2461 tatcactttg ctcatgtctg tttatgctct agctgccttc ttatctgccc ccattctcgg
     2521 tagcttaagt gatcattttg ggcgtcatcc gatccttatt atcagtttat taggatcaag
     2581 tatcggatat ttgctttttg gtttaggaaa ttcgatatgg atgttgttcc ttggtcgtat
     2641 cattgaagga ttgacggcag gagaaatctc caccttatat gcttactttg ccgacatcac
     2701 ggaacctaat gaaagaacca aagtttttgg atggatgggt gctttagtcg gtataggaac
     2761 aacgctgggt cctattatag ggggtctatt agctgagttg gggaattctg ttcctatatt
     2821 tattggagca ctctttactt tcttaaatgc cgtttatggc tacacattta tgctagaaag
     2881 ccttccaata aaaaaacgtt cagtagattt aagtttctcc catgtaagac cttttcacca
     2941 attacaacaa ttattcaaga tttcatccgt tattccttta ttgacagcag gttttgctgt
     3001 ttggttggca gctggctctc ttcaatctat cttttctcaa ttttcgattg acgcctttca
     3061 gtggaaggct gggttggttg gtttaagctt ctctttgatt ggaatcttgg atactgtctc
     3121 gcaattgttc attatgcctc gattgctcaa aaaattttca gaacagcaga tcacaagaat
     3181 agggatgttc agtgaaatcc tcgcctatct attcatcaca ttatctggaa ttcttctact
     3241 gccgattttg tttttatttg ggattatctg ttatggtttc ggtgattcta tttttactcc
     3301 tgtattcaat ggccaactat ccaattctgt ctcagataac cagcaaggtc ttgtaatggg
     3361 cggtacccaa agtatacaat ctctatcgcg cgtcatcggc ccgctaatcg caggacagct
     3421 ttatgctgca gcaccatttc ttccgacggt attcggattt ttgcttatga tttttgccta
     3481 ttatcactac aataaaaaat agtagcttca tttgctactt cgtataaatt ctctgtatcg
     3541 accgataaaa cgaaatactg tgtcaaattc ttcttttgac atattttcaa atacagtagc
     3601 atctcgctgt acccattttt gatggagttg ctcgtgtcta ttccttattt tttgtccgat
     3661 tggcgtcaat tgaaaataga tttctttttt attctcttcg ctttgataag tgtttatgat
     3721 ccctctagct atcagttttt ttgttagctt actgatggct cctctagtca tgtaactagc
     3781 attcgctaat tttgtcacgt taggatcagg gattttcccg atatgttcaa tcacttctag
     3841 ttcatttaga gtaaatcctg caaattcttt ttccatttct ggtttattac gatgaataat
     3901 ctcattagct aaatccagat attcttctat tagcagttgt cttttatcca tttctacacc
     3961 ttctttttct ttcattatat tgtttttttg tttcctattc aacaaagata aaaatatttt
     4021 gtaggaaaac ccaatctgaa accaagaaaa aaaggggttg acaaagcttg tcataacccc
     4081 tttttctgat taaaagatag taaagagaca gtcatcggta attgtgatga ttttttgtca
     4141 tcacttaagg atgagcggcc ttttcaaaaa ctgtctttaa agatcatcat cagtttctag
     4201 cacatatggc tgaccttcat atgtgtactc tgtattcaat gtgccgtcat tttcatctga
     4261 gtagatgcgt ctactgaaag gaaaacgatg agaaacatta tctttggcgt catttgctgg
     4321 catcgtgtaa tattcctctt gcgtttcatt caactgagag agtacttgat ctgccaactc
     4381 atcaatattg atcgttaatt tcttcgaagc acttttataa tcttctaaaa atgcatcttg
     4441 ttggacaaaa tattctttat tcatcttctt tctcaccttt cttttcaaaa tcaaataaag
     4501 ctgcttgttc ttttatgtaa cagctttatc tttgctgctt attgtgacaa aaaattcgaa
     4561 gaaagacaac taattgtact tttcacaaaa aaagacagag ggtcgtgaca aaattgtgtc
     4621 acaacccttt ttctttattg tgcagtttcg atttcgccta gcgcttcttg tgcaagtagg
     4681 ttcaagtaat tccatgggcg gtcaaaatgt ggttggaaga agaaatctga aagtgcgaga
     4741 tcttctacag tcattttatt ttgaacagct aaagacatgg tatttgctga ttgtgtaatg
     4801 tcgtatttgg acatgaactg agcacctacg atacgattcg ttcctttttc atagactagc
     4861 tccatcatta ctttttcagt agttggcatg aactctggac gataattatc ttcaaataca
     4921 gtcgcttgga cttctagatc gttcaaagtt gcgctttctt tggtcacacc tgtcgaaccg
     4981 atcgtccagc caaataggta aagtcctgaa gttccttgag taccacgata cgccatttta
     5041 tgatctgtca agttatttcc cacaagtaaa ccttgacgaa cggcatttgt tgccaaagga
     5101 atatagtttt gcgtgccact tggattgtag ttgactactg cagagtctcc agctgcaaaa
     5161 atgtctggat cacttgtctg catgtattca ttcactttga ttgccccgtt tggcagtgta
     5221 tctacttttc cttcgatcag ttttgtattt ggacggaagc ccacacataa aatgaccata
     5281 tctgtatcaa atgtatctgt tggtgtagct acttgtttca cttttccttc ttcatctgtg
     5341 ataaattcag taacattttc gcctagcgca agagtaacac cgcggtctgt caattctttt
     5401 tctaagatat ctgtgaatgg tttatctaga tatttattca agatgcgatc taatccatca
     5461 atcaatgtca cttgttttcc agattcgaca aaagcttcta ctaattcaat accgatatat
     5521 ccaccaccaa caattactac acgttttgct tctttcgctt gtgcaataat ttcatttgct
     5581 tgattgtagt ttttgcatag gagtacattt ttcgcatcga ttccactaat cggtgggata
     5641 ataggccatg aaccagttgt catcactaat ttatcataag aaaccgtttc tgtttctcct
     5701 gttttcagat cttttgccgt tactgtttta ttttctgtat cgacgtctgt tacatcgtgt
     5761 tccattttaa ctgttgctcc taatgaagat aattcttcag gatttgagta gaacaagcct
     5821 gctgggtcct ttactactcc gccgacataa agtgcgatac cgcaagataa gaaagaaata
     5881 ttgtcatttc tttcatatac tgtcacttct gcttctggat gattttttaa aatacttttt
     5941 acagctgcag tacctgcatg agtacagcca attactacta ctttcattta tggtccctcc
     6001 gattaacatt ttgtccttcg acaaaactac tataacagaa ataaaacgga tacaacagtt
     6061 taatgcttgt gaattcacaa aggtttgttt gtagtttcat tccttttcaa gcataagaac
     6121 ccaatatcta caagattctt attgtgataa tattcacttt gatatctaag attgtgaata
     6181 aaagacaaaa aaataaaaaa gctagcgttt ttttacacag ctagcttttt ttaggtataa
     6241 cacttataga aactgttttt acatttgcgc cttttttgct gactctttac aatccggaca
     6301 gagtccatac aattccaacc gatgttcatt aatctcaaaa tcagtcagtc tgctcgcagc
     6361 catttctata tcttcaagcc ctgggtaatg aaaatcaaca atcttaccgc agttttcaca
     6421 aattacatga taatgacgct ttgaactgaa atcaaatcgg ctagaagcat cgccgtactt
     6481 catttcttga acaaatccaa tcgaagtaaa taagcgaagg ttattgtaaa cagtcgcaac
     6541 actcatattt ggaaaccgat gttcaagtgc tttataaatc tcatcagccg ttggatggga
     6601 atgatgttca atcaaatatt ctaaaaccgc gtaccgttga ggtgtgattc gaatgttctc
     6661 tttttttaac ttttcgataa cctcttcgac taacttattt tccatgtaaa caccccacgt
     6721 tgttattctc gtcttatcat actattttta cattttaaaa tccacttaac atgaaaagaa
     6781 aattcaattt tattgtattt taatgaaaaa agacgtaaaa cagtcgcgtt cactatttta
     6841 cgtctctatt caaataattt ttctcatcat aaatttttgt gtgttctttt catctttaaa
     6901 agttttttac ataaacctat ctggagaaaa accaagtgcc ttatatctct tggtaaaggg
     6961 ctgtagataa ataacgttca ccattgtcgg gaaccacagc taataccttc ttgccttttc
     7021 ctaattcttt tgcgactttt agcgcggcag caattgcagc gccagatgag atgcctacta
     7081 aaatcccttc ttttcgccca acttctctag ctgtttccat cgcttcgtct ccactaatag
     7141 aaaggacttt atcatacaca gaagtatcca gtgttttagg aacaaaacct gtaccgattc
     7201 cttggatttt gtgaggtccc gcttctttcc cttctaaaat agcagattct gctggttcta
     7261 ccccgattaa ttcgattttc ggatacacac gttttaattc tcggccagcg ccagtgatcg
     7321 ttcctcctgt accgatacct gcaacaaacg cgtccaagcc atttactcca aatgcttgat
     7381 gtatctcagg accagtcgtc ttctcatgca caagaggatt tgcttcattt tcaaattgta
     7441 atggtaaaaa gtagccgttt tcttttgcca aacgttctgc ttctctgatt gatccagaaa
     7501 taccatccgc accaggagtc aaaatcaact ctgcgccgta ccctttcatc aataaacgtc
     7561 tttcgatact catcgtctca ggcatcacga tgattacttt atatcctttt gcaactccta
     7621 ccatcgacaa tccgattcct gtatttccag aagtaggttc aatgatagta tcacctggtt
     7681 tcagtagacc atcgtgttct gctttttcta tcatacttaa agcaatacga tcttttacac
     7741 ttcctcctgg attaaaaaac tcaagcttta caaaaacatc tgctgaatct tctggtacga
     7801 ttttatttaa tttgacgatt ggcgttttgc caatcaattc ggtgactgag ttatagatag
     7861 ctgtcatctt caattcctcc ttgtctttgc tcttcttact tatcataata caatctgttc
     7921 atttatggcg agtgattcgt ctttgactag caaaaaaaag acaaagatat accatatatg
     7981 gtagtttctt tgccttttta tctaaatcaa acgtttagtt cttgaccaac taataaagaa
     8041 taactggaca tagacatgcc atttttcgcc attagatcat caacagacat gccaaatttt
     8101 tgtgcaatat cccatagcgt atcacctgac acgactgtat atgtctgacc attatttgta
     8161 tttacatctg ttgttgaaga tgatgacgtt gaagtcgtcg tagtagtcgt tgttgttgta
     8221 cttgtggtag ccgaacctgt tgatggcgta tcaaaacggg ttaaattata agtattaatc
     8281 aatgagttca atttggcccc ataatttgga tcggtagcat aacgacctgt caagtacgct
     8341 gttgcatcat aaaaacttgt tgtattgctt ttccacacgc cagcataatg ataatcgccc
     8401 gtggaaaaac tggttgaacg aagaacattc gcatggtctt ggaaggattc ccaataagaa
     8461 gaatattttc ggaatggttc attcatcgtt acccattgtc cgttacatat tctttggttg
     8521 ccatataaac gacctgtccg ttatagcttc ctttcacacc gaatagatta taatttggcg
     8581 cactagctaa tccggaagtg ccatatgcac tttccaacaa cgcttgagcg atcatgactg
     8641 aagcatacaa gtcattactt gcagcaactt ctgacgcaga ccagccgatt tgatcgataa
     8701 acgcttgagg atcagcaact gtttgagatc cttgttcatc tgcatgaaca tgttgcggta
     8761 ccgctgtaac catcatcggc ccaactaaaa gactcgttcc
//
LOCUS       DACCXO010000112         8764 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.115, whole genome shotgun sequence.
ACCESSION   DACCXO010000112 DACCXO010000000
VERSION     DACCXO010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.115"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(258..2639)
                     /locus_tag="IVY70_002371"
     CDS             complement(258..2639)
                     /locus_tag="IVY70_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP7959483.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2629..3276)
                     /gene="recU"
                     /locus_tag="IVY70_002372"
     CDS             complement(2629..3276)
                     /gene="recU"
                     /locus_tag="IVY70_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP7959484.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3340..3879
                     /locus_tag="IVY70_002373"
     CDS             3340..3879
                     /locus_tag="IVY70_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP7959485.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3969..4388
                     /gene="gpsB"
                     /locus_tag="IVY70_002374"
     CDS             3969..4388
                     /gene="gpsB"
                     /locus_tag="IVY70_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP7959486.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4410..4777
                     /gene="rnpB"
                     /locus_tag="IVY70_002375"
     ncRNA           4410..4777
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IVY70_002375"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4986..6152
                     /locus_tag="IVY70_002376"
     CDS             4986..6152
                     /locus_tag="IVY70_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP7959487.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6198..7694
                     /locus_tag="IVY70_002377"
     CDS             6198..7694
                     /locus_tag="IVY70_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP7959488.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7775..8458
                     /locus_tag="IVY70_002378"
     CDS             7775..8458
                     /locus_tag="IVY70_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7959489.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2765 a         1584 c         1858 g         2557 t
ORIGIN      
        1 aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta taaacaagta
       61 aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt taagctaaat
      121 gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat acagcttggt
      181 acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc acgcttgtag
      241 acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg
      301 tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat
      361 gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc
      421 tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta
      481 gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag
      541 ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct
      601 ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt
      661 gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac
      721 gattctgaag tgacaggtgt cattttttta ttgtaacctg tccacacgga aatcgaataa
      781 tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt
      841 gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg
      901 atttgcgcat tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata
      961 taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg
     1021 tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa
     1081 gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct
     1141 tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt
     1201 tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca
     1261 ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag
     1321 tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca
     1381 gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa
     1441 ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg
     1501 tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca
     1561 acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt
     1621 ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc
     1681 ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta
     1741 gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct
     1801 tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct
     1861 cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc
     1921 atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac
     1981 atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag
     2041 taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct
     2101 tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc
     2161 aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca
     2221 gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc
     2281 gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt
     2341 tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta
     2401 gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct
     2461 acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat
     2521 aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca
     2581 ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt
     2641 cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg
     2701 aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg
     2761 tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt
     2821 caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg
     2881 ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga
     2941 tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt
     3001 atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt
     3061 aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc
     3121 gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt
     3181 gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt
     3241 ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca
     3301 ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc
     3361 tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc
     3421 caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa
     3481 tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa
     3541 ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt
     3601 aataactgga atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat
     3661 gtcgaagctg ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt
     3721 tttatacttg agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa
     3781 acgaagtatt tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg
     3841 ctgatcagta tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat
     3901 caactttttt cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag
     3961 gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa
     4021 caaaaatgcg cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag
     4081 attatgaaac ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg
     4141 caaaagtagc tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag
     4201 aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg
     4261 aacgtgaagt atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac
     4321 ctaatccaaa taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc
     4381 aattttaatt tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca
     4441 ggctgaggaa agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa
     4501 accataagct aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat
     4561 gcttaagtat ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga
     4621 acgcggtaaa cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa
     4681 gaagaacagg acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct
     4741 ccctggaaaa gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag
     4801 gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa
     4861 tacttcagat gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat
     4921 ttgagtaaaa gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat
     4981 tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag
     5041 cactggttgg taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg
     5101 cacgttttga aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag
     5161 atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa
     5221 acgtaaaagc attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg
     5281 gaaaatcgat caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag
     5341 caatcgttga acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa
     5401 ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg
     5461 atacgacagg accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat
     5521 tgaaagaaaa catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat
     5581 tttatgatcc tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata
     5641 atatcgcacc aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag
     5701 aaatcatgga aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag
     5761 atattactgg ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag
     5821 aaatcggatt aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg
     5881 ataaagaata cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag
     5941 aaactgttcg gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga
     6001 gtaaatatat cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca
     6061 aaaaaagaaa attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc
     6121 gtccgcctag aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat
     6181 gtttggagga atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc
     6241 ttctacaaca agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag
     6301 ctagtgcata ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa
     6361 tcggtccaaa aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg
     6421 aagttggaac tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac
     6481 cggaagatct tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa
     6541 aagctagaga aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat
     6601 taacaaaaca gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc
     6661 tgaatcaata cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta
     6721 aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag
     6781 actttttaaa tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg
     6841 aacaacttgg ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga
     6901 ctggaattaa tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa
     6961 tggctgtttt tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg
     7021 ataaatatgc gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat
     7081 cgttgtttaa cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt
     7141 tctttcaaga atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt
     7201 cattgaacca tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc
     7261 tccatatcat cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg
     7321 ctgaactacc tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa
     7381 atgatgtgga aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc
     7441 cctcttatgc attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa
     7501 tagatgtgga aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag
     7561 agcatatcca tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac
     7621 atgaaccgct taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat
     7681 atcaggtaaa ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt
     7741 tttcagctat ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga
     7801 ttatagtatg gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag
     7861 agttgtcaag ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac
     7921 gcttgcagca gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga
     7981 ggcaaagcaa cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc
     8041 gatgattttt tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga
     8101 actatctctg gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat
     8161 cggcattttg cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga
     8221 actgtatccg caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa
     8281 tcatttttat gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga
     8341 agaagccaaa cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg
     8401 gtctattgac gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg
     8461 gaggttgtga cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac
     8521 ttctggaaca ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga
     8581 acaatttcaa aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg
     8641 gcaggagaac accgtttatt cggaagaata ggactgtgac aagacttttg tcacagtcct
     8701 tttttcgaat tcaacaagta aaacttttaa gaaatattca gagagtaaaa ttaaaaattg
     8761 ctga
//
LOCUS       DACCXO010000113         8735 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.116, whole genome shotgun sequence.
ACCESSION   DACCXO010000113 DACCXO010000000
VERSION     DACCXO010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.116"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(93..539)
                     /locus_tag="IVY70_002379"
     CDS             complement(93..539)
                     /locus_tag="IVY70_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP7959490.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(566..742)
                     /locus_tag="IVY70_002380"
     CDS             complement(566..742)
                     /locus_tag="IVY70_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP7959491.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(904..1332)
                     /locus_tag="IVY70_002381"
     CDS             complement(904..1332)
                     /locus_tag="IVY70_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP7959492.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            1424..2284
                     /locus_tag="IVY70_002382"
     CDS             1424..2284
                     /locus_tag="IVY70_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP7959493.1"
                     /translation="MTSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            complement(2376..2669)
                     /locus_tag="IVY70_002383"
     CDS             complement(2376..2669)
                     /locus_tag="IVY70_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP7959494.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(2741..4468)
                     /locus_tag="IVY70_002384"
     CDS             complement(2741..4468)
                     /locus_tag="IVY70_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP7959495.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            complement(4682..5608)
                     /gene="trxB"
                     /locus_tag="IVY70_002385"
     CDS             complement(4682..5608)
                     /gene="trxB"
                     /locus_tag="IVY70_002385"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP7959496.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(5753..7186)
                     /gene="ascB"
                     /locus_tag="IVY70_002386"
     CDS             complement(5753..7186)
                     /gene="ascB"
                     /locus_tag="IVY70_002386"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP7959497.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(7186..8589)
                     /locus_tag="IVY70_002387"
     CDS             complement(7186..8589)
                     /locus_tag="IVY70_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP7959498.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
BASE COUNT         2377 a         1950 c         1475 g         2933 t
ORIGIN      
        1 aataagattc tttcttctag ctgtttttta gttctttttt tacgaacaca aaaaactgcg
       61 gataatcact gtccgcagtt taaatgataa aattaagatt gtaatagttc tttcacaatc
      121 gcattcactt ggtttccatc tgcttttcct ttgactttag gcataattgc acccataact
      181 ttgccaaatt ctttcattga tgaagcacca gtttgagtga ttgcttcttg aacaagttgt
      241 cgaatttctt cgtcggaaag ttgttccggc atatattttt cgactattac aatttcactt
      301 tttacttttt cagctaaatc gtcacgtcct gcttcttcaa actcatgcaa agaatcacgt
      361 cgctgtttca tttcacgaga caacaccgtt agttcttctt cttctgtcaa gtcgtgacct
      421 tctttaatct gctcgttttg tatagatgac ttgatcattc ggatgacttg taaagattcc
      481 ttatctttcg ctttcatcgc tgctttcatg tcatcgttta atctacttag tagtgacaca
      541 ctaccaactc caattcatca cagaactaga attttttgcg ttttctagct gcttcagatt
      601 ttttcttgcg tcttacactt ggtttttcgt agaattcacg tttacgagat tcttgtaaag
      661 tacccgcttt tgacacggaa cgtttgaagc gacgaagagc atcgtcaagt gattcgtttt
      721 tgcgaacgac tgtttttgac atgttaattc cctccctccg agcttaaaaa gtaaccgtaa
      781 ttgttattga aatatatctc aaaaaacaag actgtcctgt aaatattata tctaatgctg
      841 ttttgaccgt caaggaaatc ttgctttttt ttaaaattat caaatgttat tttatttaaa
      901 tccttattga caattttcac atcttccgaa aatctcgaat ctatgccctt cgatcgtgca
      961 tccacctagc tgttcttcga aataattcat cggacacata tggatttctt tcgttgttcc
     1021 gcacactgta cagataaaat gatggtgatg gccgattttc ccctgacagc aatgaaaacg
     1081 aaacttcatt tctccattta aatcggtttc ttccagtaaa tcgatctctg caaaatcatg
     1141 gagattccgg taaatcgtat cataacttac ccctttgaat tcctgattca tatagtcaaa
     1201 aacttcttta gcagatacgt agcggttacg cttcactaaa taagttaaca atgcttctct
     1261 ttttttagta tatttaaaac cgttattttt caatgtttct aatgattgtt cgatcaatgt
     1321 ctgttttttc atgacttatc cctccaaatc aaaatgatta cgaagtacgc attctagagt
     1381 atggtataat aaacataaaa aaaatgcaag tgaggtttta tccatgactt ccgattctac
     1441 agaaatcgtt actttttttg tcatctccga ctctgctgga gaaactgcga caaaactagc
     1501 gcaagctact atggctcagt atcctaccgt tgagttcaac ctattccgca gaacgttcgt
     1561 gacagatgaa aaaacattaa ggcaggcatt gcaagatgca ttagaagaaa aagcacttgt
     1621 cctgcataca ttaatcaatc aagaactgat cgagataaca cgttcttttt gtgaagaaca
     1681 tcaattattt agttttgatg tgatgacgcc accgatatca gaaattgaac gtttaactgg
     1741 tatcaagcca attcggcaac ctggtgccct ccatttattg aatgagaact atttcaaacg
     1801 gatcaaagca atggaatttg ctgtaaaata cgatgatggg aaagatcctc gtggtttttt
     1861 agaagcagat gtcgttttgc taggcgtatc ccgaacttcc aaaacgcctt tgagcttgtt
     1921 tttagctaac aaaaatttaa aagttgccaa tttacctttg atcccacaag cacatattcc
     1981 caaacagctt tgggagatcg atcctaaaaa aatcgtggga ctaacaaata acccagatat
     2041 tttaaataat atccgtaaag aacggatgcg ctcttacgga ttaaaccctg atactgctta
     2101 ttccgatatc gaaaaaatta gagctgaact cgattttgcg aatgatcttt atgaaaaact
     2161 aggatgtgta gtgatcaatg tagcatcttt atctattgaa gaaacggctt cgcttatttt
     2221 gaatgcttta gacctagaag atcatagtta ttatggaact gaaacaacag aaaaaagcga
     2281 ttgatcattt ttgtcttttt cttaaaaaac actggagtgt gacaacgaaa tttcttcgtt
     2341 gtcacactcc agtgtttatc tatttgcacc tatcgttatt gatgttccac atgtttgagt
     2401 gtctggttca aaatatccag tacatgagaa tcatcaagac tatacaaaat cgcttttccc
     2461 tcgcgatgag acttgacgac atgattttct cttaataaac gtagttgatg cgatacagca
     2521 gactgctcca tttccactgc ttgggtgatt gctgtcacat tttgttcccc gtctttcaga
     2581 tatagcagaa ttttaagacg agtgggatca ctcaacattt tgaataattg actgactttc
     2641 tctacttttg atttttgcgt gtctggcaat ccattcacct ccaacttgaa aaaagactga
     2701 cttctaaagt ttaccacttt ttccatcagt ctcaagtttt ttattcttct gtcatctttt
     2761 tgataatcgc ttcataagag gctacttttt cggctgcgtc ttctgtagaa cttcctttta
     2821 caccaatata aaacttgatt ttcggctcag ttcctgaagg gcggatagca atccagccat
     2881 tttcttccaa tgtatacttc aatacgtcgg atggcggtgt tgttagtttt tctacgtttc
     2941 cttcacggtc tgttctcgtc aattctttaa agtcttctgt ttgaataact tttgctccgg
     3001 caaattctgt cggtgcttct ttacggaatt tttccatcaa agcagagatt ttcgcagaac
     3061 cttcttgtcc gctcattgtc acagaaatcg ttttttctgc gaaatagcca taggtttcaa
     3121 aaatcgtttg caagccgtca tacaacgttt tgccttgttt tttatagtaa gctgctactt
     3181 ctgctaataa aagcaatgct tggatcgcgt ctttatcgcg aacaaaaggt ttaactaaat
     3241 aaccataact ttcttcgaat ccaaacataa aagtatgtga atggtcttct tcaaattgtt
     3301 ggattttttc agcaatgaat ttaaaccctg tcaacacatt gaacatggta gcaccgtagt
     3361 gttccgcaat tgctgtaggc aattcgctgg aaacgattga tttcaatacc gctgcgtttt
     3421 ctggtaaagt gcctcttgat tgatgtgctt ccaaaatata ttgagcaaac aatgctccga
     3481 tttggttccc agtcaatact tcataagaac catctggcaa acgaaccgct gcgcctaaac
     3541 ggtctgcatc aggatctgtt gcaatcagca agtcagctcc ttctttttct ccaagtcgta
     3601 tagcatattc aaaagctgaa tgttcttctg gatttggcga tttgaccgta gtgaaatctg
     3661 gatctgccac agcttgttcg ggaactagta cgaattgttc gaatccagct tgtttcaacg
     3721 cacgttcgcc caacattttc cctgttccat gaagtggcgt gtacacaagt ttcaaatctt
     3781 tccccattgt ttcaaccaat tcacgatcaa tcgtcactgc tttgatttct tctagataag
     3841 cagcgtccac ttcttctccg atgatcgtga tcaacccgct atgtttagct tcttcgtcag
     3901 ataatacttc gacttttagc ggattgctga cttcacggac atatttcgtc aatgcatctg
     3961 cgtctgcagg aggcatttgt ccgccatctt ctccgtagac tttatatcca ttgtaagccg
     4021 ctgggttgtg agatgcggtt accatgattc ccgcaaacgt cttcaaatga cgaactgcaa
     4081 aagaaagctc tggtgttgga cgcagacttt cgaatacata agacgggata ttgtgatgtg
     4141 ctagtgtttt ggctgcttcc attgcaaatt ctggtgactg atggcgtgag tcataagcga
     4201 tagcgacacc tcggcgtttt gtttcttcac cttgtgtatc catgaaacgt gctagtcctt
     4261 ctgttgcttg tcttaccgta tagatgttca ttcgattgat cccaggtcct aagattcccc
     4321 gcatacctgc agtaccgaat tccaacggtg cataaaaagc atcttcaagt ttttcaggat
     4381 cttcacccag atcatttaat tgttttttta agttttcttc taaattttct tcatgtaccc
     4441 attgttcata cacttgttcc caagacatga aaataacacc tctttctgaa atatggttct
     4501 taataagtat agcattcact tattagatat gaaagtatga ggaccaaaaa acgcttaaat
     4561 caagaaagga ttcttttatc aaaaggtgtt ggacgtgttc gcataaatca gattcagtaa
     4621 atttcacgcg aaaaaaaaga gtaaaagaac aattgaaccc gttcttctac tctttttaaa
     4681 cttatgctgt tgtttcttct aaagactcca agtatttata cacttgttgt ccagcaatac
     4741 cgccgtcacc aacagctgta gtgatttgtc gcaaatcttt ttcacgaaca tctccgatgg
     4801 caaaaacgcc tggcatattc gttttcattt cgttgtctgt tggaatccag ccagcttcgt
     4861 tcgtcaaacc tgcttctcta aaaggtgcag ttaatggatc cagtccaaca tagatgaata
     4921 ctccgtctgc tgctaaatca aattcattgc cggttttgac attacgtgct ttcacacctg
     4981 tcactaccat ttcgtttcca acgatttcgt caacgactgt atcccacata aaggaaattt
     5041 tttcatttgc aaatgcacgg tcttggatga tcttttgtgc acgtaattca tctctacggt
     5101 ggacgatcac tacttcagaa gcaaattgtg tcaaatagat ggcttcttct actgctgagt
     5161 ccccaccccc gatgactact agacgtttgt tacggaaaaa cgcaccatca cagactgcac
     5221 agtaagaaac gcctcgtcca gcaaattctt cctcgccagg tacgcctaat ttacggtgga
     5281 tacatcctgt tgcgataatg actgtttttc cttcgtaact tttatcttca cagatgactt
     5341 ctttatggct gccgtgatcc ttgatatctt gaacgattcc ataagcattt tctgtcccaa
     5401 atcttgagac actttcatac atcttataag ctagatcggg gcccatgatc gaatcgaatc
     5461 caggataatt ttcgatttct gccgtattgt tcatttggcc gccaggggca ccttgttcga
     5521 tcatcaatac ggataagttt gaacgtgaag catataaagc agcagtcatc ccagcaggac
     5581 ctgcgccaat aacgattaca tcatacatat gtagattctc ctttacttaa tcaagtaagc
     5641 atactttaca gccaatgcga aaaaaagtct attgttctgc ttgcgtttct cgaatagctt
     5701 tattatactc gaaaaatccg ctgaaaaaca atagactttt ataaatcatt gatcaatcgt
     5761 tcgaaagatt ttctccattt gaatcaatta cttttttata ccagttgaat gattttttct
     5821 ttgaacgggc taatgtgcca tttccttggt tatcacgatc gacataaatg aatccgtacc
     5881 ttttcttcat ctcaccggta ccagctgata ctaaatcaat acatccccaa gtcgtatagc
     5941 ctaatagatc gactccgtct agttctactg catccttcat cgcctggata tgcgctgcca
     6001 ggtaatcgat tcggtaatca tcttccacat aaccattttc atctggcgta tcgactgcac
     6061 ctaagccgtt ttctacaata aacaatggtt tttgataacg atcataaata tcattcatcg
     6121 ttattctcaa gcctaatgga tcaatttgcc acccccattc acttgcttgc agatatggat
     6181 ttttgacaga agcaaagata tttcctgcag tctgttccaa gagttcgttg tcggtagtcg
     6241 aaactcggga agcataataa gaaaaagaga taaaatcaac agtatgttct ttcagcaatg
     6301 cggcatcccc tggttccatt tcgatctgaa tgccatttcg ctccatttct ttcaatgcat
     6361 aagcgggata ttctcctcgt gactggacat caatgaagaa ataattttct cgatctgatt
     6421 ttctagaagc ccaaacatct tcaggattgc aagtgtatgg ataatatgct cctgctgcca
     6481 gcatacagcc tactttgttc tccggatcta cttcatgagc aatcttcgtt gcgatggcac
     6541 ttgcaaccaa ctcatgatgt gccgcttgat atttgatttg ttcttcattt tctccttttt
     6601 caaaatacag ccctgcgccc atgaatggtg catgaaggat catattgatc tcattgaatg
     6661 tcagccaata tttgacaagt cctttatagc gattgaagat gactcgacac agattttcat
     6721 aaaaaccaac gagttctctg gaacgccacg caccatattc tttgatcaaa tgcattggac
     6781 aatcaaaatg cgtaatcgtc acaagtggct caattccgtg cttttggcat tctttaaagg
     6841 catcctcata aaatttcaag ccagcttcat tcggctcttt ctcatccccc attggaaaaa
     6901 tacgactcca ggcgatggac attcgataag tcttgaaccc catttctgca aacaaagcaa
     6961 tatcttcttt ataacgatga tacatatcga ttgcaatttt tgctggataa aaatgatcct
     7021 catcaaattc aaacattttt ttctttccgg tgataacagg aaaacggtct tttccgattg
     7081 gcacaacatc tacgtttgct agtccgcgtc caccttcatc ataaccgccc tcacattgat
     7141 tggctgctgt agcaccgccc cataaaaaat cttttctgaa tcccattata cttcctccat
     7201 tatatgtgta ttacctgata attctcttgc cagttcatct tgaacttcga ttccttcttc
     7261 ttttaatcgg ctgattgtct ccttgaattg cggtaaatac tgctggtgtg caataaacaa
     7321 ctcatccatg atccgttttg ctgtctttcc tgatcgtata agtggattga tcgtaaacgc
     7381 ctgtaaagct gtcccataat caccagtcac agctgcttcg atcgtcagca attccatatt
     7441 cttcattact tgcaaccagc ctctttcagc agcaggaaga gaaccaaacg ctacatttct
     7501 cgctccttgt gcgccaacat aggcagtgac ttctactaca caatctgccg gcaggtcagg
     7561 aacagctcca tcattctttg tagataccac gatctgtgtt tctttatttc catagattga
     7621 agcaatcgtt tcacaggcag cgtctgaata ataagcgcca cctcgttttt gcaattgttc
     7681 aggtttatgg tctaattctg gatctttgta taattcaaat agctctgctt ctgtttcttt
     7741 cacttgctgc gctcttgtac caatcgtttg atattcttct aaagcatgtt ccagcatttc
     7801 ttcttcacga tagtaatagc ggtggtagcc acaagggatc atgttcattt gttttaattg
     7861 ttctttgaaa aacgggatat caaagatatt tttcggtaat ccattctctt catcgtataa
     7921 acgatcaatc aattctgccg tgcggtcatt tcctttatta tctgctactt tgtgccaatg
     7981 gaaatgattt agaccggcaa acttgtagat cagctcttct tttttcttgc caagcattgc
     8041 tggttctgtc atcatcgcca tcactggcac attgcataat ccaatcactt tatcccattt
     8101 gccatatctg ataactgctt ctgtcaccat cccacttggg ttcgcgaaat tgatcagcca
     8161 agcatttgga cataaacgct tcatgtcctc aacgattgcc aaaacgactg gaacggtacg
     8221 aaaagctttg aatatcccgc cagcaccatt tgtttcttgc ccaatcatcc cataataact
     8281 agggatccgc tcgtctttga ttcgtgcatc taattgaccg acacgaaact gagtcgtaac
     8341 aaaatccgca tctttcaatg cttcttcccg atccagagtt gcatagatct tgacttcata
     8401 cggagaagca tcccacatac gctgagacat ctgactaacg atctcaagct tttcttttcc
     8461 ttcttctaca tctacgagcc agatttctcg tatcggtaat tcctcatatc ttttgataaa
     8521 tccttccatc aactcggggg tgtaactgct gcctccacca atcgtagcga ttttgattcc
     8581 ttttgccatg ttctcactcc tttacttttt tacactttca ttatcttttt tgtaaacaaa
     8641 acaaacaata gaaatctgtt tctttccagt aacatttaca aaatttactg ttttctagtg
     8701 gatttttgta aatatcttgt caaaaaatgt ctatt
//
LOCUS       DACCXO010000114         8726 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.117, whole genome shotgun sequence.
ACCESSION   DACCXO010000114 DACCXO010000000
VERSION     DACCXO010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.117"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(306..596)
                     /locus_tag="IVY70_002388"
     CDS             complement(306..596)
                     /locus_tag="IVY70_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP7959499.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(732..911)
                     /locus_tag="IVY70_002389"
     CDS             complement(732..911)
                     /locus_tag="IVY70_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959500.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(908..1258)
                     /locus_tag="IVY70_002390"
     CDS             complement(908..1258)
                     /locus_tag="IVY70_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959501.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(1251..2576)
                     /locus_tag="IVY70_002391"
     CDS             complement(1251..2576)
                     /locus_tag="IVY70_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP7959502.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            3026..3640
                     /locus_tag="IVY70_002392"
     CDS             3026..3640
                     /locus_tag="IVY70_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7959503.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(3805..4047)
                     /locus_tag="IVY70_002393"
                     /pseudo
     CDS             complement(3805..4047)
                     /locus_tag="IVY70_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4251..5570)
                     /locus_tag="IVY70_002394"
     CDS             complement(4251..5570)
                     /locus_tag="IVY70_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAP7959504.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(5725..6312)
                     /locus_tag="IVY70_002395"
                     /pseudo
     CDS             complement(5725..6312)
                     /locus_tag="IVY70_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6950..7207)
                     /locus_tag="IVY70_002396"
     CDS             complement(6950..7207)
                     /locus_tag="IVY70_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP7959505.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(7200..7469)
                     /locus_tag="IVY70_002397"
     CDS             complement(7200..7469)
                     /locus_tag="IVY70_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7959506.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(7600..7779)
                     /locus_tag="IVY70_002398"
     CDS             complement(7600..7779)
                     /locus_tag="IVY70_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959507.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(8160..>8726)
                     /locus_tag="IVY70_002399"
     CDS             complement(8160..>8726)
                     /locus_tag="IVY70_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959508.1"
                     /translation="EEQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETE
                     KYIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATID
                     DYYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTY
                     TYDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT         2673 a         1625 c         1353 g         3075 t
ORIGIN      
        1 atatcgtata tgcattatat tgtaatattg tgtgattatt ttgttttaaa gatatatatc
       61 ggttcggata taaagattat tcatatgtta ttcgaaatta aaataatcac tttttattac
      121 tttaaaattt tttattaatg ataagaaata tgttttgcga gcagcaacta gaataattta
      181 aacattacta aactgtctat attgttacta ataattccct tcttatagtg cattttctta
      241 gaaaaaaaca atattacgat atcatgatat taagatatcg taatattata caattgtgat
      301 tgatattata tatgtttttt tattttttta tcatgtttaa tcttgaagga atcatattct
      361 ttttctaaag acaggagctt ttcataaaca tcagacatgt ttaaactaga ggccaggctt
      421 aaatcataaa cgaatgaagc aggtaatgat tttattctac gttgtcgggt tatccagctt
      481 gcaaccgttc cttgtgggta ggcatataac tcacaaaatt gttctacagt gattcctaaa
      541 ttcttgataa tgtaaacatt gatcgggtgc gagtaaacaa atgtattgat agacatggtg
      601 cgtaagccta ctttctttta ttattgcgtt tgcaataata gtttaacaca cgcattattc
      661 tagtatctac tcctatttgt aaaaacaaaa aaggattcat agagataact ctatagaatc
      721 cgtaaaatac cttatatata taacgggaag atataaaacc taaaattatg ataacctgct
      781 gcagcttcta catagaaaga gaagcgctca acaccaacct catttatcaa acgttggcca
      841 taaccccaaa tctcttctga tagctgatca aaggttatat actttagctc atattgctgg
      901 ataatgttca ttctaatcga ctccatttat tatcctggta aaattcgata tgacgtattt
      961 cgtcgtagtg aattttgtct tttccaacgt agatccccag ctcatcacta cctaagatct
     1021 tgccaatgat atcttctgga tagtatcctt cagcgtttcg ttcttctttt tgaatagata
     1081 ctgatctatt ttttatgact gcagcgttca atacttccgc aatctcttca gtcgtcattt
     1141 gttcttttgc taaattttga ctggttcgtt gttcattgtc ggcatcaatt tcttcagtat
     1201 gatcagataa ataaaaccct gcccacttta aacgtccacg atcattgtaa tcaatcattt
     1261 ttaattcccc catttccgcc cgcatgacca ccaacaagtg tgcttctagt aatgcttcta
     1321 gcgccttcta acattgaaga agcatgtaca atcgacgtaa aaccatattt ttgccgaatc
     1381 ttgtcaataa ttttatctaa attttcttcg tcaatttgct tttgtggatc actaaataaa
     1441 tccagctgaa gcgaatcggt gtacacaagc ttgctatagg ttattccaac atgccttact
     1501 tcttgtcctt cataatactt atcaaacaag aaaagacaat aattctgcaa ttcctttgta
     1561 ttatctgtga tagggatctt catctggtgt gaaaaccctg gtctcgtttc taagatcgac
     1621 gtgccaatgt gtaaatggac acaacctgtt ttacagttat gctgcctaat gcgtatcgct
     1681 acttgttccg ccatttcttt taccaccagc tcgatttcat ttcttctagt ataatctcgt
     1741 ggtaacactt gtgaatttcc atatgatttt tcctttgtcg gttctggtgg gattgctata
     1801 tctgttcgat cgatcccatt agcatggaag tacagctgta agccaatcac acctagtcta
     1861 ttcttaatcg tataaggatt ccagttagca agatctctaa tgctcataat ccccatctga
     1921 ttgagtcttt ttttcattcg agagccaatt ccccaaaagt ccgtcatttc aggaatattc
     1981 catacttttt caggaacgtt ttcgtacgtc cattccgcaa caaatccttc attatgcttt
     2041 gcttcgttgt ctaaggctag tttagccaaa aggggattat ctccgacacc tactgtagca
     2101 atcaatccta gctcttcttt aatattctcc tggatcattt gagccagctt ctttctccgt
     2161 tgacttcgtg ttccttcagt cgtaaaaaga ttcagtgatc gggtcacttt taagattgat
     2221 tcatcgatcg agtaaatcag tagatcttca tcagccacat atcttctgaa aatattattt
     2281 acctgcatat ttcgcttgat atataggttc atacgtggtg gaacaacgtg tagtgtttta
     2341 ggaaatggtt gtggtaagtc acgtggtcta ctcacatttg taataccata ccgctttttt
     2401 gcttcaggag aagaagctaa tatcaatcct gaaccagtat tatcagctcg actcataaca
     2461 acaagttctg ttgttaatgg atctaaattt ctttctatac attcgacact tgcgtaaaag
     2521 cttttcatat cgattaaaaa gtaatcattt actggctctt ttgaataatc catcataaat
     2581 aacactccat tctttcacat tatacaaaca tatgttcgat attgtaaaga agaaatagct
     2641 aaatacataa aaaaagaagc ataggtatgc tatcaccctg cttcttcttt tgatttgtat
     2701 gatcaattta ttctatcact ttactaatta atttgaaaat ctaataaaaa ctctcactct
     2761 aaaagagtga gagactgcaa attagagtac ggactttttt ggagttccgt tctttaatat
     2821 taacaaatga ataaaatcct tacaacattt gagaataagt tatcagtaga cacttacaaa
     2881 aaattctcat actatttcct ccatcattag tttttgtcta tataaggttt accctaaata
     2941 gacactggta taacattgaa aaatatggct tcctcgtgta tgattagggt gtattttaaa
     3001 tagttgcaga aagtaggtaa aagttttgag agttgcatac atacgtgtct catcgatcga
     3061 tcaaaatgaa caacgccaga ttgaagaaat gaaaaaattc ggtgcagaga gaattttcat
     3121 cgagaaacaa tctggcgcaa cgattactca tcgacctgtt ttccaagaag cattagatta
     3181 cgtccgagat caagatattt ttattgtgga agctattgat cgacttggaa gaaattatga
     3241 cgagatcatc gcttcagtca attacttgaa gaagaaaaat gtgaagctga tcattactag
     3301 tttaccgatc atggctgaag caattggcaa tcctctcctg gataaattca ttaaggatct
     3361 gatcatccaa attttggcca tgatcgctga acaagaacgg actgaatcga aacgtcgcca
     3421 ggcacaagga attaagatcg caaaagcaaa cggcgtctac aaagggcggc ccaaattata
     3481 cagtgcagac gcaaaagatc cccaacgtcg ccttgtttac aaaagcatcg ttgaagatct
     3541 taaaaatggt gttgcgattg ctaagattgc taaagactac aatgttacga gacaaaccgt
     3601 gtatagaata aaaaaagaca gcatggtcaa tgacaaatag ttgatcatgc tgcttttatt
     3661 atcttctcat ctttaatttc ctacatataa ttactatcta taaaattaag tcttactcag
     3721 aacagataaa gcataaaatt ccaacaaacc attttttact taactaaata aagagtctgt
     3781 taatataatc ttcacttatt tacgttaatc ccctactgta tttgttggtt ccttatctat
     3841 attttctaca aaatccttat tatcttgtga tttagcttcg tataattctt tgtatgtcga
     3901 cacattggtt atactaattc ctatagctag ttcttgagac tctacttttc tctctagctc
     3961 ctttgacaaa gtgatttgag atttatttgg ttccgttgga gtcaattcta tagattcttc
     4021 ataaaaatct ctatacataa agctatccct gaataattca tagctttaat tctttggcac
     4081 attttattga aatatgtatc actaatatcc ctatcaactg attttgacta aaaagttact
     4141 tcaaacattc actgttcaaa aatattgagg aataaatttt gggtgtaagg cattttgagc
     4201 atacttctcc ctggataact tcccagtaaa aaaatcgtta gattgttgga ttagatccat
     4261 tgactggctc gcctcaggcg agcaaagacc ctgtagaatt cattctgata cacatgataa
     4321 ctagataatt tgagatagac tcgtctaccc gtttggacta atttcccagc aactttcaga
     4381 aacttcaatc gaatagaatg aatggtcatt cccttttgga cttcttcaaa gccaatcgtt
     4441 cttaagaagt tgactaagtt gtaagctatc aagctgagca tcattcggac atgattctcc
     4501 aaaaagcgag gactgtctgt tttgtcaaag taaaagccag ctttcgcttc tttaatgaag
     4561 ttctccattg tgccacgttt ggcatagata gaaaagatgg tatcaggaga aacattttct
     4621 gatagattcg tcacgataaa ttcatgtcga aagagtagtt cgcccgcttc acgtgttgag
     4681 cgtatacaca cgcgacgact ttgtgaccag gattgtgctt gataagtggt cgagaagtac
     4741 tgaacttctc gttcttccca cttttgatta tcgccataaa gtactgattt ctcagctatt
     4801 tgacctagtc tacgattatt cttcagtcga ataacataat gactcttttt tgattcacac
     4861 gaatcataca caccaggtgt agcgaacccg ctgtctccac gaaccaagat gtcagtgttt
     4921 ggtagagagt gattatagtg ctctaataaa ggtgtgagaa actcctttac gccttttgat
     4981 gtatattgat ttcctgaacg tagttcagct tttaagaagt caccagtcaa tccgtcaaaa
     5041 gctaccaatg gatggtaacc ataggtttgg tagtgtgtat tataattcgt ttgttcttgg
     5101 tgaccaaatg tatctgaatg ggtcgaatct aaatcaatga ttaattccgt atcattacgg
     5161 atgaggcgtg ctttatcgat aagttcttgg ttcaaagctt gaagttcatg gatattctcc
     5221 tcagatagtc gatctaaaaa tcgggaaagt gaagattgag aagcgagttc tttcctatct
     5281 aaaacagctt taaacacagg atcttgtctc aggagattag ctgcagaatc agctgagtaa
     5341 ccagcaatta attgcataat gaactgctca agtatggata agttgtcatg agtaaaatac
     5401 gctcgttcgt cttcaatgtg aatgtgttgc ttagccagtt cagaaaaacc aaagatgttc
     5461 atcagctctt tcactaggac gagacccgaa tcactcgata attgaccacc tgtatgagaa
     5521 atagtgatat ttgaattgaa ttttacttgg tttttgtgta agctagtcat gaaaagaact
     5581 cctttctttt ggttggttta gtcactttaa ccatagcaga aaggggttct tttttcatca
     5641 cttaacgggt gaagatgaaa aagtaattgt aagctgccgt gaggcagttt cacatggttt
     5701 taattaaaag tatgaattat tcaggctatc attaatttta taaaaaaaat taaatgtttt
     5761 tagcactgcc atataacatt caaaacattc ttctatagtt ggtttacttc cttgaggaga
     5821 atgaaccaat ttattacgtt tttctatgaa atttttaatt tcctttctta tttctttcgg
     5881 aaactcaata ttaaagtacc ctttaaacat gctccctaat aatttgtcta cagggggaga
     5941 ttgtaaattc tcaaatatta gagttaaatc aggtttctct tgttgataaa attcctttac
     6001 ccctatttca caggctgtaa ttatcgaaat aaacctatct ctagtatttg atatattttt
     6061 acttctttta aggagtataa gagaaaaggg gatactttcg tgtccatgca tgtaattaac
     6121 taattgttga gcttcagaag ttatatctct aattatatag ttaacggaat taggaagtgt
     6181 ctttaactct aatgaaattt ctatccaatt actatcttct aatctgtata tgctcgaagt
     6241 ataatttgat tcgtcagcaa gcatcgctac atcccaaaca ctgatcataa aatcttttaa
     6301 acgttcaatc atttcagcaa tattgctaaa atcagctaat ttttctttag aataaaaaaa
     6361 aacatttata tcagaatcaa taaattctat ttttttatta ctatatcgtc atcattatct
     6421 tgaccattta tgtttattgt tccctgagtg gggagtactt ctttcaaatt caagttaaat
     6481 ttatattcca ttacttctcc tccaatatat aaaaattact taactgtatc caaatttaat
     6541 atgtaaataa atcattaatt tttagctaaa ttctatgaga agtttatttt tgttgttttt
     6601 gctaattaac taaaactagt acaaaaaatt taaaatagat tattaaacct attgcttatt
     6661 ctattcagtc atatatataa gcgtcatcct ttagtaacta acatcccaca ctacaatagt
     6721 attataacat atctattcaa cttcaaattg ttgaaattta agccatttat atttactttt
     6781 cctcaacttt agaaatgatt ttactaacct tatatccgta atacgcgtaa caaacacatt
     6841 cattaataac aatcaacact aagctatatt agctttttaa atgactaggg taaataaaat
     6901 aaggatcaaa aacttttaag tttctgaccc tttccttact tccgattggt taatagtgat
     6961 cttttgcaga ataaataaat atcgtttcat tttcaactct atatatcagt ctatgttcat
     7021 ctgtaattct tctggaccat tttccagata aatcatgctt taatggctca ggttttccta
     7081 atccagcaaa gggggaacga tcgatatctt ttattaactt gttaatcttt tttatattgc
     7141 ttttgtttcc ttgctcatgc caataaagat aatcatccca agcatcatca gaccaagcct
     7201 taatcatcag attcaacctc gattaagtca tgtgttttaa atgcaccttt ggagaattgt
     7261 tcatctcctc gacgaatttt ttccatgacg taattattag aaagtgttct caacgtttct
     7321 tgcatagaat cataatctct ttttgataat acaacaactg tatcttctac atctttactt
     7381 gttacaataa gtgtttcagc atcctcatta acttgtttca tataactacg taaattttgg
     7441 cggaaatttg aataagctac tgcttccatt cctttcaccc cttatttatt tctataaaac
     7501 aattgtacat tatattgtac aattaagcaa atgattaata ttctaacttc ttataattaa
     7561 aagtgctatc acttttagtt tttaggataa aaagtgacgt cactttttga gtgctggtac
     7621 ctcaaatcct gctttttctt gggcaatcaa taaatctaac gcaatacaca taatcctgga
     7681 ttcacttttg ccgtacttct gctttagttg ctgcaattct ttttgtctct tttcatccaa
     7741 cgtaacgggt aaccgtttat ttcctattgc aattggcatt agtactctct cctttcatcg
     7801 aaataaccct atgaaaaata tggctgcaat catcttgaat agtagactca caagggctaa
     7861 tacaaagcgc caaaagatca aacaaaatac cagtattgcc aaaaatacta cgaagtttaa
     7921 atgcacagct gcagcatggc gcataagtat ttcgccaaca aataggacta acaatagagg
     7981 aatgatttta ttcatatgga tctccttctt tctcttaggt tatcgggtgt tgcccgctta
     8041 tttcgcacca cctaagcggc gggggcggtt ggagcgattg gtgcgaaata agaaccattc
     8101 ccttagttac taggtttttt gtccttattt cacacattct cacttttcgg tataaatgat
     8161 tattgccttt ttttcctttc aaatcgttga tatggcattc gtttacggtc aagttcattt
     8221 tggatttctt ctgtatcgtc gtaagtatag gtgtctaaaa aatcagttaa agtcgtgtac
     8281 cagtcgtcgc ctgggttatc tacttcttct acatattccc tcaaaaatgc ttcgggtaca
     8341 gcgagttcat aatccattcc cattaacacg atagattccg ttccacacgt caaaaatcta
     8401 gtaaatacaa ggtaataatc gtctatcgtc gcatctttgc tgtttagctc ttccatttct
     8461 tcttccatta attccatgct ctcaaaaatt gaataatatt ctgattctga gtaatagtgt
     8521 ttcctgcaaa tcgtgtagct gacaaagttt tcgaccagct cactcctttc taggtaaaca
     8581 ttcatgatgt atttttctgt ttctagtcgt agaagctccg ttcctttttt taccgtttca
     8641 ggacgacgtt tatacacgtc taaacgatta tggctgactg tgaccttcgt ttgactcgtt
     8701 tcactttcca aatacaattg ttcttc
//
LOCUS       DACCXO010000115         8686 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.118, whole genome shotgun sequence.
ACCESSION   DACCXO010000115 DACCXO010000000
VERSION     DACCXO010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.118"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            85..987
                     /gene="gnd"
                     /locus_tag="IVY70_002400"
     CDS             85..987
                     /gene="gnd"
                     /locus_tag="IVY70_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP7959509.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            989..1990
                     /locus_tag="IVY70_002401"
     CDS             989..1990
                     /locus_tag="IVY70_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP7959510.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1987..2271
                     /locus_tag="IVY70_002402"
     CDS             1987..2271
                     /locus_tag="IVY70_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7959511.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2304..3101
                     /locus_tag="IVY70_002403"
     CDS             2304..3101
                     /locus_tag="IVY70_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7959512.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3115..3948
                     /locus_tag="IVY70_002404"
     CDS             3115..3948
                     /locus_tag="IVY70_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7959513.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3966..4823
                     /locus_tag="IVY70_002405"
     CDS             3966..4823
                     /locus_tag="IVY70_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7959514.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4845..5354
                     /locus_tag="IVY70_002406"
     CDS             4845..5354
                     /locus_tag="IVY70_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7959515.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5391..5792
                     /locus_tag="IVY70_002407"
     CDS             5391..5792
                     /locus_tag="IVY70_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959516.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5904..6257
                     /locus_tag="IVY70_002408"
     CDS             5904..6257
                     /locus_tag="IVY70_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959517.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            6291..6734
                     /locus_tag="IVY70_002409"
     CDS             6291..6734
                     /locus_tag="IVY70_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7959518.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6751..7836
                     /gene="uxuA"
                     /locus_tag="IVY70_002410"
     CDS             6751..7836
                     /gene="uxuA"
                     /locus_tag="IVY70_002410"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP7959519.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7829..8542
                     /locus_tag="IVY70_002411"
     CDS             7829..8542
                     /locus_tag="IVY70_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7959520.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         2913 a         1362 c         1966 g         2445 t
ORIGIN      
        1 aatcaaacaa aaaatatttt tgtaaaatct atttacaaaa aaattttttt gtataatgag
       61 ggtataagaa atggaggcgc tatcatggaa attggtgtaa ttggtttggg aaaaatggga
      121 ttgaatttag ctttaaactt aaaagagcac catcacaaag tacaaggact cgacatttca
      181 agtacagctg ttagtcaggc aagagaaaat gacattcctg cttatcaaga gatctctgaa
      241 tttctagcta cttttgagca gaaaaaaatc atctggttaa tgcttcctgc tggagagata
      301 accgaaaaca tgttaagaaa actttttcca aaactcggac caggagacat tattattgaa
      361 ggcggaaact cttattataa agattcaatc cgtcgagcag aagagtttca gaaaaatgaa
      421 atcggttact tggattgtgg gacttctgga ggaatcgaag gagcacgaca tcaagcctgc
      481 ttaatgattg gaggagataa agaaactttt ctatccgtgg aacaattatt caaagatgta
      541 gcaattgaaa gaggatatct gtatgctggt gcatccggaa gcggacactt cttgaaaatg
      601 atccataatg gaatcgaata tggaatgatg caagcaatcg gtgaaggatt ccaacttgta
      661 caaaaaagtg agtatgcttt tgacttggaa caagtcgcac gtgtttggaa tcatggttcg
      721 gtgattcgcg gctggctgat ggaaatcatg gaagatcagt taaacgaaca tcccaattta
      781 agtaattatc ggggaatcgt ttctgcatca ggtgaagcaa aatggacggt tgaaacagcg
      841 ctagaaatgg ctgttccagt tccagtaatt gcaactagtt tgttcatgag gaatctttca
      901 caggaagaag atagtttttc agcaaaagta gtttcagcac taagaaatgg gtttggcgga
      961 catgaaatag ttgaaaaggg tgagtaaaat gccagattat catatagcaa tcgttaattc
     1021 cagcagtttc ggaagagtct ttccacacca cttggaaaga ttaaagtcag ttggagacgt
     1081 tcatttcttt actgttgatc aagagattcc aggaaaagaa ctagcggagt tacttaaagg
     1141 attcaatatc attattgcaa gtgttacgcc attttttcga cgagattttt ttgaaaacaa
     1201 agatgaactg ctattgattt cccgacatgg tattggttac aacaatattg atttaaaagc
     1261 agcaaaagaa caccagacaa tcgtatctat catacctgct ttagttgaac gaaatgctgt
     1321 agcagaaaat aatataacta atttgttgtc catgcttcga cgcacgacac aagcacaagc
     1381 aagagtcaag gacgatcgat gggaagatcg ggcggagttc gtcggtagga cgctgtttaa
     1441 caaaacagtt ggtgtgattg gtgcaggaaa taccggaagc ggagtaatcg aaattttgcg
     1501 taacggtttt cgctgtgatg ttctagctta tgatccttac aaatcagatt tatacatcga
     1561 aagctttggt gcaaaaaaag tagaacttca agaattactg gaacaatcag atatcatttg
     1621 tttatgtgcg aatttgacag aagagaacta tcacatgatt tctgataaag aagtcgatca
     1681 gatgaaagaa aatgtttatc tatctaatag tgcaagaggc gctctagttg atgaagaagc
     1741 gatgattaat ggtctgaaaa gcggaaagat cgcaggatat gccacagatg ttttagaaga
     1801 agaaccggga cgtcaagatc atccgtattt agcgtttgaa caagtagtga ttacgccaca
     1861 tatatcggcc tacacaaggg aatgtttaga agcaatgggt gaaaaatgtg tcagagatgt
     1921 cgaacagatc agtcagcacc ttcttcctga acgttctgtc cagacaaaga gcaactatat
     1981 aaaaggatga tgaagaaaat ggtgaacggt ttaaaagtga aaacaggccc tcaattttat
     2041 ttatatgaag aaggtggtat ttcaaaagtt tctgatctct tgaaatcata cggcgcgaaa
     2101 agagtactgg tcacccacgg aacggtatca tgggaaaagg cgctacctaa attagtattt
     2161 ttgaatgacg aaacgattca atttttttat catcgttatt ctggagaatg tagttatgct
     2221 gaagccagac gaatagctac aatcatcaaa aaaatgaaat cgatttcgta gttggcgttg
     2281 gcggaggaaa actgaccgat ctagtgggtt atagtgccaa tcttgcaaga atcgatttcg
     2341 gagtcatccc tactttagct agtaattgtg ctccatggac acctcttgct gtcatgtata
     2401 aggagaatgg acaagcagaa gggaaaacag aacactttta cagacaagct gcttttttta
     2461 taacagatcc ccaattagtg attgattccc ctgttccata tttcattgcc gggttagcag
     2521 atacactagc aaaatggtat gaatctgagc tcatcttaga acaagatttt ttgcaagatg
     2581 aatcattttt gaagctagca caagatactg cgaagatctg caaagaagag attttgaatc
     2641 attctgctaa agcaatccaa gatatggcac aaagaaaact gacatcggag ttcaaacagc
     2701 tttcagagat tatctttgct gttgctggtt tagttggagg attaggagat aaatacgcaa
     2761 gaaatgtggc agcacatgct atgcatgacg ctatcagtaa atatattccg gaaagccatc
     2821 gttttctaca tggagaaaaa gtagcgtatg ggattttcta tcaactggca ctagaaggtc
     2881 gctgggaaaa aatagacgag ttatatcctt tgtatcggga attagaactg ccggtttctc
     2941 tagaacaaat gggattattt ccgaaagata aacagataat agataaaatg gctgaattta
     3001 ttgattcgaa agagaaagtg catttgattc ctatagaggt aacacagaaa tcactgatac
     3061 agtcaatgat ggaactggaa ggttatatca gaaagatctg aaggaggagg acacatggaa
     3121 acattaagtg tagtacaaag tttgttgatt gctttatggg tagccgcggt tatgtcccgc
     3181 tggttaggtg gaggagccac gttgacctta cgtttttctc ctttgatgac tggattagtc
     3241 gtcgggttga tcatggggga agttgcacaa gcgatgattg ttacagccgc tttgcaaatg
     3301 atttatatgg gggttttttc tccaggagga tcaatgccag ctgaaccatc tattgcagca
     3361 gctatcgcag taccagtcgc attattaggg gatctgtcgc cagaagcagc gattgccgtt
     3421 gcggtgccag tcggattgct cggaagttac ttgtatcagt ttcgtttttt catcaatact
     3481 tttttaggta aatatacaga caaagctgta gaagatttga atacaaaagc tatggcccga
     3541 tcaattattc tttatccaac ggttgcctca tttttattat tcgttccgct tgttttcatt
     3601 gctttatatt taggggcgcc attgatcgct gatgtaatca aagctttaga gggaacagtc
     3661 gttatccaca ttcttgaagt agtaggtgga gggttagcgg caatcggtat cgcgacaact
     3721 gtatacgtta tcggtcgaaa agattatctg gtctttttct tccttgctta ttttatgagt
     3781 gttgttttga aatctttaga tattacgatg gttacttatg ccatttttgg gattattatc
     3841 gctttgatct ttgtccagtc gcaaaaaggg aaacaagtac agcctgccac aagcaataac
     3901 aattcagcat cttttgatga cgaagatgac tatgatgacg gattttaatt taggaggaat
     3961 gggaaatggc tgaaataatg gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag
     4021 atgtaacaaa agcctatctg agatggcatt ttgcaaacga aattcctcac tcttttgaac
     4081 gttacttggc gccatcacta ctatacggaa tgatgccaat cttgaaaaaa ctttataaag
     4141 atgaagactc tttaaaagca gcatataaac ggcagttact gttcttcaac actcaattaa
     4201 gctggggtgg cggtgtgatt actggcctaa tgtcttctat ggaacagcaa agagcagaag
     4261 aagagtatga agaaaaagag atcttgatgc aggatgactt gatgtataac acgaaagcgg
     4321 gattgatggg ggcactagca ggaatcggag atgcaatcga ttcaggaacc gtccaatata
     4381 tttttatcgc aatcgcggta ccttgggcac aacagggaag tcctttaggc gcactttttc
     4441 cttttatctg ctttgggttg tatcaggttt tactaggggt atttttcgca agacaagctt
     4501 tcactatggg ccgtaatgct acaggcctaa agcaaaacac tggtgttcag acagcgattg
     4561 aaatgctatc agttttaggg ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag
     4621 tagaatcttc attgaaattt gcgatatccg gacgtgagtt tgttatccag gaaattcttg
     4681 atcaaatcgt gccagggata ttacctttag ctgtcgtaat gggtgtgtac ttgttctata
     4741 cgaaaaaagg attgaaagtc acacaagcat tattatggct gactggaata ttaattgtgc
     4801 tagcaggaat cggtatttta taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt
     4861 tagcgcgtgt ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa
     4921 gtggagtgaa ttccattttt gttgtagacg aagttgtagc aaaagataaa tttatgagag
     4981 atctttacaa aaatgcagga agccgtacag gacaaaaaac aattgtcatt acaccagaga
     5041 aagctaaatt ctattgggat gaatatcaat ttaaagaata caattgtatt ttgatcgcaa
     5101 aaacagtcca aacgatttac gacttagtaa aacacggaat ccccatggaa gaattgaata
     5161 ttggagggat tgctcaaaaa aatcctgata cagatctttt agtgacgaaa tctgtttatt
     5221 taaataaatc agatgctgaa aaattgaaag aaatgaatga acaatatggc gtaaagaata
     5281 tttattttca agccactcca tctgcaccta aaacaggatt aaaagatgta ttgaacaagt
     5341 ttgatttgtc ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga
     5401 acgagctgtt tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa
     5461 aaaagagtaa acagcgtttt ctttcagcat tagtatcaga tatttacagt atgagaacag
     5521 atgtgacagt cattgcttac gatacgctag cctaccgatc aaaaaatatt tatgttggag
     5581 atatcgaaaa agcagagaaa gttatctgta cttattacga tacacccgtt catgcattag
     5641 gaagttattt catgtttgat tggaaagacc aaaggaaaaa aacaatctac tctatattat
     5701 tgtccttcat tttgttgttc agtttaggtt ggtggggaat gatgatatat aacaaaaatc
     5761 ctcatcacgt attcgatctg ctatccgtat aaacaggtat cactgtacta gcatttggga
     5821 gctacttttt cttattggga aaagcagctc gcggatggcc tagcagacaa acttttattc
     5881 gaaatacttc atccatatta acgatgctag aaatgatccg tacgattgat gatccgagtg
     5941 tagcgtatgc gtttgttgat gaaggctgtt atggagaaaa gggcttagac agcgtgagat
     6001 cgggtatgaa aaaagaggct attctttttt atctagactc tgttggggca gatacaccgc
     6061 tacagttttc gggaaattat ttttcaaata aagaacagtg gctgaaacaa gttgataagt
     6121 tgaaagaaaa aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg cagttcttct
     6181 atttgaccaa aacagattta cgagggaaaa catttaattg gcaaaatgcc aatcagatta
     6241 ttgctctttt tcgataagta aaaagtgcaa aacaaggagt gagaagacta atgttggaaa
     6301 tcatgatcgt tacacatggt aagttgagcg aaggactgaa agatgcggcg gaagtaattg
     6361 tcgggacatc gagccatatt gaaacaatgt gcttagagtc tggggaggac atccaaacat
     6421 tcggagagaa ggtcaaagaa aaattaacta tgatcaacac agcagaaccc atgctagtat
     6481 ttgttgatct aattagcgca agtccgtaca atcaagcgct cttggcagtc aatgaactga
     6541 ctgaagaaaa gaaagcagcc acttatgtca tagggggcgt gaatctgcca atgttgttag
     6601 aagcaatcaa tcatcagatg cttcacacac cgatagaaga agcggtggaa caaattgtca
     6661 aacaaggaaa agataattta actgtttggc atgcttcagc ggaagaaaaa aaattagatg
     6721 aggacgattt ttagaaggaa ggctgaaaag atgaagtggg gatttagatg gtatggtgaa
     6781 aaaggggatt cgatcccatt aaaatatgtt cgccaaatac cgggaataaa tgggataatt
     6841 ggaactttgc tgactaaaat gcctggagat gtttgggaga tttcagaaat tgaggagttg
     6901 aaagcatctg ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt agcgatccac
     6961 gatgcaatca aggcagggac agatgaacga gattactata ttgataacta tatacgaaca
     7021 atcaaaaact tgtctcgatg tggggttcga ttgatttgtt atagcttcaa accaattttt
     7081 ggatgggcga aaactgattt gtttaatgaa aacaacgatg gaagctattc tcttttatac
     7141 gatcagaaaa tcgtcgatca gatggagcca ggagaaatgt atcaattgat acacagccag
     7201 tcaaagggat tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg
     7261 gaaaaactgt atgaaggaat gaccgaagaa gaattatttg aaaatctgac ttatttttta
     7321 gaaaaagtga ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca tccagatgat
     7381 ccgccatggg aactttttgg ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa
     7441 attctaaatg cagtagattc tccatataat ggactaacgc tgtgcacagg atctttaggg
     7501 gcaaatccgg aaaacgattt agttgaaatc atccatgagg taggttcgcg catccatttt
     7561 gtccacttca gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac ggcacatccc
     7621 tctacagaag gctcccttga tatgtatcat attatgaaag cgttggtgca agaagggttc
     7681 gacggtccta tacgccctga tcatggtaga accgtgtggg gtgaagtggc tatgcctgga
     7741 tatggcctgt atgatcgtgc aatgggactc acttacatgc aaggtctata tgaggcgata
     7801 agtaaggaaa aaccactggg agagcattat gaataaaatc aagctcttca tctttgatat
     7861 ggatggcctt ttattagaaa ctggacgttt agcttatcgt gcttatgtaa aatcagcaca
     7921 aaagtatgat tatgaaatgc gtaaggaagt ctattattta ctaactggcc aaacagaaat
     7981 ggcgataaga gaacaaatgg gcattttgta cggagaagat gttccatata tcaaatggcg
     8041 agaagcaatc aatcaatata aagaaaaaat cgtgaaagaa gataagagag tatatacgaa
     8101 aaaaggagca gaagaaattt tatcttttgc aaaagaaaga ggaatccata caatcgtggc
     8161 ttcgtctaat acacgggtaa aagtagaaat gtatttaaaa atggaaaatc tatatgcctt
     8221 gtttgatcaa atcatttcag gggatgatgt aaaaaaagga aagccggaac cagagatctt
     8281 tttgaaagct tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat ttgaagactc
     8341 gattgcaggg atcgaagcag caaggcgagc gggcattttg tcttttctca tcaaagatca
     8401 tttgataggg ttgcccgatc ataatgggaa gcataagttg aaagtagatg taagtttata
     8461 tgaatccaat aaagcagact atgaatttta tgacctgcat caagcaaaac gatttttaga
     8521 aagtaaaggg ttgtatctct aataaagagg actattttct agtcctcttt atttattgtc
     8581 ctgcacgact gagggaacag atgaaaaaga agtaattttt tattttgaag tatctacctt
     8641 atttgtagtg aacaatttag aacaaacaca aaacgcatct actgtt
//
LOCUS       DACCXO010000116         8643 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.119, whole genome shotgun sequence.
ACCESSION   DACCXO010000116 DACCXO010000000
VERSION     DACCXO010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.119"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            157..936
                     /locus_tag="IVY70_002412"
     CDS             157..936
                     /locus_tag="IVY70_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959521.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
     gene            977..1921
                     /locus_tag="IVY70_002413"
     CDS             977..1921
                     /locus_tag="IVY70_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7959522.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1937..2716
                     /locus_tag="IVY70_002414"
     CDS             1937..2716
                     /locus_tag="IVY70_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP7959523.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            2729..3427
                     /locus_tag="IVY70_002415"
     CDS             2729..3427
                     /locus_tag="IVY70_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP7959524.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3455..4219
                     /locus_tag="IVY70_002416"
     CDS             3455..4219
                     /locus_tag="IVY70_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP7959525.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYKQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEIKKKKFGLF"
     gene            4232..6049
                     /locus_tag="IVY70_002417"
     CDS             4232..6049
                     /locus_tag="IVY70_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7959526.1"
                     /translation="MFVLTRRRKIATLVVVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISTGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASDVSSILILLFINRQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIQVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAKGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNK"
     gene            6063..7286
                     /locus_tag="IVY70_002418"
     CDS             6063..7286
                     /locus_tag="IVY70_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HAP7959527.1"
                     /translation="MNKLEKRQIPFSPPDITDLEIKNVVEVLKSGWITTGPKTKKLEK
                     KITKWLGTSKVVCLNSQTAAAETGLRLLGIGENDEVIVPAYTYSASASVVCHIGANLV
                     LIDCQQDSLEMDYDALEAAITEKTKVIIPVDLGGIPCDYDRIFEIVEKKRNLFQPSNE
                     IQKAYGRIIVMADSAHAFGANWHDQMVGNVADLTTFSFHAVKNFTTAEGGALVWKEHD
                     KLDNEAMYHQCQLLSLHGQSKDALTKTQLGAWEYDIVGPWYKCNMTDVTASIGLAQFE
                     RYPNILKRRKNIIKRYDEAFQQLGIEVLMHYTDDYSSSGHLYITRVPNISSEQRNEII
                     IKMAEQGIACNVHYKPLPMFTAYKNMGFDINNYPNAYAHFVNEITLPLHTKLTDEEVE
                     YVINNYSKIVKDLVG"
     gene            7300..8034
                     /locus_tag="IVY70_002419"
     CDS             7300..8034
                     /locus_tag="IVY70_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006443581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP7959528.1"
                     /translation="MRKWEDLPKEMQNDEIRYYYDILSKKKFSLVVKRVFDILVSATL
                     LLTLSWLFFILAIVIKIDSPGPVFYRQERITRYGKKFKIYKFRTMVINADKDGTLVTV
                     KNDDRITRVGKVIRKYRLDEISQLINVLVGDMSFVGTRPEVGKYVKSYSPLMLATLLL
                     PAGITSETSILYKDEDKLLHDKNNIDKTYIEKILPSKMYYNLKSISEFSCMNDLKIMC
                     KTIFVIFGKEYHADLEGEREFINQIK"
     gene            8051..>8643
                     /locus_tag="IVY70_002420"
     CDS             8051..>8643
                     /locus_tag="IVY70_002420"
                     /inference="COORDINATES: protein motif:HMM:NF012792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7959529.1"
                     /translation="MVIEEIKSSDKELINSIVDIHMRTFTNFFLTFMGKKFLELMYRS
                     YTEYNDSGILVAIQNDKPIGFLAYSGDMSGLYKYMIKKRIIPFGFYSCVAFIKKPNIL
                     MRLFRAFLKPSETKRDENYVELASIGVNPKEKSIGVGSMLIDNLKSRIDFSKYEYIAL
                     ETDALNNDVVNNFYKKNEFILERKYSTNEGRIMNEYR"
BASE COUNT         3055 a         1448 c         1779 g         2361 t
ORIGIN      
        1 aaaactgttt gtggtatgat tatataggga aaacctgttt ataaatattt ctatgaatat
       61 attagtttaa ttatgctttt aaaataaaaa gcgctcattc tgatgtgaat gaatcacatc
      121 tttatttatc tatcatcaat atttaggagt gaatctatgg aaaagccgtt cgtcaagtta
      181 ttggctactg tggcggtagg cacaggcgct attgtgattt gcttgtttgg ataccatctc
      241 aataatcagc ggcaacatca tcaacggatc aattacgcag aatctgccat caccaatcaa
      301 aaagacaccg tgacctcttt atcaaaagaa gtagataagc tgtacagtac aaaagaaaaa
      361 attttcctaa atcctgaaat aacagaagaa acagtttcaa atttgtcaca taaactatct
      421 tctatcaaac tttcagcaga tgattatgac atcaaggaat cagaattacc taaagaagct
      481 gctgcgatcc aagaagaaaa gaaagcagtc ttaacacagt tagaggatgc agaatcaaaa
      541 ctgaagatcc aaacggctgt caataaatta tttactaaaa acgtatcaaa ctggcagcaa
      601 gctgtggatg atgtcatcat caaagaaaag ttggcaagtg cagatgtcgc ccatgtacgt
      661 gaaaatatga gcttttttaa ggatagtgct tggaaaacgg tcgttatgca atatctagga
      721 ttcgctgata cccaaattgc acaagtcacg caactagatc agctattcga tacgatgctg
      781 aaagacggtc aagtaacagc tactgctact tacgatcaat atttgaccgc actcagtctc
      841 atcgaacaga tccggaatga aaaaataaga gccgcatacg cgtcaaaagc agaaacagtc
      901 gctcagcaaa tgggctatag caatacgagc tattaataag cgagaattca agcaaggcta
      961 gcaaacggag gaaactatgg caaaacgaag aagaagatca agagggaaaa agatatttct
     1021 aattatttta gcaattcttg tcgtattagt attagcaatc gtaggagtag gagcaaaatt
     1081 atatctagat ctctcacatt ctattgaaaa aacttacgaa tcagtggaac gaacccaagg
     1141 agaacgaaca aaaccagttg atctggacaa acaagaatct ttctctgttc tattgatggg
     1201 gattgatacg ggagatttag gtcgggtcga tcaaggaaga tcagatacga tgatggttgc
     1261 cacagtcagt cctgaagaca aacagacaac gattgtcagc attcctcgtg atacgtatgt
     1321 cgacatcgtt ggacatgaca caaaagacaa gatcaaccac gcttacgcat tcggcggagc
     1381 tgccatggcg atggataccg tacaaaaata tgtagacatc ccaatcgatc actatgtttc
     1441 aatcaatatg aaaggtctaa aagaactcgt tgatgcagtt ggtggtatcg aagtcaataa
     1501 tgacctcacc ttctcacaag atggctatga ttttacgatc ggtaaaatca cattgaacgg
     1561 ggatcaggca ttgtcttatt ctcgtatgcg ttatgaagat ccaaatggtg actacggcag
     1621 acaagaacgt caaagaaaga tcgtagaagg tgtagcgaaa aaagtgttga gtctgaacgg
     1681 tgtaagcaac tatcaatccg ttttgaatgc tctagagtcg aatatgaaaa cagacatgag
     1741 ctttgatgaa atgaaaaaga ttgctttaga ttatcgggat gcatttggta cgatcaagca
     1801 agatcagctg caaggagaag gcttcatgca agacaatatc tcataccaaa aagtcagcga
     1861 tcaagaactt gatcgtgtga aaaaagaatt aaaagatcaa atgaatctag agaataaata
     1921 ggaaagtgga gaaaaaatgg aagagacaat cagcttgaaa gaaatttttg aaacattacg
     1981 caaacacgtg ataacaatcc tcatctcaat gtttgctggc ttggccatct caggcatcgt
     2041 gacgtttttc atcttgacgc caaaatacag ctcacaagcc caattgatcg tgacactgcc
     2101 gcaatcagat actgcaaatg tcaatgatgt caatacgaac ctgcaaatga tcaatacgta
     2161 taaggatatg atcgtcagtg atctagtact aaatgaagtc aaagatcgtt tagaaacaga
     2221 agacaatgta aaaatgactg ctggacaaat caaagatgcc atctccgtca atcagtcaca
     2281 aaattcacaa atgttctcga tccaagcaac aagcacaaat gcagtgacgg ctcaacaaat
     2341 cgctaataca acgtcacaag tattccaaga gaacgccaaa gacgtgatga atgtcgataa
     2401 aatctcgatc atctcaggtg cagttgccaa tacgacacct gtttcgccaa ataataaatt
     2461 gaatctagtg attggcttag tgttaggaat gatggtaggc gtaggtctag ctttcttatt
     2521 tgaattactc gaccggacag taaaagatga aaaatatgtg acggatacat tgggcttccc
     2581 gatcttaggg actgtaccag aaatgtcagc gaaagaattg aatgctggta tccgtaaaaa
     2641 aggaccagct ccgacacaac caaaagctac aaacgataaa acagcgccat cacgcagaag
     2701 ccgctcaaga gtctaggagg aaagaaaaat ggcacgaaca cagaaacaaa aagcagccca
     2761 aactcatgca gtcagtttgg tcacactcac aaacccctct tcaccaatcg ctgaacaata
     2821 tcgtacgatt cggacgaata tccagtttgc ctccgcagct ggtcagcaaa ttaaaacgat
     2881 tgttgtgact tcatcaggac ctggagaagg gaaatcgacc actgcagcaa acattgccgt
     2941 tgtttttgcc aaatctggtc aacgggtgct gctagtcgat gcagacttac gtaagcccgt
     3001 catctataaa acgttcaagt tgaacaacgc aagcggatta agtaccgttc ttagttcatc
     3061 aggaagtgta gcagatgaga tccaacgaac accagtcgag aatttgtcga ttctgccaag
     3121 tggacctaaa ccaccaaatc catcagaact actaagttcg ccaagaatgg atcagatttt
     3181 agcagaagca agacaactat ttgatgtagt gatcttcgac atgccgccag tagttgccgt
     3241 aacagatgca cagatcatgt cttccaaaac ggatggcacc ttattggtcg tacgtgaaaa
     3301 tatgtcacgg aaagaatcat tgactaaagc aaaagaattg ctggatatgg tacaagcgcg
     3361 agtccttggt gttgtataca atggggcaga acacagcaaa gatgctggct attactacgg
     3421 gaattaagaa ataaaaagag aggagacaag agaaatgatc gatctacatt gccatatcct
     3481 gccaggaatc gatgacggag cagaaaacat agatgccagt atcgccatgg ctgaaaaagc
     3541 gattcaacaa gggatcacac atattttatg cacaccccat cataataatg gaaaatacag
     3601 caatgaaaaa tcacaagtca tttctctagt cgcttcgtta caagcagaat tagagaaaag
     3661 acaattgcct ttgacgttat tggaaggaca agaagtacgt attaccggaa cactgatcga
     3721 agacatcgac cgagatgaaa ttttatttac agatttagat gatacatatc ttctaatcga
     3781 atttccgaca ctggaagtgc cgttatatgc agaacagctc ttttttgagt tgttacaact
     3841 aggaaagaca ccagtgatcg tccatccaga acgcaacgcg cattttcgta aagacccgaa
     3901 tcatatgctg ccattcctag atatgggatg cttggcccaa ctcaccgcac caagttatgt
     3961 cggtgtattt ggaaaagaca tccagaaaac agccaaaatg atggtcaaac ataatcttgt
     4021 gcagatggct gcttctgatg cccatggggt ctctaagcgc accttttact taaaagagtg
     4081 ctataagcag attgccaagg actttggtaa ggaaaaagta gtacagatga aacaagtcgc
     4141 aaaggatctg atcaacgggg atgaaatcag ttatccagtc tatgaagaga taaaaaagaa
     4201 gaagtttggt ttattttagg gggagtgtaa ggtgtttgta ttaacaagaa gaagaaaaat
     4261 tgccacattg gttgttgtgg acagtttgtt attgatcgct gcaaatattg cagcagctaa
     4321 attcatgaag ccgtttgtag ctataccaat ggaccttatt ttaatctcta ccggtttgtc
     4381 gattggtttt tatttatttt atggtagttt attcaaagtt ttcactcgga tcaatcgcta
     4441 tacaaattta agagagatca ttgcaatttt tggttcacta tctgcaagtg acgtctcaag
     4501 tatcctcata ctgctgttca ttaatcggca atacagttta cggttagtta tctttgctta
     4561 tctgctttcg ctcttattga tcatcggtag ccgattgatc tggcgtatct acatcgaaac
     4621 aaaaaatatg agatatgtat cagcggattc tgctaaaaat acattgatcg ttggtgctgg
     4681 cgaaggcggg cgtattttat acaacagctt tctgggatcc aaaacggctc aagatatcca
     4741 agttgtcgga tttgtagatg atgatccgaa taaaagaaat acgtatcttt caggaaaaaa
     4801 agtattaggt gcattaaaag atattccgga attgattgaa aaatatgaca ttcaaatggt
     4861 aacgatcgct atcccttcct tatcaagaaa aaaattacga agaatcttcg agttagtcga
     4921 atccgctcac gtgaaagtca acacgatgcc ttctatagaa gaacttgctt caggaaaaat
     4981 tagcgtatca aaattaaaaa caattgacgt agtggattta ctgggaagag atgaggtaaa
     5041 gctggatatt gaatccatca aagatcagat cacagacaaa gtcattcttg tcaccggcgc
     5101 aggaggatcg atcggttcag aaatctgtcg gcaaatcatt caattcaatc ctgcaaagct
     5161 attgctttta ggacatgggg agaactcgat ctacttgatt gaccgtgagt tacgcacgca
     5221 tcatcaaaat tgtcctacag aaatcgtacc gatcatcgca gatatccaag atcgcgagaa
     5281 aatcaatgag atcatggaac aatatcatcc agatatcgtc taccatgcag ctgctcataa
     5341 acatgtacca ctgatggaat ataatccaaa agaagcagtc aaaaacaata ttttcggtac
     5401 aaaaaatgta gcagaagcag ctaaagcagc aaaagtgaaa aacttcgtca tggtatcgac
     5461 agataaagct aacaatcctc caaatgtcat gggttcaaca aaacgaatcg ctgaaatgat
     5521 cgtgactgga ttgaatgaag aaggctgtac gaaattctca gctgtcagat ttgggaatgt
     5581 ccttggttct cgcggtagtg tgattccagt attcagagaa caaattgcta aaggtggtcc
     5641 aatcactgta acagactttc gtatgacccg atacttcatg acgattcctg aagctagtcg
     5701 tttagttatt caatctggcg cattagcaaa aggtggcgaa atttttattc tagatatgag
     5761 tgaaccagtg aagattgtag acctagccaa aaacatgatc cgattgagcg ggtatagcga
     5821 agacgaaatc gaaatcattg aaacaggtat ccgtccaggg gaaaaactat atgaagaact
     5881 actgttggat aaggaaagaa atgatgaagc agtttatgag aaaatctttg taggaaacat
     5941 caaaggttac tcaatacaaa aagtgatgga ttttgtcaag agcctgccgc aagacgatga
     6001 acaattggct aaagatatcg tcacttttgc aaatgcttcg aataaatagg ggggaaatat
     6061 aaatgaataa attagaaaaa cgtcagatac cattttcacc gccagatatt actgatttag
     6121 aaataaaaaa cgttgtagaa gtattaaaat ctggatggat aactacggga ccaaagacaa
     6181 aaaaattaga aaaaaaaatt acaaagtggt taggaacttc taaagtagtt tgtttaaatt
     6241 cacaaactgc tgctgcagaa acagggttac gtttgctggg aataggagaa aatgatgagg
     6301 ttatcgtccc agcttatact tattctgcca gtgcatcggt tgtatgccac ataggcgcga
     6361 acctagtatt aatagactgt caacaagatt ctttggagat ggattacgat gcattagaag
     6421 cagcaattac ggagaagaca aaagtgatta ttcctgttga tcttggtggt ataccttgtg
     6481 actacgaccg tatttttgag attgtggaga aaaaaagaaa tttatttcaa cctagtaatg
     6541 agattcagaa agcttatggt cgtataattg ttatggcaga ttctgctcat gcatttggag
     6601 ctaattggca tgatcagatg gttggtaatg ttgctgactt gacaacattt tcatttcatg
     6661 ctgttaagaa ttttacaact gcagaaggtg gagctttagt atggaaagag cacgacaaac
     6721 tagataatga ggcaatgtat catcagtgcc agctgctttc tttgcatgga cagtcaaaag
     6781 atgcacttac taaaacacag ttgggtgcat gggaatatga tattgtgggt ccatggtata
     6841 aatgtaatat gactgatgtt actgcatcaa ttgggttggc acaatttgaa cgctatccta
     6901 atattctgaa aagaagaaag aatataatta agaggtatga tgaagccttt caacaactag
     6961 gaattgaagt tcttatgcat tatacggatg attatagttc ttcaggccat ttatatatta
     7021 ctagagtacc taacattagt tctgagcaaa gaaatgaaat aattataaaa atggcagaac
     7081 aagggattgc ttgcaacgtt cactataaac cacttccaat gtttacagca tataaaaata
     7141 tggggtttga tattaataac tatcctaatg cctatgctca ttttgtaaat gagattacat
     7201 taccattgca cacgaaactt acagatgaag aagtagaata cgtaattaat aactatagta
     7261 agattgtaaa ggatttggtt ggatgatagg agaggcaaaa tgagaaagtg ggaagatttg
     7321 ccaaaagaaa tgcagaatga tgaaatacgg tattattatg atatcctcag taaaaaaaaa
     7381 ttcagcttag tggtaaaaag agtatttgat attttagtgt ctgcaacttt gttactgaca
     7441 ctttcatggt tattttttat tcttgcaata gtgataaaaa ttgattctcc tggacctgta
     7501 ttttatagac aagaacgtat tactcgttat ggaaaaaaat ttaaaattta taagtttcgt
     7561 actatggtaa taaatgcaga taaagatggc actcttgtta ctgtgaaaaa tgatgatcgt
     7621 attacaagag taggaaaggt aatccgtaaa tatagattag atgaaattag tcagctaatt
     7681 aacgttttgg tcggagatat gtcttttgta ggaaccagac cagaagttgg aaaatatgta
     7741 aagtcgtatt caccattaat gttagccaca ttattattac cagccgggat tacatcagaa
     7801 actagtattt tatacaaaga tgaagataaa ttgttgcatg ataaaaataa catcgacaaa
     7861 acttatatag aaaaaattct tcccagtaaa atgtattaca atttgaaaag tatcagtgaa
     7921 tttagttgta tgaatgattt gaaaataatg tgtaaaacta tttttgttat atttggtaaa
     7981 gaatatcacg ccgacttaga aggtgaacga gagtttatta atcagataaa atgaatggag
     8041 gataagagtt ttggtgattg aagagataaa aagttcggat aaagagttaa ttaattcgat
     8101 tgtcgatatt catatgagaa catttacaaa tttttttttg actttcatgg gaaaaaagtt
     8161 tttggaattg atgtatcgtt cttacacaga atataatgat tctggtatat tggttgctat
     8221 tcaaaatgat aagccaatag gatttcttgc atattctgga gatatgagtg gtttatataa
     8281 gtatatgata aagaaaagaa taataccgtt tggattttat agttgtgtag cctttataaa
     8341 gaaacccaat atccttatga ggctttttag agcttttcta aaaccaagcg aaacaaaacg
     8401 tgatgaaaat tatgtggaat tagcatccat tggagttaat ccgaaagaga aatcgatagg
     8461 agtaggttcc atgcttatag ataacttaaa gagtagaatt gatttttcaa aatatgaata
     8521 tattgcacta gaaacagatg cattaaataa tgatgttgtg aataattttt ataaaaagaa
     8581 tgagttcatt ttagaacgta aatattctac taatgaagga agaattatga atgaatatcg
     8641 gtt
//
LOCUS       DACCXO010000117         8501 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.120, whole genome shotgun sequence.
ACCESSION   DACCXO010000117 DACCXO010000000
VERSION     DACCXO010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.120"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            179..328
                     /locus_tag="IVY70_002421"
     CDS             179..328
                     /locus_tag="IVY70_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP7959530.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            339..1853
                     /gene="dltA"
                     /locus_tag="IVY70_002422"
     CDS             339..1853
                     /gene="dltA"
                     /locus_tag="IVY70_002422"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP7959531.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1850..3055
                     /gene="dltB"
                     /locus_tag="IVY70_002423"
     CDS             1850..3055
                     /gene="dltB"
                     /locus_tag="IVY70_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP7959532.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3100..3333
                     /gene="dltC"
                     /locus_tag="IVY70_002424"
     CDS             3100..3333
                     /gene="dltC"
                     /locus_tag="IVY70_002424"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP7959533.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3336..4604
                     /gene="dltD"
                     /locus_tag="IVY70_002425"
     CDS             3336..4604
                     /gene="dltD"
                     /locus_tag="IVY70_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP7959534.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4745..5827
                     /locus_tag="IVY70_002426"
     CDS             4745..5827
                     /locus_tag="IVY70_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP7959535.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5827..6630
                     /locus_tag="IVY70_002427"
     CDS             5827..6630
                     /locus_tag="IVY70_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP7959536.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6700..7440
                     /locus_tag="IVY70_002428"
     CDS             6700..7440
                     /locus_tag="IVY70_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7959537.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7451..8455
                     /locus_tag="IVY70_002429"
     CDS             7451..8455
                     /locus_tag="IVY70_002429"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP7959538.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
BASE COUNT         2736 a         1397 c         1748 g         2620 t
ORIGIN      
        1 tatatttttt aaaagtacga gaaattataa agaaaaaagc agtgatttga tgtgctattt
       61 ttataaaagc tatgtgacaa aactgttagt aaaaaaacgg tttctgtaag ctagacacat
      121 gtttcttttt gtcaaaatta gttacacttt gtgtgaggtt atatgaggag ggaatccaat
      181 gaacaaatgg cgttctaatc caaaattgaa ttattgggga aaattcatca ttcgaacaat
      241 tttttatagt gcagtactgc tgttcctgat ttatctgtat cactataaaa atattcaagg
      301 aggaactttt atctataacg aattttagga ggaagagtat ggaaatcaaa acgattattg
      361 aagcaatcga tgattgggga ataaatgaac ctgatcgagt tgcttatcag gcggatgaga
      421 cgcatacttt tggtgaattg aaacaagcat cagacgcact tgcttattat ttagaaaaaa
      481 aggtagaagg cgatggacca attgtcgttt ttggcaattt ggagtttgaa atgattgtct
      541 catttctagg ggttgtcaaa tctggccatg cctatttgcc gattgaagag catacaccca
      601 aggagcgtat cctatcgatc tttcgggtgg ctaaaccatc tatggtcatc agtatcgggg
      661 actggataga agatattcct tcggttcctg tcataacaaa aaaagaattt caagaaatca
      721 agaagatacc agtaggattt tatcccagaa atagcgtaaa aggtacagaa aatttttata
      781 ttatttttac ttctggtact actggagaac caaaaggcgt acaaatcagt catgataatc
      841 tagtcagctt tgttcaatgg acgatggaag actttgggat caagcctggt atgcatttct
      901 tagcccaagc gccattttct tttgatttat cggtattttc tatttatcct gcattagttt
      961 ctggcggaat gctgaagccg ttaaataaag cagttgttca agactttcgt caattattcg
     1021 cgacattgcc agccttgaag ttgaatgtgt gggtttctac tccatcattt atggatattt
     1081 gcttgatgga acctacattc aatggagaaa atgtgccgga attggacatg tttttatttt
     1141 gcggagaaga attgacgaaa aaaactgcag aaagtctact tgatcgattc ccaaatgcac
     1201 ggatatataa tacttacggt ccaacagaag caactgtagc tatttcaagt attcagattg
     1261 accagcatgt actagatagt tatgaccgac taccgattgg ctacgtaaaa aaagacacga
     1321 aagtttctat tgtccaagaa ggtaaacctg tttcaaaagg agaaacgggt gaaatcatta
     1381 ttgcaggacc aagcgtatca aaaggttacc tgaacaatcc cgaaaagaca gctgctgcgt
     1441 tcttttgtca aaatgactta gcaagttacc atacagggga tgcaggtaga ctagatgaat
     1501 atgggctttt attttatgaa ggacgtatgg atcagcaggt caaattacat ggataccgga
     1561 ttgaactcgg ggatatcgaa cattatctgc tccaagacaa ccgaatcaaa caagctgtcg
     1621 tcgtaccaaa ataccaagga acaaaagtac agcagctggt tgcttttgtc gtattagaaa
     1681 acaaaacgaa acagccggat tttcagttga ctaaatctat caaagaacaa ttactagaag
     1741 ttgtaatgga ttatatgatt cctcagaagt tccattatgt cgatctgctg cctcagacag
     1801 ttaatggaaa aatcgatcga aaaaaattaa cagctgaggt gaatcctgaa tgatgttacc
     1861 tcatatgatc ccttatgcag agccgtttta tttttttctt ttagccattg cgcttgtacc
     1921 gatcgttcta tctttattga tatgggaaaa aagattacca gtttatcaat cagcagtgac
     1981 acttttcttt ttatttgtca gctttggagg taattattgg caacagggag cggcgctgat
     2041 tgggtatgtt ttgtggcaga cgatacttgt atggggctat tttgtttatc ggcaaaggaa
     2101 aaatcagaca ggatggtttt ttgccgccgt tattttagcg ataattccac tgtttttaac
     2161 gaaagtgact cctttttttg aacatggaaa agcatcgtta cttggttttt taggaatctc
     2221 ttatttaacc tttaaatctg tacaaatgat catggaaata agagatggca tgatcaaaac
     2281 atataagcca tttcgttata tccagtttct gctgttcttt ccaacaatct cttctggacc
     2341 aatcgatcgc tataggagat ttgaaaaaga tcagctccat ccgcctgttc gtgataaata
     2401 tgttgatctt ttgggaaaag gcgtccaaaa tatttttgtc ggttttttat ataaattcat
     2461 tatcggttat tattttggtc aagtactgct tcctgtagtg ggacgcattg ccttacagca
     2521 tggtggtatt tcatgggcat tagtaggtta tatgtacgtg tacagtatgt atttgttttt
     2581 tgattttgct ggctatagct tatttgcagt cggtacaagc tacatgatgg gatatcagac
     2641 accaatcaat ttcaataaac cttttttatc atggaatatc aaagaattct ggaatcgttg
     2701 gcatatgacg ctgtcctttt ggttccgtga ttatgtatat atgcgattga tgttcttcat
     2761 gatgaagaaa aaatttttta aaagcaagat cgtaatgtca aatatcggtt attttgcttt
     2821 gtttcttcta atgggtatat ggcatggatt gacgtggtac tacatcgtat atggactgta
     2881 tcatgcttgt ctgatctgtc tgacagatgc atggatccga tttaagaaga agcataaaga
     2941 gcagttgcct tctaacaaat ttacgcatgg cttagccgta tttttgacat ttcaagcagt
     3001 atgttttagc ttcctgattt tttcaggatt tttagataaa ctttttttta gctaacgaat
     3061 agtgacatat taatttatat aacaaaggag aaaaggaaaa tggaagaaca aattttatca
     3121 atcttagaag aaataacagg aacagatgaa gtaagaaaaa atcgtgatat tgatctattt
     3181 gatgaagggt tgcttgattc tttagcatct gttcaattat tagtcgagct agacggagag
     3241 ctggatatcc aagtccctgt atctgaattc gagcgagagg attggagcac accgaataaa
     3301 atcattcaac aagcaactgc attgaagggt tgagtatgaa aaagaaactt tttgctattt
     3361 tcggcccggt tgttttagct gttggcttac tgtttgtgtt ttttacctcc tcctatcaag
     3421 tagatatctc taatccagaa acgattagac gtgcctcttc ttccatgtca gtgaacgtgt
     3481 tgaaaggaga tgcgataaaa aattatgcat tatctgaaaa acaatatatt cctttttttg
     3541 gctcaagtga attaagcaga atcagtccat ttcatccgtc tgttctggca gaaaaatacc
     3601 agcgaaacta tcgtccattt ttgctgggag cacccgggac acaatcgtta agccaatata
     3661 tgatgatgcg ttctgcagga gatgctatga aaaacaagaa agtcgtcttt attatttctc
     3721 ctcagtggtt cgttaaaaat ggtgtaaaaa ctgattactt taatacgtat tattctgaac
     3781 ttcaaacata cgactggttg ttttcaatga agaaagtaac gcctgctgat cgctatttag
     3841 ctagaagatt attaactttc tcaaaagtaa aagagaatga tacgttgacg gcgattctgc
     3901 aaacgatcaa aaaagggaag ctacctttac ctgaatcttt gaatcagctg cgcagccaat
     3961 ggaacatgct gaagagagag gatgaagtat tcagcaatat tgggttgaaa gatcgacagc
     4021 aaaaaatcga tcatgagtca aaacgattac caaaacaata ccaagaaaca gaattatcca
     4081 ttttagcaaa tcaaattgga gaacgagaaa cgacgaataa tccattcggg ttgaaaaacg
     4141 atttttatac gcatcgtatc cgagcccacg agccagagct caaacagtca caaaaaaatt
     4201 gggattatcg tttttcgcca gaattttctg attttcagct ggtcttggat caattagcaa
     4261 aaaaccataa cgaagtattg ttcatcatcc caccggttaa cgagaaatgg agcgattata
     4321 ccggactatc tcaagaaatg ttacaaggat ttgccaaaaa gatcaaattt caattaaata
     4381 gccaaggatt caatcgaata gctgattttg tcaatcaagc gggtaccaac tattttatgg
     4441 aagacacgat ccatctaggc tggaaaggct ggttggctgc agatcaacag atcagaccgt
     4501 ttttagaaga aaatcatatt actgctagta agtatcatct tgacgatgct ttttttagca
     4561 aatcttggca acatcaaata ccagataaac tacaattaaa ataaagaaaa caggcgagtg
     4621 ttgctcgctt gttttcttta tttttgaata ttctgaataa aagataaaaa taccaagttt
     4681 gcctgaacaa aaatcataag caatggtata atcacaatat gaatatagaa atgggggaaa
     4741 acatttggat acaaaagaag aacagctact tattgattta acttctgcaa aaggagtacc
     4801 tggaaatgaa gaagaggtaa gagaagtatt cagagaatat gccaaaccat tcgctgatga
     4861 tattttttat gacggtttag gcagtgtgat tgccaaacat ggtgctggcg gaggccccaa
     4921 ggtctttatt tctggacata tggacgaagt tggttttatg gtaacaaaaa taaccgaaaa
     4981 aggattcttg gaattccaaa ctcttggagg ttggtggggg caagtcatgc ttgctcagca
     5041 agttgaaatc aaaacacgtg aagggaaaat cgtccacggt gtgattggtt ccaaaccacc
     5101 acatgtttta acacctcaag taagaaataa accttatgaa ataaaggata tgttcattga
     5161 tattggcgca tcaagccaag aagaagccaa agagtggggc atacgtcctg gagatatggt
     5221 cactccatat attgaataca aacgaatgaa tggcagtaaa tatttactgg caaaagcatg
     5281 ggataatcgt atcgggacag ctgtttctct gcgagtatta gagaatctat ctaaagaagc
     5341 acacccaaat gttttattcg caggaagcga tgtgcaagaa gaagtaggct tgcgaggtgc
     5401 tcgaacaagt acacaccttg taaatcccga tattgctttt gctttagata ctggtacagc
     5461 aggagacact ccaggaatga caccaaaaga agcagattcg attttaggaa aaggcccaca
     5521 gattttgatt tttgatgcat ctatgatccc gcataaaaaa ttattgaatt ttgttattga
     5581 tgtagcagag gaaatggata ttccattcca atatacagtg atttctggcg gaggaactga
     5641 tgcaggacaa atgcatttga caagagatgg tgttccgtct gtagccatta ccgttcctgt
     5701 tcgttatctg cattcccata catcagtgat tcatgaagat gattatttga atacagtgaa
     5761 actagtcaca gaattagtga aacggcttga tagtgaaaca gttgaaaaaa ttcggagtta
     5821 tgaataatga ataatagaat acctatttct gaatatcgga aaaaagcacg agtttctttg
     5881 gaaaatcaat ggggaatcaa tacttggctt gttttcttat ctataatatt tacgagcgtc
     5941 atccagaact tttttgacgg tttcagtcct tttccggaag aaagcatgca gcagcactta
     6001 ttgagttttc tagtcgataa tttactttta ttcgcattca actatgcatt gttttacatt
     6061 gcgctagtag ttgtacgggg tggcagacca aaaagtaacc ttttattttc agttttccaa
     6121 agaaagtatt ttggttcgat acttggcatc aatttgatca attcagtcat caactggcta
     6181 ctcaacagtt taatctttct gcccatgttt ttaatcagcg gagtgagtac ttatatgcag
     6241 ctgcttttca gttcaaatgc ggtacctacg ggtgtcatgc cagaagattt tcttgatatc
     6301 agctttgttt tatcgatgat agggatgatt tttgtttcag cactcatcat gagtattgtt
     6361 ggcggtttat tccaatttgc tgcatggaca aagttcgact atcctgattt gtcggttatt
     6421 caatgtttga aatatggctg gtttttattg aaagatagaa ttggtacgta tattttgttg
     6481 cagctgtctt ttatcggctg gtatttgcta ggagcaatcg ctttgttcat cggtcttttg
     6541 tgggtcattg tttatgtgaa tgtgtcagtt gcagaatttt atgaacaagc acggattgaa
     6601 aaaggaggtc cagacgccta ttttacttag catgtaagca gtttattcat caaaaaaata
     6661 acttttgtgg taggataaat ctttgagggg gaatgtacga tgaaattaac tgttttagga
     6721 tgcttaggag cgtatcctta taaaaatcaa gggacaacag gttatttgct tcaatctgac
     6781 gggttcaatt tgttgatcga tgcaggtagt gctacgctga tcaaattaca agaacacctt
     6841 gatccacttg atctagatgc tgtgatcctt agccattatc atcatgatca tattgctgac
     6901 cttggtgtat tacagtatta ctggcaactt catcctaaga gaaatccgga gacattgctt
     6961 tcaatttatg gacacacaaa agattccatt cactttgaag aattgacgtt ggaaaatgtc
     7021 tcaaaaggac atccttattt tgaagctgaa gaattaaaaa ttggtccttt tctgattact
     7081 tttatggaaa caatccatcc agtagtatgt tacgccatgc gtatcgtaga agaaacaacg
     7141 gggaaagtat tcgtatttac aggtgattca ggttatttgg aaagtttcgc tgattttgcg
     7201 aaagatgctg atttgtttct agcagatacc tatctttttg aaggaaatga acatcatcat
     7261 gcccatttca cttcaaaaga agctggagaa cttgcgaaat cagctaacgt aaagaaactt
     7321 gtacttacac acctacctca gtttggtgat ttagaacaat tgaaaaatga agcgcaaaag
     7381 gcagcaggca aagggattga tatagaactt gctgcagttg acaaagtgtt taacatttag
     7441 gaggaagaac atgatttttg taccaaatga aaaccacgac ccaagaatca atttagcaat
     7501 tgaacaatat ttgctgcaag aaatgccttt agatgaacct attttgctct tttatatcaa
     7561 tgaaccttca atcatcatcg gcagaaacca aaatacgctt gaagaaatca atcgagatta
     7621 tgtggaagaa aatggcattc atgttgtccg tcgcttaagt ggaggcggag ctgtctacca
     7681 tgattttgga aacctgaact ttagttttat catgcctgat gatggggatt cattccgtga
     7741 ctttgcgaaa gtaacacagc caatcatcca agcacttcat gaattgggtg ttgctggggc
     7801 ggaattgaaa ggccgaaatg atctagtcat tgatggcatg aaattttctg gcaacgcgat
     7861 gtatgctacc aacggtcgga tgtttgctca tggaacaatt atgttcgact ctgatatcaa
     7921 tgaagtagtc aatgcattaa aagtaaaaaa agacaaaatc gaatcaaaag gaatcaaatc
     7981 gatccgttct cgtgtaacga atatcaaacc atttcttcca gaagaaaagc aaaaaatgac
     8041 aacagaagaa tttcgtcaag aaatcttgtt gaaaattttc ggcgtagagt cagtagaaga
     8101 agtaaaaacg tacgaattga ctgatgaaga ttggaaaaaa atcaatcgaa tctctgagga
     8161 atattatcga aattgggatt ggaattacgg gaagtctcca gatttcaatt tctctcgcca
     8221 aaaacgtttt tctatcggtt cgattgaagt ccatttgaat gtctcagaag gaatgatcaa
     8281 agaagcaaaa atctttggcg atttcttcgg actaggagat atctctgacg tggaaaaaaa
     8341 attggttgga cagaaatatg ataaagcatc tctatcaaaa atagtcagcc aaattgatgt
     8401 gaagaaatat tttggtgcga tagaaccaga agaattgctt accttacttt attgaaaata
     8461 aaaaatagcg gaatccacat atatggattc cgctattttt t
//
LOCUS       DACCXO010000118         8405 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.121, whole genome shotgun sequence.
ACCESSION   DACCXO010000118 DACCXO010000000
VERSION     DACCXO010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.121"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            130..1557
                     /locus_tag="IVY70_002430"
     CDS             130..1557
                     /locus_tag="IVY70_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7959539.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1649..2014)
                     /locus_tag="IVY70_002431"
     CDS             complement(1649..2014)
                     /locus_tag="IVY70_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP7959540.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(2011..2376)
                     /locus_tag="IVY70_002432"
     CDS             complement(2011..2376)
                     /locus_tag="IVY70_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP7959541.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2568..2816)
                     /locus_tag="IVY70_002433"
     CDS             complement(2568..2816)
                     /locus_tag="IVY70_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959542.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2815..2943
                     /locus_tag="IVY70_002434"
     CDS             2815..2943
                     /locus_tag="IVY70_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP7959543.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            3000..4694
                     /locus_tag="IVY70_002435"
     CDS             3000..4694
                     /locus_tag="IVY70_002435"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP7959544.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4761..4967
                     /locus_tag="IVY70_002436"
     CDS             4761..4967
                     /locus_tag="IVY70_002436"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959545.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5345..6478
                     /locus_tag="IVY70_002437"
     CDS             5345..6478
                     /locus_tag="IVY70_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP7959546.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6509..6829
                     /locus_tag="IVY70_002438"
     CDS             6509..6829
                     /locus_tag="IVY70_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959547.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6858..7322)
                     /locus_tag="IVY70_002439"
     CDS             complement(6858..7322)
                     /locus_tag="IVY70_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7959548.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7454..>8405)
                     /locus_tag="IVY70_002440"
     CDS             complement(7454..>8405)
                     /locus_tag="IVY70_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7959549.1"
                     /translation="KDGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVR
                     LSGNEKLIIKMPDGVPPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTV
                     VQEPFMGLANSYPSALLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAG
                     HSAPFIVNKPFFDSFVNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINE
                     PTMFGLPIVLNPIMFIPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLA
                     TKSIAGGVLAAVNLVLSILIYLPFVKVATIQERRKEALDA"
BASE COUNT         2763 a         1625 c         1520 g         2497 t
ORIGIN      
        1 ttataacata aaaaaccttt tttaacggca tttatatgct ttttcagaaa ttaactgatg
       61 atttcaaaaa gaaaacatgt cataatattt cccatattgt gcaaaaaaat acaaggcgga
      121 ggctctacta tgattgaaga atatattgag aaaaatattt taagacagct gtttttatgt
      181 gggcaattct atgtaaataa agaagtcaat ctagaaaagc tctccaatct cctgcatgtt
      241 tgcaaaacca ctttactcaa tgatatcaat aacataaaaa aagaatttga agaacagatc
      301 gcttatacac atcgggaaaa ggaccgttac actttatatt tttcagaaca tatacctcga
      361 tgcaagatca tgcagcaatt gtctcaaaat tcgttgtttt taaaaacttg tctgctttat
      421 ttggaagaag atgaacctga ttatcttcaa ttaactgaat gtgaattcat ctctgtcagc
      481 aaagcttatt cattaaaaaa acaagtatta gcttatttta atgactgcgg gatcgaaatc
      541 gaccgctact cccctcgatt cactgaaatg gaaagacgtc tgctgcttct taatgtgtcg
      601 tatcgtttag gcggttttaa ttcttgggag ctacctgaat cgttttttga acgtgccgat
      661 cgtttcattg aatccgtaac ggaaaatagt ggcagatttt atgataaaga aaataaagaa
      721 atcttatcga ttggttttgc tatcagtttc ctacgccagc aagtgtgtgc tgtgacaata
      781 gattcaaaat ttatcgaaga aatcaaaaaa cgtcctattt acaattatgt cgaatcagca
      841 tgggaaaaca ctgatttcca aacctattat aaaaaagaag aattcgcctt tattttgaca
      901 ctattcaatc tttgtaatta tggtttccat tcttatcagt tgattgcgga agactttcaa
      961 cagctacacc aagtgtttat tgacaatact cccgaaataa aagaactagt tgctaccttt
     1021 gaatctcact tcaatcaaga attattcgga aaccaaccgt tcgaacgtgc gctgattcat
     1081 ctgatgcgtt cggcttggga taactatcaa ttattcatgc cagaaaaatt ttatctttta
     1141 aatgaggaac agacaaattt atataaagaa gtccagacaa ttttttccag ctggtccagc
     1201 cagttacctt atgatctacg gttgaatcct aattgcatgc gggcttttgt gatcgaattg
     1261 agcggaattc tccgtctgac aaaagaacat ctgactatct atatcgtcac aaattcggat
     1321 gttcattatc tgatttatcg ggaagccttg gaagctgtaa ctactttcga ttttcaagtt
     1381 gcaccaacaa tttattcaag tatatctgat ataaagaagt atgcgcagca gtcctcaaac
     1441 cgggtattat gtgaacgtac attatataca ccagatgcgg tccaatatga aaatattatt
     1501 cctatttcga tcaatacgat cgatcgagct atcatttcag ctgtgcaaaa caaataaggg
     1561 gctgtgacaa aaatcatgtc acagcctctt atttgttgaa gatgcccatt tctgtaacaa
     1621 tctcttgata cacgtcaggc tcacaacctc acttattttg gacatctacg ggtccgatct
     1681 ctttatttaa aaaataagaa atgatggttc ggataactac caacgccgct agttggaaaa
     1741 tatcctgcaa ggtaggtttg ataatcgatt cgatgatccc agctgaaaca agaatttcca
     1801 aacttagaag tatatagcct cccaacatct ttttaatagc attgttctcg ctgactcggt
     1861 cgctttgcac ttttgtatgg acaaataaat atttcaaaaa atccttgatt gatagacaaa
     1921 caccccaaac caataaacag atagcaaaga tatcaagtcc cagtacaagt ttttcgaaat
     1981 atggaagaag aaactgcatc aactctgtcg tcatcctacc tctcctcttc tttgtttttc
     2041 tttgtcttct atttcctgat tcaagaaata agaaatgact gttcgaatga taataataag
     2101 tgcaagcttc aaaatatctg tccaagtcgg cttgataact gactcgatga tatcagcaac
     2161 gactagtact tccaagctta acaaaatata acttgctagc agttttcttg tatctgcatt
     2221 acgagtatta cgttctgcat agtttttctt gacatgagac caatcaaaaa gtttgaatag
     2281 aacaagaacg atcccgacaa ttaagatacc gatagaacaa atatttaaaa tagcaatgat
     2341 ccattctaga aattcaatta aatttgagcg aaacatatac gctacctact ttcttttaat
     2401 ttgttcctat tcttttattg taaaagaaag ttaccttgtt cgtctattta aaagttgttc
     2461 taatgataac ggttcgctta aatttttctt tttcactctc ccatttttct ccctccatcc
     2521 ataaatcgtg gaagtatcca aaacaacgta gcaagttaaa ataaggctta gtccgtcatc
     2581 agcttttcaa agaagcactg tctcggtgct ttttttgtac ccaaatttct aaaaatagaa
     2641 ccatacacct cctcaaatct tttcttctta cctaccctat ttttatttac aggaaaatca
     2701 gacacttctt cttttttgtc aaaatttcct ttttcttctc gtcctatcat ttggcattct
     2761 aataaaaacg aactatcctt ggatatagaa agagaaaaca aggaggcgtt tttcatgagt
     2821 aaaaaattaa tcaaagtcgg catcggttta ggtttgcttg cccttggcgc tgcatatcta
     2881 ggaaaaaaga caggactatt cgaagatgac agtcatttat atgatgaatt cgaatcaatc
     2941 tagcatccta tttagttgaa tcactacgga catacttgga agacattgga ggaatcacaa
     3001 tgagaaaaag taaagcaata atgattggtg ccggtattgc caatatggca gcggctgttt
     3061 atttgattca agaaggcaaa tggaaagggg atcagatcac cttttattca ttggatgatc
     3121 acggttcaaa tgatggtgca ccgaccgaca ctgtagtgga tgaatattgg aataaaaacc
     3181 atccattaga aaaccaaaaa ggctatattg ctcgaggcgg tcgcatgctt aattaccgta
     3241 cctatgtaga tttgatggat ctgttaagtc gaataccttc tgttactgag ccagggatga
     3301 ctgctgaaga agatacgaga gattttgatg caaaacaccg aacctttgac aaagcacgtt
     3361 tgatggaagg tggcaaagga atcattgatg gcggaaaact gggattcaat aataaagacc
     3421 gtattcttct gactaaactg attgctatgc ctgattcaga agaagaaaaa ctagacaata
     3481 tcactatcga agaatatttc aaagatgatc cacacttttt tgaaacaaac ttctggttta
     3541 tgtgggaaac aacatttgct ttcagaacac gtagttctgc tcaagaattg cgtcgctata
     3601 tgcatcaaat gatttatgaa ttcactcaaa tcgaacatct agtgggcgtc aatcgaacac
     3661 gttacaatca atacgaaagt attatgcttc ctttgatcga gtacttgaaa gaacaaggat
     3721 gcaaaatcat tttgaaccgc cgtgtcactg actgggaatt caaagaaaca ccgatgcaag
     3781 atgaaatcac tgtgactggt ttgaaaatta ccaatacact gactcaagaa gaagaatacg
     3841 ttgtggtcga tgaagataca gcagttattt tcactaatgg gtccattaca gattcagcta
     3901 ctttaggaga ttacgaaaca ccagctgtag aaaatatgga ttatggtgca gcttctagtc
     3961 tctggaaaaa agccagtgaa cgtttctaca acttgggaaa tcctgacaaa ttctttgctg
     4021 atcgagatgc aagcgaatgg gtcagcttta ccctgacgac gaaaaaccat ttgttgttga
     4081 atgaaatcac ccgtatcacg actcaagaac caggaaatgc cttgaattca tttatttcaa
     4141 ccacaccgat cacgccgtta ggccaaaaag atgtcaatat gtctattgtc gtgcatcatc
     4201 aaccgcattt tacgtctcag aaaccaaatg aaacagttat ttggggttat ttcctttatc
     4261 caagacgacg tggcgaattt gtggataaac agtatattaa aatgaatgga aaagaaatgc
     4321 tagaagaact gattggacaa ctttcaaaag tagatcctgg accagtgaat attcgtgaaa
     4381 aagaaacaga aattttcgac agcgtaataa ataatatccc tgtctatatg ccttatgctt
     4441 ccgcgttgtt caataatcgt gccaaaagcg atcgaccaaa agtcattcct aaacattcga
     4501 ccaatctggc ctttacagga gaatttgtcg agcagcctta tcagatgatc tttactgaac
     4561 aaagtgctgt acgttctgga gaaatcgctg ctttccattt tgcgggtatt cctatgtccc
     4621 gtcttgtcaa aacaccgcgt tacgacaaag atattcctac tctagctcgt gccgcaaaaa
     4681 aaatgtttga ataatcaaga ggttgtgaaa aagctgccct gcatcaagta ataagaacaa
     4741 ccaggctaaa acagcttctt atgtttttga tttggttgga cttattaccg cagatgcaag
     4801 cttttgaaca ccgtttacca agaggttatg aaaaagctgc cctgcatcaa gtaataagag
     4861 cagccaagct aaaacagctt cttatgtttt tgacttggtt agacttatta ccgcagatgc
     4921 aagcttttga acaccgttta ccaagaggtt atgaaaaagg cgtttagttt cgagtatgaa
     4981 gaaaaatttt gtgaaaatgg ttcctcacgt ttttgcataa atttgactta ataccgagaa
     5041 actgatccct tctgtcataa cctcttttta tttatgtctg tttctttttg aaaacaacta
     5101 ttttttcaac gtttactctc ttgccaatcc aacatcatct aacggataca actatttcaa
     5161 tgattaccat tacactaaaa aatcggaaac acacctctcc gaacacaaat acataagact
     5221 atagaatgtt tcatttaaga aaaaaagcgt ttcattatca aaaaggtaag atctccttta
     5281 aaatgaaatt atctgtagta ctaataatgc agacaatttt atgaatctga aaggagcatc
     5341 tccaatgaaa gcagtcactt ggcaaggaaa acagaaaatg gaaatcagaa acgtagatga
     5401 tccaaaaata ttagaaccaa cagatgcaat catccgtatc acagcaacag ccatttgcgg
     5461 ctccgatctg catctttacc atcatggtga agcagttttg gaaaaagggt atgttgtagg
     5521 acacgaacca atgggaatcg ttgaagaagt gggatctcaa gttcaaacat tgaaaaaagg
     5581 gcaacgtgtg gttatccctt ttaatattag ttgtggtcat tgtcatttct gcctgaatca
     5641 catggaaagc caatgtgatg aatcaaatcc agatcagtta tatggcgggc ttttcggatt
     5701 tggcaagttg aacggtaatc attggggtgg tcaagcggaa tatctaagag ttccttatgc
     5761 agactctact tcatttgttg taccagataa tgatcttcct gatgaaaaag ttttatttct
     5821 atcagatatt ttgcccacag cttattggag cgttgaaaac gcaggagtaa aaaaaggcga
     5881 tacagttgtg attctaggtt ctggtccagt cggtctattc gctcaaaaat tcgctgttat
     5941 ggctggagca gagcgcgtaa tcgcagtaga tccagtacag caccgtctag ataaagcggc
     6001 taattacaat ggggttgaaa cttatttctt agaagacacg gcacaagctg gaacagaatt
     6061 gtatgaactg gctaaaggtg gcgcagatgt cattatcgat tgtgtaggaa tggacggatt
     6121 agagcctgtc aaagaaaaag cgaaaaattt agtaagctta caaagcggca caatctctcc
     6181 attacagatg gcatcgcaag ctgtcaaaaa atttggaaca gtccaagtaa ctggtgttta
     6241 tatgactcca gcttcttctt acccgctgca agaatttttt atgcgggata tcgctgtcaa
     6301 gcatggacag gcaccaatta tccatctaat gccaaaaatt tacgcaatga ttgcagaagg
     6361 actattcgat cctacacaaa tcattactca ttcgctgcca ctatcagaag cagcacaagc
     6421 atatgagatc tttgacaaaa aagaagataa aaatataaaa gttgttttga aaccttgaat
     6481 tcagtaaaaa atacaggtga ttttctttat gcccaaaatc aaacgaaata cccatgagat
     6541 cgaaatagga aaagaagaag atatcgaaat acgaggacgt cgcttccgct tctatttcca
     6601 aaatcgttac acattgatct cactggctgt cgatttatta accgggattt tctatatttt
     6661 tggaagcctt gcttccttaa ctaaaatccc cgatcgatat ggcacctatt tttatttggc
     6721 tggtgctatt ttcttgacga ttcgtcctat tctcagaatc gtaaggaaca tttttattta
     6781 cgacgaaaaa aaagcaatga agagaacaag caaaaaaaaa gaggcatgag aatttttcaa
     6841 atctcatgcc tcttttttca ccctatcgta tcactttgat acagacgagc ggtcgtccca
     6901 ctttcttcct tgaccattgt caaaaatgtc cactcgtatc gttcataaaa cgtggtatga
     6961 tctgtaatta agtaaagttt ttcgtaccca agttgatgca tttcttttct gatttccgct
     7021 aatatccgct tggctatccc ctgcttacgc ttttccggtt caacaaacag ggcacaaaga
     7081 tttggtgtca gatcagggcg gttatggaag tcgttttcaa tgacgcccgc tcctccgacg
     7141 atggtttctt tttcatctaa gaccacatac cattgaggaa tactttcctt attagcgatt
     7201 ccagtacgaa tactttcctc atacacaaca gcaggaactc cccattttga tgaaaaccaa
     7261 ttcgcgcttt gttttactaa ttctgggtgt tctcttaatt tgatccagtt atatgtaacc
     7321 aatgtttaaa cctccgacta aaataaaacg tttacaaaaa ttatacaagt ctttatgaaa
     7381 attagcaatg acaacaatag tgatgtaaaa aaaaaaacgg aaaacaatca cttgttctcc
     7441 gttttttgat taatcaagca tctaaagctt cttttcttct ttcttggatc gtagctacct
     7501 tgacgaacgg tagatagatc aaaatagaaa gaaccaaatt gacagcggct agtacaccac
     7561 ctgcaatact ttttgttgcc aatacaccgc caataattgg tggagttacc catggaggca
     7621 taacggtagc tgctggcact aaacctgtag aagtcgcaaa gtaagcaaca ctaactaaga
     7681 ccattggtgt caaaataaat ggaatgaaca tgatcgggtt caacacgata ggtaacccaa
     7741 acatcgttgg ttcattaata ttgaagacac ctggcgcaat cgacaagttt gttacaacca
     7801 tatatggttt gtttttacgt cctactaaat aaatagctaa cagtaaacct aaagttgcac
     7861 ctgttcctcc taagttgaca aagctgtcaa agaaaggttt gttcacgata aacggtgcgc
     7921 tatgtccagc tgcgattgca ttaacgtttg cttcaatggc tggcgcattg attgtttgca
     7981 tcaaaggatc gaccatattc gctccgtgta aaccaaagaa ccataagaat ggtgtgataa
     8041 atgcaagcag taaggcagat ggataagagt ttgcaagtcc cataaatggt tcttggacca
     8101 ctgtatagaa agaacctacg atatcatcaa caccgaatgc agcaaaaatt gcagcaacta
     8161 aagcaaacgc agagatagtg atcattgctg gtagtaatga agcaaatgct tttgcaactg
     8221 ccggtggcac accatcaggc attttgatga tcaatttttc atttccgctt aaacgtacaa
     8281 agatttctgt tgaaataatc ccaacaataa tagcaataaa caaaccgttt gagctcatac
     8341 ctaatgctcc gcctaaagcg aagaaagagc ctaaagaaat cacacctgcg gaaacaccgt
     8401 ctttt
//
LOCUS       DACCXO010000119         8231 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.122, whole genome shotgun sequence.
ACCESSION   DACCXO010000119 DACCXO010000000
VERSION     DACCXO010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.122"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            330..1253
                     /gene="truB"
                     /locus_tag="IVY70_002441"
     CDS             330..1253
                     /gene="truB"
                     /locus_tag="IVY70_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP7959550.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1257..2198
                     /gene="ribF"
                     /locus_tag="IVY70_002442"
     CDS             1257..2198
                     /gene="ribF"
                     /locus_tag="IVY70_002442"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP7959551.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2593..3378
                     /locus_tag="IVY70_002443"
     CDS             2593..3378
                     /locus_tag="IVY70_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7959552.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3391..4242
                     /locus_tag="IVY70_002444"
     CDS             3391..4242
                     /locus_tag="IVY70_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP7959553.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4366..4818)
                     /locus_tag="IVY70_002445"
     CDS             complement(4366..4818)
                     /locus_tag="IVY70_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7959554.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5283..5651)
                     /locus_tag="IVY70_002446"
     CDS             complement(5283..5651)
                     /locus_tag="IVY70_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP7959555.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5801..6283
                     /locus_tag="IVY70_002447"
     CDS             5801..6283
                     /locus_tag="IVY70_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP7959556.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6320..7330
                     /locus_tag="IVY70_002448"
     CDS             6320..7330
                     /locus_tag="IVY70_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP7959557.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7445..8158)
                     /locus_tag="IVY70_002449"
     CDS             complement(7445..8158)
                     /locus_tag="IVY70_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959558.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2785 a         1337 c         1668 g         2441 t
ORIGIN      
        1 gattaagaaa ctgaaaaaat aaataaaaaa aaacggaaaa aagactggaa gaatggacag
       61 tctttttttt cgtttttggg aagtgaaaac caaactattt acctgtaagc cacccataaa
      121 tccaatcaaa tggtgcaaag aaaaaatctt ccattctatt ccctccttta aaaaaaacat
      181 aacatgattt ctatttacgt gagcatgaat tttgttacgt ttttattgcc gaaaaataaa
      241 aaccgttcat tatgatgtca aattcgtcag taattgatgc aatgtgttat aataattcag
      301 ttaaatcaaa aatgaaatgg agaaaagaca tggacgggtt attaacttta tggaaagaac
      361 gcggaatgac aagtcatgat tgcgtattca aattaagaaa aatcttacat acgaaaaaaa
      421 tcggacatgg gggtacgctt gacccagacg tagacggtgt gttgcccatt tgtattggaa
      481 aagccacaaa agttatcgaa tatttgacag attctgggaa aatctatgta ggcgagataa
      541 ctctaggttt cagtacaacg acggaagatc gatccggaga acgggtagaa acaaaaccag
      601 tagatatgtc tttatctgaa gaaaaaatag atgaagtgat ggcttcattt attggtgaga
      661 tcacccagat accaccaatg tactcagcgg taaaagtcaa cggtaagcgg ttatatgaat
      721 atgcccgcaa taatgaaaca gtggagcgtc ctgttagaaa ggctcagatt taccgtttta
      781 tccggacaag tgaattgaca agagacgaag tgacaggaac catttcttgg cgcttcgaag
      841 tagaatgcgg gaaaggaacc tatgtccgca cattagctgt agatacaggc aagaaacttg
      901 gttaccctgc gcacatgtct gatctgaccc gaacagcaag tgccggtatg tcccagaaag
      961 atgctctgac attagaagaa gtagctcaag ctgtgaaaaa tgggacaatc gatgattgtt
     1021 tgctgccaat agaaaaagga gtgaaacgat tcaaacgagt agatattact gatgaagtat
     1081 ggcagaaagt aaaaaacggt atgagactta attatactgt atttgcgctc ccagaaatgc
     1141 cgtcagaaga aattgctttg ttttataaag gcaaagttgt cagtatctat cagccaaacc
     1201 cgaaagaaaa aaacaaatta aaacctagta aagtattgag aaatgaggaa taacgaatga
     1261 atatcatcaa gatcagacat ccttaccaat cagaacaaat tccagaagac gatgtcgttt
     1321 tagtactagg attttttgac ggcgtgcata ttggacacca gaaagtcata aagacaggta
     1381 tggaaattgc acaaaaagaa ggattgaaat tagcattgat gactttcaat cagcatccat
     1441 cgattgtctt taaaaagatc gatcctagca gcgtgaaata tttgactagc cttaagcaaa
     1501 aagaagaaaa aatggccgct ttgggtatag attatttata tgaagttgat tttacttctt
     1561 cttttgctca tttagcaccg caggaatttg ttgatcaata catcgtaggg ttacatgcaa
     1621 aatatgctgt ttccggtttt gattatacgt atggaccaaa agacattgca gatgtcgctc
     1681 attttcctga atatgccaaa ggaagattca aaatcgtcac agtttctaaa gaagaagaac
     1741 aaggtgaaaa aatcagctct acccgtattc gaggattact agacagcgga caagtaaaag
     1801 aagcagcaaa actattaggt tgcacatatg aggtagaagg ggtagttgtc catggcgatg
     1861 cacgaggcag actactagga ttccctacag caaatgtcaa agtaaagagc acagttcatt
     1921 tgcctaaaga aggtgtttat gtatcggaaa taaaaattgg ggaaatttgg tatccagcca
     1981 tgggttcaat tggacataat gatacattcg gtgaaggacg ggagctgact gtagaattgt
     2041 atattttgga ctttaatcaa gatatctatg gtgaacatgt cgcagtacgc tggaatcaat
     2101 ttttaagaga tcaagtgaaa ttttctagtg tagaggaatt gattgaacaa ctcaaagagg
     2161 atgaacaagc aacagctgaa tatttttctc agcaataaaa cattatgcat aaaatctctt
     2221 gtttatgtat aactagcaat ttcaataaaa tgggtgataa ataagcccag gaaaggggct
     2281 gtgacaaaag tcttgtcaca gccttttttt ccaaataaat ggtttttcag aagtagcttc
     2341 ttcggaaata agccgaaatt tcacaaaaat ttgagaagta atttttgtaa attacttctt
     2401 atttcttgaa gctggccact tctgtcacaa cctctttctt tatagaaata aaaggacact
     2461 agcaattcat gaaaacgatt gctaaagcgt atcatttgct ttagactata tatcttggtg
     2521 tacactcact ttaagacgga aataaaagtt atccgtaata acaggtttag gtatgaaaaa
     2581 ggagtgggta ctatgtataa ggaattagaa aacaaagtcg cagtagtaac tggtggctca
     2641 aaggggattg gaacagcgat tgctcaacga ttcgggaaag aaaaaatgaa tgtagttgtc
     2701 aactataact cggataaaga aggagcagaa aaagcagtca aagcaatcga agaggcaggc
     2761 ggaaaagcag tctctgtaca agcagatgtc agttctgaag aaggaataca gcgtttactt
     2821 gatacggctt tagaaaattt tgggacactg gacgtttgga ttaataatgc tgggatggaa
     2881 aatcagcatc caacccatga acttagcctt gaagattggg aaagagtatt aaaagtcaat
     2941 ttgactggcg tttttatcgg aacgaaagcg gctttaaatt actttacaga gaatgataaa
     3001 aaaggaaata tcatcaatat gtcttctgtt catgaacaaa tcccatggcc aacgtttgct
     3061 cattacgctg cttcaaaagg cggagtaaaa ctattcaccg aaacggtagc aatggagtac
     3121 gcaccaaagg gaatcagaat caatagtata ggacctggtg ctatccgtac acctattaat
     3181 gcggcaaaat ttgatgatcc tgaacaaaaa gaactcttgg aaagtatgat cccactagga
     3241 cgcgtaggtg aaccaaaaga aatagcggcg acagctgcat ggttggcttc agacgaatcc
     3301 agttatgtta caggtattac actatttgca gatggtggga tgactttata tccttctttc
     3361 caaggtggaa aaggataagg gtgaataaaa atgggtttat tgattgcatt gatcccggca
     3421 gttgggtggg ggatccagcc actgattttg aaaaagattg gtggcagacc aacaaatgag
     3481 atattaggaa ctggtattgg agccgtgctc gttggcttac ttgttcaatt attcgcttct
     3541 ccagctggta tttcagtgac tacatttttg attagtttac tttctggtgc attctgggtc
     3601 atcggacaaa caggacagta tacatctttc aacttgattg gtgtttctaa aacgatgcca
     3661 atttcgactg cattacagtt agtcggaacc tcattgattg gtgtcttcgc ttttggagaa
     3721 tggtctggaa gtatgggaaa aatcattggt gcagcagcaa tcattcttct tgtatttggt
     3781 tctgctttaa cggcaatcag tgatgatcct ggaaaaaaag gcggtatcac aaaaggaatc
     3841 agtctattag catcaacaag tattggttat tgggtttaca gtgcattgcc caaactagtt
     3901 gatgcaagcg gactttccat tttctttccg caaatgatcg gtgtcttttt aggggcagtg
     3961 atttatgtga ttttccgaaa accaaccgca ttttctgaag acaagagttg gaaagcaaca
     4021 atagttggag ccatcttcag tgtgtcagca ttggcttata ttttttcagc aaatgaaaat
     4081 ggggttgcta ctgcatatat cattacacag ctaaacgtag ttatctctac attaggcggt
     4141 atgtacatcc tgcatgaaaa taagacacca aaagaaaaaa aattaacgat tatcggatta
     4201 ttgctgatcg tagtaggaag catggtcacg gtctttattt gaggataaag atggtaaaat
     4261 ctcatttatt aaaaaaatat ttgtttgaac caaacagtaa gtacttataa aaaaatagcg
     4321 ctagaatttc caacatctct ggaatttcta gcgcttttta attgtttaag atacaagtga
     4381 aatatctgtc tgcatcaata atttttcatt gatccattga ctaaaattta cgacatgatt
     4441 gaagttacca ccgattagta tctccgcatg attgtcttta taaagatcgt attgtaaaag
     4501 acggtctttt ttatttgcgt aaacatcgtt atggtactgg actaattgtt ctcggatttg
     4561 attgattgct gtaaaatcac gatactgaag gttgaaacgc atcgtgattg gtgcatttag
     4621 catgaatcgc tgcatttctt ttgctgcttc ctctgcttga tctccgtata ggaactcaaa
     4681 aagattacgt tcagaaaatt tccattgccc gccaaccttt gttcctttta gttttccttc
     4741 atgaagataa tttctgatag aacgtgtagt aacacctaat tgttcggcta attcatttac
     4801 tgagtaaaaa tctttcaatg ttcaccctcc tatgtgaatg gttctactga cttagttgat
     4861 atttgcttaa tcacgaaatg tagaaagaac gattttaatt aaggtcattg taaaaatcaa
     4921 gtcagaaaat tctaactttt ctttctctaa agaaattcgt tctctcctaa agtgatatta
     4981 tatttaaata tacctgaaaa taatgggcag tattatgaaa gatcactgat attttctcgt
     5041 attttttcct tattcaaaag ttttgcagga aaattgctga aaaaataatt ttactaagtg
     5101 gaaaattcaa agccattcaa tcagtgattt ttttcagcac aagaaagact ccttataaaa
     5161 atagcagata ctttgtcaat taagtgttta accagggatg ataaaaagga taggattgaa
     5221 caaaacctgt ataagctgat gaatagtttc ctcgccagta aaaggtttat tcgtataaag
     5281 ctttatcgcc aaaatttatc atgacctttt ttatcttcaa tattaaatga aatcaattgt
     5341 tcaatcaatt cccagttgat tacacttttc caaggaactt tgatgacgtt atccgtatat
     5401 acgtagcctg cttcttggat atttttttca agggcacgaa tcgctgcttt ttccggtgcg
     5461 atagaaaaat gatttttagc aaaactaaat gcaataataa aggtaccatg gtcagtaaac
     5521 ataggttggt tccattttac agttgtgtct agctggggat acgtttctgt tatcttagca
     5581 aaaaggtctt tgatcttttg ttgatgttct ttttctttga tctgattgat gatcggctga
     5641 aatttttcca ttctaaaacg cctccattaa gtggtttgat ttattttacc agaaaaacaa
     5701 tagttatgta taaaaaacga acaaaacaca agtaatgttg taaccgctat atttatatga
     5761 taagatgtct caaataataa aatagaaaag agacataaaa atgattgact tggcaaagaa
     5821 attcttacac gaaaatatat attttgagtt gtatcatcat cgagccattt atacaaatga
     5881 agatgctttg gtaataaaga aagaacaagg atttacaggt acagagacca aaagcttatt
     5941 cttaaaagat aaaaaagata ataactacgt atatctgact tttactacga aacaaacgga
     6001 tttcaaagca ttagggaaga tcgtcggtaa aaggctttct attgtttcat cagaacgcat
     6061 ggaagaacaa acgggacaaa agcagggcgc tgtttctccg tttggttatg aaaaacagat
     6121 tccgattatt attgatgaag agctcttatc aaatgaaaaa cttgtttttg caccaggtag
     6181 accagatcaa acgatggtca tccaaacggt tgacttaaac aaaatcatcc agctacttga
     6241 gatgccgata tttttgatgc cggcaaacga aaaaaacaat taaccaagta aaatagtgca
     6301 tacttggagg tgaagcaaca tgataagtta tccattatta gaaagaaatg caacgattgg
     6361 ggttactgca ccatcctcag gcgtatcaga ggaacaccat tcgttactcg aatcagcttt
     6421 aaagagaatg gaagaaaaat ctttccaagt agttactgga aagacgcttt ggacgcaaga
     6481 taaggaaaaa tcaggtccag caagaaaacg agcagatgaa ttcaatcaaa tgatgcaaga
     6541 tgaaaatatc caaatgatct ttccgccttg gggaggagaa ttattgattg agattctcga
     6601 ttttgtcgat tttgatgctc tacaaccaaa atggatagta ggttattcag atattagtac
     6661 gttgctactt gctgtcacat taaaaacagg gatagccact gcacatggaa ccaaccttat
     6721 tgatttaaga ggagagtact tggatgaaac gacagcaatg tgggaaagtg tactgaaaac
     6781 aagagaagga gaatcgattc tacagcaacc ttctgaaaag tatcaaaaag agtggcccca
     6841 tgagcattca tcatcttctg ttttccatct cactgaacca acaaattggc aatcagtcag
     6901 cggagatgca gtaaggatga agggacgatt attaggtggc tgtattgata cgatcagtca
     6961 tttaatcggt accccttatg gagatgtcca atcttttaaa agaaagtata atgatggaaa
     7021 gccgatcttg tggtttctag aaaattgtga gctatcgtca gttgatttaa gaagatcact
     7081 tgttcagatg aaactggcag gatggtttga tggcagctcg gggatcatgt tcggtagaag
     7141 tgcagcagag gatttgttag attataaaat gaaggatgta tatgaagatc tgtcaaagga
     7201 attaaaacta ccgatcttgt atgatattga ttgtggtcat gtaccgccac aactgacatt
     7261 gatcaatggt gcctatgcag aagtagcggt agaagataga aaagcgactg ttttgcaaac
     7321 gtttatttaa aaaatttttt cctgccttgg agtttatcaa agaccaacaa atctaaatag
     7381 aaaagaaaat gaaagaagct gacagtattt tgccagcttc tttttgtatg atctattaga
     7441 aattttaatt atccataact atcacattga tcagctgttt cacatcattt ggattacctg
     7501 taggaatagc tactacagat gcattatcaa taatttcttg agttgtttct ctaatgaacg
     7561 ctggataaga tgtttgatct gcaggcattt gccaatgtga aggaactgga taactagtga
     7621 ttgtcccgtc atggttactt gaatacacta aaactccctg gtaactgtat ttcccagtga
     7681 gcataaccac atctttcgga tagactgcac tccctgcatc ataaggggcg ataggtgttc
     7741 ctgcaggata atgaataaca tgtagttcga aatcacccga tgtatctcta aaagtattac
     7801 ctctaattgc tagccaaaca cgtgcgtact caatttgtag atcagaatat cctgctaacg
     7861 gatctattga ctgagaagac tgtgaggaag ttgtgtctat tgttgaagaa gaaatatttg
     7921 tctcaactgt actactagat gtagaagaag tattgggctc tgagacttta ctgcttgttg
     7981 aagatgtatt cgtttcagta gaagctgatt tctgagtact tgatttttca gctgaactgg
     8041 tttgtttgat tgaacttttg tctgtggtcg cagatggatc actattttct ttactaggag
     8101 acgtacatcc tacaaggaat aaagagacca acaataaaat aaaacctaac tttttcatat
     8161 aaaatacctc attatttttt taataccagt atagcataat cctgaatttt tgaatccgta
     8221 aaaccttgtc t
//
LOCUS       DACCXO010000120         8120 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.123, whole genome shotgun sequence.
ACCESSION   DACCXO010000120 DACCXO010000000
VERSION     DACCXO010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.123"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(122..304)
                     /locus_tag="IVY70_002450"
                     /pseudo
     CDS             complement(122..304)
                     /locus_tag="IVY70_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            321..488
                     /locus_tag="IVY70_002451"
     CDS             321..488
                     /locus_tag="IVY70_002451"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959559.1"
                     /translation="MENTFELYNKTTVDKLIPILVPTDLNGHDVLLRFNGFLGEDYRY
                     LKISDPQPLTE"
     gene            complement(531..1106)
                     /locus_tag="IVY70_002452"
     CDS             complement(531..1106)
                     /locus_tag="IVY70_002452"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP7959560.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1261..1787)
                     /locus_tag="IVY70_002453"
                     /pseudo
     CDS             complement(1261..1787)
                     /locus_tag="IVY70_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            2244..3755
                     /gene="gadC"
                     /locus_tag="IVY70_002454"
     CDS             2244..3755
                     /gene="gadC"
                     /locus_tag="IVY70_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HAP7959561.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            3768..5168
                     /locus_tag="IVY70_002455"
     CDS             3768..5168
                     /locus_tag="IVY70_002455"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAP7959562.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5333..6768)
                     /locus_tag="IVY70_002456"
                     /pseudo
     CDS             complement(5333..6768)
                     /locus_tag="IVY70_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            7161..7754
                     /locus_tag="IVY70_002457"
     CDS             7161..7754
                     /locus_tag="IVY70_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HAP7959563.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVSGGMATVFLTNF
                     IRCRTSFRMLFRLSLTMSLLGCDDLRQELTVKVRLPR"
     gene            complement(7738..>8120)
                     /locus_tag="IVY70_002458"
     CDS             complement(7738..>8120)
                     /locus_tag="IVY70_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7959564.1"
                     /translation="FSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKG
                     CPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVG
                     YRNQRLAQRILDNELGCANASEAV"
BASE COUNT         2537 a         1393 c         1481 g         2709 t
ORIGIN      
        1 aaaatatatt acaataataa tcgtaatagc taatgaaaca atttctcaat ctttttacca
       61 ctccaaacag ggagcgtcaa taattttgtg taaataactc gtccttctgc aaaataatgg
      121 gttactcagt aaacattgaa gctaatgtat ccgtcacttg ttggaagcct ttatggcttc
      181 tatttaagaa tttttgattg tatgtatcaa agatggatac taggaaacgc tctagtgatt
      241 cttcattttg aaactgctct tttctgcggc tgtatttctt gatttgttta ttgaaagatt
      301 cgattgtaga accagaagga atggagaaca cttttgaact ttacaacaaa actactgttg
      361 ataaattaat tccaatttta gttccaactg atttaaatgg tcatgatgtt ttacttcgtt
      421 ttaacggatt tttaggtgag gattatagat acctaaaaat ttctgatcct caacctttaa
      481 cagaataata aaaaacgtgt aatcaattat ttgattacac gttttttatt tcaatcttcg
      541 ttgtagtagt ggttgcgaca taccgaaagg taatcttcat tacctcctac tacaatctgt
      601 tcacctgaat aaactggaat accatcaaca atacgagcat tcattacagc ctttttattg
      661 caaaaatggc aaatagtttt aatttcctca attttatcag caaatgtcaa taatgcttcg
      721 gatcccttga agagattatt ttgaaaatca ttcttcaatc catagcaaat tactggtact
      781 tcctcattca ttacgataag tgatagatca ataatattct ctctacttaa aaattgagac
      841 tcatcgacca aaatacaatc aacttttccg tactgatcaa taatctccac taattctccg
      901 tacacatcca tttcagaagt aattgcaata gctggtttcg aaacacctat acgactctca
      961 acttttccaa atcctgatcg agtatctatt gcgcttgtca ttatgataac tctcttccct
     1021 tgttcttcat aattatgagc cactttcagt agctcaatac tctttccact attcattgtt
     1081 ccatatctgt aaaataattg tgccattaaa tttattccct tcttttacaa ttcaattatt
     1141 ccttaattcc atctgctttt atgcagctgc tcgtttattc cttgatatgt ctataacatc
     1201 tactatccct aaaaccaatt tataagccat tagagatggt attcctgaac atctctcata
     1261 ttgttttttg agtggtatat catctgaaaa actgaaggaa tcatgaaaat agcgacatac
     1321 ttatcattta gagttctaac tttaaatgca tttacttctt cagtcagcac tttagtcatg
     1381 ttggacatgg tttggggcgt gtagtgatgc ccatacattt tctcaatcaa gtctgcgatt
     1441 tccgacatgg taataccttt tcggaagagg tgaatgacgg tctcctctaa tgtgtcattc
     1501 gtccgtctat aagcaggaac agtctgttgc ttgaactcgc cattgcggtc gcgcggaatc
     1561 tggagatgaa gttccccata ctcggtcttg accgtacggt cataggagcc gttacgagaa
     1621 ttacccgtgt taaaaccaat gcgatcgtac ttttcgtaat cgaggaaagc cgttaactcc
     1681 gttttgagaa gcgtattgac agctttttcc aaatggacac ggaataatcc attcaaatcg
     1741 cctttgttcg ctagagtctt taggatttct gtagtaaaat cgttcatatg gaagtcctct
     1801 tttctgtgaa tgtgttgtgg taactctatt ctacagaagg gacttccttt tttctattta
     1861 cacaaaatat tttacactct caacaaaagt atcttcgatt aatacttgct caagttatgt
     1921 gcagctacga aaaagtaggt accgaggtac tttctttttc gcagcttttt ttggacttat
     1981 gagaagtaca ttcgctggaa gaatacgata taaaacgagc tttttcacta aagatattaa
     2041 tagagtgaaa gaaacaagct tttgttgtca gttttttcac ttttctaaaa atgaaaataa
     2101 aaaattctgg aagtggagaa gaattttgct tgctatactt tgtcttgtag aaaagaggtg
     2161 gacaaaataa tgcaagctca aataagcgat tttccataac cttggtagga atgacaaatt
     2221 aaataggaaa gggaagtaat aatatgaata aaaaaaagtt atctctattc ggtttttttg
     2281 ccctgacagc ttccatggtt ttgactgttt atgagtatcc gacctttgca acatcacaat
     2341 tacatttggt tttctttctt cttttgggag gtattttatg gtttctacca gttgctttat
     2401 gtgctgcaga aatggcaaca gttgaaggat ggaaagatgg tgggattttc agttgggtta
     2461 gtcaaacttt aggagaacgt tttggttttg cagctatttt tttccaatgg tttcaaatta
     2521 cagtaggttt tattacaatg atctacttca tactaggagc gctgtcgtat gtaattaatt
     2581 ttccagagct taatacagat ccatttatca agtttattgg tgtattagtc attttctggg
     2641 tactgacttt ttcacagttg ggcgggacca atcgtaccgc taaaattgct aaagcaggat
     2701 ttgtcattgg aattgttatt ccttcgatca ttctctttgg tttagcagca gcctacttta
     2761 tcggcggtaa tccgattcaa atggctatta atgcgaaagc ttttattcct gacttttctc
     2821 agctttcaac tttagtagta tttgtatctt ttatattggc ttatatgggc gtagaagctt
     2881 cagcatcaca tattaatgag ctggaaaatc cccaaagaaa ctacccattg gcaatgtttt
     2941 tactagtgat tttggccatc gccttagatg caatcggcgg tttctcagta gcagcggtta
     3001 ttccacaaca tgacttgtct ctcagtgctg gtgtaatcca aacatttgaa acgcttattt
     3061 tacattttaa cagtcattta cattggttgg taaaagtgat tgctctcatg attgcctgtg
     3121 gcgttatggg tgaagttagt tcttgggtag ttggtccatc acgagggatg tttgcagctg
     3181 ctcagcgagg tctgttacca gttttcttcc ggaaaactaa taaacaagac gttccagttc
     3241 cgttgattat gatacaagga attatcgtta ctatttgggc agcagtctta acttttggtg
     3301 gaggaggcaa caatttatca ttcttagtag caatctcctt aactgttgtc atttatctcg
     3361 taggatattt actatttttc ctaggttact ttgtattagt ttttaagaaa aaagacttaa
     3421 aacgaactta caatgtacca ggaaaaactg ttggcaaaac gattatcggt ggaattggtt
     3481 ttattttatc agtgttcgca ttgcttatct cttttgtacc accagcttca attgcagcta
     3541 gtgagcaccg aacataccaa atcatactac ttattagctt tgtagtgact gttcttttac
     3601 cttttatcat ttatgaagta catgataaaa gtgctcacac aactattgag gaacctactc
     3661 acgtgaaagc tgaagacgtt aatcccgctg tttatccagc tgctagagga gagcaccacg
     3721 ttacaaaaaa tgatgaacat attttaaaac attaggagga attcattatg ttatacggaa
     3781 aagataatca agaagaaaaa aactatttgg aaccaatttt tggctctgca agtgaggatg
     3841 ttgacttgcc taaatataag ttaaacaaag aatccattga accacgaatt gcttatcaat
     3901 tagtacaaga cgagatgttg gatgaaggaa atgcgcgatt aaacttagct actttttgtc
     3961 aaacgtatat ggaacctgaa gcagtgaaat tgatgaccca aacgttagaa aaaaatgcaa
     4021 ttgataaatc agaataccca cgaacaacgg aaattgaaaa ccgctgtgta aatatgattg
     4081 ctgatttatg gcatgctcca aataatgaaa aattcatggg aacttcaacg atcggctctt
     4141 cagaagcctg catgctgggt ggtatggcca tgaaatttgc ttggcgtaaa cgtgctgaaa
     4201 aattaggtct tgatattcaa gcaaaaaaac ctaacctggt gatctcttct ggttaccaag
     4261 tttgttggga aaaattctgt gtatattggg atgtggaact gagagaagtc ccaatggatg
     4321 aaaaacatat gtcaattaat ctagatactg tcatggatta tgtggatgag tacacaattg
     4381 gtattgtagg tattatgggt attacttaca ctggtcgtta tgatgatatc aagggtctga
     4441 atgatttagt tgaagctcac aataaacaaa ctgactataa agtatacatt catgttgacg
     4501 ctgcatcggg tggcttttat gcaccattta ctgaacctga tctagtttgg gattttcaat
     4561 tgaaaaatgt tatctcaatt aattcttcag gtcacaaata tggtttggta tatccaggtg
     4621 tgggttgggt cttatggcgt gaccaacaat acttaccaga agaattagta tttaaagtta
     4681 gttacttagg tggagaaatg ccaactatgg ctatcaactt ctctcatagt gcagcacaac
     4741 taattgggca atactacaac tttgtacgct atggctttga tggttatcgt gatattcacc
     4801 aaagaactca tgatgttgct gtttatttag ccaaagagat cgaaaaaact ggtatttttg
     4861 aaatcattaa tgatggatca gaattgccag ttgtgtgcta taagctgaaa gaagatccca
     4921 atcgcgaatg gacactatat gatttatctg atcgtctgtt aatgaaggga tggcaagtcc
     4981 cagcctaccc attacctaaa gacttggatc aattaattat tcaacgctta gttgttcgag
     5041 cagactttgg aatgaacatg gctggtgatt atgtacaaga tatgaaccaa gcaattgaag
     5101 agttgaataa agctcatatt gtttatcata aaaaacagga tgtgaaaacg tacggcttta
     5161 ctcactaaaa ttcttcagag aaatataaaa gaagttaatt aactaataaa aggagttgtg
     5221 attaccacac agctccttaa atctatttga ataaatatat gatattcatt tgttacactc
     5281 tcaatcttag ctgttgttct attcatcatt actcttattg taagtagttc agtcaaattt
     5341 gcttgttcaa ttgatcgata acagtatgaa ttgtttgaaa agtaatcgga tatataattg
     5401 ttttctcact acctaagcct tcttggtata aagaactatc gcaaagtata atccgctgag
     5461 tacaaaagaa agtgtcgtct actatttctc taagttgttt acaaatgata ttactaatag
     5521 aaaagtgttc tcccaaaatg tcttctaact gttgtcgata gtataaatac ctaaagtctg
     5581 acggagctac tatgaacact tcaattggag atttccgttt atgctcattt aaaagactta
     5641 aactaatggt tagtttacga atagaattct cattccaacg cagaaataaa ttgttttgtt
     5701 ttcgccattg ttcaagaatt tcatgaactt ctaagtacaa agcgcgttgc tcttcattta
     5761 acaagtaagt cttatcaggt tgaaatatct gaatatcagc ccaaagtgag cggataaacg
     5821 ggagaagtgc tttatcaaaa agtatctgat tttcttcaga tatattcaga gataagataa
     5881 gtttttgact gagctcaatc accaaagggt tatctgctac atggttttga taaacaacgt
     5941 tcaagtcttt ttgtaacaat tcaaagttat cgcatagata atttctagag ttgaatagaa
     6001 agtagatata aaggcattca tcttcgtcat gacataaaga gaaagaaagt tgctctaatc
     6061 ctttctgtat tagctgaaac aagggagtat gtcgggcaac tcttttttct tcctgactaa
     6121 aatatattgg gttagtctta gatctctgca aaccgatagc gatacctaat aaaataaatt
     6181 gtttttcaac tgtagggtaa cttcttgaaa aaaatgtttt tctacataat caatcaattg
     6241 ttgaccgctt tgaataattg atttatttag ctggatcaac tctgtccgat ttgtctcata
     6301 gattatcgtt agtaacaaac ttcgataatc cttttcagga atttcaaaat ggccatcagc
     6361 atttaagtac tgcatttgtt taaaaaaatt aatcaactca tttcgtaagt tgtaaacttt
     6421 gcttaacgaa atgaaggtag tatcagctat attactccaa ttgtgttccc ctgataaata
     6481 atgtcctagt acattgaaaa agtcagagcg ctgatagatg agttgagtga gtgccaacct
     6541 cgaaaacgaa gagtcgaatg cgactgtaaa gctacttcta gatttctcca ctgaaataat
     6601 ttgctgatgg agtatgtcgg taatgcattc caagtcgtta gtgacagtga gtgggcaaac
     6661 gttcaattga tctgctattt cgcgaatctg aatggtccct ttttcgtgta attgctcaca
     6721 aatgtaaact tgacgaaaaa tacttttctc cagataggtt tccaacatag tactcctcct
     6781 agtcatttta tacataacta ttataactct accaatcgga atagtcctgt aatatattga
     6841 atcaaaaata taaattataa aataattaga tggggtattt ggtttaagta tcccttatat
     6901 agtaataaat ttataggtgt gattctcgaa ttaatacccg aaaatgtgag agaattttta
     6961 tggatttttt aggatacaaa ttaattattt ttgcttttat cctgattacc agtccagtac
     7021 tgtcggtatc ccattttgca gcaaaatcat gttgataaag tgacaagaag actgatgaga
     7081 tatattatcc aaattgagaa gataattaat cggattcttg agtataccct actttttaag
     7141 aaagtagggt atacaagcgt atgaataaag acataacttt ccctgtgatg gacgttcggc
     7201 ttgttccaat taccaaaata caggcgaacg attataatcc caatcgagta gcgtctccgg
     7261 aaatgaactt attggagctt tctatcattg aagatggctt tactcaacca ttagtttgtt
     7321 attacaagga aaaagaagag aagtatatct tggttgatgt gcttcatagg tatcgtgttg
     7381 ctaaggaaag actcaactta aaagttgtac cggtaacagt tattgataaa cccatagaag
     7441 aacgtatggc ttcaacaatt cgacacaatc gtgcaagagg gacccatgaa ataagaggaa
     7501 tggaaattgt catccaaaga ttagtaaaaa atggatggtc agacaagaga atatccaaag
     7561 aattaggcat ggacattgaa gaaatttttc gctttaagca agtgtctggt ggaatggcaa
     7621 cagttttttt gacaaatttt ataaggtgca gaacttcttt ccgtatgcta ttccgattgt
     7681 ccttgacaat gagcctcctc ggatgtgacg atcttcggca ggagctaaca gttaaagtta
     7741 gactgcctcg ctagcgttag cacatccgag ctcattatca aggattcgct gcgccaatct
     7801 ctggttacgg taaccaaccg gtgtctcgta gccaagtgta ccgtgcaacc gaaggtggtt
     7861 ccaccaattt acatagtcaa ataactccaa atccaattgt tgtaaggttt caaatgtgta
     7921 ttgatagaca aattctactt tcaacgactt ataagttgat tcagctacgg cattatcaaa
     7981 aggacagcct ttatgactca atgatcgatt gatgtcaaaa gttgttaata attcatcaat
     8041 agcttggtta tcaaactctt ttccacgatc agtatgaaaa atctcaacct ctgtcagagg
     8101 ttgtttgata cggctaaatg
//
LOCUS       DACCXO010000121         8090 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.124, whole genome shotgun sequence.
ACCESSION   DACCXO010000121 DACCXO010000000
VERSION     DACCXO010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.124"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(176..2347)
                     /gene="copB"
                     /locus_tag="IVY70_002459"
     CDS             complement(176..2347)
                     /gene="copB"
                     /locus_tag="IVY70_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP7959565.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2367..4553)
                     /locus_tag="IVY70_002460"
     CDS             complement(2367..4553)
                     /locus_tag="IVY70_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP7959566.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4553..4762)
                     /gene="copZ"
                     /locus_tag="IVY70_002461"
     CDS             complement(4553..4762)
                     /gene="copZ"
                     /locus_tag="IVY70_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP7959567.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4775..5215)
                     /locus_tag="IVY70_002462"
     CDS             complement(4775..5215)
                     /locus_tag="IVY70_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP7959568.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5289..5828)
                     /locus_tag="IVY70_002463"
     CDS             complement(5289..5828)
                     /locus_tag="IVY70_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAP7959569.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(6443..7909)
                     /locus_tag="IVY70_002464"
     CDS             complement(6443..7909)
                     /locus_tag="IVY70_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7959570.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         2213 a         1767 c         1402 g         2708 t
ORIGIN      
        1 gagtatccta cggatttaaa tcatttgaga acatgagaat tagaatcttt ttgatcaatc
       61 aattaatcaa agtaagataa caaaaaatct gagccagaat gaggttcatt ctgacccaga
      121 tttgattttg tacaacccat cagtccttat tgacaaagag cccctttttt cattattatt
      181 tgattttcaa tgtcaaagca tttagagcta caactaccgt acttaacgac atcaagacag
      241 caccaactgc tggacttaat atgataccaa ttggcgcaag aattccggct gctagcggaa
      301 ttgcaagaat attgtaacct gccccccacc agaggttctg gatcattttc tttcgcgttt
      361 gcttggctag atcaagaaaa tgcaaaatat cttttggatc gctgtttgtc aatacgacat
      421 ccgcagaatc gattgcgata tctgtccctg caccgattgc catcccgatt gaagcacggg
      481 ctaaacttgg cgcatcattg atcccatcac ccaccatgat tacttttttc ccttggtcaa
      541 ggtatttctg gacaacagct tctttatcat ctggtaataa accgccataa tattcttcga
      601 tccctaaata atctgctact gcttgggcag cgtttttatt gtcaccagta agcatgacag
      661 gagtgatttt tctttctttc aatgttcgga taaattcttt tgcttctgtt tttactacgt
      721 cacctaaagc aacgattgca gctagatgat tttctaaaat caggaagctg atggtatttc
      781 cttgttcttg gtaaggtttc aataatgctt gttctacgtt taatccgaga cgctcaactt
      841 ctttttcatt aactattttc acttgttgtt ttttgactgt tgcctctagc ccgactcctg
      901 aaagattttt tagattttca gcttgataag gcttgatttc atgttctttc aaataattca
      961 taatgccaac agccaatgga tgattggcat tcgcttccaa ggctcctaga tattgaagga
     1021 tttcattttt attgaacgca tcatcaagta cttcgacacc cgttacagta aattttcctt
     1081 ctgttagtgt tcctgttttg tccagcatga tcacatctag atcattggct tgctccagtg
     1141 cattgcggtt tttcaaaagt aatccgtttt tagctgctat tgatgtggag cgagcaacaa
     1201 ctaatgggat agctaaacct agagcatgag gacaggcgat gataaagact gttaccatac
     1261 gttctaatgc attaggaaga tctgtcaaga acaaccaggc gatgaatgcc aggataccca
     1321 caattaatgc gacataaaac agccattttg ccactttgtc agataatgac tctagcttgg
     1381 atttttctcc ttgtgctttt cgcaccattt ccataacctt cgctagataa ccattttcac
     1441 cagttccagt tacttcgatt tcgatcgttc catccccatt gatagaaccg ccaatcactt
     1501 tgtcattggc ttgttttttg attcctcttg attcgccagt cacagctgat tcgtcaacaa
     1561 ttgtttcacc tttcaagatt acaccatctg ttggcatttt atcaccagac cggacgatca
     1621 aacgatcacc ttcgtttact tctttcaatg aaactgtctc ttcttttcca tctttcgtca
     1681 agcgtttcac tgattctggc agtaattctg ctagtttttg taaagcattg ctggcattcg
     1741 acacagcatt catttcaatc cagtgaccta gtagcataat aacgatcaaa gtagccaatt
     1801 cccagaagaa atccatgacg tgtgtatgtg gattgagcag atttgcgata aacgaataga
     1861 cactataaat atatgcaact gtaatcccca tcgcgatcag tgtcatcata gcaggacttt
     1921 tttgtttcaa ttccatctta gctccactta aaaatggctg accgccatag ataaatagta
     1981 tagttgctaa gatcaatacg agccaatcag aaccaggaaa cgttacttga aatggaaatt
     2041 ccattcccat cattggtgaa aaaagaatga ttgggatagc taggataagc gatagccaaa
     2101 atttttgttt gaaattcccc atgtgcatgg aatgatccat gccgccgtgt tcgtgatcca
     2161 tgtgcatcga atggtccatt tgtttgctgt ttgctatatc tccatggtta tgtcccatgt
     2221 gcatggaatg atccatctga ctatggtcat gtttcatctc catctgtttt tcatgagatt
     2281 ctgtactttc tttgtttact acttcttttt tatgctcatg ttttttctcc atatcgttcc
     2341 cattcatttt tactccatcc cttctcttac tttttcatgt gtcgatttaa gctcagggag
     2401 ttcaacaata cactgacgga actaaacgcc atcgctcctc ctgcaatgat tggattcaaa
     2461 aagcctagtg cggcaaaagg aataccaatc gtattataga caaaagccca gaaaagattt
     2521 tgtttgattt ttctcaatgt caacctagac aactcgattg tttgtgcaat ggatgccaaa
     2581 ctgctattca tcagcgtcac atcggctgtt tccatcgcaa tgtccgttcc acttcccatt
     2641 gccataccga catcagctaa tgctaaggcc ggtgcatcat tgattccatc gcctgccata
     2701 ccgacttttt tgccatcttt ctgaagtttt tctacgtagg ctgctttatc ctctggaagt
     2761 acttcagcaa agatatgctt tggatcaata ccaacttgtt taccaatcgt ttcggctgcc
     2821 agtttgttgt ctcctgtcag cataaaaaca tctaatcctt tattctggag ctgcttgatt
     2881 gcttgtttgg cttctagttt gatttgatca gccacggcaa tccacccgat cacttgcttt
     2941 tcatcagcta aaaacatgac tgtttttccc tgctgttcta attctaacgc gtgttcttgg
     3001 tattcatcaa atgataggtt caattcaatt aatcgtttac gtgtacccgc aaaatagcgt
     3061 actccattaa tcgtaccgct gatcccagca cctgggtggg ctgtaaaatc aatcatatcg
     3121 tatgttgctg cttcttgcag ttttccgtat tccacgattg ctttacctag tggatgttca
     3181 gaagcttgtt ctaaagtata aaaaatagat aggacttctt ttgaaccgac tagatctgta
     3241 actttgggct ttccttctgt aatcgttcca gttttatcca aaacaatgct gtctaagtga
     3301 gcagctgctt ctaatgcttc tccgccttta attaaaattc catttcgtgc accaacaccc
     3361 gttcctacca taatagctgt tggggtcgct aaacctaatg cacatggaca cgcaatgact
     3421 aatacagaga cactatggat caatgcgagt tgccaatcac cagtgatcaa acctgtagct
     3481 atgagcgtga taaacgcgat gactaatacg atcggaacaa aaatccctga gatccgatct
     3541 gcgatttttt ggattggggc tttgctccct tgagcatctt ccaccatttg aatgatttgc
     3601 gctaatactg tctgcttgcc gacttgtgtg acttccatgt gtagcagacc attcgtattg
     3661 attgtcccgc caaaaagcgt gtcatccgga ttcttttcta caggaagatt ttcaccagta
     3721 agcatacttt catctatcgc actgcttcct gaaataattc gaccatctgc tggaatctgc
     3781 tcaccaggac ggatgactag ttgatcacca acgactactt cttcaatcgc taacgtttct
     3841 tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa gagacatcaa ttgtttgatt
     3901 gcattacctg ttttcgtttt tgctgtatgt tccaaatatt tgccgagcaa aatcagcgtg
     3961 atgatcatac tgctgctttc aaaataaaga tcttgggggt gtccacggaa aaatccgttg
     4021 taaatgctca atgcaaaggc agccgatgtt ccaattgcta ccaatacatc catatttggt
     4081 gctttcgtct tgattgcatg gtacgcacct ttataaaaac gagcaccgac ataaaattgg
     4141 acaggagcag acagcactaa ctggacgatt ggaaaatgaa aaaagtgaac aatcgccgca
     4201 tggcttccta acatcatagc aatcattgac aacatcaacg gcaaagtcaa aaccgtactc
     4261 aatataagat cgaatagcat ttttttcaaa tacgcttgct tttcttctgc aattttttgt
     4321 ttgtgggctt catcgtacaa aattgcacca taaccgatgt tttctacgct ttggattaat
     4381 ttttcagtgg tcgtcccttc atatttgaca cttgcttttt cagtggccaa gttgactgtg
     4441 gcattgacca caccaggttg tccgttcagc tctttttcga tcctagcaga acaatttgca
     4501 caagtcatac ctgtaatgac gaacgtatcc attttggttt ctgctgttgc catcaaatca
     4561 cctctgcttt ataacccaat ccattgattg cttgacaaat cttttcggat tgaacttttg
     4621 tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt tactttcact ttttgcacgc
     4681 cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca atgattgcag ctcatacctt
     4741 cgattgaaaa ttgttgtttc attttttctt cctcctattg atgttgttta cattcacatt
     4801 gacctgggat acagttacat tcgatcgtat ctactggttg cttttgttcc agttgtttca
     4861 aaatctcatt gatatcttct tgagtcaaag tggcttcttc tactagatcc gcaatcgtct
     4921 ctccaatcct ttttgcacag atatgggaaa aaagattttc cgtcgcactg cgaactgttt
     4981 ccatctccga gacagctgga tgataaatga atttttttcc ttcttgttct gtccagatca
     5041 cttctttttt taccaaacgt cccagcaaag ttttgatcgt tgctggtttc caatccatcg
     5101 aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg tccaagtgtc caaataacac
     5161 gcatgatctc ccactctgaa tcactgatct ttactggcgt tttgactttt tccatttatt
     5221 tcacaccttt cgtttacaaa tgtaatcatt agcttatgaa tacagattac gcttgtaaac
     5281 aaaaactgtc aattattttt gctctctatt ttcaatttag ttaaaaattc ttgtttctct
     5341 cgtcttgttt taaggatgat cagtttattt cttgcattag ctacttggat cagctgctgt
     5401 attttcggtt cattgttttc ttcaaaatgc cagataaaag ataaaaattc taagtattcc
     5461 ctgtttagtt tctctgaaaa tgattcaggc atatctggcc gagatttttt gtttatcttt
     5521 ctttttagag aacgccaaac tactcggtag atggcgagat aacgcggcac tttaagccaa
     5581 atgatcttat ctgcttcttt aatcctttcc tctaatgtag aagtataatt tccttcaaca
     5641 atccatgacg gattagatac cataaacgat tgctgttctt gcaaaaacca cttttttgcc
     5701 tgaatactgt agtctgttgt atgccataaa aggtctagcg gcagtagcgg aaagtcggtg
     5761 atttctttga tcttccttgc gagggtcgat tttcccgacc cactttgtcc aatcacgatg
     5821 atcttcattc ttctccctca cttctctttg gaatttggtt cgatcatacc acttataacg
     5881 ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa gtcccttgtt tcgaataaat
     5941 ggtgttcaat cagctgacct tcagcattaa ctcaaaaaac ggcaaaatat gaggagctat
     6001 tttttcgttt gttcctctaa tactcaggtc aaatcgctga ttttacaacc tcttccttaa
     6061 acggtgttct cctgccaaca cctcggtcac aaggcacaat agtcaacaaa catgagaagt
     6121 tgttttttga aattgctcct tgtgactcgg agttaaacgg caggctcact acctctttct
     6181 taaacggtgt tcaaaagctt gcatctgcgg taataagccc agcaaaacga aaaatatgag
     6241 gggctatttt tgtttggctg ttcttattac ttgatgcagg acagcttttt cacaacctct
     6301 gattcacggt gttccagaag tagcttctgc gaaaaacaag ccgaaacttc acaaaaattt
     6361 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
     6421 tcttgtaaca agtatttcta ttttaaggct gaaccactgt ttctggttca gtgatttctt
     6481 tgtttttgtc tcgacgagca attgctggca agatcatacc taataagatc aagacaattg
     6541 gtgtgatgat attcgtgaat aattggaacc accatgcttt aggatcagca gtataatcta
     6601 cttttggcac cattcctagg atacatgcaa acgcagtgaa ggcaaagcac caagcaccaa
     6661 agataaaacc gatttttgga tttttaacaa atttatattc agaattgaat ttcttataag
     6721 ctttattcaa catcatatat gccaagaaca cccataggta acgcattggc atgacaactg
     6781 agttcaagtt cgtcaaccat tttactaatt cattcatatc tttgatacca aagatcggca
     6841 acaaaataat gattcctact agtatacctg tcagaagata accattcacc aatgtacctt
     6901 tacgattacg tttacgtagc cagtttggta caaattctgg atccgcatct gctaacaaga
     6961 tttttagtgg tgcatcaatt gaaaaggcta atgcggagat ttgtccaatc atgtttgtca
     7021 atgcgtaaat gatcatcaat gagttaccga taccatagta attacctaaa atttgaaaag
     7081 cactgtatgc cccatttgcc atcaagtctt taggaatatt atcgctagca aacagcatac
     7141 ccattgcgac tgagcctaat acagcacaaa tccctaccat tgctgctaag aagatcatcc
     7201 ctttaggaaa ttcctttgca ggatttcttg tagagttgac ataaggggat attttttccg
     7261 ctcctccaac tgcaaatact agcatcgaaa cggttgtgaa ataactgaaa tcaaatgttg
     7321 gaatatatgt tttcacttga ttcatatgtg gtgtggcaaa ttccatcgtt ggattgatga
     7381 acggtgccgt tacagctaaa agaataaata agatcgacat gacgaacatc gctgttccgg
     7441 ccagtccgcc aatcactttc aaagtagaaa gtccttttgt tgataaatat aaaaaggcaa
     7501 gaaatataat aagtgaaata actgctacca tttgtactga catcgtattt acgatatttc
     7561 catttccttg gactgcccaa ccgccagcaa tcaggattgc ttgaggcttt tgtgccaaat
     7621 aaggaatatg gacgacccag taagtccaag cggcatagta tgccagtcgt ttattagatg
     7681 tcgtattttc gacccatgaa ctgacgcctc ctttactatc cttgaaggct gaccctaatt
     7741 gtccaacgat caatgcgtat ggaataaaat aaatggccaa aatcaatagc caagaggtga
     7801 caactgatac tccttgctga gcatagttat taactacatt ccccatgccc caaaccatgt
     7861 taaatgcaat taacgcaaca gtaaaccagc gtaattgttt gttgttcatt cgttctctcc
     7921 tttatgataa aaatttgtta tccagatgta atcatattca atttttaaca aacttacaag
     7981 acagtgttat tttatgtgtg tattctttta tttaatacac atcgtttttt catatttttt
     8041 ccatcaaaaa aataagttat tttcttttat ttcatttacc ttattgaatt
//
LOCUS       DACCXO010000122         8089 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.125, whole genome shotgun sequence.
ACCESSION   DACCXO010000122 DACCXO010000000
VERSION     DACCXO010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.125"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            10..1164
                     /locus_tag="IVY70_002465"
     CDS             10..1164
                     /locus_tag="IVY70_002465"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP7959571.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1186..1938)
                     /locus_tag="IVY70_002466"
     CDS             complement(1186..1938)
                     /locus_tag="IVY70_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7959572.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2302..4968)
                     /locus_tag="IVY70_002467"
     CDS             complement(2302..4968)
                     /locus_tag="IVY70_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7959573.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5309..6760
                     /gene="cls"
                     /locus_tag="IVY70_002468"
     CDS             5309..6760
                     /gene="cls"
                     /locus_tag="IVY70_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP7959574.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7089..7904)
                     /gene="thiD"
                     /locus_tag="IVY70_002469"
     CDS             complement(7089..7904)
                     /gene="thiD"
                     /locus_tag="IVY70_002469"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP7959575.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         2345 a         1688 c         1451 g         2605 t
ORIGIN      
        1 ggtgttgaaa tgaaaatagg gattgatcgt ctttcctttt ttattcctaa tttatattta
       61 gatatgacag agttggcaga aagccgtggg gatgatcctg caaaatacca tatagggatt
      121 ggtcaagacc aaatggcagt caatcgtgca aatgaagaca tcattacact aggagcaaat
      181 gctgccagca aaatcgtaac agaaaaagac cgtgagctaa tcgacatggt catcgtcgga
      241 acagaatccg ggattgatca ttcaaaagcg agtgcggtaa ttatccacca tttactgaaa
      301 atccaatctt ttgctcgttc ttttgaagta aaagaggctt gctacggtgg caccgcagct
      361 ttgcacatgg cgaaagaata tgtcaaaaat catccagaac gaaaagtact agtcatagca
      421 agtgatattg ctcgttacgg cttggcaagc ggtggtgaag tgacgcaagg tgtcggtgct
      481 gttgcgatga tgatcactca aaacccgcgt attttatcga ttgaagacga cagcgtattt
      541 ctgacagaag atatctatga tttctggcgt ccagattata gcgaatttcc tgttgttgat
      601 ggtcctttat ctaattctac gtatatcgaa tcattccaaa aagtttggaa tcgacataaa
      661 gaattgtcgg gtcgaggact cgaagattat caggcgattg ctttccatat tccgtatact
      721 aagatgggaa aaaaggcatt gcaaagcgta ttagaccaaa cagacgaaga aaatcaggaa
      781 cgtcttatgg ctcgctatga agaaagcatc cgttacagcc gacgaatcgg taatctttac
      841 actggttcat tatacctggg gctaacttct ctactggaaa attcgaaatc actacagcca
      901 ggagatcgca tcggtctatt cagctacggt tctggtgcag taagtgaatt ttttacaggt
      961 tatctggaag aaaactatca agaatatctc ttcgcacaat ctcatcaaga gatgctagat
     1021 tctcgaacac ggatcactgt tgacgaatat gaaacgattt tcagtgagac gcttcctgaa
     1081 cacggagaat gtgcagaata tacttcagat gtaccttttt cgattacaaa gatcgaaaat
     1141 gatattcgtt actacaaaat ataataaaat cataaaaaga acccttcacg agtcgtgaaa
     1201 ggttcttttt ttatctgcgg accaaaggag cagtctatag gtcagcttag tcgtactaag
     1261 gatttctcgt ttttcactga atagtttgcg aatgtacctg gtcacaacag gatcttttgc
     1321 gtacgcgtca tagtatagct tcatcaattc ttcagtaatt gattcctgtg taaaataagt
     1381 aaaatctttt ttcttcactt tgaatccttc atcaattaag tatgtttcaa attgatccat
     1441 tacaactggc gatgtattag gatcttcttc tatcattcct agttgctttt cgataggata
     1501 aaaaacatca tcgattgatt ccactactct gattatcacg caccatttgc ttgacttttt
     1561 catcaatttt tctaattgaa taagatgtac caatgctgga tttgctgctg taaaaaccat
     1621 ttcatatgtc tcatttgatt ggataaattc ttctaacgac tgtttgataa aggaaacatt
     1681 tggcaactca tttttttctg cttctttttt tgcattttct agcattcctg aagaaaaatc
     1741 gatcaaatca tagcgattaa tttcttttgc taaggaaaga aatctgccag atcctccagc
     1801 aacgtctgct gctgtttcag caggcaggac tttctccgtt ttcagataat tttttacatc
     1861 ttcagcaata gttacttgcg attcttgctg gatttcataa tattcttcag caaattcatc
     1921 ccattctttt tcattcattt gatttcctct aattttttgt tatatgtttt cacttttcgg
     1981 aataaatcat tctcctcttt tatctattct atctcttttg atgaataata aaatgattgg
     2041 ctttccgtct atcataaata aaaaaaggga ctgtgaccaa agtcgtgtca aagtccctct
     2101 ttccgaataa acagtgtcca atcagatgac cctcagtatt agctcaaaat gctgatttta
     2161 caacttcttc cttaaacggt gttctcctgc caactcttcg gtcacaagtc acaatattca
     2221 acaaacatga gaagctgttt gttgaaattg caccttgtga ctcagaatta aacggcaggc
     2281 tcacaacctc gttgattcgt tttatttatt tccttgtact cgtacaatga tcaatttagt
     2341 gatttccatc aaaacaattg tcacaagtgc gattcccata catgtaaaga cttctgtcaa
     2401 tccaaatgct gatgggatag agaagacttc tttcagtcct gggatcattg ttaagcagta
     2461 aagcgcgcta catacaacta cagctagtaa gacacttctg ttagagaaga accctgcttg
     2521 gatagctgtt tgtgtgtttg aacgtgctgg gaatgtttgc aatgtccgac accaaatcaa
     2581 tgttgaaaat gacatggcta caccgaattc aggtgaatgc tgcatgccaa gccattgagc
     2641 aaagataaca gcaatcccaa tcagcacacc acgatacgtt acagaaatca atgttttgtc
     2701 cgtaaagatt cctgaatttg gatctcttgg cgggcggttc atcgtagcag gttcagcttt
     2761 ttctgtacct aatgcaattg ctggcagtga atcattgact aaattgatga acaacagttg
     2821 taatgctgta aatggattgg accagtcagc aactaaggca aagatgatcg caatgatcgc
     2881 acctaaattt cctgcaaata gataagaaat agctttcttg atattatcaa acacattacg
     2941 tcctacttct actgcgctga caatagaagc aaagttatca tccgtcaata ccatcgctgc
     3001 tgcatctttt gccacatctg tacccgttcc catagcaatt ccgatgtctg cttgtttcaa
     3061 tgctggtgcg tcatttacac catcacctgt catagctgag atatgacctt tattttgcca
     3121 agcacgaacg atacgaattt tattctcagg tgaaacacgt gcataaacag tgactttttc
     3181 taaaacttcg tttagttgat cttctgtcaa ttcatctagt tccgtcccgg tcaaagccag
     3241 gtttccttct tcaaaaatcc cgatttgttt tgcgatagca acagctgttg ttttgtggtc
     3301 accagtaatc atgattgttt tgattcctgc acttcttgct tctgttaccg cctgagtcac
     3361 ttcagcacgt ggaggatcga tcatagcaag caaaccaaca aggatcaaat cgtcttcatc
     3421 ttcaaatgat aaatcaaaat tatcaagtgg acggtaagca aaagctagta cacgtaatgc
     3481 gcgtgttgaa aaagcttcat tttgctcttg gaattttttc atcaattcat ccgtcatcgg
     3541 aacgacttca ccatttagca atactttttt actgcgttgg aagacgatat ctggtccgcc
     3601 tttaacaaac aagtaatttt tgttatcaat tgtgtgtcca gtagacatta attttcgatc
     3661 tgagtcaaaa ggaagttctg cttgtcttgg gtagttgttt cggatttcct gataaggctg
     3721 gtttaatttt tcactaaagt cgataaacgc tacttccgta gggtcgccca atttagtacc
     3781 gtcttcactg atcgatgcat cattcgctaa aacactgatt tcgatcaagc gacgttcgtc
     3841 agctgtccat tttgaagggt catttgtgaa atccccagta tcgccattac ctaaaaagta
     3901 gtcaacaact gtcattttgt tttgtgtcaa tgtaccagtc ttatctgtac agatgatact
     3961 tgtcgaacca agtgtttcaa ctgctggaag cttccgaata atggcatgac gttttgccat
     4021 tttattcgtt cccaaagata acacgattgt tacgatagac tgtaatgctt ctggaatagc
     4081 agctacggct acggctacgg caaacatcac tgcgctgacg atatctgcag tcatatctcc
     4141 tgtaccttct ccaagataaa tacgaaacag ttggatacct aaaatcaata aagacaatgc
     4201 caaaatagcc aaacttagtt ttttactgaa tttatccaga cctcgttgta acggagtgac
     4261 ttgctcagtt gttgattcca aaaggccagc aacttgtccg atctccgtat tattcccagt
     4321 tgaagtcact aagaatttac cgcgtccgta agttacgatc gttccactga ataccatatt
     4381 tgtacgatct ccgattggta ccgtacttgt gatatccgtt acttcttttt ctgcagaagt
     4441 cgactctcct gtcagcatac cttcatctac ttttaatgat cccgcatcta gtaaacgtcc
     4501 atcagcaggt acataatcgc ctgcgtctaa caacacgata tctcctacca caagttcgtt
     4561 tgccggaatc gttttttcct taccatcacg aatcactcga gcatcgggcg ctgaaaggtt
     4621 tttcaacgct tctaaagaac cttctgcttt ttttgtttgg acaacactga ttatagaatt
     4681 caacatcaat actgcaaaaa taactaagaa ttcgatgtag tcacccatga ccatctgaat
     4741 cagtgcgaca atcagcaata cgataaccat tgcatctttg aaagtttcta agaacaattt
     4801 ccaagttggc ttcttcggct tttcttttag ctggttcaag ccatctcttt ctaatttttc
     4861 cttcgcttgc gtggtgctca atccatcggt tgagctctgc gtttgctgca tcaacgagtc
     4921 gatcgtttct ttatagaccg cttgttgtgc ttgttccttg ttttccaaat ttcatccccc
     4981 attttttgat cttggcccta gtcctgtctg aaaaaaaaga gacctaagac aaatacgcat
     5041 ttgtcttagg tctcaccatt tcatacaata ccagaaaacc atttggttaa cttactgttg
     5101 atattatcgc aacaaacgtt gccggttact cccctaagga gatattcaac tgtactaata
     5161 tcctacctta ttaacctaca aaaagcaact actttagccc gatttaccta cattttcatg
     5221 aaaggagtga aaagtatatt caccttggta ggcttctttc ttctatcatg ctatactaat
     5281 gttattgtat gtaaggagga acatttttgt ggtatctagt attataaccg ccctttatct
     5341 attaaatgca cttattgctt tggtggctat tttgatcaaa ccccgagatg tagcagccat
     5401 ttgggcatgg ctcttagtat tgtttgccct tcctggggtg ggctttgtat tatatttatt
     5461 tttcggacgt ggattaacgg ataagaaaaa attctatctc cgacaaagtg acttgaaaga
     5521 attagaaaac tttcagtctt ttagaggaga taccattgaa cattacgatc ctgacatggg
     5581 cgataaagac aaacagcaat ttgttgactt cttctcttca ttaaatcgta tgccgctgac
     5641 aagaatgaat tctgtcactc ttctcacaga cggacaagag aaattggatt cactgcttca
     5701 agatctaaaa aaagccaaac attcgatcca tatcgaatat tacgcatttg tgacagataa
     5761 tatcggccag caagtcttac atgttttaga agaaaaagcc gcagaaggcg tggaagttcg
     5821 aatattatat gatgcatttg gctctcatgg cacaaaagca aaagatttca atcgtctaat
     5881 caaaaatggt ggacatgtcc atacatttgt tacctcacaa agggcattac ttcgtttccg
     5941 attgaattac catgatcacc gaaaaatcgt tgtgatcgat ggaaagatta gttataccgg
     6001 tggtttcaat attgccaatc aatatgtaaa tacaacaaaa aaattcggct attggcgcga
     6061 tacgcatata cggattttcg gtgccgcttc ttctttgctc cagcttcgct tcttaacaga
     6121 ctggaacgtc tcggtacctg aagaaaaaaa ggtcggctat catttgaatt atttctttaa
     6181 aaaagcagat cgagatgaat ctaagcttgc tgatacatcc atccagcttg tttcaagcgg
     6241 accgaataac gaaagggaac aaatcaagct ttcatttatc aaattgatta cttctgctaa
     6301 aaaacgtgtt tggatacaga caccttacct tgttcctgat gaaagtgtca ttgctgcttt
     6361 aaaaatcgca actgcctctg gtgtagatgt gaaaatcatg attcccaaca aaccggatca
     6421 tccttttatt tatcgagcaa cacaatatta tgctcggcag ctgatcaagg aaaatgtaca
     6481 aatccttgtc tatgagaacg gcttcctcca tgcaaaaaca ttgataatgg atgatgaaat
     6541 ctgcatggta ggttcagcaa atcaagatat tcgtagctac cgattgaatt ttgaaacaag
     6601 tgctgtcatt tacgatcctg agtttttaga agaacttgct actcagttca aagaagatga
     6661 gacacattgt tcatccatga caactgaaac agtcaaggaa atgtctaact ggctattatt
     6721 caagcaacaa atttctcgat tattttctcc aatcctataa tttttttatt caagtagaga
     6781 aaaatatact aagattaaag ggactgtgac aaaaatcttg tcacagtccc ttcttccgaa
     6841 caaacggtgt tctcctgcca actcctcggt cacaagtcac aatattcaac aaacatgaga
     6901 agctgttttt tgaacttgtt ccttgtgact cggagctgga cggcaggctc acgacctctt
     6961 tcttaaacgg tgttcaaaag cttgcatctg cggtaataag tccaaccaag ttaaaaacat
     7021 aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcaactt tttcacaact
     7081 tctttctttt actctaattc accagctgcc caatgattga tggggccata tttgtgccct
     7141 acatttattt cttcacgaat tgcacggttt acatagtctt ttgccaatcg gatcgcttgt
     7201 tcaactgttt ttcctttcgc taactcagct gcaatacatg cagatagcga atcgccagtt
     7261 ccgttgatcc ggtcggtagg ataaaatggt gcatttaacc aaaatgattt tcctgattcg
     7321 aacaatacgt agtcattgac tgtttcttgc tgcgcatttt tagcatgatg tcctttgatc
     7381 ataacgtttt ttgcacccat atcttgcagt tcttttgctg cttgcacaaa atcttcttca
     7441 gctgctaatg tccgtccagt cagcttttct gcttcataaa aattaggtgt cagtacttcg
     7501 gccaaaggga caagcttttc tttcaagctt tgcaaagcag attcttctag taaaagattt
     7561 ccatgtttcg ttacgattac gggatccacg actaatggtc caaaatcata tttttggtaa
     7621 ttttttacaa ctgtctcaat caactttgaa tctgctagca tacctgtttt agctgcacgt
     7681 atttgaaagt ccttcgcaat cagttcaaat tctttgtcga taaaatctgt tggcaatgtc
     7741 acacttgctc cgattccgta agaattacct gcaacacatg cggtcatgac agaaacaccg
     7801 taaacatttc ttgtaaagaa tgtatgcata tctgcttgca tccctgcgct tccatcgcta
     7861 tctgatcctg cgatcgtcat tgcttgtaca tactgatctt ccatcaaatc gattcctcca
     7921 tttttataaa aagatttttc ataatactgt tactgtttct atcatatgtt tttcaaagta
     7981 tccctcagta aatataccac taccttttct tggtaatcaa atttatttga aaaaacattt
     8041 gcaataaatc agaaaatagt ggtcgtttat ttgtattcgc tattctaat
//
LOCUS       DACCXO010000123         8003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.127, whole genome shotgun sequence.
ACCESSION   DACCXO010000123 DACCXO010000000
VERSION     DACCXO010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.127"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            79..780
                     /locus_tag="IVY70_002470"
     CDS             79..780
                     /locus_tag="IVY70_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP7959576.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1352..2368)
                     /gene="tsaD"
                     /locus_tag="IVY70_002471"
     CDS             complement(1352..2368)
                     /gene="tsaD"
                     /locus_tag="IVY70_002471"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP7959577.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2365..2829)
                     /gene="rimI"
                     /locus_tag="IVY70_002472"
     CDS             complement(2365..2829)
                     /gene="rimI"
                     /locus_tag="IVY70_002472"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP7959578.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2836..3378)
                     /gene="rimI"
                     /locus_tag="IVY70_002473"
     CDS             complement(2836..3378)
                     /gene="rimI"
                     /locus_tag="IVY70_002473"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP7959579.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3362..4186)
                     /gene="tsaB"
                     /locus_tag="IVY70_002474"
     CDS             complement(3362..4186)
                     /gene="tsaB"
                     /locus_tag="IVY70_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP7959580.1"
                     /translation="MYHCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDKIIVPMFDARRKNVYAGAY
                     RWKENRLEIVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFYEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(4275..5255)
                     /locus_tag="IVY70_002475"
     CDS             complement(4275..5255)
                     /locus_tag="IVY70_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7959581.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5279..6763)
                     /gene="galT"
                     /locus_tag="IVY70_002476"
     CDS             complement(5279..6763)
                     /gene="galT"
                     /locus_tag="IVY70_002476"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP7959582.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6775..7764)
                     /gene="galE"
                     /locus_tag="IVY70_002477"
     CDS             complement(6775..7764)
                     /gene="galE"
                     /locus_tag="IVY70_002477"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP7959583.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2260 a         1710 c         1406 g         2627 t
ORIGIN      
        1 gttggtatgt taaaacttta caaattcttt aaaaaaagtg acaataaata tagtgaacaa
       61 taaggagagg actttttaat gcgtgttaat aaaatcgatt ttcacttttc aaaactcaga
      121 tatcaacctg attttgaaca tttctcggat gaagagttcc aacagatcaa ggaacatact
      181 gttcttcgtt cttataaaaa aggacagatc cttttcgacg atggtgatcc cagacaacgg
      241 ttgtatatct tgaccaaagg actcgttcgt ttagaacgtt atgatgaaag tgcaacttat
      301 tactactatg actatgtgaa tacaaataac ctttttccta tgtcaggaat gtttggagaa
      361 gagacctaca cttatacagc tcaagcgatg actgatattg aaacattcta ccttcctgtt
      421 tcctactatg aaaaattagc acgaaaaaat cccgaacagt tgatctatat gatacaaaaa
      481 atttctaaag tagttgaaat acatgaattg cgtatccaaa tcggattgac ctctagcgcc
      541 tttgaccggg taaaacaaaa tttattcatt ttgaaagaag aattgggcgt accaacaaaa
      601 gatggtcata tcaccattcc ttatccgatc accttaaaag aattagcagt gaatagcggc
      661 acgacacgtg aaactgctgg acaagtaatc aaacaactac gtgattccgg actgttagat
      721 tatgacaaaa agcgttttac tttctgtaag acagctacta ctccagctat gagagaataa
      781 cgcgaacaaa aataagcagt gaccctgata tttatcaggg tcactgctta ttttcctccc
      841 taaaacttct gaaagatccg gcagttttag atcactgggc aagactcaaa gattttctat
      901 gtgtgtttca gttatgtaat attcactgtt tgcaaaaaat actgatagat taactagagg
      961 aagcgtcttg tcctcatccg acctagatta ttcgatattc ctcgacaata aacgattaat
     1021 cagtgagggc aatcagagga acggataatg atgaataagc tgtaaaaaat agggctttga
     1081 caaaagcgtg tcacagccct tatttcgatt aaacggtgtg tacctgtcaa ctcttcggtc
     1141 acaagtcata atgttcataa acatgaggag ctgttttttg aaattgttcc ttgtgaatca
     1201 gagttaaacg acaggcttac gaactcttga ttaaacggcg ttcaaaagct tgcatacgcg
     1261 gtaataagaa cagccaggtc aaaaacatga gaagccgttt ttcctgggct gttcttatta
     1321 cttgatgcag ggcagatttt tcacaacctc tttattcaga taaaaccaag cttggatcag
     1381 cattcaactg atagctagca aaatttcttt tttgcatttc cacatgtgct gctgctccaa
     1441 tcattgcagc attatctcca caaagtcgaa gagggggaat cactagttct acctcaggga
     1501 gtttagttgc caaagcatcc tgcaatcctt cacgcaaccc ttgattggcc gctacacctc
     1561 cagctacaat cagttgcttg actggataat tttgacaagc tcgaagtgtt ttagtaatca
     1621 atacatcgat cacacttgct tgaaagctag ctgctaaatc atttttgtct agatcttctc
     1681 cacgctgttg tgcattatgc accaaattga taaatgcact ttttaatccg ctaaaactga
     1741 aatcataatt atcttcatgg atcatcgctc gtggaaaatg gtaattatct tttccctgat
     1801 gagctagctg atcgatttct tttccgcttg ggtaagaaag acctaatact cttccaactt
     1861 tgtcataagc ttctcctgct gcgtcatctc ttgtctcccc aatgatttca tatgatccat
     1921 cttcttgcat atagactaat tctgtatgtc ctccgctaac aagaagtgcc atcaatggaa
     1981 actggaatgg tttaaccaga cgggctgcat agatatgccc tgccatatgg tttacaggaa
     2041 taagcggcaa ttggtgtgcc caagcaaaag ccttagctgc agaaatacca atcagcaacg
     2101 agccgacaag tcctggtcca taagtgacag cgactgccga taaatcttct gccgacacac
     2161 cagcttctac cagtgcatct tccaagcata aggtgatttg ttctacgtga tggcggctag
     2221 ctacttcagg aactacgcca ccaaaacgtt tatgactgtt tatttgagaa gcaacgatgt
     2281 tagataagat ctctgttcca tttctaacga ccgctacact cgtttcatca caacttgatt
     2341 caatcgccag aatcaattct tcattcattt catcgtctcc gtttttaact ttctgctcat
     2401 aatgatcgca tcttccgtcg gattgcgata atatgatttc cgtttaccta atatacgaaa
     2461 cttttctgat tcataaagtt ttcttgctgc ttcattagat agacgtactt ctaaaaaaac
     2521 atgcagcgaa tcttttattt gttccgtaat cagtaattgt aacaaatgcc gtgcatgccc
     2581 ttttccttgt tctgaaactg caacagcaat atttgtgatc tcgacttcat ctattacttt
     2641 cgagtatcca ataaagccac agatctggtt atcttcaact aaaagcagat aatcagagtg
     2701 aggctgttgg atgtctgcca aaaactgcat tttcgtccaa ggtgaaccat gtggataagc
     2761 attttcgcta atcatccata cgcattctgc caagtacgct tcatcaaaat ctttttttct
     2821 gcaaatcaac taatctcact cattttctaa aagttttatc atatccaacg catcttcttt
     2881 tgtttcagtg taatatcttt ttttgatcgt tgttgcttga aaacctaact tccgataaag
     2941 tctttgtgca tcttgattac tcatacgcac ttctaatgac atcgtctcac agcgattcat
     3001 tatagcaaag ttctcgattt cgtcaataag cagcgaaccg attcgtttac gctgatatgc
     3061 cggtcgaacg gcaatattcg tgatatggca atctaaaccg aacgctcttg aaccgataaa
     3121 accaatcatt cttccattat caaagatacc tagatataga tgaggaagcg gtgacttcaa
     3181 ttctgatata aaggcacttt ttgtccatgg caattcgcca ttatagactt ctcgttcgac
     3241 atccagcagc tctttgatat cttctgtagt caattctctt agtacgtaac gacgatcgat
     3301 ttgttcgatt gttttttctg tataaatcat tttatcttga aaaaagcttc tggcatacgc
     3361 tctaaatttt ttcaacataa ctttcgtctc caggtgtgtg cgtctccaac catttttctt
     3421 ctgcttctac ccgttttaaa tagcgaggaa gaaaagcttg tttctctgca actggttcag
     3481 ctaaagcacc taattgagcc acagttacac cacttggaat atcccatgct ggaactgtat
     3541 taatcatcgc actaggcaat atttccttta tttcatagaa gaatttgtga caatcactcc
     3601 cgacgaaata aatagcttca ttcagctttt gtagttgtgt gaacaaatca gaagcagaaa
     3661 tatgtacatc ttcaagtaca atttctaatc tattttcttt ccagcggtat gccccagcgt
     3721 atacattttt tcttcgagca tcaaacatgg gcacaataat tttatccaca ccgatgcaat
     3781 tagccgcgat ggttttcaga ctagatactc ctactagctc tttatctaat gtataagcaa
     3841 tcgtttttgc tgtcgttaca cctatacgaa gccctgtata agaaccggga ccatcagaaa
     3901 ccgcgattcg gtcaatgtct tttggactta tttgaagatc tgcaaacaat tggtcgattg
     3961 caggcatcag tgttacactg tggttgcgtt tgatatttgt ttgtatttgt ccaagaattt
     4021 gttcgtcttc taccacaccg atcgccaaag tttggtttgc agtatctatt cctaatgtaa
     4081 tcatatattt gatcctttct gctatgacat tctcgccttc cattgtagca tactttttta
     4141 ttctgaggat gaaaatacca aaggcaaaac tagtacaatg atacatgatt atctgctggc
     4201 gggcaaattt tgccccttcc tgaaacacaa aaaagctgcg gtatcacttc atttccccac
     4261 agcttttcca tcttttattt cgttgattct cgaataatca actctgtttc cacaataatc
     4321 tttcttgaaa ccggtgcttt ggaattactt aattgaagaa tcgtctccac tgctagctct
     4381 cccatccatt cggtataaac ttttacagta gaaagagagg gcgttacata tttcgctaca
     4441 ctgacatcat tgaaacccat cacagaaata tcttctggta ccactaaacc tttttcttga
     4501 atcgctttca gtgcgccaat cgctaatgca tcattagctg caaaaagcgc atcaggaaca
     4561 tgcggattcg tttttaaata ctgcttcact gcttgatatc ctgactcgac agaaaaatct
     4621 gcttcgataa ttggaccttc gtcgtagcct ttttctgcca aaacttgttg aaatgccata
     4681 attctgggat cttttagccg ctgatggttt ttcttcgtat attctgttcc agcaagcata
     4741 gcgatatgtt gcgctttatg ggagacaaaa tgttcaatga ctttttgaac acttgactta
     4801 aagtcgacaa ctagagagtc gattccttga gaagtgccgt cagaatctac caaaagcaag
     4861 gcattttggg atcctttcat ttctaataac tgagcttcgt cgaattttcc tagtgcgatc
     4921 gttccatctg tcggaagatc actaatggaa tcccacgttt ctttcaataa agtaacgttt
     4981 gcttcttctg cctttttttc gattcctaga cgaatagata aataataaag atcttcaagc
     5041 tcttccgtat cgtcgtacca ctggatcagg cgaagcgttt gcttagtatt tgctgcattt
     5101 ttacgatgtt tcgtataatt taagcgttct gctgcttcaa aaattttttg ctttgtggct
     5161 aagccaacgg aaagatcagg atcatagttg agaactcttg aaacagtagc aggagaaact
     5221 cctgccaact gcgcaatgtc tttaattgtt gccatttgtc tctcctcctt ttttacgttt
     5281 ataaaattgc agtaaatcgt tcaaacgctg cctgtccttt ttcatctcgc ttgaatactc
     5341 cagcgtcttc cagtacacgt gcaaaaacct gacctacaga tttttgtacg acatcggtga
     5401 ccgtatcttt cgtcaaatca ggatgttctt tttttaattg ttctgcccac ggacgatgat
     5461 attcagccat ctggttttct tctcctaata agaaatgctc tacttctact agttctggtt
     5521 tcaaacgagg tggtaaaaca gcaagcccca ttacttcgat caacccaatg ttctcctttt
     5581 tgatatgttg gacatcttta tgtggatgaa aaatcccatc aggatgttct tctgaaacat
     5641 tgttgtctcg aaggacaagg tccaactcaa agtaattgtc ccttcttcgt gcaattggcg
     5701 taatcgtatg gtgtggtgta ccatctgctg aaaaagctct gatagatact gtttcatccg
     5761 aatatgcttt ccacttttcc aatactgagt tagctgcttc aatcaacaat tttttctctg
     5821 ttgattgcaa acggatcaca gacatcggcc atttcacgac tcctgcgatg acttgcggat
     5881 aatctgctag tttgataagt ttttctatcg gtgcttttgc cataggaaat tcatgacgtc
     5941 ctgcctgata atgatcatga gaaagaatcg atcctccaac aatcggcaag tcagcattcg
     6001 aaccaacaaa gtaatgaggc aagacttcag taatccgaag cagtctagca aatgtttcct
     6061 ttgtgatttt catcgggcgg tgttgttctg ataaaatgat cgaatgttca ttgtaataag
     6121 cataaggaga atattggaat ccccagcttt caccgtctag attcattcga ataatccggt
     6181 gattggtgcg ggctggatag ttcaagcgac ctctatatcc ttcgttttcc atgcaaagca
     6241 tgcaggtagg atagtctact tttaccgcct cgcgttctgc tgcgatttgt tttggatctt
     6301 tttctggctt agataaattg attgtgattt ccagttcacc atactccgac tctgccggaa
     6361 atttgatgtt ttttgcgatg gctcttgttt tgatgtagtc atttttctta cttaaattaa
     6421 agaaatactc agttgcttcc tctgggcttt tcgaatagtg ctgtgcaaag aatgcattga
     6481 cgactgaagg tggcggtgtg agaaaatcca ttaattgcgc ttcaaaaatc tcttgttctg
     6541 ctggcaattc tgaaagaaca ccattttctc ttgccttagc gacaagctga tccaataagt
     6601 ctacagattc ttccttaact ggtcttactt caatgctttc tagagcatct tctcctacca
     6661 tccctaaaag acgattctgt aaatataagc ggtccatttc catccatccg ccagcttcga
     6721 tcgcaagtgt ggcaaaatca caaatcgttt gactgattga catatctcat tcccctattc
     6781 ttcataacca tttggatggc tgacatgcca atcccatgct gttttgataa ttgctttgat
     6841 atctgtgtat tcaggtttcc aacccagaac ctctctcgct ttttcacttg aagcgactaa
     6901 tcggctagga tctccagctc ttcttggtgc tatttctgct ggaatttctt ttcctgtcac
     6961 ttctctagct gcttctagca tttccttgac tgagtatcct ttgctgctgc ctaaattgaa
     7021 gaagttgctt tcatttcctt ctttcaagta ttccaaagct aaaatatgtg cagcaatcaa
     7081 atcttctact tgaacataat ctctgataca tgttccatct ggtgtatcat aatcgtctcc
     7141 ataaacagcc aatgcttttc tttgaccaag agctacttgt aaaatgattg ggacaagatg
     7201 cgtttctggt gtatgatctt ctccgataga tgcatctgct tttgctccgg ctacattgaa
     7261 gtagcgcaaa gcgacatagc gcatgccata tgcttggtca caccacttca tcattttttc
     7321 catcatcaat ttactttcac catacggatt tttaggatta gctggtgtat cttctgtgat
     7381 tggagactct tttggttctc cgtaagttgc tgcagtagaa gagaacacga ttttattcac
     7441 attgaactcg tgcatgactt caagtaagat ttgcataccg tatacgttgt tgttgaaata
     7501 catcaatggt ttttcgacag attctcctac taaagagctt gcagcaaaat ggattactcc
     7561 ttcaatcggt tccttttcaa atacggaacg tagaaattct ttatcccgga tgtctccttc
     7621 ataaaaatga gcatctggat gtactgcttg tttgtgacct gtgagcaaat tatctaccac
     7681 agctacttga tacccctttt gaactagctg gtctacagcg tgcgaaccga tataaccggc
     7741 tccgcctagt actaaaattg tcataaaaaa cactcctttg attagtaaaa ttgtgtttgt
     7801 tgattatata taatttaaaa aaacgaagtt tacttttatg gtgaaaacaa acaaagtaaa
     7861 atttcgtaaa ttcatatggt aaatttataa taaaacattt agtaaacatt ttcaaggatt
     7921 ttttgcaaag gtttacaaac aaacgtaaaa ctagcctttt tttctctttt gttccataat
     7981 agatataaca aataataaat gag
//
LOCUS       DACCXO010000124         7968 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.128, whole genome shotgun sequence.
ACCESSION   DACCXO010000124 DACCXO010000000
VERSION     DACCXO010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.128"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            285..2051
                     /locus_tag="IVY70_002478"
     CDS             285..2051
                     /locus_tag="IVY70_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP7959584.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2153..3412
                     /locus_tag="IVY70_002479"
     CDS             2153..3412
                     /locus_tag="IVY70_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7959585.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3559..4860
                     /locus_tag="IVY70_002480"
     CDS             3559..4860
                     /locus_tag="IVY70_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7959586.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4863..5714
                     /locus_tag="IVY70_002481"
     CDS             4863..5714
                     /locus_tag="IVY70_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7959587.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5727..6542
                     /locus_tag="IVY70_002482"
     CDS             5727..6542
                     /locus_tag="IVY70_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP7959588.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6693..7859
                     /locus_tag="IVY70_002483"
     CDS             6693..7859
                     /locus_tag="IVY70_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7959589.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         2601 a         1376 c         1589 g         2402 t
ORIGIN      
        1 ttatgtttca ttcttctaga atatacttct acgccaaaaa ctgttaaaaa acttttactt
       61 tagaaaattg gagaaattga tcgcttaaga taaattcagg acactaaata gtacaggaag
      121 tagactaaaa cttttaaaag ttttaagtct atctatttgg caagtttaaa aaataaaaat
      181 tcggtatcaa aaaactgtta ccgataacat ttttgaaagc gttgtctttt gcttctctat
      241 cacttatagt gatgacgtca tattaaaaca caaggaggaa atcaatggat acagctgcaa
      301 tttatcatcg accggaaagt gaatatgcat ttctatatac gaatacaaga tttcgtattc
      361 ggcttcgtac taggaaaaat gatatcaaaa aagtatatgt gctatgtgga gatccatata
      421 ccataacaac tgaaaaatgg tatcaaaaac aacgaccaat gaaaaaatgt ctaagtacca
      481 atgttcatga ttattgggaa attgaagtga cgagcgaaac aagtcgctta cagtatgcgt
      541 tccatgttgt tggggaagat aacacggact gtttttacgg tgatcaagga atttttcctt
      601 atcgagaaaa tgtgattact gaaccaaatt tttattttcg aataccttat tttcatcaaa
      661 tcgatcgatt cactattcca gaatgggtaa aagaaacagt atggtatcaa atttttcccg
      721 aaagatttgc aaacggggac aaagcaaatg accccgaaca cactttacct tggggaagta
      781 aagatccaga ccgagaagac tttttcgggg gagatttgca aggtgtgatg gatcatttgg
      841 attacttgac ggaccttggc atcaatggca tctatttttg tcccatattt aaagccacat
      901 ccaatcataa atacgatacg attgattatt atgaggtgga tcccgctttt ggcgataaaa
      961 aacttttaaa aaacttgatt gatgaagcac ataaaagagg gatacgtatc atgttcgatg
     1021 ctgtattcaa tcatatgggt gtccattcac cacagtggca agacgttctc aaaaacggag
     1081 aaaaatcgat ctataaagac tggttccaca tccgtttttt cccagtagat tcttatcaga
     1141 tgactgactt accagaaact gctgaaaata tcccatatga tacttttgct tttactccat
     1201 tcatgccgaa actaaataca gcaaatccag aagtgcaaaa atatttgctg gatatcgcaa
     1261 cttattggat aaaagaattc gatattgatg gatggcgtct tgatgttgct aatgaagtcg
     1321 atcatcattt ttggaaaaaa ttcagagaag cagttacaga aatcaaacca gacatctata
     1381 tccttggaga gatttggcat tcctctcaag cttggctgca aggggatgaa ttccatgcag
     1441 tgatgaatta tgcatttaca gattcgatta aagattattt tgcaaaaaag aaaatcacag
     1501 cttcacaaat ggtcagtgga atgaatcatc agcaaatgct ttacagagat caggtgaacg
     1561 aaggaacatt caacttatta gattctcatg atactgccag gatcttgacc ctttgccaag
     1621 gtaacaaaga attgatgaag tctgtgatgg cctttatgtt tcttcaaaaa gggtctcctt
     1681 gtatctatta cgggacagaa atagggatga ctggtgaaga tgacccggat tgccgcaaat
     1741 gtatgatatg ggagaaggag gaacaagatc tagagctatt cggttttata aaagaacttg
     1801 tttctctaag gaaacagttg tctaagatta tttctgaagg ttctactcag tggctaatag
     1861 taaatgatcg tgaagataag ctctatttca cacggaaact agagggacag atcatctatg
     1921 tttactttaa tcagtcaaaa gaaccttggg tagtagaaca ggaaaatgag gtgattctta
     1981 gtcaaaattg tcagttgtta gaagacggga aagcagagat acgacaatac ggatttttga
     2041 tcatgtgttg aaaactgtta ccggtaacaa caagaaagcg tttgcaaatg aagatttgtg
     2101 cccattataa taagggtgta acagcacgtt gaatacaaag gaggaaatga agatgaaaaa
     2161 aagaagtgtg aaacagttgg gtttagggtt aatctcttta ggaatcttag gcggattagc
     2221 ggcttgtggc aatggtaact cttcagcaga aggggaagcc gatgaaggaa aaacgttaac
     2281 agtttccgtt gattctggat ataaagatta tataaacgaa ataaaaaaca ctttcgaaaa
     2341 agaaaacgat gtgaaaatca agctagtaga aaaggacatg ttcgatcagt tggaatcatt
     2401 agcgttagat gggcctgctg gaaagggacc tgacgttatg atggcagcat atgaccggat
     2461 cggtgcgtta ggacagcaag gacatctggc ggaagtaaaa ctgggaaatg aagcagctta
     2521 cgatgaaaca gacaaagccc aagtgactta tgatggaaaa atctacggag aacctgcagt
     2581 aatcgagacc cttgttttgt actataacaa agatttagtc gatacagcac catcaacgtt
     2641 taaagatttg gagaatctat ccaaagacag tcgttttgct tttgagtcag aagcaggaaa
     2701 aaatacagga tttttagcta aatggactga cttttattat tcctatggtt taattgcagg
     2761 ttacggtggc tatgtatttg gtgatgatgg cacaaatcct tcagatatcg gtttgaataa
     2821 tgcaggtgct gtagaaggaa tctcttatgc aacagattgg ttcaaaaatg tttggccaaa
     2881 gggaatgcaa gacataaaaa gtgcaggaga cttcgccagc cagcaattca tgtccaataa
     2941 aactgcagca atcattgacg gtccatggca agcgcaaaca tacaaagaaa acaatatcaa
     3001 ttatggtgta gccaaaattc ctactctaaa taatggacag ccttatcaac cgtttggcgg
     3061 aggaaaagga tgggtagtca gcaattatgc taaaaacaaa gacctctctc aaaaatggct
     3121 tgattatgta acgaatcagg aaaatcaaga aaaattcttt gaaatggtaa atgaaatccc
     3181 tgcaaaccaa caagctaggg aaacagcaaa ggctaaaaac gatgaattaa caacagctgt
     3241 gattgaacaa tatgaaacag ctcaagcgat gccaaacatc ccagaaatgg gcgaagtttg
     3301 gacaggtgca gaaaacttga tgttcgatgc agcatcaggt tcaaaaacac caaaagaatc
     3361 cgcagatgaa gcagtaaaaa caatcagtga agccatagaa caaaaatact gattaaaatg
     3421 ataaccgtcc atttttataa aaaatggaac aggtgcttca gataaaagtg gcaagagcga
     3481 gatggaaaat tatcttgctc ttgttgccat tatcttaaaa gcagtttgcg gtttagcaag
     3541 tttagagggg gcattcagat gcagcaagat aaaacaagca atatcaacga aaaaaaaatc
     3601 acgctttgtt cacttattcc agggatgggg cagtttgtaa atggccaagc ttttaaaggt
     3661 atcgtttttt tcgccatatt cgtcctattc attggtcaga tgcttctggg cggatttcat
     3721 gcattggtag gattggttac tttgggaagt ataccaatgg aagatcattc attatttatt
     3781 ttgattgaag gcactttgca attcatcatt acaatcattt tcttgatttt ttacggtttg
     3841 aatcttttag acgcttatcg agtagcgaag atgcgtcaag agggcaaaaa aatcaatcgg
     3901 acgatgaagg aagtcataca taatataggt gatgaaggtt ttccttatct attgacactt
     3961 cccgcttatc ttctcatgat ttttacgatc attttcccag tcctagttac attgttcact
     4021 gcatttacta attatgactt caatcatatt ccaccagcca gtttaattga ttgggtaggg
     4081 gctaaaaact tttttagtat cttcttttta agctcatatc gtaatacttt ttttgcagtc
     4141 ttttcgtgga ccgtgatttg gactttatgt gcaacaactt tgcaaatcac ccttggtatt
     4201 ctcacagcgg ttgttgctca ccagaaattt attaaattca aacgtatttt cggtatcatt
     4261 ttcttattac catgggcggt cccagctttt attactatca tgagtttttc gaatatattc
     4321 aacgatagcg taggtgccat caatactcaa gtgattcctt tcataaacca gcttcctttt
     4381 gtagatatca gtgcaatctc ttggaaaacg gacccgaact ggacgaaagt agcaattatc
     4441 ttagtgcagg gctggttagg atttccctat atctatgtga tggttacagg aatcttacaa
     4501 tcgatttcag aagacttata tgaagcagca aaaatcgacg gagcaaatgc atggcagcgc
     4561 ttttcaaaca tcacgcttcc tgctgtcttc ctagtggcag cgccaacatt tattacacag
     4621 tatacgggga atttcaataa tttctccatg atctatctgt ttaacaatgg cggtcccgga
     4681 agcgtaggcg gaggagctgg ttctactgat attcttattt cctggatcta taaacttacg
     4741 actggaactt ctccgcagta ttctatggct tcagcaatca cgttgattat ctccgtcatc
     4801 gttatctgtg tgtcattgct ggtatttaaa aagacaaatg cgttcaatat gggggattaa
     4861 caatgaaaaa atttcattcg agtgctcgat tccaaaaacg actttctcac ttttttactt
     4921 acttttttct tttaatactg tcagtcatta tcatctatcc gcttttgatc acagcaagta
     4981 ctgccttcaa gtcaggaaat attgcagctt ttagtttaga tctttcaagt gactggactt
     5041 taggaaactt tactcggcta ttcagtgaaa cactttacgg gacttggtat aaaaatacat
     5101 tgatcatttc tgtcctaaca atgatcgtac aagtgactgt agtaactttg gcgggctata
     5161 cttatagtcg ctatcgtttt atcggccgca aatcaagttt gatgttcttt ttagtgattc
     5221 agatggtacc gactatggca gcattgactg ccttttatgt catggctttc ttattagaag
     5281 cgctcgataa gtactggttc ttgacaatga tctatatagg cggtggaatt ccaatgaata
     5341 cttggctgat gaaaggatac tttgacactg taccaataga tttagatgaa tctgctaaac
     5401 ttgatggggc agggcatttc aggatttttt tccagattgt attaccgctt gtcaaaccga
     5461 tgctcgccgt tcaagcatta tgggctttta tgggaccttt tggggatttc atgttagcta
     5521 agttcctgct tcgaacacct gaaaatcaga ctgttgctgt cggcttacaa acatttattt
     5581 ctgatgtgac aaaccaaaga gtctctcttt ttgctgctgg ggcaattttg attgcactac
     5641 cgatttgtct attattcttc tttttacaaa agaactttgt taccggcctg acatctggcg
     5701 gaacaaaagg ataggagcag agtatcatgc aaacatttcc gttaaactat ttttccgcat
     5761 tacgtacgcc aactcaagtg tttgcgggac gcaaactatt atcatggcct aaatttttct
     5821 tgatatttgt atttttagtt tccttgatgg tcatgcctgt gactcttttt tatgccaatc
     5881 agatccaagc gattccatta gaacaatttc tatccgttca ttcgctgatc gatgaacaag
     5941 gaacccaaaa attttctgaa cttgaattat ctgaaactgg gttacaagca tcgcaacaaa
     6001 cgatagctgt gacccctgaa attttggtag gtgtgtcttt atctgagaaa caacaatccg
     6061 atcatggaac ttttattgat tttgagaagg aacaatgggt gatccaacaa aaggataaat
     6121 ctggtatacg ccgctatacg atgaattata gcccatcttt ccaaccagat tctgtacgta
     6181 cgcccgaaga tttccaacga tttttagaaa gagaatttta tgccagtaat cgtccaacaa
     6241 tcattttatc ttatagttta agtcttggtc ttgtcctatt tgtcatgacg agtctcattt
     6301 tattcggggc ttcctttttc ttgtggatga caagaaaaag ccaactttca tccattcata
     6361 cattcaaaga gagtgcaaat ttgatgctga atgttatggg aataggttcc atggcagcag
     6421 cagttgtagg gtttatccac tttgacttta tcttgatgct aagtgtccaa acaacgataa
     6481 ctgtcttgct gcttttatgg gtttttgtca aaacaagatt caaagataag cgtgtcgaat
     6541 gacaagttta agcgatttat gcgacaatga atgtgaaaaa aatgaaaaat aaagtagttt
     6601 ttgaaaaatt tctgaaaaaa ttaaacttct tagttgattc ttttcttttt tagtattaac
     6661 gtaatagggt agttttttta aggaggaaaa tcatgttaga gaaaacttta tacaatcaat
     6721 tattttcacg ttctttctcg ctccctgtcg aagtaaatta ttgggacggt acaacgaaac
     6781 aatacggaga tacggacact cctccccaaa tcaaaatcaa tatccatgaa gaaatcccaa
     6841 tgaaagagat tacaagtaac gcatctttgg cactaggaga agcctatatg gatcatcgaa
     6901 tcgagatcga gggaagtatc caagcattga tcaacgatgc ttatcagcaa gcaaacagct
     6961 ttttacgagg aaaagattat ctaaaatggc tgccaaagaa agaaaaacat acgaaacaaa
     7021 aaaacaaaga agatatccat gcgcattatg acttaggtaa cgacttttat aaactttggc
     7081 tagatccaac tttgacttat tcttgtgctt atttcaatac acctgaagat acgttggaac
     7141 aagcacaaat caataaagtc catcatatct tagacaaact ctttatcaaa gaaggcgaaa
     7201 cgttgcttga tatcggttgt ggctggggaa cattgatgtt cactgctacc aaagagtata
     7261 atgtcaaggc aactgggatc acactaagtg aagaacaata cgatttgatt accaaaagaa
     7321 taaaagaaga acatttagaa gataaatgcc gtgtattatt aatggattac cgcgaactaa
     7381 aaggcgaaac gtttgatcat atcacaagtg taggtatgtt cgaacatgta ggttctgaaa
     7441 atcttgaagg ttatttcaaa gtggtaaaag atttattgaa accaaaagga acagctttga
     7501 tccatggaat cagccgacaa caaggcggtg cgacaaatgc ttggatcaat aaatatattt
     7561 tccctggcgg ttatgtccct ggtgttactg aattagttga taatattaca aaaaacgatt
     7621 tgcaactgat tgatttggaa agcttacgtc gtgactacca attgacatta gaacattgga
     7681 caaagaattt tcatcaagtc caagatcaag tcacgaaaga aatgggtgat tccttctatc
     7741 gaatgtggga tctatattta caagcttgtg cagcttcatt tgaatcaagt aatatcgatg
     7801 tgattcaata tttgcttgtt caacctggaa acaatgacat cccaatgcat cgtgcataat
     7861 tgaggcaaaa gtactgttta aagactattt aaaaagaagc taagttttat gatgctccag
     7921 aagtgaactt ttttgtctaa cttctaggat tgtctctaaa gcttagtt
//
LOCUS       DACCXO010000125         7899 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.130, whole genome shotgun sequence.
ACCESSION   DACCXO010000125 DACCXO010000000
VERSION     DACCXO010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.130"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            30..3308
                     /locus_tag="IVY70_002484"
     CDS             30..3308
                     /locus_tag="IVY70_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP7959590.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3849..5756)
                     /locus_tag="IVY70_002485"
     CDS             complement(3849..5756)
                     /locus_tag="IVY70_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP7959591.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5886..6761
                     /locus_tag="IVY70_002486"
     CDS             5886..6761
                     /locus_tag="IVY70_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP7959592.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6841..7815
                     /locus_tag="IVY70_002487"
     CDS             6841..7815
                     /locus_tag="IVY70_002487"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP7959593.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
BASE COUNT         2526 a         1473 c         1674 g         2226 t
ORIGIN      
        1 ctttctaatt tgttacggga ggaatacact tgttaaaaaa acgtataaaa tacagtgcgc
       61 taatgatgaa tctccttatg ctcagtaccc cggttttaat ttctgtcgtt gaagttgctg
      121 ctaacgaaca acagtctgtg aacgaagaaa atgcacacgt gaacattgaa gggatgtcaa
      181 caatggagga aataccaaga actagtgaag atatggatac ggaagaagga aatgatccta
      241 tgatgaaaga atcagaaaat gaaaagacaa aaaagcaatc tgaagaagca aatgaagagt
      301 ggaaaggggt tgttttcgga gaaagcacaa gtgctagtag gtatgccatt gaaccattag
      361 aagacggcgt gcggctctct tccacgaata acggcggaaa attccagtca agcggatcag
      421 atggaatgac ctattattat caagcaatcc ccaaagacat caactttagg atgcgggcga
      481 cgatcgaaat agaatcttgg acctatacga atgcccaaga aggatttgct ttaatggtaa
      541 gagacgcagt accaaaaaat catttgtttg ggcaggcatt ttattcaaat tcatttgcca
      601 tactaggcag tcggatcgaa tatgtctggg actcggatag acaagaagtg gtcaacacga
      661 gcggcagcaa atatacgatg cgacttggat tggggacacg ggcaatcaca ggaatatcat
      721 ctgaagatcc gcaagctgcg ccagctcctg ggagtgtctc agtggaaaca acacctttag
      781 agacttcagc agggttcttg gaaaaagaaa caggttctta taatatattc ggaaacggtc
      841 atgggaatct gtttccggcc acgaactcga ttactaaact ggacgtagag atgaaaaaaa
      901 cgaatactgg tttagaggca tattattacg atcctgaaac aggagaagaa atggcttctg
      961 acatcatgta tgactgggaa aaattatacg cttcagatga atcggttatt tatgcaggat
     1021 ttgctgcagc aaggaatatg acgatcaagg tagaaggaat cgaagttgct tactcagatc
     1081 ctgcgacaga tcctcctggg caagaaagac ctactcgctt gattgatccg atttacactg
     1141 tgacctctag taatcattca ggaaaccaag atcatactat gtcctttaga gcaaatgctg
     1201 acggattatt gacaattaaa aataaagcaa caggagaggt gcttaagaac gcaaaaaact
     1261 tggctatcac tgcgaatcgg gaatttgatt atcagacaaa actgatggaa ggaacgaacg
     1321 aatttacttt cagttttacg ccagataaaa actatccgcc agaagaatac gtagagatga
     1381 ctagttatga aacaaaagag attcttcata cagtggacta ccgtaaacct aaatattcga
     1441 cagtttatgt aacaccagaa gggtcagatg caggaaacgg aagcagacaa aatccattat
     1501 ctctgacaca agcattgaga tacgcatatg ctgggcagac aatcgtcatg gcgcctggaa
     1561 cttattcctt cgaaagagga ttgacgattc caaaaggcgt taacgggaca agagaaaacc
     1621 cgattcagtt gattgcagag aaaaatccag aaaacaagcg tcctgtactt gattttaaag
     1681 gaactggaaa tgggatgaca ctgttcagtc actactgggg tttacgaggg tttgacatta
     1741 caaatagtgc agctatgcaa aaaggattac agatttctgg aaatcataat ttgatcgaag
     1801 agattcatgc ttatcggaat ggaaatacag gaatccaaat cagcggtagc tcaaatgatc
     1861 ctttcgaagc atggcccgcg cataacttag tcaagaactg tacatctttt gaaaatgccg
     1921 atccaggatt tgaagatgcc gatggatttg cggccaaact gacaatagga gaaggaaatg
     1981 tatttgacgg ttgtatcgcg tatcataatg cagatgacgg ttgggacctt tttgctaaaa
     2041 atgaaacagg ttcgatcgga aaagtaatca tcaaaaactc agttgcttat cgaaacggct
     2101 gggtaccagg aattgaggga gaaggtaacg gtaatggatt caaaatgggc ggttcttctt
     2161 taacgggccc tcatgaattg atcaatagtt tgtctttcga aaatcttgcg aaaggaatcg
     2221 attccaatag cggaacagat attatcgtca atagcagtac aagttttaat aacggttcat
     2281 ataatgtggc tctttatacc tcttcagcag gaaacacaga ctaccatgct tctggtatct
     2341 tatctttccg aacagagaat cttgagatga gagagcagat ccgcccattg aatcaaaacg
     2401 aagatcaaat ctaccactcg ttgaactact attggaatga acgagagaaa caatcagaaa
     2461 atactcttgg acataccata tcaaaagatt ggatcgagtc tctaaataca tgttcgaaag
     2521 aaggtcagca gcctgttacg cgctatgaaa atggaagtat ccatgttcat ggactgatgc
     2581 agattaaacc ggggaatcgt caaacagaaa aggaaagagt ggatggtctt ccattatctg
     2641 taggtgctat ttttgacgga gaaacctcac cgtcttcgga atatccagag tatgcaatcg
     2701 tacaagaacc aaatcagaca aaaccaaatg aacaaaataa tccgattatt atacaagaag
     2761 caaatggtgt ggcggagaat gcagaaggaa atatcattga actgaagcct tttacagttt
     2821 ttgtgtcaac tttcacacca aatcaaggaa cgcctgtctt tgaagatatg cgttatttcc
     2881 cagaaaagat ccaaatcgtt gaaacacagt caagtgctca attttctaat cataccgcta
     2941 ttttttcagc accttcaaaa ggagaatata cgctagaagt cacttatcgt tcggaaatat
     3001 ttgatgggga aaaatggtct gctcatacag aatctgttac ccagcaaaaa gcgatcactg
     3061 tgcgagaaac tgccgatgtg cccccgggaa atgacaaacc agaaagagaa aatccaggaa
     3121 atgaatcacc aaatggaaaa ccaaatggaa aaccttctca tccggatagg acagcaaaag
     3181 aaagtgaaaa aaatggcact caaggactcc caaaaacggg agaatttaat aacccgttgc
     3241 tagcattagc tggaggaatt ttgttaatcg gtgtagttgt ttatgtcatg aaacagcgaa
     3301 aaaaataagt ggaatttctc cttttatgat gtaaaacaat tgactgagga aagtaaaaat
     3361 agaaggaaat gagaccaata gatgtctcac ttccttctat ctttttatag atgaggttct
     3421 ccttgctgct tttgattggc tgttcttatt acttgatgca ggacagcttt ttcacaatct
     3481 ctccactaaa cggtgttcaa tcagctgacc ctcggtatta gctcaacaaa cggcaaaata
     3541 tgaggaacta tttttccgtt tttttctcta atactcaggt caggacgctg atttcacaac
     3601 ctctcgataa acggtgttca aaagccagtt tctcggtagt aagtcaaatt tatgcaaaaa
     3661 tgtggaaagc cattttcaca aaatttttct tactacttga aactaagcag ctttttcaca
     3721 acctctaata catggtattc cagaagtaac ttcttcgaaa aataagccga aatgtcacaa
     3781 aaatttgaga agcgattttc ccaaattcct tcttatttct tgaagctgac cacttctggc
     3841 acaacctctt accagattcg cactcttttt tctggcgcta gatacatagc gtcttgttcc
     3901 gtgataccaa atgtttcata gaattcgtca aggttttttg gttgcacgtt tgctcggagt
     3961 ttagcagggg catggacatc gatttgcagt aaaagttgtt ggtattcttt ttttgctttt
     4021 gtacgccaaa tcgttgccca attgacaaag aattcctcga tagaaagatc tgattccgtt
     4081 tttgctgctt ccaatgcaca gcttagaccg cctgcatcag caatattttc cgaaacggtt
     4141 aattttccgt ttacttttcc gtcagcaaaa ggcagaccat cgaattcttc gatcattgct
     4201 tgtgctttgt tttgaaattg tgtaagatct tcttctgtcc accagttgtt caagttgcca
     4261 tattcgtcaa ataaagcgcc gttattatca aatgcatggg aaatctcatg agcgataaca
     4321 gctccgattc caccataatt agcagagctt gattgttcta gactgtaaaa tggtgcttga
     4381 aggatcgctg ccggaaagac gatcacattc cgaaatggat gataataagc attgaccgtg
     4441 tcagcactca tttcccattc tgaacgatcc actggtttgt tccatttgct gaaacgatct
     4501 tttaatgtga gtttgctgaa aacaagagta tttgaaagca aagtaccacc ctgatctgca
     4561 ggtactgttt tgaactggtc gaataatgct gggatcttat cggggtagcc tacttggata
     4621 cctaagttat ttaattttat gacggctttt tcttttgttg catccccaag ccagtcattt
     4681 tcttctagac gttttttata aacggcgatc atttttttca ccatttgttc tacgtctttt
     4741 ttagcagctt cgccaaaata tttttttccg tagtaatcgc caactacttg atcgaattgt
     4801 ccagaggcaa ggtaatacgc tgcttttttc tgaggcattg cttcgtctgt tccagacaat
     4861 acgcgagaat agattccgct gacttggcgg aattcttctg atagataacc actaagagaa
     4921 cgtactgttt taatgatcat ccagcttttg aataacggaa aatgagcttc tgtcaggatg
     4981 tcattcaaat gatcgaagta cactggatca gttacgatca ctttatctgg agttgtccca
     5041 atcaaacccg tgacaagttt tttcaagtcc agtttatctg tgtatttaat aaatgtatca
     5101 aaagattgcg gattatacat tttgctgtaa tctgcgtttt cttctgcact ttttacatga
     5161 ggagcaatca atttatcaaa ttgcactgct tgttccgtga ttgcttctgc ctgacttttt
     5221 tctttgccgg caagctctaa taactgaaca gccatatcaa agaaaacgcc tattagttgt
     5281 gcaccgttgg gatggtctga tgcataatac gtcttatctg gaaggaataa agaaggcgga
     5341 taagcaaaga aggcgttcgt ttgcgcattc ttcatatctg catctacatc cagattaaat
     5401 ggcaatggta atgtatccaa cagccattct ggaaattgct tattcaattc gtcgaaagac
     5461 tgcaatgatt caactttttc gattataggt tgaagcggtt gagcatgcaa ctcatttctt
     5521 cggtcataat cgtttgccag ttgataatat gcaaggaaat gtttcatttt ttctgaagac
     5581 actttttctg gatgggcaag cagatcgtct gtttctccca tcaaaagttg gtcgatcccg
     5641 tcaactaaat cttggaaacc gcctgtagct ggtttatctg cagggatttt tgctgttttt
     5701 tgccattctt cattgactgc ttcatagaaa tcttgtttca atcgttcttt gttcattcat
     5761 cgatcgctcc ttttttgttt cgtgtgccca ttatcttaca aaatgaagga attaacaaat
     5821 agaacataaa aaattgaaaa ttcgaacaat caaactgatt tgtttttttc aatccttcac
     5881 ttataatgaa aaaaaggagg cgatcccaac tgaatcaagt agtggataaa cctttatatt
     5941 tcaaagtaga taaaaaaatt aaaaaattag ccagcactca gcagctgcag tcaagaaaaa
     6001 gtaaattgct ttttttagct ctggtttttg aagatcaaag ttatgtgatt atcgatcaat
     6061 caggacatcc ggtagaatat tctccagcaa aatatactta tcaagagggt ctttcttgta
     6121 aaaaatggaa gctgatcaat gaagagccaa tcgagttatc tagatggatc aacagaaaag
     6181 aagatattcc cgttttgatc gaagaaaaaa ggtcaggaaa ggaactcgca aattgctggg
     6241 tgggattgcc agaagaacgt tttttgaggt ataagaaatg ggctaccccc tcaggctatc
     6301 tttgtggtac ttacgcggca gcagttctcc ttgcctatta tcaagactat cgtaaggggt
     6361 ggatgcttcc aaatgagatt cgcaagaaaa atacggctga ttcgttggtt ttgacgaagg
     6421 cattgcgtag ccagatccaa ccactaggac ttcctacgat tccttttcag gttagtacag
     6481 ggatttctag ttttttaaag aagaatggca atcatgagcg tgcacgagca actcttctag
     6541 gttcatggca gcgagctaca aaacgtatac gtgaaggcaa acctgtgatg attggtattt
     6601 tgaaagtact tggcagtaca tatggcaatc attgggtgac agcgtacgct tattttgaaa
     6661 ctgaaacagg agaacgttat tacaaagtac acgacaactg gggcgattat cataaagtca
     6721 ttcctgccag ttggagtaac ggaacagttt cacttcctta agaatttgct gaaattaaca
     6781 gaaaatcttg gaaaaatatg ttaaactatc gaagaataga gaagttttgg ggaggactac
     6841 atgagtaata agtatcagga tgacacgttg aagattttta gtttgaatgg caaccgtcca
     6901 ttagctgaaa aaatcgcgaa agtgttcgga acagaattag gtaaaagcgt cgtgaagcaa
     6961 tttagtgatg gcgagatttc catcaatatc gaagaaagta ttcgagggga tcatgtgtat
     7021 atcgttcaat caacgaatgc acctgtcaat gattactata tggaactact gatcatgatc
     7081 gatgcaatga agcgagcaag tgcgaaaacg atcaatgtcg tattgcctta ctatgggtat
     7141 gctaggcaag accgcacggc gaaacctcat gaaccgatca ctgcgaaatt gatcgctaac
     7201 ttgatcgaag aagcaggggc aactcgagta ctgactttag atctgcacac ggttcaagta
     7261 caaggattct ttgatattcc tgtagataat ttatttacga tgccgttgtt tgctcattat
     7321 taccgtcaat taggtctgac gggagatgac atcgtagttg tttcgccaaa aaacagcggt
     7381 gttcagcgtg cacgtagtct ttcagaatat ttgaacagca cattagccat cgtagatcat
     7441 gcagatgatg cttcaactga gggtggttat gtgatcggtg atgttcaagg aaagacatgt
     7501 atcatggtag atgatatctt gaacacagga gcaacgcttg cacgagcagc aaatgtatta
     7561 aaagaaaacg gcgcaaaaga agtatatgcc tgtgcgtcac atggattatt gtccgatcat
     7621 gcgaaaagta ttttagacaa tgcaccaatc aaagatattt gtatcactga ttctgttttc
     7681 acagaagaaa atcgtcatcc agaaaactta acaatcgtta catgttcaga attaatgggt
     7741 gaagcggtca aacgcatcca tgaaaataca ccgatgagtc cgttattccg tctggaagaa
     7801 aaagaattcg aataaacaaa aaaaagaggg aaagaagttg tgacagaagt gagcagcttc
     7861 aaggagctgc gacaagactt ttgtcacagc tcctttttt
//
LOCUS       DACCXO010000126         7680 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.132, whole genome shotgun sequence.
ACCESSION   DACCXO010000126 DACCXO010000000
VERSION     DACCXO010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.132"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            28..2355
                     /locus_tag="IVY70_002488"
     CDS             28..2355
                     /locus_tag="IVY70_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7959594.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2481..2810
                     /locus_tag="IVY70_002489"
     CDS             2481..2810
                     /locus_tag="IVY70_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP7959595.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2850..3854
                     /locus_tag="IVY70_002490"
     CDS             2850..3854
                     /locus_tag="IVY70_002490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7959596.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3851..5280
                     /locus_tag="IVY70_002491"
                     /pseudo
     CDS             3851..5280
                     /locus_tag="IVY70_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            5486..6658
                     /locus_tag="IVY70_002492"
     CDS             5486..6658
                     /locus_tag="IVY70_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7959597.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNKAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6820..7275)
                     /locus_tag="IVY70_002493"
     CDS             complement(6820..7275)
                     /locus_tag="IVY70_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959598.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
BASE COUNT         2594 a         1442 c         1528 g         2116 t
ORIGIN      
        1 tttgatgatt ttttattgag aggagggatg aaaatgatta agaaaactca aaataaaatc
       61 caacgtatat gtcaaaaaca ttttgtttct agactcaaag ctgtatctaa aagaaaatac
      121 ttacatccca ttatgatttc gatcatcttg atttttactt tcttagggag cttatcaagt
      181 atttatgtaa acgtttatac agaaacgtca aattttgcta accaaacgac agaggagaca
      241 aatagtccac cctattctga aatacagtcc atagaaagca ccaatcagtc tagcaacaac
      301 gagacaacct caaccacatc ctcgactcaa ctgattgatc cgacagaaaa agaaatcaca
      361 gcagcaaaag cagcagggga gaaagcttat tcagaaacag gtagaagcca gacatttagt
      421 actgttgcgg ccactgcgaa tttcaacgga cataccatac cagatggata tacagtgata
      481 tatgccaaag atggagtcat tgttgcggtt aacggatcga tgagtgcgga taaaactgcg
      541 ttacaaggcg taaccaaagc aatcgctttt attgataacc gtccagatat ccataatctg
      601 tttattggct ccacagtaaa tggtactact atcacattcc caaaaagtat ctatatgggg
      661 gtggctaccg ctggaaatac gatcgattca gttacaccca atgcggataa aagttattct
      721 tttacaccct caacatgggg agggcttcct gccggaggag gtatgagtac tttttcaatc
      781 ggtatacagt tagcaaatga tattccattc acggggtact cgatcaaaaa tgggcagatc
      841 gttgatggta agggaaatgt ttcaaatttt ccaacctatc aagcagcaac ggttcaggtc
      901 aatgcgaatc ggacaggaac tttttcttat acagtcaatg gtggagcagc aaaaatcgga
      961 acgtacggga ctacattatc tggaatccat gtgggtgaca aagtggttat cactccaaca
     1021 gcagtaacgg gctattcttt tgcacaaaca aacggttctt tcacagccct agaaagtaac
     1081 gtcgatacgg tgacctatac aggcaaacaa acaccaatca ctattaagta tcaagactat
     1141 tttggacaga tgattgctcc ttctaaaata atgaatctta cttacggaag tgctgcacaa
     1201 gacctgacga ctaatacgcc tactatcaac ggatatacct ttacagctgt ttcagcgaca
     1261 gaaacaaaga accaatcagc aatgagtgtt ttagctagtt tagatacaaa tggaaatgta
     1321 atcgtaaagg acgcaaatgg aaaacagatt tctgaagtca tcctatatta taagacaaat
     1381 gcaacagtat caatcaatgc aaatggcagt aagtactatg atggtctttc tgttgtaccg
     1441 atcgttaaat atgcctttaa tgataaaaca gaatcaacat cccttttaaa caatgatctc
     1501 agtattgatc aaatcacatg gaatccaact gatttcaaag cgatgaatga aaagggagtt
     1561 gaagtaactg cgcctactga aattggaacc tatacgtctt ggcaattgac aaaaagcgga
     1621 ctagctaaac tagctgccag aacaaactat ttatttacta tcgtgcagac atctgatgtc
     1681 tatacgatta agcaaatcag cggtgcggta gagctcagtg gcagtaagac atatgatgga
     1741 aaagcaggtg tcccgtcaat ccatgtcaag ctagcagagg gtgtcactag ttcgcttact
     1801 ccagtacaag tggcacttag cagtatggat tacacagcag atgcacaatc agcaaaaaat
     1861 atggttaatg ctggcagcta tacaattaaa ctaacgaata gcggaataga caaagtcaaa
     1921 gcagcaaatc ccaagttgtc atttactgat atagccaaca catcaggaac ttataccatc
     1981 tataaagcta acgcagtgat tactgtcgat gacgctgcct ttaattatga tgcccaaagt
     2041 cactcgattt cggttggaaa tctccacgtt accggtgttg tcttaggcga atcattggat
     2101 tataccctaa cagttaacag ccggacagat attggatcac aaatagtaac gattagttta
     2161 ggcgaaggaa gtgtaaacaa taattatacg attatcgcaa cagataccgc taagttgacg
     2221 atcaatccga ctctcttgct tccttcgaca ggtgggatag gtctactgcc ctttgtcttt
     2281 ttaggactga tctttatttt ttctggcttc ttttatttta tccatcgtaa gaaaaaagca
     2341 ggtgaacagc gatgaaaaaa acaaaaaatg tacgtgtgga tatatttgct gttctctttt
     2401 ttatcctact ggtctttttt ataggttctc cgactgtgtc agctagagaa tcacctatta
     2461 catttacttt gcataatcga atgttcgaaa gcgataagat gccaagtatc agtcaaaacg
     2521 acggaataaa tatcccggat aactttcaag gagtaaaagg gttgaacggc gttactttcg
     2581 ctatatatga tgtctcggat gaattttaca agctgcggtc agaaggaagt agtgtggaag
     2641 acgcacaaag aaaactagca caaaaaagtg atagtgaaaa aatactggca gaaaatgtga
     2701 caaaaacagt agacgaggaa gagggcattg cttcttttag tgcatcggac aaagatgagc
     2761 aaggtcgtga cgctgtttac cgatttgcgg aaataaagac ttcagattaa gtaaaagaaa
     2821 aatcagcacc atttgtcgtt gtgcttcctg tgacagattc atctaatcat aaattgacga
     2881 ccatccactt gtatccaaag agcgaacaaa aaatacctgc tgcactcaca ttaacaaaaa
     2941 cagtagagaa taagcagaca gattttgcag atggggataa agttccttat ctcatcaccg
     3001 taacaattcc tgagaacatc aacgagatag gaacctatac gatcaaagat acagctgatc
     3061 ctcagttatg gctagaacca gaaacgatcg attttttgat tggtggagac aagattcata
     3121 catttcacac acaaaaaaca aaacatggtt ttgtcttatc agattctgga aaagatctta
     3181 gttcgtttgc tgggaaaaaa ttaacgatcc gttaccaaat gacattgaaa gaaaacagtg
     3241 aaaaaacgac atttgataac gatgtacgtt tgacgactga caatcaaaca gtagaaacac
     3301 atcttgccat ccaaacagga ggcaaacaat ttgtcaaagt ggatcggcaa acgaaacaaa
     3361 aactagcaga tgctcaattt ctagtcaaaa aaaatgacca atacttagcg caagaaaacg
     3421 ggataaatca ttgggtagcc aaagatgatt caagagcaac gatttttact tccagtaaag
     3481 atggaacatt ttctgtccat ggattgtctt ttggaagtta cgaactagtg gagattaaac
     3541 cgcctaaagg atatatattg agtcaatcaa ccattctctt tgaagttgaa aaaggcagtt
     3601 atgatccgct tcatcctata ccattggaaa tcatcaacga accaaaagca aatccgacga
     3661 ataatggaaa aacgccagtt cagatgcaga caaatggtcc atcaaatgaa aaaaatggag
     3721 gaagctttcc aaaaacaagc gaagagatgc ttggaggttt ctcaatttta gggttgttgc
     3781 tagtcctttc aacagggaca gcttggtttt ataaaaaaca acaaaaagga aacaatagga
     3841 gggaaataaa atgaaaaaca tcaaaacaaa aatatggacg tttttaggaa ctgctatcat
     3901 gctgcttcct ttcgttctcg gcctaggaac tgcagaggtt tctaggccgt atcacctaca
     3961 ccagaaaatg ttacggttaa tttgcataaa ttgaaattca cttcagcacc agaaaatcaa
     4021 ataaacaacg gaacggaatt gacatttccg aatagcgaac cgttgaacgg cgttgaattc
     4081 aacgtatatg acatcactgc tacgtactat ccaagtaaag acacagcagt tccagcagac
     4141 gctacaccct tcgcaagtgt aacgacttct ggtgaaggat tagctaacct gactcttcca
     4201 ggtaaatctg atgggaaaga tgctgtatac gtctttgtag aaacaccaaa gcccggtgtc
     4261 gaaacgtcgc caaatatcgt gttaagctta cccttttata acccgaattc atcagaccct
     4321 gacaaacctt tagacacgat tcacctatat gctaaaaatg tcatcaaaga aagcaatatc
     4381 aaaatccaga aagctgggaa cttttcaggg gtagataagt tagcaggggc gcaattccag
     4441 atcaaaaatt cagagaatca attcgtgacc ggttttgatg aaaacactgg tgtcgctctc
     4501 tatgatcctg ctgagtcagc gttgacattc acaactgata ctgaaggatc catcagtgtc
     4561 aatggattaa tagacggcaa ttatacttta gttgaaacaa aagcaccaga tggttatcaa
     4621 ttaaccaatc cacaaaaaga aactactttt atagtaaaaa acggaaaatt gcaacaaaca
     4681 accggtatca tccatggcgc agatgaagcc aatcttggga atccaaacgg tgtagtcaat
     4741 cgggaaaata caactacgga taacacgatt actgtagaaa atgtccaaaa ctacggtgat
     4801 ttccacttca tcaaacaaga tgtgaacacg caagaaaaat tagccgatgc agaattttct
     4861 attctaagag caaaagatcc aactgttaaa ttaatgaaaa aaatagcaaa tcttgcctca
     4921 ggagaatacc catatgcttg gtcagatgaa gtcacaggtt ctgactggga agaagtgaaa
     4981 ttgatttctg attcagaagg gaaattttca atcgaagggc tgaaacaagg ggcatactgc
     5041 cttcaagaaa caaaagcacc agaaggctac gcattaccta agaatgaagc tgcgtacact
     5101 gaatttacag tagatgatga tccaacaact tctgatgaaa caacgatcga taaccaacct
     5161 aaaggaatct tgccttcaac tggtggtatg gggatcatcg tattcatctt ggtaggtact
     5221 gctttagttg gaggagcagt gatctacttc aaaaaacgcc atcaagaaac agaagcttaa
     5281 actattggaa acaaaaatca tacgataaat aaacgatagt gattcaaatg gggagaaagg
     5341 ttgtgacaag tggtcggtct cttggtattc gctcaacaaa cggcaaaata ggaggaacta
     5401 ttttgccgtt tgttgagcga atactcaggt caaggcgctg atttcacaac ctcttcatat
     5461 acatattaga aaataggtgg acatgatgaa taaaagcaga ataagaatac tggacatctt
     5521 catggcaatc attttagtcg ttgggatcgg gatcttttct tatccttttg tagaagattc
     5581 actgaatgat tttcttgccc agcaaatgat catccattat caaaagcaag catcgaataa
     5641 gaattcagag gaaataaaaa aacagcagga aaaaatgaca aagaaaaacc aacagttggc
     5701 agagcaaaac gtatcaccag ggataggcag tttcaacaaa gcagttgatg caaaagcatt
     5761 aaaagattta cctagtaatg ccttttttat ggaacatatg cttggagtca tcgaaatccc
     5821 aaaaatcaat gtcagcttac ctatattcga tcagacaacg gaaatatttt tgcaaaaagg
     5881 aacgtcatta ttggaagaaa gttcttatcc gactggcggg aaaaacaccc atgccgttct
     5941 ttctgggcat cgtggactcc cagaagcaaa attatttaca gatttgccta agttgaaaaa
     6001 aggagaccag tttttcatcc agatcaatgg aaagacattg gcgtatcaag tagaaaaaat
     6061 ccaagtggtc ttacctgatg aagtagattc tttgggtatc caaaaaggca gagatctcgt
     6121 gactttgctt acctgtactc cttatatggt caatacacat cgactgcttg tgacggggca
     6181 tcgtattcct tatcaagcaa aagaggcaaa aaaagcgatc caaggaattg atcaatggaa
     6241 aaaatggaaa ttttttattt ggtttatcgg tatactactt ggatcaatcg gcttagtttg
     6301 gctactcatc gcttatttag attctttggc catcgctaag cgaaactatc ctttatcttt
     6361 ttatgtaaag aacacaaacg gacgaccaat cgaaggaatg gtcttttctg taaaaacgct
     6421 caacggcaaa cactatatta ctagagaaaa agttccattt gtgaaagctt ctgatgaata
     6481 cggactagtg agattttctg atttaaaagg caggaactat cgcttgcaac atgaggagct
     6541 cctgcttaaa atccatgtaa aacataaaca ttcgaaacag ttttcaatga aattgaaaaa
     6601 aggaagatat aaactgcgaa aagaaaaaga ggtgtactat ttgattgaga aagaatgaat
     6661 gaagatgtcc aaaaaagcag agggaacaga ctaaatgaaa aaagagagaa gctgacgaaa
     6721 tggacgtgta aactgctatt tttggtttac tatctggaac gccagcttct atttttttat
     6781 gtttcagaag aattttatta ttatagtata acttttgaat tagtttccat aacatttgga
     6841 gcaaggggtt aaaccacgag cttgagcttc gcttaaagtg gcaggggtat acgtaccatt
     6901 tccacattta tgtgtatggt attttgaacc ggtcggtgtg acatagacag tttgttcatt
     6961 attttgctca ggagctgcgg cttgtgcctg ctgagaggcc tgttcagctg ctgcttgctg
     7021 tcgcgcctgt tctgcttgtt gctgtgcgat tgcttcttgt tgcgcttgtt ctgcttgttg
     7081 tcgtgcaatt tcttcttgct ttttccgttc ttcttctgct tcttttgctt tgattgtgga
     7141 atcaattgca gcaagacggc ttgtcaactc ttgtttgttt tcaggtagcg cttggatagc
     7201 tgctgaagca gttgcatagt tttcttttgt aggatttgct tcagcttgtg acaaagcaga
     7261 ttccgcttct gtcacttttt tttctagtgc agctttttcc gctttttgtt tctcttcttc
     7321 ttttttcttt tcaatttgtt cttgttcttt cgatgaatct gccgtacttt cggagatcgt
     7381 ggtactgctg ctggaagcct gagctgaact tgtgctttga gtagtagacg tagtagcacc
     7441 acacccactc aacaaacttc ctgcgaccaa aaacaatgca actaattttt tcactaaatt
     7501 tcctccttaa gtaaaaaaca accgttttca ataaatgtcc ttttcaattg tttacttact
     7561 tgttataata attgtgttat gaatagtgat tgaggaaact taatctaatc ccgttttgat
     7621 acacgggatt ttttgctata gttctgttat taagtatgca ataataaaat atgtacgtta
//
LOCUS       DACCXO010000127         7625 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.133, whole genome shotgun sequence.
ACCESSION   DACCXO010000127 DACCXO010000000
VERSION     DACCXO010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.133"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(14..706)
                     /locus_tag="IVY70_002494"
     CDS             complement(14..706)
                     /locus_tag="IVY70_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7959599.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IVY70_002495"
                     /pseudo
     CDS             791..967
                     /locus_tag="IVY70_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IVY70_002496"
     CDS             complement(1143..1712)
                     /locus_tag="IVY70_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7959600.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IVY70_002497"
     CDS             complement(1786..2109)
                     /locus_tag="IVY70_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959601.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="IVY70_002498"
     CDS             complement(2263..3075)
                     /locus_tag="IVY70_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959602.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="IVY70_002499"
     CDS             3429..4277
                     /locus_tag="IVY70_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP7959603.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="IVY70_002500"
     CDS             4422..5363
                     /locus_tag="IVY70_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP7959604.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="IVY70_002501"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="IVY70_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1512 c         1371 g         2470 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaaa
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag ttacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgagaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgaa tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagctttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaacgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tcgaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacct
//
LOCUS       DACCXO010000128         7454 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.134, whole genome shotgun sequence.
ACCESSION   DACCXO010000128 DACCXO010000000
VERSION     DACCXO010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.134"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            188..418
                     /locus_tag="IVY70_002502"
     CDS             188..418
                     /locus_tag="IVY70_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP7959605.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            515..1816
                     /gene="murA"
                     /locus_tag="IVY70_002503"
     CDS             515..1816
                     /gene="murA"
                     /locus_tag="IVY70_002503"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP7959606.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1829..2005
                     /locus_tag="IVY70_002504"
     CDS             1829..2005
                     /locus_tag="IVY70_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP7959607.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2140..2436
                     /gene="yidD"
                     /locus_tag="IVY70_002505"
     CDS             2140..2436
                     /gene="yidD"
                     /locus_tag="IVY70_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP7959608.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2621..3088
                     /locus_tag="IVY70_002506"
     CDS             2621..3088
                     /locus_tag="IVY70_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7959609.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3173..4057
                     /locus_tag="IVY70_002507"
     CDS             3173..4057
                     /locus_tag="IVY70_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7959610.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4174..4407
                     /locus_tag="IVY70_002508"
     CDS             4174..4407
                     /locus_tag="IVY70_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP7959611.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4495..4575
                     /locus_tag="IVY70_002509"
     tRNA            4495..4575
                     /locus_tag="IVY70_002509"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4529..4531,aa:Tyr,seq:gta)
     gene            4582..4653
                     /locus_tag="IVY70_002510"
     tRNA            4582..4653
                     /locus_tag="IVY70_002510"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4614..4616,aa:Gln,seq:ttg)
     gene            5168..5875
                     /locus_tag="IVY70_002511"
     CDS             5168..5875
                     /locus_tag="IVY70_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7959612.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5934..6578)
                     /locus_tag="IVY70_002512"
     CDS             complement(5934..6578)
                     /locus_tag="IVY70_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP7959613.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6704..6991
                     /locus_tag="IVY70_002513"
     CDS             6704..6991
                     /locus_tag="IVY70_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959614.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7099..7419
                     /locus_tag="IVY70_002514"
     CDS             7099..7419
                     /locus_tag="IVY70_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7959615.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
BASE COUNT         2470 a         1201 c         1609 g         2174 t
ORIGIN      
        1 tttggaataa gggactgcga caagactttt gtctcagtcc ctttttttgc atatttttag
       61 catttatttc ctaaaaaatg taaggggatt cctatatttt taagtatact tgtttttcct
      121 tttatacatt ttatggtatt atcattttat tagttattat aaaacaatcg ttagaaagga
      181 cgtcattatg caagtatacg gaattgatgc ggtgcttcga atcgtgagtc atttggcgtt
      241 tatctactta gccttctggt ctttacgttc gttacgcatt gaaacttttt tcagaccaat
      301 gcataatacg caaattagaa tggcaatcgt acttttttca atttttttag ggtacactgc
      361 aagtaatttt ttcttagaag ttatcgcttt atgccgaaat cttttcgtta gcctgtaaat
      421 tttaaaaaaa ggtactattt taaaagcaag gatgttataa tagaaaagat tttgagtaag
      481 tgtatgctca ttggaatttt ttggagggaa caacatggaa gagatcatcg taagaggtgg
      541 caatcaatta aatggaacag taagaattga aggagctaaa aatgctgttt tgcctatttt
      601 agctgctagt ttgttagcag aagaaggtat tacgacatta gacaacgttc cgattctttc
      661 tgatgtcttc actatgaatc aagtgattcg tcacttaaac gtagatgtag attttgatga
      721 acaaaagaat caagtaacaa tcgacgcttc aagacaatta gaaatcgaag cgccttatga
      781 gtatgttagt caaatgcgtg cttcaatcgt tgtgatgggt ccgctacttg ccagaaatgg
      841 acatgcaaaa gtagctatgc ctggtggatg tgcgattggt aaacgaccaa tcgatttgca
      901 tttaaaagga tttcaagctt taggagcaaa aattattcaa aaaaacggct atatcgaagc
      961 aattgcggat gaattgatcg gtaacacgat ttatttagat ttccctagtg taggtgctac
     1021 tcaaaatatc atgatggctg ccgtaaaagc aaaaggaaca accatcattg aaaacgtagc
     1081 tcgcgaacca gaaatcgttg atttagcaaa cattttgaat aaaatgggcg ctcaagtcta
     1141 tggagctggt actgaaacaa tgcgtattga aggcgtggat catttacatg cagtcaacca
     1201 ctcaatcgta caagatcgta tcgaagcagg tacctttatg gttgctgctg caatgacaca
     1261 aggcaatgtg ctgattgcag atgctatctc tgaacataac cgcccattga tttctaaatt
     1321 gatcgaaatg ggtgcagaaa tcattgaaga agaaggcgga gtccgtgtca tcggaccaaa
     1381 acatatctta cctacagatg taaaaacgat gcctcatcct ggtttcccta cagatatgca
     1441 agctcaaatg acagctatcc aattagttgc agaaggtaca agtgtagtga ctgaaacagt
     1501 gtttgaaaat cgtttccaac atttagaaga gatgcgccgt atgaacgctc atgtcaaaat
     1561 cgacggaaat gtagcaatca tggatggaaa ccatgaattg caaggtgcag aagtatatgc
     1621 aacagacttg cgggcagcag cagcattagt tttagctggc ttgaaagcaa atggaatcac
     1681 acgtgtgcgt aacttgaatt atctagaccg tggatattat aatttccata tcaaactaca
     1741 gcaattaggt gcagacgttg aacgtgtaga catggatcaa acttctgctg aaaaaacagc
     1801 acaaacgatt gcttaaggaa gtggctgaat gagtaaccaa cgatatatct tattaacttt
     1861 gataaagatt ttaatggtga tcttattgct tatccttctt tttgtggccg gaacgatgat
     1921 cggatatggt gtcatcggtg gcggcaatcc atttaaagta tttcagccaa gtttgtggat
     1981 tcatattcga gacttttttc attgataaat aagaatcaca gtggttctag tcgatatgaa
     2041 gtgagccatg aagcagaaca tgataaaaga aaacagaagg gaacgttgac gttagtcgcg
     2101 atcccttttt ttagtaaaag aaaagaagga atctaattga tgaagaagat attgatttat
     2161 ttagttagag gatatcagcg ctttatttcg cctgcatttc cacctagctg ccgttattac
     2221 ccaacctgtt ccaattatat gataaaagca attcaaaccc atggcgcgct aaaagggacg
     2281 gtaatgggga ctgcacggat cttacgctgt catccttttg tcaaaggcgg tatcgattat
     2341 gtaccattga cattccgatt aacgcgaaat aagaaagata aaaaagaacc agattataaa
     2401 aaggttgtga aaaatacggg tagcactgag cgataaagaa acaatttgga aaatagcttc
     2461 ttatattttg caaaattgtg agttattgcc gaagtgttgg attttgaaca ccgtcaaaaa
     2521 aaaggttgtg aaaaatacgg ctagcactga gcgataaaga aaaagaggaa atttattgtc
     2581 aaagtaacaa ctccgcaact acttatatgg gggacaaaaa atgatcgaag aactaaacaa
     2641 gaaagattta ccatggtcct tgttattaga tgcagatcct gataaagaaa aagttcaagt
     2701 atatgtttcc agagggagtg gctggatatg gaaagaaaaa gaaaaaacaa taggtgtact
     2761 gatctatgtg gcaagggaaa aagaatacga aatcgtcaat gtagcagtaa ccccaacgta
     2821 tcaaggaaag gggattggcg gaaaattact tgagacagct tttcagaagc tttcacaact
     2881 cgcttccagt caaactcaga tcatcatacg aaccggcagt actagcagtg cggcgttaca
     2941 tctctatcaa aaaatgggct atgtggagat tggtcgagtc aaagactatt ttatccatca
     3001 ttactctgag cccatttttg aaaatggaga tcaattaagg gatcaagtga ttttagcaat
     3061 tcacttacct aaaaatagct ttaactaata aaatttcaat tcaagaaaat gctttcaaat
     3121 gctaaataga tacattacaa tagctttagc aaacaaaaag gagaatattt ttatgggaat
     3181 attagttttt gattttggtg gatcggctgt caaatatggt tgttgggatg gcaaagagat
     3241 taaaggtaag ggacaatttg caacgcctga atcttgggaa gaaatgaaag cgcaattatt
     3301 tcaagtttat aaaaaaatac tcgtttcttt tgaagtcgaa ggaattggaa tcagctctcc
     3361 tggagtagtg aataatgaga aacaggtgat tgagggaatc agtgcgattc cttatattca
     3421 tggatttaat attttccgag atttggaaac tctgtttcaa cttccagtaa cgattgaaaa
     3481 tgacgccaac tgtgccggaa tggctgaatt ttatgaaggt gctgcccgcg attatcaaga
     3541 tgttgctttt gttgtggcag gaactgggat tggcggggcg ttgttcacga aaggacaaat
     3601 caataaaggg gcacatcttt acggtggaga atttgggttg atgttcttaa atggcgataa
     3661 gacattcagt aaacttggta cagcggtaca gatggcttgg cgttattgtg aacgcaaagg
     3721 tctcgataaa aatgcttata ccggaaaaga tgtctttgaa ttagcagaaa ctggagatgc
     3781 aatagcaaaa gaagaagttg agagttttta tacgtatctt acaaagggat tattcagtat
     3841 tcaattttcg tttgatccag aagtgatcgt attaggcggc ggggtttctg caaaagaagg
     3901 gctcattgat gagatcaaaa gccgaatgaa gaagctgaca gaacaattcg atctgcatga
     3961 tttcgaaccg cagattttat tatgtgaata cagaaacgat gctaatcttg taggtgcagc
     4021 agcaaactat ctcgctgtac gtcaagggga gaattaagag ctttagagaa ggtgcagctt
     4081 gcataaaaga agtgaatgaa atgaatagga gatttttacc taaggaatgt tttatttgct
     4141 gcagtatgat aaaatatctg agaggtgaaa atgatgtcaa aaaaaaataa agatattgaa
     4201 gtgcgtatag aagaagtgaa aaaagatatc aaaggtaaga attatgaagt cactcagtta
     4261 tttataggga aaaaaatgat tggtgagatc ttagcctatg gaccaaaaga atatgaaatt
     4321 ttctttggtg aagaagattt tggaaaagaa aaaagtcttg aaaatgcgat agaaacagtg
     4381 attcgacact ggaatctgca cgaataaaaa aatatcaaaa aaactttcaa taaagggttg
     4441 caaaaatctg gaaaattagg tattataact aagtcaggtt gatacaaacc tttcggagga
     4501 gtagcgaagt ggctaaacgc gacggactgt aaatccgttc cttagggttc agtggttcga
     4561 atccactctc ctccatttca ttggggtata gccaagcggt aaggcaaggg actttgactc
     4621 cctcatgcgt tggttcgaat ccagctaccc cagtatacca caatcgtaag ctctcgttag
     4681 ttcgagagct cttttttttg ctttgctacc ttgttccttg aagattctgc tgatttaaga
     4741 tagagttaag ttaactacaa gaattggagg gatccacatg aaatggaaag aaaaagtaga
     4801 cgaagcagct gaaaagttat atgatttggt caaatcagaa aaatacaata ttgaagtgaa
     4861 tattccgaaa aaaggcggaa aagcggtccg ggtaaaatca aaacgcccaa cgaatcatac
     4921 aaaaaaatgg atggctaaaa accgctaata ataacaaaag atccataagt cggcattttc
     4981 atcgatttta gggtcttttg ttattttcta ggttggacat atctaaaaaa ttgtttctga
     5041 caataggagt aggaagaacg atgtgtgtct aaatcctctg ttgatggata gaaaccttta
     5101 tctttttcag ttgtgaatca ggtatatacc agttataata gaagaaacga aaggacgggt
     5161 gtacgttatg tcgaatcaat tacctgtata tatacagatt catgaccaat taaaaaatga
     5221 aatcgaacaa ggtatatgga aagtcggaga ccgtcttcct tcagaaagag aattagcagt
     5281 gaaattcaat gtcagccgta tgacgttgag acaagcaatt caaaacttgg cagatgaagg
     5341 aatcttagaa agaaagatcg gttcgggtac atatgttgca cgagaaaaag tacaagaaaa
     5401 aatgtctggt gcaacaagtt ttacagaaat tatggaatca caaaatcgag taccgtctag
     5461 tcggacaatc tcttatttct tgacttcgcc aagctcaagt gaaatggaaa aactaaactt
     5521 gagtaaagat gacacgatct tgcgaatgga acgtattcgt ttcgcagatg atgtccctat
     5581 ttgttttgaa gttgccagtg tgccacaaaa gctgatcgat ggctacagca aagcagagat
     5641 tactgaatcg ttgtatcgca cgctagaaga aaaaggcggg aaaaagatcg gtccagcgaa
     5701 tcaaacgatt actgctgtgt tggcttctga gaaaattgct gactatttag aggtaaaacg
     5761 aggagatgca gttcttcgtt tgcgccagat ttcttatttt gaagacggta cgccgtttga
     5821 gtatgtgcgt acgcaatacg taggaaatcg ttttgagttt tatttggaaa aataaaaaac
     5881 aggtgatccc tgattccgta ttagaaataa aagagaatca gaatcacctg tttttatata
     5941 tgatcagtta ccagttgctc gtgataagaa acaggaccct cttcaaattg gacttgtccg
     6001 ttcagcagct caatgatgga ttctttaaag tggtcagcac ttttttcatc tacagcacag
     6061 atgacgctaa cttgatcagt atattggatt tcttttataa aaaaatgttg gagttcaaga
     6121 taattctgta attttcctag ttgcggatac gtgatcatca cagagatctc ctgttgcaag
     6181 gtaccttcta cgattccaat gtcagacaat gcttgagaga ccgcatgagt gtatgctcgt
     6241 attaatccgc ctgtgccgag tttcgttcct ccaaaatatc gagtgactac tgcagctaca
     6301 ttgatcagtt gatttttttt gaggacttcc aacatcggta tgcccgccgt tccacttggt
     6361 tctccatcat cgctgcttcg ctggatttca tttttttctc caatgatgaa ggcgctgcag
     6421 ttatggttcg ctttccaatg ttcttttttt atttcttgaa tgaaactttt tgcttcgtcc
     6481 tctgattcta ttcgtctaag agaacagata aagcgtgatt ttttgatctc gatctcactt
     6541 tgtccatttt cttttattgt acgatagcgc tcgatcatct tgtcacctct tttttcattt
     6601 tctttattca gtataaataa agttctaggt tgtttcaaca ctgtaacggt tgcgcaattg
     6661 ctgtagtatg cgataataaa gacaaaggca ggtggatctg gggatgcagg aacgaatgaa
     6721 aaagtacgat gcaattacgc attatttgaa gaacaatggc ggctctcaag taacgttgac
     6781 ctttacccaa tttgacgaac tgctgtttcc cagtaacgga cttcccaaaa cagcaaggga
     6841 aagtacagat tggtgggcaa atgattataa acaccctgaa aaaggtgcct atggctggat
     6901 aaatgcaggc tatgaagtag ttgtcatcaa tttggacaaa gaatatgtcg tgttcaataa
     6961 attagtgaaa tctagttggc tatttgatta aataaaccgc gtttagaaaa cgttgacaaa
     7021 tttctttctt tttatataat agagtaacag acgcgaagaa aacgtttgcg tagtttgttt
     7081 taaaaaggag agaaaagtat ggctaaaaaa acaatcatgc ttgtatgttc ggctggtatg
     7141 agcacaagtt tactagtaac aaaaatgcaa aaagctgctg aagcaaaagg aatcgactct
     7201 gatattttcg ctgtgtccgc ttctgacgca gataacaatc ttgcaaataa agacgtggat
     7261 gtgttgttat taggcccaca agttcgcttt atgaaagctg attttgagaa acgtctggaa
     7321 ccaaaaggca ttcctttaga tgtcatcaat atggctgatt atggtatgat gaatggcgaa
     7381 aaagtgttag atcaagcaat cacgctcata ggtgaataaa tagaacagta taactatcga
     7441 acaagcgatc caaa
//
LOCUS       DACCXO010000129         7052 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.136, whole genome shotgun sequence.
ACCESSION   DACCXO010000129 DACCXO010000000
VERSION     DACCXO010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.136"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..104
                     /locus_tag="IVY70_002515"
     CDS             <1..104
                     /locus_tag="IVY70_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP7959616.1"
                     /translation="FQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(172..465)
                     /locus_tag="IVY70_002516"
     CDS             complement(172..465)
                     /locus_tag="IVY70_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP7959617.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(493..1158)
                     /locus_tag="IVY70_002517"
     CDS             complement(493..1158)
                     /locus_tag="IVY70_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP7959618.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1298..1930)
                     /locus_tag="IVY70_002518"
     CDS             complement(1298..1930)
                     /locus_tag="IVY70_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7959619.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1937..3004)
                     /locus_tag="IVY70_002519"
     CDS             complement(1937..3004)
                     /locus_tag="IVY70_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7959620.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            complement(3001..3732)
                     /locus_tag="IVY70_002520"
     CDS             complement(3001..3732)
                     /locus_tag="IVY70_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP7959621.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(3900..4379)
                     /gene="greA"
                     /locus_tag="IVY70_002521"
     CDS             complement(3900..4379)
                     /gene="greA"
                     /locus_tag="IVY70_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP7959622.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4515..5690)
                     /gene="mltG"
                     /locus_tag="IVY70_002522"
     CDS             complement(4515..5690)
                     /gene="mltG"
                     /locus_tag="IVY70_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP7959623.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            5855..7015
                     /locus_tag="IVY70_002523"
     CDS             5855..7015
                     /locus_tag="IVY70_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP7959624.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
BASE COUNT         2088 a         1487 c         1106 g         2371 t
ORIGIN      
        1 attttcaaaa gaaattaatg gctatttttt taaccaatct gaaaagctac tttgccaccc
       61 atgagcttca aaccaatcaa gtaaaattgt ctgcaggata ttaatagctg aaaacaatat
      121 tttcaaaaga aaaaagagaa ggtgacaaaa attgtgtcac cctctctttt tttattgttt
      181 tttaaagtga taggttggct cctccaggtt gggatcataa tcaacaagta aatcatatcc
      241 tttaaagaac cattcatctg cttcctcgat aaaaaaaagt gtcccatcca tttccacttt
      301 ctttacagga tgttctggct gttcaactga cattccaatc gaaaaaccat catgaacctc
      361 tgttttgccg tagacttttc caaaaaaacg tattccgtaa gtttctggca gatcaatttc
      421 tgagtcaaac cacttttgtg cttttggcgt taccgttaat ttcatcccaa caacctactt
      481 tctcttttct ttctattgat tcatcttatc gtctagaata tccacatcgc ctgtctctcc
      541 aataaccagt agattatcat tttcacgaac atattcatct gctgcaggag aagcgataac
      601 cgtttgatct actcttcgta tggcgactac tgttaagtca aaacgttgcc tgaaatttaa
      661 ttcagctaat gttttgttga aaaatttagg attggagact cggatttctg ctaaagaata
      721 tttatctgac agctctaagt agtctagaat attctttgat actaagttat gtgcaatccg
      781 tatccccatg tctcgctcag ggtgtacgac acgatctgct ccgatcttct ctagtacacg
      841 ggcatggtac tcattttggg cttttgctaa aatattcggt acgcccattt cctttaccat
      901 cagagtaact aagatgctgg cttggatatc ttctccaata gcgacaatta catgatcgaa
      961 atttcttata cctaatgaac gaagtgtcat ctcatcctgt gcatttgcca caacagcatg
     1021 ggtggctata ttcatatatt cattgactcg atcttcatta ctgtcaatag ccagtacttc
     1081 ttgtccagct tcgatcagtg tacggcagat gcttccgcca aatcgtccaa ggcctacaat
     1141 tgcaaaattt tgtttcacca ttttcctcct ttagtatctt cttttctcag tataacaaag
     1201 cctttccaaa aacaaaagca agcatcatta ttgtaaaaaa gccaagaaaa ataatagtta
     1261 ttttctcagc ttttgattat cctctttatc atcgttttta tttcaccaaa ccatgtttaa
     1321 acgcatagat agctgcttga gttcggtctt ctacctctaa cttagctaat atattcgaca
     1381 catgcgtttt gactgttttc agtgtgataa acaattcatc cgcgatttcc tgattacttt
     1441 tcccttcaga aatcagcatc aaaatttcat tttctcgatt cgttaattct tcatgcaata
     1501 ctggatcatt tcgccgactc attttttcca tcatttttgt tgtcacttca ggttctagta
     1561 ctcgctcgcc gcgttgcgta gcccttatag catcagctat ttcatgtgct gtagacgtct
     1621 tcaacaaata tcctgctgct cctgcttcaa ttgcaggata aactttttca tcatcaataa
     1681 aacttgtgac gataatgatt ttggcttttg gccaatcttt taagattgct tttgttgatt
     1741 cgatgccatc catttcttcc atgactagat ccattaaaat gacatccggt cgaagtgcca
     1801 tcgccttttc atagccttga cgtccatttt cagcttctcc aatcacttca atgtcttctt
     1861 ggatagataa ataagaagag acgcctaagc gtaccatttc atggtcatct actaataaaa
     1921 cttttatcat ctttatctat gcctccttca tcaaagggac cttggtctcc acgctcgtac
     1981 cttgcccttt aaaactaatg atttttactg ttccaccaat accagctact cgttctttga
     2041 tattgttcaa tccataactt cccgttttgg tttcattcat attaaatcct gtcccgtcgt
     2101 caatgatacg taataaaaga ttgttatcta ttttatgcaa atagacttct aattcattag
     2161 ccttagcatg tcttaatgtg tttgataaca attcttgaac gattcggaac aagtgatcct
     2221 caatggaact agttaatttt acatcttcta cctcccattt tagcgaaatc tggattttat
     2281 tctgcaattc cttcaataac tgctcaatgc cttgttttaa acttttttct tctaaattga
     2341 ctggacgcag atgaagaagc agcgcacgca tctcagactg ggaagcgttg atgatctctg
     2401 ccaccatttt caactgctta cggaacattt ctggtgtctc acttttttct gcttgctccg
     2461 tcaatgccga catcatcatc atcgctgcaa ataattgctg tgaaactgaa tcatgaagct
     2521 cacgagctaa tcgatgcctt tctaatttta aaatctcttc tttggtttgt ccatctacat
     2581 attgaggacg gctagtcata atctgtaatt cactggatac ttcgatcatt ttttctttta
     2641 ttttagtaat ttccatatca atatctttga tgtacagatc atccgacgcg cttgggatcg
     2701 gctgatgcaa aagtttggat tcataattgc cagaagaaag tgcacgaagc ttttcttcga
     2761 ttttcccgta ttttgccttt tgaataagtg ataacaatag aaatacgatc aatcctacac
     2821 ctaatgaaat tgccatcaaa tggaagataa gcggaacgta taataaacgc gcacgcagca
     2881 attctcccca ccaatgactt tggttactgg cataaaaata agtgaaaagt gaaaatagaa
     2941 taataaggaa agccgcaatc cccgaataaa cagctagcat tgctttggat attcttccca
     3001 tcatattcgt atcacctcga catctccaac caaagtattg gtaatgatct tcaatctgcg
     3061 aggattttca tcataatcac tgctgcagat tttgatctgt tcgtttttta aggaaaactg
     3121 ctcttcttca aacaagacat ttcctactaa tgtcgcatgc tctaaacgaa tagccactcc
     3181 caggggtact aagatccgtg ttctgcctat cccttttctt acaatcacaa tattatcatc
     3241 ttttggcaaa agtgtattgc ccaaatcgat gatagtatca ccagaaatga tggcaatgtt
     3301 gatatcatcc cattcataga cgtcgtttcc aatacgttgg ttaccaaata attgctgtct
     3361 tttacgctca ttattatgtg tttttaattg ctctgtttgg accatcatga tctgtttttt
     3421 tcgccaaaaa gcattttttg tcaaatctat tcctgaaatc tctacacctt tcagcccgat
     3481 aaataaaacg gccaatacaa ccatcatcca taaagcagga ctgttgacta agctaaaaaa
     3541 aatgaccaag ctccctaaga tcaattgaaa attttgaaat ttagtacgag gatatttgcg
     3601 catagctaga tatatattga aaataccgaa tattactaaa agcaaaagtc cagtattgtt
     3661 tacgatttgc caaacagcaa ataacagcag cagtgcttcg actaccacaa aaaaacgcca
     3721 agaactattc atcgcttttt ctccctcctg acacattctc atttcatcat acctattttt
     3781 ttgactgttg gctataggac tttagatgta agatcgttca gtgaaacctg caatactcaa
     3841 agagaaaaga tctagagtgc gtcatcagta agatgaactc tctagatctt tcagtcagac
     3901 tacgcttgtt ctactttgac gattttaact aacatatcgc cacctggtgt agcaatggtt
     3961 acttcatcat ttttacgttt gccaatcaat gcttgcgcga taggagaatc attggaaatc
     4021 tttccagaaa acgggtctgc ttcagcactt ccgacaatcg tgtactcttc ttcatctcca
     4081 tcaggcaact cgatgaacgt aactgtttta ccgatcgata cctcatctgc atctacgccg
     4141 tcattatcga tgatttgtgc aaaacggatc atattttcaa ttgttgtgat tcttccttca
     4201 acaaatgctt gttcatcttt agctgattcg tattcggagt tttcggaaag atctccgaaa
     4261 ccgcgagcga ttttgattcg ttcaatgatt tctccgcgtt taactgtttt tagttcttct
     4321 aattcttttt ctaatttttc tttaccttct agagtcattg gataaacttt ctctaccatg
     4381 tgtaacgtct ccttttttcg ataaaatacc gcaaaaggta atcttttgag gaatatagtc
     4441 caaaaaagat taccacgctt ttcttaaaat gtaaatgttt ttctaaaaat gatcaatgac
     4501 tagcccctgt ttttctaaga attatttaca tatttttgca ctagttccat atgttcttca
     4561 tacgttttag cgaaataaac atttccagtg ctcgtatctg ctacgaaata ataatagtca
     4621 ttttctgctg gttctaatac tgccttgatt gcctcttcgc ttggattgtc aaaaggacca
     4681 ggcccatagc cggtattggt atacaaattg tatggtgaat ctacagctgt atcttcatac
     4741 gtcaccaatt ctttatgttc tcctaatgca tataaaacag agatatctga ctgaagtggc
     4801 atatttgcct tgattcggtt aaagaagact tgtgcaatat ttttacgatc attttctttt
     4861 acgccttctt tttcaactag agaagccaaa gttaaaactt cttgtacagt caaatttttc
     4921 tgtttgatag ctgaataata gttttgcatg accgtatttg ttttgttcac catctcaatc
     4981 acaagatcct taagcgaagt actcgtgtag taatcataag tagctgggaa taagtagcct
     5041 tccaaacggt attttgtgtt ctcagcttta gaggcacttt ccaacaattc tgggaatttt
     5101 tgatacaact cgttaaagaa agtctcatca tttactaaat ctaaaaattc tttcttgtct
     5161 ttaccagtta ccttcgctac ccgctcagca atctgatcga tgtcgtagcc ttctggaatg
     5221 gcaattttcc catctgcaac ttttgttggc tcagatgtgc cgccttcttg caactgcttg
     5281 ccaatctcat caagagtcat attaggcgct aactgataat aaccagcttg aaagccagtt
     5341 aagtttttaa attttgtata ataattgaaa acaatcccac ttttaataat attgtctttc
     5401 tccaaaattt cgccaatctg cttattagaa gagccgcttg ggatttccac ttggactaat
     5461 tggtcgtccg atttatctaa aggcttcaac ccgctatcta cataacgata tacggtaaac
     5521 ccgagaaatc cgccaataat caataatgta agagcgatca ctaaaatgat cttcctcacg
     5581 atcttatctt cttttttacg cagatttctc cgcttgtcga ttatatctgc tgtagctgcc
     5641 ttactggctt cttttttgtc tttgcttggg tgttgtttgt ttcctgacaa aacaactcct
     5701 ccttactatc atcttcgtaa ctctgtttat tatacatgaa atttctactg ccgtgaatta
     5761 ttttaccaaa tattatgtca aaattgtaag tggcaaatgc tctttttgag gattacaatg
     5821 aaaaatagtt aaaaatggat aggaaggatt taatatggca aaaggaaaac atcaaaaaaa
     5881 acagcagatc tctctttctt ttctcgcagt tttgttgctg gctgtcagct tcttttcaac
     5941 aggcatcatt acaggatggc aattaacaaa acaacctcaa ccacaaattt ataaaacgga
     6001 acccgcacaa cttgcttcca atttaacatc taatgagcag atgcttgcca gcaaaattga
     6061 tcaattactg caaaaatcag attatatcgg ttcgatttat gtacgcaaaa ataatcgagt
     6121 gatattagaa aaaggatatg ggtacgcaaa tctacgcata aaagaatcga attctccttc
     6181 tctttactat caaatcggtt cgatccaaaa agcgatgact gctttactta tcctaaaaca
     6241 aattcaatct ggcaagataa cttttgacac aaagctatct gaattttatc ctcagattcc
     6301 tggaagtcag caaatctcaa tcaaaaatat gctttacatg cgctctggtc ttcatcgaac
     6361 cgcttctcca aaaaaaccta tgtcagatag tgagatcatt cagtttgccc ttcatcattt
     6421 gaaatttact ggttatgata cgtatcatta tgaaccatta aattatacat tgcttacagg
     6481 ggtattgatc aaactaatga atcagcctta tgaaactctt ttaaaaaaag aaatcattcg
     6541 gccactccat ctagaacata cagattttta tgaaaatgta aaaaattccc ctcagcatgc
     6601 tgtatcttac caaatgtctg caactgacga ttattcaaaa gcattggtcg agcctgaaac
     6661 agacatccga aatgaattag gcacaggaaa tataagtatg tctgtttatg acttgaacaa
     6721 attctttacc agtgttctgt ctggcgatat tataccaaaa gaactacttt tctcattgtg
     6781 gcaaaataat ggtgataaac atccttacag cggcggtgtt tacagtggaa atgattatat
     6841 ccttgctcag ggaaatatca atcgattcca tgcagtagcg gcgatgaaaa aagacacaaa
     6901 agatgcaatc gtcatggaaa gtaatatcca atctgataaa aacatcaaat tgccggccac
     6961 tgatttaaga aatcagattt acgaactgat ggaaggtgtt aatttaaaaa gctaagtcga
     7021 gacaaaaaaa ccagcaggat gctggtttca ga
//
LOCUS       DACCXO010000130         6881 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.137, whole genome shotgun sequence.
ACCESSION   DACCXO010000130 DACCXO010000000
VERSION     DACCXO010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.137"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            389..1519
                     /locus_tag="IVY70_002524"
     CDS             389..1519
                     /locus_tag="IVY70_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP7959625.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            1611..2366
                     /locus_tag="IVY70_002525"
     CDS             1611..2366
                     /locus_tag="IVY70_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP7959626.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            2424..4463
                     /locus_tag="IVY70_002526"
     CDS             2424..4463
                     /locus_tag="IVY70_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP7959627.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4572..5723)
                     /locus_tag="IVY70_002527"
     CDS             complement(4572..5723)
                     /locus_tag="IVY70_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP7959628.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(5877..6407)
                     /locus_tag="IVY70_002528"
     CDS             complement(5877..6407)
                     /locus_tag="IVY70_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959629.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(6506..6856)
                     /locus_tag="IVY70_002529"
     CDS             complement(6506..6856)
                     /locus_tag="IVY70_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP7959630.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT         2363 a         1251 c         1330 g         1937 t
ORIGIN      
        1 cactatttca tttcaaattc cattgcctaa tcattaggaa aagtctatca tgaactcgta
       61 aatagaatat agtaataacc ttgttaggtg aggctcctat acaaacatag gctgttgccc
      121 agaaacgtcg aaagtcgcca atgggtaaaa cagaaactgt tggattaagg ctttttccaa
      181 tagctaagaa tgttttcttt acgttgtata gtgctaaaac tcggacgatg agatgatgct
      241 aggtcagaat tcttgttcga aaaggtttct gaccttttct ttatattttt gtgactgact
      301 tatttgatga gacaaactcg ctcctcacct gacaagtgga ttgctgtgtg aggaggagat
      361 gcggtgaaaa aagatcgatt ggtggatttt gggtccttat ttgactttgt ccatgatcgg
      421 tcttttagaa gtatacagtg ccagttctta tcgtttattg caagccgatg aaaatacaaa
      481 aagtctatta ttgcgtcaac taattttcat atttttgagt tggagcgtga tcttcttagc
      541 tcgttcagtc aaactacact atttacttca ccctaaaata gcaggatacg gtttagcctt
      601 atcgattttc tttttagtat tagtaagaat agggatattc ggtgtcactg tcaacggcgc
      661 acaacgttgg atctctctgt ttggcattca attccagcct tctgaactgg caaatctttt
      721 tttgattttt tatttaagct ggttttttcg tgacggaaat agtagcccaa aagatctaaa
      781 aaaaccattc ctgattacag taggtataac ttttctgatt ttatttcagc caaagattgc
      841 tggagcattg atgatccttt cgattgcgtg ggtcatattt tgggcagcgg cggttccatt
      901 taaaaaaggg atctatctaa tcgttacttt ttctgcattg ctgattggag cagcaggcgg
      961 ggtattatat ttaggaaata aaggttggct tccacagatg tttaatcatg cttatgaaag
     1021 gatcgcgact ttaagagatt cttttatcga tagtcatggt gcgggctatc aaatgaccca
     1081 ttcgttttat gcactatata atggtggcat atttgggcgg ggattaggaa atagtatcac
     1141 aaaaaaaggg tatcttccag agacagaaac agattttatt ttttcgatta ttacagaaga
     1201 gcttgggcta atcggtgcat tgtgtgtgct tttcttattg ttctcattat gcatgagaat
     1261 cttttgttta agcagtcgct gtaaaaatca gcaagctgga ttgtttcttt taggattcgg
     1321 aactttgcta ttcgttcaga cgataatgaa cgtgggaagt atcgcggggc tgatgccaat
     1381 gactggtgtg ccattgccat ttgttagcta cggaggaaca agttatctga tcctttcttt
     1441 aggaatcgga atcacgttga atatttcttc aaagatacag gccgaagagt tggcgctcta
     1501 tcgacctgaa aaacaataaa gagaaactga gaaactctca gtttgaatta tcataaagta
     1561 caatctttta tttttcaagc gagaggttct tttagaaaga ggaattcgca atgaaagtat
     1621 ctcaaaaaat catattatct attttgttac tgcttacatt agtagttggc tatttttcga
     1681 ttgagtttat ccatggattt tcgtctgcaa aacagagttc aactgtgaaa aaggtagagc
     1741 cgaaaagtat ccctaccaca ctaaatgtgg ctttgattgg ttcagatgct cgttcgaaag
     1801 acgaaaacgg tcgctcggat tcacttctgg ttgcacaata caaccagaaa acacaacaag
     1861 caaaactgat ctctatcatg agagattcat atgtcgatat tccaggttac ggaatgaata
     1921 agataaatgc agcttactct tatgggggag ttgatttatt aaatcaaaca ttaaaggaaa
     1981 atttcaaatt tgaagctccc tattatgcaa gtatcacatt tcaagatttt atcgattgcg
     2041 ttaatgaact gtttcctggt ggagtcaaga tcgatgcaga aaaatctcta gatttagatg
     2101 gcgtatatat aaacaaagga gagcaagtga tggatggtaa tactttactc cagtatgctc
     2161 gatttcggga agacaaagaa ggagacttcg ggaggatcag aaggcagcag caagtgatca
     2221 aagctatctc tcagcagctg aaagacgtaa cttcaatatt caagctaccc aaagcggttg
     2281 ggaaattact tggtagtatc caaacgaacc ttccagaaag tgtgcttctg gattgtggta
     2341 tggatttcct caaagacata tgttagaata aacaggtata aatagtgaaa taaaggaatg
     2401 acaagcaaga gaaggaggaa aaaatgaaaa gaagtgacaa gcacggcaaa aatcgaacag
     2461 gcgcttatat tgccggcgca gtgatcttaa tagcagctgc gggtggcggt tatttttact
     2521 accagcacta tcaagaaacc caagcagtag aagctggaga aaagacagtt gagcaatttg
     2581 tccaagcttt gaacaaggga gattataaca aagctgcaga aatgacatca aaaaaggcgg
     2641 caaataaaag tgcattatct gaaaaagaga tcttagataa ataccagaat atatacggtg
     2701 ctgctgatgt caaaggactt cagatatcga atctaaaagt agataaaaaa gacgattcta
     2761 cttacagttt ttcatataaa gcaaagatga atacctcatt aggtgaattg aaagatcttt
     2821 cttataaagg aacattagac agaaatgatg gtcaaaccac gatcaactgg caacccaact
     2881 tggtttttcc agaaatggaa ggaaatgaca aagtaagcct gaccacacaa gaagcagcaa
     2941 gagggaatat tatagatcga aatggtgaac cattagcaac aacaggcaaa ctaaaacaat
     3001 taggagtcgt tccaagtaaa cttggagatg gaggcgaaaa aacagccaat atcaaagcga
     3061 ttgcttcctc cttcgactta acagaagatg ctatcaatca ggcgatttca caaagctggg
     3121 tacaacccga ttactttgtc ccattgaaaa tcattgatgg agcaacgcca gaacttccag
     3181 ctggagctac cattcaagaa gtagacggca gatattatcc tttgggtgaa gcagctgctc
     3241 agctgattgg ttacgtggga gatatcacag cagaagatat tgataagaat ccagaattaa
     3301 gcagtaatgg aaaaatcggt cgctcaggtt tggagatggc ttttgataag gatcttcgtg
     3361 ggacaacagg tgggaaatta agcattacag atgcagacgg tgtagagaaa aaagttctga
     3421 tcgaacatga agttcaaaat ggcaaagata tcaaattgac aatcgatgcg aaggcacaaa
     3481 aaacagcttt tgacagtcta ggaggaaaag ctggatcaac tgttgcgaca acgccaaaaa
     3541 ccggtgatct tcttgcgctt gctagctctc caagctatga tccaaacaaa atgacaaacg
     3601 ggatctcaca agaagattac aaagcttatg aagaaaatcc tgaacatcca ttcatcagcc
     3661 gatttgcgac aggttatgct cctggatcta cgtttaaaat gattacagca gcaatcggtc
     3721 tcgacaacgg cactatcgat ccaaatgaag tgttgacgat caacgggctt aaatggcaaa
     3781 aagacagttc ttggggatct tatcaagcaa cgcgtgtcag tagtgatgta tcacaagtag
     3841 acttaaaaac tgctttgatc tattccgata atatatatgc ggcacaagaa acgttgaaaa
     3901 tgggtgagaa aaaatttcgt acaggcttag ataaattcat ttttggtgaa gaccttgatt
     3961 tgccaattag tatgaatcca gcacaaattt ctaatgaaga tagctttaac tcagatatct
     4021 tgctagctga tactggatat ggacagggcg aacttctaat tactcctatc cagcaagcag
     4081 caatgtattc tgtttttacc aacaatggca cacttgtcta tcctaaattg attgcagata
     4141 aagagacaaa agataagaag aatgtcatca gcgaaacagc agtacaaacg atcgtgccag
     4201 atctgagaga agttgtgcaa gatgtaaatg gtacagcaca ttctctttct gctttaggga
     4261 ttccgttggc agcgaaaact ggtacagcgg aaatcaaaga aaaacaggat gtaaaggggc
     4321 aagagaacag tttcttgttt gctttcaatc ttgataacca aggttatatg atggtcagca
     4381 tgttggaaaa taaagaagat gatgattcag caaccaaacg agcatcggaa ctattgcaat
     4441 acctcaacca aaattatcaa taaaaaattg ctttttaagt acagtaaaag gactgtggcg
     4501 ttatcgtcac agtccttttt tcaaataaac ggttttcacg atgtctcttt cacaacttct
     4561 ttttgattta ctcacacttc ataaactttt tttgtaaaat atttgagaac aagtggagaa
     4621 ataagtgtac ttaataaaat cacgataact aaaggtgaat agtactgtgg ttcgatcaat
     4681 tgactttgct gtccaatttg taaaatgatc aaagccattt ctccacgcga aatcatacca
     4741 gcaccgacca tcaatgaact atgttgagaa aaaccggcaa tctgagcacc aaaataacct
     4801 ccaagcaatt tggtcaaaat cgctacaagc gtcaatatag aaataaagag tagttgttca
     4861 ctaaagtcag aaaaatcaac ttctaatcct acactgacaa aaaatactgg tataaatact
     4921 gcatatccta aagcctctac attgtggtat acttcttctt taacctttgt ctgactgaca
     4981 gctatcccag caaaaaaagc accaatgaca gagctaagcc caacaagatc cgctacgtaa
     5041 gacattccta aacaaataac tagcgatacg atgataatag aagaatttgc gtacactttt
     5101 tctgcaaatg aaagtaggaa aggcgcaatc catttgacta ataagaaaat aaaaacaaaa
     5161 taaattagct gttcaatcaa aacaagcggt aaagaagcat tgctagaagc ttcccccgtt
     5221 agaaaagata ggctgaaact tagaactaaa acgactaaaa tgtcatctac cacagaagca
     5281 cccaaaattg ttgatccttc ttttgtattc actacgttta gttcttttaa tacttctact
     5341 gaaatactga cagaagttgc cgcaagaata atcccaaaga aaatcgcttc atttcccgaa
     5401 acttggaatg ctttgcctgt caaccagcca aaaacaaccg ggaagatgat cccaagcaaa
     5461 gcaacgaaca taccaggtcg gaaatatttt ttcaaaagag ataaatcact ttccagacca
     5521 gctaaaaaca ttaataagat cacaccaatc tccgaaaagt catggactag aatatcaggt
     5581 ttgacccaat tcaatccagc ttggcctagc aaaacaccaa caagtaattg tccaatcact
     5641 gctgggatac ctaatctccg cgagatatgt gagccaatcg ttgttgaaat caatatcagg
     5701 cataaaatac caataaattc cattctcatc attcctttgt ataaaatata gcccgaactt
     5761 agcataacat acattattga aaaaatagga aaatatactc acaaaaaaac caacaatcca
     5821 aaagattttt ttggattgtt ggtttttata cggtctattt taataactaa aaatgattaa
     5881 cgtatagtca tttttcttat tctgatgaaa ttgataatct cctggatgcc catgaaaatc
     5941 aaaataaagc cgaatatttg gaatagaagc atcaaacttt tgaatggatt gaacaataag
     6001 acgaatccgc caatcaacaa cagaatacta ctataaacac tccaactttt agcttttgta
     6061 ttcatgctga agaacaactg gaacaagctg ttgatgataa cagctaatcc aagaagaatc
     6121 ggcaggatag aaacgattgc aggtgcaaaa gcatagacga tcagagctaa gatcaggaaa
     6181 aaaagaccac tgaacagacc gatactccaa gaacctgttt tccttttaaa acggtaagcc
     6241 tcaaaaagat tcaacaaccc aagcaaaaca agataggcgg tgatcagata accgataaaa
     6301 tgaaaaacag cagaagggtt tagcacaatc gcaatcccag aaataatata aacagcagct
     6361 cttaataagg cgtatttttg aatattttgt ttaatcgtct caaacatata agaaaacctc
     6421 ctttgactta ttttaacgtg aactagtcat tttaccaaat taaatgttac aaaatgagcg
     6481 aaggagaaga atatctctgg aaaaatcaac ttctgaggat cttttccatt tttttgcctt
     6541 ttgctagctc atcgatcatt ttatctaaat aacgaatttc tttcatcaag ttatcctcga
     6601 tgtcttctac tcgtacacca caaacaacac ctgtaatcaa ggaacgtgaa ggattgggga
     6661 caggggcttc cgcaaaaaat tgttcgaaag tcacttgttt ttgtagttga tattctagtt
     6721 ctgtttggga ataacccgtc agccaacaaa tgatttcatc tacttcctct tttatacgat
     6781 cttttctctc cgcttttgct acatacatag gataaacgct tgcaaaactt gtcgtaaaaa
     6841 tacgattttg cgccataatc agtcaccttt ctttttttca a
//
LOCUS       DACCXO010000131         6791 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.138, whole genome shotgun sequence.
ACCESSION   DACCXO010000131 DACCXO010000000
VERSION     DACCXO010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.138"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            25..915
                     /locus_tag="IVY70_002530"
     CDS             25..915
                     /locus_tag="IVY70_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP7959631.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(988..1812)
                     /gene="nadE"
                     /locus_tag="IVY70_002531"
     CDS             complement(988..1812)
                     /gene="nadE"
                     /locus_tag="IVY70_002531"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP7959632.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1824..3296)
                     /locus_tag="IVY70_002532"
     CDS             complement(1824..3296)
                     /locus_tag="IVY70_002532"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP7959633.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3933..4295)
                     /locus_tag="IVY70_002533"
     CDS             complement(3933..4295)
                     /locus_tag="IVY70_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP7959634.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4518..5270
                     /locus_tag="IVY70_002534"
     CDS             4518..5270
                     /locus_tag="IVY70_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7959635.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5410..6333)
                     /locus_tag="IVY70_002535"
     CDS             complement(5410..6333)
                     /locus_tag="IVY70_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7959636.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6348..6620)
                     /locus_tag="IVY70_002536"
     CDS             complement(6348..6620)
                     /locus_tag="IVY70_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959637.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2075 a         1352 c         1160 g         2204 t
ORIGIN      
        1 aaaacctttt aagaggtgat atatatgcaa ctacaaataa acgaagaatc cttaccggta
       61 tatgaagcat tagctagtaa aacaaggatc aagatcatcc agctactttc caaaaaaaag
      121 atgaatgtca aagatttggc aaaagaactt ggagtgagca gtgctatcac gacgatgcac
      181 gtgaaaaagc tagaggaagc aaacatcatc aaaacagaaa aagtaggaca acaaaaaatt
      241 tcgagccttc gtgtcgataa aatcgatatc agttttccag aaaaaatatt caatgctttt
      301 gatacgaaag aaacatcgat acctattggt cactatacaa attatgcaat tgaaccaaca
      361 tgcggtttag ctacgatcca tgattttatc gggaaagtgg atgaaccacg ctattttatg
      421 gatcctcgaa ggatggatgc ccgcattctc tggtttacca gcggatttgt cgagtatcag
      481 gcacctaatt ttttaaatac agaggataca ttggagatgt tggaagtttc tgtggaaatt
      541 tcttctgaat ttcctttttc aaatgacaat tggccttcag atatcacttt cagcctaaat
      601 ggtgtggagc taggaacatg gacgagccct ggagattttg ctgatatccg cggtaaatat
      661 acgcctgatt ggtatccaga taatcttaat caatacggct tgctgaagac gatccgaatt
      721 accaaacact tgacgaatat gaatggagaa cccttgtcaa acatcacgat caacgacatc
      781 cctaaagagc aagacacttg gcatttgcga atcgaagtga aagacgatgc caaacatgtg
      841 gggggctgta ctttatttgg taaaggattc ggtaattacg atcaggatat caaattaaaa
      901 gtttactaca gctgatattt ttcttttcta ttttataaaa caaaaacagc caatccctat
      961 gaagggacgg ctgtttttgc tttattgtta tttccagaaa tcatccaaaa cggaaattgg
     1021 caaatgtcgt ttatgttgtg tcttgttcca ccatgcttcg attttttctt gtgcttgagc
     1081 gggaacttct tttccttcta gataatcatc aatttcatta tatgtgacac ctagtgcgac
     1141 ttcatctgca atcattggtt tcccatcttc aagatctgca gtcggaattt ttgtgtataa
     1201 tttttccggt gcattcaatg cttgaagcaa ttgtttccct tgtcgtttgt ccaaacggaa
     1261 taatggcaaa atgtcggctc caccatcacc aaatttcgtg aagaatccag taatgttttc
     1321 tgcagcatga tctgtcccta atacggctcc tgctctttct ccagctacgg catattgcgt
     1381 aatcatacgt tgacgtgctt tgatatttcc tttgttaaaa tcgctgattc ccacacctgc
     1441 ttcgcttaat acttgtactt gtgcatcaac agctggtttg atatttactc gcagactaac
     1501 atcgggctgg ataaagtcaa gagcagcttt tgcatcttct tcatctgctt gttcaccata
     1561 aggaagacga acagcgataa attgatagtt ttcatctttt gtttcttcac gcatttcttc
     1621 catcgttagt tgcgccaaac ggcctgctaa agtagaatct tggccgccgc tgatacctag
     1681 tacaaaagtt ttaagaaacg gatatttttt caaatatgct ttcataaagt caatactttt
     1741 acgtatttct tcttttggat caattgttgg tttgacacct aattcattga taatttctgt
     1801 ctgcaggctc atttattctc ctcctatgct tctgtatgaa gttctgctac gctttttttc
     1861 attcgttcca tgatagccat tttatggttc cagcattcag tagacagatc gactgggtat
     1921 ttttgcggat tcaagtctcg tttatactct tcccatagtg aatctagatt ttcttttgta
     1981 tattctttga tttcgtttag tgcaggtaat tcgtaaactc tttttccttc gataaagata
     2041 tcttgtaaaa ctgggcgagc tgtaaagtct cgaacagttt tattgataaa cgtatgcact
     2101 ggatggaaca tgaagatagc ttcttcttct cgagggtctt catcccataa tgtcacgtag
     2161 tctccttcag atttcccatc aaaatttcga gtgatgcgcc acacttgttt tttccctgga
     2221 gtagttactt tttctgcatt gcttgataac ttgatcgtat ccatcatttt tccttcatca
     2281 tcttcgatcg atactagctt gaatactgca ccaagggctg gctgatcata ggctgtgatc
     2341 aattttgtcc caacacccca tacatcaatt ttagcttttt gcatctttaa attcaaaatc
     2401 gttgcttcat ccaagtcatt cgaagcatag atttttgctt ccgtaaatcc ggctgcatct
     2461 aattgttccc gaacacgctt agatatatag gccatatctc cactgtcgat gcgtacgcct
     2521 tggaagttga ttttgtcacc taattctttc gccacgcgaa tcgcacttgg cacacctgat
     2581 ttcaacgtat cataagtgtc gactaagaac acgcagtctt tatgggtttt agcgtaggcc
     2641 ataaatgctt cataatcatt tccataagat tggactaacg aatgagcatg ggtcccgcta
     2701 gctggtatcc caaatatctt cccagcacgt acattgcttg ttgcatctgc tccgccgata
     2761 tatgccgctc tagttcccca gactgctgca tccagttctt gtgcccgtct agttccaaat
     2821 tccaatagtg gatcttctcc gatgacagat ttgatacgag ctgcttttgt cgcgatcaac
     2881 gtttggaaat tgaccatatt caacagtgct gtttctacta attggcagtg cgctaaaggc
     2941 ccttccactt ggattaatgg ttcatttgca aaaactagtt ctccttcacg agcagaacga
     3001 actgttgctt taaattcaaa attttgtaaa tactccaaaa aaccttcagg atagacttct
     3061 aatccacgca agtaagcaat atcggaatca ttgaacgtca gattctctaa ataattgact
     3121 aaccgttcca atcctgcaaa aaccgcgtat ccgtggttaa aaggcatttc tcgaaagtaa
     3181 cattcaaaaa cagcatgtaa atcagctcga cctaattccc agtatgtctg catcatattg
     3241 atttgatata gatccgtatg cagcgtcaaa ctgtcgtctg ggtaagtatt attcatcttg
     3301 tattactcct cttggcaagt tttcatttcc tacccattat aacaaaaaaa attaatagga
     3361 aaagaaatct tccatttttt ctgaaaaagc acatgtttta cggatatttc ttgctaaaaa
     3421 tgaactccat ctgctaaaac tatcctttct tattggtatt taacctaaac gagaaagaat
     3481 acttatttta ttcaatgtat ccgagacacg aagaaattag cgttttctat ttttcttact
     3541 tgataaaata agtagatttt cctactacaa tcgtagccgt taagataccg atgattgaac
     3601 aaatataacc aaatgtctct acaacataat caatggcaaa agtttttgaa gcaataatac
     3661 gtctttcctt ttttgcttct tatccccaaa gaaacggata taacttatta tgtattgaat
     3721 ttttttggaa aggcgaatta tgacaagata taccttgcgt tgaaaaaact cgcggaaaaa
     3781 atatcacacg tttttaaaaa gatacaaagt aagaaagcag ctgtcaatag aatggaaaca
     3841 aaaatggcga gacatcaatc agtctcgcct tgttttttat tgtatacatt agatatggag
     3901 caggttacca taaagatgat tattagtcgt tattaaatag aatccttcgt atttttttgt
     3961 tctatcacta caatatcttt atttggacgt ttgaattgta ttccggcacg ttctagctcc
     4021 tgtttcactc ctacatggac tttgacatct tctcgtgtca tttttaaaac gtgatgaatc
     4081 gcatagtcga tttcttgcct tgtgattttt ttcttcgtat tgatcatcac gacttgcttt
     4141 ttatcatatg tgtctaccaa tagcagttgt gttcgatcta acgaaaataa tttgtaataa
     4201 ttaatagccg tattcccaaa aaagaattcc actactttag gataaaatgt cttaatattt
     4261 aaattactat tcacaggtgt ttcgattagc ttcatctcat tcctcttttc tatatagtcg
     4321 atttagatcc aatcttaagt tatgttacca tctttcctag cattaaggaa tcggttacaa
     4381 ttgaaccttt aaagacaaaa ggatttacct tttatcactt aatagtataa cataatttcg
     4441 tgagaaaaaa atacaatttt ttcttgacca tctcgttacg taaaggatta tgcttacatt
     4501 tgtcaggagg aaaaataatg gagtacacaa taaagaaaat gtcagagata tcaggagtca
     4561 gtccgagaac cttgcgcttt tatgatgaga ttggactgct aaaaccagct aggatcaatt
     4621 cttcaggtta tcggatttat ggcaaaaaag aagtcgatcg tctgcaacac atcttatttt
     4681 atcgaacaat ggcattcaag ttggatgata tccaagaggt cttagacaat ccatcttttg
     4741 accaccagaa agctttaatc aagcatcagc aaatgttgct ggaaaaacgg gcacaaatcg
     4801 ataccctctt gacaactgtc caacagacac ttgatatgta cgaaggagga agaaaaatga
     4861 gtgacaaaga aaaatttgaa gggtttaaac aacaaaagtt gaaagaaaat gaagaatcct
     4921 atggacaaga gattcgacaa aaatacggaa aaaaaaaggt tacagcatcc aatgaaaaat
     4981 ggatgaatat ggataaagaa acgtatgaag aaatgcaaac tgttgaaaag cagatgttat
     5041 ttgatctcac tctttactta aaagaaccag aagatgaaag cttagcagac cgtatcttcc
     5101 aagctcataa gaagtggttc tcttacagct ggcctgaata tcagccaaaa gcgcataaag
     5161 gactaggaat gatgtacgaa gccgatgaac ggttcactgc atattatgac gagcgaagtg
     5221 gtcatggtgc agcaaaagct ttaaatgaaa ttatccaaaa aaaggcgtaa tcttttacat
     5281 gatccaacaa aaaagtacac gtaaaatgac ctaataacaa aacagcagac acccaagtta
     5341 gatttttatg ctaacttttg gtgtctgctc cattctttca caagtttttt catcctactt
     5401 taaatatatt catcgttgaa taaacgtata aggtattttc actttatcct ttttttctgt
     5461 aagaaacaat tgaaatgcca tttctccgca agcttccaga tggtggtcga tggttgggaa
     5521 accaagaagc tcgctgatca gcaaattatc gcgtccaatc accactggtg tcttatcttg
     5581 cagatagctt tgcaagatgc ctgctgcaac catatccgaa tttgtaaaga caccatcaac
     5641 tccaagctct ttaaaaaaga agcctgcttg ttttcctttc tctgcatcta tacaatccca
     5701 aaaaacaagt gattcatcaa aagatggaaa aagttgatga catagctgca aagtgatttt
     5761 tgagttataa cttagcctgc cacttcgccc caacgtgatg cccaattgct ttataccttg
     5821 ctgcttcaaa taagtaagac cttctgtaat tgattgttct cgatcaataa agacacagct
     5881 tgcctgtttt cccttgattt cttcacaaaa aactagaggt ccatattttt gatatgccaa
     5941 taaatcatct agtgtattgg cacgagacgt aatgatcaat ccatcgaaag ctttagctgc
     6001 aaattcttca agataccttc gctctttttc tttatgatag cccgtcggta aaagtgtcac
     6061 tttgtaatca taatcaaacg ctgcttcaat gatcccactg atcaactgat caaaatacgg
     6121 atgattggta taaggcagaa tcactccaat gcttttcgtt tttccataac ttaactgtct
     6181 ggcatttgca ttagggatat aatcgacttc tttcatgacc gcaagtattt ctttcctttt
     6241 ttcctcatct acatatggat gtccattgat tacacgtgaa atagtagaaa ctgaataacc
     6301 tgtgagcttt gcgatatctc gaatgtttgc cactgctgct tcgccaccta tacaaaaaga
     6361 ttttcttgta aatagtctac cacagctgga ccgcattcaa aaaactgata catttttcct
     6421 tcatgcgaaa aactgatccg ctgcccgccc caatatatat cttttgtcaa attttcgatt
     6481 tcattcattg gtatttcttt tactatattc ctattcaacc cttgaaaact gtacaacgtt
     6541 aatacggatc ccttgaactc gacaaacgca taaaaataat tgttcgttgt gccatctgtt
     6601 atctctacat aagtattcat tgcaagccct ccttttgata ctttgagtat agtgtatgaa
     6661 acgcgtctca tagcaaggag tttgttttgc caaagaaaag ttttattcct ctttcattat
     6721 taccaaagtc tcttatttta aattgaatac attataatga tactgtaaat atatctttat
     6781 tattagtgaa t
//
LOCUS       DACCXO010000132         6702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.139, whole genome shotgun sequence.
ACCESSION   DACCXO010000132 DACCXO010000000
VERSION     DACCXO010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.139"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            141..962
                     /locus_tag="IVY70_002537"
     CDS             141..962
                     /locus_tag="IVY70_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP7959638.1"
                     /translation="MGLLVPRANTYTIPLTKRQLIAKRYQRITRAINREFWNSESDTA
                     HSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQVIKNAFQEILPN
                     SDIRADGQVVKINFHDGIKFEIVPAFNEKDYLGESKGFIYPDSNMGGNWKATNPKKEQ
                     EAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEAMGNWKFVENNSG
                     GQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICLEKVLKKIAL"
     gene            982..1542
                     /locus_tag="IVY70_002538"
     CDS             982..1542
                     /locus_tag="IVY70_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HAP7959639.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNKTHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            2450..3217
                     /locus_tag="IVY70_002539"
     CDS             2450..3217
                     /locus_tag="IVY70_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP7959640.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            3243..3788
                     /locus_tag="IVY70_002540"
     CDS             3243..3788
                     /locus_tag="IVY70_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959641.1"
                     /translation="MGERTQALITVKYGNSKTKVSVSIHYQWGYGRFMLMDVLNQGIY
                     LINKAYDLRDESREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            3789..4355
                     /locus_tag="IVY70_002541"
     CDS             3789..4355
                     /locus_tag="IVY70_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959642.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTIEGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            4370..4618
                     /locus_tag="IVY70_002542"
     CDS             4370..4618
                     /locus_tag="IVY70_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959643.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            4637..4876
                     /locus_tag="IVY70_002543"
     CDS             4637..4876
                     /locus_tag="IVY70_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959644.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            4893..5477
                     /locus_tag="IVY70_002544"
     CDS             4893..5477
                     /locus_tag="IVY70_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959645.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            5584..5865
                     /locus_tag="IVY70_002545"
     CDS             5584..5865
                     /locus_tag="IVY70_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP7959646.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDMPVDVLDTDEKLEEWFNEA"
     gene            5855..6196
                     /locus_tag="IVY70_002546"
     CDS             5855..6196
                     /locus_tag="IVY70_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7959647.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
BASE COUNT         2525 a          871 c         1371 g         1935 t
ORIGIN      
        1 actttcaatt attgtgaaat atttcccttt aattaatcaa attttttata aaagtaatat
       61 ataaaataaa ttgcttgaag tattatattt agatatcata atttatggaa gaaaaattaa
      121 ttaaaaagga tgagattata atggggttac tggttccgag agctaatact tatactatac
      181 ctttaacgaa acgccaatta attgctaaaa gatatcaaag gattactaga gccatcaata
      241 gagagttttg gaattctgaa agtgatactg ctcacagttt atacgtaggt tcttatggga
      301 gaggaacggc aattagtact agtgatatag atataatagt tgaattaccg atggcagaat
      361 ttgacagatt caaaaattat ttatcaaatg gaccgtctaa gttattacaa gttataaaaa
      421 acgcatttca ggaaatacta ccaaatagtg atattagagc tgatggacaa gttgttaaaa
      481 taaatttcca tgatggaatc aaatttgaaa tagttccagc ttttaatgaa aaagattatt
      541 tgggtgagtc aaaagggttt atttatccag attcaaacat gggagggaac tggaaagcta
      601 caaatcccaa aaaagagcaa gaagccatga aattaaagaa tacaaaaagt aataatttat
      661 tatatgctac gtgtaaacat tttcgacatg tacgagatac agaatttacg agttatcatc
      721 tttcaggaat agtaattgac agtttcgtat atgaggctat gggaaattgg aaattcgttg
      781 aaaataacag tgggggtcaa aacattagtt ctgtatctta tgagacagct ttattagaat
      841 actataactc acataaagtt atgggcggtt taaatttata ctctcctgga agcaatcaat
      901 ttgttaattc tgatagtagt atcatatgtt tagaaaaagt attaaaaaaa atagctttat
      961 aaagttttat ggaggaacaa catgggagac gaaaaaaagc gcttggaagt tcaattacgt
     1021 gaagcatatg gacgagtaac gtatacttac accactcatt taaagcaagc cgataatatt
     1081 atttctgaca ataaaatttt aaggtgtctt caaattggtt tgtcagccct tactactgga
     1141 ggggtactta gtaccattat tactgataat agtgagttaa cctttgttgc tggattatgt
     1201 tcaacaattt ctcttggatt aaacttatac tttaagaatt ttgatcttgc tgaagaagct
     1261 aaaaatcatc aaattgttgc cgataaatta tggttgatca gagaagaata cgtaagctta
     1321 ctaacagatt taaatcgttt aagtgttgaa gatattgtga aaaaaagaga tcaattaatg
     1381 aacaaaactc atgaagcata taaaaatgcc cctaaaacaa atagtaaaag ctatgaatca
     1441 gctcagaaag cattaaaatc tgaagaagag caatttttta cagaagagga attggataag
     1501 atgttaccag agcatcttag aaaaaagagt tctaaatatt gaactctttt tttatgctta
     1561 aaattttctt gtaatttttc tattcaatca ataaataatc tatatttaag aagaaatatg
     1621 atgatagaaa catctcctaa agccaccgca agccgtccta gccattgccc agtccgacat
     1681 cgctagatta gtgcatctct ccaaaaaagt tttctaaagc caccgcaagc cgtcctagcc
     1741 attgcctagt ccgacatcgc tagattagtg catctctcca taaaagtttt ctaaagccac
     1801 cgcaagccgt tctaagcata gcttagcccg acattgctag atatgctcat ctcaccatga
     1861 acgtttccta aagccaccgc aaaccgtcct agccatcgcc tagtgcctag tccgattttg
     1921 cttgatcggt tccatttctg tttatttagt gggtcaacac cgtaatctta aatagccatc
     1981 gcctatttaa gattactagg tctgctttga aggtaaaaag tctttcattc ttcaagactt
     2041 ttaggctttc aagctacgcc tgaaagcatt gaaattcatc gctctttatt tggttttgcc
     2101 atttataatt ccttacgccc attatctttt tcgcctgtca taccttgtca gaagtgttta
     2161 gagcttatct catctttgga ttaatacaag tggcgtaaat ttttttctaa acgcaagcgt
     2221 tacgaaaata atttacttaa acacttgtat taatccggca gataagaacg cattctaatc
     2281 actcccgaca aggcaagaca ggcgaaaaag ataaataaat gaacgaaagg aagaaacata
     2341 aaatgcaaaa ccaaatagcg atgaagaaaa aaccacctcc ctctaaaaca atccaaacaa
     2401 aaaagaattt tgaaaaacca aaaatcaaaa acagaaagaa gcgttcaaca tgagagatag
     2461 aattttagaa gttaatcaag aaatggtaaa caagttaaca aatatcagtg ggaactatga
     2521 aaagtacgat ggtactttac ttgataatta tatttttcac gatacacaaa atatcagaat
     2581 tggcagaagt aaaccgagag cgttcattct catcaaagag aaatatttga atgaatggaa
     2641 tagcacctat gaaatggtta tgactgataa ctttgaaaag gtattggatt tcagaaatgc
     2701 tttgttgggt tctgatatgg gaaatagcag tcttgaatct attgaagttg aaaacaagac
     2761 cgagaatgaa ggtgaaggat tgattaagaa tgtcctgaaa atcgaacaaa aatatatcag
     2821 tgaagataga agtttttata gagtcacaag ctcggttggt ctagcaaact ttgctactaa
     2881 cttaattgga aacaatgcta gtgaatcttt gctagtgatg tgtctgaata caaagaatga
     2941 ggtcattgct tatagcgaag ttttcaaagg tggaattaat caatcagtag cgataccaag
     3001 agagattttt caactagctt tgctaaacaa ttcagcaaaa atcgcagtat cacataatca
     3061 tccgagcgga tatgaatttc caagtgaaaa tgacaaaagt tttactacga atttgaaaaa
     3121 cgcaggaaaa acaattggta ttgaattgtt agaccatatc atcgtaacaa gtaaaaacac
     3181 aagctattac tcatttcaag aaaatggttc tttataaaaa ttagagaaaa gaggaaaaca
     3241 agatgggcga aagaacacaa gcgttaatta cagtcaaata tggtaacagc aaaacgaaag
     3301 taagcgtgtc aattcattat cagtggggat atggtcgatt catgttgatg gacgttttga
     3361 atcaaggaat atatttaatc aataaagcat atgacttacg agatgaatca agagaagaat
     3421 tagcaaaagg aatctttgat agaagtacag gagttatcaa ttttgtcacc tatgaatctg
     3481 gagtgagtgc ttatgatgaa acagggtttg gtgggcgcta tgaaccttat caagaaacga
     3541 ttgaagatgt gatgaagttt tcagataaca atgatggcta tgctcattta gagatggtgt
     3601 tcgatgaaaa tggatttagt ctagatgata aaaccagatt gcgattgttt gattttgagg
     3661 gtcaacagat aggactaaac aagttttgtg aaagcggaaa agagtttgct acgaaagatt
     3721 ttattaaggg atacagatta ttgctagaaa attatggaat aaacctagaa gaagagagcg
     3781 tgaaataagt gtttgaaaaa agaaaagacc agttccctaa tttagaagcg aaaagtcaaa
     3841 aagtgattga tggtgaaatg attgaattat tccaaaaaag ttatggaacc atagaaacta
     3901 ccgcaagttg caaatactac tttcaaggaa agagaaacac gtttaaaggc tttgccgatt
     3961 ctgatgaaga attggcaaag aaaatcattg attggcttgt cagcagtgaa cacgtagcaa
     4021 gagaagatac cgttagaaca acgattgagg gtgaagtcct aacaggtact agatgtgttt
     4081 gttgtaatca tgaatttgta gaagatgaag gttgctaccg aatcaatgaa tatggtgatt
     4141 ttatttgcca gagttgccac gagatagaat ttccaaccga aagaagttgg tttgaataca
     4201 tgctacaaga ttatgagatg gaaacagaaa atggtgaagt agttttttgt accaaagagg
     4261 aattgagtaa gagaagcgat gatgaaattg aagattggtg tgtgtggtta gtagggacag
     4321 atgattgcgt tgcatattac acaacagcag aataggagtg tgaatcagca tggaaggaat
     4381 ggattttata gatcatgaag atttgattga ttttggttat acatggaaag gaatggtagg
     4441 tattagcaga agtcttgcta atgcctttta cgaaagaaat tatgcagtct atgtccttta
     4501 tgatgacaat acagaaagcc ttgttgatga agagtataaa ttggatttag agaatgtttt
     4561 atatgggata gaaaaagaag atttagcaaa atatattttt tcatggttgg gtcaataaac
     4621 gaaagagggt agcaacatga caaaattcaa aagtaaaggt atgcaatggt gttcttatta
     4681 ttatgttggg aatgatgaag agcttttgag aaaagccaaa caaaaaggtt atccagataa
     4741 gatgttaaaa cagattggct attacatgcc agaagagaga atagaaaaag aatcttgtaa
     4801 ggttcttttt gatagtgaac agctattagt tttgttaact caaaataaat tgatttgtca
     4861 atttgaaaat aattaatatg gggtgaagaa ccatgaatga gaataagatg gtattaagtg
     4921 aatacgcaag attgagaacc attaattggt tgaatggtat gcaaagtagt ggcgcttttg
     4981 atggatttga tgaggagaat atggactttg aaagtttatc gaatgaggat attagatatt
     5041 attttatcga atggtatttt acagaaccag atgaagaggt ggaagatgaa ccagaattaa
     5101 ttttagaacg tcaaagaatt ttattcagtg aacaagatag taagaggttg tatggtttat
     5161 atgacttaca actgaaagga ttaaaagcgc agtatgttgc tgatagttac gatgatatgg
     5221 tagaacaggg ggcagatagg gttgtcactt attcagaaga tgatgaagag gaatgggaaa
     5281 actattcaga tgatgagata tgctttaatg atgggtatat gcctattgaa atcaccgaag
     5341 aagaatatga taaagtgtcg gatagcgatg agattggtat gtgtacggtt gtaagtggat
     5401 tggatataga tgtccaaaga gtagaggttg cataccagaa gatgaaagaa cgtataaaaa
     5461 gtaaagtaga atcttgattt gattggattg tttgcaaagt agctaattaa gagtataata
     5521 taatcataaa tgtagttaat ggatataata atgagtatat tatactcaaa agaggtgaac
     5581 gaaatgaagg taaaaaatcc aaaagaaaaa agcagaattc aagtcggaat agacaaggat
     5641 ttaaaagaaa acgcagaaat gatattggaa gagctagggt taaatccaac aacagcaatc
     5701 acaatccttt ataagcaagt ggtcgctaga ggtgagtttc cagtagagat aaagctaagc
     5761 gaagaggaaa agcaaggaat cagattgcaa cagctaacta tggatatgcc agttgatgtt
     5821 ctagatacag atgaaaagtt agaagagtgg ttcaatgaag cctaatgaga tagtaacggt
     5881 ctatgttgca tttacagata aaaatggtgg taaaagaaga cctattttgg tggttagtga
     5941 taaagaagat agagtagaat tttttggaat cacaagtcaa tatgagaata aatcagacca
     6001 aataaagaga gtgtattttc ctattgcaga atgggaaaaa gtaggtctaa agaagcaatc
     6061 atggattgac gtagggtctt taaaggctat tcccaaaagc cgagagaaca tatcatttaa
     6121 aaaggttggt atgctatcag ctattgatac gaaaaagcta aataggttta tcgagagatt
     6181 acaaagaaaa gtctaacaag gcttttcttt ttttgtttat atgtatatta agatatacgt
     6241 ttatataaac gtatttatgt atattaaaat acacgtaaat atatacgtga ttataaaaat
     6301 taagaaaata ttaagtattg ttaaatagtg ataattagta atatacgaat cataagatac
     6361 attttatatt taatgtatgt aaataaataa aatgactgac ggatattgaa tagtaataaa
     6421 taacaataat tcagacggat acagtgagtg aagtagcttg ctacgaacga acaggcagaa
     6481 tgaaacggta gtttcattag agcaagtcgc ttgcgacgta gcgttaccac tgatgtaact
     6541 ggcaagttac atagagagag tttgcttgca aacgaacgtt acccacttga atagaaaaca
     6601 aaaataacat attagtgaat tttgaatagg agccacaact caaccaaccg cttgcggtgg
     6661 gtgagtccca cggaggaaaa gacgaacgaa cggtcgcgta gc
//
LOCUS       DACCXO010000133         6632 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.140, whole genome shotgun sequence.
ACCESSION   DACCXO010000133 DACCXO010000000
VERSION     DACCXO010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.140"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            148..2823
                     /locus_tag="IVY70_002547"
     CDS             148..2823
                     /locus_tag="IVY70_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HAP7959648.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3005..3244
                     /locus_tag="IVY70_002548"
     CDS             3005..3244
                     /locus_tag="IVY70_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959649.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3260..4633
                     /locus_tag="IVY70_002549"
     CDS             3260..4633
                     /locus_tag="IVY70_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7959650.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4637..6271
                     /locus_tag="IVY70_002550"
     CDS             4637..6271
                     /locus_tag="IVY70_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7959651.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6434..>6632
                     /locus_tag="IVY70_002551"
     CDS             6434..>6632
                     /locus_tag="IVY70_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959652.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2322 a         1071 c         1332 g         1907 t
ORIGIN      
        1 ttgtgatttc caaaatcaca acttttttct tttgtcaaga ttttgagtgg gatatatttt
       61 tgtagaactg aagatcatga aattatgaga ggttggaggt aatgtagtca tacaaataaa
      121 aattttttag aaagtgagtg gtaattaatg aaaatgaatc acacttcaat tcagaagcat
      181 ttacaactgt taaatacact ggggatttct tatgttaatt tgagaacttt ttctacatta
      241 aacaagaaaa ccgtttctca tattttttct actgcagatt ctcaagcaat tattaactat
      301 ttagaaaaac agaaattgtc tgaaggtaca gtaaacataa cttataatcc tattaaacaa
      361 gaggtgatta cacaaaaaaa gcattctgtg agagacaatg atattgaaaa aattcggttt
      421 gtatttattg atattgaccc taaaaggaaa gagggaagtg caacagatag cgaaaagaaa
      481 aaggcagaaa atgttatgga acaagtagaa cgttatttga aagaaaagag aatagaatca
      541 tttgtaaaag ttgatagtgg gaatggctac catatttttc ttcccataaa tgaacaacca
      601 aataatcacg agacgattct tactattcag aattttcttc aattacttca ccgaagattt
      661 ggagtagaag atgaagttga tattgatact tctgtttata atccttcaag attatgcaaa
      721 ttagttggta caccagcggt caaaggaaag tccacaaaag agcgtccgca tcgtttatct
      781 agtttccttt ctattcccga taacatagat agaagtaatt gttttaaaat tcttgaacag
      841 atagttgaag aaaattccat gatgtcaacc tctagagtaa aaagccatcc atcttttaga
      901 gaagtgttac cttttattca agcagactat aaacaatggt tagctcacta tcctgaatta
      961 acttatacca taaaagaggg cgatcataaa ggagttacct tacttatatt tgacgaatgt
     1021 ccattaagaa aacacacgaa taattctaat ggttcttgtt tgtctgttac ggctgataat
     1081 aagattcgat ttcattgtct gcatgcttcg gataaaaata aagatattca agactttgta
     1141 aaaaaatatc cgttgccaaa acctgtaaat ccttctgtag aatcattaga atccaatcaa
     1201 gaatattatt ttgaccagtt taaactgaat caagaaggag tatttttggt gaaagaaggt
     1261 caagaagaaa aagttgcttg tccgatgttt attaagcaaa tacgcaaaaa tatcgaacaa
     1321 aaagtcatta cacttgttct tgcctattat gtcgatcatt gttgggatga aatagaaata
     1381 gaaggtggat gtttacaaac taataatttt aaacaattag caaaacatgg cttaatcatt
     1441 aaagctagaa gagaagctga agtcgttgac tttttacaga ttcaacggga gaatgctcca
     1501 atcttttttg aacatgaatc tcttggctgg aaaaaagaaa atgaagcaat gatttttcag
     1561 ttacaagatt cttttggacg tgaaattcct tctgtattaa ctaaaaactc atgctaccaa
     1621 ttaacaacta atggtacaga agcggaattt gacgcattgg ttcataagta tgttttagga
     1681 acgaacttag aacttgcttg gtcgattggt attgcttcta ttgtgttagg ctatttaaac
     1741 gttacgaagg ctaatacttt tttgagttta ctaattaacc tacagggcaa gagcacaacg
     1801 ggtaaaacca ctacgttaca ctttattgcc tctttatttg gaaatccaac tacggttctt
     1861 agaaatatga atgctaccaa taaagccatt ataaagctag catcaaacaa tcagggaggt
     1921 gttccactaa tactagatga gttaggagca gcaactcaaa ataactttag ctcgttgatc
     1981 tatcaattag cttcaggaga agaaaggcta agattagatt ctaattgtca gcttatgcca
     2041 caagagactt tttctgtttt gacccttacc tcttctgaag agcgtttgga aaattatttc
     2101 aatgagaaat taactggttt gaaagttcga tatatcgaat tttctgatat caagtttaca
     2161 aaggatgcga atcacgcaga gaaagttaaa agtctttctt ctaatacttt tggttggggt
     2221 ataaagttat ttcttttgaa actctttgaa caagggatta caaaaattga agcaactttt
     2281 gtcgaagctc aaaaaaaact atgggctgtt ttacctgaac attctttgaa gcaacgcatt
     2341 ggtaataatc tagctttgat ttatagtgga gccctcctaa ttcaagatat actgggctat
     2401 cctttacgat tagagcatat acagacggtg cttattaatt cgtacgaaca gttaattgta
     2461 gatactagtt taggaagtga gcaaacggta tttgaccgag tgaaaacatt gttggtgatg
     2521 aatagccata aatttttaac aaacagtgac aacaaacata cgcccagtac gatttgggga
     2581 aaagtatcaa ttcaaaaaaa tggggatatt aaggtaaata tttttcctat cccgtttgag
     2641 gacttgttaa ggcgagaatt tcaagtaaag gatctaacct atctttttag gcaattgctt
     2701 gattcgggta atatgtgtgc agaaaaaggg agaagaacga aacgtattca catgaatcgt
     2761 caagttcttc caacatacga gattctatta ccttcttcac tcaaatcgta ttttcgattg
     2821 taatttagaa taaaccaaag tatgaaagtg aggcaccatg ttatggaaat atttcttatt
     2881 gatttagcaa aacaactgat tacttatctg tatgatagcg agcaaaccca aacggaagta
     2941 aatgaagctt gatctatgct tcatttactt cattcaatca aacatgaaag aaaggaaaat
     3001 tctaatgact aaaaataaaa tgacactaaa agcagaggtt cttctgtata ttcaagaaca
     3061 tttttcaaat caagcctttt ttacaaaacc aatttacttg gatttcgaaa taagaggggt
     3121 atctgctgga tctatcgggg gtactcttca agcgttgaag aacgaaggat atttagaaaa
     3181 tcactttgtg caacgttcgt tcaatagaag agtcgtgaaa aaatggtatt tagttcattc
     3241 atagagaggg gaatcaagca tgcgtgtggc ttgttatgat cgaacgtctg tttatgaaga
     3301 agcgaaaatt agtaatgacg ctcaatatga tgcagtgaaa gagtatgttt tagagaaata
     3361 tgaacaaatt ccccaacgat tttcagacaa aggagaatca ggggaaaaag cggagcgtcc
     3421 agcttttcga cagcttagag cttgggtttg tgcgtcagaa gaagaggggg tgatctatgt
     3481 ttggcgttat gatcgattgg cacgtcaagc caaaatagcc ttagaattta ttgagttgtg
     3541 ccaacaacat caggttcaag tgtattcgat ttctgaacct cttcctgctt ctttctcaca
     3601 cgcgtcaacg aataaactat ttgtgcaatt attgttcact tttgcagaac aacaacgaaa
     3661 cgtcattatt gagaatgttc gtgccggact ttcctataaa aagcaacaac ggcaatattt
     3721 gtcttctgcg gtaccatatg ggtatcgttt agtgaatggg aaaatccaac aagaagtaca
     3781 cgaagcaagg attgttcgct atatttttca actttatttg accaaaaaat atggctacaa
     3841 aaagctttgt caacgattga cgcaacaaaa gcttttcttc cgcgaacgtc cttttcagcc
     3901 ctatcatatt tatagtatcc taaaaaatcc attgtactat ggcgaaatca aaggaggatc
     3961 tttggggaag tatctaggaa cctttgaacc gattctctcg aaagcaacct ttttacaagc
     4021 ccaagagatt cgccaatcaa ggtgtacagc taaaaaagat acgtatctgt acttattacg
     4081 tcaaaaaatt cggtgtccct tctgtggtcg tcatctgagt tcgaagtatc aatggaatac
     4141 aaagaagacg aagacgcttc attattatca ttgtaccgac cgaagctgcc aaggaatttt
     4201 tcttcgggca gaaagggttg agtcagaagt attggaaaag atacaggctt tttttcaata
     4261 cgaaggcgtc tatcaagcct tgattgccca attaacgcaa caattagccg aaaagcgaaa
     4321 agatgaaaaa cgcaaagtaa tccaacaaaa gaaacaagaa gcacaatgga ttaaggcatt
     4381 tgaagagggc aattgttcac tggaagaact caagcaacaa ctgcaagctc ttcaaaagcc
     4441 gatgtctact tcttcgtttc tttcactcca acagtgtgaa gcaaaagtga aagaatggct
     4501 tcaactaaaa gacctctcta gtcgacaatt attatgtgac tatgtttgtc ggattgaggt
     4561 aacaaaggat aaaacagttt gtgggattta cttccgacaa ctgacacaaa atattttaga
     4621 aaggaacgaa tgatctatgg aaatgataca aaccaaacga gtcgcattgt atattcgcgt
     4681 gagtacacaa gaacaagctt tagaaggaca tagtattcaa gcacaagaac aaaatggacg
     4741 acagtttgcc gaacggatgg gctatgaagt ggtagaagtt tatgccgatg aaggaatcag
     4801 tgggaaatcc acaaaaaatc ggttcgccta tcaacgaatg atgaacgatg ctcggaacca
     4861 tcgttttgat ctactagtga tttggaaatt gacccgtctc ggacgcaata tgttagatgt
     4921 cttacaaaca gtcgaggaat tattgcgata tgacgtagga ttacattcga tctcggaaca
     4981 atttgacgtg accacttcga caggtaaatt gatgttacaa ctccttggga gtttcggtga
     5041 atttgaacgc aaccaaattg cggagaatgt tcaaatgtca atgcaaagct tagtgaaaga
     5101 taaaaagcgt tatgcaggag gccgacgttt aggctatgtc agtggtattg acacgaatgg
     5161 tcaaaaacaa ttggtcattg aaccagaaga ggcgaaaatt gttcgtctga tctatgcaaa
     5221 atatttaggc ggagcagggt atcgagcgat tgccaatttt ttgaatcgtc aaggatatca
     5281 aacagtcaaa aagaatgctt tttctaccac ggcagtcaaa gatatcttac gcaataaaat
     5341 ttatgcaggg tatttagaat atgcgcgcta catcaattgg gaaaccaaac gacggaaggg
     5401 gaagaatccg agaccgattt tagtgaaagg aacccatgaa ccgattattt cggaagaaga
     5461 ttatcaaaaa gtacaagaac gccttgcttt agaaggaaaa caacccaaat ggaaccatac
     5521 aggtgaaaat gtattgacag gcttgttacg ttgtccagaa tgtggcgcac cgatggcagc
     5581 gagtaatgtc acaaataccc taaaggatgg cacaaagaaa cggattcgat attattcttg
     5641 ttcagtcttt cggaataaag gagcaagtgt gtgtcatgcc aattcgattc gtgccgaaca
     5701 agcggaaaca tttgtcgccg accgattaaa ggagatcgtt cagctaccag aagttcttcc
     5761 ccgattggtg gcagcgttaa atgaagaaat agtgcgacaa agccaaccat tggaacaaga
     5821 actcgtggtg ttactggaac gaaaggaaga attgaagact aaaattgaaa aatgggaagc
     5881 tgctttggaa gatagtcctg aattgtttcc gatgttaaaa gaccgcttag acgaattgac
     5941 tgaaaaacga cgacagttgc acattcgaga aaatgaaatc ttagggatct ttcaacaagg
     6001 agagccgatt caagtcaaag atgtccaacg gatattgact agtttggatc gttttcttgc
     6061 ccagagtgag aagaagcaaa tcaaagcctt gtatcgtacc tttatagaga aaataacctt
     6121 tgatcctaac cataaggaga tgttggaaat tacgatgaaa ttcacgcatg ccgtggtaca
     6181 acaattgaat gagcaatatc aaatagccgt atccaaaaca aaggatacgg ctatttttgt
     6241 gttaaaaaca ccctttacgt taacgatcta gtctaggtta tatcaaacaa cgttagaggg
     6301 gcgctaacgc ttctctagcg atttgaaatc gttagagaag tagttaggga ggtccttatc
     6361 ataaataggg atatttatta atgaatattt gaaatataat tgtatataag aattaaaaag
     6421 agtggggata gatatgaaaa agatgggtaa gtgtatttta tgtgaaaaat acactgaact
     6481 tactaaagaa catgtaccac ctaaaaaatc agggaatact ggaggtaaaa agaccagaac
     6541 tggtaattta gatgatttcc ttaagagcga ttttacaaaa ggtgattttc cgaaggggat
     6601 taaaaggaaa cctcaaggaa atgtttatta ta
//
LOCUS       DACCXO010000134         6594 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.141, whole genome shotgun sequence.
ACCESSION   DACCXO010000134 DACCXO010000000
VERSION     DACCXO010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.141"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..1479
                     /locus_tag="IVY70_002552"
     CDS             <1..1479
                     /locus_tag="IVY70_002552"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP7959653.1"
                     /translation="SDRLLEQVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIA
                     TIVDILGYAISPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQS
                     KKLGTDEQQRNVTITEVIKKLNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYT
                     PHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTT
                     TYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTF
                     LDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKL
                     LEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSE
                     ENIQKILETYAERKDVEKYAHLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGND
                     IKKIRQEQQVLEKELLEAISSLQTTPENEAWLQGALEVFKHE"
     gene            1472..2716
                     /locus_tag="IVY70_002553"
     CDS             1472..2716
                     /locus_tag="IVY70_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP7959654.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELIKFKLEPGDFIMSGAGTIGRIS
                     RVPKQIKPGVFNQALIRLRINKEITDSEYFIQFIRADFMQRKLTGANPGSAITNLVPM
                     SEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLKETKKGFLQKMFPKNGAK
                     VPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGSRL
                     DDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEFIL
                     QFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKEEQQKIGAFFKQLDDTIA
                     LHQRKLDLLKETKKGFLQKMFV"
     gene            2730..5879
                     /locus_tag="IVY70_002554"
     CDS             2730..5879
                     /locus_tag="IVY70_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP7959655.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         2338 a         1081 c         1361 g         1814 t
ORIGIN      
        1 tccgaccgtt tgttggaaca agtggttctt ttagcagatg aatccttaga ggaatatgat
       61 acggtaagca aacaaacgat gttgtatcgc gaattacttt cagatgaaga atcaaaagaa
      121 gatctgattg caacaatcgt tgatattttg gggtatgcga tttcgccaga gtatcttttc
      181 aacgtcttag cggatcaagc aaaacaagca acgttccaat tgaatgatct aaacaaagcc
      241 tttgtgcaat tggcatctac ctacaatcaa tttaatgggt tatttgatga tgtggatctc
      301 caatcaaaaa aactaggaac agatgagcaa caacgaaatg tgacgattac cgaagtcatt
      361 aagaaattga atgacgtgga agttttaggt cacgatgggg acgtgatcgg ggatgcttat
      421 gaatttctga tcagtcaatt tgcttcagaa gcagggaaga aagccggaga attttacacg
      481 cctcatatgg tttctgatat gatggcacaa atcgtgacgt tggatcaaaa agaacgacga
      541 ttctttagtg tttttgatcc aaccatgggt tcaggttcat tgatgttaaa tgtgcgtaac
      601 tacttaactc acccagataa cgttaaatat catgggcaag aattaaatac aacgacatac
      661 aatctagcaa aaatgaactt gattttacat ggggtagatg ccgaagaaat gaatctccgc
      721 aatggggata cgttgaataa ggattggcca acagatgaac cttacacatt tgatgcagta
      781 gtaatgaacc caccttattc ggctaattgg tcagcagata cgaccttctt agacgattct
      841 cggtttaatc gttatggaaa attagcgcca aaatccaaag cagattttgc gtttcttctt
      901 catggtttct atcatttgaa ggaaacaggg actatggcca tcgtcttgcc acacggagtt
      961 ttattccgtg gcgctgcaga aggggtcatt cgtcaaaaat tactagaaga cggcagtatt
     1021 tatgctgtga tcggtatgcc tgcgaacttg ttcttcggga catcgattcc aacgacggtg
     1081 attgtattga aaaagaaccg ccaaactcga gacgtattat tcattgacgc tagtcgagaa
     1141 tttgtcaaag gaaaaaatca gaataaatta tcggaagaaa acattcaaaa aatcctcgaa
     1201 acctatgctg aaagaaaaga tgtcgagaaa tacgctcatt tggcaaactt tgatgaaatc
     1261 aaagaaaatg actataactt aaatattcca cgctatgtag atacgtttga agaagaagaa
     1321 ccaattgata tggttcatgt cggaaatgac atcaagaaga tacgacaaga acagcaagtg
     1381 ctagaaaaag aacttttaga agctatctct tccctgcaaa caacacctga aaatgaagcg
     1441 tggttacaag gtgcgctgga ggtgttcaaa catgagtaat gatatacaac cagaaattcg
     1501 tttcccagga tttacggaag cttgggaaca gcgtaagttt gaatgcttac ttgataaaaa
     1561 agatggagta aggaggggac catttgggag tgccttaaag aaagaattct ttgtatctaa
     1621 tagcaatttt gttgtctatg aacaacaaaa tgctatttat gataattacg aaacaagata
     1681 taaaattact gaaaaaaaat ataatgaatt aatcaaattt aaattagaac caggcgattt
     1741 tatcatgagt ggcgctggaa caattgggag aatttcaaga gtaccaaagc aaataaaacc
     1801 gggtgtattc aatcaggctt tgattagact tagaataaat aaagaaatca cagattcaga
     1861 atattttatt caatttattc gtgcagattt tatgcaaaga aaactcacag gagctaatcc
     1921 aggttcagca attacaaatc tcgtaccaat gtcagaagtg aaaaaatgga tagtacaatt
     1981 tccaatatta gaagaacaaa agaaaattgg gaacttcttc aaacaactgg acgacactat
     2041 cgctcttcaa caacgtaagc tagatttatt aaaggaaacg aaaaaaggat tcttacaaaa
     2101 aatgttccct aaaaacggag caaaagtgcc agaaattcgg tttcctggat ttacggaaga
     2161 ttgggaacag cgtaagttca aagagtttag taagaagact ggaaaaaaga acacgaaaga
     2221 tttagacttc cctgcctatt cggtaagtaa taaagcaggt ttaatttctc aaacagaaca
     2281 gtttgatggt agtaggttgg atgatttaga aaaaacaaat tataaattcg ttgaaccaaa
     2341 tgaattcgcc tataatccag caagagtaaa cgtaggatca attgcattta acaatcttgg
     2401 tatgacggtc attgttagct ctctttatgt agttgtgaaa atgagcgagg acttagataa
     2461 tgagttcata cttcagttta tcaagtcacc aacatttatc aaagaggtca agcgcaacac
     2521 tgaaggaagc gtacgagaat acttattcta tgagaatttc gctaatatca agtttccttt
     2581 tactaggaat aaagaggaac aacaaaaaat cggcgctttc ttcaaacaac tcgacgacac
     2641 gatcgctctt catcaacgta agttagattt gctgaaggaa acgaaaaaag gcttcttaca
     2701 aaagatgttt gtttagggag ggaagataaa tgaaaattag tcaccgtgag gaagcggaag
     2761 tcgaagaaca gttgatccgt gtattggggg aagggcataa tcagtggacg tatcgtcccg
     2821 atctaaaatc agaagaagat ctttgggtca atttacgcca aaaaatcatc tcaaataatc
     2881 aagcagaact gaatgatttt cctttgaccg ataaagaatt tgaaacgatc aaaacagaat
     2941 tgttactacg aacgaaaacc ccctttgatg ctgcgaaatg gttaaaaggt gaaaatggaa
     3001 tggctcggat cacgattgaa cgggaagatc cgcaactggg ttctgtctcg ctcattcttt
     3061 attctaatca agacattggt ggaggaatct ctacctatga agtggtccat caaattgcaa
     3121 aacgtggaag taacatagaa gcacgggatc gacgttttga cgtcacctta ctgatcaatg
     3181 gattaccgat tgtccaaatc gagctgaaac aagtaaccgc aaaagatagt ttttatcagg
     3241 cgtttaacca aatcaaaaaa tatgcagaag aaggtatgtt tcggaacaat atcttttcga
     3301 ccttacagtt gtttgtggtt tccaatgaac aaaccactcg gtattttgca aatgccttac
     3361 caaaggattt gcatcctaag tttttattta gttggcgaac gaaagataat aaaaaggtag
     3421 aaaacttata tgagttttgc aaacaagtgc taaatatccc ggatgctcat cgcttgattg
     3481 cggattatac gattgtcagt gaagatcaag acaataaaat cctcatggtg ttacatcctt
     3541 atcaagtaca tgcgattcaa gccttgttta ttgctgcaaa taaacatcaa tcaggatatg
     3601 tgtggcatgc gactggttca ggaaaaacgt tgactagttt tgtatctact aagcttttag
     3661 cacgaaaatc ggggatcgat cggacgatca tgttagtgga ccgcaaagat ttagataatc
     3721 aaaccaccac agagtttacg aaatttgctt cggaatttaa tactgggatt tcttccggga
     3781 acgccaaagc gaatagcttg atcgtgggaa caggaagtgc caaagagtta agtgaaactc
     3841 tgttggcaga tgccaatgca aatgttgtga ttattacaac ccgtcaaaag ctagatgcgg
     3901 cgttgaaata tgcaaaaaaa caagaagaga aaaaaggaac caatcgcttc caaaaattaa
     3961 tggggcaaca tatcgtgttt gtagtagatg agtgtcatcg agcgttaagt gctgaaaata
     4021 tggaagaaat caaaaaaatg tttccgaaat ccacttggtt tggctttaca ggtacaccga
     4081 ttttccctga gaatcggaaa caagccaaag ggcaattggc acgtacgacc catgatcagt
     4141 atggagaggt cttgcatacc tataccatca agaatgcatt agaagatggc tcggttttag
     4201 gatttcaagt agagcatgaa aacaccgtgg agccgacttc attagagaat aaaatctatc
     4261 ggaaattaaa agaagtagaa acatatgcag agtattcacc ggaacagatc aatcgtatga
     4321 ttgatcaaat ggagcctgtc aaaaaagaaa gttatttgga ccctactgtt tttgaagcag
     4381 atgaacatat ccaaaaagtc attcataaaa tgttccgtcc agacaatgcc tatacaaaat
     4441 ttgattttcg aaatggccgt ccaacgaagt ctgcgatctt aaccacgagt tccattgaca
     4501 tggccaaaaa gtattatcga gcgatcaaag aaatgacgaa agaacctgac tggttaacaa
     4561 aagagtttcc taatcagccc attcgtgaag gaagaacgat ggaggatcct gattttccac
     4621 gaatcgctat tacctattca ttagaagaaa acagccgaga ttcaagtgtc caacaagaag
     4681 aaatgcaaaa aattattgag gaatataatc agtattatga tacttcctgg tcattagcag
     4741 acattgagcg ttataacgga gacatcaata atcgtttggc acgaaaaaga gcggaattca
     4801 aacaatttgg gaaacaaatt gatttagtga tcgtagtgga tcgtctattg acaggttttg
     4861 acgcacctac gattcaaacc ctatttgtgg atcgtaatct agagtatgca ggattgatcc
     4921 aagctttttc acgtacgaac cgtacgtatc cagaaaaaac aaaaggacta attgttactt
     4981 tccgaaagcc agccacaatg gagaagaatg tcgaggatgc gacaaaactg tattcagaag
     5041 caaaagaaga atcgggtttg atttatccaa gttatcagga atccaaaaaa agattcaagc
     5101 aagcatatgg taagttaaaa gaggtttcat ccatggaatc cattgatgaa catacgccac
     5161 ttgaaatccg tatcgagtac gtcaaagcat tccaagaatt gaatcgtgct tatgaatcgt
     5221 tagtgactta tgacgattac aatgatgaca tggtgaaatc ttcaaccttg aacaatcaaa
     5281 ttcaactttt ggaagaacaa attggcgtgt acgagaccgt gaagggttca ttaatagagg
     5341 aagaaccaga aaaagaggga gaagattttt ccacgatcga attttatagt gaaaatacca
     5401 cgaagttgta tgatattgat tctacttata ttgatcaatt actaggaact tatgcggcga
     5461 atagttcaga tgcacgagaa gaaattgaaa aagctttggc taaattgaat aaaacagaag
     5521 gagtcaaaga agtttaccga caaattttaa atgcgtttga tgtggggact ttagatagta
     5581 atgaagatat ctttgtcatt aaacgtcgct attttacaca agcgagtgat gatttgattc
     5641 atgtcttttc caatgagtgg tttgtttctg agaaagaatt acatgcttct aatctgcaat
     5701 atatgccagg tgaagatcca attcctaata tgaaagcaat tatcaacagt aaggattacg
     5761 aaggatataa agccaaccat ccagaagcga agccatttaa atacccacaa gaaatgaagc
     5821 gagcatggcg taaaatgtta gatgatgagc tgattccact tgaaaatgaa ttgaggtaaa
     5881 cgagaagagt taagaaaatt gtacgattca aagatggtag taaagtggaa gtgtagaaaa
     5941 tagggataga tgtcttttat ttgttcacat tttataacga agtatttaag acagctattg
     6001 aataatgttt cctccacctt tttcattcat ttaccccacc ccgttgttat atgaccgtgt
     6061 tttgagagga accaataaaa agcataggct aactaggggt tcctttttct tttgtttttt
     6121 tggaatccgc ttaagtgaaa tgtatctgaa atgaaacgga gataaagtga ttttttgctg
     6181 taatgacaag gttttagctg tttcagatac catgtaaccc aaaatagttt attgagaaaa
     6241 taggagagta tttgatacta actagtagga aaaaatgaga taaaaagtaa atggaatcat
     6301 ttcaggtaat ctcaaataaa aaaatataca gtcctgtggc actttaccta tgggactgta
     6361 tatatttttt actctcagcg ggcttgtttt gactccgtgt ctgactccgt cgtattgatt
     6421 ttaaatgata ttcgatgacc atttaacagg tcatttttga aatttttgtc tctatatgac
     6481 tcagtttgat aattattgat agatcaattt gggtctatat cgaacaaggg aataatccaa
     6541 taaaaaatga tttagaatat caaaataaca aaaaagagga ctacctgtat tagt
//
LOCUS       DACCXO010000135         6551 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.142, whole genome shotgun sequence.
ACCESSION   DACCXO010000135 DACCXO010000000
VERSION     DACCXO010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.142"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(1237..1902)
                     /locus_tag="IVY70_002555"
     CDS             complement(1237..1902)
                     /locus_tag="IVY70_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7959656.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2028..4064
                     /locus_tag="IVY70_002556"
     CDS             2028..4064
                     /locus_tag="IVY70_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP7959657.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4068..4706
                     /locus_tag="IVY70_002557"
     CDS             4068..4706
                     /locus_tag="IVY70_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7959658.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4734..5162
                     /locus_tag="IVY70_002558"
     CDS             4734..5162
                     /locus_tag="IVY70_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959659.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5619..6530
                     /locus_tag="IVY70_002559"
     CDS             5619..6530
                     /locus_tag="IVY70_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7959660.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2252 a          991 c         1212 g         2096 t
ORIGIN      
        1 actacctgta ttagtcctct tttttgacca gattttgacc agtctatttt acattctaat
       61 atattcatat gagctaattt tatgattaaa tggtaagtgt ttccttgttt gttttagttt
      121 aaactagtgt gtaatttaaa aatctatatg gaagggtctg tggggtaata cttaaataaa
      181 ggctaggaac ctgataacat caaggttcct agccttttta taataaaatg aagcgaaaat
      241 aatttctttt aaagagccga gattatcagc tatataagag tgaatttgat gcaaaaaaca
      301 gagccgaaac agtcttggtg ttcgtagctc tgcttatatg tttcgaattt aagacaaaga
      361 gttggcaaaa gatgttgata aggaagataa gataactgtg ttatataaac taggtaacta
      421 actgtaacaa gctaatttta agagattctt attagaagac gtttaggaat ataactaaga
      481 aaatgattat acaggtaatt gtcatttcaa taatgttttc taattttcgc gtctttgttt
      541 taagaaaaag gccagaatca gttggaaaaa agttcgtacg atagtgaaga ggggcaaaat
      601 ttcctactaa tattcccact aacaagagat aaaacggcca ggttggtgtg attcctctat
      661 aaaaaaatgc ccatgctacc gaaatcccat agattaacaa ctgaacaatt tttacaatga
      721 aaaaattgtt ctgccattac tcaaagtctc ttcctccttg tatcgtttat ttgtttcatt
      781 ttatcattat gataggcgct tgaacaatcc attttgtatt ttattgagta ctcttggata
      841 aactaccagc gattatcata tttatttatg ttactgtaag ccatcatata aaagtaaatg
      901 agcagatcat agaaatataa aaaagaacta aaattgatct aaattatgtg cagcttgact
      961 acgtaccaat ctatattact tactagattg gtcgttaatc tattcggatc gaatgagcaa
     1021 aatatttttg tgaatgtcca tccttgatta tgattccaaa aagaatcatt gggaggctgt
     1081 ggcgtaaata ttatactata agccagaaat acctatcatt ctgaactttt ttatttagga
     1141 actttcatct caaggctttt tcaattaatt tgtacccctg ttttttggat aagatcatgt
     1201 gcattgattt tttctatctc tacgttcatc tcctacttaa tgcctatctg gctcctgagg
     1261 gcttcttttt aaggcgtcgt catagtcttg gctgaggatt tcttggtaat ctgagggaat
     1321 ggcttcccaa gtgagcaggc gaacgttcat gacgtgtttt cctttgtgtt tgattacagc
     1381 atggtttttc tctttttcta cctctccagc gtgaaaagac agccatgtac catggccaaa
     1441 gatatcatta catacccaac gttcgatgga attgtccacg tatcctacag ggagtgtggt
     1501 atagccgatt tgtggtttca aaaaaggatt gaatcgatcg atcgtttcat ttttgtattg
     1561 ataattgata agaaaaagat agaccaaaca aaagacgagc agaaagaaag atagtgtctt
     1621 tttgagtgag aagggttgtt ttgatgaata agatttcttg ttaacaagag gttcatctgg
     1681 caatgttggt tctattgatt cggtagctga ttcaatagaa ggaaaatcaa tcggcagacc
     1741 taaagtatga tgtaaaagtg acagcatttc aaggtctggc attgtacgag aattttccca
     1801 actagaaatc gttttatcgg aaacaccaat tttttcgcct aattcttttt gcgtaagctt
     1861 acaatttctg cggtaagttc gtattttttc tccggtgttc atcttcttct cctttcgatt
     1921 ctacagtata tagatttatt ctacatgacc tatacacttg atagatacgt ttttttgatg
     1981 aaaaacggta tgattttatc aaagaatcat ttaagtaagg gagagtggtg ataatcaaaa
     2041 aaaagttcac agttttactc acaatcatag tgaccggtat catgagtggt tttgccttgg
     2101 ttagtataaa taaacaggta ctctatcgga caaatgaatt tttccatcaa acagatactt
     2161 atactccgat tcgattgcca caacatttta ttgggcaaga agaatatgag cagctaatca
     2221 tggtatttga aaatgtctca tcagaaactg agctttctta tatgaaaaaa acagtaaatg
     2281 aatccaatga agtagataca tcttggaact tcaagataca gccaaagctt gaaatgacat
     2341 tgtattatca aggggatgtc aaatcttctc acgagaaggt accctattct aatacctcct
     2401 ttcgcgtaac gtccattgaa gaatcgatca aaataaaaga ttttgaaggt gaatttttct
     2461 tgatgacgag agaaaaagaa gcctatgaac ggttcatttc actattcact gatcgattca
     2521 atcaagcatt tcacactgat tatgccacta atatttttga agactttgaa aatagctatt
     2581 cgtattcggt tatttcaaat acagacgtat ataattatcg agtgtattta actattggat
     2641 tgattctttt attccttttg ttatcatcct ttttcttatt gatgaataaa gaaatcagtt
     2701 tattgttgaa taatgacttt tcattacatc aagtattgtg gtgtttattg gggaaaaaga
     2761 aaatcattat tacattcata attgcaggaa tcattggtgg ttttattttt atgtattata
     2821 aggagcaatt tctttatttg ttccagatgt acgttttctt gttcagcgga atatatggta
     2881 tctctttgat ttctctttta ttagttgaaa aaatcgcttc gttaagagaa gcgttcaact
     2941 atatccaata cattcttttt acatttggat tgttgttttc tcttcttttg accgttgcca
     3001 atgtcgaatt agcaactatc gttttttctt cttcacagtt agctgtttcc catgaaccac
     3061 caaaagaaat cgctggtgag gactatcatg tattttatcc agtaaccatc gggaaaaacc
     3121 acgtggattt tatttattcc aatcgttttg catcagcagc agatcaggaa ttatatgaaa
     3181 ctttgaataa aaacggaagt atattagtca atgtatcaga ttacttgaat gaagaaaatg
     3241 aacagtttgg cagagggata aaaataaatc tgaattatct taagaaattt cctttgattg
     3301 atgtctctgg aaggttgatc caaataaccg aaaaagaaac gcaccctgtt atattgatcc
     3361 ctgaacgata tagacataca gatttaaaaa atgacctggc gcaaataaca gaatattatc
     3421 aagaaatcag tgaaaaagaa ccgaaatttc tatttataaa aaacaatcaa cccatttata
     3481 catttattcc ttctatcccc tggatcgaac attatccggt tgttgaaata ctgacattga
     3541 aaaacagtat gtatttggaa agaaatattc tcagcggaga aatctatcca ccgttaaaga
     3601 tcaagatagg ctctttgtcg aaagaaaggt tatttgagct gattgaagaa agtcagttac
     3661 gtgataactt gcagttatca tttccgtata ctcagactga agagatagct gtcaaagtgt
     3721 taagcagatc atttcattac ttgatacaga tatttttact cactttcttt tctttcttct
     3781 tgttgagcta tgtcatgtta tgcatatatt ttgtctacaa ttgtcggaaa atcgctatct
     3841 ttcgttcaag tggttattcg ctctttgaaa cgtataaaga tttcttcatg atgaacctca
     3901 tcaaatgggg aacgacaagt gtaatcttcc tttttcttat agaaagagag cctaaatatc
     3961 tttttaatat ttttttcttt agcttcattg attttattct ttcagttgtt tttattttgt
     4021 ctacagaaaa gaaaagtcag ttattgctga tgaatggagg ttaacaattg gagccgttga
     4081 tagtgataaa aggtgcgaaa aaaatcattc gaaacaaaac gattcttcat ttggatcgtt
     4141 tcactgcgga tgctggggat cttgtgactg ttgttggaga aagtggagca ggtaaaagta
     4201 cgttattgaa tatattagga ttgaatgatc agttcagttc aggacaattc tatttgttcg
     4261 atcaagagac gaaacaatta actgggagag agaagcgctt gataaaaaga aataccatat
     4321 cttttctttt tcaagatttt gggttgattg aatatgaaac aatcgatttt aatttagata
     4381 ttggattgaa gttttcgaat ttaaatgttt cagataagcg aacagcaaag tatgaggcat
     4441 tgaaaaaagt aaatataaac aagaagctaa gtacgcctat ctacacatta tctggtggtg
     4501 agaagcaaag aatcgcctgt gcgagagtct tattgaagaa tagcaaaatc attttagccg
     4561 atgaaccgac tgggtcattg gataaaagaa ataaggataa agtagttgaa atcttatcgc
     4621 aacaagcttc ccaaggtacg ttagttatcg tagtcacgca tgatccatat ctgaccaaaa
     4681 taggaaacaa gaagatcatg ctttaaaaat caaaaaggaa aggatgtcgt tttatgaaaa
     4741 aagcagttat tatttgtgca ctagggatag gttttgttgg aggaaacgct atgtcatctg
     4801 aagtggaagc aaattcagtt cacggtgaaa tctattacaa atctttaaag ccaggagaga
     4861 ttcattttga tgctaaaggt aatattatta agtcaaattt accagtagac cgggaaattt
     4921 ctagaacagc aattaatcat agctctggaa gatgggttta ttatagtgat acaacttgta
     4981 cgagaaaaac aggtcattct aaccactata gtcgtgtcta taatcgtcac ggtgcaaaag
     5041 cgaaagttgg aaactctatt cgatcagcaa atgcaacaaa aggagtatgg gcctatgcaa
     5101 gtgcgacagg aaatagaact gacacattct catgttggta caatccaacc ggttggtatt
     5161 aagtatccaa aagtttatat aaaatagaaa aaacatataa tgaaacagcg ttaaaagtta
     5221 tttttggtag ttttaatata ttcattttta gttgtataaa aatatatcat aataaaaaca
     5281 acaaaaaaat gtctggaaaa tcgaacataa aagtgtctga tttgaatatc ccagcagcgg
     5341 aagaagaaaa acaaaatcga taacatttgt tctaagaatt agttacgtta aatacggaac
     5401 gcaagataga cttgaaaaac ggtaacaaaa gaagtagtat attaaggtgt gatgtgctac
     5461 ttgacattta ttctgctgag cgaactttct ggattgtgta gccttatatc tattcaggac
     5521 gtatcgagca gattagtaaa ggtgttcttg atgattttca tcttgaacac ctttgttttt
     5581 agcaattcgc aaaaaacgaa aagtaggaga attgataaat gaaaaaaaga tttttagcac
     5641 ttgccatcgt actaggcact ggcttattgt caggctgtac gaatgccggt gaaaaaacag
     5701 ccgttagtta taaaggcggt acaatcagcg aacaagaagt aatggatagc ttgaaaaaaa
     5761 tgcaaggagc agattccgct gtgcaacagt tgatcgttta tcaagtattt gaagataaat
     5821 atggagatga cgtatcaacc aaagaaatcg attctcaata tgatcaaaca aagaaacaat
     5881 tgggtgattc atttgattca caattgaaat ctgcaggtta cacagaacaa acattcaaag
     5941 acagtatcaa acaaagtcta gcctttcaag aaggtctaaa gaaacatatc aaattgaccg
     6001 atgaagattt aaaaacggct tgggaatctt tccatccaga agtggaagct caaattatcc
     6061 aagtagcaag tgaagatgat gcaaaagatg tgaagaaagc tgctgataag ggagacgatt
     6121 tctctaaatt agccaaagac aagtcaactg atactacgac aaaagaagat ggcggaaaag
     6181 tgaaatttga ttccactact acaacggttc cggcagaagt caaagaagca gcctttaagt
     6241 tgaaagatgg tcaagtttct gatgtgatca cttctaccaa tgcttcaact tatactacgg
     6301 aatattatgt ggttaaaatg gtgaaaaacc aaaacaaagg caacgacatg gataaataca
     6361 aaaaagaatt gaaagaaatt gcaacagaca caaaattaag tgacagtacc ttccagaata
     6421 aagtaattgg cgaagtattg aaagacgcta atgtaaaaat caaagacaaa gactttgaaa
     6481 atgttttgag tacattcact tcagattcta gtacagcaag tagtaaataa gcggtataaa
     6541 taaaaaatat g
//
LOCUS       DACCXO010000136         6446 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.143, whole genome shotgun sequence.
ACCESSION   DACCXO010000136 DACCXO010000000
VERSION     DACCXO010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.143"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(99..1229)
                     /locus_tag="IVY70_002560"
     CDS             complement(99..1229)
                     /locus_tag="IVY70_002560"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP7959661.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IVY70_002561"
     CDS             complement(1878..3080)
                     /locus_tag="IVY70_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7959662.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IVY70_002562"
     CDS             complement(3140..3343)
                     /locus_tag="IVY70_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP7959663.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IVY70_002563"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IVY70_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IVY70_002564"
     CDS             4880..5692
                     /locus_tag="IVY70_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7959664.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1938 a         1315 c         1009 g         2184 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaat
//
LOCUS       DACCXO010000137         6194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.144, whole genome shotgun sequence.
ACCESSION   DACCXO010000137 DACCXO010000000
VERSION     DACCXO010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.144"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            233..646
                     /locus_tag="IVY70_002565"
     CDS             233..646
                     /locus_tag="IVY70_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP7959665.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(1104..1379)
                     /locus_tag="IVY70_002566"
     CDS             complement(1104..1379)
                     /locus_tag="IVY70_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959666.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            1833..2039
                     /locus_tag="IVY70_002567"
     CDS             1833..2039
                     /locus_tag="IVY70_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959667.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            2235..2402
                     /locus_tag="IVY70_002568"
     CDS             2235..2402
                     /locus_tag="IVY70_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP7959668.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            2428..2772
                     /locus_tag="IVY70_002569"
     CDS             2428..2772
                     /locus_tag="IVY70_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP7959669.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            2777..3058
                     /locus_tag="IVY70_002570"
     CDS             2777..3058
                     /locus_tag="IVY70_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959670.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            3161..3475
                     /locus_tag="IVY70_002571"
     CDS             3161..3475
                     /locus_tag="IVY70_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP7959671.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            3453..5147
                     /locus_tag="IVY70_002572"
     CDS             3453..5147
                     /locus_tag="IVY70_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP7959672.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            5167..>6194
                     /locus_tag="IVY70_002573"
     CDS             5167..>6194
                     /locus_tag="IVY70_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP7959673.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGV"
BASE COUNT         2167 a          903 c         1275 g         1849 t
ORIGIN      
        1 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
       61 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
      121 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
      181 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
      241 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
      301 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattataac
      361 ggatatgccg aaggcaccga agcatggcaa taaggcagaa gacgcgatta ttcagatgat
      421 ggatatagaa acggagagag atgcgattct agcggctttg atggctctta gtctgattag
      481 tcgtcagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acagaattag
      541 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
      601 gtttgcagaa gcatataaac acggaaaaat aattgcttat aaataatttg gcggtttttt
      661 ggcggaatga tggcggtttt tagacattta ccagtgttat tatggtagtg tcgaaagatt
      721 aggaaacagg atcgacaaaa taaacgaagg gaggcaatct ccctcatcgt tttaaacttc
      781 ttcttagata gacagcaact aattatgaag aaaaggatgt gaattcaact cctcctaaat
      841 tcttcatcgg ttgctgttta ttttgattag acagcagcac tcacaaaact aaacatgttc
      901 ttgtcgtctt tcaactgctg ctgtctatta atttatgtat tggagggaag aaaaatgaaa
      961 ttatcagtag aaggcactcc agaagaaata caagaattgc tccaagctat tggtagtagc
     1021 aaggagcaaa aggtaagtgt ttctaattca gatattgacg gtatttgcaa ttgtttaaac
     1081 agagaaaaaa tatctaatcc ttactaagag aaaatatgtt cgttaatgaa tttccaattt
     1141 tcctctgtag ctctaccttg ataatttttt tgcagttcac agatgaataa ggaatcgtta
     1201 ttatcaagta ctgtatttaa tttttcacac atttgatttg gattcaaatt agttttaact
     1261 aaccaaaaag atttttgtag ctttatatat gccccaaatt cttttattat ttcaaatact
     1321 ttatcatact tttgccctgg gttattaaga tcataactaa tcatgtaggg tttatacatg
     1381 ttgtcttgca catccttgtt ttatatttca gcggaccact cgctgataaa taaaattata
     1441 cgcttagtat ttattttcac aatattaatt tatcgtaata acttttgagt aaatagaaca
     1501 aaaaacctgc actagtttcc gctagtacag gcagtgacta tatcggttga taatctagca
     1561 tatattaaaa ataattgcaa gaaaagaaat ttatagtggt ttataccaaa ttatcaaagc
     1621 aatgttattt tgttgctgtc tattgttttt taattattca cacgataact aaaggtgggt
     1681 gaagagaaat gattccatta ataatttcaa tttttgcgct ctgtcttaat gtctatatga
     1741 ttggatttaa aaatgggcaa aataaaaaat agtagcagcc aagaataatt ttatagtgtc
     1801 actgtggcgg aaagggtaga cgctaagcat gtgtgctagg tcaatgcttc ggcaaccatg
     1861 caaggttcga ttccttgcca gcgacattaa atgcctatga cggttacgac taccgaaaaa
     1921 agatcgttaa gaagctatac ggtgctacgt acggcaatgt agtaagtgtg ctatctgtac
     1981 accaccaagc ttcggtcact gtggcggaag tagaagacgc agcggtaaat ggcgagtagc
     2041 ctcgtgagag cctggtaagt tctcgtgagt ggtgcaatcc cactccagcg actttaagca
     2101 accgaggcat cggcggttta aaaatatagg ggtgcgcaat ttcgtacgcg ttttgtgcat
     2161 cgtgcaagtt actattacat attagatcac tctttgagtg gtctttttat ttttgcacaa
     2221 aggaggaaac aacaatgtat agaccacaat acttagaaca gaagtatgaa gtaatcactg
     2281 ttcataaagg taatggcgaa aaagtatatg agtatagaag accaataaag agcgatacat
     2341 ataaacgaaa ggaaaacaat gaagttattc cattgtatgg caaaagaata gctaagcatt
     2401 aaataagatt gcgaaaggag acggaacatg accgaggaat tctatagatg gctattacag
     2461 ttgacaagag aagatcgttt ggttaagttc tatcagtctc ctaaatggcg caggcttaga
     2521 gagaaagcga tgaaacgaga tcactatgaa tgccaagagt gtagaagact aggtaagtat
     2581 catagagtag agaacgttca tcatataaag gaagtcaagg atagacctga cttagcttta
     2641 gatttagata atcttatttg tttatgtgtt gaacatcata atgaagttca tggcagatat
     2701 cttacagcat tagataaaca agagaagaag atagaaagct ttgctaactt cgatgcaagt
     2761 gaaaggtggt aagtgcatga tcatcaatga taatggcaga gagtatgata cagaaaagat
     2821 tgaagagtat tcatcttata ctcagggatt aattaaacgt ttgatatacg ttcgctatgt
     2881 aggtattagg gatctgttat cagataactg ttgtagtaaa tacaaagtga atcaagtaag
     2941 agaagcgttg aataaagata ataacgtcga aagaataaaa aatgtttttg gatatagtat
     3001 tgaagagatt aattattaca ttgacttcgc tgaagctttc attccgatgg tgagataacc
     3061 cccccttaaa ataaatcgca aattttttgg gggtgatgaa acggaggggg ctgtcaggaa
     3121 aagagatttt ttcgaacttt atcatgaaag gagggctaaa atgtttaaaa acgaattgtc
     3181 tcaaaatcgc tacagagaaa aattacgccg ctctttaata agccaattgg aaagtcagaa
     3241 aacaaatatt gagccattct tagataatgt tgatcgttat atcagtttat gggaaacggc
     3301 gatatcactg gaagaagata tatccgagaa cggcattaga ctggagaatg gtaaaaagaa
     3361 tgaatcagta gcgttgcttg tttctgtcaa caaacaaatg ggattgatgt tggataaact
     3421 tgccattact cctgaattgg taggtgaagc aaatgaatca attcctgagt tataagcata
     3481 ttgaaaattg gttcaaagct atagaagaag gcactatcaa ggtatgcaaa gagcaattat
     3541 tgctaaaaaa ttatctagaa gaaagagtct ttactagaga agatatttac ttcgataagc
     3601 agatggtaga ggattcaatc aatataccag cacaatactt tccattcgaa ttaattccgt
     3661 gggaaaaatt tctacaatgt tttatttatg gtgttcgatg gaaaaaagat aaaacactag
     3721 tgttcaatag atatctttca ttaatgggac gtggtaatgg taaaactggt tttgcttctt
     3781 ggaacaactt ctttctacta accgctaaac acggtattaa aaattatgat attgatatct
     3841 atgccaataa tgaaagccaa gcaaagacta gttttgatga tgtatttaaa gtaattaaag
     3901 atcatcctga tttagataaa aaagtattta aagctacgaa ggaagttatt caaaatatcg
     3961 ctacaaacag caaacttcgt tataacacgg caaatgctag aacaaaagat gggaagcgac
     4021 caggtgcaaa ccgctttgat gaaattcacg aaaatgaaga ttattcaatg ataaatgtgg
     4081 ctacttctgg tggtggtaaa attcgagatt atagagaatt ttatgataca actaatggtc
     4141 atgttcgtgg tggtccgctt gatgacatta tagaagaatc aaaaatgatt ctttctggag
     4201 aacttggaat tgacaaggat ggagcagaat tttctagttt gtttccattt atttgtcgct
     4261 tggataacga taatgaagtt gatgatcccg acatgtggga aaaagcttgt ccaactatta
     4321 attacaatgc agatttaaaa cggaaaatgt ttcaagaata ctctcaaatg caacgtaatg
     4381 ctggtttaag acttacgttc atgaccaaac gaatgaacag acctatggaa gatacacgat
     4441 ttgctgttgc ttcatatgat gatgttctgc atacgaaaga aaaagaattt cctgaaaaaa
     4501 tggatgaagt gataggaaca gtcgattttg ctgatagacg agattttgcc agcgttgggt
     4561 tgctaggaaa atacgataaa gatgtttatt ttacacaaca tacttttatc cacgaatcag
     4621 cccttcgatt acaaaacatc aaacgagagg ttatagatat ttctatagat caaggaaaat
     4681 cacagatcgt tcatggaaaa aatatagaag ctgattatat tgtaggttgg tttcttgaaa
     4741 tgagtaataa atattatatt aaaaaaatcg ctatggatat gtaccgtgca aaaatattga
     4801 agcccgcttt agaagaagca ggttttactg tggaaattgt tcgaagcgga tctgttacac
     4861 atggtatgtt aaaagatctg gttgatgacc tttttattaa tcaacgttta ttttttggtg
     4921 acgatgcgat tatgcgttgg tattgcatga atgtatatga agagcatatt tctaatggaa
     4981 atatacgcta tgaaaaaata gaacctgaaa ctagaaaaac ggatggcttt ttttcattcc
     5041 ttcatggttt gaatttttta gatgatattt atgattctgc tcctgtaaca gtcacaaata
     5101 gctcagtaga aaatacagga actggattta ctcctctagt attctaactt gaaaggaggt
     5161 gagaaagtgg ggatttttca aaaggcggta ggatacttca caaaaaaagc aacggttcct
     5221 ttagaagaat acttttgtaa attgcaagtt gattttgtgt atcgaaaatt tgcgattgaa
     5281 acttgtattg atttgattgc aaatgcgatg agtaaagcgg aattcaagtc atatgaagat
     5341 ggaaaaaata aaaagaatga tctttactat aggctgaatg tagctcctaa taagaaaaat
     5401 aatgcaacag aatttagaaa aaaactgatc aggagattaa tattctacaa tgaagtattg
     5461 atcgtttctc cgtctaataa ttctagcgaa atatttattg cggatagttg ggatgtcaca
     5521 gaatatgcat tgaaagatga tgtgttttct caagtgcaaa ttaacaacat agtccttgat
     5581 agagaatttc tagaaagtga tgttatctat ataaaatacg cagatcaaca aattaggcaa
     5641 ctagtcgatg cgtattatca agcgtatggg aaactcattt ctagtgccat gaatgtttac
     5701 aagcgctcta acgctcgtag atacgtactg aaagggaatt tattccgacc gcaagacaat
     5761 acaacacaag atcaaatcaa taaaatgatg acatcacaat ttaaggcttt tatggaagct
     5821 gataatgcag gtgcggtatt tcaattacaa aatgagtaca cattagaaga tttcagcgga
     5881 aacttccaaa gcaattcaag agatataaaa aacttaatag acgacatctt tgagatgaca
     5941 gcagcagcgt ttcacgttcc gaaaaaccta ctaaagggag acatgagtgg gttatcggat
     6001 caagtggacg cttttttaat gttcgaaatc ataccgattg ctgaacttat tcaggatgcg
     6061 tttaacgcta gtctctatga agtagaagaa tacttgtcag ggaattttgt acgtgtggat
     6121 acaactatga tcaagattac tagcttcaaa gatttggttg acgctattga tgtaggcatt
     6181 agaaatggag tatt
//
LOCUS       DACCXO010000138         6173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.145, whole genome shotgun sequence.
ACCESSION   DACCXO010000138 DACCXO010000000
VERSION     DACCXO010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.145"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            725..811
                     /locus_tag="IVY70_002574"
     CDS             725..811
                     /locus_tag="IVY70_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP7959674.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IVY70_002575"
     CDS             complement(932..1621)
                     /locus_tag="IVY70_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959675.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="IVY70_002576"
     CDS             complement(1628..1906)
                     /locus_tag="IVY70_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959676.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="IVY70_002577"
     CDS             complement(1866..2780)
                     /locus_tag="IVY70_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7959677.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IVY70_002578"
     CDS             complement(2762..3142)
                     /locus_tag="IVY70_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP7959678.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IVY70_002579"
     CDS             complement(3592..3879)
                     /locus_tag="IVY70_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP7959679.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IVY70_002580"
     CDS             complement(3899..4123)
                     /locus_tag="IVY70_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP7959680.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4793)
                     /locus_tag="IVY70_002581"
     CDS             complement(4578..4793)
                     /locus_tag="IVY70_002581"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959681.1"
                     /translation="MEKMVAAMEMKKEILMTKKHLLFGSILLGILSFSPMLLSVANKG
                     NKKGELKLNPNYYNSQNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="IVY70_002582"
     CDS             complement(4763..5296)
                     /locus_tag="IVY70_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP7959682.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IVY70_002583"
     CDS             complement(5317..6159)
                     /locus_tag="IVY70_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP7959683.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACCXO010000139         6098 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.146, whole genome shotgun sequence.
ACCESSION   DACCXO010000139 DACCXO010000000
VERSION     DACCXO010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.146"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(129..509)
                     /gene="yajC"
                     /locus_tag="IVY70_002584"
     CDS             complement(129..509)
                     /gene="yajC"
                     /locus_tag="IVY70_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP7959684.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(606..1751)
                     /gene="tgt"
                     /locus_tag="IVY70_002585"
     CDS             complement(606..1751)
                     /gene="tgt"
                     /locus_tag="IVY70_002585"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP7959685.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1969..2667
                     /locus_tag="IVY70_002586"
     CDS             1969..2667
                     /locus_tag="IVY70_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP7959686.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2835..5474)
                     /locus_tag="IVY70_002587"
     CDS             complement(2835..5474)
                     /locus_tag="IVY70_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7959687.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
BASE COUNT         1714 a         1334 c         1113 g         1937 t
ORIGIN      
        1 tttatatgac acacccgatt attcaatatt gagcaatata tcccatgctc ttatctcttt
       61 cttttttgaa taaaaaatga ggtaggacat ctttctgccc tacctcgtct ttaaataagc
      121 tttgatactt atttttcttc cttctcttct gtctcttcca gcgtttctgt tgtttcagtt
      181 tttacttcaa ctgtctcagc tggttcttct gttgtttcaa cagtagtcgt tgcatcattt
      241 gttacgacag tccctggttt gaccgttttg attgctgcac gatcgtactc aaggaagatc
      301 ccttcgcagt ctaaaacaac tgtccgtttg tcattgtcga tttctgaaac aactccatgt
      361 aatccgccga tcgttacgac tgaatcacct ggtttcatgc tatctaacaa gttttgacgt
      421 tcttgctgtt gtttcttttg cgaacgtgac ataaaaaacc acattgctaa tagcgcgata
      481 aacatcacaa gcattggtaa tgttcccatt tttttcacct cactaattct gtatctacaa
      541 aaaacacttt agcagaaaaa tgctgataac actagttttt tacttaattt ttaaaagatt
      601 ttcctttaga agctttttgc gttttcttta ttgaacccgt attcttcaaa aaaggcttct
      661 ctaaattcaa gcaaattatc atccatgatt gcttgacgta cttgtttcat tagattcaac
      721 aagaagtata aattgtggta agaagtcaaa cgaatcccaa atgtttcatc acatttgatc
      781 aaatggcgga tataggcacg tgtatagttt ttacatgtat aacaatcaca tttctcatca
      841 attggacgga aatcatgggc atatttcgca tttttcacta ctagacggcc ttgagaagtc
      901 atacatgtgc catttcgagc gattcgagta ggcaagacac agtcaaacat gtctactcca
      961 cggataacac catcgatcaa tgaatcagca gcaccgacac ccatcaagta acgtggtttg
     1021 ttttcgggga tcaacggtgt agtgaattct aatacacgat tcatttctga tttaggttcc
     1081 ccgacagata acccgccgat tgagtaacca gggaaatcca tcccgactag atctttcgcg
     1141 ctttgacgac gaagatcttc aaaccctgct ccttggatga ttccaaataa tccttgacgg
     1201 tctggattag catgagcttt caatcctcgt tctgcccatc gagacgtacg ttctactgat
     1261 tttttcacgt agtcgtaact ttcatcgaat ggtggacatt cgtcaaaact catcatgata
     1321 tcagagccca atttattttg gatgttgatt gctttttctg gtgataaaaa catacgcgaa
     1381 ccgttcaagt gatttttgaa atgcacgcct tcttcttcga tattccgcat atcacttaat
     1441 gaaaaaactt gaaagccgcc tgaatctgtt aaaatcggtt gatcccagtt cataaattta
     1501 tgcagtccgc cagcttcttc tactaagtct tcaccagggc gtagccataa atgatatgta
     1561 ttacttaaaa taacgccagc acccatttct ttcaactctt ctggggacat tgtttttact
     1621 gtagctaatg ttcctactgg catgaacatt ggtgtgggga aagttccgtg tggcgtgatc
     1681 aattccccta aacgcgcgcc agtatgtttt tctttcttga ttaaacgata gcgaatggca
     1741 ggttgcgtca ttcatttccc tactttctat ataaaatatc ttgacaactc ccaccatcat
     1801 aatcgtttca agtgcttttt gcaagttccc tcggcttcct tctactttat ttaatattct
     1861 tttttttatc ctttttttat gaataacaaa aaaaagaaaa acattatgac agaaatattt
     1921 tttgatacat ggtatattgg tgttattaaa gaaaaggagg gaaaaatcat gcaatcttct
     1981 gcagaattaa aacgccaagc aaaagagagt ctaaaaggac gttggggaca agctgtttta
     2041 ctaaatctaa tccctacatt gattggtatt gctactattc ttcttattat gattcctatt
     2101 atcttgtttg ccaccaccat atatgatgga tcagcgagcg tctcagaagc gacaacttca
     2161 ggaggcggta gttcaggagg cggtggcgga attatttcca gcatcattag cgctttgttc
     2221 atgagcggta tttcttggac ttatcttgat attttacgtg gacaaaaaga tcagatcgaa
     2281 ccattcaaag atgcatttcg tggattcaaa ggtttgttta tcggcggagt gatcagctta
     2341 gctgtgctga tctcgatctt tacaacatta tggggtttgc tattagttat tcctggaatc
     2401 attaaaagct attcttattc tcaaagctat tttatttact acgatatcgt ccaagaaaca
     2461 ggacaaacac ctaaagtact ggatacgatc actgcaagtc gaaaattgat ggatggttat
     2521 aaaggacaac ttttctggtt agatctttca tttatcggct ggcatatctt agcattagca
     2581 actgctggta ttggttatct atggctcagc ccatacattt ctgcaacaaa agcagcattt
     2641 tatgaacaat tgccaaaaga tatttaaaag agagtgtgac ttgtgaccga gggggtggca
     2701 ggagaacacc gtttatcgag aggcagtaaa agaaacattc agcccattag caaaaaggag
     2761 gtgtgacaac attcatgtca cacctccttt ttgtatagtt ccagtaaaaa gaaaatcaac
     2821 ttagaaacag ttaatcattc ctcttcttct gcttccggta atcgatggaa cttgatcagt
     2881 tcattcaccg cgattggtac gatagataaa atccctacca agaaccagtg attcaaactg
     2941 atgtgtacta ggccgaatag ttcttgcgta aatggcagca aagcaacagc cgttgtaata
     3001 gctaatgcca gtaacgccat ttctaccaaa gctttattgg tcgctaaatt gactttgaag
     3061 atcgattggc tgctgcgtac attgaacaca tgcagaatcg atgaataagc taagatcaag
     3121 aatgcaacag tttgaccgac atgctgactt gcgtctaccc atgggctgat agtatcgaca
     3181 taactaccta aataataacc aaacaaggtt acgatggtga agacccctgc atttatggca
     3241 attttttgca ataaacctcg agcaaagatc ccttcatttt tcggaatggg tggctgatcc
     3301 atgatgccgt gttccgctgg ctctcttcct aaagcaaatc ctggaagtcc atcagcaact
     3361 acattgacaa acagtagttg cacagctgta aatggtgctc cccatcctaa cagcatcgca
     3421 atcaaaacaa taaagatctc cgaaatatta cagcttagca aaaagttgat agctttacga
     3481 atattctgat aaaccgaacg tccttgactg acagcgtcta cgatcgtcgc aaagttgtca
     3541 tccgtcaaaa tcatatctgc ggcactttgc gccacatctg ttcctgtgat ccccatggca
     3601 cacccaacgt tacttgcttt caacgctggt gcatcattca caccatcccc agtcatcgct
     3661 acgactgcac ccgtacgttg ccaagatttg acgatccgga tcttgtcttc tggcgttacc
     3721 cgagcataga cagagagagc tttgacacgc gcatctaatt cttcgtctga taattgatgc
     3781 agttctgatc ctgaaagtgc ttctgacgga tcattcaaga tacctaattc tttcgctatg
     3841 gcactggcag tcaccacatg atctccagtg atcatgactg ttttgatgcc agcctttttc
     3901 gctcgcttga ttgctccttt actttctgga cgtggtggat cgatcattcc gatcagacca
     3961 atcaaacgta agtctttttc cagtgcttca gaagaaatat cttgcggttc ttcgtcataa
     4021 gtagcataac caacagcaat cactctcagt gcccgtttac caaagcgatc attaacgatc
     4081 gccgcttgtt ctacatcgcc tgaactaaag cgcggagcaa gaacatcgaa tgctcctttg
     4141 gtgattgaga tgtatttttc gcccatttgg tgaactgttg tcatcatttt tcgttcagaa
     4201 tcaaaaggaa gctcgtttac tcgtggatat ttttcttcta attctgcctt tgtatgataa
     4261 ttctcttcaa cagcccttac gatggctact tctgttggat tacctttgat aacaaggtcg
     4321 tcatcttctt gctcgaccgt cacatctgta catagtgcag ccattttcaa tatttccatc
     4381 gcgctgttcg tcattgaatc ttcgatattc gttacttcat cttctttgct ccatacccgg
     4441 cggacgcgca tttcattttg tgtcagcgtt cctgttttgt ctgagcagat aacattagcg
     4501 gttcctagcg tttcaactgc tggcaactgt cggatgatcg catgtttcct cgccatcttc
     4561 tgcacaccgt atgcaagtgt caacgtaaca ataacagtca aggtttccgg tacagctgca
     4621 acagccaagg aaatcgaagt catgaacatc tcaaggagtg gttctccttg cagttcacca
     4681 atcacaaata ctaaagccgc agccgctaaa gcgatcatac tgattcgttt gcctagctga
     4741 ttcaatcgga cctgtaaagg tgttttttgc atagtatttt cgttcaaaag atccgcgatc
     4801 ttacccattt ctgtagccat gccaatggca gtgacgatcg ctttcccacg acctgcagca
     4861 accgtacatc ctttaaatac catattaagt tggtcaccta atgactcatc ttcttcagca
     4921 ataaatgctg catctttttc aactgcttca ctttctccag tcaacgccga ttcttctaca
     4981 cgtaattgtg atgcttcgat caaacgggca tcggcagaaa tagcatcacc ggactctaaa
     5041 acaagaatat cacccactac cagttgctct gactcaacag tggtcacttt gccgtctcgt
     5101 atgacagttg ccgtttgttt attcatatct tgcaaggatt ccagtgcttt ttctgcgttt
     5161 ccttcttgga caatcgccaa taccgagttg atcacaacga tagcgatgat cagcagtcct
     5221 tcaaataaat ccccatgttc tgtagcaaat gcggtataaa aggaaatcac cgctgctaca
     5281 agaaggatga tcgttgtaaa atcggacaag cttcgaaaga attttttgat catcgattct
     5341 ttcggcgcct catcgaattt attcaatccc tgcttttttc gccgttcatc cacttcttgt
     5401 gaagacaacc cagtatctgg atctgcttct gttaccttct ttacttcttc tattgtctgc
     5461 ttgtattctt ccatcctatt cctcctttaa aatcttcgta caaaaaaagg tacttatttt
     5521 actataacat ccttttttta tttacttcaa ataaagggct tatcgattat ttgttaagaa
     5581 agcgtaaaat ttaaagtgct tatatttgca aacgctacca aaggatgcta ttatatagat
     5641 gtaaggaagg aggataccat caaatagaca gaagaatttc tcgatagaac agtgtaagca
     5701 aacaaagttg ttagaagtac aggtttcacc cagacatgcg tgtagagtac aaacctataa
     5761 atagtgaatg cgtatggatg tttggatcac gaagtatccg aaacaagaag taatgcgata
     5821 cgtaacaatt gaataacgta aacgtctgaa aattgaatag gaacaagaga tgtttcaaat
     5881 gttgaagatg taacgaaaac aaaatttttg tttaacatga agaaattcaa caccgtgctt
     5941 tgcatgtatg atggaaagtt acaatcattt ctgtttattt gaatccttcc gaggtacaag
     6001 aaagccgagc ttatcgagag ctcggctttt tctttgtcat aaaatctttg taaagctttt
     6061 ttgttattca aaactagcgg cttttcataa ccttattt
//
LOCUS       DACCXO010000140         5877 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.147, whole genome shotgun sequence.
ACCESSION   DACCXO010000140 DACCXO010000000
VERSION     DACCXO010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.147"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            641..1375
                     /locus_tag="IVY70_002588"
     CDS             641..1375
                     /locus_tag="IVY70_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7959688.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1391..2560
                     /locus_tag="IVY70_002589"
     CDS             1391..2560
                     /locus_tag="IVY70_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7959689.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2576..3571
                     /gene="lacD"
                     /locus_tag="IVY70_002590"
     CDS             2576..3571
                     /gene="lacD"
                     /locus_tag="IVY70_002590"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP7959690.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3650..4576
                     /locus_tag="IVY70_002591"
     CDS             3650..4576
                     /locus_tag="IVY70_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP7959691.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4595..5845
                     /locus_tag="IVY70_002592"
     CDS             4595..5845
                     /locus_tag="IVY70_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7959692.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1967 a          955 c         1204 g         1751 t
ORIGIN      
        1 gtttgaaaaa tgctatttcg aggtagaagg atattatata agcacggttg actaaacgaa
       61 gtgataataa aaaaagttat tcaagaataa gaaaaattat gatacgaatt cagataaact
      121 ggagaataca gagaccttta aaagggataa gaaacaacat aaattcctct taagagagaa
      181 cgaaaggaca aagccagcaa cttgatacac gagctagtat tagaggaatt tattctcata
      241 gactcccttt tgaatacgtt tcaactttaa ttttcatgtt cataaatgat ttgaaaccaa
      301 agcaaatgcg ttgcactttt cctccgtctt ccgaaaatcg ttcaattcac tggtaagtag
      361 tcatttgata gtttgaggca aattgttgat tgatgaaggg taggagctaa aaaaagatac
      421 cagttgaaga gagaaaaaga aaaaaataga gggtgttctt gaatgaaaaa agttggaaaa
      481 ttatttttca tttcctttta ttcctattaa agtcagtgat gtcaatcata ttaacaagac
      541 taaaaatgaa aataaaaaaa agattgttct tagtattgac tcataaaaaa taccactact
      601 atacttacat tgtggttata gccagttagg agagacatcg atgcaaaata cctttcaatc
      661 aaaatcactt tattatcagc tggtagaaat attgaaacaa cgaatcgaaa acagtatggt
      721 cgctcatgac aagctgccat cagaaagaga gttgaccagt ttttatggcg ttagtcgcac
      781 gacggtgcga ctagcattgc aagaactgga aaaaatgggc tacatttatc gacaacatgg
      841 aaaaggaacc tttgtttcgg aattaaggac aaatgcgact gatctagctg gcgcatatag
      901 ttttaccgaa caaatgcggg caatgggcaa acatcctgaa accaagatat tggccttcaa
      961 gaagattgaa gcaactaaat atttagcgag tcatctgaat gttcaactag gtgagccttt
     1021 atttcaatta gaccgattgc gcttagctga tgatttgcca atgatgatcg agaaatccta
     1081 tttgccagtg aagctattta tgtccctttc tcaagaaatt ctatttctga aacctttata
     1141 taatttgttt tcagagaatt tcaatcaaat cgtacggttg gctgacgagg aattttatgc
     1201 cagcattgtc cagaaagaag atagtgagtt gctaaatatt ccagaaggat caccagtgct
     1261 ccatttagtt cgctcaacct ataatctcga gaacgaagtg attgaattta cattaagtgt
     1321 agcgcgggct gataaatttc attataagat caggcatctg cgttccacta actgaattgg
     1381 aggaaagaaa atgtttatgt tctcaaaaga agaactacga aagctaggag cagaaaatac
     1441 tacccgagaa attcgacagc aaccggaact gtggcatgag acgtttgacc gttatcaaga
     1501 aacagttgca gatttagatc gtttgtttac ggatgcgaaa caacatgctg gagagcgacc
     1561 aatccgagtc atttttacag gggccggaac ctcgcaatat gtgggagata cactaattcc
     1621 gtttcttact agtcaaggaa atacgaacca gtttattttt caaagtattg gttcgaccga
     1681 tatcgttgct acgccaaaag aatacctgat tcctgatgag acaacgatct tggtctcatt
     1741 tgcccgtagt ggaaatagtc cagaaagtct agccgcagtt aagatggcca atcagctggt
     1801 caacaagatc tatcatatcg tgatcacctg tgcaccagac ggattattgg ctacacaaat
     1861 gcctacaaaa aaagaaaatc tagttttact aatgccaaac aaagccaatg acgctggttt
     1921 tgctatgaca agcagtttca gctgtatgac gctgactgcg ctacttattt ttgatcaagc
     1981 aagcaatcag caaaaagaaa gatatgttca tcaattaagc cgactaggag aagaagtcat
     2041 ccaacgagaa tcagagatcc aagggtatct ggcatcagac tttcatcgca tcatttactt
     2101 aggttcggga agcttaggcg gattgactag agaagcgcaa ttaaaagttc tcgagttaac
     2161 tgctggaaaa atcgcaaccg tatttgatac atctttagga ttcagacatg gtcccaaatc
     2221 ttttgtcaat gagcaaacat tggttttcct gtttgtcagc aatgactcat atacgcagaa
     2281 atacgatgta gacatgctgg aagaaatcaa aaatgacggt attgcccaac gcgttgttgg
     2341 aattggtcaa aagggttcat tgtttagtgg tgattccttt gaattagttg cagattcgtt
     2401 actgccggat ggctatcttg ctttagcaga tatcatggta gcccaaacga tcgcattact
     2461 ttgttcggtg aaggttggta atacaccaga tacaccatcg ccaagtggaa cagtcaatcg
     2521 tgtggtcaaa ggcgtaacga tttatccata tgaaaaatag gaaagagtgt gaagcatgtt
     2581 agaatggtcg gaaaataaaa gacaagcaat ggaacgtcta tcgacgccag aaggcattat
     2641 taccgctttg gcgatcgatc agcgaggcgc gctgaagaaa atgatgtcgg ctttagggat
     2701 ggaagcgaca aaggaaaaaa ttgagcagtt taaaacacta atttcccaag aattgactcc
     2761 ttatgcctca gcaattttat tggatcctga atacggactg cctgctgcac agaagcgtgc
     2821 agcagcgagc ggtctgctgt tggcatatga aaaaactggt tatgatcaaa ccgtacccgg
     2881 ccgcttgccg gatctgctgt cggactgttc agttctgcga ttgaaagagt taggtgcgga
     2941 tgcggtgaaa tttttactgt attacgatta tgaagaagca gaggaaatca atcggcaaaa
     3001 gcaagttttt gttgaacgaa ttggaagcga atgcatggct gaaggactgc cattttattt
     3061 agagttattg tcatatgatg ctaaaaatgt agatacgaaa tcgctttcct atgccaaagc
     3121 aaaaccgcgc aaagtgatcg aaatgatgaa agaattctcg aaaacccagt ataaagtaga
     3181 tgtcttaaag gttgaagtac cagtaaacat gtcatttgtt gaaggaaatg caacagggga
     3241 gatcgcctat acacgtcaag aagctgccgc ttattttaaa gaacaaagcg atgcaacaga
     3301 tttgccgtat atcttcttaa gtgctggggt atcagcggca ttattcatgg agacattgcg
     3361 ttttgccaaa catgctgggg cacgattcaa cggtgtactc tgtggcagag caacttggaa
     3421 agatgcagtc gctcctttcg ttgaaaaagg agaagcggca actttagaat ggctgacaga
     3481 acaagggaca caaaacattc gtcaattgaa tgaagtgata agagaaactg ccatcccatg
     3541 gtatgagaaa gtaagtgaaa gcacttgtta atgtttctaa tatattagta gtgataagag
     3601 atatgtgtga ttataattta tcattattga aactgggagg atattttata tgaataaaag
     3661 attgttagat tattcagctt cagatttttt gaaagctaag ccgatggaat taaagcaggc
     3721 aattttggct tctgaaggta gaacaattat ggcagaaaat gtgccaagta gctgtcctta
     3781 catgggaaaa tcagtgacaa atgcagaatt ggagagagct gctggagctg atttaatttt
     3841 gtttaatgca ctagatcttt ttaatcctca aatttcaggt gtacctttaa ctctaaaaga
     3901 gaatccaata atctggatta aaaaagcttt aggaagagct attggtgtaa atttagaacc
     3961 tgtagacaaa gaagttttga tgagtgaaaa tcgaactgaa atttcaagtg gtagaatcgt
     4021 ttctcctgag actttaaaaa aggcaaatga attaggcttt aattttattt gtttaactgg
     4081 aaatccagga acaggagtat ctaatagaac tattgagaaa gcaataacat tatccaaatc
     4141 aatttttaat ggattaatta tcgctggaaa aatgcatggt gctggtgttg atgaaccaat
     4201 tatgaactta gatatagcaa aaagatttat taaaacaggt gtagatattt tattagttcc
     4261 tgctccatac actgttccgt atttcatgga aagtgatttg cgaggaatcg ttgattatat
     4321 aaaagatttt aatagaaata aatctattga ggaaaaagtt ctagtactaa cggctaatgg
     4381 aacaagccaa gactcttctg accaagaaac tattaaaaaa attgcattag catcaaaagc
     4441 atgtggagct gatattcaac acatagggga ttctttaaat ggtatttgtc ttccagaaaa
     4501 tatttttgct ttgggtcaag caattcgggg atatcgtcat caaattatta tgttaggaaa
     4561 atctaattat cgttaattaa ggaggaaatg aaagatgatc gacaaagttc aaaaaatagc
     4621 tgcaaaaatt agtaatcaaa gacatctact agctttaaga gacagcatgg ctgcagttat
     4681 gccattaatc attattggtt ccttttttat gttattagca aatttcccga tagaaggatt
     4741 tacaaaatgg ttatctgaaa ttggagtaca atcctttttg aacaaagcat cagattcaac
     4801 atttggtatt attggattag ctgttaccta ctctttggga cacaatctag caaagactta
     4861 tggtgtagac tcattatcag caggattatt atctttgagt ggatatgttt tacttacacc
     4921 tttattggat gcagatggtt ctataggatt tccaacaaaa tactttggta cctcgggtct
     4981 ttttgttggt attattgtag cacttgtatc tacagaaatt ttccggtggt ttgtacttaa
     5041 aaatattaca attaaaatgc cggcaagcgt tccaccaaat gtaagccgag ctttcgttgc
     5101 tattattcct ggcttttttg tcgttctact atggtttatt gttgttgtaa tttgttataa
     5161 acttggaatt gaaaatgttc atgctcttat tgcagataca ctcgcaaaac cactatcttt
     5221 actgacgaaa actttaccag gaattatatt ggttatactt attcaatgtt tcttttggat
     5281 gtttggtatt catggtgcac aggtaacggg gccaataatt gaaccgttat tattgcaaaa
     5341 ctctgatgta aatagaattg cttatcaagc tggaaaagaa cttcctaaca ttataactta
     5401 tgaattctta tataactttg ttttttctgg tggtgcaggt tgtttatttg cacttgccat
     5461 tttaacattt ttcttttcaa agagtcagga aaataaaact ttaggtaagt tatcaatcgc
     5521 tccagtaagt tttcaagtag cagagcccct tctatttggg tttccaacta tattaaatat
     5581 aaaaatgtta attccatttg taactgcacc agtagtaaca acattaatta cttatttttc
     5641 aatgagtatg ggattagttg caaaacctgt aggagcaaca atcccatgga caactccacc
     5701 gataattgcc ggatttttag cttctggtgg acgaataagt ggtgcagtga ttcaagtaat
     5761 aacaattgca ataaatgttt tgatatacta tccattcttt aaattagatg ataatgccaa
     5821 gctaaaaagc gaaaaaaatg attagaacta cctatatgtt tctaactgta gttaagt
//
LOCUS       DACCXO010000141         5842 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.148, whole genome shotgun sequence.
ACCESSION   DACCXO010000141 DACCXO010000000
VERSION     DACCXO010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.148"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(137..595)
                     /locus_tag="IVY70_002593"
     CDS             complement(137..595)
                     /locus_tag="IVY70_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP7959693.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(608..1933)
                     /locus_tag="IVY70_002594"
     CDS             complement(608..1933)
                     /locus_tag="IVY70_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP7959694.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1950..3362)
                     /locus_tag="IVY70_002595"
     CDS             complement(1950..3362)
                     /locus_tag="IVY70_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7959695.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(3379..4143)
                     /locus_tag="IVY70_002596"
     CDS             complement(3379..4143)
                     /locus_tag="IVY70_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP7959696.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4247..5122)
                     /locus_tag="IVY70_002597"
     CDS             complement(4247..5122)
                     /locus_tag="IVY70_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7959697.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5377..5461)
                     /locus_tag="IVY70_002598"
     tRNA            complement(5377..5461)
                     /locus_tag="IVY70_002598"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5425..5427),aa:Leu,seq:caa)
     gene            complement(5479..5550)
                     /locus_tag="IVY70_002599"
     tRNA            complement(5479..5550)
                     /locus_tag="IVY70_002599"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5516..5518),aa:Gln,seq:ttg)
     gene            complement(5560..5632)
                     /locus_tag="IVY70_002600"
     tRNA            complement(5560..5632)
                     /locus_tag="IVY70_002600"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5597..5599),aa:His,seq:gtg)
     gene            complement(5694..5778)
                     /locus_tag="IVY70_002601"
     tRNA            complement(5694..5778)
                     /locus_tag="IVY70_002601"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5742..5744),aa:Leu,seq:caa)
BASE COUNT         1785 a         1243 c          894 g         1920 t
ORIGIN      
        1 tcataggagc aacaaaaaaa ttcgataatt aacaaaaatc aaaatgatta aatctagtga
       61 ttgaaacata tcatttcaaa aaaattttgt aaattcaccc ataaatatca ccctatttaa
      121 cttttttcat cgtacactaa aattcaaaac aactatcttt aatctttact tcttggtttt
      181 gttttaataa atttccttca aaaatgcctg gagaaactac aatgatcacc gtatcttttt
      241 gtgcttgctc caacttcttc aaatctataa tagctaacag tgtcccttct tttacctcct
      301 ggttactttc aacccgtatc tgaaacggac ttccttcgag ttcaactgta tctaaaccca
      361 tatggatcag tatttttctg ccagattcac tttcgatcgt tatggcatgt cgggtaggaa
      421 atatattatc gattattcct ttaactggcg catggatata tccatcatga ttcgttatag
      481 cgtatccaac tcctatcatt tttgaagaaa agacctcgtc ttttactgct gttaatggaa
      541 taatttgacc actagctaca gcttttagaa tgtttcttgt tttaaagaaa tgcacttttt
      601 atcacctcta ctttaatgct ggccaatatt ttttgtttac tttgatcatt tcatctagta
      661 ttttttttgc gactctggca tttggtacgg tttgattcaa tgtaaatgct tgtaaagctt
      721 tttgataaga accttcaaag taagcatcta ctagtaactt ctctactgca acttgtgcct
      781 ccatcaatcc tttatgaaaa tcactgattg gttttcttag tgtaattggc tccacaccag
      841 ttgcaccaac atatgccggt atttcaacaa ctgcatctga ccgtagattc ggaatcgccc
      901 cttgattagg aacgattagc ataaagcgac gacgatcatc attcaacaag gaaatagcta
      961 tatcgacgat atattgtcca tgctccccaa aattaaagtt caatacttct tccacaatac
     1021 cgcggcgtat tttatcagcc atttctttgg tatttttctc tcgtccatcc atgaccatat
     1081 tagcacgtgt atactctttg tcagtatgct caacgaccat atcaggatat aagtaatact
     1141 ctaagtaatt attcgggata ttacttggaa aatttttcgt gatgactgac atcatttgaa
     1201 aggttttttg ccaagtttta tctcctatat taaaatcggc gacttgcatt tcttttgtta
     1261 ttagtttttc aataatctct ggcataattt ctcgttgcaa ttctttatca taaatcgatg
     1321 tataccagcc aaagtggttt aaaccataat acgtaacgat ccaattctta cgatcataac
     1381 cgtaattcac tgcaatcgtt tcttcaattg aaatagtcat atcacaggca ttaatcattt
     1441 ttacattcgg atatttacga cgaacagctt cagaaacgat cgactctgga tttgtataat
     1501 tcaaaatcca tgctttgggg gcatagtctt gtatatgatc gacaatttcc aataaacctt
     1561 tgattgagcg catcccataa gcaaatcctc cgagtccaca cgtttcttgt cctactaatc
     1621 catgtttaag tggaatcttt tcgtcctttt ctcgcatctc caatcctcca acacgaattt
     1681 gtgagaaaac aaaatcgcat cctgtaaaag caagctttgg atcatccgtt gcacgaatgc
     1741 gaatatttga aaaacctttt cttttcagca taaatttaac aatcaagtac atatccatat
     1801 ttctttcttc atcaatatca tataaacgta tttcagataa tggcaaccgt ttttcattgt
     1861 tcaaaatggc ttgaacgatt cctggtgtat acgtactccc gcctccagct atagtgataa
     1921 tctgtttttt catcatcatc ttttcctttc tattcttcta ataatttatt gaattctgcc
     1981 ataacataag tcacttctaa gccgatgata atttgataat ttttttcagt tggtttaatg
     2041 atcccgctta tccctaattg cttgattcgc tgttcctcga ccatggtggg atccactacc
     2101 tctacccgta ggcgtgtatt acaactatag acatccagga tattggcttt tcctccaaac
     2161 gcttgtagca tttttgttgc catatattca taatttttac ttgctaaccc taactctgtt
     2221 tcttctaagg ttacttcatt gccaaactct gccttagctt cacgtcctaa tgtctgaatg
     2281 tttctcttat tgatcaaata ataaaaagta acgtaataca aggcaaaaaa gaaaattccg
     2341 attggaagaa tcaaaatagc attggtagcg attcgaaaat tgatgagata atcaacaata
     2401 catgctccga atgtaaaacc aattcgtata ttgaataagt aacagacaat ccctgctaaa
     2461 ccggcaaaaa gtgcatggat cacgtacaac ataggagaaa gaaacatgaa actaaattca
     2521 acaggctcag tgatatttgc aataaatgaa gttcctgcac cgcttcccat gattcctttg
     2581 accatctctt tattttcttt ataagctgct ttatagatag ctagacatat agctggtata
     2641 ccaaacatca ttactacaaa aaaaactgat aaaaacagtc cagcctgagg atctccgttg
     2701 ataaagcgtg tcatctctcc tctgaaaact tccttgctag gagatgtata gcttcctaaa
     2761 tcgtaataaa tatacgtatt caacacatgg tgcagtccaa aggggatcag caaacgattc
     2821 aaaaacataa atatccccac acctaatgct cccatatcaa cgagcagttt tgcaaaatta
     2881 tcaatcccaa tttggatgag cggccagata aagcccaaaa aaacagctgc gattgtttgt
     2941 agaatcatca ccatagtcaa tgggaatttt tcgcccgcaa aataagaaaa aactactggt
     3001 aatttttgtt ctttaaagcg attatacgtc catgccgcta ctaaaccgga aataattcca
     3061 ccaaaaatcc ccatattcac ttctgggtcc ataatttcta agcctttttt caaggtagca
     3121 atcgctaaaa aaccagtaag tgcaggtatt cctttgtcct tatttttgac aaaaccgatt
     3181 gcaactccta tagcaaataa gatatccaag tttccaaaaa cggcatttcc agctgcacca
     3241 attactggga tgttcagcat atcgtcagct gataaacgca tgatcaatcc cgctaatggc
     3301 atcgctgcaa ctggaagtag catcgctctt ccaagcttct gcacatttga tttaacattc
     3361 atttttattc ctcctctatt aaatatcctc atatgttact aactcaatca aaggatcatt
     3421 tttcactttt tgagcaaatg ctgaatgaat taaaaattcg acttggtggg tacgaggata
     3481 cacaaaactt gaaccattta acaattcctg atccaaatac gctgcgtgac acatgacttc
     3541 gatcgtttta tcttctctca atatcatgag taaacttgcc aaatattctt ccacactcgt
     3601 attgatttgt cgtttttttt catcagtttc tcctacgaca tcgaaacaag gctcaaaata
     3661 atcgacatga cgaacttcat cttttcgttc aaagttgctc cgaaccggca aatcatattt
     3721 ttttgctaat gtaacaacta cctcctgatt ttcatccaaa gtatgagtat gatgatgact
     3781 gtctaaatga gttggtatga tccccgcatg ataaattttt tgaatctgag cattccattc
     3841 ctgatacagt tgatctttgt caatgttaaa tggatgctga taataagata aggatctgaa
     3901 ctctccgctt tcgataagag tatcgacagt tttgaggagt ggtttgccac acgtcaatgt
     3961 taaatggact cctacaccta gcctaggcat ttcctttctt agtttcacag catgctcgaa
     4021 acccggcata tttgccatca atgtagttga tgtcaaaatt cctcgctgat aagcatccat
     4081 aatcccataa ttcacaccat ggctatagcc aaaatcatcc gaattaaaaa tcacttttcc
     4141 catttcattc tccttctttt ttctactaaa ataggcgcaa ccaaataatc atagtccatt
     4201 tactatgtgt catttggttg cgcctataga tagttacgcc cttattttaa tagattcttg
     4261 ctagatgaat tgccaaatag atgatttcat tttcttgaat ggcaagttga aattgctgtt
     4321 cgatgatatc tttaatcttt agtgctgtac aatacgcttt gctatgttta aatgtttcag
     4381 taaaaaaacc aatattttct actggcgttt cttcctgata aatcaagcgt ttaaccagga
     4441 atttgatatg aaccattaag cgatgataag tcagtgattc tttatcataa acgtttgaat
     4501 aggatttttc tagatgggac aaaatcgtgg tcgtcatttc tactattttt aagctcttta
     4561 aactattagg ctctcctagc tcagcattaa tgatatgcat agtgatgaat cctacttcat
     4621 ttgaccctaa ctcggtttga tatcgatgat tgattcgctc gaccacttct actgctatat
     4681 catattcatt tggataaagg aatctcattt ctggcaacat aattgctgtg acatctctat
     4741 cttctctttt tcgttgaata gcaaaataaa tatgatctgt caatgtaata tagatatttg
     4801 aatctaatgg agaggagatc ctagctgtta tcagttcaat cacttcttcc gcaaaatcca
     4861 taaattcgat tggaatctgc gagactatct cactgaatcg tttgatcttt tctttagaat
     4921 ctaatacaaa taatttgtca gcatcctcaa catcaaatgg cttactcact ttagcagaaa
     4981 agccaatccc tttccccatc gcaatttttt ctattccatc gtcatcgatc aatacagcat
     5041 tatgattcag ctttttcatc acatacaccg ttaattcacc tcctctatat gataaaacaa
     5101 aacaacttgc agactccttc atacatattt ttgtctccaa acaattttta aagacaaaaa
     5161 acgacctaag aaagaaccct ttcttaggtc gtgcctaatt gaatagtagc accctacata
     5221 atttacaaaa atagtttaac acataataaa agcgttatca atatcttttt tactcgtttt
     5281 atgataatac ttgtatcgaa ttgatttgga ggtaattaca taaaaacaaa aaaaatatag
     5341 caactctcta taagaaagtt gctatattga taattatacc ggcggccggg gtcgaaccgg
     5401 cacgtcctca acggacactg gattttgagt ccagcgcgtc tgccaattcc gccacgccgg
     5461 caaatttaaa aataataact ggggtagctg gattcgaacc aacgcatgag ggagtcaaag
     5521 tcccttgcct taccgcttgg ctatacccca agaataaagg gcgaatgatg ggaatcgaac
     5581 ccacgaatgc cggagccaca atccggtgcg ttaaccactt cgccacattc gccatcataa
     5641 aagatgttat ttaatttctc aataaaaaac taagatgaag acatcttagt caataccggc
     5701 ggccggggtc gaaccggcac gccctcaacg ggcactggat tttgagtcca gcgcgtctgc
     5761 caattccgcc acgccggcaa gttgatttat tagtactaag gcggtaaccg gatttgaacc
     5821 ggtgatgaag gttttgcaga cc
//
LOCUS       DACCXO010000142         5795 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.149, whole genome shotgun sequence.
ACCESSION   DACCXO010000142 DACCXO010000000
VERSION     DACCXO010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.149"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            196..921
                     /locus_tag="IVY70_002602"
     CDS             196..921
                     /locus_tag="IVY70_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959698.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="IVY70_002603"
     CDS             911..1420
                     /locus_tag="IVY70_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959699.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="IVY70_002604"
     CDS             complement(1490..2938)
                     /locus_tag="IVY70_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7959700.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="IVY70_002605"
     CDS             complement(2938..3654)
                     /locus_tag="IVY70_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7959701.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="IVY70_002606"
     CDS             complement(3635..3985)
                     /locus_tag="IVY70_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP7959702.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="IVY70_002607"
     CDS             4129..4902
                     /locus_tag="IVY70_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7959703.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="IVY70_002608"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="IVY70_002608"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1689 a         1135 c         1001 g         1970 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tctttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaataagt ttttttaatt attatttgtc aaattaagcg agacaaaact
     5761 ttccatttac gtaactcaaa aaaacttcta atggt
//
LOCUS       DACCXO010000143         5764 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.150, whole genome shotgun sequence.
ACCESSION   DACCXO010000143 DACCXO010000000
VERSION     DACCXO010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.150"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(276..779)
                     /locus_tag="IVY70_002609"
     CDS             complement(276..779)
                     /locus_tag="IVY70_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP7959704.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1138..2163
                     /locus_tag="IVY70_002610"
     CDS             1138..2163
                     /locus_tag="IVY70_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7959705.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2486..3364
                     /locus_tag="IVY70_002611"
     CDS             2486..3364
                     /locus_tag="IVY70_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959706.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            3840..3956
                     /locus_tag="IVY70_002612"
     CDS             3840..3956
                     /locus_tag="IVY70_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP7959707.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            4884..5222
                     /locus_tag="IVY70_002613"
     CDS             4884..5222
                     /locus_tag="IVY70_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959708.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5316..5546
                     /locus_tag="IVY70_002614"
     CDS             5316..5546
                     /locus_tag="IVY70_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959709.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         1935 a          864 c         1061 g         1904 t
ORIGIN      
        1 acctgagtag ggtataaatt aagacggaat tcctttctag attgagatat tgactaggaa
       61 gaatgttaag attttaagcc tttaagctaa aaaaaaaccc tttttgttaa ataaggggaa
      121 tatagcctgt cagtttcaaa ctgcaagtcg tcctgctgca ataatatagt agattagaga
      181 tgaaaataga tttttttaaa ccaatgactg aataaaaaga ccgtcataga tacacattgg
      241 aatgtgtcta ttacggtctt tttttacgaa cgattttatt gaagacggac agttcgggtc
      301 aaaaataaaa aagctacgat cagaaataag aataaaatgt atcccaaaat aatggctaga
      361 cggtctaact tatttgtcga tagatgtttg tttagaagtt gataggccca taaacctatc
      421 agtcctcctg ttgtattagt tatcaaatca gtgatgtccg ctacgccaat accgaatagg
      481 aattgaatca attctgccaa gaagcttaca aggaaaataa tccctaattt tttagaaaaa
      541 gaaagtctct tgaaattgac attcaaaagc accccaaaag gaaaaaagac aatcaagtta
      601 taagtggtct cacgtaatac cgtcgtattt tcaaaagaaa aagtagaaaa agggacgaga
      661 ttgacattgc tgtaagaata agtgagaaat tgtggaagat taaatgatag cttgaataaa
      721 atcaaccata atagaaagaa taaataaatc cctaataaaa attttgatac ataccgcata
      781 gacatctcct ccatttaaaa agtataccat cgaaaaccgc tgaaaaatca taaaaaagta
      841 tgaagtttaa ggaccaacgt agttatcgta cgaaaattag aagctttttt ttgttgggaa
      901 tccggtttct ttaacaaagc taagatagcg agttgcttgg aaaagttgct atcttttttc
      961 tgtcaaaacg actcggaatg ttcgtttttt gttctaattt ctccaagttg tttacttttt
     1021 gatactaagt ttatgaaaaa tcaagaaata gttgttatac taggtaatgc tgacaaacgg
     1081 gattggcatt tcatcttggt tcagcttttt ttccaattac taggaaggag ggatagattg
     1141 ttcaagactt attttcgttt caataaaata caagataatc cttttcgtat catgaatctg
     1201 atgctactta tctttattgt attatggtta ggttacataa atcatgattt tttgatttca
     1261 agtttttcat cattagggat ctttactttt ttctattatc aaaacatacc gatgaaatat
     1321 ttgctgaaac gtatgtttct gatcggttgc ggactgttgg ttgctttcgt tttggggatc
     1381 ttatcgactt atgttttatg gcttgaacca tttgccgtag ctttggttgc tttttccagc
     1441 agattcgtgt tgcgattatt ccatatttct aaaccaggtg gcttgttttt tgcgatgctt
     1501 tctgccatgg gaacgagcat gcagctgcca atcgctcaat taccgatcgt ctcactttat
     1561 ttttttatgg gtgtcgtttt tgccctgatt gcggcagtga ttacaaaatt actggattca
     1621 cggcctgagc agacaattga aaaagcaact ttaaaagaaa gatttcacga agagccttta
     1681 gtgatcattg attctgtttt ctacagcgct gccttatttc tttccgttta tgtcagtcac
     1741 ggcttgaatc tgcataatcc ttattggttg accttgtcat gtgcatcgat tttgctagca
     1801 gaaaacttgg atgcgatgaa gcatcgacaa gtccaatatc tgattggatc gatgggtggg
     1861 ttatgtgtgt cggcattctt gtcatttgtg ccgtttacgc agttgcagac catttttctg
     1921 atcacttttc tatatggaat cgcacaattt ctagttgcta gaaattatgc agttgctaat
     1981 atttttctta atccgatggc gttgatgcta tctacactta ttcgtggagc atacttgatt
     2041 tctttgatcg aatatcgttt tttagggatc gtcataggaa gtttcatcgg tttaggtgtt
     2101 gcgtgggtaa tgacggttgg tttgcaacat tatttaacgg ttgttcgtca aaaattagaa
     2161 tagaaggaca aatatatgtg ccaaaagagg ttatgacaag atttttgtca caacctctaa
     2221 ttcaaggtgt tcaaaagcca gattctcagc attaagtcaa attcacacaa aaatgtggaa
     2281 aggaactttc aaaaaaattt tatatttatt ctatcaaaaa attttgttta tgatggtaac
     2341 tgaataaact ttcctgagat ggtcaatgct ttccttgaaa gtcttacaga tattctttct
     2401 tcatagaaag tttaagtttt atagttagtg tacaataaaa gtatgtttat accatatggt
     2461 gtccggataa tcagtcattt aaacaatgag gttcaaagat atgaaaaggg gaaatttgat
     2521 gaagaaaata agcaatgaaa tccaaattct ggcagaaact ttaactcttc ccaaagtcgt
     2581 tatagagcaa atgattgtac aagcaaaaaa catccttgat gaggtgtttg agcaatatag
     2641 tcatgaacta atggatccta ataaagcaaa ggcgactgca aagaaaatag acaaagtcta
     2701 tgaagaggaa ccttttgcag cattagctat ttatctatat gcagcttctc aaagttggaa
     2761 aaaaatttat caaccattga agattccaag aactatctat ttggcaacaa tgaatgcttt
     2821 tactagattt attcatgaac atttccaagt aacaggaagc tatcgctttg accgtggttt
     2881 ttggatttgg cattacacta gtggcttgat ctttcggatt ggtgaattgg agtacgaaat
     2941 gacatatttt ccgaaagggt acacggatct aagattagaa ggaaagcagt gcctttctct
     3001 tcatattcct tccgatgcag atttatctca gaataagata agagatagtt acgaaagtgc
     3061 cattcgtttt tttgaacttt attttcctga ttaccattac caagtgatgt acacagatac
     3121 ttggttacta tcacccaatc ttactaaatg gttgaaaaaa gagtcaaaaa tctgtctttt
     3181 tgcagccgat tatcgattat tatcagttga tgaacaggac gatagtggag tcccttggat
     3241 ctttggacga gtagatgccc aaatacatga ttatccagaa agtacgagtt tacagcgaca
     3301 agcaaaagag caattgcttg caggcgagca cattggctct ggcttaggta ttcttacttc
     3361 ataagtttag tacttcgaaa tagtttaaaa gagcgtgaga caaaagtaaa gaacactttt
     3421 gtctcacgct ctaaacacgt ataaacggcg gaagcagaag caactccttc ggaaataagc
     3481 tgaaattcac aaaaatttga aaagcaattt tcgtgaattt cttcttattt cttggagtta
     3541 aacgcttctg tcccagcctc ttttttagtg ttctatcgaa cacacagtac gttcaatagg
     3601 taaactagct ataacaatag atattaaagt taacggacaa gagggccttt tttaggtagc
     3661 taaaacgtaa taatgaaaat ttatttccag agataggagt caatcagcta gtctgatcga
     3721 ctgttttaac aagttgactt tcattttaac ttttgatacc atttaaaaca caaatcgtaa
     3781 ttgtttcgat ttgtgttttt tagcatgact acaatcaaag aagaaagtgg gtcgtccata
     3841 tggaaaaaac agatatctca agtgcatacc gccgcttgaa aagcccaaat atcaaaacac
     3901 gaaaacgtgc gctgaagatc atcaaagatg tgaaacggaa tagtgggaaa aggtaagaag
     3961 aaggaaactt aattaaaaaa atagccgaaa aacgtttttc ggctattttc aataatcggt
     4021 ttttgtgtaa ggtttccgaa agaactgctg ttgattttcc atttttaaca tatgataaat
     4081 tggtagaatc tttcttttca aattgatacc attccctaat tccagttata tagttaacta
     4141 cagaaccaca aagcacgtac gtttataaaa gtattatctg tagttatttt tcaattccct
     4201 ttcaacctct tattttgacc agtagtattg atcactttac cttttaacac ctttgttcgt
     4261 ttttgaaaca tttttagtca agaatggaaa cgtaactaca taatcaaagg ttttgcgaaa
     4321 tatttcatga aatatttcat gtagaagcga gcaatacggt tgcaaataac cattatcgtt
     4381 taataagcag ttacgaaagc tcccaatatc tctaacgttc agctcactcc atttcttttc
     4441 ttcaaaaaaa tcagtgattc gtatctcttt ctatttgtcg aaaaagttaa ctttaaaaag
     4501 taacgccgaa aaaatatgca aaaaaataaa aaagcagtta ctattgctag aataacatca
     4561 aggaataata agttttggtt ttcagataat agtcgataag ttgtgtattg gaattttaga
     4621 tacagtaata aagtattttt catgaaattt tgtgagaatc actattacaa gtatctttaa
     4681 taaaattaat ggttaaggag gaacttaaag tagaatgtaa atgaatagtt gtagtcagtc
     4741 gattgtgata ctagactcaa agttgcagga ataagttttt caatttgtga ttggaagata
     4801 aaaaatgaaa aagaagggct tttttttata ctttgttgat tgtagtgttt attttccctc
     4861 tgtttagcca agtaacattt tctatgactc aaccagtttc aagtgaggaa catgataacc
     4921 tttttcgtgc tacagaagag tttaagaaat atcaggaaaa tattgttccg gagactccat
     4981 tagctagtga gccaattact gatattgatg gaaaccaaat aggatggtat atacagtacg
     5041 aagttatggt ggtaaataat gactcaatag taaaaaatac agtaggctta gagctaacac
     5101 cgattgaaaa ggtgagttca ttgttaacgt atatttatac tgatgaaagt gacgagactg
     5161 agatcatttt aacggattac tcaaaaattg ctactgataa aaagtatata ttgtggatgt
     5221 agacactagt gaaatacttt atgatataga aattgatgaa aatttactag atgtagcaca
     5281 agaaatcgat catcaaaagc aggaagctat tcgatatgct ggtagaaaag ggaaaagaaa
     5341 atatttatta cgtgaatgta gcaaaagtta gagaagatga aaatgagtgg aaagaattca
     5401 agagtcgcta ctcaattaac tcgactccga catttacagt ttacagagaa ggatcgatag
     5461 aaaaaactgt tttttggact aaagaaagtg gaatgtcttt agctgaagta gaagagtttt
     5521 tggattatgt tagtatgcaa cagtaaggga gttcttatta tgaaaaattt tgacacatat
     5581 tatgttattg ttttttcgat atctttgctg ttaatattac taactttaat gcagtataaa
     5641 gagtatccag agtattttat tttaactttt gtatatttag taagtagcta cacacttcta
     5701 tataaattca gaagttcttc tagagtttct aaagtttgtt tcttaattag cttattgatt
     5761 ctat
//
LOCUS       DACCXO010000144         5740 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.151, whole genome shotgun sequence.
ACCESSION   DACCXO010000144 DACCXO010000000
VERSION     DACCXO010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.151"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..197)
                     /locus_tag="IVY70_002615"
     CDS             complement(<1..197)
                     /locus_tag="IVY70_002615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7959710.1"
                     /translation="MSISFSNNGRKSFSGKEVQIQYAALDIYWNIFSSTATEFYDEDP
                     QVVAFMMNTFFKKEVLDRLNSG"
     gene            complement(204..638)
                     /locus_tag="IVY70_002616"
     CDS             complement(204..638)
                     /locus_tag="IVY70_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959711.1"
                     /translation="MQTQEAIKTFILKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISFSQQTNYSASYLYTKMKKINAFLA"
     gene            786..1927
                     /locus_tag="IVY70_002617"
                     /pseudo
     CDS             786..1927
                     /locus_tag="IVY70_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(2053..3132)
                     /locus_tag="IVY70_002618"
     CDS             complement(2053..3132)
                     /locus_tag="IVY70_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7959712.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(3235..3675)
                     /locus_tag="IVY70_002619"
     CDS             complement(3235..3675)
                     /locus_tag="IVY70_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7959713.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3732..4142)
                     /locus_tag="IVY70_002620"
     CDS             complement(3732..4142)
                     /locus_tag="IVY70_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP7959714.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(4322..4756)
                     /locus_tag="IVY70_002621"
     CDS             complement(4322..4756)
                     /locus_tag="IVY70_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP7959715.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4856..5704)
                     /locus_tag="IVY70_002622"
     CDS             complement(4856..5704)
                     /locus_tag="IVY70_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7959716.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         2081 a         1074 c          954 g         1631 t
ORIGIN      
        1 ccgctattta gtcggtcaag tacttctttt ttaaaaaaag tattcatcat aaaagccact
       61 acttgcggat cttcatcata aaattctgta gcagtacttg aaaagatgtt ccaataaata
      121 tctaatgctg catattgaat ttgtacctct tttccagaaa aagactttct gccattatta
      181 gaaaaggaga tagacaaacc gtactatgct aaaaatgcat taatcttttt cattttcgta
      241 tacaaataag aggcactata gtttgtttgc tgactgaaac tgataatcga atttgtctga
      301 cagctgaaca aagaagtcaa gatctgataa ctgactgatt cgttgcagta tcttccaaat
      361 agacgataat agcagtaagc cggatcaatt ccttcaaaat tcaactttaa aaaaggtttt
      421 tgcttatcaa tatgcagttc tgttagattg agacgctcaa agtcttgttc taattctttg
      481 atgtatagta aaaaatttga ttcagacata tggaaatgat ctgctaaata ttgtgtagta
      541 ataaagaaac gatgttcaga taggaaaaga aaaatatcta attttgtatt gctttttttc
      601 ttcaagataa acgttttaat cgcttcttga gtttgcatga aagaggctcc tttttactta
      661 aattgttaaa acttgaaaat attactgttt ttttcataaa tttagacaaa aaaacagtaa
      721 acatataggt agaatctcta ctatcttatt tcttgcatac aaaaaaatca aggagtgaga
      781 gagttatgag atattcaacc atcttaatat caacatttgc agccttaagc ttatcgctta
      841 ctataaatca gacagtccac acagaggaga cagaaacagc caaatggaca gaaaccaagt
      901 gggccaacgt caatcgaatt acctttcagg atgaattttc atccttcaat tcaggagtcg
      961 caaaattaag aaaaggacaa acagcgacca tcaaagcaaa aattgactat tcgggagact
     1021 tagcacctat actacaatca gctgttacat ttgagaatgt tgatcccact ttgtcgattg
     1081 gctatgacga gaacactttg actatccaac acaagcaagc gatatttacc ttcaccgtta
     1141 cattaaatca ggacttgtta aaaccagcgc tttttactgt caaggtatca gatggactac
     1201 cgaacgatca tcatttaacg ccgtatagtc agcggcagac aattgagcaa gattcagcca
     1261 tcgactcagg aggagatctt gttgaagaac caacagataa accagaaaat gaaaataagc
     1321 cagaagtacc gcctacagaa aatccagacg gagaacagaa accggaaata gaacctggag
     1381 aagaacccga tacagaaact caaccagagc cagacaataa atcaaaacct gaaataaccc
     1441 ctggagaaaa accagatgta actccagaac cagatacgga ttctggaaac caaacagtac
     1501 cggaaacaaa tccagacaca gataatgaaa ctgaaaatcc agaaaaacca gaggtagatc
     1561 cagaagaaaa accagatgtg actccagaac cagatacgga cgcaagagac caaggaatac
     1621 cagaaaaaat aaacaaaaaa acaattcaag aggatggaaa aaaagagagt aaaaaatcaa
     1681 atctagcaat tctaaaaata aatgaagagc agctgaataa aaaatcacga atatttgata
     1741 gcgcccaatc agctgaaacc ttaaaaagta gcaaggatac aacctttgct gtcctgaaac
     1801 gaaaaataaa cagctaccaa aatccggaga gtcacaaaac aaagttatac tgtggagcgg
     1861 aatcattcta ttaagtatag caacaatgct ttctgcaaaa agatttaaac aaaacagatc
     1921 tctctaacat ttgaataatg tctgtgaacg catttttcaa ataaaaagaa atatccagaa
     1981 aatgacccca aaataccaga ttgaaatctg atatttgggg tcgttcatac tttatctttc
     2041 ttcatattta ttttaaggga tatctgtttc ttttggaaga tggttcggga tctttttcac
     2101 tttcaataca tctaaaaaga aggtaaagat cggaatgccg acaatcaagc cccaaacgcc
     2161 aaagaaccgt tcgctaacaa gtaagaccac aaatgtataa aaaatcggca gttctgtttt
     2221 gctggacatc aacttcggat tcagcacata agactcaatt agatggacaa tcaaaatcgt
     2281 aatcaggata taaacaacat ctcggatgcc tccttgggaa taagctatga aagatagagg
     2341 aatacaagaa atgataacac ctgctactgg gatcagactt aaaatgaaaa tcatgatcgc
     2401 taaactcagc agctgatgaa agccaaatac agccaaggcg attgtagtga gcaacgtatt
     2461 taaaagagcg acaacaaatt gagcttctaa taccaaacca aatgtattga caaacttatc
     2521 ggcaaaatag taaatatctt gaaagaacca agaaaattca ctttttaaaa acaatcttga
     2581 gaatacaatt gtttttttct tttcaatcat aaaaaagaaa cttaagatga atgacatagc
     2641 aaacgccact gccatttttc ctatatcctg taaatagcca agcgcaatcg aagcgccatt
     2701 ttgaagctgt tcaaaaaaat cgtttcgctc aatataagta tggatatatt gtaaaacagg
     2761 atctgcatct tttggctgat gctcgtaaaa attaataacg gactggatca tgtcaaatgt
     2821 ttgatgaata agcatcggga tatactttgt gaatgccaga tagataaggt aaacgatcag
     2881 tgtataaatc atgatagcta aaacacctgt agggattcgg acaaatcgct gaatgatttg
     2941 aacaagatga atcatcaaat aagtaaagat aaatgtgagc aaaatggttg taatcatact
     3001 tctaactaag aaaagaacaa aaacgacaaa taataaaaca gtgagacggc gtattttttc
     3061 attcgccaaa aaacgctgat aaacggtcaa atcgacaacc tccaaataac aaaaaataaa
     3121 cacgttattc attatatgat ggaatatgaa aataagaaag agcaaaaaga aaaaccgcta
     3181 aatgacgtta tcttatgagt tggttttcct cataaaatcg ttcatctagc agtttcagcc
     3241 ctttttctgg tcttgatgaa aattatctag catttttcgg gtcagatgtt gcagtgtatc
     3301 caattcttcc tgttcaagtg gaagataaga cttgatcttt tgtggaatac cttctgcttg
     3361 tttttgcaac gcagcacctt gagcagtcag cgtaacattt acgattcttt catcacccgt
     3421 ttcacgctga cgattaatat aatttttttc tgctaacttt tttagtaagg gagtcagcgt
     3481 accagaatcc agacacaaat catttcctag ctctttcact gaagtctttt tcttttccca
     3541 taaaactaac atcacaagat actgggtata ggtaagattc agcggcttga ggaggggagt
     3601 gtagtgtcta atgatttctt tagaggcggc atataaagga aaacatactt gattaactaa
     3661 atatagatct tccatttttc cacctccgct gttttactat agtgtatcat agcggattta
     3721 cacaaaaaag tctattcact aactgctttc agagtaactt ctatatttcc agctgtagct
     3781 tttgaatacg gacacacttt atgcgctgcc tctaataatt cttgcgtttc ttccagagaa
     3841 agaccttcga tagatccttc aatctctaca cctaagacaa agctcgtttc actttctgaa
     3901 taaagggaaa cttgtgcact gacagtagaa gccccagaga tatttttttg ctgtttgatc
     3961 aattctaatg cactgttaaa acatgcactg aaaccagctg caaacaattg ttcagggttg
     4021 gttgcatttt cgacgcgttt accaggagct gcaatattca attcgaatgt acgatcggga
     4081 ttatgcactt ctccagagcg tccgcctgtg ttgatcattt tcgtagaata catttttttc
     4141 ataaaattac cacctttaat tatattttat acaattgaat tttacacaat ataattgtgg
     4201 aaagcaaaga aatggattgg cataagataa ataagagaaa gcacatgaaa cagacgcgct
     4261 ttctcgaata gcaaacatat acaaaaaata tcagagacaa aaaataatct catcataaac
     4321 attacatata tttctcaaaa agttgttttc tttcttcaaa aattacttca cgatggacct
     4381 gatgaagcat ccataaataa ccaaaaggat ctctgaacat tgagttagtc acaccaaagt
     4441 ccatcatttc tgtcatttct tgaatctcta cacagccatt tcgtaatgct ttattatgtg
     4501 tgtcctcgat atcagagacg atgatattga accagatcgt atttggatgt tcagcagtag
     4561 gagcagtcaa accaagagac gtgttttcat ccagcatatg gaatctcgta ccatacaagg
     4621 taaaaatgac ttcattattg cctctagggt aatctgttgc ctcaatcagt tgtatttcat
     4681 caaagattgt ttgatacagt tctagtgctt tcaaactatc atttaataca aaatcgattt
     4741 ccgttccttt atgcataaag atctcctctc cttttcagtc gaacagttac tagattcttt
     4801 tattatacca gctaaagaaa acgcattcaa aaaaatagcg gatttactta ccaactcatt
     4861 cgattggatg ataagtaaat ccgcgtccca ttacttctgc tacgctgctg acaatgacaa
     4921 aggcacttgg atcgatttct tctatcaatg gtttcattcg ggaaaattca tgatcagtca
     4981 caacgatcaa taaaacctct tttgactttc gattataccc gccttttgcc tctagcaggg
     5041 taaggccacg tccaatctca gcacttaagc gaaggcggat gtcttccaaa taatgttcac
     5101 tcatgaccat aacagatttc tttcgattga tacctgtttc gatataagtc atgacaattg
     5161 aagtgattgc aatagataaa atagcataaa gaaactcttc tattccaaaa ataaacagat
     5221 taagagaaac aacgaatgcg tctgtaaaaa ataagccaat cgaagtattc aagtggaaat
     5281 attttttaaa aatgagggga gggatcgtcg taccgccgct agaagagttg tttttatata
     5341 aaatcgcaac tcctaaagca aaaatcgcac ttccaaaaat gacagacaac aaccgatctg
     5401 aagtaatcat tgtttctgga actattgcaa tgattaaagg aaaaaccaag cttccgaaaa
     5461 gtactttaaa aaagggtttt ttacctaaaa atagaagagc tagtaccaac ataacgatat
     5521 tcaacactaa aataactata gctcggtcaa agcctaaagc agcttccagt aatattccta
     5581 gaccgctgac acctccagca gcaatatgat gaggccctaa aaacatattg atagcgactc
     5641 ctaatatgaa gagggcaaaa ataatcttaa tgtatgtcca tataaattgt ctcgtttctt
     5701 tcatttattc acttccttaa ttcattaata atactcttat
//
LOCUS       DACCXO010000145         5677 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.152, whole genome shotgun sequence.
ACCESSION   DACCXO010000145 DACCXO010000000
VERSION     DACCXO010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.152"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            237..1628
                     /locus_tag="IVY70_002623"
     CDS             237..1628
                     /locus_tag="IVY70_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP7959717.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAEIDFSQEKIYR"
     gene            1687..2490
                     /locus_tag="IVY70_002624"
     CDS             1687..2490
                     /locus_tag="IVY70_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7959718.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGKRIVPEYNNAIRVFSKRRNPFNHMTVMFD
                     KKAILEVGNYQPLSGFEDYYLWARLLKAGYKGYNIQETLVYARTGKDMYARRGGMKYL
                     GSGLMGRYRIWKDGLGSVKDFIIVSCGHIFISLLPNQLRGKFYQTQLRK"
     gene            2523..3377
                     /locus_tag="IVY70_002625"
     CDS             2523..3377
                     /locus_tag="IVY70_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HAP7959719.1"
                     /translation="MKKIQQITLKMSQEIVDFCLKNQLLCYFCGGGAIGTVREQGFIP
                     WDDDLDFFMPREDYEKFYQLWVNSEKSKKYPIQKASKNYNDHNSFTTIRDADTTFIKT
                     YQRDLDIVHGITIDIFPLDVAPVSKFSRKIQKLWALVYALFCSQVVPEKHGGIMATGS
                     KILLSIFRSPRTRYKIWSFAEKQMTKYNNEPTGYVTELCVGPKYMGNVYPAKDFESAV
                     WVPFEDTEMPVPVGYDNYLSQVFGDYMQLPPEKDQVSHHEAVYIDPEHPYTMYKGKYY
                     LTKGAEKK"
     gene            3374..4075
                     /locus_tag="IVY70_002626"
     CDS             3374..4075
                     /locus_tag="IVY70_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAP7959720.1"
                     /translation="MITAIIIAGGVGKRMGQDIPKQFITIEGKPIIIYTLESFQHHPQ
                     VDRILVVCKSGWEETMWAYIKEFNISKVKWVITGGSKGQESINNGVQFLKDYSEDDDT
                     IIIHDGIRPLVDELVLSDVIVKCKEFGNGVTSLPYNEQIFIKKTEETTEQYVDRNTLR
                     RVSTPQAYQYGKLRAAYDRAVKEDIGMTDSSYTNTMMVDLGETLYFAAGSDKNIKLTT
                     TDDLELFKAYLKMKD"
     gene            4083..5150
                     /locus_tag="IVY70_002627"
     CDS             4083..5150
                     /locus_tag="IVY70_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP7959721.1"
                     /translation="MMTLIKQPMYQEDLAKVINHTPKIKQLHGKSVLMIGASGMIGSF
                     LIDTLMAANEQLGIKIKVYAMGRNRQKLEKRFASYLELDTFEIVEGDVTEPLPKEIQA
                     DYLIHGASNTHPKAYATDPIGTIMTNLAGTEQVLKHAVATQPVRVLFLSTVEIYGENR
                     GDIEKFTEDYCGYIDCNTLRAGYPEGKRVSESLCQAYIAKHDLDIVIPRLCRTFGPTM
                     LLSDTKASSQFILNAVEQKDIVLKSEGNQYFSYVYVGDAVSAILHLLLEGEKGEAYNV
                     SSENFDLRLKELAQQLADISGKQVIFELPDEIEQKGFSKASTAVLENKKIKETGWKPI
                     FPLNESLVHTVELVKGEKYNV"
     gene            5143..>5677
                     /locus_tag="IVY70_002628"
     CDS             5143..>5677
                     /locus_tag="IVY70_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7959722.1"
                     /translation="MCEISIIVPVYKVEKYLRKCVDSILAQTFTDFEVILVDDGSPDN
                     SGKICDEYAEKDNRVRVIHKENGGLSSARNAGIDVARGKYLGFVDSDDYIDEDMYEIL
                     YENLKIHDADISSVELIPFYGDRYKKANKEKKVIILNKKEAIKSVLEGTQFYAYAWNK
                     LYRKELFKDNRYLDGKTF"
BASE COUNT         2003 a          841 c         1104 g         1729 t
ORIGIN      
        1 atatgaatat tgtaacttgt gaataaggaa ttagcaggag aatactagtt cagcataaag
       61 gaagtgatgg gacttctgag acaactttct gttatgcaga aatacacata taaacagaca
      121 ataaacgctt ttccccgttt cttttaattt cctttacaat ttggttattc actaggtttt
      181 taaactttgc tatggtaaga tgatagaggc attattttat ggttaggagt gtatatatga
      241 ataaaaatgg ggagtggaat gcggcacgca gaatattcat tattcttatg gatgtacttg
      301 tattaaatgt atcgatctat cttagttttt tgctgaaatt ccagggagaa gtaccagtga
      361 gaaacttaga aacctttcag cattcagcaa tttttatttc catcatcttt attgcgttga
      421 atattttgtt aggagcttat gtattctata accgaatgat cagtgatatt gtcttcatta
      481 ctgttattgg acaagtacta atagcattgg ggatcatggt agttactttt gctggccgat
      541 ggtttgcatt tccgcgtggc gtcattttga tctcgtttat cctaggtacg atattactta
      601 gtgtttaccg gatagtcgtt tacaaactgt acttaagagt cagtcgtgat aagaaagtcc
      661 ttatcgtggg tttggagaaa gatgtcgcac cagctatcca aaattttcaa tctaagaaaa
      721 acaataaaca taaggttgaa gcagtggcaa tcgataatta ttacgaaaat acaaaaaaat
      781 tgatcgatga tatggatatc gtctatttgg cttctaaaat cccagaagat gaaaaaatca
      841 acatttatca attagtgact aaaaaagaga agaaattatt tttaaatacg acttttgaga
      901 atctgacaat gataaatccg aatattatga attttgaaga tgaaagcatc attgaagctt
      961 ctgggttcag aatccaacca gaatacgaac tattcaagcg tctctttgat atcatcgttt
     1021 cgcttatttt actgatcctc gcctcacctt ttatgctaat cacagcgatt ttggtaaaaa
     1081 caacttcacc tggaccaatc atctataaac aagtacgtat tacgaaaaat caaaaggaat
     1141 tttcaatcta taaattccgt tccatgagtg cgacagctga agcaaaatca ggaccagtct
     1201 tggctaaaag taacgatgca cgtgtgacac cagtaggtaa atttatccgt gctgtacgtt
     1261 ttgacgaatt acctcaaatc tttaatgtat tgaaaggcga tatgtcgatt gtcggtccaa
     1321 ggccagaaag accatttttc gttgatcagt tcaatgaaga aaatccttat tattacttaa
     1381 gacacaatgt acgtgcggga attaccggat atgcacaagt atacggaaaa tatgtatcag
     1441 attataacag taaactacgt tttgatttgt tgtatatcaa aaaatattca ttactattag
     1501 atttgaagat tttacttcaa acaatcaaaa tcttatttga taagatgtca tctcaaggac
     1561 tagatgaaga tgaaaatatt agttcattag cagaaatcga cttttcacaa gagaaaattt
     1621 atcgctagat tggaaaaatt ttgagcaaag aggtaggaga gaacattgga aaataaaaat
     1681 gtaagtatgc tgctttcttt atatgataaa gaaaagccag agtatttaaa ggaagcatta
     1741 actagtattt ttgagcaaac agtaatgcca aaagaaattg tattagttta tgacggccct
     1801 ataaatgaaa aacttcaagt agttgtcact tatttccaac aaaaagatcc tcacttgttt
     1861 acagttgtaa aattagaaaa aaatcaagga ttggggattg ctttagcaat tggattagaa
     1921 catacgaaaa atcgcttagt agccagaatg gatacagatg atataatgga accccaacga
     1981 ttagaaaaac aattagagat cttccaaaaa gatccagaaa tagcgattgt tgggagtaat
     2041 attgaggaat ttactggcac gcttagcaat gttatcggaa aaagaattgt acctgaatat
     2101 aacaatgcaa ttcgtgtatt ttctaaaaga agaaatccct ttaatcatat gaccgttatg
     2161 tttgataaaa aagctatttt ggaagtggga aattaccaac cattaagtgg gtttgaagat
     2221 tattatcttt gggcaagatt attaaaggca ggttataaag gatataatat tcaagaaaca
     2281 ttagtctatg cgcgaactgg caaagatatg tatgcaagaa gaggcggaat gaaatatctt
     2341 ggttctggtc ttatgggtcg ttatcgtatc tggaaagacg gtttagggtc agtaaaagat
     2401 tttataattg tttcttgcgg acatattttt attagcttac taccaaatca attaagagga
     2461 aaattttatc aaacgcaatt aagaaaatag gaggtttttc tttgtatagc actgatccaa
     2521 aaatgaaaaa aatacaacag attacgttga aaatgtctca agagattgtt gatttttgtc
     2581 taaaaaatca acttctttgt tatttctgcg gtggtggtgc catcggtact gtgagagaac
     2641 aagggtttat tccatgggat gatgatttag atttctttat gcctagagag gattatgaaa
     2701 aattttatca actatgggtt aattcagaaa aaagtaaaaa atatcccata cagaaggctt
     2761 ctaaaaatta taatgatcat aattctttta caactatccg cgatgcagat actacattta
     2821 ttaaaactta tcaaagagat ttagatatag tccatggaat tactattgat attttcccgt
     2881 tagatgttgc ccctgtttcg aaatttagcc gtaaaattca aaaactatgg gctttagtat
     2941 atgctttatt ttgttctcaa gtcgtccctg aaaaacatgg tggaataatg gctacaggta
     3001 gtaagatact cttatctatt ttccgctcac ctcgtacgag atataagata tggtcttttg
     3061 ctgaaaaaca aatgacaaaa tataataatg aaccaacggg ctatgtgact gaactttgtg
     3121 tcggacctaa atacatggga aatgtttatc ctgcaaaaga ctttgaatct gctgtgtggg
     3181 tgccattcga agatacagag atgccagtac cagttggtta tgataattat ttaagtcaag
     3241 tttttggtga ctatatgcag cttccgcctg aaaaagatca ggtttctcat catgaagcag
     3301 tttatattga tccagaacat ccatacacaa tgtacaaagg aaaatattat ttaactaaag
     3361 gagcagaaaa aaaatgatta cagctattat tattgctggt ggtgtgggta aacgaatggg
     3421 acaagatatc ccaaaacagt tcattacaat tgaaggaaaa cctattatta tctatacttt
     3481 agaatcattt caacaccatc ctcaagttga ccggatttta gtcgtttgca aatctggttg
     3541 ggaagaaacg atgtgggcgt atatcaaaga attcaatatt agcaaagtca aatgggtcat
     3601 tacaggtgga tcaaagggac aagagtcgat caataatggt gttcagtttt tgaaagacta
     3661 ttctgaagat gatgatacga tcatcatcca tgatggaatc cgtccactag tagacgaact
     3721 ggtattgtca gatgtaatcg ttaaatgtaa agaatttggt aacggtgtaa cctctctacc
     3781 ttacaacgaa caaattttta tcaagaaaac agaagaaacg acggaacagt atgtcgatcg
     3841 taacacattg aggcgtgtct caactccaca agcttatcaa tacggaaaac tacgggcagc
     3901 atatgaccgg gcagtgaaag aagacatcgg tatgactgat tcttcttata cgaataccat
     3961 gatggtagat ttgggagaaa ctttatattt tgctgctggt tctgataaaa atatcaaatt
     4021 gacaacaaca gatgatctag aattgttcaa agcatattta aaaatgaaag actaggtgaa
     4081 agatgatgac gttgattaaa caaccaatgt accaagaaga tttggcaaaa gtaatcaatc
     4141 atacaccaaa aataaaacag ctacatggaa aatccgttct gatgattggt gcttctggaa
     4201 tgatcggctc ttttttgatt gatacgttga tggctgctaa tgaacaacta ggcatcaaaa
     4261 tcaaagttta tgcaatgggc agaaaccgtc agaagcttga aaaaaggttt gccagttatc
     4321 ttgaattaga tactttcgaa attgttgaag gtgacgtcac agaaccctta ccaaaagaaa
     4381 ttcaagctga ctatttgata cacggtgcaa gtaacacaca tcctaaggct tacgcaactg
     4441 atccaatcgg tacgatcatg actaatcttg caggtacaga gcaagtattg aaacatgcag
     4501 tagcaaccca accggttaga gtattatttt tatcaacagt agaaatctat ggcgaaaatc
     4561 gaggcgatat agaaaaattc acagaagatt actgtggata catcgattgt aatacactga
     4621 gagcaggcta ccctgaagga aaaagagtca gtgaatcatt atgccaagcc tacatcgcaa
     4681 aacacgacct cgacatcgtg attccaaggc tttgccgtac gtttggtccg acgatgcttt
     4741 tatctgatac gaaagcatcc tcacaattta ttttaaatgc agtcgaacaa aaagatattg
     4801 ttttaaaaag tgaaggaaac caatattttt cgtatgtata tgttggagat gctgtttctg
     4861 ctattcttca tttactgctt gaaggagaaa aaggagaggc gtacaacgtt tcttcagaaa
     4921 actttgattt acgactaaaa gaattagccc aacagctagc tgatatcagc ggcaaacaag
     4981 taatttttga attgccggat gagattgaac aaaaaggttt ctcaaaagct agcacagcag
     5041 tacttgaaaa taaaaaaatt aaagagacag gttggaaacc aatttttcct ttgaacgaat
     5101 cattggttca tacagtagaa ttagttaaag gtgagaaata taatgtgtga gattagtatt
     5161 attgttcctg tgtataaagt tgaaaaatat ttaagaaaat gtgtagattc gattttagcc
     5221 caaacattta ctgattttga agttatatta gtagatgatg gatctcctga caatagcggg
     5281 aaaatctgtg atgagtatgc tgaaaaagat aatcgagtac gtgtgatcca taaagaaaac
     5341 ggcggattaa gcagtgctag aaatgcgggg attgatgttg cgagaggcaa atacttaggc
     5401 tttgttgaca gtgatgatta tattgatgaa gatatgtatg aaattttgta tgagaatttg
     5461 aaaattcatg atgccgacat tagctctgtt gaattgattc ctttttatgg tgatagatat
     5521 aagaaagcta ataaagaaaa gaaagtaatt atattaaata aaaaggaagc aataaaatct
     5581 gttttagaag gaacgcaatt ttatgcatac gcatggaata aactttatcg taaggaactt
     5641 tttaaagata atcgttactt ggatggaaaa acatttg
//
LOCUS       DACCXO010000146         5289 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.153, whole genome shotgun sequence.
ACCESSION   DACCXO010000146 DACCXO010000000
VERSION     DACCXO010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.153"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(142..804)
                     /locus_tag="IVY70_002629"
     CDS             complement(142..804)
                     /locus_tag="IVY70_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP7959723.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(814..1629)
                     /locus_tag="IVY70_002630"
     CDS             complement(814..1629)
                     /locus_tag="IVY70_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7959724.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1891..2334)
                     /locus_tag="IVY70_002631"
     CDS             complement(1891..2334)
                     /locus_tag="IVY70_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP7959725.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(2365..2478)
                     /locus_tag="IVY70_002632"
     CDS             complement(2365..2478)
                     /locus_tag="IVY70_002632"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7959726.1"
                     /translation="MEADNSNTLALLLEKLIRDYPSVQFHIHSGTSDDITE"
     gene            complement(2468..2806)
                     /locus_tag="IVY70_002633"
     CDS             complement(2468..2806)
                     /locus_tag="IVY70_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7959727.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2794..3171)
                     /locus_tag="IVY70_002634"
     CDS             complement(2794..3171)
                     /locus_tag="IVY70_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7959728.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            3395..4588
                     /locus_tag="IVY70_002635"
     CDS             3395..4588
                     /locus_tag="IVY70_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7959729.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4754..5176)
                     /locus_tag="IVY70_002636"
     CDS             complement(4754..5176)
                     /locus_tag="IVY70_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7959730.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         1608 a         1000 c          900 g         1781 t
ORIGIN      
        1 tacagtttct aaacctgttt cattcaaaag tatagcacgt tttatttcat ttgtttagcc
       61 atatgtttca aaaaatgaaa aagaatgaca gtttttacat aaaaactgtc attcttaatt
      121 gaaagtttca atttataaag tctacagatc catagttctc atcaatttac gattgttgaa
      181 gtaaatcaaa ccaaatacga tagaaggacc tagtagaata caccattggg gctttacgaa
      241 aaagaagaga aacaaacttg tcaatagtaa aaaatctagt aaactcacat accatttttt
      301 caacgtatag tagaacgcta tccgtagaac ttgatttggt ttcgacgctg gatttctcac
      361 ctgaaaatac cagtagttga tgctaaagga aactgataaa caagcaagta aaacaaacaa
      421 cggaatcaac cattttccat aagaaaaacg tataaaaaag aaacaatcaa aaatcattat
      481 cacactaact atccaagcaa atagccaata aaaaaatccc tttagccaaa attggcgaaa
      541 cgtctttatg aacaaacgta ctggtgaaaa acttttttct tttgctaatt cttctgtcat
      601 acctatcatc acgatgcaac ctggaccaat aaaaagtaaa gaaaaaataa aggaaaagac
      661 attccttcca tctatcgaca aaaggaacgc agctgctata tacaagatat tgacacacca
      721 aaaacaaagg ctacttgcca atgattgata caaccaataa aagaattcca tataaacatt
      781 tttgtcaaaa cgctgctctt tcataatcac ctcctattcc ttcattcctg atgaagcgat
      841 cccttctaca aaatattttt gcatcgctaa aaatataaaa gcaacaggaa gaacagaaac
      901 aactgaggca gccatgatta atgcgtactc tgcatcaaat agcccaataa acattttcaa
      961 ccctaactgg attgttttat tttccgtaga agacaaataa atgaatggcc ccatataatc
     1021 gttccatgta ttaacgaatg tgatgatcgt caaactggca atcgccggtt ttgtaagagg
     1081 taaaatgatc ttgcgataga ttccccactc tgataagcca tcgatccgcg cactctcaca
     1141 taaagaatct gggatactac tgtagtattg cttcaacaag aacacaccaa aagcattgaa
     1201 cgcttgtaat agcaccaacg accacaacgt atatgttaaa cccacttttc tcatcataat
     1261 gaactgcggg atcatataag actgccaagg gacagcaatc gtgccgatat atgctaaaaa
     1321 taaaatatcg cgccctttaa aatttagttt agaaaacccg taagcagcaa aactagaagt
     1381 aaacaactga atcaacgtga tcacaacgct taaaaaagct gtattcttaa aaaatgtcac
     1441 taaaggaata cgagaccaaa taaccagata attctctaaa tgccaagttt tagggatcca
     1501 ttgaatcgga atcgtgaaca cttcattatt tgctttgaat gaagcagaaa ccatccataa
     1561 aaaaggaatg atcgtcaata aagctaatac aatcagcaga atcataccca accattgaat
     1621 aaagggcatg ttttttgaac gaatttttgc tttttttctt tcatttcgaa cacctccaaa
     1681 gttataatct tcatttcttt gtaatgatta caatccagaa caacggttat tatagctgat
     1741 tcatttccgt tcttcttatg tttgtaagcg cttatttaac aagttatgta aatctcttta
     1801 ttttttttga tttaaaaatg aaaaaataca aaagcaagac tgtcatacgt ctcgcttttg
     1861 tattgaaatt tcttcgatta gagatactta ttactgttca aaagcttctt tgaaacggtg
     1921 aatcaattca ttgactacag gtgataaaat ccgattcttt ttccagacta gcatgcagtt
     1981 cgtttcaatc attgggtcaa gcggaacgaa tgtcactcgc tttgaaagat gacttacagc
     2041 accttctatg actaaagcag cacctacctg attctcaatt agcgcaaata cattgaaaat
     2101 caaattgtac gttcctgtga tcagcaactg ctcctttttt atgcctaacc agtcagcaaa
     2161 cattcgctgt atttctggac gctttgagat caaaagaggt acattcgcaa agtcttcttt
     2221 tgttatcacg tctttttttg ctaaaacaga attttctaaa atgagcaatc cccatttttc
     2281 ctttcttggt aacaaaatcg tttcgtattt ctctgtatct actggatcta gcaaaatcgc
     2341 taaatctaac aggctttttt ctagtcactc cgtgatatca tcacttgttc cgctgtggat
     2401 atgaaattga acgctgggat aatcacggat caacttttct agtaatagtg ccaatgtatt
     2461 cgaattatca gcttccacac agccgatcgt aaatcggccg ctgaataatt gtttttttct
     2521 gttctcgaat tcttgagtgg tttgattcgt taaatctaaa atttcttctg ctcggctttt
     2581 ttagaaaata acctgcttct gtcaagatca ttttgcggtt ttcgcgtaca aacagttctg
     2641 ttcctagttc ctcttctaac tccttcagtt gtcggcttaa ggttggttgt gtaagatgta
     2701 gaacttctgc ggcttgagag attgttcctt cttctgcaat tgtccaaaaa tattttagta
     2761 agcgtaactc catagcgatg ctccttgatt tttttacttg atacattcca ttttatttct
     2821 agataacaca cgatcatatt tcttgatttt tccttgtaaa cgttccaaac attcttgcat
     2881 ttcagcaatt tttttcgtca aaagttcact ttcttcaatc aaaagcttac gccgctcaaa
     2941 tacagttgct tctccttcgc tagataggcg aatatattca atcaaagact cgatcgataa
     3001 tccggcattt cgcatacaga tcgtaaagtc gacccatttt aggtcttctt ctgtataatc
     3061 acggattccg ccttttgtcc gattaactgg tggaatcaac ccgattcgtt cataatagcg
     3121 caatgtatcg gccgaaatgc ccttttcttc actgacttgt ttgatattca tctgatcact
     3181 ctttctctta atttcttgcc actttactta tgtattttat ttattctaca actttaagtg
     3241 tactccaagt caatactcat ttctattaaa actaagctgg attatctata tcggttgttt
     3301 gtgtattatt tcaaataaaa aagtcttgtt tttttattct tttttggata tacttttaac
     3361 aatttaggga aattttagga aaaggggtat aaaaatgaca acgtatcagc gaaagttatt
     3421 attatcgaca tcggcgggaa ttgctttaga gaatatggat attatgtttt tagctttttc
     3481 attatcttca atgattactt cttttcatat tagtggtacg caagcgggtt tcatttcaac
     3541 gatcactaat ttggggatgc tcattggagg tatttttttt ggcatcatag ctgataaata
     3601 tggacgtgtg aaagtatttt ctcaaacggt ccttctgttt tcgattgctt ctttattgat
     3661 gtattttgct tcaaacatct acttggttta tctctttcgc tttatcgcag gtgtaggagc
     3721 gggaggagaa tacggggcgt gtatgtcctt gatttccgaa agtttttcaa aaaaacaaat
     3781 tggacgagct tcttcggttg ctggtattgg tgcgcaggtc ggtgcggctt tggctgcgat
     3841 tttagctgct gtgatcattc caacgtttgg ttggaagatg ttgtatgtcg taggtgtact
     3901 gccggtgttg atggtcttgt tcattcgtcg cggcttgact gaaccaaagg aattcaaatc
     3961 tgtacaatct gccgaaaaga aaaccaaact tagcagactg tttgaatcaa aacatcttgc
     4021 ttggcaaaca tgtggcctta gtatcatggt aactgtccaa attgccggct attttgggtt
     4081 aatgaattgg ctgccttcaa ttatgcaaaa tcgattaggg ctttcggttt ctgggtcttc
     4141 attgtggatg gtcagtacta ttttaggcat gtctttgggg atgatgactt ttggcataat
     4201 catggataaa ttggggccta aactagcctt ttctttgttt ttaatttgtt catccttatc
     4261 tgtctatcta cttgtgttag cccatagcca gtggagtttg gtagcagccg ctgtgatagt
     4321 cggctacttt atcaatggca tgtatggtgg atatggcgca gtgattagta gtttgtatcc
     4381 aacagaaatc cgcgcgacgg cgaataattt cattatgaac cttggacgag ctgttggtgg
     4441 tttttcttct atcgtcatcg gtttcttgat ggatcattat agtttaactg ccgtaatctt
     4501 gtttttgagc ggtatttacc tagtcagtct gctggtcttg acaactctta ctggtgttac
     4561 agcattaagg gaaagcttgc agcattgata gttgagtctg taaatgacaa gaaagaggtg
     4621 tggcaaaagc ttgcatctgc ggtaataaga ccagccaaat caaaaacatg agacactgtt
     4681 tccgtttggc tggtcgtatt acttgatgca gggcagcttt ttcaactgct aatttccttc
     4741 tatctggcgg ctactattct ttgacgtccg tcatttcaaa gtactttact aataacttag
     4801 cataacgaaa aactgtggat acactcgtat attgagcgtc tgcataatct tccaaatttt
     4861 tctcaggatg ttgatacatg aattcaatca aatgataatt atatgatttt ctgtagaaat
     4921 gaagataaat aatgtagatt gggaagttct cctgcttttc catagaaaag cgccgattat
     4981 tctcattctt caaaatgacc ttatcaggat atttttggaa caattcttct aaatctgtta
     5041 gataattcaa gatagactta cttgatacct tcaattcgtt ttgtagttct tctattgaac
     5101 accaacgttc tgtttcaagc agtaaatgaa tcattttaat tttcttttga gaaacttttg
     5161 ttaaaaaagt ctgcatattt tctcctcctg tatctacttt actatactac atacaagaaa
     5221 aaaaacggat acaaatatca tattgtcaca ccgtcaatag tgcataaacc tgttattatt
     5281 tctcttttt
//
LOCUS       DACCXO010000147         5072 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.154, whole genome shotgun sequence.
ACCESSION   DACCXO010000147 DACCXO010000000
VERSION     DACCXO010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.154"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            25..324
                     /locus_tag="IVY70_002637"
     CDS             25..324
                     /locus_tag="IVY70_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7959731.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            589..1464
                     /locus_tag="IVY70_002638"
     CDS             589..1464
                     /locus_tag="IVY70_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959732.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            1461..1679
                     /locus_tag="IVY70_002639"
     CDS             1461..1679
                     /locus_tag="IVY70_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959733.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            1666..4293
                     /locus_tag="IVY70_002640"
     CDS             1666..4293
                     /locus_tag="IVY70_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959734.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            4308..4526
                     /locus_tag="IVY70_002641"
     CDS             4308..4526
                     /locus_tag="IVY70_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959735.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
BASE COUNT         2006 a          632 c         1003 g         1431 t
ORIGIN      
        1 attaattaaa aaggagaggt ttttgtggaa gcaataaaat taatgatttc agaagaacag
       61 gaggaaatgt ttctaaagca gttttatcaa attgctacaa aagctattga aaaggctcaa
      121 gaaaatgcgg ggatgacaag ggaatatctc aatcaaaaag aaatggcgga atacttaaat
      181 gtgagtgaaa actttttaaa aaatgctgtg agaaatgagg gattgcccgt agttgaatta
      241 ggggcaagaa aattttattc gaaaaaggca gttaatgagt ttatgtttgc tcgaatgaaa
      301 agaggggaaa atattggaag atgatgaagt agaaattata aaaaaatgct ttaaaaaaag
      361 aaaaaaagtg ttcccaaaag tttgcagact gggggaacac tcaataataa agtgctgaga
      421 aaactttatt tatagctatt atatcaaaaa agaaggtgaa aaggtgtttt cttctggaaa
      481 tgttgtagtt gacaaactta gagaaataaa ttttgacgga aatgtaatcc acacacatgg
      541 tacgatcaat taagaaaaaa aacgaaaaaa ggtgttgaga agccctattt gaatgcaata
      601 gttatcttag cagaaattac ctattggtat agacctaaaa aaatttttaa cgatgatggt
      661 cagctacttg gttatcaaaa aaagtttctt gaagatattt tgcaaaaatc atataagcaa
      721 ttaagcaaaa aaacaggatt atcagaacga gctataaaag atgcaattgt gtacttagag
      781 aaaaagggga ttatcaaaag ggtttttaga aatatttatt atgagaaaag agcagttaca
      841 ttatcaaatg ttatgtacat tgttttaaat gtacataaat tgatagaaat tacttttcct
      901 actttggatt gcgaaaaaaa ggagtatatg ccgaaaattc gtcagagggg tatcgaaaaa
      961 aagagatatg tctctttaaa aaaaggtaga ggtatgtaca aatttgtata tacgtataca
     1021 aagactactt cacagaacat atcaaagact actctcgtta tggaagaaga ggatattaat
     1081 ataaagagga tttacagtaa gattagtgtt ctttatgacg caaataacga agatggtcga
     1141 aaattatttc aagtgtatca ctatttgaga aacgctaatg tagatacaga agatatcaga
     1201 aaaatcattg aatatttatc tgaaaatact cagtatctta ttgctgagtg tattataaaa
     1261 caacatgaag catgcttttt tcaaagaaaa actgaagaag ggttatacga ttatgtaaca
     1321 tattttttga atggacttca gaaaagatat aaaagtcaat ttttagaaaa tgtagagcct
     1381 atagatcagt tcaaaaaagc tttcgaaatc aaagagttac ctgaaattac tatgcataat
     1441 tggttgaaag gagaagaatc atgattaaaa atcgcttaat agatcaaatg tggtgtcttg
     1501 atcaaaatac agatactctc gaattaatcg agagtatctg cttcaacaca gtagttctcg
     1561 atttaagagc tgaaaatgac aattgtgtaa ctcatatcat aattaatcaa aaaatggctc
     1621 agcagctctc tgagagccta agaaaatggg ctgaaggaga aaactatgaa agcaattaaa
     1681 gaaaaaatcg ttagtagaaa aataaactgg ttagcactca gtgtacttgt tttaatgatt
     1741 agcgttgttt ttacaagtaa tattgttgaa gctagcattt tcaattggtt tgatgacaca
     1801 aaaagtcaaa cagagttttt gaataattct ctttatagac cttacctaaa aaaatacaat
     1861 ggagatttcc ttagtcagtt cgggatttgg cttggatggg ctgtggtaaa aggcatgttt
     1921 actgtgactg attcgattca aaatatgatt ccagatgtgt tggatttgtt caatttcatt
     1981 gaaagtacag ggctaaataa tgtttatcaa tcagtcataa atacaattgt cgttggatta
     2041 atgatccttt cattgatgtt tgttggctac aagatgatta ctggaaaagg aacaatagat
     2101 ttaagatctg ttggaatgaa tatagtgatg tctgtagcgt taattctttt aatgccaaca
     2161 atgatttcta gcggtattca atttgccaaa atattttaca atgatgctac tacgattaca
     2221 aattcagatg acggagtcgc ttggtcattg ataaaacaag gagtaacaga tctggcatat
     2281 attaataaaa cagatcaata ctcaagcatt gataaaacgg aggatcgaaa taagttaaca
     2341 cgaaaaaatt ttcaacagac agatttaaca caagtgttga cggataaagt gattgataaa
     2401 ttggaaaaag aaaatccagc tgcggataat cttcgctatg aattagttga aaattctaat
     2461 aatgaatttg ttgccacaaa gttttctgat aacttcttat caacattttc tgatagcttg
     2521 aaatcaggtt attatcgata tcaagcaaat ctttggggga tttcgattgg gttgacagcg
     2581 ttggcaatcg cctatgtgtt cagcgcgttt gtgatcatta cagcaatatt agaacttgca
     2641 tttaaacgtg tgttaggtgt tcttgttttt gcgacagata ttgaaacagg acaacgttca
     2701 aaagttgttt tatccgatat tttacaatgt tacttaacag ttggatttca agggtttgga
     2761 ctatctatgt ttgctatgtt tatcaatttt ttaaatgctg gacaaggtat ctccacaaat
     2821 atttttataa aaacaattgc ttatatctgt gctgtgtttg tgctgataaa aggcagtgga
     2881 acagtcatga gatactttgg tgttgatatt ggactcaaag aaggctatgg gcaacttgct
     2941 tctgcttttg gtatgggcgc aatgctgttt agaaaaggat caactggatt taatcgtgct
     3001 aaaggaagcg gaaatggaaa tggatcagac agtggagaag gagaagatcg aaaaccagaa
     3061 aagaatttcg gcgaaactct atcaaaaaaa gctggcaaaa ctggaagagc tttgggatat
     3121 gcccatgaac gtggattatc aggtttagct tcagatggag ctacaatggc tagtgaaaga
     3181 gcaataaaac catttaagag tatgcgtgat atggctaatg ataccaagaa taagttcaaa
     3241 gaaggtttag atgatggtac agtgtccgct attaataaaa atagcaaacc tatgttagct
     3301 aaaaataaag aagatgaaac tggaaaatat gctgattcca tgccaacacg tctttcggat
     3361 agaaaagatg gtgctaaggt tgaaaatgcc gatagaatta tgtctagttc tgaacgtatg
     3421 cgtgaagcaa tgaaaaataa tgctgaatcg aataataatc ctgtaagtgc aattcagcaa
     3481 aaagttcaac aagatattga agaacgtaaa aatgctatgc acggtcaagc taagtctgct
     3541 gaagaattaa ttaatcagaa gcgacaagaa gctaaataca cgcctgaagc aatgaatcgt
     3601 gaggaaatgt taaggaaacg tgtagaaggg cgtacaggag ctaatacgga cgaaaaagaa
     3661 gcgttgacca aagaaagaat ccaagaagct aaaaactcaa ataccggatt agaaaaattg
     3721 gtcaaagaaa atctacaaaa ttctacaagt agtcaaggtg gccgatcagt agatgtccga
     3781 gaaaatattc aaggttcatt gaatggtcat gtcggacgga atgttgatgt aagagagaat
     3841 ttacaaagct cttcaaatgg tcaaggtact aagacaattg atgttcgaga aaatgtacaa
     3901 aattctacaa gtagtcaagg gtttaaatca atggatgtaa gagagaatgt acaaaattct
     3961 acaagtagtc aagggtttaa atcaatggat gtaagagaga atgtacaaag ttctacaagt
     4021 agtcaaggat tcaaaacagt agatgttcgg gagaacgttc aaaaagatag tggagaaatg
     4081 aaagaaaaaa cacaaaaaat caatatcgtt gaagacagaa agtcagcgaa aaaatttgat
     4141 tcaaatcatg aaacagtgat tattgatagt gaaatccgtg aaaatgataa gggatcaaaa
     4201 ccaagacgaa gatttaccta tgaagacaac gagttattca gagatacact taatgatcct
     4261 aatccgcttt tcgataatct attaaaaaaa taaaaggggg aattgtcatg aaattaattc
     4321 taaagattag tacgcatgat ttcttagaac ttgacgaatt atgtgaatca gtaaagaacg
     4381 caacaaatga aaaaatcacg gtcactaatt atattgaact gatgattcaa gaagaattaa
     4441 agatgaaaca tgagataaaa aacaacttga aatattgtta tctagaaaaa agtgaagaga
     4501 taatccatga attaaatggc ttttaaaaat tgtatttgac atgaatttac gacaaggaat
     4561 tagtggggat taagcgatta ttctcttcca ctttagagaa ataatgggtt aaaaagccag
     4621 aatacgtaga gcaatattga caacctataa aggagctatt gttgaaaatt gaagtaacaa
     4681 aaagataaag agaggtttac atctatcaga ttaaaagtga tagtacaccc atgaaacctg
     4741 caaaagagtt gaacctaatg tatatcggtt taactctttt tatttgcaaa ttaaaaaaat
     4801 atcaccgaag tgacgaatga aactatgacc aaattaagca agaaaatata tcttgcaaat
     4861 aaagtagtat aaaaagatat tgataaagcc aatcaaaact ttgaatttca aacgttagat
     4921 aatcaaacag atacaaaaaa tgaaatcagt tctacgcgta cgattattaa aaataataaa
     4981 ttttcactag aagaaaatgg aaataaaatc aaataattag gaagaaaaaa caaagcaatt
     5041 agaaaagcct gcaagcaaat ttgagtaatc ga
//
LOCUS       DACCXO010000148         5053 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.155, whole genome shotgun sequence.
ACCESSION   DACCXO010000148 DACCXO010000000
VERSION     DACCXO010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.155"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(81..1889)
                     /gene="pepF"
                     /locus_tag="IVY70_002642"
     CDS             complement(81..1889)
                     /gene="pepF"
                     /locus_tag="IVY70_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP7959736.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2065..3063)
                     /locus_tag="IVY70_002643"
     CDS             complement(2065..3063)
                     /locus_tag="IVY70_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP7959737.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3289..3942)
                     /locus_tag="IVY70_002644"
     CDS             complement(3289..3942)
                     /locus_tag="IVY70_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP7959738.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="IVY70_002645"
     CDS             complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="IVY70_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP7959739.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1498 a         1039 c          767 g         1749 t
ORIGIN      
        1 aaaaaaatac gtcgactccc ttacaagaga gccgacgtat ttttttcgtt tctattcgag
       61 aaaatcaaaa atagaaagcc ttataaacga tcgatcaatt cttctaattc atttagacgt
      121 tgttcaaaca tcgacattgc atcttcaata tatgcagcct gtgtcatatc aactcctgct
      181 tttttcatta cttccacagg ataatcgctg ttacctgctt ttaggtaagc caagtagttc
      241 tctagtgcct ctggttcctg gtttagtatt ttcttcgcca gtgctgaagc agcagaaaag
      301 ccagtcgagt attggaaaac atagtaatta taataaaaat gcggtatccg tgaccattcg
      361 aatttaattt ctgggtcttc ttcgactgct ggaccataat attttgcatt caatttacca
      421 taactatcac ttaggtattc actagtcaat ggcacacctt tttcatcttc cgtatgcatg
      481 aaatgctcga attcagcgaa ttgtgtctgg cggaaaactg ttcctttaaa cccatccaag
      541 taatggttga gtacataagc ccgtacacga gggtcttttt ctgtttccaa taaatactcc
      601 gttaggatat tttcattcgt tgtcgaagca atttctgcta aaaagatgga gtagtcgccg
      661 tacacataag gctggttcga acgagtgaaa taactatgaa cactatgtcc catttcgtgg
      721 acaagcgtaa atagctgatc cagcgtatca tgccaattca ataaaatata tggatttgtg
      781 tcatagcttc ccgaagaata agcaccgctt cgtttccctt tattttcgac aacatcgatc
      841 caacgttcag agaatgcttt ttctacgatg gccatgtatt cttcacccat tggtttcagt
      901 gcttctaaag ctttttcttt tgcttcttcg tacgtaaagg taattggagc ttcccctaag
      961 accggtgtat aaagatcata catgtgcagt ttttccactt ctaataaacg tttccgtaat
     1021 tccatgtatc gatgtaacaa aggcaaatgc ttgtttacca cgtctaccaa agtatcgtat
     1081 acactttcag gaatatgatt attgctcaaa gacgcttctc tggcagagct gtaattacgg
     1141 actttcgctt taaaattatg tccttttata tgtgtgccta aagtagaagc aaatgtattt
     1201 ctaaattgtt cgtaaacact gtacaacccc ttaaatgctg cttcgcgcac ccttcgatcc
     1261 gtgctttcta gcaactgacc atacacgcca tgagataatt ggactatttc accattttct
     1321 ccttcaatcg ttggaaaaac tagatctgca ttattcaaaa cagcaaatgt atcacttgaa
     1381 gcatcaaaga tttcacctgc tccagcaaga agagattcct gctcagcaga taagacatgg
     1441 gctcgattat ctactatttg ctggatataa tggcgataga cttccaattt tggttcttct
     1501 ttgaaatatt gccaaatctg gtcatctgac aattgcaata tttctggttc aaaccaggaa
     1561 acggcttcac taactttcga aaacagactg cttgctcttg cataaagcgc ttggtaatct
     1621 gtatttccag tgtcttgatc gtttttaagg tgcgcatata cataaatgac ttcagtttgg
     1681 cgataaaccc tcaatacgaa ttcaatcgca tttaaaaatt gagaagcgcc ttgatcaagt
     1741 gttcctttgt gtttttcaga ttgttttaac tcttctgata attccaaata tttctcatca
     1801 aactcttggt cgcttgaaaa gatcttggtc aagtcccaag ttaaattttc aggcaattct
     1861 tctcgttttg gcaactgctt tacttccatg aaatccactc cttttcttcc cttagctcta
     1921 tctatcaaaa tagatcatag tctaacatcc acatgtcaca gcatctaact tttctactca
     1981 ttcatgtgtc tgcttccatc aattttagca taactaaatc ttgttttaca tagaaaaccg
     2041 attcctagcc tatttttata cttactattt tttcaaagaa gacaagtgta tgcattcgtt
     2101 aaaaaactgc tgatagacca tctcttcatc aatcatagga aaaagccatt cccttttatg
     2161 ttcttttatt tggcaaagcc atgagttgaa atcaggaact ttctggtttt ccaaggcttc
     2221 ttcgtacaac attcgataaa gaaaaacctg ttcttgtaaa taaaagaaaa accgactttt
     2281 ttgatagatc cacgatgata gatgcaaaag atgtttatga cgcaagtagc attgttcttg
     2341 tatggatact gtattctttt gcttgtgaat aagttggcgt gtcagccatt tcttcccatc
     2401 ttcactcact ttttctattt ttttcatttg gttcatttta atttcgttac tagtgaatat
     2461 ttctttaagg tccctactag aaaaagtcca gcagctactg caaaaggaaa tatgccctga
     2521 tacagtctct gtaatatgat gatacaaata aagcttttgt ttcttgagat cagctttcca
     2581 gcaatgcact cctctttttc tattatagta gcagcaactt ttttctatca atgagaattg
     2641 cgattgtcca agaaaagaat taccaagtat ccaccatact tggtaactgt tattcaaata
     2701 attttgggtt ctttcaataa atctctggtg agacagtcta gaacattgga tttctattgc
     2761 tagattcgcc aacaaaatat ccggcctttg ccgcaacctc ggcaaatagg cttccaaaaa
     2821 tactggttct actgactgtc ccagccaatc catgaagcat tcctttaact gtaagtgctc
     2881 ttttgattct ctttcactaa agaccgaaca gttatctgat ttttgatggg caaaatgagc
     2941 acattttttc tttccctttt tcaaaatcac tctttttcga cagccacgac aataatattt
     3001 ctttcctttt tctgcatgtt tagcaaaaca gcacttatta tattcattca ttgcaagcag
     3061 catttttctc cctctgaact tatttacgtc taagaattta tttacacgaa aaaagagggc
     3121 gtgaaaaagc tattctgcat caagtaataa gaacagctaa acaaaaatag ttcccaatat
     3181 ttttcgtttg gttgggcttg ttaccgcaga tgcaagcttt tgaacacatt tatacgaaaa
     3241 aaagactgcg acacaatttt tgtcacagtc tcttactata atgctgattt atacaaaata
     3301 aaatttcgtt cgctcgatag cattacgcgc catcagacat tgtccatatt cattcaatgt
     3361 ttctggtgta cgatcgctct tttcagcaaa ctccaaaatt cgagcaatct gattctctac
     3421 atccgcttgg ttgagatttt ccatccagaa gtgaacgact aggtaatagc gctcttcgaa
     3481 taaatacaaa tcagtccaag cagactctag ttcgacatct gttgcaagtt ggatcacttg
     3541 ttcaaaatct tcaaaagcaa aagttgcttg tctttcctgt tcttctaggg cgcttccaga
     3601 taattcttcc tccggatcgc tgccaataat ctgctgacga agaaagtcac tcacatcttc
     3661 tgaatcaaca gtagaatctt ctgattgaga aatttcttca tccggaagat ttttactgat
     3721 aaacagttct aatccgtctc cttttggcaa aacttggaaa gtgacagctt cgcttcctcg
     3781 gaactcctct tcgatatcaa cttcttccaa aatactataa aagaaatttt cgatttcttt
     3841 gtgattgcca agtaaatcca aaaatgaaac gccccgttcc tctaaatctt catgaccaat
     3901 tagtacacgg atcgtatttt cattaatatg ttccatttcc attttgctac acctcacttt
     3961 gccacaatgt ttaatataga ttcattgtag cgaaatcagc ttaaatgtaa agaaaaaaaa
     4021 tctcgctgtt aagcgaattt aataaaacca gcggattttt caacctttca agctatctta
     4081 tttaactata acatttttgg cagacgaata ctatatagcc gacttttcag ctaattttcc
     4141 ctctctattt ttcgtgcaat catcatcttc tactaccttt ttatacatct aatcaagaat
     4201 actttttgta aaaacaaaaa aacttggaaa aactatcttt gtaatagtct tttccaagtt
     4261 tcgtattatc cttctataat ccagccatca attgtgcttg acgtaattct aattgtcgta
     4321 catcgcgcgg taggaaccga cgaatttcat cttcattaaa accaacttgc agacgtttat
     4381 catcaatcat gattggacgg cgaagcaatc caggattgtt ttgcacaagt tccaataatt
     4441 cttgtaacgg cagatcatct aaatccatgt tcagtttttg gaatactttg gaacgtgtcg
     4501 agatgatttc ttctgttccg tcttctgtca tttgtaaaat cgcttttaat tcagaactgt
     4561 tcaatggttc tgaaaaaata ttacgttcaa caaatggaat ctgatgttct tgcaaccacg
     4621 cacgagcctt tctgcaggaa gtacaacttg gggaagtata taatgttaac aacgtgcacc
     4681 actccttttc gtgataagaa tcactttttt gtaacagaag atcataataa tctcccatct
     4741 gtttctatta tacataatca acttttaaat agctaccttt ttttcataat ttttttaaaa
     4801 aaatgaaagt tatctaaggt taatatacag taaaaccagc attctcgctt tttttatcat
     4861 ttaaaaatta gagttttcaa atctaattca aattttctaa acatatgaaa cagcgtataa
     4921 aactgtacta ttattttatc tcaaaagcta aatgaaaccg atttcttaag attttttatg
     4981 ttttttattt ctttaatagt aatgttttct gtcattttaa ataatactgt tctttattat
     5041 aacgcttatt ttc
//
LOCUS       DACCXO010000149         4939 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.156, whole genome shotgun sequence.
ACCESSION   DACCXO010000149 DACCXO010000000
VERSION     DACCXO010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.156"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            326..1275
                     /locus_tag="IVY70_002646"
                     /pseudo
     CDS             326..1275
                     /locus_tag="IVY70_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300609.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(1320..1953)
                     /locus_tag="IVY70_002647"
                     /pseudo
     CDS             complement(1320..1953)
                     /locus_tag="IVY70_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(2213..2761)
                     /locus_tag="IVY70_002648"
     CDS             complement(2213..2761)
                     /locus_tag="IVY70_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959740.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(2762..3619)
                     /locus_tag="IVY70_002649"
     CDS             complement(2762..3619)
                     /locus_tag="IVY70_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7959741.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYVEVE"
     gene            complement(3905..4192)
                     /locus_tag="IVY70_002650"
     CDS             complement(3905..4192)
                     /locus_tag="IVY70_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7959742.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(4182..4511)
                     /locus_tag="IVY70_002651"
     CDS             complement(4182..4511)
                     /locus_tag="IVY70_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7959743.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(4588..4734)
                     /locus_tag="IVY70_002652"
                     /pseudo
     CDS             complement(4588..4734)
                     /locus_tag="IVY70_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            complement(4786..>4939)
                     /locus_tag="IVY70_002653"
     CDS             complement(4786..>4939)
                     /locus_tag="IVY70_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP7959744.1"
                     /translation="NKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKV
                     LLGIQA"
BASE COUNT         1496 a          939 c          720 g         1784 t
ORIGIN      
        1 ctaaaagttt tgtctttttt ctaagaatat cttccaacat atataactct tattctcttc
       61 tatcttctag cagatgttct gtctttttaa gacctagtat aaaaagaatg ggttcatttt
      121 ttctttcttg caggatgagg ttttttatat aaactttatt tgatataaag aaaaggcatt
      181 cataattgtg ctatactact aaaaaatgat aatacagtga attagattag cgctttatta
      241 aattaagagg acagcatatc gatattgctt cctttttttt gctatttatt taagggatag
      301 aagtttagaa aggactgttt ataagatgaa aaatgatata gtgacttatg caaacacaaa
      361 atgtgaagaa attggcatca gtaatttaga agagtgcttt tttgacacaa atgacctgtc
      421 gcaattttta agaacgggaa gaacaaatgc cagtaaactt gccaatgact tagttgcaaa
      481 gaacgttcta tttaaaatca atactcgtcc agtgttattt gccatcaaag agcgctttga
      541 agagcacttt ttgattccgg taaaagatgt atacgaaagt atatctgaat taaaaacaga
      601 cttggggccc aattctacag attccattct acaaaagatg attgggtatg atatgagctt
      661 atccgatgcg gtggaacaaa tgaaaactgc tgtcttctat cctggaagag ggcttcctat
      721 gatgatcact ggagatactg gagttggaaa aagttttttt gttcatactg cttatcagtt
      781 tatgaaaaaa gtgaacgtta ttcatgagaa tgctcctttt aaagtactta attgtgcgca
      841 ataccataat aatcctgaat tattgtcggg gatactattt ggctatgtta aaggtgcttt
      901 taccggcgca gatgaagatc gaaatggatt gttgcaagat gcagacggtg gagtactgtt
      961 tttagacgaa gtacacagat tgcctcctga agggcaagag aaactatttg tttatatgga
     1021 tggcggaact tattctagag tcggtgaaac tatccatcac gcctccaatg tacgtttagt
     1081 ttttgcgact acagaaggca aagaggattt tctggaaacc tttatgcgaa gaatccctat
     1141 acatatccat atcccaaata ttgaggaacg aggtatttta gaaaaaaaca aattatcgaa
     1201 aatactctct ttagcgaaag caaaaacttt agcaaggcta ttgatgtttc tcctaaagtc
     1261 actagtgttg cataaatata gtcagaatat tctgaattat tattttcttg aaaaaagtaa
     1321 aaaaaatcgt atcccaaact ttcatctaca tttccagaaa gatcttcatg ttgataatca
     1381 attcctgaat caatgaccgc taccttcaca tccttagaac ccttggtttc ttcccaagcg
     1441 gatggcatgc taatctctct taaattagct tgtttactcc aattaggatc gttatcgatt
     1501 tcctaaagaa tcagttgtat ttggctcctc aacatcattc aacggctcta gttcaaccac
     1561 atagtttggc tctgcataat tgaccatatc agaagcatta aatgttctta ctgcctcttc
     1621 cacactaatt tcttcgttca atttcactaa taatacttga tctcgcttat tataatcgct
     1681 ttcatttgtc ctaagagtca aatctttttc caccataact tttacaccca ataaattttg
     1741 cggtttattt ttctctgttg aaattctttg tgtagccttt aaactgacaa ttagctcatt
     1801 gggtacatac tctatttgag agtcatctaa ttggctcata atcctttctt tttggttagt
     1861 atctgcagtt acttgtttag tatgagccat gaacccaaac atactcaccc caacaaccaa
     1921 tcctaaatat attttctttt taaacttttt catctacatc tcctcctaat agttttattt
     1981 tagagttagc tctattactt atttactaac tcaacaacag attatcatac acttattaat
     2041 gaaatcacaa tatctcatta aaacacaccc taaaatcata accgttccta tgctcatttt
     2101 ttggattttt taatcacgta accagaaaaa taaatcccct ctatttcacg caaatcaaag
     2161 aagaactaag taactaccgt ttaaaggtag ttacttagtt cttctttgat tattatctaa
     2221 ctttattatt cattccccac cctttgcgca gtgagtcttc ggtatcttgt tttatctgac
     2281 tggtagttct tattatttct ccacgatcat ctaattctgg aacaattgga tatttttctc
     2341 tgaacaagtc tagttctttc accacgtcat taaacaaatc ttgtttatct tcttgcgtaa
     2401 gattctgttt aatatactca tctaaagctt ttctaagcaa ttcatctctt gtcatattag
     2461 tctcatactt ttctactaaa actctggaag tcgcatttaa caaagtcgac agttttaaag
     2521 gatgcttggt ggaataaacc ttatcaaact ttctgattgg tgcgccaact ttttttgttc
     2581 ttcctctact ctgtctttca acattggagg aagtttttat ttcttgaact tggtgcactt
     2641 ctaattgctt attttcagat ggtgtaattg cttgctgctt tttctttgct tctgcttctt
     2701 tttttatttt ggcgagtcct ttgttgttcc caaaaatatc aagtcctttg atgtcagcca
     2761 attactccac ctccacatac caattctcgt gttcttccaa ttctttcaaa atactgataa
     2821 ataattcgtg gcttttgcga tcccattgtt ttaagagacc tttctttgtc ttctccatcg
     2881 taatacctga tccgtcgtat cgttttaagc gcgcattttt gagtacaaca tttttaatca
     2941 aatgttcccc ataaatctct tgggcttgtt tatacatttc ctgatctact gcgtcaccgc
     3001 tttccaacat gaaaggaata acaccagcaa catataaatt tgtatcaaat tctttcacaa
     3061 aaaagtccat atactcaaga tattgcatcg catttctcat cgattttact tgggtctgta
     3121 agacgacgat tgcccaatcc gcaatgtaca tcgcggattt actatataca gaaatagaag
     3181 gtgggacatc taaatagatg acgtcaaact catcaaataa tggttctagc ttttctttca
     3241 tatatttgaa ttgggataat gaatcattat attggtcttt gatccaatca tcatacattt
     3301 caaaggacat gtctgctgga agtatataga ggttattgat tactttatag acagcctctt
     3361 taggtggaag attgtttatc aacacgtcag tcacagtatt ttcgtaaaca atcggttcac
     3421 cttctccgcc tccttcttcg ttagtgaagt tcatatgcgt taagttcatc acatcagaag
     3481 tatttccttg catatccaag tcaattatca aggttctttt tccttcatta actgctgatt
     3541 caaaccctaa catttgcgcg cacgtacttt tacctacgcc acctttaaaa ttaccgatta
     3601 catatttctt cgctaccacg ttcctacctc ttttctttta gcaatattca tttgatacta
     3661 ttataacata tttattttta gttactacca aaaaaaagat atatatgtac taccttataa
     3721 ttaataagta aaaggtagta cataaataag gaaaagctga aatatattga tttatagctt
     3781 tcctcaaagt aatgacgtta tttaatttat cgagaagcag agaatactcg cgactttagt
     3841 catgtagacg aatcagattt tcgataaaaa agaagccgtc cttcttaaga acgacaacaa
     3901 atcactagat taattttttc aactgttcat aaaagttttc atgcgttcca cataacaata
     3961 ctggaataac cttgttttct ataatctcgt aagctactct atatgttgtg cccgcatatt
     4021 tgaacccact agaccaaata cctgataaat ccccagtttt ttgttcaccc ttatatggat
     4081 caatcgcaat ttcatcataa attaaattca aaaatttttc tttcaatggc ttctctttta
     4141 gcttttttaa atacttaatg acatgttttg aaggaacgat ttcataagcc aatttctgtt
     4201 gccaactcct ctttagtcat tactttagaa ttagtaagtg tatcttcagc aatatctcta
     4261 aaggaagaat gcattttgtt ttttctattc ttaaattcta caagaatctc atctttatcg
     4321 tagccttctg caattatatc agacaaaata tcttcgctaa aatcataaaa ttctttttgt
     4381 ggttcaacaa attcataaag aataccattc tctaccaact ttgcttcaac ttcgactcca
     4441 ttaggaacat taaaatcttt aggtactgtc aacatgattg aattgccttg ttttcttgtt
     4501 tttgtaatca tttaaatccc tcatttctaa cagtatgtaa ttacttagta aatacattat
     4561 accacgagat aatgtattta caagtaatta ctgtgactcg taatgcgtcc ttgctgaata
     4621 aatttcaacc acgtgttcat cttcatctac cttataaaca acccggtgtt gtctattcaa
     4681 tcttctaaaa tacctacctt catgtgtagg ttttaacttc tcaaatgatt gggttctgtt
     4741 gcaaagtttt aaataaagaa taaaatccct tacggtatct atgatttaag cttggattcc
     4801 caataatacc ttgatttcag tacagaccga aaacccgaag agagtgcctt cttttcgggt
     4861 tttcttatat aatcctcgaa tggcttccat gcctttaatc gtggtagagg cagtgcgtaa
     4921 acttcgatag aatttattg
//
LOCUS       DACCXO010000150         4901 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.157, whole genome shotgun sequence.
ACCESSION   DACCXO010000150 DACCXO010000000
VERSION     DACCXO010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.157"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            10..696
                     /locus_tag="IVY70_002654"
     CDS             10..696
                     /locus_tag="IVY70_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP7959745.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            698..1642
                     /locus_tag="IVY70_002655"
     CDS             698..1642
                     /locus_tag="IVY70_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP7959746.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1841..2485
                     /gene="pcp"
                     /locus_tag="IVY70_002656"
     CDS             1841..2485
                     /gene="pcp"
                     /locus_tag="IVY70_002656"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP7959747.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2564..3841
                     /locus_tag="IVY70_002657"
     CDS             2564..3841
                     /locus_tag="IVY70_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959748.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(4025..4774)
                     /locus_tag="IVY70_002658"
     CDS             complement(4025..4774)
                     /locus_tag="IVY70_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP7959749.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         1578 a          866 c         1053 g         1404 t
ORIGIN      
        1 ggaaaatata tggaaaatta tcttagcctt ttaggcgtct tgattgttat tctcgggttt
       61 gcatttaaat tagactcgat attgatcgta atggttgctt tagttgttac agctttgact
      121 ggcggtcttg gcattcaagg aatgttagaa cttttaggta caagttttgt aaacaatcgc
      181 gggatggcga tttttatcat cattatgttg gcaacaggaa cgcttgaaag aaatggtctg
      241 aaagaatcag cggcttcctt gatcaagcga ttcaagaaag tatctgctgg tatgattgta
      301 gatatttatg ggatctttcg aatgatcttt gcggctttta acgtgagttt tggtggagtt
      361 gctggatttg ttcgtccaat cattttaccg atggcattgg ggaccatcga atcaaaaggg
      421 ttagaagtcc atcctaagca tgaagaagaa ctaaaaggta tggcttcagc tatggaaaat
      481 gtctgctggt tctttggcca agtactattc atcggaggag caggggcgtt gctagtccaa
      541 tcaacactga aagatctagg atatgacgta acattgggac agcttgcttt agtggaagtt
      601 ccagtggcta tcacggcttt agttgtggca agtgtctact tctatcttaa agaaaagaaa
      661 ttagcgaaga aatattatcc aggaaagggg caataagatg gactttttta caaatcctga
      721 tattttatta ggcgataaat tactggagat cctttatatc gtgatgggac tcgtattgat
      781 ttacactgga gtaagaaatt taacagataa aaccaatcct catcgttatg ggtcagcctt
      841 tttctggacg gtcttagggg tagttattgc aggaggaaga tggcttccgg caatcgtgaa
      901 cggtggcttg atctttgcga tgaccatacc agcaattgct aagaaagtca gcaaaggaga
      961 aagccggctt ccatcaaaag aatatatgga aaaaatgtca gacaaactag gaatgaaaat
     1021 ttttattcca gctttgagta tcggtgtctt tgcgatcttg tttgctctat ttaccaattt
     1081 aggtgcttta gtcggcgtgg gcgtcggtgt ttttgcagcc atgctaatct tgatgttctt
     1141 ttctcgagat aatcgaccaa gtacattttt agatgatgca gcagatatgc tgggcacagt
     1201 agggccgtta agcatgttgc caatgctttt agcttctcta ggtgcggtat ttacaagcgc
     1261 tggagttggg acagtgattt ccaatgcagt aggtacgatc gttccagaag gaaatgtgaa
     1321 catcggcatc atcatttacg ctcttggcat ggtgatattt acggtaatca tggggaatgc
     1381 atttgcggcc atcaccgtga tgacggttgg gatcggtgca ccattcgtat ttagtcatgg
     1441 agcgaatcca gcgttaatag gaatggttgc attgacttgc ggtttttgtg ggaccctgct
     1501 gacaccgatg gctgctaact tcaatatcgt gcctgtagcg atgctggaga tgaaagacaa
     1561 atacggagtg atcaaaaacc agttgtttat tgcgttattt atgctagttt tccaaattgc
     1621 ttatatgatt ctatttaaat aatagaagag aggggttgtg acagaataat tgtcgcaacc
     1681 tcttctaaca ttgtagtgga acactatttt tttataagaa tatattaagt ttgatctgtt
     1741 atgttattta aactattgtt tcctttattt acgctgttca aggaaaatgg tatgataaaa
     1801 gataaccata tgttaaatca atcggacgga ggaacaacag atgaaagtac tagtaacagg
     1861 ttttgatcca tttggtgggg acaaagtgaa tccagcctat gaagcagtaa aaaaaatgcc
     1921 agatgaaatc agcggtgctg aaatcatcaa agtggaagta ccgaccgtat tcgaaaaaag
     1981 cagtcaagta gtcaaagaag ccatccaaca gcaccaacca gatgtcgtga tctgtgtcgg
     2041 acaagcaggt gggcggtcag cagtctcgtt cgaacgtgtc gcaatcaatc tagcagaagc
     2101 aagaatccct gataatgaag gaaatcagcc attcgatacg gctttagaag aaaacggtcc
     2161 agccgcttac tttacctcat tgccaatcaa agcaatgaca aaaaatgtac aagatcatgg
     2221 tctaccagcg tacatctctt atacagcagg aacatttgtc tgcaatgata tcatgtatcg
     2281 tttgcttcat ttgatcgaaa cacaattccc ggcaattcga ggtgggttca tccacgtgcc
     2341 attctcaccc gatcaagtga tcgatcgtcc agtgggtacg ccttctatgt ctttagaaga
     2401 cattgcttct tctttgactt atgcagttga agcagcagta gaaaataaag aagatatttc
     2461 cggaaatgcc ggaacaactc actaatttag caagaaacac tggctggttg ctaacgttat
     2521 gttagtagcc agctttttgt aattagaaag gaaagaaaca gccgtggaag aatggcaatc
     2581 cgtctttgaa gaatggtttc caaaagaaat cagcaaaagc tatcccatca aaatttctaa
     2641 acaatacaca agcagtcaac gttgggagat ctatgcaaaa ttaacgaaaa aacagcggga
     2701 actagtcgat aagcaccgcc gatatctaat cagctctcgt tttatggaag aacattattt
     2761 agctgcaaca gattgggtgt tttctgactt caagatcaat ccgttttttc gaacaaaacg
     2821 aagccagcaa aaattgtatt gtgagtgtgg tagagaatta aaagtgcaat acatcgttaa
     2881 atcgccaaaa accggaaaga tcctaaaact aggcatcaac cactttgccg atcacctgca
     2941 tgtctctccg acagtggcag cttcgatcca tcagggaatg acaaaagttg atctggcctt
     3001 agacgaatta ttatggctta agcagaagaa tatcgatttt cctgaaggac tatggcaaaa
     3061 atactgtttt gtcttgtacc aaaatcgtcg gatgaaacag ccatatttac cggatataaa
     3121 attagctcag cggctcgcag aatttcgcca agtagaaatg ccgatttaca tagcggatta
     3181 tcaagcattg gaaaatgaaa tcaagaaaat aagtgaacac atcaatgggc agtcaaaaaa
     3241 gcgacagata aaaaaagaac tatttgacga ctttgcagaa gaattagtaa aggatgtaga
     3301 agaattcttg atcaactatc gcgcattttt acgtaaagat tggcaatcca tcgtatatga
     3361 agaagtgcca gtacatccta atgcttattt tgaaacattt atttccgtgt tgagaaagac
     3421 aaaacgtcaa cgaacaccag aagtcactgc tcagatggaa tattttgcga aaaaccaacg
     3481 attcatccaa ccaaaaatct atctatttat ttggaagcaa tattgtcgct atggttttac
     3541 agaagggttc ttcgacagca ttcctcggat agtcagaaat ggttttttaa aagtattgcg
     3601 taaagaacga gaagctatac aatctgctga taagaaagac cgtacagtct caaaagaaaa
     3661 atggcaacta gtagtgaaag atatccaatc tggaaatgtg caagagacaa tcgacaaatg
     3721 gaaaggaaaa cattatcgtt ttactgaagc tcagaaacaa gcgttggaat attatcagaa
     3781 gttagaagaa tcactgcgtt tcaatgatga ggcaagaaag tatctgaagg aattactata
     3841 aaaaatagag gctgggacag aagcgtttaa ctccaagaaa taagaagaaa ttcacgaaaa
     3901 ttgcttttca aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgattccgc
     3961 cgtttatacg tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcagt
     4021 ttttttatgc gtgttgttgt gcgttgtagt attcttcttc cattttttgc atacgttcac
     4081 gttctttttg gttataccaa ggtgaaacgg taaatgcaag gacatcattc agcaagtaaa
     4141 cagagctgtt cacgaacatc gccaaacttg cagatccatg cgtaaatgaa atataccaaa
     4201 ggaccatttg agcaagccct gaagctaacc accagtaata ttggttgttg taacgcaaga
     4261 aactgatgat accagcagtc aagctaatag aaaaagcaat ggcatctacc cacggccgag
     4321 gatcatctgt ataacgacca atcaggaatc ctgaaacaag atagacaagg aaagtactga
     4381 tgattgcgat aaaccagctt tttccagtga attttcggat ttttgaagcc atattatgat
     4441 tccattcttt acttaacaat acggggatat ccaatgtgat catataagca atctgttcac
     4501 cgatacttaa atagttcttt gctgaaaacc caacgataat aaaacaaatc gcagacaaaa
     4561 tacctaaaat accgttgact gattttgccg cattgatgga taaaatacac aatacaccaa
     4621 gcgtggtacc gataaatgta ataacggaca gccaagtgat gggactattg acaagagtca
     4681 tgacctggca gccaagactg aaaaagaaaa gataataatt ttgttgcggc cagcctttta
     4741 gctgatgagc taaaaactga aaatatgact tcatgtatga ggactcctta agacactaaa
     4801 tgtagtgtgt tctgtattta ctaacacaat ctattgtatg cttttcttga aaaaaaacta
     4861 gtctttcaat tataaataat ttgtgttatg ataattttct g
//
LOCUS       DACCXO010000151         4687 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.158, whole genome shotgun sequence.
ACCESSION   DACCXO010000151 DACCXO010000000
VERSION     DACCXO010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.158"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(118..1080)
                     /locus_tag="IVY70_002659"
     CDS             complement(118..1080)
                     /locus_tag="IVY70_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7959750.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1082..2521)
                     /locus_tag="IVY70_002660"
     CDS             complement(1082..2521)
                     /locus_tag="IVY70_002660"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP7959751.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2706..4652
                     /locus_tag="IVY70_002661"
     CDS             2706..4652
                     /locus_tag="IVY70_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP7959752.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1479 a          803 c          777 g         1628 t
ORIGIN      
        1 gtctaacttt tgggggtcac tacaagttga caactccttt ttgtttacaa atcaattgga
       61 tatctacaaa ttgtattttc taacgaattt tatagacatt aaaaaattaa tttaacgtta
      121 aatacttcct ccagaaagta ttgagattgg taaaatataa tccttatttg tcttttcgcc
      181 tttaattttt ttcagaagaa tatcaacaat aagacttgca atttcatcaa ttggttgtct
      241 tattgttgtc aattgtggga aaaatttctc aatgaaagag gtaccatcgt atccaataat
      301 ttttaaatct tctgggatat taagtttaag ttgatgagca attttcatag tgagaatggc
      361 tgttaaatca tcagaaacaa aaataccatc tggtttgtga tttataatag ttgactttat
      421 ttccatttca cgacggatag tagacagatt attaggaatt ttgaaaattt ttccatttgg
      481 attttgaaaa gaaaaaccta gtgcacgtaa ttctgtggga gaatccgtgt tatcatttcc
      541 tgtaatcata atggtatttt gacaaccatt tttttgtaaa gtcttagctg caagttttcc
      601 cccttcaaag ttatctgaag aaacaatgga aacattagga gctaaatttc tatcaaaagc
      661 aacaataggt gctctcacac gctcataatc agcaattcca agattatggc ttgatgagat
      721 tatgccatca acttggttgg cttcaagcat ttcaatgtat tctctctctt tagcaggatc
      781 attttgacta ttgcaaataa tcgttttata tccatgttta aataactcta tttcgagata
      841 ctcaatgagt tcagagtaaa agatattact aatattagga aaaataagac caataagctg
      901 aggtgacttt ccttgaagtc cacgtgctaa attatttggt ttataaccaa gagttttcat
      961 agcttcatga actttttgtt ttgttttttc tgataaatat cccttattat taatgacgcg
     1021 tgaaacggtt gttgggctaa ctccagctaa ttcagcgaca tcagttaatt ttgctaccat
     1081 tttaatattt taattcaaag tattttccag taggttcgcc tgatttgatt tgaataccat
     1141 tttggtgatt ttcagggaat acacgactag taaacacttt ttctccttta ttaataaaaa
     1201 tctcaatgat tgagttatct acaaaaatgt tagcagttgt ttctttagta tcaattgagc
     1261 attcacgaat cgtaccaaag tcagtggcat attgaatgcc tgctttgctg cgatctaaaa
     1321 taatttttcc ttctttggta tctacaatca agctaagtcc atttccttta ctatcagaga
     1381 aaagaagtaa ctccgatttc tgatttggag gaaaagttag ttcaagctca taagtattat
     1441 ttgtttctgc ttttgctgta aattgttcag aactagcacg taatgatgta atagcttcta
     1501 ctggatattg gtaaagttta ccatctttta ttgaaagttc ttttacaaga ctcattgctc
     1561 cctgataatc atacttatca gttggataat cgatatcagg tagtccaatc caactgacaa
     1621 tcagagcacg accatcagga gtattgaatc cttgagtcgc atacgcttca aatccataat
     1681 caagattttg aatttctgaa gcgcccacaa gttttacttt ttctatgtca agcgattgac
     1741 agatcttaaa agtattcgga tagatattcc cataatttag ctcagattta tccaaccctt
     1801 gaggacaata aatgagaaca ggttttttgt ctacaaatac tagatttgga cactcaatca
     1861 tgtattctga accagtgccg ccaaaatcaa ggttgccaac ttcctcccat gtttccacgt
     1921 tattgtcatc cgctttgtaa agtttgataa agcctgattt ttctaaattt tgtgctccaa
     1981 caatcgcata caattgtcct tcataattaa acatttgtgg atcacgaaaa tgatcagtta
     2041 catcagcagg tttatgaata agaacattct cacctttcgt aatgttgtag tctttatcca
     2101 tccatgctcc gatttgaagt gtatcacgtt cccaattttc atttcgaacg tttcctgtat
     2161 agagtaagaa tagattcccc tctatttcat atgcagaacc ggaataagcg ccgtggctat
     2221 ctcgttttgt gtctggaaga agttctgctc ctgttacatg aaaatgaact aaatcatctg
     2281 attcattgtg tacccattct ttcaacccat gagcagctcc aaatggccaa ttttgataaa
     2341 aaagcgtata ttttccgtta aaaaaagaaa aaccattagg atcgtttaac aatcctgttt
     2401 taggttcgat atgatagcta gcacgccaag gtgatcgttt tacattatcc ttaattttat
     2461 ccatctcatc tttagtccaa tcagaataag cacgatatcg tatttctcga ggtaagttca
     2521 tattttactc cttcaattta tctttttaca actagtatgg taaacgtttt ccgttgaaaa
     2581 atcaatcatt tcatttaaaa aaataaacta tatgacaaac gtttgacata tttttaagat
     2641 atgctaaaat aaatttgcat ttcagtgaac cgttttcacg taaaaataaa aaaggagtga
     2701 ttcgaatgaa ttacagtaaa gtagcttccg acgtaataca agcggtcgga aaagataacc
     2761 ttattgcagc tgcacactgc gctacacgtc ttcgtctcgt actaaaagat gacacgaaag
     2821 tgaatcaaaa agctttagac gaaaatccag atgtgaaaag cacatttaaa attgatggac
     2881 aatatcaagt gattatcggt gcaggtgatg ttaatttcgt ttatgacgaa cttatcaaaa
     2941 aaactggact ttcggaaatt tcaacagatg atttaaaaca aattgctagt aaagataaaa
     3001 aattcaaccc cattatggca ttaattaaat tattatcaga tatttttgtt ccaattatac
     3061 ctgcccttgt tgcaggtggt ttattaatgg cattacaaaa ctttttaaca gctgctggac
     3121 tttttggacc aaaatcaatt gaagaaatgt atcctgcgat tgaaggtatt tcctcaatga
     3181 tccaactgat gtccgctgca ccattcatgt tcttaccaat tttagtaggt atttcagctg
     3241 ctaaacgttt tggagcaaac caatttcttg gtgctgcaat tggtatgatc atgacaacac
     3301 cagatcttgg tggctcaact gaatattgga atatttttgg ttatcatgtt tcacaaacaa
     3361 actatgctta tcaagttatt ccagttttag cagctgtttg gatattatct atattagaaa
     3421 aattctttca taaaaaattg ccatcatcta ttgactttac attcacgcca ttattatcag
     3481 taatgattac tggatttttg actttcacag ttattggccc tgtaatgtta ttgttgtcta
     3541 atggaattac agacattatt gtttggctat ataacactac tggatttatt ggaatgggta
     3601 tttttggagg aacatattca cttatagtca tgactggttt acatcaatca tttccagcta
     3661 ttgaaactca attactttca gcgtggacaa atggtatcgg acatggagac tttatctttg
     3721 tagttgcatc tatggcaaac gttgctcaag gtgctgcaac atttgccatt tggttcttaa
     3781 ctaaaaattc taagacaaaa agtcttgctt cctctgcggg tgtctcagca cttcttggaa
     3841 ttacagagcc tgccttgttc ggagttaact taaaataccg ctttccattc ttttgtgctt
     3901 taattggttc aggaattgct acagcagtag caggattact taaagtagtt gctgtttcac
     3961 ttggttcagc aggtttcttg ggatttcttt caattaatgc aacatctatt ccattttacg
     4021 tattgtgtga attgattagc tttgtagttg cttttgcaat tacttacttc tacggtaaaa
     4081 caaaagctgc tggtatcttt aaagcagaag aaattgctga acaaacacca gttaattctt
     4141 caaaagtagc aacaacagat ccaatatcta ctgatgttga atcaacacaa acaaaagttt
     4201 catcagaaac aattgttagt cccatttctg gtaaagtgat tagtctttct tcagtaaatg
     4261 atccagtctt ttcatcagaa gctatgggaa aagggattgc cattaaacca aatggaaaca
     4321 cggtccattc tccagtagat ggtactattc aactcacgtt tgaaactgga catgcttatg
     4381 gtattaaatc aaataatggt gctgaagttc taattcatat tggtattgat actgtatcaa
     4441 tgggaggaaa aggatttaca caaaaagtta ctactaatca aaaagttaga aaaggcgata
     4501 ttcttggagc atttgaccgt tcaatgattg agaaagaagg tcttgatgat acaacaatga
     4561 ttattgtgac caatacttcg gattattcag aagtaaatat agtagctgaa aatacagtca
     4621 ctgagggtac tgatttactt gaaatcaaat aaatgtattt ccctgagtaa agatggaatt
     4681 ttctttc
//
LOCUS       DACCXO010000152         4658 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.159, whole genome shotgun sequence.
ACCESSION   DACCXO010000152 DACCXO010000000
VERSION     DACCXO010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.159"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            59..174
                     /gene="rrf"
                     /locus_tag="IVY70_002662"
     rRNA            59..174
                     /gene="rrf"
                     /locus_tag="IVY70_002662"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(227..697)
                     /locus_tag="IVY70_002663"
     CDS             complement(227..697)
                     /locus_tag="IVY70_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP7959753.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(856..1314)
                     /locus_tag="IVY70_002664"
     CDS             complement(856..1314)
                     /locus_tag="IVY70_002664"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP7959754.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1485..2629)
                     /locus_tag="IVY70_002665"
                     /pseudo
     CDS             complement(1485..2629)
                     /locus_tag="IVY70_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            complement(2642..3559)
                     /locus_tag="IVY70_002666"
     CDS             complement(2642..3559)
                     /locus_tag="IVY70_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP7959755.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3987..4550
                     /gene="efp"
                     /locus_tag="IVY70_002667"
     CDS             3987..4550
                     /gene="efp"
                     /locus_tag="IVY70_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP7959756.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1438 a          869 c          898 g         1453 t
ORIGIN      
        1 tgacttcaaa tccagttttg agtgaacaaa atttactcaa taaaagataa caccacagtg
       61 tggtggcgat agcgagaagg atacacctgt aaccatgccg aacacagaag ttaagcttct
      121 tagcgccgat tgtagtgagg ggttgcccct tgtgagagta ggacgtcgcc acgcaaaggc
      181 aaaaagatga actagaaata gttcatcttt ttttattttt attaagttaa agaacggatt
      241 caattgcagc ggtcattttc aatggagaag tttgtggtgc aaatcgttcg attactttgc
      301 catttctatc aatcaaaaat ttagtaaaat tccattttat tgctttagtg acagtaccag
      361 gagcttcatt tttaagataa gtaaaaagag gatctgtatt ttctccattt acgtcaatca
      421 attgatgcat agggaaggta acgccataag tggttttaca agcttctgct gcttcagtcg
      481 ctgaatctag ctcttgttta aattgattgg aaggaaagcc taagataacc agaccttgtg
      541 tctgataagc ttggtaaagt tcttctaatt gtttaaattg aggtgctaga ccgcattttg
      601 ttgcagtgtt aacaatgatt atcggtttgc ctgcatattt tttcaatgaa tagacagtgc
      661 cgtcttctaa agtagtttca aaattataaa tagacataaa gttttctcct ttctatttta
      721 tataataaaa agagcgctaa actgaaaact agctatttgt tttcccgttt agaactcttt
      781 tcagacaata aatatttaat aaaatacgaa gataagatag ctatattgct tctcatcttt
      841 acgttctttt agaaactatg cttctacttc agaccattca tcgcgttttg ctaaaaattc
      901 acgagccaaa gcttgtgcac cttctagtgt atggctggca gcccagccac attgtgtttc
      961 attgcttgca ggtacttcag tagcttcaag aacatctttc attgttgctt caagaacatc
     1021 taaaatgtct tcgtagcttt catgattcaa tacagtcaag taaaagcctg tttggcatcc
     1081 cattggagac cagtctacga tgtaatctgc gtgattgcgg attagttcgg cagtcaagtg
     1141 ctcaagtgaa tgtagacttg gcatgtccat gtgttcttta tttggttgtt taaagcggac
     1201 gtcgtattta atgatgacat cgccatgcgc acccgttttt cgatcagcta cacgtacata
     1261 aggtgctttg acttttgtat ggtcaagatt aaaactttcg acattcattc tcatttgtaa
     1321 gactcctatc tgtttgttca ttcgttttga tgcttttcac taaagaatta tttagtgaaa
     1381 tttacaaaaa cgtctttcta cttgattcta acacttatcg tttccaaggt gtagcaatct
     1441 gaaatatttt ttagattttt ctgctaagtc gtttattggg acttctagtt cttgattctg
     1501 tcaaatgcct gagctaaatc gtttatgaga tcctgaccat actcaattcc tacagaaaga
     1561 cggatcaagc cgtcctttat gccagcctct tctcgttttt cttttggaat agacgcatga
     1621 gtcatgacag aaggaatttc cactaaactt tctacgccac ctaaactttc tgctaaagta
     1681 aatagttgga gtgattcaac aaatggaata gcagattctt cattttttaa agtaaaagaa
     1741 atcattccgc taaagccgct catctgctgc ttagcaactc catggagttc atgatcgggt
     1801 aatcctggat agtagacctt ttctactgct ggatgagaaa atagaaaatc agctaccaca
     1861 aaagcatttt tttgatgttc ctccatacga acacttagtg tcttgattcc gcgctgaagt
     1921 aaccagctat cttggggacc aagtacggct ccgatagcat tttggtaaaa tccgatctga
     1981 tcggctagat cttttggtta gaagtcacta aaccagctac tacatcgctg tgtccgccta
     2041 gatatttggt accgctatgg acaacgatat cagaaccgag atctaaaggt ctttgaaaat
     2101 aaggagtagc aaatgtattg tcagcaataa ctatcaatcc atgttgatga gccagagtgg
     2161 cacttttttc taaatcagta atctttagaa gcggattgct aggtgtttca agataaagtg
     2221 cttttgtatt gggtttgatg gattgttcta ttttatcaag attactcgta tctatgatcg
     2281 tgtactcgag accattttta gtaagaactt tgtcaaatag acggaatgtt cctccataga
     2341 catcatctcc taataagata tgatcgccag cttgaaaaag agaaaaaacg gcatgaatcc
     2401 cagaaagacc agaagaaaaa gcgaatcctc gaacaccgcc ttccaaatca gcaatcagtt
     2461 cttccaatgc aaaacgagta ggattaccag atcttgaata ttcatattgt ttaggctggc
     2521 cgacgccatc ttgccgataa gtggatgttt gataaatagg tacactgaca gctcctgttg
     2581 tgggatcttc actgatgcct ccgtggatca attttgtttg aatgtgcatg tttctttcct
     2641 cctataagta aatgtttttt gataaatagc ggtctgctgc gtctggaaaa atcgtaacga
     2701 cacgatgacc aggtggtaaa cgacgaattt ctcttaatgc tgctgcaaaa gccgcaccac
     2761 tcgaacttcc aactaatagt ccttgttctc gtgctaactg cctagtatag ttaaaccctt
     2821 ctacatcaga gattgtctcg atctgattga ttgaaagggg agaaagaaac ggcggaatga
     2881 attctacacc gataccctca atttcgtgag gggctggatc accaccattt aacacagagc
     2941 cctctggttc tacaccgatt attcgaatgt cgggatattt ttctttcaaa aaggtagaag
     3001 ttccagcaaa ggttccacca ctcccaattc ctgctacaaa actatggata ttttctttga
     3061 tttcatgaaa gatttctggc cctaacgtat gatagtaagc agcaggatta tctttatttt
     3121 caaattgaag aggcagatag ctgtttgata tctcctctgc aagtttttta cttttgctga
     3181 tggcaccgat gattccttgc tcactaggag aatggatgat ctttgctccc aatgctttca
     3241 tcaattgttg tttttctaag ctaaacttct ctggaacaac aaaaaccgtt cttaatttgt
     3301 atgtgagtgc ggccagagcg atcccaattc ctgtatttcc ggcagttggt tcgatgatcg
     3361 tcgttttttc attgattttt ccttgggcaa atccttcttt gatcaaataa ctgcccaatc
     3421 tatctttgat acttcctcca ggatttaaat attctagttt tgcataaata gcagaacctt
     3481 taggcataga atagtgctga ttgtcaaagc gataaagcgg tgtggaacca atagcttctg
     3541 taattgtcga gataatcatg tgggcctcct aaaaaaagat aataaaaaaa ggagctgact
     3601 attaaatcag ctccttgaaa gacatagaga aaaattgagt agataaaaag ataagttacc
     3661 tggtactcat tctatgaagg gcaattaata gacatgacaa atagtatgct gcttttatgc
     3721 accatacaac agcaacgatt caattgtttt tggttgctga ctgtcatgtg tatccctcca
     3781 agtaaaattt ttattaacga tagcatgaaa aaaaataaaa gtcaaaggct tgaaagaaaa
     3841 aaatatttcg atagcatcgt ttacccgtgt ttatttttct gacaaatagg catgagacct
     3901 taaaagatag agaatactct agttttctgg ccggttgtct gatacaataa agttgattat
     3961 gataaagaaa agaaggagca aaaataatga tctcagcaag tgatttaaga gccggtatga
     4021 ctttcgtaca agacggcaaa ctgattaaag ttctggatgc aagccaccat aaacctggta
     4081 aaggaaatac agttatgcgt atgaaactga aagatgtccg ttcaggtgca acatacgaca
     4141 caacgttcag accagaagaa aaatttgaaa aagcacatat cgacacaaaa cctgtacaat
     4201 atttgtacac gatggatgat acagctttct tcatggattt ggaaacatat gagcaatatg
     4261 aaattcctgt agaaacagta gaagaagaaa tgaaatatat cctagaaaac atggaagtaa
     4321 aaatccaatt tttcggtagc gaagtcatcg gcgttcaatt accaacaact gtcgtattac
     4381 gtgttgtgga aacacaacct tccatcaaag gtgcaacagt cactggttca ggtaagccag
     4441 cgacaatgga aactggctta gtagtcaatg ttcctgactt catcgaaaca gatgaattgt
     4501 tggaaatcaa tacagcagaa ggttcttatg taaaacgtgc gtcaaaataa ttcgctaacg
     4561 gattgataag aatttgaaaa cagagcttga cctcttgaaa ggcagatcat cggatctgcc
     4621 tttcaagagg ttttttgtgg aatcagcaaa ttgaaaaa
//
LOCUS       DACCXO010000153         4633 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.160, whole genome shotgun sequence.
ACCESSION   DACCXO010000153 DACCXO010000000
VERSION     DACCXO010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.160"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(89..751)
                     /locus_tag="IVY70_002668"
     CDS             complement(89..751)
                     /locus_tag="IVY70_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7959757.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            935..2581
                     /locus_tag="IVY70_002669"
     CDS             935..2581
                     /locus_tag="IVY70_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP7959758.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            2572..3654
                     /gene="uxuA"
                     /locus_tag="IVY70_002670"
     CDS             2572..3654
                     /gene="uxuA"
                     /locus_tag="IVY70_002670"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP7959759.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(3762..>4633)
                     /locus_tag="IVY70_002671"
     CDS             complement(3762..>4633)
                     /locus_tag="IVY70_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7959760.1"
                     /translation="FPATSVKDVQKEFTEYEYIDFFIDLSVDILRPNLPICREQRILV
                     NILARLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESLCTVFDLFIPEKEKILY
                     SLAFARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLP
                     VILEKENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIK
                     IQNDFMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEA
                     LMDQSKKENQVTS"
BASE COUNT         1483 a          799 c          911 g         1440 t
ORIGIN      
        1 tgcaaagtca atggatattt tgttatttaa taaagattac tcaaaaacga aggttaatta
       61 taaaaataga agaggtcgca agaaaatgct actgctggaa atactcggga taacgttgag
      121 taacagtttc ctgttctttc agcatcatgt gtaagtgcaa tcccgtttga aatcgagctt
      181 cttctggttt tttatcaact aaagccttga gtaaatcata atgttgttcc atgatcgttt
      241 cccatggaag cccctgaatg gtcactcgta accagcggaa acgttctaaa tgggtattat
      301 ggtcatctaa ccagttccaa acttcttttc ttcctgcaat ttcgaaacaa gcttcatgaa
      361 ataaattatc tgcacggaaa aaagcgcgtt cgtcatttat tttgactgct ttttcttgtt
      421 cttctaaaat cgtttctagc actattcgtg tttgtgtgtc caatttggaa caacattcaa
      481 tcatgatttg ctcttccaac tgttctcgga caaagcgagc attttctgca gaaacaagat
      541 taattttgga aacatacgta ccactttgag gtatcgtata cacgagttct tgctgacgta
      601 gttgaatcaa tgcttcgcga atgggagtcc ggccgatagc aagatgttcc tccagtgcct
      661 tttcagatat tttccttcca ggttctaagt cagcatagat aatttgttta cgaatcgctt
      721 gataagcttg tgattgcaag ctgcttttca tctttattcc tccaaaatca tttctatgaa
      781 cgtagtataa cagaagaggt ctgaaaaaca atctggtatg tcacattaac tagtaaattt
      841 gtataaatag taaaattttg gttgacatag cgatttttat tgcataaaat tcacttgaat
      901 acaaaaacta atatgttagt ttaggagggg aaaaatgatt gatttaaaca actggaggaa
      961 ctttatccct gaattatctc aaaaagaagt agaaatacct cattttgacc gtgcatcatt
     1021 aagagaaaat ggacaaaaaa atcctgtctg gatccatttc ggtggcggaa atctttatcg
     1081 aggatttcat gcagaaatcg ctcaacgttt agcggatcta ggcgaattgt ccactggtgt
     1141 cattgtttgt gaaacattcg atgaagaaat aattgaaagg gcttaccaag attttgatga
     1201 tttgattctt gaagtggtca tgccaagcag tggagagttc gtcaaacgta tattagctgc
     1261 gacagctgac agtctgtatt gtcatccaga ttgttctgaa cactttgata agatgattcg
     1321 acactttgaa aatcctagtt tacaattagt tacttttgct attactgaaa aaggttatcg
     1381 attgaaagat acaaataata aatatatagg accgattgca gtcgatataa aaaatggtcc
     1441 aaagaagcca tcacatacaa tgagtattct tgctgtgcta ctttataaac gttttttatc
     1501 tggagcttgg ccgattgcac ttgtctctac agataatttt tcacaaaacg gtagtcaatt
     1561 acgtacttct ttgctaacga tcgcaaaaga atggcaaaag gaaggttatg tacaaaatac
     1621 atttatccaa tacttatcga atgaagaaat tgttgcgttt ccatggacga tgattgatcg
     1681 tatcaccccg aatccttcct tgtcagttag caaaatgttg gcaaaagagg gcttttcaaa
     1741 tatgtcgatt ttccaaaccg caaaaggaaa taatattgct ccatttgtca atacagagga
     1801 catccattat ttaatcatgg aagatcattt tcccaatgga cgtccgaaat tcgaaaaagc
     1861 tggggtattc ttgactgatc gtgcaacggt agataaagca gatacgatga aggtgacgac
     1921 ttgccttaat cctttgcata cgtctttagc tattatgggt tgtttactgg ggtataggag
     1981 catagctgag gagatgaaag atccagattt atcgctgcta gtttctgaaa tcgggtacaa
     2041 tgaaggcctc ccagttgtgg agtctccagg tatcattgat ccaaagatct ttctggaaga
     2101 agtaattact gtgcgctttt ctaatccaaa tattcctaat atgcctcagc gaatcgccac
     2161 ggatacctct caaaaggtgg cgattcgtta tggtgagacg attaaaaaat atatggaaat
     2221 agaagttaaa gacccgcaga cacttgtatt cataccattg actctcgctg cttggcttcg
     2281 ataccttatt ggcattgatg atcaaggaaa accattcatc ccgagtccag acccactatt
     2341 gacagacctt cagcataagc tttccgatat tcaactgggt acgcaagata catcacatat
     2401 tcatgagaaa atggctccta ttttgtcaaa taaagaaatt tttggtgtag atctttacca
     2461 agcagagctt ggcagaaaaa tagaagaaat gttcatcaag atggtagcag gaccaaatgc
     2521 cgtacgaaaa acattacagg attacttaca agaacatgga ggaaatttat catggaaatg
     2581 acttttagat ggtacggatc acaggaagaa aagattacct tagatgaaat cagacagatt
     2641 cctgcggtca aagggattgt aggcacattg atggatatac cagtaggaga ggtatggcct
     2701 agggagagga tcaaagaact aaaagaagaa attgagtctc gtgggttaac actaaaagta
     2761 attgaaagcg tcaatgtgca tgatgccatt aaaattggat taccttcccg tgagcagtat
     2821 atcgaaaact ataagcagac aatccgaaac ttggcagaat atggaataga agtcatctgc
     2881 tataatttta tgcctgtatt tgactgggtg aaatctgacc ttgactaccg cttagaagac
     2941 ggctcatcca cgttagcttt tatatctgat gatattccag atgatccaag agaaatcgtc
     3001 gcgcgcatcg aacattccag caatgaattt ggattgccgg gatgggaacc tgagcgctta
     3061 gctcctatca aatccttgct tgaagcctat gcatcagtag acgaagaaaa acttcgagaa
     3121 aattttgcct attttctcca atcaatcatc ccaacatgtg aagaggtagg ggtcaaaatg
     3181 gctgtacatc cagatgatcc gccatactcg atttttggat tagctcgaat cgtaaagaat
     3241 cgagaagatt tagattggtt gtgtcgcata gtagacagcc cttataacgg aatcactctt
     3301 tgtacaggga gtattgcaga agatccagaa aatgatgtat atgggatttt agcagaattt
     3361 tgccaaagag atcgaatccc atttgcacat gttcgaaata ttaaattttt agctaatcga
     3421 gatttctatg aggcaccaca taaatccgaa tacggttcat tagacatgta ccgcatacta
     3481 aaaacaatgt atgacaacgg atttaacgga tacatccgtc ctgatcatgg aagaatgatc
     3541 tggaatgaga caggaagacc aggatacggc ttatatgatc gagcattagg tgctgtttat
     3601 ctaaatggat tatgggaagc aatcgaaaaa ggaaacaaaa gtaaagataa ttagtgaaaa
     3661 aaactgtaat tttattatgt aaataagaag gatgttcaaa ttcttgatac ataaatccgg
     3721 aggttgttaa taagcattcg gatttatgta tcttatttgg tctaggaagt aacttgattt
     3781 tcttttttcg attgatccat gagtgcttct tcttttaatt caaagatata ttctgagatc
     3841 ctcataatca cttttttcca gtcttctggt gtgatgattc cttttaaaat atagattttt
     3901 ttgatatgag aaggcaattg tttcaaaggg cagtcgctga taacgatgtt gcagttctcc
     3961 ataaaatcat tttgtatttt gatcgattca tttgaaaaaa tttgttctat cttaacgatc
     4021 agatctttac tgatataaaa atcacgcgta tagttcacac caatcacaat tcgcggctgt
     4081 tttttgtagc gatcatacaa atgtactagg tcttcaacca tccattgcac attttccttt
     4141 tctaaaataa ctggtaacaa gtgttgattt tgttctctga aatccatata aaattgttca
     4201 atggcggttt ggacttcttt caatacgcta tgttcttgat aaagcatgaa agtaggaagg
     4261 ggcgtatttg gttgattgaa gttaagaaat ccattataaa gtttatttcg tgcgaatgcc
     4321 aatgagtaaa gaatcttttc tttttcaggt ataaacaaat caaatacagt acaaagggac
     4381 tccaccattt ctcttgaata aagaacatga ggaacgtttg ctttttttaa aagtttgtat
     4441 tgctccaagc ttgtttcttc cgattcgatt ttcgtgatag aaagtcgagc taaaatattc
     4501 actaagatgc gttgttcgcg acaaattggc agattaggtc gcaaaatatc tacagagaga
     4561 tcaataaaaa agtcaatata ttcatactct gtgaattctt tttgtacgtc tttcacagaa
     4621 gttgcaggaa aat
//
LOCUS       DACCXO010000154         4565 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.161, whole genome shotgun sequence.
ACCESSION   DACCXO010000154 DACCXO010000000
VERSION     DACCXO010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.161"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            191..2035
                     /locus_tag="IVY70_002672"
     CDS             191..2035
                     /locus_tag="IVY70_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7959761.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            2091..3557
                     /locus_tag="IVY70_002673"
     CDS             2091..3557
                     /locus_tag="IVY70_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7959762.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            3594..4436
                     /locus_tag="IVY70_002674"
     CDS             3594..4436
                     /locus_tag="IVY70_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7959763.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
BASE COUNT         1468 a          746 c          990 g         1361 t
ORIGIN      
        1 ttgaatataa cttaaacagg catgtgactg gtaatgcagg caggacctaa ataagttgaa
       61 taacaatgta tgcgtttcaa tacacgtttg ccttgttgtc aatttattta gtttctgcct
      121 gttttttgtg ttgaggatgg ccacttcgac tcaaaagttc atcactcaaa aaagaaaaga
      181 gggaaacacc atgaattaca aacaaacagc agaagaagta ttgcagtaca tcggtgggga
      241 agaaaacata tcccatttgg agcattgctc aactcgttta cgttttacgt taaaagacaa
      301 cagcaaggta aatatgagcc agttagaaaa agtggatggc gttatgggcg ttcgccaaaa
      361 tgtgcaatgc caagtaatca ttggaaacga tgttgttgaa gtgtatgatg aagtgaaagc
      421 gttattaggt gacaatgttt caaatactga agaagcacca aaacaaaaac aaaaattagg
      481 ttcagttcta ttagatttta ttatttctat tttccaacct ttaattcctg cgattgcagg
      541 tggaggagta ttgaagtcct tgttgctact agcttctatc ttagggctga ttagtgacca
      601 atctcaaacc tatcagattt taaatttggt cggcggggca ccgctttatt tcttgcctat
      661 tctggtggca atgactacgg ctaataaatt gaaagtcaat caattggttg cagtatctgc
      721 tgtcggtgca ttgatccttc ctgaaatgac agctatgttg gcggaaggtg ctacattctt
      781 atcttttgga ttggataata tagcgtatgc atctcaagta ttcccagcca tcttaactgt
      841 tttattttat gctcaacttg aaaaatggtt tacaaaagtt tcaccaaaac caattcgtat
      901 tttctttgta cctatgatga gcttattgat tacagttcct attgctttgt tgcttctagg
      961 accattaggt tataatgtag gaacagcttt ctcttctatc attgtgtctt tgtacaatca
     1021 atttggatgg gttgcaaccg gagtcttagc agctgtactg ccattaatgg ttgtaacggg
     1081 tatgcataaa gcaatgatcc cttacgctgt ttcatccatg agtgaattgg gtgcagaact
     1141 tctttatctc ccggcttcat tggcacacaa tatttcagaa gctggcgcat gctttgctgt
     1201 cagtatcaaa acaaaagaca aaaaattgaa atctacggct gtttctgcgg gtatatctgc
     1261 attattcgga attactgaac cagctcttta cggggtaacg attctgcaca aacgtgtttt
     1321 atatgccgtt atgttttcta gcctgatcgg gggagcattt gcaggaatcg ttgcacttca
     1381 agcctttgct ttagtgggtc ctgggattgc aagtatcacg atgttcgctg acaaagctga
     1441 cccaatgaat ctggtttggg catttgcagt atcggggatt tcatttgtgg ttgcttttgc
     1501 tgcagcgttg gttctttata aagataaaga agttgtcaat gaagttgaaa ctaagatgga
     1561 aacggccgtt gtttcagatg ctgttctttc aagtcccgtg aatggaaaag taatctcact
     1621 atcagaagta aaagacactg ttttttctag tggtatggta ggtgatggag tagcaatcat
     1681 tcctgaagaa gggatattag tagctcctga agatggagaa atcacaatgg tatttgaaac
     1741 gaagcatgct attggtttaa aaacggctaa tggtgcagaa cttctttttc atattggtat
     1801 tgatactgtt cagttagaag gaaaagggtt tgaggcattt gttcgttctg gtgaaaaagt
     1861 agccaaagga caaaagctga ttgcatttga tattgattta atcaaagaag caggattcga
     1921 ccccacagtt atctgtgtgg taaccaataa ggataattac actatccgtt catttaacct
     1981 agaaaaattg gtaaccaatg atagtgatct cttagctatc agtccgatag cttaaacaca
     2041 agatagtgaa aaaaaatata ttgaatgaag gaatagagga gaatagaata atgacaaaca
     2101 caacaagttt tccaaaagga tttttatggg gcggcgcgat agcagcaaat caagtagaag
     2161 gtgcttggaa tgtagacggt aaaggtcttt cagtagcgga tatggctact tataaaccaa
     2221 atttagacaa caaagatttt gctgctcata tgcatgtcac cctgcagtct ataaaagaag
     2281 ctagtgaaga caaatctgat aaatggtacc caaaaagaag gggcgttgat ttttatcatc
     2341 actataaaga agatttagct ttatttgccg aaatgggctt taaaaccttg cgcctctcta
     2401 ttgcttggac tcgcttgttc cctacaggag aagagctgga accgaatgaa aaaggggttg
     2461 cattttatga aaacgtcttt aaagaaatgc aacgtttagg aattgagccg attgtgacac
     2521 tatctcatta tgaaatgcca ttagctttag cagttaaata taatggctgg gtggagcgtc
     2581 gagtaattga tgattttgtt cgcttcgcta acgtttgttt tgatcgcttc ggcaaatacg
     2641 taaaatattg gctgacattt aatgagatag acagcatcca ccgtcatcca tttacaacag
     2701 ctggaattat ccaagagaaa agcgcagaag gtcaagaagc tcaagatatc tatcaagcat
     2761 tgcatcatca atttgttgcg agtgcgttag tgacaaaaga tgctcatgaa aaaattccag
     2821 gaagccaagt tggttgtatg ttaacgaaat tgatgactta tccattaact tgcgcaccag
     2881 aagacgttga gctgaccttg aaaaagaact tagaaaataa tttttatgca gatgttcaag
     2941 ttaagggaga atatcctgct ctaatcgaaa aggatttgga aaatcgggga attcagatta
     3001 aaatggaaga tgaagatcga gaaattttaa agaaacatac cgtagatttt gtttctttca
     3061 gctattacat gtccatggca gaatcagctg atccaaacgc agaacgcaca ccgggaaata
     3121 ctgttttagg ggttaagaat cctcatttgc cttcaacaga ttggggctgg caaatcgatc
     3181 caaaaggttt gaaaatttcg ttgatcgaac tttatgatcg ttaccaagta ccgttgatga
     3241 ttgtggaaaa tggtatggga gcagtcgatc aggtagtaga tggtaaaatc catgatccgt
     3301 accgcatcga ctactttaaa gcacacttcc aacaaatgaa agaagcaatc gatgaaggtg
     3361 tagatttgat agcttatact agctggggac caattgattt agttagtgca ggaacttctc
     3421 aaatgtctaa acgatatgga tttatttatg tggatgcaga cgatgaagga aatgggacat
     3481 ataaccgctt gaaaaaggat tcgttcaact ggtacaaaga agtgatcgaa acaaacggtg
     3541 catccttatt cgagtagtct cgatcttttt aagcagcgat agggggataa tgagtgattt
     3601 caataaaaaa agtattgaat tcaagtgtgg tacttgttga agaaaatggc aaagaaatga
     3661 ttgcattagg taaagggata ggctttggac agaaagctgg ggatgaaata tcaggagata
     3721 aagtagataa aatatttctg ccttttgaag aaaagaaatc ttcacaacta ttagagttag
     3781 ttgaagaaat acctattgaa ttttttgaaa tcactcgtac tatcgttttt gaagcagaaa
     3841 aaggattaga taaaaaactg aatcctggag tctatttaac cttaacagac cacctgcatt
     3901 ttgcagttga acgcgcacaa aaaggggtaa atattgcaaa ccgtttgtac tgggaaataa
     3961 aaatctacta tcccaaagaa tttcaaattg ggaagtacgc actcaccctt ttgaacgagc
     4021 aatatggaat tgatcttcct aaagaggaag cctcaaacat tgctttccat ttgatcaatg
     4081 cacaatcgga agatcctgat agcggcgctg gattcaagta tgcaaaaatg atcggcggga
     4141 tcgtcaattt ggtccggtat tcgttgcaga aagatatcga tactaattcg atccattatg
     4201 cccgcttcat tacacatgta cgtttctttg tagaaagata ttattctgat gctttgttgg
     4261 atgatggtga ggaagaattc tacaatcaaa tgtggatctt atacccgtct gccatggaaa
     4321 gcgcagctaa gatcaaagac tacattgaaa aagtttatca taaaacaatt ccgaacgatg
     4381 aagttgtata cctaggtgtc cacatcaatc ggttgatgaa acatgctaat gggtgagcgc
     4441 tcagtaatac tattgatgct tttaaaatct ttagagatgg attatagtaa aaacaaggtg
     4501 atttccagct gtttgttatc tataagagac atttaaaaag gattaacgta agataataaa
     4561 aaatc
//
LOCUS       DACCXO010000155         4440 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.162, whole genome shotgun sequence.
ACCESSION   DACCXO010000155 DACCXO010000000
VERSION     DACCXO010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.162"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            276..1007
                     /locus_tag="IVY70_002675"
     CDS             276..1007
                     /locus_tag="IVY70_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7959764.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1152..3179
                     /locus_tag="IVY70_002676"
     CDS             1152..3179
                     /locus_tag="IVY70_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP7959765.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            3287..4384
                     /locus_tag="IVY70_002677"
     CDS             3287..4384
                     /locus_tag="IVY70_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP7959766.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1551 a          720 c          921 g         1248 t
ORIGIN      
        1 ttttacactc tcgtcaagtc acattttgat agataggagg gggacttacg aattatttta
       61 aaggctatga cagctgtatt tctagattgt gtttgcatca agttgcaata aaaactagaa
      121 atcaaatcgg gctgataatc atttataact aatcgataca taggaccaaa tataggtcta
      181 tgttctttta tttcataagt atcgtaaaac agaattttgc tatggattaa tccaatcgac
      241 tgatatgttt agtaaataaa gaaagaggaa tgtcaatgat gaaaaaagaa atcgtattaa
      301 cagcagcaat gatgttattc agcatggtag caagtacgac ttttgtttct gcaactgaag
      361 tatacccaaa ggaatataac acagaaggaa cgattacatt tgaagcagga gatgagggtg
      421 taactcctcc tgttgatcca gagaatcctg atcccaataa acctgtcgat ccatcagatc
      481 ctccaagccc tggaacagga ggggcattat cgattgatta tggatcaaaa ttcaaatttg
      541 ggactcaaaa aatttctact gcggataaaa cgtattatgc agctgctgat gtgatgaacg
      601 atgggtctag aaaaccgaca tatgtccaag tgacagatag gagaagtacc ttatctgggt
      661 ggaaattaag tgtctctcaa ccagaacagt ttaaaactgc cagtggggat gaactagttg
      721 gagcacagct gaaatttacg aaagggcagg ctgtttcttt ggttgatcca acatatacgc
      781 cgcaaacggt gaattctgaa ttaacgttga caccgggagg aaacaatacg ttagcaatta
      841 atgctaaatc aggaacaggt gtggggactt gggtctatcg atttggtgca aatgaaaatg
      901 aaaatcaaga tgcggtacag ctttcggtac ccggtaaatc tgtcaagtta gcgcaacaat
      961 attccactaa attagtttgg acattagaag atacacctaa caactagttt actgaaaaga
     1021 actgttaaaa ataaatacgt agtcatattg tcttgcgagg taatcgagca aaaattgtca
     1081 aaggctaggc taaatttgtt tggatacgaa tagttcatct aggaatttaa cgatcaggag
     1141 gaaaagatat attgaaaaag aggaggatcg tgctcatttt aagcagtctc ttagtgacta
     1201 atatctttcc gatcataggg tactctttgg aattgaatca gttggagcat ggaaatagaa
     1261 gagacgaaga aagcaaactc acttcgacat tcgacagttt aatagaatcc aaccagacac
     1321 aagaaagcag tgaggattta agtatttcga gtagtggaga gaccactgac aacgtagaag
     1381 ctgagggttc ggacaaacaa ggactagaag atgcaccaat ttctgcggtt gagctgcctg
     1441 acgatcccaa tattatttct atcgataaaa tttttcaaac acctatcggt actagcacgt
     1501 ccatattgga aaatgggaag ttactgcagt tgaacccagc agaaaggtct caaagaggag
     1561 ctatttggtc aaataagaag attagtttac tctcggattt tacatttaaa tcttatttat
     1621 acttaggaaa tcaacgagat agagcgggag atggcatgac ctttacattg actggcgatc
     1681 ctagaatggc tagtaatccc agtcaagtta ttggatcgcc aggtatggga attggctcat
     1741 attctactcg ttctggccaa ccttacattg cgaatgcgct ctcgattgag tttgatacgt
     1801 ataaaaatag tggttcttct gatcgaatgg atcgagagat aactgctgat aatagagggc
     1861 atggacatgt cgcttttgtc acgcctaaac caaataataa taattattct ggagagcata
     1921 ctggcgtgac ggtagctcct acatttcttt ctaatggcac atggcgcttg ttaataatta
     1981 aatgggacgc ggagcaaaga agtcttactt atgatttgga aggtgtcggt cgctcttctc
     2041 ggattatacc tgatctcatt aggcagtttg gttctacaga agtaaattgg gggtttactt
     2101 cttcaacagg tacttttttt caagaaaatg ctgtcgctgt cacgcgactt ccatcgaatg
     2161 tcacatcaac agctgatgta aaagtcaacg atagtgatta tggggccatc gctgaagctg
     2221 gaggaaacga taagataact atcagaaata cattgaaggt cgagggggat tttcttgaat
     2281 ataagaaaat gcagactagt atcgacctcc cacaagaatt aactattccc gagcatatct
     2341 tgatcaatgg aaatcaaatt gatgccaaag atatcttgat aaccggaaat cgagtcacgc
     2401 taaatctgac agattatttt attgctggag atacgttaat aatcgaattg gagacaactt
     2461 tgcaggaagt aattcctgaa aaagtactca ctatgcattt tgaatacctt gaaaacggga
     2521 atttgattga gcaatcgaat gaaataaacc taaagatcgc aaagctgaag gagatagata
     2581 tccatgttta ttataaagat aaagacagtc agattgatat tgctgaaatg aaaacaatca
     2641 ctgggaaaat aggagagaag tataaagaaa ctcctattga tattgaaggg tatgtttttg
     2701 actcggattc agcgaataca gaaggcatct tctccgaaaa tacagaggat atctattttt
     2761 attacagaaa aggcaaactt tatctctcaa ctgctcctca atatgtcaat tttggaaaac
     2821 ataaaatatc cagtagacca ttaagtgaat tcggacagtt ttcaggtggg ttgaaaatta
     2881 tagatgaacg ggcttttgga agttggaaat tacaattgaa acaagagaaa gttcttacaa
     2941 atggagattt tgagcttcca gaagctcttt cctttgttac ctctgaaaat acaacggtta
     3001 tagggacatc cgctgttacg attttcgaaa gtgatcaaaa gggtgaaagc gatttgtcct
     3061 ctcttttaga ttctacagct catcgtggaa tcagaataaa tattcctgta gaaaaccaaa
     3121 gaatcggaac ctttgagggg aaactatctt ggatacttgc agatgtccca ggaaattaac
     3181 aggaagagaa gaatataaca aaattattga gtttcaagaa gggcacaggt accataggct
     3241 cttgtgtccc acagagacaa gattttgtta tagtcttctc ttacatatga ttaaaggagc
     3301 gattttcttg agaccaaaac aacaattatt tataacactc ctagttattt taggggcact
     3361 ttttaacaat atatcagtgt cagctagtgc atttaatttt tcagtgacgc cgataacttc
     3421 agagaatcaa atcgacaaga gaaaaactta cttcgatctt caacttgatc cggatcagga
     3481 agtagaagta aaagcggaat tgagaaatga tacagaaaaa gaagtgaaaa tcgatatttc
     3541 tgtgaatagc gcaaccacca attcaaatgt gatggttgaa tacggaaaaa acgagattga
     3601 aaaagacatg agcctgatct ttgatctggt agattatgtg agctatccac agatagtgac
     3661 gctaaaacct aaaagtgtcc aaaccgttgc ttttcatgtc aggatgccga atgagcgatt
     3721 tgatggtgta ttggctggtg ggatcacatt ccaagaaatc cagacggaaa aggaccaaac
     3781 agaaactaag gatcaaagct tatcgattga gaatgaatac acctatatta ttgctttgtt
     3841 gatgcaacag acactgaacg aagttgctcc gaaccttact cttcatgaag ttaaaccaga
     3901 tcaaatcaat gcaagaaatg tcatcttagc aaacgttcaa aatgatcaaa aaacctatat
     3961 caatcaagta gttattgaaa caaaaattac aaaaaaagga cattctgaag ttttatatca
     4021 agaggaaaaa gaaggattgc agattgcgcc gaatacaaat ttttcttttc caactgcttt
     4081 aaatggtcaa ccgctaaccc caggtgaata tcaccttaca atgactgttt taggaaatga
     4141 aaatgaaaat ggaaaatttt cgagaaaaaa aggaaatact acgatcaact ataccaatca
     4201 atgggtattt gaaaaagatt ttacaataga cggaaaagta gccaaggaat tgaacacgac
     4261 ggatgttacg attaaaaaag acaataattg gatttatatc ttagtagctt cacttttctt
     4321 attgtttatt ttattatgca tacttttgtt tgttttgaga aaaaaagaag agaaagaaaa
     4381 gtaaggttaa gataacaaat agtaaatttt tattattgta ggatggttgg gctcttttta
//
LOCUS       DACCXO010000156         4289 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.163, whole genome shotgun sequence.
ACCESSION   DACCXO010000156 DACCXO010000000
VERSION     DACCXO010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.163"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            742..>4289
                     /gene="esp"
                     /locus_tag="IVY70_002678"
     CDS             742..>4289
                     /gene="esp"
                     /locus_tag="IVY70_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP7959767.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVSLPE
                     DKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDIIENPGENIPAGYHKVT
                     FTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEF
                     VSSATKLDDIIENPGDNIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVSLPEDKLPVL
                     KAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDIIENPGENIPAGYHKVTFTAGEG
                     TSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATK
                     LDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKK
                     GTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT         1611 a          663 c          876 g         1139 t
ORIGIN      
        1 atcaaaaatt ggatcagtta attgaaagag aaagctggga agattattac gtgggtctaa
       61 ctttttcaga aaagttaaac aactacaagt taatagatta taagtatttg agtgaaagca
      121 attaattatt catgtagagt actagacact ttaaatatgg acaattcaat tataaagaaa
      181 aagaatcaat aatttaatga ggctgtgaca aatgtcattt tttgtttcgc taaaaacgag
      241 tgaaaaaatg ttgataactc caatcattcg tttaagattc taggaatcat tcagaaatgc
      301 tgtgaaaccg atactttata attataaaga aggtgatttc aaattccgaa gaaggaattt
      361 gaagtcacct ttttacttat gtcacagtct cattcagtaa tgcgggataa ggctccttat
      421 tccgttattt gaatactctg aagtttaatc taagaaaggg taatattttg aaaacatgct
      481 tcatcgcata gaaatgaact taatttcaga aagaatacat acatatcttt ttatatgttt
      541 tcagctgctg aatggttaaa aagcacaaat atccgaaaaa aacatgaaga aatgtacttt
      601 ttttgttgta atgttcatga aattctctaa gtaaattagt taacataagg ttagtaagat
      661 tcctattctg tgaataatac gtatcagatt tatcttttat tcttactagg aaagtaatta
      721 ttaagggagt tgatttgaat aatggttagc aagaataata agagagtatt tctagaaaaa
      781 acaaagaaac gagtattgaa gtacagtatc aaaaaattaa gtgttggggt tgcatctgtt
      841 ttgattggtg ttggtcttgt cttgggaaca actgaactag ttaaagcaca agatgaaatt
      901 tctccaagca gcccacttga aacagcgata agctccgtac aagtaggtga caaagtagct
      961 tcggggaata cttttcagga gaatcctggt tatacgaaaa attataactt ttcggattta
     1021 caatttagtc ctcaagaact aactggagac acactaaaag gcaatacaat tggatttgaa
     1081 gtttacggaa aacataatat tgcagcatca actaaaaact gggaaatccg tcttcaatta
     1141 gatgaacgat tagctaaata tgtagaaaaa attcaggtcg atccaaagaa aggaattgga
     1201 tcttctagac ggacctttgt aagaattaat gattcgctgg gtagacctac aaatatttgg
     1261 aaggtcaatt atattcgtgc aagtgatgga ttatttgctg gggcagaaac aaccgatacg
     1321 caaaccgctc ctaatggggt aattacgttt gaaaagagcc tagatgaaat ttttaaagag
     1381 ataggcatag acaatctcaa gaccgatcgt ttaatgtatc gaatctactt ggtaagtcat
     1441 caagacgatg ataaaattgt accgggtatt gatagtacgg gctacttttt aacagattca
     1501 gatgatttct ataatagctt agatgtatct gaaaataatc ctgaccagtt taaacatgga
     1561 tcagtaagtg caaaatatga gaaacctaat actcaaacaa aagatggttc aggatccaca
     1621 ggtgctaatg gagctataat cttagatcat aagttaacga aaaattataa tttctcatat
     1681 tcatcttccg caaagggcac gccttggtat gctaattaca aaattgatga acgattagtt
     1741 ccatatgtag cgggtataca gatgcacatg gttcaggctg acaaagtaac atatgatgtt
     1801 tcgtttgaat ccggaacaaa agtagctgat ttagcaatcg agcgacggga aggtcatgaa
     1861 aattatggca taggttcaat aactaataat gatttaacta aacttatcga ttttgctaat
     1921 gcaagtccac gtccagtcgt tattcgatat gttttacaat tgactaagcc attagatgaa
     1981 atcttagaag atatgaaagc aacagcacaa gttgaagaaa ataaaccatt tggtgaagat
     2041 ttcatctttg attcttggtt gtcggatacg aataaaaaat taatccagaa cacttatgga
     2101 acaggttatt attatttgca agatattgat ggtgatggaa accctgacga taaagaagag
     2161 agcggagaca cgaatccata tatcgggaaa cctgaattag aagaagtata tgatgttgac
     2221 acaacagtta aggggaaagt attcatccac gagttagcgg gaacaggtca caaagcccaa
     2281 cttgttgata aagaaggtac tgtattagca gaaaaaacta tcgctccaaa tgaaaaagat
     2341 ggggctccaa tttcagatac tgtagaattt gaatttacgg gtgtagattc aagtaaatta
     2401 atcgcgaaag atgaattaaa aatccaaatc gtttctccag gttttgataa accagaagaa
     2461 ggttcaaccg ttattaagga atcaccaaaa gcggttgata aacaaaccgt ggtagttgga
     2521 tttaaaccag atgctaaaga atcaattcgg aataataaaa acttacctga agatgcagag
     2581 tattcatgga aaacagagcc tgatacttct aacgttactg atagtacgaa aggtattgta
     2641 actgttaaga tcggaaatcg aactttcgac gtggatgtag agtttgctgt aaaagcttct
     2701 caagctatgg aaaatgatgc aacatacgta cctataacaa caactccaga aacgacagtt
     2761 caaagtggaa aacctacatt tgataaacca gatgttcctt tagctaaaga tgccttttca
     2821 atttcagatg tctataataa gtacttcggt aatgcaagtg ttgatgcaaa cactggtatt
     2881 gttacattca ctccagctaa aggtgtagga gaatcggagc cgattactgg aacaattcct
     2941 attaaaattg tttaccaaga tggttctgta ggcacgaccg atttagcagt aactgtaagt
     3001 aaagatattt atgaaaatcc aggagaaaac attcctgcag gctaccacaa agtaaccttc
     3061 accgcagggg aaggaacaag tattgaaagt ggaacaacag tctttgcagt gaaagacggc
     3121 gtaagcttgc cagaagataa acttccagtg ttgaaagcaa aagatggtta tacagatgcg
     3181 aaatggccag aagaagcaac tcaaccaatt acagcagatg atacagaatt tgtatcaagt
     3241 gcaacaaaac tggatgatat cattgaaaat ccaggagaaa acattcctgc aggctaccac
     3301 aaagtaacct tcaccgcagg agaaggaaca agtattgaaa gtggaacaac agtctttgca
     3361 gtgaaagacg gcgtaagctt gccagaagat aaacttccag tgttgaaagc aaaagatggt
     3421 tatacagatg cgaaatggcc agaagaagca actcaaccaa ttacagcaga tgatacagaa
     3481 tttgtatcaa gtgcaacaaa attggatgat atcattgaaa acccagggga taacattccg
     3541 gctggctatc ataaagtaac ctttactgca ggagaaggaa caagtattga aagtggaaca
     3601 acagtctttg cagtgaaaga cggcgtaagc ttaccagaag ataaacttcc agtgttgaaa
     3661 gcaaaagatg gttatacaga tgcgaaatgg ccagaagaag caactcaacc aattacagca
     3721 gatgatacag aatttgtatc aagtgcaaca aaactggatg atatcattga aaatccagga
     3781 gaaaacattc ctgcaggcta ccacaaagta accttcaccg caggagaagg aacaagtatt
     3841 gaaagtggaa caacagtctt tgcagtgaaa gacggcgtaa gcttaccaga agataaactt
     3901 ccagtgttga aagcaaaaga tggttataca gatgcgaaat ggccagagga agcaactcaa
     3961 ccaattacag cagatgatac agaatttgta tcaagtgcaa caaaactgga tgataaatct
     4021 gacgctgaca aatataatcc tgaaggtcaa aaagtgacta cagaattgaa taaggaacca
     4081 gacgcatttg agggaattaa gaacaaagaa gatttaccaa aagatactaa gtatacttgg
     4141 aaagaaaaag tagatgttag cgcagctgga aataaaaaag ggactgttgt agtgacatat
     4201 ccagatggat catctgatga agttgaagta gatgttacag tgacagacaa tcgttctgac
     4261 gccgataaat atgagccaac agtagaagg
//
LOCUS       DACCXO010000157         4120 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.164, whole genome shotgun sequence.
ACCESSION   DACCXO010000157 DACCXO010000000
VERSION     DACCXO010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.164"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            572..2065
                     /locus_tag="IVY70_002679"
     CDS             572..2065
                     /locus_tag="IVY70_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP7959768.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            2200..2496
                     /locus_tag="IVY70_002680"
     CDS             2200..2496
                     /locus_tag="IVY70_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP7959769.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            2597..3448
                     /locus_tag="IVY70_002681"
     CDS             2597..3448
                     /locus_tag="IVY70_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959770.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            3469..3681
                     /locus_tag="IVY70_002682"
     CDS             3469..3681
                     /locus_tag="IVY70_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959771.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         1470 a          593 c          826 g         1231 t
ORIGIN      
        1 ttcatatact taatatattc gatatatcta atatactcca aattgataag aattcagact
       61 ttataaggaa tatagtggat atactaaata tatttgctat attcagtata tcagatatat
      121 tcaaaatgtc aaaatatatt tgtgcgtttt gcgttgacaa tttagagtga aaagaggtaa
      181 acttaattct atagaaacca cagataattc actatttaca aagtagcaaa agcagtggta
      241 ctctttaaac agaaaaatta cataaaaaaa agagacggaa ataattgatt ttagcagaat
      301 caattatttc tgaatcacat ggttaggaca tgtgttcacg ctcgtctcaa gaatatgtaa
      361 gtagctacca actacttata ttaaaagttg atcggatacc ttttccgatt gcctttattc
      421 taccaaaaaa aggcgagcaa ctcaacagaa gaacgtttat ttagtgtact taaattaaat
      481 attcttaaac actgaagaga gaacacatac tatggctgag attaaagcta aactgtgttc
      541 tctttttgtt tgagaaagga cgttacaatc gatgttgagc gaaatttatt cagctattct
      601 taaaaacggc ttacgtacat acaaatatcg aaacagcacc atgaaacctg caggtcatat
      661 tgattccagt aggagtggtg ctatctttgc gtaccgtagc aagcaattaa tgaatgttgg
      721 gcgtggtatg gtcattactt cggaagaagc tattttagag aacgaaaaga aactaacgca
      781 ttggacacct aatacctatc gctttggtac ttatgcggat gattatcgaa cagtggttaa
      841 aggtcattct gagaaaaact taagtcaaat caacacattt gtagttgata tcgattcaaa
      901 agagaaccat caaggcgaaa ttattttagc ctgtttagat caagtgggct acatgccaac
      961 gctaatttta gaatcagatc atggttatca agtttatttt gtactaaaga caccaggcta
     1021 tgtcacaaag aaaagcaatt tcaaggtgat cgatgtagca aaaaaaattt caaaaacaat
     1081 tcgtctacag ttggctgaaa aattgtccgg agtggatcta ggatgtaacc acttcggaat
     1141 tgctcgattc ccacaagaaa ataatattgt ttttaaagaa atgaactatc agtattcttt
     1201 tgctgactgg attcaatggt cgatgaagct tgaatcagat caaaaaacaa aagaagatcg
     1261 agaacgaaaa ctaattgtct ttcctgagaa aaaagaaatt cgtcaagtag atgaaccgtg
     1321 gttcgatatg ttacttcata aagcaaacat cgtcggcgga gaaggacgtc ttggtcgaaa
     1381 taatgtgatt ttcacgcttt ctttagctta ttactcttca ggttatgcat tggatacttg
     1441 cgaatacaat atgttcacat tcaacgaacg gttacaggaa ccgctatctg aagcagaatt
     1501 agtaaagatt gtacgtagtg cttattctgg taattatcaa gcagcaagca gagaattcat
     1561 tattgaattg tgccagcaat gggtttcacc aaccttaaaa aataatgatc tgttcattca
     1621 acgacgtggc tggtggaaat ttaaaaagcc acgagatcaa agaaaatata gtcataaaaa
     1681 tgaatggaaa gaagatctat tggcttatct aagcgaaaaa agtcttgcta gtgagcctta
     1741 tttgaacctg tcaaaaaaag aattggcttc acaactaacg atgcccaagc gaacacttga
     1801 taagatctta aatgagttac gagaggagaa taaagtcttc tacaaggtaa cacgaggaag
     1861 aaatggcgga atgatcattg cttcagtcaa aacgttgttc gcgagggtta ttcaactaag
     1921 taaagaaaag agactagatt attcaacaag gatcacagag gtatttggac tcaacgagag
     1981 cttagtagaa gagacattta gacaattatc aacccccttg aaccctctta aagaagtgaa
     2041 tctttttgaa gtagatacag gttgaaaaat aaaaggtgca aattgcctta tacaatatat
     2101 ctatgatact tctgtaggtg ttgctccctt tgcctagcta cgtgtaggtg gtatacttat
     2161 aactgcgtat gcagtcttat gtatcatgga ggaagttgta tgtcgttaaa aaattatgtc
     2221 tattcacatc ctgttaatgt gtttatcatt ggtcgtttgg gattatctgt ggaagtgttc
     2281 tgtgagcttt atggttttaa gcaggggact ttgtcttctt gggttaccag ggaaaaaaca
     2341 gttgcttctt tgccgattga gtttcttcat gggttgtcct tagcttctgg ggagactatg
     2401 gatcaggtat atgattgttt gtgtgtgtta gagcaggagt atattgagta taaaatagct
     2461 aacgagttaa gaaaaaggaa gaagtatatc cagtagtagt ttttatttac ttaaaaaatt
     2521 ggttcattga gcaacctatc gctgctaaag tagcaaagga aaacgttaaa ggaaagaatt
     2581 tagggaagaa atcgaaatga aatcggacga ctggattgaa cacacctggc ttcctctgaa
     2641 aagaaatagc aaacgaaaga aattttgggt attgcaatta tggccattag gaaaactttg
     2701 tagttacggg gcacgactta ttcttgattt aaccgtttac tcgattggag tatttggaac
     2761 ggcttattac ttgataaatg atcccaatag gattgcagca tcagacattt ttacagtgct
     2821 aatgacgttg atttttgtca tgctattgtc tatttttact caattagtca tgcaatttac
     2881 caggcaaaaa ttaacagcag gaaaactttt caaaagccta ttcgatttaa ttgttgtaaa
     2941 tgcaattcta gcgactttaa taaaacaaat tgaacaatct ggaagaacaa caaaaggagt
     3001 gaaggatttt ttattgacgt ttattcactt tgtaaatgga cacatgtatt tgtttgaagt
     3061 gggtattatt ctcttttttg tcgggtatgt atccacgaaa cttattaaac gttcaattga
     3121 aaaagaaatt gaaattgacc tgattccgac acttcttggt tatgagtaca atgttgattt
     3181 tggaagatat gacgaattga tcaatacgga tatcgttcac acgagtatcc atgaatctac
     3241 tgttaagggc atcagatcta ttttaaaaaa tggccaggaa atgaaagcaa tagatcgaag
     3301 aaaagagatc tacaattttt atgaagtcga agcaagccta cgatatggca tcggaaaatt
     3361 gtggttagat ggaagtaata ttcgtcaaaa caaaccatac attttttgga acagtagtga
     3421 tttagaactt ccaatgaaag ataaataatt atggagaatc gtttgaaaat gattcctcta
     3481 tacaggattg tgcttatagt tggcatggag ttaaaaacct taaagaagga gcagttattc
     3541 aaagtgaaag aacgtcataa tcagttacta tgtagtaaaa cgggtacgat gaacattgaa
     3601 taccgaacag aatatttaga attaacgaat caaactgatt ttttgttgat tcgaaagcaa
     3661 caagagcaag aaaaaaatta atggctactt atagccaatt gattgatgaa tgggaacaat
     3721 tcaaggaatc taaaattcca gaacgtctct aaaagccttt atttgtaaag agcataagga
     3781 aacgtgggaa cactttttaa aaaaagtgtt ccaataggct gtgcctatta cgtttctcaa
     3841 aaataaatgt gggaacaaaa tatttaaaaa taggatagtg aaaggatagt gaaggatagt
     3901 aaaaggaggg tagtaggata gcaaaaagga taggctcctt ttcgctatcc tttttaaatt
     3961 ggaggaatta atatggaagt aagaataaga aatgtggatg agaaaatcgt aagagaaatt
     4021 gatcgaagat gtgacgaacg gttctgttgc aaagttttaa atctactatc aaataaggta
     4081 gaataataga aaaagatagc aggaggaatg acgatgaatc
//
LOCUS       DACCXO010000158         4073 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.165, whole genome shotgun sequence.
ACCESSION   DACCXO010000158 DACCXO010000000
VERSION     DACCXO010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.165"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(182..1687)
                     /locus_tag="IVY70_002683"
     CDS             complement(182..1687)
                     /locus_tag="IVY70_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7959772.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1684..2370)
                     /locus_tag="IVY70_002684"
     CDS             complement(1684..2370)
                     /locus_tag="IVY70_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7959773.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2563..3984)
                     /gene="gndA"
                     /locus_tag="IVY70_002685"
     CDS             complement(2563..3984)
                     /gene="gndA"
                     /locus_tag="IVY70_002685"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP7959774.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1121 a          859 c          694 g         1399 t
ORIGIN      
        1 atattacagc attttattgt tttttcgttc atctaaaaga tacttacatt taattaacgg
       61 atgttattct atttaattgt tcgctagttt ttttataatg acatccctga atttctcact
      121 tgcttgtatt ttgtacctta ttctttgttg gatatataaa aaaacaagta aagcacatgc
      181 tttacttgtc ttctgccttt gcgataggaa taaagattct aaaaattgtc ccttgcccta
      241 ctacactttc tgctaagata cgtcctttat agttttcaac cagttgttta gcgatagaca
      301 accccagacc atttccaccc ttcgttctag ctcttgcttt atccactcga taaaaacgat
      361 tgaagatctt attaaggtct tctttgggta ttccttcgcc aaagtcctga atcgcgatct
      421 caaattcatt cagagtggaa gaaatagaga tatggacttc ttttcgtgta gttgaatact
      481 ttactgcgtt atccaatatg ataatgatta attgttcaaa gtgattacga taaatttgca
      541 gggtcacttc ttttgtcaaa tcatcatcta aagtgatcac atagtctgga tataatatct
      601 tgaaattatt ataaacttga taggtgactt cttttccttt agttgtttca ttcgcataat
      661 gaacatctac ttgttctgct cttgataagt ctagcatctc ttgaactaag cttttcatac
      721 ggctgatttc ttgcatgctg gcgcttaatg attcttctaa tatctcagga tcatctttcc
      781 cccaacgatt tagcagacta agatgccctt caataattgc aacaggtgtc cttaactcat
      841 gagatacgtc ttccacgaat tgctcttgtt gttcaatata caaacgcata cggtcaagca
      901 tctcattgaa tatttcagct aaatctgcca attcatcatt tgtgtcgatt tcaggcatgt
      961 ggatatctga ttgcggatct ttacgtatag tatccattgt atctctgagt accttgagtg
     1021 gttttaagaa ataagaagat aaaaagaaac ctaaaatact acttaagatc aaagaaacaa
     1081 cttctaaaac aataagagtc aataatagct tatttcgaat atcatagaaa gaagataatt
     1141 catagaagct ttgcgcatag ccgatctttt ctcttgtacc tttagaatat ataggctgga
     1201 tagataaaaa gccagttttt ccttctagtg taacgatcgt tggaacttta cgtgtagtct
     1261 gaatcaggtt tcgactgttt tcatgggttt taaaaatcaa gttctcgtcc aaatcataaa
     1321 cataaagatc cattgatggc tgccctagct cagctataaa ggaatcgatc ttcattaagt
     1381 ttccttcgac cagtgaagta tgatctacga cctcatctcc gtcatttgtc gctttcgtca
     1441 gattacgata cgtatttact aatgtcagtt cttcatccga gttagccaaa cgagaagcta
     1501 cttctgaaat cgtacgctct acattgtttc tttcttttgc cacaatcaga ttgatagatg
     1561 atttataggt aatgacagcg aaaattgtaa acacaacgaa tatgaagaaa gaactcgcaa
     1621 aggcccactt gattgtcaag gagggccctt tcagttctct tttttccgat tttttcggct
     1681 ttatcacgag cgcatcacat aacctgttcc gcgtacagtt tggatatagc tttcctcccc
     1741 gggaacgtca attttgttgc gaagataacg aatatatacg tccactacgt ttgtttcaac
     1801 ttctgtttcg tatccccaca ctttatttag taaaacgtca cgtgctaata ccacgttcac
     1861 gttttccatt aaagtaagca gcaactcgta ttctcttttt gttagttcaa ttacttcatt
     1921 tccgcgacgt acgacacggt tttctttttc gatagtcaaa tcgcgataag taatagtagt
     1981 ttgtttagcc acatttttat cgccttcaat atcgattcga cgcaacaatg cacgaagacg
     2041 tgctaataat tcttcgatag caaatggttt gacgatgtag tcatctgctc cgtggtcaag
     2101 tccagataca cgatcgatca cagaatctct tgcagtcatc ataatgattg gtgtattttt
     2161 gacttggcgt acacgtctgc agacttctaa accgttcaat tctgggagca tcagatccaa
     2221 caatatggca tcccactcat tatttaatgc cgcatctaaa ccagttcggc cgttgtagtg
     2281 gacttctgcg ttataccctt catgcttcaa ctctagctcg acaaatcgtg ccaagttctt
     2341 ttcatcttca atgattagaa tattgctcat ttattatttt atcctttcta ttctactggc
     2401 tttttaaaaa ctctatcatt ataccgataa tatgcttaaa aatctatagt cattttatca
     2461 tggatagcaa ttttaaaaaa gagctgaagt ctactcataa caatcgactt cagctctttc
     2521 gtcctctttt tattttctca tttcctaaca gccgaaagac tattattctt catggtacca
     2581 tgagtaatgg aagattcctt ctttgtcgac acgctcatag gtatgtgcgc caaagtaatc
     2641 gcgttgggct tggattatat ttgcaggcag acgttctgct ctgtatgaat cgaagtaagc
     2701 gatggctgat gagaaagtcg gtacaggtac gccagcttgg acagccaagg caacgacgtc
     2761 tctgactgat tgctgatatt ttttcgtgat ttcaacgaag taatcatcta gcaataaatt
     2821 ttcgatctca ggatttttgt cataggcatc cgtaattttt tgcaagaatc gagctcggat
     2881 gatacatcca gcgcgccaga tttttgcgat ttcaccaaat ggcaagtccc aatcaaattc
     2941 ttttgaagcg acacgtaatt gagcaaatcc ttgggcataa ctcatgattt tactgaaata
     3001 caacgcttct cggatttttt cgaccaattc ttttttgtct cccgtgaact caaaatcgtt
     3061 cgtacgagac aaaatcttgc ttgcttgtac gcgttcttct ttataagcag aaatatagcg
     3121 ggcaaataca gattcagtga tcaatggaag tggcacacct aagtcaagcg cgctttggct
     3181 tgtccattta cctgttcctt tattcccagc tgcatcaagg atcacatcta ctactggttt
     3241 acctgttcct tcatcgtctt tacgtgtcag aatgtcagca gtgatttcta tcaaatagct
     3301 gtctaattcg ccttgattcc attctttaaa gatatcagcc atttcttcta ctgaaaggtt
     3361 taaaatattc ttcattaagt cataagattc agcaatcagt tgcatatctc catactcaat
     3421 accgttgtgt accattttca cataatgtcc ggctccattt gggccaatat atgtgacaca
     3481 tggtgctccg tcttctgctt ttgcagaaat ttgctctaag attggtgcaa ctagcgcata
     3541 ggcttctttt tgtccaccag gcatgattga aggtcctttt aaagctcctt cttcaccacc
     3601 ggaaacgcct gtaccgataa agttgatgcc agagttggct aactcttcat tacgacgcat
     3661 cgtatcttta aagaatgtat ttcctccgtc aatcaaaata tcgcctttat caaggtgagg
     3721 aagtaactct tggattgttg catctgttgc cggtcctgct ttgaccatta gcaagatccg
     3781 acgtggtttc tcgatagatt ctacaaattc ttcgattgta tatgttgctt tgaaattttt
     3841 atctgggtgt tcttctacta cagcggttgt tttagcccct gtacgattga ataaagccac
     3901 tgaatagccg cggctttcta tgttcaaggc tagattttta cccataacgg ccataccgac
     3961 aacgccgatt tgttgttttg acatatgaac tcctcctaca tacgggattt ctccctaatc
     4021 tcttacatta tagcttaaca cggctacatt gcaaactaaa gaacaacata ttt
//
LOCUS       DACCXO010000159         3878 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.166, whole genome shotgun sequence.
ACCESSION   DACCXO010000159 DACCXO010000000
VERSION     DACCXO010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.166"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            955..1878
                     /locus_tag="IVY70_002686"
     CDS             955..1878
                     /locus_tag="IVY70_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP7959775.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            1894..2433
                     /locus_tag="IVY70_002687"
     CDS             1894..2433
                     /locus_tag="IVY70_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP7959776.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1452 a          596 c          653 g         1177 t
ORIGIN      
        1 aggtatttgg ctagcaaata tagagcttgc caaacccttt tagtgagttt tgtagaaagt
       61 acctttaaag gtactttcac attacgaacg atacgtccgt aggacgtact gaaagggtgg
      121 gacttatgag caaattaatg caaaatttgg ctagcaaaaa agctaaaaaa tatatgctaa
      181 taatcgagcg aaataaacac tttttcaagt gagcgaatcc gccatgacaa agctgaagca
      241 gtttatttca gctttgtcat ggcgtgtgcc gactttttta aaagtctagg cacacgagca
      301 aatatcttta tcaagctcta atttgcgttt taagacgttt gaaacgccta agtatataaa
      361 tatataagaa taggtttaag agtgcttaaa tcgaatttta gagggcattt agacaatttt
      421 agggttgtta gggcttgccc tgaccgcctg taaggcgttt taagaacaag ataagggatt
      481 tatactagga ctgtcttaaa atgtcttaaa cgaacgctca gagcttctca aagggttcct
      541 tatgaacggc ttttagcaaa ggagccggag gcgactgcgc agtataatgt gagagcagag
      601 cgatcacacc tttttagcga accgtaggga gcaaggtttg gaattggcag ttttgataaa
      661 ttcgcgaacc tgtttagaga aaaaaggagt gattttttag gcgggaaaat tagcctcccc
      721 tctttttata tctcttttta tatctctttt tatatctctt ttatgtcggc tgtgtcctta
      781 ctctcccaag caattcagag ttttttcaag taggttttct acgcttttca agtaggtttt
      841 ctacgctttt caagtaggtt ttctacgctt ttcaagtagg ttttccgaca ttattgagta
      901 tttttataaa gtatgttact tttatttcat actttataaa agtaggagaa gcttatggca
      961 aatgaaattg taaaatatga aaatagatta aatcatattc ctctgagaaa atttaatagt
     1021 cgtgaaatga acttattttt ttcaattgct tcacgtgtac gtgaaaaagg tgtagatgag
     1081 attatttttt catttcacga tttgaaaaaa ctaagcggtt atacagaacg tggggaacaa
     1141 tttataaaag atcttagtag cacgtatgac aaattattaa gtattaatgc ttggaatgat
     1201 gatggaaata ctttaactaa atttgttgct ttcacaaaat atagcattat acgagataaa
     1261 caacttgtta taatttcagt taatccagaa tttaaaggac tttttaatca attaaagaac
     1321 tggacaagat ttaatctaga gcaatttact aatttggata gcacatattc aaaaactatg
     1381 tttcgattat taaaacaata tcgaactcaa ggctggactg aattttccaa agaaaaattt
     1441 tttgatttac tagatattcc taaaagttat tggaatagtc cgtcaaatat tgacaaattt
     1501 atcttaaggc caattaaaga agaactcact cccttattta aaggattaac tattcgtaaa
     1561 aaatatggta aaggtcgagg taaacctgtt atcggttatc gttttacttg gaaagctgaa
     1621 attaaccatg ctgacgattt ttccaaaggg aaacaagaag atttacgtat aaaattattt
     1681 aatattgatc acaatggaga attaacacga gaagaaaaat ggcgcgctaa agatcgaata
     1741 ctaaatcttc ccttaggcac tcatgaagct gattttaaac aacaacaaca aactgaagaa
     1801 aaacaggcag aaaaacgaga actttcaaac aaactcaaac aagatttact aggaaattta
     1861 caaaacttat tcgattaagc ttaaggagta tttatggcag ataaaacaat caaagaactt
     1921 gcggaagaat taggagttag taaaacagca attaacaaaa aagtttctga caaaaatcga
     1981 aaactttggt ttgcaaaaaa cggaaacaag tttgtgatta acgaaaccgg tcaaaaagct
     2041 ataaaatcaa tgtttttagt cgataacgca aacttaaatc gaaaactagt tggcgaaaaa
     2101 tcaaaaacca aaaaccataa aaacaccaac caagtttccg aattaaaact cctttctttc
     2161 ctagaaaaac agttaaatcg aaaagatata cagctacaag aaaaagataa tcaaataaaa
     2221 caaatgcaaa aattactcga ccagcaacaa caactcactc tacaagcgaa ccaacaaatt
     2281 gagaacctac aagaacagct ccatttaact tatacagaag aaactccaac aaccgaatca
     2341 actgcctttt ctgaaaaaaa agcagacaat acacaaccac ttattgaaaa aaagtggtgg
     2401 caaatttgga aatctaatag aaccaatctc taaaattttc tagaagttag ttcggatagt
     2461 agaggaaaaa gttgtactac ctgatgggga gatcactcaa atttttatga gaaagaaaaa
     2521 atagtttaca actgtaaaca atcaatgttt atagttgtaa actatcaaac tatatggtga
     2581 cttattgctt aaaataggtt gaaatctcac tcaatttttg gtggaaaaac aaaataaatt
     2641 ctaatcgatt ttgttgaaaa agtcataaaa aaacaaatgt gattttaaca aaatttcaaa
     2701 aaaatcgttt gaaatttcgt ttattttttt tgagaatttt gatcatcgta gggtcattgt
     2761 agaatcatta tgagatcatc gtgggatcat cgaataaagt atgggaaagg cttgcggtgc
     2821 ttggtattaa gggcattctc cgcctcccag gttcttgacc attttctctc aacgtggtaa
     2881 aaatcccact tataagagaa aatggtcaag aacctaaacg gtcggcgaag aaaacatacc
     2941 aagcaccaca agcaaaaaaa gttagagtaa cttccaaaaa agaaaaattt tttagaacaa
     3001 cttttgaaaa tttaattttg atttatatcc gttcaattta aacatttgat aaactgaagg
     3061 ctagtattta aaatgtgtat acaagtggta gcgttaaaaa aagtggaggt ataacctatg
     3121 ttaaaaaaaa ctgtgttggt actgggttct atttctctag gaatattagg ttttagcccc
     3181 atgctattat ccgtaattgg aaaaaacaat tccaacggag aacttaaatt aaattcaaat
     3241 tattacaaca gtcctaacga agaattgttt gtaaaagaga cggataaata ataaaaaaga
     3301 ctgctctttc gagcagccag ttaaaaaata atgagtgttt attcttgaaa aagcaacgcg
     3361 cttatgaagc gctaccaaaa atttatcata ttttatgaaa aataacaccc cagtaaaaaa
     3421 tgactggtaa aaattcacaa agaaacggaa agaagcaact gcagaaaatc aagccttaaa
     3481 aagagctttt gacttagacc aatcgaaaat cacaaagtga gcccctgtat cccttggtag
     3541 agaggctact tggcatagta cacattatgt caacaagaat ttaatataac atatttttct
     3601 agtttgttat aacaatgcca ctctaacaaa aatgtaagca ttatccttat taaaagctcc
     3661 ttgacaagac tatacctttt attttatgat ttttacatag aaaacgtttt ctctattttt
     3721 tgtttagagg tgatttttat gatttgcaag aacaagaaaa aaattattgc tattattgct
     3781 agtttctctt tgatttttag tactgcatgt actgtcttat taacaaataa tgctaatgtt
     3841 gaagctgcaa gctcttctta caaacataat catgctgg
//
LOCUS       DACCXO010000160         3839 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.167, whole genome shotgun sequence.
ACCESSION   DACCXO010000160 DACCXO010000000
VERSION     DACCXO010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.167"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..399)
                     /locus_tag="IVY70_002688"
     CDS             complement(<1..399)
                     /locus_tag="IVY70_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7959777.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNF"
     gene            687..1961
                     /locus_tag="IVY70_002689"
     CDS             687..1961
                     /locus_tag="IVY70_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7959778.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            2016..3581
                     /gene="guaA"
                     /locus_tag="IVY70_002690"
     CDS             2016..3581
                     /gene="guaA"
                     /locus_tag="IVY70_002690"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP7959779.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
BASE COUNT         1210 a          775 c          713 g         1141 t
ORIGIN      
        1 aaaattaacc gatagatcct tatattcaaa cttctctaaa tgtccataat tcttcttaat
       61 taaagaatct attctagctt ttaagaccgg caatgaaaat ggcttttcta catatccgtc
      121 tactaaatta gtaaatgcat caattttata ttcttcatca ctaaatgcag tcaagattag
      181 aattggtaaa ttgcttttct ttctaatttc tttcaacact tctaaaccat ttataaaagg
      241 tatctgaatg tctaagataa ccaaatttat atcgctatta aattttgaca aggcttctct
      301 tccatcttta gcttgaataa cagtatatcc aaattctgaa agatattcac ttattccctc
      361 tcttatcata ttatcatctt caacagttaa tattttcata tatacctcca cttaagccta
      421 tacactagat attatacagc atattatggc tctataatat ctagtgttgg tgacaccttc
      481 tgttgccaat actttttctt ttttctaata aatgcaaaca gacaccttga aatcctttag
      541 aataaagggt tctataatat tttgtgttgt tggaaaatcg aaggcgattt tctactaaca
      601 ctttttcttc gtttcaactc aaaaagagac tcacttttgg taaaatttag tcgaccaaaa
      661 catcacccta ccaaaggagt cgctctatgg ataatcatac tagaaaatta ctcggattaa
      721 cagataaaca tttatttttt gatgaagaat ggctgattga aaaagaatat aaaggcgttc
      781 aagcgcagtt tattaaaggg agattggatg actcaccttc ccattgtgaa cactgtggat
      841 cgattcgtat tatccgcaac ggttcctata cgacacgtac tcagatattg aaagtcaaag
      901 aaaagttgac tattttagaa ttaaaacgta cccgttttct atgctatgat tgtgggcaga
      961 ccttttctgc gaaaacggac ttagttgatg aacatcatca actaacaaag gaactgaagc
     1021 aggcgattct catagaactg tacgaaaatc aatcacgtaa actaatcgct aagaagtact
     1081 tcgtctcgga tgggacggtg acacggatct tgcgtgaagc aacgaagcat taccagccac
     1141 gtatgaactt ccttccgacg gtgttgtgta tggatgagtt caaatcaatg aagtctgttt
     1201 ctggttccat gagttttatt tgtgttgatg ggacaaccca tcagctattt acgatccttg
     1261 aagatcgcag actttacaaa ctaactcaat acttcctgcg ctttcctaga aaagcccgtt
     1321 taaaggtcaa gtacctagtg atggacatga atgccagcta ttgtcagttg cttaaaacag
     1381 tctttccaaa tgcggagatc gtaactgacc gctttcatat tgtccaacac atcaatcgca
     1441 gcttcaatca attacgtgtc cagatcatga atcgttttcg aacctcacac tctgaaaatc
     1501 agaagaagta ccgtcggctc aagcgatatt ggaaactctt actcaaagat tccacgacat
     1561 tggagccact caaacggcat tatcatcgac tgtttaaacg accaattagt caaacagaaa
     1621 tcgttgatga gctgttaagc tacaacgaag aattgcgaac tgcgtatcat ttttgtcaat
     1681 tattacgcta ttatttcgtt aaacgaggta cggaaggctt tcaagaaacc cttaaaacaa
     1741 tttcatctac gttacctcag tcgtttaaaa agaaattcac gatcttccat cgctaccaat
     1801 cgggtatttc aaatgccttt aaggtgtccc actcaaacgg cgtcactgag gggctgaata
     1861 acaagatcaa gctgattaaa cgaattgcgt ttggttatcg caacttttat aactttcggg
     1921 cacggatcta tttacaacaa ggtctgatct ttgaaaacta aaaaaatatg cggcgcacag
     1981 aacgcgccgc atatcggact aagttttatt caaatttgtc ttcaccaaca cgatttgacg
     2041 aagagccaaa aattatcgta cttgattacg gtagtcagta taaccaactg atcactcgcc
     2101 gtattcgtga gttcggtgtt ttctcagagt tgatgagcca ccgcattacc gcagaagaag
     2161 taaaggcaat gaaccctaaa ggaatcatcc tttctggcgg accaaacagt gtttatgatg
     2221 agaaggcctt tggtattgat gaaaagattt ttgatttggg gatcccagtt ttaggaatct
     2281 gttacggtat gcagttgatt acttataaat taggcggaaa agttaaacca gccaaaaacc
     2341 gtgaatacgg aaaagcagaa ttggaaatca cttctgcaga agcggccctt ttccaaaaca
     2401 ctcctgaaaa acaaaccgtt tggatgagtc atggtgacct agttacccaa gcaccgactg
     2461 atttcgatgt cgttgctacc agcaaagatt gccccatcgc atcgatccaa aatgcagaac
     2521 gcaaaatgta cggaatccaa ttccatgcag aagttcgcca ttccgaatat ggaaatgact
     2581 tgttgcgcca ttttacacta gatgtctgcc aatgtactgg tgactggaca atggataact
     2641 tcatcgacat ggagatcgca aaaattcgtg aacaagtcgg tgacaaaaaa gttttattag
     2701 gcctttcagg cggagtagat tcctctgttg ttggtgttct tttacaaaaa gcgatcggcg
     2761 atcaattaac atgtatcttc gttgatcacg tattgttgcg taaaggtgaa gcagaacaag
     2821 ttatggaaag tcttggcgga aaatttggct tgaacattat aaaagtcgat gccaaagaac
     2881 gtttcttatc aaaacttgct ggcgtgtctg atccagaaga aaaacgtaaa atcattggaa
     2941 atgaatttgt atatgtcttc gatgatgaag caaccaaact tgccggtgaa gaaggcgttt
     3001 ctttccttgc acaaggaacc ctttataccg atgtgatcga atcaggaact gaaactgccc
     3061 aaaccatcaa atcacatcac aatgtaggtg gattacctga agacatgcaa ttcaagctga
     3121 tcgagccatt gaacacattg ttcaaagacg aagtacgtga attaggtacc caattaggca
     3181 tgcctgacag catcgtttgg cgccaaccat tccctggacc aggcttaggt attcgtgtcc
     3241 ttggtgaatt gacagaagaa aaattagaaa tcgtccgtga atcagacgct atcttgcgcg
     3301 aagaaatcgc caatgccggt ttagatcgcg acatctggca atacttcacc gtcctaccag
     3361 gcattcgctc tgttggggtt atgggagatg gccgtacgta tgactacact gtaggtatcc
     3421 gtgctgtcac ttctattgac ggcatgaccg ctgactttgc ccgtatccct tgggaagtct
     3481 tgcaaaaaat cagtgtccgt atcgtaaatg aagtcgcaca cgttaaccgt atcgtgtatg
     3541 atattaccag caaaccacct gcaacagtgg agtgggaata aagagccaac ataatatgga
     3601 tgaggaatac ctataaatca acattattca attactaaag ttcctgattt tcgtaaaaaa
     3661 taagatggtt tcctaccatt ttccctacca aagcccttgt tagattaact actaacaagg
     3721 gctttgcttt tattcactac ctttattttc ctattgaatt agctttttat ctaatcagat
     3781 ttttagtact ataaatatat atatcagatt tttcgattta aaagatctgg taaaaagaa
//
LOCUS       DACCXO010000161         3805 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.168, whole genome shotgun sequence.
ACCESSION   DACCXO010000161 DACCXO010000000
VERSION     DACCXO010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.168"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            70..1038
                     /locus_tag="IVY70_002691"
     CDS             70..1038
                     /locus_tag="IVY70_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7959780.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            1049..1864
                     /locus_tag="IVY70_002692"
     CDS             1049..1864
                     /locus_tag="IVY70_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAP7959781.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKKIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            1932..2642
                     /locus_tag="IVY70_002693"
     CDS             1932..2642
                     /locus_tag="IVY70_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7959782.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            2678..>3805
                     /locus_tag="IVY70_002694"
     CDS             2678..>3805
                     /locus_tag="IVY70_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP7959783.1"
                     /translation="MKQVLRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1260 a          559 c          848 g         1138 t
ORIGIN      
        1 ttacatttaa tcagttactt ttattttgga taaaattaac atttttaaaa ttttaaggag
       61 gaaatgttga tggatgtagc aaaaacggtt aacgcaattg tagaaaaaaa tgatggagag
      121 atttctggaa tttatttggt ggcttgtggc ggttcgctgg tagatatgta tgtctcagat
      181 tattttctta aagccgaagc atcgaagtgt attacaggct tatatacagc gaacgaattt
      241 aaccatgtaa ccccaaagca attagatgat aaatctgttg tcattctatg ttctcatgga
      301 ggaaatacag cagaaacggt aaaagcagga gaagtcgctc aagctagagg agctgtgact
      361 gttggtctga ctcataataa aactgcggat ttattaaagg tttctgatca tagtttcctt
      421 tacgaatggg gagatgatgc aaaaatagtc aataatccta tggccatcat tcttgattta
      481 actgtatctt tggtcaatgt taccgaaaca tttgaacatt acggtaaatt cagagaaggc
      541 atgaaggtta tcgataccgt aattgaaaat ggcattaagc aggtgcagga tcgtattaaa
      601 gtgtttgctg ataagtacaa agatgaaaca atgttttata tcttaggtag tggaccatca
      661 tatggacatg cttatggttt ttcaatttgc tctttgatgg aaatgcaatg gcttgattca
      721 acttctgttc attctggaga attcttccat ggaccttttg aagtaacaga caaagatacg
      781 aattttattc tcttgatgag cattggacgc actcgtgctt tagatgaacg ggcaaaacga
      841 ttccttgata aatatgcaga aagagtagaa atcgttgatg caaaggaact aggattggat
      901 atgatatcag atgaagtttc tgaattcttc aatcccattt tattctacag tattctttca
      961 gaatatcgtt cagctttagc agatgcacgc aatcatgatt tagatgttcg tcgctatatg
     1021 gggaaagtgg agtactagga ggattaaaat gaaggtatta ggcttaggtg ataacgtcgt
     1081 cgataaatat gaaaatctgg gaattatgta tcctggagga aattcattga attttgcagt
     1141 ttttgcaaaa aaactgggac atgaaagtag ctttatggga gtttttggct cagatgagga
     1201 ggcagctcat gttttatcag caataaaaaa aataggactg gacaacagtc actctcgctt
     1261 tgaaaaaggc gagaatggct gtgccagagt gaagattgat gagggcgacc ggattttcct
     1321 tggcagcaat tcgggaggtg ttactcgtga atacccgatt cagcttactg aagaagacag
     1381 agaatattta aaccagtttg aattgattca tatgggatta tatagccatg tcaatcatct
     1441 attagaagag ttacaaaatg tttctggaaa gatttcttat gatttttctg acgactttac
     1501 ggaagaagag gttcagcggg caattgataa agttgatttt ggcttctttt ctatagaaga
     1561 cttttccgat gaagaagtaa agcagttttt gatgaagcat ttttgtgagc gaaatgatgt
     1621 attgattgca acccgaggag aaaaaacggc tattggttat gatggagcaa agttttatga
     1681 agtgaacccc gtgctagaag aaccagttga tacaatggca gcgggagatt ctttcttaac
     1741 agctttccta ctttattttc tcgaagggga cgatatagtc accgcgatga aaaaggggaa
     1801 tgaatttgct gggaagtctt gtctggttga cggttcgttc ggctttggta ttcattacga
     1861 ttgagcttgt tttaaattcc tgcatttgat aacctaaaga aagaaatccc ggtaaagtgg
     1921 aggttaaaaa aatgctggat acaaatgcac aattaccatt gtacttacaa ttaaaagaaa
     1981 tattaaaaaa taaaatcaat acaggggagt atcccgagaa tacgaaaatt cccgctgaat
     2041 cagagctgat agaaagttat gaggtcagcc gaatcacagt gagaaaagca gtagaagaac
     2101 tggtagaaga agggtaccta cttaaaaaac aagggaaagg aacttttgtc aaccggcata
     2161 aagtttttcg taaaattgaa tatgttatgg gtttcagtga gagttgtaaa tctaatggtt
     2221 ttacaccgac tagcattgtt ttggaacgca agatcattcc agcaactgaa gggatggcaa
     2281 aaaaactcga tatcgctcaa ggtgatgatg ttttatatat ccagagaaag cgaatggcgg
     2341 ataacatccc cattatgtta gaaaacaatt atttttctta tgaaagatat agtttcttaa
     2401 tggaggaaga gctgactgat tctttgtatg aagtacttaa agtccaaggt accttagcca
     2461 tcaatcctgg ggaaactgca cttgaaattg tcacagctga tgaccaaatt gccaaaataa
     2521 tggaagtagc tgttgggacg ccctttttct ttatggatac tttaatcaac gatcaaaaca
     2581 acataccaat tcatgtaggt aatcaatatt acctgggaga agtatatcgt ttttacttat
     2641 gatttcctgt tttgatttca aggagtagat tttatttatg aagcaagtat tgcggtcaaa
     2701 aatgattttt gatggagagc agccaccttt caaaggttat attgtaatca atgaagggat
     2761 cattgaaaag gtggaaaaca actggaatta cagtgatcta ttaaatgaaa acacggaatt
     2821 aattgcttat gatgatcaat tcgttatgcc agcaattcat gataatcatg tatttttcag
     2881 tggttatttg gctatgaatg ctggcataga tttaagcgat acatcttcag ctaacgaggc
     2941 tgtagagcgt attaaagctg taatgaacca taaaaaagca gatgaaccaa tttatgctca
     3001 tggctgggat gctgccctgt ggaaaactga cccatcagaa gagctattga atgaaataaa
     3061 ccactttggt ccaatcacag cgatcgacaa aaatcgcagc cattgttgga tgaatgctat
     3121 tgctaagcag acttatggtt ttacagaaaa agaaaccagt gctgaaagtc gagtattatt
     3181 aattaaggaa atgctagcaa acaaaaagct tttagcagag gtttacgctg aatttgaagc
     3241 attactgctt tcaaaaggtg ttatatcaat gaaagaaatc gtttttgatg acgcagaatt
     3301 tctttctaat atacctcaga ggaaaatact tagtaaccta tatgtgcagg cagtccaaga
     3361 acctctacag catgaacttc tatatgagta taaggaaaaa gttttccctg aaaaggtccg
     3421 ctttggtgga gtgaagatca tggtagatgg tgttgttgcg gatagaagtg gagatattta
     3481 cggtgtttat cagtcaggag tagcttcacc cgagattgac tatgaggcaa ttaaagcaga
     3541 agttgctgca ttcaatgaag tagagattcc ttgctgtttg acaacggaag gagataaagc
     3601 gggttataaa gctgcgtcaa ttcttgtcga atttggaaag cggttacctg aaggcgtttt
     3661 taattcgatt agtgatttgg aaatggttac tcaaaagaca gcagaaaaaa tgaacgaagg
     3721 acaagtagta gcagaggtat atccacaaat tctcggcttg aatcaaacgt tagaagaggc
     3781 ttacatgtca actgttataa aaaat
//
LOCUS       DACCXO010000162         3765 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.169, whole genome shotgun sequence.
ACCESSION   DACCXO010000162 DACCXO010000000
VERSION     DACCXO010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.169"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            155..499
                     /locus_tag="IVY70_002695"
     CDS             155..499
                     /locus_tag="IVY70_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP7959784.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            500..919
                     /locus_tag="IVY70_002696"
     CDS             500..919
                     /locus_tag="IVY70_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP7959785.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1011..1361)
                     /locus_tag="IVY70_002697"
     CDS             complement(1011..1361)
                     /locus_tag="IVY70_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959786.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1327..2658)
                     /locus_tag="IVY70_002698"
     CDS             complement(1327..2658)
                     /locus_tag="IVY70_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP7959787.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2913..3479
                     /locus_tag="IVY70_002699"
     CDS             2913..3479
                     /locus_tag="IVY70_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7959788.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3624..3711
                     /locus_tag="IVY70_002700"
     tRNA            3624..3711
                     /locus_tag="IVY70_002700"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3656..3658,aa:Ser,seq:cga)
BASE COUNT         1392 a          647 c          727 g          999 t
ORIGIN      
        1 aactatgctt aaaaagcata tcgactgaat aaactaagca ttggacaggc tgaaagacta
       61 catttaaaat gaaatgcgaa gaaacaatag aaaataaagt tgccttagaa ccatagttga
      121 atcacctaac agtcgtgaag ggagaataag agaaatggca gaaaaacaaa ccgcaggacg
      181 agatcaatta ggagattttg caccaaaatt tgcagaatta aatgatgatg ttttatttgg
      241 acaagtatgg tccagagaag aattatctgc tcatcagcgt agtatcatca ccatatctgc
      301 attaatttca ggaggaaatt ttgaacaatt gccagctcat ctgaagattg gcaagcaaaa
      361 tggcattact agtgaagaaa tagcagaaat cattacccat ttagcttttt atgtagaatg
      421 gccaaaagct tggtcagctt ttagtttagc aaaagaaatc tatttagaag aaaatagcga
      481 agataagaaa gaggcgtaaa tgatgacaaa acacgaagaa atacacaatg gcgtgatttt
      541 tccagcaggt gaaaaaaacg aagcttacgc tcaagtattt accgggcaaa gctatctgaa
      601 tatgttagtg aatgatcccg aagtttctgt tggtgtaggt aatgtaactt ttgaaccagg
      661 ttgtcgcaat cactggcatg tccatacaga cggctatcaa atcttacttg tgaccggagg
      721 agaaggctgg tatcaggaag aaggaaagcc agcaagacga ttaatggctg gagatgtcgt
      781 cgtaacgcac aaagggatta aacattggca tggtgcgaca aaagacagtt ggtttgaaca
      841 tttggcgatc actgctggta attcagagtt tttagaacct gtaacagatg aagtgtatga
      901 aggattggaa aatcaataat gtcaagcttg aacagctcct gaaagtagat tttaatcgaa
      961 ctttcaggag ctgctttttt gattcagtct tttataaata gaaaataggg ttaataatag
     1021 gaatgagaag cacgtcgaaa gatcttaata ttgataatcg gctgggcata catgatttcc
     1081 acgtcatcaa tcaaactacc taacagatga taatcttccc gatcatgtcg gggattacct
     1141 aatagttctt gatagtagat ttccatacta gggtcccgag gattgttcag caaatcagca
     1201 agtacatcta aatcttctgg ctgagtttct gccgtaccct gtatctctat gaaaagccct
     1261 tcctcagcaa attctactcc aatacgtatt tcagttattt catgggaata taaataagta
     1321 agaagctcat cgatgattct ttttgataat tctttttcca agtgtctttt tttcctttct
     1381 gacagcggta aagttatcta gtatcggtac aaggatacct gcaacgaaac cagttgaaaa
     1441 accactgtta tatagattta gtcctccgtg caaagtgctg acgttatgga caagagcaat
     1501 atgtagcaca ccagctatta caccgtaaaa actgccgtaa aaaccgctta tgggcgcaag
     1561 acctgtccca aacacagctg caacaagcat gccagtactg gtgatctcat gaaaagttcc
     1621 aaacaaagaa gcaataaata ttccgactag tatcggaaaa ctgtttttta aatgacaacc
     1681 aaaagcactg aaacccaccg cagataaaat agcaccgatg acaggtccat ttaactgacc
     1741 gccaaccagc aaaacataag aagacagcat cagaccaact aagcccatgt tgatcaaagt
     1801 agcaccaata ccagaattag caatgaaatc agttgtcaat tttccagatg aatcgaaaat
     1861 ttctcgaatc cccgaaagcc gaaaggaatt ataatagaaa ccaattccga ctgtcagcga
     1921 aaaaaagcca aaaataatcc aaatgagagg aaagtgatat tcattgaaaa ccaatgtatt
     1981 ttcgacgatt gaatagctga ataacctcaa aacagcagta aaagtcatcg caatcaaccc
     2041 agaggtgaat ccaatattat aaagtgtgaa tccacgatgg aaaactaaag tttgagcagc
     2101 taagggcgga agaagtatac caattgctat gccagctaaa ttccccaata aaatacctat
     2161 cagtaaagga aaacgcatac caaaagtgat ataactaacg accggtgata atacgctacc
     2221 aaacagtccg atcatgatgt actgagaaaa aggtttattg acaaatacag cgtatagata
     2281 cacacctaaa atgattgaga tagaattata aaggtttttg ccaaaaaaag agaaaccggt
     2341 aactgttaga atcgaagcaa ataagggacc gtttaaaatc accttgtgcc gataagctaa
     2401 aagcacagaa agtaaggtca aaatccctac atttatgaat gcgcttccga aaacacctaa
     2461 agcaaaataa tctgtcaaaa gattgcttgg cgaggtaaga atagttagca atccactcca
     2521 attatcagta ggagtattag aaagcaagcc aataaaaaga aaaaaaacac ctgtaaaacc
     2581 aagataaaaa tactttcttt tctgtgaaac gattcgtttt tgaggatgaa tataaataac
     2641 gtcagtttcg ttttccatta cacaccttct ttttcatttt ctttaactat aacttgaaat
     2701 gaaagatttg gacaactatc atttaggtca aaactaaaaa tgtaagggaa cccattgaat
     2761 ttaaccaaat ttttttaatt ttgcaaaaaa aagttaaaga agactgaaaa aaatttaaaa
     2821 tataacgttt tgagtaaaat gtttgcaaaa aatgctaaaa atagggtaca ataaaacaac
     2881 atatactgca gtaaagaagt gggggagttt taatgagaag aaaagtctat accaaggatc
     2941 aaattctaaa agctgcttat gatgttgttg caaaggaagg gtttaaagga tttactgcga
     3001 gaaatattgc taaaaagatg gggatttcga cacagccgat ttatctggaa ttcaaaaaca
     3061 tggaagattt aaaaaatacg ttgctggatt ctatctttga acaactaaaa gaagatgttt
     3121 tccctattga acgtactggt gataaaatca ttgatctcgg tttgaactat atctactttg
     3181 caaaaaagca tcatagtctt tacatggcat tgtacattga tgaacatggg gacggaaaga
     3241 aaatgcatga tttttcatac aattatttcc gtgaaatagt taaagaagat gaaagataca
     3301 atcaattatc ggatgaatat gtcgatgctc tgcatactgg tacatgggta gcagtgactg
     3361 gcttagcgtc attgatgtca tcaaacatca tgcatccaac agaagaacaa atcattgcac
     3421 tgatcaaacg gacaattgac tcgattttag cattagataa tcctgaaata atcagatagt
     3481 gttctatcag tatgaatttg atcggtaagt ccgttggctg caaactgtca atggactttt
     3541 ttgctaaaaa aagcgatttc tgttatttat ctattagaaa taggcttgac atttcgtgaa
     3601 aaaagtttaa aaatagtact tacggaaggt tggcagagtg gtaatgcacc ggactcgaaa
     3661 tccggcgaac cggctaatac cggcgcgcag gttcaaatcc tgtaccttcc ttatatcaac
     3721 gattagaggt tgtgaagaag ctgtcctgca tcaagtaata agaac
//
LOCUS       DACCXO010000163         3736 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.170, whole genome shotgun sequence.
ACCESSION   DACCXO010000163 DACCXO010000000
VERSION     DACCXO010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.170"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            85..687
                     /locus_tag="IVY70_002701"
     CDS             85..687
                     /locus_tag="IVY70_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959789.1"
                     /translation="MEDIILRDRLTDPLKNSLANVIYDILREADKVYSNYNGKVSWVI
                     YSFLIKEFFKEQINNSYELGYHFTVYYEDIIKRLEDLEWYAYYDLLEVFASAKILDKK
                     KREEVNKILESENSGYRLSESNEFIPITDDISIKSINAVSESPFNYAKNHIHKSLTYI
                     SEKENQDYNTVVREAISAVESCLIEFSGLPANKKILWELL"
     gene            672..905
                     /locus_tag="IVY70_002702"
     CDS             672..905
                     /locus_tag="IVY70_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959790.1"
                     /translation="MGTAIKAIKDKSNNNVDLTFLKPFETMYGLASNNGIRHAENEKT
                     IVSDLPDAVLVLTTCSAVINYLGVKIAYDSNKS"
     gene            1042..1278
                     /locus_tag="IVY70_002703"
     CDS             1042..1278
                     /locus_tag="IVY70_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP7959791.1"
                     /translation="MNIKKRKIREVGNSIVVTLSKESLLQKGLKPGDTIFIDEDKMME
                     AIVKEESKIDLEIDMYVNQALSEYDAAFKELVEK"
     gene            1275..1688
                     /locus_tag="IVY70_002704"
     CDS             1275..1688
                     /locus_tag="IVY70_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP7959792.1"
                     /translation="MIYLSEKDIVKLNVIQIKKYSPRETIGVIDKNALSMAVNQPKQH
                     IFGKDLYPDKFSKAAVLLINLIKKHPFHNANKRTAFLAAYVFLKLNGYSLKMKNQEVV
                     EFVVEIATYQGDFDDLKEATTDILKKNSTKTDSKK"
     gene            2207..3157
                     /locus_tag="IVY70_002705"
     CDS             2207..3157
                     /locus_tag="IVY70_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HAP7959793.1"
                     /translation="MERNSENKMTGRNPKVFISYSWENKENKEWARELADKLLENGID
                     AYIDQYDLSLGDRLPQFMEQQISDSDYVLIICTPEYKKKANNRTSGVGYEGHIISAEL
                     LSGKNERKFIPIIKEGRASEVLPTFLAGKLGIDLSDSQHYDENLDDLITTIFGVKRKP
                     ALGDIPRHIKGSPLLPIEHNENKDIEILGIITDKVTVPKMDGSRGSALYKIPFRLNRV
                     PSQLWKELFVRNWNSPPRYTTMHRPGIASVVDDEILLNGTTIEEVREYHRETLKLCIE
                     KSNTQEKKILLEEEQKKERENERVRSHKESVQKLADELEF"
     gene            complement(3154..>3736)
                     /locus_tag="IVY70_002706"
     CDS             complement(3154..>3736)
                     /locus_tag="IVY70_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7959794.1"
                     /translation="SIDQNEQRQIEEMRKFGAEKIFIEKQSGATITHRPIFQEVLDFV
                     RDQDIFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITNLPIMAEAIGNPLLDKFIKD
                     LIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANTKDPQRRLVYRSIV
                     QDQENGVAISKIATDYNVTRQTVYRIKKELTIN"
BASE COUNT         1362 a          498 c          672 g         1204 t
ORIGIN      
        1 tattgcttcg taaagttata tgagtataag ttaatttact ggaggtagtg caatgctttt
       61 ttctgaaaga tttggtttta aaaaatggag gatatcatac tgagagatag attgacagac
      121 cctcttaaaa atagtttagc aaatgtgatt tatgatatat tacgagaggc tgataaagtt
      181 tatagtaatt ataatggaaa agtctcatgg gtaatatatt cttttttaat taaagagttt
      241 tttaaagaac agataaataa tagctatgag ttgggttatc attttactgt ttattatgaa
      301 gatattatca agaggttaga agacttggag tggtatgctt attatgattt attagaggtt
      361 tttgcaagtg ctaaaatctt ggataaaaag aaaagagagg aagtaaataa aattctggag
      421 tcggaaaatt ctggttacag attaagcgaa agcaatgaat ttattccaat cacagatgat
      481 atttctatta agtctataaa tgctgtatct gaatcaccat ttaattacgc aaaaaatcat
      541 atccataaat ctcttacata tataagcgag aaggaaaatc aagattataa tactgtagtg
      601 agagaagcaa ttagtgctgt cgaaagttgt cttatcgaat tttcaggatt accagctaat
      661 aaaaaaatac tttgggaact gctataaaag caattaaaga caaatcgaat aacaatgtcg
      721 atcttacatt tttaaaacca tttgagacaa tgtatggttt ggcgagtaat aatggaatac
      781 gacatgcaga aaatgaaaaa acaattgtct cagatttacc tgatgcagtt ttggtgttaa
      841 caacatgttc agccgtaatt aattatttgg gagtaaaaat agcatatgac tccaataaat
      901 catgaaaagt taaaattcat ccaagagcta cattataact attaatttcc aactctttaa
      961 aagtttagta attgaaggaa gtaataaggg tgattgaaca atacggatat acggtgtata
     1021 cttatcgtag gagttgatga tatgaatatt aaaaaacgca aaatcagaga agtaggtaat
     1081 tcaatagttg taactttgtc aaaagagagt ttattgcaaa aaggtttgaa acctggagat
     1141 actattttta ttgatgaaga taaaatgatg gaagctattg taaaagagga aagcaagata
     1201 gatcttgaaa ttgatatgta tgttaaccaa gctttatctg agtatgatgc agctttcaag
     1261 gaattagttg aaaaatgatc tatctaagtg aaaaagatat tgtaaaattg aatgttattc
     1321 aaataaagaa atattcaccg cgagaaacaa tcggagtcat tgacaaaaat gcgctgagta
     1381 tggctgtaaa tcaacctaaa cagcatattt ttggaaaaga tctgtatcct gataaattta
     1441 gcaaagcagc tgttctgcta ataaatctga tcaaaaagca tccatttcat aatgctaaca
     1501 aacgtacagc atttttagcc gcgtatgttt ttctcaagct aaatggctat tcactaaaaa
     1561 tgaaaaatca ggaagtagta gaatttgtag ttgaaattgc tacgtatcag ggtgattttg
     1621 atgacttaaa agaggcaaca acagatatac taaaaaagaa ttcaacaaag accgattcaa
     1681 aaaagtagaa gcaaactagg gattcagtta aatctgaaga tttagtttgt tctttatttt
     1741 agtttacata agatacatta tgcgaagttc attattttaa aagcttaaat aataaacttt
     1801 tttataatac caatttattt atgtaaacca atgaccttaa tcgcttaagc aagcatgtta
     1861 tgtaatactc tactctctcc aaaactaatg actatctatt ttcaaagaag ctagaatgat
     1921 tatctgatca aatgataatt tatatttaat tcctggtcta ctgctcttct tttaatcaat
     1981 taatgtctaa cgtactgtac acgataaagt aggaagactt attttcaact tcggattcat
     2041 ttttttaatt cggtgaaaat cactaaatta agagtttatc caagttcatt ttagctgaga
     2101 atacgcttga aaataatttt gatgaatttt cctgaattaa aatttattaa cttgttgagt
     2161 gatataattg taatgtatta ccaaatagtt agcacttaga ttagtgatgg aaaggaatag
     2221 tgagaacaag atgactggta gaaatcccaa agtatttata tcatattcat gggagaataa
     2281 ggaaaataaa gaatgggcta gagaactagc agacaaatta cttgaaaacg gtattgatgc
     2341 atatatagat cagtatgatt taagtttagg tgatagatta cctcagttta tggagcagca
     2401 aatatccgat tcagattatg ttctaattat atgtacacca gagtataaaa aaaaggcaaa
     2461 taatcgtaca agtggtgttg ggtatgaagg tcatattatc tcagcagaat tgctttctgg
     2521 aaaaaatgaa cgtaaattta tcccaataat taaagaagga cgtgcaagcg aagtacttcc
     2581 tacttttctt gctggaaagc ttggaattga tttatcagat agccagcatt atgatgaaaa
     2641 cttagatgat ttaattacaa ctatatttgg tgttaagaga aaaccagcat taggtgatat
     2701 acctaggcat attaaaggca gtcctttact acctatagaa cataatgaaa ataaagatat
     2761 tgaaatctta ggaattatca ctgataaagt aactgtacct aaaatggatg gctcaagggg
     2821 ttctgcatta tataaaattc catttagatt aaatagagta ccaagtcagc tttggaaaga
     2881 gttgtttgtt agaaattgga attcaccacc acgatataca acaatgcata gaccaggaat
     2941 tgcttctgtt gtagacgatg aaattttact taatggaaca actattgagg aagtccggga
     3001 atatcatcgg gaaactttaa aactttgcat tgaaaaaagt aatactcaag aaaaaaaaat
     3061 attgctagag gaggaacaaa aaaaagagag agagaatgag cgagtcagat cacataaaga
     3121 aagtgttcag aaattagcag atgaattaga gttctagttg atagtcagtt ctttcttaat
     3181 tcgatacaca gtttgtctag ttacattata gtctgttgct atttttgaaa tagcaacacc
     3241 attctcctgg tcctgaacga tgcttctata aacaaggcga cgttgaggat cttttgtgtt
     3301 cgcactgtat aacttaggac gtcctttata gacgccgttt gctttagcaa tctttatccc
     3361 ttgtgcctgg cgtcgttttg attcagtacg ctcttgttca gcaatcatgg ccaagatttg
     3421 aatgattagg tctttgataa atttatccag gagaggattt ccgattgctt cagccatgat
     3481 cggtaaatta gtgataataa gtcgcacatt tttcttcttt agataattta ctgaatcgat
     3541 aatctcgtca taattacgtc caagtcgatc gatagcctct acaataaaaa tatcttggtc
     3601 tctaacaaaa tctagaactt cctgaaaaat cggtcgatga gtaatagtcg ctccagattg
     3661 tttctcgatg aaaatttttt cagcaccaaa ttttctcatt tcctcgattt gacgttgttc
     3721 attttgatcg atcgat
//
LOCUS       DACCXO010000164         3711 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.171, whole genome shotgun sequence.
ACCESSION   DACCXO010000164 DACCXO010000000
VERSION     DACCXO010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.171"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            190..1905
                     /locus_tag="IVY70_002707"
     CDS             190..1905
                     /locus_tag="IVY70_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7959795.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            1908..3671
                     /locus_tag="IVY70_002708"
     CDS             1908..3671
                     /locus_tag="IVY70_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7959796.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYAFEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
BASE COUNT         1105 a          728 c          737 g         1141 t
ORIGIN      
        1 aagagactgg catgttttct cattttcttt tttttaaaaa aaatagtcta ttcaatttct
       61 aaagcttctt cataataaaa ctttcattta tgaaaaggct tgttctcttt tcattctaaa
      121 tttcctaagc tacaatgtgg tagtacttgt tttttcaatt gtggacaaat aaataatagg
      181 aggaatcata tgattcagtt attacgttat gcaaaagact atcgtaagca aatcattttg
      241 ggcccgttct tcaaatttct tgaagctgtc ttcgaacttg tcctgccttt aatgatggct
      301 tccttgatcg ataatggact aaaaatgaat gaccgaggaa aaatcattga aatgggctta
      361 tggatggtcg ctatgtctgt gattggtctg atttgtgcta tcatttgcca atactatgct
      421 tcagtagctt cacaaggatt tggtacagag ttacgtaatc agctaatcaa aaaaatcaat
      481 actttctctc acaacgagtt gaatcatttt ggaacagata ccttgatcac ccgaatgaca
      541 aacgacatca atcagcttca gctagcttta gcaatggtca ttcgtttatt gatcagagca
      601 ccgtttttaa gtatcgggtc agtggtcatg gcttttgtga tcgactggga agtaggtctg
      661 tttttcttag cattattgcc tattttctca atcattttat tttttatcat caaaaaaacc
      721 gttcctctct accaaaaagt gcaagaaaaa ctagatcagc tcaatgaaca cgttagtcag
      781 aatcttagcg gtgttcgtgt catccgcgca tttgcgcaga caaaaaaaga aacagagaag
      841 ttcgataaaa gcacaaatga tctagcgaac aattatttac gtgtttccaa tatctctgcc
      901 ctattatcac ctgctacaac cttgatcatg aatgtcggga tcatctgttt gctgacagtt
      961 ggcgggatca aagtaaatat tggtagcttg caacaaggac aagttcttgc tttgatcaat
     1021 tatatgaatc aaatgctttt ggcactgatc gttgtttcaa acctcgtcgt gatcttcaca
     1081 cgtgccgaag cttcaggaaa tcgggtgaaa gaggtgctag acacagaaaa ttcgattctt
     1141 gaagctgagg tcagtactac tcccgatttt tcttctgaag caattatcgc ttttgaccat
     1201 gttgacttcc gttatactcc tgattctggt ctttctctcc aaaatattac atttcagctg
     1261 aagcgaaata cagtccttgg aatcattggt ccaaccggaa gcgggaaaac gactttgaca
     1321 caattgattc cgcgtttcta tgatgtcagt tccggtacag ttataatgga cggaatggat
     1381 gttcgttctt ggcagctgga tcatctccgt cagcaaattg ctcttgttcc ccaaacttct
     1441 gtactattta ccgggactat tcgtgaaaac cttcaatggg gtaagaaaga cgccacagaa
     1501 aaagaatgtt gggaagctct gaaaatcgct caggcagaag aatttgtccg tcagcttcca
     1561 aatggtttag atacaaaagt catggaaaat gggaaaaatt tctctggcgg acaaaaacag
     1621 cgactgacga ttgcaagagc tttgattgcc aagccagcat tactgatttt agatgactct
     1681 ttaagtgcgt tggattatca aacagacttg gatttgcgta aggcgctaca gacttatctt
     1741 ggcagtacag tgattattat ctcccaacgt gtccgctcta ttcaagaagc acatcatatc
     1801 ttagtgatgg atcaaggtaa gattgctgct cagggtaccc atgaagaatt acttgctcaa
     1861 tcagcagaat atcaggaaat cgtcgcatca caggaggaag aatagttatg aaagtaaaaa
     1921 aattatcctt gcagacgctc cgccgattca ttccttacct tactgcctat gcaaaagaca
     1981 tttggctagc tgtgatacta ggaatcatca atgggattgc aacagttgtg atgacgttac
     2041 aaattggtca aagtatcgat caaatgattg gagaaggaca ggtccgcttc aatcgactgt
     2101 tccatttact tattctattc ggtggtatcg ttttaatcaa cgtagtcagt caatggctca
     2161 tccaagtgtt aagcaatcgg ttagcttact tatccgttgc aaaattaaga aaagatgcat
     2221 ttgctcatct gaatcgattg ccacttcgtt attatgatca aaactcccac ggaaatatcg
     2281 tcagtcgatt tacaaatgat atggacaata tttccattgc aatttctgcg gccttcaatc
     2341 aattattttc tggagtggca atcatcattc tttcgtttat ttttatgatg cgtctaagtc
     2401 cacttctgac attggtcgtt ctttgttcta caccaattat tttccttgtg agttggatcg
     2461 ttgctcgtgc ttcacaaaca aatttcgata gacagcaaag gattattggg gatatctcgg
     2521 gctttgtgac cgaacgaatc ggtaaccaga aaatcgtgaa agcttttcag caagaagaag
     2581 ctagtcaaca gcaatttgaa ctgctaaatg ctgatctata tgaaaaagga caacgggcac
     2641 aattttcttc ttccttgaca aacccgtctt ctcgttttgt agatcacctt gcttatttat
     2701 cgattggttt agttggcggc ctattggcat tgaagagtgg ttcgtcaatc actgttggtg
     2761 tgatttccag ctttacgatc tactcaagtc agttttctaa accatttatc gaactttctg
     2821 gaatcacgac acaactgcaa accgcttttt ctggtctaga acgagcattt gatttgatcg
     2881 atcagccaga agaaaagcct gatttgccag atgcctatgc ctttgaaccc aaaaatatca
     2941 aaggaaaagt ctcttttgat catgtttctt tttcttatga ccccaagcag cgtttgatcg
     3001 aagatttcaa ttttactgct tatccaggtc aaaccattgc tatagtagga aaaacagggg
     3061 ccggaaaatc cacacttgtt aatttgctga tgcgttttta tgacacaaca aaaggaacaa
     3121 ttacagtcga tgaccatgac atccgctcta tccaacgtga tacattacgt aaaagttttg
     3181 gaatggtgtt acaagatact tggctatttg acagtacatt gcgagaaaat cttcgttttg
     3241 ggaatccgga agcaagtgac gcagaaatca atgatgccct aaaagcctct tatatgtatg
     3301 aatttgtaga acgtttgccg gaaaaattag atactaagat tggcggtcaa gggttaaaga
     3361 tatcagatgg acaacgccaa ctgttgacga ttgctcgaac gatgatcagt aatcccccta
     3421 tgttgatctt agatgaagct accagctcag tcgatacatt gacagagaaa aaaatccaac
     3481 aagccttctt agcaatgatg gaaggaaaaa ccagttttgt gattgcccat cgcctgtcca
     3541 caatcaaaaa tgcggaccag atattagtga tggatcaggg agctattgtc gaaatgggca
     3601 cccatgatga gctgcttcaa aaagatggtt attaccgacg gctctatcaa gcacaattca
     3661 cagcacaata aaaacaagca gtcttctggg atgggaagac tgcttgtttt t
//
LOCUS       DACCXO010000165         3657 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.172, whole genome shotgun sequence.
ACCESSION   DACCXO010000165 DACCXO010000000
VERSION     DACCXO010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.172"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            27..479
                     /locus_tag="IVY70_002709"
     CDS             27..479
                     /locus_tag="IVY70_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959797.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            493..1182
                     /locus_tag="IVY70_002710"
     CDS             493..1182
                     /locus_tag="IVY70_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959798.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1307..>3657
                     /locus_tag="IVY70_002711"
     CDS             1307..>3657
                     /locus_tag="IVY70_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP7959799.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
BASE COUNT         1243 a          476 c          767 g         1171 t
ORIGIN      
        1 tttaataaga aagcgaggtg agaatcgtga aaaaaacgat actaagtatt ttttcagtat
       61 ttacaatatt aattgggggc tattttcttt tgaatttaga ttctgttaat tcaaacgctc
      121 aaataaaaga tcaaagagta gctgaacata ataataaaaa aatgagtttg accaaaaata
      181 tctattctat gtcagatgaa gaattgtata aagaatttgg tgatttatcc ggggaacagt
      241 tagaagaaag tattccagga attagtgact cagataaaat tgttgtcttg tctgctggtg
      301 ggcaattaat gggagatggc aaatccgaat tagttagtta taaacaaaat cacagacagt
      361 ttattgatgt taatactgat tccaacgcta tttcgattaa ggatctaaaa gagcttttaa
      421 atagtaaaag aaatgaatta aagcagataa aagaaactaa ttcagtggaa ttagagtaaa
      481 ggaagattca ttatgaaaaa gatttttggt gtttctgttg ggctacttat gatgttaaca
      541 tttggaaatg cagtatatgc tgctgagaaa gaagttattt cagatccaga gcagtacgcc
      601 tcacgatact ttgctgattt tgaagaagaa gataaagtac ttgcattgcc agaaggtggt
      661 tttttaaaag gggaagctaa aattgtatct atggataatc cagatataat tcaaggttca
      721 tataattcag aaactgatcc tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct
      781 gaaaaattca aaaatgatat tgaaactact ccaattatgc ctcgaggagc atttccaccc
      841 gatgttggta ctagcggaca ccttcttgta ctagcaggag gaggaagcta taaatcacag
      901 ccatttagtg gtgcgggatg gcgttttgga ggtatgggtg taaaaccagc tgatggtaca
      961 ggcaactttt tacgttggaa atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa
     1021 gccaacgcaa ctaaatctgg ttctattcaa ggaatggcat tgtatgctcg tgaaggattc
     1081 aaatactttg caccaaacgg gcctggttgg acaatgaata taggtattta ttacacttat
     1141 aatcctgttg caggtacgta ttatgttgtt caaaatattt aataaaacag cacaaagaga
     1201 tttctttgtg ctgttttatt atttttagtt gttgacaaac actaaaatat aaaatataat
     1261 caacacataa acgaacgtat gttcggcaag aaagcagggg atttttatgg atgagatggt
     1321 tttaagtact cagaaatggc taaacaagaa atacagtaat gttacagggt ttgataaggt
     1381 tccagaaaat gggcgaacag ggtggcctac aatctatgga cttattgaag gactacaagt
     1441 agaacttggt attactaatc tagtagctaa ttttggacct accactgaaa aaatgtacga
     1501 taatcaggtt actcctaaat ggggcaaaaa tctacctaaa aatattgtat ttcttattca
     1561 aggtgccttt tggtgtaagg ggattaatcc aggtggcttc gatggggtat atactccata
     1621 tttagatact gctgtaaaag agttgcagac tgatgcaggg tttaatggaa gcgctgtaac
     1681 tggagttttg gatgcgaaat gggccaaagc gttatttgat atgagtgctt ttgttcttgt
     1741 tcccggcgga gatagtaaaa ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga
     1801 atacacggga ataatgcctt gtgatggtat ttatcaaaga aattcaaatc aagcattaat
     1861 ttttgcatta caagctgaac taggatattc tcccagtgaa gcaacagggt cttatggtaa
     1921 tgggacaact agtaaaactt atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat
     1981 aattcaatat ggattatatg ttaatggtta ttttgaagac ggtacattcg atggtatttt
     2041 cactaaatat atgggtttag aagtactggc attccgaaaa tttatggtat tgcctgaata
     2101 tactgaaata gcagatgttg ttgtaataaa aggactattg tctagcgctg gagatattag
     2161 aagatctgca gatggcgcag atacttctac gcaattaact agatctcaaa tccaaacatt
     2221 ggttgataat gatataaaaa tagttggtag atatcttact ggtactgttg gaattggaaa
     2281 agatgaacga aataagtatc taactacaga ggaattgaat aatatttttt ctaaaaattt
     2341 atctgtgttt cctatctatc aagatggagg tgcagcatta gcttatttta cttatgatcg
     2401 tggcttatca gatggtaaga aggctataga tgcagcaaaa aatctaaata taccattaac
     2461 aacagttatt tattttgctg ttgatctaga catgttaggg gaagaaattc ttgcatatgc
     2521 tggagaatat tttaaggggg tatctgctgt aatgtcatat agcgggtatc aaacgggtgt
     2581 ttacggaaca agaaatgttt gttctcaaat aatcaataac gggtatgctt cgtttgcatt
     2641 tgtttcagat atgtcgactg gttatagtgg gaatttaggt tttcctatgc ctagacaatg
     2701 gtcttttgat caatttgttg aatttaccat aggaagtggc aacggagctg ttggtatcga
     2761 tttaatcgct acatcgggta gagatagcgg atttaatgag ttatcaaatg atactaataa
     2821 tgataattac attgccaaat acaatcagaa ggttataaat cagatggtac gtgcgtatca
     2881 atatctatca caagctggtc ttgataatcc ttggaaccct catttgacat tttatagata
     2941 tgtaaattat tctggtttgt catgggacat catttcttct cctgtaacag agcatgatag
     3001 aaaaatttat gatgaatata ggaataaatt aactaatagc gagggcctgt ataattattt
     3061 tatagatcct aatactggtt caataatagg tttgcctcac ttaattgtga cgttacaatc
     3121 acagatgttt ctagccgaca caatcaatga tagtgtttct gactttgcag gttggttagg
     3181 agatttaatg acttgctggg gtgaagttaa gaaattagga atacaaatgg agcaaggtgt
     3241 atttgcatta gtaggtacgg ctgcaggtgg aacatttagt ctagaagatt tataccaaga
     3301 tgttgacgcg gtaaatgttt ataccttagc aaaaagaaat tctgattcaa cagctttatc
     3361 ggctttaaaa acgtactatt gtgataaggg tgctattgat agatttaatc aatttgttaa
     3421 gaacagattt ggttcttcag ataaaatcgc ttcagtaaca aatagtttat tagaaaatga
     3481 cgaaacagtt gatatcttag gtgtagggat aatttcattt ttcattgata aaatagttaa
     3541 taattcactt gggacagatt ttacttatct tgattttatt ggagatgagg ggaaagttgc
     3601 aaatggattt tctgaaaaaa ttattcaatt tcaagaaaat agaagtaaac ttaatta
//
LOCUS       DACCXO010000166         3584 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.173, whole genome shotgun sequence.
ACCESSION   DACCXO010000166 DACCXO010000000
VERSION     DACCXO010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.173"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            958..1926
                     /locus_tag="IVY70_002712"
     CDS             958..1926
                     /locus_tag="IVY70_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP7959800.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            1936..2424
                     /locus_tag="IVY70_002713"
     CDS             1936..2424
                     /locus_tag="IVY70_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP7959801.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            2720..3001
                     /locus_tag="IVY70_002714"
     CDS             2720..3001
                     /locus_tag="IVY70_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959802.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            3003..3419
                     /locus_tag="IVY70_002715"
     CDS             3003..3419
                     /locus_tag="IVY70_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959803.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
BASE COUNT         1321 a          629 c          601 g         1033 t
ORIGIN      
        1 aggtatttgg ctagcaaata tagagcttgc caagcccttt agtgtgtttt gtagaaaggg
       61 accttatagt cactttcaca ttacaaacga tacgtcctat ggacgtatca aaagggtggg
      121 acttatgagc aaattaaagc aaaatttggc tagcaatcct tctaaaaaat acacctattg
      181 atcagagtca aaataaatca gttttcgagt gagcgaatcc aaatttgcaa ctctgaagct
      241 ttgcaaatga gtgtgccggc ttccgtaaaa gtctaggtaa cattagtcga tatatacata
      301 gggctctaat ttgcgtttta aggcgtttta aacgttctgg tatgtaatta taccaaagcg
      361 actttaaaac gccttaaaac gaattttaga ggttctgtag aacgttttag ggttgttagg
      421 gcttgccctg accgcctgta aggcgcttta aaaacaagat aagggatttt atctagactt
      481 gtcttaaaac gccgtagatg agcgctcaga gcttctaaaa ggactcctta cgaactgttt
      541 ttaagcaaag gagcctttag gcgactgcgc agtttaatgt gagagcgtag cgatcacacc
      601 tttaaagcga ccgtagggag cagattccca gtttaggaaa tttcgcgaac ctgtttggag
      661 aaaaaaagta ggaaaatttt aggcgggaaa ttttgccccc ctcttttata atctctttta
      721 taacctcttt ttaaacctct ttaccgtcgg ctgtatcctt actctcccaa ggtgtgcaga
      781 atcttttagg ggtctttttg tctgttttta ggggtctttt tgtctgtttt taggggtctt
      841 tttgtctgtt tttaggggtc tttttgtctg tttttagggg tctttaatta gggggttgac
      901 ccccattaga aagctcttta taattttgtt aagtagttaa tcttgaagga ggattttatg
      961 gcaaatgaaa ttgtgaaata ccaccatgaa ttaaatacga ttcctttacg caaatttacg
     1021 tcagtagaaa tgaatttatt cttctctata atatctagaa tgcgtgatgt aggcgataaa
     1081 aaagtacaat ttacattcga acaattaaaa gatttaagca attataaagc aactgcaaat
     1141 gttcgattca tagatgattt agaaacaacg tatgacaaat taatggattt acgttttggt
     1201 agaagaagtg ctgacggttt gcaacgtgaa cgatttgttt tatttaatca atttaaaatc
     1261 gatggaaaag ctgatattcc ttttgctgaa atacaagtcc atgagaaagc cttaccctta
     1321 cttaataatc ttgaagaatg ggttcgatat tctctccaac aattcaatga attagaaagc
     1381 agttattcca aaaccatgtt tagattatta aaacaataca gaaccaaagg aattgccaat
     1441 ttttcaaaag aagactttca tgaattactt gatatcccta aaagttatag agaaagtgat
     1501 ataaataaaa aaattataaa gcctattcgc caagaactaa ccgcaatctt caaaggtttg
     1561 acaatccaaa agagatacgg aaaaggacgg ggaaagcctg tcattggcta tcaatttaca
     1621 tttaaacctg agataaaaaa atcagacgac tttaacaaac atgctccaac caaagaagaa
     1681 caaaataaag ctttggaact agctaatcaa caggttcaat ctgaaaaaga acctacttca
     1741 ttacaagaaa aaatcaaaga acaaaaacga aaattagaac ttttacaaca gttagcagaa
     1801 ttagaaagaa aacaaaatac cccaagtaag aaaattaaca aacaaatagc agataacaat
     1861 tccgatatta ccccagaatt taaagaaaac ttactagata atctccaaaa cttatttaaa
     1921 gaataagggg tttttatgac agataaaact atcaaagaat tagcagatat tctaggtgtt
     1981 tcaaaagata aagtgaagta tcaactgagg aaattaccta gtaattttac ctacaaaaaa
     2041 ggaaatatta cctacctgaa aaaagaagca gtaacaacaa ttacaaaggt tttatcaggt
     2101 aatgaatcgg gtaacttacc tagtgaatta cccgattcat tacctaccga ttacctactc
     2161 aaggagattg aaactaagaa taatcatatt gaagaaaaag ataggcaaat tgaaaaactt
     2221 caaatagctt tagaaacgca gcaaaaatta ctcgaccagc aacaacaact caccctacaa
     2281 gccaatcaac aaatcaaaaa acttcacgaa caactccagc taacctatgc agaagaatct
     2341 tcagaaaaag aacccctttc tgttactgaa gaaactcaaa aaggacaaaa caaggagaaa
     2401 aaatggtggc aattctggca ataacccaac aatttttatt cataaaaatt gttgggttac
     2461 tttctttttt tgcttttaca agcatagcga catgatctca cgtcgctatt ttttaaagag
     2521 cataaggaaa cgcggtatca ctttcttacg aaaggtgcta ggtaggctgt gcctaccacg
     2581 tttctaagaa aaaaatttgc tatcactttt tcgtcattgt ggatagctta tgtatagtgt
     2641 ttcggcaaaa aacagctata caaatactat atttgtatga tatacataaa ttatacacaa
     2701 atgcgaaagg aattcttcta tggacatttt agttcggaat attgatccaa aagttatttc
     2761 agaaatagat agacgttgcc aagaactcac aacaaaaact ggcaaaaaat ggagccgtaa
     2821 tgcgtaccta aaagttctaa tcgaaaacga ttttgaccag gcacttaggc attacaaaaa
     2881 agatcaattc gatttattac tcgaaaaatt tatcgatata caagcaaaaa atactgaaac
     2941 attgaacgac tatgttcata caaacaacca actcattgaa acacttatcg gaggaatata
     3001 acatgagaaa aacttctgtt caaaaacacg ttacccttta tcccagtcac attgacgttt
     3061 tagattccat catcgaaaaa aatccagata tcaataacta ctcggaagcc attcgttttc
     3121 tatgtatgag ttatgaacct aatactaata cgacaactac cagcaaccaa gcaaaactaa
     3181 atgcgatggg aaaagaaatt tctattatgt ccgaaatcat tgcagaattt gctgatttat
     3241 atctaaaaga tactggtgtt cttatcggta caaattctaa agtatatcaa gaagtgaaag
     3301 aaaaaattga aaatcaactc aatccctccc gacgaaatca acccaaaaat cgtcaaaaag
     3361 aaaccaatca aacattcact tctagaaatt ttgacccaac aaactttcta aaaagatgaa
     3421 aaagaaaccg aaaatttttt ctatttctca gtttcttttt tagggggcag tcttccagca
     3481 agatttgcaa gttaccggta acttgcacaa aaaaagaaaa atccgcttgt gcgaatcctt
     3541 ctttttgcat tttggggcgc tgccccaatc tccaaatatc ggtt
//
LOCUS       DACCXO010000167         3565 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.174, whole genome shotgun sequence.
ACCESSION   DACCXO010000167 DACCXO010000000
VERSION     DACCXO010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.174"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(1820..2338)
                     /locus_tag="IVY70_002716"
     CDS             complement(1820..2338)
                     /locus_tag="IVY70_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP7959804.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            3456..>3565
                     /locus_tag="IVY70_002717"
     CDS             3456..>3565
                     /locus_tag="IVY70_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7959805.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT         1225 a          612 c          530 g         1198 t
ORIGIN      
        1 gagagactac ggttgatttg atattaacaa gcgtatatga atgtatatac aaataaaaaa
       61 ggggagggag aagttaaaga aaattcagag aagtggggca ctcagtagta ttaacaattt
      121 aaaaggaatt tcttaaagaa aatgggttgc atcctggaga tacaattttt ttagataaag
      181 aaaaaattca agcaacaatt caaaaaaatc ttaactgctg agattgatgt agcaatatct
      241 caatcatcaa caaagtacga tcctgccttt caaaagctta ttgataaatg tttattcatt
      301 ataaaaataa aagaaaaatt tatgtcagct tttttcatca aagtggtaat gataattcgt
      361 ctattaattg tattttttag gaaatatggt ataattgttt taacgaaaag gtaattcatg
      421 atacaccaaa aaccactagc tggcgggcta gtggttttct tttacgttct attcagatta
      481 acgggtaggc ttactgtctt ttttggcaat aattttgcaa tcaagccatt tacagatgta
      541 atgcgcggat acttctgcta agattgcaaa aaacatatcg aaaaggtaat tcataataca
      601 acctcctccc cttgccataa taggccgggg tagcagacag cataaatatt ataccataac
      661 tgaaaatttc attttagtac atttcaatat attgatgaac cagcacaata aaacatgcca
      721 tgactaacag aaaaaaactt attaaaccag atataacttg taaacatcca actatataga
      781 aaacgtaaat tttctatata gttggattaa gataacgact atttatttta tcatattttt
      841 actataccct atacttttac ttggtatatt tatgaataat cgcaaattta cccctatact
      901 actagtttgt aagttagatt aataaaaata ctaaagttat ttgttctttt attaaataca
      961 cctacaactt ataaattgat ggtatttaga taacacgaaa gaataaaaat aacagctgat
     1021 agtaaagttt gttgacgatt gctactcaat cgaaagtgag ttagtgcgta tgaacttgct
     1081 tctgaaaact tatgaaatga aagacctttt acatcttgca gaaatcgtta ctgggattaa
     1141 actaagcaat aaaaactaat gttcccctca ttctttgaac ttctactatt tgatttattt
     1201 attctgataa atgacgattt caccttcagg atcttcaaat cccgtataga atttatttaa
     1261 gatctcttca gtatcagtaa taggataggc gataggccac ataccctttg atggttagaa
     1321 taaactgctc aaaatctgta tcgtatgttg gctttagcat attaacaaag gctatctcca
     1381 aattataaac cataagcgtt gaatcgttct taacaatttc aattcttcct aacccaaagt
     1441 cagaagatat aattaccgga cttgccttac ccttcatgtg ggttgttgag attccatatt
     1501 taaagggaat tgtcggtact atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt
     1561 tcccctgcta gtctcctaag tccctttgta gcggatatgg caccataatc tcattttatc
     1621 gaagtgagtc atggtttatc tgttggccaa ccaaaattga tacaatctaa cgatgcaccc
     1681 tctacggcat tctaggggag tgcacttgca ttcacccaat aaaaaccggc agttagcaaa
     1741 gaacttatgt agtcgtatgt tcttactgtt aacatacaag aggtctcctt tccacaattg
     1801 cttagcagta aacgggaatt tacaaagcta atctcttcca ttttcttatc tttgttctaa
     1861 aagtcccaaa tggagcaact gtatttacat gaataaattt atatacttcc catacagccg
     1921 ttttggttgc ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct tcatcactca
     1981 ttgaatcaat catcatgtta atatcatcaa tattacgatt gagaatttcc tctaattctt
     2041 tcaatggtag atgtgcatag gtatttgtaa accacttata taactctcct aactgattcc
     2101 atttaaattg atcagaaggc gttttaacag ataatccatt tctttcatca ttttcccatt
     2161 ttagaattaa attcgtccaa ccaacttgat atgctaaatt ttcagcaggt gttctatcca
     2221 cttcctcaac tcttttatcc ttcaattctt caggaatagt ttcaaattct aaaatatatt
     2281 tagtataagt cctatttatt tcttttttta attcttcttt atttttatat gttctcatta
     2341 tattacctcc ataaattcct aattgtctcc tttaattcat ctataatttc atccacgata
     2401 ttttaatccg ttctacaatg catatctgat atgcattgac tgtctaactc atatgtgaat
     2461 gatttataaa atatcccaac gtctcatcta aagaaatgtt gatcgtcagc aaggagctaa
     2521 aaaatacgaa tatatttctt ttgtagtaaa atcggactgg tttgcatatc attttcttcg
     2581 taaatatcaa aatcatgtta actactatca attactaata aaaactacca ctttaagcgt
     2641 taagaactgt taagtttagt taagtgagga tagttcacaa aatccattgt taaacttcac
     2701 gaaagtggta aaactcaaaa tgctttatat aggattacag catcgctctt tcttcgattg
     2761 cgcgttgggt aaaaaatatt atgaagtaaa aattgacgat gacacgttcc ttacggctca
     2821 tcagatgaaa cagctacaaa aacgaaatga tcaactcgaa gaaaatttaa ttctaaaaaa
     2881 gcgattgcca tattcacgcc tcgctcgacg aactattaaa ggtcaagatt tacctgaaga
     2941 tcatcctttt taggaaattg aagcttttta tgttgcacaa tgcatttata atactacaat
     3001 gatgttttca ccagatattc ttctagggtg cggtgtaatg aaacagatac acttgcaaaa
     3061 gaaaattcac cgcgaattta ctaaaattat gaataattat gttaatcttc cgaaattttt
     3121 ttccaaaatt aagggatcaa gctggtgtta tagattgttt aaggttggca aaaaaataac
     3181 ttctttttaa acaattatag aaaaccgata ctctgttaag ttggttgaca cggtgtcgat
     3241 ttttatgact ttcttattct gaaacttcac taacggacag tacctaaaaa gtctatagta
     3301 tggacgttta cgagtactat tttatcaaaa atgctgctca tttcaactta tatgttttag
     3361 tgaactaccc ccacttagct tggataggct acaattaact agacagaaaa attaaggtgt
     3421 gtagactaga agaaaacata ccaggaggaa tttttatgtc taagagaaca cgaagaactt
     3481 tttcacaaga attcaagcaa caaatcgtca atctttactt agctggaaag ccacgtgtag
     3541 aaatcattcg agaatatgaa ctaac
//
LOCUS       DACCXO010000168         3559 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.175, whole genome shotgun sequence.
ACCESSION   DACCXO010000168 DACCXO010000000
VERSION     DACCXO010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.175"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..280)
                     /locus_tag="IVY70_002718"
     CDS             complement(<1..280)
                     /locus_tag="IVY70_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAP7959806.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAI"
     gene            386..1063
                     /locus_tag="IVY70_002719"
     CDS             386..1063
                     /locus_tag="IVY70_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP7959807.1"
                     /translation="MNLVPLKNFNAKQMDLFFALCARMKDRGLKKVRFSFEELKELSD
                     YKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTKKFVEVSVNPD
                     LEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIEEFRSLMDIP
                     EYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKIS
                     LRNWLEE"
     gene            complement(1200..1616)
                     /locus_tag="IVY70_002720"
     CDS             complement(1200..1616)
                     /locus_tag="IVY70_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959808.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1619..1903)
                     /locus_tag="IVY70_002721"
     CDS             complement(1619..1903)
                     /locus_tag="IVY70_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959809.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            2247..2492
                     /locus_tag="IVY70_002722"
     CDS             2247..2492
                     /locus_tag="IVY70_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959810.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            complement(2794..2889)
                     /locus_tag="IVY70_002723"
     CDS             complement(2794..2889)
                     /locus_tag="IVY70_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP7959811.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
BASE COUNT         1147 a          570 c          647 g         1195 t
ORIGIN      
        1 cgatggctcg atacaaataa tgccattttc ctttaatttt gatgtacgtt tcatccattt
       61 tccatgaata aaaggatttt ttatttttct ttttccaaat ttgatagagt agtttgccat
      121 attcttgcac ccaacgataa atcgtcgtat gagaaacgtt aatgccacga tcatataaga
      181 tttcttgaac ttcacgatag ctaaggttat aacgaagata gtagcccacg gctacaataa
      241 tcacatcctg ctgaaattgc tttcctttaa aatgattcat cgtcattcct cctgctatct
      301 ttttctatta ttctacctta tttgatagta gatttaaaac tttgcaacag aaccaacgga
      361 attaacagtc aaatatcaaa atgaattgaa tttagtaccg ctgaaaaatt tcaatgctaa
      421 gcagatggat ttattttttg cattatgcgc tcgtatgaaa gatcgtggtt tgaaaaaagt
      481 tagattttct ttcgaggaat taaaagagtt aagtgattat aagatgactg ctacaaaagc
      541 ctttgtcggt gatttagaaa agctctataa agatatgctt aatcttactt accgtactga
      601 agatgaggat tctattcgct actttgtttt gtttactggt tttgaaatca ataaaacaaa
      661 aaagtttgtt gaagtcagtg ttaatccgga cctagaatat attattaatg gtttaacagc
      721 tgaatttagt agatttgaat tatctgcttt cacgtcgttg cgttctacgt atgcaaaaac
      781 tctattccga ctgcttatgc agtaccgttc tactggttat tatgtagttc gtattgaaga
      841 gtttaggagc ttaatggata tcccagaata ctatcaaatg ggtaacattg atcaaaaagt
      901 attaaatcct gcattacgag aactaaaaaa atacttcaat gatcttaaag tcacaaaaat
      961 aaaagcaaaa aagggcaata aaatcgccaa attagaattt actttttcaa gcctaacgtc
     1021 agatttgcca aagatttctc tgcgtaactg gttagaagaa taaataaaaa agaactcgcc
     1081 ccccttacat taagcaagta gacaagtccc ttacaaccga ctaagattgc tagtacattc
     1141 tagcatattg tatggggctt ctaaataaaa aacagatagg aataactcct atctgttttt
     1201 tatcgtttta gaaaatttgt tgggtcgaaa tttctagaag tgactgtttg actggtttct
     1261 ttttgatgat tctttggtcg atttcgattt gatggattta gtttagcttc gattttttct
     1321 ttcacttctt gatatacttt agaatttgtg ccaataagaa cgcctgtatc ttttagatat
     1381 aaatcagcaa attctgcgat gatttcagtc atgatggaaa tttcttttcc catagcattt
     1441 agttttgcct gattgctggt agtggtcgtg ttggtatcgg gttcataact catacataaa
     1501 aagcgaatgg cttccgagta gttattgata tctggatttt tttcgatgat ggaatctaaa
     1561 acgtcaacat gactagggta caaagtaaca tgtttttgga cagaagtttt tctcatggtt
     1621 atatcattcc tccgataagc atttcaatga tttgattatt tgtgtagaca tactcgctta
     1681 atatttgagt attttctgtt tggatagcaa taaatttatc gagcaataaa tcgaattggt
     1741 cttttttgta agtcgtaagt tgacggtcaa aatcactttc gattaaaact tttagatatt
     1801 tatttcgact ccatttttct cctgttttat tcgtgagttc ttggcaacgt ttgtctattg
     1861 ttgcaacaaa ctttggatca atattccgaa ctaaaatgtc cataaaaaat ctccttttgc
     1921 aattatgtat aatttatgta taccatacaa atactatatt tgtatagctg ttttttgcca
     1981 aaatgctata cataagctac ccacgatggc gaaaaagtga tatcaaattt ttttctgaga
     2041 aacgtgatag gcacagccta tctacccact ttttttagaa agtgggtaca cgtttcctta
     2101 tgctctttaa aattcggggg ggagtgctgc acgtgtaatc gataaccaaa tttaaaaatg
     2161 cacgatcaaa aatttattta gtttttcaaa cgacattttt ttgagagtat agacggcaaa
     2221 gaagtgattt tatttctaga aaaattgtga gactggagaa agaaaaaatg gaaaaatttc
     2281 cttacgaata ttttcaaaac ttagaaaaat tagagcaagc aattcaagca atcaagttcg
     2341 atttaacaga cgaaaaattg ttggaatata aaaatatgac acccgaaaga aaacgatcgt
     2401 tggaaaaatt aatgcaacta gtgaacgaag acggcggaat ttcagacgca gcttttgacg
     2461 catggagcga atttcatgga gaatataatt aagtgggttt aaatggctct aaaatcgctt
     2521 ctaagagttt tttagcttgg accaatgaaa tacaccaaaa actaaataaa acgtcttaaa
     2581 tcgaaaaata cgagctctca gagcgacttt atttgtaaat gttgcatata agaaataatt
     2641 acggatttta gacttttgta ggatattttt gaaataaata tttatttctg ttataatttg
     2701 attgcaaata agtgaacacg aaacacacga aagccccaga gaggtcggat tctctggggc
     2761 tttttttgtg ggctgagtcg ccttaattaa gttttacttc ttgtttcggt tattcaacca
     2821 gtaactgaac aaagcgctta aaagcccaac aagaagggag caaataaagt tagtgaacac
     2881 gaaacacacc tccttccagt cgcaagactg tcggataggc gacaatcaat tatacaataa
     2941 aaaagcaaaa ataagcctaa gttttttatt gccttggggt tgttagggct tgccctgacc
     3001 gcctgtaagg cgctttagaa cgaaggacat agtttttatc gaatcttgtc ttagaacggc
     3061 ttagacgagc tctcaggacg ccacaaagga gtccctaatg aattattttt ggcgaaggag
     3121 cctaaaggcg actgctgcaa gtaaaatgtg agagcgtagc gatcactcct tcttacagcg
     3181 accataggga gctagattgg cagtttaata tcatgcgcga acctgtttat tttaaaaagt
     3241 gggtgatttt ttggcgggaa attttgctcg cgcgcgcgcg tttattttat ttatttttta
     3301 aagatttttt agaacccttt ttagggggca gttgagcctt actctcccaa cggatacaga
     3361 agggtactaa caacttttta tctgagtact aacaactttt tatctgagta ctaacaactt
     3421 tttatctgag tactaacaac tttttatctg agtactaata tccttttgta taataacaac
     3481 caacctattt acgagttggt tgttaaaggt tattatttat ttgtaagggg ggaagaaagg
     3541 aatggatgaa aaaacggaa
//
LOCUS       DACCXO010000169         3530 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.176, whole genome shotgun sequence.
ACCESSION   DACCXO010000169 DACCXO010000000
VERSION     DACCXO010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.176"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..2303)
                     /locus_tag="IVY70_002724"
     CDS             complement(<1..2303)
                     /locus_tag="IVY70_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959812.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
     gene            complement(2975..3243)
                     /locus_tag="IVY70_002725"
                     /pseudo
     CDS             complement(2975..3243)
                     /locus_tag="IVY70_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          985 a          783 c          560 g         1202 t
ORIGIN      
        1 gtttctacca gctgatatgc tcctggtgct aaaccgtcaa ttgccagttt tccatcttct
       61 cctgtagtta aaccggtttg tagcgtttct ccttctcggt tctgtaactc aaaaacagct
      121 ccttgaagga cttcaccact gccttcagca tgtttaatta gttcaacacc acccgtatca
      181 aggatgtttt caatggttaa cgtcacttct tctatggagt attcatttat aaaaacatca
      241 tgtaccgtcg catctaaaag atacccttcc ggtgcttctg tttctcggac ttgatagcta
      301 cctacaggaa gcggttcact taaaaattct ccgttttctg ccgttatacc ttcttgtatc
      361 acttgtgaat cgcttgataa aatttcaaat tttgctcctg ccagcctttg gccattctca
      421 tcagttttaa ttactttaat acgcccatcg attttccttg ttcttacagt actcgtattc
      481 aatcgattgt cactattcac aagcgttgca aagtttaaaa tttgatcgcc gaccgcatac
      541 gcctctacat tgaccttaaa agagagggta aaacttgtcg gaccaccaat cgtattattc
      601 gcactgaaac gaactattcc attattgact tcggcatcaa tgaaagaggg aatatctgat
      661 agaaatgaca caaattccat gccttcttcc aaaacatctt caataacaag cgtatttctt
      721 gttccagaaa acatcgagac atgcagagta tacgttagtt cattttcatt gagattgata
      781 acatttttgt ccacactttt tcttaaagag attgccggaa gattactttc agggattctc
      841 aagcggtagt ctcggctcat tacctgatta ttgtaggtca tactcgctcg attccgagaa
      901 aacccatcct cttgtcttcc ttcattgaac aagtcgacat cggtcacttc aagagcatat
      961 tcaataaaat acgattgacc ctctgctaga ttaccaaagt ccacaacaag tgtattcgtt
     1021 tccttattaa aagatacttt gtccacaaat tcctcggaaa cataatgagt tctaacgttt
     1081 gcttcacctg gattcgttac ttctgcgatc cgaaccccac ctgaaatagg ttctctattt
     1141 ccagtggaac tccatgtagt cggattcggt gccaaagttg ttccttgcgg tagtacatcg
     1201 gtaaccacta caccgtcttt tcgtgatcct tcataattta cctgcaaaat aaatttatta
     1261 ctgttggtca cgtcttgccc gtcaatcagc agattcccaa gatttccttg gctgtcgatt
     1321 tctctagagc catagtataa tttcgaaaaa atgggtcttg atgaaggaat aggcgtaatt
     1381 gaaaattccg tgtttgctgc tcgctcagca taagtaagac tcacttgaaa gtcattattt
     1441 ggctcagtac cgcctgattg aaagaaagca acttcaaacg tgatactgaa tgtatacgac
     1501 tgagcggctt catttaatcc ttcataatca aaggtaaatg gaaaaacaac gtctgtgcct
     1561 tcttggtaaa atgccagtgg ctttaaaggt gaatcatcag cgacctccac tctaatgaac
     1621 tcaattaagc caatcgatgc tctaggaact cgtacgacca tctccccata ttcttcaaga
     1681 atatcgccag cgcctccttg aatgtatccc tttaacgttt gtgtatgcct gctcggcaaa
     1741 gtggggcgct cagcagagat gccgatgtca taattcaaga gctcctcttg tctttcaatc
     1801 tgagattcct gcgttataga tggctcagat gatctggctt cattaaaaga ttcacttaag
     1861 ctttcttctg gtatattaaa agattcactt gtgctttctt ctggtacatt aaaagactca
     1921 cttgtgcttt cttctggtac actgatttct attgagaccg tatttgattg aatatcaccg
     1981 cttcttgcgg tcaattcgta tttacctgaa tgctgggaag aataagtaat cagacgttcg
     2041 taatcattga gttgcttcac ttccccttca atcaaatcct cactcttatc aatgaccaat
     2101 gcatttagca ggtctggtgt tcctctgact cgcaactgga actgctcacc caaagcagcc
     2161 tcctcttgat caacttcgat cgtcagcgaa cgctctggct gttctgctaa agactcagct
     2221 agaaccgtta tgttcccaaa ttgattgatc acaataagca taactagaaa catacttaac
     2281 agtttatgaa atttgttcct cactattact cccccttata attttgacta aacaacaagg
     2341 tacccgataa ttttctaaaa agattcccca actcaaaatg gctgcgttac ctccctcgta
     2401 ttttcggttc aactcaagcg ttgcaatgta agctgtaaac aaagctaatg attgccgatt
     2461 gtcttctgtt gattgattcg atagctttgt atgactttta gttgtccgtg tggcttaacc
     2521 ataattatga tttcacgatt cctcctgcca gtgccttgca caagctggag cgtaacccta
     2581 atagttattt tgttaatgta cttatatact tttatacttt tccattagaa acaatcacta
     2641 aatcgttgat accaaggtca gactctagaa tttcatcagt gagtccctgt attggttaac
     2701 cttcatcaat cccaaaaaaa tatatcaatg cttagcatca aaatgaatat atagtttaga
     2761 atacttggga ttcatcgata actgatcgct tatttggcca tattatttgg cacttggttc
     2821 aactaaacgc atttaggatg ttccttcatt ctcgtcatgg atgttctcaa accgtcatta
     2881 ctaataaact gtccaccgaa tcgatggttc atttcttgca ttttccacgt ccaattactc
     2941 tttttgctct ttatttgggc gtagtttcac tttcttaact tgtatcgtct tccatcaact
     3001 ccttaatcat cttcttagct ttattggctc ggtttccttg taaaaggtat ctacaaatag
     3061 tgacaatctg tattaaatct tcatcatttt ctgtaatgtc aataatttct atattctgta
     3121 ccatatattg caaagggttt cactaagttc ataaccgaag cgaattaaac aacttttgta
     3181 tagaataact accttttcta tttttccatt ggtaattcta tctattaatt ggtttaatcc
     3241 ttttgttgta gttgattcca ctaccaagat gattctctcg aattttaaag cttgaagatg
     3301 tattgctatt tttgctaact ggcgtatttg caactatttt tcccttctat tcttccttca
     3361 ttgattcccc tctaaaatta ctatccataa aaaattgcat aaatgacaat agtatttata
     3421 ttagtcatac tgctttttaa cacagcaaat tccagaaaaa cattcgttac atatttgtga
     3481 atttttccaa aaaaaataac atttttataa aacgacgaaa aataaatcac
//
LOCUS       DACCXO010000170         3408 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.177, whole genome shotgun sequence.
ACCESSION   DACCXO010000170 DACCXO010000000
VERSION     DACCXO010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.177"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            191..1006
                     /locus_tag="IVY70_002726"
     CDS             191..1006
                     /locus_tag="IVY70_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959813.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1132..1389
                     /locus_tag="IVY70_002727"
     CDS             1132..1389
                     /locus_tag="IVY70_002727"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959814.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1641..2213)
                     /locus_tag="IVY70_002728"
     CDS             complement(1641..2213)
                     /locus_tag="IVY70_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7959815.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2229..2834)
                     /locus_tag="IVY70_002729"
     CDS             complement(2229..2834)
                     /locus_tag="IVY70_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP7959816.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            complement(3235..>3408)
                     /locus_tag="IVY70_002730"
     CDS             complement(3235..>3408)
                     /locus_tag="IVY70_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7959817.1"
                     /translation="LLGMALAYSVGSLIKVNVSLDLFTILLAVGVSSVIGLVFSVMPA
                     SEAAKKDLIDILR"
BASE COUNT         1129 a          619 c          516 g         1144 t
ORIGIN      
        1 ttcttaatag tttttgtaat aatttattaa tcgtagttta tataacttat ttgtaagtga
       61 ctagagaatt aaataaaaaa taattactat ctagtacgaa attaattaca taggataata
      121 tattaaggtg ctggactaca agtaaaaaat actgtatgat catgacagat tcaagaaagg
      181 agcaataaga ttggttaaga tagcaggact ttctaaaggt ggaaaaaccg tttcacaact
      241 ttataaacaa aactacagag actggagaca gttcagaaaa aacattaagg attcttactt
      301 tattctacca acagaacttg aaaaatattt accgaataac aaagctatta atttatatct
      361 gtattactgt tttaaggcaa agaatgaagg cggggattcc tggcattcgg tcaataccat
      421 agcagagtct ctaggagtga caactaaaac tataaacacc tggaataaaa ccttagttga
      481 tttaggtatg attgcacgta tagacgataa ccatctcagt aaatcgacaa ttttacttcc
      541 tctctctagt tactataaat tagcccttga tagttcttta aaggatgtta ccgagagcac
      601 tgtccatgaa attgatggca acttggttgc agtttatcat ttgtttgaat ggagaaaaaa
      661 tgaggctgat gaaaattata acgttcctta taacacactc tgttgtgttt ttaaaagaaa
      721 aacacaaaaa gatactatct ataaatttat cttggtccaa gatgagaaaa taagtaattt
      781 cgaattaagc actccttcta gtaagattta tgacgatgca tatagatttg aatgtcaaga
      841 tctagaagcc ctcctttccg caaataatct aaacaatata gaacatgaaa actttgttgt
      901 aaacactaga tttaatttag tttctgaaaa ggactcagat ttgttagatt tgctgataga
      961 gcttactaaa aatctagata agaaagaaaa cattactgta ctataactag catcggtcca
     1021 tcattaagtt tggactatta gaacacttgc tattacagaa aaaggtatgc tagccacgct
     1081 tactatttat tggtatcaaa caagtatctt aatttgaaag gaagatgatt tatggctaat
     1141 ttatatatag agaacaaagg agacttgaat gccattatta ctttaatcgt agttatggta
     1201 aaatcaatag gtaatatcac aataggaatg ctgtttatta tattagcagc agtgctgatt
     1261 tttatgatag ttatttggtt cttaaatgaa agatctaaag acaatcaaca ccgtagggat
     1321 atggagataa aatcaataga gtcaatagat tatttatcga taataacact aaaaaagcca
     1381 cgttcctaaa cgtgacttac gctgaacgat atgtgttgtt tggtagacga acaaatcgtt
     1441 cattataaat atatgatggt atcctaatat agtaagcgtg gctagcatac ctttaattac
     1501 tttacctttt acagttacca tacacatttt ttgagcagaa atcaagtaaa taaaaaaata
     1561 ttatatttca caacatgcta atagactcat atctagaacg cattaagtat ttacctgtct
     1621 ggttcgctga gacttctttt ttaactttct tcaattttct tctttattct aaaaagcgta
     1681 ctgcgactga aaccggttat agcttcaact tccttatacg atttacctga agtaagcagt
     1741 tcatatgcgt gcctcttctt cggtgtgatt gttgctttcg gacgtccttc tctgaaatta
     1801 ggattattct ttcttgcaaa catttttcct tcttgggtac gttccacaat catatcacgt
     1861 tccatttctg ctacactcaa caacgtccgt gtcaccattc gccccatgga cgtattttca
     1921 atcgttccta gattcaaaac cttgatcgtt acgttatcat cgagtaaagg ttcaatgatc
     1981 tctaatgctt ctctcgtgtt acgagcaaat cgatcaagct tcgtaacaac tatttcatct
     2041 ccaggcttca gaccagccaa cagctgattg aattttggcc gatcccgttt tgtacccgta
     2101 aatttttctg aaaaaatatt cttcttctct acgccgttct caagcaactg ttgaatttgt
     2161 tcttttgtac tttgagaagc ggttgagaca cgtgcatatc catatttcgt catttggtcc
     2221 acctactttt acaaatcatc tatcgtatac tcagaatacc cttcatagaa atagctgaca
     2281 tcgattcctt tcatatataa ctcgcgatcg tcaattttat cagtcagagc attggaaatt
     2341 agatagcgta tttccaaatc gtttatggga cttcgctcca ttgcagaaag atagtctgct
     2401 ttatcgatta agttccaatc tacaactttt tgcaattctt cctttaaaat gagatccagc
     2461 caaattctag tacttcggcc atttccttct cgaaatgggt gagcgatatt catctccaca
     2521 tatttcttga taatctcttc aaaagaagtt tgtggcatct gatcaatatg ctgtaacgaa
     2581 tgttctaaat acataactgg tgcaaatcga aaatttcctt ttgcaatatt gaccgatcgg
     2641 atttttccgg caaaatcgta aatatccgaa aatagaaatt catgaatttc agataaacct
     2701 ttaaatgtac caacttcgat ttcatttatt tttccacaat cgtacaattc tttcgctttt
     2761 tgtttactga gtttttcttc aaccttagcc aactcaactt gattggttaa tcccaattta
     2821 ttctctaaaa tcatgcgaaa tctccttaac tttatgacat aagtttatga aacactctca
     2881 accccattat atcgcgattt tgtttatgtg tcaaaaacta ttaagtttct gacccccgat
     2941 agatttttca agtgtctcta ttttgcacag tctagttgac ctttgataca cctctatatt
     3001 tatagagaat ctaaaacatc aacatggcat actcaaaatt ctaaatatta gggataaaga
     3061 attctcctat acaatcaaaa atttccttta tttcgaactc cggtcaccta actctattga
     3121 tctcttacaa gccaaagaat atctctatac tacaaagaaa caatagggtg cctggttggt
     3181 ctctttgtgt tttattttag aatctagaac gtaaatattc ttgtcattct gattttatct
     3241 aagaatatca attaaatctt tttttgcagc ctctgaagcg ggcattacag aaaagaccag
     3301 tccaataacc gaagataccc caaccgctag taaaatagta aatagatcaa gtgacacgtt
     3361 tacctttatc aaagaaccaa cactataagc aagagccatt cctaataa
//
LOCUS       DACCXO010000171         3404 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.178, whole genome shotgun sequence.
ACCESSION   DACCXO010000171 DACCXO010000000
VERSION     DACCXO010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.178"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..1539
                     /locus_tag="IVY70_002731"
     CDS             <1..1539
                     /locus_tag="IVY70_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959818.1"
                     /translation="FGDYDYSENPNLMPYITEPWVGPLLGNGHTVKDSVKRVITHTKT
                     RTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTYTLSVTMSVGSDWTGETNTIGVR
                     LRYLNEQGGIELPINALIPANVERDKMVTHTFTGITKDNVTSITNCYVEIFSLNSEYK
                     GTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIADPTVVFPHKTSEYMVYGRRNTEN
                     YIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMKPTEGLANTWELTFTVTKQHIDS
                     GVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNISEYKYRGTGMRDSNNPKDYVWD
                     LAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANA
                     NATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            1702..2148
                     /locus_tag="IVY70_002732"
     CDS             1702..2148
                     /locus_tag="IVY70_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959819.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            2150..2287
                     /locus_tag="IVY70_002733"
     CDS             2150..2287
                     /locus_tag="IVY70_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP7959820.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            2325..2618
                     /locus_tag="IVY70_002734"
     CDS             2325..2618
                     /locus_tag="IVY70_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959821.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIRPIVASFTKITKTLSQVSY
                     NLELLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            2615..2839
                     /locus_tag="IVY70_002735"
     CDS             2615..2839
                     /locus_tag="IVY70_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP7959822.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2836..>3404
                     /locus_tag="IVY70_002736"
     CDS             2836..>3404
                     /locus_tag="IVY70_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7959823.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLA"
BASE COUNT         1202 a          563 c          706 g          933 t
ORIGIN      
        1 ttcggggact atgattattc ggagaacccg aatttaatgc catatattac agaaccttgg
       61 gttggacctt tgttaggtaa tggacataca gttaaagata gtgttaaaag ggttataact
      121 catactaaaa ctagaacagc taattccggc gacatattat cgcttggctt aggtattccg
      181 tgtacagcgg aagctaataa taggtatctt ataaccactt tacgaccatc tacgacatac
      241 acattaagcg ttactatgag tgtcggttca gattggactg gagagaccaa tactataggg
      301 gttaggctta gatatttaaa tgaacaaggt ggtatagaac tgcccattaa tgcattaata
      361 ccagcaaatg tagaacgcga taaaatggtc actcatacat ttactggtat aacaaaagat
      421 aatgtaacta gcataacaaa ctgttatgtt gaaatattct cacttaatag tgaatataaa
      481 ggaacggtta gtgtcagcta tgacgttaag ttaaaagcac actaccctaa cttactagat
      541 ggcccttact ggttaggtaa agttccttta ggcgagaata ttgctgaccc tacagttgtc
      601 tttccacaca aaactagtga atatatggtt tatggtagac gcaatacaga aaactatatc
      661 gctgaccaaa catataccat atcaatgaaa gcaactaaac taacagttca atcgtttgcc
      721 gtgtatattg ctgctggccg cgttaaagta ggagacatga agcctactga ggggttagct
      781 aatacatggg agttaacttt tactgtaact aagcaacata tagatagtgg ggtaactaat
      841 tatctggaaa tataccaata cccgtcagct acaaaaggag ctgttcaaat tgagtggctc
      901 aaactagaaa aaggcaatac tcgaaccccg aatattagtg agtataaata tcgtggtact
      961 ggtatgcgtg attcaaacaa tccaaaagat tatgtttggg atctagcacc agaatatgtc
     1021 gaagataatt tggccacaga tattaaaatt tctgaaatta ctggtaaagc aaacaattat
     1081 accgatggga aagtatcgga gattaattcg cagttgactg cttcaattaa tgaagtagac
     1141 accacagcta aggatgctca aacaaaagcg aatgctaatg cgactgctat agatgaatta
     1201 gacaataaga tcgatgaacg cattaatgat acagctacta ccacattaac agttacaaac
     1261 gggaataccg gatcagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
     1321 gctttaaacg gaaaaagcag tggtggaaat gattcaacga tactaacaat tccagaaggc
     1381 tatcggccac caattagttt tgagcaactg gttggctcga tagaccgttc tactttgaac
     1441 agtgctcagt tatctattgg tgcagatgga gccattaaat ggcgaagaaa ctcaagttat
     1501 ggatcggatt atacctttgc aattacttac acgatttaga aaagcgtgaa tcgatatgaa
     1561 ggcagcatat agaccaattg aaccttacgg attcgagcaa atcattgtga atgatgaaga
     1621 acatttaccg gaagaatgca cagaagtcga accaccgatt ccaaattgga aaccgaaatt
     1681 caattactgg gagggaaata aatgaaaaac atttggaaat atggacgtac tggcggagag
     1741 tacgcaggaa aagtattgga cgacatgctt gtatccgttc cttacacaga tcagccaccg
     1801 cttgaaggaa ttcgtgctga tggcgaaccg ttaacaatcg ctgatcaaat gtttgatcct
     1861 aaattgaatc aatggattat tttagcgaac gcactagatc acaacgattt aaacaatctc
     1921 aaagcaatgt atgagtcgtt agaaaatgag aacggcgatt taaaacagat caatgccaaa
     1981 ctcatgctaa gcgatgtagc aattaaacag gaaaatactg cattgaaaga aaaagcggat
     2041 agtttagcac aaatcaattc aaaaatgatg cttgcttcgt tacaaaatag caaagacatt
     2101 tcagaaatta aagagcaact aaatccagct tcaaagggag gtgagtagta tgtttagttt
     2161 tagcgatgtg aaaatgatgt atgattgggg ctgttttact gacgatcaag ttcgactatt
     2221 cgttccacta tgcattacag acgaagaagc agataaaatc attagcaaag aagagagcgc
     2281 atcttaattg atgtgctttt tattttgatt caaggagttg tcacatgatt aatttagggg
     2341 aatggggagc gatagcagga tcaataaccg ctatcgtttc tttgatttta ttagtaatta
     2401 gaccgattgt tgcatcattt acaaaaatta cgaaaacact atcacaagtg agttacaact
     2461 tggaactatt aacaaaagat ttagaatcga gcaaatcaga tcgattgatg attcatgaag
     2521 aactaaagaa acacgatgaa agattagata cacatgcaga aaaattggta gaacacacac
     2581 aacaaattaa aactttattt agggagagaa gaaaatgaat aataaaacgt tcgaagtact
     2641 aaaatggttc gcactggtaa ttattcccgc actagctact ttcgtggggt tagttggtaa
     2701 agcgctcaat tggcagtaca cagatatctg tgttgtcatc attactggtt ttggcgcgtt
     2761 tttagggagt gtgttgggtg tatcaaatcg aacctacaaa atgttctcgg ctgaaagcga
     2821 agaaggagga aacaaatgaa aaagaaaatt actattactg cgatgagcct gttaacggct
     2881 ctttttttat tacccattaa tacgtttgct tatactatta atgacgagta taatttagcg
     2941 ccgaatcaag gagactccag attagcaatt cctaacaaaa ttattttgca cgaaactgga
     3001 atagatgcac cagcaagaaa cgtagccgcc aacatgaaaa ataattataa cggaagcaat
     3061 tcttatacta cagatgttat tggtgacggt gggattgttt accgtgtggg tgagcaagga
     3121 tatgtttcgt ggggagctgg taacgctaat ccttatgcgc ctgtacagat tgaattacag
     3181 cgcacatatg ataaagcatt gtttgaaaaa aactatcgag cttatattga atatacaaga
     3241 gatagtgcaa aaaaatatgg aattccattg actcttgatc aaggaacttc tttatttaca
     3301 aaaggaatca tttctcattt gtgggtgaca aattatgttt gggggaacca cacagatcca
     3361 tatggttact tatcgcaaat gggagttagc aaagaaaagc ttgc
//
LOCUS       DACCXO010000172         3323 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.179, whole genome shotgun sequence.
ACCESSION   DACCXO010000172 DACCXO010000000
VERSION     DACCXO010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.179"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            582..900
                     /locus_tag="IVY70_002737"
                     /pseudo
     CDS             582..900
                     /locus_tag="IVY70_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348881.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            919..1233
                     /locus_tag="IVY70_002738"
     CDS             919..1233
                     /locus_tag="IVY70_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959824.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(1286..2059)
                     /locus_tag="IVY70_002739"
     CDS             complement(1286..2059)
                     /locus_tag="IVY70_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959825.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            2164..2409
                     /locus_tag="IVY70_002740"
     CDS             2164..2409
                     /locus_tag="IVY70_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7959826.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            2565..>3323
                     /locus_tag="IVY70_002741"
     CDS             2565..>3323
                     /locus_tag="IVY70_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959827.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKAD"
BASE COUNT         1236 a          452 c          559 g         1076 t
ORIGIN      
        1 taaagagaaa gttggagcgg aatataaaga aaagagaaaa gtaattaaac gcaaaaaaat
       61 gtagaactta aaaaatataa agaggaacaa ccgttaaaat taggaatgat gttgtgtaaa
      121 cattataatt gctatgatat aaatgaagtg ttaaagaaag caaatttaaa ccaattttgt
      181 gaacagaaaa gccttagtca ctatgaccaa aactacggac gaaactatga ccaaaagtag
      241 gtactaaatt atgaccgaaa ataggtccaa aatggatttt tttatgatat caaaaattat
      301 gaaatgattt tgaaagaaaa ccagttggca cagccaactt agccaccttc ctgtaggaag
      361 gtgctatcag ttttccctta tgctcttttc tttctttttt gtttcatatg tcaaatgcca
      421 ttttgaaatg taccatctta agaaatttat ccttaaaaac aattgcgtta ttggataaaa
      481 agatgctaca ttatcttaaa aaaggaatca aatatgatga atttagtggc tagcatatcg
      541 tgtgattaag atactttata gtgtaagttg aaggagaaaa aatatttaaa atatacttcc
      601 catttcagtg ggaagtaaaa ttaatcttgt tgtttctact agtaatgtat ctttttggaa
      661 actatgtaac gatgtaaaca agattttttt cagaagaaaa aacgataaaa gttgtgataa
      721 acgatttaaa aaataggaga aaatatatca agcgcatggt atgaaacgca catttaaaaa
      781 gattcgacta actaaaaaaa taagtttaca aagcattgtt gtgtattaca agtgatctat
      841 acactgaaaa atgatagaga aaatctgata cgtccgcaac aatggacaat tgaaaatgaa
      901 agtgaggaaa ttggaagaat ggctgatgaa aataaagttg ttccgcttga aacactagtg
      961 ataaataagg aagagtttga gcaacttttt cttgattttt ttgttcaata tgacaggatt
     1021 tatgatttta gttcgtcact agatttattt gaattagagg cagaaataat agaaaacagt
     1081 cgagaggaga atggtaaaat catcatttca gtagaaaaat tcaatgaatt attctcagaa
     1141 acatttgaaa tagatacaga ttattatcaa ccacaagaag ttttccaatc atttagaaat
     1201 cagattattc gctcaggtaa gaccgtaaac tgaaaaacaa taaaagaaac gttgaaagaa
     1261 taattattcc ttcaacgttt cttttttaga atgttttaat gaccaaagca gtttcattgt
     1321 caggcttagg taattcatga aatcccgtat ttatagtgct caattgatca actaaccatg
     1381 ttttactttt tgaattgtag ataaatctaa aattaatatc gttattatcg tgttgaaggg
     1441 gcgtctccaa ataatctgct gtatattcat agtcaacgta acctgctaat acaaaagaga
     1501 gtatcccttt atttaatgtt agatttaatg gataatcata cgactttatt tttttcaact
     1561 tcatattgta catatcttca gccataaaag ttgagttcgt gatttcatct tgggtctcat
     1621 taaaaaagtt atccgaataa tcgcttattt ttgaaaagtc tttattggca ccagcctcaa
     1681 aaaattgttc aactttttca gtataaagtt gtgctaacgc tcctttttca atattaactt
     1741 taatattttc atgtctatca atttgtgtgc tgttcagtaa gggaagatca gcttttagtt
     1801 ttatatcgtc ttgatttctt tcaatttcaa ttggcaaaat cttttcagta gattcagttt
     1861 ctgataaagg aacatctagt tttttatcat gataataagc ttcaaatcca tctggaaccg
     1921 gaaaaaaatg tattgggtaa ttcttttctg aggttgttgt actagtttcc gtacttgtag
     1981 aacttgtgtc agaagtggta cacccgctta tagttccgat gagtaaagcg caagttagaa
     2041 tgattgcaaa tttcttcaaa aaaattcctc cttaaaattg aatatggact aatactagtc
     2101 ccattgtagc ataccgaatc tttaagatta ataagaaaaa ggactaattt tagtccaaaa
     2161 ggtgtgctat tcttgaaaga ggaaaaaatt attgataaag aaaatgtaat cggtagcaat
     2221 attagacgag tccgattaga aaaaggaatt ggtcaaaccg agctgattag agacctacag
     2281 cttcgtaaaa ttgctataac tagggaaacg ttggttaaga ttgaaggtgg ccgccaacac
     2341 attaagttgg atcaattgaa agccatcaaa gatattctac aggtgtctta tgaagatttg
     2401 cttcaatgac tacttgtagt catttcgttt gaaattatta tgttacaccc ttatgtgtca
     2461 cgaagaaata ctggtataat aatgaatggg atatgtaacg atagggtata ctgttatgtt
     2521 gcctaataaa ttgtggatca aaatacaaga atggagggat atccttgtat aaggcatatg
     2581 aattggattt tacaaatgct gacttaaatg cattctcctc aagctcgcaa tcatatacac
     2641 atgtagtaaa tcaaatagat cacaaccaaa aatctattaa caagagaata gaaaatctat
     2701 ttgaaagtga aaatgattta gttgattatt attctaagga cagcaaaatt ctagatgcag
     2761 acgaaattat taaagattgg tttccaacta taaaagcaga tatttttata tctcactctc
     2821 atgctgatga aaaattagct atacgttttg catcatggct atttgaaaat tttggtctta
     2881 ctgcatttat tgattcttcg gtttggggat acagttctga tttattaaaa aaaatagacc
     2941 aaaaatattg ctataaggaa caaacaaaaa catatgatta tgataaaaga aatgtaacaa
     3001 ctagtcatgt tcatatgatg ctttctacag ctttaaataa tatgattgat agtacagaat
     3061 gtctgttttt tcttaataca cctaattcaa ttagtttgag caatgaaatc actaatgaac
     3121 agaaatttac gtattcgcca tggctatata gcgaactgac gacagcttca atagtggaaa
     3181 aaaagaatcc acgattggaa agtaatcctc aaatgagcac cgaggatgtt agaagcatta
     3241 taaagcacta ttcttcgcaa gaaaatctgc aaataaaata ttctttaaat ttaagtgaat
     3301 taacttattt acgaaaagct gat
//
LOCUS       DACCXO010000173         3245 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.180, whole genome shotgun sequence.
ACCESSION   DACCXO010000173 DACCXO010000000
VERSION     DACCXO010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.180"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..206
                     /locus_tag="IVY70_002742"
     CDS             <1..206
                     /locus_tag="IVY70_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7959828.1"
                     /translation="KVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGY
                     RNQRLAQRILDNELGCANASEAV"
     gene            341..562
                     /locus_tag="IVY70_002743"
                     /pseudo
     CDS             341..562
                     /locus_tag="IVY70_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            562..909
                     /locus_tag="IVY70_002744"
     CDS             562..909
                     /locus_tag="IVY70_002744"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959829.1"
                     /translation="MRKRTIKTQLAVSFLAIATLIIGSISLVALSLMNNHFSKYVEER
                     QEDLLNQYVYTIDLLWLNSGETWNSEELAALSEKVLENNIYFSIEDEQGNMVWELTGK
                     DLKSAQEKLKKMH"
     gene            906..1955
                     /locus_tag="IVY70_002745"
     CDS             906..1955
                     /locus_tag="IVY70_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7959830.1"
                     /translation="MKVSEKNSVKLDETIEVKKKLINDGNEFGKVTFYYFGPFAYTEH
                     DALFISSMKQSLMYVAIAALLVSFILASWISARLGLPLKHVSDFTHKLTRGEYADKIP
                     QETSIIEINSLIDSLNDLSNQLEKQHGLRKRLTTDISHELRTPLATLKGNVEGMIDGV
                     WKITPERLQSCYDEIDRLTRLIGNIEIINKIEAKYDHLNKTEFNIYKLIESVIENFAS
                     KIESKNLHVEIQGDNINISADKDKMNQVVINLLNNAIKFTQKEGTIKFSISKNKDHVL
                     LIVEDNGIGIEKDQQLHIFDRFYMADPSRSRALGGQGIGLAIVKSVIEAHKGSITVKS
                     KLGLGTKFVIKLPFQ"
     gene            1970..2176
                     /locus_tag="IVY70_002746"
     CDS             1970..2176
                     /locus_tag="IVY70_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959831.1"
                     /translation="MFQKQIKIAKLVCECWAKKIFFQLISIDSVEKVHLDILSNSVVI
                     FSKKDLSIATIKKEVFGLQIYEMT"
     gene            2197..2793
                     /locus_tag="IVY70_002747"
     CDS             2197..2793
                     /locus_tag="IVY70_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhK family protein"
                     /protein_id="HAP7959832.1"
                     /translation="MKTVKKMTKLVFASLGIIVFLGACSNQSESNNSNSTNEESTSTA
                     SSEMNSMEGMNHEGMVPSSMKDAANPKFPVGSNVILLGDHMEGMKGANAQVVGAFDTT
                     MYEVSYEPKTGGPMVNNHRWVVQEELKDTETVANEGDTVILNADHMDGMMGAEAKVDK
                     SITGTVYVVNYTPTDGQEEVKNHMWVTEDEMEYDENNE"
BASE COUNT         1158 a          467 c          650 g          970 t
ORIGIN      
        1 tgaaagtaga atttgtctat caatacacat ttgaaacctt acaacaattg gatttggagt
       61 tatttgacta tgtcaattgg tggaaccacc ttcggttgca cggtacactt ggctacgaga
      121 caccggttgg ttaccgtaac cagagattgg cgcagcgaat ccttgataat gagctcggat
      181 gtgctaacgc tagcgaggca gtctaacttt aactgttagc tcctgccgaa gatcgtcaca
      241 tccgaggagg ctcattgtca aggacaatcg gaatagcata cggaaagaag ttctgcacct
      301 tataaaattt gtcaaaaaaa ctgttgccat tccacttaaa acaactgaat ttgatattct
      361 acacgctcta atgtccacac ctaataaagt cttttctcgt agtgatttga tagagaaagt
      421 taagggaata gaatttgatg gactagatag gagcatcgat tctcatataa aaaacttaag
      481 acataaaatt gagaatgatg ctaaatcacc tatgtatatt ttgacagtcc atggtacagg
      541 atacagattt ggtggccact aatgagaaaa agaacgataa aaactcaatt agctgtttcc
      601 tttttagcaa tcgcaacatt aataatagga tcgattagtt tagtcgcatt aagtttgatg
      661 aacaatcatt ttagtaaata cgtggaagag cggcaagaag atttgttgaa tcagtatgta
      721 tatacaattg atttattgtg gttaaatagt ggggaaacat ggaatagtga agaacttgca
      781 gcactatctg agaaagtatt agagaataat atttattttt ctatagaaga cgaacaaggc
      841 aacatggttt gggaacttac tggaaaagat ttgaagtcag cacaggagaa actgaaaaaa
      901 atgcattgaa agtttctgag aaaaacagtg tgaaactaga cgaaacaata gaagtgaaaa
      961 agaagttaat taatgatgga aacgagtttg gaaaagtaac gttttattat ttcggacctt
     1021 ttgcttatac agagcacgat gccttgttta tctccagtat gaaacaaagt ttaatgtatg
     1081 ttgcaattgc agctcttctt gtatcattca tacttgcttc gtggatctct gctagattag
     1141 gattgccact aaagcatgta agcgatttta cacataaatt aactcgtggg gaatatgctg
     1201 ataaaatccc acaagaaact tcaattattg aaataaattc cttaattgat tccctaaatg
     1261 atttaagtaa tcagttggag aaacagcatg ggcttcgaaa aagattaact accgatattt
     1321 cacatgaatt aagaactcca ttagctacgc ttaaaggcaa tgtggaagga atgattgacg
     1381 gagtctggaa aataactccc gagcgcctac aatcttgtta tgacgaaata gatcgtctaa
     1441 ctcgtttaat tggaaatata gaaataatca acaaaattga agcaaaatat gatcatttaa
     1501 ataaaactga gtttaacata tataagttga ttgaatccgt tattgaaaat ttcgccagca
     1561 aaattgagtc aaaaaacctt catgttgaaa tccaagggga taacatcaat atatctgcag
     1621 ataaagataa aatgaaccaa gttgttatca atcttttgaa taatgctatt aaatttacgc
     1681 aaaaggaggg aaccattaag ttttctataa gtaaaaataa agaccatgtt cttttaattg
     1741 ttgaagacaa cggcatcggt atagaaaaag atcagcaact tcatattttt gatagatttt
     1801 atatggctga cccctccaga agtagagctt taggggggca aggtatcgga ttagctattg
     1861 taaagagcgt tattgaagcg cataaaggta gtataactgt aaaaagtaaa ttaggtctag
     1921 gaactaaatt tgttataaag ttaccatttc aataaaggag gagagttatt tgtttcaaaa
     1981 acagataaaa attgctaaac tagtatgtga atgttgggca aaaaaaatat tttttcaact
     2041 gatttcgata gatagtgtcg aaaaagttca tttagatatc ttatcaaatt ctgtagtaat
     2101 cttttctaag aaggacttat caatagctac aattaaaaaa gaagtttttg gattacaaat
     2161 ttatgagatg acctaaatgt gattaattaa aggaggatga agacagtgaa aaagatgaca
     2221 aaattagtat ttgcaagtct tggaattatt gtatttttag gagcgtgtag caatcaaagt
     2281 gaatcgaata attctaatag cacaaacgag gaatcgactt ctacagcgtc tagtgaaatg
     2341 aatagtatgg agggaatgaa tcacgaaggt atggttcctt cttcaatgaa agatgctgcg
     2401 aaccctaaat tcccggtggg aagtaatgtt atactgctag gcgatcatat ggaagggatg
     2461 aaaggtgcaa atgctcaagt tgtaggggcc tttgatacta caatgtatga agtaagctat
     2521 gagccaaaaa ctgggggacc aatggttaac aatcatcgat gggtagtaca agaagagtta
     2581 aaagatactg agactgtagc aaatgagggc gatactgtta tcttgaatgc cgatcacatg
     2641 gatgggatga tgggagctga agcaaaagta gataaatcta ttacaggaac tgtttacgtt
     2701 gttaattaca ctccaaccga tggtcaagaa gaagttaaga atcatatgtg ggtaactgaa
     2761 gatgagatgg aatatgacga aaataatgag taattttata tactaaacca acaagctggt
     2821 acatggtctt cgtattctca acatgaggaa tatgagctct ttgttaataa ggactgatga
     2881 gttgtgcaaa atcaaatctg ggtcagaacg aacctcattc tggctcagat ttttggctct
     2941 tcgacattta gtgctggtag agagacaact gaaaatttag tcataaccag ttaggcagag
     3001 tgcttaactg gtttttgttc gcatttatta ttcttttgat attggctgct tgcccttcaa
     3061 ttttatttgg tttcttaatc aactcttcat ataaccccaa atcatatatt aatgaggtgc
     3121 ctcctattcc tataaatcta tctcttcttt aatagtatga gaaaagtcta ctcttgtaga
     3181 tttaaattgt caacaaaaaa tttacagatg taaatgataa gttgaataga gaaagataaa
     3241 ttatt
//
LOCUS       DACCXO010000174         3240 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.181, whole genome shotgun sequence.
ACCESSION   DACCXO010000174 DACCXO010000000
VERSION     DACCXO010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.181"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(551..2164)
                     /locus_tag="IVY70_002748"
     CDS             complement(551..2164)
                     /locus_tag="IVY70_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP7959833.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2305..3213)
                     /locus_tag="IVY70_002749"
     CDS             complement(2305..3213)
                     /locus_tag="IVY70_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7959834.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1045 a          557 c          572 g         1066 t
ORIGIN      
        1 taaataaata ggaacaaaaa aattaaatta tttaatacac acattaggga tcattcattg
       61 aaggattaga agacaacgag gatggcgaaa aatatgatta caatagaaaa gtcgttctaa
      121 accaatcagt tcctagtatc acacaacgca agccagtcga tttggtgaga ttagaggagt
      181 gaaagcaaac tttattatat aagaaaaaat attcgaaagg gatgctagtt taggtactag
      241 tgctgataga ttaatatgat gcctcgtaat attaggcgta gttactctag agacatcact
      301 atggactatg atttgtctca gagtcacaac atgttttttt atgaaaagaa acagctaaaa
      361 taattaaatt gcgcaatgtt taaactttct taaactataa cctaataaat gtgaagaaat
      421 aatgcacaat ttcttcacat ttatcttcta ttctatatac ataccaacca acaaccctat
      481 atactttttc atttttactc ctcaagtaaa aataactcct agaccgcggc tacccaccgc
      541 ggtcgttttt ttattcttca atcaaaatcg ctttgatacc caaacctgcc aagattcggg
      601 cagaagtata agtattcaat cctcttctga aagtaatgta aactttttta tcttttggga
      661 tttcattgat tcgatctcgt aactcattca ttggaatatt ttttgttgcc gagatactgc
      721 cagaaggtgc tttattaggt tcacgtatat ctaaaaagaa cgcttctttc aaatcgtttt
      781 cgggtatatc acttgctttg atcgtttcta cgatattcgt catttgattg attgcgacat
      841 aaccagctat attcaacggg tcacgggtag tagaatacgg tggagagtaa ggcaactcca
      901 aatcaggcaa atcaaagact gtcaagtttc cggtgattgc tacagaaagt tctcccatcc
      961 gtttatcgac acctttttct ccaaatgctt gtccacctaa aatacgaccg ctatctgcat
     1021 caaaaatcag ctttagattt aatcttgtag cttctggata gaaataggca tggtcaaagg
     1081 gagtgataaa aacagtttta taatttgtta tgcctgatgc ttttaattca tgttctgtca
     1141 ttcccgcata acttgctgta tgatcaaaga tttttgcgac acctgcacca atatatccgc
     1201 gatagcgcat aggcgtacca tttaaaatat ctgccagcat atgtccttga cgattggccg
     1261 cactggaaag catactttgg atcggctgac cagtcactac actagtcgtt tcgataatat
     1321 cgccaatcgc atagatgtcc ggcagattgg tttgtaactg gtcatcaaca atgatctgac
     1381 ctgtatctga taattgtatg ccggttgctt tcaccacttc attcttcgga gaaacaccaa
     1441 cagcaaaaat tagcatatca gcagaaagga cggagccatc tgataatact atttctcgac
     1501 ctttatcacg gatctcagta actcttgttt ctaaatgaac ggctaatcct tctttcagca
     1561 atttgtcata aactaaatta gcaatttctg gatcataagg gaaagcgact tgtggcaatt
     1621 gatcgattaa agtcacgtct aagccgcgat gcttgaaatt ctcagctaat tccagaccca
     1681 taacacctgc accaagaatg acgacatgtt gtggattttt ttcttcaagg aacgatttga
     1741 ttcgatccgc atccgtcaca ctacggagga caaagccgtt ttctgcttca aaaactccgg
     1801 gaatatccgg ataatctggt ctagcaccag aagagatgat caacttatcg taatctgttt
     1861 ttatttcttc atttgtggat aggtctttaa ctgttaatcg cttagtagaa ggatcgattg
     1921 ctgttacttc gtgtttagta aagacatcga tgttgttttt tgttttcaat atttctggtg
     1981 tacgttcgat cagcgagtca cggtcagtga tcacacctcc taaataataa ggcaaagcac
     2041 aacttgcata agaaatattt tctccgcgtt catagataat gatttcgtgc gcttcattca
     2101 atcgacggaa acgagtggca aatgaaggac cgccagctac accgccaatg acaacaactt
     2161 tcataaaaac atctcctttt ctacgttgat cgtactatgt tatggtaaaa aacagcaaat
     2221 aattgtgccg gaaaattatt gtttattttg aatagacaga aaatatagag aaataatttg
     2281 tggataaaac ctaaaaattt ttttctattt taaggataga taatactttg aaggggataa
     2341 ccctgtatat tttttaaaag ctgaagaaaa atgatggatc gaatgaaatc ctaatatatc
     2401 tgcaatttct gtataggtta attcattttc tagcacgaga cgttttgctt tttccatttt
     2461 tagttcttta aaatagctaa taacactttt tccattcact tttctaaaat gtttttttaa
     2521 gctggctgga ctgataaaaa aaatttgggc taattgttca agcaaaaaat tttctgaaag
     2581 atgttcttct aaatatcgta tgattggatc aactaattca tctgaataaa gaatatgctt
     2641 ttttttagga atgactttct catttgaagg tattgtcaat aaagcaagtt cctgttgata
     2701 atgccttggt aaggaagaca attgaatttt ttgcagacaa taagtaacta ataaggaaag
     2761 caaatgaatg ctcatctgtt ggcatagtat gaaaaaagaa ttgatttcct gatcatagga
     2821 aatagctggc ttactatgtt ttagataatc aattttgaac tgttcatttt tcagaaaagt
     2881 aatgatagat gttatttctt gatctataga aaaagattgt cccgtaatat gtgctaataa
     2941 ttgctcatca aaataaaact ggataatgat aaaagtttgt gtttcctttt gagtagaaaa
     3001 gatttggact tcttgctgtt tatcaatgaa taaaagggaa ggattatcta gttctataga
     3061 tttatgtgca aattcagcat ataaattagc gctttcattt attagtagtc gaaaataggg
     3121 agcagcagat aaatgaagat gggtatctat ggaataggtt agtgtttttt tgacaagatt
     3181 ggaggattgg ttgtttgtta acaaaagaac cactttcctt tctcttttta tataaaaagc
//
LOCUS       DACCXO010000175         3163 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.182, whole genome shotgun sequence.
ACCESSION   DACCXO010000175 DACCXO010000000
VERSION     DACCXO010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.182"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            429..1652
                     /locus_tag="IVY70_002750"
     CDS             429..1652
                     /locus_tag="IVY70_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP7959835.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLEDHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            1687..2454
                     /locus_tag="IVY70_002751"
     CDS             1687..2454
                     /locus_tag="IVY70_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP7959836.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFKDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LKIDFAKVRKGRNYNFAVQFNISSEFDEKPYREKKLG"
     gene            2644..3042
                     /locus_tag="IVY70_002752"
     CDS             2644..3042
                     /locus_tag="IVY70_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP7959837.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT         1189 a          434 c          537 g         1003 t
ORIGIN      
        1 atattttaat ctttaaaact ttagcaacct ccaaaaaggc tttatatcaa tcttaaatag
       61 attttagggt ggacataaag cgatgcgaac atggacaaaa agcgatgtga atgtggacaa
      121 aaagcgatgc aaacgtggac aaaaagcgat gtggtattcc gaatatatcc aaaaaaagaa
      181 aaagtatgtg aatttatttc taaaaactag aaaaatattt gttttttaat tttggtaatt
      241 tttataattt cgtattttat tgaaaaggat ttttattttg aaaatgttct aaaatagcga
      301 tttctgctgt attttcaatg aaatttgatt ggaaaaaata aaatgtgttt tactgacatt
      361 gagttatgtt ataaatttac gatacatttc ttattgtaac gtatcgaaaa caggtctata
      421 cttattcaat ggaatcgtac cagttccaag tttttaagct cgtaccaagc ttagaaagaa
      481 gaggcaatcg tatgaaaata acagaaaaga atgtaaaccc gaaatttgaa actaccgaaa
      541 gcaatctaaa aaagtttgat ttaactgtta ctttatcgaa tcaacttgta gatagcattg
      601 ttgaaatgga tgctatgcca caaaaaattt atctttacgc tataggacat cttgacccta
      661 aaaatccgcc ttttaataat accgtcaaag tatctaaaaa ggctttattt gcgttgatgt
      721 cttctagttc atcttcttca aaacattcaa gattaaaaca agttattgaa aaattgcatc
      781 agcaatctgt cattaaaatt caaagaacta aacctaacgg taagactgaa tacaaagtca
      841 tctcaccgct agaaattact gaattcaatg atttttctga tgcagtatct attaaattta
      901 gtagttcaat tatgccttta ttaacgaact tagaagatca ttttacgcaa tatacatttg
      961 atcaagtagt taacttgaca agcaagtatg cgatcagttt ttttcaatta ttcactaaag
     1021 attttaatct gtatgaagca aatagagata acttaacatg gagtacaaaa tcacttgata
     1081 gattaaaaaa tcctgttttc tccattgcag atttacgaaa gaaatttagt attgagaaaa
     1141 aatataaagc ttttcgagac tttgaaagac gtgtaattaa agaaccgatt gcccaactga
     1201 ataaatacac tgattttaaa attgactatg agcttatcaa gaagggtcat acttatacaa
     1261 acataaagtt cttgcttaca cgtaccatgt cagttccggt accttataag caagaacaaa
     1321 gagaccctgc ttatttaaaa ggcgcagaag ataaaacaga gaaaatgaat caatcgtact
     1381 tgaaagctat gaatagcaaa tacactaaat tactaatcaa aaactttttg atcagttctg
     1441 ccgacttatg cgatcttaaa cttatgagcc gacttcagaa taaagtttat ccgctttatg
     1501 atcgtttatc caaagataga ggatttgaat cagttgtcaa tcacctatct tacgttgcta
     1561 ctcataagga ggattacaca ataaaaaata ctgttaaata tctcgaaaaa tcaattacga
     1621 gttatttgcc ttatgtgaac ctttatgatt aacaactgaa aaaatgcgtg aaggaaggaa
     1681 aaacttttga aaaaagatga actgacaaag tacaatgagg agttcttagc aacaattgat
     1741 atgaaaatgg atttaaagtc gtatgaactt tatcaattgc tgttcagtta tatctcaccc
     1801 acaaagctaa agaaaacagg cgtagtagat ttacctgtta aagcgattat taattacatg
     1861 gatgttgatc aaaatcttga aacggaacta aaaaccatag ttaacacttt gcaaagacaa
     1921 gcagtttatg aaatatcgaa tctagacaag aaatataaga cgtatactac tgtatctgct
     1981 attgatcgga ttcattggga tttagaagag catagtgtat ctttcagttt ttcagaagat
     2041 gtaattccct taattgtctc aatcaaaaag aagtatccgt tctttgtttt taaagactta
     2101 gcatcttttc aaaaaaaata tagcatcgtg ttatacaaat atttggcgat gtattataaa
     2161 aaatatctta tcgccaaaaa taaaggagat tttgagaaag ctgaaatata taagaatccc
     2221 ctgattgaaa tagttaaaat tcgtgagatt acgaaaacag aaaatgagta taaaacaata
     2281 tcgaattttg atagacgtgt tttaagggaa tcagttgaag aaattaatga aaaatcaatg
     2341 ctgaagattg attttgctaa agttagaaaa ggcagaaatt acaattttgc tgtccagttt
     2401 aatatttcta gtgagtttga tgaaaaacct tatagagaaa aaaaattagg ttaatgcaaa
     2461 atatatctta ctcgtttgaa gatgcttcag acgtatgtta agatatgtag tgccttggga
     2521 gcaaggtatt aatctccttt agaggttaat tagcttgtga tcgcctatca attttttggt
     2581 aggcgattct ttttttgaaa ccaaaaagca aaaattattt tttaattaga gaggtgcaat
     2641 gcgatgaaat actttttttg tgcagtattc gaatggaaag gaaatgaata tgcagttaaa
     2701 ttcccagatt ttccagactt atatacgcat ggtgaaactt tagaagatgc ttttgagatg
     2761 gcgcaagaag tattggaagg atacttggtg cttttagagt tgcaagaaaa agaaattcca
     2821 tcgcctacgc ctgttcaaga gttaaatgca agaatcaagc gatctgagaa tgttaattta
     2881 tttgtcgtaa aagctaatac gaaagttgct aaagctcgaa acaagcaaaa ttatgatcga
     2941 aaatcagtga ctgtggaaca ctatttaaat gttttagcgg ctgaagagaa tattaatctg
     3001 tcgaaagctt tgaatgattt gttgagagaa aagttgattt gatgtctctc agatgatcat
     3061 attaagcaaa aacagcttgt tttaggacgt atggatttaa gaaagacaaa ttatcaaaga
     3121 atatattaaa agctctcaga agcgttctga gagcttttaa att
//
LOCUS       DACCXO010000176         3080 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.183, whole genome shotgun sequence.
ACCESSION   DACCXO010000176 DACCXO010000000
VERSION     DACCXO010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.183"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(561..1601)
                     /locus_tag="IVY70_002753"
     CDS             complement(561..1601)
                     /locus_tag="IVY70_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP7959838.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1950..2276)
                     /locus_tag="IVY70_002754"
     CDS             complement(1950..2276)
                     /locus_tag="IVY70_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959839.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2263..3066)
                     /locus_tag="IVY70_002755"
     CDS             complement(2263..3066)
                     /locus_tag="IVY70_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7959840.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT          916 a          536 c          433 g         1195 t
ORIGIN      
        1 tccgttgcgg gtaataccgt aacactgaag ggcacgcatt aaggcgtcat cttaatgctt
       61 tcctgtagga cactgacgga gattaccagt ctcccgagcc ttacaggggc ctttcttttt
      121 tttgtatttt caattatcac aatttatcgt ccaaaagtgc agaacgaaaa tcaattcatc
      181 acaagcatat cagacaatta aatttaaaac aactaaaaat atacaaaaaa gtgtaattga
      241 atattagcag cactgcctga aaagaagtaa tatcaggctt ttcagcttgg ggaaataagt
      301 gaattacctc tcttcatgaa ttattataac tcaaaaaagc acatatgcaa acactagttc
      361 cctatttttt tgaatgaaaa ataactaaaa tatctaacga ttatgacttt aaaacctacg
      421 tttgtgattg ttttcataaa cttttaatct aaaaactccg gaataatagc tatttatttg
      481 taacatttgt aataaaatta aaatcataaa aaagttctaa tttaaaattg atcattttta
      541 tagtgatcgc tttttatcaa ctactctagc caattttccg tactaactgt tggtaattcg
      601 ttaaatgttt ctctttgttc gtttgcaagc tttgccgcaa tagctttttc gacaaaccaa
      661 ttttttacat aaacgaaaat tagattctgc aaattgttta ctttttcagt tttttgtttt
      721 tgataagctt tcagcaaagt catatataat cctttgtcag catcaattcc ataagattcc
      781 agttcttcat aaacaacaac tgtttgctct aattcttcag cttttttctt tgcattatga
      841 atagtttgga cggttttttg agcgtcttcg taagtactag aaaatgtttt tatcaagttt
      901 aatacatttt ctggtacaaa tgtatttata ctgtcctctt tcatgatgtc gacgaagtta
      961 tctagtaaaa cttgattttg taactgatca gtttcagtgt ctattatgtg tctattagtg
     1021 tctatagaat tagaatagaa tagattgtct gcgattttcg caattacggt tgagtccttg
     1081 atatgactgg attttgccgt atttgcgatt ttcgcaatta cggtggtatc attgatttct
     1141 cgcggttttg ccgtatttgc gattttcgca attacggtgg tatcagtatt gtcttcctct
     1201 tccatgattt taaagacgtc ttgattcgtt acttctggct tgcctaagta caataaatta
     1261 ggcatggatt caatcttacc gtcacgttta tttactcttc ctctcttctg tttcagaaga
     1321 cctgcttttt ctaattcctt tttgatttta gatactttac cttcgcggca ttgtaacaat
     1381 ttcattaatt cttcaactgt gaatatgaaa aatatattat catcagaatc tacccagtta
     1441 tttttgactg aataatcaaa tcgatcttta agtagcatat aagcaatttt tgcatcattt
     1501 gatagatcca tgtatttttc gttagtaaaa aataccttag gcatttgata atatttttca
     1561 cgataaattt cttttgattt gtaataatta aaatcattca tagccaatcc tctttctgta
     1621 ggatagcgtt ttgttcaaaa ctatgttaca atgaattaaa caaatatatc tatccactat
     1681 atatttttct actgctgata agtcgccaaa ctttgttaaa gcagtagtct ttttttatct
     1741 ttttttagtt taccttaaaa aataaattaa agcaaaccgc tctatttcaa ggtttaagct
     1801 catagtaact atatcacgat attacaataa tattatatca cgatatcgta atattacaat
     1861 atgaagataa gcgacttatc agctttgata ctactgattt ttataaaaaa aatatcacga
     1921 tattatgata tcgtgatatt gaatgtaaat taaattttat tttgtttctt tgctagttta
     1981 tatccttcaa tcaagtattc ttcaggtatg gactttaata gcgcatcttc tataacgtct
     2041 ggtttacttt tgtctgattc agcaatagtc gtccaatatt tgagtgctga cattagaatt
     2101 ggatttatac gaatgctatc tgctgttctt acactacttc cttgatcttt tatgacttta
     2161 cttatatttt tcttggttaa ttcgatttct tctacttctt ttgatttaaa attcttttta
     2221 tttttatttc ctagtaatct acttactgca gcttgactgc gattatcttc tgccatcaaa
     2281 atcaccaact tcttttaaat attcttctgt aaatgcttta tagtcatctt cagctgttga
     2341 gggtttatta aaattgatta gatctgatcc agcagcttgc gcttcgtcaa cggttattgt
     2401 cctacgaata taagttttga aaatttttgc accaatgact ctagaaattt cggttgctgc
     2461 agacgtcatt tctttagaaa tgttataacg cttattatac ttagtaaata gaactcctcg
     2521 tacttcaagc ttgtcgttcg tatattcttt taccgtttca atagtttcac ttaactttat
     2581 tagtcctttt aatgagaaat acgcagcttc aactggaatg ataatttcat cagaaacagt
     2641 aaatgcgttg actgtaagca ttcctaaact tggtggacaa tcaattataa tataatcata
     2701 gttgtccttg attggttgaa gtactctttt aagcctgtgt tcacttccta cttgagtaaa
     2761 ttccctctct gcagaagaaa gggctatgtc ggctggcaaa acatcaattc ctttatagtc
     2821 cagaatagca tctgtaatgt cagcctcccc ttttaatact tcataaattg taggttggtt
     2881 tgttccatct atatcgaaac ctacattatc tgtagcattt ccctgaggat caatgtccac
     2941 taaaagtaca tttttaccgt aaacaaattt caaacatgct gctaaattaa cgctagtcgt
     3001 agttttagaa actccacctt tttgattggc tactgacaat attcttgcca ttaaaatatc
     3061 ccccattcat cttatattat
//
LOCUS       DACCXO010000177         3036 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.184, whole genome shotgun sequence.
ACCESSION   DACCXO010000177 DACCXO010000000
VERSION     DACCXO010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.184"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            41..1705
                     /gene="alsS"
                     /locus_tag="IVY70_002756"
     CDS             41..1705
                     /gene="alsS"
                     /locus_tag="IVY70_002756"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP7959841.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            1718..2428
                     /gene="budA"
                     /locus_tag="IVY70_002757"
     CDS             1718..2428
                     /gene="budA"
                     /locus_tag="IVY70_002757"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP7959842.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            2558..>3036
                     /locus_tag="IVY70_002758"
     CDS             2558..>3036
                     /locus_tag="IVY70_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP7959843.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINA"
BASE COUNT         1015 a          506 c          688 g          827 t
ORIGIN      
        1 actaataatt aaaaataaag cataaaaagg acgatgttca atggacgaaa aaatgaaaca
       61 aggttcgaaa tctgtagtag aaagtttgat taatcatcat gttgattatg tattcggtat
      121 accaggtgca aaaatcgatg gtgtatttaa tgaattagag gatcaaggtc ctgaattaat
      181 cgtgactcgt cacgaacaaa atgctgcatt tatggcacaa gcaatcggac gtattacagg
      241 agagccagga gtagttatcg caacaagtgg tcctggtgct agtaacttag ctacaggttt
      301 ggtaacagcg acagcagaag gtgacccagt tttagcaatt gccggacaag taaaacgaag
      361 cgatctattg aagttgaccc atcaaagcat ggataatgca gcactgttcc agccaatcac
      421 aaaatacagt gcagaaatcc aagatccaga aacaatttca gaagtgattg ccaatgctta
      481 tcgtatggct aagagttcaa aaaaaggcgc gagctttatc agtatcccgc aagatgtagt
      541 ggatgcacct gtccaaggga atgtcatcaa gccattgagc gatcctaaat taggttctgc
      601 ttctgcagat gatatccgtt acctagctga acgcattaga gaagcaaaac taccggtact
      661 attagtaggt atgcgaggat caagtgaaaa agaaacgtta gccatcaggc aattagttgg
      721 aaagacagca ttgcctgttg tagagacatt ccaagcagct ggagtgatct cacgtgaatt
      781 agaagcgcat ttctttggtc gtgtgggttt atttagaaac caaccaggag atatgctttt
      841 gaaacgaagt gatctagtta ttgctattgg ctatgatcca atcgaatatg aagcaagaaa
      901 ctggaatgcg gaaaaagatg cgcggatcat tgtgatcgat gaagcgccag ctgaaatcga
      961 tccttttatg cagcctgaaa gagaattgat cggtgatatc tcagccactc ttgatttatt
     1021 gacaggttca ttagagcctc aacaagtgtc agaagatgca aaagaatatt tagcatctct
     1081 tcaagcagaa ttaacggagc gggacattgt tcaatcaaaa ggagaagcag gtatcttgca
     1141 tcctttagaa gttattaaca cgcttcagtc aaaagtaaca gatgatatga ctgttaccgt
     1201 agatgtaggt agtcattata tttggatggc acgccacttt agaagttatg aacctcgtca
     1261 tttgttattc agtaatggta tgcaaacgct tggtgttgca ttgccatggg cgatttctgc
     1321 tgcattggtg cgtccaaata cacaaatcgt atcagtttcc ggcgatgggg gatttttatt
     1381 ctctgcccag gatttagaaa cggcggtacg taaaaaattg aatatcgttc atttgatttg
     1441 gaatgatgga cactacaata tggtagagtt ccaagaaaaa atgaaatatc agcgggcgtc
     1501 aggtgtagac tttggtccag ttgattttgt gaaatatgca gaagcttttg gcgctaaagg
     1561 aatccgagct acaagtgtag aggaattaga aaaagcttta gaagaaggat ttgcgacaga
     1621 aggacccgta attattgata ttccgattga ttaccgtgat aatgagaaat tgggagaaac
     1681 aatcttacca gatcaattct attaaggaag tgtcgacatg actgaaaaaa tcttatacca
     1741 gcacgggaca ttaggagctt tgatggctgg cttgatggat ggaacagaaa cgattgctca
     1801 tatattagaa aaaggaactc tgggattggg aacattgcat gggcttgatg gtgaagtcat
     1861 ctttttagat ggcgtagctt atcaaggaag atcagatggc tcagttgccc aattagatgg
     1921 aagtgaactt accccctatg cagcaattac agattttacg ccagatacta gtttttctgt
     1981 ctcaaaaact gcagatggag aacaattgaa aaaagacata ttagtaagag aagccgggga
     2041 aaacctattt ttggcagtga agataactgg attattcaaa aatatgcaca tccgtatcat
     2101 gccgaagcaa gaaaaacctt acagaagact agcaaaaatt tcagagtctc aaccagaatt
     2161 ccaacaaagc aatgtccaag gaacattagt cgggttcttt acgcctgaat tattccaagg
     2221 agttgctgca gcgggatttc acctgcattt tatcgatgat acacatacat ttggcggaca
     2281 tgtaatggat tttgaagtgg ctgaaggaaa agttgagatt agccgcatct cgtcccttgt
     2341 acagcatttt ccagttgatg atccgacttt tgttcacgaa acgattgatt atgctgattt
     2401 agcagcagaa atccaacaag cagaatagaa aaagacagcg agatgcgttc aaggtgatta
     2461 acctgacgta tctcgttttt ttgaggagaa aacctagcgt ttacaaaaaa actgagctat
     2521 aatcttctcc atagatcaaa aaggagcggt ttttatcatg tggacagaaa aacagaaaaa
     2581 agatgcatac gaaattttat tattacagca acaaggatta ttagaagcag aagataactg
     2641 gttagcaaat ttagcaaatt catctgcatt gttgaatgag actttgcctg agactgtctt
     2701 tgcaggatat tatttattcg atggtaatga attgatttta ggaccgttcc aagggcgagt
     2761 ttcttgcaca cgtatcaaga tgggaaaagg agtttgtgga gaatcagctg aaaaacaaga
     2821 aacattgatc gttgatgatg tcaaaaccca tgcaaattac atttcctgcg attcagccgc
     2881 acaatcagaa atcgtagtgc caatggtaaa agaaggtaat ttaatcggtg ttttagatat
     2941 cgattgcgga gtaacgaaag ggtatgatga aatcgatcaa gcatttctag aaaaatatgt
     3001 agatctttta ttagcaaatt ttaatataaa tgcttt
//
LOCUS       DACCXO010000178         2963 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.185, whole genome shotgun sequence.
ACCESSION   DACCXO010000178 DACCXO010000000
VERSION     DACCXO010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.185"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            281..1606
                     /locus_tag="IVY70_002759"
     CDS             281..1606
                     /locus_tag="IVY70_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP7959844.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1599..1949
                     /locus_tag="IVY70_002760"
     CDS             1599..1949
                     /locus_tag="IVY70_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959845.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1931..2116
                     /locus_tag="IVY70_002761"
     CDS             1931..2116
                     /locus_tag="IVY70_002761"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959846.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
BASE COUNT         1047 a          461 c          557 g          898 t
ORIGIN      
        1 cctatttttt gtttttaagg aaagtttttt cttttttgaa tttcttattt ttttctaata
       61 taactaagca aaactggata atacgacact tattttcaaa ttttcacaag actaatagta
      121 aatatccatt taataaagtg atacaatgtg ttgattttac aaatcaacag aagaagtagg
      181 gaaataactt acctatgctt ctttttttgt acatttaatc gattcttctt tacaaaaacg
      241 aacgtatgtt tgtataatgt ggaagaatgg agtgttattt atgatggatt attcaaaaga
      301 gccagtaaat gattactttt tgatcgatat gaaaagcttt tacgcaagtg tcgaatgtat
      361 agaaaggaat ttagatccat taacgactga attagtcgtc atgagtcgag cggataatac
      421 aggatcaggg ttgattttag cttcttcacc agctgcaaag aagaagtacg gtatttctaa
      481 tgtcagtcga ccacgagatc taccgcaacc ttttcctaaa acgttacatg tcgttccacc
      541 acgcatgaat ctctatatta agcgaaatat gcaggtaaac aatattttca gaaggtatgt
      601 agcagacgaa gatttattga tttactcgat cgatgaatcg atcttaaaag tgaccagatc
      661 aatgaatctt tttacgactg aaggaacaag aagtcaacgg agaaagaagc tggctcaaat
      721 gatccaggag aatattaaag aagagctagg attgcttgct acagtaggtg tcggagataa
      781 tcccttatta gctaaacttg ctttagacaa tgaagcaaaa cataatgatg gattccttgc
      841 tgaatggacg tatcaaaatg ttcctgataa agtatggaat attcctgaaa tgacagacct
      901 ttggggtatt ggatctcgaa tgaaaaaaag actcaaccaa attggtatca tgagtattaa
      961 agacttagct aattggaatc catacactat taagagccat ttaggtgtga ttggcttaca
     1021 gctgtatttt catgcgaacg ggatcgatcg aacagatata gcgatcccac cagaaccgac
     1081 aaaggaaaaa tcatatggta attcacaagt gttaccacga gattatacca ggagaaatga
     1141 aatcgaactg gtggtaaaag aaatggcgga acaagtagct attcgtatca ggcaacataa
     1201 ctgtaaaaca ggttgtgtcc atttatacat tggcacgtcg atcttagaaa cgagaccagg
     1261 tttttcacac cagatgaaga tccctataac agataataca aaggaattgc agaattactg
     1321 tcttttcttg tttgataagt attatgaagg acaagaagta aggcacgttg gaataaccta
     1381 tagcaagctt gtgtacactg attcgcttca gctggattta tttagtgatc cacaaaagca
     1441 aattgacgaa gaaaatttag ataaaattat tgacaagatt cggcaaaaat atggttttac
     1501 gtcgattgta catgcttctt ctatgttaga aggcgctcgt agtattacta gaagtacatt
     1561 ggttggtggc catgcaggcg gcgcaggagg gcttaaaaat gattgattat aatgatcgtg
     1621 gacgtttaaa gtgggcaggt ttttatttat ctgatcatac tgaaaaaatt gatgccgaca
     1681 atgaacaacg agccaatcaa aatttggcaa aagaacaaat gacgactgaa gaggttgcgg
     1741 aagtattgaa cgctgcagtc ataaaaaata gatcagtatg tattcaaaaa gaagaacgaa
     1801 acgctgaagg atactatcca gaagatattg ttagcaagat cttaggtagt gatgagctgg
     1861 ggatctacgt tggaaaaaac aaagttcact acgacgaaat acgtcatatc gaattttacc
     1921 aggataataa atggggtcga ttagaatgaa cattatccag caatatgagc taaagtatat
     1981 aacctttgat cagctatcag aagagatttg gggttatggc cagcgtttga taaatgaggt
     2041 tggtgttgaa cgcttctctt tctatgtaga agctgcagca ggataccata aatacgaata
     2101 ctatattggc tattaaagtg caataaaatt atatttcttt tatgttttac attttgtatc
     2161 aattctgata aaattaccta taggtaatta aaaaatataa aaaaactagt tacaacctta
     2221 ggacgttgta actagcttga agcatactag agctaactaa aatagcacta gaaagcaatg
     2281 ttaaacccgt ccaacaaact tgatttaaca ttgagaccag cttattcagc taaatatagt
     2341 tgaataagct tcttttgttt taaaaaaaca aaaggtgtac tacgaataac gcaagtaaaa
     2401 gaaatactat tacaaccagt aggctacaag taagtattac taaacattgc gtagttagaa
     2461 cctttgctct attgtctaaa gccacaaaat tagagatcag ctttatgatg ctaagagcga
     2521 gctgcatcca tgcggacata ggttcttttg acactattaa ttctcctttt taggataagg
     2581 gaaattgcta gtagtttttt cattagcacc atcccccttc atttgattga gtctagtgct
     2641 gctgtgatag tttgctgttt aatatgctta cattcttatt ataacataaa tgaaacaaac
     2701 acatggttag aggtcgtatc aatcaaaata gtgtctcaca aacgatttat tacttactcc
     2761 tgctgactaa cggactgttc ttcagtaact gtaactatat gtttgttaaa accaccatta
     2821 cctgtagata aaaaccaata tatttactat actcactata ttaaatatat ttagtatagt
     2881 aaaaactaga aacaataaaa tttgtgaatg ttgtataccc ccaaacaata cacatatttt
     2941 gaaggattag gattaataaa gct
//
LOCUS       DACCXO010000179         2915 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.186, whole genome shotgun sequence.
ACCESSION   DACCXO010000179 DACCXO010000000
VERSION     DACCXO010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.186"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(32..979)
                     /locus_tag="IVY70_002762"
     CDS             complement(32..979)
                     /locus_tag="IVY70_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP7959847.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(1157..1960)
                     /locus_tag="IVY70_002763"
     CDS             complement(1157..1960)
                     /locus_tag="IVY70_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP7959848.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            2106..>2915
                     /locus_tag="IVY70_002764"
     CDS             2106..>2915
                     /locus_tag="IVY70_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP7959849.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIIS"
BASE COUNT          940 a          470 c          565 g          940 t
ORIGIN      
        1 ttttttcttt tgaaaatatt gttttcagct attaatatcc tgcagacaat tttacttgat
       61 tggtttgaag ctcatgggtg gcaaagtagc ttttcagatt ggttaaaaaa atagccatta
      121 atttcttttg aaaatcaggt gtcaaacctg caatgtggga agtaatcagt acattgtcca
      181 ttttccaaag aggatgatct tgtggaagag gctcttcctc aaaaacgtct aatgctgcaa
      241 aggaaagttg ctggttattt aaagcctgca ccagatcttg tgtcttaaca gaagcaccac
      301 ggccaacatt gatgaataca gattttggat caaagtgttc aaacatatgc tgatcaaaga
      361 gcccagttgt ttcctccgtt agaggtaaga tattgactac gaaatctact tcaggaagaa
      421 cttcctttag ttgatttaaa gaaaaagtct tttccattcc ttctgctgga tgaccggatg
      481 tattgattcc atagcagttc atgtctaatg ctttgcattt ttttgcaatc atttttccaa
      541 tatggccagt tccaatgatt agtatttttt tcccttggat agattggtaa tggaccgatg
      601 aacctaacca tttcatgtca ctctgtgcct tttgtgcttg aaaaagacca cgagaatacc
      661 caaggatgat tcctaagaca tgctccgtaa tcgattcact gtggatgcca ctaccatttg
      721 aaagtaaaat atgtttcttt gaaaaagtct caaggggtag ctgatcaaca ccagctgaga
      781 tagattggac ccatttcaag gtagaagagg ctaatagttt ttcttggtag tctgagttcc
      841 agcccaaact gatctctaca ttttgtagat cctctggttg taattctgtt ttaaagatat
      901 aaccaggcgc tatagtatgg atttcttcga taaattcatc acgaaatgga cgatctgcat
      961 aaatgattct ttctgacata gttaatttcc cgcctttctt tactctaagt gtagcgcata
     1021 caagaagaac tttgaagcgc cttgcttgtt ttttgagaaa gaaatatttt tcaaataaaa
     1081 aaggggctgt gacaaaaaaa tgtcacaacc tctaaaaggg tctattgaag tagaaataac
     1141 gagttgtctt tatttcttaa ttgatcgata tttttgccca tcctttgaca gcttgagcaa
     1201 aaataaagat tcctgcaaaa gcaatgaagg cgctagtgtc aaaagaagga agaaagacca
     1261 tcaagattgt tgcaattata gtaggcaatg aaattgaata gatcaatgtt tttaaacaat
     1321 caagaaaagt agcttttgtt agtcgaaagc gtgcatagag aaaagcagca atgttggtaa
     1381 atagcaaagt gatcagcaga ttcagaaaac taggataaat agaaaatata aatgtcagga
     1441 cttttatcca aagtggtata gatgcttcac ttaaaaaaga acggagctgc ttacctgtta
     1501 agttttttaa agattcattg gtgtagggaa gttctaattg gttattgttc aacaaagcag
     1561 aagtcgtgcc tgtattagga agagctatga taagtttgtt ttttaacatc ccgacactaa
     1621 caaaattccc catcaaatca gtagagatgt cctgttctgt tcgttttccc tctggatcaa
     1681 atgtgaagat aatggagttt gtttggtaaa tgaatccttc tttttcttcg gtttgaagct
     1741 gcccgttttg gattgtaaaa tcaggaatgc gagtagcaat cttttgccca tccaattgaa
     1801 tgttgtttaa gacttgaaaa acttgaaaag agattggcaa agccataatt atactcaaaa
     1861 ctaaaagata gacaatggct ttccaaaatg ggacatttct cgcctccttt aggtctttga
     1921 atcgtataaa ggaactgata attaattgtt ttgttgtcat aagtactcct catctagtag
     1981 tgtcatcact tgcaattcta gcgaagtatc aagtgaaata caagtgcttt aaggaaacag
     2041 ctgaatgatt ggtgaaatat caaaaaaatg ttattcttgt ctaaagacta tccaatataa
     2101 ataaaatgga ggaaggaata gtgcctacac agccacagag atcatcaaaa aaactatttg
     2161 gagtagtggt tttgaatgcg gtgatcactt tatcagaatt tattggaggg attatgtcag
     2221 ggagtttgtc tcttgtttca gatgctttcc ataaccttag tgatacattg gcaattattt
     2281 tttcttacgg ggcacaaaaa atggcccgaa aagaagcaaa taaaaaacgt acgtatggct
     2341 accagcgttt agaaatattg agtgctttca tcaactcatt catattgatc atattatcct
     2401 tgtttttaac cgttgaagca ttcaagcggt tcacttcacc agaaaagata aatagtcatt
     2461 tgatgctaac agtagcagtt attggattgc tcgcgaattt gttctcagta ttattattac
     2521 gacaagaagc agatgaaagt ttgaatataa aatctagtta tttgcatttg ttaagtgata
     2581 cactgtcttc ggtaagtgtg attatcggag caattttgat ccgttttttc gatatctact
     2641 ggatcgatcc agttattaca ctgatcattt cgatatatat attgatagaa gcgataatgg
     2701 tgatcaaaaa agctgccgct attttgatac aaagtgcacc tgctatcgac tatgaaaaaa
     2761 tggaacaaga aattgaagca atcgatgggg taaaggatgt tcatcatgtc catatttggc
     2821 agtatagcga aaagataatt attttcgacg gacatattga ttttgaagat cagctactaa
     2881 gcgagattga aaagatatat cctcgtataa tttct
//
LOCUS       DACCXO010000180         2839 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.187, whole genome shotgun sequence.
ACCESSION   DACCXO010000180 DACCXO010000000
VERSION     DACCXO010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.187"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(641..1843)
                     /locus_tag="IVY70_002765"
     CDS             complement(641..1843)
                     /locus_tag="IVY70_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HAP7959850.1"
                     /translation="MCTKLLQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNT
                     LYLDKSLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAK
                     GLFVEKLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYE
                     SAMIQGFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDY
                     QLPEGSRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEM
                     ILNDVNNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTE
                     VNINEYSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVT
                     EYIKNYFN"
     gene            1864..2637
                     /locus_tag="IVY70_002766"
                     /pseudo
     CDS             1864..2637
                     /locus_tag="IVY70_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2705..>2839)
                     /locus_tag="IVY70_002767"
     CDS             complement(2705..>2839)
                     /locus_tag="IVY70_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7959851.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          887 a          497 c          470 g          985 t
ORIGIN      
        1 cgtcatgtga cataattgta ttgtatccgc tttctttgtg tttgtcaata ggaaaaattt
       61 atttctctaa aaaatataaa taatctgcag tgaaacttga gtacatatac taatttcgaa
      121 aaatgcctat gatttaaata attatggtgc tgggatgaag gtttataagt ttgtcttgat
      181 ttgtttagaa gaaatcgttt tgcacacagt tgaattagga aactactgga gtggaaaata
      241 aagtgatttg acgggtagat gggtgcactt aaatttgaaa atggcttaaa atgctttatt
      301 ttgaattgca gaatttcttt tagtacgcaa tttatggtct taaaattgat taaaaagtga
      361 tacattgatt ttaatacaat ttatgataca aaaaatgacc tcctttctta aataaaagag
      421 gtcattttgt tttgtctcat aatcgattgt ttttgataca gtagttatat tcagaaaaaa
      481 taaagactct tctgagttta taatcctttg gattatatac ggatacagat gtagacataa
      541 aacaagctca attttcaaaa atggcattac tcaccttttt ggtgaattat taaactaaaa
      601 aagatgaatt tttttatgta ctataagatg taggtgtact ctagttaaag taattcttta
      661 tgtactctgt aacatcgaat acatcttcta ataaaaatgc acgcaataat ctgtcaatta
      721 gcatatttac cttgattggt attaccattt tacctaaatt tttctcgata tatccactga
      781 aatgacctac gttaacactt gattttaatt tatcaaatcg actatattca ttaatgttta
      841 cctcggttaa gcgtaaatcc aacatctcac gtaataatgc ctcattaata ttggttgaat
      901 ccactaaatc ttggataaaa tcgtcctctt cactaagctg atattcattg aggtaatcag
      961 taaattccca tccttctttg ataactaatt ccccattatt tacatcattt agaatcatct
     1021 cagcatatgg ctgtaactcc actggtaatt gcgcaaatgt gcgatgtaag tcgtttaaca
     1081 atgattgaat taaattcgaa tcacgttctt cttttatatt catgtatttt ttgaagtttg
     1141 tgtttaaata ctcataatta atacgactac gactaccttc aggtaattgg taatcaatgt
     1201 catacgctat actatcattt ccgtctctag gttctctctc tatctcgctg taacgttgtg
     1261 ccaagatatt aaattcatat tctgtgatta ctactgttat aggttcataa tcctggattt
     1321 cataagtcga ttttgcccat gtgaatccct gtatcatcgc tgattcataa caatggatta
     1381 gttgattata aagatttaca aattgcttaa tttcccctgg aacctccggt aaatgactga
     1441 agttttctat gccagaaata ttaaatacgg acttaatttc ttcgaatata tcgttcaact
     1501 tttctaagtt attatataat ttttcaacaa ataacccttt tgcttgtttg cctgagtata
     1561 actcaatagc ctcttcaatg ttctgttcca tcgtatgtgc tttacgataa taacgaatgg
     1621 ttccgaacgg tttttcagga ccattaagac gatttgtgcg tgaaaaagcc tgaattatgt
     1681 tctgatattc aagtgactta tctaagtata gggtgttgac ccattttgaa tcgtaacccg
     1741 ttaagagctg attaacaact ataacaagat ctaattgggt tgcttcatct actgttttgt
     1801 aaggattctt atgcgctgtg ttttgcaata gttttgtaca caagattgca aagaaaagta
     1861 cacaatgttg ccacttctgg ttccatgagt tttatttgtg ttgatgggac aaccaatcag
     1921 ctatttacga cccttgaaga tcgcagactt tacaaactaa ctcaatactt cctgcgcttt
     1981 cctagaaagg cccgtttaaa ggtcaagtac ctagtgatgg acatgaatgc cagctattgt
     2041 cagttgctta aaacggtctt tccaaatgcg gagatcgtaa ctgaccgctt tcatattgtc
     2101 caacacatca atcgcagctt caatcaatta cgtgtccaga tcatgaatcg ttttcgaacc
     2161 tcacactctg aaaatcagaa gaagtaccgt cggctcaagc gatattggaa actcttactc
     2221 aaagattcca cgacatggga ggcactcaaa cggcattatc atcgactgtt taaacgacca
     2281 attagtcaaa cagaaatcgt taatgagctg ttaagctaca acgaagaatt gcgaactgcg
     2341 tatcattttt gtcaattatt acgctattat ttcgttaaac gagatacggt aggctttcaa
     2401 gaaaccctta aaacaatttc atctacgtta cctcagtcgt ttaaaaagaa attcacgatc
     2461 ttccatcgct accaatcggg tatttcaaat gcctttaagg tgtcccactc aaacggcgtc
     2521 actgaggggc tgtataacaa gatcaagctg attaaacgaa ttgcgtttgg ttatcgcaac
     2581 ttttataact ttcgggcacg gatctattta caacaaggtc tgatctttga aaactaaaaa
     2641 aatatgcggc gcacagaacg cgccgcatat cggactaagt tttattggct tattatttgt
     2701 caaattaagc gagacaaaac tttccattta cgtaactcaa aaaaacttct aatggtgttt
     2761 ggtaatttag tgattttcta gggatatggt ttcttttgga cgcaacggat gagatgaatc
     2821 cttgattgac ttggttgaa
//
LOCUS       DACCXO010000181         2838 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.188, whole genome shotgun sequence.
ACCESSION   DACCXO010000181 DACCXO010000000
VERSION     DACCXO010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.188"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..1145)
                     /locus_tag="IVY70_002768"
     CDS             complement(<1..1145)
                     /locus_tag="IVY70_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAP7959852.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGIT"
     gene            complement(1313..1513)
                     /locus_tag="IVY70_002769"
     CDS             complement(1313..1513)
                     /locus_tag="IVY70_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7959853.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(1647..2255)
                     /locus_tag="IVY70_002770"
     CDS             complement(1647..2255)
                     /locus_tag="IVY70_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP7959854.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(2340..2810)
                     /locus_tag="IVY70_002771"
     CDS             complement(2340..2810)
                     /locus_tag="IVY70_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP7959855.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
BASE COUNT          865 a          569 c          477 g          927 t
ORIGIN      
        1 gtgatgcctt cttcataggt cagaaggttt tggagctttt cccgaaagcc gtcatctaac
       61 gtttcgggaa gttctgccaa taaggagaaa aataagtcag ggtctttgtc tcgaaaggct
      121 tcaactaatt catgaaaata gggatacgcc tccttcaggg acgtagaaaa gccaagcaat
      181 cgatcaatca tcatcgcttc cgtaagaaat gggtattttg gtgctcgaaa acttttccat
      241 gttttgtatt cataatggtt gatgtttgca cgatttttta gcaaaaagcg ccagttcttt
      301 ttcagttttt ctgcctgact tttctgtcct actttacgaa gttcattcat ttcacggata
      361 cgcaactcat taaaggcttg attcatgtgt ttgataatat gaaaccgatc aatcactact
      421 ttcgcatttg gcagaatacg tttggtaagc tggaagtagg cggcgttcat gtctgtgact
      481 aaaaatttta cttcttcagg attagcgcag cctaagaaat agcttgttaa tcgaggcaat
      541 ttacgcgtag gcaagacatc aattaattgt cctgtttcgc catctgcgca aataaagctc
      601 attttatctt ctatggaagc gtgcgaacga aattcatcaa ccatcaatac tctggggaga
      661 atcctcttag attgctttgg taagtagctt ttaaactctt tcaacgtacg aataacggtg
      721 gtcaaagata cctgacaatt tttcgcaata aaagataaag atactttttc agtcagtaaa
      781 gaagcaattt tatatctgac atgattcgcg attgaatgtc tgggttgaac aaaataactt
      841 tgagccgtcc aatgggttcg acagttttta caggtatagc gctgcttttt taagcgcata
      901 accaaaggca tgtgattgta ttgttcaaag cggacaatcg tttccttctt cccatttttc
      961 actacaatta ctttcccgtt tccatctacc acagtagaac cacaatttct acaggtatgg
     1021 ggagcaggtg agaaaacagc atcgacgatc aacgtctttt tcttctgaaa ggtctcgtaa
     1081 gagacctctg taatcatcaa atctttctct attaatcgca gcattttttt gatagaatca
     1141 ttcatatagc atatcgtcct ctcagttgtt tattttgtcg tgatttaatc atactagaga
     1201 acgatatgtt ttttaatacc taaaataaaa atggggctga agaatcaatt ctgattcatc
     1261 agtcccataa attatagagc cctaagaagt cgtgtcttta ttcaacaaat tattataatt
     1321 tagtcacttc agcagcttga ggtccgcgtg caccttcaac aatcgtgaat tctacttctt
     1381 gaccttcttc taggctcttg aagccgtctc cttgaattgc tgagaaatga acaaatacgt
     1441 catctccgcc gtctactgta ataaacccaa atcctttttc attgttgaac catttaaccg
     1501 ttccgtgttc cataaatgta tatcctccaa aaaagttctt gcaagtaacc ttgcaagaac
     1561 attataccca atttagaaaa cgctagcaag ctttcgctct tttattttcc aaattatttt
     1621 taagactgat atgacggagg aaatggttat tttgctgctt cgaaacgttt gtttacttca
     1681 tcccaattaa taacattcca gaatgctgca atataatctg gtcggacatt tttatatttc
     1741 aaataataag catgttccca aacgtccaaa cctagaattg gtgtctttcc ttccatcaat
     1801 ggagaatctt gatttgcagt agaggtaata gctaattttc cattttccat tacaagccaa
     1861 gcccatccag aaccaaatcg tccagcggct gcttttttga attcttcttt aaaagaagaa
     1921 aaatcaccaa aagcttcatt aatcgcttct tttatttctc ctgtaggttc gccaccagca
     1981 tttggtgcca tgatttccca gaaaaatgaa tggttagcat gtccgccacc attgttacgt
     2041 acagctgtct tgatatctgt tggaatagcg tccatatcag ataataattc ttctattgtt
     2101 ttttcgccta attctgggta tttctcaatt gctgaattta aattcgtcac ataagtattg
     2161 tgatgcttat catgatgcag atgcatcgtt tcttcgtcaa tataaggttc caatgcgtca
     2221 tatgcataag gtagatctgg taatgtgtaa gtcataaaaa aattcctcct caatgttttc
     2281 ttaataatgt ctacattaca aagagtaaca aagagaagga aattcttcaa ttgttatgcc
     2341 tagtcgtctt tttcactttg acctacttct tcttcaaaaa cctcagtttc tttaaaaata
     2401 aacaacttgc tgaaaatata gttcgcaagg ataacaataa tttgagtgat gattttcgcc
     2461 acatactcat tgctgttcat aacattgatc aacaatacca ttgcccccat gtccaatccg
     2521 taagaaagga tacggtagaa gaagaactta ccgcattcaa taagtgcatg tgtaaaattc
     2581 ggtgattttg aatggaatac ccaaagtttg ttcgttacaa aagcaaacaa tacagaacaa
     2641 aaccacgaaa tagaattgga taaggccata cttaaatcaa aatattggcg tgcgagagca
     2701 aaactaacaa aatttacaat cgtagccaaa ccaccaaaaa agagatagac tagtatctcc
     2761 caatatcctt ttgcttctaa atatcctttt aactgtcgat actgcttcat gagccaacct
     2821 accttattaa aaattctt
//
LOCUS       DACCXO010000182         2820 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.189, whole genome shotgun sequence.
ACCESSION   DACCXO010000182 DACCXO010000000
VERSION     DACCXO010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.189"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            14..1141
                     /locus_tag="IVY70_002772"
     CDS             14..1141
                     /locus_tag="IVY70_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7959856.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1359..2501)
                     /locus_tag="IVY70_002773"
     CDS             complement(1359..2501)
                     /locus_tag="IVY70_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7959857.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2506..2691)
                     /locus_tag="IVY70_002774"
     CDS             complement(2506..2691)
                     /locus_tag="IVY70_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP7959858.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
BASE COUNT          933 a          517 c          447 g          923 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa ccaccggtgg caaaccggtg gcaaatccat gtaaacatgg
     1201 tttatttttt acactgtttt caaacaaacc cttgctattt cttcggttcg tcctcgtcca
     1261 ttttcgatac cgacagctag cttataaccc aaggtatatc aacataaaaa aataaaaata
     1321 ggctatgaca gcagcgtgac agcaaaaaag atggaaagtt aaatatcaaa cctcgtaatt
     1381 tcatttgcga gtgcttgatt tgtttctgga tataagtgtc catatttgtc caaagtagtt
     1441 ttaatagagg catgacctag acgcttagat attgcataga tatttttatt atgttcaatt
     1501 aaaaaggcaa catgcgagtg tcttaatcca tgcatatgaa ttggttttac tcctgcaatt
     1561 tcagctagtt tttttattct gcttaaaata gttcttggag atggcggtaa tccatcaaaa
     1621 gaaaaaacca attctatatt tttcccaatt gattgttgag tttttttcca tgtttttaaa
     1681 tcttcaacgg tttgtctgtc taacgaaatt atccgaatac ttgatgtatt ttttgtttca
     1741 gcaaattcat aatcttgtct tgtacggatg taaattgact tatttacgct acatctgcca
     1801 tttattaagt caaagtcttt ccattgtaac gctaagagct cctcactcct tacacctgta
     1861 acaaaaataa aacgcatcaa acgtttataa aattcttcat aaaaatcccc gtgataaaca
     1921 ctattatata ctttcttaaa ttcatcaact gtccaaaatt ctacttgagg acgctgagtt
     1981 tttattttac ctatagtatc tacaggattt tcttcaataa tttcaagtac aattgccctt
     2041 ttaaaaacta ctctcaactt attgaatatc tgtttaatat aatttggtga taatggttgc
     2101 tcaatgccct tttttacaac atggattttc gataaaaact gttgaaattc ctgtatatga
     2161 attggtcgaa tctttacaac ctgcatttct ccaaaaaaat ccaatgcacg ttgtaaaata
     2221 tgatctgttt tcatatatgt tttttctaca gttcccaact tataccaagg aaaaaaatac
     2281 tctcgataaa aatcatgaaa cgttattcct tgcagataaa ttccttgtga atcagaaaaa
     2341 ctttgtttta actcttcgag tccccttcta gcttctcctt gagttttgaa gcctcttctg
     2401 gtcacttgaa ttctttttcc aaagcgatct tgtcctaaag ttgcagaaaa aaaccaagtg
     2461 cctctttttc tgtctttata aacattcgca attttcctca ttttactatt cctcccaaga
     2521 aatatggaga acttcttcta aaattgaaat aggaattctt tttatttttg aattacgata
     2581 aaaaaagtat cctttctcaa ctaaaagttc tttacattcc ttgaagattt tattagcata
     2641 gtactgagaa aaacctagat taatcaaatc ttttcgagta gctgttttca ttatagcacc
     2701 ttccttactt tttttcattt atttctatca gctgcttttg tcaaaagcac ataggaattt
     2761 cacctctccc caagtttacg gcgagcctta cggaagtatc attatcccaa ctttaaatca
//
LOCUS       DACCXO010000183         2726 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.191, whole genome shotgun sequence.
ACCESSION   DACCXO010000183 DACCXO010000000
VERSION     DACCXO010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.191"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..1097)
                     /locus_tag="IVY70_002775"
     CDS             complement(<1..1097)
                     /locus_tag="IVY70_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7959859.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..2603)
                     /locus_tag="IVY70_002776"
     CDS             complement(1227..2603)
                     /locus_tag="IVY70_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7959860.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2603..>2726)
                     /locus_tag="IVY70_002777"
     CDS             complement(2603..>2726)
                     /locus_tag="IVY70_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7959861.1"
                     /translation="RNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE"
BASE COUNT          834 a          521 c          386 g          985 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acgtactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
     2401 ggtgttcctg gatttacttg tctatttatc tccatagaaa aactatttgt tatattagca
     2461 aatgtttctt ttgaagctct gtccgtagca tctttaatag ataaactaat gagacttaaa
     2521 gttgacatag ataaaataac taatatgata attaatgatt ttaaactttt tctagttaca
     2581 taagcaaatg cattttttat cattattcaa cctccaaatt cattttattt acttttttca
     2641 actttttacc acttagttct aaaatgatat ccgcagaatc tgctacttcc ttactatgtg
     2701 ttacaactat tacacattta tttcta
//
LOCUS       DACCXO010000184         2687 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.192, whole genome shotgun sequence.
ACCESSION   DACCXO010000184 DACCXO010000000
VERSION     DACCXO010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.192"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..263
                     /locus_tag="IVY70_002778"
     CDS             <1..263
                     /locus_tag="IVY70_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959862.1"
                     /translation="AQLWNMMYFNIGPKVHEELDFCFATDPIEKVKEVKILHNAGVTT
                     NDEDLFFKGRYVTSTPFDEDLSFVNKKKCSYAYVKAIKAVVR"
     gene            260..571
                     /locus_tag="IVY70_002779"
     CDS             260..571
                     /locus_tag="IVY70_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959863.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            561..914
                     /locus_tag="IVY70_002780"
     CDS             561..914
                     /locus_tag="IVY70_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP7959864.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            904..1305
                     /locus_tag="IVY70_002781"
     CDS             904..1305
                     /locus_tag="IVY70_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959865.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            1298..1702
                     /locus_tag="IVY70_002782"
     CDS             1298..1702
                     /locus_tag="IVY70_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959866.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            1714..2322
                     /locus_tag="IVY70_002783"
     CDS             1714..2322
                     /locus_tag="IVY70_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7959867.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            2342..>2687
                     /locus_tag="IVY70_002784"
     CDS             2342..>2687
                     /locus_tag="IVY70_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959868.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKE"
BASE COUNT          993 a          393 c          537 g          764 t
ORIGIN      
        1 gggcacagct ttggaacatg atgtatttca atattggtcc taaagttcac gaggaattag
       61 acttttgttt tgctactgat ccaatagaaa aagttaaaga agtaaaaatc ttacacaatg
      121 ctggagtaac aacaaacgat gaagatttat ttttcaaagg gagatacgtg acttctacgc
      181 cttttgatga agatttatca tttgtaaaca agaaaaaatg ctcttacgca tatgttaaag
      241 caattaaggc ggtggttaga tgacgcctga acaagtgact gaagaattgc taatagctgt
      301 gaaggataat atttacgtta cctggaacga agaagatgag tcaattaaaa agatgatagc
      361 taaaaatgct gtttatcttc aaagtaaagt gagtacaaca ctttcttttt ctcctgaaag
      421 cttagaatac ggattgctaa tcgaaagatg tagatacgac tggaatcgtg ctttagatga
      481 gtttgaacaa aatttcgcta gtgagttatt aggtttcatt caacattatg cgctacaaga
      541 atatattgca ggtgatgtga atggcgaata atcgtagact cgaagaaaca ttcaacgatg
      601 gttggttaaa gattttgacg caaactacca aaagaaatga actaggaaaa aagattggtg
      661 tagaagatac agaaatcact tctttaaaat ttagaaatct ttccatgaga gatagtgata
      721 taacagctat ggatgcgatg ggatcgaaat taactaagaa agtaaagact ccatttcatc
      781 caatcgccaa gaaatttaat aaagatcaat attttatcgt aatcgatagt atgcgttaca
      841 acgttatcta tgccgattac gataattttt atatctattt ttatcttgaa agtgtgggtg
      901 aatatggtga ttgataattc taaagaaaaa gaacgtttaa ataagcaaat ttctgctatc
      961 aaaacttcct tagaagagca ttttggcctc aaactctttc aagactccgt aggcgaggat
     1021 gagctacctg atgattttaa ttacttcatt ctcgaaacag gagaaataga aatgatcact
     1081 gagccaaaat atagcgtggg tcaaaatcta tatctaactt tctattcaga aaatagagaa
     1141 gatttaacag gagactcact agatattatt tcattgattc aaaatcgttc gattcgtttt
     1201 cagagaatgg atcccaatca tttaaaacta gagaaccaag atcgctatat cgatcaattg
     1261 gtatttacgt ttagacgatt attgaagagt gattgtcatg gctaaaaata gttgggagct
     1321 aaaaataaat ggacatgatg aacttcttgt gcggatggaa cgctattcaa gcgagagcga
     1381 acgactgatt aacgaagcat tgaaatcaaa aggttcggct attgcagtgg ataggattac
     1441 agaaaaaatt cctgtttctg aagcagattt aagaagaggg caccaacacg caaaaaatag
     1501 tcgtccactt aagactcaat acattaattt gggtttcatc attagaccta caagaaaatt
     1561 tgagtattta aaatatcctg atttggggat aggtacttct aaaagaaatc agccggacga
     1621 atttatgaga agaggattag gtcttgcact tgatccaatt acagaacttc tgattcgtca
     1681 attcgataaa ttaaataaat agggggaaca acaatggcta aaacaaaaac tgtagtaaca
     1741 acgttcgata acgtaagtat caaacgaatt gcttttaatt ttaagaacgc aggaaatgca
     1801 atcgcaacga attgtaatgg acaattagat ggcgaaacag aaatgcaaac ggtggttaaa
     1861 aaatgtggag cgacagaagt aaaatcaaaa tctaaaccaa tcaatatgac ggtaacaatt
     1921 actgcacatg taccgatgga agtttatcga cgtttcaatg ggttgaaaca agatggacgt
     1981 attaaaccag gcatttactc ttacggtcct gattccgtag gcgaagattt ctcacttgct
     2041 gcagagatcg tggatgactt cgaagaaaat agaaagttag ttggtatgtt agcatgcact
     2101 tcgaatacag gattaacatt ctctattgaa aatggtgcgg atgaagtagc tgcgttagaa
     2161 ctagaaacaa aagttatgca agatgaattt ggtaaatttt atcatgaagc aattgttgca
     2221 gaacttgaag aagacttaac agatcaatgg atgacgaatc tatctgctga tgtgattaaa
     2281 aaaacttcag ttgtgacaac tacggccact caatcacagt aaaaaaaacg gaggtagcga
     2341 aatgaacgaa gattactcaa aaattgaact aaacgatgga acaattttga atttagaacc
     2401 taaactgaat atcaagaaat tattgatgat caatagagat tttaacacag acgagtttgc
     2461 aaaaatgact gtgggaaaag gatccatgga tatttctgtt attcaaggtg caaaggctgt
     2521 gtatattgct taccgccaag cgaacatgac tgattatatt tcattcgatg aatttatcga
     2581 taaatgggat tttgatatgg ctactgccag ctatatttat caattgatga tgttcaaaca
     2641 agcacgcgat gcttatcaaa aagaatttga aaaagcaaat aaggaaa
//
LOCUS       DACCXO010000185         2682 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.193, whole genome shotgun sequence.
ACCESSION   DACCXO010000185 DACCXO010000000
VERSION     DACCXO010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.193"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..311
                     /locus_tag="IVY70_002785"
     CDS             <1..311
                     /locus_tag="IVY70_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP7959869.1"
                     /translation="PHQLFMADISTFEEGKNAYKAGIDFVGTTLSGYTEESKKQDGPD
                     IDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQEIASRFVKALS
                     "
     gene            325..1827
                     /locus_tag="IVY70_002786"
     CDS             325..1827
                     /locus_tag="IVY70_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7959870.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            1840..2316
                     /locus_tag="IVY70_002787"
     CDS             1840..2316
                     /locus_tag="IVY70_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP7959871.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(2413..2523)
                     /locus_tag="IVY70_002788"
                     /pseudo
     CDS             complement(2413..2523)
                     /locus_tag="IVY70_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          878 a          361 c          527 g          916 t
ORIGIN      
        1 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
       61 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
      121 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
      181 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
      241 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
      301 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
      361 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
      421 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
      481 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
      541 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
      601 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
      661 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
      721 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
      781 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
      841 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
      901 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
      961 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     1021 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     1081 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     1141 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     1201 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     1261 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     1321 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     1381 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     1441 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     1501 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     1561 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     1621 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     1681 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     1741 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     1801 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     1861 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     1921 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     1981 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     2041 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     2101 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     2161 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     2221 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     2281 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     2341 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     2401 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     2461 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     2521 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     2581 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     2641 accttagcac tatataccaa atatagtaaa atgctgaaag at
//
LOCUS       DACCXO010000186         2606 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.194, whole genome shotgun sequence.
ACCESSION   DACCXO010000186 DACCXO010000000
VERSION     DACCXO010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.194"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..563)
                     /locus_tag="IVY70_002789"
     CDS             complement(<1..563)
                     /locus_tag="IVY70_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7959872.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGVNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLA"
     gene            complement(574..771)
                     /locus_tag="IVY70_002790"
     CDS             complement(574..771)
                     /locus_tag="IVY70_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP7959873.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            complement(787..1029)
                     /locus_tag="IVY70_002791"
     CDS             complement(787..1029)
                     /locus_tag="IVY70_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP7959874.1"
                     /translation="MKDEAIQDVVERLVRIETKLDNYESLREKAESAKDRADQAYSIA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            complement(1086..>2606)
                     /locus_tag="IVY70_002792"
     CDS             complement(1086..>2606)
                     /locus_tag="IVY70_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959875.1"
                     /translation="FGEYDYSGNPNLSAKLNASSFSLGTGATVADDNDEIVFTLDGTN
                     QLSKYASKAQVPVAEGKQYTISCEIMLEDGFTGDPSGIRLQHAYLPGGVVVLQTETVP
                     KNELNTWQTLIGTRTVKYTSDVPNEWYPLFRDIRTLKPTGKVRLRKIKIEEGSTATPY
                     QPNLLDDPYWLGKAPLGENIANKSVTFPIKSSAYSLYQANMEEEFVLGQTYTITMKAT
                     KPPIQTFIVYNEDSGDYRYGNLEPVEGLVDTWGLTFTPQKVGVNYPKRLTIIQYPQST
                     VGACRIDWLKIEKGDTRTPNISEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDVK
                     ISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANETAIKSTNSRVNEI
                     ENIITALTKEETYLLPASIVSGGSLKFTSRGGMTVATGSFKLAKDIAFGEVLVNDLPD
                     SMINIDNNAIGTGAVTGNSVSQIYVESNTRKVVANTAMKKGQWFTVGFSYHSKLSI"
BASE COUNT          705 a          580 c          439 g          882 t
ORIGIN      
        1 gctaattttt ctttacttac gcccatttca gaaagataac cgtaaggatc tgtatgatcg
       61 ccccagatgt tttgtgttac ccataaatgc gatttgattc ccggttggtt ataaggagtg
      121 tccaacgtta atggaatacc atatttcatt gctgaatctc tagccaattc aacgtatgcc
      181 ttgtagtttt tctcaaacgt tgctttatca tgtgtgtgtt gtaactcaat ctgcacagga
      241 ctgttggcat tagcatacga accagcaccg tactgtacat aaccaggttg accgacttga
      301 taaacaattc cgccgtctcc cacaatataa gcagtataag cgctagtcca tgaacgttgc
      361 atatactgcg cttcattgcg tcctgttgct gtttcattag ccgtttcatg cagtaaaata
      421 tactgattat ttgctacttg tgagctacct tcatttacac ctaaattaaa ttcattgttg
      481 atcgtatagg caaacccatt aattggcaat aaaaaaagag ccgttaacag gctcatcgca
      541 gtaatagtaa ttttcttttt catttgtttc ctcctatttt ttcaaattat aagccgacac
      601 accagtgata acgcctaaaa atgttgctac tgcattgata gtgagtactg tcatatctgt
      661 tccattccat ccatacgctt ttcctaacgt ggctactaac acagaagcag ctggcaatac
      721 tgttaaaact gtccatttaa tgacttgata atacttatct ggtaaaatca tttcttctca
      781 cctcctttac aatttagtca agaaatagcc aatgatcgta atgcctaaac cgatcatgta
      841 accccacgac catttattat tggctttcat ttctttgata tcttccgcat tattaagcgc
      901 aatagaatat gcctgatccg ctctatcttt tgcactttcc gctttttcgc gtaatgattc
      961 gtaattatcc aacttcgttt caatacgcac taaacgttct accacgtctt gtattgcttc
     1021 atctttcaac caactagcct cctttcattg caaaataaaa acgtactcga atgagtacgc
     1081 taaatctata tagataattt actgtgatac gaaaatccaa ctgtaaacca ttgacctttt
     1141 ttcatagcgg tattcgcaac cacctttctc gtattagatt caacatatat ttgcgataca
     1201 gaattacctg tgacagctcc agtccctata gcattgttat caatattaat cattgaatct
     1261 ggcaaatcat taactaaaac ttctccgaat gctatgtcct tagccaactt aaatgatcct
     1321 gtagctactg tcattccacc acgacttgtg aatttaagag atccgccact tactatacta
     1381 gcaggtagca aatatgtttc ttcttttgtt agggccgtaa taatgttttc tatctcatta
     1441 accctactat tcgtactctt tatagcagtc tcattagcgt tcgcttttgt ttgagcatcc
     1501 ttagctgtgg tatctacttc attaatagaa gcagtcaact gcgaattaat ctccgatact
     1561 ttcccatcgg tataattgtt tgctttacca gtaatttcag aaattttaac atctgtagca
     1621 agattgtctt cgacatattc tggtgctaga tcccaaacat aatcttttgg attgtttgaa
     1681 tcacgcatac cagtaccacg atatttatac tcactaatat tcggggttcg tgtgtcgcct
     1741 ttttcaatct tgagccagtc gatccggcat gcgcctactg ttgattgtgg atactggata
     1801 atagttaacc gtttggggta gttaactcca accttctgcg gtgtaaaagt cagaccccat
     1861 gtatcaacta acccttctac tggctctaag ttaccatatc tataatctcc actatcctcg
     1921 ttgtaaacaa tgaacgtttg gataggtggt ttggttgcct tcatggtaat tgtataggtt
     1981 tgacctaaaa caaattcttc ttccatgtta gcttggtata ggctgtaggc gctagatttg
     2041 attggaaacg taacagactt attagcaata ttctcaccca aaggcgcttt acctagccag
     2101 taagggtcat caagtaagtt tggctgatat ggggtggctg ttgagccttc ttcaatctta
     2161 atttttctta gtctaacttt accggtcggt ttaagtgttc ggatgtctct aaacaaaggg
     2221 taccattcat ttggtacgtc agaggtgtat tttaccgttc gcgttccgat aagcgtctgc
     2281 cacgtattca attcattttt aggtactgtc tctgtctgta aaacaacaac cccgcctggc
     2341 aaatatgcgt gttgcagacg tattcctgaa gggtcgccag tgaacccatc ttctaacata
     2401 atttcacaac taatcgtata ttgtttccct tctgctaccg gcacttgcgc tttgctagcg
     2461 tacttcgata gttgatttgt gccatctaag gtaaacacaa tctcatcatt atcatccgcg
     2521 actgttgcgc cagtacctaa agaaaaactt gaagcattaa gtttagcgct aaggttcgga
     2581 ttccccgaat aatcatattc cccgaa
//
LOCUS       DACCXO010000187         2582 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.195, whole genome shotgun sequence.
ACCESSION   DACCXO010000187 DACCXO010000000
VERSION     DACCXO010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.195"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            1..396
                     /locus_tag="IVY70_002793"
     CDS             1..396
                     /locus_tag="IVY70_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959876.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            721..1338
                     /locus_tag="IVY70_002794"
     CDS             721..1338
                     /locus_tag="IVY70_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7959877.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1379..1678
                     /locus_tag="IVY70_002795"
     CDS             1379..1678
                     /locus_tag="IVY70_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959878.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1712..1879
                     /locus_tag="IVY70_002796"
     CDS             1712..1879
                     /locus_tag="IVY70_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7959879.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1924..2529)
                     /locus_tag="IVY70_002797"
     CDS             complement(1924..2529)
                     /locus_tag="IVY70_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7959880.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          898 a          392 c          479 g          813 t
ORIGIN      
        1 atgatgatta tggaagaaat agaatggaat aatctgactc aaaataataa aaataattct
       61 ggatgtggaa ttatctttat gtttgttata gttttgggaa tagtagtact tttaataact
      121 gtcaagatag tagattactt tgcgaatact cctgaagaac aaatacagag aaaacttcct
      181 gagtttgaaa gagtaccacc ttatgatcca agagataatt catttcaagt aaaaaaatta
      241 aaagagtctc caaatgaaat gtggtttgaa gttacagttg aatcaagcga cttcaaagaa
      301 aaaaagaaag aatacaatta tattgtaaag gcaaaagtat caaaaagatt tatttttaac
      361 cctaaattag aaatatatag tgttgtaata gaataatagt gttaaaaaaa ttgtaaaagt
      421 taaagaagtc aaattctgat gtgaaaaata aaatatacca tggaagacat aaaagaaaaa
      481 ctggataact agtttattta atggcagacc gttaaggcct gccattttta tgtattttag
      541 cggaataaat ataatcatgc gggagtgtaa acttactgtt tcaaaaagta tactttttga
      601 catgccttga ttttctttgt ttatgtatgg cataataacc acataataca atttgaaacg
      661 tttctgattt aatctgattg ttttgaaaca attttagtga aaaaagggta gataaaaacg
      721 atgagaaaat ttttttatat ccgggtcagt agttatgatc aatcgatcga acgacaactg
      781 atttccgcga aggaattagg aatccctgaa gaatatatat ttgtagaaaa agccagcggc
      841 aaagatttta aacgccctga atatcaatta atgaaacgga tgtttcgaaa aggagattta
      901 ctctatctac aatcattaga tagactaggc agaaataaac aaatgatttt agaagaatgg
      961 cgagaattaa ctaaggaaaa gcagattgat atttctgtgt tagacatgcc tctgttagat
     1021 actgctagtt acaagcaact agatgggtta gagacgctta tctctgactt aattctgcaa
     1081 ttactcagct atatggccga agacgagcgg aaacgaatta atagccggca aagagaaggc
     1141 gtagaagctg cgaaacaaaa gggtgtaaaa ttcggcagaa agaaaattgc tttacctcct
     1201 gaatttcctg cgatctattc tgactggaaa gcaggaaaga taactgcagt ctatgctatg
     1261 gatcaattgg gcatgaaacg aaataccttt tatcgacggg tgaaagaata tgaacatacc
     1321 ttgcaccact tttcttgata gatataacag ctattagacg ggaagggaga gtttgaaaat
     1381 ggacttaacg aatcaaaggg tcgtttttac tggaacgctg cagcaaatga cacgaaccca
     1441 agccatcggt ttggttcact ctttaaacgg ccattgtcaa tcaagtgtta cttcaaaaac
     1501 aaatttcctg gtttgtggtc agtattctaa gtcgttgttt gatgattctc tctatacaaa
     1561 aaaggaacaa accgcgcggg acttgattgc gttaggtcat gagattacca ttttatcaga
     1621 agtcgaattt tatgcgttaa ttagtcaaca gttgaaagaa tggcaacggt atttataatc
     1681 gttagtaaaa tttacaagga ggaaaatttt tatgaagcga aaatcttatg atcgttgggg
     1741 cattgaaata actagtttta atcgaaatat tgcttttcac gtggagtgtg actgtgggga
     1801 attagcaaaa agtgtttatc aaaaaaatta cttttactgt cctaaatgcc atcgccgata
     1861 cggggtacat ggaacataga aacgaaatag tgcggtagaa aagcgaatga atgtagtata
     1921 tttctacaaa tcattcgctt tctttatttt ataaatagtt ggtcgagaga tcccgttttc
     1981 ttttgcaatc tctgcaactg gaatgccttg ctttaacatg tccaccacgg ttttaaaaac
     2041 taagcgtttt tgcggatctg ttgagttaat agaatactcg attggtcgtc ccttatatcg
     2101 gccggctttt tgagccaata atactccttg ccgctgacgt tcacgaattt tctcacgctc
     2161 gttttgtgca atataactta gcagtgataa aaacatatcg aacatagcgg tatccaggag
     2221 ttcatttcca gtattaaaat ctaaaaactg tgcatctaat atttttaacg ctactttttt
     2281 ctgtttcata aaagcaactg tattttttat atcttcataa tctcttccta aacgatctag
     2341 tgatgtaacg atgacgcaat caccttcacg gatataattt agtaattttt gaaactcagg
     2401 acgctcagta tctttaccac tggctttttc ttggaataat ttatcaatgt taagtccttc
     2461 catcattgcc aattgtcgat ccaaattttg atcttgtgaa gatactcgaa tatagccaat
     2521 ttttcccata aaaaactcct ttttaaaagt gatataattt acatgtaaaa tttacttgta
     2581 gt
//
LOCUS       DACCXO010000188         2581 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.196, whole genome shotgun sequence.
ACCESSION   DACCXO010000188 DACCXO010000000
VERSION     DACCXO010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.196"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            10..366
                     /locus_tag="IVY70_002798"
     CDS             10..366
                     /locus_tag="IVY70_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7959881.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVDNGRYVITHGFTKKTEKTPITE
                     LKHAKELREEWFTNDD"
     gene            356..622
                     /locus_tag="IVY70_002799"
     CDS             356..622
                     /locus_tag="IVY70_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7959882.1"
                     /translation="MMIKKLIEEKKKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            781..1059
                     /locus_tag="IVY70_002800"
     CDS             781..1059
                     /locus_tag="IVY70_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959883.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYVNNSSGQSVINKQIVENRVLLQSFKNKISFCIISSF"
     gene            complement(1056..1737)
                     /locus_tag="IVY70_002801"
     CDS             complement(join(1056..1703,1705..1737))
                     /locus_tag="IVY70_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP7959884.1"
                     /translation="MNHFKGKQFKKDVIIVAVSYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLDYLWKKKNRQSFYSWKMDETYIKIKGHWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVK
                     YLNNLIEQDHRSIKRRHKLYQSLRTASTTIKGMETIRGIYKKSRKEGTLFGFSVCTEI
                     RILLGIPA"
     gene            complement(1769..1930)
                     /locus_tag="IVY70_002802"
     CDS             complement(1769..1930)
                     /locus_tag="IVY70_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959885.1"
                     /translation="MAYMVARTQKHKVGSLNGLQNHVDRKTKNHTNKDIDITKSGSVA
                     KFSKKSILV"
     gene            complement(2254..>2581)
                     /locus_tag="IVY70_002803"
     CDS             complement(2254..>2581)
                     /locus_tag="IVY70_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959886.1"
                     /translation="NREILAKEMADLKAKEAKIRAKRKQIRQKEMISLAKLYCKKEGF
                     KTPAEALDFLESNKRNHSVNDEVLVSLRKFADGLVWQENGAYWRTNDVKGLTQFMAQF
                     RSENVN"
BASE COUNT          852 a          459 c          440 g          830 t
ORIGIN      
        1 taattgggta tggaaaaaat atcttttgaa acatttaaaa gacccaatgg tcatgatgaa
       61 tttttagaat ggttggaaac attaccaaaa aaagatagtg cgaagctttt acgtacaata
      121 gaagaaacag aaaagaatgg gatgctagta gctcaacggt tgaaatgggt gaaaaaatta
      181 gacactaatt tgtacgaatt aagaagtaag gtaggatcaa acatccaaag agccatctat
      241 ttccatgtgg acaatggaag gtatgtcatt actcacggat ttactaaaaa aacggagaaa
      301 acaccaataa ctgaactcaa gcatgcaaaa gaacttagag aggagtggtt cacaaatgat
      361 gattaaaaaa ttgattgaag aaaagaaaaa agataaagat tttgcagaag catatgaccg
      421 tgaaggaaga aaactcgaaa ctgcggttgc tctttatcat gctcgggagg aagcagggct
      481 aactcaagcg gagcttgcac aaaaagcaca tacaactcaa tctactattg ctcgaattga
      541 gagaggggat aacgtatctt tcgataaact tgatgatatc gccagggcat taggtaaaaa
      601 gctggtagta gaatttgctt aaaagcgagt tgaaaatata tctataatag gctcctaact
      661 aatcaattag tttagtgggc ctttttttac tagatagata aaggttgtac tatagtgtaa
      721 aagacaataa attttatact aaaattactt taaagagaac tagagagaaa ggtacaataa
      781 atgaaaacca taaatcttat aaaagtattt atagcatcac caggtgatat agcagcaaaa
      841 aggaatgaag tagaagaaac catatataga tggaatatag aaaatacaga taatacaaaa
      901 gtgattttga tgcctatacg atgggaaact aatgcgattc ctacttatgt aaataatagc
      961 tctgggcaaa gtgttattaa taaacaaatt gtagaaaata gggttctgct gcaaagtttt
     1021 aaaaataaaa tcagtttttg tattatttct tcattttaag ctggaattcc caataatatc
     1081 ctgatttcag tgcagactga aaagccgaag agagtgcctt cttttcggct tttcttgtag
     1141 attcctcgaa tggtttccat ccctttaatt gtggtggaag ctgtacgtag actttgatac
     1201 aatttatgtc gacgttttat cgatcgatga tcttgttcta ttaaattgtt aagatacttc
     1261 acagttcggt gctctgtctt agtatataaa cccacactct gtaactttct aaaggcggag
     1321 ccaagagaag gtgctttatc ggtcacaatt gctttcggct caccaaactg tttatggagt
     1381 cgttttaaga aagcataggc tgcttgcgta tcccgtttct ttcgtaacca gatatctaag
     1441 gttaagccgt ccgcatcaat tgcacgataa agataatgcc aatgtccctt aattttgata
     1501 taggtttcgt ccattttcca tgaatagaag gattgtctat ttttcttctt ccaaagataa
     1561 tcgaggactt tgctgtactc ttgcacccaa cgataaatcg tagtatgaca aacatttatt
     1621 ccacgatcat ataacaattc ctgaacttca cgatagctta gattgtaacg caggtagtaa
     1681 ctaacagcga caataatgac gtcatttttt gaattgtttg cctttaaaat gattcattac
     1741 tctatcctct ctgtcttttt tctcaatttt acactaaaat agattttttg gaaaactttg
     1801 caacagaacc acttttcgta atatcaatgt ctttattcgt atgatttttc gttttacgat
     1861 ctacatgatt ttgaagtcca tttaacgacc caactttatg cttttgtgtt cgtgccacca
     1921 tataggccat aactgtgtac ttcctttcgt taaaattatt gcgagaatca caagctaaat
     1981 tcggaaaaac ctccatgtaa ggtataactt actagactta tactgcgtaa agtctgtaag
     2041 ctctgccgag ggctatatcg gcttaacagc cgatgagaaa ctccgtttct ctcaaacgaa
     2101 aaagcgaacg aaaaacgact gccttcgcat tcctttttta cgtccttttt tcgttcaaaa
     2161 ccattggctt tttgcgtcat tgttttcaca atttttattc cgcaaaaaac caaaagtgaa
     2221 aagtattgct tttcacttca acccctcctc aatttagttc acattttctg aacgaaattg
     2281 tgccataaat tgagtcaaac ccttaacatc attcgtacgc caatatgcac cattttcttg
     2341 ccaaactaat ccatctgcaa attttcttag cgatactaaa acctcatcat ttactgaatg
     2401 atttctttta tttgattcca aaaaatctaa tgcttcagca ggtgtcttaa agccttcctt
     2461 tttacaatat aatttcgcta aagaaatcat ttctttctgt ctaatctgtt ttcgtttcgc
     2521 tctaatcttc gcttcttttg ctttcaaatc cgccatttct tttgccaata tttctctatt
     2581 g
//
LOCUS       DACCXO010000189         2576 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.197, whole genome shotgun sequence.
ACCESSION   DACCXO010000189 DACCXO010000000
VERSION     DACCXO010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.197"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(123..1580)
                     /locus_tag="IVY70_002804"
     CDS             complement(123..1580)
                     /locus_tag="IVY70_002804"
                     /inference="COORDINATES: protein motif:HMM:NF024832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HAP7959887.1"
                     /translation="MGNNIHGKTAKALKWSSLAEIIAKIITPISNMILARLLTPDAFG
                     VVATVTMVISFADMFSDSGFQKFLVQHEFDNEEHMFSSTNVAFTSNLGISLLLWLFIC
                     VFNNKIAHLVGNSGLGLVIIVAGVSLPITSFSSIQSAIFRRNFDYKILCYARIISALI
                     PLVITVPLAIIGLSYWSLVIGSIIGNLVTAAILTKLSEWKPKLFFSKKEFYEMFSFSA
                     WSLVESLGTWLSSYIGTFIVGGILSVYYLGIYKTTMTTVNGIFAIVTSATTTVLFSTL
                     SRLQNSKTEYDKTYLDFIKIVSIFIIPLGIGIYVYRELVTNILLGSQWKEAVPFVGIY
                     GLMSCFTLVLGQYASEYYRGLGKPKANVLMTILHLAVLIPVLIIYSRKGFIELAYGRS
                     FVKIEQIIVFWIILWFGFKFNPFKILKQVWKSCASALLMGVLAKIMLEFCTNYVMQLL
                     SVCLCVILYFFIYSCIFKGKTEIINMVRMFTGKGY"
     gene            complement(1639..2100)
                     /locus_tag="IVY70_002805"
     CDS             complement(1639..2100)
                     /locus_tag="IVY70_002805"
                     /inference="COORDINATES: protein motif:HMM:NF012360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HAP7959888.1"
                     /translation="MRRKAEKWADKACRQYRNGQNKMSKISCYILRIIYGCEFYGQVD
                     FSSNAQLVHNGLGCVFHPKTKIGNNCKIYQNVTLGGNGKIINGKETVGAPTLEENVAV
                     FAGACVLGPINIGHDSIIGANAVVTKSVPPYSLVYGNPAIITELKFDYKFD"
     gene            complement(2111..2509)
                     /locus_tag="IVY70_002806"
     CDS             complement(2111..2509)
                     /locus_tag="IVY70_002806"
                     /inference="COORDINATES: protein motif:HMM:NF016800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP7959889.1"
                     /translation="MLMAGRSGQSLLDSSGRLENYMSSLNAFSDKYLIGYGLGLNNLY
                     TLLGVAVPHNFFIQYLLQTGIIGTLIILSGFFKFTYEELKKSGCYKWLFLMIVVGAMF
                     IPDIVSSRFLYAIVVLCMISASERTIKKES"
BASE COUNT          924 a          483 c          302 g          867 t
ORIGIN      
        1 ttttcaaaag tatttgacgc gttataaaag ctactctttc cattccacca taatacattc
       61 caggaattaa aattgttact tttttcattt tcctaatcac aaccttgcca taaaaatctc
      121 tcttaatatc ctttaccagt aaacattcta accatattaa ttatttctgt ttttccttta
      181 aatatacaac tatatataaa aaaatataga attacacaaa gacacactga aagtaattgc
      241 ataacataat tagtacagaa ttccaacata atttttgcta gaactcccat tagcaatgca
      301 ctagcacaag atttccaaac ctgctttaaa attttaaatg gattaaactt aaaaccaaac
      361 cataaaataa tccaaaagac tataatctgc tctatcttaa cgaaagatct accatatgct
      421 agttctataa accctttcct tgaatagatt attagaactg gaattaatac tgctaaatgt
      481 aggatagtca taagaacatt tgcttttggt tttcctaaac cacgatagta ctcagaggca
      541 tattgtccca agacaagagt aaaacagctc attaatccat atatcccaac aaatggtaca
      601 gcctctttcc attgactccc gagtagaata tttgttacta gttccctata tacatatata
      661 cctataccca gaggaataat aaaaatggaa acaattttaa taaaatctaa atatgtctta
      721 tcatactccg ttttactatt ttgtaaacga gataatgttg aaaataatac agttgttgtt
      781 gctgaagtta caattgcaaa aataccatta accgttgtca ttgtagtttt gtatattccc
      841 aaatagtata cgcttaaaat gcctcctacg ataaaagtac ctatataaga tgacaaccat
      901 gtacctaatg attccactag tgaccacgca ctaaaggaaa acatttcgta aaattctttt
      961 tttgaaaaaa aaagttttgg tttccactct gataacttag ttaatattgc agctgtaact
     1021 aaatttccta ttatagatcc aattactagt gaccaataac ttagtccaat tatcgccaac
     1081 ggtactgtaa taactaaagg tatcaatgca gatattattc tagcataaca caaaattttg
     1141 taatcaaaat ttcgtctaaa aattgccgat tgtatactgg aaaaagaagt aattggaaga
     1201 gaaactccag ctactataat tactaatcca agtccagaat ttccaactaa gtgcgctatt
     1261 ttattattaa acacacaaat aaataaccat aataataaag aaatacctaa gttactagta
     1321 aaagctacat ttgttgatga aaacatgtgt tcttcattat caaattcatg ctgtactaaa
     1381 aatttttgaa atcctgaatc actaaacata tcagcgaaac taataaccat agttacagtg
     1441 gctactactc caaaagcatc tggagtcaat aatctagcta aaatcatatt cgaaattgga
     1501 gttataatct ttgcaattat ttcagctaag gatgaccatt ttaaagcttt tgctgtcttt
     1561 ccatgaatat tatttcccat ttattacccc attttgctaa ataaatatag caacctttcg
     1621 catattaaac attacttttt aatcaaattt ataatcaaat ttcaactctg ttatgattgc
     1681 tggatttcca tatactaagc tatatggtgg aacacttttt gtaactacag catttgcacc
     1741 tatgattgaa tcatgtccga tattaattgg tcctaaaaca catgcaccag cgaaaacagc
     1801 cacattttcc tctagcgttg gtgccccaac agtttctttt ccatttatta ttttgccatt
     1861 tccacccaaa gtcacatttt gatatatttt acaattatta ccgatttttg ttttagggtg
     1921 gaatacacaa ccaagaccat tatggactag ttgagcatta gagctaaaat ctacctgtcc
     1981 gtagaattca catccataaa taatccttaa aatatagcag gatatttttg acattttatt
     2041 ttgaccattc ctgtattgtc tacaagcttt atctgcccat ttctctgctt ttcttctcat
     2101 tttttctacc tcaactttct ttttttatag ttctttctga tgcagaaatc atacacaaaa
     2161 caactattgc gtaaagaaaa cgcgaagata caatatccgg tataaacatt gctcccacta
     2221 ctatcatcaa aaataaccat ttatagcaac ctgatttctt taattcttca tacgtaaatt
     2281 taaagaagcc acttaaaata attagcgtac caataattcc tgtctgtaat aaatattgaa
     2341 taaaaaaatt atgtggtact gctacaccta gtagcgtgta taaattattt aatcctaatc
     2401 catatcctat taagtattta tcactaaaag catttaaaga ggacatatag ttttctaatc
     2461 tgcctgaaga atccaaaaga ctttgtccag aacgacctgc cattaacatt actagtatat
     2521 aaggtgtagt tatgattcca cctattagaa ctaaaagatg aggaacacta attcta
//
LOCUS       DACCXO010000190         2479 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.198, whole genome shotgun sequence.
ACCESSION   DACCXO010000190 DACCXO010000000
VERSION     DACCXO010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.198"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            103..315
                     /locus_tag="IVY70_002807"
     CDS             103..315
                     /locus_tag="IVY70_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959890.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(593..2392)
                     /locus_tag="IVY70_002808"
     CDS             complement(593..2392)
                     /locus_tag="IVY70_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP7959891.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT          886 a          443 c          457 g          693 t
ORIGIN      
        1 taaaaaataa aaaaatggat aaaaacgttt tcaaatacaa ggtaatttgt tattataaaa
       61 taaagaaaaa acaaaataaa aataaagaaa agagggaatg aaatggtaaa agcagaagta
      121 ggaagatctt atatatgcaa accgatcgga gtatctcatg aactaatcgg ctgtattgaa
      181 cgtacatata tcaacacggc tgtcgtgtat gttgaaagtt atgaccaacg agactatcaa
      241 cttatcaatg aaagcaagta tcgcatgctc gtaaaattct gtgatattag cgcacctact
      301 gaattggttt cttaaacagt atttttgaga attcgcaaga actcccttat aatataaata
      361 aagacagaag ggagcgagaa gatatgccaa aaagaaaaag ttcagggaaa atgggaatta
      421 ttcttgcagt gatctctgca attcctgcta ttttagctat tgttcgtgcg atgaaggaat
      481 caagagaaag ttaagcacac attatgagcg tcaaacaaaa aacgcatata cctgcggtgg
      541 caggttatgc gttttttgtt gtataaagac ttttttcatt aacgattttt gattagccga
      601 ctcccaaaac aaaatttagc agtttatctg aataagtgat ttcttcatct gtttgtttca
      661 cactttcatt tagtagtccc atctcatcgg ttatgatacc atccacacca taaaacatca
      721 tgcgactcat ggcatctgaa tcgttgattg tccatacata tacttctttt ccgtcttggt
      781 gggcagaatt aatgaagtta cggttgatcg tactatattc cattgtcaga aaatcagcag
      841 gcgtcacagg gggcccaaca atattgaaag gtaagatgta gcccacataa aagtttggtt
      901 ctgcttcttt taaatctgaa acggtttggt acgtcaaact ttgcaaaata tgatggttct
      961 ctaagataac tttgcggtat ttatggacaa atcgttcgac aaggtcatcc gaatcttttt
     1021 tagaggtctt gatctcaata agtaattttt ggtttagctt atgtgctttt tccaaataag
     1081 aatcaaaaga acaaacaggg gcttgcactc cgttttcagt aactgtaaga tttgtcaatt
     1141 ctttcaatgt aagatcctgt ggtgttccat ttacccctgt taagtgacga agatttgcat
     1201 catgcatcac tacgaaatga ccatcttttg tttcctgaac atccatctct acataatcag
     1261 gatgatagtc tctgcttgtt ttttcaagtg cttctaaagt attttgcgcc ccattttggg
     1321 tactcacacc tctgtgagag acagtcactg gttttgtctg aacagctgaa gtcagataat
     1381 ccatattgta aagacagacg ccaataccga aaaaaactgc aaaaagagtc aaaccagtgt
     1441 ttttcagcgc agagaaacgc aagttggggg cttgtgggat aaaccactta gggatctcag
     1501 gcaagaagcc ttcgtcatcc atgaaatcaa caatgatata aaaaatccct acggtagaca
     1561 tcacgatatt gaataataaa atcccttgta aaagcgtcat agcaaacaca gcgctgatca
     1621 atgcatagtc tgggaaaaat tgttcaacca tactttgtgc caaaataaca acgatatatc
     1681 ctaaagaaga aaggagtaaa atcgtaccgc caatcacaat aaattggccc gtgatggcca
     1741 aaagacgcga tttggtcaat gaccagcttt cccgaatggc tgctcgaaaa gaacgatctc
     1801 gtagaatcat ttccggtaaa gcaaaaatca gtcgaatccc aatatagcca aggaaaatat
     1861 aaacgagtaa aaaggaactg acgatgatcc aacgattagc aaaaataaaa tccataatga
     1921 aagcggggat ttttattttt gacagtagat cagaattaaa acttaatccg ctgatcggta
     1981 gaatcaataa aaaataagca aggaaaaaca aaaaagtaat gggtctcact ttcttcagct
     2041 gaagtatcgt caagtgtaag agttgcttca aagagattgg ttctttcttt ttaataaaaa
     2101 aaacactcat caaaagaaaa gtaaattcga aataaactaa cagtaaaatc gtcatcaaaa
     2161 ctacgattaa cgagagtaga accccaggat gttgagaaaa aattgaagga atattgtcat
     2221 aagacaaata gtcaattgcc cctcggtgta aaataaaacg ggtcgtactg ctcaataagg
     2281 gtaaacaaat aaataaaata aagccatgca tcagcaagac atcacggaaa taagcttgtg
     2341 ttccttttaa aaagtcccaa gcattaccta agctacgttt caaataattc atcctatcac
     2401 ctgctcttca cattgtagcg caaatctaat gaaaaaggaa agggtgcagt gtgttttgca
     2461 gaccctttcc cagatttaa
//
LOCUS       DACCXO010000191         2442 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.199, whole genome shotgun sequence.
ACCESSION   DACCXO010000191 DACCXO010000000
VERSION     DACCXO010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.199"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            288..554
                     /locus_tag="IVY70_002809"
     CDS             288..554
                     /locus_tag="IVY70_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959892.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            612..1124
                     /locus_tag="IVY70_002810"
     CDS             612..1124
                     /locus_tag="IVY70_002810"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7959893.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1100..2158
                     /locus_tag="IVY70_002811"
     CDS             1100..2158
                     /locus_tag="IVY70_002811"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7959894.1"
                     /translation="MGGPRGRMISSSDRKQAVELIQEANRNGARLTYACNELNISVRT
                     YERWTREGTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLAD
                     CSIYIASESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPI
                     KGLFYRLYLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGS
                     PMKAATFQVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARK
                     WTKEFVDWYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVR
                     NWTPHQSVALNPLKETES"
     gene            2318..2442
                     /locus_tag="IVY70_002812"
                     /pseudo
     CDS             2318..2442
                     /locus_tag="IVY70_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          888 a          449 c          489 g          616 t
ORIGIN      
        1 ataaattatc taaagagaat taagttttga ttatgtaata agttttggta ctaaaaaaga
       61 cttgatctaa ttagaccaag tcttttgtta gtgttatatt aataacaaaa taaaaataag
      121 aagtcgctca ctccccgacc aaagtttgtg agcaaaataa acagtcgatg tttgtttctt
      181 aattgtatat cttgatatta aacgatttaa gattattctt caagatatat attcgcaaat
      241 gtaattggaa aagcgacttc ttaaacaaaa ttaaggagtc gctttttatg gcaaaaataa
      301 cgaaaaatga gcaagaaaat ttggtggaaa attacacaaa aaaaccggcg tactctaata
      361 gccggttaga cgcacatttc gtgtgcactt ctaatccgaa attgagcttt gacgctatga
      421 cgatagtcgg aaaccttaat aaaaacagcg ctaaaaagct atccgatttt atgagtttag
      481 atccacaaat tagactttgg gatatacttc aaacaaaatt taaagctaaa gcactacaag
      541 accccctatg ttaggctagt tgtcgtatag gataataagt tataataaag actatacgga
      601 ggatgattac catgacaaat aaaaacaatg aacgttactc taccgaactg aaagcgacag
      661 ccatcaagaa aatgatgcca cccgaaaata gggggattcg ccaattaagt caaactttgg
      721 gcattccaga agcgactctt tattcttggc gtaaaaaagc tagaattcaa gggaatacta
      781 cacccggaac gaaaaaaaat gtcgaaaaat ggtcaagtga agataaattt ttaattgtga
      841 tggaatcatt ctcaatgaac caagtagaac ttgcggaata ttgccgaaag aagggcttat
      901 ttaaagaaca aattgaggct tggaagaaaa cgtgcttatc tgcgaatgaa caggaagaaa
      961 atcgaacgag agaacttgca actgaactaa aagaagagaa aaaacaagcg cgtcaattag
     1021 agaaagaatt acgtcataag gaaaaagcat tagcggaagc cgctgctctc ttattactaa
     1081 gaaaaaaagc acacgcgatt tggggggacc aagaggaaga atgatttcat cttcagatcg
     1141 caaacaagct gttgaactta ttcaagaagc caatcgcaat ggggcccgtc tcacctatgc
     1201 ctgtaatgag ctgaatatta gcgttcgaac ctacgaacga tggacgcgtg aaggtacgat
     1261 agcgcatgat caacgccctt tggcgaaacg tcctgtaccc aaaaataaac tgacggatca
     1321 agaacgcgaa aaaataatag aaaccgtttc aaaaaaagaa tttatgaatt tgccacctag
     1381 ccaaattgta ccaaaacttg cggattgctc gatttatatt gcatcagagt ctagcttcta
     1441 tcgtgtattg cgagaaaaaa atatgcaaca tcaccgtggc cgaagtcaag tttcacaaaa
     1501 acgcataccg ccaagtcacc ttgcgacgaa gccaaatgaa gtttggactt gggatattac
     1561 ttggctaaaa ggtccaataa aaggactctt ttaccggtta tatctcatca ttgatttatt
     1621 tagccgtaaa atggtaggct gggagatttg ggaatcagaa gaagctcgtc atgccgaaaa
     1681 acttattaaa cagacggtgc ttaaggaacg tatccaagga cagccgttgg ttttacactc
     1741 cgataatggc agccctatga aagccgcgac ttttcaagtc ttactcgaaa aattaggaat
     1801 tcaaagttct tattcaagac cacgagtgag taatgacaat ccttattcgg aagcggcttt
     1861 ccgaacgtta aaatatcgac cagaatatcc caataacggc tttaaaacaa tagatgatgc
     1921 tcgaaaatgg acgaaagagt ttgtcgattg gtataacgat tctcatttgc atagtgggat
     1981 tcaatttgtg tctcctgcag cgtgtcatga aggacgacat ctcgctattt tagagaaacg
     2041 aaagcaggtt tacgaggacg ctaaaaagcg ccatcctgaa cgctggtcaa aaggtgttcg
     2101 aaattggact ccacatcaat cggtggcgtt gaatccttta aaagaaacag aaagttaaca
     2161 ggagcataat ctttcaagat tatcaaataa ccctcgtcac ccaatagggg aaaaagttat
     2221 tcttccccta ttggataacg aggccaaccg accataggga ggttctgctt tttgataggt
     2281 ggaaaataaa aatacgacaa ctatattgac aaacaccgac catcgtccag taaagagacg
     2341 caataaattc tatcgaagtt tacgcactgc ctctacaacg attaaaggca tggaagccat
     2401 ccgaggatta tataagaaaa cccgaaaaga aggcactctc tt
//
LOCUS       DACCXO010000192         2426 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.200, whole genome shotgun sequence.
ACCESSION   DACCXO010000192 DACCXO010000000
VERSION     DACCXO010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.200"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(118..708)
                     /locus_tag="IVY70_002813"
     CDS             complement(118..708)
                     /locus_tag="IVY70_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP7959895.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
     gene            1231..1392
                     /locus_tag="IVY70_002814"
     CDS             1231..1392
                     /locus_tag="IVY70_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959896.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            1411..1905
                     /locus_tag="IVY70_002815"
     CDS             1411..1905
                     /locus_tag="IVY70_002815"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin"
                     /protein_id="HAP7959897.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
BASE COUNT          871 a          368 c          403 g          784 t
ORIGIN      
        1 tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt gacaaataac
       61 catcagtaaa attacttgtc atactattga taaggagtat ttaattgaag agaatgatta
      121 ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt atagacgaat
      181 ctttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca agtctgtgaa
      241 ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag cttgttgttc
      301 tataaaatct tctttattct gataattttc cgattctaac caaggaaatt gcgcatttcc
      361 ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg ccccacaaat
      421 agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata aatgatgatt
      481 ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt caggtatttg
      541 ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct ctgattggat
      601 agaagcagtt ttgttttgcc atccctcttt ttggtttagt gtgctagtaa tataagcacc
      661 ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt tcaagctctc
      721 ctttactatc tatgacaagg acaacagttt taacatacag acattcatta aatttggata
      781 gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagacag tcaacttaat
      841 taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca ttataacatt
      901 ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat cttaaaagaa
      961 actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc actcagacat
     1021 gaaaacaacc ctagaaatat ataactagtc caatgaaaaa aaagcgaatg ccgtagaaaa
     1081 caaggattct gcgtacattc atatctcatt ttgcgagcat atctgctatt actgtgacta
     1141 aatgttaata tggttaaaat gaccgttata ttttttatat aaatatcgta taattagtat
     1201 gaatatagaa gaaggaaaga aggtgcaatc ttggaaccta aagacaagta tttagattac
     1261 gctagggtca ataaagataa atttattgaa acaatcatca agggaaaaat ccctgatgat
     1321 gaaaaagatg cggtattcat ggctggaagc ccaggagcag gtaaaacaga ggttgcacta
     1381 ggtttagctt aaaattatga caaccatgtt gtgattgatg cggactattt tagagcacaa
     1441 tttccagaat acaatggaaa gaactcaagt gtttttcaga aagcttcttc ttggttagtt
     1501 gaacaatcat taaaatatgt actggaacat ggctattctt tcatactaga ggatactttt
     1561 gctatcctta gtgcagagaa aaatattatt cgagccctta aaaataactt tcgcgttacg
     1621 attttctatg tttaccaaga tcccaaagtt gcctgggatt tcactcgaaa acgtgaacta
     1681 gctgaaggac gtcatgtgcc aaaagaaatg tttatcaatg cgttttttaa gtccagagag
     1741 aatattgaaa aagtcaaaaa tagacatcct gaagtagtcc ttcacataat gattaaggat
     1801 tatcaaaacg acatttctga agtgcattat gcagcagata atattcaatt agtactacca
     1861 atacaatata cttcaaaaga gttggaggag gaattacatg actgagaaag aacaagtaca
     1921 acaaatcgtg aagaagtata ataaaagtat cgctgattta tcagaaaacg cctcagcgaa
     1981 agaatttaaa acagtaatga attacgtagc agatgaagca aatcgtaagc agcgaaaact
     2041 agttgggttg gataaataaa ttttttaaac catatccttg taaagttgag ttttttattt
     2101 taatgaatta agactattaa atagataatt attgtaactt aaaaaacaca gctaaaaatt
     2161 agttgtgttt tttattaggt ttacttttct ggtataaaag gaactagcta ctttttcaaa
     2221 atttctaaat cattcaatat gtagcgctta ctaaattatt cgccataaaa ttttataatt
     2281 ggatcactca ttgttttacc taagtcatat ttaagtaata accggtgttt ttacataaga
     2341 agacttcggg tattttttag tttgattagc tctgatatac ctataaagaa tatgctaagg
     2401 taagaatagt aaaaattcac atagat
//
LOCUS       DACCXO010000193         2402 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.201, whole genome shotgun sequence.
ACCESSION   DACCXO010000193 DACCXO010000000
VERSION     DACCXO010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.201"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            207..2069
                     /locus_tag="IVY70_002816"
     CDS             207..2069
                     /locus_tag="IVY70_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7959898.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..2401
                     /locus_tag="IVY70_002817"
     CDS             2069..2401
                     /locus_tag="IVY70_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7959899.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          867 a          353 c          466 g          716 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
     2401 at
//
LOCUS       DACCXO010000194         2392 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.202, whole genome shotgun sequence.
ACCESSION   DACCXO010000194 DACCXO010000000
VERSION     DACCXO010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.202"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            232..831
                     /locus_tag="IVY70_002818"
     CDS             232..831
                     /locus_tag="IVY70_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7959900.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLRDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFPEQ
                     LQAQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTAVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            880..1596
                     /locus_tag="IVY70_002819"
     CDS             880..1596
                     /locus_tag="IVY70_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HAP7959901.1"
                     /translation="MNEEKQPNFPDKYHLSRKESVYLLKKNIVELVYNAGKFEGLNTT
                     LLQTEGIIKYNRANNVAVDDVLTVVNLKRGFEMLLNDVQEPLLETSKRINRIVAAEDA
                     LFPGEIRTGGVELSTIQGRYVPPMLTEDEVNNQYGEIMNQEISDTEKALRLFLFISKN
                     QIFWNGNVGTALLIANKIMFSNGLGLLSIPENVFMKFNELLSKYCNSNLLVNESNIIS
                     FLYEQCIFGINYLKIIQHTI"
     gene            1610..2389
                     /locus_tag="IVY70_002820"
     CDS             1610..2389
                     /locus_tag="IVY70_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002396205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAP7959902.1"
                     /translation="MMTIDEAKQLATYFSVGSAGYILDFTNVTWNIFTKKIVGIEIQN
                     HYGGSKGVSFQKFLLDDQFDDSLKNRLIQSLKGIMDRDKKYESTEMNPIVQLLFEEVF
                     KEDTSTALRKMKFSSEIDLLYIRQLPIRIQTDIKNKDFDSVLTKANTLIDEVLKFIIE
                     NTEGPSVNINLFKSKELRKEVFGRLNIKTEKDMDNRIKALVGALNTLSDKILEMRNSQ
                     GDAHAHGSNRIVIKEEEAILVANSSMVLCEYVFTKYKGLNR"
BASE COUNT          858 a          359 c          474 g          701 t
ORIGIN      
        1 aaatttttta aaggggcaat attaatgttc tgagataatc aaatcttacc gaaaatatca
       61 aaagtccaat gtgacacaat tggattttgt cacattcgtt aaaatcgctc catccttaga
      121 ggctctaata ggaaatcact tttcttttag cgcctttttt ctatagcttg tttaaatgtg
      181 acttattatc tgttataatc gtattaagta acatttgaga ggtgtctaaa aatgaaaagc
      241 gtggaaccga ttcgcgacaa aaaaaagatc gatgccatga aggccatttt ggcttccgga
      301 aaatatgggc aacgaaattt ggtgcttttt tcgatcggga tcaatacagc ctatcgaatc
      361 tccgatttaa gacagttaaa attaagggat gtgctagaaa tttcccgtgg gcgggtgatc
      421 gtcaaagaac gtctggccat gaaggaacaa aaaacggcca aacacaattc ggtcttcatt
      481 tcgaacaaat tgcgaaaagt gattcttgat tatgttcaat ccgaatttcc tgagcagttg
      541 caagcacaag acttcagcaa gtatttgttt ccaagtcgaa aaggagcaga tacgcctttg
      601 acccgacaaa gcctttggcg gatcattcat gaagcaggaa ctgcagttgg gttaaaagaa
      661 attggtcccc attcgatgcg taagaccttt ggctattttt tatacaagca aggaaccaaa
      721 acagagatca ttcagtcgct gctaaaccat tcttcacaac gggagacctt acgctatatt
      781 ggaatcaccc aggaagacaa agatactgca gtaaagagtt tggatttatg acagaataat
      841 gccctatata atagaaggaa aaattcaaga ggtgagaaaa tgaacgaaga aaagcagcct
      901 aactttcctg ataaatatca cttatctcgc aaagagtctg tgtatttatt gaagaaaaac
      961 attgtagaac tggtgtataa tgcggggaaa tttgaagggt taaatacaac gttgttacaa
     1021 acagaaggga ttattaagta taatcgggcc aacaacgtgg ctgtagatga tgtcctgacc
     1081 gtcgttaact taaaaagagg ctttgaaatg cttttaaatg acgtacagga gcccctgtta
     1141 gagacaagta agcggattaa tcggatcgtt gcagcagagg atgcgctttt tcctggtgaa
     1201 atacgaaccg gtggggtaga attatcgacg attcaaggaa gatatgtgcc accaatgtta
     1261 actgaagacg aggtaaacaa tcaatatggt gagataatga atcaagagat atcagatacg
     1321 gaaaaagccc tgcgactgtt tctattcatt tcgaaaaatc aaatattctg gaatggtaat
     1381 gtgggtacag ccttgttgat tgcgaataag attatgttta gcaacggttt aggactatta
     1441 tcaatacccg aaaatgtatt tatgaaattt aacgaacttt tatcaaagta ttgcaacagt
     1501 aatctattag tcaatgagtc aaatattata tcatttttat atgaacagtg tatctttggt
     1561 attaattatt tgaaaataat ccaacataca atttaagagg tataagtaaa tgatgactat
     1621 agacgaagca aaacaattag caacttattt ttcagttggt tcagcgggat atattttaga
     1681 ctttactaat gtaacgtgga acatatttac aaagaaaatc gtagggattg aaatacaaaa
     1741 tcattatggg ggctcaaagg gagtatcctt tcaaaagttt ctgcttgacg atcaatttga
     1801 tgattcgtta aagaatcggt taatccaatc tttaaaaggt attatggatc gagataaaaa
     1861 atatgaatct actgaaatga atcccatagt tcagttgctt tttgaggaag tatttaaaga
     1921 agatacttca actgcattaa gaaaaatgaa attttcaagc gagattgatt tactttatat
     1981 tcggcagcta cccattagga ttcaaacaga tattaaaaac aaggattttg actctgtttt
     2041 gacaaaagca aatactttaa tagatgaagt tctaaaattt attattgaaa ataccgaagg
     2101 accgagtgtt aatattaatt tatttaagtc aaaagaatta agaaaagaag tatttggccg
     2161 cctcaatatt aaaactgaaa aagatatgga taatagaatc aaagcccttg ttggagcact
     2221 caatacatta tcagataaaa tactcgaaat gagaaatagt caaggagatg ctcatgcaca
     2281 tggctcaaat agaatagtaa ttaaggaaga agaagcaatt cttgttgcaa attcttctat
     2341 ggttttgtgc gagtatgtgt ttactaagta taaaggatta aatagataat aa
//
LOCUS       DACCXO010000195         2374 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.203, whole genome shotgun sequence.
ACCESSION   DACCXO010000195 DACCXO010000000
VERSION     DACCXO010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.203"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            204..422
                     /locus_tag="IVY70_002821"
                     /pseudo
     CDS             204..422
                     /locus_tag="IVY70_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            682..936
                     /locus_tag="IVY70_002822"
     CDS             682..936
                     /locus_tag="IVY70_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7959903.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            937..1254
                     /locus_tag="IVY70_002823"
     CDS             937..1254
                     /locus_tag="IVY70_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7959904.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1813..2244
                     /locus_tag="IVY70_002824"
                     /pseudo
     CDS             1813..2244
                     /locus_tag="IVY70_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2301..>2374)
                     /locus_tag="IVY70_002825"
     CDS             complement(2301..>2374)
                     /locus_tag="IVY70_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP7959905.1"
                     /translation="RRNGTLFGFSVSTEIKVLMGITA"
BASE COUNT          828 a          349 c          428 g          769 t
ORIGIN      
        1 ttatgtattg atttttctaa caaaagcaca tattttaata tatactacct attttaggta
       61 gtatatattt ttagatacaa tatcgatcag ttttgaggaa atgaaattct agatattttt
      121 tgctacatag agttactttc aatatttttt aacgtaatta ggcagaagtc actcatcctc
      181 tataggtttg tgatgaaatt gctattgatc catttaagta agagcaaaaa actggaggtt
      241 tatcaggtaa ttggtctaat ggatttaagt atgcggtcac cgtgtataga gttgcttatg
      301 agattcaaga aaataaagtt attccagtat tgctatatgg aacacataac aatttttatg
      361 aattagccga gaacactcgc aactttattc gtgtagatga atcggcttcg gttgagttat
      421 aactttagct atatcaatca accgaccgac atatgttaaa tctatatata ataaaaatac
      481 ctacttaaat ggatgttaac ggaagtgtat ggtgagtagt tcactcgtat tttgatatcg
      541 tttgtatatg ttttcagcgt tctaagtggc ttattttgtc caataataat atggagtgga
      601 atcccttaag aaacaccgtt ggcaattgtc gttataacat gttataattt atttatcaaa
      661 aactaaaaga agtgtgataa catgggaaaa gaactaaagg ttagaaagat cggcaatagc
      721 gtgggagtga tccttccaag ctctcttgga ttaaaaagcg gagacactat ccaagccaaa
      781 caagaaggca acttattcat attagatact actcaaatag caaaagaaca cgatcgcaaa
      841 ttgattgaag aaagtttcca agactttgag aaaggtttaa cagtttcaga aattgaaatg
      901 gtaaaagctt tcggaaaata cggctggagt gaatagatga aagagtacac gctagtttat
      961 tctaaaagtt tccagaccag ccttgaaacg ataatagaag aatgggaaaa tgagcttgct
     1021 ctttctccag ccagcataaa aagatttgta ggtgcgatcc agaaggcttt agaactaacc
     1081 aaaaattttc caactatgca tgaggaagtt tccgaaatct atggaatgga tatcccaaca
     1141 tatcggatcg tgatcggcaa aagctatgcc ttattttatc ggatcgacca tgaaaaaaaa
     1201 gagatacttg taggacgtat tttccaatct aaacaaatga aactaggctt ttaacaataa
     1261 ttaggcaaaa gtcgctcatc ctctacaggt gggtgattaa tgccgctcga tatggttgtt
     1321 aaccgtcggt tactcgaaga ttgatatata agacttagta cctttttatt ttgctccatg
     1381 ttatggccga aggtctaaag atacaatcat tagtctagag aaaaattaag aaccgtagga
     1441 actacgggga tcgcttggtg aataagagac actgctatta gtgaaaaaat aagatatcaa
     1501 gtacgctcta ttcccaaaaa tttcgtgact ttactcatga gaggttcaag tagtatattt
     1561 ttaatcaaaa ttgacgtaac ttttaataaa tactacctaa ataaggtagt atttattttt
     1621 gtgttttaaa agaggaagtc caatatatct atgggaaact tcttaaatat tatgttggta
     1681 catgaaaata tacatccctg ctagactatt tacaacattt tactttgtac gtattcttgt
     1741 tttaaaagga atgggataaa atataggtac aataactatt tgatgctaaa tataattaat
     1801 ggaggagaga tagtgaacat acttaatgaa aaaagatatg tatggtttgc tggtaaaaaa
     1861 tatattacta ctttcaaaag attatccgag ttaatgcagc agactgttaa aagtgctatg
     1921 cctgaaggat acaagtttaa ttttgttcaa tcttcagtat ctactgaaag tatctatttt
     1981 tcaattcttt ctgagagtag gctttttcat tttagaataa gttctcatgt aaagaaaact
     2041 gatgttgata tatatacgtt ccgtacaaat aattataaaa atttaaatga tttgagtaga
     2101 agtatcagtg aatatctcaa ggggaatagc tatctcaaga ttcgattttc acatatatta
     2161 attttactac catatttgaa tgagaatttc accctaattc aagtagataa gtctttcatt
     2221 gcctcaggta atcaaggaaa tatgaaggtt ctgttgcgaa gtttgaaaat caaacgcgcc
     2281 ctctctgaac tccaaatatc ttaggctgtt attcccatta ataccttgat ttcagtagac
     2341 accgaaaagc cgaagagcgt tccatttctt cggt
//
LOCUS       DACCXO010000196         2359 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.204, whole genome shotgun sequence.
ACCESSION   DACCXO010000196 DACCXO010000000
VERSION     DACCXO010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.204"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..981
                     /locus_tag="IVY70_002826"
     CDS             <1..981
                     /locus_tag="IVY70_002826"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012744.1,HMM:NF024831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7959906.1"
                     /translation="IYQVDFYRNPLNLKNIKAYHQIVEIIKNEKIDIIHCNTPIGGVV
                     GRIAGLKCKVKKVIYQAHGFHFYSGAPKINWFIYYPMERWLAHYTDVLITINHEDYTL
                     AQKLRLRKGGKAYYIPGVGIDTNRFMSSTSIRNFRESICKELNIPDTAKIILSVGELN
                     ENKNHLSVIHALQKIPLHTDIYYIIAGVGEMRETLQNIANELGLHQRVFLLGYRSDIP
                     KLLAAANIYILPSLREGLNASLMEAMASGLPCIVGNIRGNVDLIEDGKGGFLINPKDE
                     EEIAGAINRLADNKLLCESMGAHNLDKIKDFDLSVVINQINNVYHEIVSK"
     gene            1004..2056
                     /locus_tag="IVY70_002827"
     CDS             1004..2056
                     /locus_tag="IVY70_002827"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012744.1,HMM:NF024831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7959907.1"
                     /translation="MKKIKIMHLLQSNRFSGAENIVCQIMSLFQNNEKFEMIYVSPDG
                     PIANILNIRGLSYLPLKKFSRKEIDRAVHELNPDIIHAHDFNASVRASRYKKQIILSH
                     LHNNPLWLPKLDIRTIIYSLCSSRFNSVIGVSNSVKEEFRYKQLIKDKFVLLPNTVNT
                     ERIIKMGNEFVEDDIDILYVGRMAEPKNPIGAINVLENVVKKYSKHLRVVMIGEGPLF
                     DEVKMQIQKRNLTDVIELKGFKSNPYKYMKKSKVLLMPSLWEGFGLVSVEAMLLETPV
                     VAYNVGGLKDIIDETNGFLCGSEVELSNSLMKLLNSEELRKKMGENAAISAKKFSNMN
                     RYQGVLEKIYVSAALK"
BASE COUNT          878 a          281 c          416 g          784 t
ORIGIN      
        1 atataccaag ttgattttta tagaaaccca ctaaatttaa aaaatataaa agcttatcat
       61 cagattgtag agattataaa aaatgaaaaa atagatatta tccattgtaa tacacctatt
      121 ggaggtgttg tcggacggat tgctggtctt aaatgtaaag tcaaaaaagt aatttaccag
      181 gcacatggat ttcattttta ttcaggtgct cctaaaataa attggtttat atattatcct
      241 atggaacgtt ggctagctca ttatacagat gttttgatta caataaatca tgaggattat
      301 actttagctc aaaaacttcg tcttaggaaa ggtggaaaag cttattatat tccaggtgtg
      361 ggtatagata caaataggtt tatgtcttcc acatcaataa ggaattttag agaaagtata
      421 tgtaaagaac ttaatatacc tgatacagca aaaatcattt tatctgtagg agagctaaat
      481 gagaataaaa accatttatc agtaatacat gcattacaaa aaattccgct gcatacagat
      541 atctattata ttatcgctgg ggtaggagaa atgagggaga cactacaaaa tattgctaat
      601 gaattaggat tacatcagcg agttttttta ctaggatata gaagtgatat acccaaatta
      661 ttagctgctg caaacatata tattctaccc tcattaaggg aaggcctcaa cgcctcttta
      721 atggaagcga tggctagtgg tttaccatgt attgtcggta atataagagg aaatgtagat
      781 cttatagaag atggtaaggg tggatttcta atcaatccta aagacgaaga agaaattgct
      841 ggtgcgataa atagattggc tgataacaaa ttattgtgtg aatctatggg ggcacataat
      901 ttagacaaga taaaagattt tgatttatca gttgtgataa accaaataaa taatgtttac
      961 catgaaattg taagtaaata attgaattga aagtgatatt aatatgaaaa aaataaaaat
     1021 tatgcatttg ttgcaaagta atagattttc tggcgctgaa aatattgttt gtcaaataat
     1081 gagtttattt caaaataacg aaaaatttga gatgatttac gttagcccag atggtcctat
     1141 agcaaatatt ttaaatatta gaggtttaag ttacttacca ttaaaaaaat tttcgagaaa
     1201 agaaattgac agagccgtac atgaattaaa cccagatata attcatgctc atgattttaa
     1261 tgctagtgta agagcaagta gatataagaa gcaaatcatc ctttcccatt tgcacaataa
     1321 tcccttgtgg ttacctaaac tagacataag aactattata tattctttat gttcgtctag
     1381 gtttaattca gtaattggtg tttcgaattc cgttaaggaa gaatttcgtt acaaacaatt
     1441 aataaaagat aaatttgtgt tgttacctaa cactgtaaat actgaaagaa ttataaaaat
     1501 ggggaatgaa tttgttgaag atgatattga tattttatat gtaggacgca tggcagaacc
     1561 taaaaatcca ataggtgcaa ttaatgtttt agaaaatgta gtaaaaaaat attcaaaaca
     1621 tttacgtgtc gtgatgattg gtgaaggacc attatttgat gaagttaaga tgcaaataca
     1681 aaaaagaaac ttaacagatg ttattgagtt aaagggattc aaaagtaatc cctataagta
     1741 catgaagaaa tctaaagttc tattaatgcc atctctttgg gaagggtttg gcctagtatc
     1801 agtagaggca atgttgttag agacgcctgt tgtagcttat aacgtaggag gattgaagga
     1861 tattatcgat gagacaaatg gatttctgtg tggtagtgaa gttgaattat ctaattcact
     1921 aatgaagcta ttaaattcag aagaacttag aaaaaaaatg ggagaaaatg ctgctattag
     1981 tgcaaagaaa ttttctaata tgaatcgtta tcaaggagta ttagaaaaaa tttatgtatc
     2041 tgcagcttta aaataaaata tgaattataa aaatagagat caattagttt tacgaaaaat
     2101 aatacccatt agtattttgt ttcaatcagg aatgattttt tattcactga tgccctcaat
     2161 agcaattgca gttgtttctt tttttatgtt tttgtattat atgtggtgct taaaaaagta
     2221 tttttgttta gcagatagct tattgttaac atcaattctt ttcattccaa catcaactat
     2281 atctatatta ggaactagtt attctagctt accattgacg tggcataatc taataataat
     2341 aattttgatg ttatatatt
//
LOCUS       DACCXO010000197         2303 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.205, whole genome shotgun sequence.
ACCESSION   DACCXO010000197 DACCXO010000000
VERSION     DACCXO010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.205"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            89..1390
                     /locus_tag="IVY70_002828"
     CDS             89..1390
                     /locus_tag="IVY70_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7959908.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(1494..1694)
                     /locus_tag="IVY70_002829"
     CDS             complement(1494..1694)
                     /locus_tag="IVY70_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7959909.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            2084..2239
                     /locus_tag="IVY70_002830"
     CDS             2084..2239
                     /locus_tag="IVY70_002830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959910.1"
                     /translation="MAMTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAV
                     QELKKSA"
BASE COUNT          748 a          441 c          400 g          714 t
ORIGIN      
        1 atcggtccct tttgagcatg aaaaaacaaa acatacagaa gctccgttag aattgaatca
       61 ccacaacaca atcaaaggag cgttctgtat gcctgatctt catcatatca aactactact
      121 tggaatcaaa gataaaaaca ttttcatcac aaatgtggaa tcgaagagca ttaagaatat
      181 caagagtctt gttgtttctg cgacgttaag taaagaaatt cgtcgctgtc ctttgtgtaa
      241 acagatgaat catgaaggca tgatcgttaa gaacggaaag aaaaaatcgc tcattcaact
      301 taataaatgt gcgaaccaac tgacttatct tgctttagcg aaacaacgct accactgtcg
      361 tggctgccac acctatttta cggcgaatac ctacattgtc gatagaaact gctttatcgc
      421 taaacaagtg cgctataaaa ttttagaaga gctaactgaa aaacaagcaa tgacgacaat
      481 tgctaagcat tgcggcgttt cttggtcgac cgtttctcgg acactcgctt ctctgatccc
      541 catgacaaaa gtaaaaagaa attggttacc gcgttgttta ttaatggatg aatttcgttc
      601 actgaaaaat caagtcggtc cctactcctt ttcttgtatg gatggcgata ctggaaaatt
      661 actggatatt cttccttcac ggaagaaaaa ggctctcgtt tcttatttta tgcaatttga
      721 acgacgcgca cgattgaagg taaaggtcat cgtgacggac atgaatgttt cttatgcctc
      781 tctgataaag gaatgttttc caaaggcaaa actcgttgtg gatcgttttc acattgtcaa
      841 acatctcatt cgtaaatttg aggatattcg cgtacgaatc atgaaaagct ttgatcgaaa
      901 cgatccaaca caagccaaat attatcgaca attaaaggcg ttgtctcggc ttctgatcaa
      961 acgacaggac aagcttgttt atgacaaatg gaccaaatgg cgcaattttg gttgggcgta
     1021 tctcaccgaa agtgaagtgg tggatcgtct gctttcgatc tctgacgaat tacgaatcgc
     1081 ctatttttac tatcaagaaa ttcttcaagc gtttcacgac aaagaggcgg acactttctt
     1141 taaacttgta agaacgatgc ccaattccgt tccaaaagaa ctgcattcta taaaaaaagc
     1201 ttttataaac tatgaatccg gcattcgttt agccctcgaa ttgccttatt caaatgccaa
     1261 aatagaaaat ctgcataccc acatcaaagc attgaaacga gtcgcttatg gctttcgaag
     1321 ctttcggaaa atgaagacca gaattttctt actaaataat ctgattacct atgaatcaaa
     1381 aaacatctaa agtagaaaag gaattctacc ttagatgttc ttatctcact ctatcagtcc
     1441 tatttgacaa agagcccttt tctttcgaaa agacggttaa aagtcaattt ttattaagct
     1501 ttaacaatgt ttgttgcttg agggccacgt tgaccatctt caacatcgta agacactgct
     1561 tggccttctt ctaaagtttt gaagccgtca ccttggatag ctgaaaaatg tgcaaataca
     1621 tcatttccat cttcaccagt gataaagcca aaacctttgt ctgcgttgaa ccattttact
     1681 gtaccgttat tcataaataa attcctcctg atacgtatca atgtacgttt tttcgttgca
     1741 ataaatattt gataggcaaa aggagtttta tctatgtaga tatatcgctc aaattacgtt
     1801 tcaaaataga ttactcattt aatatatcat gctttttcta ataacacaag gataatgcaa
     1861 tgaaatataa aattcattgt cttctattgt cttcgaatcg aagaaaaagt tgaataaccg
     1921 cgattgattc actttttgtt ttcttgttta tttgctttta tctgcaactt ttgactttaa
     1981 aatttcttaa ctatgaaaac ctttcaattg gtggtatact tataaatgga atttcgttat
     2041 caacgcgttc gttgcctttc agcaagtatt taaagaaagg agaatggcta tgacattcta
     2101 taacaaagat atacaatatc gagtaacctt ggatacagat ttgaatctat ttgtcgtttt
     2161 tgacaaagaa aataccaatc atgtagcgac aggtgttacg atcgaacaag cagtgcaaga
     2221 attaaaaaaa tctgcataag gagtggaaac cctcatatgg acgattctcg ttcttctctt
     2281 taaacgcatc tactggtaga taa
//
LOCUS       DACCXO010000198         2268 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.206, whole genome shotgun sequence.
ACCESSION   DACCXO010000198 DACCXO010000000
VERSION     DACCXO010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.206"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(1015..1305)
                     /locus_tag="IVY70_002831"
     CDS             complement(1015..1305)
                     /locus_tag="IVY70_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959911.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1513..2204)
                     /locus_tag="IVY70_002832"
                     /pseudo
     CDS             complement(1513..2204)
                     /locus_tag="IVY70_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          647 a          432 c          331 g          858 t
ORIGIN      
        1 catacttgca aagtaatact acaattccta acataatcgt agaatttaat aatttccata
       61 gcataaagtg tttagaaaag aacattaaga atagttctat aataattctt gaactccatg
      121 tcttataatg ttctatcgaa gcacccaaat agccaagttc atctaatcta cttgcataaa
      181 ctacgtcatc cgctctcata gtcggcatat acatattaaa gagtaaaaag ataccccaaa
      241 tccctattag taacaagatc agttctttgt tattcgttat ttttctatta tgtttgttca
      301 tttttatact ccttcttata tttcctaata gagtataaca tagctttaag cattacttaa
      361 agataactta aaagttatct tttaaatact taattgttaa ataccttatt tgatgcaaaa
      421 gaaatctacg atagtcaaca aaaaaatttt cattatatcc agaaaaattt ttcttagtta
      481 aggacctatg gattgcagta atgcagaatt cgtcgaataa actgccaaag acatacaatc
      541 atattgcttt taaaattgac gaacaagata tagattcgtt tgtctcaaaa atccaaatgt
      601 taggtttaac tgtcgaacca ggacgttctc gtgtgaaagg cgaggcttct tcaatttact
      661 tttacgacta tgataatcac ctcttcgagt tacataccgg tactctccag gaacgtttaa
      721 aatcctacaa ttccactacc tgagactagt aatgactagt ctctttttat ggcctctgat
      781 ccccccattg agcgaatact aatagggctt cgaattgatc tgttcaatct ttaagcaata
      841 tccctcaaat gagtttggaa agtttagtga ttcatcacaa atacttaaat acatgtagtt
      901 atatatagat tgaggttctt gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc
      961 atccatcttt ccaaaacaag cggctaggac atttttgtct cagccgcttg ttttctagtt
     1021 tattctttca tcttgtcgga ccttttttct tgggtataga cttttctcat attctaccac
     1081 aattttgggt ttccctaaca catgatagga tctcacaaca aattgctttc tattattata
     1141 ataacctgta acgactaact tcatcccttc gtctacttca gctaggaagt tcaaactatg
     1201 ggaagcaatc aaacaattga ccccatttaa actgaaacgg atcaacggtc tggcactaac
     1261 ttgcaaagta cggatcttat ctactgttcc atattttttc tccataacct tcacctcaat
     1321 accattatac gaacatacat tccctttgta aagaagaaac tctgttttat ctttttgact
     1381 ttttgtctat tacctttctt aaaatcatat tggcacttcc tgcgaagatg aaggacatgt
     1441 actaaaaaaa agaggcagca gtatagatta ctgttgcttc tttactaatt taggtttact
     1501 tgttaagaaa gattatttac gcaaatgttc ttcaacaatt tgctgagatt tatcagctgt
     1561 atcttccact tcatcttttg ctttttctaa cttttgaata tattgtttca agcttgagtt
     1621 ttcagcagtt gcttcgtcta atttcttttg tgtatctgtt aactgatttc tacatttgtt
     1681 ttttcttgtg tcagtgttgc gatttgttga ttcaaaccat ttacagtatt ttgtaattct
     1741 gaaagttttg cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag atgattgatt
     1801 tcatcatttt tttggttgat ctcatctagt ttgacattga tttcattttg tttgctttgt
     1861 agttgtgcat ccttgctgac taattgactt tctaagtttt gtttttctgc tgccgattga
     1921 tttttttggt tttctaattc atttactttt gtttggtatt cttggacatc tgcctttgaa
     1981 tcttgcaatg tctgttcttt ttgtgctagt cgagcggtaa gatctgtaat cgcttgctct
     2041 ttatctgtca atgtatgatc aagctgtgtc agattctcat tgattgtatc aatatctgtc
     2101 tctccacccc acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat
     2161 agaaatgcaa cgacaatccc accagtaata aatttctttt tcatttgcat agctccttaa
     2221 tccaatactt ttacatgcta aaagtaagtg atctcaaaca caatgtca
//
LOCUS       DACCXO010000199         2256 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.207, whole genome shotgun sequence.
ACCESSION   DACCXO010000199 DACCXO010000000
VERSION     DACCXO010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.207"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(150..2147)
                     /locus_tag="IVY70_002833"
     CDS             complement(150..2147)
                     /locus_tag="IVY70_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP7959912.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          573 a          504 c          411 g          768 t
ORIGIN      
        1 atttttgtca caaccttttt tgccgagtaa acggtgttca aaagccagtt tctcggtatg
       61 aagtcaaatt taagcaaaaa tgtgagaagc tattttcaca aaatttttct tcatactcga
      121 aactaagcgg ctttttcacg acctcttttt tattttgcca tgaacatatc ataatacgcg
      181 tccattattt ctttagcaat cgtcaagttt atgtggtctt gttcgtctaa taagttcggc
      241 aagacaacac tgatggcaat ttccggattg tctgttggtc cgtatgccac gatattacta
      301 ttgactgtcg caatatctgg atgaccttcc gcaaccgttt ctgcagtccc tgtttttgcg
      361 gaaaggtcca ttttcgcact ggctaatggt ttggctgttg tgtaaggatc ggtaccatgg
      421 acaacttggt ggaatccttc acggaggatc gccatatttt ctgcagaaat attgaccgta
      481 ttttctactg ttgtgccaat ttctttcttc acttcaccca agtttccatt ttcgtcattg
      541 ccatagatcg ctttgactag gtgcggctgt actcgtttcc cgccattagc gactgttgaa
      601 gcatattgag ctaattggat tggtgtatat gtgtcaaact gtccaaatgc caaatcggta
      661 aagttacctg gagcatacca ttctttgcca ttatccgcat tgaacttctt catataatca
      721 acagaacgtg agatcccagg agattcattc ggcaaatcga tcccagtcgt tgtaccaaga
      781 ccaaactctt tgaaagagtc tctcaattgc tgataagctt tagcttcttc atctaaacta
      841 ggtagtccca ttccaggtgt atagtctagt cctaacatct ttaacgccac tttgatcata
      901 tacgtattag aagacaattc cgatgctttg actgcatcaa gagcaacttg tccgcttcga
      961 ttaaagacag agcttttttc tgaagcacct tgcagacgaa tcggctcatc aatcaaaact
     1021 tgatttccgc tgatcgcgtt acttgcccag cctgctgtca aagtaccagc cttgactaca
     1081 gaccctggat caaaggcgct agtaatcgtg cctaaagcat tctcggtgat ttctttggaa
     1141 ccttgttcat gactgaaacc agtcattgct agcacttctc ctgtctgagg attcatagcc
     1201 actgcataag caccaggcga atactgtgct ttgccgttgt tgatcaatgt ttgataattc
     1261 cttttcaaaa tttcttctac ttttgattgg aattctgcat tcatagataa cattaaatta
     1321 gaaccttttt ccccagagaa gatttctttt tgattcgaaa catttccttc attatcaagg
     1381 gaaacttcat attgggtttt ggttccttgc aggacatctt catattgttt ttccaaataa
     1441 ctttgtccta cacggtcatt ccgagaataa ccttttgcta ggtatttctc tgcttcatct
     1501 gctggaagtc cttgcttttc agtcgtcaca ctgccaagaa tacttttcag tgaacttgct
     1561 gcattatatt ctcgtgtcca gtctgtgcca gtcgaaactc ctggaagttc agaagcgcgt
     1621 tctgccacaa cagctaactc ttgatcagta acaccagagt ttttgatata gacagtgttt
     1681 aattcatatg cactattcat ccgtttaaag atggtagcaa tttttaattg ctcctcgttt
     1741 aaattaatct catcagctgt gactttattg accatcgcac tatattgttc gctgttccct
     1801 aattgctttt cttttgccga aagtcttttt gtggcttctt tcaaatgttt gggatcagcc
     1861 agccaaaaat cttttttatc tctatctgtc agattttcat ctaccggaac atcgatcagc
     1921 gagttcagct tcgttgcgat ttcaagcaaa tctttagccg taacttgtgt tcctctcgta
     1981 tatgtgatgg cctgattcgc tttattcgta actaaagctt ttcctgtgga gtcgtagatc
     2041 attccgcgag gagtactttc ttggaccgta atcgtagaag tagacttcac tcgcgcctcc
     2101 atctcttcgc cgttcacgat ctgcaaataa cccaatctag caatcaacgc aacaaataac
     2161 gtaaatataa tgaaaaaaag aaaatttaag cgaaaaggaa catgggaccg gcgaaacgtt
     2221 ttgttcttag gttcttgtgc accttttctt attttt
//
LOCUS       DACCXO010000200         2246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.208, whole genome shotgun sequence.
ACCESSION   DACCXO010000200 DACCXO010000000
VERSION     DACCXO010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.208"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(142..1290)
                     /gene="tnpB"
                     /locus_tag="IVY70_002834"
     CDS             complement(142..1290)
                     /gene="tnpB"
                     /locus_tag="IVY70_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP7959913.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1307..1711)
                     /locus_tag="IVY70_002835"
     CDS             complement(1307..1711)
                     /locus_tag="IVY70_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP7959914.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            1905..>2246
                     /locus_tag="IVY70_002836"
     CDS             1905..>2246
                     /locus_tag="IVY70_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959915.1"
                     /translation="MIVGASTPLNLFPFSARGIKVEAASSRYNHNHRGFTCYKMNYYV
                     TNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSAVDAMNVFVRAANQGKG
                     VQLTYNAHFSNT"
BASE COUNT          677 a          383 c          371 g          815 t
ORIGIN      
        1 gctaaacctg acggttctta acgcttgaag tgggagcttc ttgggaatag agcatacttg
       61 atagcgtatt tctttactaa cagcggtgtc tcttatttac caagctatcc ccgtagttcc
      121 tacggttctt gatgattgga tctaagccaa tcccatagaa tgtagtctta gaccttcggt
      181 cagaatattg atactagcat tgatatcacg atcatgatga gtatgacaaa caggacaagt
      241 ccattctcga atttcaagag atttcttgcc atctttatgt ccgcactctg aacaaatctg
      301 actagacgga aaccatttat ctactttgat gatttttcgt ccataccagt cagccttgta
      361 ttgtaattta gtcacaaact ttgaccaaga aacatcagaa atacttttag ctaatttatg
      421 atttcgcaac ataccttttg tgtttaagtc ttcaatacag ataatatcgt gatttttgat
      481 catttctgta ctcaacttat tcagaaaatc agtacgttga ttcattactt tttcatgcaa
      541 tctagctact ttgcgttttt gtttttgata atttttagct tcaaatagat tggtaccttt
      601 atttttagcc aataaagcac gcctagacaa tttgcgttgt tcacgcttta gtttcttttc
      661 cattttggac gtgaatttat tattatcaat tttttgtcca tcagaaagaa tcgcaaagtc
      721 cataatacct aagtcaatac caattgtgga attagtttta ggtaattcga taatttcttc
      781 tttacacaac aaagaaatat agtatttacc gctagaatga cgtgatattg tagcagattt
      841 gataatccct tttggttgtc tatgaagctt aattcttact aatgacttca atttaggaac
      901 tttgatgaat ttactatcaa tcaaagcaac tgtaccattt tgattattcg ttgtataact
      961 ctgaacagga tttttcttac ttttgaaacg tggaaatcca acggatttat cccgaaaaaa
     1021 attcttgtat gctttatcta aattaagttg ggcattggct aaagcaaggc tatcaatttc
     1081 tttcaaaaac ggaaattctt tcttgtattt agctggtgtc ggaaaagtca tttttttaga
     1141 agaatcattt ttaacttctt catacgcctt ttttcggtca tcaagcatta gattgtagac
     1201 cttacggaca caaccaaaag atttggcaaa gaagatttct tgttcctctg ttggatagat
     1261 tctgaatttg tatgctttta gttgttccat aaagttcacc tactttctat ttatgattgt
     1321 ttccttgatt ttggatatac ttcttaatca catcaattgg tgcgccacca gttgtcagaa
     1381 gacaaaaact tttagaccag aacatttctt tccaaagaaa ttgtttgact cttggaaagt
     1441 cacgttttat caatcttgag ctggcacttt tataggcatt gatgaatttt gtcaattctg
     1501 ttttaggttg agctttgaac ataatatgaa cgtggtcttt atcatgattc cactccacta
     1561 aagtaatgtg atacgattct gaaattcttt caaaagtagt tttggcataa tcagatattt
     1621 catcatcaat cacttgtcta cgatatttcg ttactaaaac aaggtggtaa taaagaagaa
     1681 atactgaatg attatttgta tctaattcca tgttatatca actcatttct tgtatagact
     1741 gattgtaaca tggaacaaaa taaaaaggca atacgcctta gttgtcggtc ttcgagttac
     1801 caacggtaac cctcataccg aacggcattc atcacccacc tatagaggat gggcgacttc
     1861 tgcctaattc tgttaaaatt attacttgtc tatttttgtt cggtatgatt gttggagcaa
     1921 gtactccgtt aaatctgttt ccttttagtg ctagaggtat aaaagtggaa gctgcaagtt
     1981 cacgatataa tcataatcat cgtggattta cttgctataa aatgaactat tatgttacta
     2041 ataaaatgtg tagagatttg aaaaaaaatt ataataagct aaagactcct gctcaaattg
     2101 catcttttat tcctattggt ggagttggaa catgggttat tacaaatgct tttagtagtg
     2161 ctgttgatgc aatgaatgtc tttgtaagag ctgcaaatca aggaaaaggt gtacaattaa
     2221 catataatgc tcactttagc aataca
//
LOCUS       DACCXO010000201         2195 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.209, whole genome shotgun sequence.
ACCESSION   DACCXO010000201 DACCXO010000000
VERSION     DACCXO010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.209"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(23..970)
                     /locus_tag="IVY70_002837"
     CDS             complement(23..970)
                     /locus_tag="IVY70_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP7959916.1"
                     /translation="MLNYLITGGAGFIGSTLANYLGKENNVVVIDDLSMGKKENLDES
                     KHITFIEGDVADKNLMEKVMKEYHFDYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(1003..>2195)
                     /locus_tag="IVY70_002838"
     CDS             complement(1003..>2195)
                     /locus_tag="IVY70_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP7959917.1"
                     /translation="YMVILVITSFVHKEYGLGENLKTIMWAILQYFLIYQFAFENSDN
                     KRFFKRISWGFIYSWFVLVSMSMVLFFGKFGYEKYYNARHRIRIGFLESRLFGTFSDI
                     NNAAIASLVVIVLILFYLYNKKVTGLNKIIFLINLPLQFIYIVLSGSRSTYVVSIIIV
                     AISSFIVVLKRNGTKKDLKNYILAIVSSIGSVLILFALFNATKILFQELLPHLKEINF
                     FNFTERHKELMNNSQHINNTQTPDNTLVRKDVAENTDISNARITIWKSAWEIFKTSWL
                     IGVSPKNVVSYAQSIIPDGYIAKVGIAIHNAYLNVLTSTGILGIIPFMAFLIKSFVLN
                     VYRIIYNKCIGKDYHFVYCLVILSYALYAALNNELVYENTVGTFVFWLFLGRVNSKNN
                     SSYF"
BASE COUNT          763 a          400 c          300 g          732 t
ORIGIN      
        1 agcaggatgc tggttttttg tttcaatcga tttctgtctt taaataacgt tccattccgt
       61 tcaatatatc aaatttgggt tgataaccta aagaacggat ctttgaaata tccgctaaag
      121 aatcacgaat atcccctgaa cgctccgctt catatttaag cgctagctct gttcctaaga
      181 tttgatcaat tgaatgaatc aattcaagta gcgtagtgct ttttcctgta ccgacattga
      241 attgctgtcc taaagctttt tcttccttag caacaagtaa aagtgcttgt ataacatctt
      301 ctatataaac gaagtctcta gattggctac catcaccaaa taaagtaaat tctgtttttt
      361 ctcctgccaa ttgttttttg tatcggtcca ctagaatcga aataactccg ctatatggag
      421 agttaggatt ttgatttgga ccataaacgt taaaaaatcg gacagcacta gtcggaacat
      481 catataaatg acagtaatcc aaaacatatt gttctgctgc aaacttatcg atggcatatg
      541 gtgttaaagg acggatgacc gattcttctt tttttggcaa cgttggttca tcgccataaa
      601 ctgcagctga tgaactaaat accaatcgtt ttaaaccaga ttgatatttt cgtaccaatt
      661 caagcaataa aagcgcactg tcaaaattta cacgatgagt ttctagagga cgttctacag
      721 aatctgccac actagctaca gcagctagat ggaaaatata atcgaaatgg tattctttca
      781 tgaccttttc catcaaattt ttgtcagcga catccccttc aataaacgtg atgtgcttcg
      841 attcatccag attctctttt ttccccatcg ataaatcatc gatcacaact acattgtttt
      901 cttttcctag ataattcgcg agcgtcgagc caatgaagcc ggcgccaccg gtaatcaagt
      961 aattcaacat tttttctcct cttttcaaga taaaatttat tactaaaaat agctagaatt
     1021 atttttgcta ttaactcttc ctaaaaatag ccaaaaaaca aacgttccta cagtgttttc
     1081 atagactaat tcattgttaa gagctgcgta tagtgcataa gataaaataa ctaaacagta
     1141 aacaaaatga taatctttac caatgcattt attatagatg attcgataaa cattcagcac
     1201 aaaactcttt attaaaaaag ccatgaaagg aattattcct agaatacctg tcgatgttaa
     1261 tacattcaag tatgcattat gaatggcaat tcctactttc gcaatatatc catctggaat
     1321 aattgactgt gcataagata ctacattttt cggagagact ccgataagcc aacttgtttt
     1381 aaatatttcc catgcacttt tccatatagt aattctagca ttagaaatat ctgtattttc
     1441 agctacgtct ttcctaacaa gagtattatc aggtgtttga gtattattaa tatgttgact
     1501 attattcatt aattctttat gacgctctgt aaaattaaaa aaatttattt cttttaaatg
     1561 aggtaataat tcttgaaaaa gtatcttagt tgcattaaac aaagcgaata aaatcagaac
     1621 agaaccaata gaagatacta tagcaaggat atagtttttt aaatcttttt ttgttccatt
     1681 tcgttttaac acaacaataa aagatgatat ggcaactatt attatactta ccacataggt
     1741 agatcgtgac ccacttaata ctatataaat aaattgtaat ggaagattaa ttaaaaaaat
     1801 gattttattt aatcctgtca cttttttatt ataaagataa aataagatta ggacaataac
     1861 aactaaagat gctatagcag cattgttaat atcagaaaac gtcccaaaca gtcttgattc
     1921 taaaaaacca attctgatcc tatgtctagc attataatat ttctcatagc cgaatttacc
     1981 aaagaaaaga accattgaca ttgatactaa aacaaaccaa ctataaataa atccccaact
     2041 tatcctttta aaaaatcgtt tattatcgct attctcaaaa gcaaactgat aaatcaaaaa
     2101 atactgtaag attgcccaca ttatagtctt taagttttct cctagaccat attctttatg
     2161 aacaaaactc gttattacta aaatcaccat atatg
//
LOCUS       DACCXO010000202         2189 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.210, whole genome shotgun sequence.
ACCESSION   DACCXO010000202 DACCXO010000000
VERSION     DACCXO010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.210"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(85..1614)
                     /gene="ahpF"
                     /locus_tag="IVY70_002839"
     CDS             complement(85..1614)
                     /gene="ahpF"
                     /locus_tag="IVY70_002839"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP7959918.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1624..2187)
                     /gene="ahpC"
                     /locus_tag="IVY70_002840"
     CDS             complement(1624..2187)
                     /gene="ahpC"
                     /locus_tag="IVY70_002840"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP7959919.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
BASE COUNT          638 a          471 c          375 g          705 t
ORIGIN      
        1 tattcaaaaa aacaaaaagg attgtgacaa aattcttgtc accatccttt tttccgctct
       61 ctgacttttt acaatcttca tagctcaagt gcgcatcaga taatcgaatg ctcctaaagc
      121 tgcagtagct cctgaaccca ttgaaacaat gatttgtttg tatggactat ctgtacaatc
      181 accagctgcg aaaacgccgg ggatattcgt tgcgccattt ttctcggtca ctatttcatt
      241 tcttggattc atttcgatcg ttccatctag ccaatccgta ttagggagta agccgactag
      301 aatgaatact cctgaaacag ctagtgtatg catctccatc gtacggcgat cgatatagga
      361 gagagattct acctgattcg taccgctgat ttctttcgtc gctgcatgtt tgataacagt
      421 gacattcgca agagagacta gtttatcctg taagatttga tctgctttta attctggtaa
      481 gaattctaga acatatacat gttttgctaa cccagccaaa tcgattgctg cttcaacacc
      541 agaattacca ccaccgataa cagcaatctc ttttccttta aataaagggc cgtcacagtg
      601 tgggcaataa gcaattcctt tatttttaaa ctctttttcg cctggtacat tgatagaacg
      661 ccagtgcgca cctgtcgcga tgatggcggt ttttgcttta agtgatatac cgttagctaa
      721 gccaatatta ataaaatgtt gctctttatt taaggaaacg gcctgttgat tcatcataat
      781 gtcgataggg taactacgca catgactttc tacttgctgc atcaattttg gaccttctgt
      841 gtaagaggta ccaatcatat tttcaatccc tagtgtttca acgacttgtc cgccgaaaga
      901 atcagcgaca agtcctgtac ggattccttt acgtgctgca tagatagctg cactactagc
      961 tgctggtcca ccgccaatca ctaatacatc ataaggttct ttttcaaaca actcttcttc
     1021 aatcaaaggg ccagtgattt tttcaacaat ttgttcgatc gtcatgcgtc cgctactaaa
     1081 ttcttctcca tttaaaaaca cagttggcac tgccatgatt tttttatcat cgatttcttt
     1141 ttggaacatc ccaccttcaa tcatcgtatg agtaatattt ggatttaata cagctaatat
     1201 attcaatgcc tgtacaacgt cggggcaatt atgacaagtc aagctaacat aactgtcaaa
     1261 atgcaatgtt tgtgttattt ttttgatttt aagactcaaa gattcactga ttttcggtgc
     1321 ccgtccacta atttgtaaca tagccagtaa aaaagaggaa aattcatgac cgagggggat
     1381 accagcaaaa acgattccac tttctttgcc aactggattg atagaaaaac taggtgtgcg
     1441 catcgaatct cttttttccc aagagatttt aggagacatt tctgctattt ccttcgtaaa
     1501 ttcagctagt ttttgactat tttcactatt atccaaacta acagaaaaaa caataggaga
     1561 ttcaatcagt gctaaatatt gttcgagatt ttgtttagtt tctttgtcta acatatccac
     1621 cacctaaatc tttccaacaa gatcaaaact tggtttcaat gtttcgccac tttctttcca
     1681 ttttgccgga caaacttcac ctgggtgggt acgtacatat tgagcagcgc gaatcttatc
     1741 aatcagtgca ctagcgtcac gaccgattcc atctgcattg atttccatag cttgaacgat
     1801 tccgtctgga tcaataataa atgttcctcg ctgagctaat ccttgctctt catccaaaac
     1861 atcaaaaata cgagaaatct gatgagacgg atcaccgatc atcacgtatt cgattttacc
     1921 gatagcatct gatgtatcat gccatgcttt gtgagtaaag tgggtatcgg tagagcaaga
     1981 aaagacttca acacctaaag atttcaatgt actgtattgt tcttgtaagt cttctaattc
     2041 agtaggacaa acaaaagtaa aatcagctgg atagaagcaa agaatactcc agttccccat
     2101 aagatctttt tcactgactt ctaaaaattc tccttgatga taagcatgtg cattaaattc
     2161 ttcgattttt ttcccaatca atgacatag
//
LOCUS       DACCXO010000203         2124 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.211, whole genome shotgun sequence.
ACCESSION   DACCXO010000203 DACCXO010000000
VERSION     DACCXO010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.211"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            230..1189
                     /locus_tag="IVY70_002841"
     CDS             230..1189
                     /locus_tag="IVY70_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7959920.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            complement(1176..1781)
                     /locus_tag="IVY70_002842"
     CDS             complement(1176..1781)
                     /locus_tag="IVY70_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959921.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNS
                     LDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKVSFVKLS"
     gene            complement(2021..>2124)
                     /locus_tag="IVY70_002843"
     CDS             complement(2021..>2124)
                     /locus_tag="IVY70_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7959922.1"
                     /translation="WTCPICHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          704 a          378 c          340 g          702 t
ORIGIN      
        1 tttatgttat ctttagctaa aacatcaaat acatgtcctt gaaattgtat cgaggcagct
       61 ggatttagtt taaattgagg aaactttacc acagctgatt caaaacactt gtcacatttt
      121 tttatttttt taccttcgcc gattgtaaaa ttaagctaga caactaaaaa agtcatttgt
      181 gctacactca aaatagttcc gtcgaaagaa ttataaggag tgagtacaaa tgacctatac
      241 ccatcttaca ccaactgaac tcgtaatgat agaagcttat ttcaatcaat cacactctgt
      301 ttcaaaagta gcccatctgg tacaacgttc tcgacaaacc atttataatg tctatcgttt
      361 tcttaaatct ggtggaagcg caataagcta ctatgaacaa tataagaaaa acaaacgtaa
      421 ctgcggaaga cgtccgattg tgttaccaga tgggcaacaa gaatacattc aaaaaaaggt
      481 ggctcaaggg tggactccag atgtccttat tggtcgagca gagtttccta ttgcttgttc
      541 ggtacgtaca ctatatcgga tgtttaaaga taaaaccttt gaccctcacg atttaccaat
      601 gaaagggaaa agaaaaccaa acggacataa agaaaaaaga ggaaaacaag cttttcgccg
      661 ttcgattcag gatagaacaa ctgattatat acagttcaat catgagtttg gtcacctaga
      721 aggtgacacc attgtcggtg gaaaacataa aagtgcggtg atcacgttag ttgagcgatt
      781 atctaaagtg atcatcacct tgaaacccgc tggtaggcaa gctatagata ttgagaatag
      841 actgaatcaa tggtttcaat ctgtaccgaa acacttgttt aaatccatca catttgattg
      901 tggaaaagag ttctcgaatt ggaaaaagat tagtaatacc aatgatattt cgatttattt
      961 cgctgatcca ggaacacctt ctcaacgagg actaaatgaa cattcgaatg gcttattacg
     1021 taaagatggc ttatataaag ctatggattt taataaggta tccgaaacct ttatccaatc
     1081 ggtggcatca aaacgaaatc atattcctag aaaatcatta cactatcgca caccattgga
     1141 agtatttttg agttacgtag gtaaagatga tttgtctagc ttaatttgac aaacgaaact
     1201 ttgtaaaaat attcgattga atattctatt tggcacgact cacttggcag tatatgtatg
     1261 tcaatatttt tcatctgttt cataggtatt tcaaaatcta aactattaaa aacatataat
     1321 tccctgtaat tatttacatc taactctact aaatttatct ttggaacaat agatcctcca
     1381 cccattccac cataataaaa aaaatcttgt tctctatcaa aatagcacat attaagctca
     1441 gtttgtttat catcctcaat aattttataa atcaaaccta tataatcatg aatgctggca
     1501 aaaatttttt ttaagtcaag ttctaaatct tcttcgaaaa cacatttaat ttctcctact
     1561 gataattgat actctatatt acatgactta attagttttt tagatccccc ctccagatag
     1621 gcatatgttt caataattat gttcttttta ttgcttggca aatttccgtt atttattgca
     1681 taaattaaaa atgactgtct atttagccca taatctaata caacttcttc atactcatat
     1741 ttctttaaat atttaatatg aaaatatgct gaattaatca aaactcgagt gttactgttg
     1801 ttatttattg ttgttctatt gtgtataaaa ttgtataggt tgtcatattt tcttggttta
     1861 gtgaactact caccacttag ctaaacctaa cggtttctta cgtttgaagt gggagcttct
     1921 tgggaagaga gcatacttga tagcgtatta ctttactaac agaggtgcct cttatttacc
     1981 aagctatccc cgtagttcct acggttcttg tttatttgat ctaagccaat cccatagagt
     2041 gtagccttag accttcggtc aggatattga tactagcgtt gatatctcgg tcatgatgag
     2101 tatgacaaat agggcaagtc catt
//
LOCUS       DACCXO010000204         2115 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.212, whole genome shotgun sequence.
ACCESSION   DACCXO010000204 DACCXO010000000
VERSION     DACCXO010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.212"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>2115
                     /gene="acm"
                     /locus_tag="IVY70_002844"
     CDS             <1..>2115
                     /gene="acm"
                     /locus_tag="IVY70_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP7959923.1"
                     /translation="KKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVDP
                     TNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGT
                     LTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVN
                     VTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLD
                     PSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKI
                     MVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGEL
                     KIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKE
                     KEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSI
                     YFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGND
                     YVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQH
                     KEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNIS
                     IPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNK
                     SKSILPKTSSKKSSFAVIIGLL"
BASE COUNT          830 a          343 c          410 g          532 t
ORIGIN      
        1 tgaaaaagtg tagaaatatt attttatcaa tgttatttat tattactaat ataacatcac
       61 tgattcctgt tcatgtctat gcggatgcag gcagagatat cagcagtaat gtcacttcgt
      121 tgaccgtgga cccaacgaat attacagatg gaggaaacat aaaagtaaaa ttctcgtttg
      181 acgaaaaaaa acaaaatatc caaccaggtg attatctttg gatcaattgg ccaagtgaag
      241 gaaatattcg cggtgaagga ttccaaaaag aaatcccatt aatgattgaa aataaaaatg
      301 ttggaacttt aacggtcaga aaagattctg cgcaggttgt gtttaatgaa aatattaaaa
      361 acctagactc agtagaaggt tgggggcaat ttgagataca ggccagaaac gtaaccgata
      421 ccggtgaaga aaatacggga ccattcaccg tgacgagcgg tgataaaaca gctactgtaa
      481 atgtgacaaa gccagcttct ggttcttctt ctagcgtttt ttattataaa acaggagaca
      541 tgctacctga agatactaag cacattcgat ggtttttgaa tattaacaat aacggcactt
      601 atgttgaaca accagttaaa atcagtgatg aaatccaaag tggacaaaga ttagatccaa
      661 gtacgtttga aattaatcaa atacacttag gtgaacagaa ggtttataga ggagaagagg
      721 gaatccaaca atttttacaa gatttcccca gcgctacatt taatttttca gtaactgata
      781 attacattga aataactata ccaaaaaact ttgtcaatct taggaaaata atggtgagtt
      841 ataagacgat catagaaaac ccagaacaaa taaattttga gaatcattcg gaagcttggt
      901 ttaaagaatt taataaacct gctgttgatg gagaaagttt caatcataca gttaaaaata
      961 tttcagcttc tggtggagtc aacggtacag ttagaggcga attgaagatt ttcaaatata
     1021 tcaatgacac agagattggt atcccaaatg tgactttcga attaagacga gcagatgaac
     1081 agcctattca aggacagtct agtatcctgt tgacttcgaa tgaacaggga gaagctagta
     1141 tcaaaggcct gcaagtagga gactatgttg taaaagaaaa agaagcaccc aattggattg
     1201 attttgatcc actaagttca aatgaactca aattctccat aaacgaaaat gatacagagg
     1261 gagtcagttt acctatttat aacaagaaaa aggttacgaa catcacagca actaaaattt
     1321 ggaacggtgg aaccactcca agaccgagta tttattttaa attattcaga gcaagtacaa
     1381 acaattggga accagttcct gatgcagaaa caaaacgatt agacaatgga ataacttcag
     1441 tgacttggga agatatccaa cagtatgatg atagtggaaa cgaatatacg tttaaagtgc
     1501 aagaagtaga tcaaaacgga aatgattatg tcccttcagg ttatcaaaaa attgaaaacg
     1561 ggctagtcgt tacaaatgag aataaagaag tcatttcagt ctcaggacaa aaaacatggg
     1621 aagacaatga gaaccaagac ggcaaacgac cgacgacgat cacagtaaat ctattagccg
     1681 acggtcaacc gatccaacac aaagaagtaa gtgaaaaaga tgattggcgc tatcgtttca
     1741 caaatctgcc aaaatacaga gacgggcaag aaatcatcta tactgtaacg gaagacaacg
     1801 tgcccgaata cagtacgacg attgacgggt ataacatcaa aaacagttat acaccggaaa
     1861 aaacaaatat atctatacca ggtagccata taacaccgtg gaaaaatttt ccagagaaga
     1921 cagagagaga gaaaattaga aatctattct ctcaaaaatt gcaaatattt gctcatacag
     1981 gagaatccag cgaacaaaga agcaattata ttagtaagag agctgtacct aaacagataa
     2041 caaataaatc aaaaagcatt cttcctaaaa caagtagtaa aaaatctagt tttgctgtaa
     2101 tcattggttt attga
//
LOCUS       DACCXO010000205         2077 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.213, whole genome shotgun sequence.
ACCESSION   DACCXO010000205 DACCXO010000000
VERSION     DACCXO010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.213"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            88..2064
                     /locus_tag="IVY70_002845"
     CDS             88..2064
                     /locus_tag="IVY70_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP7959924.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
BASE COUNT          811 a          277 c          361 g          628 t
ORIGIN      
        1 gtgatataaa tattgcaggc ttttttattg atttgaaata atcaagaata ttagaaactt
       61 ctacattaat caagaacttt cttctgtatg aaaatagaga taataattgt acacttaagc
      121 tgtattcagg aggtgaagat aatgggcatc gaatttagcg aacggcaaat gaaaatacta
      181 tactttttga ttgaaaatga gcacacaact tacaaagaat tatcagaaca attatatgta
      241 tctacgaaaa caattagcaa agatatttta attttgaaag aaataatcca aaaaatgcca
      301 gatgtacaaa tgttggttca acctaaaaaa ggcgtttcgc ttattggaaa aaaagaaaca
      361 cttcgaaatt tattatccag tcataaaatc aatatcgcag actctccgga ggaccgtcta
      421 acttatgtgt ttgctaaatt attgaatact aatgattctc taaagattca aaatcttgct
      481 gacgaattat acgtgagcag gtcgacggta gaaagcacaa tgaaagatgt tagaaaaaga
      541 ctaaacaagc atgggatgtc cttagtttca gatcgaaatg gcgtgcgcat caaagctgat
      601 gaaaaaagta aaagaaggtt gatgtcagaa gttgtgaact attattgggg tggctttaca
      661 gcatctaaca gtaaaacaga tctcttagac ttgaatatca atttcattgg tgaagcgaat
      721 aatttcttaa ataaggacat attgaaaaaa gtagccgaat tgcttaatcg atacatgaat
      781 aagacacaat tgaaatttac agattatgaa tttcaaactt tagctatcca ccttgctatt
      841 gcgatagaaa ggattgaaac aagcaatttc ttatcacgta gtaataattc tgaagaagaa
      901 atctcaaaga atacacttat tttaatcaaa atgattgaaa aagcattttc aattattgtg
      961 ccaatagatg aacaagctta tgttgacatt cacattactt cgattgaaaa aaatgctatg
     1021 aatcacgact tagatcaaac gaagctttcc tatttagata tgggtgttga gctacaagag
     1081 ttagtaagaa ttaaattaaa tgaattttat cctgatgaag aattgatcaa taatctagta
     1141 ttacatctca atgcagcagt taaaagactc aaattgggag taaatatcta taacccttat
     1201 acggacaaaa tcaaatattc ttttaaaagg tcatttatga taagtgtaga tttattagag
     1261 gaaatcgaag agagattctg tatacatttt aatgaagatg aaatcgctta cgtaacactc
     1321 catattcaat cacttttgga tcgttacaaa cctgacaaaa cgaaagtgat attagtttgt
     1381 agtagcggat atggaacatc aaaattatta gagcaaagaa taaataacgg ttttgcagat
     1441 atggttgaga tagaagatgt actaagcatc aatgaactac aagatagaaa agtcactgat
     1501 gaacttgtta taagcacgat tccgattgaa aatacaaatt ttcctgttat tgttgttagt
     1561 cctttgatgt taggggcaga tgtcagaaaa gtaaaacagt atctacagtt aaatgaacaa
     1621 acagaagatg cttttctaaa tttattagat aaacgatttt gcttttttac ccaccaaata
     1681 aaggataatc aagtgaacgt tttaaatata attaccaaaa aattggtaaa agaaaattac
     1741 gcgaaagaag ggatactaga gggagcaatc aaaagagaaa aactatcttc aacagcgatg
     1801 aatgattttg ctatgccgca tatcgaagta gattttataa aaaagccagt aatctgtgtc
     1861 tacgttaatc cagaaggaat tgattgggat gggacacttg ttcattctgt tttctttttt
     1921 gcacttaatg aaaaagtaaa acctgaattg aataaaattt atgaaacatt caatgaaatc
     1981 gcttcgaatc atgagttatt agcaaattta aaaacgagtt cgtcgtttga aaaattagtt
     2041 tatatattga aagaaggctt ataaatgatc agtgaaa
//
LOCUS       DACCXO010000206         2039 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.214, whole genome shotgun sequence.
ACCESSION   DACCXO010000206 DACCXO010000000
VERSION     DACCXO010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.214"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..425)
                     /locus_tag="IVY70_002846"
     CDS             complement(<1..425)
                     /locus_tag="IVY70_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP7959925.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(534..1565)
                     /locus_tag="IVY70_002847"
     CDS             complement(534..1565)
                     /locus_tag="IVY70_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7959926.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          627 a          331 c          351 g          730 t
ORIGIN      
        1 ggaatctgtt ttgattttgt tccatttgaa atttctctca ctcgcttttt tgtctcatca
       61 tcaaataaaa tgaaagggtc ttctaaacga tcgataaata cttccattgt tgttgttggt
      121 agatgataag aagtcatgac aggcactgca ccaaggtaag acacagaaac agctagaagg
      181 tatgtgtcaa aagcggaact cttataaata ataactttgt ctccggattt cactccaaga
      241 tgagccaatt gataagcccg tttcaacaca ctttcgcagc attttttgta agttgtttca
      301 agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga taatcggggt ctcggaaaag
      361 ttagctgcag cttcttgaaa attcgtaaat aagttcaaag gggaatacgt tatggtctcc
      421 atcattcaca ctccattatc ttttgttaag taatatacaa attcaagata atcgtaacat
      481 aatcctttat ataataacat agtttattgt gaaaaaaaga tcatacattt gttttatatg
      541 gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa aatcaaccat cttttcccag
      601 atatcttgaa aagaatggga tgagaggtat cgattaccac ataaataatt taccgcgtca
      661 gcatcatcta aatatagttc tgcataataa cgatatgttt ttctgcatag ttctggcgat
      721 acgtttttat atactgtaat tttgctcaaa atttggcgaa tagtccgagg agaaatttgc
      781 tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg tattttgtgc tgttaactta
      841 aaatagttct ccagaaatgg catcgcttct tttggtattg gtactttatt cagtctgttt
      901 tcttttctat tcagattaag aatctgcttg gtcaaagata catctttacg tttaagttct
      961 actacttcat ggatctttaa tccgcaatac agaagaatgg cagtaattgc aaggttcctc
     1021 ggtctattct tttcttcaaa gctagcttct ctgcttgttt gaaagccttc aatcgggata
     1081 aacaaatgct caaaatcttt gaaaagttct gcatcgatat atcgcggagc tgctttggtt
     1141 gtttccggta aacttgtacc caaaagagaa atctcttctt caatcagtcc tttttgatac
     1201 aaaaatcgta aaaaatgatt taatgaatat tgcttccttg aaatcccgta atagctattt
     1261 tcaaacaatt gctccgtttc ttggttcgct gtagtttgga acgaacgagt atgtcgatag
     1321 attttagcta agaaattctg ataatcttgt ttttctaaat ggataatcag ctgatcatcc
     1381 acttcttcag aaaatacttt tctcaaataa tcagtaaaca gatgaaaatc ttttgcgtat
     1441 tcatagcgac tgcctaatga aagggaagaa cgattttctt ctaaaaactg ttgaaagacg
     1501 gaagggagca agtgtgtacg ttgctttaac ttttcttgca gttgttttct agttttgctg
     1561 tccattacaa attccctcca atagtgttca ttttctatat tttaccgcat ttcatcaaat
     1621 gaagatagac agtttgtctt gatcttagga agcaaatttt taactaagta ctagctaaaa
     1681 gatatgaagt aagaagatac atttttagtt attttattag tgcagtcatt cttttgatct
     1741 tttaattgat ttatcctctt tttagctatg tggttacttc atgagcaatg aaatggattt
     1801 ggttcgttct aatggcagga ggctctttct tttatttccg aaaaattaga aaacattttt
     1861 tcagaatcga ttggctctcc atttattcaa taatggtttg tctaacgata ttgttgggat
     1921 aattcttaaa gaagtattag aaatagttgg tacttcatta ctttacatga agatttattc
     1981 tatggtagga gcaattatat tgagaattaa tcctttttta tataagttgt ttgcttatt
//
LOCUS       DACCXO010000207         2014 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.215, whole genome shotgun sequence.
ACCESSION   DACCXO010000207 DACCXO010000000
VERSION     DACCXO010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.215"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..581)
                     /locus_tag="IVY70_002848"
     CDS             complement(<1..581)
                     /locus_tag="IVY70_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959927.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITASQAWQNAKTLAKPVIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLG"
     gene            complement(591..1328)
                     /locus_tag="IVY70_002849"
     CDS             complement(591..1328)
                     /locus_tag="IVY70_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7959928.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            complement(1379..>2014)
                     /locus_tag="IVY70_002850"
     CDS             complement(1379..>2014)
                     /locus_tag="IVY70_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7959929.1"
                     /translation="SGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
BASE COUNT          611 a          410 c          303 g          690 t
ORIGIN      
        1 cctagccttt tttcatgacg taatgtgtta ttatctttta gaaattcccc atgaaatcga
       61 tctagaatag atccctcttt tccaccaaag aattctaaaa aattcgcctt ttctatcttc
      121 acattagcaa gcgtatctac taatgacgag aaagaaaact gtgaaggaat aactggtttc
      181 gctaaagttt ttgcgttttg ccatgcctga ctagcagtga tcttttctgt tccactgtca
      241 tatttattca acaccgattt tcttatatca ttgaaaatag gttcagcttt tacttctatc
      301 gtattgccta ttacagaagt ctttgcataa taaatccgta gacgctgttt tgctcgattt
      361 tcatctacat aacactgaat aatacgtcct tctacaatca aatctgcatt agttccgctt
      421 attggataag taccctgaaa tatctcggct ccgtttaatt tattgctaac agtagctgtt
      481 aaccagtctg acaaagcgcc taaaccttgc gtatcatata aatgttcagc taaattattc
      541 gcgtcgtttt tatcgtaaat agttattaaa ttatcgatca tctatttcac ctaccttaac
      601 ccattacgat aaatttgtat tttgctcaaa ccagtacaat taaaataatt aatatccact
      661 tgcaatgtcg gatattgcat ggtcttcatt ttgttggacc gatctaaaat atctccgtcc
      721 gattgctctt cgtagcaaag catcaaatca ctatcaatga ctacgtcagt tcctactact
      781 aagccttcaa aactaaacac ataatcattt aagatgaact ggcatgaagt agctgaagga
      841 gtgatgataa tctttggaaa actttcttct aaactattat tcagcaagtt aaatgactgt
      901 ggtttatcta cggttatagg tacatcttct tgaactcttg cgaatggttt cgcagtaata
      961 tttacatcga actctcccca ttcaacaata tcgttttctg catccccaat atcgatagtc
     1021 tggataacat aatagacgtt gggatcatca gagaattcta atttctttgc ataatttaac
     1081 caatgacgca tgatataaaa cgattgcttg aacgcttgat ggtcttccac atcctctaaa
     1141 tagttatagt gcaatgtaaa cgacatgtct tcaaacgagt aatcttgtac taagccacct
     1201 aaccttccta aaacagaagt ttcaactctc tgtctttttg gagaaggtat ggttggtctt
     1261 tcagctaaag ccaatttatg caaataatca ggaaatccat cgattataga atgtatacaa
     1321 tcagtcattt tttcacatcc tttttaatac taaaaaaaca ggagaaatac tctcctgttt
     1381 aacgccatgc cgaagcatta tcattttgaa cttttgtaat gctatcaatg atttgttgag
     1441 ttgtttgctt catagtaacc tcatctgcgt taccatcaat tgtgaaattg aattcgtagt
     1501 tattcacagg ttgaatcgtt tgtgccctag atgaaactga tgtgctactc aagatacgat
     1561 caccaatttc ttgcagcaca gatcttttca aaggtaaaac tgcttcaggt cctgcttcgc
     1621 cgacaccgat aatattcgga gaattaaaca cactaccttt cgcataccaa tcaacaccca
     1681 acgttgggat tttccccttc aatggattga attctccgct caatttaaaa tgtggtaacg
     1741 gaatatgtgg tatagaaata ttcaaattat caaagatact actgatttta tctctgatcc
     1801 aatcaattgg agcgctaaca gtctttttga taccttccca aatgttagca attgtacttt
     1861 taacattatt gaatatgtcg gaaacaatac ctgttagatt ggaccaaccg cttgaaattg
     1921 cattttttcc atcgtttact ttagagctaa tagtgcttgt aattccattc caaagattca
     1981 aagcagtgtt tttgataccg ttccaaattc cgct
//
LOCUS       DACCXO010000208         2003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.216, whole genome shotgun sequence.
ACCESSION   DACCXO010000208 DACCXO010000000
VERSION     DACCXO010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.216"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..378
                     /locus_tag="IVY70_002851"
     CDS             <1..378
                     /locus_tag="IVY70_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP7959930.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="IVY70_002852"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="IVY70_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP7959931.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="IVY70_002853"
     CDS             1859..>2003
                     /locus_tag="IVY70_002853"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP7959932.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DACCXO010000209         1952 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.217, whole genome shotgun sequence.
ACCESSION   DACCXO010000209 DACCXO010000000
VERSION     DACCXO010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.217"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            271..837
                     /gene="amaP"
                     /locus_tag="IVY70_002854"
     CDS             271..837
                     /gene="amaP"
                     /locus_tag="IVY70_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP7959933.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            850..1038
                     /locus_tag="IVY70_002855"
     CDS             850..1038
                     /locus_tag="IVY70_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP7959934.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1051..1608
                     /locus_tag="IVY70_002856"
     CDS             1051..1608
                     /locus_tag="IVY70_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7959935.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1640..1882
                     /locus_tag="IVY70_002857"
     CDS             1640..1882
                     /locus_tag="IVY70_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7959936.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          713 a          304 c          404 g          531 t
ORIGIN      
        1 taaatagaat cttgtaataa taactgagca taatctttgc aatttttaat cttgaataat
       61 atcctttaag tatagccagc aatcaaaagt aaatagagaa taagtaagca attgttattt
      121 cagtatcaga ctggcaataa aacgccgtta tgatccagca atgtttggta gcgctttatt
      181 tactgatgct atgctaactg ttcgctatct atactgtgat tgtcggtaga gttatttaca
      241 gggtaaaaat aagtttatag gaggtggaaa atgaataagg ggttaaaaat aatcggtgtt
      301 atcatgagtt tactgctact ttcagtattt ctagcgattg cgttgatcaa tgcgccgtat
      361 attatgcctg aacaattgga acgatttcgc ttctttactt taacgaatta ctacatgcaa
      421 caatatattt tctgggcagc tgtagtattt gccgttcttg ctattatcat cttgctagtc
      481 gttcttttct atccaaagtc aagaggaact ttcgttatga aacgggaaga tggcaaacta
      541 acaatcgaca aaaaagcgat cgaagggttg gtacgttccc atttacacga agaagagttc
      601 attcattcac ctaaagtacg gatacgttca acgaaaaatc gaatccatat ccatgtcaac
      661 ggagacttga aacgaacttc ttcattagta gggaaaacag gtgtattgat gcaagatatc
      721 gaagaagaag taactaaagt actcggaaca aaagaaacgg tcaaagtggc agtcacatac
      781 agcggttatg aagaacaaga agatacaaga gactacaagc actctcgagt ggaataggag
      841 ggccaaataa tgaaagaact attttcagca tacaagctcc ctattatctt tgggggtctt
      901 ggactactgc tagctgtttt atggttaacg attggatttt tcaagacatt gttgatcata
      961 atcatgacag caattggcgc aggaatcggt ttttatttga aacagtcagg aatagttgat
     1021 caatttttta aatcatagga ggaaacaaag atggaaaaca aaaacatgaa tacaacaaat
     1081 ggatcagttt tggcaaatga aaaaggagaa aaccgcatga tgaatggtga attgacttat
     1141 gaagacaaag tcatccaaaa aatcatcggt tatgcattga aagatgtaga tggtctattg
     1201 acattagatg gcggattttt ctcaaatatg gctgaaaaat tagtcaacac agataacgta
     1261 acagcaggta tcaatacaga agtaggtaag aaacaagtag ctgtggatct agatgtgatc
     1321 gtagaatacg gtaaagacat cgaagatatt tacaaccaaa tcaaagaatt gatcagtact
     1381 gaagtaaaca atatgacaca tttagatgtt atcgaagtga atgtgaatgt tgcagacatc
     1441 aaaacacaag aagaatacca aaaagatcaa gaaacagtac aagacaaagt aacagaagca
     1501 gcaaaatcca caggacaatt tgcttcacgc caaacagata aagcaaaatc tgctgtaggc
     1561 aaaggtgcac aaaaagtcaa agaaaataac gaaccacgtg tacaataaga agtaagaagt
     1621 cagttaggag gaatttaaaa tgggatttat ttgggcatta atcgtcggcg gtatcattgg
     1681 agcaattgca ggagcaatca cgaataaagg atcatcaatg gggatcatcg caaacatcat
     1741 tgctggttta gtaggttcag caattggaca agctttacta ggaacttggg gaccaacaat
     1801 tgcaggaatg gctatcattc catcattgat cggtgcaatc attttagtat tagtcgtttc
     1861 attctttgca cgtaaattat aagcaaattg aagaaaaact tatgttgaaa tgaagaacaa
     1921 atttgagaac cgtagcgaca ctcgctgcgg tt
//
LOCUS       DACCXO010000210         1895 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.218, whole genome shotgun sequence.
ACCESSION   DACCXO010000210 DACCXO010000000
VERSION     DACCXO010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.218"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(14..271)
                     /locus_tag="IVY70_002858"
     CDS             complement(14..271)
                     /locus_tag="IVY70_002858"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959937.1"
                     /translation="MSTPSRGIKNTPVLTGVWVTDRLDFNWCQKLMSRKGDEILAIYG
                     YSRISTSNQDYKTQIQTLENAGAEIIFLENHWIIEHLWKYF"
     gene            complement(339..794)
                     /gene="tnpA"
                     /locus_tag="IVY70_002859"
     CDS             complement(339..794)
                     /gene="tnpA"
                     /locus_tag="IVY70_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP7959938.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1037..1895
                     /locus_tag="IVY70_002860"
                     /pseudo
     CDS             1037..1895
                     /locus_tag="IVY70_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          602 a          368 c          355 g          570 t
ORIGIN      
        1 atacttacgt aactcaaaaa tacttccaaa ggtgttcgat aatccagtga ttttctagga
       61 agattatttc tgcgccagca ttttctaatg tttgaatttg tgttttgtaa tcttggttgc
      121 ttgtgctaat tcttgaatag ccgtaaattg ccaaaatttc atcacccttt ctggacataa
      181 gtttctgaca ccagttaaaa tcaagtctat cagtaaccca tacacccgtg aggacgggtg
      241 tatttttaat ccccctagaa ggggtcgaca ctcgctgccc cttaaaaggg gatttttaaa
      301 agaccgccaa ctgctcatct tcactcactt acttctcttt attttttaaa tggttctacg
      361 tactctcttt tacttataat atatcgaatg cgatcatcag cttcttgttc acggatgtat
      421 ttttcatttg tcttttgatt caatccgact gtactcacat agtatctctt cgaccaaaag
      481 gatttgtttc cgtatttata tttgagattt gcgtgctgct cgtggattag taccgcacta
      541 cgacctttca gataccccat aaaactggat atacatattt ttggtggtac tcgaacaagc
      601 atgtgaatat gatctagcat tgcatgtgct tctatgattt ccacatcttt catttcgcac
      661 aacttcctaa gtatttttcc aagatctttt cgtaattgcc catagattac ttttcgttga
      721 tattttggtg taaaaataat atgatactta caattccaac gagtatatgc taaactttta
      781 tcgtcatttg acatgaaaaa actcctttcg atctgatgag cggttggtag ccactcacta
      841 gtatatcgaa aggagttttt atcgctaaag atacttcttc cacgcgtaga aacgcgcggt
      901 tttcttgttt cgcacgcttt acgcacaaaa ctgactgaag tcattaagaa aataaaaggg
      961 tctaaaactt ttaagttttg taccgacccc caaaagttag accaaaaaat ctaacttttg
     1021 gaggtcggta ttttttatgg ctaagtatac ttttgaactt aaattaaaga tcgttcatga
     1081 ttacttggat ggtaaaggcg gaagtgatta tctggcaaag aaatattcca ttaaggctcc
     1141 atcacaagtg aagcgctgga tcaatgccta tcaagaattt ggtgaagagg gtctagtaag
     1201 aaagcgacaa aagaaaaaat attctgttca atttaagctg gatgcgatag agttatatct
     1261 aacaagtgag ctgtcctatc gtgaagttgc caacacactt aacatgaaca accctaattt
     1321 gatcgctagt tggatgcgtc aattccgtga gggtgggatc gatggactct caaaaacgaa
     1381 aggatgtcca cctatcttgt ctaagaaaaa tgaacccaaa aataagaaaa aatctacaac
     1441 taaagcaacg tctaaagaac gtgaacgcat cgaggagttg gagaaacgag tccgttccct
     1501 tcaaattgaa aatgcctttt taaaagaatt gaggaagctg cgaaaacagg aagcccaaca
     1561 acgacgaatg aagcaatcgc acgaatcatc gcaagcctcc gaggaccgtt taaattagtt
     1621 gagcttctag aaactttaaa gttccctaag gcaatcttta tgtactggca aaaacgattg
     1681 gaccgtaaaa atccagatca agagattgaa gcggaaatgc ttaaagttcg ggagaagcac
     1741 aaggattatg gctgtttacg gatgaccaat gaactaagaa accgcggttt ctctatcaat
     1801 aaaaagaaag ttcagcgcct gatcaagaag ctaggaataa aagtaacgac atacacacgt
     1861 aaatcacggc gctacaattc ttatcgagga caagt
//
LOCUS       DACCXO010000211         1860 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.219, whole genome shotgun sequence.
ACCESSION   DACCXO010000211 DACCXO010000000
VERSION     DACCXO010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.219"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            97..579
                     /locus_tag="IVY70_002861"
     CDS             97..579
                     /locus_tag="IVY70_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959939.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            776..1666
                     /gene="pstS"
                     /locus_tag="IVY70_002862"
     CDS             776..1666
                     /gene="pstS"
                     /locus_tag="IVY70_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP7959940.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          618 a          317 c          382 g          543 t
ORIGIN      
        1 tgccattatc tctaaaaaaa aagtttttcc ccctaatttt tttagataga aaattgtata
       61 gtatgatgag agaaaaagta gtttggggga acgttaatga gaagaaaagc agtaatcgat
      121 cggctttatt tatttttttg gttttttcct atcgttttgc taccttttac atgggtaaat
      181 gctgttgtac aaggtgcgga gatattaggc ctgatccaag gaagcggatt tgtggtttct
      241 aggaattttc cagctttgat tgttttctat ttagtgataa gtgtaggtct cttattacca
      301 aaatctctga tcaatcacta caattggcag ctgctttact tttcttgctt attagtattt
      361 acatctatgt tttctttgct gcctcgcttg attatcggcc ctaatggttt gagcgagagt
      421 ggattgaagg atttagctta ttcctccatg tatttcttga cgatcaaacc atggttctat
      481 ttagtaattg cctcatttat tcttgcaaca tttttttact tctggacaga aatcaggata
      541 aaaagtgaac gatctgtatc tataaaaaat aactattgat ggtggatcat cagtaaaaaa
      601 acatatgagc tgcgtaaaat gcatgtttct catatgcttt ttttattatt atgtaaagaa
      661 tgcgaaaaac gtttacatga tttttacata acttaactat atttttacag caaccatacg
      721 agggatttac agtcgactga taaactttag ttgtaaataa gaaaggagca ataaaatgaa
      781 aaaagtattg tttactctag gtatgatggg aattctttta gccggatgcg gctcaggatc
      841 aggaaataca gatggctctg ccacacaaaa taattcaagt gactcaaatg aacaagtaaa
      901 aatcgttgcg gtaggttcga cagcattaca gccgttagtc gatgcagcac aagaaagctt
      961 tgtacaagaa aatccaaatt atcaaatctc tgtacaagga ggaggaagcg gaactggttt
     1021 gagccaagtc gaagctggag cagttactat cggaaactct gatgtatttg cagaagaaag
     1081 agacggagtt gatgcttcaa aattagtgga tcataaagta gcagtggtcg ggatggcacc
     1141 aatcgtcaat aaagatacag atgtcaaaga tatcaccaaa caggaattga ttgatatctt
     1201 tacaggaaaa atcactaact ggaaggaagt tggcgggaaa gatcaaaaaa tcaacgtcgt
     1261 gaaccgtgca aacggcagcg gtacccgcgc gacattcgaa aaatggggac tggatggtgc
     1321 tacccctgtg cagtcccaag aacaagattc atcaggaaca gttcggcagc tggtaagtca
     1381 aacaccagga gcaatcagct atttagcttt ctcatatctc gatgattcca cacaagcact
     1441 aagtatcgac ggtgtagaac caaaagaaga gaatgtagca gataacagct ggggaatctg
     1501 gtcatatgaa catatgtaca cgaacggaaa accttcccct gaagtccaaa aattcttaga
     1561 ctacatgatg acagaagaaa tccaagaagg acctgtcaaa gaattgggct atttaccaat
     1621 cacaatgatg gaagtagaac gtgatcacga aggaaatatc aaataactag cagataaaca
     1681 tgccacggta gtcgttacta ttaaggaaaa atcaactatg tttccccaca tatacatttt
     1741 tcctttaatt gtgaaaaata gttcctcata ttttgccgtt ttttgagcta ataccgaggg
     1801 tcagctgatt gaacaccgtt tattcgaaaa aaagagctac gacatagaac tgtcgtaact
//
LOCUS       DACCXO010000212         1812 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.220, whole genome shotgun sequence.
ACCESSION   DACCXO010000212 DACCXO010000000
VERSION     DACCXO010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.220"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            644..976
                     /locus_tag="IVY70_002863"
     CDS             644..976
                     /locus_tag="IVY70_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP7959941.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYAGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            1595..>1812
                     /locus_tag="IVY70_002864"
     CDS             1595..>1812
                     /locus_tag="IVY70_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP7959942.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYYQKLTDNPIGFGLLPEDDEGNE"
BASE COUNT          618 a          258 c          375 g          561 t
ORIGIN      
        1 gagaacaaac gtttgcatgt tgctgttttt tagttataat aatacgcgaa gagaggtaat
       61 ttcatttttt aaaaaagcta aaaaccttga tattacttct tttcaggcaa tacgaatatt
      121 attcgcttac atttttttgt ataaatttag ttgttttaaa tttaattgtc tgctacgctt
      181 gtgatgaatt aattttcgtt ctgcactttt ggacgataaa tttggacaat tgaaaataca
      241 aaaaaaagaa aggccctgtg agactcggaa ggctggtaac cttcgtcgac gtccacaggt
      301 ggcattaaga caacctttaa tcccttaatg cgtttacctt tcagtgttac ggtattaccc
      361 gcaacggatt tagtataaca gatattcaaa agtgatacca ctttttttgg ctgtgttaag
      421 gttaaaaata cgtgatatta cgatgaatct atgcctaagc ataatgttat aaaaatgtcc
      481 gtgtggctct ttgccgtatg acgaacgctg aaattagagg gaacgcatgt ttggatgcgt
      541 tccctctttt ttgtttgtca tgaacgaaaa tcaattctct atttttgaaa aataacaaga
      601 aaaacttatt aaaaatattt aaagtaagtg ggtggattat tttatgtcat atacaggaat
      661 tttgagtctc gaagatattt gtcactacgg taaacggtgt accgctacag agaaaatcac
      721 gaagaaacta tcaactggcc aaagtaaaac cgttgtgcag tgtaagaaat atataatcca
      781 gaaagacaaa gtctcagagg aaatgattta ttacgctggt aaacaaaagc aaataatttt
      841 aaaagatcct atccctttaa aagaactata tccaacaatc aaacatgttt atgaccaaaa
      901 cggtgtactg attggtcgaa gaaaaaatgg agtcttgcgt tgtaccgcaa aagggatggg
      961 gcgtttaatc agctgaattg atcattctca taacttgtat tttatcgttt ttcgcttgct
     1021 tgaaaatatt gtcgagactt ctaaaagaac ttagaattac tagaaaaata aggagtagac
     1081 ttgatctatc aacaattcct ttcggggggt gacggggtag gtttttacga ttggtaacgt
     1141 aaaaaattgg tcaatcgagt aaatgccctt gtttggatag tggtgaaatg aatttgtgta
     1201 aataatatgc acagagtaaa caagagggaa tttagaagtt agacaagtta gaatagcgcc
     1261 tagattaatc atgagcagct actaagggat ctaggctttg acgcaaactc atggaataag
     1321 gagtaggtct tctcgtaatg gggaggacgc acaagaaatt gtgtcgtgtg ctgcagtaca
     1381 tgtagatact tgcgaggacg ttggtcattg gatcaatcga gcatttaccg atttctacta
     1441 gggtttctcg tttcggacgc cataaacgag tcgatatttt ttatgtattt ttaagtaaac
     1501 gaaaaacgat aaaatatgaa cgagtcctga ataaagtgaa aaaattgata taaaacatag
     1561 agaaaatgaa atttacgtaa tggaggaatg acaaatgaaa aattatgatc cgaatatacg
     1621 attgggcaca catactatta aggtttcttt ccaacgttgg gattacaaag gatttgtgac
     1681 gtttagaaga ggcggaaatt gtaaaggatt agatgtttta gcattagacg aggatgattt
     1741 atactatcaa aaattgactg ataatccaat tggattcggc ttgctacctg aagatgatga
     1801 aggtaatgaa tg
//
LOCUS       DACCXO010000213         1807 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.221, whole genome shotgun sequence.
ACCESSION   DACCXO010000213 DACCXO010000000
VERSION     DACCXO010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.221"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            44..1807
                     /locus_tag="IVY70_002865"
     CDS             44..1807
                     /locus_tag="IVY70_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959943.1"
                     /translation="MLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDS
                     KYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNPV"
BASE COUNT          584 a          337 c          305 g          581 t
ORIGIN      
        1 tttttattta cgtataaagt ggagcataat cattatatta tcattgttat ttttaacagg
       61 aattgcttat ttcttttatt ttattttttc tcctgcggac agcaatcaaa tgaaagaaat
      121 aaaagattcg tgggttttct atacaaaaga tgatcctgat tttaagtttg acagcaagta
      181 cactcgtacg attcctaccg tggataaaga cgaaacattc attatggaaa cgacacttga
      241 aaaaccgttg gacgaagcaa atttactaat caaaggaaac catcaatgga ttactgtata
      301 tctaggcgaa aagattttat accaacggaa agattatcag gaagaaagca atcccggact
      361 ctcattggct gtgatagatt taccagcaaa ttatatcgga aaaaaattga cccttacagt
      421 ctcatctcct tatcagaact atgctggact gccaccgaaa gtttatctag gaacaactgg
      481 accattgatt agttttattt attcacaatc agtgccgcaa gtattgacga tgataatcgc
      541 tgtttttatc gctataggga cctttttcta tacgatctac ttgatgtata aacaaaaacg
      601 tcttgaatac tcacttattc tcttaagttg ctttgctttg gcattaggat ttgaagctgt
      661 atcagaagat attttaagtg gtattctttt tgaaccattc gttcattctt ttttatctca
      721 tttatttatt atcttaacgt cagctttcat catctgctac tattggagcc gaatgtttta
      781 ctataaaaaa tggtacggaa ttttttgtac gttccaactt ttgattttta tcggcgttct
      841 gctttatgca acttttactc ctgcagaact tcctgaatta atgccgattg ctaatattgc
      901 tagtgttata agcactttag tcacttcact tgcatgcatt ggcgaagcat ataagaataa
      961 ccgtttttat gtagcttgta caccttggat cgtcttggta gccattatcc attgcttgat
     1021 ttacatccaa acggcattag gtatctatca aacaacgatc aattggtcta cgattttata
     1081 tattatcatt ctaatcgtta ttatcagcta caatctaatc gatcacatct taaataccga
     1141 caaagatcgg agacaagtag atttcttaaa aatcaaaaat gatttgttgg agcaacatta
     1201 tgagcagctt cgacagcata tccaagaagt aaatacactt cgcctggaat ttgtccaaga
     1261 gatggagaat ctgcaagatt taatgcatac ggaccaaaca aaagctaaaa actatgtgga
     1321 aaagatatta gaagaagcaa aaaatttcga aatgctgtgt tctttttgca accaccaggt
     1381 gacaaatctt atcttagcgc gttaccagaa gttagctgcc aaacggaata ttcagataac
     1441 cttccaagca gatcttcccg aagaacttcc tatcaacgat gacgatctgg ctcaactttt
     1501 gatccacgct ttagagcatt cctttagaga aactcatgcc atcgaaaatc cactgtatcg
     1561 aaaaatttat ttgtcgatac acgaacaaga ggaacatttc gtcatttgtt gcgaacatag
     1621 tgcacattac gatacaaacc tatttagtcg aggcatcaca gaagaattag atgatcagga
     1681 acgttttgac ttaatgatga tgaaagacgt agcggatcgt tacacaggaa cactaacgca
     1741 ggaaaaagat actttcattg atcggatcac tgttcaactc tctcgaaatt acccgaatcc
     1801 tgtataa
//
LOCUS       DACCXO010000214         1766 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.222, whole genome shotgun sequence.
ACCESSION   DACCXO010000214 DACCXO010000000
VERSION     DACCXO010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.222"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(145..459)
                     /locus_tag="IVY70_002866"
     CDS             complement(145..459)
                     /locus_tag="IVY70_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959944.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            745..984
                     /locus_tag="IVY70_002867"
     CDS             745..984
                     /locus_tag="IVY70_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959945.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(1346..1606)
                     /locus_tag="IVY70_002868"
     CDS             complement(1346..1606)
                     /locus_tag="IVY70_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959946.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
BASE COUNT          616 a          306 c          232 g          612 t
ORIGIN      
        1 tataaactct agagaaatta attcatccta acataaaata attgttcact caaaagtttt
       61 tctagaacaa ttaaaacagc aggcttcaca ttcgctatac atttgtaaga agtaataatt
      121 cctgtactaa aaggcataat tgctttaatt attttttgac ttctcttcaa acacaagaag
      181 tagttgtgta ataattgcag aaataaggat tccaataata gatataaaat ttgacatgga
      241 aacgtctata acattaaaag aataaagtgt tgaagaaagt aaactgattg aaataacccc
      301 tccgtaaaaa atagtctgtg aatataccca ctttttttca gaagacgtac ttcgtttggt
      361 tctaaaacct atccctgcat ttctactctt atcaaaaaac caaatattta gaagtaataa
      421 tattgctatt attaagataa aattactgta aaaatccaaa agctatcctc ctagcaacta
      481 tttaaatacc caaaaaaatt acctctagat aatatcatac ctacgttttc acttataaat
      541 tttttcataa aaaaagcgtt tagaggcata atcgttaata cagatggtaa ccaatgtatc
      601 tggtttgaac tgggatatca ccctctccat atatgtatta ctaagactaa tagatactat
      661 aaaagacttg atagttttgc attgattttg cctaatactg agcataggtg tatgctaaac
      721 gatactataa ggagtgtgta cataatgaaa agaattctgt ttatcagttt atcaaattta
      781 ttgttactta ctgcttgtgg gacttctgca tcaaagaaca taccttcaga tacgcaaatt
      841 tcgaatagta tcgattcaac aatttctaca aatgagaaaa attctccggt agaaacttcc
      901 gatataaaag tttctacctc tgcaaaaaac agggtaaaga aactgtcagc acgtctcctt
      961 tatttcaaac aaactctgaa ttagcttctt catcttcttc agcaaataat gatgatagtt
     1021 tcgcgtttac tcctaactaa atgcaacctt tacaaatgct ttacgataac aagcctgagc
     1081 taaatgatcc tgatatcagt tttgcatttt ttgaaatgat tggatcggat gatcttttta
     1141 aaactactag tttgtctatc cgaaaatagg acagttctgg cagtataggt ttttatcgag
     1201 tttctccacc aggcctcgtt acagagacag attctctagg cgaacctttt gaataaatca
     1261 aaaatcatga acaatcactg ccaactaaat aaagtgcctt caaaagcttg gtcttctcgc
     1321 ttttgaaggc actttattta aatctttatg aaactaaagt atgttcatat ttgttgctat
     1381 agttatatag atattctgtt gtttccaact cataaacgtg agtatatttt gccactactt
     1441 ttccgcattt ttctaatatt tcattgcgat cgacgtcatg atattcgtct acgcagatca
     1501 caacacaatt ttctaattta ctgattactt ctccaatcac tttttgtttc aagccaatag
     1561 gttggcaaat atattttttt cctaaaatga tagtttcgtt attcatattt catccatcct
     1621 taatgttttt tcattattta aaatgataat aacattaaaa tatgattatg aaaatcaata
     1681 acacttgtga ttttataaaa aaataaaaaa accgaaaaac agaaatgccc actgtctttc
     1741 cggctctttt tattcttgat ttctct
//
LOCUS       DACCXO010000215         1734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.223, whole genome shotgun sequence.
ACCESSION   DACCXO010000215 DACCXO010000000
VERSION     DACCXO010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.223"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            883..1095
                     /locus_tag="IVY70_002869"
     CDS             883..1095
                     /locus_tag="IVY70_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7959947.1"
                     /translation="MKNRLEELRKQRGIKQEDLASALEVSRQTIGSLENGRYNPSIIL
                     AFKIARYFQMSIEEIFIYEEESEDEK"
     gene            1085..1480
                     /locus_tag="IVY70_002870"
     CDS             1085..1480
                     /locus_tag="IVY70_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959948.1"
                     /translation="MKNKGQIWYIGYIVSLFLIVTILVTDFPKMVDIGLAILFSAVFG
                     VSHVQILHDKMIKNDADYKINVMDERNIIIKEKAGNITNMFNTILLGVATVIFICLDY
                     IIPAIIVGSIIAIQPIILIIISSVLEKKM"
     gene            1573..>1734
                     /locus_tag="IVY70_002871"
     CDS             1573..>1734
                     /locus_tag="IVY70_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP7959949.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEY"
BASE COUNT          607 a          252 c          299 g          576 t
ORIGIN      
        1 ttaataatac tgatccctaa taggtgtaaa acagttataa gaaatgagta ctctagtatt
       61 tgtgttaccc gaataattac ttgattatat tggtttccca tataattgaa ggctgtccta
      121 atttttacct actatatgat cataattgat taactcttat atatttccga aaattatctt
      181 gaagatttct attggtatgg agaatatcca tttcattgcc aataaggata taaattctca
      241 aaataacaat cgatgtctgt attaaacaag cctagaatgg taaatctgat tcgccaaaac
      301 cattggattg tatcgatttg ctttacgcga acgccaccta cccattttat aaaaataaaa
      361 tgggcaaaag atctttcttt ttgtcataca aaaagaagaa aaaaaagaga cgattttctt
      421 cttccaaaat cgtccctctt ttttctaaaa ccatggatga acgaacaaaa aatctctcgt
      481 attttgtttg ttcatccact aaaaatatgc gaacagtatg gtttcattca atcctgttct
      541 tacaagtcaa aagcatcgaa tcgtccttgg acgattcaac aaaaaaagta gtcgtttttg
      601 gatgactact aagttctaaa cattcattac ccaaaatcaa tttggtctaa catcgtttct
      661 cgactggctt gatctaatcg gctcttgaca gggctttttc ttttctgttg atagcagata
      721 agaggaaata agaataagtg tgttcatgtg tcataaaaac agtaactaaa acattaaaat
      781 caaaatttat atggaaagct tccttgacat tttgtgctcg caggagtatt ataaaagcac
      841 gaaaggaaag taaactttcc attttttaga aaggagcatg ttgtgaaaaa tcgtttggaa
      901 gaattacgaa aacaaagagg aattaaacaa gaagatttag cttctgctct tgaagtatct
      961 cgtcaaacaa taggttcttt agaaaatgga cgttataatc cctctattat attggcattt
     1021 aaaattgcaa ggtactttca aatgagcatt gaagaaattt ttatctatga ggaggaaagt
     1081 gaagatgaaa aataagggtc agatttggta tatcggttat attgtttcgt tgtttttgat
     1141 tgtgacaatt ttggttacag attttcctaa aatggtagat attggacttg ctattttatt
     1201 cagtgccgtt tttggcgttt ctcatgttca aattttgcat gataaaatga ttaaaaatga
     1261 tgcagactac aaaatcaatg ttatggacga acggaatatc ataatcaaag aaaaagcggg
     1321 aaatataaca aatatgttta acacaatact gttaggggtg gcaacagtta tatttatttg
     1381 tttagactac atcattccag ctattattgt cggaagtatc attgcaatcc agccgattat
     1441 tttaatcatt atcagtagtg tgcttgagaa aaaaatgtag aaataaaggt tcatacaaag
     1501 gttctgttgc aaagttttcc aaaaaatcta ttttagtgta aaattgagaa aaaagacaga
     1561 gaggacagag taatgaatca ttttaaaggc aaacaattca aaaaagacgt cattattgtc
     1621 gctgttggtt actacctgcg ttacaatcta agctatcgtg aagttcagga attgttatat
     1681 gatcgtggaa taaatgtttg tcatactacg atttatcgtt gggtacaaga gtac
//
LOCUS       DACCXO010000216         1666 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.224, whole genome shotgun sequence.
ACCESSION   DACCXO010000216 DACCXO010000000
VERSION     DACCXO010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.224"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            40..867
                     /gene="panB"
                     /locus_tag="IVY70_002872"
     CDS             40..867
                     /gene="panB"
                     /locus_tag="IVY70_002872"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAP7959950.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            882..>1666
                     /locus_tag="IVY70_002873"
     CDS             882..>1666
                     /locus_tag="IVY70_002873"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAP7959951.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLV"
BASE COUNT          648 a          169 c          361 g          488 t
ORIGIN      
        1 attagaaaag tatttaggag agagttagga gagaaataga tgaaaaatac agcagttaca
       61 tttaaagaat ctaaattaag aaatgaaaaa ttaacaatgc ttacagctta tgactattca
      121 acagcaaaaa taattgatga agctgggata aatggaatat tagtaggaga ttctcttggt
      181 atggtatgtc taggacatga agatacactt tcagtaacaa tggaagacat gattcatcat
      241 actagagcag ttacaagagg agctaagaac actttagttg ttgcagatat gccatttatg
      301 tcttatcaaa cttcagtata tgattcagta gttaatgcag gaagattaat aaaagaagga
      361 cgagctcagg ttgttaaatt agaaggaggg atagaagtat gcgataaaat agaggctata
      421 gtaaaggctt ctatacctgt tatggcgcat ataggtttaa cacctcaatc tgttaatgca
      481 tttggaggat ttaaggtaca aggaaaggac aaggaagctg ctaaagaatt aataagagct
      541 gctaaagctg tagaaaaagc aggagcattc gctgtagtgt tagaatgtgt tccaactaag
      601 cttgcagagt taatttctaa agagatatca atacctacaa ttggtattgg agcaggtgca
      661 gggtgtgatg gacaaattct tgtatatcaa gatatgttgg ggatgtatag tgattttacc
      721 cctaaatttg tgaagaaata tgctaattta tcagaagaga tgaataaagc atttacaaaa
      781 tatatagagg aagtaaaaga tggagttttt ccaggtccag aacatgggtt taaaataagt
      841 gatgatgttt tagaaaaatt atattaaaag ggtcaagaaa tatggttata tttaaaacag
      901 tgaatgatgt tagaagtcaa gtaaaagagt ggaaaaaaca aggcttaaag gtaggattag
      961 ttcctactat gggatattta catgaaggtc atgaaagttt aattagaaaa gcatcaaaag
     1021 aaaatgataa agttgttgta agtatctttg taaatccaac tcagtttggg aaaaatgaag
     1081 atttaggtag ttatccaaga gacttagaaa gagatataga agtatgcaca agaggtagag
     1141 caacagcaat atttaatcct gaagttgaag agatgtattg tgacaatgca agtacttttg
     1201 ttaatataac tggattaaca gaaggtttat gtggagctag taggccaata cattttagag
     1261 gggtttgcac tgttgtaagt aaattattta acataatacc agcagataga gcttattttg
     1321 gagaaaagga tgcacaacag ttagctgtaa ttaaaagaat ggttagggac cttaatattg
     1381 atatagacgt tgtagggtgt ccaattataa gggaagagga tggactagct aaaagttcta
     1441 gaaacacata cttaagttta gaggaaagat cttcagctac tattttaaat aaatctttaa
     1501 ctttagctaa ggaagcttta aataatggag agagagacag cttaaaaata attgagataa
     1561 taagtaaaaa tataaatact tataatttgg caaagattga ttatgtagaa gttgttgatt
     1621 ctttatcatt acaaagagta aattatattg aaaaatcagt tttagt
//
LOCUS       DACCXO010000217         1634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.225, whole genome shotgun sequence.
ACCESSION   DACCXO010000217 DACCXO010000000
VERSION     DACCXO010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.225"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..1350
                     /locus_tag="IVY70_002874"
     CDS             <1..1350
                     /locus_tag="IVY70_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7959952.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..>1634
                     /locus_tag="IVY70_002875"
     CDS             1484..>1634
                     /locus_tag="IVY70_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7959953.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIK"
BASE COUNT          644 a          246 c          347 g          397 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaag
//
LOCUS       DACCXO010000218         1525 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.226, whole genome shotgun sequence.
ACCESSION   DACCXO010000218 DACCXO010000000
VERSION     DACCXO010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.226"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            469..>1525
                     /locus_tag="IVY70_002876"
     CDS             469..>1525
                     /locus_tag="IVY70_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAP7959954.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREK"
BASE COUNT          545 a          288 c          269 g          423 t
ORIGIN      
        1 aaaaaagttc aaaagatcaa gtgtgcgctt cgctcccact cccctcgacg ttttagtagc
       61 ctttccctca cttcgttcag tccaagccaa ctaaaaagtt ttcgggatac attctccttc
      121 tcttcctaat aatcaattaa aattttaatc tttatatttc tgctaatcac tcaggaaaca
      181 gtacagaaaa acaaacacgt atcatagata taaatgtaat ggcatagtgc gggttttatt
      241 ttcagcctgt atcatagcta aacaaatcga gttgtgtgtc cgtttttggg cgttctgcta
      301 gcttgtttaa agtctcttga atgaatgtat gttctaagtc aaaaaactct gacagcgcct
      361 ttatatagat aaagtccgta taattgtgta aaagtaaaaa ggccatataa cagtcctttt
      421 acggtacaat gtttttaacg acaaaaacat acccaggagg acttttacat gacccaagta
      481 cattttacac tgaaaagcga agagattcaa agcattattg aatattctgt aaaggatgac
      541 gtttctaaaa atattttaac aacggtattt aatcaactaa tggaaaatca acgaacagaa
      601 tatattcaag caaaagaata tgaacgaaca gaaaaccgac aaagtcaacg aaatggctat
      661 tatgagcgca gctttacgac acgtgtaggc acgctagaat taaaagtacc cagaacacgt
      721 gatggccatt tttcacccac agtgtttgaa cgttatcaac gaaacgaaaa agccctcatg
      781 gcttcaatgt tggaaatgta tgtatcaggc gtttcaactc gtaaagtatc aaaaattgtg
      841 gaagaacttt gtggtaaatc cgtctctaag tccttcgttt ctagcttaac agaacagcta
      901 gaacctatgg ttaacgagtg gcagaatcgt ttattatcag aaaaaaatta tccttactta
      961 atgaccgatg tactctatat aaaagtacga gaagaaaatc gagtactctc aaaaagctgt
     1021 catatagcga ttggaataac caaagatggc gaccgtgaaa ttatcggctt catgattcaa
     1081 agtggcgaaa gcgaagagac ctggacaaca ttttttgaat acctaaaaga acgcggttta
     1141 caaggtacgg aactcgttat ttctgatgcg cacaaaggat tagtctctgc cattagaaaa
     1201 tccttcacca acgtaagttg gcaaagatgc caagttcact tcctaagaaa tatctttacc
     1261 accattccta aaaaaaattc aaaatctttc agagaagctg ttaaaggaat ttttaagttc
     1321 acagatatta acttagcgcg tgaggctaaa aatcgattga ttcatgatta tatcgatcaa
     1381 ccaaaatatt caaaagcttg cgcatcattg gatgatggat tcgaagacgc ctttcaatat
     1441 accgtacaag gaaattccca caatcgacta aagagtacca atctaattga acgactgaat
     1501 caagaagtac gcagaagaga aaaga
//
LOCUS       DACCXO010000219         1479 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.227, whole genome shotgun sequence.
ACCESSION   DACCXO010000219 DACCXO010000000
VERSION     DACCXO010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.227"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            131..505
                     /locus_tag="IVY70_002877"
     CDS             131..505
                     /locus_tag="IVY70_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP7959955.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IVY70_002878"
     CDS             complement(619..1248)
                     /locus_tag="IVY70_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7959956.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IVY70_002879"
     tRNA            complement(1382..1454)
                     /locus_tag="IVY70_002879"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          500 a          225 c          260 g          494 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataatagga
//
LOCUS       DACCXO010000220         1459 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.228, whole genome shotgun sequence.
ACCESSION   DACCXO010000220 DACCXO010000000
VERSION     DACCXO010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.228"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(762..835)
                     /locus_tag="IVY70_002880"
     tRNA            complement(762..835)
                     /locus_tag="IVY70_002880"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(799..801),aa:Ile,seq:cat)
     gene            complement(1025..>1459)
                     /locus_tag="IVY70_002881"
     CDS             complement(1025..>1459)
                     /locus_tag="IVY70_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7959957.1"
                     /translation="TDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGEIENA
                     NLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSFN
                     MRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          443 a          265 c          263 g          488 t
ORIGIN      
        1 ctgcgcgagg cttaccagcg agatagagat cgacgatttg ttgtttgaat tcttttgaat
       61 aggttcttcg ttgacgtctt gacatgaaaa atcctccagt gtgtttttta ttattctaca
      121 caccttattt tttctgtcta gtctagtgta gccgattcat gactgacgtg ccggacgcac
      181 gtagcgatca tcggcgttat gactatataa agtatacttt cgttgatcca gaaaggtata
      241 aagattaaag taggctacct aaaaaggtag cccggaacga attttatcac catacttgtg
      301 aaaggagata ttttttaagt tagtattaag attgtgtaat atgatgatat ctatatttta
      361 tagtatcagt gctataaaat caaaaataag tcactaatta actaccactc caattgtaag
      421 ccttttttct cacttttttt caaaaatatg gtatgctttt taatggcttc aaatataaaa
      481 gagtttaaag cgtaacacac ttatggggaa gtggtttggg gtgcgcttta aactcttctt
      541 tattattatc tcacaattta acccaaatgt ctttctattt aaaaatcaaa gtaaaacttt
      601 tcaaatatac agaagtataa ctatgtgaaa catcctttca ttaatccata aaaggataca
      661 taaaaaagcc actcatttga gtggcaatga agaaaagctt tagcttgtat aatactcttc
      721 aaaaaaattc taacacagaa cgattcaaat ggctacgtta atgtaccctg taggactcga
      781 acctacgacc ggacggttat gagccgtctg ctctgaccaa ctgagctaag ggtactggtt
      841 gttgccacat aaagccataa acaatcaacc agtagaatgt gtggcaacaa acctgttatc
      901 gcatagcttg gagtgtgact atttatgggt gatagtgaag atatgcgata acatcactat
      961 tttatcgaat aatttttata gttgtcaata tagttatgta ctgctcctca acgaggagct
     1021 atttttatcg tttaggaata tttaaatacc aacgtttgtc atggaaatct tgcgcaccgc
     1081 ctttagtgtt tccctctgga tcattcgttg cccgcatcat tacatagact ttcttactag
     1141 gaaaattacg catattgaaa gatacatgat aaccaacatt tccagaagta ttataagctt
     1201 ggtttacatc tggtctataa attccatcag ctcttactcg agctaattct ttcccagtat
     1261 tgtagtccat aatgaaaata tactcgtatt tatagttagc aatgtgccat ccagccacat
     1321 gcaagtttgc gttttcgatt tccccaaact gatcaatgtg ggcgtgattt gttccatctg
     1381 tcagcgtagg attagctgca ccagctcgtg ttggatcaat gacaggcttg ttttcagaag
     1441 ttgttggatt ttcatcggt
//
LOCUS       DACCXO010000221         1459 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.229, whole genome shotgun sequence.
ACCESSION   DACCXO010000221 DACCXO010000000
VERSION     DACCXO010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.229"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..787
                     /locus_tag="IVY70_002882"
     CDS             <1..787
                     /locus_tag="IVY70_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7959958.1"
                     /translation="PFLTKSEKKVADFILNSGKTIIYSTMSDIKKNTKVGDATIIRFC
                     QKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYSTIRLLDEEKLNE
                     AIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQAQVASLLTVNDL
                     VIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQTAIEEFLNGGS
                     LAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1017..1247
                     /locus_tag="IVY70_002883"
     CDS             1017..1247
                     /locus_tag="IVY70_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP7959959.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
BASE COUNT          505 a          211 c          231 g          512 t
ORIGIN      
        1 tccattttta acgaagtctg aaaaaaaagt cgctgatttt attttaaatt ctgggaaaac
       61 tattatttat agtacgatga gtgatattaa gaaaaacaca aaagttggcg atgctactat
      121 tattcgcttt tgtcaaaaaa ttggtttttc tggtttttct gatttaaaaa ttgaaattgc
      181 aaaggaagac tttagtcaaa aaaaagaaca tccgactagt gtaaagtact atgatgaaat
      241 tgctaatagt ttaacagaag ctctctattc cactattagg cttttagacg aagaaaaatt
      301 gaatgaagcc attaaaatga tcactcaatc aaaaaacata tatatatttg gagtgggatc
      361 tagtggaaat actagtcttg atttagaaaa tatgtttctt cgcgtaggag ttcaagcaaa
      421 agctgtacta gaccctcatt ttcaagccca agtcgcttct ttattaacag ttaatgattt
      481 agtaatcatt ttttctttat ctgggaaaac gaaagatact tatgattcat tgaaaatagc
      541 taaaaaaaat ggcgcaaaaa ttattgctat aactaactat attcattcac ctataggaaa
      601 gtctgcagac ttagttttac aaacagccat tgaagaattt cttaatggtg gatctttagc
      661 tggaaaaata tcacaacttt atatttgtga tctattagtt catggttatg aacaaaacaa
      721 taatattgat tcagttgaat taagagaaaa agttttgcgc tctattattg ataaaagtat
      781 tgagtgaact acccccactt agctaaacct aacggttttt aacgattgaa gtaggcaatt
      841 cttgggatga gagcatactt gatagcttat tttttactaa tagcggtggc tcttattgac
      901 caaatatccc tgtagttcct acggttcttg attttatcta aagatatttt tatcgtggaa
      961 gctattgatc aacttgaaag aaaaacgtgt agctcattat cactagttta tcgagtatgg
     1021 atgaagcaat ggatcatcct ctcttaaata aatttattaa ggatctgatc cttcaaattt
     1081 tttccatgat cgatgagcag gaacctactg aatctaaacg acatcgggca caaggaatta
     1141 ttaagattgc taagcgaatg ttttctataa agggtgttct aagctataca gtgttaattc
     1201 aaaagattcc caatcatctt gtttataaga acaacgttga agattaaaaa agtgtttcaa
     1261 tgctaaaatt gcaaaatact tcaatgttat gagacaaact gtctataaga atcaatttcc
     1321 acccatttca tcacttgtta aaagtttatg atccttttga tttttatatt aatttatctg
     1381 tatttaatac agattttatt tattagttca tctgcctatt gtattttatt ttgttgcctt
     1441 accatctgaa gtaaatata
//
LOCUS       DACCXO010000222         1447 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.230, whole genome shotgun sequence.
ACCESSION   DACCXO010000222 DACCXO010000000
VERSION     DACCXO010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.230"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(417..1031)
                     /locus_tag="IVY70_002884"
     CDS             complement(417..1031)
                     /locus_tag="IVY70_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7959960.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          473 a          277 c          242 g          455 t
ORIGIN      
        1 gaaaaaggta tcctgttgga caattttgtt atatcaggta aacttcattg caataatgat
       61 aacaaatgta ctataattac tttgtaaaga gttttgggga agactctttc atcaacgtgt
      121 attaagagac aagaaagaat ccatatggat tctttcttgt ctctttttcc actacttttt
      181 atttttctga cactacatat tcttataact catttctcat aactgtgaaa caacttataa
      241 aaataacaaa ctttaaacaa aacaagctta aaatcaaaga tgatccatct taaaataact
      301 aaaacaaaaa aagtagtcgt tgttaatcct cattaataat cgttttcttt ttttcaaacg
      361 ctccattttt atgtggaaca ttattttttt aaggaacttt gtcaacatgt ggataactat
      421 tttccacttg tgtcctggac tagctgtttt gctgtaaaat acaaaacttg ctgcagcaaa
      481 aagatcgttg caagagaggc tgttccgcag atcgctggat ctagatttgg caaagtcaag
      541 actgcatcat gtaaattggc gtggttgtcc aaaaccaaat cagcaaattc aaataatcgc
      601 aaaccactgc tatgttgcga tttgatactt cgtgacaaat caaatccagt aacaatgatt
      661 aatggatgac cattttcttt gatccactga gctagttcga tgattgccgg gtttcttcct
      721 tcattcgaaa gaagaagaaa aatgtcttct gatttgacag aaagctgatc aataattgcc
      781 tgtcctgcac cttctagcgt ttcatagaca cctccggctg gattttcaat caatatactt
      841 ggtacaagat ccggggtttg atcccaaaat tcaaatgcta caccacttag tgtgcggcta
      901 gcatatatct gcaatctacc accttgatta atttgtttcg cgatccaagt gccagcttga
      961 agcatcagcg gctcttctat ccttaatttc tcttccaatt ggtgataata gtcaaaataa
     1021 cataaactca tttatctctc ccgcctagct atacttgaca tgaatagaaa cagctgccta
     1081 atctaaggaa gtacataaaa aaacagtagt tagacagtaa cacatgtggt ctttgctgtt
     1141 tcaaacacaa aaaaggcatt gaacgaatac gcccaatacc ttgacatagc aaataaaaca
     1201 acgtgcagac catggaaaat atctacatag gggtagaatt ttcaccctag attaggtaca
     1261 ataaattagg tcgatgcact gcattgacta tggcaaaatg atcggtcagg gtgtatgtgt
     1321 gtgactggtc attccctgat tcttagcgcc aactaagggt cagggagcct ttttttattc
     1381 agctgtttct atctgaattt attgtaagta atctcttatc caagtgcaag caagaaaata
     1441 caaaaaa
//
LOCUS       DACCXO010000223         1424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.231, whole genome shotgun sequence.
ACCESSION   DACCXO010000223 DACCXO010000000
VERSION     DACCXO010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.231"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(150..1337)
                     /locus_tag="IVY70_002885"
     CDS             complement(150..1337)
                     /locus_tag="IVY70_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP7959961.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACCXO010000224         1407 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.232, whole genome shotgun sequence.
ACCESSION   DACCXO010000224 DACCXO010000000
VERSION     DACCXO010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.232"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..>1407)
                     /locus_tag="IVY70_002886"
     CDS             complement(<1..>1407)
                     /locus_tag="IVY70_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959962.1"
                     /translation="IYTKNLTGLDLEIDAQSVLVGVYPFISSSSEGEDEITLPEEVIF
                     TDYVDDYPAGYVSFVDFKDKATDVATLREAAKDWLKTNIDKQKPQVSGSIELVPLRHQ
                     RGYEKFVDLEKVSMGDGVDVYHPQLKVNMSARIVEYTFNVLTNSYDKLVVGNVKTNFL
                     ENTENNVSNLINDAIDQLKNGGEISDLLNDIVDHQTDMITGQNGGYVLLDPKEAPSRI
                     LIMDTPDKNTARNVLQINNAGIGFSKTGINGTYETAWTLDGGFNASFITAGEIVGITI
                     RGTTLISDGADYRTSIANGKMTWYSKKVNKDIMELEARDYVSADAGIVSYTMKTGGGF
                     MIRNPQGNLVFSTWDNGNNRPFLSFGAPNFRYSNASYVTSGDGSSLSINGSAGNSWEF
                     KVAGRTMKFTSDGMLTLPGCFFGSWEDGKLARFEQSTVQVYKDFTVRGTKNSTVPTEH
                     YGQRLLNAYETPEYYFADY"
BASE COUNT          432 a          308 c          198 g          469 t
ORIGIN      
        1 ataatcagcg aaataatatt ctggagtttc ataagcgttc aatagtcgtt gtccataatg
       61 ttctgttggt acagttgagt ttttagtacc tctaacagta aaatctttat atacttgtac
      121 cgttgattgt tcaaacctag caagtttccc atcttcccat gaaccaaaaa aacaacctgg
      181 taacgttagc ataccatcac tagtaaattt catagtccta ccagctacct taaattccca
      241 tgagttaccc gcactaccat taatgcttaa agaactaccg tcgccagaag ttacataact
      301 agcattgcta tacctgaaat tgggcgcacc aaaagataga aacggtctgt tattaccatt
      361 atcccacgta ctaaaaacca agttaccctg tggatttcta atcatgaaac caccaccagt
      421 tttcatggtg tatgatacaa taccggcatc agcacttaca taatcacgtg cttctagctc
      481 cataatatct ttgttaactt tttttgagta ccaagtcatt ttgccattag caatacttgt
      541 tctataatca gcaccatcac taattaatgt agtacctcta atagtaattc ctactatttc
      601 accagccgta ataaacgagg cattgaatcc gccatctaac gtccatgccg tttcatatgt
      661 tccattaatg ccagttttag aaaaaccaat accagcattg ttgatttgta aaacattcct
      721 tgcggtattc ttatctggtg tgtccataat caaaatacga ctaggcgctt ctttaggatc
      781 taataaaaca taaccaccat tttgaccagt aatcatatca gtttgatgat ctacaatatc
      841 attgagtaaa tcactgattt cgccaccgtt tttcaattga tcaatggcat cattaatcaa
      901 attgctgaca ttattctctg tgttttctaa gaagtttgtt ttgacgtttc ctacaactaa
      961 tttatcgtat gaattggtta gaacattaaa cgtatattcc acaattctcg ctgacatatt
     1021 cacttttaac tgtggatgat acacatctac tccgtcaccc atcgaaactt tttctagatc
     1081 aacaaatttt tcatagcctc tttgatgcct caatggtact aattcaatcg aaccactcac
     1141 ttgtggtttt tgtttatcta tgtttgtttt caaccagtct ttagcagctt cccttaatgt
     1201 ggctacatca gtcgctttgt ctttaaaatc aacaaaagaa acatatccag caggataatc
     1261 atccacgtaa tccgtgaaaa taacttcttc tggtagagtg atctcgtctt ctccttctga
     1321 agagctgcta atgaatggat aaactccaac taaaacactt tgagcatcaa tctctaagtc
     1381 aagaccagtt aagtttttag tataaat
//
LOCUS       DACCXO010000225         1402 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.233, whole genome shotgun sequence.
ACCESSION   DACCXO010000225 DACCXO010000000
VERSION     DACCXO010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.233"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            68..643
                     /locus_tag="IVY70_002887"
     CDS             68..643
                     /locus_tag="IVY70_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7959963.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(712..>1402)
                     /locus_tag="IVY70_002888"
     CDS             complement(712..>1402)
                     /locus_tag="IVY70_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7959964.1"
                     /translation="TPDVIIGRAERTFSCSVSTLYRRFKTGEFNVLHLPMQGKRKPNG
                     YKEKRGKQAFKRNISERKKDYVVFEEEFGHLEGDTIVGIHHKSAVITLVERLSKAIIA
                     LKPEGRKAVDIENSINEWLQSVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPG
                     TPSQRGLNEHSNGLLRKDGLPKEMDFNQVNQGFISSVASKRNHIPRKSLNYQTPLEVF
                     LSYVNGKFCLA"
BASE COUNT          380 a          254 c          288 g          480 t
ORIGIN      
        1 gttcttttaa atgcacaaga agaaacgatt atgaaagatt tggaaaggtt aaagatgggc
       61 taacaaaatg aataaaaaca aaatggcaac tcgcgtactt acagtgatgg ctttgtgtat
      121 tgggatcaat tatattggag gaacgattgc tttatggctt cgtttgccaa tctatctcga
      181 ttctatcgga acgatttttg ctggtgcatt gcttgggcct gtacctggaa tgctgacagg
      241 attgtccagc ggtattttaa gtggagtgac tacagatatt ttttctctgt attattcgcc
      301 aatccaaatt ctcacaggct tgcttgctgg attgattttg ccaagatatc tcggcaaaga
      361 taaaaacagc ttgaaactgt tattactcgc tttgattttg tcggtacctg gaacgatcgt
      421 ttcttcgatc attaccgttc agttatttgg cgggatcacc tcctcaggat ccagtatgat
      481 cgttcagcta ttacatgggt taggtttaag tcagacaaca agtgtgatct tggtacaggc
      541 gggtacagac taccttgatc gcttgttgtc tatcgtcgtt gtttcattag tcatcactat
      601 gttgccccaa cgcttgatat attcaaaaag aaaaataggc tgaataaaca gagctggatt
      661 agcaaaataa tgctaatctg gctctgtttt ttcattttat tatttgtcaa attaagcgag
      721 acaaaacttt ccatttacgt aactcaaaaa aacttctaat ggtgtttggt aatttagtga
      781 ttttctaggg atatggtttc ttttggacgc aacggatgag atgaatcctt gattgacttg
      841 gttgaagtcc atttcttttg gtagtccatc ttttcggagg agtccgtttg aatgttcatt
      901 taatccccgt tgggaaggcg ttcctgggtc tgcgaaataa atatcaatat cattcgtatt
      961 actaatactt ttccaattag agaattcttt tccacaatca aaggtaattg atttgaaaag
     1021 atttttgggt acggattgaa gccattcatt aatcgaattt tcaatatcaa ctgccttacg
     1081 gccttctggt ttcaaggcga taatggcttt tgaaagtcgc tcaacgagtg tgatgacggc
     1141 acttttatgg tggatgccga caatcgtatc accctctaaa tgtccaaatt cttcttcgaa
     1201 aacgacataa tcttttttac gttcagaaat atttcttttg aatgcctgtt ttccacgttt
     1261 ttccttataa ccatttggtt ttcgttttcc ttgcatcggt aaatgtaaaa cattgaattc
     1321 tcccgtctta aaccggcgat agagggtact tactgaacaa gagaaggttc gctctgcacg
     1381 gccaataatg acgtctgggg tc
//
LOCUS       DACCXO010000226         1397 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.234, whole genome shotgun sequence.
ACCESSION   DACCXO010000226 DACCXO010000000
VERSION     DACCXO010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.234"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            25..786
                     /locus_tag="IVY70_002889"
     CDS             25..786
                     /locus_tag="IVY70_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7959965.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(901..1341)
                     /gene="fabZ"
                     /locus_tag="IVY70_002890"
     CDS             complement(901..1341)
                     /gene="fabZ"
                     /locus_tag="IVY70_002890"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP7959966.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          465 a          269 c          263 g          400 t
ORIGIN      
        1 atagtaaatt ggaaaggaac agttatgaag aaaaataaag ttattttaag tattagttta
       61 gcagcagcat tgctgtttgc tgctggttgt tctgcaaaca acacgacttc ttctagttcg
      121 tctacagaaa gttcaaaaag tacatctact gcggaaagca cgactgccag tagcgtcgat
      181 ttgaacgcat tggaattacc acaattgacg aaagatgtcg ccgaaaatga agatctggtc
      241 gaaatggtga cgaataaagg cacaatcaaa atcaaattat ttcccgaaca cgcaccaaaa
      301 gcggttgaaa actttatgac acatgcaaaa gacggttact acaacggact gactttccat
      361 cgcgtcatca aagatttcat gatccaaggt ggcgatccta aaggcgatgg cactggaggg
      421 gaaagcattt ggggagaagg atttgaaaca gagatcaaca accaccttta taatataaga
      481 ggtgcgttat ctatggctcg ctcacaagat cctaacagta acggcagcca gttctttatc
      541 gttcaaaata cagatgatat gcatgatggt cttttaaaag acgactatcc tcaagcaatc
      601 atcgacgctt ataaaaacgg cggttatcct tcactagatg gccattatac tgtcttcggc
      661 caagtgattg aaggaatgga tgtcgtggat gctattgcaa atatcgaaac agatagttca
      721 gacaaaccaa aagaagaagt aaaaatcgat aaaatcaata tactgcaaga agcaaaacaa
      781 aaataaaaaa ataacgtctg aatcctgcat agatgacaac gtatgctagg attcaggcgt
      841 cttcttttta cataaaaagc aagtaagctt ccttttgttt tgctatatca ggttttatag
      901 tcaagcattt tggttgacga tgaacgtgaa ctcgcactca cagacttttt tatcatctac
      961 atatgctgca gataacgctg taccgactga tcccttcatt ttggtgattt caaaatgaca
     1021 tttcagcaca tctcctggaa caactttttg acggaatttg gccttattga tcccaccgat
     1081 ataagctctt tttcctagaa actcttctga ttttaggata agtatcgaac ctgcttgtgc
     1141 cagtgtttct agaatcaacg cacccggcat cactggattg tcaggaaagt gaccttggaa
     1201 aaaatcttca ttgattgtta cattttttgt cgcaacgatc ttttttcctg gttctaaagt
     1261 atctacataa tcaatataac aaataggata acgattagga atcaattcca ttacttcagt
     1321 agcagataat acgtaagaca taattaaact ccttctttca ggcaattgcc attttcatta
     1381 ctttgactat caaagta
//
LOCUS       DACCXO010000227         1397 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.235, whole genome shotgun sequence.
ACCESSION   DACCXO010000227 DACCXO010000000
VERSION     DACCXO010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.235"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            12..422
                     /locus_tag="IVY70_002891"
     CDS             12..422
                     /locus_tag="IVY70_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP7959967.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            436..792
                     /locus_tag="IVY70_002892"
     CDS             436..792
                     /locus_tag="IVY70_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP7959968.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          511 a          209 c          263 g          414 t
ORIGIN      
        1 ggagtgagaa aatgataaaa gtatatactt cgcctagctg tacttcctgt agaaaagcaa
       61 gagcatggtt gcaagcgaat caacttgaat ttgaagagaa gaatattttt gccgaaccgt
      121 taacagaaag tgagatcaaa aagatcttgg cagcgactga aggaggagtt caagatatta
      181 tttccactcg gtcgaaaatt tacgaaaagc tgggcataga ttttaacgaa ttaactttaa
      241 agcaaatgat cgtcttattc aaagaatatc cttcattatt gagacgacct attttgattg
      301 atgaaaaaag aatactaatc ggatttaatg aagatgagat ccggtcattt gtccctcgtg
      361 aaattcgatg tgttgccaga aatctgatct atttacaaaa caataacatc ttaaattcat
      421 agggagggag ccttcgtgga agtgaaagtc tatggatcag cgcactgtgt aacaagtctg
      481 aaggcgaaac aatggttaga acaccatcaa ttgccttatc aatttgttga cttagaagaa
      541 cgcgatctaa gtacagaaga agcagaagaa ttatgcagtt ttgaagaagt gcaagcagaa
      601 cagctatttg cgacttggtc tgaggctttt cacaacattg cgattgacct ttcctgtgta
      661 ggaaaaaaac aactgatttt tttatgcaca caacatacca atctcctcag acgtcctttg
      721 attattatcg atgaagttct atttattgga tacaatgagc agttgatgga acagcttatc
      781 ttaaataaat agactttaac tgttttaatc aagtcactga gcatttcata cagttgtaac
      841 agtcgaacac ttagcatcaa gcgtgttatg tgttcttttt ttgactttat ttttctacta
      901 tagagacatg ctcaatttat gtggtcaaaa ttcaaatcta ggtggggata tagagttaaa
      961 aatatattga catgtgtaaa tttacatgat atattcattt ctgttgtttt aagtagcaaa
     1021 gcaataaaat agtattttaa aaaacatata aaatagttgt tgacatgtta gaaatgacat
     1081 gataagataa ctgagttgtt aaaaaagaac cacttgtgaa gcttgattct atcgaaaaaa
     1141 acttcaaaaa agttcttgac aaataacaaa tgataagtta tgataaatga gttgctgtaa
     1201 gatttcgaaa aacgaaatca aaaaaacttt taaaaaagag ttgacaatta cttgtctctc
     1261 tgatatgata taaaagttgc tacggcagcc aaaaacgaat cggggaacac tcctgatgaa
     1321 gtagaccttt gaaaactgaa caaagtaaga caaaccaaat gtgtagggcg tcttgattca
     1381 attcaagaca acaaaca
//
LOCUS       DACCXO010000228         1385 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.236, whole genome shotgun sequence.
ACCESSION   DACCXO010000228 DACCXO010000000
VERSION     DACCXO010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.236"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(165..464)
                     /locus_tag="IVY70_002893"
     CDS             complement(165..464)
                     /locus_tag="IVY70_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP7959969.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            815..>1385
                     /locus_tag="IVY70_002894"
     CDS             815..>1385
                     /locus_tag="IVY70_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP7959970.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNC"
BASE COUNT          468 a          220 c          195 g          502 t
ORIGIN      
        1 atactagctg tttttgtgtt aattaaagga taaaatacaa tataaagaaa catctgtttc
       61 tttatttaga ataaaaaaat acagactctg ttcaaataaa tgaacctagc ctgcattatg
      121 agattgacta tactttttaa ttcattgaga tgaataactc gtttttataa atgcccaaaa
      181 gtaatcggtg ccgaagccat tcctcgacct aattcttttt caaattttcc tattgttgat
      241 atctccatat aaaacggtga tactggttta gacaattctc ccctgattgc taacggacgt
      301 aaactacgtt ccaaattatt tatgacttta ggataatact gacctttttc taagcctgtc
      361 tttgcgtcta tcaaaagtat acgttccttt tctttgatct ctggatttaa aacgagatca
      421 tataatttta gtaccatgtt ttctatttct tcacttttag acatcacgat ttcctcccaa
      481 aaatatattt cttcccacta cattctataa acaaagtata cgctacatct agattttttt
      541 aaatcattac tggattgtta aaacctttca atacactgtt atggcaacga tttttatttt
      601 aaaaacattc taaattaaat cagtaaaaat ttttattcta agatttttat tagaattacg
      661 cctaatttgt gtctctcagg gcaattctaa gaacgtttca agagatagcg acactatcgt
      721 tatcgaaaaa ggcgaaaacc tatctcattt tctgctaatg taagttggtt tagcttttgt
      781 taatatgact ttatgaaaat ggaggtaatt aaatatgatt tttcctaaag ttaatgaagc
      841 aagcttgaat aaaggatcct tttttcatat ctatgaaaac gaagattttg aactgtataa
      901 tatcctttca caaaaagagg tcatcgaaaa ccaaataatc tgccatccaa ttatttacaa
      961 atctattttc aagtatgcat tttttgcttc caatgatttt caaagaatgg agattaccga
     1021 ctgtatattc gaaaactgtg atttttcagg ctgtatattg acaaaaagca tgttgcatcg
     1081 cgtgaaattt gtcaattgta aattaacagg tactaaattg atggaaagtt atattgggaa
     1141 cacgttattc gagaattgta aaatggacta tgtaaattta tctggttcaa acataaaaga
     1201 aagtaccttt gagcactctg tcttatcaag cgcagatttt gtagactgtt ccctcactaa
     1261 aataatgttt tctactaatg atttagaaaa tgctagcttt tttgatactg atttaaaaaa
     1321 tatcgattta agtagcaact cctttgaaat gattgaagta cgctcggaac atctaaaaaa
     1381 ttgta
//
LOCUS       DACCXO010000229         1381 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.237, whole genome shotgun sequence.
ACCESSION   DACCXO010000229 DACCXO010000000
VERSION     DACCXO010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.237"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..581)
                     /locus_tag="IVY70_002895"
     CDS             complement(<1..581)
                     /locus_tag="IVY70_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959971.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAISSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLG"
     gene            complement(591..1328)
                     /locus_tag="IVY70_002896"
     CDS             complement(591..1328)
                     /locus_tag="IVY70_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7959972.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
BASE COUNT          422 a          278 c          209 g          472 t
ORIGIN      
        1 cctagacttt tttcatgacg taatgtgtta ttatctttta gaaactcacc atgaaatcga
       61 tctagaatag atccctcttt tccaccaaag aattctaaaa aattcgcctt ttctatcttc
      121 acattagcaa gcgtatctac taatgacgag aaagaaaact gcgaagagat agctggtttc
      181 gctaaaactt ttgcgttttg ccatgcctga gtagcagtga tcttttctgt tccgctgtca
      241 tatttattca acaccgattt tcttatatca ttgaaaatag gttcagcttt tacttctatc
      301 gtattgccta ttacagaagt ttttgcataa taaatccgta gacgctgttt tgctcgattt
      361 tcatctacat aacactgaat aatacgtccg tctacaatca aatctgcatt agttccgttt
      421 attgaataag taccctgaaa tatctcggct ccgtttagtt tattgctaac agtagctgtt
      481 aaccagtctg acaaagcgcc taaaccttgc gtatcatata aatgttcagc taaattattc
      541 gcgtcatttt tatcgtaaat agttattaaa ttatcgatca tctatttcac ctaccttaac
      601 ccgttacgat aaatttgtat tttgctcaaa ccagtgcaat taaaatgatt gatatccact
      661 tgcaatgtcg gatattgcat ggtcttcatt ttgttggacc gatctaaaat atctccgtcc
      721 gattgctctt cgtagcaaag catcaaatca ctatcaatga ctacatcagt tcctgctact
      781 aagccttcga aactaaacac ataatcattt aagatgaact ggcatgaagt agctgaagga
      841 gtgatgataa tctttggaaa actttcttct aaactattat tcagcaagtt aaatgactgt
      901 ggtttatcta cggttatagg tacatcttct tgaactcttg cgaatggttt cgcagtaata
      961 tttacatcga actctcccca ttcaacaata tcgttttctg catccccaat atcgatagtc
     1021 tggataacgt aatagacgtt gggatcgtca gagaattcta atttctttgc atagtttaac
     1081 caatgacgca tgatataaaa cgattgcttg aacgcttgat ggtcttccac atcctctaaa
     1141 tagttatagt gcaatgtaaa cgacatatct tcaaacgagt aatcttgtac taagccacct
     1201 aacctaccta aaacagaagt ttcaactctc tgtctttttg gagaaggtat ggttggtctt
     1261 tcagctaaag ccaatttatg caaataatca ggaaatccat cgattataga atgtatacaa
     1321 tcagtcattt tttcacatcc tttttaatac taaaaaaaca ggagaaatac tctcctgttt
     1381 a
//
LOCUS       DACCXO010000230         1356 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.238, whole genome shotgun sequence.
ACCESSION   DACCXO010000230 DACCXO010000000
VERSION     DACCXO010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.238"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            82..>1356
                     /locus_tag="IVY70_002897"
     CDS             82..>1356
                     /locus_tag="IVY70_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP7959973.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLIN"
BASE COUNT          461 a          261 c          257 g          377 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaat
//
LOCUS       DACCXO010000231         1334 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.239, whole genome shotgun sequence.
ACCESSION   DACCXO010000231 DACCXO010000000
VERSION     DACCXO010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.239"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            164..715
                     /locus_tag="IVY70_002898"
     CDS             164..715
                     /locus_tag="IVY70_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HAP7959974.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
     gene            1109..1183
                     /locus_tag="IVY70_002899"
                     /pseudo
     CDS             1109..1183
                     /locus_tag="IVY70_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003725297.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transposition-like protein"
     gene            1183..>1334
                     /locus_tag="IVY70_002900"
     CDS             1183..>1334
                     /locus_tag="IVY70_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP7959975.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
BASE COUNT          493 a          187 c          258 g          396 t
ORIGIN      
        1 aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt attgggaatc
       61 ccagcttaaa tcatagatac cgtaagggat tttattcttt atttaaaact ttgcaacaga
      121 accgaagagc gagcaagaca ggaacgagag aacaaacata ctcatgaaaa gactagaggc
      181 ttcgacctcg aactttaata ataagttaga tgtttttgaa gaaaagacta agcatgcaag
      241 attaaacttt gaaacaactg caaagcacta tattaaacga cttgatgaag ataatttgaa
      301 attagatttt caacaagcta ttcaagatga attaagcgat actaaagacg aaatacgtga
      361 agtgacgaaa caagcgagag aagaaacaaa agagtataaa gaaatcttag aaagtaaaat
      421 aaaagaccat aacaaagtgg ttgataaatc gaatacagcg ctaaaagtga tgacaaaagg
      481 cgttacaaat atattttttg tgctaattat ttttgtactt gtaatgcttg tgacaggtcc
      541 gataggtcat ttctttggta ttgaacattt atatagcttt attaatggtt ttattgatga
      601 ccacgaaagt gcatggcgat atttgatgtt gatactttat gcagtaccct atgtattctt
      661 cgctatcatt ctgtggctca tagcattgtt ttttagagta tttaatgacc tttaggtaga
      721 tttatatata aaaagggcta aatacgcaaa ataccccctt aaaatgcaaa ataaatgcaa
      781 ataagggggt aactccttat gaaaaaacaa ctaatagtat tataacacgt ttttatgccg
      841 agaaaactta ttggttggaa tgggctatgt gttagctaac ttgttagcga gttggttgga
      901 cttgaattgg gattaatccc aagaaagtac caactcaaca acacataaag ccctgtaggt
      961 tccgaccaat aaggaaattg gaataaagca acgaaaggag ttgagtaaaa tgagtgagaa
     1021 aacgaagaaa atcattcgag tatcattagc tttttcacaa gtagtagtgc aatcattgat
     1081 agtttattat acttaccagc aatataaatg gttctgttgc aaagttttcc aaaaaatcta
     1141 ttttagtgta aaattgagaa aaaagacaga gaggacagag taatgaatca ttttaaaggc
     1201 aaacaattca aaaaagacgt cattattgtc gctgttggtt actacctgcg ttacaatcta
     1261 agctatcgtg aagttcagga attgttatat gatcgtggaa taaatgtttg tcatactacg
     1321 atttatcgtt gggt
//
LOCUS       DACCXO010000232         1326 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.240, whole genome shotgun sequence.
ACCESSION   DACCXO010000232 DACCXO010000000
VERSION     DACCXO010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.240"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            95..1264
                     /locus_tag="IVY70_002901"
     CDS             95..1264
                     /locus_tag="IVY70_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HAP7959976.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          486 a          197 c          226 g          417 t
ORIGIN      
        1 gagagtgtaa aatattttgt gtaaatagaa aaaaggaagt cccttctgta gaatagagtt
       61 accacaacac attcacagaa aagaggactt ccatatgaac gattttacta cagaaattat
      121 ggaaatacta atcaataaag gtgatttgga tgatttgttt cgtcgtcacc tagaattggc
      181 tattaacgct ttattacaag ctgaattaac ggtatttctt gactatgaaa agtatgatag
      241 agctggattt aattcaggta attcccgtaa tgggaattat tcgcgctctt ttaaaacaga
      301 gtacggtgaa ttgaatttag taatacctag agataggaat ggagactttt ctcaacaaac
      361 tttacctgct tataaaagaa ccaatgactc tttagaaacc actattattc agctagttca
      421 aaaaggaatt actatgtccg aaatctctga tctaatcgaa aaaatgtatg gtcattatta
      481 caaaccacaa actatttcaa atatgagtaa aattgtgtct gaagatgttt cggcatttaa
      541 agaaagaact ttagaagcta aatactcagt catttttatg gatgctactc atattccctt
      601 aaaaagacaa accgtatcaa aagaagctgt ctatatagta attggtatca ggttggatgg
      661 aactaaagag gttctaggat ttactatagc tccaactgaa tctgcttatg tttggaaaga
      721 gattcttcaa gatctaaaag accgtggttt agaagaggtt ttgttagtcg taacagatgg
      781 tttaagtggt attgacgata gtattcatag tgtttatcca aatgctcaat ttcaacaatg
      841 ttgtgtccat atatctagaa atatcgctca taaggttcgt gttagtgatc gacaagaaat
      901 ctgtagcgat ttcaaattag tttaccaagc aagttcaaaa gaagaagcaa ataatcaaat
      961 aaggtttatg atagataaat ggaaaaagca gtatccacga gtggttaaat tgctcatgaa
     1021 tcctgctata ttaacctttt ataatttccc tccgtcaatc agaagaacta tttattcaac
     1081 taatttgatt gaaggtttta acaaacaatt aaagaaatat accaaaagaa aagagcaatt
     1141 tcctaatgaa gaatctctgg agagatttct agtatctcaa ttcaacaact ataatcaaaa
     1201 gtttctatgc cgagttcata aaggttttaa agaaatacat gatacattag aatcaatgtt
     1261 ttaacttgat caacagaatg ggcttttcca tttacacata attattgaca caacctattc
     1321 tggaaa
//
LOCUS       DACCXO010000233         1320 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.241, whole genome shotgun sequence.
ACCESSION   DACCXO010000233 DACCXO010000000
VERSION     DACCXO010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.241"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            197..>1320
                     /locus_tag="IVY70_002902"
     CDS             197..>1320
                     /locus_tag="IVY70_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7959977.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGL"
BASE COUNT          506 a          210 c          280 g          324 t
ORIGIN      
        1 ccaaagctct tagttgaaac gtgggtcgat gtctattcga tgttgaccga cgttttttct
       61 atgccttcag atttggtttt aagcgatatc tgtttagatg acattttgca aatggcttac
      121 aacaagagtg cttatgaagg atggaaaaac tatgcaataa accaatccca gaaaaactaa
      181 agaaaggagg taaaaaatgg ctaaaaagag aacagaagca gaagtaacat tcatagctaa
      241 cgatgacgga ttgaaatcta cgttaaaaga aatcagtgct gaattaacta aaaatagagc
      301 agaattaaaa ctagaacaag ctcaattaca acagactggt tctgaatcag acaagttagg
      361 aagtaaatta tcttctttag aaaagcagta tgaattacaa agtcaaaaag ttgaagtaac
      421 tagccaacgt ttagccaatg ccaaaaaata ttatggagaa aattccaccg aagttcagaa
      481 acttgagaga gaactgatta accaacaaac agcgcaacaa cgtttgtcaa acgaaattga
      541 taaaacgagt aatgcactag ctcaagcaaa aggcgaaata cagacgtacg agtctacaat
      601 gcaacagttg gatagtgaac aaaaaaatgt tcaagctagt gcttctctga ttgaatccga
      661 atacaaaaaa tggcaagcaa ctgctggtca atcagcttct gaatccgaga aattagcgaa
      721 agcccaagaa tatgtttctc aacaatctga aaatgcagag aaaacgatag atatcctgag
      781 acgacagtta gaagctacac agtctgagtt tggcgctaca tccacagaag caatgcagat
      841 ggaggcgaag cttaatgatg ctgaacgtga atttgaagag ttaggacaag ctgctaaaaa
      901 tgtagataca actaacttgg acgatatcgg aagcaaaata gatatgaata atctaatgga
      961 agcttctgac gttttaagcg acattggcga taagcttaca gaattaggga aacaagcagt
     1021 ggactctgct aatagtgtag gtagttccca gagtaaaata caagctaatt ttggtttgac
     1081 taaacaagag gctgaagaat taacgaatgt agccagagac atttattata aaggttttgg
     1141 agaatcgtta gatcagtcca cagatgcatt gattttggta aagcgtaatt taggcgattt
     1201 aaataatcaa gatttacaaa atatcacgga acaagctatg gtcctagaaa acaccatggg
     1261 cgctgatatg gatgaaacgt tacgtggtgt aaatggctta atggtcaatt tcggcttgag
//
LOCUS       DACCXO010000234         1320 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.242, whole genome shotgun sequence.
ACCESSION   DACCXO010000234 DACCXO010000000
VERSION     DACCXO010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.242"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            197..>1320
                     /locus_tag="IVY70_002903"
     CDS             197..>1320
                     /locus_tag="IVY70_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7959978.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGL"
BASE COUNT          507 a          208 c          280 g          325 t
ORIGIN      
        1 ccaaagctct tagttgaaac gtgggtcgat gtctattcga tgttgaccga cgttttttct
       61 atgccttcag atttggtttt aagcgatatc tgtttagatg acattttgca aatggcttac
      121 aacaagagtg cttatgaagg atggaaaaac tatgcaataa accaatccca gaaaaactaa
      181 agaaaggagg taaaaaatgg ctaaaaagag aacagaagca gaagtaactt tcatagctaa
      241 cgatgacgga ttgaaatcta cgttaaaaga aatcagtgct gaattaacta aaaatagagc
      301 agaattaaaa ctagaacaag ctcaattaca acagactggt tctgaatcag acaagttagg
      361 aagtaaatta tcttctttag aaaagcagta tgaattacaa agtcaaaaag ttgaagtaac
      421 tagccaacgt ttagccaatg cgaaaaaata ttatggagaa aattccaccg aagttcagaa
      481 acttgaaaga gaactgatta atcaacaaac agcgcaacaa cgtttgtcaa acgaaattga
      541 taaaacgagt aatgcactag ctcaagcaaa aggcgaaata cagacgtacg agtctacaat
      601 gcaacagttg gatagcgaac aaaaaaatgt tcaagctagt gcttctctga ttgaatccga
      661 atacaaaaaa tggcaagcaa ctgctggtca atcagcttct gaagccgaga aattagcgaa
      721 agcccaagaa tatgtttctc aacaatctga aaatgcggag aaaacgatag atatcctaag
      781 acgacagtta gaagctacac agtctgaatt tggcgctaca tccacagaag caatgcagat
      841 ggaagcgaag cttaatgatg ctgaacgtga atttgaagag ttaggacaag ctgctaaaaa
      901 tgtagataca actaacttgg acgatatcgg aagcaaaata gatatgaata atttaatgga
      961 agcttctgac gttttaagcg acattggcga taagcttaca gaattaggga aacaagcagt
     1021 ggactctgct aacagtgtag gtagttccca gagtaagata caagctaatt ttggtttgtc
     1081 taaacaagag gctgaagaat taacgaatgt agccagagac atttattata aaggttttgg
     1141 agaatcgtta gatcagtcca cagatgcatt gattttggta aagcgtaatt taggagattt
     1201 aaataatcaa gatttacaaa atatcacgga acaagctatg gtcctagaaa acaccatggg
     1261 cgctgatatg gatgaaacgt tacgtggtgt aaatggctta atggtcaatt tcggcttgag
//
LOCUS       DACCXO010000235         1313 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.243, whole genome shotgun sequence.
ACCESSION   DACCXO010000235 DACCXO010000000
VERSION     DACCXO010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.243"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            200..742
                     /locus_tag="IVY70_002904"
     CDS             200..742
                     /locus_tag="IVY70_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959979.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1272
                     /locus_tag="IVY70_002905"
     CDS             898..1272
                     /locus_tag="IVY70_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP7959980.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDVEWQHFF"
BASE COUNT          532 a          148 c          256 g          377 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgt ggaatggcaa
     1261 cacttttttt gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gct
//
LOCUS       DACCXO010000236         1308 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.244, whole genome shotgun sequence.
ACCESSION   DACCXO010000236 DACCXO010000000
VERSION     DACCXO010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.244"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(76..1170)
                     /locus_tag="IVY70_002906"
     CDS             complement(76..1170)
                     /locus_tag="IVY70_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP7959981.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT          387 a          276 c          200 g          445 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt
     1261 ttaaaataaa tgataaattc caataattta agtaggtatt ttccctct
//
LOCUS       DACCXO010000237         1294 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.245, whole genome shotgun sequence.
ACCESSION   DACCXO010000237 DACCXO010000000
VERSION     DACCXO010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.245"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..1244
                     /locus_tag="IVY70_002907"
     CDS             <1..1244
                     /locus_tag="IVY70_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP7959982.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DACCXO010000238         1289 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.246, whole genome shotgun sequence.
ACCESSION   DACCXO010000238 DACCXO010000000
VERSION     DACCXO010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.246"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(15..1154)
                     /locus_tag="IVY70_002908"
     CDS             complement(15..1154)
                     /locus_tag="IVY70_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP7959983.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT          336 a          281 c          239 g          433 t
ORIGIN      
        1 ttattaaaaa aatcttacgc taagtaggat aaaataattc cttgatattt ttcgatcata
       61 tccaaataat tatcgatttc aacatactca tctacttgat gaggcaaagt aacgacacca
      121 ggcccaaaga cgacaaagtc gaaggaatgg ttagctttcg taaattcggc tgcatccgtt
      181 gttgctgccg ctccaactaa aggtaatggc tggctgaatt gttgctgaat gcaatgaatc
      241 aaaggagaat ctggatcagc tttgacaggt attttgttgt aatcgatcat caattccaaa
      301 tgatagtctg tctcttgatt caattcattg acgattgatt gcagcaatgc aatgatttta
      361 tcatttgggt attctggaat cgatcgaatg ttcccttgga gctgcgcatg gcttgggatg
      421 ctgttgactt gattcccgcc actgatcaat gtaacattat ggattgtttt tcctaggaca
      481 ggattctcga ttgtttcagc gaggtgattc atttcagcat ttgctttcgt aatgaattca
      541 tttaggtgat tgattgcatt ataaccttgg tctggcatag agctatgcgc ttcttttccg
      601 tgcgaggtga ctgtatagtt gattgatccc atgtgcgtat acatcaaact gtaattggtt
      661 ggctctccga tgatcaaggc atctaagtca tctacatagc ctgctttcgt gagctgttcc
      721 ccacctagtt cacccacttc ttctccgaca gtggctaata atttgactgt tccgttaaat
      781 ggttttcctg actctttcag ttcgatcatg gcaatcacca ttgctgccaa gccactcttc
      841 atatctgtag caccacgtcc gtacaaacgg ttcccatgga tttctgctgc aaagggtgca
      901 taggtccaag acgattcatc accagccgcc acgacatcca tatgcccaga taggcctaac
      961 acttttcctg attggccctt ttggtaacga gcaatcaaat tatctctacc atctctgtaa
     1021 gaaacaatct ctcctgttat gtcatgcttt gccaaaagct tattcaaata ggctgctact
     1081 tcaccttcgt ttccattcac tgatttaatt cggataatct cttgtaaaat cgcgattttt
     1141 tcttcttttt tcatctaatc ctctcccttg ccatttcttt ttattgtatt tgagtgtgct
     1201 cgaattttca aataggagca accaaagtaa aaatgaagaa agagagccac tttatgaaaa
     1261 atatgctgct acttccctcc tattttatt
//
LOCUS       DACCXO010000239         1287 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.247, whole genome shotgun sequence.
ACCESSION   DACCXO010000239 DACCXO010000000
VERSION     DACCXO010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.247"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(88..1128)
                     /locus_tag="IVY70_002909"
     CDS             complement(88..1128)
                     /locus_tag="IVY70_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP7959984.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFIPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAETESGISIIFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SFPKTITKDWLE"
BASE COUNT          383 a          204 c          181 g          519 t
ORIGIN      
        1 gtaacatttg taatataatt agaataataa aaaattttta attttaatgc tactccatga
       61 agcaacaatc aatttgtcag tgaaatttca ctctaaccag tctttcgtaa tagtttttgg
      121 gaagctttca gaattcaaac gtttttcact tgatacttta gcagctattg gtttttcgat
      181 aaaccaattt tttacataga caaaaatcag gttctggata ttttcaactt tttcggtttt
      241 taatttttga taggctttta acagtgtcgt gtataattct tgttctgcat tgacataata
      301 actatcgagt tcttcaaaaa ttattgaaat accactttct gtttcggctt ttttctttgc
      361 attatgaata gtctggacag ttttttgagc ttcactgtaa cttgaagaaa atgtttttat
      421 taaatttaat actcgttcag ggataaacgt agcaatgctg tcgtttgtca taattgtctc
      481 aaaattgtct aacaacaatt gatcttgtag gcggtctttt tcagtgtcta tattgtgtct
      541 attagtgtct aatatattat tattaagttc tggtatgatt ttcataccct ctgatttatc
      601 aacgttttgg cacggtatga ttttcatacc ctcttgagcc ccaagggatt cagcgtctga
      661 tttttcgtcg agggtgggta tgattttcat accctctgat ttatcaacgt tttccctatt
      721 ctctctatca aatttttcga gtaagtagat atcattttct gagactttta attctgctag
      781 atatagtcta ttaggattat ttttccctaa ctgtttgtta aatcccatct gcttttgtaa
      841 taataaacca aaggattcca actctttttt gattgctatc gctttttgtt tttggcagtt
      901 taatacgcta catagttcat ttgtcgtata agtaaaataa acattacctt cttcatcgat
      961 ttgccgtttg ctgattgcga tctctaatct tgcttttaaa agcatatagg caatcttagt
     1021 tgaatctttc atttttttgt attcgtcaga aacaataaag acttttggaa attgataaaa
     1081 taattcgttg taaactctat ccgccttaaa aaaattaaaa tcagtcattg tagatcctct
     1141 actttcacgt tgttttattt atgtaaaatt acctactctt ttcgaaaata gcaaagataa
     1201 ataaaaaatg caaacgcatt aaaactgttt tttaattaaa tactacctaa aatcagaaat
     1261 aaaatgaatt atgtactacc tttaaag
//
LOCUS       DACCXO010000240         1248 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.248, whole genome shotgun sequence.
ACCESSION   DACCXO010000240 DACCXO010000000
VERSION     DACCXO010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.248"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            75..1160
                     /gene="xerS"
                     /locus_tag="IVY70_002910"
     CDS             75..1160
                     /gene="xerS"
                     /locus_tag="IVY70_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP7959985.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          499 a          211 c          190 g          348 t
ORIGIN      
        1 caaaacactc tataattggt atagtccaat tatagagata ctcctctccc ctacttatcg
       61 aaaggatgaa taacatgaaa aatacagaaa tatatcaaaa aatagataca atcatacttc
      121 atatgcccga ttatataaaa gattttgttc aagatcgtga ggataaagat caatcacctc
      181 gaactacttt agaatatcta aaaaattata agctctttta tgaatggctt ctgagtgagt
      241 ctatcgttcc cgatacttca atcacctcga taagacagtt aactgcctat gatctaaact
      301 tgtacaaatc tcatttgaaa cgtcgcacaa aggaaaatgt aaaaaaagag acaacaaaaa
      361 ctaagctcaa agataataat aacttaggtc tatctacttc tactatcaat agaaatatca
      421 cagcattaaa ggttctattt aaatatcttt cgaaaagttc caataatcct ttaggaaaac
      481 catacttaga aaataatcct atggatcaag tagcaactat cacagataaa actacgttag
      541 ctgcccgtgc aaacgcaatc gagaaaaaat tatttctaga cgaagataca caaaattatc
      601 tagattatat tgccaatgaa tataaaaaaa ctttgagcaa acgagctttg atctattatc
      661 aacgagatgt agaaagagac ctggcgatca atgcattgat tttaggatct ggattaagac
      721 tctccgaggt tgtaaatatt aatttagagg acctatcttt agaaaagaat aatgtggtag
      781 taactagaaa aggaaataag agagacgcag taaatatagc agcatttgca atggaatatt
      841 tagctaatta tctagctatt cggaaagaac gatacaaagt aacagaaaac gaaaaagctt
      901 tatttctagc aatttatcaa ggagaagcca agcgtatatc cggtattgca atcgaacgga
      961 tggtagcaaa atattcaaaa ggattcagag tccaagtcag tccacataaa ttacgtcata
     1021 ctttggctac tagactctat caacagacaa attcattggt attaactgcc caacaattgg
     1081 gacatacaag tacaaataca acaactttat atactcatat agacaatgca gctacaattg
     1141 atgctttgaa cgctttgtaa atgtagtaaa aaatagtata ataatgaagc ctgtctctaa
     1201 aattagataa aagcccaact ttaaaggcaa gcttcattat cattttta
//
LOCUS       DACCXO010000241         1237 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.249, whole genome shotgun sequence.
ACCESSION   DACCXO010000241 DACCXO010000000
VERSION     DACCXO010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.249"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            38..991
                     /locus_tag="IVY70_002911"
     CDS             38..991
                     /locus_tag="IVY70_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7959986.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            963..>1237
                     /locus_tag="IVY70_002912"
     CDS             963..>1237
                     /locus_tag="IVY70_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP7959987.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
BASE COUNT          447 a          190 c          235 g          365 t
ORIGIN      
        1 tactaaatat atttagtata ggaaattgga gggaactatg gaaaacttac ggactgcatt
       61 taaaacatca gataaactag gaaaagttgt aatgcttaca ggtagaaaag gcggcattgg
      121 taagactaca gacaatgatt tattagcgat tgtttctagt caactttttg aaaaagacgt
      181 gttgctaatt gattatgacc aacaaagaaa tactacttcc aacattggat caacgtatca
      241 aattactagc tttgatcgat ccatgtctgc agcaatcaaa aaaggggact gggtttcagg
      301 tattactcaa gtaagtccac atttatatat tatggctggc tctcctggat ctgaagaact
      361 taatgaatac ttgtccgaaa aatatcctga tcgtcgtaag agatcattgg ccttcattaa
      421 acctttagaa gaattgagaa aaaatttcga ttatatcttt atcgactgcc caccatcaac
      481 agataatgtg gttcgtgcat tcttaactgc tgcagattat attattgcaa tgcaagaatt
      541 aaaacggtac gcaatggaag gaacagaaga ttttattaat aaagttttag ttccgattgt
      601 cacgaatttt gaagagtctc atcttcaaat cattggaatt ttgcctgttc ttttctcagt
      661 gcgacgttcc tctcaacacg caaactatca aaaaacaatt gataagtatg gtgaatcaaa
      721 catttttaaa agcattgtta aaggctcgga tcggttagaa atgtatggtg agaatggtat
      781 ccaattaaat gattatgtag atcgaagatg gtgggctatt tttgcagaca tctttacaga
      841 attagaagaa agaattgaat actatgaaca acatggtgat attgaaggat ttgattatac
      901 acctaaatat gctgactcaa tggccaatca tatcctacct cttggaaagg agattaaact
      961 aaatggcatt attaccaacg aacaaagata agaagaaaat tggtgctgaa gatatttcaa
     1021 ttaaagatga tattaatgtc gagttagggg aacgaaagta cactgcgaaa gaaagaaaac
     1081 cagttcaagt agatcctccc gtactaaaaa ccattcggga tcttagttat gcaaaagata
     1141 ttcctatgta tgaaatagtc aaacttgcaa tggacgccta tgttgatact ttaaacgacg
     1201 aggaacggat tatatataat agacgatcaa aagatta
//
LOCUS       DACCXO010000242         1230 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.250, whole genome shotgun sequence.
ACCESSION   DACCXO010000242 DACCXO010000000
VERSION     DACCXO010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.250"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(47..970)
                     /locus_tag="IVY70_002913"
     CDS             complement(47..970)
                     /locus_tag="IVY70_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959988.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
BASE COUNT          392 a          221 c          125 g          492 t
ORIGIN      
        1 ggaaatcaca acttttttct tttacgataa atcaatcctt attaaattat ttaggaaaat
       61 attgttcaat tatttcttta tatattttct catagtatgt ttctgtccat cctaatgttc
      121 ctataaaatc aagtcctcta cattttaaag gttcacttaa actattaata tagttaataa
      181 tgttgtctat ccttttctct ttatcttcac cttcaatctg gggagcatat agtccaattt
      241 cctcccaata atatgaatct ctttcaattc ccatgatggt agctttggct aatttcaaat
      301 caaataacga ttttagaatt cttagtgtct caataaagtc ttctctcttc aactctttaa
      361 tccattgtga attttctgga ttatctaaaa tttcaagttc atcttcatta taagtatatt
      421 tcaaatttga cttgcttaac tcatagataa atgaagtttc ttgactcata attcttttaa
      481 gtacttgact ataagtttcg tttgactttg cccatttcgc taattctacc actgtattca
      541 ctaataaatc gaaataatga tgttctcgtt caatagtctc agcattttga aaatgaaaat
      601 cacctttata aactccattc ttttccactg ttttattaat attttttaaa aaccagaatg
      661 agtttgcaaa tttagtcgta ctttttcgat taactaagtt aataactctt ccatgtaata
      721 ataagacaaa aatacataaa tttttagctt ccttattatt agatactaat tgatcaatca
      781 tagggagcca ttttttgatt ttcactaatt gattgctcat aaatctttca ctggactttt
      841 gttcaatctt aaattgcaaa acttctaact cataattccc atcttctgat ttatttttgg
      901 gaacaactat caaactttta taaagtttat ccacatcaaa atttatcttt tttgcataat
      961 ttgtagccat taatacatct ccttattcat aataaatttc atatgacgct cctctcaaaa
     1021 tttaccggta gactagaaaa aagaataaaa gatacgtcaa ttcacacttt attcttaaaa
     1081 aaaaactggt ctctctaccg gtaatatcga ttgagtccgc ctttatcgac tttatccttt
     1141 ttttatatta aattttttca ccggtagaca gggagagaaa gaataaacta aaaagccctt
     1201 aattcttaat gttttcacct ccttaccgtt
//
LOCUS       DACCXO010000243         1213 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.251, whole genome shotgun sequence.
ACCESSION   DACCXO010000243 DACCXO010000000
VERSION     DACCXO010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.251"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..488)
                     /locus_tag="IVY70_002914"
     CDS             complement(<1..488)
                     /locus_tag="IVY70_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959989.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGG"
     gene            complement(498..>1213)
                     /locus_tag="IVY70_002915"
     CDS             complement(498..>1213)
                     /locus_tag="IVY70_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP7959990.1"
                     /translation="VINMKQFKLSGYIPVCKAMLDLGPVWLDRYVRTVLVESLRIALE
                     QAIVDGTGKDMPVGMMRDMSKQTSGEYAEKKAEPITALDAVTMGGLMARLSKFNIEGV
                     NDPIYRNVNPSDVVLIVNPTDYWSKVFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEG
                     KAVIGVASDYFMGVGSTLKIEASDEYHFVEDERIYLAKQYANGQPKRNDSFIVLDISA
                     LGTTTTTTKPTTTTTTTQA"
BASE COUNT          363 a          257 c          190 g          403 t
ORIGIN      
        1 cctcctgaat tagtgtttat agtttccaaa gattctactg taacatttac aatgtttgcc
       61 atatctttta gaatattttc tccgttttca cagcttctaa tataatcaag acttagataa
      121 ctacagcagt cgctacaata ccaaacatca tctttagaag gcaatttgcg caaattaatt
      181 cttttattga aaatgacatc cgaatcgata tagaaatatc ggtcgtcctc acgcgaatga
      241 tcttcttcta aatatttcca ccataaatat ggtttaatcg aaggaatata ctctttgtcg
      301 tcccgcagat catcgtacac atgaacttca acaccatatt ccttctcaaa aaaaatagga
      361 atctgatcat cgtgtctgct gaaaagcaat acgatatctt tgattcctag tttcttcaga
      421 ttagttaaac aaacttcaag ctcccattta aaccgattga ttgccggctg acaaagaata
      481 tacttcattc tgatcaccta cgcttgtgtt gtagttgttg tggttgttgg ttttgtagtt
      541 gtagtagtag ttcccaaagc gctaatatct aatacaatga aactatcgtt acgtttaggt
      601 tgaccgtttg catattgttt agctagataa atgcgttcgt cttcaacaaa atggtattca
      661 tctgaagctt caatttttag tgtagatcct acacccatga agtaatctga ggctacccca
      721 ataactgctt ttccttctgg cacagccgtt gactgcaaat ctgaaactgg tactggcaat
      781 acttgtacgt attctccatt agcagttagt acagtcttag ctgggaatac tttagaccag
      841 taatcagttg gattcacaat taggaccaca tcagaaggat tcacattacg ataaatcgga
      901 tcattcacac cttcgatatt gaattttgat agtcgcgcca tcaaaccgcc catagttaca
      961 gcatctaaag ctgtaatagg ttctgctttt ttttcagcat attctccgct agtttgtttg
     1021 ctcatgtcac gcatcattcc gactggcata tctttaccag taccatcaac aattgcttgt
     1081 tctaatgcaa ttctcaatga ttctactaaa acagtacgga cataacgatc taaccatact
     1141 ggacctaaat caagcattgc cttacataca ggaatataac ctgatagctt gaactgcttc
     1201 atgttaatta cat
//
LOCUS       DACCXO010000244         1205 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.252, whole genome shotgun sequence.
ACCESSION   DACCXO010000244 DACCXO010000000
VERSION     DACCXO010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.252"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            65..340
                     /locus_tag="IVY70_002916"
     CDS             65..340
                     /locus_tag="IVY70_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7959991.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            767..>1205
                     /locus_tag="IVY70_002917"
     CDS             767..>1205
                     /locus_tag="IVY70_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP7959992.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEF"
BASE COUNT          452 a          205 c          182 g          366 t
ORIGIN      
        1 ttgttttcat ataaccaaag gcgctatgat ttccatatga aaaaaataaa gtcaggtgaa
       61 aaaaatgaaa cttgaggata aaaaagtcat tttgaagaaa atcaaacgac taccagacca
      121 aatagatggc tatacgatct gttctttcaa aaaagacagg agtataaaag cttatacgca
      181 aaacacagaa aaaggaataa tctatttcct acatgaagaa ggttacgata cccaaacctt
      241 tatgtttact gaaaaaaaag aatttcataa acagatgaaa aaactgattg aaaaagaatt
      301 tccaagaagt cataaactct atgtgaatca gcaattttaa cttctaactg ttctttatta
      361 attaaaaatg ttctgtaata aaataaaact ttataacaac tataacaaaa ccgaatgcaa
      421 aacagaagtt ctataagtta taatcatagt tgttaggtga ttcatctagt aaaaacacca
      481 gatttcaaat ttcacacaat ttaaactaga tcaatcatca accttcttca aattgttaga
      541 accctttccc gaggagctgc agcataagca aaaaaacaaa caggaagcca aatggctaag
      601 acattttttc tattagtcta gttgtttctc gtcgcaagtt taagacttta gaaagttatt
      661 tctaatgatg tttgggttgt tttgatcaat gcttatgcac agctccttac ctattattta
      721 tctcaacaga tctaacaaat taatggacta tttaaggagt ttttttatgc ttacgatcta
      781 ttcttctcgc acacatagtt accatctgat ggcaaactgg ctgaaagacc atcaaatgcc
      841 ttacagagaa cgaattttca cacctaaaca gcctttaaca aggaaagaga tattttattt
      901 attgtcatta acagaaaacg gatttgacga tctattagct aaacgctcga ttgattataa
      961 aaatttgaaa gaccaaatcg accaatgtac gacctcagag cttgtcgatc tgatcgtctc
     1021 gcattttact ttgttacaac gtcccatcat tactgacgga aagaaattat tagtcggatt
     1081 caatgaagat agcatgcgtc tttttatccc tgtacaatgg cgcagaagtc aactgaaatc
     1141 tgtctataac gattttacat gggtaagcaa tctagaaaat tctacggatt tagatgatga
     1201 atttt
//
LOCUS       DACCXO010000245         1158 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.253, whole genome shotgun sequence.
ACCESSION   DACCXO010000245 DACCXO010000000
VERSION     DACCXO010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.253"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            71..757
                     /locus_tag="IVY70_002918"
     CDS             71..757
                     /locus_tag="IVY70_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP7959993.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            757..>1158
                     /locus_tag="IVY70_002919"
     CDS             757..>1158
                     /locus_tag="IVY70_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP7959994.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWG"
BASE COUNT          440 a          164 c          253 g          301 t
ORIGIN      
        1 gataaggcga tggcagatga aatatttata actaaaaaca atcaacaagt atcgaaagga
       61 ggtgaggcga atgacgacaa tgaaaacatt tctagcagta aagaatgaag gcgcagtacc
      121 gcaaattttt attcagggat ttattggttc tagttggttc tttgaaggga atactgacaa
      181 gggaatcaaa aatattttgg atagtctagg tgatcaagaa gaaattgaag tagtaattaa
      241 ttcaaacggt ggagacgtat ttcaagggat tgctattggg aacttactta agtcaaataa
      301 agcaaaagtt aacgttgtga ttaacggctt agccgctagt gctgcttcaa ttatcgcaat
      361 ggctggcgat actataaaaa tctacaacaa tgcacaattg atgattcacc gcgcttccac
      421 atacggagaa ggaaatgtcg atgacttccg tacgattgct gaccaactgg aatcaattga
      481 taaatcggta aaggcttcat ataaaacacg attcaatggc acagatgaag cattgcaaga
      541 acttcttgaa aaagaatcgt ttatggatgc agaaacagct ttgagttatg gattggtcga
      601 tgaaattatc gatgcagaaa atagctcagg tactgaagct aaaaaagaac aaagcgttga
      661 agaaattttg aatgacgtta aagaaaaaag agcagaaaaa attgctgcat ttacagcagc
      721 attaaataaa acatttggac aaggagatgt aaaataatga cagttaaaaa tttaaaaggt
      781 gtaacagctg caagcgacca attgatgaaa gcttttaaag atggtaacga agaatctttt
      841 agcgcagcta tggtaagctt atctaaggaa attcaggata aaattttaga agaagcaaca
      901 gcaaaaaatc aagatcaatt agtattaatg aaccgtggtc agcgtgtatt aactacacaa
      961 gaaacaaaat tctataacga agtagtgaaa aacgaaggtt ttgcaggggt cgaagaatta
     1021 gtgccagcta ctgtatttga acgtgtattt gaagatttag aacaatctca tccactattg
     1081 caaaaaatta cttttgttaa cacaactggt gtaacagaat ggattgtgtc acgtggagtc
     1141 aatccagcat ggtggggt
//
LOCUS       DACCXO010000246         1149 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.254, whole genome shotgun sequence.
ACCESSION   DACCXO010000246 DACCXO010000000
VERSION     DACCXO010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.254"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            34..906
                     /locus_tag="IVY70_002920"
     CDS             34..906
                     /locus_tag="IVY70_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7959995.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          334 a          220 c          278 g          317 t
ORIGIN      
        1 tgatttcatt ctaaaataaa gggagaaatg tctatgctat acggtgcaat tgaagcaggc
       61 ggcacaaaat ttgtgtgtgc cattggcgat gaagaaatga cgatcaagga aagagtgagc
      121 tttccgacaa cgacacctga ggaaacaatg cctttagtca ttgacttctt caaacaatac
      181 caagcagatt tagcaggaat tgggattggc tcatttgggc ctatcgatat ccatcgcgat
      241 tccgccactt atgggtacat tacctcgacc ccgaaattgg catggcaaaa ttttgatttc
      301 atcggtacca tgaaaaaaga atttccgatc ccgatttctt ggacaacaga tgtcaatgcg
      361 gctgcctatg gtgagtatgt cttcggcagt ggcaaaggat tgagcagtgt cgtttactat
      421 acgatcggta cgggtgttgg cggcggtgcc ttacaagaag ggcgctttat cgaagggttt
      481 agtcatccag aaatggggca tatgctcgtc gtccctcatc caaaagatga ctttgctggc
      541 agctgtccat ttcatggcaa ctgtttagaa gggatggccg ctggacccgc catcgaaaac
      601 cgcttaggga aaaaaggaca agaggttgca gaagatgatc cttattggga aatcgaagcc
      661 tcctatattg ctcaatgtgc ctacaataca accttgatgt tttctccaga tgtgatcatt
      721 tttggcggtg gtgtgatgaa acaacgccat atgctgcaaa acgtccacga cgcatttgca
      781 aaattggtca atgggtatgt cgaaacacct gagttaagca aatatatcgt gaccccagca
      841 ttagaagaca acgctggaac gctcggttgt ttggcattgg ctcgagaagc ggtcttacat
      901 tcataaacga aaaaaggctc ttcgacattt agtgttggta gagaaagata caactgaaaa
      961 tttagtcata accagttagg cagagtgctt aactggtttt tgttttactt ggaagatcaa
     1021 gccttgaatg agataaattc ttgctcgaaa gttatggaaa tttcgatagc cgtaggccac
     1081 ttgtattgtg caatagtttt ttacaaaaaa aagggggtgc ggatattttt ttggaccttt
     1141 ttaacaaat
//
LOCUS       DACCXO010000247         1105 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.255, whole genome shotgun sequence.
ACCESSION   DACCXO010000247 DACCXO010000000
VERSION     DACCXO010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.255"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            32..>1105
                     /locus_tag="IVY70_002921"
     CDS             32..>1105
                     /locus_tag="IVY70_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7959996.1"
                     /translation="MGGASIVASKIAKGLIDSGHEVFFVSYLESEKPQVESKYYDLYE
                     KKIFLFKNFSRFHKILEYRLFGGFVPSRYIKKEKEQLKKVLKKEKPDVIIFNTFIPSV
                     LFSEFLKYEFPTVKLITWMHSDPDYSLNHIAKFYKKEYRKSFERVDSIVCLSQDTKEK
                     LSLYNNHIKVIYNPLTLSGSSLISNLDKKIISFTARYDINIKGFDYLCEVAKNLPVGW
                     AIRIAGDGTEEERKELKRLIEKNGVLDKIICAGALFGDELINHYIESSIFISTSRSEG
                     LPLVLIEAMTYGLPVISFDHGGAKEILQDGNYGILIKNFDVQDMNNEIEKLSNNRLLM
                     EKYQKFSLERAKDFNEKKIIGEWQ"
BASE COUNT          428 a          125 c          189 g          363 t
ORIGIN      
        1 gaaaatagct atactatctc cagctttctc aatgggagga gctagtatcg tggcctcaaa
       61 aattgcaaaa ggattaatag atagtggcca tgaagttttt tttgtttctt acttagaaag
      121 tgaaaaacca caagtagaat ctaagtatta tgatttatac gaaaaaaaaa tttttttatt
      181 taaaaatttt tctcgatttc ataagatatt agagtataga ttgtttggag gctttgttcc
      241 tagtagatat ataaaaaaag aaaaagaaca actaaaaaaa gttctaaaga aagaaaaacc
      301 tgatgtcatc atattcaata cttttatacc tagtgtacta ttctccgaat tcctaaagta
      361 tgaatttcca acagtgaaat taattacttg gatgcactct gatcctgatt acagcttaaa
      421 tcatattgct aaattttata aaaaagaata tagaaaaagt tttgaacgcg tggattccat
      481 tgtttgtctt tcgcaagata caaaagaaaa actttcatta tacaataacc atattaaagt
      541 tatttataac ccattgacat taagcggctc tagtttaata agtaaccttg ataaaaaaat
      601 tatttcattt actgccagat acgatataaa tattaaaggg tttgattatt tatgtgaagt
      661 agctaaaaac ttacctgttg gatgggctat aaggattgct ggagatggta cagaagaaga
      721 acgtaaagag ctgaaaagat taattgagaa aaatggtgtg ttagataaaa ttatttgcgc
      781 aggtgcatta tttggagatg aattaatcaa tcattatata gaaagttcta ttttcatatc
      841 tacttctaga agtgagggac taccattagt gttgatagaa gcaatgactt atggattacc
      901 tgtaataagt tttgatcatg gtggtgctaa ggaaatatta caagatggaa attatggaat
      961 tttaattaag aattttgacg tacaagatat gaataatgag atagaaaaat tgtctaataa
     1021 tcgtttactt atggaaaaat atcaaaaatt ctctttagaa agagctaagg attttaatga
     1081 aaaaaaaata attggtgaat ggcaa
//
LOCUS       DACCXO010000248         1097 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.256, whole genome shotgun sequence.
ACCESSION   DACCXO010000248 DACCXO010000000
VERSION     DACCXO010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.256"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            57..905
                     /locus_tag="IVY70_002922"
     CDS             57..905
                     /locus_tag="IVY70_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7959997.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
BASE COUNT          381 a          194 c          191 g          331 t
ORIGIN      
        1 taacattaat gagaacggaa aaaatgataa tcttattatg aaaatgagga attctcatga
       61 gcgtctccat aagactacaa atcaaaacaa tgtacaacga cttcagccca aaagaacagg
      121 ctatcgccga ttatatttta gaaaatccaa gcaaagtaag tcatagtcct attagcaatt
      181 tagcaagtga actgggcatt gctgactcta ctttttttca atttacaaaa aaacttggtt
      241 ataatggatt taaagatttt aaaatggcca tgttgatcca agaaaatgat ctatctgcta
      301 tttctatcca cgaaaatatt cagaaaacag atactgagct cactatggcg caaaaggtgt
      361 tcgagtctag tatttcaact ttaaatgaca caaaaaagtt gttacaacaa aaggatctaa
      421 aaaaagcggc tactattatc aatgaatcta aacgtgtcta ttttttcggt gttggcggtt
      481 cagagattgt agcaactgac gcttatcata aatttttgcg ttcccctatc gcaacttctc
      541 acagcacgga ttaccatatt caattgatgg aagcttcttt attgacggaa aaagactgtg
      601 ctgttttgat ttctcacaca gggcaatcaa aagaaacaat ccatattgct gaaaccgtaa
      661 gaaaaactgg agcaaaaaca atcgttatta ctagccaagc taattcttcc cttgctaaac
      721 ttggcgatgt cgtttttatt tctatttctg aagaaacaga attccgatct gaagctttag
      781 cttcccgtat ttctcaacta agcattttgg actctctata tgtgattttg atgttttaca
      841 acgaaaaaga atcacgtcgt tctatcgcta aagttcgaca tactatttct gaaatcaaag
      901 aatagcacca taacagaaaa agcgaaaaat ccataaaaca aaaaaagcca ctaccaaata
      961 tttggtagtg gcttttttgg ttctgcaagg gtggtacaga acatcaatag tcggtgggtt
     1021 agaacgtatg tcatcgcatc tttggagaag gttgtattgg tattattcga gttacttgtt
     1081 agatgcgata acaaagt
//
LOCUS       DACCXO010000249         1091 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.257, whole genome shotgun sequence.
ACCESSION   DACCXO010000249 DACCXO010000000
VERSION     DACCXO010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.257"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>1091
                     /locus_tag="IVY70_002923"
     CDS             <1..>1091
                     /locus_tag="IVY70_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7959998.1"
                     /translation="SLAPIGNTIIDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGL
                     TAAFTLLMPIIVGFMALGGPTTLIIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTD
                     WLGGTWESIKEGASSVWDGVKETWSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQ
                     GVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFD
                     VAGQLLGAAWQAVWTPISDFLKNTWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIW
                     TAISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSI
                     SSTASNIWNSVTSSISNAINAAKSAIQSV"
BASE COUNT          327 a          167 c          259 g          338 t
ORIGIN      
        1 tcattagctc ctataggcaa cacaatcata gatgcactcg aaccagtaat tgattttcta
       61 ggaaagatgg ctgatgcgtt taataatctt ccacaaccag tacaggatta tgccgtagcg
      121 attggcggat tgactgctgc atttacttta ttaatgccaa taatagttgg cttcatggct
      181 ctaggtggtc ctactacatt aataatagga gcagttatta ctgttattgc tggagttata
      241 gcaattataa aaaactgggg cgcaattact gactggttta agggaatatg gagtaaattc
      301 actgattggt tgggtggtac ttgggaaagt ataaaagaag gtgcctcatc agtttgggat
      361 ggagttaaag aaacctggtc tggatttgta gattgggttc aagatatttg gcaaggagtt
      421 tctgattggt ttggagagct atggagcgga ttagttgaag gagcttccaa catctggcaa
      481 ggagtccaag agacttggca agcattcgtt gattgggttt caaatatttg gaacggagtc
      541 aaagaagtat ggtcgattat ttgggcagac attgtaggaa ttgttcaaat accatggacc
      601 ttaataacgt cattgattca agccggtatt aatattatcg tgggtatttt tgatgtagct
      661 ggacagttat taggcgcagc ttggcaagct gtttggacac ctatttctga tttccttaaa
      721 aacacttggg atactatgac acaatgggta agcatcgctt ggaatggaat tgtaactaca
      781 ttccatacta tatttgatcc agtagtggca tggtggaatg gtatatggac agctattagt
      841 actacggctt caaatatttg gaattcaatt agtgcaacag cttctagtat ttggaacagt
      901 atcaagaata caatcactag cttggtacaa gcagctgcta cagtaattca aaatatttgg
      961 tcaactgtat ctagttggtt aggtggaatt tggaattcaa tcagctctac agcatcaaat
     1021 atctggaata gcgtgactag tagtataagc aatgctataa acgcagctaa aagtgccatt
     1081 caaagtgttt g
//
LOCUS       DACCXO010000250         1073 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.258, whole genome shotgun sequence.
ACCESSION   DACCXO010000250 DACCXO010000000
VERSION     DACCXO010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.258"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..1057
                     /locus_tag="IVY70_002924"
     CDS             <1..1057
                     /locus_tag="IVY70_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7959999.1"
                     /translation="GTRFSQEYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEI
                     FKALDDSTIKKVGIIGAGYIGVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVE
                     EILSKNGIHLCLSESPKKYLGRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKEL
                     LRDVSGAYLTNEYQQTNIKDIYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHI
                     NNQDFTPSGSQFTNGFSIFNYNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNS
                     RVKLRISFEKNTKRIVGVQLCSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFS
                     QPYNFILKAVTGLNIET"
BASE COUNT          408 a          134 c          181 g          350 t
ORIGIN      
        1 tggtacaaga ttctctcaag aatatgacaa agttatatta gctacaggtt caaagcaaat
       61 tatgctagat ataccgggga ataatttagc taatattttt tcgataaaga attttccaaa
      121 tgcaaaagag atttttaaag ctttggatga tagtactatt aaaaaagtag gaattattgg
      181 agcaggatat attggagttg aaattgctga agccataaga aaaagaggga aagaagtata
      241 tcttttcgat gttgcagata gagtgttgtc gacttattac gaccgtagtt tttcagataa
      301 agttgaagaa atactatcaa aaaatggaat acatctttgt ctatcagaaa gtcctaaaaa
      361 atatttagga agaaaaaaga ttgaaaaaat agtcactaca agtggtaccc aatactctat
      421 ggatatggtc gtacaagcta taggatttct atctaatagt ccaattgggg aaaaggaact
      481 cttgagagat gttagtggag catatctaac taatgaatac cagcaaacaa atataaaaga
      541 tatatatgct attggggatt gtgctacaac tttttttacg tccattaata gaagtagtgt
      601 tgatttttct atttctaatg ctctaagaac agcttatatt gcaactcaac atattaacaa
      661 tcaagatttt acaccttctg gttcccaatt tacaaatggc ttttctatat ttaattacaa
      721 tttttacgca gcaggtttga atttaaaaac agcacgatta ttaagtgagt ctatagatta
      781 tatagaaata gaagatttca ttagacctgc ttttttgaga aaaaatagtc gtgtaaaatt
      841 aagaattagc tttgaaaaaa atacaaagag aatagtaggt gtacaacttt gtagtcaaga
      901 agatttatct ggactattat caatgttttc tcttgctata gaagaacaaa ttacattaga
      961 caaattgaaa atgttagatt atttatttca tcctagtttt agccaaccct ataattttat
     1021 tttaaaggca gtaacaggat taaacattga aacataatcg ttactaaatg aaa
//
LOCUS       DACCXO010000251         1064 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.259, whole genome shotgun sequence.
ACCESSION   DACCXO010000251 DACCXO010000000
VERSION     DACCXO010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.259"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(39..995)
                     /locus_tag="IVY70_002925"
     CDS             complement(39..995)
                     /locus_tag="IVY70_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7960000.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          381 t
ORIGIN      
        1 ttttagaaaa agcagtcgga ctttctaaaa gaagttcact attcgatcct gtcgatgatt
       61 tcttcaggaa aatcgtctat ttgcttgtca attttgctgt ctttgtattt ctcttcttgg
      121 atagtcaaca aaatctttgt aagattcccc cagtctactg ctcttggcgg atcgagccaa
      181 accactactt gagatgcact ttcttcatat agattattcc tattcgtgat tataacatcc
      241 gggaactcag gctttcctgc ctctatcact tccgtgttca gattaatgta caagcttaaa
      301 tttttcttga taaattgatt ataggcagca ccataagaaa aatcgatgat gatctttact
      361 ggttcagaaa tcagttgaat gggtatgtga ttgatcaata ataataaata actgaaaaaa
      421 agaaaaaatt tatcttgaag caactctttg cgttttgacg ttagatcatt tatatactct
      481 atcaaatagc aataaaatgc tggatagttt tctctaaaaa aagaaacatc aagtggaaat
      541 tcatcagctt tattaatagt gtaatgcaaa agtttaaaat gtattttggt taactctctt
      601 cggatcaatt ggctcgtctc ttctcgcaac agattaaact gattttcaaa actttcaatg
      661 aatcgttcat tcaaccattc gattgtttca tcttcatttt ttctcaagta tttttgatcc
      721 aatttatcat gaagataaag ccaagagagt gtcttattga taaaaataac ggatttttct
      781 cctaatactc cttttaagtg gtaaaatcct atatccgaaa aaaatagcga ctgattgcta
      841 gcaatatcta aagaatcagc ttgttcagcc aacaggttga ttgctaaaaa gtggaataat
      901 ttcttttttt cataagcaga cagaggataa tactgtttta aatcttttaa cattttttct
      961 gtttttataa ttataggtag cgtatacaat tccacaaaac ttatctctgt tgagttaaaa
     1021 agattatata aaaaaagccg aatgttttta ggattccctg tcaa
//
LOCUS       DACCXO010000252         1063 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.260, whole genome shotgun sequence.
ACCESSION   DACCXO010000252 DACCXO010000000
VERSION     DACCXO010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.260"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(38..847)
                     /locus_tag="IVY70_002926"
     CDS             complement(38..847)
                     /locus_tag="IVY70_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7960001.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT          322 a          202 c          186 g          353 t
ORIGIN      
        1 ttgtcacaac ctcttttttg tggaatctaa ttctctatta tagtttaagt agtttttcca
       61 gataaacagc taaaccgtct tcctgattgc tcaaaggagt gacatcattt gcaatgcctt
      121 tgagttggtc agtaccgtta gccatagcta ctccccaacc tgcataatcc aacaattcca
      181 aatcattatg ctcgtcacca aaggcaatga cgtctttttg atcgatagac aggtagttag
      241 caatctcttg cacgcctttt gctttttgga ttccttttga aacaatctct aagatggcag
      301 taggtccacc ccatgtccga acatcgacat aactgccaaa ttgacgtgtt aattcagcag
      361 atacagctcc tgcaaaacgt ttgtctgttc ggacaagcaa agatgtagga tttgtcgtca
      421 aatttttagg agaaagcaaa ttcttctctc ctggacgaga agaagcaaaa attttttcat
      481 caaagaaatc aaaactgtca ataaaaaaag tatcacgatt ttcagcagcg ataaaatcta
      541 aatttagctg ctttttttgc gataagattt caaaagcgat ttcacgattg atcaacgttt
      601 ctctttcacc atcccagtgc tgattaggga tgtgtactaa tgcgccgtta aaattaacca
      661 tcggtgttgt taattctaat tgttggtaaa actgatggct cattcggaat gggcgtcctg
      721 tagcaatact gacatgatga ccggcattga ttgctttttt tagagtctct tttgttttag
      781 atgtaatcat cgaatcttga ttgagggtag ttccgtccag atcgatggca attaattttt
      841 tattcaataa gggtccttcc ttcctcaact agcatttgtt ttagtctagc atattttagg
      901 taattaacaa gagcaatcag ctattcctaa caaaaactac agttatggct atataaatag
      961 ctaaacataa gctaaacacg aacgttaagt attattttcg ctttttttgt taaaaaaacg
     1021 ttgaaaagcg aatgaaacgt ctatataatt tgaaattgtt cga
//
LOCUS       DACCXO010000253         1017 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.261, whole genome shotgun sequence.
ACCESSION   DACCXO010000253 DACCXO010000000
VERSION     DACCXO010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.261"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="IVY70_002927"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="IVY70_002927"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            203..276
                     /locus_tag="IVY70_002928"
     tRNA            203..276
                     /locus_tag="IVY70_002928"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Asn,seq:gtt)
     gene            316..405
                     /locus_tag="IVY70_002929"
     tRNA            316..405
                     /locus_tag="IVY70_002929"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:350..352,aa:Ser,seq:gga)
     gene            420..491
                     /locus_tag="IVY70_002930"
     tRNA            420..491
                     /locus_tag="IVY70_002930"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:453..455,aa:Glu,seq:ttc)
     gene            507..579
                     /locus_tag="IVY70_002931"
     tRNA            507..579
                     /locus_tag="IVY70_002931"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:540..542,aa:Val,seq:tac)
     gene            589..661
                     /locus_tag="IVY70_002932"
     tRNA            589..661
                     /locus_tag="IVY70_002932"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:622..624,aa:Asp,seq:gtc)
     gene            697..772
                     /locus_tag="IVY70_002933"
     tRNA            697..772
                     /locus_tag="IVY70_002933"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:730..732,aa:Phe,seq:gaa)
     gene            774..854
                     /locus_tag="IVY70_002934"
     tRNA            774..854
                     /locus_tag="IVY70_002934"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:808..810,aa:Tyr,seq:gta)
     gene            861..934
                     /locus_tag="IVY70_002935"
     tRNA            861..934
                     /locus_tag="IVY70_002935"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:893..895,aa:Trp,seq:cca)
     gene            937..1009
                     /locus_tag="IVY70_002936"
     tRNA            937..1009
                     /locus_tag="IVY70_002936"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:970..972,aa:His,seq:gtg)
BASE COUNT          241 a          228 c          272 g          276 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgctgatgtt attccggcat agctcagttg gtagtagcgc atgactgtta
      241 atcatgatgt cgtaggttcg agtcctactg ccggagttct cattttgaga aagagtattg
      301 agcttctttc actctggaga gttgtccgag aggccgaagg agcatgattg gaaatcatgt
      361 agatggtgcc aaactgtctc aagggttcga atcccttact ctccgtagca aatttacctg
      421 gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc
      481 cgtacgggtc attttaattg aatgttggag gtttagctca gctgggagag catctgcctt
      541 acaagcagag ggtcagcggt tcgatcccgt taacctccat aatgaactgg ttccgtggtg
      601 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
      661 gtcgttcttg ttattcaatt aataatttta aaatatggcg cggtagctca gttggtagag
      721 caacggattg aagctccgtg tgtcggcagt tcgattctgt cccgcgccac catggaggag
      781 tagcgaagtg gctaaacgcg acggactgta aatccgttcc ttagggttca gtggttcgaa
      841 tccactctcc tccattttat aggggcatag tttaaaggta gaacagcggt ctccaaaacc
      901 gttagtgtgg gttcaattcc tgctgcccct gccatggcga atgtggcgaa gtggttaacg
      961 caccggattg tggctccggc attcgtgggt tcgattccca tcattcgccc tttttat
//
LOCUS       DACCXO010000254         1014 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.262, whole genome shotgun sequence.
ACCESSION   DACCXO010000254 DACCXO010000000
VERSION     DACCXO010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.262"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>1014
                     /locus_tag="IVY70_002937"
     rRNA            <1..>1014
                     /locus_tag="IVY70_002937"
                     /product="23S ribosomal RNA"
BASE COUNT          272 a          229 c          310 g          203 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgt
//
LOCUS       DACCXO010000255         1011 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.263, whole genome shotgun sequence.
ACCESSION   DACCXO010000255 DACCXO010000000
VERSION     DACCXO010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.263"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(66..1011)
                     /locus_tag="IVY70_002938"
                     /pseudo
     CDS             complement(66..1011)
                     /locus_tag="IVY70_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          266 a          233 c          188 g          324 t
ORIGIN      
        1 ccgattgccc ttgacaatga gcatcctcgg atctgatttt ctccggcaga agcacaatca
       61 ttcaattacg ctgcctctcc agaggtatca gatccgcgct cattatcaag gattcgcttc
      121 gccaatctct gttgtcgaat ttggatcggt gtttcatacg ccaacgtgcc atgtaagcat
      181 agatagttcc accaatgcac ataatcaaac aattctagac gtagctgtgc cagtgtctca
      241 aattggtatt gatgcacgaa ttctactttg acagatttat gggttgattc cgcaacggca
      301 ttatcatacg gacaaccctt cctactcaat gaacgagtaa ttccaaaacc attcagaatc
      361 tcatgaatgg tttggctatc aaattccttt ccacggtctg tatgaaaaag cttgacgtct
      421 gttaaagaat acggtatccg tccaaaggct tcttttacca atgaggcatc tttcttctca
      481 ccacaggaat aaccaataat ttctcgatta aacaaatcaa gtattaagca gatataatgc
      541 cactttttcc tcacgcgaac ataagtaaga tccgtaacga tggcttccaa tggctgttct
      601 tgtgtaaagg tccgatctaa tacattcgtt gttttcgctt cattacaagc tgttcgttgc
      661 cctttaaaat gagcgatcgt ataggtagat gtcaatccgc ggcgtttcat gattcgaccg
      721 attcggcgcc gactgagttg aagcccacgc tttgctaaac acttcttcaa ttttcgggta
      781 ccgtaagcct ttcggtttcg aataaactct tcctgaacga tctcttctaa gtcggactca
      841 ttttcgatcg gtttcgcttg ataatagtag gtctgacgag aaatatttag aattttgcac
      901 atcgctgata tggaatattt atgcttgttg gcatcaatta cttgtctttt cgtccgaata
      961 tcagcgccgc ttgctttaaa atatcattct ccatttcgag ttgcttattt t
//
LOCUS       DACCXO010000256         1006 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.264, whole genome shotgun sequence.
ACCESSION   DACCXO010000256 DACCXO010000000
VERSION     DACCXO010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.264"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..197
                     /locus_tag="IVY70_002939"
     CDS             <1..197
                     /locus_tag="IVY70_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP7960002.1"
                     /translation="NNLIEQDHRPIKRRNKFYRSLRTASSTIKGMETIRGIYKKNRRN
                     GTLFGFSVSTEIKVLMGIPA"
     gene            280..897
                     /locus_tag="IVY70_002940"
     CDS             280..897
                     /locus_tag="IVY70_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7960003.1"
                     /translation="MWIFKQEIFAKLFSLDGLGWGILFFIFLFLGSMWIWFVDKKNSY
                     EKVIVKKMNRNEFDDFSKEHRVELYDSHEINSTSPSYYWVFGCEEKPLEIKLLKMLEN
                     PPVIEDFSAKNVEKQMFEQVQIRGQNKISLEPDEYAAFFLETNSGTSMPVFKVKLKMN
                     GRLCTYDLVANMRYGSTGDRRTILKFEQTFRSFLKNRVSQIFNLD"
BASE COUNT          343 a          129 c          189 g          345 t
ORIGIN      
        1 tgaacaattt aatagaacaa gaccatcgac ctattaagcg acggaataaa ttttatcgaa
       61 gtctccggac tgcctcatcc acgattaagg ggatggagac cattcgagga atatataaaa
      121 agaaccgaag aaatggaacg ctcttcggct tttcggtgtc tactgagatc aaggtattaa
      181 tgggaatacc agcctaagat atttggagtt cagagagggc gcgtttgatt ttcaaacttc
      241 gcaacagaac cattatttta aagtgggagg aaaattgttt tgtggatatt taaacaagaa
      301 atctttgcta aattatttag tttagatgga ttaggttggg gaatcttatt ttttattttt
      361 ttatttttag gatctatgtg gatctggttt gttgataaaa aaaattctta tgagaaagta
      421 atagtgaaaa aaatgaatcg aaatgaattc gatgattttt caaaggaaca tagagttgaa
      481 ttgtacgata gtcatgaaat taacagtact tctccatctt attattgggt atttggttgc
      541 gaagaaaaac cattggagat taaattgttg aaaatgcttg aaaatcctcc agtcattgaa
      601 gatttttctg caaaaaacgt tgaaaaacag atgtttgaac aggtacaaat aagaggtcaa
      661 aacaaaattt cattagaacc agatgaatat gcagcctttt ttttagaaac aaattcaggt
      721 acttcaatgc ctgttttcaa agttaaatta aaaatgaatg gtcgtttatg cacttatgat
      781 cttgttgcaa atatgcgcta tggttctact ggtgatagac gtactatttt aaaatttgag
      841 caaaccttta gaagtttttt gaaaaataga gtatcccaaa tttttaattt ggattgaaaa
      901 aattaaaaat ttggccaaaa atactacagt tttttagagc aatcatctta tgatgattgc
      961 tctttttttg ttgtttttgt gtcattcgtt gttcaaatcc tctatt
//
LOCUS       DACCXO010000257         1003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.265, whole genome shotgun sequence.
ACCESSION   DACCXO010000257 DACCXO010000000
VERSION     DACCXO010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.265"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>1003
                     /locus_tag="IVY70_002941"
     CDS             <1..>1003
                     /locus_tag="IVY70_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7960004.1"
                     /translation="GKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLVTP
                     FCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPRCL
                     LFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHYIV
                     TDMNAPYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQYR
                     QLKSLFKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQDL
                     LTAYQEKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLENLH
                     "
BASE COUNT          300 a          209 c          191 g          303 t
ORIGIN      
        1 tggcaagaag atctctatga ttctcctgaa tcgtagcggt aataaacgga cctatcttcg
       61 gttgaagaaa cagcgatacc tctgccgagc atgtaaaaaa tattttaccg ctcggacgta
      121 tctcgttacc ccattctgct ttatttctaa gcagattcat tacaagatct tagaagaatt
      181 gacagagcgt caatctataa aagcaattgg taaacattgc gatgtttctg tgacaactgt
      241 tcagcggaca ttgtccgcct tgtcatcaga tatccgtcag aatcttgagt atttacctcg
      301 gtgtctatta ttcgatgagt ttcgttctct ttctacatgg cacggtaaat tttctttttc
      361 ttgtatggac ggtgagaccg ggaaactgtt tgagatctta cctagccgcc gtaaaaaaga
      421 tctcgttgct tttttcatgc gtttttctct caaagcgagg ttgggtgtac attacatcgt
      481 aacggatatg aacgctcctt atttctcact agttaaagag tgttttccaa atgcacaagt
      541 gatcgtcgat cggtttcaca ttgttcaaca tctgaaccgc ttcttcgatg cggttcgaaa
      601 acgtgtgatg aaaacacttg atcaaaaaga cccaatacaa gctaaacagt atcgccaatt
      661 gaaatcctta ttcaaattac ttttaaaatc agaggatcgt ttagattaca atacactaac
      721 gaaaagacgc aatttcaatt ggagactatt gactgaaaca gaagtagttg accgccttct
      781 aaagatttct cctgaactta aaacagctta tcgttactac caagacctgc ttactgcgta
      841 tcaggaaaaa gatccagata cttttttcca gctgcttcga gcaatgcctg gagaagttcc
      901 attggaactg caccatatca aaaaagcctt tttcaaatac gagaaaggga ttcggctggc
      961 attactgaag aagtattcga atgcacgact ggaaaatctc cat
//
LOCUS       DACCXO010000258          999 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.266, whole genome shotgun sequence.
ACCESSION   DACCXO010000258 DACCXO010000000
VERSION     DACCXO010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.266"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(81..635)
                     /locus_tag="IVY70_002942"
     CDS             complement(81..635)
                     /locus_tag="IVY70_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7960005.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADVAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          323 a          186 c          107 g          383 t
ORIGIN      
        1 cttccgaaaa ttcagttatc ttcattaatc tcaccttcta agttttcatt atacacaaaa
       61 aatatatcct aatatctcaa ttagcctccc aatttaaaac cacgtaacga tgcccctatt
      121 tcatcttcag ttataccaat gtaccttttt gtaattgctt gcgagctatg attaaaaata
      181 aacattaagg tagcaatgtc tctagttttc ttgtagtagt gatacccaaa agtttttctt
      241 aatgtatgtg ttcctatatc atctcttcct aatttttctg ctacatctgc atatatctga
      301 tagacacgat cccttgttat atgtcgacct tgttgccgac ttggaaataa ccaatcattt
      361 tcttctttac cttcagtata taataaaatc tcttctctca aattatctat aaaaatcggt
      421 tttcgcttac ctgttttatg ttcaacaata ataggcttac ttttacctcg tatgtctcca
      481 acttttaatc ctataatatc tgacatacgt aacccattat tgattccaaa caaaaataaa
      541 aaactatctc tttctggatt acttgttctt cttagccaaa actgaaaatc ttgtatctct
      601 tcatttgtcc ttaacggttg tacattatag ctcactattg tttcccttct taaaaatatt
      661 ctgagttcat gattatatat atattttacc ataatcatga acttatatat atactctgtt
      721 aaaagtgaag aaaatcaagg ggtttagttt atgattttat atcatttcag taactcagaa
      781 tattccgact tttttgtaca aaaaaattaa ccttacttat atgaagattc taagctctta
      841 tttaaatttt agacgggcag ttatccgtag aagttcttag aatagctaat ttacataatt
      901 aaattatatg aaatttaagt ttgaacaata tactaagaac tccccttact ataaagtgaa
      961 ataaattagc atatgaataa ccgtgctacc aaacttttc
//
LOCUS       DACCXO010000259          998 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.267, whole genome shotgun sequence.
ACCESSION   DACCXO010000259 DACCXO010000000
VERSION     DACCXO010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.267"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(218..766)
                     /gene="aac(6')"
                     /locus_tag="IVY70_002943"
     CDS             complement(218..766)
                     /gene="aac(6')"
                     /locus_tag="IVY70_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP7960006.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          291 a          209 c          147 g          351 t
ORIGIN      
        1 tattaagcca aatttatgca aaaatgtaga aagccatttt cacaaaattt ttcttactac
       61 tcgaaactaa gcgacttttt cacaacctct ggtacacggt gttccaaaag tagcttcttc
      121 ggaaataagt tggaatttca caaaaattca agaagtaatt ttcgtaaatt ccttcttatt
      181 tcttgaagct gattacttct gtcacaacct ctttttatta ttgagaattt ggtcgaggaa
      241 taatcgtttt tgccatccaa atatccggtt tgtcccagcc atttgcattt ggtaagacac
      301 ctacgatttt ataacctaat ttttcataga attcatacgg atgttcacga aggttctgga
      361 tggaagccac tttatcaaat gtatgctcat acaggtccgt ttgacttaac gttgttccat
      421 ggtctaaatc atccgtacct aaataaatcg tgattcctcc tctggaagct acttcttttt
      481 ctaagtaatt gactaatcga gtacctattt ggttctttcg tcgggagctt tctacaacta
      541 atggatgcaa ttcccaacct gtgataccgt attgagggat tgcaccaata aatcctacta
      601 actcatcttg gtctaccgct gctaccgcga ttcgttctgg attcatcatt tcttctactt
      661 cttctgccga gctgtctcca tattcttccg gccaagtcag tctcagtaaa tcagaaagct
      721 gatctttcaa tactggatta ttacggtcaa attcactgat tatcatacgt cttccccctt
      781 gttcttgctt attctaacag tccttttgaa aaaacgaaat gttctcgctc aaaaatcttt
      841 agctaccaat cagtatctct attctttcat tatttatctt acttataaaa taaaaaaatt
      901 atacagaaac actttattca aaataggaaa gatcaggaaa gccatcaatt ggaggaaaaa
      961 actctgtttg ttcatcatga tatagattat cttttgct
//
LOCUS       DACCXO010000260          991 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.268, whole genome shotgun sequence.
ACCESSION   DACCXO010000260 DACCXO010000000
VERSION     DACCXO010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.268"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(21..94)
                     /locus_tag="IVY70_002944"
     tRNA            complement(21..94)
                     /locus_tag="IVY70_002944"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="IVY70_002945"
     tRNA            complement(108..181)
                     /locus_tag="IVY70_002945"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="IVY70_002946"
     tRNA            complement(209..281)
                     /locus_tag="IVY70_002946"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="IVY70_002947"
     tRNA            complement(301..374)
                     /locus_tag="IVY70_002947"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
     gene            complement(384..457)
                     /locus_tag="IVY70_002948"
     tRNA            complement(384..457)
                     /locus_tag="IVY70_002948"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(421..423),aa:Arg,seq:acg)
     gene            complement(468..553)
                     /locus_tag="IVY70_002949"
     tRNA            complement(468..553)
                     /locus_tag="IVY70_002949"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(517..519),aa:Leu,seq:taa)
     gene            complement(570..641)
                     /locus_tag="IVY70_002950"
     tRNA            complement(570..641)
                     /locus_tag="IVY70_002950"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(607..609),aa:Gly,seq:gcc)
     gene            complement(653..725)
                     /locus_tag="IVY70_002951"
     tRNA            complement(653..725)
                     /locus_tag="IVY70_002951"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(690..692),aa:Thr,seq:tgt)
     gene            complement(743..824)
                     /locus_tag="IVY70_002952"
     tRNA            complement(743..824)
                     /locus_tag="IVY70_002952"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(788..790),aa:Leu,seq:tag)
     gene            complement(832..904)
                     /locus_tag="IVY70_002953"
     tRNA            complement(832..904)
                     /locus_tag="IVY70_002953"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(869..871),aa:Lys,seq:ttt)
     gene            complement(908..980)
                     /locus_tag="IVY70_002954"
     tRNA            complement(908..980)
                     /locus_tag="IVY70_002954"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(945..947),aa:Val,seq:tac)
BASE COUNT          260 a          283 c          235 g          213 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat taacgcgccc aagaggagtc gaacccctaa ccttttgatc
      421 cgtagtcaaa cactctatcc agttgagcta tgggcgcata tttgaaatgc cgaggaccgg
      481 aatcgaaccg gtacggtgat cactcaccgc aggattttaa gtcctgtgcg tctgccagtt
      541 ccgccacccc ggcgcgattg ctttggcaaa gcggaaaacg gggttcgaac ccgcgacccc
      601 caccttggca aggtggtgct ctaccactga gctatttccg cgtgtttaaa gatgccggct
      661 aaaggacttg aaccctcgac cctctgatta caaatcagat gctctaccaa ctgagctaag
      721 ccggcatgta aaacttaaat tatgcgggtg aagggacttg aacccccacg ccgtaaggcg
      781 ctagatccta aatctagtgc gtctgccaat tccgccacac ccgcaaaaat atgagccgta
      841 cagggctcga acctgtgacc ctctgattaa aagtcagatg ctctaccaac tgagctaacg
      901 gctcatatgg aggttaacgg gatcgaaccg ctgaccctct gcttgtaagg cagatgctct
      961 cccagctgag ctaaacctcc atggataact t
//
LOCUS       DACCXO010000261          978 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.269, whole genome shotgun sequence.
ACCESSION   DACCXO010000261 DACCXO010000000
VERSION     DACCXO010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.269"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            104..>978
                     /locus_tag="IVY70_002955"
     CDS             104..>978
                     /locus_tag="IVY70_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7960007.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNN"
BASE COUNT          344 a          169 c          181 g          284 t
ORIGIN      
        1 agcaataagt cgccgattgt aaaattaagc tagacaacta aaaaagtcat ttgtgctaca
       61 ctcaaaatag ttccgaccaa agaattataa ggagtgagta caaatgacct atacccatct
      121 tacaacggat gaacttgtaa taatagagtc ttatttcaaa atgaatcaat ctgttgctaa
      181 aactgcccat tgcttgaatc gttcaagaca aacgatccat aaagtatatc tgttcttcaa
      241 gcaaggaaaa tcagccttag aatattatca acagtataag aaaaacaaat caaactgtgg
      301 tagacgtccg cttgttttac ccgaggaaca atcagaatat attcaaagaa aggttgttca
      361 aggatggaca cccgatgtga ttgttggtcg tgcagcgttt cctattagtt gttctgctcg
      421 taccatttat cgtatgttca aaaaggggct atttgattct tctgacttac cgatgaaagg
      481 caagcgtaaa ccgaatggac atcaagaaag acgtggaaaa caaactttcc gccgctctat
      541 tcatgaacgt gaaaaggatt atagccaatt ctcaaatgag tttggtcacc ttgaaggtga
      601 cactatcgta ggtctgaaac ataaaagtgc tgtaattacc ttagttgaac gattatcaaa
      661 agttatcatc acattgaaac cgtgtggtag acaagcgatt gatattgaaa aaaaattaaa
      721 tcaatggttt gaatctgtac cgaaaaacct attcaaatcc atcacttttg attgtggaaa
      781 ggaattttca aattggaaac agatcagtaa tgtcaatgat attgccattt atttcgctga
      841 tccaggaacg ccgtctcaaa gaggcctaaa cgagaattct aacggattgt tacgtagaga
      901 tggtttattg aaatctatgg atttcaattc agtagatgaa ttttttattc aatctgtcgc
      961 atctaaacga aataatat
//
LOCUS       DACCXO010000262          954 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.270, whole genome shotgun sequence.
ACCESSION   DACCXO010000262 DACCXO010000000
VERSION     DACCXO010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.270"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            130..>954
                     /locus_tag="IVY70_002956"
     CDS             130..>954
                     /locus_tag="IVY70_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7960008.1"
                     /translation="MCRISIIVPVYNVEKYLKKCVDSILGQTFTDFELLLIDDGSTDN
                     SGSICDELAKTDNRIKVIHKENGGLSDARNIGIEVAKGDFIGFIDSDDYIDEDMYAFL
                     YNNILKYDAELSMCGIYDVHKNKEIKKLTPFSQLVTKSEAIELVLDGKLVVANAVSKL
                     YKKELFENVRYPKGKIAEDAAVILKIINQCNKIHVDTSQKYYYYHRGDSITSKKFTKK
                     DFDIIDVWNENEKWIIKNYPQMYDKVHTRVCWAYFVVLDKLVLTEEKGLYQEKKQVR"
BASE COUNT          391 a           87 c          178 g          298 t
ORIGIN      
        1 atcgcgcaaa atagctatta ttgctctgag ctttagagta gattggtata ggaaattggt
       61 tgaacaaaaa aataaaaaaa attagaatgt ttaaattagt aaacaaaaga aaaggaattg
      121 aaaactaaaa tgtgcagaat aagtataata gttccagttt ataatgttga aaaatattta
      181 aaaaagtgtg tcgattcaat tctaggccaa acatttaccg attttgaatt attattaatt
      241 gatgatggtt ctacagataa ttcaggaagt atatgcgatg aacttgcgaa aacagacaac
      301 cgaataaaag tgatacataa ggaaaatggg ggtctaagtg atgccagaaa tattggtatt
      361 gaggtagcaa agggagattt tattgggttt atagatagtg atgattacat tgatgaagat
      421 atgtatgcat ttttatataa caatatattg aaatatgatg cagagttatc aatgtgtggt
      481 atttatgacg tccataagaa taaagaaatt aaaaagttaa ctcctttttc tcaattggtt
      541 actaaatctg aagcaattga attagtatta gatggtaagc tagttgttgc taatgcagta
      601 agcaagctat ataaaaaaga attatttgag aatgtgagat atccaaaagg aaaaattgct
      661 gaagacgcag cagtaatact gaaaattatc aatcagtgta ataaaatcca tgttgatact
      721 tctcaaaaat attattatta tcatcgtgga gatagtatta ctagtaaaaa atttacaaaa
      781 aaagattttg atataattga tgtatggaat gagaatgaaa aatggattat taaaaattat
      841 cctcaaatgt acgataaagt acatactaga gtttgttggg cttactttgt tgtgttagat
      901 aaattagttt taactgaaga aaaaggactg tatcaagaaa aaaaacaagt aaga
//
LOCUS       DACCXO010000263          947 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.271, whole genome shotgun sequence.
ACCESSION   DACCXO010000263 DACCXO010000000
VERSION     DACCXO010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.271"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            2..661
                     /locus_tag="IVY70_002957"
     CDS             2..661
                     /locus_tag="IVY70_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP7960009.1"
                     /translation="MIGLMLVIFSGKQYADNILLWGGEDNIEKINNNLTILDTALENK
                     EQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKTNLENQLSAKDS
                     ELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQSTINSLNQQINQM
                     LSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTANKAQQIVNEHTI
                     Q"
BASE COUNT          422 a          113 c          138 g          274 t
ORIGIN      
        1 aattatagga ttgatgttag taattttctc cggaaagcaa tatgctgata atattttatt
       61 atggggaggg gaagataata ttgaaaagat caacaacaat ttaactatat tagacacagc
      121 tctagaaaat aaagagcaaa aaattagtat tttaaattca caattgtctt caaacaacca
      181 aactcttgaa caactaagaa ctaatattga agtttccaaa caaaaagtgg catctttaga
      241 aaacgacaag aatcaattag tatcagagaa aactaattta gaaaatcaat tgtcagcaaa
      301 agactctgaa ttgcaaggaa aacaaaatga aattaatgcg aaaatagacg aaattaacca
      361 aaaagtagct gaaataaatc gattaaatag cgattggaat aatcgtttac aaaatgaaaa
      421 gaacaaattt aatgaagaac aatctactat taatagttta aaccagcaaa ttaatcaaat
      481 gttgagtgaa aaaaataaca ttcaaaatca actgcaagct actcagcaaa agctagaaga
      541 agaaaagtca aaaaatagta gtttgaataa ttacattgaa aagcttcaaa aagctaaaaa
      601 tgatgtagag gataccgcaa ataaagcaca acaaattgta aatgaacata caatccaata
      661 gatatagttt taatttaaca aatggtatgt ttggagataa tgagatagaa tataaatgtc
      721 ataatattat acaatctaaa caccgtttcc ttttctattt ttgatgacat cagtatatag
      781 ctactcgaaa gagtagtctt tttattattc tcgcttatta ttgatatgaa aatttcgatt
      841 tgacaaaagg taccgaataa ttttgtaata tgaattatta tcaagaagta attatgatga
      901 taacatatgg aaactagtta gtaataataa caggtgatta aactttt
//
LOCUS       DACCXO010000264          943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.272, whole genome shotgun sequence.
ACCESSION   DACCXO010000264 DACCXO010000000
VERSION     DACCXO010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.272"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            2..>943
                     /locus_tag="IVY70_002958"
     rRNA            2..>943
                     /locus_tag="IVY70_002958"
                     /product="23S ribosomal RNA"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DACCXO010000265          906 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.273, whole genome shotgun sequence.
ACCESSION   DACCXO010000265 DACCXO010000000
VERSION     DACCXO010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.273"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            6..>906
                     /locus_tag="IVY70_002959"
     CDS             6..>906
                     /locus_tag="IVY70_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP7960010.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMN"
BASE COUNT          310 a          121 c          164 g          311 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaata
//
LOCUS       DACCXO010000266          890 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.274, whole genome shotgun sequence.
ACCESSION   DACCXO010000266 DACCXO010000000
VERSION     DACCXO010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.274"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..888
                     /locus_tag="IVY70_002960"
     CDS             <1..888
                     /locus_tag="IVY70_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP7960011.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DACCXO010000267          884 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.275, whole genome shotgun sequence.
ACCESSION   DACCXO010000267 DACCXO010000000
VERSION     DACCXO010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.275"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            374..571
                     /locus_tag="IVY70_002961"
     CDS             374..571
                     /locus_tag="IVY70_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP7960012.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            573..884
                     /locus_tag="IVY70_002962"
     CDS             573..884
                     /locus_tag="IVY70_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP7960013.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
BASE COUNT          337 a           97 c          159 g          291 t
ORIGIN      
        1 tggagcttcc aaaattggat caaatatcca aatttggaag ctccatgttc gtctctatct
       61 tttttgtcta taaaaaaaga gaatgcgtag ctcatcttcg aaacgttcaa agacgccgac
      121 aaactattat gaatttatgg gtgcgagtca aagagatcga tcgtaggtat gaaagactaa
      181 tgtgattaaa gtttattatg tacgaagtgc attctcaaaa aatattaaag aatatacata
      241 gtttatatac ttaaaagtga ttaaattctt attcaggaat tattttgcta cgtttcaaga
      301 aggaattgtt ttttgaaatt taatgaatgt tagaataata gtctaaaaac attaatttag
      361 gggtgattag attatgaaac atttaaaaat tttgtctatt aaagagacac aacttatcta
      421 tgggggtacc actcatagtg gaaaatatta tggaaatgga gtgtattgca ctaaaaataa
      481 atgtacggtc gattgggcca aggcaactac ttgtattgca ggaatgtcta taggtggttt
      541 tttaggtgga gcaattccag ggaagtgcta aaatgaaaaa aaatgctaag caaattgttc
      601 atgaattata taatgatata tctataagta aagatcctaa atattctgat attcttgagg
      661 ttttacaaaa ggtatattta aaattagaaa aacaaaaata tgaattagat cccagtcctt
      721 taataaatag attggtgaat tatctatatt ttactgctta tactaataaa ataagattca
      781 ctgaatatca agaggaatta ataagaaatt tgagtgaaat tggaagaact gctggaataa
      841 atggtttata tcgagcagat tatggagata aatctcaatt ttaa
//
LOCUS       DACCXO010000268          868 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.276, whole genome shotgun sequence.
ACCESSION   DACCXO010000268 DACCXO010000000
VERSION     DACCXO010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.276"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(197..595)
                     /locus_tag="IVY70_002963"
     CDS             complement(197..595)
                     /locus_tag="IVY70_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP7960014.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(592..828)
                     /locus_tag="IVY70_002964"
     CDS             complement(592..828)
                     /locus_tag="IVY70_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7960015.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
BASE COUNT          270 a          157 c          122 g          319 t
ORIGIN      
        1 aaatcagccc actagttcta aaccactatt tcctactatt cactcttttg tattcctttt
       61 aaatcaatta tttcaaaatt aatccatatt ttccatattt ttatgaaaaa actattttgt
      121 atgattaaac ataaaaaaat cagcatattc cttaatgaca tgctgatcta attgagaaat
      181 aaagttttta attcgttcat tttattgcgg tttgtcgtaa ataactggtt atcttggact
      241 ttagtgaatc gaaatccaat gaactagtgg taatcattat gataaaatca acagcttctt
      301 gacgagaaaa agcaactgga caattattca attgaagaaa caacaacatg gcaacaagag
      361 cagttcgttt attgccatta tgaaaaggat gttttttagc taaattaatt gctaaaatcg
      421 ccgctttttc atagatttca ggatagagtt cttgattgaa tactgcttgt gaaggctgtt
      481 tgacggccat atctaatgca tttgcatctt ttacaccgat catttcgcct ggcgagtgac
      541 gtaaaataac tttcgcgttg attttgataa tttctttagt cgaaagatac ctcataaatc
      601 aaccaaatct tttaacgcgt cttcatattg attcatgatc gaatcaacta atgaatcaat
      661 atcaggttct tcttgtgctt tgatcattcg tacagaacca tcagattgaa aaacataact
      721 gattgcgtca cctgtctgca cacccatgaa ttcttttact tctggtggga tagttgttac
      781 atcgctattg ccagattttc taattttata ctttttcatt tttttcatat ttcgccaccc
      841 tctataccat tctcattcta tagtaagt
//
LOCUS       DACCXO010000269          865 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.277, whole genome shotgun sequence.
ACCESSION   DACCXO010000269 DACCXO010000000
VERSION     DACCXO010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.277"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..655
                     /locus_tag="IVY70_002965"
     CDS             <1..655
                     /locus_tag="IVY70_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP7960016.1"
                     /translation="NKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSII
                     VFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTK
                     IEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKR
                     KISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            672..>865
                     /locus_tag="IVY70_002966"
     CDS             672..>865
                     /locus_tag="IVY70_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7960017.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEAR"
BASE COUNT          319 a           93 c          161 g          292 t
ORIGIN      
        1 aaataaaata ctggaatatg ttaaaaatgg agatattcga aatttagaaa acatggtttt
       61 taatctgagt aatggaataa ttccaagtgt tagtggagat acgattcggt ctgaaaaaaa
      121 ttattctatc attgtttttg aaaagttagc acaaacgtct ataacattag gaatggatat
      181 aatagaagcg tatcaaagta gagatgcttt gatacaagaa aacgaattag ctgtatcgtt
      241 acctgaggtc ttaaaagtga gggattctgg gattgtttat tatactaagg aaatcggaaa
      301 aactaagata gagcatcttt ctcccttaat ttcttctgtc gttcaattca tcggcttaaa
      361 tatctataaa agaattactg taaaggaaat agctaattat ttttctgtaa gtgaaactaa
      421 attaagagaa agctttaaga atgaaatgca tattacgatt tataattata tctctaagcg
      481 aaaaatttct acggctaaaa taatgttgaa atcaaaccat actatcagtg aagtttcttt
      541 aggtttagga ttttcagatt cttcacactt ttcgagagta ttcaaaaagt acgcaggtgt
      601 atctcctaag caatatcaat taggtttagt ggacaatttc aacaacatta gctgagaggt
      661 tttattttat tatgagacta tatcatttac gtagtttgga ggagttgagg aaattggatt
      721 ttaacaaaaa atttttttgg gaaatttctg atttgagttg tttcgtagaa tctgagcgtc
      781 taagtaatgg taataatagt gtgttaaatg aatatgacaa agtagtaatt aataaagtaa
      841 aaagtttatg tgaagaagct agaga
//
LOCUS       DACCXO010000270          828 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.278, whole genome shotgun sequence.
ACCESSION   DACCXO010000270 DACCXO010000000
VERSION     DACCXO010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.278"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..566)
                     /locus_tag="IVY70_002967"
     CDS             complement(<1..566)
                     /locus_tag="IVY70_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
                     /protein_id="HAP7960018.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQV"
     gene            complement(590..>828)
                     /locus_tag="IVY70_002968"
     CDS             complement(590..>828)
                     /locus_tag="IVY70_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7960019.1"
                     /translation="DPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNV
                     LVRWNLVAHRLGYEDIRLKEDTAYDSTVLDQKRF"
BASE COUNT          237 a          170 c          129 g          292 t
ORIGIN      
        1 acttgtaagt tacctaactt agaaatttct tctttcagag catctaactt gtcttgtagc
       61 gttttagctt gaccagttaa atcagtaatc tgttgattta agctatccac tctacctttg
      121 atttcagcca taaaagcatc aaaagtttcg ttgtactttc gaatcaactc ttctaattgc
      181 gaaacatatt catcggcttg gccttgcgaa atgtcagaca ctcctagtga gaaaaaaatg
      241 atatcttgcg ttgttaaaat ttgattgtct tttctatatt ctacgtagca gtgtttataa
      301 tatcctgctt cactcataaa tgtgccatca agagaaaacg tgacttcttc actagttaca
      361 ctagttgcaa cattatctac gtaacggtta gatggtgttg ttccttttaa agtaaatgtt
      421 ccgccactcg tatccatctg caagccattt agaaacggtt taaccgtcac cgtaatccct
      481 ttatcacctt gacgagccat aatagctttg gtgtagttta attctttgct gaaatctaaa
      541 gccaaattat ataaactgct agccatttat atacctcctt gtcttcgttt taaaaacgtt
      601 tttggtcaag cactgtgcta tcatatgctg tatcctcttt taatctaata tcttcatacc
      661 ctagacggtg tgccactaaa ttccatctaa ctaatacgtt tggcttacta gtttcaatga
      721 tgaaatggtc aatatcttca tgagtaacag cacacaaaac tagttctgta ggtgtcacat
      781 gtgtcatata ccgacttaga tttactgttt cagcaaacat ggggtcaa
//
LOCUS       DACCXO010000271          817 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.279, whole genome shotgun sequence.
ACCESSION   DACCXO010000271 DACCXO010000000
VERSION     DACCXO010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.279"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            88..>817
                     /locus_tag="IVY70_002969"
     CDS             88..>817
                     /locus_tag="IVY70_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HAP7960020.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKG
                     ITISVPPRKNMDKSEKLDHSLLGKQ"
BASE COUNT          300 a          152 c          136 g          229 t
ORIGIN      
        1 cctagaggaa aatgtctaaa catgttggta aatcaacaaa aaaacgcata tagtagtctc
       61 aaaactccca agaaatgagg actacatatg cgttcatcaa aatatactga acattataca
      121 gatactttta taacattcaa ggaaattatg agaactgttt ctaacatata ccacaattgt
      181 gtaccagata agattaaaaa ccgaagaaat actgatcaac tgaagcaacg cgatacagtg
      241 attattgctt gtgttatatg gggcataatt aatggctata ctagccaaag agccacgtat
      301 agagcggttt gttccgtctt atttcctaat ggtgactttc ctagtaggag tcgattcact
      361 cgcttaagtt caaacttagc ttacactatc aagattattc gatacttttt catcaagaag
      421 ctcaccaaag gtgaactagt cggaattata gatagttttc ctagtccatt atgtaaacca
      481 gttagaaata gacaagcaaa actattgaat caaatagcca aagttggcta taactcaaca
      541 aaaaaatctt atttttatgg tcttaaaatt catatgatcg ttactaagac tggctttccg
      601 attacttact caatcacgaa tccaggtgtc catgacgtga aagtgttaga gactttatcg
      661 gaagaagcga atcttcccaa tatcctaggg gacaaaggat atattagcca taaaatccat
      721 gaaaagcttg cccttaaggg cattactata tccgttccgc cacgtaaaaa catggataaa
      781 tcagaaaaac tagaccacag cttacttgga aaacaaa
//
LOCUS       DACCXO010000272          816 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.280, whole genome shotgun sequence.
ACCESSION   DACCXO010000272 DACCXO010000000
VERSION     DACCXO010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.280"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            183..767
                     /locus_tag="IVY70_002970"
     CDS             183..767
                     /locus_tag="IVY70_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7960021.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          259 a          121 c          167 g          269 t
ORIGIN      
        1 cattcttttt ttctaattga aagtgttcat aaaatcaccc atttctggac actttctttt
       61 tttgctttat ttttcaaaaa aacaattcat ttcatgttca taaacccata acaatatcta
      121 tgttcagtta aactatatgg ataaagttat gttacaataa aattaatgaa ggagtgattt
      181 ttttgaaaat aggatacgcc cgggtatcta ctggtttaca aaatttggat ttacaagaag
      241 acagcctaaa taaatttggt tgtgaaaaaa tttttactga tcatatgagt ggggcaaaaa
      301 gtaatcgacc aggattagaa atggccattg attttgttcg ctctggcgat acacttgtcg
      361 tttggcgctt agatcgttta ggtagaaata tggaagattt gatttcaatc gtcaatcgac
      421 tgaatgagcg tggtgtgagt tttcatagta ttcaagaaaa tattacgatg gacaaatcaa
      481 gttctacagg acagctcatg tttcatttgt ttgcggcatt tgcagaattt gagcgtaatt
      541 tgatcctgga acgatccgca gcaggacgag cagctgctcg tgcaagagga cggtttggtg
      601 gtcgtcctga aaaattgtct gaacaagatt tggaattact gaagacttta gtggatagcg
      661 gtacacctat taagactatt gcggaacggt ggaacgtctc aagaaccacc atttatcgtt
      721 atttggcaaa aatggaggag aaaaatggaa agacaaattc tcattagttt ttgtcattta
      781 atgctgaaaa tttttgccat ttatctagaa aagtga
//
LOCUS       DACCXO010000273          804 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.281, whole genome shotgun sequence.
ACCESSION   DACCXO010000273 DACCXO010000000
VERSION     DACCXO010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.281"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            175..771
                     /locus_tag="IVY70_002971"
     CDS             175..771
                     /locus_tag="IVY70_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP7960022.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          231 a          122 c          149 g          302 t
ORIGIN      
        1 aaaaaaacaa gttattaaat aagtctttag ttggatgttt ttttgagatc aatcttttat
       61 acttatgatg aacttacaaa attgtttgtt ttctgtcata tcaaaaaatg gcgcagttag
      121 ttgcttttct agtaaagtat agttaacaaa gaacctctaa ggagttgatg aataatgaat
      181 attgctgcct ctcgaaaact gataaatttc atatccatta tcggattagg cctatcaatc
      241 gcgctgacga tttattttat taatttaggt gttttcaaag atttgaatgc gttaagagga
      301 ttagtaggtg attccatcat tttaggaccg attatttttg tcttcatcca aatcctgcaa
      361 gtagttattc caatcattcc tggagggatc agtactgcag ctggtgtcct gatatttggc
      421 ccttatgctg gatttatcta taactatatt ggaatctgca ttggttcgat tatcattttt
      481 ctgctagggc gtcgttatgg aaagccattc atactttcca tgattagcga taagacatat
      541 aacaaatata ttggctggct ggataatcaa aaccgttttg agaagctgtt tgctctagct
      601 atttttcttc cagttgctcc tgacgatgcg ttatgtttga tggctggatt gacaaacatg
      661 tcagtgaaac gatatacact aatcattttg attgctaaac cactgtcgat tttcttatat
      721 agtatggcct tgatttatgg aggtcaatat ctaagtggtc ttcttggata atatttattt
      781 tgtaaaatta gtcattgatt gaca
//
LOCUS       DACCXO010000274          796 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.282, whole genome shotgun sequence.
ACCESSION   DACCXO010000274 DACCXO010000000
VERSION     DACCXO010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.282"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>796
                     /locus_tag="IVY70_002972"
     CDS             <1..>796
                     /locus_tag="IVY70_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAP7960023.1"
                     /translation="LKEKDVVSLDKQTKDEVKKNVIGNMSSKIGGVIVMGTDNILIST
                     FVNLTAVGIYSNYSLIVTSVQNLCKQITNSITASVGNFAVSNKKKDSFELFKKHFFIN
                     HSLIFFSSILLLTLMNPFISLWVGAKYMLPMSTTILIVLNYSVQVYRNTSFVFIESFG
                     LYWSQRKKPIIEAGVNLLLSLLLLIVFDMGINGVLIGTIGSSLGFVIWYEAFIIYKTV
                     FEKRFIHFFLTFWRSFIELIISAIVVFLLNEYLANGGSTFLQFITKT"
BASE COUNT          264 a           99 c          133 g          300 t
ORIGIN      
        1 ttaaaagaga aagatgtagt ttctctagat aagcaaacaa aagatgaagt aaagaaaaat
       61 gtcataggta atatgtcctc aaaaataggc ggggttattg taatggggac agataatata
      121 ttaatttcta catttgttaa tttaacagct gtaggaattt attctaacta ttcattgatt
      181 gtcacaagtg tgcaaaattt atgtaagcaa attactaatt ctatcacggc aagtgtagga
      241 aattttgctg tatcaaataa aaaaaaggat tcatttgagc tatttaaaaa acattttttt
      301 atcaaccact cactaatctt tttttcttct atattgttat tgactttaat gaatcccttc
      361 attagcttgt gggtaggagc caaatacatg ctacctatgt caacgactat tttaattgtt
      421 ttaaattata gtgtacaggt ttatagaaat acatccttcg tttttataga gtcatttggt
      481 ttatattggt ctcaacggaa aaagcctata attgaagcag gtgtaaactt gttattatct
      541 ttgcttttgt taatagtttt tgatatggga atcaatggag tacttatagg gactatcgga
      601 tcgtctctag gatttgtgat ttggtatgaa gcatttatca tatataaaac ggtatttgag
      661 aaacgtttta ttcatttctt tttaactttc tggcgaagtt ttatagaatt aataatttcg
      721 gcaatagtcg tttttctatt aaatgaatat ttagcaaatg gaggcagtac gttccttcaa
      781 tttataacta aaacct
//
LOCUS       DACCXO010000275          790 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.283, whole genome shotgun sequence.
ACCESSION   DACCXO010000275 DACCXO010000000
VERSION     DACCXO010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.283"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
BASE COUNT          272 a          122 c          116 g          280 t
ORIGIN      
        1 gtaagtattg atgatctgtc taacttaatt tgacaattaa gattatatta aatattatac
       61 agataataac ggaatattat catcgaaaca gctaacaaaa ttgtcataaa aaacgagatg
      121 gttgatatta gaacttgtgt ttttgtttag gtctagagat aaagagagac aatttccaaa
      181 tatatgtaat gaatacaaaa agacgtttgt cgcggattac cgttacaaac gtcttttatt
      241 gattttccga gatctcatca gtcgccattt ggaatggttc actccttatc tagattcgtt
      301 taatgctcgg actgcttgtt cgattgtaat accaaacgca acgttatttt cgttttgtgc
      361 atcaatcgcc ataaaacggt tagaagttgt atcaaaagct acgcggtatt gcatatttgt
      421 ttttgtaaac gtcataaaat tcatctcctt tgtaatgtaa tttagttctt gaaagtttcc
      481 tttaaacttt cgaacttata ccaagtttag catcgtacgt tttttgatgc aattaaaaag
      541 catttatcat tttccttatt tattttttct taaatggttt agctattttc ttttaatccg
      601 ataagtagcc caatcatcaa ggttacaaat actattgctt gaatataaat catatgctca
      661 cctctttata aataacttag caatataaag aaaaaaacgc acgaacaaca taggaatata
      721 aacaaacgtt tttgtcacaa cgtctaagat cgtgttaaaa aatagttatc taaaaagcag
      781 aaagatactc
//
LOCUS       DACCXO010000276          769 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.284, whole genome shotgun sequence.
ACCESSION   DACCXO010000276 DACCXO010000000
VERSION     DACCXO010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.284"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            36..266
                     /locus_tag="IVY70_002973"
     CDS             36..266
                     /locus_tag="IVY70_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7960024.1"
                     /translation="MGFRTKRSLSSKKNWVYSQTIFYGGIVLISLLSSTLYSLNIIDV
                     STSNSISIIGIIIAAIITQLFLVFGEKKRSKK"
     gene            complement(357..722)
                     /locus_tag="IVY70_002974"
     CDS             complement(357..722)
                     /locus_tag="IVY70_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7960025.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          288 a           98 c          138 g          245 t
ORIGIN      
        1 atatatggat ttttgatagg agtagaaatt caagtatagg ttttagaact aaacgaagtt
       61 tgtcttctaa aaaaaattgg gtatactcac aaactatttt ttatggagga attgttttaa
      121 ttagtttact ttcttcaaca ctttattctt tgaatattat agatgtttcc acctcaaatt
      181 caatatctat tattggaatc attattgctg caattattac acaactgttt ttagtgtttg
      241 gagagaagaa aagaagtaaa aaatagaaaa actagtgata gttttggtga tataagaaaa
      301 acatatatgt ataaaaagaa gaaagtagct tctctttgta catatatggt ttgtttttat
      361 ggatgccgag cttttttata agataaggct accgcccata acgacccaag gatttgtgga
      421 actaagtaaa tacctgcaag aagcaagatc agctgggtcg gttttaaagg atgatagaag
      481 taacgccatt cagtaaaatt ggcgcaaaat aaataggaag tgacagtaga tataatattg
      541 gcaatgaata aagatataat acgcctgttc ctttgaaaat aatatccagc aaaaagagat
      601 aaaaatatca atgtgatgaa cccgcttacg gaatgataac gaaaatcaag cagtaaggcg
      661 aaatgcacat aaggaacaaa agataaaagc aacggaaggt ttttccagca agtttttatc
      721 atctttcatc accacctaac aagtgttatt attttgttag ttttattgt
//
LOCUS       DACCXO010000277          762 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.285, whole genome shotgun sequence.
ACCESSION   DACCXO010000277 DACCXO010000000
VERSION     DACCXO010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.285"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            192..>762
                     /locus_tag="IVY70_002975"
     CDS             192..>762
                     /locus_tag="IVY70_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP7960026.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT          263 a          114 c          139 g          246 t
ORIGIN      
        1 aaggggctgt gacaagactt ttgtcacagc cccttattgt tgtactttta acctacaata
       61 ttttttgatt ccccctgagt actatttttt ataaatgcta gtaaatcaat cttctttggc
      121 ctttttttga taaaaaaaat actagatgta agataaaagt gtaaaaacga tttgttttag
      181 gaggggaaac gatgtgtacc gcaatcactt atgcaacgaa agaccattat tttggaagga
      241 attttgatta tgagatgtct tataatgaag tggtcactat tactccaaga aattatcgtt
      301 tcgattttag aaaggtaaaa aatttagata aacattatgc aatgatagga atagcagctg
      361 gggtatccaa ctatccactc tattatgagg ctacaaatga aaaagggttg agtatggcag
      421 gactgaattt tcctggcaat gctgattata aagaattaca agaaggaaaa gataacgttg
      481 caccatttga gtttattcca tggatactag ggcaatgctc aaccatagac gaagctaaag
      541 aattattggc tactatcaat ctagtcaata tcgattttag tgaaaaacta cccttgtcac
      601 ctttacactg gttattagct gataaagaaa aatcgatagt aattgaaagt atgaaagatg
      661 gacttcattt atatgataat cctgtcggtg tgttgacaaa taatccgcca tttgattatc
      721 aattatttaa tctgaacaat tatcgctcgc tatcaaatgg aa
//
LOCUS       DACCXO010000278          746 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.286, whole genome shotgun sequence.
ACCESSION   DACCXO010000278 DACCXO010000000
VERSION     DACCXO010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.286"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            206..742
                     /locus_tag="IVY70_002976"
     CDS             206..742
                     /locus_tag="IVY70_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP7960027.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT          213 a          130 c          160 g          243 t
ORIGIN      
        1 tagtttttcg tatttgcagt aaatgtgaaa atatgctata gtttctgtgg gaaaagatcc
       61 caaaaataat acagagtaga aaataaagcg ttaagtgcta agaggatggg atgttgcctc
      121 ttggacgaag gacgtaatgt tcgcggtttt attttcgcat tcgctgcatg atacgatgta
      181 gcagctctta aaggagatgc taaaaatgaa gaaacaccgg ttagacgcac gtatgattgc
      241 tattatgggc ttattgattg cattgatggt cactttgtcc cgactggtag caatcgagac
      301 cccatttatt aaaatcagtg tcacatttat tccacaagtg atcatgggca ttctttttgg
      361 tcctttttgg tcagggatag gcgcagtttt agctgattta gtaggaatgg ccctcttctc
      421 gaaatctgca ttctttattg gatttacatt gaatgcattt attgaaggag caatctacgg
      481 attctttttc tatcgaaaag agatcacttg gaaaaatgcc attctggcta cgcttagtgt
      541 cacgctgatc atcaacttat ttttgacacc attatggctt gcgttgatgt accatgtgcc
      601 attgttcagc tgggtcgttt gggcgccccg cctgttaaaa acagtaatat ggttaccgat
      661 tcaatcaatt gccatttatt acgtaggacg tagtattcct tacaaaaaaa ttttaagaag
      721 tttagcgatc cacgctaaat aaaaaa
//
LOCUS       DACCXO010000279          680 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.287, whole genome shotgun sequence.
ACCESSION   DACCXO010000279 DACCXO010000000
VERSION     DACCXO010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.287"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..85)
                     /locus_tag="IVY70_002977"
     CDS             complement(<1..85)
                     /locus_tag="IVY70_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7960028.1"
                     /translation="MPRRQRRTYSKEFKQQIVDLYLAGKPCA"
     gene            161..316
                     /locus_tag="IVY70_002978"
                     /pseudo
     CDS             161..316
                     /locus_tag="IVY70_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            335..>680
                     /locus_tag="IVY70_002979"
     CDS             335..>680
                     /locus_tag="IVY70_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7960029.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKN"
BASE COUNT          266 a           70 c          126 g          218 t
ORIGIN      
        1 ctgcgcaagg cttaccagcg agatagagat cgacgatttg ttgtttgaat tcttttgaat
       61 aggttcttcg ttgacgtctt ggcatgaaaa atcctccagt gtgtttttta ttattctaca
      121 caccttattt tttctgtcta gtctagtgta gccgattcag gataaggata agtgtattaa
      181 ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat tcagttgaga attgcaatca
      241 tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt ccagtggatg caattagtta
      301 tggagtaaga aaatagaaag gagtagttag gaaagtgata aaaaatgcta tagcatatat
      361 tacaagaaag aaaaatagaa ctttaataat ttttatcata ttaactatag ttctttcttg
      421 cttgtattct tgtttaacaa taatgaaatc aagcaatgaa atagaaaagg ctttatatga
      481 aagttctaat tcttcaatat caataacaaa aaaagatggt aaatatttta atgttaatca
      541 atttaaagat attgaaaaat taaaagaaat tgaagaaaaa ataattcaat atgatggatt
      601 agcaaaacta aaagatgcta aagtagttag cggagaacaa agaataaata gagaagattt
      661 atctgacgaa tttaagaatg
//
LOCUS       DACCXO010000280          649 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.289, whole genome shotgun sequence.
ACCESSION   DACCXO010000280 DACCXO010000000
VERSION     DACCXO010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.289"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..648
                     /locus_tag="IVY70_002980"
     CDS             <1..648
                     /locus_tag="IVY70_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7960030.1"
                     /translation="SGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
BASE COUNT          226 a          110 c          128 g          185 t
ORIGIN      
        1 agcggaattt ggaacggtat caaaaatact gctttgaatc tttggaatgg aattacaagc
       61 actattagct ctaaagtaaa cgatggaaaa aatgcaattt caagcggttg gtccaatcta
      121 acaggtattg tttccgacat attcaataat gttaaaagta caattgctaa catatgggaa
      181 ggcatcaaaa agactgttag cgctccaatt gattggatta gagacaaaat cagtggcatt
      241 tttgataatt tgaatatttc tataccacat attccgttac cggaatttat catggaaggc
      301 agctttaacc cgctaaaagg tcaaatcccc catttgcgtg taaaatggca tgctaaagga
      361 ggtatcttta ctaaaccaac tttactaggt ggaatgaacg gtgtcggtga agcaggacct
      421 gaagcagttt tacctttgaa aagatctgtt ttgcaagaaa ttggtgatcg tatcttgagt
      481 agcacatcag tttcatctag ggcacaaacg attcaacctg tgaataacta cgaattcaat
      541 ttcacaattg atggtaacgc agatgaggtt actatgaagc aaacaactca acaaatcatt
      601 gatagcatta caaaaattca aaatgataat gcttcggcat ggcgttaaa
//
LOCUS       DACCXO010000281          630 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.290, whole genome shotgun sequence.
ACCESSION   DACCXO010000281 DACCXO010000000
VERSION     DACCXO010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.290"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>630
                     /locus_tag="IVY70_002981"
     rRNA            <1..>630
                     /locus_tag="IVY70_002981"
                     /product="16S ribosomal RNA"
BASE COUNT          156 a          144 c          196 g          134 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga gatagagctt
      421 ccccttcggg ggcaaagtga caggtggtgc atggttgtcg tcagctcgtg tcgtgagatg
      481 ttgggttaag tcccgcaacg agcgcaaccc ttattgttag ttgccatcat tcagttgggc
      541 actctagcaa gactgccggt gacaaaccgg aggaaggtgg ggatgacgtc aaatcatcat
      601 gccccttatg acctgggcta cacacgtgct
//
LOCUS       DACCXO010000282          608 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.291, whole genome shotgun sequence.
ACCESSION   DACCXO010000282 DACCXO010000000
VERSION     DACCXO010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.291"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..384)
                     /locus_tag="IVY70_002982"
     CDS             complement(<1..384)
                     /locus_tag="IVY70_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7960031.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYK"
BASE COUNT          180 a          111 c           86 g          231 t
ORIGIN      
        1 tttataagcc acagaataac tcacgtgatg ctgtttagca aaattttcta ctgagatata
       61 tttttcctga aagaaagcca ataccatttt catagataaa gaatccgtta tataacgaga
      121 taacagagct ttgctagaac attttccatt aatttccaga catatatcca attcactgac
      181 ggttactcgc aaatttttct ctagatcgaa cttttgtaaa tcatgtttca gcttatcgat
      241 cgtttttttc aagagaaatg tcgatagcga caactcttct tgaacattcg atattgactc
      301 tgataaattt gagcttgctt ctaaatattt aagtaatgaa taacttctgt gatcttcttt
      361 gcttaaaaaa aactcataat tcattagtca tcagctcttt ctacgtatat tactttccat
      421 gcattcacca actagctggg gataaaactg atttcctgga tccgagtcac tgttgtcagc
      481 gattcttgtt tgtataatat acatcgtcct gtcttttttt taacattttg gagagcgttt
      541 tatttggcct ttctcgcgat caaacctgtt attagaatta tatcatttac tagtatgcat
      601 ttttatta
//
LOCUS       DACCXO010000283          607 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.292, whole genome shotgun sequence.
ACCESSION   DACCXO010000283 DACCXO010000000
VERSION     DACCXO010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.292"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
BASE COUNT          234 a           79 c           77 g          217 t
ORIGIN      
        1 attagcaata tataaaagaa ggtatcaaga gtcacagtga atttattccg aaaagtgaaa
       61 agagcaaata taaaatttaa taaaaataag aactaaatag gaaaacgtag acgtaatttt
      121 ctacgttttc cttttcatat atattaaata ttacggaaaa ttatataaac taatttagtg
      181 ttttgtttgt aattcctaat tttgctccac ttttagataa tttttaggtc tatttaactc
      241 attagacatt ctatgctcat ttttctccaa atttgtttca actctttcgt acttaatttt
      301 gcgtatttga aaattcttct tttagagtac ttttattacg atactagtat gatatatatc
      361 tacagcaaac agttaaaatc ttcctaaaat aagcgaatag cattcctaaa acctataaaa
      421 tttttagatg aagcgacaag tggacaatta ataaactact tgaacatgaa attatggatc
      481 acctttcaaa tttagaaaat ggaattctta tacttattgc gcaacattta ttgattgcaa
      541 aattatgtga caaaaagaac tagtagaaga aggaataatg gcttgataat aaagtactac
      601 ctattaa
//
LOCUS       DACCXO010000284          598 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.293, whole genome shotgun sequence.
ACCESSION   DACCXO010000284 DACCXO010000000
VERSION     DACCXO010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.293"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(53..>598)
                     /locus_tag="IVY70_002983"
     CDS             complement(53..>598)
                     /locus_tag="IVY70_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAP7960032.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSLVQFRKTTVTVV"
BASE COUNT          170 a          110 c          105 g          213 t
ORIGIN      
        1 ttttttggag tggagaaatc cactcctttt ttcatgctct aaaatgatat ttttacacaa
       61 ctgttacggt tgttttacga aattggacga gactttgcca gtttaatttc tgttttatcc
      121 gttcattatt gtagtaatag atatagctat cgatggctga ttttaattcc tcgaaacagt
      181 tataaacttt cccgtgaaaa atctcttgtt ttaataagcc aaaaaaattc tccataagtg
      241 aattatctaa gcagttccct ttccgagaca tgctttgaaa gattttgtac tccttcaatt
      301 tgtttctata agctttcatt tgatacgccc aaccttgatc tgagtgaaac gtacgacgat
      361 agggacaatc gttagtggtc tggatagctt cctccaaacc ttccattacc gctaatgcat
      421 tgggcttttc agagatacga taggataaaa tctcactatt gaacatatcc ataaaaggat
      481 ctagatacaa tttcttttga cggataatgc ctttggcatc cacttcatgg tatttaaatt
      541 cagttgtatc cgtcgtgatt ttctgatggc agatactggt gtagaaacga cgatggat
//
LOCUS       DACCXO010000285          578 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.294, whole genome shotgun sequence.
ACCESSION   DACCXO010000285 DACCXO010000000
VERSION     DACCXO010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.294"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..182)
                     /locus_tag="IVY70_002984"
     CDS             complement(<1..182)
                     /locus_tag="IVY70_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7960033.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQ"
     gene            complement(192..>578)
                     /locus_tag="IVY70_002985"
     CDS             complement(192..>578)
                     /locus_tag="IVY70_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7960034.1"
                     /translation="TIGISVTGTTIPTFADSTNEPTTVVDNNQELTPLDRNGIAIGNP
                     TNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSRNWKEKRNGYWYY
                     GKLYLQSYKSAGNNWIATYSGTLTRK"
BASE COUNT          162 a           98 c           90 g          228 t
ORIGIN      
        1 ttttggtctc ttttttttct tttttcttca gaatcttcta gattagaaat tgatttttga
       61 attttttgtg tctcctgtaa agttcgctga acttgtaaag ctgattcttc tttcggtaaa
      121 ttaattttac ttagaatatc gctatcttga taaaaatttg gtgtgttcac aaattctggc
      181 ataaaaaatc cctactttct agttaaggtt cctgaataag tcgcaatcca attgtttcca
      241 gctgatttat aactttgtaa atacaatttt ccatagtacc aataaccatt tcttttttct
      301 ttccaattcc gtgattgagg aggtctagaa ggccaaccaa ctttatcaaa aatcatcgtt
      361 cctgtcactc gtatcgtttt agaagctgct cgagcaagta atggagaaac tattaaataa
      421 ttttcagtaa cagttaagtc gttagttggg ttaccaatgg ctattccgtt cctatctaaa
      481 ggagttaatt cttggttatt atctactaca gtagttggtt catttgtact atcagcaaat
      541 gtaggaattg tagttccagt tacactaatt ccaatagt
//
LOCUS       DACCXO010000286          558 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.296, whole genome shotgun sequence.
ACCESSION   DACCXO010000286 DACCXO010000000
VERSION     DACCXO010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.296"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            74..>558
                     /locus_tag="IVY70_002986"
     CDS             74..>558
                     /locus_tag="IVY70_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7960035.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     F"
BASE COUNT          212 a           91 c           90 g          165 t
ORIGIN      
        1 tttagggtag aaaaaaaagt ccattctgta aaattaaagt taccacacag aaattttata
       61 ggaggacttt ctcatgacta aatttactat agaaattatg caaacactac tcaataaagg
      121 ggatttagat gaattatttc aggatcattt agagcgagcg attaattcac ttctacaagc
      181 agagctaact gcttttttag attacgaaaa atatgatcga aatggattca attcaggaaa
      241 ttcccgtaac gggaattatt cacgtacatt taaaacagaa tatggcgagt tgaatttaac
      301 tattcctaga gatcggaatg gcgaattttc tcaacaaaca ctaccagcat ataagcgaac
      361 caatgattca ttagaaacga ccattatcca attatttcaa aaaggcatta ctatggctga
      421 aatctccaaa ttaattgaaa aaatgtatgg tcaccactat acgccacaaa caatctccaa
      481 catgagtaaa ttggtggctg aagatgtttt agcttttaaa gaaagaacgt tagaagccaa
      541 ttattccgtt atatttat
//
LOCUS       DACCXO010000287          531 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.297, whole genome shotgun sequence.
ACCESSION   DACCXO010000287 DACCXO010000000
VERSION     DACCXO010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.297"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..291)
                     /gene="tnpB"
                     /locus_tag="IVY70_002987"
     CDS             complement(<1..291)
                     /gene="tnpB"
                     /locus_tag="IVY70_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP7960036.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWL"
     gene            complement(281..475)
                     /locus_tag="IVY70_002988"
     CDS             complement(281..475)
                     /locus_tag="IVY70_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7960037.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          155 a          112 c           92 g          172 t
ORIGIN      
        1 caaccatcga acctgttggt tcgttaaggt tcgaacttcg tctttcgtcc gaggccattt
       61 taacttgccg ttttctatcc gcttataaca gagaagaaaa ccatcaccgt cccaatagag
      121 aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa acggcatctt cgtaaagctc
      181 gagttgatac tcttgttgaa taagactggc aagaccatca attccttttc gcaaatctgt
      241 ttttccgcac acaatataaa tatgtttggt ttgaagtaaa tcataaagca ttggacaaca
      301 gttctttcaa aatagcttga agaatataat tgttaatacc gttatagatc aaaacttcag
      361 aaccattctc tcttttgatg cgcatcgcta acttggcagc cgcgggttta cctttttctg
      421 gtgcactctt ttcgtttcct aattggacag gaacaatctc atttaattta tacatagaaa
      481 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag t
//
LOCUS       DACCXO010000288          528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.298, whole genome shotgun sequence.
ACCESSION   DACCXO010000288 DACCXO010000000
VERSION     DACCXO010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.298"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
BASE COUNT          182 a           71 c           58 g          217 t
ORIGIN      
        1 acttttgttt cgtgttaagt ttctttacat tcctacgcac ctcgagtgta aaatttcttt
       61 attcctccca cgctccatgt tattttatat ttcctttttc atgttatttt tatccagaaa
      121 ccgatcaagt ttttacctat tcccaaaatc catttattcg ttattttttt cactgattaa
      181 gaataattat ttgatgctta aattagctaa aaaagcgatt tgttactgat ataatcatga
      241 tattgcagct gaattaggcg gaaagataaa atgttcgcaa aatcaaagta tcaattatct
      301 tgttttttta taaatttgta agtaaaaatc taaaaaagtt ttcttttaat tggtatatgt
      361 attaaaatca catttaatca cccataaagt atgcgctttc attttttgga tgagattaat
      421 atcaataaat aacgatgtaa aaatcattta ttatgctctt ttataaggat attaattgat
      481 ctaatttgaa aaaaaatcaa aatttaaatg ttttaatatt acaaataa
//
LOCUS       DACCXO010000289          518 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.299, whole genome shotgun sequence.
ACCESSION   DACCXO010000289 DACCXO010000000
VERSION     DACCXO010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.299"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>518
                     /gene="tnpB"
                     /locus_tag="IVY70_002989"
     CDS             <1..>518
                     /gene="tnpB"
                     /locus_tag="IVY70_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP7960038.1"
                     /translation="YISLLCKEEVRELPKSNSAVGIDLGIIDFAILSTGQKFDNNKFT
                     SKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEKVMNQRTDFLNK
                     LSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYKADWYGRKIIKV
                     DKWFPSSQICSN"
BASE COUNT          192 a           80 c           90 g          156 t
ORIGIN      
        1 tatatttctt tgttatgcaa agaagaagtt cgcgaactac ctaaatctaa ttctgctgtt
       61 ggtattgacc taggtattat agactttgca attctttcta ccggtcaaaa atttgataat
      121 aataaattta cgtccaaaat ggaaaagaaa ctcaaacgtg aacaacgtaa gttgtcaaga
      181 cgtgccttat tagctaaaca aaaaggtatc aatctgtttg aagctagaaa ttatcaaaca
      241 caaaaacgta aagtagcacg attgcatgaa aaagtaatga atcaacgtac tgattttctg
      301 aataagttga gtacagaaat tatcaaaaat cacgatatca tctgtattga agacttaaac
      361 acaaaaggca tgttgcgtaa tcataaacta gcaaaaagta tttcggatgt ttcttggtct
      421 agttttgtgt ctaaattaca atacaaggct gactggtacg gtcggaaaat catcaaagta
      481 gataagtggt ttccatctag ccaaatctgt tcgaattg
//
LOCUS       DACCXO010000290          517 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.300, whole genome shotgun sequence.
ACCESSION   DACCXO010000290 DACCXO010000000
VERSION     DACCXO010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.300"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            79..>517
                     /locus_tag="IVY70_002990"
     CDS             79..>517
                     /locus_tag="IVY70_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7960039.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMIS"
BASE COUNT          163 a           94 c          115 g          145 t
ORIGIN      
        1 aagtaaccat taatcctagg caggtggttg cttcgcggtt tttctttttc tacaataaga
       61 tcaagaggtg attacacgat ggctagaaag aaaagaaatc ctgatgccga aaagttagct
      121 gaatccattc tgaatgccta tcaacctgaa tctgtcgatg acatgcaaga tgctttgaaa
      181 gatgtgtttg ggcccctttt tgaaaaaatg cttcaaggag aattgaataa tcatttaggt
      241 tatgatgccc attctaaaga gcctaaggaa cacgataacc gtcgaaatgg ctatggaact
      301 aaaacgctta aaaccagttt tggtgaagta gctattgatg ttcctagaga ccgggaagct
      361 tcctttgaac cagagttaat tcctaagaga aaacgagatg tctccgacat cgaagggaag
      421 gttctttcca tgtatgcacg aggaatgagt caacgagata ttgccgcaac cgtcgaagct
      481 atttatggct ttgatatttc ccatgaaatg atttcag
//
LOCUS       DACCXO010000291          493 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.301, whole genome shotgun sequence.
ACCESSION   DACCXO010000291 DACCXO010000000
VERSION     DACCXO010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.301"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            42..>493
                     /locus_tag="IVY70_002991"
     CDS             42..>493
                     /locus_tag="IVY70_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP7960040.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEK"
BASE COUNT          168 a           69 c          110 g          146 t
ORIGIN      
        1 ttttttattt gtaattatta taaattgttg gggttgagta aatggtcatg aatgctattt
       61 cggcgatgaa aaaaacaaaa cagcagctac atgaagcggg gtttaagctg acaccgcaaa
      121 gagaagctac tctgcttgtg cttttggaaa atgagaaaga gcatttatct gcagaagaaa
      181 tcttcttttt agtgaaaaaa aagagtccgg aaattggact tgctactgtt tatcgaaccc
      241 ttgaaatctt gacagaattg aaaattgtcg ataaagtaag ttttaatgat ggattagccc
      301 gttatgattt gagaaaagaa ggcgccaaac atttccatca tcatttacta tgcttgaact
      361 gcggcacgat cgaggaagta gaagaggatc ttttacttga agtggagcaa gtagtagaag
      421 aacgttatca ttttttagta aaagatcatc ggctaacgtt ccatggaatc tgtcaggaat
      481 gctatgaaaa aga
//
LOCUS       DACCXO010000292          488 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.302, whole genome shotgun sequence.
ACCESSION   DACCXO010000292 DACCXO010000000
VERSION     DACCXO010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.302"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            160..>488
                     /locus_tag="IVY70_002992"
     CDS             160..>488
                     /locus_tag="IVY70_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP7960041.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNR"
BASE COUNT          162 a           73 c           86 g          167 t
ORIGIN      
        1 caaactcctc tacaatttca acttttctcc attatactcc aaaataataa aaaaaactcc
       61 taaatactcc atttttatct ttacttttat gaaagcggat gctatattgt ttttggagtt
      121 gttatccaaa aataataaaa aattggagtg tttaggataa tggataaaaa gaggttttta
      181 gatcttattt caaaaggttt gattgtttcc tgtcaagcat tacctggtga accattttac
      241 aaagaggaag aaagtttaat gccttatttt gctttggctg ctcaaagggc tggagcagtt
      301 ggtatacgtg caaattctgt tcgagatatt attgaaatta gaaagtgtgt atcattacca
      361 atgattggaa ttattaaaag agattatcca ccagaatctc cattcattac tgccactatg
      421 agagaagtag atgaattagt agaaaccggt gttgaggtaa tcgctcttga ttgcactctt
      481 agaaatcg
//
LOCUS       DACCXO010000293          486 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.303, whole genome shotgun sequence.
ACCESSION   DACCXO010000293 DACCXO010000000
VERSION     DACCXO010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.303"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
BASE COUNT          156 a           93 c           57 g          180 t
ORIGIN      
        1 gcgaaagcac aaaaaataaa aaaattatta cgttattaaa aaaacagatg aaagatcagg
       61 ttttattaga tctcattctt tcatcacttg aagggattta atgaactact ttttcctctt
      121 tgtcgttttt atatattcgt attccttcaa acagtaggat agcctctttc aatgcttgat
      181 atttgaactg tccgttactt aaaaatttag atgtgccgcc ctcttcctag aacatattct
      241 tttttacttt ttcattaatt gacagaccaa tcgaacgaaa ctgtaaattt cgataggata
      301 atgattggtc aaactcattc attttcaaga catctcgatt catattcaag ataggaatct
      361 ttttatcaac atcactttat tcgtattttc ctctaccata aaagcttcac gctccacaaa
      421 attgttttat acactactat tcagcccatt caacgaccaa tgaatttttt cggctctata
      481 atttat
//
LOCUS       DACCXO010000294          474 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.304, whole genome shotgun sequence.
ACCESSION   DACCXO010000294 DACCXO010000000
VERSION     DACCXO010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.304"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>474
                     /locus_tag="IVY70_002993"
     CDS             <1..>474
                     /locus_tag="IVY70_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7960042.1"
                     /translation="LKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYP
                     KTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRA
                     YQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKV"
BASE COUNT          195 a           45 c           85 g          149 t
ORIGIN      
        1 cattaaaaaa acattataca gagccttggg aagatgaagt ttttagagta attattgaat
       61 ataacaatgt tcctattgga tatggacaaa tatataaaat gtatgatgag ttatatactg
      121 attatcatta tccaaaaact gatgagatag tctatggtat ggatcaattt ataggagagc
      181 caaattattg gagtaaagga attggtacaa gatatattaa attgattttt gaatttttga
      241 aaaaagaaag aaatgctaat gcagttattt tagaccctca taaaaataat ccaagagcaa
      301 taagggcata ccaaaaatct ggttttagaa ttattgaaga tttgccagaa catgaattac
      361 acgagggcaa aaaagaagat tgttatttaa tggaatatag atatgatgat aatgccacaa
      421 atgttaaggc aatgaaatat ttaattgagc attactttga taatttcaaa gtag
//
LOCUS       DACCXO010000295          460 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.305, whole genome shotgun sequence.
ACCESSION   DACCXO010000295 DACCXO010000000
VERSION     DACCXO010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.305"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(20..91)
                     /locus_tag="IVY70_002994"
     tRNA            complement(20..91)
                     /locus_tag="IVY70_002994"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="IVY70_002995"
     tRNA            complement(113..200)
                     /locus_tag="IVY70_002995"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="IVY70_002996"
     tRNA            complement(225..298)
                     /locus_tag="IVY70_002996"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="IVY70_002997"
     tRNA            complement(312..382)
                     /locus_tag="IVY70_002997"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="IVY70_002998"
     tRNA            complement(384..459)
                     /locus_tag="IVY70_002998"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DACCXO010000296          441 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.306, whole genome shotgun sequence.
ACCESSION   DACCXO010000296 DACCXO010000000
VERSION     DACCXO010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.306"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
BASE COUNT          147 a           85 c           66 g          143 t
ORIGIN      
        1 atacaaaaag tattatttcg agaaagataa atattttcaa aaaccttttc ataatcctca
       61 ccctttctta cactgataaa tcatgcgtct aatattcaaa ttatactcga tatgctttag
      121 ctaatcagcc aactctgttt gtctactatc attatatcaa tagacaatat ctgctcccgt
      181 ctttatgatc gtgccaaagc aatcaaaaga aaattgcgaa tcacttagat cgaaagttta
      241 ctcaaacaaa agcattgtca gcggttgtca cggatttaac ttatgttcgg gtcgggacaa
      301 attgggctta tatttgtttg atgattgatt tattcaatcg tgaaattatt ggccattctt
      361 gcggaaataa aaaagatgcg cagcttgtaa aaagggccat tcaaagcatt ccttactcac
      421 ttcaagaaat cgaactcttc c
//
LOCUS       DACCXO010000297          436 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.307, whole genome shotgun sequence.
ACCESSION   DACCXO010000297 DACCXO010000000
VERSION     DACCXO010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.307"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
BASE COUNT          150 a           82 c           73 g          131 t
ORIGIN      
        1 tgtctgctac cccggcctaa tctggcaagg ggaggaggag atataatgaa ataccttttt
       61 gattttgttt ttgcaatcaa gccatttgca gaggtaactg cacactacat ctgcaagtgg
      121 cttgattgca aaattgccaa aaaagacagt aagcctacca gttaattcta taatatagaa
      181 acgtaaaaaa aaccccatca gcctacacgc tgatggggtt tgatgatata atgaaatacc
      241 tctttgttac tcataattat accataatta gtcacgaatg caaccaatag aaaaaactac
      301 ttagaacggt agatcatcgc taataacaat gcattgtcca ccaaatggat tttgtgaagc
      361 attaaataca ttgttattaa ttgtcgctgt ttttaactag gcctctcccc ttctattata
      421 aaagaagata tatatt
//
LOCUS       DACCXO010000298          408 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.309, whole genome shotgun sequence.
ACCESSION   DACCXO010000298 DACCXO010000000
VERSION     DACCXO010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.309"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            17..>408
                     /locus_tag="IVY70_002999"
     CDS             17..>408
                     /locus_tag="IVY70_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7960043.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTP"
BASE COUNT          150 a           73 c           68 g          117 t
ORIGIN      
        1 aaaagaggac ttccctatga atgattttac tacagaaatt gtgcaaactc tagtcactaa
       61 aggcgattta aatgaattat tccgttcgca cttagaaaaa gcgataaaca cactcctacg
      121 gactgaatta acggcttttt tagattacga aaaatatgat cgcactggtt ttaattcagg
      181 taattcgaga aacggttctt actttcgatc aatcaaaacc gaatatggtg aattaacatt
      241 ggaaatacct agagatcgta atggtgagtt taaacaacaa actttaccag cctacaaaag
      301 aacaaacgat acattggaaa ccactattat ccatttattc gaaaaaggtg ttacgatgtc
      361 tgaaattgct gatttgatcg aaaaaatgta cggtcatcac tatactcc
//
LOCUS       DACCXO010000299          408 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.310, whole genome shotgun sequence.
ACCESSION   DACCXO010000299 DACCXO010000000
VERSION     DACCXO010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.310"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..390
                     /locus_tag="IVY70_003000"
     CDS             <1..390
                     /locus_tag="IVY70_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7960044.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          158 a           73 c           64 g          113 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggag
//
LOCUS       DACCXO010000300          407 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.311, whole genome shotgun sequence.
ACCESSION   DACCXO010000300 DACCXO010000000
VERSION     DACCXO010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.311"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>407
                     /locus_tag="IVY70_003001"
     CDS             <1..>407
                     /locus_tag="IVY70_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7960045.1"
                     /translation="VSNGGSSNAGANTGSNTGNTSGSSSSSSSSSNTASGTKYTVKAG
                     DSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQQLTVSNSGSTNSTSGNTGSSTNTNNS
                     SSVNTGSSSSQGTYTVKAGESVWSVANKNGITM"
BASE COUNT          137 a           87 c           84 g           99 t
ORIGIN      
        1 tcgtatcaaa tggcggttct tctaatgctg gagcaaatac tggctcaaat actgggaata
       61 caagtggctc tagttcgtcc agctcaagtt cttctaatac agcttcagga acgaaatata
      121 cagttaaagc cggagactct gtttggagcg tctcaaataa atacggaatt acgatggctc
      181 agctaatcca atggaacaat atccaaaata actttatcta cccaggacaa caactgactg
      241 tttcaaacag tggatcaaca aacagcacgt caggtaatac aggttcgtca accaatacaa
      301 ataacagcag ttcagttaat actggcagca gttcgtctca aggcacttat accgtgaaag
      361 caggtgaatc tgtttggagc gtggcaaata aaaacggcat cacgatg
//
LOCUS       DACCXO010000301          400 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.312, whole genome shotgun sequence.
ACCESSION   DACCXO010000301 DACCXO010000000
VERSION     DACCXO010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.312"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            125..>400
                     /locus_tag="IVY70_003002"
     CDS             125..>400
                     /locus_tag="IVY70_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7960046.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
BASE COUNT          157 a           69 c           69 g          105 t
ORIGIN      
        1 ccgcactatg ccattacatt tatatctacg cgaattgtaa aattaagtta gaaaaataaa
       61 aaggcattta tggtacactc aaattgtatt tccgacgaaa gaaaacaaag gagtgtaacc
      121 ataaatgacc tacaaacatc ttaccataga cgaactgaca atgatagaat catattatct
      181 tcaacataat aagccggttg aaatcgctaa ccgaatggga cgtgctatac aaactattta
      241 taatgtagtc aataagttca agcaaggcaa gactgctctt gattattggc accagtataa
      301 agaaaataag aaaaaatgtg gtagaaaagt cattcaatta cctgctcatg aagtagatta
      361 cattaaagag aaagtcactc ttggttggac gcctgacgtc
//
LOCUS       DACCXO010000302          392 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.313, whole genome shotgun sequence.
ACCESSION   DACCXO010000302 DACCXO010000000
VERSION     DACCXO010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.313"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            115..>392
                     /locus_tag="IVY70_003003"
     CDS             115..>392
                     /locus_tag="IVY70_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP7960047.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQM"
BASE COUNT          139 a           64 c           64 g          125 t
ORIGIN      
        1 atattttgat tatcaaagta ttttctgtga acagaactat agaattccct aaaacttgtc
       61 cattatttca aaatttgtta tagtcttttt attcaaagcg taaaaggaga tcctatgtta
      121 aaagaaaaaa tgaaaactct ccccatgctg ccgggtgtct atcttatgaa agaccgagat
      181 ggtaaggtca tctatgttgg taaagcaaaa cggctcaaaa atagagtaag cagctatttt
      241 catcagaata aacagcattc aaaaaaagta ttacgtatga ttcatcatat aacagatttt
      301 gatttcgtcg tagtagacac ggaattagat gctttattgc tcgagtgtca attgatccaa
      361 cattaccgtc ctttttataa ccgacaaatg aa
//
LOCUS       DACCXO010000303          379 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.314, whole genome shotgun sequence.
ACCESSION   DACCXO010000303 DACCXO010000000
VERSION     DACCXO010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.314"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..188)
                     /locus_tag="IVY70_003004"
     CDS             complement(<1..188)
                     /locus_tag="IVY70_003004"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7960048.1"
                     /translation="MAFQVYLQRVYILSTGVIIGHYAEMGLNRIAYAGLMGDFSFAGL
                     YLATGCLYAFLKYVDLKN"
BASE COUNT          160 a           71 c           37 g          111 t
ORIGIN      
        1 caatttttta aatccacata ttttagaaaa gcatatagac agcctgtagc aagatataac
       61 cctgcaaaac taaaatctcc catcaaacct gcatatgcaa ttctatttaa acccatttct
      121 gcataatggc ctattattac accagtagat aaaatatata ctcgttgcaa atatacctga
      181 aacgccatag atactactcc tatgaaatag aaattccacc caatcatagt taagtttttg
      241 ctatacattt taaaacgttt tcctattaaa aaactacaga taaacaacga aatagtcatt
      301 aattgcttta atgcatctat taaaaaaggt aagaacatag acgtcgatat tccaaaacaa
      361 ataaataaaa ataaaataa
//
LOCUS       DACCXO010000304          372 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.denovo.315, whole genome shotgun sequence.
ACCESSION   DACCXO010000304 DACCXO010000000
VERSION     DACCXO010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.denovo.315"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            10..>372
                     /locus_tag="IVY70_003005"
     CDS             10..>372
                     /locus_tag="IVY70_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7960049.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKL"
BASE COUNT          146 a           55 c           72 g           99 t
ORIGIN      
        1 ggaacaacaa tggctaaaac aacaacagta acaacttttg acaatataag tattaaaaga
       61 atttctttta attttaagaa tgcaacaaat gcaatttcaa ctgattgtaa tggacaatta
      121 gatggcgaaa cagaaatgca gagaattgta aaaaaatgcg gttcaacaga agtaaaatcg
      181 aaatctaaac caatcaatat gacggtaaca attactgcac atgtaccgat ggaagtttat
      241 cgacgcttca atgggttgaa acaagatgaa cgtattaaat cgggaattta ctcttatggt
      301 cctgattccg taggggaaga tttctctctt gctgcagaga tcgtggatga tttcgaagaa
      361 aaaaataagc tg
//
LOCUS       DACCXO010000305        34387 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.guided.019, whole genome shotgun sequence.
ACCESSION   DACCXO010000305 DACCXO010000000
VERSION     DACCXO010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.guided.019"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            296..571
                     /locus_tag="IVY70_003006"
     CDS             296..571
                     /locus_tag="IVY70_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7960050.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            584..2662
                     /locus_tag="IVY70_003007"
     CDS             584..2662
                     /locus_tag="IVY70_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP7960051.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2822..4708
                     /locus_tag="IVY70_003008"
     CDS             2822..4708
                     /locus_tag="IVY70_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7960052.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4720..5667
                     /locus_tag="IVY70_003009"
     CDS             4720..5667
                     /locus_tag="IVY70_003009"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP7960053.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5687..6214
                     /locus_tag="IVY70_003010"
     CDS             5687..6214
                     /locus_tag="IVY70_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7960054.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6277..6930)
                     /locus_tag="IVY70_003011"
     CDS             complement(6277..6930)
                     /locus_tag="IVY70_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP7960055.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7062..7904
                     /locus_tag="IVY70_003012"
     CDS             7062..7904
                     /locus_tag="IVY70_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7960056.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8062..8958
                     /locus_tag="IVY70_003013"
     CDS             8062..8958
                     /locus_tag="IVY70_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7960057.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8961..9674
                     /locus_tag="IVY70_003014"
     CDS             8961..9674
                     /locus_tag="IVY70_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP7960058.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9694..10212
                     /gene="msrA"
                     /locus_tag="IVY70_003015"
     CDS             9694..10212
                     /gene="msrA"
                     /locus_tag="IVY70_003015"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7960059.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQDYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10209..10436
                     /locus_tag="IVY70_003016"
     CDS             10209..10436
                     /locus_tag="IVY70_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP7960060.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10588..11139
                     /gene="lepB"
                     /locus_tag="IVY70_003017"
     CDS             10588..11139
                     /gene="lepB"
                     /locus_tag="IVY70_003017"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7960061.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11222..11764)
                     /locus_tag="IVY70_003018"
     CDS             complement(11222..11764)
                     /locus_tag="IVY70_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP7960062.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(12001..12399)
                     /locus_tag="IVY70_003019"
     CDS             complement(12001..12399)
                     /locus_tag="IVY70_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7960063.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12659..13519
                     /gene="ylqF"
                     /locus_tag="IVY70_003020"
     CDS             12659..13519
                     /gene="ylqF"
                     /locus_tag="IVY70_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP7960064.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13512..14279
                     /locus_tag="IVY70_003021"
     CDS             13512..14279
                     /locus_tag="IVY70_003021"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP7960065.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14335..15192
                     /gene="dprA"
                     /locus_tag="IVY70_003022"
     CDS             14335..15192
                     /gene="dprA"
                     /locus_tag="IVY70_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP7960066.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15314..17392
                     /gene="topA"
                     /locus_tag="IVY70_003023"
     CDS             15314..17392
                     /gene="topA"
                     /locus_tag="IVY70_003023"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP7960067.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17361..18737
                     /gene="trmFO"
                     /locus_tag="IVY70_003024"
     CDS             17361..18737
                     /gene="trmFO"
                     /locus_tag="IVY70_003024"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP7960068.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18935..19840
                     /gene="xerC"
                     /locus_tag="IVY70_003025"
     CDS             18935..19840
                     /gene="xerC"
                     /locus_tag="IVY70_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP7960069.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19877..20425
                     /gene="hslV"
                     /locus_tag="IVY70_003026"
     CDS             19877..20425
                     /gene="hslV"
                     /locus_tag="IVY70_003026"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP7960070.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20439..21839
                     /gene="hslU"
                     /locus_tag="IVY70_003027"
     CDS             20439..21839
                     /gene="hslU"
                     /locus_tag="IVY70_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP7960071.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21857..22648
                     /gene="codY"
                     /locus_tag="IVY70_003028"
     CDS             21857..22648
                     /gene="codY"
                     /locus_tag="IVY70_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP7960072.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22730..23605
                     /locus_tag="IVY70_003029"
     CDS             22730..23605
                     /locus_tag="IVY70_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP7960073.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(23737..24375)
                     /gene="plsY"
                     /locus_tag="IVY70_003030"
     CDS             complement(23737..24375)
                     /gene="plsY"
                     /locus_tag="IVY70_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP7960074.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            24502..24936
                     /locus_tag="IVY70_003031"
     CDS             24502..24936
                     /locus_tag="IVY70_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP7960075.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25102..27147
                     /gene="parE"
                     /locus_tag="IVY70_003032"
     CDS             25102..27147
                     /gene="parE"
                     /locus_tag="IVY70_003032"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP7960076.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            27159..29609
                     /gene="parC"
                     /locus_tag="IVY70_003033"
     CDS             27159..29609
                     /gene="parC"
                     /locus_tag="IVY70_003033"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP7960077.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            29886..32117
                     /gene="pflB"
                     /locus_tag="IVY70_003034"
     CDS             29886..32117
                     /gene="pflB"
                     /locus_tag="IVY70_003034"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP7960078.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            32361..33122
                     /gene="pflA"
                     /locus_tag="IVY70_003035"
     CDS             32361..33122
                     /gene="pflA"
                     /locus_tag="IVY70_003035"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP7960079.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            33207..34133
                     /locus_tag="IVY70_003036"
     CDS             33207..34133
                     /locus_tag="IVY70_003036"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP7960080.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
BASE COUNT        11998 a         5938 c         7269 g         9182 t
ORIGIN      
        1 tcaattctga ttcatcagtc ccataaatta tagagccgat ttttgtcgca gccccatttt
       61 tttgttgtga taaaagaaca agaaaggcag gaatgggaaa gtaaacatcc ttttgtcaga
      121 aacttttatc tttcttaagt tttacttgat atctgatact atttggaata gaatgaggcg
      181 ttgaaaaatt tatggaaatt tctaactagc aataaacgat gaaacaatga aatatttttc
      241 atccaaaaag aactttgcta taatgatgga agtttgtgta taggagatct ggaaaatgag
      301 agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc tatggcgccc tttcgatgga
      361 tgaccgccgt caaatacttt gtaagttgcg taaaagaaat atattgatgt atcgtcagtt
      421 ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa cgtgtacagt gtgaattaga
      481 ggggaaccaa acacaggttg aagtagtcga aaccaaaatt ttaaagaaaa aagaacaatt
      541 tttaaaaatg cttacacaaa ataaaaaata aaggggatgg acgatggaat ttgctgagtt
      601 agttattgta tttgcagtaa cgatcacctt atcgaacata gctagccgca ttttgccaat
      661 gattccagct ccactgatcc aaattttcct tggagtggta ttaggtctga cagagtgggg
      721 acaatcaatc gattttgaac ctgaattgtt cttagttatg attattgcac cattgttatt
      781 tagagaagga gaaaaagcag atatttcttc gattctcaaa aatttcggga caattttgtt
      841 cttggcattt ggaggagtca ttttgaccct tgtcggggta ggtgcgacgt tatcattcct
      901 gttgccaagt gtcccacttg ctgcctgctt tgcttttgga gcagcgttag gtccgacaga
      961 tgcggtagca gtcagttctc tttctggacg agtgaatatc cctaaaaagg cgatgcatat
     1021 tttggaagga gaaggtcttt tgaatgatgc atctggtgtt actgccttcc agtttgctct
     1081 tggtgcgctg atcaccggat ctttttcagc agtaaacgcg gggatgagtc tagtagtttc
     1141 tagtattggg ggagctttga tcggcttttt gcttgtctgg ttcaaacaaa aaataattca
     1201 tttgatcgaa aaagcatcag cacaagatgt cacagggtat ctgctgatcg aacttttgct
     1261 tccttttcta gcctatgtat tggcagaggc atttgatgta tctggaatca ttgctgcagt
     1321 ggcagcaggt attctccaag cttctggttt taggaaaatc tctgtgtttg atgcagaatt
     1381 gtcgagcttg tcccacagta catggacaac tattgctttt acactgaatg cattagtatt
     1441 tatattttta ggaatcgaat tgacgcaagt gttctcgcca gtatggggag acggtttata
     1501 tccaaacggt cttttattag cgattatcgt attaatctct gtcatgcttt ttgtgatcag
     1561 attcatttct atcagtcttt tttatgtatt caaagatggc agtaaaaagt tcaaaaaaca
     1621 gttgaacgag atcttgatct taacatttgg cggtgtaaag ggaacagtca gtttggctac
     1681 gatttttatt ttgccatttt caatcaataa tatgatgttt taccagcggt ctttgctttt
     1741 gtttctgacg gcgggtgtca tcttagtgac tttggtcata gggatcattg tcttgcctat
     1801 gctgacggaa acagaggaag cgaaaagcac ggatttgaat gctttgatga ttttagaaga
     1861 ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat cgtaatacaa aagaattttt
     1921 agccactgaa gcagtgatag aaaattatca agaacggata cgtgatctgt acttggaaga
     1981 tttaaccgat gatgaaaagc aagaagtaca agaaatacaa gcactgattt tgtcgatcga
     2041 acgggatgga ttggatgaaa gttatcgttc gggcaagctt tcttctaatg gttatcgttt
     2101 ttactcccgt tttctttctc gattcgaaca ttctatcaca agccagattt tgtcttttat
     2161 cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc gttctccatc cgaaaatgtt
     2221 ttggcaaaga agatatgctg atcgtcagac atttattagt gatgaagata tccaagaaat
     2281 cagagagaca tatcaaaaaa atactcagtt gattattgaa agtttagata acttgactga
     2341 tatttatgat gaaacgatgg ttcaattttt tatccagcaa cgcaaacttg aaggaataaa
     2401 aatgaactcg gggaatttga tttcttcgtg gatgatccag caagatagtc tgtttacgaa
     2461 aaaaatgcta agaggttatt atcttgaaag aaaaatcatt gatgaatacg aagttgctga
     2521 aaaaatcacg acgttttctg cgaacagcta tcgcaggaat atcaatttgt tagaatctta
     2581 tacgatgaac aaaccatctg ataacttttc tttccgattt gcgtttcgag caaagtcaaa
     2641 aaaaacgagt ccaataaaat aaaaaggtaa aaattttcaa gttttataaa aaacaaaaca
     2701 tatcagaaca ggatcgaatc ggtcctgttc tttttttgag aacacaaaaa gaacaaatac
     2761 caaaaaaatt ttcctgaagt tcactatttt tatgatacaa tatccaagat aaggagggaa
     2821 catgttgaaa gaattaaaag taactgacct caaaaaaact tatggtgaaa aagatttgtt
     2881 tgaccagatt tcttttttgg tccacgaaaa agaccgtatc gggttgattg ggacgaacgg
     2941 gacgggtaaa acaagtctct taaatattat tgccggtatc gatagcagcg acggtgatcg
     3001 gcaaacagtg ttttatccaa ctgattaccg tattggttat ctttctcaag cagctgaatt
     3061 ttccgatgaa ttaactgttc tccaagcagt ctttcaagga aacagcccct tgatccaaac
     3121 tgttcgagca tatgaggaag cattgatcga actaggggaa aacggagaag accctgatgt
     3181 gcaaaaacgt tatgctaaag cagaagaaca aatgaataaa gaagatgctt ggactacaga
     3241 taccaacgca aagattattt tgcaaaaatt aggaatcagt gaattagata aaaaaatcag
     3301 tactttatca ggtggtcaga aaaaacgagt tagtctggcg caagtgttga ttgatgaacc
     3361 cgatttacta ttactagacg aaccaacaaa ccatttagat tatgaagcga tcgagtggtt
     3421 ggaaaactat ttgaaacaat accgtggcgc actattgatg gttacccacg atcgttattt
     3481 tctagataga gtagcaaacc gaatcttcga actttcattt ggaaaattat acgagtataa
     3541 aggaaattat gaagcttacg tcttagaaaa agcagaacgt gatcgtgtag aagtagaaca
     3601 agaagaaaag cgcaagcggc tttataaaca ggaattagct tggatgaggg ctggcgtaca
     3661 ggcacgtggc accaagcagc aagcacggat caaccgtttc gaagatctaa aggagaactt
     3721 gtaccaagta aaccaagaag atgatttaga gctgaaccta gcgacacaac gtttagggaa
     3781 aaaagtactg gaaatcaaag atggcagtta ccgtataaat gaccaaacac tgctagaaca
     3841 tttagattta ttgattcaat ctcgggaaag actaggtatt actggtaaaa atggtgcggg
     3901 aaaatcgaca ttgcttaata ttctagctgg cagaattccc ctggatagcg gtacaatgtc
     3961 tatcggtgaa acggttcatc ttgcgtatta tacacaagaa aatgaagaaa tggcaccaga
     4021 taaacggatg attgcttact tacaagaagc tgctgaagaa gcaaaaacag cagatggcag
     4081 tcaaatcgga gtagcagaat tacttgaacg atttttattt ccaagattta tgcatgggac
     4141 actgataggc aaactttcag gcggagaaaa acgccggtta tttttgttga aactgctgat
     4201 tcagcaacct aatgttttat tattggatga accgaccaat gatttggaca tagcaacatt
     4261 gacgattttg gaagattatt ttagaagttt tccaggtgct gtgatcactg tttcccatga
     4321 ccgttatttc ttggacaagg tagcagataa gttattagta ttccaaggaa acggaaaaca
     4381 agaactttat tacggaaaca tgtccagtta tttactgaag caaaaagaga cccagcagcc
     4441 tgctgaaaaa gcgaaaccaa aaatacagag caaggaacct gctgaaaaga agaaattatc
     4501 ttacatggaa caaaaagaat gggaaacgat tgaagatgag attgcagaac ttgaagaaaa
     4561 aatctcattg ctccaagaag agatgaatca tcaaggagat aatttcactc gattacaaga
     4621 attgcaaaac gacgtatctg aaacagaagc acaacttgaa gaaaaaatgg cacggtggga
     4681 atacttgagt gaatgggtag aagattagga ggaaaaagga tggaacaagc gtatcttgac
     4741 ttaggaagaa aattgttgga agaagggcat cgtaaagaag acagaacagg cacaggcaca
     4801 ttaagcttat tcggatacca aatgcggttt gatctgcaaa aagggtttcc tttgttaaca
     4861 acgaaacgag taccttttgg attgatcaaa agcgaattgc tttggttttt aaaaggagat
     4921 acgaatatcc gctatctatt acaaaataaa aaccatattt gggatgaatg ggcatttgaa
     4981 cgctacatca aaagtccaga ctataatgga ccagatatga ctgattttgg ccatagatgt
     5041 ttgacagatc ctgaattcaa tgaagaatat aaaaagcaga gcaagctttt ttgcgagaag
     5101 atcctaaccg atgattcctt tgctgaaaca tacggcgatc ttggacacat ctacggctat
     5161 caatggcgtc actgggaaac aaaagacggt ggctttatcg atcaaatcaa acaagtgatc
     5221 gaagcaatca aaaagactcc tgattctaga agattgatcg tttctgcatg gaatccggaa
     5281 gatgttccta gcatggcttt gcctccttgt catacgatgt ttcaatttta tgttcaagag
     5341 ggacggctaa gctgccaatt gtatcaacgc agtggggatg tgtttctagg cgttccgttc
     5401 aacattgcaa gttatgcgtt gctgactcac ctaattgcac atgaaacagg tttagcggtt
     5461 ggcgaatttg ttcacacatt aggagatgct catctttata tcaatcatat cgaacaaatg
     5521 aaagaacaat tgagccgaga aatacgcgca tttccgactt tagttttgaa tcaggaaaag
     5581 cagtcagtat ttgattttga gatggaagat atccaattag aaggatatga cccacatccg
     5641 gcgatcaaag ctcctatcgc tgtttagaat cagaaaggat gagaaaatgt tagctgcaat
     5701 ttgggcacag gatgaaaacg gattgattgg aaaagaagac cgtcttcctt ggcgtttgcc
     5761 aaatgatctg aaattcttca aacaaatgac agaagcaaat acactagtga tgggacgaaa
     5821 aacattcgaa gggatgggta gccgtccgtt gccgaatcgt cagacgattg ttttgacgag
     5881 agattcttcc tataaagcag aaggcgtgca tgtcatgcat agtgtggaag acgtgcttgc
     5941 tctggaaaaa gaaacagacg gtatcttttt catcgctgga gggtcagctg tttatcaaga
     6001 atttctgcca ttttgtacga tattataccg tacagtcatc catcatgtat tcgatggaga
     6061 tgcttatttt ccacctgttg actggtctga ttggtcactg atcaatacta gtcaaggaga
     6121 aatggatgaa aaaaatcgtt atccgcatca atttgaaacg tatcagcgta atgagaatcc
     6181 tgtaaaaagc gggacaggcg aattgacgat ataaaaaagc tatggataga ctagctgtta
     6241 tttagagctg tcccatccat agctttattt tttttattaa gtaaagaata aaacagagaa
     6301 gtacataaaa cctgcaccta acataacaaa caaatgccaa acaacatgca tatagcggat
     6361 agaattcaag ctataaaaga atgcgcctat cgtgtaagaa agcccgcctg ctaccatcaa
     6421 aataacacca gtcattccaa gagagtgata aagaggaaca gcagccacga cacagagcca
     6481 ccccataaaa atatagatga tcgtagatac ttttgatact ctctcccgtt tatgcaacgt
     6541 caacgcttta tatataatgc caagaatcgc aaacaaccag attaatacaa ataaagtcca
     6601 acctagccat cctcggatac tcagcaaaca aaaaggagta taacttcctg caatcaaaag
     6661 aaaaatcgaa ctatgatcga atacttggaa cacctttttt gcttttgtaa aaatcaatga
     6721 atgaaacaaa gtagacgtta aaaataaaag aatcatcatc gatccataga tagcataaga
     6781 gacaatatgg agggaggaat gcaagtgggc accttttact aaaagaacca ctagtcccgc
     6841 gatactcaat ccagtcccaa ttccatgggt gactgcatta aacacttcat ttaatatttc
     6901 atacgttcga ttaaatttcg ttttttccat gaaaacggct cctttactag gtgtaattaa
     6961 tgaaaaatca ttatttgtct gttatactaa aaatagaaat tgtcttacat gtagtttaac
     7021 atgaataagg catcttgtta aagaaagaga gagagaaaat aatgacaaat gtaaaaatcg
     7081 taacagattc ttcatgtacg atggaaaaaa atgtacgcga tgctttaggc attcgtgtca
     7141 tgccgctatc catcatgatc gacggaacag tatatcccga tgatgaccaa ctagacggcg
     7201 aaagatttat ggaaatgatg gcgaaagcaa aaaatctgcc caaaacaagc caaccaccaa
     7261 ttggagaatt catctcgatg tatgatgagc taggagaaga cggcagtgaa gtgatttcga
     7321 tccatatgac aaaaggcttg agtggaacag tagaagcagc aagacaagcc agtaatctaa
     7381 caaaaacgaa agtaacggtt atcgacagtg ataccactga ccaaggatta tcttttcagg
     7441 taattcgagc agctgaatta gcaaaagaag gtaaaacagt agagaaaatc cttgaagaag
     7501 tcaaaaaagt acaagacaat acaaaattat acatcggaat atccacacta gataacttgg
     7561 taaaaggtgg aagaatcagc cgaacaactg gtttgctgtc tagtgttttg aacatacgcg
     7621 tggtgatgaa tttcgcccat tctgaattaa tccctgtaac taaggggcga ggaaagaaaa
     7681 cgttcgataa atggtttaat aatttgaaaa atgaactgaa agaattgcca aatgtgcgac
     7741 aaatcggtat ttcacatgcg gatgcaaaag aattggcgca agaattcaaa gagggtttgc
     7801 aagaattatt cccggacatg catatccctg tactgcatac gaatccggtt atcgctacgc
     7861 atactggtaa agacgcgttt gcaatcatgt attatgtaga ttagtcgaaa agaggataag
     7921 ccctcagcct gaaaggcttg cagcttattc tctttttctt tataaaatta ggaaaataat
     7981 tgttttcttt tttcataaag actacctttt tgataaaatt tttgataaca ttagaaccaa
     8041 tcgaatacaa gaggtgaaaa aatgaaatta acgaaacgac atttgatcct cgttttgttc
     8101 ccattattaa ttgcgatcat catctatgca ggtttagctg cttctatacc gaaagctggc
     8161 ccactaatga aaccagtaac tactgtgaga gaagatccaa ataaaaaaga aaaacttcac
     8221 tacatagcta tcggagattc gctgactgaa ggagtaggag atcaaaccaa gcaaggagga
     8281 ttcgttcccc ttgtcgcaaa tgatctaaaa gaccgttata atttgaccgc ggttgaaaca
     8341 gaaaactatg gtgtaaatgg tgaacgaagt gaccagatct taaagcgagt aaaaacaaaa
     8401 gaagaaattc gaaacaatat caaaacagct gattttatca cactgactgt tggcggtaat
     8461 gatttgatga aagttatcca aaataattta ttcgggttaa cggtcaattc ctttaaaaag
     8521 ccactgaaaa actatcagga aaatgtcaca gaactattgg aagaatttcg atccctcaat
     8581 aaaaccgcac ctatttacat tttaggcatc tacaatccat tttatttgaa ctttcctgag
     8641 atcactgaca tgcagacaat tgttaacaat tggaacaaag gaacagaaac ggtgacaaaa
     8701 aaaatgccga actgctactt tatcccaatc aatgatttgc tttatcaagg attgaatcag
     8761 gaagtaggtg tcacagaaga aggaacagat caaacaacta gcacgacaga aaatctaaat
     8821 ggcatcaaaa ataatgtgct ctatgaagaa gatcatttcc atccaaataa tttaggttat
     8881 caattgatgg caaatgcagt gagagatcag ctgatcgaga cacaaaacca atggttagag
     8941 aaggaggatg atcaataaac atgaaaacaa gatctgacgt atcttcaaac aaaacaaaga
     9001 aaacaaatta ctggaaattc gccttcctta tccttcttgg ggtagtactt ggttcgaccg
     9061 tatttttagg cagccgtatt tttgctaatc gggaaccaga actcccagaa ataccagctc
     9121 ttacggaaag acaaggagat cctgtcttaa cgatcaattc aaataaagaa aaagtcaatc
     9181 aaatcatcag ctttttcctt agtgagtatc aaaaagatag tgatattgaa tataaatttt
     9241 atttggaaaa tgaagcacta ctcaatggta ctttcgaagt actaggtttt cctatcgatt
     9301 tctatttata ttttgatccg tatgtgatgg aaaacggaaa tgtccaacta aaagctagaa
     9361 gtctttctat cggaacattg agtttgccaa tcaaagatgt gatgaatatg atcaagagaa
     9421 actataaatt accagattgg atcgaagtca atacagatga tctgactatc atgcttaggt
     9481 tggatcaatt ccgaatgcaa aacgggatgt atattaaagc agataaaatc gacttagtaa
     9541 atgatgacat tcggtttagt ctatatttgc caaaagatac ctctgatgct tcaaacaact
     9601 caaataccaa caaaactagc accagcagta ctgaaagcaa gacaaaagaa aataaaaaat
     9661 catcggatga gtagtattag agggggaatc ataatggaac gagcaatatt tgcaggaggc
     9721 tgcttctggt gtatgatcca gcctttcgac acgcttcctg gtatccatac agtgatgtca
     9781 ggatatactg gagggcatgt accagatccg acttatgaac aagtaaaaag ccaaacgact
     9841 ggacatacag aagcagtgga gattatctat gatccaacat tgatttcata tgaacaatta
     9901 gtagaacttt actggcagca aactgatcca actgacgctt tcggacaatt cgaggatcgc
     9961 ggtgacaatt atcgcccggt gatcttttat ttgacagaag aacagcggat cattgctgaa
    10021 aaaagtaaga aacagttagc tgaaagtgga cgtttcgaac agccaatcgt tacaaaaatc
    10081 gaaccagccc aaccatttta tctagcagaa gaagaccatc aagactatta taaaaagcat
    10141 cctgaaaatt ttaaaagaaa ccatgcacgc cgggctgcct ttatcgaaga aaattggagg
    10201 aacgaagggt gaatcgaagc ttttttcatt atttaatgac actgcgaggc ggtggcacac
    10261 atgatgcttt atctgttttc gctacagaag ccggtaaaga catccaattt cctaagcaca
    10321 gctctagcta cgaagaaatt tctaattatc tggaactgaa tgtcgactat ttgccgtcca
    10381 tggatatctt tgacgaagca tgggaaaaat atttagaaaa taatcatgac atataagaga
    10441 tagttttgta ctttatgatc aaaaaagttt tttgtccaat agcctaattg aaagaattat
    10501 ttttatttca gctatctagt cgcatttttt tgatattttt cttataataa agaaagaatg
    10561 aaaaaaagag ggatggattg atagagattg acaaaaaaac aacgctatat taatcgcttc
    10621 tggcttgtat tcaaatactt acttgtttct gtattccttg cgttcatgtt gcggggattt
    10681 ttattcatcc cggtaccagt ggaaggcaat tcaatggaaa acgtgttgaa gcaaggtgat
    10741 atggttgtga tggaaaaatt ttcggagatc agacgatttg atattgttgt ttttcaatta
    10801 gcagatggca cgatttatat caaacgagta atcggtcttc caggagaaaa tgtcagctac
    10861 caaaatgatc aattgaagat caatgggaaa gtagttaagg aaccttatct aactaaaaac
    10921 ctgaaatcag atcatgcaaa tgcatcctat acgacggact ttacactaca agagctaacg
    10981 ggacaatcca agcttccgga agataattat tttgtacttg gagataatcg aagagtttca
    11041 aaagatagtc gttcgtttgg cacgatcaat aaaactgata ttctaggtaa agcaagattt
    11101 gtctattatc cacttgatga aataaaatgg atccagtgag aaacaataaa gcgtgcagtc
    11161 tgttgagact gcacgcttta ttgtttaatc caaaatcagt ttactacctt agtacgtaaa
    11221 atcaaacagc tcgtttaaac acttttctag cctctaaagt tttgataaat gaataaacaa
    11281 ccattatttg aatagggatt tggatcatat tcctgatgaa tctacctaga aaaattactt
    11341 tgaaatctgc accaaacaaa acagacatca gccaagaatt gataaaaagg ctgactggga
    11401 tcataatcaa taattgggca gcgatgacgg aaggcaggtt tatcttttta tgatataaaa
    11461 ataagccatt aataaatcca ccaaggaaag ccgtgataag aaaagcagct tgaaacgttc
    11521 ctgaaccaaa tatcgtgaaa tttaaagtgt tcgacaacat ttcaagcaaa ccagcccaga
    11581 tcggtccata tagataaccc ataagcgcca tacttaaaaa tccgaatgtg atttgaaaat
    11641 aagcaggacc aatcgctatc ttgtataaga tgacatcaag ggctaataaa agagcagagg
    11701 tcgacaacaa tcttgcattc atttcgggca atctaaaaac gtcttttgca atcaaagtgt
    11761 tcaaaatatt ctctcctttg tcagttaata ataaaaggat accataaaat caccaatgtg
    11821 tcatgacaca ttggtgattt acatacactg gcatctaaca attaaaaaaa gggtgagaag
    11881 actgctttta taaactgtaa aaaagagatt gtacgaataa aaaaaataga ggcggagaat
    11941 acttatcgta tcatccgcct ttgttttttt gttataaaaa acgtcagatt agttatctgc
    12001 ttacatcttc atccatacgg gttctaatgt atttccgtct agatcttgga cttctaatga
    12061 aaacatttct tcttcaggca ttcccatatc tacttcgtgg aaagtaccac cattttcttt
    12121 tgcaatttga gcaaattttt ttacttcatc cgcattttca acactaaacg aaatcaaagc
    12181 accacttgtt tttttattat cagctatttc tttatctttt ataaattttt tataaaaatc
    12241 atgagagaga agcattatcc aaaaagtgtc atcccatacc attgaaccag cttcttcagt
    12301 agaaaattct tcattttttt taaaacccag cttttcataa aaagcaacgg aacgattgac
    12361 atcagcaaca ggaaaattaa caaaaaccat actattcatt tgtttcgtct cctttctgtt
    12421 ttgttcgtct atcatctctt atttaggaaa cgctgtcaaa aatttagatt catatataga
    12481 aaccaaaata aaaatttgca tcatcaaaaa agtgaagatg tgatagaatg aggactggtt
    12541 cactttcact taactaatag atggaatttc tttataataa aaaagtgaat ttatattgaa
    12601 aaagaaaaaa acaagtaaag ccggggaata ttccggtatg aaaggaatgg agagaacaat
    12661 gaccatccaa tggtttccgg gacatatggc aaaagcccga cgagaagttt ctgaaaaaat
    12721 gaaatatgtg gacatcgtat ttgaattggt agacgcacga ttacctttat cttctcgtaa
    12781 tccaatgcta gatcagatca ttcagcaaaa acctcgcctt gttttattga ataaagcgga
    12841 tctggcagat ccacaacaga caaagctgtg gcaagaatat tttcgtaaac aaggccatct
    12901 tgcattagct atcacggcac aagaaagtaa aggaattaaa ggtctagtac ctgcggctaa
    12961 agaagcattg aaagaaaaac gggcaagaga tgctgctcgg gggatcaagc ctcgcgctat
    13021 tcgggcaatg gtcttaggta ttccaaatgt tgggaaatct acgttgatga atcgactagt
    13081 aggcaagaaa attgcacaga caggaaataa gcctggcgta acaaaaggtc agcagtggct
    13141 tcgtttaggt aatgagttgg aattgttaga cacaccaggg attttatggc ccaaatttga
    13201 agatgaagaa atcggcaaaa aactagcgct gacaggcgcg atcaaagacc agcttgtcca
    13261 tttagatgat ttagcgattt acggattaga cttttttgcc cgatattatt caggaagaat
    13321 cagcgatcgt tatggtttat caatcgagca agaagaaaaa ttggcaccgg aattactgat
    13381 ggatattaca gcaaaacgtg ggtataaaga agactatgat cgtggcagcg aattagtcgt
    13441 gcatgatatt cgtcaaggaa aacttggacg atacacactg gatcgttgcg aagaaatgga
    13501 gcaaaaaagt gatgactgaa tccatccaaa gtatcaaaga acgattgaaa agcatacaga
    13561 gaatagatga tcctacacta gaatcattaa gaaatgatcc acgcaaaggc gtgcagcaag
    13621 cactggcttc ttttgaacga cgattgaaaa aggaagcatt aattaaggaa aaatatgaag
    13681 aaatgttttg ctttgaaaag caagggtacg cacaaggtca ccaatggatt gccggcattg
    13741 atgaagtagg aagaggacca ttagctggac ctgtcgtggc agctgcagtt atcttgccga
    13801 aaaatgagat catattaggg ctgaatgact ccaaacaatt atcagagaaa aagcgagaac
    13861 ttctttatca acaagttcaa gaaaaagccc tggcaatcgg gatcgggatc gttgatcagc
    13921 agacaatcga tgatgtgaat atttatgaag caagtaaatt agcgatgatc caagcagtag
    13981 aaaaattgtc cattgtacca agctatttgt tgatcgatgc gatgaccttg gatttgccta
    14041 ttcagcagga aaaaataatc aaaggggatg cacgatctgt gtcgattgct gctgcaagta
    14101 tcattgctaa agtatatcga gaccaattga tgaaagaata tgacgtgctt tatcctggat
    14161 atggtttctc gcataatgca ggttatggaa caaaggaaca cttagaagga cttgaacgac
    14221 aaggaattac gccaatccat cgtaaaagtt ttgcacctgt aaaaaaatat ttacaataaa
    14281 aaacagccgc ttttacgtat cttattagaa agagagacga aaggcggttt tatgatgtat
    14341 caaatagaag aaaatttatt gaaacttgct tatacaaaag gaatcagtag tcaaggaaaa
    14401 tgggcacttg ctaactggat gatgcagtat ccttataaag aaatcggatt tgaagacatc
    14461 attaagatag gtaaaataac caaacaccgg gagctgtttc gcaaaagttg gcaggaaatt
    14521 cacgctaact ggggcaaaat ccaatcgaaa cagtcgttta tcacttgctt tgatcccaaa
    14581 tatccccctc agctgcttca cttgacctat ccgccaattg tactgtttta taatggtgat
    14641 ctatctttac tttcttgtaa catgttaagc gttgttggtg gaagacacac ttctgcttta
    14701 gccaaaaaaa cagtttatta tttactaaaa ccagtgatag aagctggata tgtgatcgtc
    14761 agtggctgcg cgaaagggat agatacatac ggtcaccaag cagcaatctc tcatggtgga
    14821 cggacaatcg cagtcattgg aacagggata gagcaagtct atcctgctga aaatcgtgac
    14881 ttgcaagagg tgattgcaaa tgatcatctt cttttgagtg aatatcctcc tcaatccggc
    14941 ccgaaaaagc attgttttcc aatgagaaat cggattatcg cagctttgtc taaaggaaca
    15001 tgtgtctttg aagccaagaa aaaaagcggc tctctgataa cagcacagca agcattggat
    15061 cttggaagaa cagttttttc tgttccagga aatattttaa cagaacattc taccggatgt
    15121 catcatttga tccaagacgg tgcagtttgc acggtttcgg gtgaagatat cttggcagaa
    15181 tttaaagaat aacaccatac ttttcatttg cttttcttga caaacgtttt ctgaatggat
    15241 atgattagct acgatttgta tgaatcctct tttaatagta ggaacacgac aatggaaagg
    15301 agtccattca gtaatggcgt ataaatattt agtgattgta gaatcacctg caaaagccaa
    15361 gacaattgag aaatatcttg gacgaaacta taaagtaatg gccagtgttg gccatataag
    15421 agatttaccg aaaagtaaaa tgggtatcga ttttgaaaac aattatgaac cccattatat
    15481 ttctatacgc ggaaaaggcg atgtcatcaa aagcctgaaa gccgcagcaa aaaaagctca
    15541 aaaagtttac ttggcaagtg acccggatag agaaggagaa gcgattgctt ggcatttagc
    15601 gtacctactt gggttggatc tgaaagaaaa aaatcgggtg gtcttcaatg aaatcacaaa
    15661 agacgcagtc aaagcagctt ttaaggaacc aagaacgatc gatgtagatt tagtagatgc
    15721 acagcaagct cgtcgtacct tagacagaat cgttggttat tcgatcagtc ctattctctg
    15781 gcgtaaggtc aagaaagggt taagtgcagg acgtgtccaa tctgtcgctt taaaaattat
    15841 tattgaccgt gaaaaagaga tccgagaatt tgttccagaa gaatattgga gcatcgacgg
    15901 taattttaaa aaagctcgca agaaattcaa agcaaatttc tggggaatcg acggtaagaa
    15961 aaagaaatta ccagatgcac aaagtgtaaa agaagtcact gctagaattg atggaacaga
    16021 cttcgaagtt aaaaaagtag aaaagaaaga gcgcaaaaga aaccctgcga atcctttcac
    16081 tacaagtagc ctgcaacaag aagctgcccg taagttaaac tttcgaacaa gaaaaaccat
    16141 gatggtagca cagcaattat atgaaggaat cactttgggt aaacaaggaa cagtcggctt
    16201 gattacatac atgcgtactg attctacccg gattgccgat acagcaaaag cagaggccgc
    16261 agcatttatt gaagatacgt atggaaaaga attttctaga catgatcacc gaaagatcaa
    16321 aaatgctcaa ggcgcacaag atgcccatga agctgtgcgt ccaacgagtg tgcttcgtac
    16381 accagatgaa ctcaaacaat atttagataa agatcaattg aaactttata cattgatctg
    16441 gtcacgtttt gtggctagtc agatgacacc agctatttta gatacaatga aagtgacatt
    16501 agaacaaaat ggtgtcatct tcattgcgaa cggttcaaaa atcaaattca aaggattcat
    16561 gcaagtgtat gtggaaggta gagatgacgg caaagaagag aaagaaaata ttcttccaga
    16621 acttgaagaa ggcgatgtag tccaatctgt tgatattgaa ccaaaacaac attttactca
    16681 accaccagca cgttttagtg aggctacgtt gattcgtgca ctagaagaaa acggggtagg
    16741 gcgaccttcc acttatgcac caacacttga tacgatccaa agacgctact atgtaaaatt
    16801 gacacaaaaa agatttgagc cgactgaact aggagaaatt gtcaattcat taatctgtga
    16861 attcttccct caaatcgtcg atatccattt cacagcagaa atggaaggag atttggataa
    16921 aattgaagaa ggcacggaag cgtgggtcaa agtggttgat cgtttctata aaccgtttga
    16981 aaaagaactg acgaatgcgg aagaaaaaat cgaaaaaatt caaatcaaag atgaaccagc
    17041 aggattcgat tgtgatgtct gtggacatcc gatggtgata aaattaggga aatatgggaa
    17101 gttttatgct tgcagtaatt ttcctgattg tcggaatacg aaaccaatcg taaaagaaat
    17161 cggtgtgact tgcccagtat gtcatgaagg acaagtcatt gaacgaaaat ctaagaaaaa
    17221 cagactattc tatggttgtt cccgtttccc agaatgtgaa ttcacttctt gggataaacc
    17281 agtgggcaga aattgtccaa aatgtgacgg atatctggtc gagaagaaag tcaaaggcgg
    17341 taaacaagta gtctgtgtaa atggagatta cgaagaagac atccaaaaat aatagaaaag
    17401 aggttctttc attgaaaatg gtaaatgttg ttggagcagg gcttgccgga agtgaagctg
    17461 cttggcagat cgcacaagca ggagtccctg tccgtttata tgaaatgcgt ccagtcaaat
    17521 cgactgaggc gcatcacaca agtaattttg ctgaactagt atgttccaat tcattaagag
    17581 gaaacagttt ggctaatgca gtagggttat tgaaagaaga aatgcgccga ttgaattcgg
    17641 tagtaatcac ttcggcagat gaaacagcag tgccagctgg tggtgcctta gctgttgacc
    17701 gcgattcttt ttctgaaaca attacccgaa aagtcaaaga gcatccatta gtaacagtga
    17761 taaacgaaga aattactgaa ataccggaag gaatcaccgt agtagcaaca ggacctttga
    17821 cttctcatcc attagcagaa agcatcaaag cattcaatgg ttcagatggt ttttatttct
    17881 atgatgcagc tgcaccaatc gtagacaaag ctacgatcga tatggataaa gtatatttga
    17941 agtcccgcta tgacaaagga gaagcagctt acttgaactg tccgatgaca gaagaggaat
    18001 tcaaagcttt ccatgaggca ctaatcaatg ccgaagtggc agaacttaaa tcttttgaga
    18061 aagaaaaata ttttgaagga tgtatgccaa ttgaagtaat ggcacagcga ggattcaaaa
    18121 cgatgctttt cggacctatg aaaccagttg gattggaaga tcctaaaact ggcaaacgtc
    18181 cttatgctgt tatccagctg cgtcaagaca atgctgcagc ttctttatac aacatcgtgg
    18241 gctttcagac tcacttgaaa tggggcgagc aaaaacgtgt tttccggatg attcctgggt
    18301 tagaaaatgc tgaattcgtt cgctatggtg tgatgcaccg aaacagcttt atgaattcgc
    18361 cagaattgct gaaaccgact taccaatcaa cgaaacgcga agatctgttc tttgcaggtc
    18421 agatgactgg tgtagaaggc tatgttgaaa gtgcagcgag cggactactt gctggaatca
    18481 acgccgcacg cttagcaaaa gaaatggaac cagtcgagtt tccacaagaa acagctattg
    18541 gaagtatggc ttactacatt actcatgcgg aaggaaaaca tttccaaccg atgaatgcca
    18601 actatggtct gtttcctgaa ctgccagagc gcattcgaga caaaaaagca agatatgaag
    18661 caattgcaca acgagcttta aaagctaatg aggcaataaa aacagaatta gaaaaaactg
    18721 caatcccaaa tgagtaagga aactccatga agagggtgag acggatagtc tcgctctttt
    18781 tcttgcgttt ttcttaagaa tttctcaaac aaaatgtgag gaattgcgcc aattgtgaac
    18841 aaacttagaa atattggtta aattctgaca atttatttgt ctttttataa acgctgtgct
    18901 aaattattct ctgggacaaa aggaaggtga gaaaatgaca gaagccacgt gggaacaaaa
    18961 atttttgcgt tatttgattg tcgaaagagg atattcagaa aaaacgaaag aagcatatga
    19021 agaagacatt ctccatttca aacatttttt ggaggaatct ggagacgctg atctgctgaa
    19081 ggcggaatat tttacggttc gtgtctattt gagtgcatta gctgatcatc gtcctagcta
    19141 tagcagaaat tctatcagtc gcaagatagc cagcctacgt tcgttttatc agtatcttct
    19201 caaagaagaa gtgataaaag aaaatccatt ttcttacgtt catctcaaaa agaaaaattt
    19261 gagactgcca cgtttttttt atgaaaatga aatgcaagca ctttttgaca gcgtagcagg
    19321 agatagtcct ttggatttga ggaaccgcgc tttactggaa gtcctttacg ggtcaggcat
    19381 ccgtttaagt gagtgtagca gtttgtcggt atcagacatt gattttgatt cagaggtcat
    19441 gttgatccat ggtaaaggga acaaagagag atatgctcct ctaggttctt ttgctcaaga
    19501 tgctctgcaa gaatatttca gggaaggccg ccaggtattg atgacaaaat atcacgaaga
    19561 gcatgatatc gtttttgtga atcatcatgg aaaaccaatc acgcctactg gaattgaata
    19621 tgtcttgaat caaatgatca aaaaaagcag tttggatagt gatatccatc ctcacatgct
    19681 gcgccataca tttgctactc atttattaaa taatggggca gatttgcgga cagtccaaga
    19741 attgctagga catgccaatt tatcgactac ccagatctat gcccatgtaa caaaagaaag
    19801 tttacaaaaa aactatcgtt ctttccatcc aagagcgtaa aaataaggaa aagaacaaat
    19861 ttaggaggaa acaaacatgg tcgaatcacc atttcattca accactatct gtgccgtcga
    19921 aaaagacggg aaatttgcta tggctggaga tggccaagta acaatgggtg aatctgttgt
    19981 catgaaaggc actgcaaaga aagtacgacg tatctacaat ggagaagttg ttgtaggttt
    20041 cgccggaagt gttgcagatg cgtttacttt agaagaaaaa tttgaaggca aactaaacga
    20101 atataacggg aatctacaac gcgcagctgt cgaattagct caagaatggc ggacacagca
    20161 atctatgcaa aaacttgaag cgatgctgat cgtaatgaat gataaagaaa tgcttcttgt
    20221 ttcaggtaca ggagaagtta tcacaccaga tgatggtatc ttagctatcg gatcaggagg
    20281 gaattttgcg ttggctgcag cccgagcaat gaaaaactat gctcaaaacg acatgagtgc
    20341 gaaagaaatt gcagaaaatg cgttaaatat tgccgcagat atctgtatct ttactaatca
    20401 taatattatc gtagaagaac tgtaagaggt gagtagatat gtatgaatta aaacaaacac
    20461 caagacaaat cgtaagtgaa ttagatgaat atatcgtagg acagcacgca gcaaaaaaat
    20521 ctgttgccgt tgctttgcgt aaccgctatc gccgtttgca gttagatgaa aaaatgcaac
    20581 aagatattac accaaaaaat atgctgatga tcggacctac aggggtcggt aaaacagaaa
    20641 tcgcacgacg tttggcaaaa atcgtcaatg caccatttgt caaagtcgaa gcaacaaaat
    20701 ttactgaagt cggttatgtc ggaagagatg tagaatcaat ggttcgggat ttagtagaaa
    20761 atgcgatcca aatcgtagaa aaagagcagt acagccgtgt atacacaagt gctaaaaaaa
    20821 atgcagaaaa acgtttggta aaaatcttgg tacctggtat caaaaaagaa cagaaacaat
    20881 ccactaatca atttgaacaa atgatgaatc tttttaatgc tagtcagcaa aataccgctg
    20941 aaccgcaaga agaagtgaca gaagatattc gaatcaatcg ccgtacagtc ttggaacaac
    21001 tacaaaaggg tttactggat agtcgtgaag taaccattga agttgaagac caaaagaaac
    21061 ctgcaccaat gatgaacaat ggattggagc aaatggggat cgacctaaat gaaactctag
    21121 gtgctttgac accgaagaaa aaagtggaac ggacggtaac agtcaaagaa gctttagagt
    21181 tattgatcaa ggaagaatca gcaaaattag tcaaggatgc tgatatccat agtgaggcaa
    21241 tcaaattagc ggaagaaagt ggaattattt ttatcgatga aatcgataag atcacttcta
    21301 aatcacagca atctggagaa gtatccagag aaggcgtcca aagagacata ttgccaattg
    21361 tcgaaggttc acaagtaaac acaaaatacg ggatcatcca aacagatcac gtcctattca
    21421 ttgcttcagg agctttccat ttgtctaaac caagtgatct aattccagaa cttcaaggac
    21481 gcttcccgat acgggtagaa ttagatgatt tgacagctga agattttgtg aagattctga
    21541 ccgaaccaaa taatgcttta gtaaaacaat acgtcgcgtt gatcggtaca gaaaatgtgt
    21601 cagttacgtt tacaaaagaa gcaattgaac ggatcgctca aatcgctttc gatgtcaacc
    21661 gtgacacaga taatatcggc gcacgcagac tgcataccat tttggaaaaa ctactagaag
    21721 atctattatt tgaagcacca gatatgcaaa tgggagaaat aacgatcaca gaagcatatg
    21781 tggatgacaa acttggctca attgcggcaa atgaagattt aagtcgctat attttataaa
    21841 aaatcggagg acgacaatga cagacttgct aacgaaaacc cgcaagataa acacactttt
    21901 gcagcaaaag aatacttttg acatgcaagc agatttgcct tacgataaaa tggctgtcac
    21961 actaggtggt attcttgaca gcaacaccta tatcatagga agtgacggag tattactagg
    22021 ttataatgaa cgtcacgatg tgaacaatga acgaatcaaa acaatgtttg ttgagaaaca
    22081 atttcctgaa tcttatactg atacagtggc acagttgatg aaaactgaag ctaatatccc
    22141 aattactagt gatattactg catttccggt agagtcaaga gagaaatatc catttggtct
    22201 gacgactgtc gttccgatct ttggagctgg cgaacgtttg ggtacgatta tcttatctcg
    22261 tatggaacaa tcgtttgcag atgacgatct agtcttagca gaatatggag ctacggttgt
    22321 cggtatgcag atcctttatc agaagtctcg aagcatcgaa gcagatgtac ggagtgcaac
    22381 ggctgtccaa atggcaatca acacattatc ttatagcgaa ttgaaggctg tacaagctat
    22441 ctttgaagca ttggatggag atgaaggtcg tctgactgct tctaatattg ccgatagtat
    22501 cggcatcact cgttctgtga tcgtcaatgc tttacgaaaa ttggaatcag ctggaatcat
    22561 tgaatctcgt tcgttaggca tgaagggaac ttatttaaaa gtattgaatt cccgttttaa
    22621 agacgagtta gcaaaacaca gttattagaa gtaaggggac tggggcagat agaaatgttt
    22681 gttccagcct tcccatttta aaaagtaagt cgaagttgga ggaatggcaa tgactgttat
    22741 tattcaaaat gaccagctga tcgccgaaat cgctgaacac ggtgcggaat tgatcagtct
    22801 gaaatcaaaa gaacatggtt tagagtatat ctggcaaggt gatccagcgt attggggcag
    22861 acatgcaccg gttttattcc cttttgttgg acgtttaaaa gatgatcagt atgtttatca
    22921 agggaaaaca tatcaaatgg ggcaacacgg atttgctcgg gatatggatt ttgaagtcat
    22981 tgaacatgga caagaaatgg ctagtttcat cttaaggagt acgccggaga cattagaaaa
    23041 gtatccattt gattttgaat tagtgatcag ctatgaatta ggaggagacg gcatcacggt
    23101 tcattatcaa gtggcaaata ccggtgaaaa agaaatgttc ttttctatcg gtggacatcc
    23161 tgctttcaat gtacctcttg aagaaggatt gtcatttgaa gatttctatc tggcattttc
    23221 tccattgaaa tcaagattac gcctaccatt gaaaggccca ttcatcgata tggatcaaaa
    23281 gacgttaggt cagacaaata cgaatctggc tttgaatcgt gaactttttg agcaagatgc
    23341 cttggttttt gaaacaaggg gtctaaatgc gtacgccatt cgaagtgaaa aatcttccca
    23401 tagcgtaact ttaagctata aagatatgcc ttatgtaggg atctggtctc cttatccaaa
    23461 agaagcgcct ttcgtttgta tcgaaccgtg gtgcggaatc gctgacacgg tgaacagtag
    23521 tggaaacttg atggaaaaag tagggatcaa tcaactaggt gcccatgaaa tcttcaagac
    23581 aaaatattcc atcacagtga agtaagaggt tgtgaccgag aagttagcag ggaacaccgt
    23641 ttaccaaggt gtgtaaaaaa gctatcctgt aagtaataag ggagtgtgac atcgatttgt
    23701 cacactcccg ttttatctct ttggaatgaa aaagtatcag gattttttct tgctgctatt
    23761 caaaccaaac gggattcgac tttcagtatg gtttctgata cgcttgatat tgtctttatg
    23821 ccgataaaag ataaaggaag aaactaaaaa ggcaatgatc gtcagtaacc aattgggctt
    23881 agccaaaatc acaggagcta cataaggaag gacgatcgta gataaagtga tcaatactgc
    23941 actgatcata ctgctcaaac tcaccatact tgtacaaaat aaactaacga taaagatgat
    24001 acaagaataa acaaaaaata caggattaaa tcctaaaagc attcctgcac ttgtcgcaac
    24061 agcttttcct cctttgaacc cagcaaaaat cggaaatgta tggccaagaa tggcgcatac
    24121 cccaaaccaa agagggttaa tcccagaaag gtggaagatc atcggcaaac aagtagccaa
    24181 tgtacctttt aaaatatcca taagcaggac aacaatcccg gctgttttgc ctaatacacg
    24241 aaatgtattg gtcgttccag tattaccgct cccgtgttgt cggatatctt ttttgaaaaa
    24301 caatttgcca atccagacac cggaaggaat agaacctaaa agatatgcta aaataagtaa
    24361 taagatcaat ttcatttttt accctctttc aagttgaaaa ctatccttta ttttagcatg
    24421 aataggtaac aggaacaatt gaaaaaatga aagatacaag tataatagat ggaataaaaa
    24481 gaaaagcaag gggtggcaga aatgtctgta aaaaatccaa cacaagaaac gattttcaac
    24541 tatttggaag aagcaaaaaa tattgccgtc gtaggcttga gcaataaacc agaacgaaca
    24601 agttacatgg ttgcacaggt attgcaagac aatggatata aaatcatacc agtaaatcca
    24661 ttattggctg gtgaagaaat tctaggcgag aaagtctatg cgaatttgac agaaatcgat
    24721 gatccaatcg atatcgtaga tgtatttaga cggagtgaat ttttacctga aatagcaggt
    24781 gattttttga aaactgatgc taaagtgttc tgggcacaat tagggattga aaacaaagaa
    24841 gcagctgaga tgctaagaaa agcaggacgt acagatatcg taatggatcg ttgtattaag
    24901 attgaacttg gtaagttgaa agaaaaccaa aaataaaaat gaccacaaag aagagatgat
    24961 cgatagaggg gaatgtcgaa aaagcggtta ggagataatt aaaattgatt tctcgtctac
    25021 cctctttttt ttcacgacct tttctagtat gataatcgtg gtgcggtttt gccgcaaagt
    25081 attattgaag gagcgtttgc cttggcaaaa aaagtaaaca acgaatacaa tgactcgtcc
    25141 atccaagtat tggaaggatt agaggcagtt agaaaaagac cagggatgta cattggatcc
    25201 actgacagcc gaggcttgca tcatttagtc tatgaaatcg tcgataatgc agttgacgaa
    25261 gcattatcag gttatggcag tgagattgat gtgaccatcc atgaagacaa tagtataact
    25321 gttgcagata gcggacgtgg gatgcctgta ggaatgcatg cttccggaat accaacagtt
    25381 gaagtgattt ttactgtgct gcatgctggc ggaaagtttg gtcaaggagg gtacaaaact
    25441 tctggtggac tgcacggtgt aggagccagt gtggtaaatg ccttatcgaa atggctaatt
    25501 gtaacgatcg ttagagatgg cgtagagtat cagcagaaat ttaaaaacgg cggtaaacct
    25561 gacggtactt tgaaaaagat aggaaaaaca aaaaaagcaa acgggacgac tgtccatttt
    25621 ttaccagatg atacaatatt cagtacaacg aaattttcat atgaaatttt agccgaacgg
    25681 ttaagagaat ctgcctttct tctaaaaggc gttaaaatct ctctttctga tcttcgtgga
    25741 gaagaaccag tcaaggaaat ttttcattac gaagaaggaa tcaaagaatt tgttgattat
    25801 ctgaatgaag aaaaagatac attgacacca gttgtttatt tttcaggaga aaaagaaggt
    25861 atcgaagtag aggtcgctta tcaatacaat gacggttatt cagaaaatgt tttatctttt
    25921 gtcaataacg ttcggacaaa agatggggga acccatgaag ctggtatgaa agcggcgatg
    25981 acgaaatctt ataatgaata tgcgagaaaa gttggtttgc tgaaagaacg ggacaagaat
    26041 ctggaaggta gtgatttcag agaagggtta gctgctgtac tttctatccg tgtacctgaa
    26101 aatcttctgc aatttgaagg gcaaacaaaa gaaaaacttg gtacgccagt tgctcgtacc
    26161 gtagtagata acgtcatcag tgaacaaatg ggcttttatt tgcaggaaaa tagcgaaatg
    26221 agccagatgt tggtccgtaa agcaatcaaa gccagagaag cgagagaagc agcacgtaaa
    26281 gccagagaag aaagccgaaa tgggaagaag cgtaaaaaag gcgaatctct tctttcagga
    26341 aaattgacac ccgcacaatc ccgaaaccct aagaaaaatg aattgtattt agtcgaaggg
    26401 gattctgctg gcggatctgc taaacaagga agagacagaa agttccaagc aattctacct
    26461 ttacgaggaa aagtcatcaa taccgaaaaa gctaaaatgc aagatatctt gaaaaatgaa
    26521 gaaatcaata cgatgatcta tacgattggt gcaggagtcg gtcctgagtt ttctatcgaa
    26581 gactgtaatt acgacaaagt aatcatcatg accgatgcgg atacagacgg cgcccatatt
    26641 caagtattac tgcttacttt cttttatcgt tatatgaaac cgctgattga agctgggaaa
    26701 gtatatatcg cgttaccgcc tttatataaa gtatccaaag gacaaggaaa gaagcaagtc
    26761 attgaatatg cgtggacaga tgacgaattg gcagcaatga ttaaaaaagt cggcaaagga
    26821 tatatgctgc agcgatataa aggacttgga gagatgaatg cggaacaatt gtgggaaaca
    26881 acgatggatc cgacttcccg tacgttgatc cgcgtccgta tcgatgatgc tgcgcaagct
    26941 gaacgtcgag taaccacact gatgggtgac aaagtagaac ctcgaagaaa atggatcgaa
    27001 aatcacgtgc aatttacgct cgaagaagat ggcagtatct tagataaaaa agaggatact
    27061 gaaatctctc cttcagtttc taatgattta ttagatgaag aacgagcaga taaaaatgaa
    27121 aacaatcagt tatttgaagt agaataggag gtcacctgat ggaaaatcga caagaagtac
    27181 aagaattaac tttagaagaa gtgatgggtg accgcttcgg acgttattcc aaatatatta
    27241 tccaagaacg tgcactgcct gatatccgtg atggattgaa gccagtgcaa cgccggatat
    27301 tatttgccat gaacaaagat ggcaatacgt atgacaaagg attccgtaaa tcagcaaaat
    27361 ctgtcggaaa tatcatgggg aattatcacc ctcatggtga ccgcagtatt tatgaagcga
    27421 tggtccgcat gagccaagac tggaagttgc gggaagtact gatcgaaatg cacgggaata
    27481 acgggagtat ggacggtgat ccacctgctg cgatgcgtta tactgaagcc agattatcca
    27541 agttaagtgg agagatgcta gcagatatcg agaaagaaac agtcgatctc gtgtggaatt
    27601 ttgatgatac agaaaaagaa ccaacagtcc ttccagctcg atacccaaac cttctcgtca
    27661 atggatcgac aggtatatct gctggatatg caacagaaat cccgactcat aacttagctg
    27721 aagtgatcga tggcacaatc tatatgatcg atcatccaca agcgagcctt gaaaaattga
    27781 tggaatatat tcctggacct gactttccaa caggagggat cttgcaagga aaagatgaaa
    27841 tcaaaaaagc ttatgaaaca ggacgaggaa aagttattct tcgttcgaaa acaaagatag
    27901 aggcaatgaa gggtaataaa cagcaaatcg tgatcacaga aatcccatat gaagtaaata
    27961 aagcgacctt ggtgaaaaaa atggatgaga tccgtttgaa taagaaaatc gatggaatag
    28021 ctgaagtacg agatgaaagt gatagaacag gtctgcaaat tgtcgtcgaa ttgaaaaaag
    28081 acgccaatgc tcaaggaatt ttgaattatt tgttcaaaaa cactgagcta cagatcaatt
    28141 acaatttcaa tatggtcgct atcgaccata tgactccgca tcaagttgga ctgaaagaca
    28201 ttttaagaag ttatatcgag catcgaaagc aagtcataac aaaacgtagt caattcgatt
    28261 tagccaaagc tcaaaaaaga cagcatatcg tagaaggatt gatgaaagct ttatcgattc
    28321 tggatgaagt aatcgcaacg attcgtgaaa gtaaagacaa aaaagacgcc aaaaagaatt
    28381 tagtcgacgt ctttcaattt acagaagaac aagcagaagc aattgtcaca ttgcaattat
    28441 accgtttgac gaacacggat atcacagagc ttcaaaagga atccgaaagc ttgattgcgc
    28501 aaatcacaga gttgaataaa atcctttcaa atgacaaaga gcttttctct gttatgaaaa
    28561 aagaattgcg agaagtgaaa aaaaattatt cttccgctcg tctaacgaca atcgaagacg
    28621 aaatcgaaga aatcaaaatt gatacgcaag tcttagtggc acaagaagac gtaattgtca
    28681 gtgtgacgcg agaaggctat gtgaagcgaa caagccttcg ttcttatagt gcttccaaac
    28741 cagaagaaat cgggatgcga gaaggcgact acttgctata ctcaggtgaa ttgagtacat
    28801 tggaccatgt tcttctgatc acaaataaag caaatgtgat ctaccgccct gtccatgaac
    28861 tacccgattt gaaatggaaa gatgctggtg agcacatttc tcaaacaatc tccaatctgt
    28921 ctgtagacga atcgatttta gctgttttcc catatcaaaa aattcaagca gagaaaacat
    28981 ttgtattcat cagcaagaat ggattgatca agcaaacacg aatgacagag ttcgaaccat
    29041 ggcgcaccta taagagccgt ccgctcagct gtatgaagct aaaggcggaa gaagatgaac
    29101 tgattgctgt gtatctgaaa gaagatcagc caggcttaga tgttttctta gtaacgcatc
    29161 aaggaatggg tttgcgctat cctttaacag atgttcccgt tgttggagca aaggcagctg
    29221 gagtgaagtc tatgaatttg aaagatggag ataggattat caacgggctg cttgtgttag
    29281 cagaaggtga tacgccgatc gtaatcgtta ctcagcgagg agctgttaaa cgcatgcttg
    29341 ctcaagaaat cagccaaact tcacgtgcga agcggggagt gacggtcatg cgtgagctga
    29401 aaaaaaatcc gcaccgtatt atctatatgt cggaaggaag atcaaaagaa ataaccttga
    29461 tgaatcaaaa agggcagcag ttaacgattg acccaactga tttccctata ggtgatcgaa
    29521 catcgaatgg atcatttgcg atggatgaga aaaaaggtgg agaagtcatc gaagttatcg
    29581 atgctcctga gattttcatc gagtcgtaac tgttgtagta ttttaatgaa gagacgccat
    29641 ctgtattaat acaggtggcg tctctttttc attcattttt aataaatcct tatacgtttg
    29701 caaaaaaaga tgaaagtgtt cacaatgctt aaatgttggt atgtcaacgt tgatttaaat
    29761 atatgtaaat aaaacataaa aattgttaaa taaaaaacaa gaaaaccctt gcaaagatag
    29821 ggtgctgtgt tatactaatc acgtaaaaac tgttacataa aacagaaatt taggaggtcc
    29881 atacaatgga gcaatggaat ggatttaaag gtaccaaatg gaaagaaggc gtagacacta
    29941 gagactttat tcaaaataac tacactgaat atcgcggtga tgacagcttt ttggaaggcc
    30001 caacagaagc aacagaaaaa ttatgggatg aattgcaaaa gttaaatgat atccaattcg
    30061 agcgtaacgg tgtctatgat atggacacag aaatcgtatc aactattact tcacacgatg
    30121 caggctattt ggataaagat ttggaaaaaa tcgtcggtct acagacagac aaacctttga
    30181 aacaagcatt catgccgttc ggcggaatca atatggctaa caatgcgcta aaaagcaatg
    30241 gctatacacc aaacgatgaa ttgacacata ttttcactga ctggcgcaaa acacataatc
    30301 aaggtgtatt tgacgcttat actgctgaaa tgcgtgctgc tcgtaaaaac aaaatcatta
    30361 cagggcttcc agacgcttac ggtcgcggac gtatcatcgg tgactatcgt cgtgtggcat
    30421 tgtatggaat cgactatttg atggaacaaa agaaaaaaga tcatgatatg actggttatc
    30481 atacaatgag tgaagaagtg attcgtttac gtgaagaaat cactgaacaa tatcgtgcat
    30541 tagcgcaaat gaaagaaatg gctgcgaaat atggctatga tatttctaaa ccagctgcaa
    30601 atgcaaaaga agctgtccaa tggctatact tcggatattt agcagcaatc aaatcacaaa
    30661 atggtgctgc gatgtcaatc ggacgtatct cagcattcct tgatatctat atccaacgtg
    30721 atttggataa tggagtaatc acagaaaaag aagcacaaga attgatcgac cacttagtaa
    30781 tgaaattacg tatggtcaaa ttcgcgcgta cacctgaata taaccaatta ttctcaggat
    30841 tccctatctg ggctacttta tccattgcag gtatgggatt agatggtcgt tcattagtta
    30901 caaagaatga tttccgtctg cttcatacat taacaaatat gggaccttca ccagaaccaa
    30961 acttgactgt tttgtattct gaacaccttc cagaaggatt cagaacatat gctagcagaa
    31021 tcgctatcga aagttcttcg attcaattcg aaaatgataa tcagcttcgt gaacaatggg
    31081 gttctgatga ttgtgcgatt gcttgctgtg tatcaggaac agttgtcgga aaagacatgc
    31141 aattctttgg cgctcgtgct aacttggcta aaacactatt atatgccatc aatggcggag
    31201 tcgatgaagt gactggcgcc caagttggac cagaatatcg tccaatcaca tcagaaatac
    31261 tagactttga agaagtgaaa cataacttca tgaatatgat ggattggtta gcagaactat
    31321 atgtcaatac gttgaacatc attcattata tgcatgataa atattcttat gaagcagctc
    31381 agctggcttt gatggattct gacttgcaac gcacatttgc tacaggtatt gctgggatct
    31441 ctcatgcggc agacagcttg tcagcgatca aatatgcaaa agtaaaagca atccgtaatg
    31501 aaaaaggaat agcagttgat tttgaagtgg atggcgactt ccctaaatac ggaaatgatg
    31561 atgaccgtgc agatgaatta gtttcatgga tcttggaata cttcatggga caaatcaaac
    31621 gtcataaaac ttaccgcaac tcaaaaccaa caacttcatt attgacgatt acttcaaatg
    31681 ttgtttacgg taaaaataca ggaaatactc ctgatggccg tgaagctgga aaaccacttg
    31741 cgccaggagc aaatccaagt tacaatgcgg aacaaagtgg gttattagct agcctgaact
    31801 caacagctaa aatgaattac ggctgtgcat tggatggaat ttcaaatacc caaacaatca
    31861 atccttcagg tcttggaaaa gaacaagata caagaatcga aaacttgcgt aatgtcttag
    31921 atggatactt ctcaaaagga gcttatcatt tgaatgtcaa tgtattcagt aatgatttat
    31981 tgcgtgatgc aatggaaaat ccaatgaaat atccgaactt gacgatccgg gtatctggtt
    32041 atgcagttaa attccgtgac ttgacaagag aacaacaatt agatgtcttg atgagaactt
    32101 cacacgacag aatgtaaaaa gttcgtgaaa aaagtttttc gacttagcgt cgaaaggacg
    32161 ttgaatagcc atcgcatcaa aatcgaataa tcagtatgct tatttgaaag aaacgattta
    32221 caggggcagg acttgtatgt tccgtccctg taaattttac acataacgaa gcggcaattg
    32281 cgaaaaattt tgttcagaaa gcttcgcttc gacagctaac ttgtacttaa aatgaaatca
    32341 aagaaaagat gtgaacttaa atggaagaaa aaacaatcgg ctatgttcat tctatcgaaa
    32401 ctttcggttc agtagacgga cctggactgc gctttgtcgt gtttatgcaa ggatgccgca
    32461 tgcgttgcca attttgtcac aatccagata cttggaatat tggcggaggg aaagaataca
    32521 cagcagatga acttttagat aaagcagaac gcttcagacc ttactgggga gataaaggtg
    32581 gtattactgt tagtggtgga gaacctctat tgcagatcga ttttttgatc gaactattta
    32641 aaaaagcaaa agagcggaag atgcatacta cattggatac atgcggaaaa ccttttacgt
    32701 atgaagaacc tttcttttca cgttttcaag aattgatgaa gtatacagat ttactgttat
    32761 ttgacatcaa acacatcgat aatgaagagc ataaaaaact aacgcattgg gacaatgaaa
    32821 atattttaga aatggctcaa tatttgtcaa aaatcaacaa accggtttgg attcgccatg
    32881 tactagttcc tgagcgaagt gattatgatg aatatttgat ccgactagac aactttattc
    32941 aaaccttgtc aaatgtagac agagtagaga ttcttcctta tcatacgatg ggaaaataca
    33001 agtgggaaac acttggtttg aaatatccat tagaagggat cgaaccacct acaaaagaac
    33061 gtgtcgaaaa tgctaaacga ttgcttcata cagaagagta tacaaaatat ttaacgcgat
    33121 aggtcgcatc ggaaagaaga taagtattgc ttctttccgt ttttttgcta ttattaaata
    33181 ggagaaatca caaaggagcg aatgacatgt caaaaatttt agtttttggt caccaaaatc
    33241 cagatacaga tgcaatcggg gcagccattg cgttttcaca cttgcaaaat gaattaggta
    33301 aagaaacaga agcagttgct ctaggtacac ctaatgaaga aacacaatac gcattagacc
    33361 actttcagct atctgcacca cgagtagtag agtcagctaa agcagaaaat gcagatgtca
    33421 tgcttgtgga tcataatgaa ttccaacaaa gtatttcaga tatcagcgaa gtaaatattt
    33481 tagctgtagt agaccaccat cgtattgcaa actttgaaac agctaatcca ttgtactatc
    33541 gtgcagaacc agttggatgt acaagtacga ttattttaaa actatataaa gaaaaccaag
    33601 taacaattcc taaagaaatc gcaggaatca tgctctcagc tatcatttca gatacattgt
    33661 tgttcaaatc accaacatgt acgcctgaag atgaagcagc tgcaaaagaa cttgcagcaa
    33721 tcgctgaagt cgatctaaac acttatggac ttgaaatgtt gaaagcagga acaaatctaa
    33781 gtgataaatc agctgcggtt cttcttgatt tggatgcaaa aagtttccca atgggcagtt
    33841 ccaacgttcg tatcgctcaa gtcaatacag tggaccttaa tgaagtgatg gatcgacaag
    33901 cagagctaga agcagcaatg gaagatgaaa tgaaagcaaa tggctacgat ctgttcgtat
    33961 tagttgtcac aaacatctta gacagtgatt cagaattact tgttgctggt aaaggaaaag
    34021 aaaaagtaga agaagctttc caaacaactt tgaccaacaa ccgtgctttg cttaaaggtg
    34081 tcgtttcacg taaaaaacaa gtcgtacctc aattgacggc tgtatttagc taatcaaatg
    34141 gtagtatgaa gcattcaaaa tgaatggata agcgattgat ataaaaagaa acatagaaga
    34201 tactaaaggc cgaagaaatg gaggagaata acagtcaatt atgactcttg ttcttcctcc
    34261 tttttacgta aaagcctttt aagtaaaata aaacggatag aaaagagaac atatatgaac
    34321 tattattcaa aaaataagga aaacttttat caaaaactga ccgatgatcc gcttttttct
    34381 tcattga
//
LOCUS       DACCXO010000306         9921 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.guided.099, whole genome shotgun sequence.
ACCESSION   DACCXO010000306 DACCXO010000000
VERSION     DACCXO010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.guided.099"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            371..973
                     /locus_tag="IVY70_003037"
     CDS             371..973
                     /locus_tag="IVY70_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7960081.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            989..1666
                     /locus_tag="IVY70_003038"
                     /pseudo
     CDS             989..1666
                     /locus_tag="IVY70_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2178..2474
                     /locus_tag="IVY70_003039"
     CDS             2178..2474
                     /locus_tag="IVY70_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HAP7960082.1"
                     /translation="MSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTNSASGVLRAN
                     EIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLKEKVVR"
     gene            2475..2891
                     /locus_tag="IVY70_003040"
     CDS             2475..2891
                     /locus_tag="IVY70_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP7960083.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            2911..4455
                     /locus_tag="IVY70_003041"
     CDS             2911..4455
                     /locus_tag="IVY70_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7960084.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            4598..4822
                     /locus_tag="IVY70_003042"
     CDS             4598..4822
                     /locus_tag="IVY70_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7960085.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            4837..5706
                     /locus_tag="IVY70_003043"
     CDS             4837..5706
                     /locus_tag="IVY70_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP7960086.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            5687..6421
                     /locus_tag="IVY70_003044"
     CDS             5687..6421
                     /locus_tag="IVY70_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7960087.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGVADEMRRPMMLIVSAKKKM"
     gene            6454..7362
                     /locus_tag="IVY70_003045"
     CDS             6454..7362
                     /locus_tag="IVY70_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP7960088.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7359..7901
                     /gene="sat4"
                     /locus_tag="IVY70_003046"
     CDS             7359..7901
                     /gene="sat4"
                     /locus_tag="IVY70_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAP7960089.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            7994..8788
                     /locus_tag="IVY70_003047"
                     /note="aph(3')-IIIa"
     CDS             7994..8788
                     /locus_tag="IVY70_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP7960090.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(8896..9084)
                     /locus_tag="IVY70_003048"
                     /pseudo
     CDS             complement(8896..9084)
                     /locus_tag="IVY70_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9064..9636
                     /locus_tag="IVY70_003049"
     CDS             9064..9636
                     /locus_tag="IVY70_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7960091.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
BASE COUNT         3492 a         1556 c         2171 g         2702 t
ORIGIN      
        1 ctgaacgaag tgagggaaag gctactaaaa cgtcgagggg cagtgagagc gaagcgaaca
       61 cttgatcttt taagttgcta tctttttaag gtcaatagag tatacttatt tgtcctaata
      121 tggttttagc agtataattg acttggtgaa taggtcattt aagttgggca taataggagg
      181 agtaaaatga aaaaatttat ttatcgagtt ttagaaaatg acgaagtggt ggctattttt
      241 aatgagcaac aatatgcgca agattttatc gcttacgaaa agacaatttc tgataagcaa
      301 tttgaaattg aaaaagtaga tatagctgat tggttattgc aaccgagaga attttagagg
      361 ttggttgaaa atggctaaaa ttggttatgc acgtgtcagt agcaaagaac agaacttaga
      421 tagacaactg aaagcgttag agggcgtttc taaggtcttt tcagacaaat taagcggtca
      481 atcggtcgaa cgcccacaat tacaagctat gcttaactat attcgtgaag gggatatagt
      541 tgttgttact gaattagatc gattaggacg aaataataaa gaattaacag aattgatgaa
      601 tcaaattcaa attaaggggg caaccctgga agtcttaaat ttaccctcaa tgaatggtat
      661 tgaagatgaa aatttaagac ggctgattaa taatttagtg attgaattgt ataagtacca
      721 agcggaatct gaacgcaaac gaattaaaga acgccaagcc caaggaattg aaattgctaa
      781 gaaaaaagga aaattcaaag ggcgacaacc taaattcaaa gaaaatgatc cacgtttaca
      841 acacgctttc gatttatttt tgaatggttg ttccgataaa gaagttgaag aacaaactgg
      901 aattaatcgc cgaacgttta gaaggtatcg atcaagatac aacgtgacag tagatcaaag
      961 aaaaaaacaa tgaaaagagg gatagttaat gagtacggtt attttagctg aaaaaccaag
     1021 ccaggcatta gcctacgcaa gtgctttaaa acaaagcacc aaaaaagacg gttattttga
     1081 gatcaaagac ccactattta cagatgaaac gtttatcacc tttggttttg ggcatttagt
     1141 cgaattagca gaaccaggtc attataacga aaagtggcag aactggaaac ttgaatcttt
     1201 gccgattttt cctgatcgat acgattttga agttgcaaaa gataagggaa agcagtttaa
     1261 aattgttgct gaacttctca aaaaggcaaa tacaattatt gtcgcaacag atagcgacag
     1321 agaaggtgaa aatatcgcct ggtcgattat ccataaagca aatgcctttt ctaaagataa
     1381 aacgtataaa agattatgga tcaatagctt agaaaaagat gtgatccgaa gcggttttca
     1441 aaatttgcaa ccaggaatga attactatcc cttttatcaa gaagcgcaaa cacgccaaat
     1501 tgccgattgg ttgatcggca tgaacgcaag ccctttatat accttgaatt tacaacaaaa
     1561 aggcgtacaa ggtacatttt cattaggacg tgtacaaaca ccgactttat atctcattta
     1621 tcaacgccag gaagcaatag aaaactttaa aaaagaacct tttttcctga ataattcata
     1681 gttttactaa tagtctttcc taacattgat ttaatagtct ttaaagtatt ctgcttcatc
     1741 acccattagg tgatgaaaaa agaacccctt tctgatttag cggacaaata ctttgaccta
     1801 atctcagaag atataaaaga aaaaataata aaatatccga tttatacttt gtataaagaa
     1861 ctaaagtagt tagcaaatca tttacagcaa atcactatgt tccacatacc attttacata
     1921 cttaccttat ccacgacaca tgttgagata gtatcgaaga taaagaaagt tgctccatgt
     1981 tgtttttgcg agatggggaa atcaaagcca cattgacaac catgatgaat aagcttgctt
     2041 tcagtcataa gctaatccta gaaccacttt tcaagtcagt tagccaaatc gatgaagaaa
     2101 gcgaccgtga aagaatggac gctattgata agctaatgga gcaactattg gaagaacgca
     2161 atacccttat tgcactgatg tccaaaggtt tcctagaacc tgctcttttt aatcaggaac
     2221 gaaatgtttt agatagtgag attaaaaatc ttactaccga aaaaacaaac ctggtaacga
     2281 actctgcgag tggggttttg cgagcaaacg agataaagga cctcattaat tatgtgtcag
     2341 cagataattt taatggtgac tacacggaag aactatttga agaatttgta gtgaacatca
     2401 ttgtaaattc cagggatgag ctgacattca atttgaaatg cggtctttcc ctgaaagaaa
     2461 aggtggtgag ataaatggca tatattccat atggatacaa aattcaagat ggagtggtta
     2521 ctgtcgatga aaaggcagca ggtcaagtaa aggtattctt tgagaaatac atatcaggac
     2581 tatcccttac agtggctggc gaacaggcag gtattgaaaa gacacattct gtgatggggc
     2641 gcattttgaa aaacgtcaac taccttggaa atgatacgta tccagcaatt attgataaag
     2701 agatatttga taaagctgaa gaagttagag ataaacgtgc aaaggattta ggacgagtgg
     2761 tagagcttgc cgctttcacc tctccccctc ccaaagaacg attcaaaatg aaaaaggcag
     2821 ataataagat gccagtggat ccttttgaac gagcagaata cttatacagt ctgatagaaa
     2881 gcgaggaata aagtgacaga gaaaaatata atggttattc ctgctcgtaa aagagtagga
     2941 agtacagccg caaaagaaaa gataaagaaa cttcgtgttg ctgcctattg ccgtgtttct
     3001 acagaaacag aagaacaaaa ttctagctat gaggttcagg tctcacacta tacagagttt
     3061 ataaagaaaa ataatgaatg ggagtttgct ggcatctttg cagatgatgg tatctctggt
     3121 acgaacacta aaaagcgtga cgaatttaat cgcatgattg cagagtgtat ggacggtaac
     3181 atcgacatgg ttattacaaa atccatcagc cgatttgcgc gtaacaccct agactgcctt
     3241 caatacatta gacagctcaa ggataagaac atatcagttt attttgagaa agagaacatc
     3301 aacaccacgg atgccaaagg tgaggttttg ctgactatta tggcatcttt ggcacagcag
     3361 gaaagccaga gcctttcaca aaacgttaag cttgggctac agtaccgata ccaacaagga
     3421 aaggtgcagg tcaaccataa gcgatttatg ggctacacga aagatgaaga tggaaattta
     3481 actattgtcc ccgaagaagc tgagattatc aaacgcatct accgagaata ccttgagggt
     3541 cagagtctag tgggcattgg tcgagccctt gaaaaggatg gtattttaac agcagcggga
     3601 aaaccaagat ggcgaccaga atcagttaag aagatacttc aaaacgaaaa atacatcgga
     3661 gatgcccttc tgcaaaagac tgtcacagta gattttctga ccaagaaacg agtgaaaaat
     3721 gaagggcatg ttccccaata ttatgttgaa aatagccatg aagcgattat tcctaaagag
     3781 ttatttttgc aggcgcaaga agaaattcat cggagaagca atatctacac aggagaaggc
     3841 aagaacaaaa gaatttacag tagcaagtac gctttaagtg ccatcacctt ctgtggagat
     3901 tgtggcgata tttatagacg ggtctattgg aatatccacg gaagaaaaga atttgtctgg
     3961 cgatgcgtga ctagaatcga gcaaggtcct gaagtttgta agaaccgaac cgtaaaagaa
     4021 gatgaacttt atggtgcagt aatgactgcg attaataagc tacttgcagg tgggaacaat
     4081 atgataaaga cactggaaga gaatattcat gctgtgattg gcgaaaccac agaataccaa
     4141 atttcagaga ttaacaactc actagaggaa aaacaaaaag aactaatcaa gctggcgaac
     4201 aagggtcaag actatgacca cttagcagat gagattgatg agctgagaga caagcgacag
     4261 atccttttag tagaagatgc ctccctcagt ggcgagaacg agcgaatcaa tgagctgatt
     4321 gaatttatcc gcaagaacaa attccgcacc ttagaatatg atgataagct tgtaaggaag
     4381 ataatccaga gcgttacagt ctatgaaggc cacttcgtca tagccttcaa atctggcatc
     4441 gaaatggaaa tatgaaaacc agaagtaaat agcccatgac tctacagtag agttgtgggt
     4501 tttcttttgc ttgctgatta taataatatt ttaataacta ttgtgtttat caaccaaatg
     4561 gtttataata ttcttagcgt aaacttggag gtgctgtatg actacggcag aaatgattaa
     4621 agaactgtgt gagcaaatga atataagtgt ttccgaactt gctagacgta ttggccagac
     4681 tccacagaat ttcaataaaa aattacaacg agaaacagta accttggatg agttgaaggc
     4741 cattgctgat gtgctaggtg tcaagtttgt gcaggcattt attttaccag atggagaaga
     4801 aataaaaata tctaacgaat aaaggaggat gtttatatgg taggtaatgt tattcaaata
     4861 gtaacagaga aattatcatc tttgcctttt atagaaggca tcgtattagg gggctcacgt
     4921 gcaagaggca cccatacaga aaattctgat atagatatcg gaatctatta caattcagac
     4981 tcatttgacc tgacagcgat taatcaaatt gctacagagt tggatgatga gaatagaaac
     5041 aaccttgttg tacctcccgg agcgtggggt gattgggtta atggcggtgg atggttagtt
     5101 ataaacgggt gtcatgttga cttgatatta cgtgatataa aacgggtgga acaaataatc
     5161 aaagatacgg agcaaggaat tgttactgcc aattatcaga ccgggcatcc ccatggttat
     5221 ataagtgcta tgtatcgtgg agaattagcg attagtaaaa tactatatgc taagaacgaa
     5281 agcttatgcg aattaaaaaa gcaggcagaa atttacccta ctgctctaaa gaaaagcttg
     5341 atgaactttt ttatatttga agctgaattc tctttaatgt ttgtaaaagc aaatgcgggg
     5401 gcagaggata aatattatat tgcaggccat gtttttcgta ttatttcatg cttaaatcaa
     5461 gtactatttg catgtaataa tgcttattgc attaacgaaa agaaagctat aaaactgctt
     5521 gaaacttttg aatataaacc tgaaaaatat gccgaaagag ttaatcatat ttttgaagta
     5581 ctcggtcttt ctctttttga atgctacgac atgaccgaga aactttataa agaagtgaat
     5641 gaaattgtat cggagataaa taacttttta aacgaggaga gttcagatga aagaaaacaa
     5701 atatgatgat aatatatttt ttcaaaaata cagtcaaatg agtcgctcgc agaaaggact
     5761 ggctggtgcg ggagaatggg agactttgaa aaagatgcta cctgatttta agggtaagcg
     5821 tgtgcttgat ttaggatgcg gctatggatg gcactgtata tatgcgatgg aaaacggtgc
     5881 ttcctctgta gtaggtgttg atatttctca taaaatgctc gaagtagcaa aaggaaaaac
     5941 ccattttcca cagattgaat atgaatgctg tgccatagaa gatgtggatt tcccagagga
     6001 gagctttgat gtaatactaa gttcgcttgc gtttcattat gtagcagact atgagaattt
     6061 aataaaaaag atatatagga tgctgaaggc tggtggcaat ttagttttta cagttgaaca
     6121 tcctgttttt actgctcatg gaacacaaga ctggtattat aacgaaaaag gagaaatact
     6181 gcatttcccg gtggacaatt attattatga gggcaaacgg acagctatgt ttttggaaga
     6241 aaaggttaca aaatatcata gaacactgac cacatatcta aatacactgc tttcaaatag
     6301 ttttataata aatcagattg tggagccaca gccgccagag aacatgatgg atattccggg
     6361 ggtggcggat gaaatgcgac gcccaatgat gctgattgta tcggcaaaaa agaagatgta
     6421 ataatataga aaaaataaac gaggagtatg taaatgagat cagaaaaaga aatgatggat
     6481 ttagtacttt ctttagcaga acaggatgaa cgtattcgaa ttgtgaccct tgaggggtca
     6541 cgcgcaaata ttaatatacc taaagatgaa tttcaggatt atgatattac atattttgta
     6601 agtgatatag aaccgtttat atctaatgat gactggctta atcaatttgg gaatataata
     6661 atgatgcaaa agccggagga tatggaatta ttcccacctg aagaaaaggg attttcctat
     6721 cttatgctat ttgatgatta caataaaatt gatcttacct tattgccctt ggaagagtta
     6781 gataattacc taaagggcga taaattaata aaggttctaa ttgataaaga ttgtagaatt
     6841 aaaagggaca tagttccgac tgatatagat tatcatgtaa gaaagccaag cgcaagggag
     6901 tatgatgatt gctgcaatga attttggaat gtaacacctt atgttattaa aggattgtgc
     6961 cgtaaggaaa ttttatttgc tattgatcat tttaatcaga ttgttcgcca tgagctgctg
     7021 agaatgatat catggaaggt cggcatcgaa acaggcttta aattaagtgt aggcaagaac
     7081 tataagttta ttgaaaggta tatatccgag gatttgtggg agaaactttt gtccacctac
     7141 cggatggatt cctatgaaaa catatgggaa gcattatttc tatgccatca attgttcagg
     7201 gcggtatccg gtgaggtggc ggaaaggctt cattatgcct atccggagta tgataggaat
     7261 ataacaaaat ataccaggga catgtataaa aaatacactg gtaaaaccgg ctgcctggat
     7321 agcacatatg ccgctgatat agaagagagg cgggaacagt gattacagaa atgaaagcag
     7381 ggcacctgaa agatatcgat aaacccagcg aaccatttga ggtgataggt aagattatac
     7441 cgaggtatga aaacgagaat tggaccttta cagaattgct ctatgaagcg ccatatttaa
     7501 aaagctacca agacgaagag gatgaagagg atgaggaggc agattgcctt gaatatattg
     7561 acaatactga taagataata tatctttact accaagacga taaatgcgtc ggaaaagtta
     7621 aactgcgaaa aaattggaac cggtacgctt atatagaaga tatcgccgta tgtaaggatt
     7681 tcagggggca aggcataggc agcgcgctta tcaatatatc tatagaatgg gcaaagcata
     7741 aaaacttgca tggactaatg cttgaaaccc aggacaataa ccttatagct tgtaaattct
     7801 atcataattg tggtttcaaa atcggctccg tcgatactat gttatacgcc aactttgaaa
     7861 acaactttga aaaagctgtt ttctggtatt taaggtttta gaatgcaagg aacagtgaat
     7921 tggagttcgt cttgttataa ttagcttctt ggggtatctt taaatactgt agaaaagagg
     7981 aaggaaataa taaatggcta aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa
     8041 ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata agctggtggg
     8101 agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga ccacctatga
     8161 tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg ttccaaaggt
     8221 cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg ccgatggcgt
     8281 cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg agctgtatgc
     8341 ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata cgaatagctt
     8401 agacagccgc ttagccgaat tggattactt actgaataac gatctggccg atgtggattg
     8461 cgaaaactgg gaagaagaca ctccatttaa agatccgcgc gagctgtatg attttttaaa
     8521 gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag acagcaacat
     8581 ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg gcagggcgga
     8641 caagtggtat gacattgcct tctgcgtccg gtcgatcagg gaggatatcg gggaagaaca
     8701 gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga aaataaaata
     8761 ttatatttta ctggatgaat tgttttagta cctagattta gatgtctaaa aagctttaac
     8821 tacaagcttt ttagacatct aatcttttct gaagtacatc cgcaactgtc catactctga
     8881 tgttttatat cttttctaaa agttcgctag atagagttct atattagaga tgtaaagagt
     8941 tttggtgaag aggtttgtga gtatggcttt tataaccagg gtgcaatgag aagcactaac
     9001 agcagctagc ttaagaacgc ataggatata ctttttatgg aagtcacaga ggaggaataa
     9061 aatttgaaca ggattaacag agtaacctct attcttattc agctgcaatc aaaaaaaata
     9121 attcctgcca aagaaattgc acagcgattt aatataagct taaggacagt ttacagagat
     9181 atccggacac ttgaagaagc cggaatacca attggatctg aggccggaaa aggatatttt
     9241 cttgtagagg gcttcctact tccgccggta atgtttacag cggcggaagt gggtgcattg
     9301 attacagcag ggaaattttt aaattgccat ggagatgaat catttataaa ggattttgat
     9361 tcagctatgt ataaaatcaa atctattttg aagcatggtg aaaaaaacta tgcacaggag
     9421 ttggagaaca gtattaacgt gtacagcaca tctggacaga aaaatacatt ggcggataac
     9481 gttattgcag caattcagac tgcaatctgc aataaaaggg taatatcaat tcaatatcct
     9541 gcatcaggag ggcaagaacc ggaaagcaga atgatagagc ctatatcact ggggttttat
     9601 gaacagaact ggtacttaat cggttttgca ggctaagaaa tgaatatagg aattttcgtg
     9661 tggatcggat taaaagcatt tgcatagaag aagagctctg tcaaagccat gatggctctt
     9721 tggaacaaat attaaaacaa atgctttcat ataaaaaact atatgcccta caatgccgtt
     9781 gtaaaaattg gggaaaaggg atatattgat tttatatctt tctcaaaata aatataaaag
     9841 ccctttaaag agggctttta tatattaatc acaaatcact tatcacaaat cacaagtgat
     9901 taatcacaaa tcacttgtga t
//
LOCUS       DACCXO010000307         9425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.guided.106, whole genome shotgun sequence.
ACCESSION   DACCXO010000307 DACCXO010000000
VERSION     DACCXO010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.guided.106"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..1599
                     /locus_tag="IVY70_003050"
     CDS             <1..1599
                     /locus_tag="IVY70_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAP7960092.1"
                     /translation="AAAAGMTVEEYAAAGYEAPQQDTFSIYQLRGGEETRDYRFEPYE
                     RLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNIDHPADFRGHSLSVSDVVVLHQ
                     NGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNENMIDGIINNTPPEPQPEEPEQ
                     LSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAARRAKEAMSFSDYRPGSATAEY
                     RHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLAENMNKGYEITARVPSVLIAG
                     PSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGMGGISADDPQAVSKLEKKLEK
                     LEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKADMASGWHYEKKPFQSWQLSNN
                     NAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVANRLQIFFEGKPDAAVRDELK
                     SNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEKPSALQLAARREKEKPSIRAQ
                     LNAAKTEQAKQPPAREKSKGLEVE"
     gene            1600..2544
                     /locus_tag="IVY70_003051"
     CDS             1600..2544
                     /locus_tag="IVY70_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7960093.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            2548..2751
                     /locus_tag="IVY70_003052"
     CDS             2548..2751
                     /locus_tag="IVY70_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7960094.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(3051..3287)
                     /locus_tag="IVY70_003053"
     CDS             complement(3051..3287)
                     /locus_tag="IVY70_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7960095.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(3470..3841)
                     /locus_tag="IVY70_003054"
     CDS             complement(3470..3841)
                     /locus_tag="IVY70_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7960096.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(3838..5166)
                     /locus_tag="IVY70_003055"
     CDS             complement(3838..5166)
                     /locus_tag="IVY70_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7960097.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(5127..5456)
                     /locus_tag="IVY70_003056"
     CDS             complement(5127..5456)
                     /locus_tag="IVY70_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP7960098.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(5714..6084)
                     /locus_tag="IVY70_003057"
                     /pseudo
     CDS             complement(5714..6084)
                     /locus_tag="IVY70_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            6229..6414
                     /locus_tag="IVY70_003058"
     CDS             6229..6414
                     /locus_tag="IVY70_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7960099.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            6926..7144
                     /locus_tag="IVY70_003059"
     CDS             6926..7144
                     /locus_tag="IVY70_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7960100.1"
                     /translation="MRQERPEGVHTGPACVKSHTARCIRKKEAAHFAEAWGVPNKQNE
                     NAAGGQVVDQNIRKGYSDPLLASIIGMF"
     gene            7357..8016
                     /locus_tag="IVY70_003060"
     CDS             7357..8016
                     /locus_tag="IVY70_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HAP7960101.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            8019..9362
                     /gene="vanS"
                     /locus_tag="IVY70_003061"
     CDS             8019..9362
                     /gene="vanS"
                     /locus_tag="IVY70_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP7960102.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
BASE COUNT         2341 a         2354 c         2468 g         2262 t
ORIGIN      
        1 gccgccgcag ccggtatgac cgtggaggaa tacgcggcgg ctggctatga agcaccccaa
       61 caggacacct tttccatcta ccagcttcga ggcggggagg aaacccgcga ctatcggttt
      121 gagccatacg agcgtttaca ggccgcaggg ctggccgtgg acagtgccaa ctatgacctt
      181 gtttatacgg caccgctgga taaaagcacg tctttggagg atatttaccg cacattcaac
      241 atcgaccacc cggcagattt taggggacac agcctttccg tttccgacgt ggtggtgctg
      301 caccagaatg gacaggacac cgcccactat gtagacagct tcggctatat ggaagtgccg
      361 gagtttttgc gggaaaatcc cctgcgtacc gccgaactgt ctacggagca gaacgaaaac
      421 atgattgacg gcattatcaa caatacgccg cccgaaccgc agccggagga acccgaacag
      481 ctatcttttg aggatgtggc agttgcttcc ggccagacac aggaggaacc cccggaggct
      541 gcgcccccgc aaacggctgt ccgctattat cccatcaatg agggagccgc ccgccgcgcc
      601 aaggaagcca tgagcttttc tgactaccgg cccggcagcg ccacggcgga ataccggcac
      661 tatgtcgatc aggccgtgga aattgcggag cgccagaaaa agcgtgtgga gccagagtat
      721 cacgaaaaga tagacagcct ccttgacacc tacgcccgca agctggccga gaacatgaat
      781 aagggctatg agattaccgc ccgcgtcccc tctgtcctga ttgccgggcc gtccaatttc
      841 ccggtacggg cgaaagaaaa gcagaacgcc gccagtgacc gcaacatgga ggaatttcag
      901 tacattcagg gcttgcttga caaaatccgc agcaccggca tgggtggtat cagcgccgac
      961 gatccgcagg ccgtttccaa gctggaaaag aaactggaaa agctggaagc gtcgcaggag
     1021 ttgatgaagg ccgtcaatgc ctactaccgc aagcatggga cgctggacgg atgcccccat
     1081 ctgatcgaaa gaggcattga aaacctgaaa gcggatatgg cgtcgggctg gcactatgag
     1141 aaaaaaccgt tccagtcctg gcagctctcc aacaataacg ccgagatacg ccggttaaag
     1201 ggccgcattg aggaattgac ccgccaaaag gaggcggcct atgtgggatg ggagtttgac
     1261 ggcggcactg tggagatcaa ccgggtggcc aaccgcctgc aaatcttctt tgaggggaag
     1321 ccggatgctg ctgtacgcga cgagctgaaa agcaacggtt tccgctggtc gcccaaggcc
     1381 ggggcatggc agcggcagtt aaacgacacc acaatccgcg tcgctgaccg tatcaagtgc
     1441 atccagcccc tttccggcga aaagcccagc gccttacagc ttgcggcccg cagggagaaa
     1501 gaaaagccct ctatccgggc gcagctcaac gcggccaaga ccgaacaggc caagcagcca
     1561 ccggcgcggg aaaaatcaaa gggattggag gtagagtaaa tgcagaagtt tgaaaatgtg
     1621 gacgttctgg ccgcgctgga acagatcatg cggcagaaca ccgcgtttta tcaaagtgac
     1681 tttggcattg acaaagacat tatcaggcgg gatgccgcca gcgatcaggc cgcagacaag
     1741 accctgctct ggatgtcccg gccctccggg acgtactgtt tccgggaacg tgatgttttc
     1801 ctgaaagaca cccgacagta caacacatgg aaattttacg gggagcagac ccgtgacaag
     1861 atactggcct acgcggtgga gctgaccggc acacagggcg gcactatccg gggcaatctc
     1921 tatgagctgg actatccgca gcatttccgg cacgtcattg aaaccgccca gccggtgagt
     1981 gtgaaccggc tcttttatga gcatggcacc cgtgacatcc cggagagcca gcgttttgac
     2041 ggaagccctg accgggtatt gggaaacttt ttgtactatg aggcccagcc ccatgacccg
     2101 gctgtgctgc aggaggcgct acggacggag cagcacagcc gggggcgggc cgtccccggc
     2161 gatttcaaag tccacgtcac cgctttgcat gacagccgga ttgaaaccga ggcccggcgt
     2221 attgtggaca gcctgaaaga tctgtccgcg cctaacagcc ccaataaaac gcatttcatg
     2281 gtggagatct ccccgcactt tgcggcgatt gcttccacca aggataatga ccggctcttt
     2341 gccatgctgc cctataaatc cctgtgtttt accgggatga aaggcaggca tggcgtgtat
     2401 gccgtgatta accgggacga gcgccgggat gtgagtattc gcaggccccg gccctctatc
     2461 cgcaagcagc tatccgacag caaacaggcc gccagcccaa aaaaggcggc ggccagaacc
     2521 aaaaagaatg aattggaggt ataaggaatg atgcacttta ccgtggagga agaaaacctg
     2581 atctgtatgt accacaatgc agaccggcgc agaacgataa acaggctcac cgccgtcatg
     2641 ccggttatgg atggggatat gcgggcgcag gcccagcaga ccctttccaa gctggaacgc
     2701 atgacggatg ccgactatga cggccagaag ttccacttca cagacgaata acgaacagca
     2761 gacgccgggc gcggcggcca tgtggtcgct tgcgtccggc gtcttttctt tttgcctatt
     2821 cctacgcatt tagaacccgg tattgtcggg gataggatga agtattcgct tgcccctgca
     2881 aacatgcggg agaagccttg cagcgggcgg ttttcaatcc cttaaatgcg taggttgcag
     2941 ctataattag acccctgaaa cagatatagc cgcgcagcgg gtgtatctgt ttcaggggtg
     3001 tgcaggtgat agccgcaaag cggcgtaaga gggtgcagcc ctcttgccgt ctatctctgt
     3061 tccagcggga agctcggcaa tgcttccagc agtttcaggg aaatgcgctg ccgtatatct
     3121 tcgtcaatga ctttcttctt tttgccgttt gcctgtctga tctccaccgt ggaaagctcg
     3181 ttgatgtagt cagcataatg ggccaacact ttttccgtgg cccacctctc accggcaacg
     3241 gcggctttga tcgtttcata gtccagcagc ttttcttgtc cgtccatcct gatacccccg
     3301 tcagactgat tttttctatg ataccatatt cggaagcatg gaaccagaga aaacggcgct
     3361 tttctgctgg taaattctac gcatttagaa cagcacttcg gcggggatag gataaaacac
     3421 ccacataccc cgctgaccat gccggaaaag ccttgaaata taagggcttt tacagctcta
     3481 agtgcgtagg tttccagtat cttttccgct ggcgctcggc ctctttgcgg cggcgcatcc
     3541 gtttggagca ggccgcgcag tatttggccc ggttagactt cggcgtgaac gtggccccgc
     3601 actcacagca gcgtttcact tcgtcccggt ctttggtgat ctcggcatac agggccgcat
     3661 ccagcgggag gacagcggcc ttaaaccagc ggcacagaag cgaataggaa atgctctgta
     3721 cgcaaataca ttcttcgccg tcgtccagca acaggcagtt gccgtcacaa tagttgcagc
     3781 actcatgcgt cagcttccgg gccttgcgat attgagggta attcattcgt ggaatactca
     3841 tagctcttgc gtccattcct ttcccggcgt catacgcaaa atgccgtcca cattctgctt
     3901 gatgatgccg tattcctgca tctgcttttt cgcttcgccg tacttctcat acagccgttc
     3961 cttttcctcg gccaatcgct tgtactcggc tttcagggcg tacagatcgg ggagcttttt
     4021 cacaccctgt ccctttaggg cctttgccgc agcttcatac aaaatcaacg tgctttcatg
     4081 ctcacgccgg aattttgcct tatccgctgc attgcggtat tccatagcca cgggccagag
     4141 ctgcttgtag gtagaaatat tctttatcag caccgccata tccgcaagcc ggtgttccat
     4201 agctttgagg gtagcggcgg cctcgtcgct ggcggcggtg atctcggcca ctttgctttc
     4261 caaatccggg taggtgtcta tcccatgttc agtcaggaaa ttcaaagtct ttgcggcctg
     4321 cttcaaattg tggagcttcg cccacctctc atagcctgcc gactgctggg ccttaatgtt
     4381 gttttccaga tcaatccgca aagaaatccc cttgcgctct ttgggcgctc tggtgataac
     4441 gcggcctttg atacgctctt tgatggcttc ctcggtgtag gcttccccca atgtcttgca
     4501 gcgggtaaag cgttcctgtc cgggagcgcg gaaagaaata aactttcccg gcttgatctc
     4561 atagccctgc gcctgcatga gccgcaggaa atcatcaaaa tcttttgcct gcgggatagc
     4621 ggcgtcaatg gctgctttga gcttcgcttt ccagctcgtc cctttccgct gggcgtccca
     4681 ctcggcatag ctcttgccct tactctggcc gggcttcacc acggacaatc cgttttcctt
     4741 gcacagcctg tcgctggtgc gccggatata ggcatagctc tgtttgttgg aaatgtactt
     4801 gcgctgattt accatatcca cggcacagaa aataatgtga ttgtggacgt ggcccttatc
     4861 aatatgggta gtcagcacat agggatattt cccttgcagc acttcctcgg caagctgctt
     4921 cccgatctca tgggcctgtt ccggcgtggc ttcgccgggg gcaaaggatt gtatcaggtg
     4981 gtggcccaga ttattgccct tttggtatgc ctgcgccagc agcatttcaa attcaatgtc
     5041 cgctgtttca taagagcagg caaaggaggc caccagcagc ttgccgtcag ttttgtccgg
     5101 gttctcgatg tagtcaaggg cttttttcag cgttgacttg ataggatgta tctttgtaac
     5161 tgccatatct gcgccagcgc cccctttata tcctcgatgt cctgtgcgta caccggcccc
     5221 ataccgttca gcctctttgc aatctgattg acgttgacgc cgatcttctg tatctcggcg
     5281 gtctgcgccc ggatgtcgct ggtgtccatg ttgacgatat agccgtcaat gagcatcttc
     5341 cgggcgtagg ctgaaaagtt cttcgtatgc agcagcgcca ttttctgttc aatcatagcc
     5401 cgttcctccg gcgtcacagg gacgcgcagc acgatttttc gctttctgtt tgccatgctt
     5461 caccgtcctt tccattcagg ggtttgggga tttccccaac aagcaaaacg gacgcggccc
     5521 ggcagggacg gggcaggccg tttttccggg agtggctaca ctccctatgc ttgctacgtt
     5581 actgtttctc cactaacaag acgtttccaa agccccaaac tacccgcaaa atagccctct
     5641 ggaaagaaaa aagtgcaaaa aaatagaagc tacgctgtgc agctcccgct aaaagtaaaa
     5701 cagaggatgc cagttacaca tcctccgttt cattcttctt cggcttttca attagtatgg
     5761 cgctgtcaat cgcgccctcg atgatcggca ggtattcttc ggggcagagt ttcagcttgt
     5821 ggctgaccct ttgccgcagt tcgctttcct cccgcatgat ctccgggtta aagtagcgtt
     5881 ccacaggaag cccgcaggct ttaataagct gtatgacaac cggcagactg ggaatggccc
     5941 gtcgttttcg atgttggcaa gatagcgcca gtcaatattt attttctctg ccattgcttt
     6001 gcgcgaaacg cgctttgctt gccgggccgc tttcacatcc gcgccgaaag tttcaaagcc
     6061 gggacaatct tcaatattcg ccatatcgca cctcccactt acattgtata tttcatactc
     6121 tgcccgtgga atgttggtat atgcaccttt cttaaagttt atatttcaca ttttgtttct
     6181 tttattcttc gagcaaaaga attataatgt accctgtgga ggtgtactat ggactacatg
     6241 acgttgaaag aggcaggcga gaaatggggt gtgcccccac gatggataaa ctactattgt
     6301 gctgcgggac gcatccccgg cgctgtaaag atggcgacga tctggctcat tcccaaaact
     6361 gcggaaaagc cgattgacag gcgtacaaag aaaggaaagg cggttactga atagtataat
     6421 gaaaccggcg cattttggat atggttttga agagagagaa agcatggtat tttgtcacgg
     6481 aaaaactata agatgataga tggcaggctg ctccagaccg ataaaaagta ttctcatctg
     6541 aaacagaaag aaaagattta cacatggatg tatgaagaag cgaaacttta tcatgacaga
     6601 aatggaaaat gcccgaaaca aagacgagga agctgctgct gtatcagccg tatatggtag
     6661 aattgaaaag gccggcatct ggattcttta tggtgaagtc tccagacatt ggataactgc
     6721 tggtctaatg cgcctttgag tatggggtga ttgattttaa taccggggag gtacggcttt
     6781 ttgaggaaaa tattctggga tttacatttg gcgattatta catttcctgt aactgcaagg
     6841 acacggatga tgaagatatg aggaatatag acttgctgat tttctactgt cttgaacggt
     6901 attaggactg aatctgctgc gcggagtgcg gcaagagcga ccggagggag tacacacagg
     6961 ccctgcctgt gtgaaaagcc ataccgcaag gtgcattcgc aaaaaagagg ccgcgcattt
     7021 tgctgaggct tggggtgtcc ccaacaagca aaacgagaac gccgcaggcg ggcaagtggt
     7081 ggatcagaat atccgtaaag gatattctga tccattgctt gccagtatta tcgggatgtt
     7141 ttgacaaccg ctgtaatcga aacatcccaa acattaagaa ggtgttaatt attattatct
     7201 tgatttaatt cgtttaaacc aataatatag atacatacac catgaaaaga agcgagtatc
     7261 ccaatgacgg atgattaaaa tccgaaaaga aatcgctttg aaactacagg gaaactacag
     7321 actgttatgt tatcttctta aaatggaggg atttttatgt cgatacgaat tctacttgtc
     7381 gaggatgatg atcatatctg taatacagta agggcgtttc tggctgaggc aggatatcag
     7441 gtggatgcct gcacagatgg aaatgaggca tacaccaagt tttacgaaaa cacttatcaa
     7501 ctggttattc ttgatattat gctgcccggt atgaacgggc atgaactttt gcgtgaattt
     7561 cgtgcgaaaa atgatactcc cattctgatg atgacagccc tgtcggatga cgaaaaccaa
     7621 atccgggcgt ttgatgcaga ggcagacgac tatgtaacaa agccattcaa gatgcagatt
     7681 ttactaaagc gggtggaagc cctgctgcgg cgcagcggtg cgctggcaaa ggaaatccgt
     7741 gtcggcaggc tgacacttct gccggaggat ttcacggtac tttgtgacgg tacagagctg
     7801 cccctgacac gaaaagaatt tgaaatcctt ttgctgctgg tgcagaacaa aggcagaacc
     7861 ttaacgcatg aaatcatttt gtcacgtata tggggatatg actttgaggg tgatggcagc
     7921 acagtccaca ctcatatcaa aaatttgcgg gccaagctgc cggaaaatat catcaaaacc
     7981 attcgcggtg taggataccg attggaggaa tcataataat ggaaagaaaa gggattttca
     8041 ttaaggtttt ttcctatacg atcattgtcc tgctactgct tgtcggtgta acagcaacac
     8101 tgtttgcaca gcaatttgtg tcttatttca gagtgatgga attacagcaa acagtaaaat
     8161 cctatcagcc attggtggaa ctgattcaga atagcgatag gcttgatatt caagaggtgg
     8221 cagggctgtt tcactacaat aaccaatcct ttgagtttta tattgaagat aaagagggaa
     8281 gcgtactcta tgccacacca aatgccaata catcaaatag ttttagaccc aactttcttt
     8341 atgtggtaca tagagatgat aatatttcga ttgttgctca aagcaaggca ggtgtgggat
     8401 tgctttatca agggctgaca attcggggaa ttgttatgat tgcgataatg gttgtattca
     8461 gccttttatg cgcgtatatc tttgcgcggc aaatgacaac gccgatcaaa gccttagcgg
     8521 acagtgcgaa taaaatggca aacctgaaag atgtaccgcc gccgctggag cgaaaggatg
     8581 agcttggcgc actggctcat gatatgcatt ccatgtatgt caggctgaaa gaaaccatcg
     8641 caaggctgga ggatgaaatc gcaagggaac atgagttgga ggaaacacag cgatatttct
     8701 ttgcggcagc ttctcatgag ctaaaaacgc ccatcgcggc tacaagcgtt ctgttggagg
     8761 gaatgcttga aaatatcggt gactacaaag atcattctaa gtatctgcgc gaatgcatca
     8821 aaatgatgga taggcagggc aaaatcattt ccgaaatact ggagcttgtc agcctgaatg
     8881 atgggagaat cgtacccata gctgaaccgt tggacatagg gcgcacggtt gccgagttgc
     8941 tgcccgattt tcaaaccttg gcagaggcaa acaaccagcg gttcgtcaca gatattccag
     9001 ccgggcaaat tgtcctgtcc gatccgaggc tgctccaaaa ggcactatcc aatgtcatat
     9061 tgaatgcagt tcagaacacg ccgcagggag gcgaggtacg gatatggagt gagcctggtg
     9121 ctgaaaaatg ccgccttttt gttttgaaca tgggcgttca cattgatgat actgcgcttc
     9181 caaggctgtt caccccattc tatcgcattg atcaggcgcg aagcagaaaa agtgggcgaa
     9241 gcggtttagg acttgccatc gtacaaaaaa cgctggatgc catgagcctc cagtatgcgc
     9301 tggaaaacac ctcggatggc gttttgttct ggctggattt accgctcaca tcaacattgt
     9361 aaatatttaa aacttaaatg attttgggct ctttgtcaaa taggactgat agagtgagat
     9421 aagaa
//
LOCUS       DACCXO010000308         8078 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.guided.126, whole genome shotgun sequence.
ACCESSION   DACCXO010000308 DACCXO010000000
VERSION     DACCXO010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.guided.126"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(134..577)
                     /locus_tag="IVY70_003062"
     CDS             complement(134..577)
                     /locus_tag="IVY70_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP7960103.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="IVY70_003063"
     CDS             complement(795..2135)
                     /locus_tag="IVY70_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7960104.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="IVY70_003064"
     CDS             2404..3300
                     /locus_tag="IVY70_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP7960105.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="IVY70_003065"
     CDS             complement(3450..4391)
                     /locus_tag="IVY70_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP7960106.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="IVY70_003066"
     CDS             4517..5638
                     /locus_tag="IVY70_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7960107.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /locus_tag="IVY70_003067"
     CDS             5896..7398
                     /locus_tag="IVY70_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP7960108.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="IVY70_003068"
     CDS             7465..7992
                     /locus_tag="IVY70_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7960109.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         2675 a         1507 c         1599 g         2297 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttg
//
LOCUS       DACCXO010000309         7959 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.guided.129, whole genome shotgun sequence.
ACCESSION   DACCXO010000309 DACCXO010000000
VERSION     DACCXO010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.guided.129"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            128..1564
                     /locus_tag="IVY70_003069"
     CDS             128..1564
                     /locus_tag="IVY70_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7960110.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="IVY70_003070"
     CDS             1753..4200
                     /locus_tag="IVY70_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP7960111.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTELEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="IVY70_003071"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="IVY70_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP7960112.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="IVY70_003072"
     CDS             complement(5690..6793)
                     /locus_tag="IVY70_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7960113.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="IVY70_003073"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="IVY70_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP7960114.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2665 a         1358 c         1729 g         2207 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagct ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattactt
//
LOCUS       DACCXO010000310         7819 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.guided.131, whole genome shotgun sequence.
ACCESSION   DACCXO010000310 DACCXO010000000
VERSION     DACCXO010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.guided.131"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            194..1000
                     /locus_tag="IVY70_003074"
     CDS             194..1000
                     /locus_tag="IVY70_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HAP7960115.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            1018..1845
                     /gene="vanW"
                     /locus_tag="IVY70_003075"
     CDS             1018..1845
                     /gene="vanW"
                     /locus_tag="IVY70_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAP7960116.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVFADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            1842..2813
                     /locus_tag="IVY70_003076"
     CDS             1842..2813
                     /locus_tag="IVY70_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP7960117.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2806..3834
                     /gene="vanB"
                     /locus_tag="IVY70_003077"
     CDS             2806..3834
                     /gene="vanB"
                     /locus_tag="IVY70_003077"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP7960118.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            3840..4448
                     /locus_tag="IVY70_003078"
     CDS             3840..4448
                     /locus_tag="IVY70_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP7960119.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            5036..5467
                     /locus_tag="IVY70_003079"
     CDS             5036..5467
                     /locus_tag="IVY70_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7960120.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            5474..5707
                     /locus_tag="IVY70_003080"
     CDS             5474..5707
                     /locus_tag="IVY70_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7960121.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(5629..5871)
                     /locus_tag="IVY70_003081"
     CDS             complement(5629..5871)
                     /locus_tag="IVY70_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7960122.1"
                     /translation="MAHQPSGSAQTECVRMPYDAPPLSCGHIYQGAIRPPGKHRNGER
                     VSRFMLPGPVRQFVTIAWMSLISRRRFMASSRHTCG"
     gene            6128..6331
                     /locus_tag="IVY70_003082"
     CDS             6128..6331
                     /locus_tag="IVY70_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP7960123.1"
                     /translation="MMNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVI
                     MNGNRIQIKRKQFEKIIDTLDTI"
     gene            6415..7608
                     /locus_tag="IVY70_003083"
     CDS             6415..7608
                     /locus_tag="IVY70_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7960124.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
BASE COUNT         2201 a         1711 c         2075 g         1832 t
ORIGIN      
        1 gatagaatga ttttctatca gtcctagaaa ttatagagcc gattttgacc gacaggtata
       61 accctgccgg tctttttttg ctgctacagg aaaactacag actggctaca gggaaagtac
      121 agatgcgctt gccataataa caatcgtacc agccacaaat cgtagtttta ttgcaaagga
      181 ggcattcgat caaatggaaa aaagcaacta tcattccaat gcggatcatc acaaacggca
      241 tatgaaacaa tccgtggaaa agcgggcttt tctatgtgcg ttcattattt cgttcacagt
      301 ctgcacgctg tttttggggt ggagactggc ttccgtattg gaggcaacac agataccgcc
      361 catccctgca actcatacag gcagcagcac tgacgtagtg gagaatttgg aggaaaacgc
      421 tcttgccacc gccaaagaac agggagatga acaggaatgg agcctgattt tagtgaacag
      481 gcagaacccc atccccgcac agtacgatgt ggagcttgag caactatcaa atggtgagcg
      541 gatagatatt cggatttctc cctatcttca agatttgttt gatgccgcaa gaactgatgg
      601 agtttacccg attgtcgcat ccggataccg aacaacagaa aaacagcaag aaattatgga
      661 tgaaaaaatt gccgaatata aggcgaaagg ctacacctct gcacaggcta aagcggaagc
      721 agaaacttgg gtggccgtgc cgggaacgag cgagcatcag cttggtcttg ctgtggatat
      781 caatgcggac ggaattcatt caacaggcaa cgaggtttat agatggctgg atgaaaacag
      841 ctatcgtttt ggttttattc gtcgctaccc accagacaag acagagataa ccggtgtgag
      901 caacgagccg tggcattacc gatatgttgg catcgaagct gccacagaga tgtacaacca
      961 aggggtttgc cttgaggaat atttaaaacc agaaaaatga gaaaaggatg ttatgttatg
     1021 gacagaaaaa gattgacaca gcgtttcccg ttcctgcttc caatgagacg ggcgcagaga
     1081 aaaatgtgct tttatgcggg aatgagattt gacggctgtc gctatgcgca gacgataggg
     1141 gaaaaatcgc tttcccattt gctctttgaa acggattgcg cattatacaa ccacaatacc
     1201 ggatttgaca tgatatacca ggaaaacaag gtgttcaact taaagctggc agcaaagacc
     1261 ttaaacggct tattgataag accgggggaa accttttctt tctggtggct ggtacgccat
     1321 gcggacaaag atacccccta taaagacggc cttacggtga ccaatggtaa actcaccacc
     1381 atgtcgggcg gcggtatgtg ccagatgagc aatttactat tttggatgtt cctgcatacg
     1441 ccattgacaa ttatccagcg cagaggtcac gaagtaaagg agtttccaga gccaaacagt
     1501 gacgagatta aaggggtgga tgcaaccatt tcagagggct ggattgattt aaaagtgcga
     1561 aacgataccg actgcaccta ccaaatatgg gtgaccttag atgatgagaa aatcatcggt
     1621 caggtgttcg ccgacaaaga gccccaagca ttatacaaaa ttacaaatgg cagtatccag
     1681 tatgtccgtg aaagtggcgg gatttatgaa tatgcccagg ttaaacggat gcaagttgcc
     1741 ttaggtaccg gggaaataat agattgcaag ctgctttata caaacaaatg caaaatctgt
     1801 tatcccctcc cggaaagtgt ggatattcag gaggagaacc aatgagaaaa agtgtgggca
     1861 ttactgtttt tggatgcgaa caggatgagg caaatgcttt ccgcgcttta tcgccggatt
     1921 ttcatattat ccctacgctg attagcgatg cgatatcggc agacaacgca aaattggccg
     1981 ctggcaatca atgcgttagc gtaagccata agtccgaggt ttccgaggcg acaattcttg
     2041 cgctgagaaa ggtcggggta aaatacattt ctacccgcag catcggctgc gatcacattg
     2101 atacgactgc cgccgagaga atgggaatct cggttggcac ggttgcgtat tcgccggaca
     2161 gcgttgcgga ttatgctttg atgctgatgc tgatggccat acggggtgca aaacccacca
     2221 tgcacgccgt ggcgcaacaa gatttcagat tggatcgtat ccgggggaaa gaactggggg
     2281 atatgactgt gggagttatt ggaaccggcc atatcgggca agcggtcgtc aaaaggctgc
     2341 ggggatttgg atgccatgtg ctggcctatg ataacagccg aaaaatggat gcagattatg
     2401 tccagcttga tgagcttcta aaaaacagcg atattgttac gctccatgtg ccgctttgtg
     2461 cggatacccg ccatctgatc ggtcagaagc aaattggaga gatgaagcaa ggcgcatttt
     2521 taatcaacac cgggcgcgga gcgcttgtcg ataccggggc gctggtggag gcgttggaaa
     2581 gcggaaagct gggtggtgcg gcactggatg tgttggaggg cgaggatcag tttgtttata
     2641 ccgattgctc gcagaaagtg cttgaccatc cctttttgtc gcagctttta aggatgccga
     2701 atgtgatcat cacaccccat acggcgtact acactgagcg tgtgctgcag gatactacag
     2761 aaaaaacaat caggaattgt ctcaattttg aaaggagttt acagcatgaa tagaataaaa
     2821 gtcgcaatca tcttcggcgg ttgctcggag gaacatgatg tgtcggtaaa agccgcaata
     2881 gaaattgctg cgaacattga tacggaaaaa ttcgatccgc actacatcgg aattacaaaa
     2941 aacggtgtat ggaagctatg caagaagcca tgtacggaat gggaagccga cagtctcccc
     3001 gccatactct ccccggatag gaaaacgcat gggctgcttg tcatgaaaga aagcgaatac
     3061 gaaacacggc gtattgatgt ggctttcccg gttttgcatg gcaaatgcgg ggaggatggt
     3121 gcgatacagg ggctgtttgt attgtctggt atcccctatg tgggctgtga tattcaaagc
     3181 tccgcagctt gcatggacaa atcactggcc tacattctta caaaaaatgc gggcatcgcc
     3241 gttcccgaat ttcaaatgat tgataaaggt gacaagccgg aggcgggtgc gcttacctac
     3301 cctgtctttg tgaagccggc acggtcaggt tcgtcctttg gcgtaaccaa agtaaacggt
     3361 acggaagaac ttaacgctgc gatagaagcg gcaggacaat atgatggaaa aatcttaatt
     3421 gagcaagcga tttcgggctg tgaggtcggg tgtgcggtca tggggaacga ggatgatttg
     3481 attgtcggcg aagtggatca aatccggctg agccacggta tcttccgcat ccatcaggaa
     3541 aacgagccgg aaaaaggctc agaaaatgcg atgattacag ttcccgcaga cattccggtc
     3601 gaggaacgaa atcgggtgca ggaaacggca aagaaagtat atcgggtgct tggatgcaga
     3661 gggcttgccc gtgttgatct ttttttgcag gaggatggcg gcatcgttct aaatgaggtc
     3721 aataccctgc ccggttttac atcgtacagc cgctacccac gtatgatggc cgccgcagga
     3781 atcacgcttc ctgcactgat tgacagcctg attacattgg cgttaaagag gtgataagta
     3841 tggaaaatgg ttttttgttt ttagatgaaa tgttgcatgg cgtccgttgg gatgccaagt
     3901 acgccacatg ggataacttc acaggaaaac cggtggatgg gtatgaggtg aatcgcatca
     3961 tcggcacaaa ggccgtggcg tttgctctgc gcgaggcaca aatccatgcg gctgcgcttg
     4021 gctatggctt gcttttatgg gatggatatc ggccaagaac tgcggtggac tgcttcctgc
     4081 gttgggcagc gcaaccggag gacaatctca caaaagaaaa attttacccc aatatagagc
     4141 gagccgagtt gattacaaag ggttatgtgg cctcacaatc cagccatagc cgtggaagcg
     4201 caattgatct tacgctctac cacctggata caggggaact tgtttcaatg ggaagtaact
     4261 tcgattttat ggacgaacgg tcgcatcata cagcaaaagg gataggggat gcagaggcac
     4321 aaaatcgaag atgcttgcgt aaaatcatgg aaagcagcgg atttcagtct tatcgctttg
     4381 aatggtggca ctataagttg attgatgagc cataccccga tacctatttt aattttgccg
     4441 tttcataatg aaagtattgg ttttgtatca gattttggat ttgataaaat ctatgctggt
     4501 catggacaat cttttactgg aaagtggtta aagcatcttt tgtaatacat tgcaaattat
     4561 agtattcaat ctgcccagcg gcagaaaaga aaaaaaccgc tgttgggcag acctttccca
     4621 cgcccatttt gtattttgct ggcggcggtt ttgagaaatc aaggccgccg ttcttttctg
     4681 gcagcaaaag gtgtggcggc ccataggagg atgccgctat gctgttttgc tattgccatg
     4741 ctctaatcca cttccaccac tcaaaaaaag ccttgcccct ccacagggct attccggtta
     4801 tgagtatgta ctataccatg taaatcagca gcatagcaaa gcattttctc gcccggtagg
     4861 tttgcgacct gccgggcgat ttttgtcgtc ttttgcggcc cggctgtccc caaaaaaatt
     4921 ttttgagaaa ttttcaaaaa ggaggtattt agcgggtagc tggcggcctt gtcaaagtct
     4981 tgttagcgga aagggagaga accacctaag tcccccacag aaagggggtg agaagatgga
     5041 tgctatcccc cgcgactatg aagcgcggtg catgttcgat gctttttgca agacggtact
     5101 gcgtaacgag gccaaaagct acttggctga aatgaagcgc cgccgtgacc gtgaagtgtc
     5161 actaagctct ttgtcacagg cagatttgga caagctctgt acagtagatc actaccccag
     5221 cgacagcttt acgttttcgt cgcatggcta tgacctacat atcaacaatg agcttgttgc
     5281 cgaagccttt gccgcgctgc ccagcatgga gcagagtatt ttaatcctgc actgtgttct
     5341 ggatatggca gatggtgaga tcggcggcct tgtgggaatg tcccggagtg ccgttcagcg
     5401 gcacaggaca aataccttat cggagctgcg gaaacagcta aaggcgttga tgccgaaggg
     5461 aggttagacg cggatgaaga acccgaacta caagggcatt gagcttttgc ccctttcggt
     5521 gatcgaggcc gcccgcgctg gggatgccgg agccgtagag cgcgttttgc agtattacag
     5581 cggttacatg aacaagctgt gtacccggac gctctatgac gaaaacggct atccgcatgt
     5641 gtgtctggac gaggccatga agcgccgtct ggaaatcaag ctcatccacg ctatcgtcac
     5701 gaactgacga accggccccg gcagcatgaa acggcttacc ctttccccgt ttcggtgttt
     5761 tcccggtggc ctgatcgcac cttgataaat atgcccgcaa gataacggcg gcgcatcata
     5821 gggcatacgg acacattctg tctgtgccga gccggacggc tggtgcgcca tgatactttt
     5881 ccctacggga aaagcggagc gaggaacacc acgccgcaat gtgagcgtgg cggcttgcgg
     5941 gcgacgacgt acaggcagga ctatgatact cccttatagc cacagtccga gcgttaaaag
     6001 cgtcgcaggc aatgggtagg gccgcgtgag aaccacgcgg gggtgaaagt cccgtggagc
     6061 tgtgccagca gccgtccgtc tatgccccta aatcacacaa acctttgaat ttgtgaaagg
     6121 gggctgcatt atgaaccatg atgtgcctat ttgggaaaaa tacacgctga cgattgagga
     6181 agcgtctaaa tatttccgta tcggagaaaa caaattgcgt aagctggccg aggaaaatcc
     6241 ggcctctggc tgggtcatta tgaatggcaa ccgcatccag atcaaacgca agcagtttga
     6301 gaaaataatt gacactttgg acacaatcta atagtgaaaa agggccgcag atatgttata
     6361 ataatttata agcatatatg cggctctttc cgtcatggaa aggagcatga cgagatgtca
     6421 gaaaaaagac gagacagcaa aaatcgcgtt ttgcggtctg gagagagcca gagaaaagac
     6481 ggacgatatg cttacaaata tgttgatacc tttggaaaac cgcagtttgt ttactcatgg
     6541 aagttagtgc ctacggataa gaccccggca ggtaaacggg aggatatttc actccgggaa
     6601 aaggagaaag aaatccagaa agaccttgac gacgggatag acaccatcgg caagaaaatg
     6661 accgtctgcc agctctacgc caagcagatc aggcaccggg gcaatgtgaa gcacaacacc
     6721 aaactgggcc gggaacggct gatgcgtatt ctggaacaag atcggctggg gagctgcccg
     6781 attgacagtg tgaagctgtc cgacgcaaag gaatgggcgc ttcggatgaa agaaaagggc
     6841 ctttcttata agaccatcaa taacgacaag cgttccctga aagcggcttt ttacaccgcc
     6901 atacaggacg attgcatccg aaaaaacccg tttgacttcc agcttagtga cgttctggac
     6961 gacgatacgg aaccaaaggt gcctcttaca ccagcacagg aagaaagttt tctgtccttt
     7021 attcaaggtg ataaggttta ccaaaagcat tatgacgcaa tcgttattct gctggggaca
     7081 gggcttcgca tttcggaact gtgcggactg acggacaaag atttggattt tgaaaaccgg
     7141 gtaatcatcg tgagccacca gcttttgaga aataccggcg tcggttacta tattgacgaa
     7201 cccaagaccc aaagcggcgt cagaaaaatc cccatgaatg aggaagtgta tcaggcgttc
     7261 cagcgagtga taaaaaaccg caaaggtgca aagccgttca ttattgacgg gtatgccaat
     7321 ttcctgtttc tcaaacagaa cggctatccc atgacggcgg tagattatgg cgggatgttt
     7381 ggcaggcttg tgaagaagta caacaagagc catgaagaag cgttgccgaa aacgacgacg
     7441 ccccatgcta tgcgtcatac attctgcacc cggttagcca atgcgggaat gaaccccaaa
     7501 gccttgcagt acattatggg acattccaat atcaccatga cgctgaacta ttatgcacac
     7561 gccacattcg attccgcaag ggcagaaatg gaacggctgg cagcctgaca caaggccggt
     7621 tttactactc catttactac ttttgagggg gaaatggtga gaggtaataa gaacatttgt
     7681 aagtatctaa caatatcaaa aatgccggaa aagcccggaa acacgggcta taccgacatt
     7741 caagaacttc taaaaagata atctaaaatc ccatataatt ttaaaggaaa attttgtcga
     7801 ttctgaggat ttttttgat
//
LOCUS       DACCXO010000311         7272 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.guided.135, whole genome shotgun sequence.
ACCESSION   DACCXO010000311 DACCXO010000000
VERSION     DACCXO010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.guided.135"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            36..1304
                     /locus_tag="IVY70_003084"
     CDS             36..1304
                     /locus_tag="IVY70_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP7960125.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            1791..2255
                     /locus_tag="IVY70_003085"
     CDS             1791..2255
                     /locus_tag="IVY70_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7960126.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            2239..2868
                     /locus_tag="IVY70_003086"
     CDS             2239..2868
                     /locus_tag="IVY70_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP7960127.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            3222..4700
                     /gene="msr"
                     /locus_tag="IVY70_003087"
     CDS             3222..4700
                     /gene="msr"
                     /locus_tag="IVY70_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP7960128.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            4747..5193
                     /locus_tag="IVY70_003088"
     CDS             4747..5193
                     /locus_tag="IVY70_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP7960129.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(5392..5907)
                     /locus_tag="IVY70_003089"
     CDS             complement(5392..5907)
                     /locus_tag="IVY70_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7960130.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            6024..7226
                     /locus_tag="IVY70_003090"
     CDS             6024..7226
                     /locus_tag="IVY70_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7960131.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         2300 a         1254 c         1515 g         2203 t
ORIGIN      
        1 ttaggaaaag tttcataatt caaaggagaa aaaagatgaa agcaaagaat ttattattta
       61 gcgttattct tttaggaaat ctagcagttg ccaacacaac agtgctagct gaagaatcga
      121 cagaaactgt cgcaactcag acatcgagtt cttcagaaac aggaattgaa aacgatacag
      181 aaagtacttt gcagtcagct actgtctcag aaaatacaat ttcaaccgaa gaaccaacaa
      241 ctgaaacaac agaacaaaca caaaccgaag acagtacatc agcagaagct cagaacaatc
      301 cagagaatgc agaagaaatc acgaaccaat tgacagatgg gcaaacattg acacataccc
      361 atcaagcaga tttgtctgaa ttcgaaaact atactggcta tgctttttat gctcgctcat
      421 ctgctaattc tcaacaagcg ttcattgata gtattgcctc aactgcacaa agtttggcaa
      481 gtgccaatga cttatatgca tcagtcatga ttgctcaagc catcgttgaa agtggctggg
      541 ggaacagtac tttggcttct gcccctaatt acaatctatt tgggattaaa ggaagctata
      601 atggccaatc tgttacgatg cctacttcgg aatatgtgaa tgggcaatgg attacggtga
      661 atgccgcttt tagaaaatat ccttcgtaca aagaatcatt gcaagataac gtaacggtat
      721 tgaagacgac ttcttttcag ccaggcgttt actactattc cggtgcatgg aaaagcaata
      781 cgaactctta caaagatgct acggcttggc taacaggtcg ttatgccact gcgcctaatt
      841 atggttctac attgaacaat gtaatcgaaa cctataatct gacacagtat gatacagcac
      901 caacttcaac ttctccgatg tatcgtctgt ataaccgtca tacaggagaa catttatata
      961 cattgaatgc cggagaaaaa aattatctgc caactgtagg ctgggaatat gaagggattg
     1021 cttggcaagc accaaacagc ggacaacctg tctaccgttt atacaatcct tacagtggag
     1081 accatcatta cacaatggca caatctgaga tcaactcttt aacaaaaatc ggttggcgtt
     1141 atgaaggtct aagtttttat tctggtggtt ccaagccaat ttatcgacta tttaatccaa
     1201 atgaaaaaac tggtacccat cactatacct tgagtgctaa ggaaagagat tggctaacac
     1261 cgatgggctg gagatatgaa ggaataggat tttatggtta ttaaaaagag gtagtgaaaa
     1321 agctgccctg catcaagtag taagaacagc caaacaaaaa cagattttca tgttttttgt
     1381 ttggttgaac ttattaccgc agatgcaagt ttttgaacac cgtttactca aaaaaggttg
     1441 tgacaacatc ttgtcataac ttttttatta ctgtttaagc atactgatcc aatttctaat
     1501 attcgacggg attcttgcaa aaggcgattt cggaaatttt tggcggtgta aaattatcta
     1561 ctgcagaaga gctatcttct atagtttgat aagaatcaat aatcttggtt ttagcgttgt
     1621 gctttcttta tcattttttc cgttcattct acaaaaaatt tcttttctct atacttttct
     1681 attgagagcg ttttttttat tacacagctg attctttgac ttttttcttt ttttattcta
     1741 ttattgacaa gtcaatcgtt gacgagtcaa ttattagaaa ggaggaaatt atgtctttag
     1801 aagagattag tcacctttta tatcaaatca aattagccga tcaaaaaatc gtccagatct
     1861 ttgaagaaaa tatcggtata agtcttacta gatacgagat catgatgaga ctgaaagaag
     1921 aaaatccatt gctacaaagc agtttgcaag aaaagttgca gattgatcaa ggagcagtta
     1981 ctcgtcatct gaaggtattg gaagcaaatg gctatgtttc gcgtcagaga aatcctcaga
     2041 acaaccggga agtgtatgta gcgctaacag aaaaagccca gaagaaaatc aagcattgtg
     2101 aaatggacca taaagataca ggttccgtgc tatccccggc tttcagcgat gtagaaatga
     2161 aacaatttct aacactttta gataaattga atacccaact ttgcaatata acaacagaaa
     2221 cgaaagaggt taataataat ggaacaaaaa aatgattttt caacaattat gactggtaga
     2281 agatccgttc gcgtatatga tgaaacatac aaaatcccac acgaagaaat gctggaaatg
     2341 atcaaagaag cgacgactgc accttcttct gtcaatatga aaccatggcg ctttgtggtc
     2401 gctgaaagcg aggaagcaaa agaaatcttg cgtcctttga tccgtttcaa tacgagacaa
     2461 aatgatactt cttctgcaat ggtcatgatt tttggcgata tgcaatgtta cgaatatggc
     2521 gaagaaatct acaaccaagc ttacgaatca gggaagatgc caaaagaagt cagagatcaa
     2581 caattagctg cgatcattcc ttattacaag agtttttcaa gagaagaaat gaacgatgta
     2641 gtcaaagtcg attctagctt agcggctatg caattcatgt tagttgcgcg tgatcatggc
     2701 tatgaaacaa atccaattgg tggattcgaa gcagatcagt tggcagaggc atttggtttg
     2761 gataaagacc gttatgttcc agttattatt ttatcaatag gaaaagcggt agaagaaggt
     2821 tacgagtctg ttcgtttagc accagaaaag atcactatgt tcgaataaaa ggttttcagt
     2881 tactattcag tagctattgc tttttttgaa tcgtgtgtgg taacctaata aaaaaggagg
     2941 tcggaaaaat gactgcatcg atgaaattac gtttcgaact tttgaataac aactaaataa
     3001 attcaaaaga catgtataaa caacaatttc atggatggag tatgtccatt ttttcgaacg
     3061 acagtagaat aggtgaaagc tatgacagat ttggtgattt agtcatagtt atttcgttca
     3121 gagtaggaaa agatgtgatg cagttttttt gcaaacagat ttttctgctc ttttttagta
     3181 tcgataattg ttgttttaca tgaaatagga gtgtaaaaca tatggaaaat ttagcagtaa
     3241 atataacaaa tctacaagtc agctttggca accagctaga actatctatt gattctcttc
     3301 gtgtctatca gcaagatcgt atagggatca taggagaaaa tggtgtcggt aaatcgactt
     3361 tgctcaaact aatagccggt gaactttttc ccgatcatgg gaaaatccaa acagagatca
     3421 ccttcaacta cctgcctcaa ttaacctatc tcgctgaagc aaaggaccta aatttggaat
     3481 tagccagtca tttccagtta agactggaag aaacttcaga gcggaaatgg agcggagggg
     3541 aagaacgaaa gatcgagttg atacgtcttc tttcttctta tgaacaaggg atgcttctag
     3601 acgagccaac aacccatcta gatagaaaaa gtattgatcg actgattgaa gagcttcgtt
     3661 attattatgg tacgctggtt tttgttagtc atgatcgcta ttttctagat gagctagcat
     3721 cgaaaatctg ggaagtaaaa gacggagaaa tccgagagtt ttcggggaat tatagtgcct
     3781 atctcactca aaaggaattg gagaaaaaga ctcagctacg agaagcagag tcgatcatga
     3841 aagagaaaaa acgattggaa aaatcgatcc aagaaaagaa aaaacaagcg gaaaagttgg
     3901 aaaaagtgtc cagtaaaaag aaaaagcaac aaatcagact ggatcggttg tcttcctcta
     3961 aacaaaaaga cagtgtacaa aaagccatcc aaaagaatgc gaaaacatta gagagaagac
     4021 tccaaaaaat aggagaaaca accaagccgc agcagatgaa acaaatccgt tttccagtac
     4081 caaaatctct tgaactccac agccgctatc ccatcatggg acaaaatgtc caattggaaa
     4141 gaagtgggag aacattactg gtaaatggtg attttcagtt ttctttaggt aaaaaaatcg
     4201 cgattgtcgg tgaaaatggt tcaggtaaga caaccttatt agaacatatc cgcaaacaag
     4261 gagaaggaat ccttctctct ccgaaagtaa gctttcaagt atatcagcaa aagggttatc
     4321 aaatgacatc tgaagaatcc atcattcgtt ttgtcatgag acaaacagag ttttcagaat
     4381 cgcttgtccg tagtttgctg aatcacttag ggtttgctca ggaaactttg acgaaaccgt
     4441 tatgtacatt aagtggggga gaagcgaccc gtctgacgat tgctttgctt tttactaagc
     4501 caagtaatgt gttgctgtta gatgaaccga ctaattttat tgatatggca acgatcgaag
     4561 ctttagagaa gctaatgcaa atatatccgg gaacgatttt gtttacttct catgattctt
     4621 actttgtcga gcgtacggct gatgaagttt atgaaataaa agggcagaaa ataaaaaaag
     4681 tacttacgag aaatttttaa atgtgctacg atgattttcg tgacaaattt aaaggagtgg
     4741 tacagattgc ctacaaataa aaaagaagga ataattttta caacaatgat gtgttttttg
     4801 atggtcttgg ggatgagcgc gtataattta tggctgcatc aagatcttca tttatcaagt
     4861 ttgttgattg gtctagtgcc tggattcgtg gtggctttca ttttggatgt cttcatcatc
     4921 ggtgtgctgg cgaaaaaaat tgcctttaaa ctaccagtaa atccagaaag caaggtccaa
     4981 atgatcttaa ctatttcatg cttgatggtt ttaggaatgg tgacatgtat gtctttattc
     5041 ggtgtgatca tggaacaagg aatacctgat caattattct ctgcttatat gaatgcttgg
     5101 aaaatgaatg tgattgccgc tttaccgttg cagttgatca tcgttggccc gctttcaaga
     5161 agtgtgctaa aagcaattca aagtaatgaa tgatggaaca ctgttatttg aaaaaaggcc
     5221 ttggcatgct ttttgtcata gtctcttgaa tgcagttcta gaaaaatagt gatccgaacg
     5281 ataggttttg gcctgtcgtt cggatcgctt tttcttttcc aactgtattg ctcttttatt
     5341 gaagataatt tgctcttcaa tagatatttc tagaaaggtt ctgtttatcc cttactcaaa
     5401 aaactgctgc atttgttctc ttacttgctg acaccattgg agttcctgct gacaaagcat
     5461 aattctgcgc tggatcacaa cataacgtcc aaattcttgt ttcttgtcta gcgagaggtc
     5521 attcaatttt ttttgatttt tttgcatgat tttgttcagc tgttgttctc tagtgaatag
     5581 cagtccagta gctgttgttt tatcgagcgt cgaaaaagca tagatttttg ctagaaattc
     5641 atctttttgc gtaggagcgg gagtttcctc tttgatccat tcctctacaa gagcacgtcc
     5701 ttcatcagtc aagtggtaga tctttttttg agagtgctcg tctttgtcca aaacggtcac
     5761 ataaccctgc tggtctaact tggcaagcgt tgtatagatc tgactgtgat gggcttccca
     5821 aaaaattgtg atcagctgct tcaattcata cccagaacgt ggtttggata aaagcacgca
     5881 taataatata taggctaaag tattcataaa aatcctacct ctcgtcttat tttctttatt
     5941 atatcacagt aaagtttcca cgttttacat gtgcatattg acatgtaaaa taaatcatgt
     6001 atgatgctaa tagagagaaa gggatggaaa tattggagaa tcaaatagat atacaagaac
     6061 aaacaaatga agaaacaaag gggaaaagtt cagcgctagt tgtagcagct acctgtatga
     6121 tcgcttttat gggagtgggt ctagtagacc caatcttaaa aacgattgct gcacagttaa
     6181 acgctacgcc agctgaaacg accctgttat ttactagtta catgttagtc actggtgtgg
     6241 tgatgctatt tacaggtttc ttatctagtc ggatcggttt gaaaaaaaca ttgatgacag
     6301 gattgtttat catcgttgta ttcgctggtc ttggcggatt atctgggaat atcggaatgc
     6361 tgattgggtt acgtgccggc tggggtgtcg ggaatgcttt atttgtctct actgctttag
     6421 caacgatcgt cagtatttta gttggtaaca cagaaaaagc aatcatgatg tatgaagcag
     6481 cacttggctg cggaatggct gttggccctc tagtcggtgg ggtcttgggc agtggttcat
     6541 ggcgttatcc attttttggg gtagcatttt tgatgttttg cggttttata gccctagcgg
     6601 tacttttacc tgaaactgag aaaccaaaga gaaaagtagg cctttgggaa ggtgtcaaag
     6661 cgttaagcaa tcataaactt cgttcagttg gactaatcgc attgttatat aatttcggat
     6721 tctttacttt acttgcgtat gcgccatttt tattgattgg ctattctgaa ttagaagtcg
     6781 gttttgtatt ttttggctgg ggtgtgcttc tggctattgc ttcgattttc gttgcaccgg
     6841 ctcttgagcg gaagtttacg acttatcgga caattttagc agcatttgtc ttattcatta
     6901 tttgtttgct gttattagga gctggaactt ctatgccagt ccttgtaccg atcagcattg
     6961 tgttagcagg atttttccaa ggaattatca atacactgtt gacgacgatt gctatggaaa
     7021 taccaggtct gcaacgaaac gttgcttcgt ccagctatag ttttgtccgc tttttcggtg
     7081 gagctttagc accatttatt gcaggtgtga ttggtgaaca tattggtgaa agctatgcgt
     7141 tttattttgc aggtgccatt gttcttatca gtgtatgttg tatcatttat caccgtaatt
     7201 attttgctac aaatgaagaa gaataagaaa tcagaggttg tgaaaaagct gtcctgcatc
     7261 aagtcataag aa
//
LOCUS       DACCXO010000312         2769 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.guided.190, whole genome shotgun sequence.
ACCESSION   DACCXO010000312 DACCXO010000000
VERSION     DACCXO010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.guided.190"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            108..191
                     /locus_tag="IVY70_003091"
     CDS             108..191
                     /locus_tag="IVY70_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP7960132.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            316..1053
                     /gene="erm(B)"
                     /locus_tag="IVY70_003092"
     CDS             316..1053
                     /gene="erm(B)"
                     /locus_tag="IVY70_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP7960133.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1316..2179)
                     /locus_tag="IVY70_003093"
     CDS             complement(1316..2179)
                     /locus_tag="IVY70_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP7960134.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2181..2453)
                     /locus_tag="IVY70_003094"
     CDS             complement(2181..2453)
                     /locus_tag="IVY70_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP7960135.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2470..2679)
                     /locus_tag="IVY70_003095"
     CDS             complement(2470..2679)
                     /locus_tag="IVY70_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP7960136.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT          851 a          487 c          443 g          988 t
ORIGIN      
        1 atttgatagt agatttaaaa ctttgcaaca gaaccaaaat tttgtataat aggaattgaa
       61 gttaaattag atgctaaaaa tttgtaatta agaaggaggg attcgtcatg ttggtattcc
      121 aaatgcgtaa tgtagataaa acatctaccg ttttgaaaca gactaaaaac agtgattacg
      181 cagataaata aatacgttag attaattcct accagtgact aatcttatga ctttttaaac
      241 agataactaa aattacaaac aaatcgttta acttctgtat ttatttatag atgtaatcac
      301 ttcaggagtg attacatgaa caaaaatata aaatattctc aaaacttttt aacgagtgaa
      361 aaagtactca accaaataat aaaacaattg aatttaaaag aaaccgatac cgtttacgaa
      421 attggaacag gtaaagggca tttaacgacg aaactggcta aaataagtaa acaggtaacg
      481 tctattgaat tagacagtca tctattcaac ttatcgtcag aaaaattaaa actgaacact
      541 cgtgtcactt taattcacca agatattcta cagtttcaat tccctaacaa acagaggtat
      601 aaaattgttg ggaatattcc ttaccattta agcacacaaa ttattaaaaa agtggttttt
      661 gaaagccatg cgtctgacat ctatctgatt gttgaagaag gattctacaa gcgtaccttg
      721 gatattcacc gaacactagg gttgctcttg cacactcaag tctcgattca gcaattgctt
      781 aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa acagtgtctt aataaaactt
      841 acccgccata ccacagatgt tccagataaa tattggaagc tatatacgta ctttgtttca
      901 aaatgggtca atcgagaata tcgtcaactg tttactaaaa atcagtttca tcaagcaatg
      961 aaacacgcca aagtaaacaa tttaagtacc gttacttatg agcaagtatt gtctattttt
     1021 aatagttatc tattatttaa cgggaggaaa taattctatg agtcgctttt gtaaatttgg
     1081 aaagttacac gttactaaag ggaatgtaga taaattatta ggtatagggc acctctaata
     1141 actaccaatt aattcacctc aaaatgaaat tagaaaaaaa cagcgaaatt tttaaatcta
     1201 tttcttatcg atacaaattc ctcgtaggcg ctcgggaccc caacatgagg aaatgttttt
     1261 ataaaaaaag agtagttacc gttttttggt aactactctt ttcctttttt aaggtttaaa
     1321 tacctggaag tttaggtgtt ttgggtattg gtggtgtagg tagctgcaag ctttccattt
     1381 tttgttgaat tgctttaaat tcaggggttt cttggtgttg attttgaacc attttttgct
     1441 ctattctctc caaagtcggt tgaatttctt tccctgatac tttacgattt aattctcttt
     1501 ctaaggtctc tttcggacta atggaaggcg tttctaagct tgaatagagt ttcactcctt
     1561 ctctattgta aagccttata tcgctaaata agcccgtttt atgaagggtt tctaaattgg
     1621 tcggtaagtt tttgacaaca atatcatgcg cttgttttgg tgttgcccta gctgtcattg
     1681 gatcatctgc atacatggtt tcatatcgtt caatcgttcc taagtatgat tcaattttcg
     1741 gtactgccat gacgtatatt tttgtttcat aacctttaga ttgaagcatt gtggctgttt
     1801 gaatgggaac gtctgttgtt cgtcctgttc cttcaatcac taaattatag ccttgatcgc
     1861 ttaaacggct aataagtgct tctgtcatgc gattcgaata aggggtaacg tgttttacta
     1921 cgtctttttc atagagtttc actaattcgt caaaatttgg gtgctgttgt ttaaaggtat
     1981 cattatcaat gacaataaca ttcccttgtg tttcttcaaa aattgctgat cgcaaactgg
     2041 ttttccctga ccctggttgc ccaccaagta aaaaagcggt tggcgattca accgctagtc
     2101 tatttttagt tagtcgttca acattctttt ctaaacgatc ttcaaattgc ttttcggtga
     2161 agtcagtaat attcgccatg ttaagccact ttctctttat tcaaaatttg ttttgaatat
     2221 tgattcattg accgccaata ctcatgaata gcttgtaatt cttcaaatgg ttgttgaaat
     2281 aaatcaactt cttgtgccac ttctaattga tttaaaagat tcacttctag taaacgagta
     2341 tttttagtat ctagttcatg attcaaaaca taatttaata cccgattata aacacttgcc
     2401 gataactcat taatgatttc aatttcaaat gttttttcat acgtaactgc catttcattt
     2461 tcactccttt taaagtttgt caggtaaata tttccgtata tattcttcta aggcttgatc
     2521 cataacttct tttacgtttc cgccattctt tgctgtttcg atttttatga tatggtgcaa
     2581 gtcggcacga acacgaaccg tcttatcccc cattatatct ttttttgcgc tgattggcgt
     2641 atcatttcgt tttgctttct gtgcgcctaa atttcccacg atcgctcact tctttctatt
     2701 tcttcttact atttatttta tcatcaccta tcacaaatca caagtgattt gtgattaatc
     2761 acttgtgat
//
LOCUS       DACCXO010000313          565 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13706
            SAMN11027186-rid9896023.guided.295, whole genome shotgun sequence.
ACCESSION   DACCXO010000313 DACCXO010000000
VERSION     DACCXO010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027186
            Sequence Read Archive: SRR10694465
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 23-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743218.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743218.1
            Genome Coverage       :: 228x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 03:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,096
            CDSs (total)                      :: 3,039
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 12 of 70
            Pseudo Genes (multiple problems)  :: 8 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027186-rid9896023.guided.295"
                     /isolate="UW13706"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>565
                     /locus_tag="IVY70_003096"
     CDS             <1..>565
                     /locus_tag="IVY70_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP7960137.1"
                     /translation="DFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQI
                     YKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAV
                     ILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKY
                     LIEHYFDNFKVDSIEIIGSGYDSVAYLV"
BASE COUNT          224 a           53 c          103 g          185 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccgaa tcattaaaaa aacattatac
       61 agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg
      121 atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac
      181 tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg
      241 aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa
      301 tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc
      361 tggttttaga attattgaag atttgccaga acatgaatta cacgagggca aaaaagaaga
      421 ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata
      481 tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa tcggtagtgg
      541 ttatgatagt gtggcatatt tagtt
//
